LOCUS       DASVEV010000001         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_583135, whole genome shotgun sequence.
ACCESSION   DASVEV010000001 DASVEV010000000
VERSION     DASVEV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583135"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF222_00005"
     CDS             complement(<1..253)
                     /locus_tag="VF222_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140839.1"
                     /translation="MQTNLIDSLIVKPNTKIRLSDWNPTYLGRIDKEEAERMLNQSLM
                     NQMSNLQYKLFANKSQSLLIILQGISAAGKDSTIRNVMSA"
     gene            1207..1446
                     /locus_tag="VF222_00010"
     CDS             1207..1446
                     /locus_tag="VF222_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140840.1"
                     /translation="MQQKRRTFEEWWNNLPVTLRENSRKMVENDNIDTSSKSVKKINY
                     ILFQLDIMTEKDYAKNKPTFEEFKFWFNSGQLIIN"
     gene            1783..2886
                     /locus_tag="VF222_00015"
     CDS             1783..2886
                     /locus_tag="VF222_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7140841.1"
                     /translation="MTITKNTMYFTIIGFAMIILMASNLSPELSYSEDKVSKSYIVEN
                     SNTLGNNKEYYSVNNSVEDFNFVAVGDFSCNKDAKNTVMNILNANPEILLGLGDYSYE
                     TSAKCWKDIVGGIDPKKMKISFGNHEFEDTSLVKQYMQYTNLDKQYYSFDYNNVHFIS
                     MATESPYEQGSEQFEFVNHDLMKTKTNPSIDWIIVYYHQPMYTSQTHHEGLESFRDLY
                     HSLFNEYGVDLVLQGHIHNYQRTYPLIYNSENPSNPTITQSAILGNGNNEEKSYVEPE
                     GTIFAIIGTGGQKLHGLDDQAYFTANQLEDHGFLELKTTNNGKSLVGQFYSNDGSIIK
                     DEFIIEKSSVDKTFNDNTPNNNPSNLNEIAYKK"
BASE COUNT         1194 a          365 c          406 g         1092 t
ORIGIN      
        1 aagcactcat tacatttctt atagtactat ctttgccagc tgcagatatc ccttgtaaaa
       61 tgattagcaa tgattgactt ttatttgcaa aaagcttgta ttgcagatta gacatctgat
      121 tcattaggct ttggtttagc attctctcgg cttcttcctt atctattctt cctaaataag
      181 tgggattcca atcacttagg cgaatcttgg tatttggttt gactataagg ctatctatga
      241 ggtttgtctg catttataat aacaattaac atgaatgaat taaagaatgt tttttcctaa
      301 tagagaaagt aaaaatggta ataatacctt aattatagtc ttatttatac tactgcaaca
      361 accaataata ctgaaagatt tagttattcg atccatgaaa caaataaggt ttacaaaaat
      421 gaaatatttt aattaatagt ctttatacat taatcaaata tcaagttgac attaataaaa
      481 gattatttac aggaactata tatgaaattg ttcaaaatta taatcattat agatcttgag
      541 tatctaaata ataaacaata agaaaatagt aaattaggag taaggttcta tttacgtcat
      601 gaaaataatt tgtaattatg caatgtatag aatttcaaat attataatca caaataggga
      661 aaagagtaca gtgtctacag aatggaaata ttgatttttc ttggctctaa aaaattaaaa
      721 atgtatagta gtattcttta gagccaagac aagatgatat ttagaggcta taataataac
      781 gtataagaaa ttaattaaca ttatcgatag atataccaaa ggcctatatt gacatactat
      841 atcctatagg aaaatcttat tttatagtaa gggatctatt attagcttga gtttattgtt
      901 tagtgaagtt accaaattag ataaacgagt tagtgtacat cattattgca atttatttat
      961 ataaaattat tgtcttattt aatggttatt taaatcgtga taaataatca tcaattttat
     1021 tttactatta tattcattaa taaaattaca atagcacgta taaaatgtat atctggataa
     1081 ataaaaatca atataatatt caataacatt aacaaccata tagtctaaat actaaagaaa
     1141 atcattatag gcatataact tttataataa ccaagtttaa gactataaaa attaatgtaa
     1201 attattatgc aacagaaaag aagaaccttt gaagaatggt ggaataatct tccagttact
     1261 ttacgagaaa actctagaaa aatggtagag aatgataata tagatacgtc tagtaaatca
     1321 gttaaaaaga ttaattatat tctctttcaa cttgatataa tgactgaaaa agactatgct
     1381 aagaataaac cgacttttga agaatttaaa ttttggttta atagtggcca attgattatt
     1441 aattgaaaaa taatattatt actttagttt atataacatc ttaattcttt atacaattat
     1501 agatagttat aaacaactat aatcaagaaa atttaatatt cttattcttt aatataatct
     1561 atgttactta taattgttat aataaataat tttattagga ttattgtaat tatttatttt
     1621 tatattaaaa ttcttaatgt ctattttatt tattgtttat tcttagtgtc tatatttact
     1681 atatattaaa ctaagttatt agtgatttgt ataccgacaa gtaatctatg atcatgtctc
     1741 ttttatgata tacttgacta tactatatca caagtgatta atttgactat aactaaaaac
     1801 acaatgtatt tcacaataat tggttttgcc atgataatat taatggcaag taatttatct
     1861 cctgaattat cctattcaga agataaagtt agtaaatcat atatcgtgga aaattcaaat
     1921 actttaggaa ataataagga atattattcg gtgaataact ctgttgaaga tttcaacttt
     1981 gtagcagtag gagattttag ttgtaataaa gatgcaaaaa atactgttat gaatatatta
     2041 aatgcaaacc ctgaaatatt attaggttta ggagattact cttatgaaac aagtgcgaaa
     2101 tgttggaagg acattgtggg aggaatagat cctaaaaaaa tgaaaatttc ttttggcaac
     2161 catgaatttg aagacacgtc attagtaaaa cagtatatgc aatatactaa tcttgataag
     2221 caatattatt cctttgatta taataatgta cattttatct ctatggctac agaatcacca
     2281 tatgaacaag gatcagaaca atttgaattt gttaaccatg atttaatgaa gacaaaaaca
     2341 aatccttcaa tcgattggat aattgtttat taccatcaac cgatgtatac ttcacagact
     2401 catcatgaag gtttagaatc tttcagggat ctatatcata gtcttttcaa tgaatatggt
     2461 gtagatttag tattacaagg acatatccat aattatcaga gaacctatcc tttaatatat
     2521 aatagtgaga acccttcaaa tcctacaata acacaaagcg caatactagg taatggaaat
     2581 aatgaagaaa aaagttatgt tgagcctgaa ggcaccattt ttgccataat tggtactggt
     2641 ggacagaaat tacatggatt agatgatcaa gcttatttca ctgcaaacca attagaagat
     2701 catgggtttt tggaattaaa aacaacaaac aatggaaaaa gtcttgttgg acaattttat
     2761 tctaatgatg gtagtataat taaagacgaa tttattatcg aaaaatcatc tgttgataaa
     2821 acttttaatg acaatacccc aaataataat ccatctaact tgaatgaaat tgcatataaa
     2881 aaataaatag aaattaattg acttttttta tctaataata actgatgatt tttacttcac
     2941 atatatagaa aaaccattta cttatctatg aaagtaaact tttaatcttt gcaaagaatg
     3001 aatctctgga gatatgtaaa cgtcttttac aaaatcttaa aaattcttcg tacttta
//
LOCUS       DASVEV010000002         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_554, whole genome shotgun sequence.
ACCESSION   DASVEV010000002 DASVEV010000000
VERSION     DASVEV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..84)
                     /locus_tag="VF222_00020"
     tRNA            complement(1..84)
                     /locus_tag="VF222_00020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Ser,seq:gct)
     gene            complement(145..720)
                     /locus_tag="VF222_00025"
     CDS             complement(145..720)
                     /locus_tag="VF222_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140842.1"
                     /translation="MKSSVYFCFYDLSIQEQEVIYTLLAKTFYVIEEKLSFDDMESQL
                     PNFLSNTDYSITFIELIFPIKFDNYLFQIISVEHWNSIKNIIKEIKHRRGNKPVICIF
                     KFKGNTNQTDFNIIFPIMNSSVRSFEIAIEKVEYLVDIVPTELSSLPPNTFEVFYLYN
                     ESSAKWIPYQAKSIAENFVSTYIYKNGLWEG"
     gene            complement(896..1168)
                     /locus_tag="VF222_00030"
     CDS             complement(896..1168)
                     /locus_tag="VF222_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7140843.1"
                     /translation="MKNRSRNEIIAFILESVANSRNSNGITATKIMYSAFLSYSQLKE
                     YLALLLEKELLEYYSNTQTYKVTSKGTHYLSIHKQLNEMIVTASQF"
     gene            complement(1451..1849)
                     /locus_tag="VF222_00035"
     CDS             complement(1451..1849)
                     /locus_tag="VF222_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140844.1"
                     /translation="MTIESKGELIEVSQINSNLSLDVQLEKKIINSGNKQTIGFFLQD
                     ISNNKTIDAKIEGNIIYLSGMAESFSLNITNNQLYNYSWIINSKIQSYGTVLLDIYMI
                     YNGTKSNVANLSFSIDDNYNKNKNDRNKHK"
     gene            complement(2007..2633)
                     /locus_tag="VF222_00040"
     CDS             complement(2007..2633)
                     /locus_tag="VF222_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140845.1"
                     /translation="MHNILCDLLTIDGLNTLLFYNPYAKLCFTSTITSYFRKTTYLDF
                     DTTYTAYTKSNLIKNYALNDISLFTPNEGELESLITKIVSNISNSSLLIIDSLNSFYN
                     LYYKKIDMESLKGISTIQHTLSNFLMILLKFCQSLRIPILVTNMMRYRKKKEWTKSPT
                     NKRFLQKKSTVMLFVELLSQNIFFLDIITHPTLLPTTLTLDNNNINIK"
     gene            2675..3856
                     /locus_tag="VF222_00045"
     CDS             2675..3856
                     /locus_tag="VF222_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7140846.1"
                     /translation="MLDDIKQQIIKDFPITIRKIYLNNGSIAPLSLSSIKSITDFLIK
                     YSEQGPDSDDLSKYMEKLIEETRLRIKHLINCNAIEVSFTQSTTQGLNLVANGIEWKK
                     GDIIILRGGRHEHYANYFPWLYVAKRKKLKIKVLKIDQNGFFDIDEFEKLAKLKNSKL
                     IVLSHVLYNNGSIMPVVEVGKIARDNNLLYCIDSAQSVGSLNVDIKRIGCDFMAFPSF
                     KWICGPLGIGIFYCSEKSKDAIIPDSIGGESGQIISDDSLVHNDIPQKFETGFRNYPA
                     LAGLESSLRYILRIGIHKIMSINSKTSSILRDELVKIDGIDIHGIDDKKLRNSIVSFS
                     SPKIESNLLKVKLEKEGIIMAERDLLNGKKIVRASPHFFNNEEEIMKTIRVIKNILAH
                     M"
     gene            complement(3860..4114)
                     /locus_tag="VF222_00050"
     CDS             complement(3860..4114)
                     /locus_tag="VF222_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7140847.1"
                     /translation="MPTAYVLINCDLGSEDDIIKELKKLPEVVEVSGVFGVYDIIVRI
                     RSDSMDKLREVVTWNVRRIDKVRSTLTMMVIEGQGDKDIN"
     gene            4292..5353
                     /gene="rtcA"
                     /locus_tag="VF222_00055"
     CDS             4292..5353
                     /gene="rtcA"
                     /locus_tag="VF222_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX7140848.1"
                     /translation="MLKINGSFGEGGGQILRSTISLSVLLQKPVEIYNIRLNRPKSGL
                     RAQHIHTIKVLADIFNARVENLTLGSQSLRFTPNPDKSYFDYSSKSIKVDIGTAGSIS
                     MVLQALIPSVALSKKLIDIQIIGGTDVRMSPTIDYIRYVFSEAYKIMGINFNIDVIRR
                     GYYPKGGGIVNAKIFPCKNLGLIDLIDYQRIDPKIVGICSNLPRHVLDRQISSAILTL
                     ERNGLFCNSYVASIENAISPGASILVYSKSDFGPYIGGDSIGKINKSAEYVGKEASTK
                     FLSNYNKKVAVDQYLADMLVIPASLANGRSRYLVGEVTEHLMTNLYIISKIVGCKYSI
                     IPHGKNYIVNIDGNPCLNN"
     gene            complement(5590..6183)
                     /locus_tag="VF222_00060"
     CDS             complement(5590..6183)
                     /locus_tag="VF222_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7140849.1"
                     /translation="MMKSRMYDSTKLNFTRLLKLFEDSLDDVIAITRAFSIAYVIHRG
                     KFYDNIKSEPYINHTLRVALILANELKVKDRDVIIAAILHDAFEKTYTNTFQEDLLKK
                     TIGEKAFNLILSTTKFNIKEMESNEYCNLVLKSSQIIRYLILANRLDSIRILKNSIRK
                     DKIQRFKKETQEYFLPIANLTDDNLVFKLSVAMYELK"
     gene            complement(6589..>6822)
                     /locus_tag="VF222_00065"
     CDS             complement(6589..>6822)
                     /locus_tag="VF222_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140850.1"
                     /translation="PAKKATTKRKTTTTTPPAKKATTKRKTTTTPPPAKNTTTKRKTT
                     EFNELQKSSENVLESPVESPVESPVEQTQLETP"
BASE COUNT         2508 a          816 c          980 g         2518 t
ORIGIN      
        1 cgcggggagt gggattcgaa cccacgagtt ctttcgaaca tgggattagc aatcccacgc
       61 cctaccaggc taggcgaccc ccgctcaaag atagtacttg tatgaaatat actactgttt
      121 aaaaaataaa gacatattaa tttattatcc ttcccataaa ccatttttat aaatatatgt
      181 acttacaaaa ttttctgcaa tgctttttgc ctgatagggg atccatttag cagacgattc
      241 attatataaa taaaaaactt caaaagtatt aggtggtaaa gaacttaatt ctgtaggtac
      301 tatatctacg agatattcaa ctttttctat tgcaatttcg aatgaacgaa ctgagctgtt
      361 cattataggg aaaataatat taaaatctgt ttggttggta ttacctttaa atttaaaaat
      421 acatataaca ggtttatttc ctcgcctatg ttttatttct tttattatat ttttgataga
      481 attccaatgt tcaactgaaa ttatctgaaa gaggtaatta tcaaatttaa ttggaaaaat
      541 tagttcgata aatgtaattg aataatcagt atttgaaaga aaatttggaa gttggctttc
      601 catatcatca aatgataatt tttcttctat aacatagaat gtttttgcta aaagagtata
      661 aattacttct tgctcttgaa ttgaaagatc ataaaaacag aaatatactg aggatttcaa
      721 gaaatattat atatattaat tggataagtc aaaatttata gtttcctatc taaaagtatt
      781 aaaaacatat atcatggttg agtattgttg aatattgcat aacaaagaaa tctttttacc
      841 attatatttg caatagaaac aaaaaataaa ataaaatgat agtttgtaaa tttaattaaa
      901 attggctagc agtaactatc atctcattta gttgtttatg gattgataaa taatgagtac
      961 cttttgaagt cactttataa gtctgtgtgt tggaataata ttcaaggagc tctttttcta
     1021 gtaataatgc aagatattct ttcaactgag aataagataa gaatgcacta tacataattt
     1081 tggttgctgt aattccatta ctgtttctgc tatttgctac cgattctaat atgaaagcta
     1141 taatttcatt tctgcttcta ttcttcatgt ttattaatat ttacatacat atgttaagat
     1201 taagaaagaa ttaatttaaa aaaatagaga gaatatggat ttttgaaatt ctacaagaag
     1261 tatatgtaat ataatagaaa gtattgaaga tattaaagta ttatagtaaa tttttttata
     1321 tttaattaaa ataatgaatt gagatatagt attgataatg tacacatcat agtgaatata
     1381 gtgatggata tctactcaat tttagcataa attaattaaa tttaataatt acaattgcat
     1441 ttaactatag ctatttgtgt ttatttctat catttttatt tttattgtaa ttatcgtcta
     1501 tagaaaatga taaatttgca acattagact ttgttccatt atatatcatg tatatgtcca
     1561 ataatacagt tccgtaagat tgaattttag aattaattat ccatgaatag ttatataatt
     1621 gattgttagt gatattaaga gaaaatgatt ctgccattcc tgataaatat attatattgc
     1681 cttctatttt agcatcaatt gtcttattat tagaaatatc ttgtaaaaaa aatcctattg
     1741 tttgtttatt accactatta ataatttttt tttctaattg aacatctaat gaaagatttg
     1801 aattgatttg tgaaacttct attaattctc ctttagattc aattgtcatt aagaaaaaag
     1861 caactactat taatattata gaatatataa tccagaaagg agtcaatata tttaattagt
     1921 aaaaataatg atgctataaa tcttagtata taaaatatca ataactacgt taacatgtat
     1981 ctatagaaat atttgtgatc attttactat tttatattta tattattatt atccaaagtt
     2041 aaagttgtgg gtaacaaagt aggatgagtt ataatatcaa gaaagaatat gttttgactt
     2101 agcaattcta caaataacat cacggtactt ttcttctgta gaaatctctt atttgtagga
     2161 cttttggtcc attccttctt ttttctatac cgcatcatat tagtaactaa aatcggtatc
     2221 cttaaagatt gacagaattt caataatatc attaaaaagt ttgaaagagt atgttgaatc
     2281 gtggaaattc cttttaagga ttccatatca atttttttat aatataaatt atagaagctg
     2341 tttaatgaat caattatcaa taatgaagaa tttgaaatat tagaaactat tttggttatt
     2401 aatgactcca attctccttc atttggtgta aacaaagata tatcattaag ggcataattt
     2461 tttattaaat ttgatttagt atatgcagta taagttgtat caaaatccag ataagttgtt
     2521 tttctaaaat aagaggtaat tgttgatgta aaacaaagct tagcataggg attataaaat
     2581 aacaatgtgt ttaatccatc aatagttaaa agatcacata aaatattgtg caactataca
     2641 tgaattaaat atgttgaata tataaaatgt aatattgtta gatgacatta aacaacaaat
     2701 tattaaggat tttccaatta ctataaggaa aatttatcta aataatggct caattgctcc
     2761 attatctctt tcttctataa aatctattac agattttctt ataaaatatt cagaacaagg
     2821 ccctgactca gatgatttgt caaagtatat ggaaaaattg atagaagaaa ctaggttaag
     2881 gataaagcac ttgataaatt gtaatgcaat agaagtaagt tttacacaaa gcactacaca
     2941 aggactaaat cttgttgcaa atggtataga atggaaaaaa ggagacataa taattcttag
     3001 aggtggaaga catgaacatt atgcaaacta ttttccatgg ttatatgtag ctaagaggaa
     3061 aaaattaaaa ataaaagtat taaaaataga tcaaaatgga ttttttgata tagatgaatt
     3121 cgagaaatta gctaaattaa aaaattcgaa attaatagtt ctgagtcatg tattatataa
     3181 caatggttct ataatgcctg tagtagaagt tggaaaaatt gcaagagata ataatttact
     3241 ttattgcatt gattcagccc aatctgttgg ttctttaaat gtagatatca aaagaattgg
     3301 atgtgatttc atggcattcc ctagttttaa atggatatgt ggtccgttag ggataggaat
     3361 tttttattgt agtgaaaaat ctaaagatgc tataattcca gatagcatag gaggagaatc
     3421 tggacaaatt atttcagatg attctttggt tcataatgat attccacaaa aatttgaaac
     3481 aggatttcgt aattatcctg cattagcagg tttagaatca tcgcttagat atattttaag
     3541 aatagggata cataaaataa tgtcaataaa ttcgaaaact tcatcgattt tacgagatga
     3601 gttggtaaaa atagatggta ttgatattca tggaatagat gataaaaaac tgcgtaattc
     3661 aatagtttct ttttctagtc caaaaattga atctaatctg ttaaaggtaa aacttgaaaa
     3721 agagggaatt attatggctg aacgagattt actgaatgga aaaaaaattg taagagcatc
     3781 tccacacttt ttcaataatg aagaagaaat aatgaaaaca attagagtaa taaaaaatat
     3841 tttggcgcat atgtaattgt taatttatat ctttatctcc ctgaccttca attaccatca
     3901 tagttaaggt tgatctaact ttatctattc ttctaacatt ccaagtaaca acttcgcgca
     3961 atttatccat tgaatcagaa cggattctaa ctataatatc ataaacacca aatacaccgc
     4021 ttacttcaac aacttccggt aatttcttta attccttaat tatatcatct tcagatccta
     4081 gatcacaatt tattaaaaca tatgcagtgg gcattacaag atattataat agtgatctat
     4141 taatcctttt agtttaaata tccctctctc tatgttaaaa ggctattaac aattagtagt
     4201 taatttttaa aatattaatg atagaataca cggataactt tgatttatca caataaattt
     4261 aattattaat tttactacaa attaaatagt attgttaaag ataaatggtt cttttggaga
     4321 aggaggtgga caaatactta gatctactat atcactttca gtacttttac aaaaaccagt
     4381 agaaatatac aatattagat taaacagacc aaaaagtgga ttgcgagcac aacatatcca
     4441 tacaataaaa gtactagcag atattttcaa tgccagagtt gaaaatctca ctcttggaag
     4501 ccaatctcta cgatttacac ccaatccaga taaaagttat tttgattata gttctaagtc
     4561 aattaaagta gatattggta ctgcaggaag tatatctatg gtattacagg ccttaattcc
     4621 atctgtagct ttatctaaaa aattaattga tatacaaatt atcggtggaa cagatgttag
     4681 aatgagccct acaattgatt atataagata tgtattctct gaagcctata aaattatggg
     4741 aataaacttc aatattgacg taataagaag aggatattat cccaagggag gaggaattgt
     4801 taatgcaaaa atatttcctt gtaaaaactt gggtttaatt gatcttattg attatcaaag
     4861 aattgatcct aaaattgtag gaatttgttc taatctacca cgacatgtat tagatcgtca
     4921 aatatcttct gctatattga cactagaaag aaatgggtta ttctgtaata gctatgtagc
     4981 atctattgaa aatgctattt cacctggagc ctcaatttta gtttattcta aatctgattt
     5041 tggaccttac attggaggag attccatagg aaaaataaat aaatctgcag aatatgtagg
     5101 aaaagaagct tcaactaagt ttctcagtaa ctataataaa aaagtagctg ttgatcaata
     5161 tcttgcagat atgttagtaa tacctgcaag tttagcaaac ggtaggtcaa gatatttggt
     5221 aggagaagtt actgaacatt taatgacaaa tctttatatt ataagcaaaa tagttggatg
     5281 taaatatagt attataccac atggcaaaaa ttatattgtc aatattgatg gaaatccatg
     5341 tttaaataat taaaattgat aaattcagtg aaaagtttaa ttgatgttat aattgtcata
     5401 aagattattc gtttcctgaa aattcgtttc ctgaaaatga gaattaatta atatagttga
     5461 gtttctttat atttaatatt aggtctgatc aaagaaaaag tttattgatg ttatatttgt
     5521 agtaatattg aacataaatt aattagaata tttaaaaaat ttgaataata caaactacta
     5581 tgttcaaagt tattttaatt cgtacatggc aacagataat ttaaaaacta aattatcatc
     5641 agttaaattt gcaattggta aaaaatattc ctgggtttct tttttaaatc gctgtatctt
     5701 gtctttgcga atagaattct ttaaaattct aatactatcc aatctatttg ctagtataag
     5761 atatcttata atttgggaac tttttaaaac tagattacaa tactcattgg attccatctc
     5821 ctttatgtta aatttagttg tggataaaat taaattgaat gctttttctc caattgtctt
     5881 tttaagaaga tcttcctgaa aagtatttgt atatgtcttt tcaaaggcat cgtgaagaat
     5941 agcagcgatt ataacatctc tatcctttac ttttaattca tttgcgagaa tgagtgcaac
     6001 tctaagagta tgatttatgt acggttcaga ttttatatta tcatagaatt ttcctctatg
     6061 aataacatat gcaatactaa atgctctagt tattgctata acatcatcaa gtgagtcttc
     6121 aaataatttt agtaatctag taaaattcaa tttagtagaa tcatacatac gcgatttcat
     6181 cataagatac tccagctaga aatgtgcaca atatataaaa taactgtgaa attttaacta
     6241 atatagattt tgataaaact aattaaaaat cacagatgaa aatagatata tttttaattg
     6301 tgtaacctca caatattatg ttaattgtgt aagtacttta ctttaacggg tgttgaacta
     6361 gaaatgattt acaggtttca atatagggga atgaatgact aatataccat atattaaaat
     6421 tattaatcat aatgatgtaa ttagatattt ataatttaca taatcaactt taagttgtca
     6481 cgctggttat atttgtaact atataatata aaaataagaa aaaatagttt gataaactaa
     6541 atggtgatta ccatatacta gatatcatca taataatgta tattaatttt aaggagtttc
     6601 taattgagtt tgctctactg gactctctac tggactctct actggactct ctagaacatt
     6661 ttctgatgat ttttgtagtt cattaaattc tgttgttttt ctttttgtag ttgtgttctt
     6721 tgctggtggt ggtgttgttg ttgtttttct ttttgtagtt gctttctttg ctggtggtgt
     6781 tgttgttgtt gtttttcttt ttgtagttgc tttctttgct gg
//
LOCUS       DASVEV010000003         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1360383, whole genome shotgun sequence.
ACCESSION   DASVEV010000003 DASVEV010000000
VERSION     DASVEV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360383"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..684)
                     /locus_tag="VF222_00070"
     CDS             complement(268..684)
                     /locus_tag="VF222_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7140851.1"
                     /translation="MYNDFIRGSKSIHIATKKVGIKRLLVVGGAGTLEIKPGVQLVDT
                     PEFPTETKQGAMGARDYFNILRKEDDLDWTLLIPAITMTKDTAGIRTGKYRIGINQPV
                     IDQKGESKISVEDLAVALLDEVEKNQFIKQSFTVAY"
     gene            1305..2291
                     /locus_tag="VF222_00075"
     CDS             1305..2291
                     /locus_tag="VF222_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7140852.1"
                     /translation="MDQNGSISQLQFSKYRNTQVLHILTWVISGVLLFFLFNTNVYDV
                     FAQDQNNVTIKTTKLSDSLYMLEGLGGNILLSIGQDGAFMVDDQFAPLTLKIKDAISK
                     LTDKPIKFLINTHWHPDHTGGNENLGELGSIIVSHDNVRKRLNTEQFLEHFNITVPAL
                     SDKGLPIITFSDNMTIYQNGDEIKIMHLDNGHTDGDSAVYFTKNNIIHVGDDFSDKTY
                     PFIDIRGGGSIDGLISSLKTISSIINDETKVVSGHTGISNKTKVNEYVNMLTDVRNQI
                     SQMITEGKTLEDIISLQPTSKYDKIYYSHTIVRPNDYPLKAFVINVYQSLTR"
     gene            complement(2311..2436)
                     /locus_tag="VF222_00080"
     CDS             complement(2311..2436)
                     /locus_tag="VF222_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140853.1"
                     /translation="MPNSDFNMRVEPEDLTNVILFLCSEEAKTITGAAIPTFGVA"
BASE COUNT          936 a          411 c          396 g          923 t
ORIGIN      
        1 cggcattact gtcttagagt accattaaga aagtggaaac aataacataa tcaaaatatt
       61 aagaaaaaat gtagattaat atgaagataa atatcatgat atgggaacaa atgattttct
      121 tatctcagtc atgcaaaagc atgaaaaaat gggttggatg ttaagagctc atcttgatca
      181 agcttaaact tttctttttt tattatacta ctgaaagcat taatagattt taagattata
      241 atttgaacta aaatcaaatg cgaacgttta atatgctaca gtaaaacttt gtttaataaa
      301 ctgatttttt tcaacttcat ctaatagtgc tactgcaaga tcttctacag aaattttgct
      361 ctcccctttc tgatcaatta ctggctgatt aatacctata cgatatttac ccgtacgtat
      421 accagctgtg tctttagtca ttgttatggc cggaatcaag agtgtccaat ctagatcatc
      481 ttctttccgt agaatattaa agtagtctct tgcacccatt gcaccttgct ttgtttctgt
      541 gggaaattca ggagtatcta ccaattgtac accaggtttt atttccaaag tacctgctcc
      601 acctactacc aacagtcttt ttattcctac ttttttggta gcaatatgaa ttgattttga
      661 acctctaata aagtcattat aaatattggg attcttccat ccaggattat atgcacttcg
      721 atagcatggc cttcgttata atctatttcc caaatctcat tgtttatcat aatatttcta
      781 gtggattgtc attgtttgca ttagaattca ttatgtaact acatattcaa ttatctatac
      841 attattataa tcactattat atttgatata ccatcagacg agagcgaaaa tgttctaata
      901 cttcatgatg gtagacacac gatcttgaaa atgtagaatt attgtcgagc atcgtaaaat
      961 cagatatagt agttataaat aatttcgtag tttctgacct aggattccat ttggtggtat
     1021 gaaaaaaagt agacttggaa gagaactatc aaggtatgat atgctaaaat ttgtaaccat
     1081 aatgccaatt agactgtatg acccgttgat atatgaacac tatgtagaat gaggatgaat
     1141 ttgtatagtc taattgtgct tatatattga gcatgtgata ctttttgtct atcatgtaat
     1201 actataagat tttcttaaat tcaaacttta acagattcaa taatttttgt aatacaatac
     1261 gatagaatcc ttttatatgt ttaatcggta ttatattatt aaaaatggat caaaatggta
     1321 gtattagtca attacagttt tccaagtatc gtaacacaca agttttacat atattaactt
     1381 gggtaattag tggagtacta ttattttttc ttttcaacac taatgtttat gatgtttttg
     1441 cacaagatca aaataacgtt acaatcaaaa caacaaaatt aagtgatagt ctatatatgc
     1501 tggaagggtt aggaggtaat attctactat cgattggaca agatggagca tttatggtag
     1561 atgaccaatt tgcccctctt accctaaaaa ttaaagatgc aatttccaag ttaacagata
     1621 aaccaataaa atttctaatc aatacccatt ggcatccaga tcatactgga ggaaatgaaa
     1681 atcttggtga attaggttca ataatagtat cacatgataa tgttagaaaa cgccttaata
     1741 ctgaacaatt cttggagcat ttcaatatta cagttcctgc attgtcagat aaaggtttac
     1801 caattattac tttctctgat aacatgacta tctaccaaaa tggcgatgag attaagataa
     1861 tgcatttaga taatggtcac acagatggtg attctgcagt ttactttaca aaaaataata
     1921 taattcatgt tggcgatgac tttagtgaca aaacatatcc atttatagat atacgaggtg
     1981 gtggcagtat agacggatta atttcatcgt taaaaaccat ctcctcgata attaatgatg
     2041 agactaaggt agtttctggt catactggaa tatctaacaa aaccaaagtt aatgaatatg
     2101 taaatatgtt aactgatgta cgtaaccaaa taagtcaaat gataactgaa ggcaaaactt
     2161 tagaagatat tatttcttta caaccaacct caaaatatga caaaatatat tattcccata
     2221 ccattgtaag accaaacgat tatccactaa aagcttttgt gataaatgta taccagagtc
     2281 ttaccagata ataaaaagat aataataaaa ttaagctact ccaaaagtag gtatagcagc
     2341 acctgttata gtttttgctt cctctgagca gagaaataat attacatttg tcaaatcctc
     2401 tggctctacc ctcatattaa aatctgaatt tggcataaaa tgactttata tctaacgtat
     2461 gaccaataaa tagtagtata tattatgaat caatcccatg accaaacaaa agttgctatt
     2521 gttacaggca gctcttctgc agttatttta aattcaataa ggaaaaagaa atttcattag
     2581 tcttgtccca taatcttttt tagtagactc tgctttaatt gcatatttaa ataatgtata
     2641 tatgatgtca attctttatt gtgtat
//
LOCUS       DASVEV010000004        15757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1439, whole genome shotgun sequence.
ACCESSION   DASVEV010000004 DASVEV010000000
VERSION     DASVEV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VF222_00085"
     CDS             <1..730
                     /locus_tag="VF222_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7140854.1"
                     /translation="PDQELKHSANFLYMVFGRKPTFEEVKVFDICLILHAEHSFNAST
                     FAAREIASTRAHIYSCICGALGALSGELHGGANVEVMKMLFDIHDSKNIEKWIQDKLK
                     EGKRIMGMGHAVYRTTDPRADILERLAKSVCKDDKYKWYEITRQVEQYTKKYMTEIRK
                     QPIYPNVDLYSASLYYCMGFPVDLNTPIFAISRISGWAAHVIEEKFAEAAPKPALYRP
                     KAVYVGKYCGPMGCEYTPMKKRKL"
     gene            815..1297
                     /locus_tag="VF222_00090"
     CDS             815..1297
                     /locus_tag="VF222_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEX7140855.1"
                     /translation="MKINMLSKKETSLTLEKLHTNWPEKTIPKIKNIKAYEIEKDRKL
                     LIHEDNFTAVQIHETILPYLGNNNLVELFPYVLIDMGAVKYICNGANIARPGIVKFES
                     FLKDKIVIVKDKEHQKPLAVGTSLYDSEKASELSKGHIINNMHYVGDKFWNLYKGLQL
                     "
     gene            complement(1309..1977)
                     /locus_tag="VF222_00095"
     CDS             complement(1309..1977)
                     /locus_tag="VF222_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7140856.1"
                     /translation="MFLRGGIRTGIITNIFGESGSGKSQFCFSICANLLKKKEDAKII
                     FIDTSGNFRPERILDITDNPNLNKTLYNIQYIRPYSLKDQFETIKKIYQIKPKLVVID
                     TITSLIAIERKNIARHLMLMKFIHELAHTAINTNCAIVTTNMVRNIIYENGVYKNSNK
                     TEEQKNKIKLQREFMEKSVSLSIHIKLKFEILSISNNFYKVSFLQPFINESTLFRITR
                     EGVL"
     gene            1976..2368
                     /locus_tag="VF222_00100"
     CDS             1976..2368
                     /locus_tag="VF222_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0000398 - mRNA splicing, via spliceosome
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U6 snRNA-associated Sm-like protein LSm6"
                     /protein_id="HEX7140857.1"
                     /translation="MSRLGDPVDINSNKEYYYKYCYNYLTIKEPFYSARCENELLSDI
                     LTLSANSSSQPPKRPLTLLQKSLNQRVAVRLKSEIEYKGVMNNVDSYMNLILVNAEEY
                     LGPDLLANYGKVVIRGNNVLFIRLIKDF"
     gene            2398..3540
                     /gene="metK"
                     /locus_tag="VF222_00105"
     CDS             2398..3540
                     /gene="metK"
                     /locus_tag="VF222_00105"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7140858.1"
                     /translation="MVSKYLFSSESVTEGHPDKMCDQISDAILDEFLSIDANSRVAVE
                     SLTTTGTVFIAGEVTSKGSVDLQKVVRNTIREIGYNSADLGFDCNSCAVFTSIHEQSN
                     DISLGVTPTELKDQGAGDQGLVFGYASEETPEFMPLPIIMAHKLCMKLSEVRKRKELD
                     WIRPDGKSQVSVVYEDFIPKRIDTVVLSTQHSPDIQLDQLREEIIKKVINPICAEWID
                     SHTRFIINPTGRFVLGGPSADTGLTGRKIIIDTYGGMGRHGGGAFSGKDPSKVDRSAC
                     YMARYIAKNIVAAELARKCEVQIAYAIGMAEPVSVMVDTFGTGRIEQEQIEDKVKKNF
                     DMRPSKIIETLSLKRPIYKKTAAYGHFGRNDPSFTWEKTDRVDLLK"
     gene            complement(3600..4472)
                     /locus_tag="VF222_00110"
     CDS             complement(3600..4472)
                     /locus_tag="VF222_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX7140859.1"
                     /translation="MPSLKDSASIVKKIEHMEYQVLKVFVSSIKHHEIINNHNILSYS
                     KLHKDRVEYALESLLKLKLIAKTEKGFKLLTGGLDVYALKILVDSGIILGIGNSLGIG
                     KESDVVEAVSEIDQKRAVKFFRIGRISFTDTKRKRSLEKNKNVNNWLLINIEAAKREY
                     DFLVKLNSTKMNIATPYFRSMHSIVMNRINGVRLIEIRNIQDPLEIFYKVFKQIKIAY
                     KENIINGDLSEYNILLDDNNDIFIIDWPQAVTLEHPNAEFLIRRDIHNVIRYFTRKYD
                     LNIDENSCYNSLLK"
     gene            4655..5854
                     /locus_tag="VF222_00115"
     CDS             4655..5854
                     /locus_tag="VF222_00115"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX7140860.1"
                     /translation="MRNEPNLKVDKITVDDDADLKLAEMEAEGELSLEIIEKRKKGLL
                     RTGYTTGTSASAATKASLLALISSQIYNTVEVVLPKGKMAKLKIAWIRFSQNSQSVTS
                     AVIKDAGDDPDVTHGAEICSTVSLTIEKGRIEIDGGLGVGRVTRPGLGLEIGQAAINP
                     VPKKMIEQVVLDSAKSILPYNGIKVIISVPKGVELAKKTDNPRLGIVGGISILGTTGI
                     VFPYSTASFAASIRQSLDVAISLGTTTVVLTTGGRSEDYIKKIYDTLPEYAFVQMGDF
                     SGYTIKQCANRNIKKIIIAGFIGKLTKMAMGIKQTHVRGSHVSMEYMANLASVCINSE
                     NIINDIRKANTARHVSEIIIQNNIQNFFNLLCKQVYFEMYNHSNKSMEIEVVMFGFDG
                     KITGKYP"
     gene            6015..7082
                     /locus_tag="VF222_00120"
     CDS             6015..7082
                     /locus_tag="VF222_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX7140861.1"
                     /translation="MKEKIAVLAITKNGMRIADLIKKNIISAEIYVPHKFYNEDTNVI
                     WFEEPTQKKIQDLFQSYDALICIFSLGAVIRLISRLLQDKKTDPAVLVIDDKATFVIS
                     TLSGHLGGANALSRYIAAFLNSIPVITTAADVNKTISVDLIGNELGWIIDNFENVTKV
                     SAHMVNDEKIALFQDVGERTWWDTENLPSNVITIKDIEKIKDDSYKGGIIITDKIITD
                     NAILAKSVIYRPKTLVIGIGIHWDTTKEDILNGIKSTFDKYGLSFSSIKSLASIKKNI
                     PVQGLAEFSKEFKLPLYLFEQKELEQISVPNPSDTVKKFEGVASVSEASSILQSQGTL
                     LIPKQKFPPNLTIAVCRVNSR"
     gene            7079..7834
                     /locus_tag="VF222_00125"
     CDS             7079..7834
                     /locus_tag="VF222_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX7140862.1"
                     /translation="MRKALLIIDRGSKELEVQEELVKISNRAKEKGGYIYSNFCFLEV
                     IPPYIDEGIRNCLNKNVDLITVMPYFLYPGMKLKDAVKRTAAISKEKRVKLVITKPLC
                     YSEILTFVIVDRLFKLKFSNDIQLKDKECDVLVIGHGSSDRRATEAFIFTINAIKQYY
                     RNVEYCFLELEGPTIQEGINSIIKKNPKIVLIMPYFLHKGIHIKNDVIKEVKTALKSH
                     TFTNILIADHIGVDDKMVSLILQRANEAEKRIV"
     gene            7827..8504
                     /locus_tag="VF222_00130"
     CDS             7827..8504
                     /locus_tag="VF222_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX7140863.1"
                     /translation="MFKKKMTENPDNRSMSIKSFGIEKNSFDIIDGEIGNHSYNKEFE
                     WDIVRRVIHATADFDFASKEKLLFNNDAIKFGFKAIENKRNVITDVEMIRAGISKNSL
                     TQLNLKCICNISDSNIIHEAKRLNKTRAETAMRNLVQEMKGGIIVIGNAPTALHEVIK
                     MVNEKITIPSLVIGVPVGFVSAVESKIELSKSCIPHITNIGRKGGSSVACSIINALML
                     LYLHRSD"
     gene            8575..9009
                     /locus_tag="VF222_00135"
     CDS             8575..9009
                     /locus_tag="VF222_00135"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7140864.1"
                     /translation="MKELTTEIQINSRPEILWKVITDFKNYYQWNPILQKIKGEPLIG
                     NQLEVHLRTLGGKNRVYHPKVIKITPDRELRWKGTFFLSQIFSGERIFLIEKLSENTV
                     NFVNKEIFSGIGVRFTPKKMEDDIVASFKKMNEALKKTIEQI"
     gene            9185..9466
                     /locus_tag="VF222_00140"
     CDS             9185..9466
                     /locus_tag="VF222_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX7140865.1"
                     /translation="MKAIWNEVILADSDLTVVIEGNHYFPPESINRRFFTESSTHTRC
                     GWKGIASYYTIKIEDDYNQDSAWYYPEPLDKARSIKNYVAFWKGIKVEE"
     gene            complement(9520..10548)
                     /locus_tag="VF222_00145"
     CDS             complement(9520..10548)
                     /locus_tag="VF222_00145"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7140866.1"
                     /translation="MKALKLLSDKTISLVEIDEPIIQDKEILINLGSCGICGSDIRNV
                     FADSCKPSSKLGHEISGTIIKVGKSFNGKKYEGKRVFVNHHAPCNSCHYCNHGNETMC
                     DRFTDNIFPCGISEKIILSEWIVNNKSIFELPDSMSFEEGSMLEPLACCIRGWKKISF
                     VKGDSVLIFGIGPIGILHAMLAKIYGASAIYCIDINQIKVEYCKKMNLGICINGKTSE
                     IDSYINLNDKPSPDVIIIATSDMSVLCKSIDIIKKGGTILIFGEPQKEIKVNIDINTI
                     YSKEIKIITTYAATTKEIHEALRIITNMEIDVNKIITHRYSIAESKDAFEKVHDRNDV
                     IKAIIINK"
     gene            complement(10625..12052)
                     /locus_tag="VF222_00150"
     CDS             complement(10625..12052)
                     /locus_tag="VF222_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX7140867.1"
                     /translation="MPIPTIIIAGTTSGVGKTSITLGILYILKNLGFKVQSFKIGPDF
                     IDPSYHHFVTNSPSYNLDSWLMGKKGLINTFEKYTIGKDIAVIEGVMGLFDGAGGKNN
                     FASTAQIAKILNSPIILVIDASKAARSIAAIAYGFMKFEKGLKIKGIILNKISSNRHF
                     HFIKDAFENKIKIPIVGVIYRNQELIFSERHLGLIPREELDDKRKKDILNSAKILSES
                     LDAEKIVSLIRPLQNEKVRKKKIDPVKEQNMQSIKIAVALDESFNFYYQENLDTLQKK
                     GAQLIFFSPLNDLRLPEEVDGIIIGGGFPEILARNLSRNQSMMRAIKKIAESQIPIYA
                     ECGGLMYLSKSIKEGTSGYKNEQIPESKVNFNKTYRMIGLIDAMTKMTDQLTLNYTQG
                     KVINESLFGKLNRVRGHEFHFSVMENISNDSKFSYYLNKGKGITNQKDGIVVYNTLAS
                     YTHLHFSNTKLPERFILNCKKITRK"
     gene            complement(12196..13917)
                     /gene="uvrC"
                     /locus_tag="VF222_00155"
     CDS             complement(12196..13917)
                     /gene="uvrC"
                     /locus_tag="VF222_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7140868.1"
                     /translation="MSTFNLNKEGKKIPSYPGIYLMKDGTGKIIYIGKAKNLKNRIKS
                     YCNLNQNNTFSNKDKNFWKTRILINKVQDIEFILTDNETEAFLLESNLIKRYRPVFNI
                     ELKDQQRYTYLKITDEKFPRLLVARRNRNGDFQGPKGDIFGPFVYGSSRFLSLGILRK
                     IFKIRICNRLPKKPCLEYFIHNCDAPCINKISEEKYLKNITTLKKILNEKNGLENFIS
                     SLKIEMDAESKKQNYERAKEIRDTVKTIESLLINQKIDLKNRTNEEYIGTKTDTLSGV
                     SHILIFKRINGVISDQRRYEYELVGDNNFSTFLLQYYMTSPQIPYEIYVNENPESKTT
                     LESALGKLSSHNVKIVKVTDSTNNEKKDLMNLILKNLSIQVEKKYNQSIIELKNILNL
                     KEFPFIIDCFDVSNFGITYAVGACVRFVNGYPYKNGYRKFKIRTLQFKQDDYSMIKEI
                     VQRKYSFNKIEQEDEKINNNNNNNSNTTNINNNKIDNALPNLIVIDGGKGQLSSAEIV
                     LKKMGLNIPLISLAKENEEIYTTSSPEPLQISKKNTALHLLQSVRDEAHRFGLAYNIK
                     LRAINLK"
     gene            complement(13928..>15757)
                     /gene="uvrA"
                     /locus_tag="VF222_00160"
     CDS             complement(13928..>15757)
                     /gene="uvrA"
                     /locus_tag="VF222_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7140869.1"
                     /translation="MSTKQINVILYGTNQNIYYKYESKYSQSKWEYESNFEGVIPNLQ
                     RLYNITESENKREMLARFMNERVCDKCSGKRLKKESLSIKLSEKSIMDVGDLPIDECY
                     NFFTTLNLDSTKKYIAKNVLKEIRSRLEFLINVGLNYLTLNRSTSSLSGGESQRIRLA
                     TQIGSNLTGVLYVLDEPTIGLHQRDNDRLIKTLKRLRDLGNSLIIVEHDEDVIRNSDW
                     IIDIGPGAGIHGGKIVAQGNLNEIIENENSLTGQYLKGEKTATLTSKKNPSTKLITIY
                     GAKENNLKAIDVDIPIGCMTIVTGVSGSGKSTLINDILFKAVSSYFGSNDIPGKHDSI
                     SGIDNIDKVIGIDQSPIGRTPRSNAATYVNAFTPIRELFSKTITAKEMGYNPGRFSFN
                     VKSGRCESCEGCGVKRIEMQFLPDVYVRCNDCLGKRYNSETLLVKYKGKTIDDILNLT
                     VEEAMDFFQNIPIIYKKLKTLFDVGLGYIKLGQPATTFSGGEAQRIKLAAELSKRDTG
                     KTLYILDEPTTGLHFEDVKKLLRILNRLTNLGNTVVVIEHNLDVIASADWVIDLGPEG
                     GIDGGQLVIEGPPEKIINYDDKSYTAKFLKKKILNSKPIEINR"
BASE COUNT         5355 a         2405 c         2388 g         5609 t
ORIGIN      
        1 accagaccag gaacttaaac attctgcaaa ctttctttat atggtatttg gacgcaagcc
       61 tacttttgaa gaagtaaaag tttttgatat ttgtttaata ttacacgccg aacatagctt
      121 taatgcgtcg acttttgcag cacgagaaat tgcttctact cgtgctcata tttattcatg
      181 tatttgtggt gctttaggag ccttatcagg cgaattacat ggaggagcta atgttgaagt
      241 aatgaaaatg ctttttgata tacatgactc aaaaaatatc gaaaaatgga tacaagataa
      301 actaaaggag ggaaaaagaa ttatgggaat ggggcatgca gtttatcgta ccacagatcc
      361 aagagcagac atattagaac gacttgcaaa atctgtttgt aaagatgata aatacaaatg
      421 gtacgaaatc actcgacaag tggaacagta tacaaaaaaa tacatgacag agattagaaa
      481 acaaccaatc tatcctaatg tcgatctata tagtgcgtca ctttattatt gtatgggttt
      541 tcctgttgat cttaacactc ctatttttgc gatttcgaga atttccggat gggcagcaca
      601 tgtcattgaa gaaaaatttg cagaagcagc accaaaacct gctttatatc gcccaaaagc
      661 agtatatgtt ggcaagtatt gtgggccaat gggttgcgaa tatactccaa tgaaaaaaag
      721 gaaactataa ccatcttatc ttttgtatgt acatgtctgt cttagtcaca tgaaaattaa
      781 agataaatta tcatttaaag agtaaagata tagtttgaaa ataaatatgt tatctaaaaa
      841 agaaacatct ctgaccttag aaaaactcca tacaaattgg ccagaaaaaa ctatcccaaa
      901 aatcaaaaat ataaaagctt atgaaattga aaaagataga aagttattaa ttcatgaaga
      961 taattttact gctgttcaaa ttcacgaaac aatacttcct tatttgggaa acaataattt
     1021 agttgagtta tttccttatg ttctcataga tatgggtgca gtaaaataca tctgcaacgg
     1081 tgcaaatatc gcacgtcctg gcattgtaaa gtttgaatct tttttaaaag acaaaatagt
     1141 tattgtaaaa gataaggagc atcaaaaacc gttagcggta ggtacttcac tttatgatag
     1201 tgaaaaagcc agtgagttat ctaaaggtca tataataaat aatatgcatt acgttggcga
     1261 taaattctgg aatttataca aaggtttaca attataatct agtatttttt atagtacacc
     1321 ttccctagtt attcgaaata gggtactctc attgatgaag ggttgaagga aggatacttt
     1381 gtagaaatta ttggatatgc ttagtatctc aaattttaat ttaatatgga tagaaagaga
     1441 tacggatttt tccataaact ctcgttgcaa ttttatttta tttttttgtt cttcagtctt
     1501 attactattt ttataaacac cattttcgta tattatgttt cttaccatat tagtggtcac
     1561 tatagcacaa ttagtattta ttgcagtatg tgctaattca tggataaatt tcattagcat
     1621 taaatgtcga gctatatttt ttctttctat ggctatgagt gaagtaatag tatcaataac
     1681 tactaatttt ggttttattt gataaatttt tttaattgtt tcaaattgat cttttaaaga
     1741 atagggtctg atgtactgta tattatatag tgttttattt aaattcggat tatctgttat
     1801 atcaagaatt ctttctggtc taaaattacc tgaagtatct ataaaaatta ttttggcatc
     1861 ctctttcttt ttcagcaaat ttgcacaaat actaaaacaa aattgagatt tgcctgaacc
     1921 actttctcca aagatattag ttattattcc tgtacggatt cctccgcgaa gaaacatgtc
     1981 tagattggga gatccagtcg atattaactc caacaaagaa tattattaca aatattgcta
     2041 taattattta accataaagg aaccttttta ttctgctaga tgtgaaaatg aattgctaag
     2101 tgatatattg acattgtcag ctaattctag ttctcaacca ccaaagagac ctttaacttt
     2161 attgcagaaa tctcttaacc agcgagtagc agtgagatta aaaagtgaaa ttgaatataa
     2221 aggagttatg aataatgtag attcatacat gaatcttatt cttgttaatg ctgaggaata
     2281 tctaggtcca gatttgctcg caaattatgg taaggttgtt attcggggta ataacgttct
     2341 ttttataaga ctcataaaag atttttaatt ttaagttaaa gataaagcct atacgaaatg
     2401 gtttctaaat atttatttag ctctgaaagt gtaacagagg gtcacccaga caaaatgtgt
     2461 gatcaaattt ctgatgcaat actagatgaa tttttgtcca ttgatgctaa ttctagagtc
     2521 gcagttgaat cattaacaac tacaggaact gtctttattg caggagaggt cacatctaag
     2581 ggttctgtag accttcaaaa agtagttaga aatactataa gggaaatagg ctataatagc
     2641 gcagatttag gatttgattg taatagttgt gcagttttta cgtctattca tgagcagagt
     2701 aatgatattt ctcttggagt aaccccaacg gaactaaaag atcaaggtgc aggggaccaa
     2761 ggattagtct ttggatatgc tagcgaggaa actccagaat ttatgccact accgataatc
     2821 atggctcaca aactttgtat gaaattaagt gaagtgagga aaagaaaaga attagactgg
     2881 atacgaccag atgggaaatc tcaggtttcg gtagtatatg aagattttat tcctaaacga
     2941 attgatacag tagttctctc tactcaacat tctcctgata tacaactcga ccagttgcgg
     3001 gaagaaataa ttaagaaggt tatcaaccct atctgtgccg aatggataga tagtcacacc
     3061 agatttataa ttaatcctac tggaaggttt gttttaggag gaccctctgc tgatactgga
     3121 cttaccggca gaaaaataat catagataca tatggtggta tgggcagaca tggaggagga
     3181 gcattttctg gcaaagatcc atcaaaggta gacaggtctg cctgctatat ggcacgatat
     3241 attgcaaaaa atatcgtggc tgcagaatta gctcgcaagt gtgaagttca aatcgcttac
     3301 gctataggaa tggctgaacc tgtttcagtt atggttgata catttggcac tggtagaatt
     3361 gaacaagagc aaatagagga taaagtaaaa aagaactttg atatgcgtcc ttctaagata
     3421 atagaaactt tatcattaaa aaggcctatt tacaaaaaaa ctgctgctta tggtcatttt
     3481 ggtaggaatg atccctcatt tacgtgggaa aaaactgata gggtcgattt gttaaaataa
     3541 cgaattttct ccttttttaa accaagagag gattttccag aattgtatac cgttaatttt
     3601 cattttaaca gactattata acaagaattt tcgtctatgt ttaggtcata ttttcttgta
     3661 aaatatctta ttacattatg tatatctctt cgaataagga attctgcatt tggatgttct
     3721 aaagtgactg cttgaggcca atctattatg aaaatatcat tattgtcgtc taaaagaata
     3781 ttatattcac ttaaatcacc attgatgata ttttctttat atgcaatttt tatttgttta
     3841 aatactttat aaaaaatctc tagaggatct tgtatatttc taatttctat taagcgtact
     3901 ccatttattc tattcattac tatcgagtgc atagatctga aataaggtgt agcaatattc
     3961 atttttgttg aatttaattt tactaaaaaa tcatattctc ttttcgcggc ttcgatattt
     4021 atcaataacc aattgttaac atttttattt ttttcaagag atctttttct cttagtatct
     4081 gtaaaactta ttctacctat tctaaagaat ttaacagctc tcttttggtc aatttctgat
     4141 acggcttcaa caacatctga ttctttgcca atcccaaggg aatttccgat tcctagtatt
     4201 atacctgaat ctaccaatat tttcaaagca taaacgtcta atcctccagt aagcaattta
     4261 aacccttttt ctgttttagc aattaatttt aattttaata aactctctaa ggcatactct
     4321 actcgatctt tgtgtaactt ggaataagag agaatgttat gattatttat aatttcatgg
     4381 tgtttaatag aagaaacaaa tactttcaga acttggtatt ccatgtgctc tatttttttt
     4441 actatactag cagaatcttt tagtgaaggc aagtcttttt cacaatatct taattatctg
     4501 taaaatttca ttctctttat ttaaagattt caattcagta tatttgcttg cagatataag
     4561 tgcacaatta tatcaataaa gaagatatca attttaagca gatgtaatta tagcatatat
     4621 ataaaataat gataaactaa ccctggggaa taatatgcgt aatgaaccta accttaaggt
     4681 cgataagatt accgttgacg acgatgctga tctgaaattg gccgaaatgg aagcagaagg
     4741 agaactttca ttggaaatta tagaaaaacg taaaaaagga ctattaagaa cgggatatac
     4801 aaccggtacc tctgcttctg cagcgacaaa agcatcctta ttagcattaa tttcatctca
     4861 aatctataat acagttgaag tggttctccc taaaggtaaa atggcaaaac tgaaaattgc
     4921 atggataaga ttttctcaaa attctcaatc agtaacaagt gcagtcataa aagatgcagg
     4981 agatgatcct gacgtaactc atggagctga aatatgctcg acagtatctt tgaccattga
     5041 aaaaggtcga attgagattg atggaggact cggagtcgga agggtgactc ggccaggact
     5101 cggattggaa ataggtcagg ctgcaattaa tcctgttcct aaaaaaatga tagaacaagt
     5161 ggttcttgat tcagcaaaat ccatacttcc atacaatggc attaaagtta ttatatctgt
     5221 accaaaaggc gttgaattag caaaaaaaac agataatcca agattaggaa tagtaggagg
     5281 aatctctatt ttgggaacaa cagggatagt atttccttat tctactgctt catttgcagc
     5341 atcaattaga caaagtctag atgttgccat atcacttggt accactactg tggtactgac
     5401 aacgggtggg agaagcgaag attatataaa aaaaatttac gacactcttc ctgagtatgc
     5461 ctttgtccaa atgggtgatt tctctggcta tacaattaaa cagtgcgcaa acagaaacat
     5521 aaaaaaaatt attatagctg gatttattgg aaagctaact aaaatggcta tgggaataaa
     5581 acaaactcat gtacgtggct cacatgttag tatggagtat atggctaatt tagcatcagt
     5641 gtgtattaat tcagaaaata ttatcaatga cataagaaaa gccaatactg caagacacgt
     5701 ttctgaaata ataattcaaa ataatatcca aaattttttc aatttattat gtaagcaagt
     5761 atattttgaa atgtataatc attcaaataa atctatggag attgaggttg ttatgtttgg
     5821 gtttgatggt aaaataacag gaaaatatcc atgataatga atctcttaaa ttcaaatact
     5881 tggaaataat ataatttatt aaggcattat ttttatttgt tttagaagat tttggatcct
     5941 attctacttt aatcagatat taatagataa aaatatgcaa agaattaatt ttgactaatt
     6001 aataacctaa acggttgaaa gagaaaattg cagttctcgc tataacaaaa aatggtatga
     6061 gaatagctga tcttatcaaa aaaaatatta ttagtgcaga gatatatgtt ccccataagt
     6121 tttacaatga agatactaat gttatttggt ttgaagaacc aacacaaaaa aaaatacaag
     6181 atctttttca atcatatgat gctttaattt gtattttttc tttaggggct gtaataagat
     6241 taatttcaag acttctacag gataaaaaaa cagatcctgc tgttttagtt attgatgata
     6301 aagcaacatt tgttattagt acgctttctg gacatttagg aggagccaat gccttatcta
     6361 gatacattgc tgcttttcta aattctatac ctgtgataac tacagctgct gacgtaaata
     6421 aaaccattag cgtagattta attggtaacg aattagggtg gattatcgat aatttcgaga
     6481 atgtcacaaa agtaagtgcc catatggtta atgatgaaaa aatagcactc ttccaagatg
     6541 taggtgagcg tacttggtgg gatactgaga acctaccttc taatgtaatt acgattaaag
     6601 atattgaaaa aatcaaagac gattcatata aaggaggaat aattattaca gacaaaataa
     6661 ttaccgataa tgcgatcctt gcaaaatcag taatatatag acctaaaact ttagtaattg
     6721 gtataggaat tcattgggac acaacaaaag aagatattct aaatggtata aagtctactt
     6781 tcgataaata tggattgagt ttttcttcaa tcaaaagtct tgcttcgatt aaaaagaaca
     6841 ttcccgttca gggattggct gaattctcaa aagaattcaa gcttcctcta tatctttttg
     6901 aacaaaaaga attagaacaa atttcagtcc caaatccttc tgatacagtt aaaaaattcg
     6961 agggtgttgc tagtgtatct gaagcatcct caatattaca atctcaaggg acattactta
     7021 tcccgaaaca aaaatttcct cccaatttaa ctatagctgt atgtagggtg aattctcgat
     7081 gagaaaggca cttttgataa tagatagagg aagtaaagaa ttggaggtgc aggaggaact
     7141 agtcaagata tccaatagag ctaaagaaaa aggaggatat atctattcaa atttttgttt
     7201 cctggaagtg ataccaccat atattgatga aggaattaga aactgtctca acaaaaatgt
     7261 tgatttgata actgtaatgc cttattttct ctatccagga atgaaattaa aagatgcggt
     7321 taaaagaact gcagcaattt ctaaagaaaa aagagtaaaa ttagttatta caaaacctct
     7381 ttgttattct gaaattctca catttgtaat agtggataga ttatttaaat taaaattttc
     7441 taatgacatt caacttaaag ataaagagtg tgatgttctt gttattggtc atggaagctc
     7501 agatagaaga gcaacagaag cctttatttt taccattaat gctataaaac aatattatag
     7561 aaatgttgaa tattgttttc ttgaactaga gggacctact atacaagaag gtattaattc
     7621 tattatcaaa aaaaatccca aaattgtctt aataatgcct tattttttac acaaaggtat
     7681 acatattaaa aatgacgtaa taaaagaagt taaaactgca ttgaaaagcc atacttttac
     7741 caatattctt attgcagatc atattggagt tgatgataaa atggtaagtc ttatattgca
     7801 gcgagcaaat gaagctgaaa aaagaattgt ttaagaaaaa aatgactgaa aatcctgata
     7861 atagatcaat gtccattaaa tcctttggta ttgaaaaaaa tagctttgac ataatagatg
     7921 gtgaaattgg aaatcatagt tacaataagg aatttgaatg ggatattgtt agacgggtca
     7981 ttcatgcaac tgcagatttt gactttgcta gcaaagaaaa actgttgttt aacaatgatg
     8041 ctattaaatt tgggtttaaa gcaattgaaa ataaacgtaa tgtcattacc gatgtagaaa
     8101 tgatacgagc tggaatttca aaaaattcac tcacacaact taacttaaaa tgtatttgca
     8161 acatctctga tagcaatatc attcatgaag ctaaaagact taataaaaca cgggctgaaa
     8221 cggcaatgcg caatttggta caagaaatga agggaggaat aattgtaata gggaatgctc
     8281 ccactgcatt acatgaagtc attaaaatgg ttaatgagaa gataacaatt ccatctttag
     8341 tcattggagt ccctgtagga tttgtttctg cagttgaatc aaaaatagaa ctctctaaaa
     8401 gttgtattcc tcatatcact aatattggta gaaaaggagg aagttctgta gcatgttcaa
     8461 taataaatgc attaatgtta ttgtacctac atagatcaga ttaatactat aacacttccg
     8521 tgaacctgaa atgcgagcaa atcttaaaaa ctatgataga tattaattta catattgaaa
     8581 gaacttacca ctgaaataca aataaattca agaccggaaa tattgtggaa ggtaattacc
     8641 gattttaaaa attattatca atggaatcct atacttcaaa aaattaaagg agaaccttta
     8701 attggaaatc aattagaggt tcatttacgt actctaggag gaaagaatcg agtatatcac
     8761 ccaaaagtta ttaaaatcac tccagaccgc gaattaagat ggaagggcac attcttcttg
     8821 tctcaaattt tttcaggtga gagaattttt ctaattgaaa aactttctga aaatacagtc
     8881 aattttgtca ataaagaaat attttctgga ataggtgtca ggttcactcc caaaaaaatg
     8941 gaagatgata ttgtggcaag ttttaaaaaa atgaacgaag cattaaaaaa aactatagag
     9001 caaatataaa ataatttatc aggatctaaa ataataattc cattaataat taatagatta
     9061 attcattaat tacttaatta attaatatcg agttactgga catataattc tgatatcaag
     9121 aattgctata tcgttacagt ttagataagt aatcttttaa aggagaacat catttacctt
     9181 aaatatgaaa gcaatttgga atgaagtaat tttggctgat agtgatttga cagtagtaat
     9241 tgaaggaaac cattactttc ctccagaatc aattaataga agatttttta cagaaagtag
     9301 tacgcataca cgatgtggat ggaaagggat tgcgagctat tatacaatta aaatagaaga
     9361 tgattataat caggattcag cctggtatta tccagaacct ttagacaaag ccagaagtat
     9421 caaaaattat gtcgcttttt ggaaaggaat aaaagtagaa gaatgaatta gattgattat
     9481 tttatattca attttatata aactgctcta gatcatccgt cacttattta ttatgatggc
     9541 ttttataacg tcatttctat catgtacttt ttcaaatgca tccttggatt ctgctatact
     9601 atatctatgt gttattattt tattaacatc aatttccata tttgttataa tacgtaacgc
     9661 ttcatgtatt tccttagtag tagcagcata ggtcgttata atttttattt cttttgagta
     9721 aattgtatta atatctatat ttactttaat ttctttttga ggttctccaa agattaagat
     9781 agttcctcct ttttttatga tatcaattga cttacataaa acactcatat cggatgtagc
     9841 aataatgatt acatctgggc tgggtttatc attcaaatta atgtaactat caatttctga
     9901 tgtctttccg tttatacata ttccaaggtt catcttttta caatattcaa ctttaatttg
     9961 atttatgtct atacaataaa ttgctgaagc cccataaatt ttagctaaca tagcatgaag
    10021 aataccaatc ggcccgatcc caaatattaa taccgaatcg ccttttacaa atgatatttt
    10081 tttccatccc cgaatacaac aagcaagagg ttcaagcatt gaaccttctt caaaactcat
    10141 tgaatccgga agttcaaaaa tactcttatt attgacaatc cattctgaaa gaattatttt
    10201 ttctgatatc ccacatggaa aaatattatc tgtaaatcta tcacacattg tttcgttgcc
    10261 atggttacaa taatgacaac tattacaagg agcatgatga tttacaaaaa ccctttttcc
    10321 ttcatatttt tttccattaa aacttttacc aactttaatg attgttccac ttatctcatg
    10381 tcctaacttt gaacttggtt tgcaagaatc agcaaaaaca tttcggatgt cagaaccaca
    10441 aattccacac gatcctaaat taattaatat ttctttatct tgtataattg gttcatcaat
    10501 ctcaaccaat gatattgttt tatctgataa taattttaag gctttcatgc actctctcta
    10561 tctattgtta agattgaata aatttaaatt tattactaaa taaattttcg cccagagatg
    10621 atttttattt tcttgtaatc tttttacaat ttaatataaa tcgctctggc aatttcgtat
    10681 tggaaaaatg aagatgagta tatgaagcaa gagtattata aactacaata ccatcttttt
    10741 gattcgttat tccttttcct ttatttaaat aataagaaaa tttagaatcg tttgaaatat
    10801 tttccattac agaaaaatga aattcatgac ctctaaccct atttaatttt ccgaaaagtg
    10861 attcatttat tacttttccc tgtgtgtaat taagtgttaa ttgatccgtc atttttgtca
    10921 tagcatcgat taagccaatc attctataag ttttattgaa atttactttt gattccggaa
    10981 tttgttcatt tttatagcct gaagttcctt cttttataga cttagaaaga tacattaaac
    11041 caccacattc tgcatatatt gggatctggc tttcagctat ctttttaata gccctcatca
    11101 tagactgatt cctacttaaa tttctcgcta atatctctgg aaatccacct cctattataa
    11161 ttccatccac ttcttcgggt aatctgagat catttaaagg actaaagaaa ataagttgtg
    11221 cccctttttt ttgtaaagta tctagatttt cttggtagta aaagttaaaa gactcgtcta
    11281 atgcaaccgc aattttgata ctttgcatat tttgctcttt aacgggatca atttttttct
    11341 ttctaacttt ttcattttgt agaggtctaa ttaaactaac aattttttca gcatctaaag
    11401 attctgataa gatcttggct gaatttagta tatctttttt tcttttatca tctaattctt
    11461 ctcttggaat taatcctaaa tgtctttctg aaaaaattaa ttcctgattc ctatatataa
    11521 cgcccactat tggaattttt attttgtttt cgaaagcatc tttgataaaa tgaaaatgcc
    11581 tgttacttga tattttattg agaataattc cttttatttt taatcctttc tcaaatttca
    11641 taaatccata tgcaatagca gctattgatc ttgcagcttt actggcatca atcactaaaa
    11701 ttattggaga atttaatatt tttgctattt gagcagtact tgcaaaatta tttttaccgc
    11761 cagccccatc aaataaaccc attactcctt caataactgc tatatctttt cctattgtgt
    11821 atttctcgaa agtattgatt agtccttttt ttcccattaa ccatgaatcg agattataag
    11881 atggactatt agtaacaaaa tgatgataag agggatcaat aaaatctgga cctattttaa
    11941 aagactgaac tttaaaacca agatttttta gtatataaag aattcctaaa gtgatcgatg
    12001 tcttgcctac tccactggta gtcccagcaa ttattattgt aggaatcggc atataaaata
    12061 aggatataaa taaatggata attatctttt tttgttttat tagttaattc tatactctta
    12121 aaatctgttc atcataaggc ttataaagat tatttatgta tgtagatgaa tttttttgta
    12181 gaggaataag gaatattatt ttagatttat tgctcttaat tttatattat atgctaaacc
    12241 aaatctgtgt gcttcatctc ttacagattg aagtaaatgc aatgcagtat tcttctttga
    12301 aatttgcaaa ggctctggag agctagttgt atatatttct tcattttctt tggctaaaga
    12361 aataagtggt atattcagac ccatcttttt taatactatt tctgcggagc taagttgacc
    12421 ttttcctcca tcaataacta taagattcgg caaagcatta tctattttat tattatttat
    12481 attagtggta ttagaattat tattattatt attattaatt ttttcatctt cttgctcaat
    12541 tttattgaaa gaatactttc tctgtactat ttcttttatc attgaataat catcctgttt
    12601 aaattgtaaa gttcttattt tgaatttcct gtatccattt ttatatggat atccattaac
    12661 aaaccttacg catgccccaa cggcatatgt tattccaaaa tttgatacat caaaacagtc
    12721 aatgatgaat ggaaattctt ttaaattgag aatatttttt agttctatta ttgattgatt
    12781 atattttttt tcaacttgaa ttgataaatt cttcaagatt aaattcatta aatctttttt
    12841 ttcattatta gtagaatctg taaccttgac aattttgaca ttatgactcg atagttttcc
    12901 taacgctgat tctaaagttg ttttcgattc aggattttca tttacatata tttcataagg
    12961 aatttgtggt gaagtcatgt aatattgaag taaaaatgtt gaaaaattgt tatcaccaac
    13021 caattcatat tcatatctcc tttggtcact tattaccccg ttaattcttt taaagataag
    13081 aatatgagac acccctgata acgtatctgt ttttgtacct atatattctt cgttagttct
    13141 attttttaaa tctatttttt gattaattaa taaactttct atagttttta cagtatcacg
    13201 aatttccttt gctctttcat aattttgttt ctttgactca gcatccattt ctatttttaa
    13261 acttgaaata aaattttcta gtccattttt ttcattaaga atctttttta atgttgtaat
    13321 attttttaaa tatttttctt cactaatctt atttatacat ggggcatcac aattatgaat
    13381 aaagtattct aaacaaggct tttttggtag tctattacat atccttatct tgaaaatttt
    13441 tctcaaaatt cctaaagaga ggaatcttga actcccataa acaaacggac caaaaatatc
    13501 tcctttagga ccttgaaaat ctccattcct atttcttctt gcaactaata atctaggaaa
    13561 tttttcgtct gtaattttta aataagtgta tctttgttga tcttttaact caatattaaa
    13621 aacaggtcta tatcttttaa tcaaatttga ttcaagaaga aatgcttcag tttcattatc
    13681 ggttaggata aattcaatat cctgtacttt atttatcaat atccgcgttt tccaaaaatt
    13741 tttgtcttta ttactgaagg tattgttctg attgagattg caataacttt taattcgatt
    13801 ttttaaattt tttgcttttc ctatgtaaat tattttacct gttccgtctt tcattaagta
    13861 aattccagga taactaggaa tcttttttcc ctctttgtta aggttaaatg ttgacattcc
    13921 gaattattta acgatttatc tcgatgggct tagaattcaa tatctttttc tttaaaaatt
    13981 tagcagtata acttttgtca tcataattta ttattttttc tggaggtccc tcaattacta
    14041 attgtccgcc atcaattccc ccttctggtc ctaaatcaat gacccaatct gcactagcaa
    14101 taacgtctaa attatgttca atgacaacaa ctgtatttcc caaattggtc aatctgttca
    14161 aaatcctaag caatttcttc acatcctcaa aatgaagccc agtagttggc tcgtccaaaa
    14221 tataaagagt tttgcctgta tcccttttag aaagttcggc cgcaagttta attctttgtg
    14281 cctctcctcc tgaaaaagtc gtagcgggtt gacccaattt aatatatccc aaacctacgt
    14341 caaataatgt ttttaatttt ttgtagatga tggggatatt ttggaaaaag tccatcgcct
    14401 cttctaccgt caaatttagt atatcatcta tagttttacc cttgtatttg accaacaatg
    14461 tttcagaatt ataacgcttc cccaaacaat cattgcatct tacatacaca tcaggtaaaa
    14521 attgcatttc aattcgcttt actccacagc cttcacagga ttcacatctt ccacttttta
    14581 cattaaatga aaatcttcca gggttataac ccatttcttt ggccgttata gttttagaaa
    14641 agagctccct tattggtgta aaagcattaa catatgtagc tgcattagat cgaggagttc
    14701 taccaattgg agattgatca attccaataa ctttatcaat attgtctata ccgcttatgg
    14761 aatcatgttt tccaggaatg tcattggatc caaaataact agatacagcc ttaaaaagaa
    14821 tatcatttat aagtgtagat tttccagaac cagaaactcc tgtgactatt gtcatacagc
    14881 caataggaat atctacatct attgctttta gattattctc ttttgcaccg tatatagtaa
    14941 ttaacttcgt gcttggattt ttttttgaag tgagagttgc agttttctct ccttttaaat
    15001 attgaccagt taaagagttt tcgttctcta taatttcatt caaatttcct tgagcaacta
    15061 tttttccacc gtgaatgccg gctccaggac cgatatcaat aatccaatca gaatttctta
    15121 taacatcttc atcatgttca acaattataa gggaattgcc caaatcccta agtcttttta
    15181 gagttttgat taatctatca ttatctcttt gatgtagtcc tatagttggt tcatctaaaa
    15241 cgtaaagtac accagttaag ttagagccga tttgagttgc aagtctaatt ctttgagatt
    15301 cacctcccga aagtgaagat gttgatctat ttaatgttaa ataatttagt cctacattaa
    15361 ttaaaaattc cagtcgagat ctgatttcct tcaacacatt ttttgcaata tacttttttg
    15421 tagaatcaag atttaatgta gtgaaaaaat tgtagcattc atcaataggt aaatctccta
    15481 cgtccattat tgatttttca gataacttta tagataatga ttctttcttt agtctctttc
    15541 cagaacattt atcacaaact ctttcattca taaatcgtgc aagcatttct cttttgtttt
    15601 ctgattcagt aatattatac aatctttgta aattgggaat cactccttca aaattacttt
    15661 cgtattccca cttgctttga gaatattttg attcatactt gtagtaaata ttttgatttg
    15721 ttccatatag tattacattt atttgttttg tgctcat
//
LOCUS       DASVEV010000005        21310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_2968, whole genome shotgun sequence.
ACCESSION   DASVEV010000005 DASVEV010000000
VERSION     DASVEV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF222_00165"
     CDS             <1..229
                     /locus_tag="VF222_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7140870.1"
                     /translation="PDNTGDQPLRIGANSLNEDKFFTGDIDEIRLWNRALNMSEIKDS
                     YEDNKFNNTGQILFLSYGDYLNSEGKPTYIK"
     gene            626..1387
                     /locus_tag="VF222_00170"
     CDS             626..1387
                     /locus_tag="VF222_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX7140871.1"
                     /translation="MNGYNINIFDTVLLSLLGLLERFLSIFRAKAHSVADRFEDPNEM
                     LNYSFEKQHELINKLRRDITTVVTSKKRLEIQKAKLSTNISTLEQQARRSLESNREDL
                     ARLALERKNLLLSQIKELERQIGDIEKEQHKLEDAERRLSSKIEEFKTRKEMIKAEYS
                     AAEAQVKIKENMTGISEEMNDLGMVLNRVEEKTENMKAKAQALDEMIDSGTLTDITTS
                     YPAGNADIEKELDKVSIDRSVEEELTKLKTEVGKT"
     gene            complement(2283..2498)
                     /locus_tag="VF222_00175"
     CDS             complement(2283..2498)
                     /locus_tag="VF222_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7140872.1"
                     /translation="MTDVAKIIEIVGSSTQGWEDAVQVAITEAGKTIQGITGLEVIDM
                     TGKVDPSTGKIEIYRATVKIAFGVKHS"
     gene            complement(2734..3393)
                     /locus_tag="VF222_00180"
     CDS             complement(2734..3393)
                     /locus_tag="VF222_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140873.1"
                     /translation="MEFFSKIKKTLGILNNDNDKNNNNIDNPFIDSESIAALSLACIK
                     LDENLGMKSTGKCGIFVKSVNSETFKQMKEHVDNFLKIVIDKKNINFDVTFESKIDNY
                     GYLWFIIKGKSIEEIVAVINAIGDLIHEKGFSRQLLANIFEFSSGYGTNNNDTVNNNQ
                     YLIYNYKLDKFYPFVPFSTSPDNKKRNHDQEFRIMKEISDTIKVEENPSLWYPIWNIP
                     F"
     gene            3676..3963
                     /locus_tag="VF222_00185"
     CDS             3676..3963
                     /locus_tag="VF222_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140874.1"
                     /translation="MVILFNSGSDRNPSIYYPSPSQYESPYYPLPSPSIPYDLPYDMP
                     YGDFPDSPSSDDQYPLTPDEDLRSFDSPLDDSFRFEDQYPLTPDEDLRSFD"
     gene            complement(4057..4236)
                     /locus_tag="VF222_00190"
     CDS             complement(4057..4236)
                     /locus_tag="VF222_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7140875.1"
                     /translation="MRNIKSITRSRSIIRQLRKDLETCEQFGDARTAVFLTAVMEKHE
                     KMAWMLRSFIELQNH"
     gene            complement(4386..4673)
                     /locus_tag="VF222_00195"
     CDS             complement(4386..4673)
                     /locus_tag="VF222_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140876.1"
                     /translation="MNFFHHKKFVCEQDKEKFETYEELIDHSRHVHHITILKCNGCGK
                     EFIHEKDRLHHVREEHEKELDRREHKDEYKKVVKSTQEDIDEHSKKFSDNF"
     gene            complement(4944..5543)
                     /locus_tag="VF222_00200"
     CDS             complement(4944..5543)
                     /locus_tag="VF222_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140877.1"
                     /translation="MDKKAFTKTYKHYLICSIIGTILFLTLTSFPMSMSQESSAGSMQ
                     NTTDIASLNLSDKSKEMVQITKTATSSFNIINHSTGLLGTFDNLYTITGSSDSLYKSK
                     DLIITMITSDFDKSPTIGYVRAADIEHVFDLSNESNSLPSALPNPFADEQTINSTLSL
                     VISDAIDSAQGIGFTTVAIQCNFGMNIMDWKCKDHGIYG"
     gene            5925..6101
                     /locus_tag="VF222_00205"
     CDS             5925..6101
                     /locus_tag="VF222_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140878.1"
                     /translation="MSDNSQKDEIKDETHQVQDIEQEELEDDEADEFEEDEDDDFEEN
                     EGEITQDQEKNKSK"
     gene            7543..9510
                     /locus_tag="VF222_00210"
     CDS             7543..9510
                     /locus_tag="VF222_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX7140879.1"
                     /translation="MVEPLERTVSSYVSKQFVMVDENVSVANAVKLTQPKNIQTIIVT
                     SNGKPVGIVTDSDILEKVVIKGDDSDLVFLKSIMTSPILTLNRTATFKQAIELMRIRK
                     IKRVPIVDSHNKSAQIIGVVTQKFLAEAIRNSVIEKTFKNYRVSIKENYKPIFGNLGF
                     IMQFAGILMIVPAIFGTLLNELESATSIYLAVVSMSLTGFLLNTLGEKSTLNLKQSSI
                     VVVSSFTLLSLYGSLPYIYVNPFEINFDYLSLFVSSILESSSGYTTTGISTIENPESL
                     PESFVFYRSYTQWVGGLSFVYLVMALYYPETKLATMRNMIGGGILKFKQLLSTISFIF
                     VIYTFIFILLIFFMGNIQLINAVSLSFTTLVTGGFIPDSSIFNSINSYQLILIMVGML
                     IAAFPFGFHYGIFSKEVRTRRLSMEIIVYLFCVLLFAFLFIIIEPSISTHDWLTSVFH
                     VVSASTTTGFSFINLSSLSLEGKLFLIFVMLVGGTAFSTAGGIKIARLLLIFQKLKGN
                     TIFLSMSDAFTPLSISSTAIQFRKHGNGQKFSIPKKNLLKSKNDRVYRDNQKLSLLSD
                     KAFRDAIFVIILFVILSLGSATAISHLNKSDFVDALFESSSTLSNTGLSVGITTVDLD
                     LISKLILSINMVLGRFEIIALLYIFISRLRR"
     gene            complement(10242..10460)
                     /locus_tag="VF222_00215"
     CDS             complement(10242..10460)
                     /locus_tag="VF222_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7140880.1"
                     /translation="MTIFMVYKYIHIIGTSPNNIDDAVKEAVDEASKTINNIEWAELG
                     RVTFRILDNKVTEFQAEVKIGFKIERKN"
     gene            complement(10526..11389)
                     /locus_tag="VF222_00220"
     CDS             complement(10526..11389)
                     /locus_tag="VF222_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7140881.1"
                     /translation="MLLLSIPLIEKSVTAQKIIDNEVLSPNNDDKGNSNGEGNSNGEE
                     KSNLNEFAEKPLVHVSIEGTEINDKIRGGDGNDVMSGEDGQDTLQGHAGDDEIDGGKG
                     EDNIDGGIGDDEIDGGDDQDTLQGHAGDDEIDGGKGEDNIDGGEGDDGLNGGDENDDI
                     KGGIGDDEIDGGDGSDVVDGGDGKDKLKGGNDADRFVCDQDDKISDYNSLENDIIMGE
                     CEYEDKGLIPKPPLEKSPFPASSGAPNSQDNNNFKSFDSKDSEAPNSQENFFKKFMSK
                     FIDTDVPQILR"
     gene            complement(12170..12364)
                     /locus_tag="VF222_00225"
     CDS             complement(12170..12364)
                     /locus_tag="VF222_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140882.1"
                     /translation="MSKGKSKSKLLSLFNFKEKLSKHKGDDQVTKCKECKLEFNTKER
                     MNAHKKIAHSGRGDKKKKQH"
     gene            13257..13982
                     /locus_tag="VF222_00230"
     CDS             13257..13982
                     /locus_tag="VF222_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7140883.1"
                     /translation="MENGNIVRIPINKESREKIEGNLNIPKSSKSLVIFAHGSGSSRS
                     SPRNQYVSRVLNNDGVATLLIDLLTEREEKTDVVTKEYRFNISLLGDRLIAVTDWILN
                     QDNHLNTFNKKLGYFGASTGSAAALIAADKRSDNINAIVSRGGRVDLALKYTSLREIK
                     CPTLFLVGENDHPVIEWNRQVIEKQLINVGKKKIIIIPQASHLFEEPGKLEEVAKYAS
                     GWFRCYFQIKEKTDNNKTKNNNY"
     gene            complement(14247..15140)
                     /locus_tag="VF222_00235"
     CDS             complement(14247..15140)
                     /locus_tag="VF222_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7140884.1"
                     /translation="MILINPINALLWAIRRNQRDVINLYSSLSPLMSITTGSYMLNFG
                     YWSDGIKNPIEAQEHLCKMIGKLAELESAKTLVDVGSGLSSPAIFWKNEYPLLEIYCI
                     NINHDHLIFANRLKKNYFDQKNSNKKFEKDISLFNCTAIKLPFNKLSIDRIIALESAQ
                     HFRPLIEFIKEARYILKQDGIFVIAIPVITNLLSPIQNLVKLGILSFTWASEHYLLND
                     ILDIVEHEKFKIIEILNIGPLVYNPLSEYYIRNRKILLKRITSEYGSFLEYILFKSIS
                     KMDKLSKEKIIDYVIIKLKKM"
     gene            15761..15976
                     /locus_tag="VF222_00240"
     CDS             15761..15976
                     /locus_tag="VF222_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140885.1"
                     /translation="MNYFRSGNGLLMRLMGYVLITFVIPFVINMIKKKFDDQKRLLCN
                     KCHGKLEVIDTSKFYCKKCKIIRFDHK"
     gene            complement(16387..17463)
                     /locus_tag="VF222_00245"
     CDS             complement(16387..17463)
                     /locus_tag="VF222_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7140886.1"
                     /translation="MDKKYRSTTSIIISLILGIAIIFTLAQQSFSQTEDKKILKIGYF
                     PNINHAQAVIGIGNGDFQKVLGTNVEIKPFIFNAGPSAIEALFAKQIDATYVGPNPAI
                     NGYVISEGKDVRIISGATSGGAVFVIRGDTGINSASDFANKKFASPQLGNTQDVALRN
                     YLLDHGYKTVENQGNVHVLPAKNSDILTLFLKKDIDGAWVPEPWGERLIKDANGKLFL
                     DERDLWADGKFVTGLILVRTDYLKQNPDVIENLLKAHIDKTQWINNNKEEAVKTFNTE
                     LKKITGQTIPEDVLQQSLTRLEFTYDPIQQSLYKSANDAFKLGFLGDTNPDLSNIFDL
                     SILNKVLKEKALVPINSSAIKTVE"
     gene            complement(17623..18612)
                     /locus_tag="VF222_00250"
     CDS             complement(17623..18612)
                     /locus_tag="VF222_00250"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7140887.1"
                     /translation="MLNKILTLDRTDNEKPRLEGITSIIDKLQPLDIENFKNIVSFVD
                     SVKIPQSCFLLYSPAELERRIKFYHEHKISVAIDSKITDYALANNTLNEYLRELLSIG
                     INIIEVELNMNNSFEDNQKIADISNSFDLEVQWKFNKKNSGNNIYNENIFEKIRELFK
                     LSKTKIILEIDQKFDLNIFNRNTNWKLISSLISTYSQSNFIFETDIESLQIILISKFG
                     ERINLGLIDPFQVGLIEWSRRKDYSKVEKSSDQHTTLNVRNILGGPSVKFIYFIIKSF
                     HPIDQTELMRISELPRRTIQASISELKFQGVIFEILDPKDARKKIYELSSPAG"
     gene            18983..19765
                     /locus_tag="VF222_00255"
     CDS             18983..19765
                     /locus_tag="VF222_00255"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7140888.1"
                     /translation="MQIKNQYPRQKVSLSLKNVSKKYTQRNSETSIVLENVNIQILDG
                     EIVTILGKSGCGKSTLLNLIAGFENSYGGEILLNGKPVKDINPERIMMFQESALFPWL
                     TAFQNVEIALKMAKVPKNQREKVVKHYLDIVGLSDYSNSYIHQLSGGMKQRVSLARAL
                     SLNPKILLMDEPFAALDITIKKSLYRELLDLHEKTKKTILFVTHNINEAVLLGDRVIV
                     MSPRLYGIKKEYVIDIPKDQRSDNLSIKNIINEITADLYDTT"
     gene            19927..20634
                     /locus_tag="VF222_00260"
     CDS             19927..20634
                     /locus_tag="VF222_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7140889.1"
                     /translation="MSEILPKLSLPSPLAVGQTITELLLDFTLIKGTAFTLWRLFLGF
                     MLSLTLGLITGLLMIKFQQFGKTMSSFAVGLQSFPSIAWIPFAILLIGFNDFGILFVV
                     VMSSVFSVMLSTYSGLRNVPPIYIRAARNMGAKGFSLFRYVLIPAATPTLIMGIRQAW
                     SFAWHALVGAEMLITTLVGLGYILSVGREFSNMSQIIATMIVIFTIGLIFDRVVFTKI
                     EEKIRDRWGLNQQKIEQ"
     gene            complement(21064..>21310)
                     /locus_tag="VF222_00265"
     CDS             complement(21064..>21310)
                     /locus_tag="VF222_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140890.1"
                     /translation="YVHPEVLVDTQWVEEHLEDKNVRVVESYLPVNKHYYNKKNFRFV
                     LCDSCFWFATILKNVFKVTQCPRCKKNKIYIDRIAIN"
BASE COUNT         7449 a         2949 c         3003 g         7909 t
ORIGIN      
        1 acctgacaat acaggggatc agccattacg aattggtgcc aattctttaa atgaggacaa
       61 attttttaca ggagatattg atgaaataag attatggaat agagcattaa atatgtctga
      121 aataaaagac tcatatgaag acaataaatt taataacaca ggacagattc tcttcttatc
      181 atatggagat tatctcaata gtgaaggaaa gcccacttat ataaaataac catatctttt
      241 tacttttttt agttgtattt atgatattat gctctgttct attgtcaatt aagtaatagc
      301 ttaatttaag ggcgaatatt tgactgttat tttataaaaa tatttgtatg aggaatctag
      361 atatattaaa ttgtaactaa ttatttccaa aaatattgtt acagtttttg ttaccaaaat
      421 taaaaaatca tattttggtt ctccatattc aattaattat attatttgta attttatcat
      481 aaactatctg ggcttataat tattgtcata tattaatttg agtgagaata tgaatgtcta
      541 ctactagtta taagaaataa aaaaaacatt tgcatagaat aggtattaaa tataaaattt
      601 actagcgctt accttatcaa ttgccatgaa tggatataac attaatatat ttgatactgt
      661 tctattatct ttattgggat tattagaacg atttttatcg atttttagag ctaaagctca
      721 ttctgttgct gatagatttg aagatccaaa tgaaatgcta aattattctt ttgaaaaaca
      781 acatgaattg ataaataaat taagacgtga tataactacg gtcgttactt caaaaaaaag
      841 acttgaaata caaaaagcaa aacttagtac aaatatctct acgttagaac aacaagctcg
      901 tcgttcacta gaatcaaata gagaagattt agcaagatta gcactagaac gaaagaattt
      961 gctcctttca caaattaaag aattagaaag acagattggg gatatagaaa aagaacaaca
     1021 taaattggaa gatgctgaaa gacgactttc ttctaaaata gaagaattca agacacgtaa
     1081 agaaatgata aaagcagaat attcagctgc tgaagctcaa gtcaagatca aggaaaatat
     1141 gacagggata tcagaagaaa tgaatgacct tggtatggtg ttaaacaggg tagaagaaaa
     1201 gacagaaaac atgaaggcta aggcacaggc tttagatgaa atgatagatt caggaactct
     1261 aactgacatt acaacatctt atcctgctgg gaatgcagat attgaaaaag aactcgataa
     1321 agtttctata gataggtctg tcgaagaaga attaacaaaa ttaaaaactg aagtcggtaa
     1381 aacttaattt ttattttatt gataaaatac taaaattgta tgaatagcac tgatcttatt
     1441 gtgagaatat ctggtaaatg acaatgtaag ttagttcaga aacattagac ataattgatg
     1501 aatttgataa ttcaacaata gtcgatttga ttcaagataa aataatatca atgatcaagt
     1561 gccatacatt tactactcaa acagaactat atgaaaaagt aacacaaaat catcaattta
     1621 atcaagttaa aagaacaatc atagatgcct aaataatttg ttaaatctga tactatcatt
     1681 ttagattcag atccttttat tgaagaaaca acaaaatttt tagtgaaaaa ggaataattt
     1741 taaatatcta gttttgtatt ttatatataa aaaatattaa tgaaaattac gatcttataa
     1801 tatcttttat tattactttc aattaataat tttaggattg agaaatctat taaaagtaac
     1861 atattattaa gcttaggaag ttaaaagcaa agtataataa tagaaaaatg caaattgtta
     1921 tcctattatg aataaatctt ttattatgaa tgctcttatt attgtgttat taattattgt
     1981 gggcttaata ctactaaaat ttttgaccca attggttata atagttgtta taattggggg
     2041 agcttattta ttttatagac tatatttaaa taataaacgt aagaaatcct ttatataatc
     2101 aaaaatttat tttttttaat cgtctctctt gcatacaatt ttatttttat ttaggaattt
     2161 atcttagtta tattatcttt ttaatatttt ctagcaaaaa tgctttactg gagaaatatt
     2221 tttgtaagga ttctctattt ctagaatttt cctattaaat atcaaaaaaa agaggatgaa
     2281 atttatgaat gttttacacc aaaggcgatt ttgacagtgg cccgatatat ttcgattttt
     2341 ccagtagaag gatccacttt tccagtcata tcaattacct ctaatccagt aattccctgt
     2401 attgttttac ctgcttcagt aatagcaact tgaacggcat cttcccaacc ttgggtcgaa
     2461 cttcctacaa tttctattat ttttgcaaca tcagtcatta tagtttgaag gatgataatt
     2521 tatattattt aagttttttc agtcaaatcg ttttttattt tctagttttt tcattacttt
     2581 tactaataaa tatttttatg ttattatttt taataaataa tataatatta atatattttt
     2641 atacatatat tttaacagta ttttatatct gttataagga tttatgcaat aaagatgatt
     2701 ttatctttac atatatatca tatttatcct tttttaaaat ggaatattcc agataggata
     2761 ccataaagaa ggattttctt ctacctttat tgtgtctgat atttccttca ttatcctaaa
     2821 ttcttgatca tgatttcttt ttttattatc tggtgatgta gaaaatggaa caaatggata
     2881 aaatttgtct agtttataat tatatatcaa gtattggtta ttgtttacag tatcattatt
     2941 atttgtacca tatccgcttg aaaattcaaa tatatttgct aataattgtc gtgaaaaacc
     3001 tttttcatgg ataagatctc ctatcgcatt tattactgcc actatttctt ctattgattt
     3061 tccttttata ataaaccata gatagccata attatctatt tttgattcaa atgttacatc
     3121 aaaattaata ttttttttat ctattacaat ttttaaaaaa ttatccacat gctctttcat
     3181 ctgtttgaaa gtttcagagt ttacactctt gacaaaaatt ccacatttgc cagtactttt
     3241 catacctaga ttttcgtcca gttttataca tgccaatgat aaagctgcga ttgactcgct
     3301 gtcaataaat ggattatcga tattgttatt atttttgtca ttgtcattat tcaaaatccc
     3361 taaagttttt tttattttgc taaaaaattc catgtgatgt tgattaacta taattatttt
     3421 gatttatcct tttctattat ttccctatca gtaaggtaat atttttatat ctcaaataaa
     3481 atgtaattgg ttcttatttt aactgtcaat tttgagggtg agaaagtatc tgtaatatct
     3541 attttattta ttaaataatg tagcaaaggg aaatattaat aatgattaac aattattaat
     3601 gatcatctat tcagattgat tcaccctcaa aaaaaatttt tagttataat atttatcttg
     3661 atagtgatta taggtttggt aattctattt aatagtggtt cagatagaaa tccttctatt
     3721 tattatcctt ctccctctca atatgaatct ccatattatc cattaccatc gccatcgata
     3781 ccttatgatt taccttatga catgccatat ggtgatttcc ctgattcccc ttcatccgat
     3841 gatcaatatc cgcttactcc tgatgaagac ttaagatcat ttgattctcc attagatgat
     3901 agtttccgtt ttgaagatca atatccgctt actcctgatg aagacttacg atcatttgat
     3961 taattgttaa tgaactttta aacaggagat ccagatctga tcgtaaactc tctatttagt
     4021 aaagcacatt aatgttagtg aaagtataaa aaaaatttaa tgattttgta attctataaa
     4081 tgatcttaac atccatgcca tcttttcgtg tttttccata actgctgtaa gaaaaactgc
     4141 tgttcttgcg tctccaaact gctcacaggt ctccaaatcc ttcctaagct gtcttattat
     4201 tgatcgtgat ctagtaatag attttatatt tctaattcat ttttattcaa gaggttattg
     4261 atagtcctca cctcttatta tttcttatac attttacata ttagtttggt acatctatgt
     4321 tatcgttatt aaaatttaag atttaattag acacgaattg tatgtataaa tgaatttcaa
     4381 aaaaattaaa aattatcact aaacttttta gaatgttcat cgatgtcctc ttgtgtactt
     4441 ttgacaactt ttttatattc atctttatgt tctctcctat ccagttcctt ctcatgctct
     4501 tctcttacat gatgtaacct atctttttca tgtatgaatt ccttcccaca tccattacat
     4561 tttaaaattg taatatgatg gacatgtctt gaatgatcaa ttaattcttc ataagtttca
     4621 aatttttctt tgtcttgttc acagacaaat tttttatgat gaaaaaagtt cattaaatat
     4681 cgaatgtaat tcgaatatta aaactatttg tttattatta acgtattggt atggagaata
     4741 tttggtatta aaggaatatt caaactaatt ttcctacttt gaaattattt tttatttggt
     4801 ttaaatacaa ttaaaaaact tatttggaaa aagagaacat caaacatcaa gtttgtatgt
     4861 aagtttgata cagtaccctt tattttttat atatgctttg atctaggtaa tactcgatgt
     4921 caaaataaaa agaaattaaa aatctaccca taaattccat gatccttgca tttccaatcc
     4981 ataatgttca ttccaaaatt gcattgtatt gcaacagttg tgaaaccaat gccttgtgct
     5041 gaatcaatag catctgatat aaccaaggaa agtgttgaat taattgtttg ttcgtctgca
     5101 aaaggattag gtaatgctga gggtagagaa tttgattcat tagataaatc gaatacatgc
     5161 tctatgtctg ctgctctaac atatcctatt gtaggagatt tatcaaaatc acttgttatc
     5221 atggttatga tgagatcttt agatttgtat agagaatcac tacttcctgt gatggtgtat
     5281 aagttatcaa atgttcctaa taatccagta ctgtgattga ttatattgaa tgaagatgta
     5341 gctgttttgg ttatttgtac catttctttt gatttgtcag aaaggtttag tgaagcaata
     5401 tctgtcgtat tttgcattga acctgctgat gattcttgtg acattgacat agggaaactt
     5461 gtcagtgtta gaaacaaaat agttcctatt atagaacaaa ttaggtaatg tttatatgtt
     5521 tttgtaaaag ccttcttatc cataataaat caacattaat ttttaattat ttaaatatac
     5581 ctaccaacct agaattcaat ctaagtcaat ctatcataaa atttttttct cttttttaga
     5641 aagaattttt ttattacatt gttaggacaa tataagagat atgctaatgt attttatcaa
     5701 gttatatgtt tattatattc ttagaattag tagaactatg cataagaatc tagatcaaag
     5761 aaatgaaatg tgttatttaa aattcaggat ttactagtta gtttccctaa tattaaattt
     5821 tattttcacg acaaaagaat atgaaactaa gaaatacaaa gtacaattgc taaaagccta
     5881 acctacttta aaaagttatt aagaagattt atcctattac tataatgagt gataatagtc
     5941 aaaaggatga aataaaagat gaaacgcatc aggtacaaga tatcgagcaa gaggagcttg
     6001 aagatgatga agctgatgaa tttgaagagg acgaagatga tgattttgaa gagaatgaag
     6061 gagaaataac ccaagatcaa gaaaaaaata aatcaaaatg agacggtatt ttgtcaaata
     6121 aattcttaat atttgacttt tagtaattat tatttaatga ataattattt tagttatcga
     6181 tttttgctta gattgtatta tttgtctaaa atagaacttc aatttatgat taatggtcat
     6241 aatattgaat ttaaaatttt ttaatgccaa atgaagtgat gagatttaaa gaagacttta
     6301 ttaattatta attaatattt tttattggtc ttatactctt tacttataaa cctaaatgaa
     6361 attcttctgt tatccattga ttgtaaattt ctttcaaatt cccatcaaat tccattgtaa
     6421 atctgttgta atagtttttt gtggttctta atttattttg taagagatgt ctcaggaagt
     6481 gattcgtata tgatgagaaa taaattatac cgtgaatggt gctagattag atgtgtagaa
     6541 aatataaaaa tagacacctc agagtgtaat ataaaataac agggatgttc atcaaaactt
     6601 agctacctta tgatttcagg aatatagggt ggatgtaatc accgatctgt attttgccaa
     6661 aggaaaatgt agattttcta acccgacaaa ccaagatcta caattttgct gcttccatgc
     6721 attggatatc agtactaggt caggttgaag atgaaattgt aaattgtttc aagaaggagg
     6781 aggagggttt tgtcttccat attcatcgta caaaacggtt aattaagtaa tggaagaagg
     6841 aagtgctcaa atagtacagt ttagtttatt tgagtctata atttcctcta gtaattgcac
     6901 ttgaagacag acttaaaaaa ggaattactg ttcttgacat tgcttgttag taatggtcgg
     6961 gctatgatta tactggcaaa tcaattcccc cacagtaagt tacaggttat gacatttcaa
     7021 aagaagcaat acaaaacgca caatttcaat ctttaacata tgataataaa aatgttgaat
     7081 ttgatgtaaa ggatgtatca aaattttaga tccctcaaaa aggtctatct tttagtttaa
     7141 ttagaccttt gatgccttcc acgatcaagc tgatccttca actgtattaa gaaatattgt
     7201 atgtttattg aaacatcatg agggaatttt tttgatgcaa gatatctttc gtcatctttc
     7261 cttcaaaata atataggtca gccagtggga actttcttta agccgtatca tgtcttcatt
     7321 gtatgagtgt atcgcatgct caagatggaa tagtacttcg tgcaatgtga ggaaaggaga
     7381 aagcagttga aatactcaaa gaagcaggtt ttcgaaggtt gaatttaaaa tcctgcgtca
     7441 agatttccga aattattact atggaggata cttgaattaa gatcatgaca taacattctt
     7501 aatagttatt attgtattaa aaaaaagtct gaaaggatta ttatggttga accattagaa
     7561 cgcaccgtat cgtcatatgt tagtaaacaa tttgtaatgg ttgatgaaaa tgtttctgta
     7621 gccaatgcag taaaacttac ccaaccaaaa aacattcaaa cgattattgt tacatctaat
     7681 ggaaaacctg ttggtatagt gactgatagt gacattctag agaaggtcgt aataaaagga
     7741 gatgactctg atttagtttt tctgaaatct ataatgacat ctccaatttt gaccttaaat
     7801 agaaccgcaa ctttcaaaca agctatagaa ttaatgagaa ttcgaaaaat taaacgagtc
     7861 ccaatagttg attctcataa taaaagtgct caaattatag gagttgttac acagaaattt
     7921 ctcgcagaag caattcgaaa ttcagtaata gaaaaaactt ttaaaaatta cagagtatca
     7981 ataaaagaaa actacaagcc aatttttgga aatctaggtt tcattatgca atttgccgga
     8041 atattaatga ttgttcctgc aatatttgga acattattaa atgaattaga atctgcaaca
     8101 tccatttatt tagcagttgt ttcaatgtcc ttgactggtt ttcttctgaa tacattagga
     8161 gaaaaaagta cgttaaactt gaagcagtct tctattgttg tggtatcgag ttttacactg
     8221 ttaagccttt atggaagttt accttatatc tatgtcaatc catttgaaat aaattttgat
     8281 tatttatctc tctttgtaag ttcaattcta gaaagttctt ctggttatac aactacaggt
     8341 atttctacaa tagaaaaccc tgaatcacta ccagaaagct tcgtatttta tcgatcttat
     8401 acccaatggg tgggtggatt aagttttgtt tatttggtga tggctttgta ttatcctgaa
     8461 accaaattgg ctacgatgag aaatatgata ggagggggaa ttctaaagtt taagcaatta
     8521 ctgtctacta ttagttttat ctttgtaatc tatacattta tttttatttt attaatattt
     8581 tttatgggta acatacagtt gatcaatgct gtatctttat catttacaac tcttgttaca
     8641 ggaggtttta ttcctgattc ttcaattttc aattctatta attcttatca gctaatactg
     8701 attatggtag gaatgctgat tgctgcattc ccatttggtt ttcattatgg tatttttagt
     8761 aaagaggtaa gaactcgaag attaagtatg gaaataattg tctatctatt ttgtgtatta
     8821 ctttttgcat ttttatttat aataatagaa ccttccattt caacacatga ttggcttaca
     8881 tcagtattcc atgttgtaag tgcatctaca acaaccggat tttcatttat taatctatcc
     8941 tctttatcct tagaagggaa actatttctt atttttgtca tgctggtcgg tggaacagca
     9001 ttttcaactg ctggcggaat aaagatagcc agattattat taatctttca gaaattaaaa
     9061 ggcaatacaa tatttttatc aatgtctgat gcgtttaccc cattatcaat ttcgtcaact
     9121 gcaattcaat tccgtaaaca tgggaatggc caaaaattct caatcccaaa aaaaaatttg
     9181 ttgaaatcaa aaaatgatcg agtatatcgt gataatcaaa aactatcgtt attgtctgat
     9241 aaagctttta gagatgcaat ttttgtaatt atattatttg tgatactctc tttgggaagt
     9301 gcaactgcta tttctcacct taataaaagt gattttgtgg atgcattatt tgaatcgtca
     9361 tcaactcttt ctaataccgg tctttcagtt ggaataacta ctgtggacct ggatttaata
     9421 tcaaaactca tcttgtcgat taatatggta ttgggtagat ttgaaataat agcacttctc
     9481 tatattttca tttcgagatt aaggagatag tctgtaaaag tttaaaaatt tcctctaaaa
     9541 atagtggttg tcaattcata caaagagaaa ttaattgaat gtttataaaa agaaaaatta
     9601 tcagaacaaa atccaacctc ctgcccaaat acattttatg gttttattct ctgatataat
     9661 ttatattgca agatatgcct aattttgatc tgtaactgca gaattaatag catttcctaa
     9721 tatcatagtg tttgatttgt tttatttcct tataataata agcttataat aactaggtat
     9781 tataaaatcg tcttgttaaa tttaattatc tataaaatga tgtttctaat acatatctgg
     9841 attaggaagg agtagattac tggttcaaat aaatcagtca ttgataacat gcctttatct
     9901 atttctcttt tttgaggtca tctaagttac ttattccatg ggtggtatgg ttgatcaacg
     9961 acgtattgat gttattttta tttgaggtaa tttaccattt tattttattt atttcagagg
    10021 aacatattga gatttttgtt tgaaaatttt ctattgcttt gttgacttta taaattattt
    10081 tttataaaca tcactcaata ttaattaatt agaatagagt caaatttctt ttattatacg
    10141 ggtattttag ctacaaaatg ttatagtgtt attaaagaag aaaaaaatat cataatatgg
    10201 aaattctaga tcattacaag atccaaaatt taaaatttta attaattttt acgttcaatt
    10261 ttaaatccta tcttcacttc agcttgaaat tcggtaactt tattatctag tattctaaat
    10321 gtaacccttc ccaactctgc ccattctata ttatttattg tttttgatgc ttcatcaact
    10381 gcttctttaa ctgcatcatc tatattatta ggagacgtcc ctataatgtg gatgtatttg
    10441 taaaccataa aaatagtcat tgatcattac ttaaaacttt tattaatttt attaccgtgt
    10501 ttatataaat tataaaatga attgtttatc ttaatatttg tggaacatcg gtatcaataa
    10561 attttgacat aaatttcttg aagaagtttt cctggctgtt tggagcttct gaatccttgg
    10621 agtcaaaact cttgaagtta ttattatctt ggctatttgg agctcctgag cttgcaggaa
    10681 aaggactttt ttctaaggga ggtttaggta ttaatccttt atcctcatat tcgcattctc
    10741 ccataataat atcattttca agggaattat aatcactaat cttgtcatct tgatcacaaa
    10801 caaatctgtc agcatcattt ccgcctttca atttatcttt cccatctcct ccgtctacaa
    10861 cgtcactacc atctcctcca tcaatctcgt catcacctat tcctccttta atgtcatcat
    10921 tttcatctcc tccattcaaa ccatcatctc cctctcctcc atcaatgttg tcttctcctt
    10981 tacctccatc aatctcatca tctccagcat gtccttggag ggtatcttga tcatctcctc
    11041 catcaatctc atcatcacct attcctccat caatgttatc ttctccttta cctccatcaa
    11101 tctcatcatc tccagcatgt ccttggaggg tatcttgacc atcctctcct gacataacat
    11161 cattaccatc tcctcctcta atcttatcgt tgatctctgt tccttctatt gatacatgta
    11221 ccaaaggttt ttctgcaaat tcattaagat tagatttctc ttcaccattg ctattacctt
    11281 caccattgct attaccttta tcatcgttat taggggataa tacttcatta tcaataatct
    11341 tctgggctgt aacgcttttt tcaattaacg gaatgctaag taacagcact aggatgatac
    11401 tacacctgat ttttaagcta cgaattttaa caatatctgt atagtagcat tgatatttaa
    11461 aatctttttg ttactatttt ttgaattaaa tatagtaaaa aagttacatt atatttaaat
    11521 gtaagattca atgatagaaa ttttctatgg tattttttga tagtgaatgg atagtaagaa
    11581 aaatttcata agattttcct taaatttaaa agctaaaaaa ttataacatt tggatatcct
    11641 tcagtcaact tctttattgt aatttaacta ttttttagct cttaagatta ataccgattt
    11701 agtctttcaa attaagaaca ttttaggagg aaaatgtaag taaaatatag gaatagcaac
    11761 aatatatatt ctatattata atgggaatta tctacaatac taagataata ttgttttatt
    11821 tctaaataat tgagataaat atatgtgata tgaaaaatct aaatatatca cagccaaata
    11881 taattcgaca aattttaaat ttctaactct gaatgacatt tatttaacgt ctattaaaag
    11941 taacgaaaaa atgaataaat gaatgaaaaa ttctaaaata atggaactta taaatttagg
    12001 aatgttatcc attcaataag gtaaattaca tttgagatat tacaagtgga ctttactatt
    12061 agtgtgaata cactatacct tatattgaaa taatatttac agtcaattcg ttcaaataca
    12121 attaatcaaa gatttataaa tcttattaag tttagctaaa aaaactaatc taatgctgct
    12181 ttttcttttt atctcctctt cctgaatgtg caattttttt atgagcattc attcgttctt
    12241 tggtattaaa ttccaattta cattccttac acttggtgac ttgatcatca cctttatgtt
    12301 tagaaagctt ttctttgaag ttgaataagc ttaagagttt tgatttactt tttcctttag
    12361 acatactaaa aagggagaca gttcccttat ttaattataa ggtaacttcg ttaactacta
    12421 agaatcaatt taaaatgaag tttacgatcg tctaaaataa attgataata atcttcgatc
    12481 ttcttttgaa aatatttata ataatcttaa aacaaaaaat ctccaagatc tacaaattct
    12541 ttattagttg aattataaat aataattttg agttaggaat ttaactattt atatgatgtt
    12601 tcattgattt agcaactgaa aataaattat ttgtacatat caataattaa atcttgcttg
    12661 gatttattat aaaagtttga aatctaaagt tataagttat tgctagttca taacagaaga
    12721 aaatacgata cctgattcta ttacaaatgc aaaagattgt taaaaaagta aatatgctag
    12781 aatcatttta taaaactctt tttcacaatg aatgaccttc ttgttacatg agataaaaat
    12841 caatatgagt atgctaagtg ctaccccgat atcctataat agaatgactc aaattcgcag
    12901 ttttttgttg caggattggg aatgtctttt tataattgtt aaccttttca tctgtgataa
    12961 gagcatgaac cataaatata tgtgtcctat ttgtaaaaag aaattgattg aacattcaga
    13021 gttgcaggac aaaatatgca aaattattgc aataaagcaa ttgcaaataa ctctccaggt
    13081 tttgatgttc agtttagacc tttcttcgaa gattaatcac tttttttttc aatcttccct
    13141 ggtatctcaa tgccattata actatggagg caaatgaaga gctaggaatt aacatatgaa
    13201 ttttattaag caaaatgata tttattttgc atattattat cataaatagt aattcgttgg
    13261 aaaatggaaa tatagtaagg ataccaataa ataaagagtc tagagaaaaa atagaaggaa
    13321 atttaaatat tcctaaatcc tcaaaaagtt tagtaatctt tgctcatgga agtggtagta
    13381 gcagatctag tcctaggaat caatatgtat ctcgagtttt aaataatgat ggtgttgcga
    13441 ctctattaat agatctttta acagagagag aagaaaaaac agatgttgtt acaaaagaat
    13501 atagatttaa tatttcactt ttgggtgata gattaattgc cgtcacagac tggatcttaa
    13561 atcaagataa tcacttgaat accttcaata aaaaattggg ttattttggt gcaagtacag
    13621 gatcagcagc agctttaatt gctgctgaca aaagatctga taatataaat gctatagttt
    13681 caagaggggg gagagtagat ctcgctttaa aatatacttc attaagggaa atcaaatgcc
    13741 caactttgtt tttagttggg gagaatgatc atcctgtcat tgaatggaat cgacaagtaa
    13801 tagagaaaca attgataaat gtaggcaaga aaaaaataat tattattcct caagcaagcc
    13861 atttatttga ggaaccaggg aaattggaag aggtagcaaa atatgccagt ggatggttta
    13921 gatgctattt tcaaataaag gaaaaaactg ataataataa aactaaaaat aataattatt
    13981 aatttggaac atttttatgg atatatttat tcattttatt ataaaattaa gaatattaaa
    14041 attgaatcta gttgtacatt aaagttataa tagaaaaagt gatctagaaa gacaaatact
    14101 ttcgatgtgt ttccagagtt ttattgaata gattgcaaat ttcaaactca taactaatta
    14161 taattttaat tgatattata ttcttcaatc agtaaatata taaatatact aataagagga
    14221 attaatgtta taaattgttt ttgtagttac atctttttta attttataat tacgtaatca
    14281 attatttttt ctttggataa tttgtccatc tttgatatgg acttgaataa tatatattca
    14341 agaaatgaac catattcaga agtaattctt ttaagtaaaa tctttctatt ccttatatag
    14401 tactctgata gaggattgta tactaacgga ccaatattta aaatctcaat aatcttaaat
    14461 ttttcatgtt ctacaatatc taaaatatca tttagcaaat aatgttctga tgcccacgta
    14521 aaagaaagaa ttcctagttt aactaaattt tggatcggac ttaacaaatt cgtgataact
    14581 ggtattgcaa taacaaaaat tccatcctgt tttaatatat atcttgcttc tttaatgaat
    14641 tcaattaatg gcctaaagtg ttgagcagat tctaaagcta ttatcctatc aatcgatagt
    14701 ttattaaatg gtaacttaat agctgtacag ttaaatagag aaatatcctt ttcgaatttt
    14761 ttatttgaat ttttttgatc aaaataattc ttctttagtc tattggcaaa aataagatgg
    14821 tcgtgattaa tattaataca ataaatttct aataatgggt attcattttt ccaaaatatt
    14881 gcaggagaag agagaccgct tccaacatct acaagggttt tagctgattc aagttctgct
    14941 aatttcccta tcattttaca gagatgctct tgtgcttcta ttggattttt aattccatct
    15001 gaccaatatc caaaattaag catatagctg cctgttgtaa tagacatgag cggcgataag
    15061 gaactatata aattaatgac atcacgttga ttacgtctaa ttgcccataa tagagcatta
    15121 attggattaa ttaatatcac aagaatacac aaaattcaaa caaacaaaac taatatttgt
    15181 ttttgtatat aaggacattc tttatgaaca ttcccgcttt ttttagtaaa attcgtattt
    15241 ttaagattag gttattaaac gtcattcttt gtaaaggaaa gtagtatcat ttttttgtat
    15301 aatttcaaaa atagtatatc agtatattac atgcttaata acttaatgat tttgaatcaa
    15361 taacgtataa caaaatgtag taagaatatc tcaacctagg taatactatt gtaatggaaa
    15421 ttaacaaaag aattgttata atatctaata aaagtaagag ctaagaaaga attaccagaa
    15481 tatggtctat ctatggagtt tcaattaatg aacaattaat ctaaaagatt tctcattaga
    15541 aaaattatag gctcaggtta tgactaaacc agatcttgta catgagtggg tctcatagga
    15601 aacttagaag gtcacttgat gattgccacc tataagccaa gaaataaaaa aatattctct
    15661 taataatagt taacttcaaa acatattaat tgttataaat taaaaatttt aatataacct
    15721 atttctaatc taaaaatata ttagatgtat gaagagatac atgaattact ttagatcagg
    15781 taatggtcta ctaatgagat taatgggtta tgttctaatt acatttgtta ttccctttgt
    15841 cattaatatg atcaaaaaga aatttgatga ccaaaagcgt cttttatgta ataaatgtca
    15901 tggaaaatta gaagtaattg atacgagtaa gttttattgc aaaaaatgca aaataattag
    15961 atttgatcac aaataatgat gcaaatatgg aaaaaataaa agcgttaata ttccctaatt
    16021 gacctctaaa gaatttattt ataaaattag aaaaaactga aaagtttgta agacgtaaat
    16081 ggttattttt taatctaaaa gactggttat acaatattaa caaattaata tagtttaaat
    16141 cggtctctgt taagctaagc agtaagagtt atagctatgt acgctctatt tctaatgtct
    16201 gtgtatgtga ttatttaaaa ggtacagaac acatacagtt attgtaatgt aaccaaaaac
    16261 taaaatttat tcaatgtatt atttgaattc atgttaagta aacagaacca agttccaata
    16321 ttcatcaaat gtatcttcat aaatatataa aaaattttgt attagtatag tttattttat
    16381 tggattttat tctactgttt tgatagcaga actgtttatg ggtactaaag ccttttcttt
    16441 taatacttta ttgagtatac ttaaatcaaa tatattagat agatcaggat ttgtatctcc
    16501 taggaatcct aatttaaaag catcattagc tgatttataa agtgactgtt gtatagggtc
    16561 ataagtaaat tctaatcttg tcagtgattg ttgtaataca tcttctggta ttgtctgtcc
    16621 agtaatcttt tttagttccg tattaaaagt cttaacagct tcttctttat tgttatttat
    16681 ccattgtgtt ttgtctatat gagctttgag taaattttca ataacatctg gattttgttt
    16741 taggtagtct gttcttacaa gaatcaaccc agtaacaaat ttaccatcag cccataaatc
    16801 acgttcatcc aaaaataatt tgccatttgc atctttaatc aaacgttctc cccatggctc
    16861 aggaacccat gctccatcaa tatctttttt caaaaataat gttaaaatat ctgaattctt
    16921 tgctggtaaa acatgtacat tcccttgatt ttcaactgtc ttataaccat ggtctaataa
    16981 ataatttctc aaagcaacat cttgagtatt acctaattga ggtgatgcaa attttttatt
    17041 tgcaaaatcc gaggcagaat taattcctgt atcacctctt attacaaaga ctgctcctcc
    17101 actagtagca cctgaaatta tccttacatc ctttccttca gatatcacat agccattaat
    17161 agcaggattt gggccaacat atgtagcatc gatttgtttt gcaaataatg cttctatagc
    17221 ggaaggacct gcattgaata taaaaggctt aatttctaca ttagtaccta atactttttg
    17281 aaaatctcca tttcctatac ctatcacagc ctgagcatga ttgatgttag gaaaatatcc
    17341 aatttttaag atctttttat cctctgtctg agaaaatgac tgttgggcta aggtgaaaat
    17401 tatcgctatt cccaaaataa gggatattat tatacttgta gtggatctat attttttatc
    17461 cattaggcta attagataaa gtcgttataa taatgctctt aaatgtgaaa aatttaacaa
    17521 gtatatgcga tcttttccaa catttaacat ataactaaag taatataacg taattaaatg
    17581 gttaattatt gaagaaaacg atattgaata aagatattga atttatcctg caggagaact
    17641 aagttcatat attttttttc tagcatcctt aggatctaaa atctcgaata tcactccctg
    17701 aaattttagc tcacttattg atgcttgtat agttcttctt ggtagttcgc taatacgcat
    17761 taattcagtc tgatcaatag gatggaaaga ttttattata aaataaataa attttactga
    17821 tgggccgcct aagatattcc taacatttaa agttgtatgc tgatcagaag atttttccac
    17881 tttcgaataa tcttttcttc tactccattc aattaaccca acttgaaaag ggtcaattaa
    17941 acctaaattt atcctttcac cgaattttga aattaaaatt atttgtaggg attcgatgtc
    18001 agtctcaaat ataaaattac tttgagaata ggttgatatt aaagaggaaa taagtttcca
    18061 attagtgttt ctattaaaaa tatttagatc aaatttttga tctatttcta ggattatctt
    18121 tgttttgcta agtttaaata attctcgaat tttttcgaaa atgttctcat tatatatatt
    18181 gttacctgaa ttttttttgt taaatttcca ttgtacttct aaatcaaaag aatttgaaat
    18241 gtcagcaatc ttttgattat cctcaaaact attattcata ttgagttcta cttctataat
    18301 attaattcca attgacaata gctcacgaag gtattcattt agagtattat ttgctagtgc
    18361 gtaatctgta attttactgt caattgcaac tgagatctta tgttcatggt aaaattttat
    18421 tcgtctttca agttctgcag gggagtataa taaaaaacat gattgaggta tttttactga
    18481 atcaacaaat gaaactatgt ttttaaaatt ctcaatatct aatggttgta atttatctat
    18541 aatggaggta ataccttcaa gacgcggttt ttcattatct gtacgatcta aagttaatat
    18601 tttgttaagc aaacactctt tgtataatta attagatatt aatgaattat taaacatgta
    18661 aaaaattatt attatgtgaa aaatttgcat ataatatgta tataattgga ataaacaata
    18721 taacataatg gtcttttact gttgtagcct attttttcta atttatcatc ttttataaca
    18781 tatagaaata atgaacatac cataatctaa aaaattgttt tgatatgaat tatatgacaa
    18841 acacccccac aatgataagg agataagcac aaacaaaaaa tttgttacaa ttgccaactt
    18901 gtatgcaaat tttacccata ataatagtat taagcatatt taagaccaat taaatataat
    18961 aaattgctaa taaagggata tgatgcagat caaaaatcaa tatcctagac aaaaagtatc
    19021 tttgagccta aagaatgtat ccaaaaaata tacacaaaga aattctgaaa caagtatcgt
    19081 cttagaaaat gtcaatattc aaattttaga cggtgagatt gttacaattc taggaaaatc
    19141 cgggtgtggt aaatccactc ttcttaattt aattgcagga tttgaaaatt cttacggtgg
    19201 agaaatttta ctcaacggaa aacctgtaaa agatataaac cctgaacgga taatgatgtt
    19261 tcaagaatct gctctttttc catggcttac tgctttccaa aatgttgaaa ttgcattaaa
    19321 aatggcaaaa gttcccaaaa atcaacgaga aaaagtagta aaacattatc tggatatcgt
    19381 aggtttgtct gattattcta attcttatat tcatcaactt tcaggaggaa tgaaacaaag
    19441 agtatccctc gctcgggcgt tatcattaaa tcctaaaatt ctacttatgg atgaaccatt
    19501 tgcagcgtta gatattacga ttaaaaaatc tttatataga gaacttttag atctacatga
    19561 aaaaactaaa aagactatac tttttgttac ccataatata aatgaggctg tcttactagg
    19621 agatagggtt attgtaatgt ctccacgact ctatggtata aagaaagaat atgttattga
    19681 tatccctaag gatcaaagaa gtgataatct ctcaataaag aatataatta atgagattac
    19741 agctgatctt tacgatacca cttaatatat gaagtgtaag gtctgcaaag tttggaaaca
    19801 cagataaaaa gcattaagaa aaaggtaagc ttaagcaaaa aaatagtaaa taaaataata
    19861 aacaaagata cattaagttt agttattttc atcagtgtat tcttagcatt atggcaatta
    19921 gtttacatgt ccgaaatatt gccaaaatta tctctcccct cgcctcttgc cgtaggccaa
    19981 acaattaccg aattattatt ggattttacc ttaattaaag gtacggcttt cacattatgg
    20041 agattgtttt tagggtttat gctatctctt actcttggat tgatcacagg attgctcatg
    20101 attaaatttc aacaatttgg aaaaaccatg agttcatttg ccgtaggact acaatccttt
    20161 ccgagtattg catggattcc atttgcaata ttattgatag gattcaatga ttttggaata
    20221 ctttttgtgg ttgtaatgag ttctgttttt tctgtaatgc tctcaactta tagtggactt
    20281 aggaatgttc caccaatata tattcgcgcg gccagaaata tgggtgcaaa aggcttttca
    20341 ctctttagat atgtattgat tccagctgca actcctacgt taataatggg aataaggcag
    20401 gcctggtcgt ttgcttggca tgctctagta ggagctgaaa tgttaattac gacccttgta
    20461 ggattaggtt acattctttc agtaggaaga gaattctcta atatgagtca aataattgct
    20521 acaatgatag taatttttac aataggtttg atatttgaca gagtggtttt tacaaaaatt
    20581 gaagaaaaaa tacgagatag gtggggctta aaccagcaaa aaattgagca atagtatttg
    20641 taaatatttt tctaataaaa tttcatttta accaataaaa cactcgataa ttttaactac
    20701 tatatttgtt aatcttgatg ttgaacttgg cattttcttc tttaatactg tttatttttg
    20761 ttgatattgt ataaaaaaca gtaagctgga tttccgaatg aaaaatagag agtatcaaat
    20821 gcagatataa acagctatta atgtcttatc tttctatttt ttcaatatct ttatattttt
    20881 taggctctgt taatttaaga tatgttcacc ttcttctctc aacgactaat ggatacttct
    20941 tgttggatat gatatcaatg tacatagaaa ccagaattgt aaccagttct atacatgaaa
    21001 tagattacct tcgttttgat tttttcagtt aaaaatcgaa gatatgaaat tggaatatta
    21061 tttctaattt attgctattc tatcaatgta tatcttattt tttttgcatc ttggacattg
    21121 agtaaccttg aatacatttt ttaaaatagt ggcaaaccaa aaacatgaat cacataatac
    21181 aaatctaaaa ttttttttat tataatagtg tttatttact ggtaaataag attcaacaac
    21241 tcttacattt ttatcttcaa gatgttcttc tacccattgt gtatcaacaa gaacttctgg
    21301 atgaacatat
//
LOCUS       DASVEV010000006        10346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_391645, whole genome shotgun sequence.
ACCESSION   DASVEV010000006 DASVEV010000000
VERSION     DASVEV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391645"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..297)
                     /locus_tag="VF222_00270"
     CDS             complement(112..297)
                     /locus_tag="VF222_00270"
                     /inference="COORDINATES: protein motif:HMM:NF002316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HEX7140891.1"
                     /translation="MRNSILFTGMAARKNTSRKKRLIRKIKQNRPVPAWVIIKTHRQV
                     RSNPKRRLWRRSDVNVG"
     gene            complement(535..663)
                     /locus_tag="VF222_00275"
     CDS             complement(535..663)
                     /locus_tag="VF222_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140892.1"
                     /translation="MEFKASDGFEEVHHTCRSCKTHFNHLDGDTYDHCEECNYNNK"
     gene            complement(854..2266)
                     /locus_tag="VF222_00280"
     CDS             complement(854..2266)
                     /locus_tag="VF222_00280"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HEX7140893.1"
                     /translation="MQKLKPDLIEYISILKKDVKINNYDTISRWDNKIISHRNSKKNI
                     WIEAYGCSANIADSEIIAGTLNNHGYNIVNCIDESDLNIIVTCSVKDSTEHKMLHRIK
                     KLTGDNKPLIVAGCLTKTERKKIEKINPNVSLLGPHSLDKSVEAVNLTFSNNKIVFLD
                     DSSAEKVNLQRIRVNKSISIVEIASGCLSNCSFCQTKISKGNLKSYRPGSIISQIKND
                     VKDGCNEIWLTSTDNGCYGKDINSNLVELLKLCTQLEGNYKLRIGMLNPMYLSEILHD
                     LISVYKNEDKIFKFLHIPVQSGSNKILKHMYRGHDINIFKKAVYEFRKSIPEITIATD
                     VIVGFPNESEEDFKLTMDLIRESEPDIINISKYSQRSGTVSSRLKNISSDQIKLRSTI
                     LHSLSRDISRRRNTLWKGWIGDVYINEIKNTLVQGRNYAYKSVIINNSDTLNLRIGQI
                     IKVKVVDSSQYSLFAEFINF"
     gene            complement(2351..3223)
                     /locus_tag="VF222_00285"
     CDS             complement(2351..3223)
                     /locus_tag="VF222_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX7140894.1"
                     /translation="MYNKRKISIRERLADDNDIIILPGVYDALTAKIAEDVGFETAFQ
                     TGYGTSASLLGMPDFGFLNAGETLENAKRIIYSVNIPILVDIDTGYGNPLNVWKIVKD
                     LERIGAKGIFLEDQVWPKRCGHMAGKTVIPKEEYILKLQAAIDAREDSEFIIVARTDS
                     LAQFGIEEAIERGKEYKRIGADVIFIEAPKTIDQMELIAKEIKAPLLANMIEEGITPN
                     LTANQLKKMGFKMVVFPLSALYSATFAIKQTLQTLKKTGTTKELKDKMITFQEFNDLV
                     NLSAYNKLEEKYSS"
     gene            complement(3268..3426)
                     /locus_tag="VF222_00290"
     CDS             complement(3268..3426)
                     /locus_tag="VF222_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140895.1"
                     /translation="MKCPVCGNIMIWLNGSISHDPPIKYYSCRECMIGVSKYSETNYD
                     IYEIKNNK"
     gene            3976..4464
                     /locus_tag="VF222_00295"
     CDS             3976..4464
                     /locus_tag="VF222_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7140896.1"
                     /translation="MISHWFSRVNSVIPRGFSRQYILNLLKEKPMSGKEIIDCAIYES
                     GGKWKPSPGLIYPMLGRLLEEGLISETENGKYKVTHKGVGIAEDIQSIQKIFQKQLDV
                     MFRFGSIGRFMAMDLIDRVSSIGSTLSANLDKMTEQEREKYKEFLITELKKIEEQEKE
                     LR"
     gene            4536..4895
                     /locus_tag="VF222_00300"
     CDS             4536..4895
                     /locus_tag="VF222_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140897.1"
                     /translation="MISELLECIDKLTEKINQIENDIQNASSIVIKEYQSTSEKIHEN
                     QSYFLSGIMYGIPGKSYLLTSKGIEVLGEETIEIGVFINDVINYANNPTRKIEVLKDL
                     VTHLQKFLESMNNIPPK"
     gene            complement(4918..5184)
                     /locus_tag="VF222_00305"
     CDS             complement(4918..5184)
                     /locus_tag="VF222_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140898.1"
                     /translation="MKEDEAWNLIRPVCRELDELVNLGQVKFLSGFHSEREGSYKIYL
                     KCSKIHLSSRGIFDKIGDIEYQNNMLRIGFSSNKIPLNLVINMT"
     gene            complement(5257..5709)
                     /locus_tag="VF222_00310"
     CDS             complement(5257..5709)
                     /locus_tag="VF222_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140899.1"
                     /translation="MIQKDSNFFIVIGFFLTIFFLGSAFIVDSFGVMDQNLFQSTTGG
                     SLDVNITSPEVINKDKEVKFKINFIEPTTDKIQVHVDYDFQVLDNGKEIFSAAKQINQ
                     PLLHTAEGSVTIPFMFSSSGVYSIKIPVLGINFIPINPEYAEFKFVVK"
     gene            complement(6327..6761)
                     /locus_tag="VF222_00315"
     CDS             complement(6327..6761)
                     /locus_tag="VF222_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX7140900.1"
                     /translation="MNDIFSSNDEYIQVEISNIGYADHLGLEGFLILKSMEGKEFPIN
                     AFSGEVAKHILRFREGDKNTIPTIYNLVEEIAVMQDLLLMEVRIYMSGSVLRANLYFR
                     GRKDTLILRNYRASDAIALASYYDVPIKVRKNLFEDELQTTE"
     gene            6867..7700
                     /locus_tag="VF222_00320"
     CDS             6867..7700
                     /locus_tag="VF222_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140901.1"
                     /translation="MKIKEDILLNKGLRIIIFSVSSLVFSYIFYSKFTNPNFNDIIMF
                     TPVLLNISIGILIITFISSIAICFGLWNLYVSNELAKKNLKYFISLPFKEKRYTLIMI
                     LSGIIYGIVFSFLSQILVFTDNGSNSFEDIKFSPSIKIIPCCNTIGYVPITYIYLAEY
                     FFIFLIPINIALVFIVSFFVGFNISINIFMIKKLKENYKNNKNNKSKVIGGIGATCGL
                     FIGCPTCAGSIIATILGYTAGTTTISILASFQTVFIVISIPALMIAPFLIAKTVKNQI
                     I"
     gene            7878..8048
                     /locus_tag="VF222_00325"
     CDS             7878..8048
                     /locus_tag="VF222_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140902.1"
                     /translation="MKDESIERVLRSFIDEKSLIKLLRVLDEENKEKETQEIDKKFRK
                     IPHKDIINPFYN"
     gene            8132..9316
                     /gene="mpgS"
                     /locus_tag="VF222_00330"
     CDS             8132..9316
                     /gene="mpgS"
                     /locus_tag="VF222_00330"
                     /EC_number="2.4.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050504 - mannosyl-3-phosphoglycerate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX7140903.1"
                     /translation="MKLDLPRFTERFGSISLHGVQRVFELDSGSEKGKKVSENITGVG
                     NEQISEIESKMAIVIPIKNERLKLLEGVLSGIPHDCMTIIVSNSPRQPVDRYKLEKES
                     LQQFNRFANKNALIIHQRDEGLAETLREVNYNDLIGPDHLIRNGKAEGMVLGMLLAKM
                     ANKEYVGFIDGDNYVPGAVNEYVKIFASGIAMSDTPYKMIRVSWIYKPKISESGIYFS
                     KWGRVSEITNHYLNSLISYYTGFETEVMRTGNSGEHCMSLKLAELLHFSSGFSVETYE
                     IVNLLEQFGGILPTENQDAMDKGVEVMQVETRNPHFHEDKGEAHLKEMINGSLGCIYH
                     SRICHPKLQEKILEELKNKSTLDNNEAPPILEKINPFKDVDIKTFDKKLHDKATSLIH
                     WN"
     gene            complement(9383..>10346)
                     /locus_tag="VF222_00335"
     CDS             complement(9383..>10346)
                     /locus_tag="VF222_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HEX7140904.1"
                     /translation="RKITVEDPNLIIKINEESGETLMAGMGVLHLEIATSLLQEAGLS
                     ITTSQPLINYRETIRSKGGPIMSKSPNRHNKIFMRVEPLREDIIEMIKTGHIKEDMDK
                     KELAKILREKGWNADEARNVSAIDPTGNILIDETKGVQFIQESMDSIRSGFDDVVHSG
                     ALAHETLRGVKFVLNHFVPHEDPAHRGLAQLMPATRRSMLGSMLIADPVLLEPILGIE
                     VKCPQEQIGIVAGILSGKRGKLLNVDQKGVIAIMQGEVPASETFDLSEVMRGGTAGKA
                     MWSTYFKSWQAVPQSVIRTLIADVRKRKGLNPEPPTPDEFIDKE"
BASE COUNT         3648 a         1534 c         1483 g         3681 t
ORIGIN      
        1 ttactctatc agttcgtcga tattgaggag ttaaccaagc ctttcctaga tttatggtat
       61 atacatgagt tgtatcctca ttagattttg acatattcat aatcaccatt cttatcctac
      121 gttgacatcg gatcttctcc aaagtctacg ttttggattt gatcgaactt gtctatgcgt
      181 cttaataata acccatgcag gcacaggcct attctgttta attttcctaa ttaatctttt
      241 ttttctagat gtattttttc gggcggccat cccagtaaac aggatgctat ttctcacttt
      301 taagtgttga tatgtgttat ttgagctata aagaattgaa ctgtataaaa aataatataa
      361 aagtaaatcg gttttattat tatggtagag ctgtgtagta tgtgttttga attcttcaag
      421 gcaaataaaa atgtaaaaga tatccagaat attaaaattg ttgaaaagaa tcgatgtaat
      481 ggattatacc ataaaaaact aatagatagt tttaaaacat gatctaaact taaattattt
      541 attgttataa ttacattctt cacaatgatc atacgtatca ccatcaagat gattaaaatg
      601 agttttacat gatctacacg tatgatggac ttcttcaaat ccatcagaag ctttaaactc
      661 caagtattct aaattgatac ttttacattt gatacatctg caattgcaaa acatacacaa
      721 taaataactt tcatgtataa attagttttt atttttatat aatagaaaaa attatggatt
      781 agatatcact tcgtaatgga cacatataaa acaaggtaat attattcatt aacaaatcta
      841 tcctataatc ttattagaaa tttatgaatt ctgcaaaaag tgaatactga gaagagtcca
      901 ctaccttgac cttaattatt tgtccaatac gtaagtttaa tgtatcagag ttatttataa
      961 tcactgattt ataggcataa tttcttccct gaactaaagt attttttatt tcattaatat
     1021 atacatcgcc aatccaacct ttccagagag tatttcttct tcttgagata tcacgagaaa
     1081 gactatgtaa aattgtagaa cggagtttta tttgatcaga agatatattc tttaaccttg
     1141 aagatactgt acctgatctt tgactatatt ttgatatatt tatgatatct ggctcagact
     1201 ctctaattaa atccattgtc aatttaaagt cctcctcaga ctcatttgga aaacctacta
     1261 taacatcagt tgctattgta atttcaggaa tagattttcg aaactcgtat acagcctttt
     1321 taaaaatgtt aatatcatga cctctataca tatgttttaa aattttatta ctaccacttt
     1381 gaactggtat atgtaagaat ttaaatattt tgtcttcatt tttatatact gaaataagat
     1441 catgtaatat ctcagataaa tacattggat taagcattcc aattcgaagt ttataatttc
     1501 cttctaattg agtacataat tttaacaatt caactaaatt tgagtttata tctttgccat
     1561 aacagccgtt gtcagttgac gttaaccata tttcattaca accatcttta acatcatttt
     1621 ttatttggct aattattgat ccaggtcgat aacttttaag attacccttt gaaattttgg
     1681 tctgacaaaa ggaacaatta cttaaacaac ctgatgcgat ctctacaata ctaatagatt
     1741 tatttactct tattctttgt aagtttactt tctcagcaga tgagtcatca agaaatacaa
     1801 ttttattatt tgagaatgtg agattaaccg cttcaactga tttatctaaa gaatgtggac
     1861 ctaataaact gacgtttgga ttaatctttt caattttttt tcgttctgtt tttgtaagac
     1921 atcctgctac tattaatggt ttattgtctc cagttaattt cttaatacgg tgaagcattt
     1981 tatgttcagt agaatcttta actgaacatg ttactattat atttagatct gattcatcaa
     2041 tacaattcac tatattatac ccatgattat ttaatgtacc agctataatt tccgagtctg
     2101 caatattggc agagcatcca tatgcttcaa tccatatatt ttttttacta tttctatgag
     2161 atattatttt attgtcccat ctagaaatag tatcataatt atttattttg acatcctttt
     2221 tcaaaattga gatatattca ataagatccg gttttagttt ttgcattagt ggctaaatta
     2281 atactaaatc aatattctat ataattatta agattattga accaaaacaa tggcttagaa
     2341 aaaaataggt ctacgaagag tatttttctt ccaatttatt atatgcagat aagttgacaa
     2401 ggtcattgaa ttcttgaaaa gtaatcattt tgtctttcaa ttctttagtt gtacctgttt
     2461 tctttaaggt ttgaagagtt tgttttattg caaaagtagc tgaatataat gcagataatg
     2521 gaaaaacgac cattttaaaa cccatttttt ttagttggtt agcagtaaga ttaggagtta
     2581 ttccttcctc aatcatattt gctaatagag gagccttaat ttcttttgca atcaattcca
     2641 tttgatcaat agtttttggt gcttctataa aaataacatc tgctccaatt cttttgtatt
     2701 cttttcctct ttctattgct tcttcaattc caaattgtgc aagagagtct gttcgtgcga
     2761 ctatgataaa ttcactatct tctcttgcat ctattgcggc ttgtaattta agaatatatt
     2821 cctcctttgg aataacagtt ttgccagcca tatgaccaca tcttttaggc cacacttgat
     2881 cttctaaaaa aattcctttt gcaccgattc tttctaaatc tttaacaatt ttccaaacgt
     2941 ttaaaggatt tccgtatcct gtatctatat caaccagaat cggaatattt acagaataga
     3001 taattctttt tgcattttct aaagtttccc ctgcattcaa gaatccaaaa tctggcattc
     3061 ctaacagaga cgctgatgta ccataccctg tttggaatgc tgtttcaaat cctacatcct
     3121 cggcaatttt agcggttaaa gcatcatata cccctgggag gattataata tcattatcat
     3181 ccgctagacg ttcccttata gagatctttc ttttattata catcactact taaacagatt
     3241 tattaattaa taaagagatt taaaaaatta tttgttattt tttatctcat agatatcata
     3301 attagtttca gaatatttag aaacgcctat catacattct ctgcaagaat aatattttat
     3361 cggaggatca tgactgatag atccatttag ccatatcata atgtttccgc acacaggaca
     3421 tttcatagtc attattaaaa gatttatgaa aattcaataa tatattttaa tacttaacaa
     3481 aaaatcatat aaaataaaca gttttttttc taccttatct tgtcataatg catctaaatc
     3541 tgatctcctt gtcctttagc attgtcgttc tttgttggtg ttagagaata acctgtaaca
     3601 cccaaaaaac tatattcttt ttatcattat tgtacttgta gtatattaca caaactatat
     3661 tcgaaatttg aaaaagttaa caaaatgggt gttatgcggc aaatctaaca cccatttcta
     3721 aaaagctgca ccagggattc agttgaaatt ttagcttaat tatatatcat tctttttttt
     3781 gaagaaatct actgaatggt taaagaaagc ccaatatcta aacttttatg gacggggatg
     3841 aatagcgaaa ttttccagct aatatctcat agttttttta tctgtcgaat tatatcacct
     3901 acctaaatca gtccattatt cagcagaact tattgaaaag aaatattaat aaatcgctag
     3961 tgatattatg aatgtatgat ttcccattgg ttttcaagag taaatagtgt tatccccaga
     4021 ggattctcta gacagtatat cttaaaccta ttaaaagaaa aaccgatgag tggcaaagaa
     4081 attatagatt gtgcaattta tgaaagcgga ggaaaatgga aaccatctcc aggtcttatt
     4141 tatcctatgc taggcagatt attagaggaa ggattaattt cagaaacaga aaatggaaaa
     4201 tacaaggtta cacacaaagg tgttggaatt gcagaagata ttcaatcaat acaaaagata
     4261 ttccaaaaac aattggatgt tatgtttagg tttggaagta taggaaggtt tatggctatg
     4321 gatctaattg acagagtatc gtctatagga tctacattaa gtgccaattt ggataaaatg
     4381 accgaacagg aacgtgaaaa gtataaagaa tttttaatta cagaattaaa aaagatcgaa
     4441 gagcaagaaa aggaattaag ataaaaattc tattttcatt agatagcata gggaaaatta
     4501 tatatgctta aagaagatca atatcaagga gtactgtgat ttcggagctt cttgagtgta
     4561 ttgataagtt aacagaaaag attaatcaga ttgaaaatga tattcaaaat gctagtagta
     4621 tagtaataaa agaatatcaa agcactagcg aaaaaattca tgaaaatcaa agctattttt
     4681 tatcaggtat tatgtatgga ataccaggaa aatcctattt actaacttct aaaggaatcg
     4741 aggtattagg ggaagaaaca attgaaattg gtgttttcat caatgatgtt ataaattatg
     4801 caaataaccc gactaggaaa atagaggttt taaaagacct tgtcacccat ttacaaaaat
     4861 ttttggaaag tatgaataac attcctccta aataacaact ttgtaaggtt tgaaagtcta
     4921 agtcatatta atcacaagat ttaacggtat tttattacta ctaaacccta tccggagcat
     4981 attattttga tattcaatat ctccaatttt atcaaaaatt cctcttgaag aaagatgaat
     5041 tttgctgcac ttaagataaa tcttatagga accctctctt tcactatgaa agccagagag
     5101 gaattttact tgtcctagat taactaattc atccaattct cggcagacag gtcttattaa
     5161 attccaagct tcgtcttctt tcaaaacaat aaagtataga aactttacaa aataaaaaat
     5221 gttacgttat acaaaaaatt gataaagata attcaattat tttacaacaa atttgaattc
     5281 agcatattcg gggtttatag gaataaaatt tatccctaaa acaggtatct taattgaata
     5341 aaccccagat gatgaaaaca taaatggaat agtaacagaa ccttcggctg tatggagaag
     5401 aggttgatta atttgttttg ctgcagaaaa tatttctttt ccattatcca agacctgaaa
     5461 atcataatca acatgaacct gaattttatc ggtagtaggt tcaataaaat tgatcttaaa
     5521 ttttacctct ttatctttat taataacttc aggagaagta atatttacat ccaaggatcc
     5581 accggtagtt gattgaaaca agttttgatc cataacacca aaagaatcta caataaaagc
     5641 gcttcctaaa aagaaaattg ttaaaaaaaa tcctattact ataaaaaaat ttgaatcttt
     5701 ttgtatcata atatgtctac aagtaactga gtatatgtat ttattcatta aaatattaaa
     5761 aaaatcacaa ggaaattatc ttacattact attgttgatt cgattatcaa catgctaaaa
     5821 agtcaaacta ttgtaattaa tacaaagcca taaattgtat tttattaaaa taactatgaa
     5881 ttgcaaatag aaaagatatt acaaacttaa ttaatgcttc tatgaatagt cacacaaatc
     5941 aaaagtaaga aatatccgct taaatctaca agataaaaag agattattaa agttacggat
     6001 aaaaaatatg ggcgattttt aggcaaattc ttaatctcct ttctcttata atttaaaagt
     6061 gtacatctat atcaattgat tttttcttgc taagtattta tattttataa ttttatggtt
     6121 gattttttga ataatatgat aaaataaata attcaaattt ttatttttct atgtagaaag
     6181 aagaaataat acattaataa tctttatctg tttatagaaa ttataataaa atattatatg
     6241 ttagttcatt tcaaattcca attttatatt taaattttga ataataaaat gaacagaata
     6301 aagaaattca taaacttttg attggcttat tctgtcgttt gcaattcatc ttcaaataag
     6361 ttctttctca cttttattgg aacgtcataa tatgatgcta atgcaatcgc atcagatgcc
     6421 ctgtaatttc gaagaataag tgtatccttt cttcctctaa aatacaagtt agcacgtaag
     6481 actgatccgc tcatatatat tctgacttcc attaataata gatcttgcat tacagctatt
     6541 tcttcaacta gattatatat agtaggaatt gtgttcttgt ctccttctct gaaccgaagt
     6601 atatgttttg caacttcacc cgaaaatgca ttaataggaa attcctttcc ttccatagat
     6661 ttcaaaatta aaaaaccttc taatcccaaa tgatcggcgt acccaatatt actgatctcg
     6721 acttgaatgt attcatcatt tgatgaaaaa atatcgttca tctctatcaa tattaggata
     6781 aactacgaga taaatttttc atgtatttaa tatataataa taaaatttat gtttggtggt
     6841 ttattttagt atagtaattc actactatga agataaaaga ggatatttta ttgaataaag
     6901 ggttaagaat aataatcttt agtgtttcat cattagtatt ctcttatatt ttttattcaa
     6961 aatttactaa tccaaatttt aatgatataa ttatgtttac accagtgtta ttgaacatat
     7021 caataggaat tctaataata acgtttattt catcaatagc tatttgtttc ggtttgtgga
     7081 atctttatgt ttcaaatgaa ctagcaaaaa aaaatttaaa atatttcatt tccttaccat
     7141 ttaaagaaaa aagatatact ttaataatga tattatctgg aattatttat ggtattgttt
     7201 ttagttttct ttctcagata cttgtcttta ctgataatgg atcaaattca tttgaagata
     7261 ttaaatttag tccatcaata aaaataatac catgttgtaa cacaatagga tatgtaccta
     7321 taacatatat ctatttagct gaatattttt ttatctttct aattccaatt aatatagcat
     7381 tagtattcat tgtttctttc tttgtaggtt ttaatatttc tataaatatt tttatgataa
     7441 aaaaattaaa agaaaattat aaaaataata agaataataa atcaaaagtt ataggaggta
     7501 taggtgcaac atgtggatta tttattggat gtcccacctg tgcaggtagt ataatagcaa
     7561 caattttggg atatactgct ggaacgacca ctatatcaat attggcatca tttcaaaccg
     7621 tatttattgt tataagtatt cctgcattaa tgattgcacc atttttaatt gctaaaactg
     7681 ttaaaaatca aatcatttaa taattattta aaatagttta ctaggtttta tcttaataaa
     7741 atgttgtcct cctaaattaa ttaagaacaa aatatctatt aaaaaactag tgtaattttg
     7801 ttttaaaaaa aaagggaaaa ttatagggta tcagattgaa tatcagttaa ataaaatagt
     7861 agcaattcgt taacaaattg aaggatgaat caatcgagag agtattaaga tcattcattg
     7921 atgaaaaatc tttaattaaa ttacttaggg tacttgatga agaaaataag gaaaaagaaa
     7981 ctcaagaaat tgataaaaag tttagaaaga taccacataa agacataata aatcccttct
     8041 acaattaaaa tttaatcaat ataattaaat ttgatttaaa tgtatgtgag tatcgtttta
     8101 atttggattt tacgataaag gagagaaata aatgaaatta gatttacctc gatttactga
     8161 aagatttgga tcaataagtc ttcatggtgt ccagcgagtc tttgagcttg attctggctc
     8221 agaaaaagga aaaaaagtgt cagaaaatat tacaggtgtc ggaaatgaac aaatttcaga
     8281 gatagaaagt aagatggcca tagtgattcc gataaaaaat gaaaggttga agctcttaga
     8341 aggtgtgtta agtggaattc ctcatgattg tatgacgatt atagtctcaa atagtcctcg
     8401 gcaaccagtt gatagataca aattagagaa agagtcactt caacaattta atcgatttgc
     8461 taacaaaaat gccttaatta ttcatcagag ggatgaaggt cttgctgaga ccttacgaga
     8521 agttaattac aatgatttga taggtcctga tcatcttatt agaaacggca aagctgaagg
     8581 aatggtacta ggaatgcttc ttgctaaaat ggctaacaaa gaatatgtcg gtttcattga
     8641 tggagataat tatgtacctg gtgctgtcaa tgaatatgta aaaatttttg catcaggaat
     8701 tgcaatgtct gatactcctt ataagatgat aagagtttcc tggatttata aacccaagat
     8761 ttctgaaagc ggaatttatt tttcaaaatg gggaagagtg tctgaaatta ctaatcacta
     8821 tttaaattca cttatttcgt attatactgg atttgaaacc gaagttatgc gaactggaaa
     8881 ttcaggtgaa cattgtatgt ctctcaaact agcagaacta cttcattttt cttcaggatt
     8941 ctctgtagaa acttatgaaa ttgtgaattt acttgaacaa tttggtggaa ttttacctac
     9001 agaaaatcag gatgcaatgg ataaaggagt agaggtaatg caggtggaaa ccagaaatcc
     9061 ccactttcat gaggacaaag gagaagcaca tttgaaagag atgattaatg gatcgctagg
     9121 gtgtatatat catagtagaa tatgtcatcc aaagttacaa gaaaagatac ttgaagagct
     9181 aaaaaataaa tcaacattag ataataatga ggctccacca atattagaaa aaataaatcc
     9241 atttaaagac gtagatataa aaacatttga taaaaaatta catgacaaag caactagttt
     9301 gatacattgg aattaactaa attgtgtcaa attcgttaaa aacataatat agaaataacg
     9361 taaaaaaatg tgaaatttcc tattattctt tgtcaataaa ttcatcagga gttggaggtt
     9421 ctggattaag cccctttctt ttgcgtacgt cagcgataag tgttcttata acagactgag
     9481 ggaccgcttg ccaactttta aagtacgtac tccacattgc tttacctgct gtcccgccac
     9541 gcataacctc ggataaatca aaagtttcag aagctggaac ttcaccttgc ataatagcta
     9601 taacaccttt ttggtcaacg tttaataatt taccacgttt ccctgataaa attcctgcta
     9661 ctatacctat ttgctcttga ggacatttta cttcgattcc cagtataggt tccaaaagta
     9721 caggatctgc gatcaacata gaacccaaca ttgaccttcg tgttgccggc atcaattgtg
     9781 ctaaccctct atgagcaggg tcttcgtgcg gtacaaaatg gttaagaaca aatttaacac
     9841 ctcgtagagt ctcatgtgct agcgctcctg aatgcactac gtcatcaaat cctgatctga
     9901 tagagtccat tgattcttga ataaattgaa cacctttagt ttcatcgatc aatatgtttc
     9961 ctgtgggatc aatagcgcta acatttctcg cttcatcagc attccatccc ttttctctga
    10021 ggatttttgc taattccttt ttatccatat cttcctttat gtgtccggtt ttaatcattt
    10081 caataatatc ttccctaagt ggttcgactc tcatgaatat cttattatgt ctattaggag
    10141 atttactcat tatgggtcca cctttggaac gaattgtctc tctataattg attaaaggtt
    10201 gggaagtagt tatagataga cctgcttcct gtaacagtga cgtagcgatc tcaagatgta
    10261 atactcccat tccagccatt aatgtttctc cactttcttc atttattttg ataataagat
    10321 ttggatcttc aactgttatt ttacga
//
LOCUS       DASVEV010000007        11671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_3219, whole genome shotgun sequence.
ACCESSION   DASVEV010000007 DASVEV010000000
VERSION     DASVEV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3219"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF222_00340"
     CDS             complement(<1..300)
                     /locus_tag="VF222_00340"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7140905.1"
                     /translation="MLKNAPNGWKVLEDERNVYLNEYIQIYEDLLDINGFKKIYVRGK
                     RKNYSTVVPFISADKILVIKSYRHLVDSVQVEIPSGYIEDGESPKDAAIREFKEET"
     gene            complement(860..1357)
                     /locus_tag="VF222_00345"
     CDS             complement(860..1357)
                     /locus_tag="VF222_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7140906.1"
                     /translation="MKNYKNLKENSSPVNSFLIAIQKIYYNKGELSKFYIVGGIGLVI
                     NYFVSYFFFTYFHLDHIQSSIFGIIVSLSSNFMFNKIWTFQDRNMKYNILLRQYLKYF
                     IFNSVGVIIQLSIVYGFGKTNLDYGWVIIIAISIASILNYIVNKKFIFKNRKEVVDIR
                     NRFWN"
     gene            complement(1723..1866)
                     /locus_tag="VF222_00350"
     CDS             complement(1723..1866)
                     /locus_tag="VF222_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140907.1"
                     /translation="MQINYIESNNGKVPLILTGYKDKMDEFLQKYQLKIVEENITNIA
                     NSR"
     gene            2194..2403
                     /locus_tag="VF222_00355"
     CDS             2194..2403
                     /locus_tag="VF222_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140908.1"
                     /translation="MQKKNICTSCNEKFRTSEGLLKHAKEIHKQIIRKCNNCGKQFLN
                     EKERLHHMKEEKQREIDYRRHKNLK"
     gene            2607..2831
                     /locus_tag="VF222_00360"
     CDS             2607..2831
                     /locus_tag="VF222_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140909.1"
                     /translation="MRKILITASEKIINQIEKKFRHNFEIDFEYSDSSEGKIEVIKNN
                     RWVTICRFSSDESLDNIITMFNVNYEIKCR"
     gene            complement(2892..3158)
                     /locus_tag="VF222_00365"
     CDS             complement(2892..3158)
                     /locus_tag="VF222_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140910.1"
                     /translation="MVPLLISAIIGFAVSEITTHYWKRKHPSKVYFFDRRIHHGELGV
                     LLLCILSMGKISPVLSSFIIGCGLGLIKDDLQDKSKWFKFQKRR"
     gene            complement(3722..4243)
                     /locus_tag="VF222_00370"
     CDS             complement(3722..4243)
                     /locus_tag="VF222_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140911.1"
                     /translation="MKKQRDILLTMICFMIFFSLTTSDIDDDSNSSVDFKKDTIKLSS
                     QGNYSYSISPDIFQTTYSSNANDKVNSNSINLLENVVNYIITIEVVHISGDTIKRSLI
                     AQVNNAILILKDDDSDNDKIICNNFVNKFVSNIKLYERIGVFQPNSSSKILKDILILK
                     NSLCDLNNTNISK"
     gene            complement(4343..4792)
                     /locus_tag="VF222_00375"
     CDS             complement(4343..4792)
                     /locus_tag="VF222_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140912.1"
                     /translation="MDTIILEYLTSYLHFFSSILWWGLTFFVVVILGPVNRNGSYSLI
                     LPRIHKFILPISTVSITSGLALTFININFDPNRLFNSLWVYMLILGGLFSIPVYLVIL
                     FRSKKRTIKIQFSKKRNSKKTNFLPFLLFALLSSTIFIMIFVTKFFS"
     gene            6046..6489
                     /locus_tag="VF222_00380"
     CDS             6046..6489
                     /locus_tag="VF222_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140913.1"
                     /translation="MEYKLLIGNNFIFGNPKKIYYKDFHIFTIDEDIDGPYIDTELFD
                     TKGRKIVEIKKNNLLLCNTEFDKKALKRDHILILDKRGEIIIESRVFNKDTIIISGRF
                     IIHKVVLTITQNYILLPEGKRIMHSKFRSNNNSVTITENGIVSKK"
     gene            complement(6638..8185)
                     /locus_tag="VF222_00385"
     CDS             complement(6638..8185)
                     /locus_tag="VF222_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7140914.1"
                     /translation="MHQEKIDDDLRSIAKGNVLSDDWNKKIYSVDASEFAILPSTIIQ
                     VKDKEDISNICKYAYSKHLTITGRGAGTGLLGQSLTTGISLDFTKYMNKIIEFENDYV
                     IVQPGIVKAVLDKELIKRNKFLPPDPASSNFCTIGGMIANNSSGIHALGYGNTIDFLE
                     GIEIIYSNGIIESLFKNPHGINDSATIESDSNRPNKINQLFKLFSPDILQYISLKFPK
                     VTKNSCGYRIDTLIDNNKRYFPHKIFASSEGTLGITTQARFRILDLPLYKYVMVIGFE
                     NILKAVKSVPKILTFLPSSLELLDYSILSYENSISNPSNFKVNNNRGGTLLFVEFAGD
                     KLVNIEIQIKLCREKLSAYGEILEVTSDNISSERIWSARKNALNNVMKMTVGSRKPIS
                     LIEDTIVNPNYLYDYTLFLLNTYKKYKLDYIFYGHAGNGNLHTRPMIDTASNKYKEVI
                     GSLAKEVFSKVYKLSGSITAEHGDGISRTKYISQMYGNTIFELFKKIKFIFDPTNIMN
                     PGKKVIQ"
     gene            8679..8888
                     /locus_tag="VF222_00390"
     CDS             8679..8888
                     /locus_tag="VF222_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140915.1"
                     /translation="MRIGYNKDKRKVFDKYLYGCPCCHLSRYVILSEDIQDPAYHKYE
                     LYCHSCNVVFSSMHAVLPLEFNQEI"
     gene            complement(8949..9107)
                     /locus_tag="VF222_00395"
     CDS             complement(8949..9107)
                     /locus_tag="VF222_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140916.1"
                     /translation="MVSTRQFLGIIGIIMAVLGGIAWFFQQKLTAFMLWGGAALIMFK
                     LNKRTKKK"
     gene            complement(9196..10635)
                     /locus_tag="VF222_00400"
     CDS             complement(9196..10635)
                     /locus_tag="VF222_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7140917.1"
                     /translation="MPDLQNMDKSYLSKCKSIIALSQEIKTGNIKPTELVDYYLVAIA
                     RLNPKLNSFITVLNEQSLQEAERLENELKSGFYRGILHGIPFSVKDIIAVSNVRLTAG
                     SKIFLEHISKSDSTVIKRLKKAGAILLGTNNLNEFAAGITGKNFLFGNSKNPYSLDRI
                     SGGSSGGSAVAVAAGLIPFALGTDTGGSIRVPSSLCGIFGFKPTFDSIDNKGVYKLSP
                     SLDHIGIMGKNSIDLSIVFSTIRKKRRKFVGYINELEIQKLSLLNLKKILSQNKRTIS
                     IGVPCHYFLDYLENNVKYSFTNFIDLLKSDNLLSFIHVEINSEDFFRSWWIVRLFEAY
                     TIHKRGLKKKFSYYGPELRDQLLEGAKIKHKDYYESLNNIKKIKKQFLSLYSKFDFMV
                     LPTTIISAPLLSEDTVHINDRSFSVRESLLRNTFIFNSIGFPALTVPFGYSFHSNMPI
                     GIQIIAKPYNDYKLLMFGNYIETHYMNIV"
     gene            complement(10685..>11671)
                     /locus_tag="VF222_00405"
     CDS             complement(10685..>11671)
                     /locus_tag="VF222_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140918.1"
                     /translation="QEAQTETDQTATGANIGQEAQTETDQTATGANIGQEAQQQANQQ
                     AFGADIDQKITQTATQVAKSSADPDSEDAGDVQQVINQIANQAASGGGKGKVNQIVNQ
                     ISIQIAKDPSGDLAKSLGNLAGFYGSGNKDQVNQAIKQIGTQVAVGDNINQVLVQVSN
                     QVTNNYFKNKIKLDIDNDRDNDNVKIIKKVYKRDNSCPTQSDSIQLKGKILPKGVIVL
                     ADFEPCELKDGRATLNIPANPNLKFVVLFIDKKGNDHEGAIIPKQKVQSLSKVNGLYV
                     VNFDNKMTGIDPVTGKKITLDKINGLALYNVAKKSLDFKSGNSLALTAVLQK"
BASE COUNT         4203 a         1593 c         1629 g         4246 t
ORIGIN      
        1 agtttcttct ttgaattctc taattgcagc atccttagga gattcaccat cttcgatata
       61 tcctgaagga atttcaactt gcactgaatc cactaaatgt ctgtaacttt ttatcaccaa
      121 tattttatct gcagaaatga atgggacaac tgtagaatag ttctttcttt ttcctctaac
      181 gtatatcttt ttaaagccat tgatatccag taaatcttca tatatctgga tatattcgtt
      241 taaatataca tttcgttcat cttctagaac tttccatcca ttgggtgcat tttttagcat
      301 ttctgtagat ataatttctt tatatcttaa ttaatttatc ctgctgaggt gtatgttaaa
      361 tagaaaatat taattatcgt tttgagatgg atttatggtt tcataataaa agtataaatg
      421 acatcctcaa ccacactatc atagttagta atcggaaata atatattgca aaagaatcac
      481 tttaattggc aatcaaatta aattgatgct agtttccaat aagtaatcca aaattccacc
      541 tttttctata attatttaaa cattttttcg agttgaaccg atttaaatta tgcagtctca
      601 agtttggaaa taaggaatgt ttgaaatgac gataacagct atcttaaaaa ttaaaataga
      661 aataagtaaa aagaagatct ttaatccaaa atattaaatc gaaaattttg tatgattttg
      721 gagagtataa attctaaact gatgtgatta atttttattt aacactccta atttttaaac
      781 cttaataata gtgaaatctg catgagatga taactcaaag atatcatcta tttataaatt
      841 aagacatgag attacaattt taattccaaa atctatttcg tatatccaca acttcttttc
      901 tattcttaaa aataaatttt ttattgacaa tatagtttag aatagaagca atagatattg
      961 caattatgat aacccagcca taatctagat tagtcttccc aaacccatat actattgata
     1021 attgtattat tactcccaca gaattaaaaa taaaatattt taaatattgt cttaacagta
     1081 tattatattt catatttcta tcttgaaaag tccaaatttt attaaacata aaattagaag
     1141 ataaggaaac tataattcca aagatactag attgaatatg atcaaggtga aaataagtaa
     1201 aaaagaaata agatacgaaa taatttatga ctagaccaat tcctcctact atatagaatt
     1261 ttgaaagttc gcccttatta taataaattt tttgaatagc aattagaaaa gaattgacag
     1321 gagaagagtt ttcttttaaa tttttatagt ttttcaatta aaatattcct tttctgactt
     1381 tgcatcattt atactttatt ctaaaatacc caaaaaatca attcgcatat gcagattttt
     1441 tatcaaatac tagattttta gttcgtatct ctaaatactt ctacattctc taagcttcac
     1501 actgtcttta ctcctaatga tttcttttag ttcattatag aatagttgct tagatttgtt
     1561 agatactgct ttccactcct cccaaagaaa aatattgtgt aggttggatt catattcttc
     1621 ataaaatagg tttagtatat ggagaaaaat cagaaatttc cgactgattc attattctgc
     1681 gtaactcact tttagaaata atagctttga caatctgttt gtttaacgag aattggctat
     1741 atttgttata ttttcttcta ctatcttcaa ttggtatttt tgcaggaatt catccatctt
     1801 gtctttgtat ccagtaagaa taagtgggac ttttccatta tttgattcaa tataattaat
     1861 ttgcattttt aaactttgga attggtacag agtgctaaat tttgatatct ttcattcatt
     1921 atcaattgaa tcataacatt tccatcaata ctcacacact cccgcttatt tggataaaat
     1981 gtaaatgcga aaaagaaact aactacccct ccaattataa gaacagaatg gctgaaatag
     2041 ctagtctatc ctgttctaaa aatatataca attcataaat atacaaaatg tatatatatt
     2101 ttaagaaaat agaaaagata tcttaaagtt ttgaaataac agtgaatttt aatagtagtt
     2161 tatagtattt ataattaaat gttgaagttt ttattgcaga aaaaaaacat atgcactagc
     2221 tgtaacgaaa aatttaggac ctcagaggga ttgttaaagc atgcaaaaga aatccataaa
     2281 cagattattc gaaaatgcaa taactgtgga aaacagtttt tgaatgaaaa agaaagattg
     2341 catcacatga aggaagaaaa acaaagagaa atagattata gacggcacaa aaaccttaag
     2401 taatacaaat ttaatataat tttttaggaa ttcactaaat tccaaagaac agaataaaat
     2461 aacaataaat gaatttttta taagaaggaa gatatcttaa gaatattcat cttgatgcct
     2521 attcctagtt ataaacaata agttttgaat atatgtacaa aattaactta gcaaagataa
     2581 tataattaat agattaaaaa aattaattga gaaaaatttt aattaccgct tcagaaaaaa
     2641 tcattaatca aattgaaaaa aaatttagac acaactttga aatagacttt gaatactctg
     2701 attcatcaga aggtaaaatt gaggtaatta aaaataatag atgggtcaca atttgtagat
     2761 tttcttcaga tgaaagttta gataatataa ttacaatgtt taatgtaaat tatgagatta
     2821 aatgtaggta aaatttttta tttttctata tctagtcctt aggggtaata cttgaaatta
     2881 aatatattca atcatctcct tttctgaaat ttgaaccatt tcgatttgtc ttgcaaatca
     2941 tcttttataa gacctaatcc gcatccaatt ataaatgagg ataatacagg agaaatcttt
     3001 cccatggata gaatgcaaag caatagtacc cctaattctc catggtggat tcttctatca
     3061 aagaaataga cttttgaggg atgttttctc ttccaatagt gtgttgttat ttcagaaact
     3121 gcaaatccaa ttattgccga tataaggagc ggtaccatat ctaaagaata taatgtaaaa
     3181 taaaaaaagt ataaaattgt tttgaatagg ataacaataa atgaatttaa gtgacagtac
     3241 tatataaaat aaaaatctaa tgtgagaatt aaatacaaca atagtttctg tgtttgtgat
     3301 aaatgaaaaa ttgtttaata gaactttcag atattattac aaaatataaa ataaatagat
     3361 acaatatgat ttcctttaca agaaaatcat tactagattt cgttcaaaaa aaggcaaaaa
     3421 agctcgtcta aagatacata gaatgataat cgacttttac acgagtctag tataattcaa
     3481 tttcgcatgt ctaatattca atagcactcc atacctcaat tacaacgagg aattcattag
     3541 tctgctataa gtcctccagt aaccaattgg agttcaattg ttttaatttg tgtttttaat
     3601 aatactgttt attttcttaa atcttctttg tgaattgttt caaaaagacc gtaggttaaa
     3661 aaatttcctt aacttagcaa attaaatgtt aggaataaaa atgtttaaat tgtaattgac
     3721 tttatttaga tatatttgta ttatttaagt cacataatga attcttgaga attaaaatat
     3781 ccttaagaat tttgctggaa ctattaggtt gaaaaactcc tattcgttca tacaacttta
     3841 tattagatac aaatttatta acaaaattat tacaaataat tttgtcatta tctgaatcat
     3901 catcttttag gataagtata gcattattta cctgagcaat aagagatctc ttaatagtat
     3961 ccccggaaat atgaactacc tcaatagtaa taatataatt tactacgttt tctaacaagt
     4021 taatcgaatt ggagttaact ttatcattag cattggaaga ataagtggtc tgaaaaatat
     4081 caggagagat actataacta taatttcctt gagaagacaa ttttattgta tctttcttga
     4141 aatcgacact tgaattagaa tcatcatcta tatctgaagt ggttaatgag aaaaaaatca
     4201 taaaacatat cattgttagt agtatatcac gctgtttctt caagtatcat taatgatgac
     4261 atacctagat aaaaatctaa taatattatt atagaatatt tatctatata ttgtaggatt
     4321 accattatat acaattaact aattatgaaa agaatttcgt tacgaatatc atgataaata
     4381 tagtgcttga aagtaaagcg aataataaaa aaggaagaaa gtttgttttt ttggaatttc
     4441 tctttttgct gaattgtatt ttgatggttc gttttttact tcgaaataag ataactagat
     4501 aaacaggaat tgaaaataaa ccacctaaaa ttaacatata gacccataaa gaattaaaaa
     4561 gtctatttgg atcaaagttt atatttataa aggttaaggc taatccggat gttatagata
     4621 cggtagaaat tggaagtata aatttatgaa ttcttggaag aatgagagaa taggatccat
     4681 ttctattaac tggtcctaat ataacaacta caaaaaacgt taacccccac cagagaattg
     4741 aagaaaaaaa atgaagatag gaagttaaat attctaaaat aatggtgtcc ataaattaat
     4801 taatttctaa gttttattta atttttgaca gttaccattt taaatcattt caataaaagg
     4861 ggtttgtttc tctatagaat tttctatcac actgttaaga aaagtcaaat gttcccattt
     4921 taatttatat tgattggatg aattaaattg aatatagaaa agatgccgga aaataattca
     4981 aaagtattat tgtaaaggtt aaggaagaaa gttaacctat aaataattaa aatgatcctc
     5041 ctagtatcat ttaatagcta taaataaaat agaagtatat tataaggtag tatttctact
     5101 tcatttggtt taaatctcac tcatatctcg gaaaaatttt acttaattct ctcaaaataa
     5161 acctctgtgt ctgattaaag caaagaaagt ttaaaaaaaa ttggatcata aatttatttc
     5221 cattaagtta ttttttatag tatgtttgat tatgtataca aagtagagtg gagtaagaaa
     5281 ataacagtac ctaatacact gtcatatttt tattttaaac ttaaataaac aaattatact
     5341 atcatgcctt tttcctcttt tagtttttat ctaatcttat tgtgtttgaa gtatccaaat
     5401 ggtagtacct ctagatttat ttcatgaatg agaatgaaat gaacaaaaaa aatgaaatca
     5461 ataataatga aaaaaataaa tttaattatg ataataccaa tataaatata aacataaata
     5521 aaatagaaaa aataatacat caaaaatgga agtcaaaaat agagaaccct ttataattga
     5581 cagaaagtta agcctcttaa actaccatac aaactaacga tttgataaaa tcttcaattt
     5641 tcaaactatt tatctatcta atcatcccta ggaaaacata gttatatttt ggtaattcaa
     5701 attgtggagc actaattcaa tttgaaagtg atataagggt gcaatcgttt gtcaaatgtg
     5761 tggggaaggt attgattggg aaggagaata tactcaaaga atcaaattat gtccaaaatg
     5821 taataaagaa tagcaaaaaa atgcaaaata ttgcagtttt aattattctg gagagcgact
     5881 aattgaaaaa gaaataccaa ataaataata catagactta cgggggatca agggatagcc
     5941 ttataattgt ggttttatta gattaagaaa taacaatata atgttatgaa gacatttgct
     6001 tgtaaaagat aaacttaatt gttatttatt gtaggtacta atgtaatgga atataaattg
     6061 ttaataggaa ataattttat ttttggtaat cctaaaaaga tatactataa agatttccac
     6121 attttcacta ttgatgagga tattgatggt ccatatatcg ataccgaatt atttgataca
     6181 aaaggcagaa aaattgttga gattaaaaaa aataatttat tattgtgtaa tactgaattt
     6241 gataaaaaag ctttaaaaag agatcatatt ttaatactag ataaaagggg cgaaatcata
     6301 attgaatcaa gagtttttaa taaagacaca attattatta gtggtaggtt tataatacat
     6361 aaggtagtat taacaatcac acaaaattat attttgcttc ctgaaggaaa gagaataatg
     6421 catagtaagt ttagatcaaa taacaattca gttaccataa cagagaatgg aattgtatca
     6481 aaaaagtaat gaaacgatat aactagaaac tcgttataat tcagaatttt ttgatattag
     6541 atattattac tatttctgga tatttgtatt ttgtggggaa attgaaaatt gttaatttcg
     6601 actagattaa tttattagta gtattttgat ataaaattta ttgtataact ttttttcccg
     6661 gattcattat atttgttgga tcgaaaataa atttaatttt tttaaataac tcaaaaattg
     6721 tgtttccgta catttgtgat atatattttg ttctacttat accatcacca tgttctgccg
     6781 tgatactccc agacaattta taaactttag aaaacacctc ctttgcaaga gaaccgatca
     6841 cctctttgta tttatttgat gcagtatcaa tcatgggcct ggtatgaaga tttccatttc
     6901 cagcgtgtcc ataaaatatg taatctaatt tgtatttttt atatgtattt aacagaaata
     6961 gtgtatagtc atataggtag ttaggattga ctatagtatc ttcgattaaa ctaataggct
     7021 ttcggcttcc cactgtcatt ttcattacat tattcagagc attttttctc gctgaccaaa
     7081 ttctttcaga actaatatta tctgatgtta cttctaatat ttcgccataa gcagataatt
     7141 tttctctaca taattttatt tgtatttcta tgttaacaag cttgtctcca gcaaattcta
     7201 caaaaagaag agttcctccc ctattgttat taactttaaa atttgatgga ttagaaattg
     7261 agttttcata agacaatata gaataatcaa gtaattctaa agatgaaggc aagaaagtta
     7321 gaattttagg gactgatttg actgctttta gaatgttttc gaatcctatc accattacat
     7381 atttataaag tggaagatca agaattcgga atctagcttg agttgttatt cctaaagtac
     7441 cttctgaaga agcaaatatt ttatgaggaa aatacctttt attattatca attagagtat
     7501 caatacgata accacaggaa ttttttgtta ctttgggaaa ttttaaagaa atatattgta
     7561 aaatatcagg tgaaaataat ttgaataatt gattgatctt gtttggtcta ttagaatcac
     7621 tttctattgt tgctgaatca tttataccat gaggattttt aaataacgat tctataatcc
     7681 cattcgaata tataatttct ataccttcta gaaaatcaat tgtgtttcca taacccaagg
     7741 cgtgtattcc actggaatta tttgctatca tacctccgat agtacaaaaa ttactactgg
     7801 caggatctgg gggaaggaac ttatttcttt ttattaattc tttatccaat actgctttga
     7861 caataccagg ttgtactatt acataatcat tttcaaattc aattatttta ttcatatact
     7921 ttgtaaagtc aagtgaaata ccggtcgtta aagactgtcc taatagacca gtgccagctc
     7981 ctcggccagt aattgttaaa tgtttagaat aagcatattt acagatattg gagatatctt
     8041 ctttatcctt gacttgaata attgtacttg gaagaatggc aaattcactc gcatcaacag
     8101 aataaatttt tttattccag tcatcggaga gaacatttcc tttagcgatg cttcgaaggt
     8161 cgtcatcaat tttttcttga tgcataaaga gataagagag attagaacta taaagttttt
     8221 ataagaaata aaatatctat tgaaaaaata taataaaagt agacatattt aatagttact
     8281 ttaacgatag tattatatgg acgattgttt taggtcgata agatattgtc tggtatgcaa
     8341 agaatatgta attgttaaga catattccca gcaagtagat tcggtaaaat gtcctgattg
     8401 taattctaca ctacctccaa taatggaatt ttgtttgatc gataaaccgt cttatatctc
     8461 ataatttttt tttctgagtc tggagtcgag atatcaatcc caatccctta gtaacatatt
     8521 gacattgctt ttatttatta gaacggagta cagtaattta ggatagtaaa attttctata
     8581 aaatattcgc taatttatag aagaattatt attatataaa aaatcaatcc tgaatttaat
     8641 caaatagttt aagttttaaa ctaataagat ttatttaaat gaggattggc tataacaaag
     8701 ataaaagaaa agtatttgac aaatatttgt atgggtgtcc ttgttgtcat ttatcacgtt
     8761 atgtaatttt atcagaagat atacaagacc cagcatacca taaatatgaa ctttattgcc
     8821 atagttgtaa tgtagtattt tcatctatgc atgcagtcct tccgttggaa tttaaccaag
     8881 aaatttaatt tttcgattat attttaaagt tctcatgtga agacgtaatc atcacattgt
     8941 taattttttt attttttctt tgttcgctta tttaatttaa acataatcaa tgcagctccc
     9001 ccccataaca tgaaggcggt taatttttgt tgaaaaaacc atgctattcc acccaatact
     9061 gccattatta tacctataat ccccaggaat tgtctagtac ttaccaatat cagcaattac
     9121 atttttatat atatattatt tgagttataa aatatatttt cttaattatt cattaatgat
     9181 ttaatttaat attttttata ctatattcat atagtgtgtc tcaatataat ttccaaacat
     9241 taacaactta taatcattgt acggttttgc tattatttgt attcctattg gcatattaga
     9301 atgaaaagaa taaccaaaag gaacggtgag tgcaggaaaa ccaatgctat taaagataaa
     9361 agtatttctc aatagtgatt ctcgtacaga aaaagatctg tcattaatgt gaaccgtgtc
     9421 ttcagaaagc aaaggagcag atataatggt tgttggtaaa accataaaat caaatttact
     9481 atataaagac aaaaattgtt tttttatttt ttttatgtta tttagagatt catagtaatc
     9541 tttatgtttg attttggctc cttctaataa ttgatcccgt agttcaggcc cataatagct
     9601 gaattttttc ttcaaccctc ttttatgaat tgtatacgct tcaaagagtc ttacaatcca
     9661 ccatgatctg aaaaagtcct ctgaattaat ttccacatgg ataaaagaga gaaggttatc
     9721 agattttagt aaatcgataa aattagtaaa tgaatatttt acattatttt ctaagtaatc
     9781 caagaaataa tgacaaggta ccccaatact aatagtccgt ttattttgtg acaatatctt
     9841 ctttaggttc aataaagata atttttgtat ttcaagttca ttaatataac caacaaattt
     9901 tcgccttttt tttcgaatag tactaaatac tattgataga tcgatcgagt tcttacccat
     9961 tattccgata tgatcaagtg aaggtgacaa tttgtatact cctttgttgt caatggaatc
    10021 aaaagtcggc ttaaatccaa aaataccaca aagagacgat gggactctta tagagcctcc
    10081 tgtatctgtt cctaatgcaa agggaattaa ccctgctgca actgctactg cagacccacc
    10141 actagaacct ccagagattc tatctaatga atatggattc tttgaatttc caaataaaaa
    10201 attcttacct gtaatccctg ctgcgaattc atttaaatta tttgtaccta ataaaatagc
    10261 acctgctttt ttcaaacgtt ttattactgt ggaatctgat ttagagatat gctctaagaa
    10321 tatttttgat cccgctgtta atcgtacatt gctaacagca ataatatctt taacagaaaa
    10381 tggtatacca tgaaggattc ctctataaaa tccgcttttt aattcatttt caagtctttc
    10441 tgcttcttgt aatgattgct catttaaaac agttataaaa gaatttaatt ttgggtttaa
    10501 tcttgcaata gctactagat aataatcaac taattcagtc ggctttatat ttccagtttt
    10561 aatttcttgt gaaagtgcga taatagactt acatttggat aaatagcttt tgtccatatt
    10621 ttgtaaatct ggcaatatta aaaatattat cagaaattat tatcaaataa aaaattgaaa
    10681 aaaattattt ttgaagaaca gctgtgagag ctaaactatt ccctgattta aagtcgagag
    10741 actttttagc aacgttatat aatgctagcc cattaatttt atccaaggtt attttctttc
    10801 ctgttactgg atcgattcct gtcattttat tatcaaaatt gactacataa aggccattaa
    10861 ccttgctaag gctttgaact ttttgtttag ggattatagc accttcgtga tcatttcctt
    10921 ttttgtctat gaataatact acgaatttta gattagggtt agctggaata ttaagggtag
    10981 cacgtccgtc ttttaattca catggttcga aatctgctaa aactatgaca ccttttggta
    11041 atatttttcc tttgagttgt attgaatcgg attgagttgg acaggaatta tcgcgtttat
    11101 agaccttttt gattatcttg acattgtcat tgtctctatc gttatctatg tctaatttta
    11161 tcttgttttt gaaatagttg tttgtaactt gatttgaaac ttggacgaga acttggttga
    11221 tattatcacc aacagctact tgagtaccta tttgcttaat agcttggttt acttggtctt
    11281 tatttcctga tccatagaag ccggccagat ttcctaatga ttttgccaag tcacctgaag
    11341 gatctttagc tatttgaata gatatttggt taacaatctg attgactttc cctttcccgc
    11401 ccccagatgc agcttgattt gcaatttgat tgattacttg ctgaacatca ccagcatcct
    11461 cactatctgg gtccgctgaa gatttagcaa cctgtgttgc agtctgagtg attttttggt
    11521 ctatatctgc tccaaatgct tgttgattag cttgttgttg agcttcctgt cctatgttag
    11581 cacctgtagc tgtttggtcg gtctcggttt gagcttcctg tcctatgtta gcacctgtag
    11641 ctgtttggtc ggtctcggtt tgagcttcct g
//
LOCUS       DASVEV010000008        32599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_3341, whole genome shotgun sequence.
ACCESSION   DASVEV010000008 DASVEV010000000
VERSION     DASVEV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 32599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32599
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..173)
                     /locus_tag="VF222_00410"
     tRNA            complement(100..173)
                     /locus_tag="VF222_00410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Thr,seq:ggt)
     gene            875..1837
                     /gene="tfb"
                     /locus_tag="VF222_00415"
     CDS             875..1837
                     /gene="tfb"
                     /locus_tag="VF222_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX7140919.1"
                     /translation="MEKSGEQQKSEKINPKSPSYCDQCQKDSIIFDNSANEFVCSSCG
                     CVINNELLNNENMQGYIVQSDYNDRARTGMPESLAVHHKGLSTLIGMGDIDARGKALQ
                     PKQKMEIQRLRTWNSRSQLNDSVSRNLEKALKYLNNFGDKLSVNHAVIESAAYIYRKA
                     AIKKLAKGRSTIGLVGASLYAACREASTPKTILDISEICNISSKELMAHYKLILNELE
                     LNIPATKGPDYVTIICNRLKLSEKTKREALHIFSKVEQKGISIGKNPRAIAGAVIYLA
                     SQVCDEFIRQVEICQVADISTVSLRKRCKEIKALTEMDNLVNIS"
     gene            complement(1905..2081)
                     /locus_tag="VF222_00420"
     CDS             complement(1905..2081)
                     /locus_tag="VF222_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX7140920.1"
                     /translation="MPLITVSLYPGRSDEQKKLFVEKITESAVEILKTKPEHVIVVFD
                     ENPKENWYWAGKKL"
     gene            2772..3581
                     /locus_tag="VF222_00425"
     CDS             2772..3581
                     /locus_tag="VF222_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140921.1"
                     /translation="MLSVQEWKRWFLYQLIGGGVHNAIYHEHILRELGKHYDINLDRA
                     LRDLIDDGIIMLIESKKNKKYIVNFESLEDAQMIMSLDKNTKVQKTSKIHDHNNVNYM
                     DSTNEKRFVQPYLPEPSGYVYWFDNEENRKFKKQSVYRIYFKETDKTNFAAQLITKPI
                     GSSKIIHMGSLNDPNSYISRLWRAVVSVSEENKEGTFILQQIQDKDRMACGNNRQRGK
                     IALSIFKKLGYIQQSEFKGNSIRFKVSGKKPYSITLDEIFNLKTKNNDRYF"
     gene            complement(3773..4252)
                     /locus_tag="VF222_00430"
     CDS             complement(3773..4252)
                     /locus_tag="VF222_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140922.1"
                     /translation="MSSNHETDSSVENIKSWEVESSSSVEEFDDYSKALDLFNIKSDQ
                     DEDVILYEIQKSKINGSIIKRIPILNTKQSKKRKEELEKERSPKKRTLPPQNQAPIQN
                     KNKPQDIKYRIVILVIIIVAFLILLYVLSQIVVSSDTLNAHFSLGQSKRNSDFPLTG"
     gene            complement(4344..5231)
                     /locus_tag="VF222_00435"
     CDS             complement(4344..5231)
                     /locus_tag="VF222_00435"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7140923.1"
                     /translation="MIREVTIVNTVKNYYELTKPKIWYLLVFTAFGSALTASLLFDIP
                     ITPLTWILLLGGVAAGSAAADTITGYNDRDIDAIMDRTKDRPIPTGRISPRNALIFGL
                     ILTAIALIFSWVINLWVFLLMVFGLFDNIIIYSKWLKRRSQSNIILGGFSGGVPALIG
                     YVTVTTQNLEIGLVMAGLVFVWIPTHIWSLALHVRKDYQKAKVPMLPVVCEEKRAVRI
                     IAGTTLIMVLFSILPFFFNQFGQIYLVTATFFGVIMMGLSLWLLLKPTEKASWTVFKF
                     SSPYLTAVFIAFMVDAFFH"
     gene            complement(5321..6397)
                     /gene="asd"
                     /locus_tag="VF222_00440"
     CDS             complement(5321..6397)
                     /gene="asd"
                     /locus_tag="VF222_00440"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7140924.1"
                     /translation="MLKVALIGSTGAVGQEFVIALKNHPWFELTAIASSKRSAGKRYI
                     DALRDDSTGILKWHNKEQVPDYITDKIVMSVDEIDPKGYDLIFTAVESSDAQIIEPKF
                     AETTPVISTAAAFRYQDDVPILIPGINDDHIELLNIQKKNRNWKGYICPLPNCTTTGL
                     AITLKPILDNFGINSIFMTSMQALSGAGRSPGVAALDIIDNVIPFIPKEEEKVQIETK
                     KILGTLTNDSISSSMIKVSCTCTRVPVMDGHTETVFVETQKPLEPEQVKESMIKFSKS
                     KKMNDLPSSPSEYIIVHDDPTRPQPRIDREINDGMTTFVGRLRKDTVFDNGIKYVLLS
                     HNEKMGSAKGAVLLAELLKTKDMI"
     gene            6457..7668
                     /locus_tag="VF222_00445"
     CDS             6457..7668
                     /locus_tag="VF222_00445"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7140925.1"
                     /translation="MIIDINIIDSEVLLNLGDIYDVNQLNQIQEEKKFNGLNSCSHGG
                     IYSIPNYLKVKIDFSSNINPLGISKTVLKEIRKNINHISHVYPDSNCNILKKSIADYL
                     EHGIDKEWISVGNGATELIHNFVRAVSLKNSIIISPTFCEYELASKRCRMKIDYLPLS
                     KKLEIQADLVIEQSKNNSNSLIFICNPNNPTGRVNTDMIEKIISCIDSAKTILFIDES
                     FIEFLNDIEKKSLISKIKEFDNLAILRSMTKSYGLAGLRLGYLIAHPHLIQKLKRFQI
                     PWSVNGIAQIAGVAALKDQEHICKSKKIIHRERNYMYKKINTKVSHANALRSDVNFFL
                     IKLKNINSIAYQKILLNLHGIFVRDCSSFTGMSNGFIRVAVRTHKDNIMLLKAIYDIN
                     NNLHKYKLMEN"
     gene            7703..8575
                     /locus_tag="VF222_00450"
     CDS             7703..8575
                     /locus_tag="VF222_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX7140926.1"
                     /translation="MNAKVLMVQGTSSGAGKSTIVIALCRIFSNLGYKVSPFKAQNMS
                     SKIHNISNVHKISKIQAIQAFAARKQPEWQINPILLVPLGNNISNIFVAGKFLEEMSA
                     IKYYNEFVLVKGFPIVLDALNKLRNENDIVIIEGAGSPAEINIAKYDIANMLLAEKVN
                     CPVILVSDIERGGCFASIFGTIKLLKIKHQRLIKGFIINKFRGDENILEYAIQFIERR
                     TKLNNLGIIPKFEFFLPPEDSLDGTNNNNNLTNLNKFSSKRKLNEQIDLLAATIGNSI
                     NIEFILKQVLKIEQ"
     gene            8572..9546
                     /locus_tag="VF222_00455"
     CDS             8572..9546
                     /locus_tag="VF222_00455"
                     /inference="COORDINATES: protein motif:HMM:NF002281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX7140927.1"
                     /translation="MISTFHLITILLLSILLDLKLGDPNNRIHPVAWLGKYINYFIPK
                     IKNGRNKKYEKINGVIFTSITITFFAIILHISIVYLYHITFILMMILSTFILFSVIAI
                     KGMEKHITAITTALQNNDIENARINLSMIVSRNTESLDEQHILSGTIESIADSTVDGI
                     LSPIFYFSLLGPTGAFIFRIINTLDSMMGYKESYFENIGWIAAKADTYANYIPSRITA
                     ILMIFAAIITNADWKNSIITFRKERNCTVSVNSGQPISVLAGALRVRLEKLEHYSIGK
                     PLEKISIEKCKMAIKIMKITILIFCVIIASPLIIILGYLNWWNFIFGI"
     gene            9536..10273
                     /gene="cobS"
                     /locus_tag="VF222_00460"
     CDS             9536..10273
                     /gene="cobS"
                     /locus_tag="VF222_00460"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX7140928.1"
                     /translation="MVFNNISSVLSFLTIIPTKSYDITFIAQNMYLFPLIGLIMGLVI
                     GVLSFFLFSFLQGFLLGFIITATIIGITGLHHSDALSDFADGLMAKGSFEFKHKVMSD
                     PRNGTAGTVILIFYIIGMIILVSYIDEKPKLISAIIIAEVLSKYSMVLQCYLGNSAWK
                     GMNSIFIKEMKNKKKFLVSNLILLASIITFGFNFYYMVLSVITMLSVSIMLLLIANRS
                     FGGISGDTIGATNEISRFFIYLIHTIF"
     gene            10227..10940
                     /locus_tag="VF222_00465"
     CDS             10227..10940
                     /locus_tag="VF222_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX7140929.1"
                     /translation="MRFQDFSFILFTQFFNKLTDKVSQKFNKKIIAVIMAGGEGSRMK
                     RDYKTEKPLITIKGKRLIEYVIEALIKSTCFHKIVACVSQNTYKTMKFLQDYNYNCEQ
                     PIEIIEGKGNGYSSDVSFIIENFFEHIIFMISADLPLLSDIDIIKILSKCNFDATCYS
                     ILFDKKIVDEICIKPSVVFEYKKRNYCYSGINIFNLTKRHNDQTLIGERYIIINRIGI
                     AVNVNEKKDLLVARKLIKS"
     gene            complement(10984..11178)
                     /locus_tag="VF222_00470"
     CDS             complement(10984..11178)
                     /locus_tag="VF222_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HEX7140930.1"
                     /translation="MKENILIPKPNSAFLNIQCSKCDEKNIIYSHTTSNIYCKSCNEL
                     LAEKSGGKAKIYGNLLSSLD"
     gene            complement(11198..11476)
                     /locus_tag="VF222_00475"
     CDS             complement(11198..11476)
                     /locus_tag="VF222_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HEX7140931.1"
                     /translation="MKMQKEMRKFCAKCKSHTTQAVSIYKKGKDRKTALGARRHAEDK
                     KGYGGQKFPELKRTAKTTKKITLRYTCKTCQRKSMREGMRIRKLEIGA"
     gene            complement(11915..12526)
                     /locus_tag="VF222_00480"
     CDS             complement(11915..12526)
                     /locus_tag="VF222_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140932.1"
                     /translation="MTHEVNYHDFRSKTGIESVSVFKQLLKFLGKNRIGRLLDKNGEQ
                     VFLFSEMDRIRASLLALQLGCDIQECSKILSWKDFEYFTCELLSLFEYNTRVNIVLSK
                     PRAQLDVIGIKNDFAITIDCKHWNYNNKTPLTIYAEKQIGRTMLWLKREKKITKALPM
                     IITLNDSIFKFIKGVPVVPILSLKSFLKEFDKSDESMLLINKN"
     gene            complement(12633..12920)
                     /locus_tag="VF222_00485"
     CDS             complement(12633..12920)
                     /locus_tag="VF222_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7140933.1"
                     /translation="MSIPNQNSNDEETRRREAEAEAMRQRVLLVLLEPSARQRLANVR
                     IVKPELAGAVENYLINAASTGRLNRALTDDELKQILLNIQAPKKDFKINRI"
     gene            complement(12934..13386)
                     /locus_tag="VF222_00490"
     CDS             complement(12934..13386)
                     /locus_tag="VF222_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HEX7140934.1"
                     /translation="MAKVYDVPSDRFIEKLATYLKNDKKIEPPAWSYFTKTGPHTEKI
                     PQNKEWWYIRCASILRKVYIHGPVSVEDLRSKYGGSKQRGYNLAHHVDASGGIIRTGL
                     KQLESSGYVVKKKNGRIISDDGMKRVDRLATEIHKELQKTNPELSRYS"
     gene            complement(13453..13752)
                     /locus_tag="VF222_00495"
     CDS             complement(13453..13752)
                     /locus_tag="VF222_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit"
                     /protein_id="HEX7140935.1"
                     /translation="MGKHQAKLLAQERIVILLHNSVQEIGQDYKLANEQAQLAKKIAK
                     RLRLKLPYDIRQLYCKNCKLFICPGFNCTVRIGRSKIKALRITCLSCNTVYRKLI"
     gene            13874..14602
                     /locus_tag="VF222_00500"
     CDS             13874..14602
                     /locus_tag="VF222_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis protein"
                     /protein_id="HEX7140936.1"
                     /translation="MTSHISVVLAESALEIIPPEVRNTQLIKNYSKKVGKEICELFLD
                     KSYHYSAMKEKNIDFIWKRGRPDLVHICLLSILSTPLFLKNLVDVYIHTIDNKVIFIG
                     QQVRIPKSYRRFEGLMIKLYKEKEIKSNDKDYNKCLLKIEKNLTFDNLVDKVIKPDKI
                     IGFSSTGILKDLKNIVNENIETNNKKISFVIGGFQGGHFSQSTRERFHMTYSIANEQL
                     EAHVVISRLVYECENRLSFLDGPS"
     gene            14667..16022
                     /locus_tag="VF222_00505"
     CDS             14667..16022
                     /locus_tag="VF222_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140937.1"
                     /translation="MSFIFSIQLIAFIILFNILLLYPIDYAFSDGLFEERLPPASVGD
                     REASLYTKINPPVLTSDTKENAFFELRLFDAKTDENIKFVSYFIAVEKNDKLLMRDLF
                     HSPTGPLKLKINPTSSESISVFGSTEPFLGGWTSETGDITINGPLLLQGGLYHFAIEI
                     FGIDNPRNIFTPENAPRFDSWLSVGDVYRDNIIDSEKKYNITLISYYDQIQNFKYDSQ
                     QSNMSWIMPFNWDLKRIQDNNIFVHEEIKIPKTFTKFSETNAFEAVVNGNPLVGRSIA
                     LDPFTEENNFIIHLLLNKAEIIKIAETINNDAKNNNTNNTSSTMTFSLSPAQQKIVET
                     TSEVVSDTGGILVNFIWDEQILSNQDTNLQLRFSDALSTEEINGDIKYDIIVYDKSGN
                     KIITKQDLIAQNATDSQVIKFPSKGIYQMELNIKGITYPDLGTPDETRKGIARGTVVV
                     S"
     gene            16119..17108
                     /locus_tag="VF222_00510"
     CDS             16119..17108
                     /locus_tag="VF222_00510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7140938.1"
                     /translation="MKIQFVNHLVCLNHSDKQIRLKLNNLISNNQNECIEGFLYCIKC
                     DTKYPIIEGIAIVLKDMLDYIQNRTEMYGKWLLHCQSKEMKEYLKEIGKTITVSNKPN
                     TYEDGGIWYLPYKWFQQENHKEDRLMKSLKGSPNPNELYDRVINSINPKIDGIALDIG
                     CAMGKTTLQLAQKYSFVIGIDLSFSFIKEARKYMQEFKQGNVEFCVADAESPPFHPLK
                     FDLTIAINLIELVNTKKLLSSCHWLLKPQSDIIITDPYDFNRERILDKTYDAQSFRTL
                     IEENGFEINESCLKKEAFIPWILKINERVYLFYFVDFIKAKKISKHKVILNKY"
     gene            17177..18346
                     /locus_tag="VF222_00515"
     CDS             17177..18346
                     /locus_tag="VF222_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7140939.1"
                     /translation="MYESIIFKDRKKLSPRYIPKEIPHREKQIDLLIRTFLDIKEDPD
                     KFPLTILQIIGPAGIGKTSTVIKFSNILENELRKSKINIKIVYINLKLQGGNKYAIYK
                     YLLSCIAPELPAQGLSAEEMLRQMLDYLIINNVYSIVILDEIDYLIKIAKEIGIIYDL
                     TRLNEFDPSKKCNVKGVIFIARSTEFYEKLDEAELSSMGRTYIEFPNYTIEQISDILI
                     RRSRDAFQENVIGTDIIDWIAKIVVSPVVNGDIRYALDLLSYAGNLAESEGTERVLID
                     HVKKINKQIYNGITDDDIKELSNIQIIVLLGIIKGLKIRNRDYVDLKEIRMQSSEISE
                     KYKIRKLDVEDILDDLATRKIIKILSLKKISLTSSSIENLEKILISKIDSHPDSK"
     gene            18343..18639
                     /locus_tag="VF222_00520"
     CDS             18343..18639
                     /locus_tag="VF222_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140940.1"
                     /translation="MNDTYNVKDIVEQGLGSIGKIKILRALAEEKKLLTVYGLHKKTH
                     LKREDIKRNLAELIKIEWVIEQKISNSLYSINRENKYMQRLVLFFYEIGYIENI"
     gene            18893..19432
                     /locus_tag="VF222_00525"
     CDS             18893..19432
                     /locus_tag="VF222_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140941.1"
                     /translation="MKLKNYKTTRIGQIFIFALIGILSYFTITTISFAQAQEENLNVI
                     VTFFDITDDTGDVITFINADDFTKAKIFTPLKSTKNGNTTELQFNLTASQVSMGEKFR
                     VCALLLQQDGIICKVGQNSEADRPEIIDFRLATDDIVKIDVENISVQDLGNNKNDNAN
                     DNDNKDDDEVEESKDNNNN"
     gene            complement(19566..20738)
                     /locus_tag="VF222_00530"
     CDS             complement(19566..20738)
                     /locus_tag="VF222_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7140942.1"
                     /translation="MGKKIIILGAGFAGLCISNILRKNLDFTHDIIVIDKKDYFMMGL
                     ANLWILDGRRRLEDSKISLKNLESKGITFIQDEITYIDTKSKLIQTLHHGDRLHYDFL
                     VIALGAEMSTSMIPGFIKQNSYNLYDGESIPCLRKKILSLEKGKIAIVIMGFPYKCPP
                     APYESALIIDQIICRVDRRNKIDIDIYFPSKLPLPVAGEQPNSKLILILKKQNINLYP
                     NYTLKEVNKYDLEFHNGRKKEYDILVGIPFHFPPSVLKKSGLIEEKGNWMSVNKSTLE
                     TKFNDVFAIGDVTEIKINDIISIPKAGIFAEGQARSVASQIIHKIIRIDSQEFHQIVQ
                     FDGKGFCFMDTGNGKAGFIDTNFYNSNGPITILKEPSTLYYKKKINFEKQRMKEWL"
     gene            21359..21745
                     /locus_tag="VF222_00535"
     CDS             21359..21745
                     /locus_tag="VF222_00535"
                     /inference="COORDINATES: protein motif:HMM:NF042555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX7140943.1"
                     /translation="MVSIAMDYNKLCKSIFALHDDIRYAGVVDETGALLAGGMKKGID
                     SIVDEAGEELYLAQTAIRRSMREKFDPTMGKARFSYIEREKLSVITLYLDNKVVLITM
                     EPNIDSHTAIDIAEDSLDMLDGKIDI"
     gene            complement(21821..24202)
                     /locus_tag="VF222_00540"
     CDS             complement(21821..24202)
                     /locus_tag="VF222_00540"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7140944.1"
                     /translation="MEPIITSTSWNHEIESQVLKSWDSENLYSFTLKKNDGSDSVFVI
                     DTPPPYPSGKPWHIGAAAHYAQIDMIARTARMNGHNVMFPIGIDRNGLPVEIYTEKKY
                     KVRMRQVPREKFLELCKTALDDLEKEMIEIMINLGLSSNFKEYYRTDSIEFRTLTQKT
                     FIDLWNEGLIYISNRPNNYCIDCGTTISDAEIIYDDLPTNLIYMFFSIADNNNDKIVV
                     ASTRPELLFACQAVIVNPKDERYSQLIGKDVIIPLFNRKVPILTHHSAKPDFGSGVVM
                     VCSYGDHNDVQVFRELNLKEIISVDENGYTTPSAGKYSNLKLKHARQKIIDDLRNTSF
                     IEREENIMHRTPICERSKTQIEIIPLQDYYLKQLDYIPILKAFTKKLNFYPPVHKQIL
                     KNWLNAVSIDWPISRRRFYGTEIPIWFCQNCKSPNLPKSGNYYQPWKEKPPFDTCAKC
                     GFNEFIGEEKTFDTWMDSSITPLFISKFSQDSQFYCRTYPTTIRPQAKDIIRTWLYYT
                     MLRCYQLTEKLPWPNVWIMGYGVDEKGKKMSKSKGNVVDPFPIIQKYGADTFRFWSAS
                     ETNLGQDFRCSEQSISNSQKFLSKLWNLGRFLSSFEVPKEFSTEPLKNNNNLLHTDKW
                     ILSELDKLINTCIKGYDNYNFFIPATSIRDFTWNVFASHYVEMIKGRAYDNQNKNFQV
                     SSLYTLHRCFSIILLLVAPICPFITEKLWKTLYSSESIHIQRFPKTNDFFKDFEKYTE
                     KIIEFNSLVWNKKKNSINPNTGKPLSLKDATSINVPSELNEFEQDLKTMHNLI"
     gene            complement(24289..25107)
                     /locus_tag="VF222_00545"
     CDS             complement(24289..25107)
                     /locus_tag="VF222_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX7140945.1"
                     /translation="MSNIRNIVENDIKYLNDKGDYLLNRTQLSLSNLLSFLGKKYEVN
                     SQIKIPGGEVITVDFKTDDEKYIEVIDSNDDLIKFNRIKSIYPELKIVAIGSSKYKGK
                     LTEFESIFFFDTKYEKIGSIFIEDPSLSFDYAHILPLVEKCSILHGHTSTVMVEIIGK
                     MKNGLVIDFSEAKMLIKEAINDLDHKFFVNNRYLSKDDDLYYYVRFDGPQGYFDLQMP
                     KSTVYLLSDEATVENLSKEILKILAPKMPKNIDALGVYIYEGVNKGAHLLADVR"
     gene            25283..26083
                     /locus_tag="VF222_00550"
     CDS             25283..26083
                     /locus_tag="VF222_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7140946.1"
                     /translation="MSLDLTRIIISSIKNCILTDETFGIAFSGGIDSSLLAKISSDIY
                     KENVILLSIGFPFSHDLEFSRRIAQELNMKHFTYEIKNEEFNNISRKVIDNLECNNIS
                     HIENCIAFYFISFLSILNNCKLVLTANGFDELFCGYNRYRQIILQGQEQINSFMKQRI
                     TNELTLVNEINNTSKEFNVKIKQPFLTKRFIDFAMKIPLEKKILGPSDNLRKHILREI
                     AISLNVPIESAMKPKKALQYGTLIHKNLSKIIDTDNEIKTKLLTKIKH"
     gene            complement(26133..27494)
                     /gene="purB"
                     /locus_tag="VF222_00555"
     CDS             complement(26133..27494)
                     /gene="purB"
                     /locus_tag="VF222_00555"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7140947.1"
                     /translation="MVILPIDSGRYGSKDIKEIFEEQKRLDFILEFEAATAQAQSKIG
                     IIPIDAANEINVLAKSGKITLTRTKELESTTDHDMAAVVNAMSEKCSPNTKPWVHFGL
                     TSNDIIDTTTSMQLRASLLIIEPKIKELAKILSNKALEFKIQPAVGRTHGQHASIIPF
                     GLKFAVWAMEMLKHIERIKEGKKRFLICKTLGVVGTGSLMGDKALEVQQMVALTLDLH
                     PIEAATQVIPRERLAEAQFLIALIGTSLDKIAIEIRNLQRTEIDEVAESFRSGQMGSS
                     AVPVKKNPIKSERVSSLAKLLRSLLSVSMENICLWHERDLSNSANERFTIPMGIILLD
                     EMLTTMIKVVKNMFVNIEKISSNINITKGQIYAEFILEALVRKGIPRIEAYMDIQRVA
                     FEGKDKGKQFIQAIKEDSVLSKYFSEMELNDIFTPEQHFASGIKIIEKVSSTVRNELS
                     TSK"
     gene            complement(27565..28500)
                     /locus_tag="VF222_00560"
     CDS             complement(27565..28500)
                     /locus_tag="VF222_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7140948.1"
                     /translation="MMFRTLLFIPGNNKRFLEKSKLLYPDILCFDLEDSVPVSEKSIA
                     RDMIIQSFSSTNSLHPVNININNNNNKINDSNYNFKDSPQLFVRINSFESGLYEQDLE
                     SVICEKLDGIVIPKVNSDSELKKITEIIENLEKKRNVKKIIRLIPSIESSQGVVNSYL
                     IAKFNSRVCSLLFGVFDYLYDMKLDYENEGLEYSYARAKIPVDARAAGIPALDSIWQK
                     VDDLDGLQGDAKTAKRLGYTGKSIIHPKQIEPVHNVFVPSQNEIEWARKVISTLNEIQ
                     EHGDKRGAFKVDGRMIDEVHFKQAKLILDFITKDE"
     gene            complement(28540..29589)
                     /locus_tag="VF222_00565"
     CDS             complement(28540..29589)
                     /locus_tag="VF222_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7140949.1"
                     /translation="MKAAVFREYNKDPTQVVKIEDIDTPKLKPNEVMIKVESASYNYN
                     DLWAIWGEPVKTPLPHISGSDVAGPVVEVGEDVTKFRTGDRVVSHSNLSCRICEMCTA
                     GREYDCNERNIWGFQTGPLWGGFAQYTHLPEVNVAKIPDSVSFDDAAAISMVGMTAWH
                     MLVGRAKIKPGQTVLIMGGTSGVGMAGIQIAKLYNCDVIATAGNQEKMDTCMKLGADQ
                     VVNHREPDWNKKVRNLTNKQGVDVVFEHIGKAVFPQEVSLLKMGGTLVATGATTGYDT
                     TLDLRFLFFKGTNLLGSTQGTKSDLEDIIYWTGRGKIKPLIATKLPFSDMVKGHVMMA
                     NADQIGKILTNPQEL"
     gene            complement(29759..30391)
                     /gene="cofC"
                     /locus_tag="VF222_00570"
     CDS             complement(29759..30391)
                     /gene="cofC"
                     /locus_tag="VF222_00570"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX7140950.1"
                     /translation="MKIAAIIPMKSLHLAKSRLSNILTPEQRKYLAMYLLDATIKELK
                     ESKFISEIIIVSSDKAVKKYSCLKNLKFIKDYDEGVNNAVILADHYCINNDINANIVI
                     PQDLPFISAKEIDEICNISNKYHKCIIICPSKRFDGTNVLFRKPPNVIKTFYDDNSYI
                     NHLKEAHKFKIPIESIDIGKLRFDIDTKEDLLELIPLENWNFVLKDFEIN"
     gene            complement(30388..31314)
                     /gene="cofD"
                     /locus_tag="VF222_00575"
     CDS             complement(30388..31314)
                     /gene="cofD"
                     /locus_tag="VF222_00575"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX7140951.1"
                     /translation="MITVLAGGTGSIKIIRGLASCTSNLSIISNIGDNFWFNGLYVCP
                     DIDTCIYGIAGILDKERGWGIRNDTFSYLNCMKLLGEETWFQIGDKDLITHFIRTKML
                     SKGYSLSTVVKWIALNQYNIIKKIIPVSDTHVETRIITNKGDMNIQEFWVKYKAKPRV
                     LDVYYKNSEKAKCNKDALRIIRSSDIIIIAPANPISSIGPILSLNQIKEELIEQRDKI
                     IAISPFIGNKAISGPARKYLSAKGIESSVLGIAKFYSQLISKIVIDNSDSNFSKEINK
                     IGVSSFETNILMNNSKEEKRLASFILKVMNYQ"
     gene            31456..32502
                     /locus_tag="VF222_00580"
     CDS             31456..32502
                     /locus_tag="VF222_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140952.1"
                     /translation="MNRIVVLSCWIIGLILIPTAFSQSFASQMEVFLLPESDAADPVF
                     TTIRFVTIQYEPGSELAKIFNGTNERITFTLNSTTQGMDELQSSINEAILKDKKSPIQ
                     LSNSKLDYTAQIRGDEERAQISYKIKFVPHITNYVLPSNSTTPALDLDWRSVVVQGPL
                     TVTVPDYGKFDINYPIGPVKALFPAAAQKLLSVEQTKNIFETPLLDYEEIGFPMDRWH
                     FLFDPTGSQASAAGSGYAEEGGSKVVSVFSLGESSFREGTHSAKESSISTTIDGSKVN
                     IKSSDPPPSAQIQIAGFAQIKKISNAEIAFVSPKGPSGTATATGGFPLQVLLILGGMM
                     DAVAILVLIKARKP"
BASE COUNT        11517 a         4801 c         4850 g        11431 t
ORIGIN      
        1 ttggtaaggt tttattttca caattcctca aattctcgaa taacgtaggt ttattaatct
       61 ctttaaactg aaattcatca ttatttgacg aaaatgaaaa gccttgagcg ggatttgatc
      121 ccgcgacctt ttccttacca aggaaacgct ctaccaggct gagctaccaa ggcacaaggg
      181 aaattttact atttagatta tcgtagattg aatctagagt ggataatata aatgaaagat
      241 ttagaattga gatcttaaat aaatataaga gattatatac tattattcta gttttcaaga
      301 aatattatag tatgtatgtt aaaaaaatat ttccagtagt gtagatatta tacatctcaa
      361 tgagtcatca caaaatcaaa agccttaaca tacctgaaaa taaaatagtc agaatattat
      421 taattatata agaaaagaat ttgaatctcg taaggtttga ataatgattt tattatgaaa
      481 ggtaagccaa agaaaaataa atcaaattat tatagtaaat catgaagtac taagaataca
      541 cgtattgtag atttgtatgt tatttcagac acttatagaa aaaatctata tataaactat
      601 ctaaacaatt caaaaaattg agagaaataa ttatattgaa aattgtacta gaagaaattg
      661 gtataagaaa tatcatactt aattatatag aaaatggaat atatacatta ttctttaata
      721 aagttagatt tctttacaat ataacatatc gaaattagag aatcataatt attgaaagaa
      781 aataataaaa tatcaatgtt tttgaatagt ttcgtagaaa tttctaattt ctagaaaaca
      841 tataaataaa ataattgttt actatcaaat ttcaatggaa aagagcggag aacaacaaaa
      901 atctgaaaaa ataaacccga agagcccttc ttattgcgat caatgtcaga aagattccat
      961 catttttgat aattctgcaa acgaatttgt ttgttcttct tgtggatgtg taattaataa
     1021 tgaattacta aataatgaaa atatgcaagg ttatattgtt caatcagatt ataacgacag
     1081 ggctagaacc ggaatgcctg aatctctagc cgttcatcat aaagggctat ctacattaat
     1141 aggaatgggg gatatcgatg cacgaggtaa agctttgcaa ccaaaacaaa aaatggaaat
     1201 tcaaagacta aggacatgga atagtcgatc tcaactaaat gattctgttt caagaaattt
     1261 agaaaaagct ttaaaatatt taaataattt tggagacaag ctctctgtaa atcatgcggt
     1321 tattgaaagt gctgcatata tttatagaaa agctgctatc aaaaaattag ctaaaggtag
     1381 gtccacgata ggattagtag gggcatcttt gtatgctgcc tgtcgtgaag catcaactcc
     1441 taagactatt ttagatattt ctgaaatttg taatatatca agtaaagagt taatggctca
     1501 ctataaactc atattaaatg aattagaact taatattcct gctaccaaag gtcctgatta
     1561 tgttaccatt atttgtaata ggctaaaatt aagtgaaaag actaaaagag aagcattaca
     1621 catattttca aaggtagagc agaaaggtat ttcaattggt aaaaatccca gagcaatagc
     1681 aggagcagta atatatttag catctcaggt atgtgatgaa ttcattagac aagtagaaat
     1741 ttgtcaagta gccgacattt caactgtttc tttaagaaaa agatgcaaag aaattaaggc
     1801 tttaactgaa atggataatc tcgttaatat ctcataactt gatgtagagg tacgtaggga
     1861 ataggaaaaa aataaacaga agtattgaag gtcattagaa ttatttatag tttttttcct
     1921 gcccaatacc aattctcctt tggattttca tcaaatacaa ctattacatg ttctggtttt
     1981 gttttaagaa tttctactgc agattcagtg attttttcta caaataattt tttttgttcg
     2041 tccgatctac ccggatacag agatacagta atcagaggca tttaatattt tttaatataa
     2101 ctattaaata aactattcaa taaaaaatat taattaataa tattaatttt tatttctata
     2161 ctatataaga gaggattttt agtatatgta tttttatgtt taatataaaa atcaaaagaa
     2221 tttacgtact tcttaatttt aagtagcatc attatttttc caaaaacata ctatcagtaa
     2281 gtaataacaa cattcatgta agcaatattg aagattatgt aaatttgtaa aatatttgca
     2341 atctatttat tagatcgaat tacaaaaaaa ctatcatata gacaaatatt tactactttg
     2401 ataattaggt agacaaatat ggtagtgata tatatccaga tattcctata tctgtttata
     2461 ttttcttgtt ttcagcatgc tatcaaaccg ttgataaaat attgtttaat attgatttgc
     2521 cgaattagaa taataataat tccaaaatat ttataacata ttgaatttaa ttttttgata
     2581 aagcaattgt tgctgtaagt ctttttttaa agattataca tcgttttgtt ataaagtgtg
     2641 ggaagatcat tattcacaac ttatgataca attttaggct attacataca aaaatgttac
     2701 aacaaagaaa ttaacgcttg aaatctgatc tttatgctta tatattcgta gaaaatagct
     2761 tttaatatct aatgcttagc gtacaagaat ggaaaagatg gttcctatac caactaattg
     2821 gtggaggagt acataatgca atataccatg aacacatctt aagagaactt ggaaaacatt
     2881 acgatattaa cttggacagg gcattgagag atttaattga tgatggaata ataatgttaa
     2941 tagagtcaaa gaagaataag aagtatattg taaattttga aagccttgaa gatgcacaaa
     3001 tgataatgag tttagataaa aatacaaaag ttcaaaaaac tagtaaaatt catgaccata
     3061 acaacgtgaa ttatatggat tccacaaacg agaaaagatt tgttcaacct tatcttccag
     3121 aacctagtgg atatgtttac tggtttgata atgaagaaaa taggaaattt aaaaagcaaa
     3181 gtgtctatag aatatacttt aaagaaacag ataaaactaa ttttgctgcg caattaataa
     3241 caaagccaat aggaagttca aaaattatac acatgggttc tttaaatgat cctaattcct
     3301 atatatcgag attatggcga gccgtagtat cagtttcaga agagaacaaa gaaggaacat
     3361 ttattcttca acagatacaa gataaagata gaatggcatg tgggaataac agacaaagag
     3421 gaaaaattgc cttgtcgatt ttcaagaaat tgggctacat ccaacaatct gaattcaaag
     3481 gcaattccat tcgattcaaa gttagtggta aaaagccata ttcaataacc ttagatgaga
     3541 tttttaattt aaaaactaaa aataatgatc ggtattttta ggcgtaaatt gagttaaagc
     3601 attctctatt acgttatcat ctccttctgt ttatattaga taacaagcta tttcattgat
     3661 tccattcttt ttcttactgt agagaaattc attttatttg gtcaactatc gtgatgctag
     3721 aatatttgtc ttcattaccc aatactaatt atagattttg atttagaagg aatcaaccag
     3781 ttaaaggaaa atcactattc ctctttgatt gtccaagtga aaaatgagca tttaatgtgt
     3841 cagatgatac aactatttga cttaaaacat ataataatat taaaaaggca actattataa
     3901 ttaccaagat tacaattcta tattttatat cttgtggttt atttttattt tgtattggtg
     3961 cttggttttg tggtggtaga gtacgttttt ttggggacct ctctttttct aattcttctt
     4021 ttcttttctt ggattgtttg gtatttagaa ttggtattcg cttaatgata gaaccattta
     4081 ttttagactt ttgaatctca tacaaaatta catcttcatc ttgatctgat tttatattaa
     4141 atagatcaag agcttttgaa tagtcatcga attcttcaac tgatgatgac gattccacct
     4201 cccatgattt tatattttcc actgaagaat cagtttcatg atttgaggac attaattata
     4261 gtaatatttg gatattattt aataatttac ctgtttagta ataaccaaag ataatgtaat
     4321 aaaatgaatt cgtcagtatc agattaatga aagaatgcat ctaccataaa cgctatgaaa
     4381 acagctgtaa gataaggact cgaaaattta aaaactgtcc acgatgcttt ttctgtaggt
     4441 tttagtaaaa gccataatga aagacccatc attataacac caaagaaagt tgcagttact
     4501 aagtatattt gtccaaattg attaaaaaag aaaggaagta tactaaataa aaccattatt
     4561 aaagtagttc ccgcaattat tcttactgct cttttctctt cacaaactac tggcaacata
     4621 ggaactttgg ctttttgata atcttttctt acatgtaaag ctaagctcca gatatgtgtt
     4681 ggtatccata cgaatacaag accagccata actaaaccaa tttctaggtt ttgtgtagtt
     4741 acagtaacat atcctattaa tgcgggcacc cctcctgaaa atcccccaag aataatattt
     4801 gactggctgc gcctctttag ccatttactg taaattataa tattatcaaa taaaccaaac
     4861 accattaaca aaaataccca cagattgata acccaggaaa atatcaatgc aatagcagtc
     4921 aaaataaggc caaagattaa tgcattcctt ggagatattc tgcccgttgg aataggccta
     4981 tctttggtcc tatccattat agcatcgata tcacgatcat tgtaccctgt aatagtatca
     5041 gcagctgctg aaccggcagc aactccgcca agaagaagaa tccaggttaa aggagttatt
     5101 ggtatatcaa ataataaaga ggcagtgagg gcagatccaa atgcagtaaa cactagtaaa
     5161 taccatatct ttggttttgt taattcataa taatttttga ccgtgttcac gattgtgact
     5221 tctcggatca ctacctgaat ttcaactctt tatcaaccaa cctaatttat catacgctta
     5281 aatattaatt cttcaaattg gattctaaat taatataaag ttaaatcata tctttagttt
     5341 tgagcaactc agctaaaagt acagcgccct ttgcagagcc cattttttca ttatgcgaaa
     5401 gcaagacata ttttatacca ttgtcaaaga ctgtatcttt tctcaatcta cctacaaatg
     5461 tagtcattcc atcattgatt tccctatcta ttctaggttg agggcgggta ggatcatcat
     5521 gaactattat atactcactt ggagaagaag gtagatcatt catttttttt gatttagaaa
     5581 actttatcat agattcttta acttgttcag gttctaaagg tttttgtgtt tcaacaaaga
     5641 cagtttcagt atgtccatcc attacaggta ctcttgtaca ggtacaactt actttgatca
     5701 ttgatgacga gattgaatca tttgtgaggg tacctaagat ttttttagtt tcaatttgaa
     5761 ccttttcttc ttcctttgga ataaaaggaa taacattatc tataatatca agagctgcaa
     5821 cacccggaga tcgtcctgcg cctgagaggg cttgcatgga tgtcataaaa atagaattga
     5881 ttccaaaatt atctaggatg ggctttaatg taattgctaa tcctgtagtg gtacaattag
     5941 gtagcggaca gatatatcct ttccaatttc tatttttctt ctgtatattc aataattcta
     6001 tgtggtcgtc gtttattcct ggaattagta taggtacatc atcttgatat ctaaatgcag
     6061 ctgcagtact gattactggt gtagtttcgg caaactttgg ctcgatgatt tgagcgtcag
     6121 atgattccac tgctgtaaat attaaatcat acccttttgg atctatttca tctacactca
     6181 ttacgatttt atctgttata taatcaggaa cttgttcctt gttatgccat tttaaaatac
     6241 ctgtactatc atcacgaagt gcatcaatat atcgcttgcc agcagatctt tttgaggaag
     6301 ctattgctgt cagttcaaac catggatggt ttttgagtgc aataacaaac tcttggccta
     6361 ctgcacctgt agagccaata agtgcaactt ttaacataat aatgactatt taagatactt
     6421 tttataatct ttattcaagt ttaaaaaaac atttacatga taattgatat taatattatt
     6481 gatagtgaag ttttgctaaa tctcggtgat atttacgatg taaatcagct aaatcaaata
     6541 caagaagaaa aaaaattcaa tggtcttaat tcctgttccc atggaggtat atattcaatt
     6601 ccaaattatt taaaagtaaa aattgatttc agttcaaata taaacccgtt aggcatttct
     6661 aaaacagttc taaaagaaat tcgaaaaaat ataaatcaca tctctcatgt ttatcctgat
     6721 tcaaactgta atatactaaa aaaaagtatt gcagactatc tagaacatgg tatcgataaa
     6781 gagtggataa gtgtaggaaa tggagcaaca gagctcattc ataattttgt aagggctgtt
     6841 tcgttaaaga attctatcat aatatctccc acgttttgtg agtacgaatt agcatctaaa
     6901 agatgcagaa tgaaaataga ttacttgccc ttgtcaaaaa aattagaaat acaagccgac
     6961 ttagttatag aacaatcaaa aaataattct aatagtttga ttttcatttg caatccgaat
     7021 aatcctacag ggcgagtaaa caccgacatg atagaaaaaa tcatttcttg tattgacagt
     7081 gccaagacaa ttttattcat tgacgagagc tttatagaat ttttaaatga tattgaaaaa
     7141 aaatcgctga tatctaaaat aaaagaattt gacaacctag caatacttag gtcaatgaca
     7201 aagtcgtatg gattagcagg attgcgttta ggttatctga tagctcaccc tcatcttata
     7261 cagaaattaa aacgatttca aataccttgg agtgtaaatg gaattgctca aattgcagga
     7321 gttgcagcat taaaagatca agagcacata tgtaagtcaa aaaaaattat tcatagggaa
     7381 aggaattaca tgtataaaaa aattaatacc aaagttagcc acgctaatgc tttaagatct
     7441 gatgttaatt tctttttaat taaattaaaa aatatcaatt ctatcgcata ccaaaaaata
     7501 ttgttaaatc ttcacggcat ttttgtgagg gattgttcta gttttacagg aatgtctaat
     7561 ggatttataa gagtagctgt aagaactcat aaagacaata taatgttgtt aaaagcaatt
     7621 tatgatatta ataataattt gcataaatac aagttaatgg agaattaaat aattctacaa
     7681 agaaggaaga tgaagaattt ttatgaatgc taaagtattg atggttcaag gcacttcttc
     7741 aggagcaggg aaaagcacta ttgtaatagc attatgtaga attttctcta atttaggata
     7801 caaagtctct ccctttaaag ctcaaaatat gtcttcgaaa attcataata tttcaaatgt
     7861 ccacaaaata tctaagatac aagcgataca agcatttgca gcaagaaaac aacctgaatg
     7921 gcaaataaac cctattctat tagttccttt aggaaataat ataagcaata tctttgtagc
     7981 aggaaaattt ttagaagaaa tgtctgcaat aaagtattat aatgaatttg ttttggttaa
     8041 aggttttccc attgtattag atgctttaaa taaattgaga aatgaaaatg acatagtaat
     8101 aatagaagga gcaggttctc cagctgaaat taatattgca aaatacgata tagctaatat
     8161 gttacttgct gaaaaagtaa attgtcctgt aatattagta tctgatattg agcgaggtgg
     8221 ttgttttgct agtatatttg gaactataaa attgttaaaa ataaaacatc aacggctaat
     8281 caagggtttt attattaata aatttcgtgg tgatgagaat atacttgaat atgcgataca
     8341 atttattgaa cgcagaacta aattaaacaa tttgggtata attccaaaat ttgaattttt
     8401 tttgcctcca gaagattctc ttgatggtac aaataataat aataatctga caaatctaaa
     8461 caaatttagt tcaaaaagaa aattgaacga acaaatagat ttattagctg caactattgg
     8521 aaatagtata aatattgaat tcatcttgaa acaagtttta aaaatagaac aatgatatct
     8581 acttttcact taatcactat tctgctgctc tctatacttt tagatttgaa attaggagat
     8641 ccaaataata ggattcatcc cgttgcatgg ctaggaaaat acatcaatta ttttattccc
     8701 aagattaaaa atggtagaaa taaaaaatat gaaaaaataa atggagtaat atttactagt
     8761 ataactatta ctttttttgc catcatacta catatttcca tagtgtatct atatcatatc
     8821 acttttatct taatgatgat tttatccact tttattttgt tttctgtaat agcaataaaa
     8881 ggtatggaaa aacatatcac tgcaattact acagctttac aaaataatga tattgaaaat
     8941 gctaggataa atttgtctat gatcgtgagt agaaatactg aaagcctgga tgaacaacat
     9001 attttatcag gaacgataga atcgattgct gatagtacag tagatggaat tctatcacca
     9061 atattttatt tttctctgtt gggtcctaca ggtgctttta tttttaggat aatcaataca
     9121 ttggattcaa tgatgggtta taaggaaagt tattttgaaa acataggatg gatagcggca
     9181 aaggccgata cgtatgccaa ttatattcca tcaaggatta cggcaatatt aatgatattt
     9241 gctgcaatta ttactaacgc agactggaaa aattccataa ttacatttag gaaagaacga
     9301 aattgtaccg ttagtgtcaa ttctggtcag cccatttctg ttttagctgg agcactacga
     9361 gtaagattgg agaaattaga acattatagc ataggaaagc cactcgaaaa aatatcaatt
     9421 gaaaaatgca aaatggctat aaaaataatg aaaataacaa ttcttatttt ttgtgtgatt
     9481 attgccagtc cacttataat tattctgggt tatttaaatt ggtggaattt tatatttggt
     9541 atttaataat atatcttctg tcctttcttt tttaacaata attcccacta agagttacga
     9601 tataacattt atagctcaaa atatgtattt gttcccatta ataggactaa taatgggttt
     9661 agtaataggg gttttatctt ttttcttatt ttcttttcta caaggctttt tgctaggatt
     9721 tataataact gcaactatta taggaataac tggtcttcat catagcgatg cactatcaga
     9781 tttcgctgac ggattaatgg ctaaaggatc attcgagttt aaacacaaag taatgtcgga
     9841 tccacggaat ggtacagcag gaacggttat tctaattttc tatattattg gaatgataat
     9901 tttagtttca tatatagacg aaaaaccgaa attgatctct gctataatca tagctgaagt
     9961 tctatctaaa tacagtatgg ttttacaatg ctatctggga aactctgcat ggaaaggaat
    10021 gaattcaata tttataaaag aaatgaaaaa taaaaaaaaa ttcttagttt ccaatttaat
    10081 tttactggca agcataatta cttttggatt caatttttat tatatggtat tatctgtgat
    10141 aacaatgtta tcagttagta ttatgttatt attaatagca aatagatcat ttggtgggat
    10201 atcaggagat accataggtg caactaatga gatttcaaga tttttcattt atcttattca
    10261 cacaattttt taacaaattg acagataaag tttcacaaaa gttcaataaa aaaattattg
    10321 cagttattat ggctggtgga gaaggcagta gaatgaagag agattacaaa acagaaaaac
    10381 cactgataac gattaaaggg aaaaggttaa tagaatatgt aatcgaagct ctcataaaat
    10441 ctacttgttt tcataaaata gtagcttgtg taagtcaaaa tacttataaa acaatgaaat
    10501 ttttacaaga ttacaactat aactgcgaac agcctataga aattatagaa ggtaaaggta
    10561 atggatattc tagtgatgta tcttttatta ttgaaaattt ttttgaacat attatattta
    10621 tgatttcagc agatctacct ttactttcag atatagatat aataaagatc ttgagcaaat
    10681 gtaattttga tgctacctgt tattccatct tatttgataa aaaaattgtt gacgaaatat
    10741 gcataaaacc cagtgtagta tttgaatata aaaaaagaaa ttattgttat tcaggtataa
    10801 atatattcaa tctaacaaaa agacataatg atcagacttt aattggtgaa agatacataa
    10861 taataaatcg tattggaata gctgtaaatg taaatgaaaa aaaagactta cttgttgcaa
    10921 gaaaattaat taaaagttaa gatttaacaa atatgaagag actgtttata atcataatat
    10981 tagttaatcc aacgaactta gtaaatttcc ataaattttt gctttaccac ctgatttctc
    11041 cgctaacaat tcattacagg atttgcaata gatattagaa gtagtgtgag aataaattat
    11101 atttttttca tcacattttg aacactgaat attgagaaat gcactatttg gttttggtat
    11161 taatatattt tctttcataa tctaaacact tacttattta tgcaccaatt tctaattttc
    11221 gtatacgcat tccttctctc atcgatttgc gttgacaggt tttacaagta tatcttaaag
    11281 taatcttttt agttgtttta gcggttcttt ttaattcagg aaatttctga cctccgtaac
    11341 cttttttatc ttctgcatgc ctacgtgcgc ctaaagctgt tttcctatct tttcctttct
    11401 tatagataga tactgcttgt gttgtatggc tcttgcattt tgcacagaat ttgcgcattt
    11461 ccttctgcat cttcatagta atccttttgc gaaatagggt tatttaagga aaactaataa
    11521 ttcaattcaa actcataaat atatcttatg cttgttaaaa gaatagttta aaataagcac
    11581 atactctttt ttaagatata aaacacatct ataaccgtat ttttagaata atttaattgt
    11641 tatatgttta aacgcttata agcaaccgtt ttcttatcca ctacaggaaa atggacatat
    11701 ttggtaattt aatacaacag ctcagtatcg ggagtgtacc tattaatatg gatacattcg
    11761 gcgtatggat accattgacg tttggattag cagtaggttt agcatacgta atagcatctg
    11821 gtttaaggcg tccaacataa ataagtaaaa atttttagat accttttttc tttttgttac
    11881 ttgaaaaaaa gaaagtcgtg tgattatagg ctatttagtt tttgtttatt aagagcatcg
    11941 attcgtcact tttgtcaaat tctttcaaaa atgattttaa actcaatatc ggcacaacgg
    12001 gaactccttt tataaattta aagattgaat cattaagtgt tattatcata ggaagagctt
    12061 tagttatttt tttttctctt tttaaccaca acattgtcct tcctatttgt ttttcagcat
    12121 atattgtaag aggtgtttta ttgttataat tccaatgttt acagtctatg gtaattgcaa
    12181 aatcgttctt tatcccaata acgtctaact gggctctagg tttcgacaag acaatattaa
    12241 ccctcgtgtt gtattcaaat aaactcaaaa gttcacaagt aaaatattca aaatctttcc
    12301 acgacaatat tttagaacat tcttgaatat cacaaccaag ttgtagtgct agtagtgatg
    12361 ctctaatccg atccatctct gaaaataaga atacctgttc cccattttta tctaacaatc
    12421 ttccaattct gtttttacct agaaacttta ataattgttt gaatactgaa acactttcaa
    12481 tccctgtttt tgatctaaaa tcatgataat ttacttcatg agtcatccct tgaatagatt
    12541 ttaaaagaat acttggatta atataatgaa ccaatttata tggtaatata ttgtaggatt
    12601 taatttaaca acattcttct ataattacta tattatattc tgttgatttt gaagtctttt
    12661 ttgggtgctt gaatatttag caaaatctgc ttcagttcgt catcagttaa tgcccgatta
    12721 agtcttcctg ttgaagcggc atttataagg taattttcca cagcaccagc taattcgggt
    12781 ttgactattc taacattggc aagacgctgt cgtgctgatg gttctaaaag caccaataat
    12841 actctctgtc gcatagcttc agcttctgct tcccttcttc gcgtttcttc atcgttagaa
    12901 ttttggtttg gtatagacat ttttctttat taattaagaa tatctactca attcagggtt
    12961 tgttttttgt aactctttgt gaatctctgt tgctaaccta tcgactcttt tcattccatc
    13021 atcagatatt attcttccat tttttttctt aacaacatat ccagaacttt ccaattgttt
    13081 tagacctgtc cgtataattc ccccgctagc atcgacgtgg tgagcaagat tatatcctct
    13141 ttgtttgctt cctccatatt tacttcttag atcctcaaca ctgacgggtc catgaatata
    13201 cacttttctt aatattgatg cacatctaat ataccaccat tctttatttt gtggaatttt
    13261 ttcagtatgt ggaccagttt tagtaaaata tgaccatgca ggtggttcga tctttttatc
    13321 attttttaaa tacgtagcta atttttcaat aaacctatca gatggaacat cataaacttt
    13381 agccattaca gtatcacaat aattcttcaa cccctataaa tccttattct catcaatttc
    13441 gaacatccca tctcaaatca attttctata tacagtatta catgagagac atgttattct
    13501 taatgccttg attttggatc tacctattct taccgtgcaa ttgaatcccg gacaaatgaa
    13561 tagtttacag tttttacagt acaactgacg gatgtcataa ggaagtttta gtcttaatct
    13621 ttttgcaatt tttttagcga gttgggcttg ctcattggct aatttgtaat cttgacctat
    13681 ttcttgaaca gaattgtgaa gtaatatcac gattctttct tgtgctaata gtttcgcttg
    13741 atgttttccc aaaattatga tcgtatactt acgtttttaa aataaaaatt ttatgatcta
    13801 aggaagaatt gaagtcaatc agattaaaca tatatcatca aatattaaag acaataaagc
    13861 aaatagattc ctcttgacat cccacatttc tgtagtatta gcagaatctg cacttgaaat
    13921 tataccacca gaagtaagga atactcaatt aataaaaaat tattctaaaa aagttggtaa
    13981 ggagatatgt gaactttttc ttgacaagag ctaccactat agtgccatga aagaaaaaaa
    14041 tatagacttc atttggaaaa gaggtcgacc agatctagta catatatgtt tgttgtcgat
    14101 attgtcaact cctctcttct taaagaattt ggtagatgtg tatattcata ctatagacaa
    14161 caaagtaatt tttatcggac aacaagtccg tatacccaaa tcttatagac gatttgaggg
    14221 attaatgata aaattataca aagaaaagga gataaaatct aatgacaaag attacaataa
    14281 gtgtttatta aaaattgaaa aaaatctaac ttttgataac ttggtagata aggttattaa
    14341 accagataaa ataataggat tttcaagtac aggtattctc aaagacctca aaaatattgt
    14401 gaacgaaaat atagaaacaa ataataaaaa aatatctttt gtgataggtg gatttcaggg
    14461 tgggcatttt tctcaaagta caagagagag gtttcatatg acatattcta ttgcaaatga
    14521 acaattggaa gcgcatgttg ttatatctag attagtctat gaatgtgaaa accgtttatc
    14581 cttcttagat ggtccaagct aattttcaaa acaaagattt ttaaaaacag tttaatacag
    14641 taaatttatt ataaatgatt caaaacatga gttttatatt ctctatacaa ttaattgcat
    14701 ttattatttt gtttaatatt ttattacttt atccaattga ttatgctttc tctgatggtt
    14761 tatttgaaga aagattacct cctgcttctg taggtgacag ggaggctagt ctttatacta
    14821 aaattaatcc tccagtactt acttcagata ccaaagaaaa tgcatttttt gaattaagat
    14881 tatttgacgc aaaaactgac gaaaatatta aatttgtttc atattttata gcggttgaaa
    14941 aaaatgacaa gttattaatg agagatcttt ttcattctcc tacaggtcct ttaaagttaa
    15001 agattaatcc tacttctagt gaatctattt ctgtctttgg atctaccgaa ccttttcttg
    15061 gtggatggac tagtgagaca ggagatatta ccattaatgg tccgttactt ttacaaggag
    15121 ggctatatca ttttgcaata gaaatatttg gaatagataa tcctagaaat atatttacac
    15181 cagaaaatgc tccaaggttt gattcatggc ttagtgtagg tgatgtttat agagataata
    15241 taatcgacag tgaaaaaaaa tataatataa ctctaatatc atattatgac caaattcaga
    15301 actttaaata tgattctcaa caaagtaaca tgtcatggat aatgcctttt aattgggact
    15361 tgaaaagaat acaagacaac aatatttttg ttcatgaaga aataaagata cccaaaacat
    15421 ttacaaagtt tagtgaaaca aatgcttttg aagctgtagt taatggaaat cctttggttg
    15481 gaagatcaat agcattagat ccatttactg aagaaaataa ttttataatc catttattgc
    15541 tgaataaagc agagattata aaaattgctg aaacaataaa taatgatgct aaaaataata
    15601 atactaataa tacttctagc acaatgactt ttagtctttc tcctgctcaa caaaaaatag
    15661 ttgaaactac atcagaagtg gtatcagata ctggaggcat tctagtaaat ttcatttggg
    15721 atgaacaaat cttatccaat caagatacaa atctacagtt acgtttttcc gatgcgcttt
    15781 caactgaaga aataaatggt gatatcaaat atgatattat agtttacgat aaatcaggaa
    15841 ataaaattat tacaaaacaa gatttgatag cacagaacgc aacagattcc caggttatca
    15901 aatttccatc caaaggaatt taccaaatgg aattaaatat aaaaggtata acatatcctg
    15961 atttaggtac tcctgatgaa acaagaaagg gaatagcaag aggaactgta gtggtctctt
    16021 gacaaaatca atacagaaaa tatgttagat taatttaaaa ttgtccttta tagtgaaagt
    16081 gttaggttat tatagtttat atctaattta aaagatttat gaagattcaa tttgtcaatc
    16141 atctagtttg tttaaaccat tcagataaac agatccgttt aaaattaaat aatttaatta
    16201 gcaataatca gaatgaatgt attgaaggat ttttatattg tattaaatgt gatacaaaat
    16261 atcccattat tgaaggtata gcaatagttc taaaagacat gttagactac attcaaaata
    16321 ggacagaaat gtacggaaaa tggttattac attgtcagtc aaaagaaatg aaggaatatc
    16381 taaaggaaat cggaaaaaca ataactgttt ctaataaacc taatacctat gaagacggtg
    16441 gaatttggta tttaccttat aaatggttcc aacaagaaaa ccacaaagaa gatagattga
    16501 tgaaatcctt gaaaggaagt ccaaacccaa atgaacttta cgatcgtgtt ataaatagta
    16561 taaacccaaa gatagacgga atagctttgg atattggttg tgcaatgggt aaaactacgt
    16621 tgcaactcgc tcaaaaatat tcttttgtaa taggaataga tctttcattc tcattcataa
    16681 aggaagctcg taaatatatg caagaattta agcaaggaaa tgtggaattt tgtgtagctg
    16741 atgctgaatc tcctcccttt catccactga aatttgacct tacaatagca attaatctaa
    16801 tagagttggt gaatactaag aaactattat catcttgtca ttggttatta aaacctcaaa
    16861 gtgatattat aataacagat ccttatgatt ttaatagaga aagaatatta gacaaaacat
    16921 atgatgctca gtcatttaga accttaattg aagaaaatgg atttgaaata aacgaatcct
    16981 gtttaaaaaa agaagccttt attccttgga tattaaaaat aaatgaaaga gtttatttat
    17041 tctactttgt agactttatt aaagcaaaaa aaatctcaaa acataaagta attctaaata
    17101 aatactaaat tgaagatttg ttatatagaa taattaataa tattaagaac aaatagaaca
    17161 tagtagacca cgagatatgt atgagagtat tatttttaaa gatagaaaaa aactatctcc
    17221 tagatatata cctaaagaaa tccctcatag agagaaacaa atagaccttt taataagaac
    17281 atttttggat ataaaagaag atccagacaa atttccttta acgatcctac aaataattgg
    17341 tccagcagga attggcaaaa cgtcaacggt tataaaattt tctaatattt tggaaaatga
    17401 attaagaaaa agtaaaataa atattaaaat tgtgtacata aatttaaaat tacaaggcgg
    17461 aaataaatat gcaatttaca agtacctatt aagctgtatt gctccagaat tgcctgcaca
    17521 aggtcttagt gctgaagaga tgcttagaca aatgcttgat tatttgatta ttaataatgt
    17581 gtattctata gtcatacttg atgaaatcga ttacttgatt aaaattgcaa aagaaattgg
    17641 aataatttat gatctaacaa gattgaatga atttgatcct tctaaaaaat gtaatgtaaa
    17701 aggagtgatt tttattgcta gaagcactga attttatgaa aaattagacg aagctgaatt
    17761 aagttcaatg gggcgaacat acattgaatt tcctaattat acaattgaac aaataagtga
    17821 tattcttatt cgacgttcaa gagatgcatt tcaagaaaat gtaattggta cagatataat
    17881 tgattggata gcaaaaatag tagtttctcc tgttgtcaat ggagatataa gatatgcgtt
    17941 agatttacta tcatatgcag gaaacctggc agaatctgag ggaacagaaa gagtactaat
    18001 agaccatgtg aaaaagatta ataaacaaat ctataatgga attactgatg atgatattaa
    18061 agaattatca aatattcaaa ttattgtttt gctaggaata ataaagggat taaagattag
    18121 aaatagagat tatgtagatt taaaagagat tagaatgcaa agttcggaga tctctgaaaa
    18181 atacaagata agaaagttag atgtagaaga tatattagat gatcttgcca caagaaaaat
    18241 tataaaaata ctctctttga aaaaaattag tttgacatct tcctcaatag aaaacctaga
    18301 gaagatatta ataagtaaga tagattcaca tcctgattcc aaatgaatga tacctataac
    18361 gtcaaagata ttgtagaaca aggactagga agcataggta aaataaaaat attaagagca
    18421 ttagcagaag aaaaaaaatt attaactgtt tacgggttac ataaaaaaac acatctaaaa
    18481 agagaggata taaaaagaaa tttagctgag ctaataaaga ttgaatgggt aatagaacaa
    18541 aaaattagca atagtcttta ttcaattaat agagaaaata aatatatgca acgactagta
    18601 ttattttttt atgaaatagg atatatagaa aatatataat ttcaatactt atgatagatt
    18661 gatatggtaa taccaaccca ttaagaaatg atgattaatt ctcactaatt atcattatct
    18721 cctattagaa aaagtaaata atttggataa gcagaaaaat ttacccttaa aagtatagtt
    18781 aaaaaaataa gcgttttata acaaaaaaaa aaattggaat ttaattttga ttgatattat
    18841 agaatataat tgtataagat tcaaatatga aaccaacgct atatactatt ctatgaaact
    18901 gaaaaattat aaaacaacaa gaataggtca aatttttata tttgcactta ttggtatctt
    18961 atcttatttt acaattacca caatttcttt tgctcaagct caagaggaaa atttgaatgt
    19021 catagtaact ttttttgata ttactgatga tactggtgat gttataactt ttatcaacgc
    19081 tgatgatttt acaaaagcta aaatttttac ccccctcaaa agtactaaaa atggaaacac
    19141 cacagaactt caatttaatc tcactgctag ccaagtaagt atgggagaaa aatttcgcgt
    19201 atgcgcatta ttacttcaac aagacggtat aatatgcaaa gttggccaaa attcggaagc
    19261 agataggcct gaaataatag actttagact agcaactgat gacattgtta aaattgatgt
    19321 tgaaaatata tcagttcaag atttaggcaa taacaaaaat gataacgcca atgataatga
    19381 taacaaggac gacgatgaag ttgaagaaag taaagataat aacaataatt aggcaaatga
    19441 agatacaata taaataaacc tgtgaaagca ggttatttgt ttctatacta tagaaaaata
    19501 ataaatgcaa attctaattt agttatatta ttatttttac ttatttattt atttatttag
    19561 attagctata accattcttt cattctttgt ttttcgaaat tgattttttt cttataatat
    19621 agagttgatg gttcttttaa aattgtgatc ggaccattac tattgtaaaa attggtatca
    19681 ataaatcctg ctttcccatt tcctgtatcc ataaaacaaa atcctttacc atcgaactgt
    19741 actatctgat ggaattcttg tgaatcaatc cttattattt tatggataat ttgagaagca
    19801 acagatcttg cttgaccttc tgcgaaaata cctgcttttg ggatagatat aatatcgtta
    19861 attttaatct ctgtgacatc acctatagca aacacatcat taaattttgt ttctaaggta
    19921 gatttgttta ctgacatcca attacctttt tcctctatta atcctgattt tttaagcaca
    19981 gaaggtggaa aatgaaatgg gattccaact aatatatcat attccttttt tcgaccattg
    20041 tggaattcta aatcatattt attaacttct ttcagtgtat agtttggata taaatttata
    20101 ttttgtttct ttaatataag aattaatttt gaattaggtt gctctccagc aacaggtaaa
    20161 ggtaatttag atggaaaata tatatctata tctattttgt ttcttctatc aaccctacat
    20221 ataatttgat ctattataag cgctgattcg taaggtgctg gtggacattt atatggaaat
    20281 cccattatca caatagcaat ttttcctttc tctaaagaga gaattttttt ccttagacaa
    20341 ggtatacttt ctccatcata aagattataa ctattttgtt taataaatcc aggaatcata
    20401 cttgtagaca tttctgctcc aagagcaata acaagaaaat cataatgaag acgatcacca
    20461 tgatgaagag tttgtattag ttttgacttg gtatcaatgt aggttatttc atcttggatg
    20521 aatgtaatac cctttgattc taaatttttc aaggaaattt ttgaatcttc taaccttctc
    20581 ctcccatcaa gtatccataa atttgccaat cccatcataa aatagtcttt tttatcaata
    20641 actataatat catgagtaaa gtcaaggttt tttcttaaaa tatttgaaat acaaagtcct
    20701 gcgaaacctg ctcccagaat tattattttt ttacccatag tggagtgatt cttcttcctt
    20761 tattaataaa tattctatat ggcttgaaat tgagattgat attaaaaata tttatgaaat
    20821 ccaattgtat agttttaatt gaccttttat ttgtgccaaa atatattcat cctgctataa
    20881 acaattaaga tctatatcta gaattgggat atctcgctgt cagttaatcc ttataaatac
    20941 attactggta ctattacatc tatttgatat ttcgttttca atataagcta ttacattttc
    21001 tcaactaatt ctatatgaac atttttttct ctttgttatg tgatccctac taatagttat
    21061 accatgttga aaaatctaaa attcttattt aacatttata aataaaaata taaatatttc
    21121 actaaattca ttgttggtaa actttaagag aaggatataa gtaaatatat tttgtattcc
    21181 tattgtcatt tctaaaatat cggataagta gattaagaaa gtttacaagg aaccgtattt
    21241 atctcatatt tcgttatcta tttttcttat atgtaaaatt aaagttaaag ttaggatggg
    21301 tatagatata acaaataagt ttaatcataa ttttaaataa cattcaataa tggaggtaat
    21361 ggttagtata gcgatggatt ataacaagtt atgtaaatct atatttgctt tgcatgatga
    21421 cataagatat gcaggagtag tagatgaaac tggtgctcta cttgcaggag gaatgaagaa
    21481 aggtattgat tctatagtag atgaagcagg tgaagagctc tatttggccc aaacagctat
    21541 tagaagatcc atgagagaga agtttgatcc cacaatgggt aaggcaagat tttcctatat
    21601 tgaacgagag aaactctctg taattacgtt atatttggat aataaggtag ttttaataac
    21661 catggagcct aatattgatt ctcatacagc aatagatata gctgaggatt ctttagatat
    21721 gctagatggt aaaattgata tttaacaata atgaaattat tgtaagaata gattatctca
    21781 ttatttctta actattaaga aatagacaaa taaaaattca tcatataaga ttatgcatag
    21841 tttttaaatc ttgttcaaac tcatttaatt cggatggtac atttatagaa gtagcatctt
    21901 tgagtgacaa tggtttacct gtatttggat taattgaatt ttttttctta ttccaaacaa
    21961 gagaattaaa ctcaattatt ttttctgtat atttttcaaa atctttaaaa aaatcatttg
    22021 ttttggggaa tctctgtata tgaatacttt ctgaagaata aagagttttc cataattttt
    22081 ctgtaataaa tggacaaata ggcgcaacta ataataatat tattgagaaa catctatgca
    22141 aagtatatag tgatgatact tggaaattct tattttgatt atcatatgcc ctacctttaa
    22201 tcatttccac ataatgagac gcaaaaacat tccatgtaaa atctcttatg cttgttgcag
    22261 gtataaaaaa gttataatta tcatagcctt ttatacacgt atttataagt ttgtctaatt
    22321 cagataaaat ccacttatcc gtatgtaaaa gattattatt attttttaaa ggttcagtgg
    22381 aaaactcttt aggtacttca aaagaggaaa gaaacctacc taaattccat agttttgaga
    22441 gaaacttctg tgaattagaa atactttgtt cagaacaacg aaaatcctgt cccaaatttg
    22501 tttcactggc agaccaaaat ctaaatgtat ctgctccata tttttgaatt attggaaaag
    22561 gatcaacaac atttccttta cttttactca tcttttttcc tttttcatct acaccataac
    22621 ccattatcca aacattaggc catggtaatt tttctgttaa ttgataacat cgcagcattg
    22681 tataatataa ccaagttctt atgatatcct ttgcttgagg cctaattgtt gtaggatatg
    22741 ttctacaata aaattgggag tcttgagaaa atttggatat gaataaaggt gtaatacttg
    22801 agtccatcca tgtatcgaaa gttttttctt ctcctataaa ctcgttaaat ccacatttag
    22861 cacaggtatc aaaaggtggt ttttctttcc atggttgata atagttacct gactttggta
    22921 aattaggtga tttacaattt tgacaaaacc aaattggtat ctctgtacca tagaaccgtc
    22981 ttcgtgatat aggccagtct attgaaactg catttaacca attttttaat atttgtttat
    23041 gaacgggggg ataaaaattc aattttttag taaacgcttt gagaattgga atatagtcta
    23101 attgtttaag ataataatcc tgtaaaggta taatttcgat ctgagtctta cttctttcac
    23161 aaatgggggt tctatgcatg atgttttctt ctctttcaat aaaacttgta tttcttaaat
    23221 catcgataat tttttgtctt gcatgtttta atttcaaatt gctatatttt cctgcggaag
    23281 gagtagtata tccattttca tcaacggata taatttcttt gaggttcagt tctctaaaaa
    23341 cttgaacatc attatggtca ccataactac aaaccataac aacaccggaa ccaaaatcag
    23401 gttttgcaga atggtgggtt aaaattggga cctttctatt aaataaaggt attattacgt
    23461 cttttccaat taactgagaa tatctctcat cttttggatt gactattact gcctgacaag
    23521 caaaaagtag ttcagggcgg gtagatgcta ccactatttt atcattattg ttatcggcta
    23581 tactaaaaaa catataaatg agatttgtgg ggaggtcatc atagattatt tctgcatccg
    23641 atatggttgt accacaatca atgcagtaat tatttggcct attagaaata tagattagtc
    23701 cttcattcca taaatcaata aaggtttttt gggttaaagt tctgaattct attgaatctg
    23761 ttctgtaata ctctttaaaa ttactactta aaccgagatt tatcattatc tcgatcattt
    23821 ctttttcaag gtcatcaaga gcagttttgc ataattctag aaatttttct ctaggcacct
    23881 gtcgcattcg aactttgtat tttttttcag tatagatttc cactgggagc ccattcctat
    23941 ctattccaat aggaaacata acattgtgac cattcatcct tgcagttcta gcaatcatat
    24001 ctatctgagc atagtgtgca gcagcaccaa tatgccatgg tttccctgat gggtatggag
    24061 gaggtgtatc aataacaaat acgctatcac taccatcatt cttttttaag gtaaaagaat
    24121 aaagattttc tgaatcccat gactttaaga cttgtgattc aatttcgtga ttccaggatg
    24181 ttgaggtaat tattggttcc attctatatt ttacttctaa tattttatat ggttattaac
    24241 tttaatatct aaactataac caagagataa gatcggatat aaatataatc atcgcacatc
    24301 ggctaaaaga tgagctccct tattcacgcc ttcataaata tatacaccta atgcatcaat
    24361 atttttaggc atttttggag ctaaaatttt aagtatttct ttagaaaggt tctctactgt
    24421 tgcctcatct gataaaagat aaacagtaga tttgggcatt tgtaaatcaa aatatccctg
    24481 tgggccatca aatcgcacat aatagtaaag atcatcgtcc ttggatagat atctattatt
    24541 tacaaaaaat ttgtgatcta agtcattaat tgcttctttt atcaacattt tagcttcact
    24601 gaaatcaatg actaaaccat ttttcatttt accaatgatt tctaccatta cagttgatgt
    24661 atgtccatga aggatagaac atttttcaac taaaggtaaa atatgagcgt aatcaaatga
    24721 aagggaagga tcttcaataa aaatagatcc aattttttca tatttggtat caaagaaaaa
    24781 gattgattca aactcagtta atttgccctt atattttgaa gaccctattg caactatttt
    24841 taattctgga tagatactct ttattctgtt aaactttatc aaatcatcat tcgaatctat
    24901 aacttcaata tatttttcat catcagtttt aaaatctact gtaataactt ctccacctgg
    24961 aattttgatt tgagaattta cttcatactt tttaccaaga aaactcagta agttggataa
    25021 agataattga gttctattta atagataatc acctttatca ttaagatatt taatatcatt
    25081 ttcaacaata ttacgtatat ttgacaatat gagaaatgtg caaatctata tataaaaatc
    25141 taagcctttc atagataaaa gattaaatcc aaaatattat tctcaacaca ataatattgt
    25201 actttaaatg agttacgaac tgttatattc taaaaacgta aaattaaaaa aataaattat
    25261 aatacctctg aaatcacttt aaatgtcttt agatcttact aggataataa ttagttctat
    25321 taaaaattgc attttaacgg atgaaacctt tggcattgca ttttcaggag gaattgatag
    25381 ctccctactt gcaaaaattt ctagtgatat ttataaagag aatgttattt tattatcgat
    25441 tggttttcct ttttcacatg accttgaatt ctctagaagg atagctcaag aactaaatat
    25501 gaaacacttt acatatgaaa taaaaaacga ggaattcaat aatatatcaa gaaaggttat
    25561 tgacaatcta gaatgtaata acatttctca tatagaaaat tgtatagcat tttattttat
    25621 atcttttctg tcgattctga ataattgtaa acttgtttta acagccaatg gttttgatga
    25681 gttattttgt gggtataata gatatagaca aataatacta caaggtcagg aacagattaa
    25741 tagttttatg aaacaaagaa ttactaacga acttacactt gtaaacgaaa taaataatac
    25801 ttcaaaagaa ttcaatgtga agatcaaaca accatttttg actaaaagat ttattgattt
    25861 tgcaatgaaa atccctcttg agaaaaaaat actaggaccg tctgataatc ttaggaaaca
    25921 cattttaaga gaaattgcaa tttcgttaaa tgttccgata gaatctgcaa tgaaaccaaa
    25981 aaaagctctt caatatggaa ctttgatcca taaaaattta tcaaaaatta tcgatactga
    26041 taatgaaata aaaacaaaat tattgactaa aattaagcat taaatatttt tcttccaagt
    26101 ataaaggttc tatccaattt ccagattata aattatttgg atgtagacaa ttcattgcga
    26161 acagtcgaag ataccttttc gattatctta attccagagg caaaatgttg ttctggagta
    26221 aaaatgtcgt tcaattccat ttcggagaaa tattttgaaa ggacagaatc ctcctttatt
    26281 gcttgtatga attgcttacc tttatccttt ccttcaaatg ctactctctg tatatccatg
    26341 tatgcctcaa ttcttggaat cccttttcgt acaagggctt ctaaaatgaa ttcagcataa
    26401 atttgccctt ttgtaatatt tatatttgat gatatttttt caatatttac aaacatattt
    26461 tttacaactt ttatcatggt cgtcaacatt tcatccaaaa gaattattcc cattggtata
    26521 gtaaatcgtt cattagctga atttgataaa tcacgctcat gccataaaca aatattttcc
    26581 atagacacac ttaataaaga tcttaaaagt tttgctaaag aagatactct ttcactctta
    26641 atcgggttct ttttgacagg aactgcacta cttcccattt gcccgcttct aaaagattcg
    26701 gcaacttcat ctatctctgt tcgctgtaaa tttcgtattt caattgcaat tttatctaat
    26761 gaagtaccta tcaaagcaat aagaaattgt gcttctgcca atcgttctct tggaattact
    26821 tgtgttgctg cctctattgg atgtagatcc aacgttaaag cgaccatttg ttggacctcc
    26881 aatgctttat cacccattaa cgatccagtt ccgacaactc ctaaggtttt acatattaaa
    26941 aaacgctttt ttccttcttt tatcctttct atatgtttta acatttccat cgcccaaaca
    27001 gcgaatttta atccaaaagg aataatactt gcatgttggc catgtgttct tccaactgca
    27061 ggttgaattt tgaattctaa agctttgtta gataaaattt ttgctaattc tttaattttt
    27121 ggctcaatga ttaatagtga ggctcttaat tgcattgaag tagttgtatc gataatatca
    27181 ttactagtca aaccaaaatg tacccatggc tttgtattag gagaacactt ttcactcata
    27241 gcattgacta ctgcagccat atcatgatcg gtagtagatt ccaactcttt tgtcctggtt
    27301 aaagtgattt ttcctgattt ggcgagaaca ttgatctcat tggctgcgtc tattggaatt
    27361 attccaatct ttgattgagc ttgggcggtt gctgcttcaa attcaaggat aaagtcgagg
    27421 cgtttttgtt cttcaaaaat ttctttaata tctttgctac catatcttcc agaatctatt
    27481 gggagaataa ccattataag atattcaaat tatgaataga aataaatctt ttctatcgtg
    27541 tggttaaaag ataaccaagt atggttattc atcttttgta ataaagtcaa gaataagttt
    27601 ggcttgttta aaatgtacct catctatcat tctaccatca actttaaaag caccacgttt
    27661 atctccgtgt tcttgaattt cattcaatgt tgagataact tttcttgccc attcgatttc
    27721 attttgtgat ggaacaaaca cattatgaac aggttctatc tgctttggat ggatgatgct
    27781 tttacctgta taaccaagtc ttttggcagt tttagcatcg ccttgaagac catctaaatc
    27841 atctactttc tgccatattg aatctaaagc tggaattcca gcagcgcgtg catctacagg
    27901 tatttttgct ctagcataac tatattctaa tccctcattc tcataatcta atttcatatc
    27961 atacaaataa tcaaaaacac caaacaagag cgaacagact ctagaattaa attttgcaat
    28021 aagatatgaa tttactactc cttgagagga ttctatggat ggaatcaatc tgattatttt
    28081 ttttacgttt cgtttttttt ctaggttttc aattatttca gtaatttttt tcagttcaga
    28141 gtctgaattt acttttggaa ttacgatacc atccaacttc tcacaaatta cagattctaa
    28201 atcttgttca taaagtccag attcaaatga attaattcta acaaaaagtt gaggcgaatc
    28261 tttaaagtta taattcgaat cattaatttt gttgttatta ttattaatat taatattaac
    28321 aggatgtaaa ctattagtag aagaaaaaga ttggataatc atatctctcg ctattgattt
    28381 ttcagaaacc ggtacagaat cttcaagatc gaaacataaa atatctgggt aaaggagttt
    28441 tgatttctct agaaatctct tattgtttcc tggtataaat aataatgttc gaaacatcat
    28501 taaactatca caaattaaaa ttaataagta aaaaaaaagt tacagttctt gtgggttagt
    28561 taatatttta ccaatttgat cggcatttgc catcataaca tgacctttga ccatgtcaga
    28621 gaatggtaat ttcgtagcaa taagcggttt aatttttcct cttcctgtcc aataaattat
    28681 atcttctaaa tctgactttg ttccttgtgt agaacctaat agattagtac ctttaaagaa
    28741 caaaaatcga agatcaagtg tggtatcata acccgtggtt gcaccggttg caactaatgt
    28801 tccacccatt tttaataatg aaacctcctg aggaaaaact gcttttccga tatgttcaaa
    28861 gacaacatct acaccttgtt tatttgttag atttcgaacc tttttgttcc aatctggttc
    28921 tctatgattt acaacctggt cagctcctaa tttcatacaa gtgtccattt tttcctgatt
    28981 tccggcagtg gctataacgt cacaattata taattttgca atttgaattc cagccatacc
    29041 tacaccgctg gttcctccca ttattagaac ggtttgacct ggtttgatct ttgctcgtcc
    29101 tactaacatg tgccatgctg tcattcctac catagagata gcggctgcat cgtcaaatga
    29161 aacggaatca ggtatcttgg caacattaac ttcgggtaaa tgagtatatt gtgcaaatcc
    29221 accccataaa ggtccagttt ggaatcccca aatatttctt tcattacaat cgtattctct
    29281 tcctgcagta cacatctcac atatcctgca actgagattg gaatgtgaaa caactctatc
    29341 gcctgtccta aattttgtta catcttcacc tacttcaaca acaggtccgg caacatcact
    29401 tccagaaata tgtggtaaag gcgttttcac tggttccccc caaattgccc acaaatcatt
    29461 ataattatag gaggctgatt ctacctttat cataacttcg ttaggtttta attttggagt
    29521 atcaatatct tcgatcttaa ctacctgtgt aggatcttta ttatattctc taaaaacagc
    29581 tgctttcatg ctttggattt gcagcataga cttaatatag ttaacttgaa ataaagtaaa
    29641 aatttaattt tatcatcatt attgatttga aaattataat tataatttat tattataaaa
    29701 gtgaaatact actatgagaa attcgacaat actagtagat aaatagtttt ggttcacctc
    29761 agtttatctc aaaatctttt aaaacaaaat tccagttctc taatggaatt aattctagta
    29821 aatcctcttt ggtatcaatg tcaaatctga gcttgcctat atctattgat tctataggaa
    29881 ttttgaattt atgagcttct tttaaatggt tgatgtaact attatcatca taaaaagtct
    29941 tgataacatt tggtggtttt ctgaacaata cattggtacc atcaaatcgt ttggatgggc
    30001 atattataat acatttatga tatttatttg atatattaca aatttcatca atttctttgg
    30061 ctgaaataaa tggtaggtct tgtggaatta caatgttagc atttatatca ttattaatac
    30121 aataatgatc agctaatata actgcattat taacaccttc gtcgtaatcc ttaataaatt
    30181 ttagattttt taggcaagag tattttttaa cagctttatc actagaaact attatgattt
    30241 ccgaaatgaa cttagattct tttaactctt ttattgtagc atccaaaaga tacattgcaa
    30301 gatattttcg ttgttcgggt gttaaaatat ttgataatct ggatttggct aaatgaagac
    30361 ttttcatagg aataatagca gctattttca ttgataattc attaccttta aaatgaaact
    30421 tgctaatctc ttttcttctt ttgaattatt catcaaaata ttggtttcaa aagaagaaac
    30481 acctatttta tttatctctt tggagaaatt agaatctgag ttatctataa ctattttaga
    30541 aattaactgt gaataaaatt ttgctattcc taagacggaa gattctattc ctttagcact
    30601 aaggtatttt ctagctggcc cgcttattgc tttatttcct ataaatggag agattgcaat
    30661 aattttatcc ctttgttcaa tcaattcttc cttaatttga ttgagtgaaa gaattggtcc
    30721 tatacttgaa attggattag caggagcaat aataataata tcagatgatc ggatgattct
    30781 taatgcatct ttattgcatt ttgctttttc gctattctta taataaacat ctaatactct
    30841 tggttttgct ttatatttta cccaaaactc ttgaatattc atatctcctt tattcgtaat
    30901 tattcttgtt tctacatgcg tatctgaaac aggaattatt ttcttaatga tattgtattg
    30961 atttagtgca atccatttta ctactgttga caaagagtaa ccttttgaaa gcattttagt
    31021 tctgataaaa tgagtaataa ggtccttgtc accaatttga aaccatgttt cctcgcctaa
    31081 tagtttcata caattaagat aagaaaaagt gtcatttcta attccccatc cacgctcttt
    31141 atcaagtatg cctgctattc catatataca agtgtcaatg tccggacata catacaagcc
    31201 attaaaccaa aaattgtcac caatatttga aatgattgat aaattagaag tacaactggc
    31261 tagtccgcga ataattttta ttgaccctgt tcctcctgct aagactgtta tcaagtaatt
    31321 tttgtagtga ataaatgcaa tgtaaaattt tcgattatta atatattttt ttagtattaa
    31381 ttttatattt tcttctttta agatacaata aaaacaacaa taaggattat attcgacaaa
    31441 atgagattgt cagatgtgaa cagaatagtc gttttatcct gttggataat cgggttgatt
    31501 ttgattccta ctgctttttc tcaatcattt gcttcacaga tggaagtttt tcttttacca
    31561 gaaagcgatg cagctgatcc cgtctttact accattaggt ttgtaactat acaatatgaa
    31621 cctggaagcg aactagctaa aatattcaat ggaacaaacg agagaataac atttacttta
    31681 aattctacta ctcaaggtat ggatgaatta caaagctcta ttaatgaagc tattttgaaa
    31741 gataaaaaaa gtcctatcca gctttctaac tcaaaattag attatacagc acagataaga
    31801 ggagatgaag agagggctca aatatcttat aagataaaat ttgttccaca tattacaaat
    31861 tatgtacttc catccaatag cactactcca gcgcttgatc tcgattggag aagtgtggtt
    31921 gtacaaggac ctttaactgt aacggtgcct gattatggta aatttgatat caattatccc
    31981 attggtcccg ttaaagctct ctttccagct gcggctcaaa agttactatc tgtggaacag
    32041 accaaaaata tctttgaaac tcctcttcta gactatgaag aaattggttt cccaatggac
    32101 agatggcatt ttctttttga tccaactgga agtcaagctt ctgcagcagg ttcgggatat
    32161 gcagaagagg gaggatctaa agtagtatcg gtgttctcac ttggagaaag tagttttaga
    32221 gaaggaacac attctgctaa ggaaagttcg atatctacta ctatagatgg ttcaaaggtt
    32281 aacatcaaat catctgatcc accacccagc gcgcagatac aaatagcagg atttgcacaa
    32341 attaagaaga tcagtaatgc agaaattgct tttgttagtc caaaaggacc atcaggaact
    32401 gctactgcaa ctgggggatt cccactacag gtactcttaa ttttaggagg aatgatggat
    32461 gctgttgcta ttcttgtatt aattaaagct agaaagcctt agttatacaa gtattctcaa
    32521 caataattat acaaaccacc caccattttt gtacatttct ttatcgtctt atgtttttaa
    32581 agtaatgata aagacttta
//
LOCUS       DASVEV010000009         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_3544, whole genome shotgun sequence.
ACCESSION   DASVEV010000009 DASVEV010000000
VERSION     DASVEV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..302)
                     /locus_tag="VF222_00585"
     CDS             complement(60..302)
                     /locus_tag="VF222_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140953.1"
                     /translation="MHISNVFAQVITGGEEESVVYMQLSDILDNTQKDLDDLVQAINS
                     GEKDNALNITSNITTNIKEIENGLDLIVNNPIYGGD"
     gene            620..1078
                     /locus_tag="VF222_00590"
     CDS             620..1078
                     /locus_tag="VF222_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140954.1"
                     /translation="MAKVQHYRGLNTNLDILYNNVKQELQEQKDLEIISEYKGTLNSV
                     PLRSIVAVNKSPKVWIGSLREIHVSITGNPNDYAVEVASGAWFGSLVWTGAVGLVIGG
                     PIGLAAGATVGGIMAYEFEKHIWNKILEVVKKESEKQPTLDSIDHYYHSS"
     gene            1259..1582
                     /locus_tag="VF222_00595"
     CDS             1259..1582
                     /locus_tag="VF222_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140955.1"
                     /translation="MVEQSFAMWGCIPEITRDCIDMTNAGSTYLGIIGGAIIGAIITW
                     WVYNRQKKISEEQDNLLSHIKDVEENNELILKRVEYYQEHHEKLLKSLLSLEKKIDSV
                     LENNE"
     gene            1853..2038
                     /locus_tag="VF222_00600"
     CDS             1853..2038
                     /locus_tag="VF222_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140956.1"
                     /translation="MFTEKIITVNKDYYDKSNFKFILCDSCWWFATILKDVSKISNQC
                     PRCKKKKRMYVERITIY"
     gene            2159..2389
                     /locus_tag="VF222_00605"
     CDS             2159..2389
                     /locus_tag="VF222_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140957.1"
                     /translation="MALTNKYNNKSYYKDNLLFIISLIIISIGILFLIIGYGIYSPFQ
                     SLNHISSNDGLITIPKISHIDIIKDSSQYAAL"
     gene            2812..4062
                     /locus_tag="VF222_00610"
     CDS             2812..4062
                     /locus_tag="VF222_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140958.1"
                     /translation="MNTDYIFKNKLIDEKKLLFDIVILSIAIIIFSFFCLSDFKIIYS
                     DNQKEESEKIIGVNMKGMYTSLNYERFPNITISTDYYDQSFKLIKNIGMNHIRYVLYW
                     EAYENNPSLFLQELENVANIADKWGLKIIYDNHQYHTSSWLDEKRGTGYPSFLFDKSL
                     YPRNSGITTSSNSSELWWTNWWNRNIIDKNNTLDGWNLQANFLKTIVKLLDNYNSTYG
                     YEILNEPQIFSQDQWSKIGKYYKYMTNELRKQTNKTIVLDMTIPITFQDPAINLTSEN
                     MAKILPQDSNSIFKISLYGIPKDNNYQEKKLELLLNVSKIAKIPIYVGEWNHVNREKK
                     ATIANNTTDDTSKINASKSDLSQEESDFLVNKFNSLKVYGWAFWNWNYVNTPPQNFNL
                     INLTKNGDILPTKYFYILKNSINE"
     gene            complement(4688..4990)
                     /locus_tag="VF222_00615"
     CDS             complement(4688..4990)
                     /locus_tag="VF222_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140959.1"
                     /translation="MKRITAVLITASVLSLTAALLYIDNIDINKHAIAQITTQNPFPN
                     NTETIESLDKLLGNNTAIILNNTYISDPNNTLLDSININIKEDCMKLPNSEHMYCP"
     gene            5456..>6057
                     /locus_tag="VF222_00620"
     CDS             5456..>6057
                     /locus_tag="VF222_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140960.1"
                     /translation="MKLKKIKNRNFPNINNLIKIDFSLKREVLYVVVGAIVGAITMIL
                     PRTIYEFISGVPYYTSWIISGHVIGIYSSYAATAGFIVHLITAVSIGIVTGIFLYKTN
                     ILNISKLVNGILYGIFAGTIIFVVWAIPINEFILIPENIRTTTDIDSTSTEQEVSQQF
                     QSNKLNIFLNSLFINLLFGITVGLVSSFLSIKFGARYKCS"
BASE COUNT         2305 a          728 c          823 g         2201 t
ORIGIN      
        1 agatattcaa atagtaggat tgcaataaaa gtatatttta tctaataaat tactataatc
       61 taatctccac cataaattgg attatttaca attagatcta atccattctc tatttcttta
      121 atatttgtag taatattgga tgtaatattt aatgcattat ctttttcgcc agaatttatt
      181 gcttggacaa gatcatctaa atccttttga gtattgtcta atatatcaga tagttgcata
      241 taaacaacag attcttcttc accacctgta ataacctgag caaaaacatt ggagatatgc
      301 ataaaattac caccaacagt taggataaat atactaatta tgaaaatgac aacaggttta
      361 gtagcatata ccatggaatt atagtaagtt attaatattt aaatattcat gtaaaacgta
      421 aatatttctg aatctttatc attgaaaaag ctaataattt ttatcttgta gatgtataat
      481 atggacagta attacttctc agataatttc ataaagtctc ctatatttta atgactaaac
      541 tctatttact tatttgtaaa gtgttaaagt taccaacttt taatagttct tatatataag
      601 acttgtttaa cttattttta tggcaaaagt acaacattat cgaggtctta atacaaactt
      661 agatatactc tataataatg ttaaacaaga attacaagag caaaaagacc ttgagatcat
      721 ttcagaatac aaaggtactc tcaattctgt tcctttacgt agtattgtag ccgtaaataa
      781 atccccaaag gtatggatag gatctttacg tgagattcat gtatcaataa ccggaaaccc
      841 aaatgattat gccgttgagg tagcgtctgg tgcatggttt ggaagtcttg tatggacagg
      901 tgctgtagga ctagttattg gaggtcctat cggtcttgct gcaggagcca cagtaggagg
      961 aataatggca tatgagtttg agaagcatat atggaataag attctagaag tagttaaaaa
     1021 agaaagtgag aaacagccta cactagattc cattgatcac tattatcata gtagttgata
     1081 ttatttcata atgtgataat atttagaatt ttaaataaaa cttaacaata ttatattttc
     1141 tattcatatc tcatttaaat ttaaaattta tattcttatt caataaattt aatatggcaa
     1201 caaattataa aaaaactaat aattattatt aataaatatg agtacaaatt tattaattat
     1261 ggttgagcag agttttgcca tgtggggatg tattcctgaa attactaggg attgtataga
     1321 tatgacaaat gcagggagca catatttagg tattattgga ggagcaatta ttggtgctat
     1381 aattacttgg tgggtatata atagacaaaa gaaaatttca gaggaacaag acaatttact
     1441 tagtcatata aaggatgtgg aagaaaataa tgaattaatt ctaaaaagag tggaatatta
     1501 tcaagaacat catgagaagt tgttaaagag ccttctatca ttagagaaaa aaattgattc
     1561 ggttttagaa aataatgaat agaagaaata ttatatatgt tacattattc gatataataa
     1621 attcgattta ttaacattgt ttacttataa tttgccgata aacaatagaa aatggaaaga
     1681 attacaaatg taaaagagaa attaatctat ttttaatttt caaaaaaaat atcaatctga
     1741 tattacctgt aatgctaaac ggctaaactc taaagatttt gattctaaat caatttttga
     1801 tagattaaca aagttaacag aaacttatat ttcacattgc tgataaataa atatgtttac
     1861 agaaaaaatt attaccgtaa ataaagatta ttatgataaa agtaatttca aatttatttt
     1921 atgtgattca tgctggtggt ttgcgactat attaaaagat gtctctaaaa ttagtaatca
     1981 atgtccaaga tgtaagaaaa agaagagaat gtatgttgaa agaataacaa tatattaacc
     2041 aataatatta ttttttaatt aattgttttt tgtctgtaat taataataaa aaaagtgatt
     2101 aaattataag ttaacttggt ttactatatt ttaaatatta agttacataa taagaattat
     2161 ggcattaaca aataaatata ataataagag ttactataaa gataatcttt tgtttataat
     2221 atctttaatt attatttcta tagggatttt atttttaata ataggatatg gtatttattc
     2281 accattccaa tctttaaatc atatttcttc aaatgatgga ttaataacaa ttccaaaaat
     2341 aagtcatata gatataataa aggatagcag tcaatatgca gctttgtgac tgatacttta
     2401 aattgttctc ttataaattt agatccaata ttggttgata ttgatctgct attagatctc
     2461 taatttcata tttagtttat taataaaaat tcttgcattg taaaaggtta aatgcaaaat
     2521 tcattcaaat gatcacctct acataaagat agtgatatat tgtgcgtggt acttatgttc
     2581 tgatattttt agctatttgc ctatttattt gtcattctaa tctttagcaa tctaacatga
     2641 gttataattc catatattgg taaagttaac gtagttaact tataataata taattacaca
     2701 tttatatcta ttaaatataa aatgctcaat aacatacttt atttgtcagc atatgtagat
     2761 ctacctaatg aaatattcat tagacagtta tattattgat atattaaagt aatgaataca
     2821 gattacattt ttaaaaacaa gttaatagat gaaaaaaaac tactatttga tatagttatt
     2881 ttatctatag cgataataat attttcattt ttttgtcttt ccgattttaa gataatttat
     2941 tctgataatc aaaaggaaga atctgaaaaa atcattggtg taaatatgaa aggaatgtat
     3001 accagcctaa actatgaaag atttcctaat ataacaattt caactgatta ttatgatcaa
     3061 agcttcaagc ttattaaaaa tataggaatg aaccacatca gatatgtact ttattgggaa
     3121 gcatatgaaa ataacccctc attgttttta caagaattgg aaaatgtagc taatattgca
     3181 gacaaatggg gattaaagat aatttatgac aatcatcaat atcatacatc tagctggctt
     3241 gatgaaaaaa gaggaacagg atatccttcg ttcttatttg ataaaagtct ttatcctaga
     3301 aattcaggca taacaacgtc tagtaattct agtgaattgt ggtggactaa ttggtggaac
     3361 agaaatataa tagacaagaa taatactttg gatggatgga atcttcaagc taattttttg
     3421 aaaaccattg taaaattact tgataattat aatagcacat atgggtatga gatccttaat
     3481 gaacctcaaa ttttttcgca agaccaatgg tcaaaaattg gaaaatatta taaatatatg
     3541 acaaatgagc tccgaaagca aacaaataag actattgttc tagacatgac cattcctata
     3601 acgtttcagg atccagctat taatctaact tctgaaaata tggcaaaaat cctaccacaa
     3661 gatagcaact ctatctttaa aatttcacta tatggaattc ctaaggataa taattatcaa
     3721 gagaaaaaac tggaacttct cttaaatgtt tcaaaaatag caaagattcc aatatatgtt
     3781 ggtgaatgga atcatgtaaa tagagaaaaa aaagcgacga tagcaaataa tactacggat
     3841 gataccagta aaataaatgc tagtaaaagc gacctttccc aagaagaatc agactttctt
     3901 gtaaacaaat tcaactcatt aaaagtatat ggatgggcat tttggaattg gaattatgtt
     3961 aatacacctc cacaaaattt taatttaatt aacttaacaa aaaatggtga tatattaccg
     4021 acaaaatatt tctatatact gaaaaattca attaatgaat aagtttataa actaattgac
     4081 tgggaccctc ttaaaaaatt cttttatata caataatttt ctgcttacta attgcacaag
     4141 ttcaagaatt atctcataat tttttttctt tatttcatat catgtaaata atttcctaaa
     4201 tttctcatta tattatgaat ttattctctt tctacaatct atgtttttag atgatttctt
     4261 ttcttccagt ctatgttaac gtaagaacta agtgaatata atctaaacat aatgaaaaga
     4321 tacatttagt ttaattttca tcatacctaa attattgtgt tagtttttca tctttaaaat
     4381 taatgctaaa atgacttcca tcgcaaaaaa gtttattttt aagattcaca tctatataat
     4441 gctagtactg tttttccatc tttagaattt acaagatttt ctacgacttt gggttcttta
     4501 tctggtttgt atgtacttga atatctgttt gtcgatatcc gatcatatta taatattgac
     4561 ttaagccaat aaaactcgat actaagccag caaacattac aaatattttt atgaaagtta
     4621 tagggaacag aagataattg ataaataata taaataaaaa taaaaataaa aaaaaattat
     4681 gtaaggatta agggcaatac atatgttcag aatttggtaa tttcatacaa tcttctttaa
     4741 tgttaatgtt tatactatct agtaatgtat tgtttggatc agaaatgtaa gtattattta
     4801 atataatagc tgtgttgtta cctaaaagtt tgtcaagtga ctctatagtt tctgtattgt
     4861 taggaaaagg attttgtgtt gttatttgag caatagcgtg tttattaatg tcaatattat
     4921 ctatgtacaa aagagcagct gttagtgata gtacagacgc cgttattaat actgctgtta
     4981 ttcttttcaa tgtatcttcc agttgatatg gatctgtaag atttaaattt tttggttaga
     5041 tagacacctt catcataatt accaaattta ctattatagg aatattatat gatatacatc
     5101 ttaacgtcaa atctattttg aacttgtcat taaaattgta aagtgttctt cataatcctc
     5161 tacagataga ataatttcat cattattacg atgattgtat aaagtcttat tctaatccac
     5221 tttcttttta tgataatgtt tctgacgacc agatattaga ataacgatat tctaacgccc
     5281 gacaaaatgc agttcaatag attatagttt attttttatt ttgaataata aataattgtt
     5341 ctattactat cataaataat aaaattgtaa tttctaatat taaactaaga aattgagatt
     5401 atcaaatggt taaataaaat atcaaaaata attatataaa gaggatatag aagtcatgaa
     5461 actaaaaaaa ataaaaaatc gaaattttcc taatattaat aacttaatca aaattgattt
     5521 ttctctaaag agagaagtat tgtatgttgt agtaggtgcc atagttggtg ctataacaat
     5581 gattcttcct agaactattt atgaatttat aagtggagta ccatactata catcatggat
     5641 catatctggt catgtaatag gtatttattc ttcatatgct gccactgcag gatttatagt
     5701 tcatttaatt acagcagttt ctataggtat agtaacaggt atatttttat ataaaacaaa
     5761 tattttgaat atcagtaaac tagttaatgg tattctgtat ggaatttttg caggtacaat
     5821 aatatttgtt gtatgggcta taccaataaa tgaatttatt ttaattccag aaaacattag
     5881 aacaactacc gatatagatt caacatcaac agaacaagaa gtatctcaac aatttcaaag
     5941 caataaatta aatatttttc tgaattcttt atttatcaat ctcttatttg gaattacagt
     6001 tggccttgta tcttcttttt tatctataaa atttggtgca cgatataaat gcagtat
//
LOCUS       DASVEV010000010         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_780876, whole genome shotgun sequence.
ACCESSION   DASVEV010000010 DASVEV010000000
VERSION     DASVEV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780876"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VF222_00625"
     CDS             complement(<1..1715)
                     /locus_tag="VF222_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7140961.1"
                     /translation="MIGLSVLKNNISLLLIIIIIGIGSLSIISYQSASFTADEIKKIA
                     EEDIKSNARIQAYDLSRILVHSLDSINSNLKVLTSSPFLFENDIDQVQNLLYSAQSST
                     LNITDAYYWIDVTGRIVTSSKIYQTDKANAILDTQNQPFFLNPKKTLTPYYSTSITSQ
                     GSPSSFLYISYPLIKQQIVKYQNNTYNAKQIFNGVLVAAISVKSLGVFLQNEMTSSII
                     GNVGLMDRNGVIIYARNQSLIGNNYMSDEFQSLIPNDLRGSYNKIIKKGLEGKSLAED
                     LAYNGSTITLSYEPIVINEKYLWTLYISFPHQLASNVGSLIAQQNNFSTLIVVITGSV
                     TFGIIYLILSWNKKLEGEVNTRTGELKNTNISLMKSNELLEEANEQLKVHDKMQKEFI
                     NIAAHELRTPIMPILGEVEIIEEDLDPKTKTVKVEEEQIQLIIRNAKRLDRLASDILD
                     VTKIESNSLKLERIYFNLNDVLSNSIRDIQNQVSSGDIGTKNIKIVYEPVHIKISGDK
                     ERINQVVSNLLSNALKFTDEGFIKIKIENANDSVIISVEDTGSGIHPEIFPRLFCKFA
                     TKSDKG"
     gene            complement(1895..2395)
                     /locus_tag="VF222_00630"
     CDS             complement(1895..2395)
                     /locus_tag="VF222_00630"
                     /inference="COORDINATES: protein motif:HMM:NF028455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140962.1"
                     /translation="MDSNIFILDSSAFYAGFYRSFNWKFYTTTSILQEIKHILKSSLT
                     LDLLISSGALVIQDPTKESVDKIIYTANVLGDIKKLSQADISILSLAYQLNRILVSDD
                     YSVLNVSNFLKIKTISLGNEGIVALRKWKNFCKTCKKQYPPNIKECEICGNPTKRNYK
                     KFRPPV"
     gene            complement(2385..3206)
                     /locus_tag="VF222_00635"
     CDS             complement(2385..3206)
                     /locus_tag="VF222_00635"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX7140963.1"
                     /translation="MIKKIEGVAIITKNLNKTASEASEYIAKILSRKNIKVSSIPPFE
                     TSNFKNSSSTFTSLVNEIDLIIALGGDGTTLRAFRFHPSDIPVFSINVGGTRGILSEI
                     KLDQIDYAIDAILNGNYYYDERIRIQASLSNKLFLPALNDILIFRTNLTRTPTITIKF
                     RDGIISQRMDGIIISTSTGSTGHSVSLGGPILHEELNCLVINPIASINKLPSIIIPPE
                     EIEIYSNHKIDLIIDGQDKFLIEETNTIKITQFPINARFIRLRKKGFRQLEKLGF"
     gene            3393..3466
                     /locus_tag="VF222_00640"
     tRNA            3393..3466
                     /locus_tag="VF222_00640"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3427..3429,aa:Ile,seq:gat)
BASE COUNT         1165 a          504 c          517 g         1322 t
ORIGIN      
        1 cctttatcag atttagttgc aaatttacaa aataagcgtg gaaatatttc aggatgaatt
       61 ccacttccag tatcttccac actaataata acactatcat ttgcgttttc tatttttatc
      121 tttataaatc cttcatccgt aaattttaat gcattactta ataaattact aactacttga
      181 ttgattcttt ctttatctcc agagattttt atgtgtacag gctcatagac tatttttata
      241 tttttagttc ctatatctcc tgaagaaact tgattttgga tgtctctaat agaattagat
      301 aaaacgtcat taagattaaa atatattctt tccagcttta aggagttgct ttcaatcttt
      361 gtaacatcaa gaatatcaga tgctaatctg tctaatcttt ttgcattacg tattatcaat
      421 tgaatctgtt cttcttctac tttgacagtt ttagttttgg gatcaagatc ttcttcaatt
      481 atttctactt ctccaagtat aggcattata ggtgttctta attcatgtgc agcgatattg
      541 ataaattctt tttgcatttt atcatgtact tttaattgtt catttgcttc ttctagaagt
      601 tcgtttgact tcatgaggga tatatttgta ttttttaatt cgcctgttct tgtattgact
      661 tctccttcta atttcttatt ccatgacaaa attagataga ttattccaaa agtaactgaa
      721 ccggttataa ctacaattag agtgctaaaa ttattttgtt gtgctattag ggaaccaaca
      781 ttacttgcca actgatgagg gaaacttatg tataatgtcc ataaatattt ttcatttatt
      841 acaatgggtt catatgagag tgttatagtt gatccattat aagccagatc ttctgctaaa
      901 gattttcctt ctaagccttt ttttataatt ttattataac ttcccctcag atcatttggt
      961 attaaagatt ggaattcatc tgacatataa ttattaccta ttagtgactg gtttcttgca
     1021 tatattatga ccccatttct atccatcaat cctacattgc caataataga agatgtcatt
     1081 tcattttgaa gaaatacacc tagtgatttt acacttattg ctgctaccag tactccatta
     1141 aatatttgtt tggcattata agtattattt tggtatttta ctatctgttg tttaattaat
     1201 ggataagaaa tatataaaaa tgatgaaggg cttccttgag atgtgataga agtactatag
     1261 tacggagtca atgtcttttt cggattaagg aaaaaaggtt ggttttgagt gtctaaaatt
     1321 gcattagctt tatcagtttg atatatttta ctagatgtca caattctgcc agtaacatca
     1381 atccagtaat atgcatctgt tatattgagg gtagacgatt gtgccgaata aagcaggttt
     1441 tgtacttgat caatatcatt ttcaaaaaga aaaggtgagg atgttaaaac ttttaggtta
     1501 gaatttatgg agtcaagact atgaacaagg attcttgata aatcgtacgc ttgaattctt
     1561 gcattagatt taatatcttc ttctgctatt ttctttattt catcagctgt aaaggacgca
     1621 gattgataag atataataga taaggaacca attccaataa taattattat taataataag
     1681 gaaatattat ttttgagaac tgatagacct atcaactatt gacattatca tgtttatctt
     1741 agattaataa attaaatagg aataattctt atagaaaatt cctatataat attactcatt
     1801 ctttactagg atattgtaga taaacgtttg ttgaaaagat tcattcatta atcaatctaa
     1861 tataataaac aaaaaccatt aaagaatgtc tgaattaaac tggaggtcga aattttttat
     1921 aatttctttt tgttggatta ccacaaatct cgcattcttt gatattgggt ggatattgtt
     1981 ttttacatgt tttacaaaaa ttcttccatt ttcgtagtgc tactatgcct tcattaccta
     2041 aagaaatggt cttaatttta aggaaattag atacgtttag aactgaataa tcatcagata
     2101 ctaaaatcct atttaattga tatgctaaag atagaataga aatatcagct tgtgagagtt
     2161 ttttgatatc acctaaaaca tttgcagtat aaattatttt atcaactgat tctttggttg
     2221 ggtcttgtat tactaaggct cctgatgaaa tcaataagtc tagagttaaa gaggatttta
     2281 gtatatgttt aatctcttgt agaatagatg tagttgtata aaatttccag ttgaaggagc
     2341 gatagaaacc agcatagaaa gcactagaat ctaaaatgaa aatgttagaa tccaagtttt
     2401 tcaagttgtc taaagccttt ctttcttaac ctgataaaac gagcattgat agggaactga
     2461 gttatcttta tagtattagt ttcttcaatc aaaaatttat cttgaccgtc aattattaga
     2521 tcgattttat gattactata aatttcaatc tcctctggag gtattattat agacggtaat
     2581 ttattaattg aagctatggg attaattact aagcaattta attcttcatg taatataggt
     2641 cctcccaaag aaacagaatg accagttgat cctgttgaag tagatataat gataccatcc
     2701 atgcgctggc taataatacc atctctaaat tttattgtta tagttggtgt tctagtaaga
     2761 ttagttctaa aaattaatat atcatttaaa gcaggaagaa agagtttatt tgataaagaa
     2821 gcttggattc taattctttc atcatagtaa taattgccat ttaaaattgc atcgatagcg
     2881 taatcaattt gatccaattt aatttcagaa aggatacctc tagtacctcc aacattaata
     2941 ctaaaaacag gaatatcgct tggatgaaat ctaaaagctc gtaaagtcgt accatctcca
     3001 cccaatgcga ttatcagatc aatttcattt acgagtgaag taaaagtaga actgctattt
     3061 ttaaaattag atgtttcaaa tggagggatg gatgatacct taatattttt tcttgaaagt
     3121 attttagcaa tatattccga tgcttcagat gcagttttat tcaaattctt tgtaataata
     3181 gcaactcctt caattttttt aatcataaat catacgatat aacaatatta tagtcttaaa
     3241 tgcattttta ttatgtagtt atttcatata taatctatct aacaaatata ttgaaaacga
     3301 tgtgcagtgc cacaataata gattaagtga accaaaaaaa aatatttaaa ttgtctttga
     3361 aataaaacac caacactagt gtgtgctaaa ttgggccgat agctcagctt ggtggagcgt
     3421 tcgactgata atcgaaaggt cgagggttca actccctttc ggcccatgat tttaataata
     3481 caaacacaaa gccttaccgc aaagatat
//
LOCUS       DASVEV010000011         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1169193, whole genome shotgun sequence.
ACCESSION   DASVEV010000011 DASVEV010000000
VERSION     DASVEV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169193"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1459
                     /locus_tag="VF222_00645"
     CDS             545..1459
                     /locus_tag="VF222_00645"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7140964.1"
                     /translation="MDIHYDTVMQVISNEQIKKQRKPENTLGNQFLNKLYLDAIIDVD
                     SVIKDSQVRIWKSLKANPEFNISLNESQKVLEKFMESKIRLVILNIDLVGSTKMSLNL
                     SIDRLTTIIRAFAQEMTILLSLYGGYVLKYIGDAVLAFFVVDTTNTKDSYDDENKPTT
                     VSSGIDNFDSLSYTNVISCAYTMIKVIQEGINPILNQNDYPDLKVRIGIEFGEIAVVM
                     YGVDIDEYKNTILKRPHLDLIGYTISIAVKMTSHAKPDHIVIGEELFGKLHGKLKDTF
                     KQLSTNPDIWNYSTETTGKIYSLYENIR"
     gene            1866..>3280
                     /locus_tag="VF222_00650"
     CDS             1866..>3280
                     /locus_tag="VF222_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7140965.1"
                     /translation="MSYDNIDNGDNKKSSNNKETGKATLKVAEAEQRDIGRKIVRLDP
                     DAAQSLNVMTGDALELSSFGKNAVLLSWPGRDKDRGTGLVRIDGYTRNKLDVGIGDTI
                     EVKKVESKDAKSITLAPTEPLRIIGAEQYLAESLNGQLMSKGDIIPLNVMGQRIDLVV
                     ISTDPTGPIIINDATKVTVSEDSAKAVQASQEGKVPSITYEDIGGIRNEIARVREMIE
                     LPLRHPELFKRLGVEAPKGVLLHGPPGTGKTLLAKAVANETNANFYSIGGPEIMSKYY
                     GESEEKLRNVFEQAEKNAPSIIFIDEIDSIAPKREEVSGEVERRIVAQLLSLMDGLKS
                     RGKVVVIGATNRVNAIDPALRRPGRFDRELEIGVPDREGRLEILQIHTRGMPLAKDVN
                     LEKLADISHGFVGADLQSLSKEAGMRSLRRILPNIDLSSESIPSDTLRKIIVTMNDFM
                     DVIKEMEPSAMREVFVEIPDIR"
BASE COUNT         1238 a          453 c          564 g         1025 t
ORIGIN      
        1 acaaaaagta ctgattacaa tgtaaaatga cctaaactta tgataaaatc tgtattaata
       61 aatgtatata tacttcgcct aaatagacgt atggcatttt tattttccaa cttgaatata
      121 tgattgcagt ttttctcatt cccacgaact atcaaccata aatgagtctt aattattatt
      181 cttatttatt gtatatttac ccattgtttc ttattttcat atctatttct aggatgtacc
      241 tttaaatgaa attatggata taaatatgaa atataataag taattatttt cataagcata
      301 tattatttac cgtaaagctt tttacatcta tttgcaatgg aatattttcc taatataaat
      361 aatataatta cgataattat ataattatgc aaattaactg caattagaat taatcatata
      421 taataatatg tttattattt agataagatg atggtgattc aatataacgg ataagataga
      481 gcaaacaact actatagtca atttatacaa ttcaggaatt aatcccgaag taatagcatt
      541 agaattggat atacattatg atactgttat gcaagtaatt agtaatgaac agataaaaaa
      601 acaaagaaaa cctgaaaata ctttaggaaa tcaatttcta aacaagctat atcttgacgc
      661 aattattgat gtcgattcgg ttataaagga ctctcaggtg agaatatgga aatctttgaa
      721 agcaaatccc gagtttaata tttctctcaa tgaatcccaa aaggtattgg aaaagtttat
      781 ggaatctaaa atacgactcg taattttaaa tattgaccta gtaggatcta ctaaaatgtc
      841 gttaaatctg tctattgata gattaacaac aataattaga gcatttgctc aagaaatgac
      901 aatactacta tctctgtatg gagggtatgt gttaaagtat attggagatg cagttttagc
      961 attttttgtg gtagatacta ctaatactaa agattcctat gatgatgaga ataagcctac
     1021 tacagttagt agtggtattg acaattttga ttctctatct tacacaaatg taataagctg
     1081 tgcatatact atgattaagg taatacaaga aggtataaac ccaattttaa atcagaacga
     1141 ttatcctgat ttaaaagtaa ggataggaat cgagtttggt gagatcgcag tagttatgta
     1201 tggtgtagat attgatgaat ataaaaatac gatattgaaa cgaccgcatc tcgaccttat
     1261 aggatatact attagtatag cagtaaagat gacttcacac gccaaaccag atcatattgt
     1321 aattggtgaa gaattatttg gcaagttaca cggcaaacta aaagatactt ttaaacaatt
     1381 atcaactaat ccagatattt ggaattattc aacagagact acaggtaaaa tatatagcct
     1441 atatgaaaat ataagataaa tagcagttta cagtaaatta ttttcaaccc gaaagtttca
     1501 cgtataatta atttttttat tatttcgaat atggcccaca atataattac ccaattcatg
     1561 tcaaccaata aaatattcaa taaaaatttt gaccaaaatc tattaaaatg atatacttga
     1621 tgttgttcat ttaatgtaca tcttaattag aaacaaatga actccattta aagcaattaa
     1681 ttttttttcg attaaacttg taacaattgc atttgaaaca ataaaaggtt agattgaact
     1741 cgtaaataaa ctcaagtcat ttctataaca attatttgag atatttaagt acaattaaat
     1801 aattattaca tattcaaaca taaaatcatt aaataataca aagttaatac aaatattaac
     1861 aaaaaatgtc ttacgataat attgataatg gtgataacaa aaaatcttct aacaataaag
     1921 aaacgggaaa agcaactcta aaagttgcag aagcagaaca acgagatata ggaagaaaaa
     1981 ttgttcgtct tgatccagat gcagcccaaa gtttaaatgt tatgactgga gatgcattag
     2041 aattatcttc gtttggaaag aatgcagttt tacttagttg gccaggacgg gataaggata
     2101 ggggtactgg tttagttcgt attgatggtt atacaagaaa taaacttgat gtaggtattg
     2161 gagatactat agaagttaaa aaagtagagt caaaggatgc taaaagtata accttggctc
     2221 caacagaacc acttcgaata ataggtgctg aacaatatct tgcagagtct ctaaacggac
     2281 agctaatgag caaaggcgat ataatccctt taaacgtaat gggtcaaaga attgatctgg
     2341 tagtaatatc aacagatcca actgggccaa taattattaa cgatgctaca aaagtcacag
     2401 tttctgaaga tagtgcaaag gcagtacaag catctcaaga aggaaaagtt ccatctatta
     2461 catatgaaga tattggtgga atcagaaatg agatcgctcg tgttcgagaa atgatcgagc
     2521 ttcctctcag gcatcctgaa ttattcaaaa gattaggagt tgaagctccc aaaggtgttt
     2581 tattacacgg tcctccaggt actggtaaaa ccttgcttgc aaaagcagtt gcaaatgaga
     2641 caaacgctaa tttctattcc atcggcggcc ccgaaataat gagtaaatac tatggagaat
     2701 ctgaagaaaa attaaggaat gtattcgaac aggctgaaaa gaatgctcct tctattatct
     2761 ttatagatga gattgattct atagcaccaa agcgtgaaga agtatctgga gaggttgaaa
     2821 ggagaatagt tgctcagctg ttatctttga tggatggatt aaagtctaga ggtaaagtag
     2881 tagtaatagg tgctaccaat agagttaatg cgatagaccc agctttaaga cgtcctggta
     2941 gatttgatag ggaattagaa attggtgtcc cagatagaga aggtagactc gagatattgc
     3001 aaatacatac aagaggaatg cctcttgcca aagatgttaa tctagagaaa ctggcagata
     3061 tttctcatgg atttgtgggt gctgatttac aatctctatc aaaagaagca ggaatgagat
     3121 ctctgaggag aatccttccc aatattgatc tatcatctga aagtatacct agtgatactt
     3181 tgcgtaagat aatagtcact atgaatgact ttatggacgt tattaaagaa atggagcctt
     3241 cagcaatgag agaagtattt gtagagatac ctgatatccg
//
LOCUS       DASVEV010000012         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_393161, whole genome shotgun sequence.
ACCESSION   DASVEV010000012 DASVEV010000000
VERSION     DASVEV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393161"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..449)
                     /locus_tag="VF222_00655"
     CDS             complement(243..449)
                     /locus_tag="VF222_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140966.1"
                     /translation="MFDPNLNPLHNGYFATNSTGGFNEDTFAEPGLPGVHTMVFFDDS
                     NLNYRMDNEKNTYPVPITNKCPLE"
     gene            436..639
                     /locus_tag="VF222_00660"
     CDS             436..639
                     /locus_tag="VF222_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140967.1"
                     /translation="MGSNITQETREFGEKPFALILILIPSVGPQAEEIITVDSANTLV
                     SGKYMREKKRIDNTSEDINTIVK"
     gene            796..1257
                     /locus_tag="VF222_00665"
     CDS             796..1257
                     /locus_tag="VF222_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140968.1"
                     /translation="MKDLIIIILVLGLPLCLFSNTHIGIHQVNAQNNISSSSSSSLDT
                     SNTNENLNTEYKTYILIFGQRTIGNVDNSTKIVSSIVGHNLIKMEEEFLEEISLAPSQ
                     QLERQVQKVLNDGINGKQCGVNLTTQQEQKVVVDCISSGNTLIWYIYPTAK"
     gene            1749..1967
                     /locus_tag="VF222_00670"
     CDS             1749..1967
                     /locus_tag="VF222_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7140969.1"
                     /translation="MIICDPEYRFQVAHILEKMDNVVNVDVINGPYDLVVEIIFDTME
                     DLKVKIQNNIQNIDSLMSTLTLIRSDDM"
BASE COUNT          852 a          311 c          315 g          840 t
ORIGIN      
        1 tgtgataatg gtaccatatc cacatgtata tttacaattc attacgtctg aattgaagtt
       61 ctattattta tgaagaaagt agtacgagaa ctagagaata aataatatca attatcaatt
      121 ataattgtaa atagtataga aataataaaa agtttagaag atgtctacaa tagatgatat
      181 aataaattat ttacataaaa atacacctat tagattattt gtattagtta atctggttag
      241 tgttattcca atggacactt gtttgttata ggtactggat atgtattttt ttcattatcc
      301 atcctataat taagattgga atcatcaaag aaaaccattg tatgcacacc aggtaaacca
      361 ggttctgcaa aagtgtcttc attaaatcct ccagtactat tggttgcaaa atatccatta
      421 tgtagaggat ttagattggg atcaaacatt acccaggaaa cacgtgaatt tggtgaaaaa
      481 ccatttgcat tgatattgat attaattcct tccgttgggc cacaagcgga agaaattata
      541 actgttgatt ctgcaaatac gttagtcagt ggaaaataca tgagggagaa aaaaagaata
      601 gacaatacta gtgaagatat taataccatc gttaaataag tgaatgtcat actagaattt
      661 tgaattacta gttaatatag ttatagatat attgtatttc ttaagtacag cttttctaat
      721 atcaatattt acaaaactgt cattcccttt tattaacttc aacactttaa tactacaaga
      781 caatagtctg attccatgaa ggatttaata attataattt tggttctggg tctaccatta
      841 tgtctattta gtaatactca tataggaata caccaagtta atgcacaaaa taatatttcc
      901 tcatcatcat catcatcact tgacacatca aatacaaatg aaaatttaaa tacagaatac
      961 aaaacttaca ttctgatatt tggtcaaaga actataggaa atgttgataa ttctacgaaa
     1021 attgtatctt ctatcgtagg tcacaacttg atcaaaatgg aagaagaatt tttagaagaa
     1081 ataagtttgg ctccttccca gcaattagag agacaagtac agaaggtatt aaatgatggt
     1141 ataaatggta agcaatgtgg agttaatctc acaactcagc aggaacaaaa ggtggtagta
     1201 gattgtattt cttctgggaa taccttgata tggtatatat acccaaccgc taagtaattt
     1261 tttttaatac ttcttttctt ttccattgca attctaaaat ttatttaagg gatttttaag
     1321 gacgatatta tgatacggaa tgttattaac aagtccctac aattgatttc agttgttgtg
     1381 tatgtatgaa ccaataaatt aattatgact gtacctttct ccgacatttt ctaaattcta
     1441 aaaaattaat tatgaatcct ttcatattct cctgattagt tctctatttg tatctaaact
     1501 gagtgctaaa ttttattggt ttctctaact attctttaaa acacattata ttttgaattg
     1561 aatatttagg ataatcataa tatagagatt ttcaactatt cattataatg gtaaattccg
     1621 agtcataata caattccatt gtatctactt taccaactta ttcatttctt gttaattagt
     1681 atgtaaaaat tctaccattt cttttatata tttaataact atatagcttt atgctaaccg
     1741 catatctctt gattatatgt gatccagaat acagattcca ggttgctcat attcttgaaa
     1801 aaatggacaa tgttgtcaat gtagatgtca ttaatggacc atatgatctg gtagtggaaa
     1861 taatatttga tacaatggag gatttaaaag taaaaattca aaataatatt caaaatattg
     1921 atagcttaat gtcaactttg actttaattc ggtctgatga tatgtaaaca ccaacataga
     1981 ctagaataaa actaaaacta aaagataccg ttattaatca tattaatttt gatctcctac
     2041 aaccaatttg ttaccagaaa attatgtata tttatttaat tttttaattg ataatctgct
     2101 agattaataa tgcattaata ctatatattt tttttattac ctttattagg taattataat
     2161 agatggagag gataaactga gatttagctc tattactttt agttttattc aaagaaatta
     2221 aacattaaaa aagtatgcaa aagtcaaatt ctcggtgttt taccccttaa taaaaaataa
     2281 aactttgtta aatactatta ttaacattta atatcaag
//
LOCUS       DASVEV010000013         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1169776, whole genome shotgun sequence.
ACCESSION   DASVEV010000013 DASVEV010000000
VERSION     DASVEV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169776"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VF222_00675"
     CDS             complement(<1..1134)
                     /locus_tag="VF222_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7140970.1"
                     /translation="MDRKNSSFKIIYIILDGIGDLPHSSLNSRTPLEAASTPNLDYLA
                     TNGIMGEVITVGKGIAPQSDIAVFNMLGYNFTDVPYVGRGIVESIGCGIDLKNGDLAL
                     RGNFATIDENQKIIDRRAGRIIEKKEAQLLCDTLNSSISLKDPDASVVVIPTIAHRVT
                     IRFRHKKIGLSNQITNTDPAYDKVDGMGIAKTHISEMKVLKSIPEDNKISSKISSDIV
                     NDFSNQVYFISKNHAVNKAREEKNLNAMNCILVRDAGTTIPNYVQIDQKYNMKVACVV
                     DMPVEIGISKILGMEIIEAGSVNDYVTKAKVTAQELQKYDMIYVHIKGPDEFGHDGDA
                     LGKKQNIEKIDSDFFGTLLRELGISNAVIVVSGDHSTPCTKKSH"
     gene            complement(1219..1428)
                     /locus_tag="VF222_00680"
     CDS             complement(1219..1428)
                     /locus_tag="VF222_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140971.1"
                     /translation="MNNEDKINKKIVIAILSMPDGKQYEVPLVSKTFSSGREGFYAQI
                     PSLVYNNEVYGGQIQVWKKGDSKNK"
     gene            1592..>2055
                     /locus_tag="VF222_00685"
     CDS             1592..>2055
                     /locus_tag="VF222_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7140972.1"
                     /translation="MDFIECYRQAGRIACTIRELAKKKDYVGKTLYEICEQIEKEIQE
                     YGGFPAFPVNVSLNEIAAHYTAEPDDQKIVEEEDVLKIDIGVQVDGYIADTAVTISYN
                     NEYHSLVKASENALKEALKIARNKAKSSEIGKAIENSIIKSGYIPIKNLSGHS"
BASE COUNT          691 a          334 c          294 g          736 t
ORIGIN      
        1 atgacttttt ttggtacaag gagtagaatg atcgccggaa acaactatga ccgcatttga
       61 aattcctaat tccctcaaaa gagttccaaa aaaatcacta tctatttttt caatgttttg
      121 tttttttccc aaggcatccc cgtcatgtcc aaattcatct ggacctttaa tatgtacata
      181 aatcatatcg tatttttgta attcttgagc agtaactttt gctttggtta catagtcatt
      241 tacacttcct gcttcaataa tttccattcc taaaatttta gagataccaa tttctacagg
      301 catatccact acgcaagcaa ctttcatgtt atatttttga tctatttgaa cataattagg
      361 aatggtagtt cctgcatctc ttactaaaat gcagttcata gcattaagat ttttctcttc
      421 tctggcctta tttacagcat gattttttga tataaaataa acttgattgc taaaatcgtt
      481 tactatatca gaagatattt tagaagaaat tttattatcc tcggggatcg actttaacac
      541 cttcatttct gatatatgcg ttttggcaat acccattcca tctactttat cataagctgg
      601 atccgtattt gttatttgat tagataatcc tatttttttg tgtctaaatc ttatagtaac
      661 tctatgggcg atagtaggga ttacaacgac agatgcatcg ggatctttta atgaaatgga
      721 ggaatttaat gtatcacaaa gtaactgagc ttcttttttt tcaattatcc ttcctgctct
      781 tctatctatt attttctgat tttcatctat tgtagcgaaa tttcccctta atgcaaggtc
      841 cccatttttc agatcgatac cgcaaccaat agattcaaca attcctcttc caacatatgg
      901 aacatccgtg aaattataac ctaacatatt aaaaactgca atatcagact gcggagctat
      961 tcctttacca acagttatta cttcacccat aattccattt gtcgctaaat agtctaagtt
     1021 tggagtacta gctgcttcta acggtgtccg actattaagt gacgaatgag gaagatcccc
     1081 tattccatca agaattatat atataatttt gaatgaacta ttttttctat ccaagttcac
     1141 aatttagagg ttttttggta aatttatttt tatttaattg attaatagta attaaagaag
     1201 aaaagatatc ataaaatatt atttattttt actatcccct tttttccaaa cctgtatttg
     1261 tcctccgtaa acttcattat tgtagactaa tgaaggaatt tgagcataaa agccctctct
     1321 ccctgaacta aatgttttgc ttactagggg aacctcatat tgctttccat ctggcattga
     1381 caaaatagca attacaattt tcttatttat tttgtcttca ttattcatac cgatttcaca
     1441 tattgtataa aataatcata taaaacaatt tttatttgat gtcaactata taactacgta
     1501 ttttaaattc aatagttttt taaaaaaata aataaataaa gagaatatgg aagtttaaaa
     1561 caattttata tactataatg aaataattat tgtggatttc atagagtgtt accgacaagc
     1621 aggtagaata gcgtgcacta ttagagaatt ggcaaaaaaa aaagattatg ttggaaaaac
     1681 tttatacgaa atttgtgagc agatagaaaa agagatacaa gaatacggag gatttcctgc
     1741 ttttccggtg aatgtaagtt taaatgaaat tgctgctcat tatactgcag aacctgatga
     1801 tcaaaaaata gtggaagaag aagatgttct caaaattgat atcggtgtac aagttgatgg
     1861 ctatatcgct gatactgctg taactattag ttataataat gaatatcatt ctttagtaaa
     1921 agcatcagaa aatgctttaa aggaagcttt aaaaattgca agaaacaaag caaaatcaag
     1981 cgagattggt aaagcaatag aaaactcaat aattaaaagt ggttatattc ccattaaaaa
     2041 tctaagtggt cattc
//
LOCUS       DASVEV010000014        12433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_393282, whole genome shotgun sequence.
ACCESSION   DASVEV010000014 DASVEV010000000
VERSION     DASVEV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393282"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1039
                     /locus_tag="VF222_00690"
     CDS             332..1039
                     /locus_tag="VF222_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140973.1"
                     /translation="MVDHISRVFAHSFVTDYSSYLLALDKQIPVELELAKKNYPSNIT
                     LSMDHSDNAVTLLNKIYSAEDDIVEDNDLVKRYNKVTNSSNSTTYALVVADLLDETLR
                     QYADAIVTEIDLTNRSNLDLLDNMKNFTTSPSSESSSRINFTKFFSNNNPIFNYADYE
                     TAKALLVEIKCIFQSNLKSSNNVKNQSIDIISKLEKDLEKLSDLINNNGSPAKVIELV
                     HLQIHPSLQTGFGLQPK"
     gene            complement(1501..1608)
                     /locus_tag="VF222_00695"
     CDS             complement(1501..1608)
                     /locus_tag="VF222_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7140974.1"
                     /translation="MIYTKNGMYDDALKFYQENLEISKKVSDKQGMFPQ"
     gene            2242..3714
                     /locus_tag="VF222_00700"
     CDS             2242..3714
                     /locus_tag="VF222_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7140975.1"
                     /translation="MNNKIDIPEKERALVLQGGGSLGAYGAGAYGALYESISEMDTKN
                     GNKGKPVFDIVAGTSIGAINAAVLVSYVVENHTYEGSVERLIDFWKYLSKEAIVESNP
                     YFKQWWDYWHSINGEVASGEAARRYYSAKEFALYGVPNVFDPPQPRYDNKYYDSQNTW
                     YRFSNEPLKRSLERFAKFPIATTQEENQPRLILVAVDVADGMPVTFDSYPKEDGSRKT
                     EYGKFLRTKDKEIGFEHIVHYDKGITSDHVIASGSYPVNFDFSEIEVESRISQHSNRS
                     FDFENRTSSSKEPNHYEYRKELRHFWDGGLLSNTPLLQLVFLHRQYWYRVRRLKDSIP
                     RLGVCIVNLHPKKQEEIPMDRDGVINRNNDIIYSDRTHQEENMLLVVSDYIDIVKALL
                     KVSKDHGVKDVIINSLLNQKTRYHGEFLKVRTFRDIIEGRFQIDEIININRENDEHTI
                     SNKTYDFSVGTIKQLLELGYNDAREAYNEYIELSKRLKES"
     gene            3969..4946
                     /locus_tag="VF222_00705"
     CDS             3969..4946
                     /locus_tag="VF222_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEX7140976.1"
                     /translation="MCSVTRWSENGIAEVVARNMDWLEDLKSNIWVFPRGINREGSAG
                     KNSVKWTSKYGSIITSAYDICSTDGMNEKNFAGHLLWLAESDYGVRDENIPGLSLSLW
                     LQYFLDNFAYVNEAINYVEKYPLQILPVTAGNTGKIAQIHMMIEDTTGDAAILEYIKG
                     KLQVYHGREYIVMTNDPPFDKQIESLIQFKGFGGNKSLPGSTDSADRFTRGAYYQKNL
                     PKPNNIRETIAGVISVARNIAQPFGSPDQDRPNISSTRWRTVSDLTNGVYFFESTTSP
                     NIIWVFLRKLKFEKGSPVKKLDLVNEPDRIGDVSAEFQDSQPFEWLRPI"
     gene            5326..5898
                     /locus_tag="VF222_00710"
     CDS             5326..5898
                     /locus_tag="VF222_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7140977.1"
                     /translation="MTTINKSPQWMRGVQIGLGALTILLSIIAMLLPGLTMLSLVILI
                     AIVLVVTGIEKIITGLFISHKSRFLTVGLGILTVILAGVALTFPIIAAIAVLWILGFS
                     LMVDGFSRIADGVTNKSNRKWIRGFTVAVGVLAVIIAFMIINSPAFGAIFASIIISIA
                     LLITGIEMLTTGISGRKSSINGSSVGSDMK"
     gene            complement(6488..6781)
                     /locus_tag="VF222_00715"
     CDS             complement(6488..6781)
                     /locus_tag="VF222_00715"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7140978.1"
                     /translation="MRFLGGMMIGSLAGRHAARRQFEAMQAAQAQQEQINQAQTQAAQ
                     AQQMAKAAQTQQAPNPSKKNMVEELERLANLKQQGILTEEEFQQFKTKLLSDI"
     gene            complement(7412..7792)
                     /locus_tag="VF222_00720"
     CDS             complement(7412..7792)
                     /locus_tag="VF222_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140979.1"
                     /translation="MKFINEKQLKIFSLVSLSTIILGLYGSQIPVYGQDSNLEKANLT
                     NVPQKFSNVLNELGINSSELALKPGESVSVLIQKLQPNGTLYEFVQQFTDSVEKSGVN
                     VDNLTSFRQVENITQSAEELANLP"
     gene            complement(7838..8284)
                     /locus_tag="VF222_00725"
     CDS             complement(7838..8284)
                     /locus_tag="VF222_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140980.1"
                     /translation="MEYIKTIVFCALLLGSMSLISGTAIFGQLAIPSEQTLAIIGYDG
                     LLFGLSEVKTDERNLSVEIATPFVPNEDREFEAWLVDDKRGGSAYYLNLGVLNENGTL
                     NYNEHLMSPYTYTHLEITLEPANDLDPRPDTSNIVGTAELVPPFGK"
     gene            complement(9013..10836)
                     /locus_tag="VF222_00730"
     CDS             complement(9013..10836)
                     /locus_tag="VF222_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7140981.1"
                     /translation="MSTTNNFRPLGEDIYLNNARLLFKQDKWFIDDKSRFVLFRGVNF
                     ASRSKFPPYLPIVPLNKNNISENELDEEIKKMDVDIDRLKHLGLNIVRLLILWKGLEP
                     SPNPDIDKKLLPEGENYLNLMRKVIDALYSRNLFVILDFHQDIAHEIFGGDGFPDWAL
                     AIDDKDEIPQQPKTNNKIWAASYNLNKTVKNTLKSFWSNDLTNTRVGLKHFPVRSHLE
                     KTIGLTAKFFRDSSKSLSDLNDERGHPAIIGIEPFNEPHPVGIDKVHFEADLLKQFYF
                     NVLSEIRNYDNNLFLFMEPRVDWSVFSAEDINKSLSKSSSSRGFDLSRFDLRKFLKVG
                     DMNFIRTPLDGDIDKRGLIDTPKDINSYLPLKSDLNESFRNRGVFAFHYYDVRALASS
                     MLKIPKSIPQYKYDWPKVFRQLMEGATKRGLIPFITEFGGSHESEQIREYMNLLFIQI
                     ETNLLNSTYWNYDLYHTEQGKDNWNLEDFSLLGPNKRPRHIDVIARPYPMLSSAEPSF
                     LSFDIDSKYAIIILDGKVVEAPTIIYVPFHLHYSPTFYVWATSNEIKWDKEEQLLIWH
                     PSRERTKNQLIISIQPELNMKSMPVESKPLLEHTRFVNKFQ"
     gene            complement(10838..11407)
                     /locus_tag="VF222_00735"
     CDS             complement(10838..11407)
                     /locus_tag="VF222_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7140982.1"
                     /translation="MSDSQVVTAPKWLRIVQITIGVAAIILSIVIIVSPKLGGITAIS
                     MVAITLLIVGIERIASGISSRYSKRSRLINIGLGAVIVLFGIFTLANPSGSISFMILL
                     VGLALLINGIVRVVDALIKKKYEDNQANKIFRLIVGIISIAIAILTLASPGFGVILVA
                     LVVGIALLINGIEIIVAGIRGQSKRILGK"
     gene            complement(11774..12205)
                     /locus_tag="VF222_00740"
     CDS             complement(11774..12205)
                     /locus_tag="VF222_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7140983.1"
                     /translation="MSLEETKLLAWKFVDEIFNKGNINEADKLVTPEFIYHARGEDIE
                     GLEKFKEWFTSDTGVFEDIRFTLAESIAENGKVATAWMVEGTHEKEYSGVPASHKKFE
                     TVGISIFHFEGNKIKELWTVTDALTPALDLGIVKPVSSENK"
BASE COUNT         4385 a         1837 c         1855 g         4356 t
ORIGIN      
        1 ttctttaaca ttaacacaat gttaatattt ttaacttctt caatttctgg ttactattta
       61 caaaaaatca tagaatcaag cttcgtttat ccttatcact ttaacttcaa ctcaacatag
      121 cattattcag aatagcaaat gctttagtgg aaattgagta taacctatac aaattagctt
      181 tgaagatcga ttcacttgac gatgatatac ataacaattt tattataatt catcaattct
      241 tattctaaaa ttgaaaaaag ataatacaat tttaaattat tcatcaattt tttcaatttt
      301 tatcttagtc gtttttctta tttcattttt catggttgat catatatcac gtgtttttgc
      361 ccatagtttt gttactgatt attcatctta tcttctagct cttgataaac aaattccagt
      421 agagctagaa cttgcaaaga aaaactatcc atcaaatatt actttatcca tggatcattc
      481 agataatgct gtaacactat taaataaaat ttattctgct gaagatgata ttgtagagga
      541 taatgatctt gttaaaaggt ataataaagt aacaaactct tctaactcaa caacttatgc
      601 attagtagta gctgatcttt tagatgaaac attaagacaa tatgcagatg caatagtaac
      661 tgagatagat cttactaata ggtctaattt ggatttgtta gataacatga agaattttac
      721 aacttctccc agttctgaat cctcatcccg tattaatttt actaaattct tttcaaacaa
      781 taatccaata tttaattatg cagattatga aacagcaaag gcattattag ttgaaattaa
      841 atgtatattt caaagcaatt taaaatcatc taataatgtt aaaaatcaat ctatagatat
      901 tatttcaaaa ttagaaaagg atctagagaa actatctgat ttaataaata ataatggttc
      961 accagcaaaa gtaatagaat tagttcattt acaaatacat ccatctttgc agacaggatt
     1021 tggattgcaa ccaaaatgaa catgaatgga caaatgaaca tgtaatgagg taatttaatt
     1081 ttttatatag aataattttt ttatgttctt ttcatcatct gtgaattgac aggtaaatta
     1141 tattaaaata tattttcatt cgggttcggt gggatggttt acatatgtgt aaccagtttg
     1201 gtattaaatt tagataaaaa tttaatatct taattgtata ataaatagta attgaaagta
     1261 taaatcacat gtatatagtt actatgttat ataattaatt ttctattgaa gttattatat
     1321 taatttgcct cattcacata ttgtaaatca tgtgaatgaa aagctgaaat gtgaatgaat
     1381 caatggaaat tgttatcaat ttagtttgtt tgaaaattat tataacttct ttgaaataag
     1441 aaatattaaa attgaattta taaaatcatt ccatcaaatt gctcaaagta tttatgttat
     1501 ttattgtgga aacatccctt gtttatcact aaccttcttt gagatttcca aattctcttg
     1561 atagaatttt aaagcatcat catacatacc attttttgta taaatcacac ctaaaggaat
     1621 cacttacaga tctgtactat acctaaaatt gatgaattac tcgtaataat aaaattattg
     1681 atatttgata gatagattca ttcccaatag cttaaatata tattattgca tttgcacaaa
     1741 tcacacttaa acgagaaaat gtgttgagtc taaacttttc aacactaatt ttaaatcaat
     1801 aatccattta tggtcattat tagcttaact atataccaaa ggtttagaag ttattttcaa
     1861 ttcacatacc ggtaaaattg agtgaatttt ataaatctaa tatataaaaa ctttaatatt
     1921 aattaaataa cttcatcaaa taattcagag gttaacagaa acaatataaa gaaaatacaa
     1981 agaccaaaag acaaaaacct atgaaaagaa aagaattcta ttaatataat tatttctttt
     2041 gcgtaaatgt tttgatgaaa acatattcta ctttcttaat tagcgtatat tgtgatttcc
     2101 ttatatgaag tacatccgat aacaacataa aatttaaaat atgaatctag aacctaatat
     2161 tccttctaaa ttgaatatcg taaagtgtat gtaataaagt ttttttatct ttacatgtat
     2221 cttatcaatt aacattttaa tatgaataat aaaatagata ttccagagaa agagagggca
     2281 ttagtactcc aaggaggagg atctctaggt gcctatgggg cgggagcata tggggcatta
     2341 tacgaatcta tatctgagat ggatacaaag aatggaaata aagggaaacc cgtatttgat
     2401 attgtcgcgg gtacctctat aggtgctatc aatgctgctg ttctggtaag ttatgtcgtg
     2461 gagaatcata cttatgaagg atccgtagaa agacttatag atttctggaa atacctttct
     2521 aaagaagcaa tagtagaaag caatccatat tttaaacaat ggtgggatta ttggcattca
     2581 attaatggag aagttgcatc tggtgaagct gcaagacgat actattctgc taaagagttt
     2641 gcattgtatg gagtaccaaa tgtctttgat cctcctcaac ctaggtatga caacaagtat
     2701 tatgattcac aaaatacatg gtaccgtttt agtaatgaac ctctaaagag aagtctggaa
     2761 agatttgcaa agtttccaat cgccaccact caagaagaaa atcaacctag actcatactg
     2821 gtcgcagttg acgttgctga tggtatgcca gttacatttg atagctatcc caaggaagat
     2881 ggttcacgta aaacagagta tgggaaattc ttaagaacaa aagataagga aattggattt
     2941 gagcatatcg tacattatga taagggaatt acttcagatc atgttatagc aagtggctca
     3001 tatccggtca actttgattt ttctgaaata gaggtcgaaa gccgtatctc tcaacatagt
     3061 aatagatcat ttgactttga gaatagaact agtagtagta aggaacctaa ccattatgaa
     3121 tatagaaaag agttacgaca tttttgggat ggaggtcttt tgagcaatac tccgttattg
     3181 cagttagttt tcctacatag gcaatactgg tacagagtta ggagattaaa agattctatc
     3241 ccaagattag gagtctgtat agtaaatttg catcctaaaa aacaggaaga aattcctatg
     3301 gatagagatg gtgtaatcaa ccgaaataat gacattattt attcagatag gactcatcag
     3361 gaagaaaata tgcttttagt agtatctgat tatattgaca tagtaaaggc actattaaaa
     3421 gtttcaaagg atcatggagt aaaagatgtt ataataaata gtctcttaaa ccaaaagaca
     3481 agataccatg gagaattcct aaaagttcgc acatttagag acataattga aggacggttt
     3541 caaatagatg aaataataaa tataaaccga gagaatgatg aacatactat atctaataaa
     3601 acatatgatt tctctgtcgg aacaattaag cagttgcttg agctgggata taatgatgca
     3661 cgtgaagcat ataacgaata tattgaattg tcgaaacggt taaaggagag ttaataaaaa
     3721 aatgtttcta tattattagt taatatttct gttaattcta accatattaa ataaagacat
     3781 gataagtgta aaaactaaat tcaatataaa atatttcgtc atatttaaaa ttaagtgaaa
     3841 caacaaataa ttataaaata gcattcaata tcctaattca tgtagaaagt ggtattcatt
     3901 ctcacgtata ttgagatctt ctgaaatcga attgattata tggatcaaat agttctataa
     3961 atttctaaat gtgtagtgtt actagatggt ctgaaaacgg gattgcagaa gtagtggcaa
     4021 gaaatatgga ttggttagaa gatctaaaaa gtaatatctg ggtatttcca cgtggaataa
     4081 atagagaagg ttccgcagga aaaaactctg tgaaatggac ttctaagtat ggaagtataa
     4141 tcacctcagc ctacgatatc tgttcaacag atgggatgaa tgaaaagaat tttgctggtc
     4201 acctcctatg gcttgcagaa tctgattatg gagtgcgaga tgaaaacatc cctggattat
     4261 cattaagttt gtggcttcaa tattttctcg ataattttgc ttacgtgaat gaggctataa
     4321 attatgttga gaaatatcca cttcaaattc ttcctgttac agcaggaaac actgggaaaa
     4381 ttgctcaaat tcatatgatg atcgaagata ctacaggcga tgctgcaata ttggaatata
     4441 ttaaaggaaa actccaagtc taccacggtc gagaatacat cgtaatgaca aatgacccac
     4501 cctttgataa gcaaattgag agtctcatac agttcaaagg ttttgggggg aataaatctc
     4561 ttccaggaag taccgattca gcagatcgct ttacgcgagg agcttattac cagaagaatc
     4621 tacccaagcc aaataatata cgagagacta ttgcaggggt aatcagtgta gcccgaaata
     4681 tcgctcaacc atttggttcg cctgatcagg ataggccaaa tatatcatca acccgttggc
     4741 gaaccgttag tgatctaact aatggagtat atttctttga atctactact agtcccaata
     4801 ttatttgggt atttttaaga aaattaaaat ttgaaaaggg atctccggta aagaaactcg
     4861 atttagtgaa tgaaccagat cgaataggag atgtttctgc agaatttcag gattcccaac
     4921 catttgagtg gcttagacca atatagttac taataagatg ttttttggtt acatcaacag
     4981 gtgaacgaat cgacaatagg taaatagtaa tttcttttaa aacttattaa atttatttac
     5041 tcaaatatta ttccaaatta taagaatagt attttcatga ttaggtaata cagttttcta
     5101 tgtctattaa tataatgcaa atctaagaat attatttcaa aactaattca aatttaattt
     5161 atttaatatt caaataacgt tatagagata gtcaaatact atttttcgaa accattactt
     5221 gttagaaaaa ataccaatca attatgaaaa tcaataacga tcttaatagt tatgaataaa
     5281 aaaaatctgt atatatataa tacactattt aataagctag caataatgac aactataaat
     5341 aaatctccac aatggatgag aggcgttcaa attgggcttg gtgctttaac tattttactt
     5401 tctattatcg caatgctatt gcctggttta acaatgttat cgctggttat attaatagca
     5461 atagtattag tcgtcacagg aattgaaaaa ataatcactg gattgtttat ttctcacaaa
     5521 tctcggttct tgacagtagg actcggaatc ttgaccgtga ttttggctgg agtagctttg
     5581 acatttccta taatagctgc tatagcggtt ttatggattc ttggattttc gttaatggtg
     5641 gatggattct cgcgaatagc agatggagtg acaaataaat ctaacaggaa atggatcaga
     5701 ggatttacag ttgcagtagg agtattagct gtaattattg catttatgat aataaattcg
     5761 cctgcatttg gtgcaatttt tgcaagtata attatatcga tagccttgtt aattactggt
     5821 atagagatgc ttacaactgg aatttcagga cgtaaaagtt ctattaatgg atcttctgtt
     5881 ggtagcgata tgaaataact aattcactct cttcaagatt cctttttttt aactttaaat
     5941 tattttatta atcaaacaaa tatcaagatt tttttagtta catctatata tagacaatta
     6001 ttctgatcta attatcaatt tgatacagac aagaatacaa ggcgggcgta acaagattgc
     6061 ttattgtaat aaataatatt attaaatatg aataaactaa ataagtagaa taaagaatat
     6121 actaaatgca tctacgatac aataaaaaca atgagattta attggcacaa ataataacat
     6181 atagtcgatc aatctattag aaatttgact gtaatttatt ccctaatttt aatgacatat
     6241 agttagaata aaactcatga cttatatcat tggtataccc attgttcttc aaacttccta
     6301 gatattgaat tttattgttt ttttttattt taattatagt ttctaagtta ctatattgat
     6361 aaaattccaa acataattag agtgatcagc atatggtaat ttcttttcaa aagttggctt
     6421 ttctaataat ataatcagat taaaaaaatg gaaattcaat atttccaaac aaaaagcgta
     6481 tataacatta aatatcagaa agtaattttg ttttgaactg ctgaaattct tcctcagtta
     6541 atataccctg ttgctttaga ttagctaatc tctcgagttc ttcgaccata ttttttttag
     6601 aaggattagg tgcttgttgg gtctgtgcag cttttgccat ttgttgagct tgtgcagctt
     6661 gagtctgtgc ttggtttatt tgttcctgtt gagcttgagc agcttgcatc gcttcaaatt
     6721 gtctccttgc tgcatgtctt cctgctaaag atcctatcat cattccgcct agaaaacgca
     6781 taatatagat atcaatacga aatatttaat cttatatgtt agtatatcgt taacacatta
     6841 aaataatata ataatataat gtgtgagtta attaaatctt gtttattaat aaatattaag
     6901 agtttttaaa ttagattcat tgtttggtaa aaattaactg ttgaaatctc taccacttcc
     6961 ttgagaacag aagtaaatcc agttttaaca gtatatttta gaattccaaa acaagtgagt
     7021 ataatgagta tatcaagagg atggttatat ttgcacttat aaataatctt catccaaaat
     7081 tttgttaatg aactatttca attctttatt tagatagttc tagtatgaca gttacatata
     7141 atttagaatg ttctaataaa tccaattatt ttttttcaaa ttaaaaaaga attatttcgt
     7201 atatatgtta aaatttttca ctatttcatt ggatcaaaaa tttctcagaa tttaaaaaaa
     7261 ataacgcaat tataactata ataaaaatta tatcaaatta ttctaagatc aatataaaca
     7321 ggtgaatatt ttgcctatct ctcttctaat ctattaattt ttaattactt aataaaggtt
     7381 ataaaaataa tgaatgtttt attaaaataa attatggaag attagcgagt tcttcagccg
     7441 attgagttat attctctact tgtctgaatg atgttaggtt atctacgttc actcctgact
     7501 tctctacaga atcagtgaat tgttgtacga attcatataa tgtaccattt ggctgtaatt
     7561 tttgaattaa aacagaaaca ctttctcctg gcttcaaggc aagttcagac gaattgattc
     7621 ctaattcatt taatacattg gaaaattttt gaggtacatt cgttagattt gctttctcta
     7681 gattagagtc ttgtccgtaa actggtattt gagatccata caaacctaaa attattgtag
     7741 acaatgaaac aagagagaat atctttaatt gtttttcatt tataaatttc atttctatat
     7801 tattacctcc agaattgtta atatactttt cattttacta ctttccaaaa ggaggtacaa
     7861 gttcagcagt tcctacaata ttgctggtat caggtctagg atctagatca ttagcaggtt
     7921 ctaaggtaat ctctaaatgt gtataagtgt atggactcat taaatgttca ttataattta
     7981 atgtaccatt ttcgttaaga acacctaaat ttagataata tgcagaaccc ccacgtttat
     8041 catcaaccaa ccatgcttca aattctctat cttcattagg gacaaaaggt gtagctattt
     8101 ctacagagag atttctttca tcagttttta cttcgcttaa accaaacaat aatccatcat
     8161 agccaataat tgctaatgtt tgttcacttg gtattgctag ttgtccaaat attgcagtgc
     8221 cgcttatcaa tgacattgaa cccaataata gtgcgcagaa aactatagtt ttgatatatt
     8281 ccatattctt ccttgaagaa aatcggtata aaaagtttta tagagatctt taatatgata
     8341 aaaatgaaat tgtttctaat tgattttttt taaaggtatt aatattaaac atgctgtggt
     8401 actgtagtag tcaacactat actaattagt atttattttc taatcaagta gcggaatatt
     8461 ttagaattgt ggaacatttg atacttttga ataatcagat gtaatcttca atttaaattc
     8521 tgtttgaata ttaacttatt attttacttt taattggcga tggtgatctt tggatatatc
     8581 ttcgaaaact tatatagaat tacgatttta tattgttata caataatgtt gatttataaa
     8641 ttgggattat cataaatttg ttaataaatg acgagaacac tacacttgaa tttctctagt
     8701 taaaaacaaa gacaatagta aaagtaactg taacttgaat aatcttaatc ataagaaatt
     8761 taatttaatt ttaatcaaca ctttctaaaa gataatttgc aagtcactga ttaaaaaaca
     8821 ctggtatgaa tttgtttact attatggatc taacggacaa agagcttttc aaaatgacag
     8881 ctaaatatgg tttcctcggt acacaaaaaa tatttcataa agaattttga agtattaaaa
     8941 cttgttgatt aatgtatctc atttttgaat atttactcat atgcttttgt gtaatcctta
     9001 tttcaactaa aactattgaa atttatttac aaatcgtgta tgttctaaga gtggttttga
     9061 ttcaactggc attgacttca tatttaactc tggttgaata cttattatta gttgattttt
     9121 tgttctttct ctagatggat gccaaatcaa caattgttct tccttatccc attttatctc
     9181 gttacttgtt gcccagacat aaaaggttgg ggaataatgt aagtgaaaag ggacataaat
     9241 aattgtagga gcctcaacta ctttaccatc aaggataatt atagcatatt tcgaatcaat
     9301 gtcaaaagat aaaaatgatg gttctgcaga acttagcata ggatatggac gtgctataac
     9361 atcaatatga cggggccttt tatttggtcc taacaaggaa aaatcctcca aattccagtt
     9421 atcctttcct tgttctgtat gataaagatc ataattccaa tatgttgaat tcaatagatt
     9481 agtctctatt tgtatgaaga gaaggttcat atactctcgt atttgctctg attcatgact
     9541 tcctccaaat tcggttataa atggaattaa tccacgtttc gtcgctcctt ccattaattg
     9601 tcggaacact ttaggccaat catacttgta ttggggaata ctttttggga ttttaagcat
     9661 tgaagaggcc aatgcacgga catcatagta atgaaacgca aatacgcctc tattcctaaa
     9721 actttcattc aaatctgact ttaagggaag ataactgtta atgtcctttg gagtatcaat
     9781 gagtcctcgt ttatcaatat cgccatcaag gggtgtacgg ataaaattca tatcaccaac
     9841 ttttaaaaat tttcttaaat caaaacgaga taaatcaaat cctcgtgatg aggatgattt
     9901 cgagagagac ttgttgatat cttcggctga aaaaacagac caatctactc ttggttccat
     9961 aaacaaaaat agattattat cgtaatttct tatttctgat agtacattaa aataaaattg
    10021 ttttaaaaga tcagcttcaa aatgaacttt atctatacct actggatgtg gttcattaaa
    10081 tggttcgata cctattattg caggatgtcc acgttcatca tttaaatcag acaatgattt
    10141 tgaggaatcc ctaaaaaatt ttgcagtcaa acctatagtc ttctcaagat gagaccgaac
    10201 aggaaaatgt tttaatccaa ctcgtgtatt tgtcaaatcg tttgaccaaa atgattttaa
    10261 ggtatttttt acggttttat tgaggttgta tgatgctgcc caaatcttgt tattcgtctt
    10321 tggttgttga ggtatttcgt ctttatcatc tatggctaaa gcccaatcag gaaatccatc
    10381 tcctccaaaa atttcatgag ctatatcctg atggaagtcc agtattacaa ataaattcct
    10441 agaatataac gcatcaataa ctttcctcat taaatttaaa taattttctc cttcaggaag
    10501 taacttttta tcaatatcag gatttggtga gggttccaat cctttccata aaatcaataa
    10561 tctaacaata tttaatccta gatgttttag acgatcaata tcgacgtcca ttttttttat
    10621 ttcctcatct aattcatttt ccgaaatatt atttttattt aaaggtacaa tagggagata
    10681 aggaggaaat ttacttcgtg aagcaaaatt gacgcctcta aagagaacaa atcttgattt
    10741 atcatctatg aaccatttgt cttgtttgaa cagaagacgt gcattattaa ggtatatgtc
    10801 ttcacctaat ggtcgaaaat tattagtggt actcatatca tttacctaaa atacgtttgg
    10861 attgacctcg gatacctgct acgataattt ctatgccatt tataagtaaa gctataccta
    10921 ctactaaggc aactaaaatt acaccaaagc caggtgaagc taaagttaat atggcaatag
    10981 caatagatat tattcctact attaaacgaa atattttatt cgcctggtta tcctcgtatt
    11041 tcttcttaat taaagcatcg acaactctaa ctattccatt gattaagaga gcaagcccta
    11101 ccaaaagtat cataaaacta atactaccac taggatttgc aagagtgaag ataccaaaga
    11161 gtacaattac cgcaccgagt ccaatattaa ttaaacgaga tcgtttacta tatcttgagc
    11221 ttatcccact tgctattctc tcaatgccaa caattagtag agttatagct accatagaaa
    11281 ttgcagtaat acctccaagt tttggggaaa ctataatgac aatcgataga ataattgcag
    11341 ctactccgat agttatctga acaatacgca gccatttagg tgcagttaca acttgtgaat
    11401 cagacatgat atatttaatt ctccataaat ttcaacaaag acttattccc aacatatata
    11461 tttaagtatt tttaaataaa agagaatttt ttatgaagat atttattatt tctattaatt
    11521 ttctaaataa ttaaaatatc aattgtgaga taaaacttca aatccctagt ctataagtta
    11581 aaacataact tatattttat gaacttagac tctcagagtt accgaaaata ggatcttgca
    11641 ttctgacctt atacgagtta tttcaaattt acatttcaac aatttagtaa tgctgaggaa
    11701 atttggtaaa gaattgtatc atataacatc aatagtagaa ttggcaagtt ctccaggtta
    11761 aaaacctatg gagttatttg ttttcagaag aaactggttt aacaatacca agatctaatg
    11821 ctggtgtaag tgcatcagtt actgtccata attcttttat tttgttacct tcaaaatgga
    11881 aaatactaat tcctacagtt tcgaatttct tatgactagc tggaacaccg ctatattcct
    11941 tttcatgagt gccttccacc atccaagcag tagcaacctt accattttca gctatacttt
    12001 ctgctaatgt aaaccggata tcctcaaata caccagtatc tgatgtaaac cactccttaa
    12061 atttttctaa tccttctata tcttctccac gagcatggta aataaattca ggtgtgacaa
    12121 gtttatctgc ctcattgatg tttcctttgt taaatatttc atcaacaaat ttccaagcaa
    12181 gtagtttagt ttcttctaaa gacatactaa aatctatacg cctgttttga tttaaatata
    12241 tctatttggt ctaaagatct tataccaaat ctcttatttc gctttctttg atatctaaaa
    12301 ttctagtctt tcatcctgac cgtggaatac aaactgcagt acatttctaa agtacattcg
    12361 taaaattaga ttattataac ttaacaaact catcagctta ataaaaatct agtaataatg
    12421 atctggatac ata
//
LOCUS       DASVEV010000015         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_781773, whole genome shotgun sequence.
ACCESSION   DASVEV010000015 DASVEV010000000
VERSION     DASVEV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781773"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF222_00745"
     CDS             complement(<1..201)
                     /locus_tag="VF222_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140984.1"
                     /translation="MLNKNNLTKFSSKFTIVIVTFLISAIFLMPFKESHGLEDNLTIT
                     HGVASGDVTKNSTIIWSKSNAQS"
     gene            886..1452
                     /locus_tag="VF222_00750"
     CDS             886..1452
                     /locus_tag="VF222_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140985.1"
                     /translation="MSTETRKIGNSQGYWGNRFILSAIIQGAVITILTLIIVGFQLTI
                     SNINIIQYLSLTFDGPSKWFFLGYILYLILIVAIAVTAVFYLHLEIHMNKKITGLKSV
                     LAGIHLLGMNIGGAGTTIVMIYAGLAGSGILDIVLKGTTSGLGSTINSNVDIMVQFIL
                     PIAGFAGLLSIGVISGGLTYIMTFFQKT"
     gene            complement(1469..2041)
                     /locus_tag="VF222_00755"
     CDS             complement(1469..2041)
                     /locus_tag="VF222_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140986.1"
                     /translation="MNKKAELKGSSGRENDKLNEKVIKETVKWSITEYSLFVSKLVKK
                     GLSLFNEENLENGWFSGIYVNNQGEIDFTDPFTMEKRPHNNINIIGAVGSFNLNDSQE
                     YYVIPETNNKFVEVLDHKGEIDTDFPLKDYRLRLNNVSSNSKYSNKASIRKYDNAVSF
                     FITNEIQKGSFLDLFEIYIEKKINILNKCG"
     gene            complement(2231..>2417)
                     /locus_tag="VF222_00760"
     CDS             complement(2231..>2417)
                     /locus_tag="VF222_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140987.1"
                     /translation="GKLLTGGQFADKRGHFFVIEAADELELLELLNRNILDSCKVESH
                     PIVSFEDLFGFFERNPV"
BASE COUNT          875 a          324 c          310 g          908 t
ORIGIN      
        1 agattgtgcg tttgattttg accagattat agtactattt tttgttacat ctccactagc
       61 aacaccatgt gtaatagtta aattatcttc taatccatga gattctttaa atggcattaa
      121 aaaaatagca gatattaaaa acgttacaat aactatagta aatttactac taaatttagt
      181 caaattattt ttatttagca ttaaatatca taattcaaca atgattcaga gtataaaaaa
      241 gtttttctaa aaatatcttt tttgttatca tatttgaatt taaatcttat aatttaactt
      301 aattagtcac atttaatttc tactagaagt taaagtttta cataaagtcc aaattaacac
      361 actgataggt taccaacaga ggctaaagtg agaaaagttt gtcataaatt tataataaat
      421 attgattatc ctttaactat gaaatctcca atagtttatg atgtaaattt agagttctca
      481 tgaaaaaaaa tattgtgata tataaccagt aattatagaa attaataagg aacgtgttat
      541 agcaagatgg aaaaaaagaa atatctttca aggccaatca tttagacttg agtggaagta
      601 aaaatatata atctaaagaa aaatgatttt cttcaaataa tacaatcaat tataaaagat
      661 tagtaacgaa tgtgataaca tttttcagca ttgatattat taataataat aataataata
      721 ataataataa taattattag caagtgaata acacaataat cactcactat cctttttcat
      781 aaatccttgt gtgtgcatat gtagtaatct cttattttaa attattcaag aattaggttt
      841 aattttcaac taaatattct tatatataga tttatcaaaa gaatcgtgtc gacagaaact
      901 agaaaaatcg gtaattcaca aggatattgg ggaaatagat tcattctttc agctataatc
      961 caaggtgcag ttataacgat attgacattg ataatagtag gttttcagtt aacgatttct
     1021 aatattaata ttattcaata tctttcattg acttttgatg gtccatctaa atggtttttt
     1081 ctaggataca tactttatct tatattgatt gtagctatag cagtaactgc cgtattttat
     1141 cttcatcttg aaattcatat gaataagaaa attacaggat taaaatcagt tcttgcaggc
     1201 atacacttac tcggcatgaa tattggagga gcaggtacta caatagttat gatttatgca
     1261 ggattagctg gttcaggtat attagatata gttttaaagg gaaccacctc tggacttggt
     1321 tccacgataa attcaaatgt agacataatg gtacaattta ttctgccaat agccggattt
     1381 gcaggtttac taagtattgg tgtaatttca ggaggattaa catatataat gacttttttt
     1441 caaaaaacct aaagatatta aaattatttt aaccacattt atttagtata ttgattttct
     1501 tttcaatata tatttcaaat aaatctaaaa acgagccttt ttgaatttca tttgttataa
     1561 aaaatgagac agcattatca tattttctta tacttgcttt attagaatat ttagaattgg
     1621 aagaaacatt gtttaaacgt agtcgataat cttttaaagg aaagtctgta tctatttctc
     1681 ctttatgatc taatacttct acaaatttat tatttgtttc tggaataaca taatattctt
     1741 gagaatcatt aagattgaat gatccaactg ctcctataat gttgatatta ttatgaggtc
     1801 ttttttccat agtgaaagga tcggtaaaat ctatttcgcc ttgattatta acataaattc
     1861 cagagaacca gccattttca agattctctt cattaaataa acttagacct ttttttacca
     1921 gcttgctaac aaataaagaa tattctgtaa tactccattt aacagtttcc ttaatgactt
     1981 tttcatttaa tttatcgttt tctctaccgc tgctgccttt aagttcagct tttttattca
     2041 accttgtact tgttactagt tcattttata tattaagggg ttggtgttct ataactaaat
     2101 agaatttatt tataaaatag atgttaatct acataaagat tcattatagt atataataac
     2161 agtttaaata tttgagattg ataatacgac cgttttccta atgagaatac aaaaaagaga
     2221 ttgatgattt ttatacagga tttctttcga aaaacccgaa aagatcttca aaagacacta
     2281 taggatggct ctctacttta catgaatcta gaatatttct atttagcaat tccaataact
     2341 ctaactcatc tgctgcctct attacaaaaa aatgaccacg tttatctgca aattgtcctc
     2401 cagttagtaa ttttcct
//
LOCUS       DASVEV010000016         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1364462, whole genome shotgun sequence.
ACCESSION   DASVEV010000016 DASVEV010000000
VERSION     DASVEV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364462"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..443)
                     /locus_tag="VF222_00765"
     CDS             complement(15..443)
                     /locus_tag="VF222_00765"
                     /inference="COORDINATES: protein motif:HMM:NF021571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2299 family protein"
                     /protein_id="HEX7140988.1"
                     /translation="MADKIKTWLKDEDVHYNRIEDNSTDLHITLWFSKEIRLEAIVWN
                     EKLTISSNVIYFSEDIKNLLLVNENYRDELDLLLHQQIPNFVFLYSNDELTNLVGVRI
                     FKDIWADSLTKTSFFDTIVAVQHSVYIVKIKEREWAKLKV"
     gene            1092..1235
                     /locus_tag="VF222_00770"
     CDS             1092..1235
                     /locus_tag="VF222_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140989.1"
                     /translation="MLSLIIALFIKQIASNYTNLKAVLAWIANRISNKYDNIVEMSYA
                     TEY"
     gene            complement(1366..1803)
                     /locus_tag="VF222_00775"
     CDS             complement(1366..1803)
                     /locus_tag="VF222_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7140990.1"
                     /translation="MATEENMRLMKMLDDAWNSQEWDTFNKLHTNDVTVYWPGQSEPT
                     RGVHIHQKEAEMFFNAFPDNRIENDPYLVFFGHENWTCSIANFSGTHTGAMVGENGKT
                     IPPTNKKFYIELCTVAHWNNGQIMEEKIFYDLVGLMRQLGWIQ"
     gene            2038..2166
                     /locus_tag="VF222_00780"
     CDS             2038..2166
                     /locus_tag="VF222_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX7140991.1"
                     /translation="MAKDPVCGMNVDESKTQYISEINGQKVFFCREACQREFDKKS"
     gene            complement(2819..>3267)
                     /locus_tag="VF222_00785"
     CDS             complement(2819..>3267)
                     /locus_tag="VF222_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140992.1"
                     /translation="REIPRYTQRIAEYQEQTIQTVKDIASDFIEAEKEVIGSFQSQVD
                     RNNGNTYGLWDLYNPQRIAENHAVMVNNFTSYLLNTANLINNALASNMRVYNTALEQT
                     RDNLKAFAKTNTNYIKAVSVDSQNREVNTNSSSNNNNNSNERTTRL"
BASE COUNT         1174 a          450 c          414 g         1229 t
ORIGIN      
        1 ttacaaaaat aatgttatac ttttagttta gcccattccc tttcctttat ctttactatg
       61 tagacactat gttgaactgc aactattgta tcaaaaaatg atgttttagt taaagagtca
      121 gcccaaatat ctttaaaaat acgtacacca accaaatttg ttaattcatc attgctgtac
      181 aaaaatacaa aatttggaat ctgttgatgt aatagtaaat ctagttcatc acggtaattt
      241 tcattaacca gtaaaagatt tttgatatct tcggaaaaat atatcacgtt tgatgaaata
      301 gttaatttct cattccagac aattgcttct aaccttatct ctttactaaa ccataatgtt
      361 atatgtaaat cagttgaatt atcttctatt ctattataat gaacatcttc atcttttaac
      421 caagttttaa ttttatcagc catctttaca tcttccataa tttttatgta aattgattaa
      481 atatttagat gtatgctagc ttagccaggt aaaatatcac ttcagaaata cataacttga
      541 ttaattctat gcggttggtt agcgacatgt aaaatacata gataacaaaa tccaaagaaa
      601 atcccattga caaatattat gtcaaattta ataaaaaaaa tatataaaat aataaaacgt
      661 ttttatggag atataatttt gaatttatgt ctagagtgat atattcttat cattaacgcc
      721 aagattatag ttattaagaa ttattcttca ataatcatta atatctaatt gcgaaaaagt
      781 ataattttta gaccatgcaa tctattaaat tttattttaa aacttattac taatagtgaa
      841 attagtaacc ttgaaaagtc attcaaagtt agtattaaaa atattggaat ttacacattt
      901 tacaggcata gatattaaca acggattatc tcaatctatt tagttatggt aaactacgtt
      961 ttacaagatg aaagtaataa aataattgtt tttcagaaat ttagtttatt tcaaaaagat
     1021 aagtcaatta tattaaagta ttatgaatag aattataatt gtcatttcaa caatatagca
     1081 gattatcgtt attgttatca ctaataatag ccttgttcat taagcaaata gcttctaatt
     1141 atacaaatct aaaagcagta ttagcttgga tagccaatag aatatccaat aaatatgata
     1201 atattgtaga aatgagttat gcaacagaat actaacaaaa tggtattact aatagtattt
     1261 ctaaagatgc aatatgattg aaaaaaataa tagcattcta atcaattcat aattcaataa
     1321 aatgtaaatt atacttctac tattaatgaa tcatttttta tttacttatt gtatccaacc
     1381 taattgcctc attagtccta ccaaatcata aaatattttc tcttccatta tttgtccatt
     1441 attccaatgt gctactgtgc agagttctat ataaaatttt ttatttgttg gaggaatagt
     1501 ttttccattt tcaccaacca tagcccctgt atgtgtacca ctgaaattag caattgaaca
     1561 agtccaattc tcatgaccga aaaatacaag atatggatcg ttttcaatgc gattatcagg
     1621 gaatgcatta aaaaacattt cggcttcctt ttgatgtata tgtactcctc tagtaggttc
     1681 ggattgacct ggccaataca cagtaacatc attagtatga agtttattga aagtatccca
     1741 ttcttgtgaa ttccatgcat catccaacat tttcatcaaa cgcatgtttt cttcagtagc
     1801 cataattgtt ttacttaaag gctaaaatta aggatttctg gacaattaat ttacaaatct
     1861 gtgattaaat tgataaaaat cattttatcg aagcttattc ttcattttaa gaagaataca
     1921 ttcctcctat atctaatagt tagaaagatt tcataatatg agtatcaaac taaaattgct
     1981 ttacaaatga agtagtgcta tccttaaatt tcttaatgta caataataca tgcaaaaatg
     2041 gcaaaagatc cagtttgtgg aatgaatgtt gatgaatcaa aaacacaata tatttcagaa
     2101 ataaatgggc agaaagtttt tttctgtcgt gaagcatgtc aaagagaatt tgacaaaaaa
     2161 tcctaaaaaa tatggatatt aataaatatt gtaagtgtta taatatatgt tatccatgtc
     2221 tcactacata tttttctaat gcttcttgat ttcttatgag aaatgtttta actctaataa
     2281 tattatttgg tatattattt tcaataatca ctataactgt actacatatt ttgataaatg
     2341 cgggtatggt tcaagaaaaa ggaattacac caattgatcc aaaaaagctt aactaatcaa
     2401 acattacagg atattccaac tgaaggatac agatatgtca aagggtattg aaaataaaaa
     2461 caattaaaat tggtcatcat atatttttag attaacattc aacattagtt atctgtataa
     2521 cttctaactg agcaacttcc attttacctt gactctaata ataaggttat aaattatttt
     2581 ttgattgttc atatatataa tacaaaaaag agagtgaacg aattagattg attaattaat
     2641 aataaccaga attactaaaa tgttattgat ttgttattat tatttgttaa cgataacttc
     2701 tctgatattt tattaatact tattaggagc atataaaatg attatttgta aatttaaaaa
     2761 gaatatatcc tataatctaa aaaatacaaa agattatcgg actaggttta aaaatttatt
     2821 aaagtctagt tgttctttcg ttgctattgt tgttgttgtt gctgctgctg ttagtattta
     2881 cctctctatt ttggctatca actgatactg cttttatgta attggtgttt gtttttgcaa
     2941 aagcctttag attatcacgt gtttgttcta gtgctgtatt gtatactctc atatttgatg
     3001 ctagagcatt gtttattaga ttggcagtat ttaacaggta acttgtaaaa ttatttacca
     3061 ttaccgcatg attttctgcg attctttgtg gattatacaa atcccataac ccataggtat
     3121 ttccattatt tctatctact tgtgattgaa aagagcctat tacttctttt tctgcttcaa
     3181 taaagtcaga agcaatatct tttacagttt gaatagtttg ttcttgatat tcagcaatcc
     3241 tttgtgtgta tcgtggaatc tctcgtg
//
LOCUS       DASVEV010000017        12184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_781912, whole genome shotgun sequence.
ACCESSION   DASVEV010000017 DASVEV010000000
VERSION     DASVEV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781912"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VF222_00790"
     CDS             <1..1594
                     /locus_tag="VF222_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7140993.1"
                     /translation="KGVLLHGPPGTGKTLLAKAVANETNANFYSIGGPEIMSKFYGES
                     EEKLRDIFLQAQEKAPSIIFIDEIDSIAPKRDEVSGDVEKRIVSQLLTLMDGLKARGK
                     VVVIAATNRPNAIDPALRRPGRFDREIEIGIPDEGGRYDILQIHTRGMPLEQDIDLKF
                     YSKVTHGFVGADLEMVAKEAAMRSLRRIIPQINLKESKIPIDILNKIKITSQDFDDAL
                     KEVHPSAMREVLVQKPNIEWKDIGGLDNVKEELKEAIEWPLKHADLFSKAAIKPPKGM
                     LLYGPPGTGKTLLAKAVASTSESNFISIKGPELLSKWVGESEKGVREIFRKARQAAPC
                     VIFFDEIDAIAPRRSGSDGGDSHVTERLVSQMLTEIDGLEDLKGVVIIGATNRPDIID
                     EALLRPGRFDRLLEIPIPDNNTRKQILQIHLKKKPLGSDVNIDKLVNLTDSFTGAEIE
                     ALVNAAAIAAVKEHVITREQKEQNKNQRTDTNIKDTFKIQEKKENKLKEELEISMKHF
                     ESSFSKIAKKNKPSLPRISSIA"
     gene            complement(1834..2280)
                     /locus_tag="VF222_00795"
     CDS             complement(1834..2280)
                     /locus_tag="VF222_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7140994.1"
                     /translation="MIQFINKFIINEDIEKVWDFYTDIRHLEIITPKKLNLKIINTSN
                     QKIILGQETILSAKIIIILRTWHSKITFFQQYEYIDEMLEGPFMKWKHTHKFERINKN
                     KTEIIDEVEFSLPYGAFGKIVSIYVTKILRQLFKYREDETIKYLSR"
     gene            2406..3137
                     /locus_tag="VF222_00800"
     CDS             2406..3137
                     /locus_tag="VF222_00800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX7140995.1"
                     /translation="MSNKNILVIQNIGCEHLGSLAKLFELDGFNVTILNSQSNLIPID
                     LLTYSGIIILGGPMSVYDNYVYLKNEQKLIKKAVDMNIPTLGICLGSQLIAEAMGGKV
                     YPGNLKEIGWHDIEITENGSRDIFNGITTLKNTVFQWHGDTFELPESAIILAKSETYV
                     QAFRIKTAIGIQFHIEVDESMIDEWIRVYSKEISDLNLDKSEIISQQKVQVLDLLMLC
                     KIIYNNFKKMIYYYYYDDKYNHQKC"
     gene            complement(3259..4344)
                     /locus_tag="VF222_00805"
     CDS             complement(3259..4344)
                     /locus_tag="VF222_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7140996.1"
                     /translation="MLILLASGTFFGIANAQNLNETSSNNNNKSLSLNDLFELVENSV
                     VQVTKTMPPANPFGPDKENTTSLGSGFIYNDKGYIVTNNHVVENAKVVDITFINGDRY
                     TANITGTDAFSDLALIKINETTTDLPKPLVIGNSSELRVGDQVVAIGNPFGLDSSMTT
                     GIVSQIGRLLSIDERGFSIPNAIQTDALINPGNSGGPLLNMKGEVIGVNTAGIFPGGI
                     GLAVPSNTILKIIPVLMEEKNYTHPWLGVTGNTLTADIAKREKVDRTLKGVIIDNIVK
                     SSPADLAGLNGSNIDQYGEKRGGDLITAVDGKPIIKMEDLISYLELNKAVNDNATLSV
                     YRDGEVIDKEVTLKSRPAAPVTNQTQN"
     gene            4646..5782
                     /locus_tag="VF222_00810"
     CDS             4646..5782
                     /locus_tag="VF222_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140997.1"
                     /translation="MYSKNLKFLYYSDLEVSLFILIVSTLGSFMQISGASWDITSHLL
                     NQPDSFFTPSHAMLYTGIGLIAISAGVGSYLLNRKKIKQYNSISRSFKLLIIGSFLSL
                     IAGPLDYLWHQSFGLDGLLSPTHMTLATGMLINSVAVFIGLYGVMNNISHWKNLPKIF
                     LIPCLSSLWLTSIWYVFILVLPFSESDFFNFNLDTYSATLIASIFLPLINSIMFLCSS
                     KVFNNTFGYTSIIIALLVLVNTFSTILPTGNILSSIIPWYFVSTLGASLLADIILNSN
                     FRINSKQFKNNKLIASSIIGSIFYFFNYPMIVWTYGTFLNVNLGSLNAVLPTFLNTIV
                     IVTCITVPVGTVMGLIGYLIFSKIENSLNILTKGRNSTVLKSDH"
     gene            complement(6074..7021)
                     /locus_tag="VF222_00815"
     CDS             complement(6074..7021)
                     /locus_tag="VF222_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7140998.1"
                     /translation="MSINLRNRNIVYILFFVLGGVLLSIFNSNNDSYYKVYAQYENIA
                     INTTKLTDSLYMLQGSGGNILVSVGQNGVFMVDDQFAPLTVKIKDAISKITDKPIQFV
                     INTHWHPDHTGGNENLGEAGTIIVSHDNVKKRLSTEQFSDFFKRSVPPLSEKGLPIIT
                     FSDNMTIYQNDDQINIIHMDNGHTDGDSIVYFTKNNVIHVGDDFSDKAYPFIDISSGG
                     TVEGLISSLKTISSIINDETKVVSGHTGISNKTKVNEYANMLTDVRNQISQMINEGKS
                     LESIITLQPTSKYDKIYYDNTNIKPEDFITHVYQSLTQK"
     gene            7515..7718
                     /locus_tag="VF222_00820"
     CDS             7515..7718
                     /locus_tag="VF222_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7140999.1"
                     /translation="MKNDYRIDHITIPIGLKDVLNKAGFTIESILNNDPSKIAQDLGI
                     DEYVGKIIFDETKRKFENLRNAF"
     gene            complement(8194..8382)
                     /locus_tag="VF222_00825"
     CDS             complement(8194..8382)
                     /locus_tag="VF222_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase family protein"
                     /protein_id="HEX7141000.1"
                     /translation="MPVIQITMSEGRTLEQKRELVRVLTKETARILNSKEENMRVLIY
                     EVSKENWGDAGILGIDMK"
     gene            8516..9382
                     /locus_tag="VF222_00830"
     CDS             8516..9382
                     /locus_tag="VF222_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141001.1"
                     /translation="MKKNNVLKFTSIFSIAIFVISFSLVDQLSRVYAHSFTSDDASYF
                     LGLDKRTKVELELAEKNYPLNITLSMDHADNAARLVYDIYYADDDIVDDNDFIKRYNK
                     EVSSSNSTTYALVVADLLDEVLRGYADALVIDTDLTNMSNLVLIDELKNLAKESNSTT
                     VPSNSSDKEEGSESEYSKLLSTNNSIFNYADYETAKALLVEIKDIFENHLKSPSNNAT
                     SQSVNVISKLEKDLEKLSNITNNNDGSPAEVMELVHLQIHPSLQKGFGLQTKMNMNGQ
                     MNMNGQMNMNGG"
     gene            9578..10534
                     /locus_tag="VF222_00835"
     CDS             9578..10534
                     /locus_tag="VF222_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7141002.1"
                     /translation="MIRKSWIVFLFAVILSFESILIEYLTLLLGISPLAISAFSIAIS
                     GILLLLTLNFVSNRQTPLFYSFKDFVPASIFLSVGIFTWYDAVSKVGASKESLLAGPI
                     EIIAVLFLARIFLHEKLNKKQVSGVGIALVGFIIVILSDHNISNNNGIINVTIIEVEL
                     LNFSISTGDIEAIISAISFALAVFFLAKLSRKYSPLEVSGMCLLLSGFILIIVMLIFT
                     PEVSVNLFMSYWYIFIIFSLLPLIGTICYIEGIKRIGASLTSTIASSRILLTLIIQIL
                     LTQIGIRNTLPDNVFLALIGGTLGITGIIIIHMHFYFSKHTN"
     gene            11261..>12184
                     /locus_tag="VF222_00840"
     CDS             11261..>12184
                     /locus_tag="VF222_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141003.1"
                     /translation="MNNQNENTPTHTKNEEENEEAEVMLHCSLCGKKLFANESSDIII
                     EEIAGAQYHFDNPQCVIMFKRLNHAYGKNLKNFMGTTQFISDPFWDKTIPTEQEINEI
                     NQEVSKPDIRVINNPEEALKRADQLIKSAKNEILLLFSSANALHRRLETADSLDTIRE
                     VIQTHKEIKIRVLTPFDEKVSDLVKYLKESLNIDVKFLAEPLQLKVTIVVVDRSYSMA
                     VELVNDDVYNSIDAMGLSTYSNSKSTVLYYVSIFESLWKQADIYKKAEELYEQLKYKN
                     ETQKQFLNIAAHELRNPIQPILGLAEVMLSNK"
BASE COUNT         4276 a         1712 c         1798 g         4398 t
ORIGIN      
        1 aaagggtgta ctattacatg gacctccagg tacgggtaag accttgcttg caaaagcagt
       61 tgctaatgag acaaacgcta atttctattc tatcggcgga ccagaaatta tgagcaaatt
      121 ttatggagaa agcgaagaaa agttaagaga tatattccta caggcgcaag aaaaggctcc
      181 cagtattata tttatagatg aaattgattc tatagcaccc aaaagagatg aagtttccgg
      241 tgatgttgag aaaagaatcg tatctcagct tttgacttta atggacggac tcaaagcaag
      301 aggcaaagtt gttgtaattg ccgctacgaa tagaccaaat gcaattgatc cggcattgag
      361 acgacctggt agatttgata gagaaataga aatcggtatc ccagatgaag ggggacgtta
      421 tgatattctt caaattcata caagaggaat gccattggaa caagatatag acttaaaatt
      481 ttattccaaa gttacccatg gatttgtagg tgcggatttg gaaatggtag cgaaagaagc
      541 tgcaatgaga tctttacgta gaattatacc tcaaataaat ttaaaagaat caaaaattcc
      601 gatagatata ttaaataaaa taaaaataac tagccaagat tttgatgatg ctttaaaaga
      661 ggtccatcca tctgcaatga gagaagtttt agtgcaaaag ccaaatatag aatggaaaga
      721 tatcggaggt ctagataatg ttaaagaaga attaaaagaa gcaatagaat ggcctcttaa
      781 acatgcagat ctttttagca aagcagcaat aaagcctcct aagggaatgc tattatatgg
      841 acctccagga acaggaaaaa cactattagc taaagccgtt gcatcaacat ctgaatcaaa
      901 ttttattagt ataaaaggtc ctgagctatt atcaaaatgg gttggtgaat ctgaaaaagg
      961 agttagagaa atctttagaa aagctagaca ggcagcacct tgcgtaatat tttttgatga
     1021 aatagacgct attgctccaa gaagatcagg atctgatgga ggagattcac atgtaactga
     1081 aagattggtt agccaaatgt taactgagat agatggatta gaagatctca aaggagtagt
     1141 gattatcggt gcaactaata gacctgatat tattgatgaa gctttgctaa gacctggaag
     1201 atttgatcgc cttttagaaa taccaatacc tgataataat actcgaaaac aaattttgca
     1261 aatacaccta aaaaagaaac cattaggttc tgatgtcaat atagataaat tagtaaattt
     1321 aacagacagt ttcactggtg cagagattga agctttagtt aatgctgctg ctattgcagc
     1381 agtaaaagaa catgttataa ctagagaaca aaaagaacaa aataagaatc aaagaacaga
     1441 tactaatata aaagatacat ttaaaattca agaaaaaaaa gagaacaaat tgaaagaaga
     1501 actagaaata tcaatgaaac atttcgaaag ctcatttagt aaaattgcaa agaaaaataa
     1561 gccctctcta cctagaatta gcagcatagc gtaatcctat aaatcaatca ctttttgtat
     1621 ctgtactgct ctatagtctt ccaattaata attgaccacc agcatcatct agtctaaatt
     1681 caagcaaaca aaaaagtatt tctagaaaga aaatctattt tttatttttt tgggtatatt
     1741 tattatttat aatttcaaaa taaaacttat aatataatta tatccataga ttttttatta
     1801 tagaaactta aaattaatta ataatttaaa agtttatctg cttaaatatt taatggtttc
     1861 atcttctcta tacttaaata attgtcttaa tattttggta acatatattg aaacaatttt
     1921 cccaaaagct ccatatggta aactaaattc tacttcatca ataatttctg ttttattctt
     1981 gttgattctt tcaaatttgt gtgtatgttt ccatttcata aaaggtcctt ctaacatttc
     2041 atctatgtat tcatattgct gaaaaaaagt tatttttgaa tgccatgttc tcagtataat
     2101 tattatttta gctgaaagaa tggtttcttg gccaaggata attttttgat ttgaagtatt
     2161 aattattttc aaatttaact tttttggcgt gataatttct aaatgtctaa tatcagtata
     2221 aaaatcccac actttttcaa tatcctcgtt tattataaat ttatttatga attgaatcaa
     2281 gcaagaaaat attagatatg atctaattca aatgttgtca tagattttca acaaataaat
     2341 ctatgaaagt taattttttt aatataatag ctttatattg aattgtcgaa actttagttt
     2401 tatctttgtc taataagaat attttggtta ttcaaaatat aggatgtgaa catttaggta
     2461 gtcttgctaa gctgtttgaa ttagatggat ttaatgtaac aatattaaac tctcagagta
     2521 atttgattcc tatagatctt ctcacgtact caggcattat tatcttgggg ggacctatgt
     2581 cggtttatga taattatgtc tacttaaaaa atgaacaaaa attaattaaa aaagcagtag
     2641 atatgaatat accaacattg gggatatgtc ttggttccca attgatcgcc gaagcaatgg
     2701 gtggaaaggt ttacccagga aacttaaaag aaataggttg gcatgatatt gaaattactg
     2761 aaaatggaag tagagatata ttcaatggaa ttacaacttt aaaaaataca gtttttcaat
     2821 ggcatggaga tacgtttgag cttcccgagt ctgcaatcat tttagctaaa tccgaaacct
     2881 atgtacaagc ttttcgtata aagactgcaa taggaatcca atttcatatt gaagtagatg
     2941 aatctatgat cgatgaatgg ataagagttt actcaaaaga aatatcagat ttgaatttag
     3001 acaagtctga aatcatatct caacaaaaag tgcaagtttt agatcttcta atgttatgta
     3061 agataatata taataatttt aagaaaatga tttattatta ttattatgat gacaaatata
     3121 atcatcaaaa atgttgaatc aataattata tattttcatt actaaataaa tgttagccta
     3181 ggttaacttt tgtgaaaagt caattataat gagttttttt gatttataaa aaaagagtga
     3241 aaggaaagga aataaaatct agttctgtgt ttgattcgtt accggagctg ctggtctact
     3301 tttcaaagta acttctttat ctataacctc tccatctcta tatactgata atgttgcatt
     3361 atcatttaca gccttgttca attctaaata tgatatcaaa tcttccatct tgattattgg
     3421 tttaccatct actgctgtaa taaggtctcc tcctctcttt tcaccatatt gatcgatatt
     3481 actgccattc aatcccgcta aatctgcagg gctactttta actatattgt ctatgataac
     3541 accttttaga gtcctatcta ctttttccct cttagcaatg tctgcagtta gtgtatttcc
     3601 agttacacca agccatggat gagtataatt tttctcttcc atcaaaacag gaattatttt
     3661 taagatagta ttagatggaa cagccaaacc tattccacca ggaaaaatac ctgctgtatt
     3721 aactcctata acttcgcctt tcatatttaa tagtggaccg cccgaattgc caggattgat
     3781 taatgcatcg gtttgaatag cattgggtat tgaaaatcct ctttcgtcta tagataagag
     3841 cctacctatc tgactaacaa ttcctgttgt cattgaacta tctaacccaa aaggattgcc
     3901 tatagctact acttgatcac caaccctaag ctcagacgag tttccaatta ctaatggttt
     3961 aggtaaatcc gttgtagttt cattaatctt tataagtgct aaatcactga aagcatctgt
     4021 tccagttata ttagctgtat atctatctcc attaataaaa gttatatcaa ccacttttgc
     4081 attttcgaca acatgattat tagtaacaat gtatccttta tcattataaa taaatccaga
     4141 acctaatgag gtagtattct ccttatcggg cccgaatggg ttagcaggag gcatcgtttt
     4201 tgttacttga actactgaat tttctaccaa ttcaaagaga tcatttaagg agagactctt
     4261 gttgttgttg ttgctagatg tttcattcaa attttgggca ttggctattc caaaaaacgt
     4321 accactggct aacaatatta gcattaaaac aatagtacca ctattcctat tgattatgat
     4381 ttttgacatc tgaattatta aagtattttt gttatttcta tatgtttctt ttaagataag
     4441 catgatacta caaagttatg gtaatttttt atttttgttt tgattatctg agcagattat
     4501 tccctctatt agtttatata tatctttgtt atttcctagt aattgtagca ataagcatta
     4561 cttgtctcat atttgctttt tgctgttaat ttactattat tagttatact tattagttat
     4621 attggcaaag aatgtttgtg aatatatgta tagtaagaat ttaaaattct tatactattc
     4681 agatttagaa gtatcattat ttattttaat tgtttccacg ttagggtctt ttatgcaaat
     4741 ctctggggca agttgggata taacttctca tctcttaaat caaccagatt cgttttttac
     4801 cccatctcat gctatgcttt atactggaat agggttaata gcgatctctg caggagtggg
     4861 ttcttatcta ttaaatagaa agaaaattaa acaatacaac tctatttctc gttcttttaa
     4921 attattgata ataggttcat ttttgtcatt gattgcaggc ccgttggatt atttatggca
     4981 tcaaagtttt ggactcgatg gtctgctaag tccaactcat atgaccttgg ctacaggaat
     5041 gctaattaac tctgtcgcag ttttcatagg gttatatggt gtaatgaata atatttcaca
     5101 ttggaaaaat ttacctaaaa tttttcttat tccttgtctc tcttcgttat ggcttacatc
     5161 catatggtat gtctttatac tagttttacc attttccgaa tctgattttt ttaattttaa
     5221 tttagataca tatagtgcaa ctttgatagc aagtattttt cttcctctca ttaattctat
     5281 tatgtttctg tgctcatcaa aagtattcaa taatactttt ggctatacat ccataataat
     5341 tgctttatta gtattggtaa atactttttc aactattctt ccgactggaa atatactctc
     5401 ttctattatt ccttggtatt ttgttagtac tttaggggcc tctcttcttg cagatataat
     5461 attaaactca aactttagaa ttaatagtaa acaatttaaa aataataaat tgatagcaag
     5521 ttctataatt ggttcaattt tctatttttt taattatcct atgatcgtat ggacatacgg
     5581 aacctttcta aatgttaatt taggatcttt aaatgccgta ttacctacat ttctaaatac
     5641 tattgtaatt gtgacttgta ttactgtacc agttggtaca gttatgggat taattggata
     5701 ccttatattt tcaaagatag aaaattcttt aaatattttg actaaaggaa gaaattctac
     5761 tgttttgaaa tccgatcact agatcaagta gtaatgagta taaatctgtt ttttaaacaa
     5821 cattaaatag ttataattac aacatcatca tatacctttt tttaataatt tgtaggttat
     5881 tttaaaaaat atttttattt tatttattat tttcaagaaa aatgttgctt agtgataaaa
     5941 aaattgtcaa taaattacga atatgttagt tgcgatttta taatagtgga tataggcact
     6001 ccctttttta ttcaatatat tttgggtcca gtttatcata gacatttaat agtaaaatga
     6061 aataggaatt gtcttatttt tgtgtaagac tttgatatac atgtgttata aaatcttctg
     6121 gttttatgtt agtattgtca taatatattt tgtcatattt tgaggttggt tgtaaagtaa
     6181 taatactttc taaagattta ccttcattta tcatttgact tatttggtta cgtacatcag
     6241 ttaacatgtt tgcatattca ttaactttgg tcttgttaga tattccagta tgtccagaaa
     6301 ctaccttagt ctcatcatta attatggatg atattgtttt taacgatgat attaatcctt
     6361 ctacggtgcc accacttgat atatctatga atggatatgc tttgtcgcta aagtcatcgc
     6421 caacatgaat tacgttattc tttgtaaagt aaactataga atcaccatcc gtgtgaccat
     6481 tatccatgtg aataatattt atttggtcat cattttggta gatggtcatg ttatcggaga
     6541 aagtaataat tggtaaacct ttttctgaca atggtggaac cgatcgtttg aagaaatcag
     6601 agaattgttc tgtactgagt cgttttttta cattatcatg tgaaactata attgtccctg
     6661 cttctcccag attctcattg cctcctgtat gatcaggatg ccaatgtgta ttaattacaa
     6721 actgtatcgg tttatctgtt attttggaaa ttgcatcttt aatttttaca gtaaggggtg
     6781 caaattgatc gtctaccata aagactccat tttgtccaac agaaactaga atattgcctc
     6841 ctgatccttg aagcatatat agactatcag tcaattttgt tgtattaatt gctatatttt
     6901 catattgagc atagacctta taataagaat cattattgct attgaaaatc gataataaca
     6961 ctccacctag aacaaaaaat aatatatata caatatttct atttctgaga tttattgaca
     7021 tcagttctaa acttttagat ttttgtagat tcattttgtt attatgtatt ctaataaata
     7081 aaagtatttc aatatttaaa atgcggtcat tagttgggca aaattaataa taatatcaaa
     7141 ttagtttagt attgtttatc tctatcttta atcttctaga aatactcatc agagagcaat
     7201 aacctatgat atcatctgaa agatagatat atagtataca aagatcgtaa catggaatat
     7261 tttatttcct tcctaccccc tacccccact gataatgtaa tatgaaaaag tgtgaacgaa
     7321 acgatattag attagtgtta ataatttaat tacgatatca aatatattgt ttgtatatta
     7381 ccataaaata agatctaata ttaataacgt ctgccataca ccgttaaaat cttaaattag
     7441 atatactata tagttccaag attaatatat aggatagttt tttcttcctc gagttgctaa
     7501 tatacagtac agatatgaag aatgactaca gaattgacca tattacaatt cctattggac
     7561 taaaagatgt attgaataaa gcaggattta caatcgaatc tatacttaac aatgatccgt
     7621 ccaagatagc tcaagattta ggaattgacg aatatgtggg aaaaataata tttgatgaga
     7681 caaaaagaaa atttgaaaat cttagaaacg ctttttgaga agtatttgat tttttgaact
     7741 attataataa ttaaattttc aaaatttaaa gtaatatcag atattctatt tatcaatcca
     7801 ttaattaatt aatgtaattg agtcaattcg cattacacaa ataactattc tgagagtttt
     7861 tcaaactgtt gtgtatgtaa atataactaa attcgaagaa ttctaataaa atagtgccta
     7921 caatttttta ctagggaagg ttgtgattta gaatgaaaaa ctatatacac aaaaaatatt
     7981 gttgacctaa gattttcaga aaaaaacaaa agtattttaa aaaattgccg aagaatacag
     8041 tctagaacgt tttaattttg ttttatttat gtctacagat aaataaataa attcagtaat
     8101 ataaacagaa gagaaaaata tcatcaccaa cttgattcat caccaacttg attcattatt
     8161 attcaactgt tacttcattt tctcaattct cgtttacttc atgtctattc ccaatattcc
     8221 agcatctccc caattttctt tagaaacttc ataaattaag actctcatgt tttcttcttt
     8281 tgaatttaaa atgcgagctg tttccttggt taagaccctt actaattccc tcttttgctc
     8341 aagagttcta ccttcgctca ttgttatttg tattacaggc atgttactaa tgattaaaaa
     8401 ccttatcaaa tcaattttat gaatgttatt atatatctta atcagaataa atcaaaatta
     8461 agatagaaaa tactttcata acagttttat tataacatca acttttatta ttgaattgaa
     8521 aaaaaataat gttctaaagt ttacttccat ttttagtata gccatttttg ttatttcatt
     8581 ttctttggtt gatcagctat cgcgtgttta tgctcatagt tttacttctg atgatgcatc
     8641 ttattttcta ggtttagata aacgtacaaa agtagaactg gaactcgcag agaaaaacta
     8701 tcctctcaac attactttat ctatggatca tgccgataat gctgcaagat tagtatatga
     8761 tatttattat gctgacgatg acattgtgga tgataatgat tttattaaaa gatataacaa
     8821 ggaagtttcc tcttccaatt caacaaccta tgcactagtt gttgctgatc ttttagatga
     8881 agtattaaga ggatatgctg atgcattagt aatagataca gatcttacaa atatgtctaa
     8941 tttggtcctc atagatgaat tgaaaaatct agcaaaagaa tctaattcta ccacggtccc
     9001 ttcaaatagt tcggataagg aggagggatc tgagtcagaa tactctaaac tcctctcaac
     9061 aaataattcc atatttaatt atgctgacta tgaaacagca aaggcattat tagttgaaat
     9121 caaagacata tttgaaaacc atcttaaatc accatccaat aatgctacga gccaatctgt
     9181 aaatgttatt tcaaaactag agaaggatct tgagaaatta tctaatataa ctaataataa
     9241 tgatggttca ccagcagaag tgatggagtt ggttcattta caaatacatc catctttgca
     9301 gaaaggattc ggattacaaa cgaaaatgaa catgaatgga caaatgaaca tgaatggaca
     9361 aatgaacatg aatggaggat aaaaattaaa taaatttaaa tgatattaat tattatatta
     9421 aaatttatat atatataatt ttttgtttcc ttagaaagta attataatca attagctaat
     9481 tatctaaatc aaaaatgcaa aaaatttttt cttgagcaac tatttgatat gaaggaagat
     9541 atttatcttt atacaaattt taccatatta attactattg ataaggaaat catggatcgt
     9601 ttttttattt gccgttattt tatcatttga atcgattcta attgagtacc tcacattatt
     9661 attaggaatt tctccattag ctatatctgc atttagtatt gctatatccg gtattctgtt
     9721 attattaact ttaaattttg ttagtaacag acaaactcct ctattttatt cctttaaaga
     9781 ttttgttcct gcttctattt tcctttctgt tggtatattt acatggtacg atgcagtaag
     9841 taaagttggc gcatcaaaag aaagtctatt ggcaggacca atagaaatta tagcagttct
     9901 attcttggca cgaatttttc ttcatgaaaa attaaataaa aaacaagtgt caggggttgg
     9961 tatagcactt gtaggattta taattgtgat attaagtgat cataacatta gcaataataa
    10021 tggaattata aatgttacca taattgaagt cgaattatta aatttttcta tttcaacagg
    10081 tgatatcgag gcaattattt ctgcaatatc ttttgcatta gcagtatttt ttttagctaa
    10141 attatcaagg aaatattctc cattagaagt ttcaggaatg tgtttgctgt tatctggatt
    10201 tattttaatt attgtaatgc ttatctttac tcctgaagtg tctgtcaatt tgtttatgtc
    10261 ttattggtat atattcataa tattttctct gttaccatta attggtacta tatgttatat
    10321 cgaaggaata aaaagaatag gagcttcctt aacatctaca atagcatctt ctagaattct
    10381 gttaacatta attattcaaa tattattaac tcagattgga atcagaaata ctctacctga
    10441 taatgtattt ttagcattaa ttgggggaac actaggtatc actggaataa ttatcataca
    10501 tatgcatttt tatttttcaa aacataccaa ttaatttgta tcgtgagtta tttacaattt
    10561 aaatagttta agattctcat ccaacaatat tttatattat tttaaacata agtttgcatg
    10621 gatttgttgt tattgttgtt tcagataaag agacaatctt agtagttgta cagacttaaa
    10681 tacccttcaa caaagtaaac aaatagaaag ttttataaga tgaacttttg agtaaaatta
    10741 aaatataata tacaattcat catttgtatt ataactactt tatgacaaat attactatac
    10801 tttatatttt aaatttgtag taagtagata ttaaattcac agaagaaata tttataataa
    10861 tgttttaata aaattaatct atactcttta actttttaag tatacaaata attatagaaa
    10921 aaattttagc taaaaattat gtaaagataa ccatatccct tatatccttt aaaaaacaca
    10981 ttataatata ctgaaatctt gtattttatg tggtaaggtt ataaaaaaag atacaatctt
    11041 tacttatagt aagatgataa ataatttaag ttatgaattt acttttgaca aagaagactg
    11101 ttttcttttt ttcaagagac tggctagtgt ttatggcgaa ggttttgaac tagatgctct
    11161 gtgttctgta gagaaatgtc tattagttta aataattaat ctacccactt ttgcgaatac
    11221 tttaaaacag agaaaatatt acaagtacat atctcgaata ttgaataacc aaaatgaaaa
    11281 tactcctaca catacaaaaa acgaagaaga aaacgaagaa gcagaagtta tgttgcattg
    11341 ctctttatgt gggaaaaaat tatttgcgaa tgaatccagt gacattataa ttgaagaaat
    11401 cgcaggagca caatatcatt ttgataatcc tcaatgtgtt attatgttta agcgattaaa
    11461 tcacgcctat ggtaaaaacc tcaaaaactt tatgggtacc actcaattta tttctgatcc
    11521 attttgggat aaaacaattc ctacagagca agaaattaat gaaattaatc aagaagtaag
    11581 caagcctgac atcagggtaa ttaataaccc tgaagaggct ttaaaaagag ctgaccaatt
    11641 aattaagagt gcaaaaaatg aaatattgtt attattttcg tctgcaaatg ctcttcatag
    11701 acgacttgaa acggcagaca gtttggatac tattagagaa gtaattcaaa ctcataagga
    11761 gataaaaatc agagttctta caccgtttga tgaaaaagta agtgatttag taaaatattt
    11821 gaaagaatcc ttgaatatag atgtaaagtt tttagcagaa ccgcttcagc taaaagtaac
    11881 aattgttgta gtagatagga gttattctat ggcggtggaa ttagtaaatg atgatgttta
    11941 taactctata gatgctatgg gattgtccac ttattcaaat agcaaatcga ctgtattata
    12001 ttatgtttct atttttgaga gtttatggaa acaagcagat atctataaaa aagcagaaga
    12061 actttatgaa caacttaaat ataaaaatga aacacaaaag cagtttctca atatagctgc
    12121 tcatgaatta cgtaatccga ttcaaccaat tttaggttta gccgaagtaa tgctttccaa
    12181 taaa
//
LOCUS       DASVEV010000018         9415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1170281, whole genome shotgun sequence.
ACCESSION   DASVEV010000018 DASVEV010000000
VERSION     DASVEV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170281"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1174)
                     /locus_tag="VF222_00845"
     CDS             complement(53..1174)
                     /locus_tag="VF222_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141004.1"
                     /translation="MSNLQNENTKLHTKNSKEFVLQKISKDNRKSLTARVKPEDLIVF
                     NQRLKLFGFNSINELVHEFIAGRFPQITEDKQIDNLIGNTQSNGQKTILEVGNAGDFY
                     KNVDLDDMYNYYQNIRGLHPKTCKDLISYFRRFKDQFFTPQKGEELRMLSPRIRSKIM
                     DSFRKFGQYYLYKYNDDSCIDLVFKIIRRYNLNAGNNDHSKLYIVDDNYLAEKLRTVL
                     QLQGEIGLIVKFAMFSGLREDELVYIYRKEICSNLGGCSCEKLHVFKKPNGTSIILIQ
                     WHRGHKKCYFAIVPTTIWKLFRNLSFFDYNPHIKSAHSYLKAKDKDLHFMWLRKAHYN
                     IMCRSMKPFEANILAGRAKTVDAKHYAMYELDSMSKSYN"
     gene            complement(1458..1532)
                     /locus_tag="VF222_00850"
     tRNA            complement(1458..1532)
                     /locus_tag="VF222_00850"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1495..1497),aa:Ala,seq:cgc)
     gene            1771..3237
                     /locus_tag="VF222_00855"
     CDS             1771..3237
                     /locus_tag="VF222_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7141005.1"
                     /translation="MKLLTTGLMMGMLVAGLLLSPFVSGNPIINKVFAQEEEGPVKQV
                     LLVANENQLQIAPDNALHPGGIEYAAMTFNGTIPGPVIAGDQGDVLNITIRNDGKTIH
                     SLDFHAGLGPSNVLSGNIEPGQSKHVTLHAENPGAWMYHCGADGLNGVWEHIANGMYG
                     AVVVHPADEQPAKEFYLSFGENFNTADKGLFVGTNGTTGTFDLTKFATDQPDLVLTNG
                     MAHKYAPGIGEKVKIVLNENPEIFKVAPGELTRWYILNPGPNGGVSFHFISGMITTRD
                     GSTPDGKDYGTQDVMDETWWVPPGSASVFEAVFPETGAYVGVDHNMNHVVKGAAFIVM
                     GDTNSTAKDQPPGTAVAPKGSESAVADWSKPKPYETIIAEASAETGDITANATLAYAQ
                     ETISMPAGAANPSNKEFYVPAETSVPTGTTVTWSNDDTTIHTVVQGSPQGGAGETPAF
                     DSGIIAPGATFENTFDTAGDFDYYCSLHPFMVGKVTVT"
     gene            3351..4961
                     /locus_tag="VF222_00860"
     CDS             3351..4961
                     /locus_tag="VF222_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7141006.1"
                     /translation="MQNSCDLLIKNGIVVIPKVGVLNTNIMIENGKIKELSRSLNNIS
                     YSRSIDATGKYILPGLIDPHVHYGVYTPIEEAARTESKSAAIGGVTTMIRMLRLYSDY
                     RSNIKKQIEASLKNHIIDFSIHSSLLIDQHLQDISYLIQETGINSFKVYMNLGSKLNQ
                     IHMDLNPGEKDIRSGKVEITDGFLNEIVKTTAFFNSMLLVHAEDAEICYKQIKKELID
                     NNKSNNNDLLLLESKKNTRQATGNISLNGKSSFKENIDETTEEEEQKKDSNTDKKKFK
                     APKDQKLEENLLELWSRCRPIHSEVKSIQKIAELGRKYNSDIYFVHIGSSAAIDAIIQ
                     EKVKGQCNLYIETCPHYLTHTYDYNDLKGKVVPPLRSKHDLQSVWYALRNGIIDTIGT
                     DHVANRLSLKLDRNGDLLESLSGFPGIATMLPVLLSEGVNKRRINLQRVTEVTSYNTS
                     RIFGLYPQKGSIQKGSDADLVIVDLDLKKKVTPDLLQSYSDYTIYDGWELCGWPITTI
                     LRGKVIMENNLVDENSYGYGKFIQRFKN"
     gene            complement(4996..5766)
                     /gene="rpiA"
                     /locus_tag="VF222_00865"
     CDS             complement(4996..5766)
                     /gene="rpiA"
                     /locus_tag="VF222_00865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEX7141007.1"
                     /translation="MFENIIKNHFLKLFDKKNFKKENIIIGLGSGSTVAEFVKKISTV
                     SNKEKFNFIPTSIQIKIIAEKAGLKFTDETKINILDLVVDGADQIDHNLDMIKGGGGA
                     LFKEKIIMYSAKKKIILADSKKFVPKFTIPIPIEVHPFARSTVTNRLESMEIIGGGGK
                     DGITNGSQMNNRRDKKSYLNLSAKPVIRTIQKGYPFITENGNIILDTEFSSILDVEKA
                     EKEIKNIPGVIEVGLFSRKNNTHYYSVNSEGKYSVKSS"
     gene            6793..7206
                     /locus_tag="VF222_00870"
     CDS             6793..7206
                     /locus_tag="VF222_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7141008.1"
                     /translation="MFGRIGSVILLVDDLERSAQFYKDTIGLKLKVKTTDWIEFFEKG
                     TTLALHPRKRRKDVDDDRDGHPNVLVGFMVADLDQVVKDLRAKDVRFFKEPKTEPFGK
                     HAIILDPDDHLISLAEIESTRSNEEFDLIGFLGAE"
     gene            complement(7264..8736)
                     /locus_tag="VF222_00875"
     CDS             complement(7264..8736)
                     /locus_tag="VF222_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141009.1"
                     /translation="MVDEDSFLLIITGISIIGGIIFGLYYTRIFSSFIFYLKKNSSKS
                     NNSSATKEENKVKELEDVLKTKPDLIKKNSQLMEDELKIYQFEKDLATHAIENILLAS
                     KNKSIDSFEKDRLLLKYKEQLKKLNERMEKIQSEIDVTKLIILRDDLAFLLDNKISDI
                     DEKIKEINFKIGTNYKVKEMANQDRINKTNDNNLKNIDNTFIDIEETVDANLSLRELT
                     SYKNNPSHKRKEMMIETEREKIDDLKDQVLVALNRLDKIGDIKMEIDHQPDTKKVIQD
                     NIFTFTDKDLRNNFQEKLEVNDKEHLSTQNDKKYVNNLTEKNLNIPKIFSFRDVKSEN
                     EIVTTNNGTFDPLRKIAVEGGLNNEIDVNLNRINESNSFANVDIKEKNQDHNKKTPLS
                     NILNQKNSPSFNHSIEPKYNKETYLSEDQSNKGKQSLNQTNNKNKKNIFKLPLAFIFS
                     KITKQDENKKVEERIFETTKKESRDSLSNIVNKNKKNDRK"
     gene            complement(8876..9046)
                     /locus_tag="VF222_00880"
     CDS             complement(8876..9046)
                     /locus_tag="VF222_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HEX7141010.1"
                     /translation="MTKGTTSMGKYTRKKVHIRCRRCGHNSFHKRGKACSKCGYPDPK
                     MRKYNWIKRQVM"
     gene            complement(9088..9315)
                     /locus_tag="VF222_00885"
     CDS             complement(9088..9315)
                     /locus_tag="VF222_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HEX7141011.1"
                     /translation="MSIDMAVKVLDESFGKIVLIKLKGGKVIRGTLHGFDQHMNLLLD
                     DAVEIVEGKSSDLGTIVVRGDNVVIISPPPV"
BASE COUNT         3127 a         1447 c         1405 g         3436 t
ORIGIN      
        1 agcacttttt ctttaataat caagaaatat caattccaaa ttttttccag gcttaattat
       61 aactctttga catactgtct aattcataca ttgcataatg ctttgcatca actgttttag
      121 ctcttccagc aaggatgttt gcttcaaatg gcttcattga tctacacatt atattataat
      181 gggctttcct taaccacata aaatgcaaat ctttgtcttt agcctttaag taagaatgtg
      241 ctgatttgat atgaggatta taatcaaaaa aagataaatt tctaaataat ttccatatag
      301 ttgtaggaac aattgcaaaa taacactttt tgtgtcccct atgccattga attaaaatta
      361 tagatgttcc atttggtttt ttaaatacat gtaatttttc acaagaacat ccaccaagat
      421 ttgaacaaat ttcctttctg tatatataga ctaattcatc ctctctaaga ccagaaaaca
      481 ttgcaaattt gactataagg ccaatttctc cttgtaattg caatactgtt cttagttttt
      541 ctgctaaata attatcatca acaatatata atttgctgtg atcattattt cctgcattca
      601 aattatacct tcttataatt ttaaatacaa gatcaatgca actatcgtca ttgtatttgt
      661 aaaggtaata ttgtccaaat tttctaaatg aatccattat ttttgatcgt attcgtggag
      721 aaagcattct caattcttct cccttttggg gcgtaaagaa ttgatctttg aatcttctaa
      781 aatagctaat cagatcttta caagtctttg gatgtaaacc tcttatattt tgataatagt
      841 tatacatatc atcaagatca acatttttat aaaaatcacc agcattaccc acttctaaaa
      901 ttgttttttg tccatttgac tgagtatttc ctatcaaatt gtctatttgt ttgtcttctg
      961 taatttgagg aaatcttcct gctataaatt catgtactaa ttcatttatg ctattgaaac
     1021 caaataattt caatctttga ttaaaaacaa taagatcttc tggttttact ctagccgtta
     1081 agctttttct attatcctta cttatttttt gaagaacaaa ttcttttgaa tttttagtat
     1141 gtaatttggt attttcattt tgcaaattgg acattatcta gcactattat agcttttcaa
     1201 cctaataagt gttattgaaa ccataaaagt ttcaatataa taaagtagta taaagttgac
     1261 cattaaaagt tcttggtcaa caaagtctac aaaaagttga ccataaatac aataacggct
     1321 ttcatgtcaa ccatgaaatg gtcaacataa tggtcaactt tttatgtttt tatttggaaa
     1381 aatatgcctt attttggtca acaattacta gttaatgaac attaaaatca gcgagtattt
     1441 ttatcgaaac aatttattgg actgggcggg atttgaaccc gcgacctttc ggttgcgaac
     1501 cgaacattac taccacactg aactaccagc ccttaaaatt aaatattata ataaataata
     1561 aatatgttct aaactagtat actagtataa cttatcactg tttggttatt tttttaatta
     1621 tttataatta attgtatcta ttttataaaa ttttattcat tttaatatat taacagaaaa
     1681 atatctagtt attgaggaat tatattatat tttatttaat aataatcgaa gggaaaaaca
     1741 ttatatatca tgagtgaaga attgcttata atgaaactac taactaccgg tttaatgatg
     1801 ggtatgcttg tagctggact attattaagt ccatttgtgt caggtaatcc aattattaac
     1861 aaagtctttg cacaagaaga agaaggacct gttaaacagg tcttactggt tgcaaacgaa
     1921 aatcaacttc aaattgcacc agataatgct ttacatccag gcggtattga atacgctgca
     1981 atgaccttta atggtactat cccaggccca gttattgctg gtgaccaagg agatgtttta
     2041 aacattacaa ttagaaatga tggtaaaacc atccacagtt tagacttcca cgctggattg
     2101 ggaccaagta acgtattatc tggtaatatt gaaccaggtc aatctaaaca cgttaccttg
     2161 catgctgaaa atccaggtgc atggatgtat cactgtggtg ccgatggctt aaatggtgta
     2221 tgggaacata ttgccaatgg tatgtatgga gcagttgttg ttcatccagc tgatgaacaa
     2281 ccagcaaaag aattctatct ctcattcgga gaaaatttca acacagcaga taaaggcttg
     2341 tttgtaggga caaatggaac aactggaaca tttgatctta ctaaatttgc aacagatcag
     2401 ccagaccttg tattgactaa cggtatggca cacaaatatg ctccaggaat aggtgagaaa
     2461 gtaaagattg tattgaatga aaatccagaa atctttaagg ttgcccctgg tgaattaacc
     2521 agatggtata tcttgaatcc aggtcctaat ggtggggtat cattccactt tatttcaggt
     2581 atgattacaa ctagagatgg aagtactcca gatggtaaag actatggaac tcaagacgtg
     2641 atggatgaaa catggtgggt cccaccaggt tctgcttctg tatttgaagc tgtcttccca
     2701 gaaaccggag cttatgttgg tgtagaccac aacatgaatc atgttgtaaa aggtgcagca
     2761 tttatcgtaa tgggtgatac aaactctaca gctaaagacc aaccaccagg aactgcagta
     2821 gctccaaaag gtagcgaaag tgcagtagcc gattggtcaa aaccaaaacc atatgaaact
     2881 ataattgcag aagctagtgc tgaaactggt gatataacag ctaatgcaac cttagcatat
     2941 gctcaagaaa caatatctat gccagcaggc gctgcaaatc caagcaataa agaattttat
     3001 gtccctgctg aaacctctgt acctaccgga acaactgtta catggtcaaa tgatgatact
     3061 acaattcata cagtagtcca aggaagtccc caaggcggag caggcgaaac accagcattt
     3121 gattcaggta taattgcacc tggtgccaca tttgaaaata catttgatac agcaggagat
     3181 tttgactatt actgttcttt acacccattc atggtgggca aagttacagt aacctaatct
     3241 tttttttaga tacaattagt ttattattat aatatccgta aataactttt atctacttaa
     3301 tcttcttaca gtaacataat taattagctc ttgtcttatt atttagggta atgcaaaatt
     3361 cctgtgatct attaataaaa aatggaattg ttgtaattcc taaggttgga gttctcaata
     3421 ctaatataat gatcgaaaat ggaaaaataa aagagctttc cagatcatta aataacatta
     3481 gttatagcag atctattgat gcaacaggaa aatatattct tcctggatta atagaccctc
     3541 atgttcatta tggtgtttat acaccaattg aagaagcagc aagaaccgaa tcaaaatcag
     3601 cagctatagg cggagttaca acaatgatca gaatgttaag gttatattca gattatagga
     3661 gtaatattaa aaaacagatt gaagctagtt taaagaatca tattatcgat ttctctatcc
     3721 attcgtcgct tttgatagac caacatctcc aagacatttc ctatttgata caggaaacag
     3781 gcataaattc cttcaaggta tacatgaacc ttggatcaaa acttaatcaa attcatatgg
     3841 atttaaatcc aggggaaaaa gatattcgca gtggcaaagt agaaattact gatggttttt
     3901 taaatgaaat tgttaaaact accgcttttt ttaatagtat gcttttagta catgcagagg
     3961 atgcagagat ctgttataaa caaataaaaa aagaacttat tgataataat aaaagtaata
     4021 acaatgattt attattatta gaaagtaaaa aaaataccag gcaagcgaca ggtaacatat
     4081 ctctaaatgg aaaatcttct tttaaagaaa atattgatga aacaacagaa gaggaggagc
     4141 aaaaaaaaga tagtaatact gacaagaaaa aatttaaagc gccaaaagac caaaaattag
     4201 aagagaattt acttgaattg tggtctcgat gtaggccaat tcattcagag gtaaaaagta
     4261 tacaaaaaat tgctgaactt ggtagaaaat ataattcaga tatttatttt gttcatatag
     4321 gatcgagtgc tgctatagat gcaattatcc aagagaaagt aaaaggacaa tgtaatctgt
     4381 atattgaaac atgtcctcac tatcttactc atacctatga ttataatgat ttaaaaggca
     4441 aggtagttcc gccattacgt tctaaacatg acttgcaaag cgtatggtat gctctacgaa
     4501 atggaattat tgatacaata ggaactgacc atgttgctaa tcgattatcg ctaaagttgg
     4561 atcgaaatgg tgatttgtta gaatcattat caggatttcc tggaattgct actatgctac
     4621 cagttctctt gagtgaaggc gtaaacaaac gaagaataaa cctacaaaga gttacagaag
     4681 ttacaagtta taatacctcg agaatatttg ggctttatcc tcagaaaggt tctatacaga
     4741 aaggatccga tgcagatttg gttattgtgg atcttgatct taaaaagaaa gtcacacctg
     4801 atctattaca gtcatattct gattatacta tttatgatgg atgggaattg tgtggatggc
     4861 ctattacaac tatattgaga ggaaaggtaa taatggaaaa caatttggtg gatgaaaatt
     4921 cttatggata tggaaagttc atccagcgat tcaaaaatta aggagagata aatctaagat
     4981 ctccgtagtt tagctctaag atgatttgac agaatattta ccttcgctat tgacagaata
     5041 atagtgagta ttgttttttc ttgagaaaag gccaacctct ataactcctg ggatattttt
     5101 tatctctttt tctgccttct ctacgtcaag aatactagaa aattctgtat ctaatattat
     5161 attaccattt tcagtaataa agggatagcc tttttggata gtccgaatta caggttttgc
     5221 tgacaaatta agataacttt ttttatctcg tctattattc atttgagaac catttgtaat
     5281 tccatctttt cctcctcctc ctataatttc catagattcc agtcggttcg ttactgtaga
     5341 tcgggcaaag gggtggactt ctataggaat aggtatggtg aatttaggaa cgaatttctt
     5401 tgaatccgct agaataattt ttttctttgc tgaatacatt ataattttct ccttgaaaag
     5461 tgcccctcct cctcctttaa tcatatccag gttatgatca atttgatctg ctccatctac
     5521 taccaagtct agaatattta ttttagtctc atctgtaaat tttaatccag ccttttcagc
     5581 aattattttt atttggatag aggtagggat aaaattgaac ttctctttgt tagaaaccgt
     5641 tgatattttt ttaacaaatt cagcaaccgt gcttccactt cctaatccta taattatatt
     5701 ctctttttta aaattctttt tatcaaatag ttttaaaaaa tgatttttaa taatattttc
     5761 aaacataggg actaagtatt cactattatt gtctttcaat accgcattcg gaattatggt
     5821 gcatgtgtac gttttattat tctttatcta actttataag ataaaaataa aataaataat
     5881 cttcaacaga taattgtagt cgagaataca tattgaaatc gttgtaataa ttgaatagaa
     5941 attgataaaa ttatgaataa taatattttt taccagtgtg aacattggaa ctcggttttt
     6001 ttattaataa aagaatttaa gaattatatt tttaccttaa tctatttggt taatttcttc
     6061 aatattgttt tcatatttat tatcgcttaa aatcaaattt tagattctcc aatagtaaag
     6121 acgcacaaac aaacggtcga tcagcataaa gtcataatca actggtaact aatatccaat
     6181 tgaggaaaag atgtgaaagc aacactaata ctaagtattg attgtatata tatataattt
     6241 ttttatatta tctatacttt aaaaatgatt tgataaaatg tttctgtaat ttatcatttt
     6301 gttactattg atcttttctt cctattttta aaacaatata aatattcttt gtgtgtcatt
     6361 gataacacaa tattataaag aatatttatt ttaaaatact aaattattga aatatatatt
     6421 attattattt gtgttataac ttagatccat aagttgcaag cttcttgtaa aaatgatttt
     6481 ttgaagatgg aatggtttac ttctacttta tataaattac tactctaata ataataataa
     6541 taataataat aataataata acaaaatcat atggttatta atactctatt attttttgtg
     6601 taccatgctt atagttttat aatctagttt tgttgtagca ataagaaaat aaatcattat
     6661 taacaatttg gttcaatttc agttcagaaa ttaaactgca taaatatttg ttgctttgct
     6721 ccaataatca cttatcaata aatatacaaa ttcttttatt aagcatcttt tctatatcaa
     6781 tagagaatct taatgttcgg tagaattggt tcagtgatat tgcttgtgga cgatttggaa
     6841 cggtcggccc aattttataa agatacaatt ggtttaaaac ttaaagtaaa aactactgat
     6901 tggatagaat tttttgaaaa agggactacg cttgcattgc atccaaggaa aaggagaaaa
     6961 gatgtagatg atgatcgtga tgggcatccc aatgttcttg taggatttat ggttgctgat
     7021 ttggatcaag tagtcaagga tctaagagca aaggatgtga gattttttaa ggaaccaaaa
     7081 acagagccct ttggcaaaca cgcaattata ttggatcctg atgatcatct tatatctctt
     7141 gctgaaattg aatcaacaag atctaacgag gaatttgatc ttatcggatt tttaggtgca
     7201 gaataacacc tgtagtactt tatataggta ttttaaatca tctaatagag aaaataaaat
     7261 aatttatttt ctatcatttt ttttattttt attaacaata ttgctaagcg aatctctact
     7321 ttctttttta gtagtctcaa aaattctttc ctcaactttt ttgttttcat cttgtttggt
     7381 gattttagaa aaaataaatg ccaatggtaa tttgaatata ttttttttat ttttattatt
     7441 agtctgattt aaggattgtt tacctttgtt gctctgatct tctgataaat atgtttcttt
     7501 attatattta ggttctattg aatggttaaa acttggacta tttttctgat tcaatatatt
     7561 cgatagtggg gtttttttgt tatggtcctg atttttttcc ttaatatcta cgtttgcaaa
     7621 agaattcgat tcatttatcc tgtttagatt cacatctatc tcattattta agcctccttc
     7681 cacagcgatc tttcttagag ggtcaaatgt accgttgttg gtagtcacaa tctcattttc
     7741 tgatttaaca tcccgaaaac tgaatatttt tggaatgttc aaattctttt cagtcaagtt
     7801 attaacatat tttttatcat tttgagtgct taaatgctct ttatcattaa cttccaattt
     7861 ctcctggaag ttgtttcgta gatctttatc agtaaaagta aatatattgt cctgtataac
     7921 tttttttgtg tcgggttgat ggtctatttc cattttgata tcgcctattt tatcaagtct
     7981 atttaatgca actaaaactt gatcttttag gtcatcaatt ttttctctct cagtctctat
     8041 catcatctct tttcttttat ggcttggatt atttttgtag cttgttaatt ctctaaggct
     8101 cagattagca tcaacagttt cttcaatatc aatgaaagta ttatcaatat tctttaaatt
     8161 gttgtcattg gttttattta tccgatcctg atttgccatt tctttaactt tataattagt
     8221 tcctattttg aagttgattt cttttatctt ttcgtcaata tccgaaattt tgttatctaa
     8281 tagaaaagct aaatcatctc taagaataat taatttggta acatctattt ctgactgaat
     8341 tttctccatt ctttcattta acttttttaa ctgctcttta tactttagta gaaggcgatc
     8401 tttctcaaag gaatcaattg atttgttttt agaagcaagc aaaatattct ctatagcatg
     8461 ggttgccagg tctttctcga attggtatat ctttaactca tcttccatta actgggaatt
     8521 tttttttatt aaatctggtt tggtttttag cacgtcttct aattctttta ctttgttttc
     8581 ttcttttgtt gcagaggaat tatttgactt ggaactattt ttctttagat aaaaaataaa
     8641 ggaggagaag attctagtat agtataatcc aaaaataatt cctccaataa tggatatacc
     8701 agttattatt aataaaaaag agtcttcgtc aaccatttta attatatatt tatactaaat
     8761 atcgatatat ttaattctaa aatagcccta atcaatatac aatcgtaaaa caaaagtaaa
     8821 gaataatgtt ttaattaaca attatacaag atttttttta ttttatttta tttcattaca
     8881 taacttggcg tttgatccag ttatattttc tcatttttgg atcgggatag ccacattttg
     8941 aacatgcttt gccccgcttg tggaatgaat tatggccaca tcttctgcat cttatatgta
     9001 ccttctttcg tgtgtattta cccattgaag ttgttccttt tgtcatgttc tcatatcaac
     9061 ctccttgtta cagatactcc ccttatttta tactggcggt ggagatatta taactacatt
     9121 atcccctctt actacaatag ttcccaaatc tgacgacttg ccttctacta tctctacagc
     9181 atcatcaagt aataagttca tgtgttgatc gaatccatga agagttcctc taattacttt
     9241 accacctttt aattttatca gtacgatttt tccaaaactc tcatccaaaa cctttacggc
     9301 catatcaatt gacaatacta attctacctg ttactaacaa atgaatatta gttatatatt
     9361 aaaatttcat tcaaagtcac tatttttatt agtgaaatac tacaataaaa aaatt
//
LOCUS       DASVEV010000019         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_5058, whole genome shotgun sequence.
ACCESSION   DASVEV010000019 DASVEV010000000
VERSION     DASVEV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5058"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            629..1165
                     /locus_tag="VF222_00890"
     CDS             629..1165
                     /locus_tag="VF222_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7141012.1"
                     /translation="MYSGAVDVLGTLTEKLKQQINDTQDKYESFIDATHVYKQGKMNE
                     KEYFAKIGDYLVSTSALGFLAIRVILEIKSAIDKSTSMKNATGGMASPPSPQAGFGIG
                     GFVGTGGAVGGGGEKYVMPAPKQQEPTFKPEDIEPERRSSESSGKEKTTTITKNCIFC
                     GSEIPKQAKFCSKCGKLQ"
     gene            1173..1331
                     /locus_tag="VF222_00895"
     CDS             1173..1331
                     /locus_tag="VF222_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141013.1"
                     /translation="MYNDILSIGKTIIECQSLDVVIFSLSLIGKNWQDCVLKVSVTNN
                     WHEFTMEK"
     gene            1337..1540
                     /locus_tag="VF222_00900"
     CDS             1337..1540
                     /locus_tag="VF222_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141014.1"
                     /translation="MVRSNNKMNYQESIGFVNIPRSCVAFSRSKRKTIIVGDRNTLMK
                     NRFLAKSIDTVIQKEGFISWTKD"
     gene            1649..2212
                     /locus_tag="VF222_00905"
     CDS             1649..2212
                     /locus_tag="VF222_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX7141015.1"
                     /translation="MPQTKPIVSIENVVASATINQKVNLNQITQIFPDVEYHPDQFPG
                     LVFRLKSPKTATLIFSSGKMVCTGAKSEDQAIKAVKSVVQKLNNGGIQIENEPIIEIQ
                     NIVASVSLGGKIHLELAARVLPRSMYEPEQFPGLIHRMSDPKTVILLFASGKLVCTGA
                     KKEAEVYRAVHNLHNLLEEKNLMIYEG"
     gene            2577..2855
                     /locus_tag="VF222_00910"
     CDS             2577..2855
                     /locus_tag="VF222_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141016.1"
                     /translation="MINKYCKTLRGLDRHIITTFRNRLSDLEIAIHTLNDIPICDKSN
                     ITTNGSTHDKNFILSIIKVLKDIDWLAQDKNGKYSRTMKIQVNTHFTN"
BASE COUNT         1369 a          520 c          573 g         1187 t
ORIGIN      
        1 tattaaacta aatcttaaac ttacatgata gactattcaa gaatgtgatt atgactacag
       61 taggctgctg cccaatattt aaaaaatctc tccgatcata aaaatactaa agatgttatt
      121 gtaccagttg gaaagcaaca aacagaatta ttgaagccat gcctaaaaag ttcttacaaa
      181 gtagcgttgg cacagtattc tcattctgat gttgtatttt tattttctac agcaaatcgc
      241 ctggagtggg actcgaacct cgaagggtta tttcggggtt tataaaattt gatataattc
      301 aaggacattg aatcctcctt caccatatct tctattctct taaaccaatc tctatctttt
      361 tcgtttgcat tctcaaaatc atatcaaatt tttaaatttt ctaagattga aatggaaaaa
      421 tttaattata aactgcaaat tattttatta aagaaagaag aataaattaa aaacattatg
      481 gtgatgtttc cttgaaaaat atccgtgatt ttgaagaggc aagagaattt ctatgttgac
      541 gttaccaatt tttttaggca atacttgagt aatttcagaa acgttcaaac ttttattaac
      601 gccgcaatct acatctttaa caacaataat gtattctggc gcagtagatg ttctaggaac
      661 actaactgag aaattgaaac agcagataaa tgatactcaa gataagtatg agtcatttat
      721 cgatgcgacc cacgtctaca agcaaggaaa aatgaatgaa aaagaatact ttgctaaaat
      781 tggcgattat ctggtttcaa catctgcatt gggttttctt gcgattcgag taattttgga
      841 aattaagtca gcaatcgaca agagcacatc aatgaaaaat gctactggtg gaatggcttc
      901 tcctccttct cctcaggctg gttttggaat agggggcttt gtaggtactg gtggggctgt
      961 tggcggagga ggagaaaaat atgttatgcc tgcaccaaag cagcaagaac ccacattcaa
     1021 gccagaagat attgagccgg aacgacgttc tagtgagagc agtggaaaag aaaaaacgac
     1081 aacaataact aaaaattgta ttttctgtgg atcagaaatt cctaaacaag cgaaattctg
     1141 tagtaaatgt ggtaaactac aataattaaa agatgtataa tgacattctt tcaattggta
     1201 aaaccattat tgaatgtcag agcctagatg ttgttatttt ttcgttatca ttaattggta
     1261 aaaattggca agattgtgtt ttaaaagtta gtgtgactaa taattggcat gaattcacta
     1321 tggaaaaata atatcaatgg ttcgatctaa caataaaatg aattatcaag aatctatagg
     1381 atttgtcaat attccaagat cgtgtgttgc attttcgaga agtaaaagaa aaacaatcat
     1441 agttggggat agaaatacgc taatgaaaaa tagatttttg gctaaatcta tagatactgt
     1501 aatccaaaaa gagggtttta ttagttggac taaagattaa tattaattca ttataaacaa
     1561 ttagcatact cttattttat tttaaccata tcaaagattt atgtctactt atatatcctt
     1621 atatatttta tataatgcct acttcattat gccgcaaact aaacccattg taagtattga
     1681 gaatgttgtc gcttctgcaa ctataaatca aaaggttaat ttaaatcaaa tcacacaaat
     1741 atttccagat gttgaatatc acccagatca atttccgggt cttgttttta gacttaaatc
     1801 tccaaaaact gctactttaa tttttagctc aggaaaaatg gtatgtacag gagctaaatc
     1861 agaagatcaa gcaatcaaag ccgtcaaaag tgtcgttcaa aagttgaata atggaggtat
     1921 ccaaattgaa aatgaaccca taattgaaat acaaaatatt gttgcatccg taagtttagg
     1981 aggcaaaatt cacctagaat tagctgctag agttcttcct cgcagtatgt acgaacctga
     2041 acaatttcct ggattaattc atagaatgtc agatcccaaa acagttattc ttctgtttgc
     2101 aagtgggaag ttagtttgta caggggctaa aaaagaagca gaagtatatc gtgctgtaca
     2161 taatcttcat aacttattag aagaaaagaa ccttatgata tatgaagggt aattattatt
     2221 agattacaaa aaggaaacaa ttatgaattg gattaaaaat aaattatttt taaatcaatt
     2281 tagatattat aaaattttgt ttgttctaaa atgttctcaa tgcaaatatt ctactaaaaa
     2341 taatgtggat agaggagttc gccttttttc tgatcatcta caataacaag atcctttatt
     2401 agttaaatct tatgacaagt tttaccaaat aatagagtta attaaacttg taaaatagat
     2461 atgattaaga atagttggaa gaattaagaa atctatccag agaaaaacag gaaggatcta
     2521 tggaatttac atcaacaact tttaaaggct taaatgcatt ggcgatatag cttatcatga
     2581 taaataaata ctgtaaaaca ttaagggggc tggatagaca tattatcaca acatttcgaa
     2641 atagattaag tgatctagaa atagctatac atactctaaa tgatatccca atatgtgata
     2701 aaagcaatat tactactaat ggatctactc atgataaaaa tttcattcta tcgataataa
     2761 aagttttaaa agacatagac tggttagctc aagataaaaa tggaaaatac agcaggacaa
     2821 tgaaaattca agtaaatact cactttacta attaagatgt catgaaatga ctggtatatt
     2881 cttggcgggg acagttctaa aggttcaaga tcatttcata ttttgattct caaaaaacta
     2941 tcatatttat atacttagta tactgatgag ataatgaaaa cacctcttat acaaagtcta
     3001 ttcacaaaac ctcattcacg tattagacaa atttaaagtc attattacat aaattaacat
     3061 ctcaatcagt tattggtgaa gattcactat tttgttctaa ctgttgaact ttattcaatt
     3121 tcaaagatga tatcataaaa ttaatattaa aaaattatgt tcatgattat aaaatatttt
     3181 actatttaac tcaaaccatt taattcgttc atggtatttt ttcagtgatt acacaataaa
     3241 tgattttaag aatttagaaa ttttaaggca tttttataac atgaggttca tggaggctca
     3301 tgacatacac atgtcctcta gtttggtttt tcagtccaaa gaactccaaa cagaaccctg
     3361 ataaaacgta ttatacaaca ctattaaaaa aaggttcttt tctttcttct attatattct
     3421 ttcaatgata tgacatgggt caaagacact agattgtgcc atacctccat caacggacag
     3481 tttatgagag taatgaatag atagaacgat aaggtaaaag aaaatgcatt agcttattta
     3541 tctgatcagc tattagtttc caaaaattta ttgatgatta gagtatttaa ttaaaaattt
     3601 tggtaataat aaaacaaaaa caacaataat attatagtta atcaaaaag
//
LOCUS       DASVEV010000020         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1559269, whole genome shotgun sequence.
ACCESSION   DASVEV010000020 DASVEV010000000
VERSION     DASVEV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559269"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..853
                     /locus_tag="VF222_00915"
     CDS             521..853
                     /locus_tag="VF222_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141017.1"
                     /translation="MMITSITRKPSKVFLVTLLVAGIITISSQSFMIGAQAQQDPIVD
                     VEKLKCSNDNFNIDVTNSQVQSEVANKIQSLLAQEAADNSGKQSTDEDIVLVCTNNNN
                     LQVVSSVG"
     gene            1268..1762
                     /locus_tag="VF222_00920"
     CDS             1268..1762
                     /locus_tag="VF222_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141018.1"
                     /translation="MQKSAFTTVFLIAILLSGAISTALPSMIDTEEYHESYNKAYAES
                     NDVNSPKKIKIIDCNNRNINAPEIEEFSTIEPTLNQAFNSNIDKVNNESQVIYLEPDT
                     KIIFNCNNQNHYLQPIETVADVAVTTGSLLPSIQQMNPTIPQNSLPSGDPMLQLQSSL
                     SPIF"
BASE COUNT          846 a          337 c          277 g          741 t
ORIGIN      
        1 ggtgggggaa gcacagatag tattcttaac atatgcttta atatcaataa taatgatcta
       61 ggtaacgcca catttacagg aggaacaggt caagcccaaa ctggtacagg cggagcggat
      121 actacaacag gtgatactac aataaactag aatagaatca tcatcatcat catcatctcc
      181 tcttattttt tcttcctatt tttatttttt aactgaaaat tcaacagatg cattatgatc
      241 atagaataat aaacataatt tgctttcttt tttttataat cttccaattt ttaaataaat
      301 tttagatcta ttctgtctaa tagatatcat taccaaaaaa catcatcgct tgaatttgta
      361 aaacaaaact tggacgaaat ttaaatgaaa atcttctctc agatatctga tatattattc
      421 tacattcttc tatttttagg ataaattaaa atataatact ttatctctca ctatcaatca
      481 ttcgaattcc agatatactt tatattggta gtagatttaa atgatgataa catccataac
      541 aagaaaacct agtaaagtat ttttggttac actattagtt gcaggaataa tcactatatc
      601 ctctcaatcc tttatgattg gagcacaagc ccaacaagat ccaatagttg atgtcgagaa
      661 attaaaatgt agtaatgata atttcaatat cgatgtaaca aattctcaag tacaatctga
      721 agtagcaaac aaaatacagt cgctattagc ccaagaggca gcagataata gtggaaaaca
      781 gagtactgac gaagacattg tacttgtttg tacaaacaat aacaacctcc aagtagtatc
      841 ctctgtgggt taaactatat aattggacag gaaaactaat ttttattttt ttattatatt
      901 atagtttcca ttattttgcg ggtattttag taattataac aaaataattt agacagatat
      961 tttggaatat atgaaaaaag gtataaatat ctttttttca tccttcagtt atgtcaatac
     1021 tatataaaat aatatctata tggttttgta aacatttcgt cggatttcta atgctgtcaa
     1081 aaaacgtcgg ataaaaaatt tttaaactat tttgattgat gaaattgcat aagaattaca
     1141 aactataaac attcttagta tgatattgca ataatgctaa ataacaatta acttaagaca
     1201 aacaatagag taaacgaata ttacaatttt acgcagagta aataaacact attcaattat
     1261 attcgttatg caaaagtcag cttttaccac tgtattttta atagcgatat tacttagtgg
     1321 agctattagt accgccttac cgtcaatgat tgatacagaa gaatatcatg aatcatacaa
     1381 taaagcatat gctgaaagta atgatgttaa ttcacctaaa aaaataaaaa taattgattg
     1441 taataatcga aatattaacg ctccagaaat agaagaattt agcaccattg aaccaacttt
     1501 aaatcaggca tttaattcaa atattgataa ggtcaacaat gaatctcaag taatttatct
     1561 agaaccagat acaaagataa tattcaattg taataatcaa aaccattatc ttcagccaat
     1621 cgagactgtt gctgatgtag ctgttacaac tggctcccta ctcccatcaa tacaacaaat
     1681 gaatcccaca attccacaaa atagcctacc ctctggcgac ccaatgctac aactacaatc
     1741 aagcttgtca ccgatatttt aaacagtgtc tataacacct ttttttttat tttctattaa
     1801 taaaaaattt taaactatag tcaccactaa aaattataac tttaattgct caatgatatt
     1861 atggcatttg actatattgc tttaataaaa attaattaaa aacacagtaa gtgtatttat
     1921 tccatctatt gtcgtaaatt atcaaaacct ttatggtgtg aattattact tgaaaatcta
     1981 aagttacttt ttatctcaat ttgtattcta ataaactata ttcaaatcgc caacaaggta
     2041 attcagagat gaaaatgttt cagaaattag agttgattat tgaggctaaa taatttcaaa
     2101 ataattatag ttttaaatac accttaaagt tctaaagaaa ctaataaatt agctgactcg
     2161 atcgcctagt gtattattat atagataaga aataagaaga a
//
LOCUS       DASVEV010000021         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_5245, whole genome shotgun sequence.
ACCESSION   DASVEV010000021 DASVEV010000000
VERSION     DASVEV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5245"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..641)
                     /locus_tag="VF222_00925"
     CDS             complement(498..641)
                     /locus_tag="VF222_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141019.1"
                     /translation="MNLNISIVRQPQDNLSPNVIYNILSYHNINKDEPKKATGQSGVD
                     SSM"
     gene            complement(1137..1301)
                     /locus_tag="VF222_00930"
     CDS             complement(1137..1301)
                     /locus_tag="VF222_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141020.1"
                     /translation="MEGSACAFISCQKRPTSQCLQCSSYYCSEHIQTHLHRLSPDSIQ
                     KDKDDLHKMR"
     gene            1593..1757
                     /locus_tag="VF222_00935"
     CDS             1593..1757
                     /locus_tag="VF222_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141021.1"
                     /translation="MENDKDIKIETDEDVGLDDKIKAGARALGKKIEDPDRDTGSEYE
                     AEKTKERVVD"
     gene            1763..2131
                     /locus_tag="VF222_00940"
     CDS             1763..2131
                     /locus_tag="VF222_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141022.1"
                     /translation="MIQSSTKTAAVIVITVIFIATIFAFSSTVTISAFAQAESMNQSM
                     NTAGDSTNQTMKNMSQESNQTLKEMSQSANQTGEAIQQNASNFGSNISEGAKELGTNI
                     TEGVKNLAENIGEKLQDLAK"
     gene            2423..3322
                     /locus_tag="VF222_00945"
     CDS             2423..3322
                     /locus_tag="VF222_00945"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX7141023.1"
                     /translation="MESFHRENNDNSMVHEENKNDNKKKNPEVTPIIISPKKSKESRM
                     TIRAHKVHQGDSITSKIGHLFGSVKEKTTDYVEKVKIKHQDINSFATTKVQTETTIEE
                     EGQQFVIPIIEEKYNLSKKILSEDVKIEKRWIIHDEKIHLPIAYEKIFVDDKELDSYS
                     KEDILTQIKDKIINFQYIGENDDDENKIEKEKKEDIKHQQNEKDRRQVRVKEYEIPLF
                     NEEKTNSTSYTSNNEILNDKPQKVIPLYAEQLVITKKKVKIGEILITKKKVIEDKKVN
                     IDIVKERVTIEYPNGNKERLTEN"
     gene            complement(3891..4076)
                     /locus_tag="VF222_00950"
     CDS             complement(3891..4076)
                     /locus_tag="VF222_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141024.1"
                     /translation="MEEKLKFVVHGRKEGNKFVLEKITNYILKNLESFDDKTIIVTFE
                     MPVTNNEIKMDSCILIN"
     gene            4681..4911
                     /locus_tag="VF222_00955"
     CDS             4681..4911
                     /locus_tag="VF222_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141025.1"
                     /translation="MSNKSNDYKSDAQNDEIKSIVLGLFALMGFLIIIYSLFMGVVGE
                     PLASVADTVFVAVIIGAFTLVGIIVGRVFLKK"
     gene            complement(5130..5801)
                     /locus_tag="VF222_00960"
     CDS             complement(5130..5801)
                     /locus_tag="VF222_00960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7141026.1"
                     /translation="MINHGYNNDNNANANKKKVLDVLSEIEEIAKKDSLPSIGPIKGE
                     IIEDIIKKYKPKRILEIGTLHGYSAILMGNFLLDKDNDSNDNTTKEIIITCLEIDKNL
                     VNIAKKNIEKAGLSDRIKVITGDALKIIPTLNNHYRFDLVFIDAVKNHYLKYLKLVED
                     NNLLNKKSVVVADNVLIYEKEMKDYLDYVRNSGRYNSYTTETTLEFTKNVKDALEVSI
                     TANMD"
     gene            6007..>6468
                     /locus_tag="VF222_00965"
     CDS             6007..>6468
                     /locus_tag="VF222_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141027.1"
                     /translation="MNKTRLSNPAVILVVLILLSSTMVIFFPLVSEQQQQQEGKPGSI
                     LKLAKANVSIDIPLSKGYIDGNIAYFIATDASDKHAVSSITNNTGFPVNYAPVLNNTS
                     ESIRGQGYVFLNGVQGEAPGGFQLPVANAVPGDKDYSPLWQTNFVKWNDNAT"
BASE COUNT         2474 a          857 c          895 g         2242 t
ORIGIN      
        1 ttttttgata tactataata tctaaatatt aatttttata ataactccac atttcacaaa
       61 taatgaaaaa tacatcttga tattattctc tttattacca tacattagac tcttatttta
      121 gaatcgatat cggtataatc ccagataaac taagttcaat agatatttta tttatagaga
      181 atattttaaa gaatatcttc aaatttatct gtttgattat gatgtctcat tcacagtttg
      241 agataaagta ttatttgttt ccatggattc atctatttct gacgttgtaa ttaaagaaga
      301 atcatcaatt tctggtgttt catttttttc ttgggaaaag tgattcctga atttatagat
      361 agtactccta ttataagagc tgcaataaat gttatgagaa aatatgtgtg tttcataaga
      421 ttgtaataat aatataatct attttaaata gtgtcttatg aaaataataa aagctaacta
      481 caagttagtg aatcctacta catggacgaa tccactcccg actgacccgt agctttcttg
      541 ggctcatctt tattgatatt gtggtaggaa agaatattat aaattacatt tggagacaga
      601 ttatcctgag gttgtcgtac tattgagata tttaaattca aagttcaagt attttagaaa
      661 tgtattgaaa tgataagaat aatatcataa ctactcttgt taatcttggt taataatttt
      721 atataaatga ggatcatata tttgattata gtctttggta atatatcaga gcattataga
      781 tgtattatgg agaaattatt gcatcaatat tagttaaagg ataagattga gtctcgaccg
      841 tagaattgtc aataatatag ggttttatat ttatatttat cttagttttt aagtaacaca
      901 tttgaaaaaa ggaaaatgtg aaagctataa ttatagatgc agagaaaaat aatcctagta
      961 aattataaaa gatcctacaa gatttaaaag gagaggtgct tcaactgcct atctattcat
     1021 aggttatttt atttcataat aattaaataa taaattgaat aagaaattag atccaacctt
     1081 agtgtttatt tttagtaata tctctaaagt gattaataat aaataaaaaa agaaatttat
     1141 ctcattttat gtaggtcatc tttatctttt tgtatactat caggacttaa tctatgtaga
     1201 tgtgtttgta tatgttcaga gcaataatag cttgaacatt gaagacattg acttgtcggt
     1261 cttttttgac aagaaatgaa tgcacaagct gacccttcca tagtattggc tctccaatct
     1321 ttcggattgt atacctgtcc catatcgtta ttataatata aaatagatat taaactttaa
     1381 gtaatgaatt tattgtttat gacatccaaa gagacggatg tcatattgac acagccaatt
     1441 tataaattat agttagaaga tagattcgtt ttgaatgaaa attaaattca ataaaatttt
     1501 attatgatat tattcctatt aatgatacat aaaattatta tttattaatt aaatctaatt
     1561 gctataaata ataatgtata acaacaatta caatggaaaa cgataaggat ataaaaattg
     1621 agacagatga agatgtggga ttagacgaca aaataaaggc aggagcaaga gcattaggta
     1681 aaaagattga agaccctgat agagatactg gttcagaata cgaagcagaa aaaaccaaag
     1741 aaagagtggt tgattgatgg tattgattca atcatcaaca aaaacagcag cagtaatagt
     1801 aataacagta atattcattg caacaatttt tgccttctcc tctacagtaa ctatttcagc
     1861 ctttgcacaa gcagagagta tgaatcagtc aatgaacact gcaggtgact cgacaaatca
     1921 aacaatgaaa aatatgagtc aagaatcaaa ccagacatta aaagagatga gtcaatcagc
     1981 taatcagact ggtgaagcaa ttcagcaaaa tgctagtaat tttgggtcca acattagtga
     2041 aggagcaaaa gaacttggaa ctaatatcac cgaaggagta aaaaatctag ctgaaaatat
     2101 tggagaaaaa ctacaagatt tagcaaagta gattaactaa caatttttat ttattactat
     2161 ttgatttttt cgaatgttcc agtttagtct ttgaggtaac ataataaacc actctataaa
     2221 ataaacctgc aattatatgg tgagtattat aattttagta gcaacaaatt attttatact
     2281 atggattcga atacactctc ctaaaaccaa aaaagactaa catcatcata tctatagtaa
     2341 taatagtatc cataacgatt ttcataataa acaataaact ggatacattc ttaaactata
     2401 ttatctataa tttattatag atatggaatc attccatcga gaaaataatg ataattctat
     2461 ggtccacgaa gagaataaaa atgacaataa gaaaaagaat cctgaagtaa ctcctattat
     2521 aatttcacca aaaaaatcaa aagagtcaag aatgacaatc agagcacata aagttcatca
     2581 aggagattca attacatcta agataggtca tttatttgga tcagttaaag aaaagacaac
     2641 agattatgta gaaaaggtaa agataaaaca ccaagatatt aactcctttg caacaactaa
     2701 agtacagaca gaaactacta tagaagaaga aggacaacaa tttgttattc ctataatcga
     2761 agaaaaatac aatctttcta aaaagatttt gtcagaagat gttaaaatag aaaaaagatg
     2821 gataatacat gatgaaaaaa tacatttacc aatagcttat gaaaaaatat ttgtagatga
     2881 taaagagctt gattcatatt caaaagaaga tattttgact caaattaagg ataaaattat
     2941 aaactttcaa tatattggag aaaatgatga tgatgaaaat aaaattgaga aagaaaaaaa
     3001 agaagatata aaacaccaac aaaacgaaaa agatagaaga caggtaagag taaaagagta
     3061 tgagattcca ttattcaatg aagaaaaaac aaattctacc agttatacaa gtaataatga
     3121 aatactaaat gacaagccac aaaaagttat accactttat gctgaacaac ttgtaattac
     3181 gaaaaaaaag gtaaaaatag gagaaatcct aattacgaaa aaaaaggtaa tagaagataa
     3241 aaaagtcaat atagatatag taaaagaaag agtaacaata gaatatccta atggcaataa
     3301 agaaagatta accgaaaatt agatataata ttaacctaaa atataacaac aaacaaacag
     3361 ctagatattt cgcatcctct cttcttattt accgatgtgc ttctaataag ctctggccat
     3421 ctctatttca acattaatct tttaccatta tcatattgta tacaggcata ctatttcctg
     3481 atagccgtga aaggttgatt aacacttatc catagagagt aaaatatgtt atagtaggat
     3541 gtattttcat attagtataa tgaagaatgc aatttattta aaatattttt agaatgtaat
     3601 ggatataata tcgagacgta tacaaattct agagagcatt ccacagcttt agaaaaatag
     3661 tcaggatttg attatacttg acttaaaaat gaattgtttt atacaaacca gtcttattag
     3721 acctaaaaat aagattgact cacttttgtt attatctagg caagaagtaa atgataaaac
     3781 aacaatgatg attatttgac atcgattact ttttactaaa ttttatttag aataatatga
     3841 gtaatggaat tttgtattac tgtattacat gatacaacat aattgcttaa tcaattgatt
     3901 agaatacaac tatccatttt tatctcattg tttgtaacag gcatttcaaa tgttactata
     3961 atggttttat catcgaatga ttctaaattt tttagtatat agtttgtaat tttttctaaa
     4021 acaaatttat tcccttcttt tctaccatgt acaacaaatt ttaatttctc ttccataatc
     4081 gagtaccaga agaaaatcat tgactgttat tatagtttgt ggagaaaatc aaaaactata
     4141 tcagtattct aaacaattat taatttagta acatttctga attgacttca taataaaaat
     4201 tagtaaatta acataatcat taacatcatt tttctatttt ataaagaaat tcaaatattg
     4261 ttattattca ttcatgttaa atgtataagc taatctttaa tattatcttc tttattacca
     4321 caacattaga cccttatttt tgcaccgcta tcgttattac ccctagtagc aggacaactg
     4381 tactacataa aatttctttt gtaaattacc tattaaagac gttattcact gtttatggta
     4441 attctttgat tactatattt aagcaaagtt ttagttaata tgataactat aatcaattaa
     4501 ccataggtat tgtatattta aaaaagatag gatagaaaaa agagaaattg ctgaagaaac
     4561 tctttacact tatagaaatg gattattaat aaagtagatt ataattatag attggttaaa
     4621 ataatttata aaaattgttt ttatattatt aaatataatg tattggcaat ggctgaaaac
     4681 atgtctaaca agtctaatga ttataaaagt gatgctcaaa atgacgaaat taaatccatt
     4741 gttttagggc tattcgcatt gatgggattt ttaattatta tatattcact atttatggga
     4801 gtcgtaggtg aaccattagc atcagtagca gatactgtat ttgttgctgt aattattggg
     4861 gcttttacgt tggtaggaat aattgttggt cgtgtatttt taaagaaata ataccactat
     4921 tttattttat ttttcaagat atgaaaggtc aatcttaggt cttaggttca tgttatacta
     4981 ttccatcaat ttcaaataaa ttaagttact aaaaatctaa tactatgtag tagaacctta
     5041 ttacatggag gactctactc ccgccaactt ccttaacatg tagatgaacc ctattgttaa
     5101 aagataatta agatttaagc aaatttgtct taatccatat ttgctgttat acttacttca
     5161 agagcatctt taacattttt ggtaaattct aaagtagttt ctgttgtata actattgtat
     5221 ctgccagaat ttctaacata gtccaaataa tctttcattt ctttttcata tattagaaca
     5281 ttatctgcaa caactactga ttttttattg agcaaattgt tatcttctac aagtttaaga
     5341 tatttaagat agtgattttt gactgcatct atgaaaacca agtcaaatct ataatgatta
     5401 ttaagagtag gaataatctt caaagcgtca ccagtaatta cttttattct atctgacaat
     5461 cctgctttct ctatgttctt ttttgcaata tttacaaggt ttttgtcgat ttccaaacag
     5521 gtaataataa tttcttttgt agtgttgtca ttgctatcat tatctttatc taatagaaaa
     5581 tttcccatca aaatagctga atatccatgt aaggttccaa tctccagtat ccttttaggt
     5641 ttatacttct tgatgatatc ttcaatgatt tctcctttta tgggcccaat tgatggaaga
     5701 gaatcctttt tggcaatctc ttctatttct gataaaacat ctaagacttt cttcttattg
     5761 gcattagcgt tattatcatt attatatcca tgatttatca agatctatat ctctcaacta
     5821 tattatatag attatttctt aattgtcaat tgattcacat atgtcattta aacacaatca
     5881 taaccttaaa acatctgaat gaactttata tttaatattt ctaaacaaaa agagtacttc
     5941 aattctcaaa gaatattatt aatataagta aacattattt aataatgttt caagatgcat
     6001 aaaaaattga ataaaacgag attatccaat cctgcagtca tattagtagt tttgattttg
     6061 ttgtcctcta ctatggttat tttctttcct ctggtatcag aacaacaaca acaacaagaa
     6121 ggcaaacctg ggtctatttt gaaattagca aaagcaaatg tttctataga tatcccattg
     6181 tcaaaaggat acatagatgg caatatagcg tactttattg ctactgatgc ttctgacaaa
     6241 catgctgtat cttctattac aaacaatact ggttttccag taaattatgc tccagttttg
     6301 aacaatacat cagaatcaat tcgtggccaa ggctatgtat ttttaaatgg agttcaaggt
     6361 gaagcaccag gtggctttca acttccagtt gcaaatgcag taccaggaga taaagattac
     6421 agcccattat ggcagacaaa ttttgtgaaa tggaatgaca atgcaact
//
LOCUS       DASVEV010000022        37323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1170547, whole genome shotgun sequence.
ACCESSION   DASVEV010000022 DASVEV010000000
VERSION     DASVEV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 37323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37323
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170547"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..79)
                     /locus_tag="VF222_00970"
     tRNA            complement(5..79)
                     /locus_tag="VF222_00970"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Met,seq:cat)
     gene            complement(249..1916)
                     /locus_tag="VF222_00975"
     CDS             complement(249..1916)
                     /locus_tag="VF222_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX7141028.1"
                     /translation="MSNTVEKSFKWKTLEHNGVAFPPNHISKGVSIKINNERLALNNE
                     QEEIVYAWAKKKDTHYINDVIFQNNFLNDFKKILPPRFHNTNNINEIDFSEAFTLVDE
                     EIKLKIREKETILKLSREEKRKLLETKKQERERLKNFYGKAYVDGLEVEVANWLVEPP
                     GLFMGRGLHPLRGRWKPRVIPQDVILNLGEDAPVPEGAWKSIVHDHSSTWLATWTEKL
                     TEKRKYVWLHDSAFLRQNNDKEKYDKARNLEKYANTVEKEIINKMINSKDLNQRKIAT
                     VCYLILKLAMRVGDEKDKDEADTVGASTLRIEHIQFPEKDNKKFIEFNFLGKDSVPWQ
                     KTLEVNSVDTKGLYDNLRLFMRDKQDNNEIFDNINSSKVNAFLRKIDTKKVPGLTAKV
                     FRTYLATKIVKEALNAPPLDVNVESSEPEKVYVAKIANLKAAIACNHKKGIDPNNPAS
                     KKALDRFAESITKKEEAIGKIKKDILSNKWTTEKQKERLKYRMEKIQYQLKLQKETRD
                     YNLGTSLRNYIDPRIMKTWLDYVALDWKKIYTTTLQRKFKWVELSKR"
     gene            complement(2017..3261)
                     /gene="cofH"
                     /locus_tag="VF222_00980"
     CDS             complement(2017..3261)
                     /gene="cofH"
                     /locus_tag="VF222_00980"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX7141029.1"
                     /translation="MNLDSIIKNIDPIISTILNKVLDGNNISEEETIELFNSSGLELN
                     MIVIVANHLCKRKKGDLVTYVINRNINFTNVCIKRCGFCAFSRDFREEEGYYLPIEEI
                     VRRAKEAEKIGATEVCIQAGLPPRMDGMLYVDICNAIKRELPNIHIHAFSPEEILYGA
                     NRANYSIEDYLKTLKEAGLGSIPGTAAEILDQEIRDKISPGRISVKDWIKVIKCAHSI
                     GINSTSTIMYGHIETYTHIIKHMSILKDIQKDTGGFTEFVPLSFVHTEAPMYNRNLID
                     GIKNGPTGIEVLKMHAIARIYFNNYIDNIQVSWVKEGPRMGQILLEAGANDFGGTLIN
                     ESISTSAGATFGQLLKPKVMRNLIHNIGKIPAQRKSNYQIIKTFSSDNYFDDELLDKS
                     DPLDFGSYQELIKLDNFRFKKN"
     gene            complement(3248..4453)
                     /gene="cofG"
                     /locus_tag="VF222_00985"
     CDS             complement(3248..4453)
                     /gene="cofG"
                     /locus_tag="VF222_00985"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX7141030.1"
                     /translation="MNSEIPISIKDKSISEILEKCQEKKSISKEDALRLLDSDDIISI
                     LKLSTFLKTRLKKDVITYSRKIFINITNLCRDFCSYCTYRKNPFESSSIMMKPTEVIN
                     LAKIGKKFHCTEALIVAGERPEDRYKEAKEWLRHLGYNSVVEYVIDISEKIVKDTGLL
                     PHSNLGNITKDEMNLLKRYNVSLGLMLESSSEKLIEKGMPHEFASSKNPSNRIEVIKN
                     AGRMKIPLTTGLLIGIGQTREEIINDLFLLKTINNEFENIQEIILQNFQPKTNTRMHN
                     FPSPPNDYFLKIVSIARLIMPDMNIQIPPNLSPSSFSSFLDAGINDWGGISPVTIDHV
                     NPEFPWPNLKELKNITESRYQRLRARLPVYPEYIDKPDFISPQIRNYVENFIGLDRLV
                     KEEYLVEFR"
     gene            4544..4846
                     /locus_tag="VF222_00990"
     CDS             4544..4846
                     /locus_tag="VF222_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141031.1"
                     /translation="MSEPNSLTDTNTKFIVPIKLSDKAMKLSDDVKNELKSIGMMDEK
                     GKLKLKGVSPKMLKRMKMESVDCPVLQREISFIPCYVCKNFHSRISGQVYCRGEPL"
     gene            4874..6322
                     /gene="proS"
                     /locus_tag="VF222_00995"
     CDS             4874..6322
                     /gene="proS"
                     /locus_tag="VF222_00995"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7141032.1"
                     /translation="MSELKEIGITTRKEVDFSEWYTQVVTKAGLIDYSPVKGFIVLKP
                     YGYKIWEIIKDSLDKKLKESGHENGFLPILIPESLLSKEKEHFEGFTPEVFWVTKAGS
                     TDLLEKFAVRPTSETLAYHIFSKWIMSYRDLPLKINFWNSALRAEIKSTKPFIRNSEF
                     LWQEGHTVHENQEEAEEEVLLILNYYQYIIENFLAIPTVQGIKTEREKFVGALYTNAL
                     ESLMPDGKALQMATSHNLGQNFSKPFEIRFLGRDSKQYFAWQTSWGISWRVIGALIMI
                     HGDNKGLILPPKIAPTQLVIVPIFKNKERAIVIEESKKLFVELKNLDFRVIVDDREEY
                     TAGWKFNDWEVKGVPLRINIGIRDINNNNLEIVRRDTGEKILIKRVDCIKSVHFYLDQ
                     IQKSMFEKAKNIQDNNIIKISNYEEMKKILNEKGGFILADWCEKEECENSIKNETGAD
                     IRLIPLEKYSSDNKCIYCNENSKKTVIFAKAY"
     gene            6441..7118
                     /locus_tag="VF222_01000"
     CDS             6441..7118
                     /locus_tag="VF222_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7141033.1"
                     /translation="MDGINDILTFFDFGYEEYGNVGRYFGILIITFFASIIVFIPVPY
                     FPVLFTAGINQNLDPHLVSLFSAVGAVMAKLIIFYVSYYGRNILSTKTKKRMLPLQKL
                     LSKYGWPGAFAAALTPIPDDLVYIPLGLAKYNPAKFAIATFSGKFILNEFIVFGSVFL
                     GRPVVEVLTSDNTDPIYLYIGVGVSISILILIIFLYLRIDWGKIIGKWFPWTLNNDDM
                     DNRSNTK"
     gene            7169..8122
                     /gene="hemB"
                     /locus_tag="VF222_01005"
     CDS             7169..8122
                     /gene="hemB"
                     /locus_tag="VF222_01005"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7141034.1"
                     /translation="MVEHEDNYYEFSKRNSLIKNDLIFPLFVDENNKFQELNVMPGLF
                     RIPYNNISQALEEIVENGIHSIILFGIPRFRNTIGTSATKKNGIVQKTLMLIKSNFGN
                     KITTISDVCLCQYNNTGHCGLLEKAKKVVDNDLTNERLSDIALSHAISGVDIVAPSSM
                     MDGQVKRIREKLDANGYNKIKILSFSAKQSSSLYKPFRSETFFPSSNEIDKSTYQVPY
                     YNSREIQREIEMDIHEGANMVMIKPAMSSLDLIYRIKKNCTLPLVVQVVSGEYSIMKA
                     AATIGLFDETSYVLNLLSALKRSGADKIISYSSLNISKYLL"
     gene            8188..8348
                     /locus_tag="VF222_01010"
     tRNA            join(8188..8225,8282..8302,8334..8348)
                     /locus_tag="VF222_01010"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8222..8224,aa:Tyr,seq:gta)
     gene            complement(8357..9409)
                     /locus_tag="VF222_01015"
     CDS             complement(8357..9409)
                     /locus_tag="VF222_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX7141035.1"
                     /translation="MISARIVKTNEPLIIEKVPIPKPKNSEVLVRVKAAGICHSDLHL
                     WEGGYMGAGGKFMRVEDRGVKFPLTPGHEISGVIEDIGESVLHFHTGDNVLVYPWIGD
                     EICHSCKAGDEHLCESPKTLGIYQDGGYSDYILVPNYKHLIKLEEIDFNSSAALACSG
                     LTAYTSIKKSLGMPGENLLIIGAGGLGLMAVQIAKAITNSNIIILDVDDEKLKEAKKL
                     GADMTINSLSSDPVKSVKDFTNGKGCEVLVDFVNNSKTASTAIEVLRKKGRYIMVGLF
                     GGSLELNLPLVPLRAFTITGAYTGRFNDLVELVSLFKKGKISSVTTRQYKLEEANQAL
                     EDLKNRRIIGRAVFNP"
     gene            9729..10001
                     /locus_tag="VF222_01020"
     CDS             9729..10001
                     /locus_tag="VF222_01020"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141036.1"
                     /translation="MKNRADIEVIASILRSAKDKWEYQTTIMNKASVSHSQVIRYISV
                     AMNTHLIEYSKITGLYKTTKMGLDFLEKHGKLIILFPEIAEPSQCS"
     gene            10073..10759
                     /locus_tag="VF222_01025"
     CDS             10073..10759
                     /locus_tag="VF222_01025"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEX7141037.1"
                     /translation="MRVACLFSGGKDSTYSLYLAKQWGLSVDCLLTLIPHSVESYLFH
                     SPNIWTTQLQSQALQIPIIMKQINSISIDDECDKLDELINQANKDYKIDGIVHGGISS
                     NFQKNKFNKICERYNLKLISPLWQIDQYDYMNKLLKNNFVIQIISVSSMGLDVNSLGI
                     ILNHSTLQDLFKLSRKYGFNISFEGGEAETIVLDCPIFRKRLEILTSKIQWDGQRGIV
                     EISDIVLLDK"
     gene            10760..12127
                     /locus_tag="VF222_01030"
     CDS             10760..12127
                     /locus_tag="VF222_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX7141038.1"
                     /translation="MLDNLRTGLRSALKKIVGASDINEELIDSLSKDIQRSLLQSDVN
                     VRQVLSITKNLKERALKEQPPKGLTKKDHIITILYNEISSLLGYKGTQILHIDKAQSI
                     PEDSFNFQPGKKNIILMFGIQGSGKTTCTVKIARWLSRHGYKVAIIGADTWRPGALTQ
                     LKMNSIKIKVEVYGEEENKDPISIVKNGLEYYKSQDYDVIVIDTAGRHKEESGLLEEM
                     NEIHKVTNPDLVLLVIDGTIGQQAYSQAKAFHDAAPIGGIVITKLDGTAKGGGALAAS
                     AVTGAQISFIGTGERIDDLELFSPTRFVGRLLGMGDIKALLDMAKSLEIEADETQSKR
                     LLGGKMTIEDFYSQMENVNKMGFRNVIENLPGLSGMVKDEQLDVLQGKIEKWRFIIQS
                     MTKVEKKDPNLINDARRKRISRGSGITEHEVKDMIKQYNNSKTMMKQAKGRQMHGMLR
                     RFGFG"
     gene            12314..13246
                     /locus_tag="VF222_01035"
     CDS             12314..13246
                     /locus_tag="VF222_01035"
                     /inference="COORDINATES: protein motif:HMM:NF044739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141039.1"
                     /translation="MIKFQENYSYRSFTIGSSLPYFMFDKEDYIRSRYKLKDREDIKS
                     SFNFEIRNKFKTLSKKKLNLINPDIKINIFFSGNNEYSITILSRSLYLLGRYNKKKFM
                     AQREKNSLKNDPNGNHDEIISKKQTIETIIKKTLSNCIRSDRMVFTWYGSEDDNSQVL
                     GKGRLFLIQLINPRERTIKINKVFTDDGMSFKIIEKRDFFGNIKRNFRIKNKIFIKAS
                     NPIDKIHLIKLMNLNNSIISYRYKSKTIHKKIYYIKFKKLDPLHLIINIECDSGLFLR
                     QFVEGRNLIEPNISLVLHTQCECLKFDIMDISVS"
     gene            complement(13951..14136)
                     /locus_tag="VF222_01040"
     CDS             complement(13951..14136)
                     /locus_tag="VF222_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HEX7141040.1"
                     /translation="MTDKKNEKDSQVYKFYKLDGNSVERIKRDCPRCGKGVFMAAHKD
                     RLTCGKCGFTEFIKKKL"
     gene            complement(14151..14471)
                     /locus_tag="VF222_01045"
     CDS             complement(14151..14471)
                     /locus_tag="VF222_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141041.1"
                     /translation="MLNRRELKIFLKSAAGKISKIELVKMISKNFNEDEKKIFPISLA
                     GEKGKTDLTATLFIYENGEIAKKHLPKYRILRKLSKEDRKKIIDEEKAVKLKAKQAAK
                     ADTK"
     gene            complement(14870..15946)
                     /gene="trpD"
                     /locus_tag="VF222_01050"
     CDS             complement(14870..15946)
                     /gene="trpD"
                     /locus_tag="VF222_01050"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7141042.1"
                     /translation="MTNIELNIQKILNKFSSEENLSFKEAKVIFKNIVEGRMQDSYAS
                     SILKEISLKGETVDEILAMVQIIKESSLTIHPKFKNNLFDICGTGGSKIKTFNISTAS
                     AIVAASAGCNIAKHGNRSASGFCGSADFLEYIGLNLGNSPHIVSRSIEELGIGFLFAP
                     LFHPVLKNVGKLRKEIGMKTIFNIAAPLCNPCDNLSGQIIGVYDAALLDKFSKVTKKL
                     NKKYILMNSIDGIDEISNTCLSKIIYVNKGEIHRFNLNPQEVGIPLTSLNNITIKTKK
                     DSIDLTLKSIYGLARKEIEDILAMNSALALVLSEIAKDIKEGIDISRSVIREGKARKK
                     LKDLITFCGNKDRLLEIEKDLDLS"
     gene            complement(15943..16530)
                     /locus_tag="VF222_01055"
     CDS             complement(15943..16530)
                     /locus_tag="VF222_01055"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7141043.1"
                     /translation="MNILIIDNYDSFVYNIAQSLGRSNITTYVHRNDTITLNKIKKMN
                     IDAIIISPGPGNPEDKKYFGICNNIIKILGKEIPLLGICLGHQGIVSAFGGKINNAVT
                     TRHGKTSEIYYKDDPLFEDVKNPFVATRYHSLIADKETFPNCLDITANSLDDNEIMAI
                     THKKYPIKGVQFHPESILTNEGLKILNNFILFIKK"
     gene            16640..17692
                     /locus_tag="VF222_01060"
     CDS             16640..17692
                     /locus_tag="VF222_01060"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7141044.1"
                     /translation="MVEMVKLFINGYGNIGRRLATAFNLDEEVDLVGIAKYKADERAK
                     EAISKGYKVFIPPSLEQEFKNNKLEFTGFIEEAIQKSDIVIDASKEGLGFDNKQNFYL
                     PMKKMAIFQGGEDREGKHSVADIIHNSRVNYEKMVGKSYVIQGSCNVSGMGRVIQPLI
                     DQFGDRISRYDVTLIRRWADLEDLSEVKDSIQWDKNPHHQDDVKNFIPNIELFVDAYK
                     VPSRMMHLHQMYIRFKDKAPTKNEVLEIFSNEFGVAILNSAKGTADIRKKALEMGFIH
                     GDTNMIHIHQEIMRSQGDVLKISYSDDQTGMVIPENHLLVQSLFFKRSRKKALSHTDK
                     IFELSKKKKVLQEEFK"
     gene            complement(17892..18146)
                     /locus_tag="VF222_01065"
     CDS             complement(17892..18146)
                     /locus_tag="VF222_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141045.1"
                     /translation="MTMPKGFGSNVSQNGKRNFNDWWNSVPADLKQKARKGDENNKPL
                     LNQVNYVLLHLHLAGKHDAKPSHDELKDWLHSGQVDVLRK"
     gene            18314..19363
                     /locus_tag="VF222_01070"
     CDS             18314..19363
                     /locus_tag="VF222_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HEX7141046.1"
                     /translation="MTFKSITLGEMRKDYVMDKFVIYPNNLEEIKNDNSSLTICPYCP
                     GNENNTPSAIVSLVIKSGILQRLSDSEGNIVKDWSVRVFNSNTPIIVQNNSTLYTDKP
                     LYSEPAYGYHQVLVATPDHSDSFSKISVEQWCNVLLVLQDRIRWLYSQKKVTYVSVYI
                     NNGNRCGSKIDHSHIDIVSFSEVPPFVESKALSSHRYVNENGNCPSCIAISTELDGPR
                     QILATDYFIAFCPWASIYPYEFAIYPKKHSTTVTKISQKEIFDLAMILRSTLGGMAQV
                     LNNPSFNLIFQLSPEKKNSRQFHWHIEIFPHISELTGLEKGFGVFVNNVLPETAASKL
                     GAAARKEIAKIVGVE"
     gene            19364..19825
                     /locus_tag="VF222_01075"
     CDS             19364..19825
                     /locus_tag="VF222_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141047.1"
                     /translation="MILKNSLDLESWLSIASLGLAIMFVLLIVSFYNVLISNEGQGPE
                     RFVDIRGVVVKTLSISGAPSIILAAISFGLSKNYGNRLIGMLLAASGIVLIIGMVISL
                     SISSNINSEFFHPILVLVPYTFIVGGIAIFIIGVIVYLKTKKGIKKTGRFN"
     gene            19822..21285
                     /locus_tag="VF222_01080"
     CDS             19822..21285
                     /locus_tag="VF222_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX7141048.1"
                     /translation="MKTTNIEKIEISRQNLPINVNSTLLRDIFNLAKLENSPILSVVI
                     GTKPDFYKQAPLVVEAKREKLPVFVIDTGQHFDEILGFGIKEFKLEESVGCDLQIRGD
                     LMEKASELILKFGSFGRFCKEEFGSTSKLLPVVHGDTLVAGIAPLAWVFGMGQKVAQN
                     EAGLRSMSPEIMKKLKITQEPTLDLIEKYIQEQFDGKWFVAREEPFPEQIDTWICSAG
                     TQYFFAPTQLNKDNLVKEGYPDEFVHVVGNSVVDAIYLKRKQKVSQSIFDLYPELEKG
                     KWIRMDIHRRENLTEHRFKSIIHGLVNIVKYSDHKVVLVMLNATKSALKFYGLEEKVR
                     LLADEFPHKFLITPLWKEYGQVIEFLDSGRCWAEMTDSGSMQEELLYFKNVLSLTIRL
                     NTDRPETIFDAKSNLLIPPINSEWITSMVNHLYDKFEDFGVKSKNKKEIYGEPGQVSK
                     TIAKIIKKQFEDKNTSFYPWFHQRINLWKEDNDLQYM"
     gene            21525..22685
                     /locus_tag="VF222_01085"
     CDS             21525..22685
                     /locus_tag="VF222_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HEX7141049.1"
                     /translation="MSNGNNGENLLGRDLISRQNSFNCIIVLCIDRDDDLGSKGGIDT
                     PVIGRDSCINAGIRLAIEDPEDSDANAIFAAVKTYEDLLKRGYNVEVALVAGRYNRGI
                     EGDEKIGSEIKYIVDKFKADASVIVSDGEDDETVVPVIQSMVPIISVQRVVIKHSRSV
                     EYSYAIFAKYIKMLVYDPRYSKFFLGVPGTLLVASGIATIFGLTREAIALVLSILGSA
                     FIIRAFDIDKSISALGKPTPTSFIRIFSLVSGLLIIVVSIVNGLSHIPEGIVREDMTN
                     FEIVTNKEIVGSFLRGTILLLWIGIGTILSGTLLSNWFRGSIKTMSDILRLVILILLY
                     YPILQFTSVLTEGTSPFNLISSLLIGLAITLVAATFLYQYYRNRKGGESLKN"
     gene            22830..23558
                     /gene="uppS"
                     /locus_tag="VF222_01090"
     CDS             22830..23558
                     /gene="uppS"
                     /locus_tag="VF222_01090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7141050.1"
                     /translation="MESQISRDPLPKHVAIILDGNRRWAKYHFFGIDKAHFIGAEKAE
                     ELLNWIYDLEIKITTVYVLSDENLERENEELTNIYNLLSKKLTKLKDDNRIHKRKMKI
                     KAIGNIELLPKTLQQILKILEEETSSYNSMFLNIAVAYGGQQELVDAIKKIASMVTSG
                     KLKVNDINKKIIESCLYTSHLPQSDPDLILRTSGEKRLSGFLLWQSAYSELVFMDVFW
                     PEFRKIDFMRAIRTYQNRIRRFGK"
     gene            23548..23763
                     /locus_tag="VF222_01095"
     CDS             23548..23763
                     /locus_tag="VF222_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141051.1"
                     /translation="MENKVTTKIMYSFSIYQMYEHGYYDSHLKKWHCGYFMDEEEWLD
                     IHDYLPNQFKEYFSNSESSEEENDKIA"
     gene            23782..24213
                     /locus_tag="VF222_01100"
     CDS             23782..24213
                     /locus_tag="VF222_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7141052.1"
                     /translation="MTSEISCGALVYCIENHKIIFLLLHYFHGHWDFPKGNKERGENH
                     METTRREIREETGIGDITFIDGFEKQISYKYKRQSELISKKVIYLLARTISKDVVLSS
                     EHTAFVWEPYENALKKLTYKHSKEILTEGYCFIKNFINSAT"
     gene            24276..24632
                     /locus_tag="VF222_01105"
     CDS             24276..24632
                     /locus_tag="VF222_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7141053.1"
                     /translation="MFLNDKHYHIFKNELHLSELEIKIFLLIISQGRLKQQQISLELN
                     LDKSNCREIIESLINKNMIIEYSNNLFECFHPRFAIINRYKRLCVEKDIPWKKNSVID
                     NLAIMLEQSFEAARTK"
     gene            24657..25070
                     /locus_tag="VF222_01110"
     CDS             24657..25070
                     /locus_tag="VF222_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141054.1"
                     /translation="MSENDFKTNDKIVYFGVININEEGKTIGAVDIWRSVITKEIFCE
                     EKRLGILDIADYIGMPRIEKGNKWAVAVNRNRFGKDRWKLIKILEEGTFKFYDTDDET
                     IINTNVQNFHIIDNDWWNFLVESNINRSVEITKEL"
     gene            25074..25370
                     /locus_tag="VF222_01115"
     CDS             25074..25370
                     /locus_tag="VF222_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141055.1"
                     /translation="MYGFGDNEDLKIDVFINNSSGGEVASKLTGYFVVKNKTFRFSAI
                     AFGRIGGHNISLKLSNASINKLKKMEIDPEKLQLTIQRKLIEGDVNLPANMKLP"
     gene            complement(25475..26284)
                     /locus_tag="VF222_01120"
     CDS             complement(25475..26284)
                     /locus_tag="VF222_01120"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX7141056.1"
                     /translation="MIKNLKYRSTIGVIGGTGIYDSNIFNIIDEIKINTPYGETSDRI
                     TICTYNEKSIAFIPRHGKNHRIPPHKVNYQANIWAMNELGVKVILAPSAVGSLKSQYR
                     PGDIILPDQYIDFTKKRRYTYYDGPVVCHISQSDPFCPDLRENIVRDLKQLGFKFHPV
                     GTYICIEGPRFSTKAESNFYRTVLKADIIGMTLIPECILAREMEMCYVSISTVTDYDV
                     WAEVSVNSQEIIETLSKNVDKTKKMLLTIIPKIKEDRNLCNCSTSLENALI"
     gene            complement(26277..26819)
                     /locus_tag="VF222_01125"
     CDS             complement(26277..26819)
                     /locus_tag="VF222_01125"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7141057.1"
                     /translation="MLYNHKINIKSLIKNFNDFPKEGVIFRDINPLFRNSIAIQAIAK
                     EFIKKEELSKIDLIAGIESRGFTVASLLAKNFNKGMILVRKVGKLPGKTIKKKYDIEY
                     GSSVIEIQEDAIQKDQNILIADDLIASGGTASATAELIEEMGGRVFGFAVIIELVYLK
                     GAEMLRKKGYDVRSLVKYYD"
     gene            complement(27127..27609)
                     /locus_tag="VF222_01130"
     CDS             complement(27127..27609)
                     /locus_tag="VF222_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7141058.1"
                     /translation="MKNLNDEGIELLDAEKINEFNTLRIHNLTFKPNLDKQDFSGKNL
                     SLAFLNGALCNKTNFSNCRLKKTNLVQAELNNSNFQNSNLTDALFMYAEMNECLLTNC
                     NMKRSNFMWANLQNSDLSNSNLANTIFIEANLRHSILHNLNKKNAYINLAKLEFTEWK
                     "
     gene            27750..28160
                     /locus_tag="VF222_01135"
     CDS             27750..28160
                     /locus_tag="VF222_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141059.1"
                     /translation="MNFSKTTHIPPRIMVVDDERDILSIIKRGLESKNRFQVETFIDA
                     ESALESLKENPDDYYDLVLTDIRMPKINGFELYRRIKESNPHMKIVFITAFEINKEEF
                     SKVIPSLDVIDFISKPVSMSTLMTKLNSILNQIN"
     gene            complement(28203..28664)
                     /locus_tag="VF222_01140"
     CDS             complement(28203..28664)
                     /locus_tag="VF222_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141060.1"
                     /translation="MRLRYWHLTISEILNAKYPTDKVNHWEIKCLYGEHEHFGIFWYK
                     YGTPFDKEPVHGICFYYNEIPEKIVRELTDYLVSTFGGKILRRQSRVFLDGSKEIHDP
                     YNIGQLANEISLKFSIPVEITLEFEKVTKEEVDTNSFNFPSKKALPIVGID"
     gene            28745..29242
                     /locus_tag="VF222_01145"
     CDS             28745..29242
                     /locus_tag="VF222_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7141061.1"
                     /translation="MKINYNENYDRLKLIGEMEKEFNNKNIQFFSKLAFHHDPVIRTR
                     SICIIADIGEEKGVDTISKALKEDVDDLVRHEAAFSLGQLGFKRGIKPLSYAVENDPS
                     IFVRHEAAVALGVIGSEAARSVLVTALSDPSEEVKKSAIVALANLDYILNSKRINKFT
                     RMTGG"
     gene            complement(29262..30311)
                     /locus_tag="VF222_01150"
     CDS             complement(29262..30311)
                     /locus_tag="VF222_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HEX7141062.1"
                     /translation="MSNAIDTRDPIISTLGSHCSLQVLKGAKDEGFKTLLICEKKRAS
                     LYKRYKFIDDIFFLDNFGDLLSKTVQDELIDKKIILIPHGTLISNVKFEELEKINIPV
                     FGNKWILRWEADRVLKHKLMNKSNFNTPNEINRKNEIKNLCIVKLHGAAGGKGYFLAW
                     NEHSFDNGCKKLINQKVIKSEKELYIQEYVLGVPIFLQFFYSPLTDEVELLGIDRRYE
                     SDIDGIGRIPAHIQIQLDLDPSYNVIGNFPLVLRESLLQEVFTMAENFAKKSKELIPP
                     GIPGPFCLEGVYDKNGKFVIFEFSARIVAGTNLFINGSPYSDLLYNEPMSMGRRISRE
                     IKEAIKSNKVHEIIT"
     gene            30460..31833
                     /locus_tag="VF222_01155"
     CDS             30460..31833
                     /locus_tag="VF222_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7141063.1"
                     /translation="MNEDILFDNNISQLLLSLDDKIRSGEYKIAIYGLGHVGSPIAAA
                     WLRSGAHVIGVDKSKSILQYAREGKTHIPEPGVNEAFEKGINNQKLSIYDDLVKASKD
                     SFLKMICVPVLAADGTADLSAVKNVAVAIGRGLKKNDVVAINPSVPPGTTEEVVIPIL
                     QKNSGLTVEKDFYIVYNPERIYEGRAIEDIEQRYPGIVAGSGKKSLEISTKIFSFIYQ
                     KGIISMSNIKAAEAEKLFEGVYRDVNIALANELAIFSERLGINFWEVRNAANSQPFCH
                     IHKPGIGVGGACIPVYPQFILKTAKVLQVDCKLTRSSRLLNNSMPKYCVRQALKLIKP
                     KKYKNLTITLLGLAFRGGVSDTRLSPTYEVIKELQKLKVKEIRVHDPLVKKDDLLSRY
                     RNTFLINDLDLAVKGADLIMVIADHNEYKKLNSTSIGKSALYDGRGITMEDKLHGVRH
                     AMIGNLN"
     gene            31889..32935
                     /gene="mtnA"
                     /locus_tag="VF222_01160"
     CDS             31889..32935
                     /gene="mtnA"
                     /locus_tag="VF222_01160"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7141064.1"
                     /translation="MTKLSNKLLLTLEWKNNYLVLIDQTKLPTKLTYVRCRSYKDVAD
                     AIRKLVVRGAPAIGVAAAFGLALAAINSKTNNLKIFSKELSNAYATIRSTRPTAVNLQ
                     WALDRIMQKIMNLKDIPHIKKVILREALLMAKEDIETNKSMGINGANLFSDGDTIMTH
                     CNAGSLATVAYGTALGVIRAVKESGKKISVIATETRPVMQGSRLTAFELQHENIDVSL
                     IPDTAVGHIMSSGIVKHVIVGADRILSTGHVFNKIGTYQLAVLAKTHNIPFYVVAPAS
                     TFDLKNKPKDIVIEERSKDEVIRIGKKIVAPPNIGVINPAFDMTPPKLITKIITEKGI
                     VSPPYTKSIKKIIG"
     gene            33014..33613
                     /locus_tag="VF222_01165"
     CDS             33014..33613
                     /locus_tag="VF222_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX7141065.1"
                     /translation="MSKESITKDQVYVQLKKCMDPEIPVNVVDLGLIYNVEIEAEKDV
                     NIKMTMTTRGCPLHNTMVSDVKKYVGKINGIGTINVEIVWDPPWSIDKMNPNARESLG
                     FGKPKLRFQINYEKALPKKVGSYMKSEDGSLILVNKDESSFMVNDAIVRFWESCDGTK
                     TINQLTEYFSEKLGIPIQQVEQEIVQLLEQLFDAKLIEV"
     gene            complement(33648..34061)
                     /locus_tag="VF222_01170"
     CDS             complement(33648..34061)
                     /locus_tag="VF222_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141066.1"
                     /translation="METKKRKKIKSDNKPEKEVIPSEKWLVSFKNNNNTKGVIPRLII
                     RQFYAEGYYEAYDIIRTYAEKLALEIMWFKEKRNCSNMFISKKFPLLESICIYCNRIF
                     NDLEPIPCLKENCIHTFCSTDCLKNHIRLRHIATK"
     gene            complement(34076..35590)
                     /locus_tag="VF222_01175"
     CDS             complement(34076..35590)
                     /locus_tag="VF222_01175"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7141067.1"
                     /translation="MEQYQERYRALFSWIIFMVIYDRIAKLSLERGFYFPSSEIYSDS
                     SAGFWEYGPNGNNIKNKFIELWRRELVRRDDMVEIDGSQIMPKNVFVASGHIDNFSDP
                     VIVCMKCKSTFRADKFILDRAGINVPERVPNHEVDKILNQHNLKCPKCGSSFQASERF
                     NMMFKIGIGPNKDEAYLRPETCQTIFVDFPRIFKIMRGKLPFPIAQVGKSFRNEIAPR
                     QSLLRLREFYQAEIEVFCNPNQLDALSKFDEIKNTTLTIDDGVRVSKVTAQEALDSKI
                     IPNKLVAYYLSILYEFYEKTGIDMNQTRFRKLSDQERAFYATVAFDFELKTSVGWLEL
                     VACNYRSNYDLSQHSQISKEKMEIMDPVTNEKVLPHIFELSLGIDRSIYSILEHCFYE
                     DHENNRVILKLKPYLAPILAAILPLVKKSELVEISKNIYSNLKKDFDIYYDDSGSIGR
                     RYRRLEEIGVPFAVTIDYDTLNDNTVTIRYRDTMEQRRIHINDIKSFFLQNKNP"
     gene            complement(35590..36456)
                     /locus_tag="VF222_01180"
     CDS             complement(35590..36456)
                     /locus_tag="VF222_01180"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX7141068.1"
                     /translation="MGFHVSITGSISNSIKNAESIGCTAFQIFTRNPRGWRAKSLEMN
                     DVKEFKSKINDSYIDKNDVVVHIPYLPNFAAPDGESYNKSKDVLAEEIIRCYELDIPF
                     LILHLGSHLGFGEKNGINQIVNACNFALDNFHSSYRRHLRLNILIENSSGQKNSIGSK
                     FEEIRDILDVLNDRKFGVCFDTCHAFASGYDLRTAEKVIDTMNSFNDIINIKNLKVLH
                     LNDSKGKINEKKDRHQHIGLGSIGKEGLMEVLKNKHFQNIPIIMETPNDGIQGDKENM
                     KVVSKFIENHIM"
     gene            36548..>37323
                     /locus_tag="VF222_01185"
     CDS             36548..>37323
                     /locus_tag="VF222_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141069.1"
                     /translation="MSIILGKDDLAKYPFLEEAGNYIRETHFDLDDFNRSEFLHIIER
                     ATKRIEFELIQGKVFIDLERYDIELFTFLVSLIIMKCINEDSAIKKFSLFEAMRVEKF
                     LISDLLKERDLTKKNLLISKIFRELFHLDVEIDPNNNNVFKMNISDYLQRATSFNEEE
                     WKLIGRAVDNGYVYLDGDETVRLIRYEIYKLMYNRIKNMNITKIPDQIKLHANLIKNK
                     FPQPVKYTATITDLPPCIKKALELLNKNENLPHSARFMLAT"
BASE COUNT        12877 a         5552 c         5957 g        12937 t
ORIGIN      
        1 tcaatgccgg aggcgggatt cgagcccgcg acctttcgat tatgagtcga acgctctaac
       61 caagctgagc cactccggca agctcagcag cgtttcattc ctgttatttc aattgttata
      121 tatattctat taatattcta attcccccag aatttgtgaa attaacaagg gataagaaat
      181 accccttttt agtgtatttt tgtttgtaaa cctgttataa aaaagaatta agtattgtat
      241 gaatttcctt atctcttgga taattcaacc catttaaatt ttctttgtaa agtagtagta
      301 taaatttttt tccaatctaa tgctacgtaa tctaaccaag ttttcataat tctgggatca
      361 atataattcc ttaaagatgt acctaaatta taatcacgtg tttctttttg taattttaat
      421 tggtactgaa ttttttccat tctatatttt agtctttctt tctgtttttc tgttgtccat
      481 ttattagaca atatatcttt ctttattttc cctatggctt cttccttttt ggtaatcgat
      541 tctgcaaatc tgtctaatgc ctttttactt gcaggattgt ttggatcaat acctttctta
      601 tgattacaag caatcgctgc ttttagattt gcaattttgg ccacataaac cttttcaggt
      661 tctgatgatt ctacattcac atcaagaggt ggagcattta atgcttcttt tactattttt
      721 gttgcaagat aagttctaaa tacctttgca gtaagaccgg ggaccttttt tgtatctatt
      781 tttcttaaaa atgcattaac ctttgaggaa ttaatattat caaaaatttc attattgtct
      841 tgtttgtctc tcataaataa tcgaagatta tcgtataaac cttttgtgtc tactgaattt
      901 acttctaatg ttttttgcca tggaacacta tcttttccca gaaaattaaa ttctataaat
      961 ttcttattgt ctttttcagg aaattggatg tgttctattc gcaatgtcga tgcacctaca
     1021 gtatctgcct cgtctttatc tttttcatct cctactctca ttgctaattt aaggatcaaa
     1081 taacaaactg ttgctatttt acgctgattt aaatccttag aattaatcat tttgtttatg
     1141 atttcttttt cgaccgtgtt tgcatacttt tcgaggtttc tagctttgtc atatttttct
     1201 ttatcattat tttgtcttaa aaatgctgaa tcatgtaacc aaacatattt tctcttttca
     1261 gttaattttt ctgtccaagt tgcgagccac gttgatgaat gatcatgaac aatacttttc
     1321 catgcccctt cgggaacagg tgcatcttct cccaaattca aaataacatc ttgaggtatc
     1381 actcttggtt tccatcttcc tctaagtgga tgcaatcctc ttcccatgaa taaaccagga
     1441 ggctctacta accagtttgc aacttccact tctaatccat caacataagc ctttccataa
     1501 aaatttttta gtctttctct ttcctgtttt tttgtttcta gcaatttcct cttttcttct
     1561 ctacttaact ttaggattgt ctctttttct ctaattttta atttaatttc ctcatcaaca
     1621 agggtaaatg cttctgagaa atcaatttca tttatgttat ttgtattgtg gaatcttggt
     1681 ggtaatattt ttttaaaatc atttaagaaa ttattttgga aaattacatc attaatgtaa
     1741 tgagtatctt tcttttttgc ccatgcatag actatttctt cttgttcgtt attaagtgct
     1801 agtcgttcat tgtttatctt tatacttact ccttttgaaa tgtgattagg aggaaaagct
     1861 actccattat gctccaatgt tttccattta aaagactttt ccacggtatt tgacattaat
     1921 agacacagat ttaatggtca tgttttacta ttgataaatc ttaccaataa aataatccta
     1981 aattcctgtt aataaaaata caggaaagga tcgaaatcaa ttttttttga atctaaagtt
     2041 atccaatttt attaattctt gatatgatcc aaaatccagt ggatcagatt tatcaagcaa
     2101 ttcatcgtca aagtaattat cacttgaaaa tgttttgata atttgataat ttgactttct
     2161 ttgagcaggt attttgccaa tattatgtat taaatttctc atcactttag gttttaatag
     2221 ctggccaaaa gtcgctcctg cagaggttga aatgctttca ttgatcaatg tcccaccaaa
     2281 atcgttagcc cctgcttcta ataaaatctg tcccattctg ggtccctctt ttacccatga
     2341 aacttgaata ttgtctatat agttattgaa ataaattctt gcaattgcat gcatttttaa
     2401 tacttcaatt ccagttggtc catttttaat accgtcaata agatttctat tatacattgg
     2461 tgcttcagta tgaacaaaac tcaatggcac aaattctgta aatcctccag tgtctttttg
     2521 aatatctttc agtatactca tatgtttgat aatatgtgta taagtttcta tgtggccgta
     2581 cataatagta gaagtagaat taattccaat cgaatgtgca catttaataa cctttatcca
     2641 gtctttgaca cttattcttc ctggagaaat tttatctctg atttcttgat ctaatatttc
     2701 cgctgcagtt ccaggaatac tccctaaacc tgcttcttta agcgttttta aataatcttc
     2761 tattgaataa ttagctctgt tcgcgccgta aaggatttcc tcaggtgaaa aagcatgaat
     2821 atggatattc ggcaactctc ttttaatagc attgcagata tccacatata acatgccgtc
     2881 cattctcgga gggagaccag cttgaataca tacttcagtc gcgcctattt tttcagcctc
     2941 ttttgctctt cttactattt cttcaatagg taaataatac ccttcttctt ctctaaaatc
     3001 cctactaaaa gcacaaaaac cacatctttt gatgcaaaca tttgtaaaat ttatatttcg
     3061 atttatgaca tatgttacaa ggtctccttt ttttctttta caaagatgat tagcaacaat
     3121 tacaatcata ttcaattcca atccactaga attgaagagt tctatagttt cctcctcaga
     3181 tatgttattg ccatcaagta ctttatttaa tatagtcgaa ataatgggat ctatattttt
     3241 tattatgcta tctaaattca actaaatatt cctctttcac taatctatct aaaccaataa
     3301 agttttctac ataatttctt atctgaggag atataaagtc aggtttgtcg atatactcag
     3361 gatatacggg taatctagcc cggagccttt gataccttga ttcagtaatg ttttttaact
     3421 cttttaaatt tggccaggga aattcaggat ttacatgatc gattgttact ggtgaaattc
     3481 ctccccaatc attaatccct gcatctaaga aagaactaaa acttgaggga gatagattcg
     3541 gaggaatttg aatattcata tcaggcatta ttaaacgtgc aatagatact atctttaaaa
     3601 aataatcgtt aggtggagaa ggaaaattat gcatccgtgt atttgttttt ggctgaaaat
     3661 tctgtaaaat tatctcttga atgttctcaa attcattatt gatcgttttt aaaagaaaca
     3721 aatcattaat tatttcttct cgagtctggc cgattcctat caataatccg gtggttaagg
     3781 gaattttcat tcttccagcg ttcttaatca cctcgatcct attactggga tttttactag
     3841 atgcaaattc atgaggcatg cctttttcaa tcaatttttc acttgaagat tcgagcatca
     3901 agcctagact tacattatat ctctttaata agttcatttc atcttttgta atattcccaa
     3961 gattagaatg aggtaataag cctgtatctt ttacaatttt ttcagatata tcaattacat
     4021 attctactac tgaattatat ccaagatgtc ttagccattc ttttgcttcc ttgtatctat
     4081 cttctggtct ttccccagct acaatcaaag cttcagtaca gtgaaatttt ttcccaattt
     4141 ttgctagatt gattacctcg gtaggtttca tcataattga agatgattca aatggatttt
     4201 ttctatatgt acaatatgaa caaaaatctc tacatagatt agttatatta ataaagattt
     4261 ttcttgaata agtaataacg tcttttttta gccttgtttt taagaaggtt gatagtttca
     4321 aaatagatat aatgtcatca gagtccagaa ggcgaagggc atcttcttta gaaatactct
     4381 ttttttcttg acatttttcg agaatttcag atatagactt gtctttaata gaaattggaa
     4441 tttctgaatt catcactatt atatccatta gtaaagtaat ttatctttaa ttttttttag
     4501 ggtataggaa acatcatata ttctatcatt agaaaataaa gatatgagtg aacctaattc
     4561 tttaactgat accaatacaa aatttatcgt acctatcaag ttatcagaca aagcgatgaa
     4621 acttagtgat gatgtcaaaa atgaattaaa aagtattgga atgatggatg aaaaaggaaa
     4681 actaaaatta aaaggcgtta gtcctaaaat gcttaaaaga atgaaaatgg agtctgtgga
     4741 ttgtcctgtt cttcaaagag aaatatcatt tatcccatgt tacgtctgca aaaattttca
     4801 tagtagaata tcgggacaag tatattgtcg tggtgagcca ttataacaaa tacatttaga
     4861 tcaagggatt tttttgtcag aattaaaaga gataggaata acaactagaa aagaagttga
     4921 ttttagtgaa tggtatactc aagtagtgac caaagccggt ttaatcgatt attcaccagt
     4981 taaaggattt attgtactca aaccatatgg gtataaaatt tgggaaatta taaaggactc
     5041 tctagataaa aaattaaaag aatcaggaca tgaaaatggg ttcttaccta ttcttatacc
     5101 agaaagtctc ttatctaaag agaaggaaca tttcgaagga tttactccag aggtattttg
     5161 ggtcactaaa gcaggatcta cagatttact tgaaaagttt gctgtaagac ccacttccga
     5221 gactctcgcc tatcacattt tttctaaatg gattatgagc tatagggatc ttcctttgaa
     5281 aatcaatttt tggaattccg ccttacgtgc tgaaatcaaa tctactaaac cttttattag
     5341 aaattcagaa tttctatggc aggaaggcca tactgtccat gaaaatcaag aagaagctga
     5401 agaagaggtt ttgctgatcc ttaattatta ccaatatatt atagaaaact ttttagctat
     5461 tccaaccgtt caagggatca aaacggagag agaaaaattt gttggagccc tctatactaa
     5521 tgctctcgaa tcgttgatgc ccgatggaaa agcattacaa atggctactt ctcataatct
     5581 aggtcagaat ttttcaaaac cttttgaaat ccgattctta gggagggatt caaaacagta
     5641 ttttgcatgg caaacatcct ggggaatttc ttggagagta ataggagctt tgattatgat
     5701 acacggtgac aacaaaggat taattttacc gcctaaaatt gctcctactc aattggtaat
     5761 tgtcccaatt tttaagaaca aagaacgagc tatagttatt gaagaatcaa aaaaattatt
     5821 cgttgaacta aagaatttgg attttagggt tattgttgat gatagagaag aatataccgc
     5881 tggatggaaa tttaacgact gggaagttaa aggtgtacca ctgaggataa acattggaat
     5941 aagagacata aataataaca atttagagat tgtcagaagg gatacaggag aaaaaatctt
     6001 aattaaaaga gtagactgta taaagtcagt acatttctat ctagatcaga ttcaaaaatc
     6061 catgttcgaa aaagctaaga atattcaaga taacaatatt attaaaattt ctaactatga
     6121 ggaaatgaag aaaatattaa atgaaaaggg cggttttatt ttggcagatt ggtgtgaaaa
     6181 agaagaatgt gaaaatagca taaaaaatga gacaggtgca gatattcgac taattccatt
     6241 agaaaaatat tcttcagata acaaatgtat atactgtaat gaaaattcaa aaaagacggt
     6301 aatattcgct aaagcatatt aattatttct gtgcgaaagt tctaatatga ttcctataat
     6361 ttatacttcc acttattgga tatttaatga aattattgac ttaaattaca caaaaaataa
     6421 tcaaatagga gtctcaatct ttggatggca ttaatgatat actcactttt tttgactttg
     6481 gatatgaaga gtatggtaac gtgggaagat attttggaat cttaataatt acgtttttcg
     6541 ctagcataat tgtatttatt cctgttcctt atttccctgt attatttact gcagggatta
     6601 atcagaacct tgatccccat ttagtttcac tttttagtgc agtgggagca gtcatggcaa
     6661 agttaataat attttacgta agctattacg gacgaaatat cttgagcact aaaacgaaaa
     6721 aaaggatgct tcctcttcaa aaacttttgt caaaatatgg ttggccagga gcgtttgctg
     6781 cagctttgac tcctattcca gatgatcttg tttatattcc acttgggtta gccaaatata
     6841 atccagcaaa atttgcgata gctacttttt cgggaaaatt cattctcaat gaatttattg
     6901 tattcggtag tgtcttttta ggcagaccag tggtagaagt attgacttcc gacaatacag
     6961 atccgattta tttgtatata ggggttggcg ttagtattag tatattaata ctgattattt
     7021 tcttatatct tagaattgat tggggaaaga ttattggtaa atggtttcca tggacattaa
     7081 ataacgatga tatggataat agatcaaata ctaaataatt ggagttaccg aataaagaaa
     7141 taatatataa ttaaaacgga agaaaagaat ggtagaacat gaagataatt attatgaatt
     7201 ttcaaaaaga aacagtttaa tcaaaaacga tttaattttt cctttatttg ttgatgaaaa
     7261 taataaattt caagagctaa atgttatgcc aggacttttt agaattccct ataataacat
     7321 tagccaagct ttagaggaaa ttgttgaaaa tggtatacat tctatcattc tttttggtat
     7381 accaagattt cgaaatacta taggtacctc agcaactaaa aaaaatggga tagttcaaaa
     7441 aactctgatg ttgataaaat caaattttgg aaacaaaatc acaactataa gtgatgtatg
     7501 tctctgtcaa tataataaca caggacattg tgggttatta gaaaaggcta aaaaagtagt
     7561 tgataacgac ttaacaaatg aacgattatc tgatattgca ttaagtcatg caatatccgg
     7621 agtagatatt gttgcaccgt catccatgat ggatggacaa gttaaaagaa ttagagaaaa
     7681 attagatgcc aatggttata ataaaataaa aattctatcc ttttctgcaa aacaatcttc
     7741 ctcattgtac aaaccattca gatcagaaac tttttttccc tcatccaatg aaatagataa
     7801 atctacatat caagtaccat attataattc acgggaaata caaagagaaa ttgaaatgga
     7861 tattcatgag ggggcaaata tggttatgat taaaccagca atgtcctctc ttgatctgat
     7921 atatagaata aaaaaaaatt gtacgctccc attagttgtt caagtcgtct cgggggaata
     7981 ttcgataatg aaagcagcgg ctacaattgg tttatttgat gaaacttcgt atgttttaaa
     8041 tttactttct gcgcttaaac gatctggtgc tgataaaatc ataagttatt cttcattaaa
     8101 tatttcgaaa tatcttttat agcctgtgtt atttgggttt attttctgag acacaattat
     8161 atataataaa tatatcaatc gatttatccc gcgatagctc agcctggtag agctttcggc
     8221 tgtagtagtt ggcttattta gctaaccgta ttttcagcct atcaggctta cattagcaga
     8281 aaccgaattg tcgcaggctc aaacaaaata actatttcgt tattatggag caaatcctgc
     8341 tcgcgggatc caaagtttat ggattaaaga cagcccttcc tataattctc cggtttttca
     8401 agtcttccaa agcttgatta gcttcttcta atttatattg tctggtagta acagaactga
     8461 ttttcccttt tttaaacaag gataccaatt ctactaaatc gttaaaccta ccagtgtatg
     8521 cacctgttat agtgaaagcc ctaagtggaa caagaggcaa atttaattct aatgatcccc
     8581 caaagagtcc aaccatgata tatcttcctt tttttcttag gacttcgatt gctgttgaag
     8641 ctgttttact attatttaca aaatctacta atacttcaca tccttttcca ttggtaaaat
     8701 cttttacgct cttgacagga tcagaagaaa gagaatttat tgtcatatct gcacctaatt
     8761 tttttgcttc ttttagtttt tcgtcatcaa catccaatat tataatgttg gaattcgtta
     8821 tcgctttggc aatctgaact gccataagcc ctaatcctcc tgcaccaata atcaaaagat
     8881 tttctccagg catccctaaa gattttttga ttgaagtata tgctgtcaat ccggaacatg
     8941 cgagagcagc agatgagtta aaatctatct cctctaattt aataaggtgt ttataatttg
     9001 gaaccaagat ataatctgaa tacccaccat cttgatatat tccgagggtt ttaggagatt
     9061 cacataagtg ttcgtccccg gctttacagg aatgacaaat ttcatcccca atccaaggat
     9121 ataccaatac attgtctcct gtatgaaagt gaagtacaga ttctcctata tcttcaataa
     9181 cgcctgaaat ctcatggcca ggagttagtg gaaattttac tcccctgtct tctactctca
     9241 taaatttacc tcctgctccc atataacctc cttcccatag atgcaaatca ctatgacaaa
     9301 ttccagcagc ttttaccctt actaaaactt cggaattctt aggctttgga ataggaactt
     9361 tttcaataat aagaggttca ttcgttttta caattctagc agatatcatc aattatctaa
     9421 aatctaagca taataaaaat ggaaagacgt caaaaaattt tttataaaat aggaagataa
     9481 ttacataatg tatttattaa gatttagtaa agtaaagata actatacaaa caccgatgaa
     9541 gtataggaag agccgtctga tacgttgtat atgaagaaga ttatgagtta ctgaggtgtt
     9601 agtaattgta aaaaattaaa attgttataa attttcaaac aaattgtctt cattaattgt
     9661 caaatgtttt tttattatag aaaatttact gttgtaaagc atatagaaat ggaggaaagg
     9721 gcaaataaat gaaaaatcgg gcagatattg aagtaatcgc gtcaattctt cgaagtgcaa
     9781 aagataaatg ggaatatcaa actactataa tgaataaagc atcagtatct cactctcaag
     9841 ttattcgata tatatccgtg gcgatgaata cacatctgat cgaatattca aaaataactg
     9901 gattatataa aaccactaaa atggggttag attttttaga aaaacatggt aaattaataa
     9961 tcctatttcc tgagatcgca gaaccttccc aatgttctta attagaagaa ttggaccttg
    10021 tattttattt agtattttaa taaatactag taattgtttt taatttttgt atttgagagt
    10081 agcatgttta ttttctggag gaaaggatag tacttattct ttatatttgg ctaaacaatg
    10141 gggactatca gttgattgtc tcttaacttt aatacctcat tccgttgaaa gttatttatt
    10201 tcattctcca aatatctgga cgacacagtt acagtctcaa gcattacaaa ttccaattat
    10261 tatgaaacaa atcaattcta tatcgataga tgacgaatgc gacaaattag atgaattaat
    10321 caatcaagca aacaaggatt ataaaattga tggaattgtt catggtggaa taagttcgaa
    10381 tttccaaaaa aataaattta acaagatatg tgaaagatac aacttaaaac tcatttctcc
    10441 attatggcaa attgatcagt atgattacat gaataaactg ctgaaaaata atttcgtaat
    10501 tcaaattata agtgtatctt caatgggtct agatgttaat tctcttggga ttatactaaa
    10561 tcattcaacc ttgcaggact tatttaagtt gagtagaaaa tatgggttca acatctcttt
    10621 tgaaggaggc gaggcagaaa caatagtttt agattgccct attttcagaa aaagattaga
    10681 aattttaacc tcaaaaatac aatgggatgg acaaagagga atagtagaaa tatcggatat
    10741 cgttttatta gataagtaaa tgttagataa tctgaggaca gggttaagaa gtgctttaaa
    10801 gaaaatagtt ggggcatctg atattaacga agaacttata gattcgctgt ctaaggatat
    10861 tcaaagatcc ttattacaat ctgatgttaa tgtaagacaa gttctttcta taacaaaaaa
    10921 tctcaaggaa cgagcgttaa aagaacagcc accaaaagga ttaacaaaaa aagatcatat
    10981 aatcacaatt ttatacaatg aaatttcttc tctgttggga tataaaggca cccagattct
    11041 ccatatagac aaagctcaat caatcccaga agattctttt aattttcaac caggtaaaaa
    11101 aaatataata ttgatgttcg gaattcaggg aagtggcaaa acaacatgta ccgtaaaaat
    11161 tgctagatgg ctttcacgac atggctataa ggtagcaatc ataggggctg atacttggag
    11221 accaggcgct cttacacagc ttaaaatgaa tagtataaag attaaagttg aagtatatgg
    11281 cgaagaagaa aataaagatc ctatttcaat agtaaagaat gggttagaat attataaatc
    11341 tcaagattat gacgtgattg ttatcgacac cgcaggaaga cataaggaag aatctggatt
    11401 attggaagag atgaatgaaa tacataaggt tacaaatcct gatctagttc ttttagtcat
    11461 agatggaaca attggccagc aagcttatag tcaagcaaaa gcatttcatg atgccgcccc
    11521 aattgggggg atagtaataa caaaactaga tggaactgca aagggtggtg gtgctctagc
    11581 cgcatccgca gttacaggag cacaaatctc ttttataggt acaggcgaac gtatagacga
    11641 tctggaatta ttctcaccaa ctaggtttgt tggacgattg cttggtatgg gtgacattaa
    11701 agctctcctc gatatggcta agagccttga gattgaagca gatgaaactc aatctaagag
    11761 gcttttagga ggcaaaatga caatagagga tttctattct caaatggaaa atgttaataa
    11821 gatgggattt agaaacgtta tagaaaattt acctgggtta tctggaatgg taaaagatga
    11881 acagttggat gtattacaag gaaaaattga aaaatggaga tttataatac agtcgatgac
    11941 caaagttgag aaaaaagatc ctaatctcat taatgatgcg agaagaaaga gaatttctcg
    12001 tggttctgga ataacagaac atgaggtgaa agatatgata aaacaatata acaattcaaa
    12061 aaccatgatg aaacaagcaa aaggaagaca gatgcatggc atgttgagac gatttggttt
    12121 tggctaaatt ttgactagat caaaatattt gaattattca aacacattca gttctgaata
    12181 tacactatgt aatatttgtc agaaacggaa tttccctact ttaaaacaat ataacaaaat
    12241 aagtaacata aattgcttta tctgcaaagg tatttttagc aatattaatg aaattgcata
    12301 cacactattt gatatgatta aatttcaaga aaactatagc tatcgctcat ttacaattgg
    12361 aagctcatta ccttatttta tgtttgataa agaagattat attaggtcac gttataaatt
    12421 aaaagataga gaagatataa aatcttcttt taattttgaa attagaaaca aatttaaaac
    12481 tctatcaaag aaaaaattaa atctaatcaa tcctgacatt aaaattaata tatttttttc
    12541 tggtaataat gaatattcta ttactatttt atccaggtct ttatatttgc ttggtagata
    12601 taacaagaaa aagtttatgg ctcaaaggga aaaaaatagt ttaaagaatg atcctaatgg
    12661 taatcatgat gagattattt caaaaaaaca aacaattgaa acaataataa aaaagacttt
    12721 atcaaattgt attagatcag ataggatggt tttcacgtgg tatggaagtg aagatgataa
    12781 tagccaagta ttaggtaaag gtcgcttatt tttgattcag cttatcaatc cacgtgaaag
    12841 gacaataaag attaataaag tttttactga tgacggaatg tcattcaaaa taattgaaaa
    12901 aagggacttc ttcggaaata ttaaaaggaa ttttagaatt aaaaataaaa tatttataaa
    12961 agcatctaat cctatagata aaattcatct tatcaaatta atgaatttaa ataattctat
    13021 cataagttat cgttacaagt caaaaacaat acataaaaaa atctattata ttaaattcaa
    13081 aaaacttgac cctctccatt tgataattaa tatagaatgt gatagcggtc ttttcttaag
    13141 acagtttgtc gaaggaagaa atctcattga gccaaatatt tcattagtac ttcatactca
    13201 atgtgaatgt ttaaaattcg atattatgga catctcagtt tcttaatttt ttatacaaaa
    13261 caatgtagtc acctttgtta acgaatttga tcaactggcg aaagtcaaat ctgcgaattc
    13321 ttacaataaa atttctaagt tgtttataat tttatattga aaaaatttac aattgtgaaa
    13381 gaaattctaa aactcatttg aaaacattaa tacgatacaa ttcctttatt tttcaataga
    13441 atctaattag ttatttcata ctctcaaatt gaatttataa aatacctata ctgtacgcga
    13501 ggcctcttcc gacttgagat attgaattaa aattggatac tctaattcct attactgttc
    13561 atttacttat gtaggaaaaa agtctatcat cccgatagga ggtcagggat ttacatatct
    13621 tcatttttta tcgtaacatg gttgatggtt ttcatcaagg aatttatgaa ggcatgaagc
    13681 atctattcta tgcaaatata gaatctcgga atgaaaaaac atttttgaga atatgctgga
    13741 taattttgaa tacggattta cagatgctac tttatacact aaaataatac aaaaatggat
    13801 taataaaggc tacttattac aaaattttat gaagaagata ataatttgaa gaacccacta
    13861 tatgatgact ttttcattaa ttttgttttg aaaaaaaatt agtaatttta gaaaccctaa
    13921 ttataaatga taaaataaaa tttctctaga ctatagtttt ttcttgataa attcagtaaa
    13981 gccacattta ccacatgtta gtctatcttt atgggcagcc atgaatacac cctttccaca
    14041 tctaggacaa tccctcttga tacgttccac agaattacca tctaatttat agaacttgta
    14101 aacttgagag tctttctcgt tttttttatc tgtcattaat ttcaccaata ttatttagta
    14161 tctgccttag cagcttgttt ggctttcaat tttacagcct tttcttcatc aattattttc
    14221 tttctatcct cttttgataa ttttctaagt attctatact ttggaagatg tttttttgct
    14281 atttcaccat tttcgtagat aaaaagagta gctgtaagat cagtttttcc tttttcacca
    14341 gctaatgaaa tcggaaaaat ttttttttca tcctcattaa aattttttga tatcatcttg
    14401 actaactcga ttttactaat tttacctgct gcactcttca agaaaatttt tagttcccgc
    14461 ctatttaaca tgaagtttaa tttatcttct aatatagtga attggaagct caatgtcgta
    14521 tcatattttt acaaactcat ataagcattt gcattatagc agaatttact ttagataatg
    14581 actatttaac tagtaatgat ttagattttt atatttagtt tttttataat ggtatatttc
    14641 caagccaaaa tgtttatctt gttatagtaa ggaggaaagt aatgatagta gtttatttta
    14701 tgactgtaat ctcttcaatg ttacgagacg tatggtaatc gtaatgagat tagctgaaaa
    14761 aatttacttt aattttgtat tgggttttgc aatcaataaa taaacaaaca tgtctgaata
    14821 gattcaaaag tgatattgta tatgtatttc taatacctat ttttaatttt taacttagat
    14881 ccaaatcctt ttcaatttcc aataatctat ctttattccc acaaaatgta attaaatctt
    14941 ttaatttttt tctggctttt ccttctctaa ttacagatct ggaaatatca attccttctt
    15001 taatgtcttt agcgatctca gatagaacca atgcaagtgc ggaattcatg gctaaaatat
    15061 cttcgatttc cttgcgagct aacccataaa tagattttag agtcaggtct attgaatcct
    15121 tcttagtttt aatcgtaata ttatttaatg aggtaagggg tattccaacc tcttgtgggt
    15181 tgaggttaaa tctgtggatt tctcctttgt taacatagat tatcttgctt aggcaagtat
    15241 tagatatttc atcaatgcca tcaatcgaat tcataagtat gtatttttta tttagttttt
    15301 ttgtaacttt ggaaaattta tctaataatg ccgcatcata cacacctatg atttggccac
    15361 ttaagttatc acatggatta cataaggggg ctgcaatatt aaaaattgtt ttcatgccta
    15421 tttccttcct taattttcca acatttttta atacagggtg aaataatgga gcaaacaaaa
    15481 aacctatacc taattcttca atactacgtg atacaatatg aggagaattg cctaagttta
    15541 gtccaatata ttccaagaaa tcagcacttc cacaaaaacc agaagctgac cgatttccat
    15601 gttttgctat attacatccg gcagaggcag caacaattgc agatgcagta ctaatgttaa
    15661 atgttttgat tttagatcct ccagtaccgc aaatatcaaa taaattattt ttgaactttg
    15721 gatggattgt tagagaagac tccttaataa tttgaaccat tgcaagaatt tcatctactg
    15781 tctctccttt caatgaaatc tccttgagaa tagaactagc atacgaatct tgcattctcc
    15841 cttcaactat atttttgaat atcactttag cttccttaaa tgataaattt tcttctgaag
    15901 aaaatttatt taaaattttc tgaatattaa gttctatatt ggtcattttt ttataaataa
    15961 aatgaaatta tttaatatct ttaatccctc attagttaat attgattctg gatgaaattg
    16021 aacccctttt atagggtatt ttttatgagt tattgccata atctcattat catccaatga
    16081 attcgctgtt atatctaaac aattaggaaa tgtttcctta tcggctatga gagaatgata
    16141 acgagtcgcg acaaaaggat tcttgacatc ttcaaataag ggatcatctt tataatatat
    16201 ttcacttgtt ttgccgtgcc gggtggtaac tgcgttatta atttttccac cgaaagctga
    16261 tactatccct tgatgtccca aacatatacc taaaagtgga atttcttttc ctaaaatttt
    16321 tataatatta ttgcaaattc caaagtattt cttatcttca ggatttcctg gtccaggtga
    16381 tatgattatt gcatctatat tcatcttttt tattttatta agtgtgatag tgtcatttct
    16441 atggacgtaa gttgtaatat tggatcgtcc aagtgattgt gcaatattat aaacaaaaga
    16501 atcataatta tcaataatca aaatattcat ttagattagc gataattaat aagatgagtc
    16561 aatttaaaca tttcaaaata ttataaagtc aaaataaaaa tatattatac gtttattgga
    16621 ataacttaag aagattttaa tggttgaaat ggttaaatta tttattaatg gttatggtaa
    16681 tatcggacga agattagcta cagcatttaa cttagatgaa gaagtagatc tggttggtat
    16741 agcgaaatat aaggccgacg aaagagcaaa agaagcaata tccaaaggat ataaagtttt
    16801 tatccccccc tctctagaac aagaattcaa gaataataaa ttggagttta ccggttttat
    16861 cgaggaggct attcaaaaaa gtgatattgt aatagatgca tcgaaagaag ggctgggatt
    16921 tgataataaa caaaattttt atcttccgat gaaaaaaatg gctatatttc aaggtggaga
    16981 agatagagaa ggcaaacatt ctgttgctga cataattcac aattcaagag ttaattatga
    17041 gaaaatggtt ggtaagtcgt atgttattca gggtagttgt aatgtctcag gaatggggag
    17101 agttattcaa ccgttaattg atcagtttgg tgacagaata agtagatacg atgtaacatt
    17161 gattagacga tgggcagatt tggaggatct aagtgaggtg aaggattcta tacaatggga
    17221 taagaatcct catcatcaag atgatgtgaa aaattttata cctaacatcg aactatttgt
    17281 agatgcctat aaggtacctt ctagaatgat gcacttacat caaatgtata taagatttaa
    17341 agataaagca cccacaaaaa atgaagtact agaaattttc tcaaatgagt ttggggtagc
    17401 aattttaaat tctgcgaagg gaactgctga tataagaaaa aaggcgcttg agatgggatt
    17461 tatacatggt gataccaata tgattcatat tcatcaagaa ataatgagaa gccaaggtga
    17521 tgtacttaaa atctcctatt ctgatgacca aacaggaatg gtgattcctg aaaaccatct
    17581 tcttgttcag tctctctttt ttaagagatc tagaaaaaaa gccctttctc atactgataa
    17641 aatttttgag ctcagtaaaa agaagaaagt attgcaagaa gaatttaaat aatttttaga
    17701 gaatcgtcat tgcttataaa atttaatttt atttgatcta tagatttgtt ccaactaatt
    17761 gtttatacag gagtaatttt tattattgta cttttgaact atctcaggaa atattcttaa
    17821 aatttttgaa agataaatac taagtaaaag aacagattac ctctatgaga aaaaaattaa
    17881 gaaaggaatt attattttcg taacacatcg acttggccac tatgaagcca atcttttagt
    17941 tcatcgtgag aaggtttcgc atcatgtttt cctgctaaat gaagatgtaa aagaacatag
    18001 tttacctggt tcaataaggg tttattattt tcatctcctt ttctagcttt ttgctttaaa
    18061 tctgcaggta cagaattcca ccagtcatta aaatttcttt ttccattttg acttacattt
    18121 gatccaaaac cttttggcat tgtcataata ttttatcaag atatcatcta tattttaaac
    18181 ttattgattg taataaactg aaattttaaa gagtaaacat gttccgaatt tataagatgt
    18241 taggttccaa atatcttttc ctatgcagag attaatatta taaatcattc agaaccttta
    18301 attaaaggaa accatgacgt ttaaaagcat taccttggga gaaatgagaa aggactatgt
    18361 aatggataaa ttcgtaatct atccaaataa tttagaggaa ataaaaaatg ataattcttc
    18421 actcacaatt tgtccatatt gtcccggcaa tgaaaataac acaccctctg caatagtctc
    18481 acttgtaatt aaaagtggaa ttctccagcg attatccgat tctgaaggaa acatagttaa
    18541 agattggagt gtaagggtat tcaatagtaa tacccctata atagttcaaa ataactctac
    18601 tctgtatact gataaaccgc tatatagcga accagcttat ggctatcatc aagttttagt
    18661 agcaactcct gaccacagtg atagcttctc caaaatttca gtggagcaat ggtgtaatgt
    18721 gcttttagtt ttacaggata gaataagatg gctctattct caaaagaaag tcacgtatgt
    18781 ttctgtttat ataaataacg gaaataggtg tggatcaaaa attgatcatt ctcatattga
    18841 cattgttagt ttttcggagg ttcctccatt tgtagaatct aaggcattat catcacatcg
    18901 atatgttaat gaaaatggaa attgtccttc atgtattgcg atttctactg aactagatgg
    18961 acctagacaa atacttgcca cagattattt tattgctttt tgtccctggg cttcgattta
    19021 tccttatgaa tttgcaattt atccaaagaa acattctaca accgtgacaa aaatatctca
    19081 gaaagaaata tttgacctcg ctatgattct aagatccact ttaggaggaa tggctcaagt
    19141 attaaacaat ccatctttta atcttatatt tcaactatca ccagaaaaga agaatagcag
    19201 acaatttcat tggcatatcg aaatctttcc acatatctct gaacttactg gattagaaaa
    19261 gggatttggt gttttcgtga ataacgtttt accagaaacg gccgcctcaa aattgggtgc
    19321 ggctgcaaga aaagaaattg caaagatagt aggagttgaa taagtgatct taaaaaatag
    19381 cctcgattta gagagttggt tgtccatagc ttctctgggg ctagcaataa tgtttgtttt
    19441 attaattgtt tctttttata atgttttaat cagtaatgaa ggacaaggac cggagagatt
    19501 tgttgacatt cgtggagtgg ttgtcaagac gctatctatt tctggtgcac cttccattat
    19561 cttagcagcg atatcatttg gtttatcaaa aaactatggt aatagattga ttggaatgct
    19621 attagctgcc tctggaatag ttttaatcat aggtatggta atctctcttt ctattagctc
    19681 taatatcaat tctgaatttt ttcatcctat tcttgtttta gttccttata cttttatagt
    19741 cggaggaata gcaatattta ttattggagt tattgtttat ttaaaaacta aaaaaggaat
    19801 taaaaaaaca gggagattta attgaagact acaaatattg agaagattga gatatcgaga
    19861 caaaatttac ctattaacgt aaatagtacg ttactaagag atatttttaa tttagctaaa
    19921 ttagaaaata gtccaatact ttccgtggtt atcggaacaa aaccagattt ctataaacaa
    19981 gcgccattag ttgtcgaggc caaaagagaa aaactccccg tatttgttat agatacaggc
    20041 cagcattttg acgagatttt aggttttgga ataaaggaat ttaaattaga agaatcagtt
    20101 ggctgtgact tgcagataag aggagatctt atggaaaaag ccagtgaatt gatacttaaa
    20161 tttggatctt ttggtagatt ttgtaaagaa gaatttggtt ctacctcaaa actactccct
    20221 gttgttcacg gagatacttt agttgcagga atagctccat tggcttgggt ttttggaatg
    20281 ggtcaaaagg tcgctcaaaa tgaggcagga ttaaggtcta tgagtccaga aataatgaaa
    20341 aaattaaaaa ttacacaaga accgacatta gatctcatag aaaaatatat tcaagaacaa
    20401 tttgatggaa aatggtttgt cgcaagagaa gaaccatttc ccgaacaaat cgatacttgg
    20461 atatgttctg caggtacaca gtattttttt gctcctaccc aattgaataa ggataatctt
    20521 gtcaaagaag gataccctga tgaatttgtc catgttgttg gtaattcagt ggtagatgca
    20581 atctatctta aaaggaaaca aaaagtgtct caaagtattt ttgatttata tccagaatta
    20641 gaaaaaggaa aatggataag aatggatatt catagaaggg aaaatctcac tgaacatcga
    20701 tttaagtcaa taatccatgg attagtaaat atcgttaagt attcagatca caaagttgta
    20761 ttagttatgc tcaatgctac taagtctgca ttgaaatttt atggtttaga agaaaaagtt
    20821 agattactag cagatgaatt tcctcacaaa ttcttaatca ctcctttatg gaaagaatat
    20881 ggtcaagtaa tagagttctt ggatagcgga cgatgttggg cagagatgac agattcaggt
    20941 tccatgcaag aggaattatt atattttaaa aacgtattat ctcttactat aaggttaaac
    21001 acagacaggc ctgaaacaat ttttgatgct aaaagcaatt tgctgattcc cccaataaat
    21061 tctgaatgga ttacctcaat ggtgaatcat ttgtatgata aatttgaaga ttttggagta
    21121 aagtcaaaaa ataagaagga gatttatggt gaacctggtc aagtctctaa aacaattgcc
    21181 aaaataatta agaaacaatt tgaagataag aatacaagtt tttatccttg gtttcatcaa
    21241 aggataaatt tatggaaaga agataatgat ctacaatata tgtagtgtga acgttataat
    21301 ccattaaata aagcattgta gattgttgtt gatatctttt atgcttaaat ataatacgct
    21361 aataaatttt attgattagc taaaaatcat attgttatat cgatacctgt ttaaattgta
    21421 actaacgttt aatactccaa attcaaaatt atagttgtaa tctatcttaa ctattcttag
    21481 ataaattaat taatgtgaaa atgataaaat aaagaggccc tcttttgtca aatggtaata
    21541 atggagaaaa tctattagga agagatttaa tatcaagaca aaatagtttt aactgtataa
    21601 ttgtactgtg tatcgatcga gatgatgatc taggatcaaa aggtggtatt gatacgcctg
    21661 ttattgggag ggattcatgc atcaacgcgg gaattcgttt agcaattgaa gatcctgaag
    21721 attccgatgc gaatgctata tttgcagctg taaaaactta cgaagatcta ttgaagagag
    21781 gatataatgt ggaggttgcc cttgtggcag gaagatataa tagggggatt gaaggcgatg
    21841 aaaaaatcgg ctctgaaata aaatatatcg ttgataaatt caaagctgac gcttctgtaa
    21901 ttgtttcgga cggtgaagat gatgagactg tcgtaccggt aatccaatct atggtgccaa
    21961 taatatctgt acaaagggtg gtaattaaac atagtagaag tgtagagtac tcttatgcaa
    22021 tttttgcaaa atatatcaaa atgttggttt atgaccctag gtattccaaa ttctttctgg
    22081 gtgttccagg tacattgtta gtagcgagtg gaatagctac tatttttggt ttgacaaggg
    22141 aagctattgc attagttcta agcattttag gttccgcgtt tatcatacgt gcatttgaca
    22201 tcgataaatc aataagtgca ttaggtaagc caactcctac tagttttatc aggatttttt
    22261 ctttggtttc tggattatta attatagtag tttctatagt gaatggtctt tcacatatcc
    22321 cagagggaat tgttagggag gatatgacaa attttgaaat tgttaccaac aaagaaatag
    22381 ttggaagttt cctaagaggg actattttgc tattgtggat tggaatcggc actatattat
    22441 ctggtactct attaagtaat tggtttaggg gaagtatcaa aaccatgtca gatattttaa
    22501 gattggtgat attgatttta ttatactacc caatcttaca atttacgtca gtactaactg
    22561 aaggaactag tccttttaat cttatttctt cattactaat tggattagct ataacattgg
    22621 ttgcagccac atttctatat caatattata gaaatagaaa aggtggtgaa tcgcttaaga
    22681 attagtattt aataaaataa aataaataag tattaaacac tttataattc aaagaaaaat
    22741 attaaatgaa aatggtaaat ttttctagtc aaattttgtt tgaattacta tttaagttat
    22801 caggtttata taaaatatat gaaagttggt tggagtctca gatatcaaga gatcctctac
    22861 caaagcatgt tgctattatt ttagacggaa accgaagatg ggctaaatat catttttttg
    22921 gaattgacaa agctcacttt attggtgcgg aaaaagctga agaattgtta aactggatat
    22981 atgatttaga aataaaaatt accactgtct atgttctttc ggacgagaat cttgaacggg
    23041 aaaatgagga attaactaat atctataact tattgagtaa aaaattaaca aaacttaaag
    23101 atgataaccg aattcataaa cgaaaaatga aaattaaagc tataggtaat atagaattat
    23161 tgcctaaaac cttgcaacaa atactaaaaa ttttagaaga agaaacttct tcgtacaatt
    23221 caatgttttt aaatatcgct gtggcatatg gtggacaaca agaattagta gatgctatta
    23281 aaaaaatagc ttcgatggtt acttctggaa aattaaaagt taatgacatt aataaaaaaa
    23341 tcattgaatc atgtctctat acttctcatc ttccacaatc ggacccagac ctaatacttc
    23401 gaacttcagg agaaaaaaga ttaagtggtt ttttactttg gcaaagtgca tatagtgaat
    23461 tggtatttat ggatgttttt tggccagagt tccgaaaaat cgattttatg cgggctataa
    23521 ggacttatca aaatcgtata aggagatttg gaaaataaag taacaactaa aataatgtac
    23581 agtttctcta tttatcaaat gtatgaacat ggttattatg attctcattt aaaaaaatgg
    23641 cattgtggtt attttatgga cgaagaagaa tggcttgata tacatgatta cttaccgaac
    23701 caatttaaag aatatttttc caatagtgaa tcttcagaag aagaaaatga taaaatagca
    23761 tgactttgct atggtaatta tttgacaagt gaaatttctt gtggagcatt agtctattgt
    23821 attgaaaatc acaaaataat attccttcta ttacactatt tccatggtca ttgggatttt
    23881 cctaaaggaa ataaggaaag aggagaaaat catatggaga ctaccagaag agaaattaga
    23941 gaagagacag gtattggcga tattactttt attgacggat ttgagaaaca gatatcttat
    24001 aaatataaac gtcagagtga gttgatctct aaaaaagtaa tttatctttt agctagaacc
    24061 atttctaaag acgttgtatt gtcatcagag catacggctt tcgtatggga accctatgaa
    24121 aatgcgttaa aaaagcttac ttataaacac tctaaggaaa ttctcaccga aggttattgt
    24181 tttataaaaa attttatcaa ttctgctact taaataaaat cgatcatata attgaacaga
    24241 aaaattattt tatttgaggt tagattatat ttataatgtt tttaaatgat aaacactacc
    24301 atatattcaa aaacgaactt cacctttctg agttggaaat aaagatattc ttgctaataa
    24361 taagccaagg taggttgaaa caacaacaga tttctttaga gttgaatttg gataagtcaa
    24421 actgtagaga aattatagaa tcactcatta ataaaaatat gataattgaa tattcaaata
    24481 atttatttga atgctttcat cctagatttg ctataataaa tagatataag agattgtgtg
    24541 ttgaaaagga tattccctgg aaaaaaaatt cagtaattga taatcttgca atcatgctag
    24601 aacaatcatt tgaggctgca agaactaaat aaggagaaat aaaaatatta tgttctatga
    24661 gtgaaaatga ttttaaaaca aatgacaaga tcgtttattt tggtgtcatt aatataaatg
    24721 aggaaggtaa aaccataggc gcagtagata tttggagaag tgttatcact aaagagattt
    24781 tctgcgaaga aaaaagatta ggaattttag atatagcaga ttatattgga atgcctagaa
    24841 tagaaaaagg gaacaaatgg gcagttgctg ttaatagaaa tcgttttggc aaagatagat
    24901 ggaaattaat aaaaattttg gaggaaggaa catttaaatt ctatgatact gatgatgaga
    24961 caattataaa tacaaatgta caaaatttcc atataattga taatgattgg tggaatttcc
    25021 ttgttgaaag caatataaat cggagcgtcg aaattacaaa agagttatag acaatgtatg
    25081 gatttggaga taatgaggat cttaaaatag atgtttttat aaataattcc tcaggaggag
    25141 aagttgcaag taaattgacg ggttattttg ttgttaaaaa caaaactttc agattttctg
    25201 ctattgcatt tgggagaatt ggaggtcata atatcagctt aaaattatcg aatgcgtcta
    25261 ttaacaaatt aaaaaaaatg gaaattgatc cagaaaaatt acaacttact atacagagaa
    25321 agttaattga aggagatgtt aatcttcctg caaatatgaa attaccatga taaataggaa
    25381 tacctattct taaggaagat tttaaggtta atgagaaaca tttctatact taacaattgg
    25441 tgttagtaaa aaattataat accgcagcga ttcattaaat taaagcgttt tctaatgagg
    25501 ttgaacaatt acataaattc ctatcctctt ttattttggg aataattgtt agcagcattt
    25561 tttttgtttt atcaacgttt ttacttaatg tttcaattat ttcctgtgaa tttacagaaa
    25621 cttcagccca aacatcataa tcagttactg ttgagatgga cacataacac atctccattt
    25681 ctcttgctaa aatacattct ggaattaagg tcattcctat tatgtcggct ttaagtactg
    25741 tcctatagaa attagattca gctttagtag aaaatcttgg gccttctata caaatatacg
    25801 ttcctactgg atgaaactta aaccctaatt gttttaaatc tcttacaatg ttttctctca
    25861 aatcaggaca aaatggatca gattgagaaa tatggcaaac taccggacca tcgtaataag
    25921 tatatctcct tttttttgta aaatcaatat attgatcagg caatattata tcccctggcc
    25981 tatattgtga tttgagactt cctactgctg atggtgctag aattacttta acaccaagtt
    26041 cattcatagc ccaaatgttg gcttgataat taactttgtg cggaggaatt ctatgatttt
    26101 ttccatggcg aggaataaat gctatacttt tttcattata tgtacaaatt gttattctat
    26161 ctgatgtttc tccataaggt gtattaattt tgatttcatc aattatatta aaaatattag
    26221 agtcatagat acctgttcca ccaataactc ctattgttga tctgtatttt aaatttttaa
    26281 tcataatatt tcaccaatga acgaacatcg tacccttttt ttcttaacat ttctgctcct
    26341 tttaggtata ctaattctat aataactgca aaaccaaaaa ctctccctcc catttcctcg
    26401 attaattcag cagttgcact ggcagttcca cctgaagcaa ttaagtcatc agcaattaga
    26461 atattttgat ctttttgtat tgcatcttcc tgtatttcaa tcactgaact accgtattca
    26521 atatcatact ttttttttat tgttttccca ggaagcttgc cgactttcct cacgagtatc
    26581 attcctttat taaaattttt agctaataaa gatgcaacag taaatcctct agactcaatt
    26641 cctgctatca aatcaatttt tgacaactct tcttttttaa taaattcttt agcgatcgct
    26701 tgtattgcaa ttgaattcct gaacaatgga tttatatctc taaaaatcac cccttctttg
    26761 ggaaaatcat taaagttttt gattaaagat ttgatgttta tcttatgatt gtacaacatc
    26821 acaaaaatta tcttagcata taaaaaacct tcctctaatt ataaaaaatt ttttatagta
    26881 ttaatatacc tacgttccct caaaattata atattatcgt tttaacagtt attaatagta
    26941 gggcctccag tgcaagtttt tcatgatatt aattagattc aatttggaat gctgaatttt
    27001 ttcccctgga tattttcaaa tgaagacatt acaggattat agtatcacct atgaaaagag
    27061 tgctatatct ttgacgtaac taggtattat taacaataac ctaaagatac tagatggata
    27121 caatcctcat ttccattccg taaattcaag ttttgctaag ttaatataag catttttttt
    27181 atttaaatta tgtaaaatag aatggcgtaa gttcgcttct atgaaaattg tatttgcaag
    27241 attgctatta ctcaagtctg aattttgtaa atttgcccac ataaaattag atctcttcat
    27301 attacagttt gttaataaac attcgttcat ttctgcatac atgaataatg catctgtgag
    27361 atttgagttt tgaaaattcg aattattgag ttcggcttgc actaaattgg tttttttcaa
    27421 tctgcaatta gaaaaattag ttttattaca aagtgctcca ttcagaaaag ctaaggaaag
    27481 attttttccg gaaaaatctt gcttatctaa attgggttta aaggtaagat tatgaattcg
    27541 caatgtatta aattcattaa ttttttcagc atcaagaagt tcgatccctt catcgttcaa
    27601 atttttcatt ggataaaaaa caaataactg ctatatcaat tttacaaata tcaaatcatt
    27661 ttaatttttt ctagtttatt aaatcgaacg gaatgactca attatagaat agataacatt
    27721 tttagtagga gcttataaat aaaataggcg tgaacttctc taaaactact catataccgc
    27781 ctaggataat ggttgttgat gatgaaagag atattttatc tattataaaa agaggacttg
    27841 aatcaaagaa cagattccaa gtagaaacat tcattgatgc agaatcagct ttggaatctc
    27901 ttaaggaaaa tcctgatgat tattatgatt tggttcttac agatatccga atgcctaaaa
    27961 ttaatggatt tgaattatat cgacggatta aggaaagtaa tcctcatatg aaaatagtat
    28021 ttataactgc ttttgaaata aataaagaag aattcagtaa agttattcct tcattagatg
    28081 ttatagactt tataagtaag cctgttagta tgtctacttt aatgactaaa ttaaattcta
    28141 ttttgaatca aattaactga ttttttaata gttagtcatg atttgttatt attaatctgt
    28201 tttcaatcaa ttccaactat tggtaaggcc tttttcgaag gaaaattaaa actgttagtg
    28261 tcaacctctt cttttgtaac tttttcaaat tctaacgtta tttcgacagg aattgaaaac
    28321 tttaaactaa tctcattggc taattgacct atattataag ggtcatgaat ttctttagat
    28381 ccatcaagaa atactcgtga ttgccttctt aaaattttgc caccaaatgt tgatactaaa
    28441 taatctgtta actctctaac tattttttct gggatctcat tatagtaaaa acaaatacca
    28501 tgaacaggct ctttatcgaa aggggttcca tatttatacc aaaatattcc aaaatgttca
    28561 tgttctccat ataagcactt tatctcccaa tggttgactt tatcagtggg gtatttcgca
    28621 tttaaaattt cagatatagt aagatgccaa tatcttagac gcataagaat atctaatgtt
    28681 acaaatataa taacttatga aagattaata agaagattta gtttaaacca agaacaaatt
    28741 taacttgaag attaattata atgaaaatta cgataggtta aaattaatag gagaaatgga
    28801 aaaggaattt aacaataaaa acattcaatt tttcagtaaa ttagcattcc atcacgatcc
    28861 tgtcattagg actagatcta tttgtattat tgctgatatt ggcgaggaga aaggggtaga
    28921 caccataagt aaagcattaa aggaagatgt tgatgactta gtaagacatg aggctgcttt
    28981 ttctcttgga cagttaggat ttaagagagg gataaagcca ttatcttatg cagtagaaaa
    29041 tgatccgagt atttttgttc gacatgaagc tgctgttgct ttgggagtga taggttctga
    29101 ggctgccaga tctgtattgg tgaccgcatt aagtgaccca agtgaagaag taaaaaagtc
    29161 agctattgtt gcattggcaa acctagatta tatacttaac tctaagagaa taaacaaatt
    29221 tactcgaatg actggaggat agtgaataag ggaaataaga atcaggttat tatttcgtgt
    29281 actttatttg atttaattgc ttctttaatt tctctgctta ttcgtcttcc catactcata
    29341 ggttcattat ataaaagatc tgaataagga gatccattaa taaagagatt tgtaccagct
    29401 acgattctgg cagaaaattc aaatataaca aatttgccat ttttgtcata aactccttct
    29461 aagcagaaag gaccagggat gccaggggga attaattctt ttgatttttt agcaaaattt
    29521 tcagccattg taaagacttc ttgtaataac gactctctaa gtaccaaggg gaaattacca
    29581 ataacgttat aggaaggatc aagatcaagt tgaatttgaa tatgggctgg tattctcccg
    29641 atgccatcaa tgtcactttc atatcttcga tctataccta atagttcgac ttcgtctgtt
    29701 aaaggagaat aaaaaaactg taaaaatata ggtacaccta atacatattc ttgaatatat
    29761 aactcctttt cagactttat tactttttga ttaattaact ttttacaacc gttatcaaaa
    29821 ctgtgttcgt tccaggcaag aaaatagcct ttcccacctg cagccccatg taatttaact
    29881 atacataaat ttttaatttc attttttcta ttaatttcgt tgggtgtatt aaaatttgat
    29941 ttattcatca atttgtgttt taatactcta tctgcttccc atcttaatat ccatttgtta
    30001 ccaaatactg gtatattaat tttttctaac tcttcaaatt tgacattgga tattaaagtt
    30061 ccatgcggaa ttagaattat ttttttatct attaattcat cttgaaccgt tttgcttaat
    30121 aaatctccaa aattatcaag aaaaaatata tcatcaataa atttatacct tttatataga
    30181 cttgcccgtt ttttttcaca tattaacaat gttttgaagc cttcatcttt tgcacctttt
    30241 aaaacttgta aagaacaatg agaaccaagt gtgctaataa taggatctct tgtatctatt
    30301 gcattcgaca acattttcaa atgttattta gggtgataaa ttatttatga tttacaaagt
    30361 ccctcaattc ccaatctcta atataattta aaaaaaaaca aatattgcct tatgttttag
    30421 aaatttatga ggactaaaaa aaacgttaaa aaaattaata tgaatgaaga tatattattt
    30481 gataataata tttcacaatt actattaagc ttagatgata aaatacgttc tggtgaatat
    30541 aaaattgcaa tatatggtct gggtcatgtt ggttctccga ttgctgcggc atggttacgt
    30601 tcaggagcgc atgtaatagg cgttgataaa tctaaatcta ttttacaata tgcaagagaa
    30661 ggaaaaacgc atattcctga acctggagtt aacgaagcat ttgaaaaggg gattaacaat
    30721 cagaaattat ctatatatga tgatctagtt aaagcgtcta aagattcttt tctcaagatg
    30781 atatgtgttc ctgttcttgc tgctgatgga acagctgatt tatctgcggt taagaacgta
    30841 gcagtagcaa taggaagggg tttaaagaaa aatgatgttg tagctattaa tccaagtgtt
    30901 cctcctggta ccactgagga agtagtgatt cctatattac aaaaaaacag cggattgaca
    30961 gtagaaaagg atttttatat cgtttataat cctgagcgaa tatatgaagg tagagcgata
    31021 gaagatattg aacaaaggta tcccggaatt gtcgctggat cgggaaaaaa gagtttagaa
    31081 attagtacaa agatattttc ttttatctat caaaaaggga taatatctat gagtaatatc
    31141 aaagctgcag aagccgaaaa actcttcgaa ggagtgtata gagatgtaaa tattgcatta
    31201 gcaaatgaat tagctatatt ttctgaacgt ttaggaatta atttttggga ggtgcgtaat
    31261 gctgcaaatt cccagccttt ttgtcatatt cacaagcccg gaattggagt aggtggggct
    31321 tgtattcctg tatatcctca atttattcta aaaactgcca aagttcttca agtggactgt
    31381 aaactaacta ggtcaagtcg attacttaat aattcaatgc caaaatattg tgttagacaa
    31441 gcactaaaat tgataaagcc aaaaaaatac aaaaatttga caataacatt attaggttta
    31501 gcatttcgag gaggcgtgtc agatactaga ttatctccaa cgtatgaagt tattaaagaa
    31561 ttacagaaac taaaagttaa agaaattagg gtgcatgacc ctctggttaa aaaagacgat
    31621 cttttatcta ggtatagaaa tacgttccta attaacgatc tggacttagc agtaaaagga
    31681 gctgacttga ttatggttat tgctgatcat aatgaatata aaaaattaaa cagtactagt
    31741 ataggaaaaa gcgcgttgta tgatggaaga ggaattacta tggaagataa gcttcatgga
    31801 gtacgccatg ctatgatagg taatttaaat tagatttcta attcaatcaa ttatattatc
    31861 taatgaaatt ctaaaactat tgtataaggt gactaaatta tctaataaac ttcttctcac
    31921 tcttgaatgg aagaataatt atcttgtttt gattgaccaa actaaactcc caactaaatt
    31981 aacatatgta agatgtagga gttacaaaga tgttgcagat gctattagaa aacttgttgt
    32041 tagaggagcg cctgcaattg gagttgctgc agcatttgga ttagccctag cagcaattaa
    32101 tagtaaaaca aataatttaa aaattttttc aaaggaatta tctaatgcat atgcaacaat
    32161 acgatcaaca cgtcctactg ctgttaacct ccaatgggct ctcgatagaa taatgcaaaa
    32221 aataatgaat ctcaaagata tacctcacat aaaaaaagtt atcctcaggg aagcacttct
    32281 aatggctaaa gaggatatag aaacgaacaa atcaatgggg ataaatggcg ctaatttatt
    32341 ttcagatggt gatactatca tgactcattg taatgctggt tcattagcta cagttgctta
    32401 cggaactgcg ttaggagtta taagagctgt taaggaatca ggtaaaaaaa ttagtgttat
    32461 tgccaccgaa accagaccag tcatgcaagg ctctagatta actgcttttg agcttcaaca
    32521 tgaaaatata gatgtaagcc ttattcctga tactgctgtt ggacacataa tgagtagcgg
    32581 tattgttaaa catgtcattg taggagcaga ccgaatatta agtactggac atgtttttaa
    32641 caaaataggt acataccagt tagcagtatt agcaaaaaca cataatattc ccttttatgt
    32701 ggtggctcct gcttctacct tcgacttgaa gaataagcca aaagatatag ttattgaaga
    32761 acgttcaaag gatgaagtta ttaggatagg taaaaaaata gtggctcctc caaacattgg
    32821 agtcattaat cctgccttcg atatgactcc acccaaatta attacaaaaa taataactga
    32881 gaagggaatt gtttcacctc cttatacgaa aagcattaaa aaaataatag ggtaattcct
    32941 aaataactct ttctaaagga ctattttcat taataacatt tttatcatct ttttccttag
    33001 gtttaaatat aaaatgtcaa aagaatcaat aactaaggat caagtttacg ttcagttaaa
    33061 aaaatgtatg gatcctgaaa ttccggtaaa tgtcgttgat ctgggactta tttataatgt
    33121 ggagattgaa gctgaaaaag atgttaatat caaaatgaca atgacaacca gaggatgtcc
    33181 tttacataat actatggtta gtgatgtaaa aaaatatgtc ggtaaaataa atggaattgg
    33241 tacgattaac gtcgaaattg tctgggatcc tccttggtct attgataaaa tgaatcctaa
    33301 tgctagagaa tccctcggat ttggaaaacc aaagctaaga tttcagataa attatgagaa
    33361 agcccttccg aaaaaagtag gctcctatat gaaatcagaa gatggttctt taattctagt
    33421 taataaagat gaaagtagtt ttatggtcaa tgatgcaata gttcgatttt gggaatcttg
    33481 tgatggcaca aaaactataa atcaacttac agagtatttt tcagaaaaat taggaatacc
    33541 catacaacaa gtagaacaag aaatagttca acttttggaa cagttatttg atgcaaagct
    33601 cattgaagtg tagtttttta ttttataata agcaaaaatt tcaaccgcta ttttgtcgct
    33661 atgtgtctca atctaatatg attttttaaa caatctgtgg agcaaaatgt atgtatgcaa
    33721 ttttctttta aacaaggaat aggttccaaa tcgttaaata ttctattaca atagatacaa
    33781 atagattcta agagagggaa ctttttagat ataaacatat ttgaacaatt tcgtttttct
    33841 ttaaaccaca ttatttccag agctaatttt tctgcatagg ttcttataat atcataggct
    33901 tcataatacc cttcagcata aaattgacga attattagtc ttggaattac accttttgta
    33961 ttattattat ttttaaatga gacgagccat ttttcggaag gtatgacttc cttttctggt
    34021 ttgttatcag atttaatttt ttttcttttt ttagtttcca ttctttataa aatgattatg
    34081 gatttttatt ttgtaaaaaa aaacttttaa tgtcgttgat gtgtatacgt ctttgctcca
    34141 tcgtatctct atatcgaata gttacagtgt tatcattaag cgtatcataa tcaattgtta
    34201 ctgcaaatgg aaccccgatt tcttctaacc tgcggtatct tctgccgata gatccagagt
    34261 catcataata tatatcaaag tcttttttca ggttagaata aatatttttt gagatttcta
    34321 ccaattctga ttttttaact aatggaagaa ttgcagccaa aatgggagca aggtaaggtt
    34381 ttaactttaa gatgactcta ttattctcgt ggtcttcata aaagcaatgc tctaaaattg
    34441 aataaatact tctatcaatt ccgagtgaaa gctcaaatat atgaggtaaa actttttcat
    34501 ttgtaactgg atccattatt tccatttttt ctttgctgat ttgagaatgt tgagataagt
    34561 cataattaga tcggtaattg catgcgacca gttctaacca gcccacactt gtttttaact
    34621 caaaatcgaa agcaactgtt gcataaaagg ctctttcttg atctgacaat ttcctaaaac
    34681 gtgtttggtt catatctata cccgtctttt cataaaattc atataaaata gataaataat
    34741 aagcgactaa tttattagga atgattttgc tgtctaacgc ttcttgtgct gtaactttag
    34801 aaactctaac accgtcatct atagttagag ttgtgttttt aatctcatca aattttgata
    34861 gagcgtctaa ttgatttgga ttacaaaata cttcgatttc agcctgataa aactctctca
    34921 atctcaatag actttgccga ggagcaatct catttctaaa actttttcct acctgcgcaa
    34981 ttggaaatgg aagttttcct ctcataattt taaaaattct aggaaagtct acaaatatag
    35041 tttgacaagt ttcaggcctt aaataagctt catctttatt aggaccaatc cctatcttaa
    35101 acatcatatt gaatcgttca ctcgcctgaa atgatgagcc acattttgga cattttaaat
    35161 tatgttgatt taatatctta tcaacctcgt gattgggtac tctttcaggc acatttattc
    35221 ctgctctatc aagaatgaat ttatcagctc taaacgtgga tttgcatttc atacatacta
    35281 ttacgggatc agaaaagttg tcgatatgtc cggaagcgac aaatacattc ttaggcatta
    35341 tttgtgatcc atcaatctca accatgtcat cccttcttac caattctcgt cgccataatt
    35401 caatgaattt atttttaatg ttgttcccat tgggaccgta ttcccaaaaa cctgcggaag
    35461 aatcagagta aatctctgag ctagggaaat agaaccctct ttctaatgag agtttcgcaa
    35521 tcctatcgta aataaccata aatattatcc atgagaataa ggctctatat ctttcttgat
    35581 attgttccat tacataatat ggttttcaat aaattttgat acaactttca tgttttcttt
    35641 atctccttga attccatcat ttggcgtttc cattattata ggaatatttt gaaaatgctt
    35701 gttttttaaa acttccatca gtccttcttt acctattgaa ccaagtccaa tatgttgatg
    35761 tctatctttc ttctcattta tttttccttt tgagtcgttc aaatgcaaaa cttttaaatt
    35821 tttaatattt attatatcgt taaaactatt cattgtatct attacttttt cagctgttct
    35881 tagatcgtat ccggaagcga atgcatgaca tgtatcaaaa caaaccccaa attttctatc
    35941 atttagtaca tccaatatat ctctaatttc ttcaaatttg ctacctatgc tatttttttg
    36001 accagaacta ttttcaatta aaatatttaa ccgcaaatgt cgtctatatg aagaatgaaa
    36061 attgtcaagt gcaaaattgc atgcattaac tatttggttt atcccattct tttcaccaaa
    36121 tccaaggtgg cttcccaagt gaagaataag aaaaggtatg tctaactcat agcatctaat
    36181 aatctcttca gcaaggacat ccttggattt attatacgat tcaccgtcag gtgctgcgaa
    36241 attaggtaaa tatggaatat gaaccacaac atcattttta tcaatataac tatcatttat
    36301 tttactcttg aattctttaa catcattcat ttctaaagac ttggctctcc aaccacgagg
    36361 attccgagta aatatttgaa aagcagtgca accaatactt tctgcatttt tgattgagtt
    36421 actaattgaa cctgtaattg atacatggaa ccccattttc aatgtattta tccaattttt
    36481 ttattcagta ccttgataat atctttacga agtagtttta ttttggtgat gattataatt
    36541 agtagtattg agcatcatat taggcaaaga tgacttggca aaatatcctt ttttagaaga
    36601 agctggaaac tatattcgag aaacacattt tgatttagat gattttaatc gatctgaatt
    36661 tttacatatt attgagagag ctacgaaaag aatcgagttt gaattaatcc aaggaaaggt
    36721 gtttattgac ttagaaagat atgatataga attatttact ttcctagttt ctctcattat
    36781 tatgaaatgt ataaatgaag actccgctat caaaaagttt tcacttttcg aagctatgcg
    36841 agtagaaaaa tttctaatat ctgatctctt aaaagaaaga gatttaacaa aaaagaacct
    36901 tcttatttct aaaattttca gggaactttt tcatttggac gtagaaatag atcctaacaa
    36961 taacaatgta tttaaaatga atatttctga ttatttacaa agagctactt cattcaacga
    37021 agaagaatgg aaattaatcg gtagagctgt ggataatggt tatgtgtatt tggatggaga
    37081 tgaaactgtc agattaataa gatatgaaat ttacaagttg atgtataata gaattaaaaa
    37141 tatgaatatt actaaaatac cagaccaaat taaacttcat gccaatttga ttaaaaataa
    37201 attccctcag cccgttaaat atacagctac tataactgat cttcccccct gtataaaaaa
    37261 agcgttggaa ttactaaata aaaatgaaaa cttacctcac tctgcaagat ttatgttagc
    37321 cac
//
LOCUS       DASVEV010000023         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1365183, whole genome shotgun sequence.
ACCESSION   DASVEV010000023 DASVEV010000000
VERSION     DASVEV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365183"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VF222_01190"
     CDS             complement(<1..1053)
                     /locus_tag="VF222_01190"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HEX7141070.1"
                     /translation="MPLSLSLMVNDKRIHILKCIILPSIFLSIIALVSTNQSLWVSSS
                     IHHFYIELFGTLLAGILSFYYISRANTLKDKFSLFIGIGFLVTTLIDLLHVTVSLLNM
                     DDILFLKYFIPQTWFAGRLFLSAMLFIAIVKYVSFSSIYPSTQQLQQQPKSSNMNTNK
                     NNENKLSRLLLLYLIIVTLFSSVLAISSLYVILPFSVIDNNPIHRPYEIFPLTLFLFS
                     LYYFYKNGIYKNKDVFYTSLAISIVFDVFGQIIMSYSAQHFDTAHNIAHLLKDASYFI
                     NIIGLALSSIQYNIGFRESNDRLRETNELLTIQYEKVKESEKMQKEFINIAAHELRNP
                     IQPILGLTEAIYSNVKD"
     gene            complement(1351..>2330)
                     /locus_tag="VF222_01195"
     CDS             complement(1351..>2330)
                     /locus_tag="VF222_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141071.1"
                     /translation="NLESIRKYYSTFINSISEYVKSYNGKVIKNIGDCLLFYFPKTTD
                     FKDMKVFKETIECGFKILDERSIVNQELSKEHLPPFNYRISMDYGVVDLALSGDYSQI
                     DLFGSTINLCSKINSSSLSIPNDITIGDNFYRILKSFSAIVNNYNFINNGEYRITESN
                     RYSTYIVKRKNNLTLNDMEISNKTSLCEKQLGSSIEDFPKKNDNKRIILVDDEQDILF
                     TYKVFLEDDDYEVTSFIDPVFALNYIRDLPNFKDLLVILDIRMKNLNGIQFYQQIKSI
                     DPTIKILFITALDILDEFSTIIPGISTKQVMKKPVDKKLFTTTVKKLLN"
BASE COUNT          885 a          288 c          302 g          855 t
ORIGIN      
        1 atctttaaca tttgaatata tagcttctgt taagccaagt ataggttgta tgggatttct
       61 cagttcatgc gcagctatat tgataaattc tttttgcatt ttttcagatt cctttacttt
      121 ctcatattga atagtaagca attcattggt ttctcttaat ctatcattgc tttctctaaa
      181 tccaatatta tattgtatgc ttgacaatgc aagtccaata atattgataa aataacttgc
      241 atcttttaaa agatgagcaa tattatgtgc tgtatcaaaa tgctgtgcag aatatgacat
      301 tattatttgt ccaaaaacat caaatacaat agaaatagct aaacttgtat agaacacatc
      361 tttattttta taaattccat tcttatagaa ataatataat gaaaataaaa ataatgttaa
      421 tggaaaaatt tcgtatggtc tatgaatagg attattatca ataacagaaa atggaaggat
      481 aacataaagt gaacttatag ctaaaacaga tgaaaataaa gtaactatta ttaggtaaag
      541 taaaagtaat cttgataatt tattttcatt attcttgtta gtattcatat tagaagattt
      601 tggttgttgt tgtagttgtt gcgtactggg atatatagag gaaaaagaaa catatttaac
      661 aattgctata aataacatag cgctaagaaa taatcttcca gcaaaccagg tctggggaat
      721 aaaatatttt aggaataaaa tatcatccat attaagaagg gaaactgtaa catgcaacaa
      781 atcaattaaa gtagtcacta gaaatccaat tccaataaat agactaaatt tgtcttttaa
      841 agtgttagca cgagaaatat agtagaatga gagtattcca gcaagcaatg taccaaataa
      901 ttctatataa aaatgatgta tggatgagct tacccataaa gattggttag tcgaaactag
      961 agcgattatt gaaaggaaaa ttgaaggtaa tataatacat tttaatatat gaatcctctt
     1021 gtcattaacc attaaagaca agctaagtgg catttaataa tgctccctgg ctttatttga
     1081 gtgaaaagaa ttaaaaataa ttttaggaaa aaaccatgga tctcatgttt tgatgatttt
     1141 ttataactat agataaattt taaattagaa atacatcttt gtgatacaac aatagtcata
     1201 ttcataactt tatattagga cctataataa attatttacc aaatttttat aatcaataaa
     1261 attatactta tatttatata agaaattaaa gaaattcaaa ctaatttagt ttcattgtaa
     1321 tataataatg tgatattgga atatttttta ttagtttaac agttttttaa cagtagttgt
     1381 aaataacttt ttatcaacag gttttttcat aacttgtttt gttgatattc ctggaattat
     1441 agttgaaaat tcatctaata tatccaaagc tgttataaaa agaattttaa tagttggatc
     1501 tatagatttt atttgctgat agaattgaat tccattcaag tttttcattc gaatatcaag
     1561 tattactaat aaatctttaa aattaggaag atctctaata taattcagag caaaaacagg
     1621 atctataaat gaagttacct cataatcatc gtcttctaaa aaaaccttgt aagtaaaaag
     1681 tatatcttgt tcatcatcga ctaaaattat ccttttgtta tcattttttt tagggaaatc
     1741 ttcaatagat gaacctaact gtttctcaca aagagaggtt ttgttgctaa tttccatatc
     1801 atttaacgtc aaattatttt ttcttttaac aatatatgta gaatatctat tactttctgt
     1861 tattctatat tcaccattat ttatgaaatt ataattgttc acaatagcag aaaaagattt
     1921 caaaattcta taaaaattgt cccctatagt tatatcatta ggaatagaca aagaagaaga
     1981 atttatcttt gaacaaaggt ttatggttga gccaaacaaa tctatttgac tataatctcc
     2041 agataaggct aaatccacta caccataatc catactaatc ctatagttga atgggggtag
     2101 atgttccttg gataattctt gattaacaat agatcgctca tctaatattt taaatccaca
     2161 ttcaatagtc tctttaaaga ctttcatatc cttaaaatca gtagttttgg gaaagtagaa
     2221 taataaacaa tcaccaatat ttttgataac ttttccgtta taacttttaa catattctga
     2281 tattgaattt ataaatgtag aataatattt tctaattgat tctaaattag
//
LOCUS       DASVEV010000024         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_5432, whole genome shotgun sequence.
ACCESSION   DASVEV010000024 DASVEV010000000
VERSION     DASVEV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5432"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..507
                     /locus_tag="VF222_01200"
     CDS             280..507
                     /locus_tag="VF222_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141072.1"
                     /translation="MTENSVRHIDTIKEMEAMKLEYQIRCSHYELEFLHGEKKITCVI
                     CGKNWLFLEERGFSKIKSAFVAEKNISALTE"
     gene            complement(512..733)
                     /locus_tag="VF222_01205"
     CDS             complement(512..733)
                     /locus_tag="VF222_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141073.1"
                     /translation="MNSNISNTDEEYVINLWKAFCRSRKALCLEYNATDAVINKWNNR
                     FLNISSIEQIRKYVKENITIEGHVLDDPF"
     gene            complement(816..1049)
                     /locus_tag="VF222_01210"
     CDS             complement(816..1049)
                     /locus_tag="VF222_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141074.1"
                     /translation="MSSLFLLIGVIWITLLVISAFLVIFIAPIDIVIFNNRTDLYITS
                     ILQATIAILIVLMLIAFLNFLKKIYIKKKLHIE"
     gene            complement(1051..2079)
                     /locus_tag="VF222_01215"
     CDS             complement(1051..2079)
                     /locus_tag="VF222_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-1-phosphate transferase"
                     /protein_id="HEX7141075.1"
                     /translation="MIFSDINSLIMVFIYGVFASAISFLTVYFAMPKAIAFLLKKGMI
                     VQDYHKPERPKIPRPAGPILILGITLTLIVIYFLTFNEKILAILFTAIIAFIIGFIDD
                     KKVMPGWFKPVALLIAALPILLLGAYDTNLNLIFGNAFIPLLYIPLILIAIPVIGNTI
                     NSIDVLNGVASGIILITGIPLMISVYIFGSVEVFLASLTLFFGTLAFYKFHKFPSKIF
                     PGDSGTLLLGAMYGAIAITGKSELIAIIALLPAIFNSFLFLSSVKRVVEHREITARPT
                     SLTDDFKLAASTDKKAPITLLRLLLVDGPLSENELVNKIFKLAIFSSILALISIVIQY
                     YFVLGGKI"
     gene            complement(2183..>2492)
                     /locus_tag="VF222_01220"
     CDS             complement(2183..>2492)
                     /locus_tag="VF222_01220"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7141076.1"
                     /translation="DMHRIAESADWLLYTLDEFAKLFNRNDILETLSDLRIRTKYGVK
                     QELLPLIKLEGVGRIRARALYNAGFVDIKILKSTAESTISSISKIGPSIAKKIKKQLT
                     "
BASE COUNT          956 a          341 c          347 g          848 t
ORIGIN      
        1 tattagtttg actttaataa atggttgaga tcataaaaag aagttatgaa gaatagtgat
       61 gaagaaaatc ttaagtcatc taataatact aaattaaaat ataaattatt catatgagaa
      121 acttttaatt atagtaatta tgcaattatt taaataataa atttttcttc aagtgaacga
      181 acacaacaat atagttcgga tattcttgat aatttataat aaagatgata gataaagttg
      241 atattaacct tactataaaa aagaattgat gaatgcacag tgacagaaaa ttctgtcaga
      301 catatcgaca caataaaaga aatggaagct atgaaattag aatatcaaat acgttgcagt
      361 cattatgagc ttgaattttt acatggtgag aaaaaaataa cttgcgttat ttgtggaaaa
      421 aattggttat ttttagagga aagaggattt tcaaaaataa aatcagcatt tgtagcagaa
      481 aaaaatattt ctgcactaac ggaataaata attagaatgg atcatctaaa acatgtccct
      541 cgattgtaat attttctttt acatattttc taatctgttc aattgaagaa atatttaaaa
      601 atcgattatt ccatttatta ataactgcat ccgtagcatt gtattctaaa caaagagcct
      661 ttctagatct acaaaaagcc ttccataaat tgataacata ttcttcatca gtatttgata
      721 tattcgagtt caatccatcc atttataaga ataagtttta atacaattga attttaattt
      781 attgaattaa taatgtaata ataaaaaata gattattatt ctatatgcaa ttttttcttg
      841 atgtaaatct tctttaaaaa attcaaaaag gctatcaaca ttaatactat caatatagct
      901 atcgttgcct gtaatattga agttatatac agatccgttc tattattaaa aattacaatg
      961 tctattggtg caataaaaat tactaaaaag gcagatatta ctaaaagtgt aatccaaata
     1021 actccaataa gcaaaaatag actggacatt ttaaattttt ccacccagga caaaataata
     1081 ttgaataaca attgaaatta atgctaaaat cgatgaaaag attgcaagtt taaagatttt
     1141 gttaactaat tcattttctg aaagtggacc gtcaactaaa agcagtctta agagtgtaat
     1201 tggagctttt ttatctgtag atgcggctag tttaaaatca tcagttaaag atgtaggcct
     1261 tgctgtaatt tctctatgtt caactaccct ttttacacta gaaaggaata gaaaggagtt
     1321 aaatattgca ggaagtaatg caataattgc aataagttct gatttgcctg ttatggctat
     1381 tgcaccatac atcgcaccta acagcaaagt accagagtct cctggaaata ttttacttgg
     1441 aaatttgtga aatttataaa aagcaagggt tccgaaaaat aaagtaagcg atgctaaaaa
     1501 aacttctacg cttccaaata tatagacact aatcattaaa ggaatacctg ttattagtat
     1561 aataccacta gctacaccat tcaaaacatc aatagaatta atagtatttc caattacagg
     1621 aatagctatt agaataagtg gtatatataa aagcggaata aaagcattac caaatataag
     1681 atttagatta gtatcatatg cccctaatag tagaataggc aaagcagcaa tcaataatgc
     1741 aacaggttta aaccaacctg gcattacttt tttatcgtct ataaatccta tgatgaaagc
     1801 aataatagca gtaaacaaga tcgcgagaat tttttcattg aatgttaaga aataaattac
     1861 tattaacgtt agtgtaattc ccagtatcag aattggacct gccggtcttg gaatctttgg
     1921 tctttcaggt ttatgataat cttgtactat catacctttt ttcagtagaa atgcaattgc
     1981 ttttggcata gcaaagtaaa cagtcaaaaa cgaaatagca gaagcaaaca ctccataaat
     2041 aaataccata ataagtgaat tgatgtcaga aaatatcata tagtatagcc actagtttgt
     2101 tttcattatt atatgtatat ttttactgaa ttatatataa aaataattct ttttattaga
     2161 tgaatagtga acattggctt gatcatgtta attgtttttt gatctttttt gcaattgatg
     2221 gcccaatttt tgatatacta gagatagttg attctgctgt tgatttcaat attttaatat
     2281 cgacaaatcc agcattatat aacgctctgg ctcgtattct tcctacacct tctaacttaa
     2341 ttagtggaag caattcttgt ttaactccat actttgttct aattcgaaga tcggataaag
     2401 tttcaagaat atcatttcta ttaaacaatt tagcaaactc atctaatgta tataataacc
     2461 aatcagctga ttctgcaatt ctatgcatat ca
//
LOCUS       DASVEV010000025        59200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1170814, whole genome shotgun sequence.
ACCESSION   DASVEV010000025 DASVEV010000000
VERSION     DASVEV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 59200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59200
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170814"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..670
                     /locus_tag="VF222_01225"
     CDS             221..670
                     /locus_tag="VF222_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141077.1"
                     /translation="MVNIDTTASLESFRKFIIVSTCKSFIPESYFRDYEVFPEREDGP
                     GAIYIEAVDKVTLKRIREMTFVNAKDILGIIYSSKSGNTQLKWRQIRKKSGKVNGTAS
                     PNALVNLVESGVLTQDWVDDYLASAQNLNKSTNQSNESTGSNSSPAV"
     gene            708..1781
                     /locus_tag="VF222_01230"
     CDS             708..1781
                     /locus_tag="VF222_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein Pelota"
                     /protein_id="HEX7141078.1"
                     /translation="MKSKEVASTSGKAMFIIPEDSDDLFTLRRIIKTDNIVVSDTSRV
                     IKFENEYSRPDRERVKIRVSVKVEKIGLDESIDRLRLAGTIIESNNSLVPKGTYHSII
                     VQIGNNIVVDKGKKWEDLDLKLLKPDVAGTFIIVAIDTKEAGIAKITGTHLEVIPNIY
                     SGQSGKYYTHGAKNNPNIEVFFEEAYLALNNIVYEDGNYKIIVFGPGETKRRFYNFII
                     GKKSGYKDMATVIDGIDVAGEDGVMVFLRSSSLKEVMSSSKISFVSAILDNIMLQVSR
                     GEEKYVMGFREVGEAIKLKSVESLVFSDSIFKDLKEEQIINMLNTAETYGANVYAVDS
                     STDIGLRVSALGGIVALLRYQIR"
     gene            complement(1857..2618)
                     /locus_tag="VF222_01235"
     CDS             complement(1857..2618)
                     /locus_tag="VF222_01235"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7141079.1"
                     /translation="MLPNNIEVSVYTDVGKVRANNEDSVYINIESKILIIADGMGGHN
                     GGEIASKLAVEIVSKYLTDRIENNILKEIEIDNYLVQALNLANFKIRIEGSHNPSLYN
                     MGTTLVLCVISETNNLHIANIGDSRLYIIDIQREKISKLTIDHTLAENQYRDGKISKE
                     YADKHPLRHILAQALGISEQINPYLTNCKWHQGEYLLLCSDGLTDMIYENDILDIFTK
                     NYKKKSDEICKTLVEIANNNGGRDNVTVIVSRRNA"
     gene            complement(2723..3058)
                     /gene="cutA"
                     /locus_tag="VF222_01240"
     CDS             complement(2723..3058)
                     /gene="cutA"
                     /locus_tag="VF222_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7141080.1"
                     /translation="MRRGRNQSAVIIISTFPNKESIIQNVNDLIIEKKLCACISLVPI
                     RSFYSWSNKLEDHEEIMVLLKTTKSLATKLKLEVARIHPYDVPEIIEVKMNDVSKSYL
                     KWMIDSIKS"
     gene            4206..5201
                     /locus_tag="VF222_01245"
     CDS             4206..5201
                     /locus_tag="VF222_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7141081.1"
                     /translation="MFSILVLTISLLGPTMDNVIKDSVKYSVIDSLSNQKVEFKDITE
                     RQTYIDQQIKLQIKNLGLEEPWYSPNRFFNTLFKVMVLDLGKSNFFVSDSGSSSVKDI
                     ILEKIPKTLLLFTTSTIVIALMGVYLGVFLASRAESKLDRTNSVFAIFSNSIPVWWFG
                     MIMIFIFAFSLNIFPARSTPLTPPDDPYYIFDLLYHMILPFITMVILGFGSVAYIVRH
                     IVSGILQEDFIKAKFAMGIPKKKILYSHALKNAAPPIVTMIALGLAGSFGGAFTVEVV
                     FDWPGMGKLYFDAIGLMDVPIIIGLTYVSTLIFLLTVFFIDIIYAYLDPRVKLNE"
     gene            5194..6798
                     /locus_tag="VF222_01250"
     CDS             5194..6798
                     /locus_tag="VF222_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7141082.1"
                     /translation="MSSKQGDNNNNNNKNNKRKHSNNETNNNNNSDSNTRKIEYATSK
                     KNLILNNNKEDEYIQNVNDNLTFIYEFIRSKSAIAGVLILLFLVSMTLYAFFGIPFAS
                     FKEWNNPNYWIDNPRLASPIWSDFFGLLERNVPEHMILSSSAGSDEDVNNSENKVIIS
                     TTNEYGVNVVTHSYHINYNSDIPPNDFMITYSLHKEEIPPAIEIEVINPNKNTFNIYF
                     DSITPSISGSNQSITLGRIFSTDNLIHQKLLDYLKFYDYKQDTSKPEVMLFSNQEKRS
                     ILKGNYTFNVKFYLFHNEDKILNSKLILGGEKFGLMGTDELRRDLTVGLVWGAPVALF
                     IGLSVSTISIVIGMIYGVIAGYKGKRTDEGLMRINDIFYSLPTLPLLIILSLFIGRSI
                     FLIVLFLIFFGWMGTAKISRSLALQIKNFQYVEAAKLIGQPDIKIIFKHIIPQLLPLT
                     FASIAISVPGAILAEASLSFIGLGDPSIPTWGQILHEAHIAAAASRGLWWWLIPPGIL
                     IALTGLAFVLIGNTIDSVLNPKMKRV"
     gene            6894..7856
                     /locus_tag="VF222_01255"
     CDS             6894..7856
                     /locus_tag="VF222_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7141083.1"
                     /translation="MNVLSEKINDYLLRPKSCNYFQKFVMLSTMIFIISGLNLIGDND
                     YVFAQVNASSPPPPSPSNNSQQTQESLGSTNRDAILSYEQGNKFLNAKNYDQALISYK
                     NAITFDPNWAAPLIKQGNTYFELANYTTAIESYDKAFAILGNLDEKVKSIDKNNGPKP
                     YWLDIWFDKGEALTNLKKFDDAIALYDNLISLDPNWANPWNSIGWALQKQGKNDEAVT
                     AFDKATKLKPTWAKPWNSKGWALYKIGKFDEALFSFDKAIALEPKWDKPWFNKGKIFY
                     DLDKYPDAKQFVEHALSLKADPKSSALLESINTKIQQSSTTTIQ"
     gene            complement(7901..8215)
                     /locus_tag="VF222_01260"
     CDS             complement(7901..8215)
                     /locus_tag="VF222_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141084.1"
                     /translation="MTLEELRDKAFYQNTIDVWIMLCNEKSVNSYDTDRYIKFIHYLN
                     SKGLKMQKFPLCIKESGGTFDRGMDKARFLDALSKVYSEDASAYTIKLSPDNISILRK
                     FF"
     gene            complement(8335..8796)
                     /locus_tag="VF222_01265"
     CDS             complement(8335..8796)
                     /locus_tag="VF222_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX7141085.1"
                     /translation="MFFNFLKNKKDGSAVTVKDKSKKTQVEYNRQVILTKQSADGIIT
                     YSKTWHPHEGILILQGKSKKNGEIRIDGLVVPPFASSGPYYSGFPTNELPMDLSYIGT
                     AHSHPGGSNRPSLQDLNNFFGVVSIIISYPYEYETIGAFDRNGNNIDLIIE"
     gene            8896..10158
                     /locus_tag="VF222_01270"
     CDS             8896..10158
                     /locus_tag="VF222_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141086.1"
                     /translation="MTKNCTMILSELGPFVFDNENELIYSKKFKNIISSNILISNGDY
                     SEILDISDKLRNYDSVFSNDTGLIFYLRNNGINVYGMPSEKIKQISKHKLNYLIQAGF
                     AENERDAHIQLRQFALDFSSSKVRRISEKLDLHISQSINALDELDKIINVIGARLREW
                     YGLHFPELDYLIQNIFTYAEIVRLAGNRNNIDLNMLENLGIESKRAEMVLVAVERSKG
                     GDILEENLSIIKKLANEVIMQTELRRILAHQIEEMMEKIAPNLKDILTATVGARLMAK
                     AGSLQKLSIMPSSTIQIIGAEKALFRSLKTGAPPPKHGILFQHPILHSAPKWQRGKMA
                     RAIASKVAIAVRIDLFRNGEKDLYISEQLNKRIAEIQEKYKEPRVGEQKPYRNEMMMN
                     YNRNPKRRMNKFTKKYAKKNKFSKRNRY"
     gene            10246..11013
                     /locus_tag="VF222_01275"
     CDS             10246..11013
                     /locus_tag="VF222_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HEX7141087.1"
                     /translation="MKNTSSIEEERSLTSPSSLSNSSSVREFNNDFDGYRWVIFNGQK
                     HLATLNLIKGNTVYDEKLITIDKEEYRLWDIFRSKLAAALVKGLKNLPIKNKTKVLYL
                     GASTGTTVSHISDIIGNSGVIFAIESSIRVARELIENVASKRENIIPIIEDARKPLSY
                     FSTYGEVDVVYCDIAQPDQTEIAIDNCKTFLKDNGKILLVVKTRSIDVTQDPRTVILQ
                     EAKKLENGGFNLEQILNLDPYDKDHGLIYATYSQIKS"
     gene            complement(10998..11666)
                     /gene="rnhB"
                     /locus_tag="VF222_01280"
     CDS             complement(10998..11666)
                     /gene="rnhB"
                     /locus_tag="VF222_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7141088.1"
                     /translation="MRNINLLICGIDEAGRGSVLGPLVIAGINIKNSDIKKLEEIGVR
                     DSKALTRKKRGFLFDKILDISEFVCIYKIDCKTIDENVYQRKLNKLEGGIMSTIIKYL
                     EADVAYVDSCDVNINRYTNYLKSNLDLKNNTSVIAMHKADRINPAVSAASIIAKVTRD
                     REIQILEENFQNIGSGYPSDKKTMYFIHNWIKEYKEFPNFVRKSWKPVREIICKQSKI
                     YDFI"
     gene            11760..12947
                     /locus_tag="VF222_01285"
     CDS             11760..12947
                     /locus_tag="VF222_01285"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: protein motif:HMM:TIGR00308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HEX7141089.1"
                     /translation="MDNFIQITEGQTKLLVPKNSLDEKVPPRYPAFFNPLARLNRDLS
                     IYIYNIFLDEYDNNKNNQITFADAFGGIGSRGLRVAVEVPKVSKIYINDINELAITAA
                     KESAHLNNVYEKCIFSINEVCNFLISRPTERNKRFTIVDLDPFGSPSPFIECVLRSVE
                     NEGLISITATDTAVLSGIYQNVCLRKYFGLSINNTYSNEVAIRLIISSTALMAARLGI
                     SLSPIFVHSNRHYFRVFLKASISNSKANMVFDNLGYIKHCFKCGERNFSRNYSKDLCP
                     ICSSGFNVAGQLWIGKLFDKIIITNLIKKHFSGDFNNDKKNQQIKQIFDISVEELDDM
                     PYYFSVDEIGSKLRTSPKKLSKIIEEIICSGYRASRTIFRPTGLKTTASMSDILYILK
                     IRE"
     gene            13259..14053
                     /locus_tag="VF222_01290"
     CDS             13259..14053
                     /locus_tag="VF222_01290"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7141090.1"
                     /translation="MVKLEIKDVTKSFYRNEGKQSTLINAIENINFAVNDGEFICLVG
                     PSGCGKSTLLNILAGLDNPTKGQVVLNGRPISGTGPDRIMVFQENALFPWMKVIDNVE
                     FGLKIAKVEKIKRREIAMQYLDMMQLKKFAESYVYQLSGGMKQRVSIARALVLDPEVL
                     LMDEPFAALDSQTRDLLLVELQLIWAKTNKTIIFVTHNIIESVCLGDRVVVFTNRPGK
                     IKKNIKIDYRRPRLTEDDNLKDFQRQILYELKSEMVHSRKEKTDLF"
     gene            complement(14045..14806)
                     /locus_tag="VF222_01295"
     CDS             complement(14045..14806)
                     /locus_tag="VF222_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141091.1"
                     /translation="MIISYELNPPKILPNNYFSYSDLSSNIENVKKKVMILDEYINCL
                     HFTDSVLGIPRLSSITMASIIKKYNAKLKISCSVRTRDRNLISMYQTISDSILLNIES
                     LLIILGDQPRNDLENQELLKPSKVVHQLNSQEFNRSIKLNLSIPNKIRNINTIKRKID
                     AKPNAFVTQSIESLTDLENIIDQVRPFDIKVVACIMVPSEKNKKSAELIGLNWKEYEK
                     EPIEFIKNSGKIADEVLITSPNHFALATEILKELK"
     gene            15014..15586
                     /locus_tag="VF222_01300"
     CDS             15014..15586
                     /locus_tag="VF222_01300"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX7141092.1"
                     /translation="MEIDKNQFIEKIIPRLSSTINVDVRLLKLSGYTLSSVLIIIHFT
                     DFTPKIILTKRSSLLKYHKNEISFPGGTYKETDSSLINTALRETKEEIGLNFHSNEIL
                     GCFQTVSTLTSNFAIIPFVTFQDSIRKTNLLIDEVSEVLDIPVFDLFRGLNNNFKYKN
                     YSDSYSFRYNSEIIWGATARILKQIRDCFY"
     gene            15671..15850
                     /locus_tag="VF222_01305"
     CDS             15671..15850
                     /locus_tag="VF222_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141093.1"
                     /translation="MQIINNDEYQMIIIKSTNNNMNPEFDSIIDTLNSVDFPILLGIF
                     GFISAVIIWGIIKNR"
     gene            15933..17387
                     /locus_tag="VF222_01310"
     CDS             15933..17387
                     /locus_tag="VF222_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX7141094.1"
                     /translation="MAFDSLSEYMDTLESVGKLHRVKTEVDTNLEIAEIMRRMMYSGQ
                     SPAILFENVKGYDIPVLGNAFGTMDLLKTALNLSDFREIGKRITDLTKLKIPSNLFDK
                     LKMLPRLSELGEYAPKYLQSGAVTEIIEMENANLNSLPILKSFPNDAGKFITFGLTIT
                     KHPETGIRNIGVYRIQIVNEKKAIMHWQTHKRGAQHYEIMKEYSKPIETAIVIGTDPS
                     TIFSGIAPVPEGMDKFFFSGIVRKKGVKLVKCKTVDLEIPANSEIVLEGYVDPNEMRI
                     EGPFGDHTGFYTPPEPYPTFNLTCMMRRRNPIYLTTVVGKPVLEDAYIGKVIEQSFLP
                     LIQMFQPEVVDFSMPASGWFQGLGVISIKKRYPGQAKKVMMGLWGMGQLSLTKIFVVV
                     DHDINVHDINEVIWAITTKSDPKRDVLIIDNTPTDTLDPASPILNLGSKMGIDATTKT
                     REEGFMREIQKQVEVDNDTKILVDKKWIHYGFHN"
     gene            17545..17928
                     /locus_tag="VF222_01315"
     CDS             17545..17928
                     /locus_tag="VF222_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7141095.1"
                     /translation="MRVITTNSNQTTSFLWEGENVLSTPSLISEMEETCRLLLKDFVL
                     KDKEWDSVGTIVDIKHLAITPVGSTIRLKAIIESVKNRRVMFNVDAYDNIEKIGEGKH
                     ERFIINVSNFRSKFEEKKKKLNVKN"
     gene            18005..19717
                     /gene="thsB"
                     /locus_tag="VF222_01320"
     CDS             18005..19717
                     /gene="thsB"
                     /locus_tag="VF222_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX7141096.1"
                     /translation="MSMGQIQTSAGGMPVVILREGTKENKGREAQKNNLTAAKLVAEV
                     VKSSLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMVVEVAKSVDNEVGDG
                     TTSSVIFTGALLEKAEELIDKNVHPSVIVDGYTAASIEALQTLDKIAIKVKTDDRDLL
                     AKIANTSMYSKLVSEDSPVLSKIVVDATQMIADSNEKTKTLKVDLDNIKVEKKAGGSM
                     QDTSLIKGIVLDKEVVHSGMPKRIEKAKIALINSPLEIEKTEMSAEIRISDPQQMQMF
                     LEEENNMLRAMVEKVKSSGANVLLCQKGIDDLAQHYLAKDGILAVRRVKESDMLKLTK
                     ATGARLVNNIDDLSSKDLGSADVVEERKVENDKWVFIEGCKNPKAVTILVRGGSQRVV
                     DEADRSLHDALMVMKDVLEKPFIVAGGGAPEAYIATHIRNWSSTLEGREQLAVIKFAE
                     ALETIPISLATNAGMDPIDTMAELRAKQNKGMKWTGVNVRSTTVSDMFKQNILEPVVI
                     KEQIIKSATEAACMLLRIDDVIASSKSKGGPPGGGMPGGMGGMGGMPGGMGGMGGMPG
                     MDMD"
     gene            complement(19875..20882)
                     /locus_tag="VF222_01325"
     CDS             complement(19875..20882)
                     /locus_tag="VF222_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141097.1"
                     /translation="MVRSFSKDPDMSKFRATRKLMTDNFESGGSSYTIKYTEHFGSSG
                     AQFQIKFNGSKIEVSINELISRSKHVTYVNLIEPILRFLFLSKEYILLHSACMSSPNG
                     QGFFLSAPPDTGKTTTVIKCVKHGYYFLSDDMTIIKLPNNAVCFPKSMTISAHTYRTA
                     VEAQSSSPNSDNDTNDKKGMRIRSLIHSKTGRSLMHKLADHNVPIFTINTIGQSRIKP
                     PKYHISDIIKDAKIQDKTTINSLFFLEKGGEGEKVEKMERISIDDALKRAIENSDDAF
                     LFPPYSDLIKYIRIEDKTAYNLLEAEKKMMEELLSSIDCYILKSSGRKWSEMILDKFS
                     T"
     gene            21294..22088
                     /locus_tag="VF222_01330"
     CDS             21294..22088
                     /locus_tag="VF222_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX7141098.1"
                     /translation="MQTNHQEIFETKNMYTISIIIPTYNESENIIRLLDKIKENLAPN
                     VFTEIIIVDDNSPDGTSNVAQNYIDGDVRPKRGNANFKEKPYSIKIINRKVKNGLIPA
                     ILEGVKSSCGDYVLIMDADFSHPPEMIPKIIEKLLDNSNSIVIASRYTKDGSIVGWPF
                     KRRLISKAAAVIAKFGLNVNNVTDPMSGFFAVPRPILENLKIDTKGYKILLEILVKSK
                     DIPIFELPYTFMDRKSGKSKMGSSVIFDYIGSVWQLYRYGRNQQKK"
     gene            22192..23715
                     /locus_tag="VF222_01335"
     CDS             22192..23715
                     /locus_tag="VF222_01335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7141099.1"
                     /translation="MTKSSGPRNKKILLIFIPLVLSSFTSLWNITGFPSLHVDEGHYM
                     RKAMSTLEGEGLQPQDRYFAPYFGQVLLAGILGIIGYPDILNPSPDADSVESLYFIPR
                     LIMGLFAILDTLLIYKITEKRYGFKIALLASILFAVIPYGWLLRRIFLESIQLPLLLT
                     SILLALYIKSNSNQEQNNNLGIRNISLILLSGVFLGLAIFTKIPIFVMMPLVGYIVMT
                     NSGNNKLKSFGLWLLPVILIPMIWPAHAYSIGDFDTWIEDVLHQTQRESKPLINSLAT
                     FLQNDPLTFSLGMAGILYAAVRRDFFILLFSIPFLLFLYWLDFVSSFHVVPLFPVFAI
                     AAARLIVDLSNLIPISKIRNTIPYFVVSVIAIVGLISIFSLITIDANSVIIKTHAVLI
                     QHLQQRNDTVTMIGNPLYIWMPRHVFDLSYQDRSYYSTRPLDTPYFIVIDDSGYKKII
                     KEESKRGNFHKNLYNQTHLVESVKPNNTLPKYKTNIYPYTNLKQSPILKQLDIRANY"
     gene            23893..25239
                     /locus_tag="VF222_01340"
     CDS             23893..25239
                     /locus_tag="VF222_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141100.1"
                     /translation="MYNNHYTTMISLILLITVVTCASLIYIDNIGEIEAQSTKKLKQE
                     PSIPILKDTNLKTELIVDKLKFPSGMEFIGNDDLLVIEKNTGKVKRVTNGEVVGDLLD
                     LNVATKSERGLLGIAVLDISKTKHASNYNPDDKFVFLFVTETENEDDGEILGNRVYRF
                     DFVNDTLINPVLLLDLPYLPGPSHNGGVMKIGPDNNLYIIMGDLNRLKDPTGDTIASN
                     LEGTSLPDGRGGVLRITPDGETVNDGFTLGEGGLLDKYYGYGVRNSFGIGFDNVTGFL
                     WDTENGSHYGDEINIIKPGFNSGWRQVLGLSSMYSEFSDKEFDKSKLMNFNGNGKYYD
                     PVLTWNDTIAPTTIAFIHSSTLGQKYMDDVLVGGVKNGTLLHFDLNQTRTGLQLEGSL
                     SDKVVNKPDELDSVIFGTGFDIITDIKIDPIDGDVYVLAANTKNGKIYKIFKVGDD"
     gene            complement(25872..26036)
                     /locus_tag="VF222_01345"
     CDS             complement(25872..26036)
                     /locus_tag="VF222_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141101.1"
                     /translation="MRESEKEKRIQKPRIYDDETLEKMKKYYNIDDSTELLRFIRSNG
                     LHNEEHYDNR"
     gene            complement(26283..28223)
                     /locus_tag="VF222_01350"
     CDS             complement(26283..28223)
                     /locus_tag="VF222_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7141102.1"
                     /translation="MFSIRSIIILGIVVFSSLLILILANSIDTSFAVDIHSPLTNNSE
                     SENNKVIKSNNNPISKQDICLRYENITKTIVVCNGTVDVPTINDFFNNNSDILEMTGE
                     KEWLLKSNILILENGTLNISGLNTQILKIDSDSSNDIAYSLISRGNLIIDNTNITSWN
                     STSNEVPELSTPETPRAYIWTFWNSAGKTNITNSFFENLGYKSHLGTTGITFFSGDGS
                     LIQNNTIINNYFGLHLANEVSNISIKNNSISRSQFDGINLASNTNNIDILENRIYENN
                     LHAIVCKKDCNNIKIKNNILENNIGIGILLERNSSINYVESNNIRSNTMGMMVAESSK
                     NDIIMNNFVGNGNGIFLKNTKNNLINSNNITNSNNYGINIYNNSTNNIIQGNNIKRSL
                     NSGVNIADFGTNNNKLINNYIAEGPNYGLKFDKTSNNILENNIIENNQNDDFFVRQSK
                     DNRIINSFFNYTEFLLNDKKSNMIITDTMNKIIDGNNITNTVYKQNNTIILNSNNNFT
                     KLKTIDMSVNPESSYVNITKVNYDLEKNKEYKRIDLVYPELGIETKFVVGGFNPGNQV
                     LVKVNKEIKDLITIDNNNSTTEFNLKNDQLSYRLEFELTTTPILITIIILSILILISI
                     GIFFLIRKKRKNREINKQQTKI"
     gene            28372..29703
                     /locus_tag="VF222_01355"
     CDS             28372..29703
                     /locus_tag="VF222_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141103.1"
                     /translation="MKLQRVVKDNTVKYLVLFFFTLLVSNTIFYTLPLQQISGGVANN
                     ATTDSGPPYNGVNMHGFYTRTSQARETNYDLPNNYFEESFKILSSNGLNHVRFLIFWE
                     SFVKNPTEFIEELKTVANTADKYNIKVLYDNHQFHTSSYLNPQRGTGFPFSLFEADFA
                     FFYGSGGGTKYPTAKVWWTNWWDRNVRDKDANDGWTLQTEFFKKVLDTVDNHTSTVGY
                     EILSEPQVHDKNQWEKIQKYNTFIADFLRKYTDKTIAYSMNIPVSLQSPIELNPINLA
                     KMAPKNVSNLVFKISVYGVPQENEYQESRLSNFVQAAELANVSLYVGEWNNVQRDKII
                     DEEGEIVTFINETFSSINQSEATKLVDVFKNLDVWGSAFWQWRVDSHQVQNFNLINIT
                     NGKIETTPYFDIVKTAYQAIYGDNSKGTSFNTNNSTLNQQEDQVNVIGTKE"
     gene            complement(29779..31698)
                     /locus_tag="VF222_01360"
     CDS             complement(29779..31698)
                     /locus_tag="VF222_01360"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7141104.1"
                     /translation="MRLLQLHADFIEYEPIEEELNDAEKDIPKSKIRLESLIVVFVSV
                     EKGDNKRTIEKAIEEVRSYSQNLKINRLLIYPYSHLSSYLASPKYAFEIIKELETNSR
                     KVLNEVFRAPFGWTKTFNIKVKGHPLAENFKVISDEQFLTEGKDKKFQKEDLKQHFLD
                     TGPVSSALKSEEKQQSLWFILDTNGVLIPLERFKIEKNSNLKRFLEYETSKRRAADEQ
                     PPHVILMKKMAIADYEPASDAGNMRYYPKGRLMKSLIEQFVTRKVLEYGGIEVETPIM
                     YDTKHPSMESYFNRFPARQYNILSDNKQLFLRFAACFGQFLMAKDFHITYKSLPLKLY
                     ELTRYSFRREKSGELVGLRRLRAFSMPDCHAFCKDIEQAKDECLKRFDLSIDVLNGFD
                     IKIEDIEMAIRFTESFYNENKDFVGQISRKFGKPILVEMWKDRFFYFILKWEFNYVDS
                     LGKAAALSTDQIDVENGKRYNIEYIDEQGNRKNPIILHNSPSGAIERIIYTLLEKAAK
                     IKQEGQRPQFPIWLSHTQVRIIPIRNEHLRIAENMENILSNSQIRTDIDDRQESLGKR
                     IREAETEWVPYILVIGDKEAESTTLVIRDRKAGKQIESNIVELIESVRNETKDKPFMP
                     LNLPKYLSKRPQIMV"
     gene            complement(31724..31933)
                     /locus_tag="VF222_01365"
     CDS             complement(31724..31933)
                     /locus_tag="VF222_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141105.1"
                     /translation="MGRVNKGINCSIKDCTEQAERSMSESKGRMANDLDISSTNKRIY
                     LCRNHYKEWKKATKEDRETERARWK"
     gene            complement(31990..33435)
                     /locus_tag="VF222_01370"
     CDS             complement(31990..33435)
                     /locus_tag="VF222_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7141106.1"
                     /translation="MDTRASEEVTIALIISVLSVLLIFSFIYVFSPNFFSKISLLEPI
                     QSLVLNISEFFGYVAINYYQLIWIYIPLGIIGLWRWSVWVFKKSTSFLYKPKVPSNDR
                     SYHKYSIITPVYNEDPKLFDQALRSWIKNEPNEIIGVIDQSDSRCIEIFTSFAKNYSF
                     MKLLIATKPGKRPALAKGIKEARNKIVALVDSDTIWAENIRKSILSPFDDQLIGGVTT
                     RQHPIERKTIWQKITDIFWDMRNYYDLPAQTAMGQALSCLSGRTSLYRKDIIMEDLDN
                     FLNEIVLGKKKESGEDKCLTRIVQSKGWKTYYQSNAVIYSYAAPDFKTFWSQRLRWSR
                     NSHNSDLISLWDGWAWKHPFLAFHIIDRFISIFTLFFGPIFFGISLYLNHYVLAVSIL
                     LLWMIGRGIKILPHLKRHPGDILLLPVYVVINFMIAIIKLYALVTIRDQKWIRSKNRK
                     SDNSTFKKIKNLILTSEIIGSLVFIVIFIIR"
     gene            33693..34565
                     /locus_tag="VF222_01375"
     CDS             33693..34565
                     /locus_tag="VF222_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7141107.1"
                     /translation="MVKISIIGSGVVGSATGKGFHKLGHNVHFYDISKNRMETLKKEG
                     FKIAHNVKDALQQSDITFVCVNTPTGDNDEQDLSQVKTALFSIKEALNESTKYNLIVF
                     RSTMLPGTMNEIVIDYLDHECNKTRGNDYDVCYNPEFLRQESAYEDFFIPDRVIIGED
                     VEGTSKLLQELYKPLTEKIIVTSPKAAEMIKYTSNCFLSLKISFFNEIGMLCKEIGID
                     DRAVSLGVSLDKRIGKYGTIAGRPFEGACLPKDTKALSTFIRKRGWTPDLLKVTLDIN
                     KKIEELVTKTALTK"
     gene            complement(34663..35727)
                     /locus_tag="VF222_01380"
     CDS             complement(34663..35727)
                     /locus_tag="VF222_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7141108.1"
                     /translation="MDPNTIFDKINDAQCNNKSITVLHDFFIDRIIKIKNIDEFFVFI
                     KEKTKYGGGSIRGIPTTQFEGGNAVNIAYCLGKLGFDVDLFTITDDSSHMNLKYFFND
                     LPKVRLHLSYGKPGYTTSLEIHNCIDLNDSNIMISDVGENSDFGPDRLSSPDDKEILN
                     NSDAVILVNWASNWKGTQLLKYVFKNSPKALHFVDPADIQTRSNEFCSLLRDPNSMID
                     ILSINENECNSLLFELDLEKYFLSKKYNRNNIELASKELAKKIGVFIDLHTRYGCACS
                     NGDEVYFAYNIKNTIVKNLTGAGDSWDAADIIGHLIKLPPQDRLFFSNAFASLYISIE
                     NRQLISLERFFEYYTNFFNN"
     gene            complement(35784..35927)
                     /locus_tag="VF222_01385"
     CDS             complement(35784..35927)
                     /locus_tag="VF222_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141109.1"
                     /translation="MRRRTAEMIMICSGIGIAAGAGGMNITYMIYGGLAILGLGVISI
                     FWR"
     gene            complement(36021..36614)
                     /gene="dps"
                     /locus_tag="VF222_01390"
     CDS             complement(36021..36614)
                     /gene="dps"
                     /locus_tag="VF222_01390"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HEX7141110.1"
                     /translation="MSNNQNSSIPKIVALEVLEKNGVNVQRLIELIRKGVGAEFTTYY
                     YYTILRMHCTGLEGEGIKEIVEDARIEDRNHFEAMTPRLYELGGSLPRDIREFADQAG
                     CPDAYLPDNWQDINSILKVLLEAEQCAIRSWGEVCDMTTGKDPRTYDIAQKIMQEEVE
                     HEAWFIELLSKRPSGHFRRKFVGQSPHVSEQGGLIHL"
     gene            36731..36871
                     /locus_tag="VF222_01395"
     CDS             36731..36871
                     /locus_tag="VF222_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141111.1"
                     /translation="MATEQIVDPFMVMIAISGFFSATILLSSLLTIRKKKIQTDKIEN
                     NI"
     gene            36890..37891
                     /locus_tag="VF222_01400"
     CDS             36890..37891
                     /locus_tag="VF222_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX7141112.1"
                     /translation="MTITICISHKEDVDGLSSAILINSAFKEKVSVVLVDYANLINKL
                     EKLLKNLIPVTKGYNRIFICDLGLNKKNQHRFISILGKMVNLGYKVIYIDHHDLEEEA
                     KKEIKKIGVNLIHSTQECTSIHVYNKYKKKLSSNASFFAAAGALTDYLDNKPVASSLI
                     PRYDRQFLMLESTAMSYMISSNQNNEEYLYNIVNVLSAMKFPHEIEGGFIEAEKYAKK
                     ISNAINSLSNEIKTKDNLALVQNNLDLASSTVVNFVLGMSEKKIAMVYKYKEEKEVFI
                     ISIRGSKDCNVHLGRIVNEIASTLGGSGGGHDKACGAVIPKDSFNNFVELLDTKTAG"
     gene            complement(38125..38484)
                     /locus_tag="VF222_01405"
     CDS             complement(38125..38484)
                     /locus_tag="VF222_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7141113.1"
                     /translation="MSEKNGNGKKTNSQLIRIEAFIREEKEDDVLVALNKLGIPATFY
                     ESKGIGKGEKYQIRYGRRGDISTMPYSNRRTVETIVTEDKVDEVVSILKDSAKIDKSG
                     GAGGIIVISKVDEIISI"
     gene            38720..39106
                     /locus_tag="VF222_01410"
     CDS             38720..39106
                     /locus_tag="VF222_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141114.1"
                     /translation="MDVFYDELSNSLSSEIPKSKNSFLKAMLDLEKFEIGESYVTSKI
                     KNKKNDTFVVNIQGGSPGIDISTSLFNLTEIPKIVYLRTMIDNMILIRKLPVLGVKDW
                     LLIYENTLFSLAVKEKYEELEILCVF"
     gene            39191..41347
                     /locus_tag="VF222_01415"
     CDS             39191..41347
                     /locus_tag="VF222_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX7141115.1"
                     /translation="MIQILEMGLAQLAKVTLILPRMESSKVASQLAQFKWFHPISSNS
                     EYSDPNLDDLHLRSQKLFQSIDEVVRELGIKTEIGIMDALIKGTPKNKKALQISEIEQ
                     LILKLEGDSKTLVNETSQIINERDSLNRQLDEYKTLKDTLSVVSNLKVDLSKLNNSKL
                     LYQNMFIIKTKDFSEIQRSLSDVSLFTLKLNDTYSALFIFANIDDSDKIGKTIRSFDI
                     SQIAIPNTFSQNPSESFLHLTTKIEELTIKKKEIDSNILKISKENNSTILYLRESSQV
                     VREILETLRKPAGLKNFAIIQGYIPTVMADSFKKLHERWITIFEHKKHNSSSQIEKDH
                     DHDSEQSKENQEPTLFANSRYFRSFEPITLTQGPPKSNEIDPTPMIAFIFPIFYGLMF
                     GDAGQGALLALLGATFRIRGTENLKKWGTLILASGIAAVIAGLLVGEAFGFHILEVPG
                     GEYLRSLGFIGILNAADFSEETVLTILTFSINIGITHLVSAFAFSIYKGIKQGKKYEV
                     LTNRLPTLIMYLSIISLMLAAVGANYKVLEMYTNQNPAPFFSNIAGDWVTVEIVTKIS
                     FLILMITMILQIIAVPLGVKLGKLKVHGNVNEELFMTLIEVMLIRLVELFANTISYTR
                     IGIMLLVHVALMGTANNGVEFFFSSGNFAVGIVVAILGNLGVMMIEGLLVYIQALRLH
                     LYEWFTKFYDGSGVPFKKLVPEMLYTSIIWEKKLQK"
     gene            41553..41858
                     /locus_tag="VF222_01420"
     CDS             41553..41858
                     /locus_tag="VF222_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141116.1"
                     /translation="MMSDFNNKNFILNQALWLGISLAISIAISLVVPFPYSLPIIIGV
                     FLLLSFYIRQRQFKKLGLSSATMFGNSSVNYYCMNCGAKHNQVSCPRCGSKMKRVGA"
     gene            complement(41907..42575)
                     /gene="tpiA"
                     /locus_tag="VF222_01425"
     CDS             complement(41907..42575)
                     /gene="tpiA"
                     /locus_tag="VF222_01425"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7141117.1"
                     /translation="MKGLIIINCKNYLEVAGEKIIQLSEIAKDISNSNNVQIMIAPPQ
                     NSLFYLSQFVKLPLICQHIDYEKIGATTGFIIPELAKSYGASGSLINHSEHRIEYEVI
                     QNLVERLRQLNMTSIVCASSSQEVGKFARLHPDMIAIEPPELIGTGKAVSKVNPSIIT
                     ESVKEAGRYSENIKVICGAGIVDKTDIKSAINLGSQGILLASGLIKSDGWKDKLIELC
                     AGFE"
     gene            complement(42621..43736)
                     /locus_tag="VF222_01430"
     CDS             complement(42621..43736)
                     /locus_tag="VF222_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEX7141118.1"
                     /translation="MRITVSAIKADIGAIGGHTRPSDELLDVVKNFIEKKGNELIIDH
                     YIGYSGDDIHIVMTHKFGVDNEKIHKLAWDAFTEGTKTAKNQGLYGAGQDLLKDSFSG
                     NVRGMGPGVAEIEMEERQSEVFCIFAADKTEPGAFNFPFWRMFVDAGSNTGLLINEKL
                     AAGVTFKIMDVMTGNIADLKMWEDKPELEAALMFPGRYVVHSVYSSEGDQILSASTDR
                     LHNIAGTYVGKDDPIAIVRTQKDFPATEEVGSAFNDPHYVSGNTRGSHHLPLMPVKLN
                     SSASLNYSIPIVECLVFSMHEGRLTGPLDGFGTADWDLQRTWASERAMIMRNQGFIHP
                     ATLVPDELEYNKGYEARMDKLNQKFTSSISNKKQKNL"
     gene            43878..45980
                     /gene="topA"
                     /locus_tag="VF222_01435"
     CDS             43878..45980
                     /gene="topA"
                     /locus_tag="VF222_01435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX7141119.1"
                     /translation="MYKSVLNLNSKDKKYSLVICEKPNVANRIAIALGTSEIKKTKIK
                     GNMVFDVLTKDNVRYVILSSKGHLYGLKDTHKRGKIFPIFDLYWSPISKPPNKNSKNL
                     IELISRFAKDTDNFIHACDYDQEGELIGYNILELICKKAYGNSHRAKLSTLTSDDINK
                     SFITLEKTNRNMAYAGMFRHLLDYIYGINFSMAVSRAIKIKNRYSYLTVGRVQTPTLK
                     FIVDRDLQINYFIPIPFWSIKAHFSKDNRVFVFFFEKTRIDSIKKVNYIIDQCSGKNG
                     IVNNINTYKEYLKPLHPFNIGDLQKESFRLFKYSPRYTLTIAENLYLKALISYPRTNS
                     QILPVSINYRKILNDLEKISDSYKRYVTILLNQNKLYPNNGIKNDPAHPAIYPTGIKP
                     INLNSAELKVYDLIVKRFFTTFGETAIFKKNKIDILVDHNFLFNLEGKNILLKGWIEF
                     YSSFYNYSESSVDYSDLKIGDTLTNIDIIKDSKFTEPPIYFNYSSLLSKMELEEIGTK
                     STRGNIIEILIQRNYVIVDNHNNIKSTDLGIFLIDFMEKNLPKIISTDLTRALELYLA
                     DIERGITDRIFILNSIITDLIDSMYRIKKIDSIMHIEHQPVRSKNYILLGQCPVCKKG
                     KFQIIRSKKTKKRFISCSNYFSSGCTASAPIPQQGSIKNTTKICKECNWPILNVFYPT
                     KRYSKNFCTNSSCPTKLI"
     gene            complement(45972..46799)
                     /locus_tag="VF222_01440"
     CDS             complement(45972..46799)
                     /locus_tag="VF222_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7141120.1"
                     /translation="MKKFLAAIVQMKSSINKQDNLIESLKYIKEAADRQADYIFFPEF
                     QMAFSSNTQTFDELYNTAETLADSKFIKRLCQYSKKYKIGIVGTFFEKNPYKTDKRVF
                     DTAFSTDKNGKIVSVYRKLHLYDAFGFKESLKFSRGDKIPKLITTPIGKMGLMICYDI
                     RFPELSRILTVNGSDIIVIPSGWVNGIMKEEHWITMLKARAMENGVYVIAPNQVGNIF
                     CGRSMIIDPLGSILTDLGDKIGVEIVGIQRERIDQIRKTLPLLKNRRTDIYNINILN"
     gene            complement(48351..48509)
                     /locus_tag="VF222_01445"
     CDS             complement(48351..48509)
                     /locus_tag="VF222_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141121.1"
                     /translation="MNFLLEEFNFLLIVNFSAVRKYKKNSMKSAAAPAAAEAAALINY
                     FRLSKRCK"
     gene            48521..49507
                     /locus_tag="VF222_01450"
     CDS             48521..49507
                     /locus_tag="VF222_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141122.1"
                     /translation="MNRHLIKNQTSKYMFKGNTKSEWRTLDATHTVIKNNNIRQNTET
                     MSIDVKNSFQQPLPLFHYDRYYFNKEEEEDEERKRKNERRFRRMLYSNFYAMHYIINN
                     SNYNNQLRSFPLYITNNRSQLTNQYFTIDTNPQMSKPDRKIPYAIKFYKCKECLNEML
                     FPIYNFKQIASIKEMNYESICQIFHKKYLLAKLGKLLKDCLFYKINTDPSHEKTSLKT
                     ITIPNLCIETPIPLIIKETLSMIYNFDDPARWLFELLTVEEFIDIGEIDSDHWIMRAY
                     NSKQGNIIALDKNELKEFFTITNASFGLIKFKRDQILRYMFSWIPFSNNPKI"
     gene            51027..53480
                     /locus_tag="VF222_01455"
     CDS             51027..53480
                     /locus_tag="VF222_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX7141123.1"
                     /translation="MHYLEDTVRMKYKINSKINKINLYYIFSLLVFFLIPVTLLNNGY
                     SQSDSVNDERDSQIDIKKKFILYINLERINSQLDLSEENVEYKNLDKAFSQAYITHTI
                     LFPNIKPDLQTIAPELSSTLEKLLTDLPIFIKDVKNKPVKEIKQTIDEIKNTNSLISN
                     KIIGNNTLNDKNFLIQSSLQLLKDASNLVSIFSSSDIVEQKNLDYDSIKGVLDRSKYQ
                     INKISSDDLRYNEKYQILSMYSKIEQNWDQKNFNALSSSITSLQSYLTPLLNANNSNT
                     NLPNEYQNYFSNITTLLDNVVVEIRENNDYKQADKHVMSAYLDNYEYLEAPIEKNDPD
                     LMVDIELDMRENLRKLIKGNEKLKVIEPFVTQIKAKLNNAKQTLENDLTIVNDSNSNS
                     NKLISKEHNLADIEELSKGFGSFQGEQKQMGESTDPQKQDVRNEIDQIRINLQHVLNL
                     YKDNKYDEALLVSRSAYLDSYENIELPLRPIDPDFTLDMEIKFAELRNMIEQRSKYEN
                     IVKKIGEIQKGLDESETLVSGIGTIAPGIAFSSSFSIIFREGLESALIIGAIITYLEA
                     SRNDRFKKHVYYGIILAAGATALTWIIAEFFIELSGASRELIEAIAGISAVAVLFWVS
                     FWVLNKIETKKWIEFVKSKVWKATTTGSVMVFVALSFFTVYREGFETVLFYQAMLSYA
                     KHMELFVILGMIIGLAVIIVVAFLVRKLGKKLPLRVLFGLTMGIGAYMSITFLGNAIR
                     EFQEVGYITTTHLFGIIPRLDVNLATMTGIHPTLETVVAQLILLSVYLVGSAYILVIQ
                     PRKKKLIESSRKSMSDFKK"
     gene            53543..53872
                     /locus_tag="VF222_01460"
     CDS             53543..53872
                     /locus_tag="VF222_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7141124.1"
                     /translation="MYSLTEIKNSWNIDKNETISFLNNILENDTYKIVLSVMENSKTV
                     YEIHEETSLPLSSIYKRIKKLEDIGIISIEKITINNKGRKLIFYKSKIKNLTFKLNEK
                     DVSLLIT"
     gene            53995..56421
                     /locus_tag="VF222_01465"
     CDS             53995..56421
                     /locus_tag="VF222_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141125.1"
                     /translation="MRLFYLFKIITPFMTYNPVGNYKKSFSLYYYDLVRSLFVTLAFT
                     GILSTGWFGIPLAPRYFTDTPINNLDVIFAIFSSLGSIWGLMAYREFTKTRDNVLLVR
                     ELCKKVDPLIGDYDYSTFNPITKKYEPGYVPLGPTDFWDKETVVPEWLDKGKYWHIML
                     RLQIKNKDIILKVVKDNDLPFFSGRRNVATTSRENLKTGSMFKRRYRYVVCVPQWLVM
                     NTEFSNFSRSKLNVNDSDFGLLMNAINKQLGLASNDITEHEKQRIKYSWRFSSLPIYL
                     IKSLPIQVIYKEIFNKTAKYIPAEKKHIIEETNVNLAYLSNDELIESLLVVARLKNIR
                     SRSIEKINILLQFLKNEYEKLGLGDGSKRYHNLHHSLEVAYVSLQMLPEELHGFYFSG
                     EDYEYILVASLLHDYDPHQYNYKTSIGLFRFEGPKVENTIRELLKIRIIDAYFSMNEV
                     EFKNYFRQYQYPLLPPVDYVTTHPEFVKPQKPLQSLIIEALIWRTDYPFKKKPHAQRN
                     YHDLIDKISNLIGSSEKYMLLSEVLSLADLSVTYMSSDPISAWYRVTSLYEELNLPRF
                     EAISRTDQFFSEIMKLELFQELLNNRNFSGVFKQRWNLVYQFYHEGNPSTQINRTIEN
                     AQSSYSKINLQIGIKTGDFLYLIALEHKDEYFIGISTDEDAVLNIKDKFANLKQQNIS
                     CFWGDAKKVLPNLPRMSIDNILFIMNNFPDFNNHNGQDPELLLLFKRASIPLKLHGTF
                     QILTNIKKNSDMEKEIIKIASNCGFQLLKSNNDCKIYFPQSYIPKEFSQHDSVSLLIF
                     HPCLNSTIVG"
     gene            56498..57568
                     /locus_tag="VF222_01470"
     CDS             56498..57568
                     /locus_tag="VF222_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7141126.1"
                     /translation="MTKKIRVAIAGVGNCASALVQGIHYYNSVNEKDCIGLTAYDLNG
                     YTPVDIEFVAAFDVSDSKVGLDLSEAIFSNPNNAIKITNVNNLDVKVNKGKVLDGVGR
                     HLAEKINVSNHPTSNISEVLKDSQTDILLNYLPVGSRAATKFYAEQCLAAGVSFINAI
                     PVFIASEPQWQQSFKEKNLLCAGDDVMSQMGATVVHKTLVKLFIDRGVKVDDTYQLNI
                     GGDMDFYNMLDEERLEDKRISKTSAVEAMSPYSIPMRIGPSDFVESLKNDKVCYINIR
                     GKKFGNIPIELDLKLRVADAYNSAGIMIDAIRATKIGIDRGLSGALESVSAYCFKHPP
                     IQMSYTQAKEKFLQFINGEQDT"
     gene            complement(57788..58558)
                     /locus_tag="VF222_01475"
     CDS             complement(57788..58558)
                     /locus_tag="VF222_01475"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7141127.1"
                     /translation="MNITDKSRKSMESLGLTSYEIKVYLSLLEHGSMTAADISKTSGV
                     PYSKIYEVLNSLENKVWLESDSSRPQTFFPKSPSTALDAIRLQKENEIKSNEEIIRNE
                     LMPIYEKSGIKEKPEIWVVRGFYNIASKVSEIIQNSQRELLIALPQIAQEIAKPLQPM
                     LRSLHDKGIRIVVLVSEGTNTEIIKTISRIADVRIKDNMFGGGVIGDGTQVLILLGEE
                     GNRKGSSEPIAIWAEHPGLAGYAKDYFNYLWNDARNNP"
     gene            59084..>59200
                     /locus_tag="VF222_01480"
     CDS             59084..>59200
                     /locus_tag="VF222_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7141128.1"
                     /translation="MPKFKSTQDIIRIIDTKDQIRNFGVIAHVDHGKTTMSDS"
BASE COUNT        20977 a         8499 c         8805 g        20919 t
ORIGIN      
        1 actatagaaa taaagaaatc tgattcagga agtatctata cataaccaat caactttaat
       61 aattttttat ctcttatatg ttgtaatgaa cttacaaata atattgtaaa tgatgtacga
      121 acagtagatt ataattgcaa cacaagataa aataatagat tttctaaaaa gtaaattttt
      181 tcataaactt gataaatgac agtatctttt taaggatata ttggttaata tcgatactac
      241 tgcatcactt gaaagtttta gaaaatttat tattgtgagt acttgtaaat cgttcattcc
      301 tgaaagttat tttcgtgatt atgaagtctt tccggaacgg gaagatggcc caggggctat
      361 atatatcgag gctgttgata aggtaacttt aaaacgaatt agagaaatga cttttgtaaa
      421 tgcaaaagat attttaggaa tcatatattc ttccaagagt ggcaacactc aattaaaatg
      481 gagacaaatc aggaaaaaat caggcaaagt taatggaacg gcatcaccta atgcattagt
      541 taatcttgta gaatcaggag tattaacgca agactgggtg gatgactatc ttgcatcggc
      601 ccaaaattta aataaatcaa cgaatcaatc aaatgaatcc acgggatcaa actcaagtcc
      661 agccgtttga tttgaaaaat ccgccatacc taataaatat ataaaatatg aaaagtaaag
      721 aggtggcaag tacttctggt aaggctatgt ttattattcc agaagattct gacgatcttt
      781 ttactttaag aaggatcatc aagacagata atatagtagt ttctgatacc tctcgagtta
      841 taaaatttga aaatgaatat tctagaccag atagagaaag agtcaaaatc cgtgtatctg
      901 ttaaagtaga gaaaataggt ctagatgaat caatagatag attgagacta gctgggacaa
      961 taattgaatc aaataatagt cttgtaccaa aaggtactta tcattcaatt atagttcaaa
     1021 ttggaaataa cattgttgtt gataaaggta aaaaatggga agatctagat ttaaaacttt
     1081 tgaaacctga tgttgccggg acttttatta ttgtagccat tgatacaaag gaagctggaa
     1141 ttgcgaaaat aactggaact catctagaag ttattccaaa tatttactca ggccaaagcg
     1201 gaaaatacta tacccatgga gccaaaaata atcccaatat agaagtattc tttgaagaag
     1261 cataccttgc attaaataat atagtatatg aagatggaaa ctataaaata atagtatttg
     1321 gccccggaga aacaaaaaga aggttttaca attttatcat aggtaaaaaa tctggttata
     1381 aagatatggc aactgttatt gatgggattg atgtggcagg cgaagatggg gtaatggtat
     1441 ttctcagatc ttcttcactc aaagaagtta tgagctctag taaaatatcc tttgtatctg
     1501 caatattaga taatatcatg ttacaggtga gtagaggtga ggaaaaatat gtgatgggat
     1561 ttagagaagt cggtgaggca ataaaattaa aatccgttga gtccctcgtt ttttctgatt
     1621 ctatatttaa agatttaaag gaagaacaaa taataaacat gctaaataca gcagaaacgt
     1681 atggagctaa tgtatatgct gttgactctt caacagatat aggattaaga gtatctgcat
     1741 taggtggcat agttgcattg cttcgatacc aaattcgtta gatagttttt ttatctctat
     1801 aaattatttg gtaatgaaat gttactttcc ttctactata aaatattgat tgaaggtcaa
     1861 gcatttctgc gactgactat gaccgtaacg ttatctctac cgccattatt gttagctatt
     1921 tctactaggg ttttacatat ctcatcagat ttttttttgt agttctttgt gaatatatct
     1981 aatatgtcgt tttcgtatat catatctgta agaccatcag aacatagaag taaatactcc
     2041 ccttgatgcc atttacaatt agttagatat ggattaattt gttcacttat acccaaagct
     2101 tgcgctaaaa tatgtctcaa cggatgtttg tctgcatatt ctttagaaat ttttccatct
     2161 ctgtattgat tttctgctaa tgtatgatct attgtcagct ttgatatctt ttctctttgg
     2221 atatcaataa tatataatct gctatctcca atatttgcaa tatgtaaatt atttgtttca
     2281 gatattacac aaagaactag tgttgttccc atattgtata acgaagggtt atgtgatcct
     2341 tctattctaa ttttaaaatt tgcaagattt aatgcttgaa ctaaatagtt atctatttct
     2401 atttctttta aaatattatt ttctattcgg tcggttaaat atttagaaac tatttctacc
     2461 gccaatttac ttgctatttc gcctccattg tgacctccca ttccatctgc aattattaaa
     2521 attttagatt ctatattgat atatacagag tcttcgttat ttgctctgac ttttccaaca
     2581 tccgtataaa cagagacttc tatgttattt gggagcacta taaattataa ttattcaaac
     2641 attttaagtt tacctaagta attagtaaat tttacttcag tatttgaaga tattaatacc
     2701 catagtataa ctattttgat atttagcttt tgattgaatc tatcatccat tttaaatatg
     2761 atttagagac atcgttcatc ttaacttcaa tgatttctgg aacatcatag gggtggattc
     2821 ttgcaacttc taattttagt ttagtagcca aagatttggt tgttttcaat aggaccatga
     2881 tctcctcatg atcttcaagc ttgttactcc aagaataaaa tgaccttata ggcactaaac
     2941 taatacatgc gcataatttt ttttctataa ttagatcatt aacattctga attattgatt
     3001 ccttatttgg gaatgtggat attattatga ctgctgactg gtttctccca cgtctcattg
     3061 gtacttaaac tataacagag tattataaga ttttagatca tttttttgat actatcttct
     3121 tattatgtgc atgttaaaag gattttgcaa attttatcaa acaataatat agaaatttaa
     3181 tagtttagtt tcattcacta aggtaaataa tttgtagttt ttttattata tctatcaaag
     3241 ttagcaataa aacagagtat caatttatac aatacatgtt aaattgctag tgtatgattg
     3301 aagaatgaaa tttattgctt tcagattgta aagtttattc tcctttggct atttactcga
     3361 ttaaggtgtt atattaaaaa actatgggtt tattaagcac ataaaattgc tattgtgtaa
     3421 ttttaagaat acaaataata atattgaata ttgggaattc acacaagatt ttaggtgaaa
     3481 agttgtttat tgtatgtatt gaaattgcag attttgttat gttatcatta acaatattgc
     3541 gtctcttcaa aataacaatt attactgtca attaaaaaat taacgcaaca aactaaaaat
     3601 taatattatt atgtatcact ataaataaat gtacattcaa gtcaatacaa atttttatca
     3661 agtctattta ctatgaatta ttttattctt atgtcacgta gtattctata ttataatagg
     3721 cttttactta gtacaacttt atatgggtta taaagtttaa aatatttgaa ggtaatataa
     3781 tttattttct aataaaataa aatattaatt agaacttatt aatcactcgt ttattcatgt
     3841 atttttaata gtgatatgtt gttaagctga gctttgggct cagggtttgc tattactagt
     3901 caatcctctg caactttctt atttagaagt agtctagatt aaaattaaac tattattatt
     3961 tgaccattta ttaatatttt aaaaatatat aaaaatacaa taaatttctt ctaagtttaa
     4021 ttaataatgc ctaaaataac ttgaaataga attattgtaa acaaatgatt tttcacaaat
     4081 tatacataag ataattgatg attaaacaat actattaata ttatttaata tcattgttct
     4141 caatataaat gacaacagga ttcttgtttt ttttatttaa aagaatcatc aatgtgttta
     4201 tagtcttgtt ttcaattctt gttttaacta tctctcttct aggtccaaca atggataatg
     4261 taattaagga ttcagtgaaa tacagtgtaa tagacagttt aagtaatcaa aaagtcgagt
     4321 ttaaggatat tacagaacgc caaacatata tcgaccaaca gattaaattg cagattaaaa
     4381 atttagggtt agaagaacca tggtattctc caaatcgatt ttttaatact ttattcaaag
     4441 ttatggtgct agatcttgga aaatcaaatt tcttcgtaag tgattcagga tcttcatctg
     4501 taaaagatat aatactggaa aaaattccta aaactctttt actttttaca acgtcaacaa
     4561 tagtaattgc tttaatgggt gtttatttag gtgttttttt ggctagtcgt gcggaatcaa
     4621 aattggatag gacaaattca gtctttgcta ttttcagtaa tagtattccc gtttggtggt
     4681 ttggaatgat aatgatattt attttcgcgt tctcactcaa tatattcccg gcccgttcaa
     4741 caccattaac tcctcccgat gatccatact atatctttga tttgctatat catatgatcc
     4801 ttccatttat tacaatggta attcttggat tcggatctgt tgcatatatt gttagacata
     4861 tagtttctgg aatattacaa gaagacttta tcaaagcgaa atttgcaatg ggtattccta
     4921 aaaaaaaaat actttattcg catgctctca aaaatgcagc ccctcctata gttacaatga
     4981 tagccctagg tctagctgga tcttttggag gagctttcac tgttgaagta gtttttgatt
     5041 ggccaggaat gggaaaatta tattttgatg ctattggatt gatggacgtt cccataatta
     5101 tcgggcttac atatgtctct acattgatat ttcttttaac agtttttttt atagatataa
     5161 tttatgcata tttggatcct agggtgaaat taaatgagta gtaaacaagg tgataacaac
     5221 aacaataaca ataaaaataa caaacgtaaa cactctaata atgaaactaa caataacaat
     5281 aattccgatt ctaataccag aaagatagaa tatgctactt ctaaaaagaa tttaatacta
     5341 aataataaca aggaggatga atatattcaa aatgtaaatg acaatttaac ttttatatat
     5401 gaatttatac gaagcaaaag cgctattgca ggagtactta ttcttttatt tttggtatct
     5461 atgaccctat atgctttttt tgggataccg tttgcatctt ttaaagaatg gaacaatcca
     5521 aattactgga tagataatcc tcgacttgca tcacctatct ggagtgattt ttttggattg
     5581 ctagaaagaa atgtaccaga acatatgatt ctttcttctt ctgctggtag tgatgaagat
     5641 gtgaataata gcgaaaataa ggtaataata tctaccacaa atgaatatgg tgttaacgtc
     5701 gttactcatt catatcatat taattataat tctgatattc caccaaatga tttcatgata
     5761 acgtattcac tacataaaga agaaattcct cctgccattg aaatagaagt aataaatcca
     5821 aacaaaaata catttaatat ttatttcgat tcaattactc catcaatctc tggttcaaat
     5881 caaagcataa cattgggaag aatattttct acagataatt taatccatca aaaactactt
     5941 gattacttga aattttatga ttataaacaa gatacttcaa aacctgaagt aatgctattt
     6001 tcaaatcagg agaaacgatc aatcctgaaa ggaaattaca ctttcaatgt taaattttat
     6061 ctcttccata acgaagacaa aattctaaat tctaaattaa ttcttggagg cgaaaaattt
     6121 ggattaatgg gcacagacga attacgtagg gatttaactg ttgggttggt gtggggagca
     6181 cctgttgctt tatttatcgg cctatctgtt tctacaattt ccattgtaat agggatgata
     6241 tatggtgtta ttgcaggata caaaggaaaa agaaccgatg aaggtttaat gagaattaat
     6301 gatatcttct attctttacc aactttgccg cttttgatta ttttgtcttt atttattgga
     6361 agaagtattt ttcttattgt tttattttta atattttttg gatggatggg tactgctaag
     6421 atttcacgaa gtctagcatt acagataaag aattttcaat atgtagaagc agcaaagctt
     6481 attggacaac cagacattaa aataatcttt aaacatatta ttcctcaact acttccttta
     6541 acttttgcaa gcattgctat ctccgttcca ggagcaattc tagccgaggc ttctcttagc
     6601 ttcattggcc taggagatcc atctattccg acatggggtc aaattcttca tgaggctcat
     6661 attgccgcag cagcctcaag aggtctatgg tggtggttaa tacctcctgg catcttaatt
     6721 gctttaacag gactagcttt tgttcttatt ggaaatacaa tagactcggt cttgaatccc
     6781 aagatgaaaa gagtgtaatt acatggacat atttctcctc cctttttttt tattgattct
     6841 tcaatataac aatcataaaa aaaaatttat gtgtgaaaat ctaataataa cgaatgaatg
     6901 tacttagtga aaagattaat gattatttat taaggccaaa gtcttgcaac tattttcaga
     6961 aatttgtcat gttaagtact atgatcttta taatttcagg attaaattta attggtgata
     7021 atgattatgt atttgcacaa gtaaatgctt cttctcctcc tcctccatct ccatccaata
     7081 atagtcaaca aacacaagaa tctctaggat caacaaatag agatgctata ttgtcttatg
     7141 aacaaggaaa taaatttttg aatgctaaaa attatgatca agctctaata tcttataaaa
     7201 atgcaattac attcgatcca aattgggctg cacctctaat taaacagggg aatacctatt
     7261 ttgaattagc caattatact acagctatag aatcatacga taaagcattt gctatattgg
     7321 gaaatttaga tgaaaaagta aaatctattg ataaaaataa tggccccaaa ccttattggc
     7381 ttgatatttg gtttgacaaa ggagaagcgc ttactaactt aaaaaaattt gatgatgcaa
     7441 tagctttata cgataattta atttccctcg atccaaattg ggctaatcct tggaatagta
     7501 taggctgggc gctgcaaaaa caaggtaaaa atgatgaggc cgtcactgca tttgataaag
     7561 caaccaaatt gaaaccaact tgggctaaac catggaatag taaaggatgg gcattataca
     7621 aaataggaaa atttgacgaa gcattattct cttttgataa agccatagct ctagaaccaa
     7681 aatgggataa accttggttt aataaaggaa aaatatttta tgatctagat aaatatccag
     7741 atgctaaaca atttgtggaa catgcattat ctttgaaagc agatcctaaa agttctgctt
     7801 tattggaaag tattaataca aaaatacaac agtcgtcaac aacaacaatt cagtaattta
     7861 agttatatat tatttattgt ggataacaaa actaaaacaa ttaaaaaaat tttctaagga
     7921 ttgaaatatt atcaggagaa agtttgatag tatatgctga tgcgtcttca gaataaacct
     7981 tagagagagc atccaaaaat ctggccttgt ccattcctct atcaaaggta ccaccagatt
     8041 ctttaataca aagaggaaat ttttgcattt tcaatccttt gctatttaaa taatgaatga
     8101 attttatata tctatcagta tcataagaat taacgctttt ttcattgcat aacattatcc
     8161 aaacatctat tgtattttga taaaaagctt tatcacgtaa ttcttcaagt gtcattatat
     8221 gtagatatta aaatatacct tttcacatat atttttactt tgttgcaatt ctaaattata
     8281 ttttagacta ccttgataat atcaagataa gattgctatt ggctacattt tactttattc
     8341 aattatcaga tcaatattat ttccattgcg atcaaacgca cctattgttt catattcgta
     8401 tggatagcta attattatag aaactactcc gaaaaaattg ttcaaatctt gtaacgatgg
     8461 tctattagaa ccgcctggat gagaatgggc tgttcctata taagaaaggt ccataggcaa
     8521 ctcgtttgtt gggaaacctg agtaataagg cccacttgac gcgaaaggcg gaactactaa
     8581 tccatcaatt cgtatttctc catttttctt gcttttacct tgcagaataa gaattccttc
     8641 gtgagggtgc catgtctttg agtatgttat aattccatct gcagattgtt ttgtaagaat
     8701 tacctgtcgg ttatattcaa cttgtgtttt tttgctttta tctttaacag taactgcaga
     8761 tccatctttt ttatttttta agaaattaaa aaacaattat gaaatatgat aataaaaatg
     8821 atatttatta atatctacca attcttcaaa cttagacata ttttttaatg gctttgttta
     8881 acaaataatg acatcatgac taaaaactgt acaatgatct tgtcagaatt aggaccattt
     8941 gtatttgaca acgaaaatga gttaatttat tcaaaaaaat tcaaaaatat tatttcttct
     9001 aatattttaa tttccaatgg tgattattct gaaattcttg atatctctga taaattacgt
     9061 aactatgatt ctgtctttag taatgataca ggtttgatat tctatcttcg aaataatgga
     9121 attaatgttt atggaatgcc ttccgaaaaa attaaacaga tttcaaaaca taaactcaat
     9181 taccttatac aggctggttt tgctgaaaat gaacgtgatg cacatataca actaagacaa
     9241 tttgctctcg atttttcttc tagtaaagtg cgacgaattt cagagaaact tgatctccat
     9301 atcagtcagt caattaatgc tcttgatgaa ctagataaaa tcataaatgt gataggagca
     9361 agacttcgtg aatggtatgg attacatttt cctgaacttg attatttaat acagaatatc
     9421 tttacctatg ctgagatcgt tagattagca ggaaatcgaa acaatatcga tttaaatatg
     9481 cttgaaaatc ttggaataga aagtaaaaga gcagaaatgg tacttgtagc tgttgaaaga
     9541 agtaaaggtg gagatatttt agaagaaaat ctgtctataa taaaaaaact tgctaatgaa
     9601 gttataatgc aaactgaatt aagaagaatt cttgcccatc aaattgaaga gatgatggaa
     9661 aaaattgctc caaacctcaa ggatatatta accgccactg ttggagcaag gttgatggct
     9721 aaagctggaa gtctccaaaa actttctatc atgccatcta gtactattca aataataggc
     9781 gctgaaaaag cactttttag atctttaaaa acaggagccc ctcctccaaa acatggaatt
     9841 ttgtttcaac atcctatatt acattcagca cctaaatggc aaagaggaaa gatggctaga
     9901 gcaattgctt ctaaggtcgc tattgcagta agaatagatc tatttaggaa tggcgaaaag
     9961 gatttgtata tttcggaaca actgaataaa cgaatagctg agatacaaga aaaatataaa
    10021 gaaccaagag ttggtgaaca aaaaccctat agaaatgaaa tgatgatgaa ttataataga
    10081 aatccaaaaa gacgaatgaa taaattcact aaaaaatatg caaagaaaaa caaattttct
    10141 aagagaaata gatactaatt taaaaatata tattgattta ctaggtaaaa gtctatgtgt
    10201 gttatattta aatatcattt aacgaatcat catttatgta tttagatgaa aaataccagc
    10261 agcattgaag aagaaagatc attaacgtca ccatcatctc tatccaactc atcttctgta
    10321 agagaattta ataatgactt tgatgggtac aggtgggtta tttttaatgg ccaaaaacat
    10381 ttagctactt tgaatttaat taagggaaat acagtatatg atgaaaaact aataacaatc
    10441 gataaggaag aatatcggct ctgggatata tttagaagta aacttgctgc agctctagtc
    10501 aaaggtttaa aaaatcttcc tataaaaaac aaaacaaaag tcctttattt aggtgcatcc
    10561 actggaacta cagtaagcca tatttcagat attataggta attctggagt aatttttgca
    10621 atagaatctt ctattcgtgt agctagagag ctcattgaaa acgttgcttc taaaagagaa
    10681 aatattattc caataataga agatgcaaga aaacctctct cctacttctc tacatatggt
    10741 gaagtggatg ttgtgtattg tgatattgct caacctgatc aaactgaaat agcaatagac
    10801 aattgtaaaa cttttcttaa agataatggt aaaatattgc tagtggtaaa aactcgaagt
    10861 atagatgtaa ctcaggatcc aagaacagtt atacttcaag aagcaaaaaa actagaaaat
    10921 ggtggattta atctagaaca aattttaaac cttgatcctt acgataaaga tcatggattg
    10981 atatatgcaa catacagtca aataaaatca taaatcttac tttgtttgca tattatttct
    11041 cttacaggtt tccatgattt tcttacaaag tttggaaatt ccttatattc tttaatccaa
    11101 ttatgtataa aatacatagt ctttttatca gagggatagc cacttccaat attttgaaaa
    11161 ttctcttcca aaatttggat ttctctatct cttgttactt ttgcaattat tgaagctgcg
    11221 gatacagcag ggtttattct gtctgcttta tgcatggcaa tgacactggt attatttttt
    11281 aaatctaaat ttgacttaag gtaatttgtg tatctattaa tatttacatc acatgaatcc
    11341 acataggcaa catctgcttc gagatattta ataattgttg acattattcc tccttctaat
    11401 ttatttaatt ttctttgata tacattttca tcaatagtct tacaatcaat tttgtatata
    11461 catacgaatt ctgaaatatc taatatttta tcaaataaaa aacctctttt ttttctggtt
    11521 aaagcttttg aatctcttac tcctatttcc tccaattttt tgatatcact atttttaata
    11581 tttataccag caattacaag aggacctagg acagatcctc ttccagcctc atcaatcccg
    11641 catattaaca aattaatatt tctcattaat tcaatctctt acattaaaat ttctatacaa
    11701 aggttaaacc aaacattagt aattatatga taaataactt ctattattct actaaaatta
    11761 tggataattt tatacagatc acagaaggtc aaacaaaatt acttgttcct aaaaattcgc
    11821 ttgacgaaaa ggttccacca cgttatcctg cctttttcaa tcctcttgca aggttaaata
    11881 gagatttgtc tatttatatt tataatattt ttttagatga atatgacaac aataaaaata
    11941 atcaaattac ctttgctgat gcatttgggg gaattggatc tagaggactc agagtagcag
    12001 tagaagtacc caaagtttcc aaaatttaca taaatgatat taatgaactg gccataacag
    12061 ctgcaaaaga atcggcacat ctaaataatg tctatgaaaa atgtattttt tcaattaatg
    12121 aggtatgtaa ttttctcatt tccaggccaa cagaaagaaa caaaagattt acaatagtag
    12181 atttggatcc attcggaagt ccttcccctt ttattgaatg cgttctcaga tctgttgaaa
    12241 atgagggatt aatttctatt acagcaactg atactgcagt tttaagtgga atatatcaaa
    12301 atgtatgcct tagaaaatat tttggtttat cgattaacaa tacctattca aatgaagttg
    12361 ccatacgctt aataatatct tcaacagcat taatggcagc aagattggga atatctttat
    12421 cccctatctt tgttcattca aatagacatt attttcgtgt atttcttaaa gcatcaataa
    12481 gtaattcaaa agctaacatg gtttttgata acctagggta cataaaacat tgttttaagt
    12541 gtggtgaaag aaatttttct cgtaattatt caaaagatct atgtccgata tgtagtagtg
    12601 gatttaacgt cgctggtcag ctctggatag gaaaattatt tgataaaatt attattacaa
    12661 acttaataaa aaaacatttt tcaggtgatt ttaataatga taaaaaaaat caacaaatta
    12721 aacaaatatt tgatattagt gtagaggaat tagatgatat gccctattac ttttcagtag
    12781 atgagatagg ctccaagctt agaaccagtc caaaaaaatt aagtaaaatt attgaggaga
    12841 ttatttgttc aggttatcgt gcatcaagga caatttttcg tcctactggc ttgaaaacta
    12901 ctgcttcaat gtctgatatt ttatatatac taaaaattag agaatgaatt aaattaaata
    12961 aataaaactc taatgctatt atttatattt gctttgtata tacattagat aaaaattgaa
    13021 tgagactaat cccatttttt atgggacaaa gccaataata gtatatatat ttaataaatt
    13081 taattccata gataataaaa gtgccaataa taaattttac caaagattct taaaggttaa
    13141 aaataacttt tttaaaagtt tttactattc cttctttata tcctagccta tggtgatttt
    13201 aaattttgtt ctttcagagt tttaagagat tggtctgtaa tggcagtggt gtattgcagt
    13261 ggttaaatta gaaataaaag atgttaccaa atcattctat agaaatgagg gaaaacaatc
    13321 aactctaatt aatgccattg aaaatattaa tttcgcagta aatgatggtg aattcatttg
    13381 tcttgtaggt ccttctggtt gtggaaagtc tactcttctc aatatattag caggactgga
    13441 taatccaaca aaaggacaag ttgtattaaa tggtaggcca attagtggaa ctggaccaga
    13501 tagaataatg gtttttcaag aaaacgctct gtttccatgg atgaaagtaa tagataatgt
    13561 agaatttgga ttaaaaatag ctaaagttga aaaaataaaa agaagagaaa tagcaatgca
    13621 ataccttgac atgatgcaat taaaaaaatt tgcagaatcg tatgtatatc aactttctgg
    13681 aggtatgaaa caaagagtct ctatagcacg agcattagtt ttggatccgg aggtattact
    13741 tatggatgaa ccatttgctg ctctagattc tcaaactcgt gatttgttat tagttgaatt
    13801 acaattaatt tgggctaaaa caaataaaac aattattttt gttacacaca acattataga
    13861 atctgtatgt cttggtgata gagtagtagt atttactaat agaccaggta aaataaaaaa
    13921 aaatattaaa atagactata gacgacctag gttaacagaa gatgataacc tcaaagattt
    13981 tcaaagacaa attctttatg aattaaaatc agagatggtt cattcaagaa aagaaaagac
    14041 agatttattt taactctttt aatatttctg ttgccaatgc aaaatgattg ggcgaagtta
    14101 ttaaaacctc atctgcaatc ttacccgaat ttttaataaa ttctataggt tctttttcat
    14161 attctttcca atttagacct attaattcag ctgatttttt gttcttttct gaaggtacca
    14221 ttatacatgc aactaccttg atatcaaaag gtctaacttg atctattatg ttttctaaat
    14281 ctgttaatga ctcgatggat tgagttacga atgcattagg ttttgcatca atttttcttt
    14341 tgatggtatt aatatttcgt attttatttg gaattgatag atttagtttt attgatctgt
    14401 tgaactcttg agagttaagc tgatgaacca ctttactagg ttttaataat tcttgattct
    14461 ctaaatcatt tctgggttga tctcctagta taatgagtaa actttcaata tttagtaaaa
    14521 tagaatcaga aatagtttga tacatagaaa tcaaatttct atctcttgtt cttacgctac
    14581 atgaaatctt caatttagcg ttatattttt ttattatgct ggccatggtt atacttgata
    14641 atctcggaat tcctaatacg gaatcggtaa aatgaagaca attgatatat tcatcaagta
    14701 tcatcacctt ttttttaaca ttttctatat tacttgaaag atctgaataa ctaaaataat
    14761 tattaggtaa gattttaggt ggatttaact cataactaat tatcaactac tttcatttaa
    14821 tttttctcta atggaatata aaatataatt gatttattta cagtattatc aaaatcgcta
    14881 tacattgtct ttccagttgt ttttatttta cccatttttt aattgtttat caattcttgt
    14941 gctcacataa aatcactttt taattatcat taaatctata attttaaaat attcctatat
    15001 ctattttaga ttattggaga tcgataaaaa ccaattcata gaaaagatta ttcctagact
    15061 ttcatctaca attaatgtcg atgtccgatt attaaaatta tctggatata ctctttcttc
    15121 tgttcttata attattcact ttacggattt cacaccaaaa attatcttaa caaaaaggag
    15181 ctctctgttg aagtatcaca aaaatgaaat ttcatttcct ggggggacat ataaagaaac
    15241 agattcctca ttaataaata ctgcactaag ggaaacaaaa gaagaaatag gtctaaattt
    15301 tcattcaaat gaaatcttgg ggtgtttcca aacagtaagt acattaactt ccaattttgc
    15361 aataattcca tttgttacat tccaggatag tattagaaaa actaatctat taatcgatga
    15421 agtctctgaa gtattggata ttcctgtatt tgatttgttt agaggtctta ataataattt
    15481 taaatataaa aattatagtg attcttactc attcagatat aatagtgaaa taatttgggg
    15541 agctaccgca agaattttaa aacaaatacg agattgtttt tattaatatt tcaacataat
    15601 ttatcgttta gttcaagttt tgattatatc acatttaaaa agagccatag ataaaaggta
    15661 aaattttttt ttgcaaataa ttaataatga tgagtatcaa atgataataa ttaaatcaac
    15721 taataataat atgaatcccg aatttgattc aataattgat actttaaatt ctgttgattt
    15781 tcctatcctt ctaggaattt ttggctttat ctctgcagta ataatttggg ggataataaa
    15841 aaaccgttaa accatgttca aacttcctaa ttctattttt tgacggaaaa gagctgtttt
    15901 tataactcta taaactagtt acttgttgat ttatggcttt tgatagttta tctgaataca
    15961 tggataccct ggaatctgtt ggcaaattac atcgtgtaaa aactgaggta gataccaatc
    16021 ttgagatagc ggagattatg agacgtatga tgtattcagg acaatcgcct gctatacttt
    16081 ttgaaaatgt aaaaggctat gatataccag tattaggtaa tgcatttgga actatggatt
    16141 tattaaaaac tgctcttaat ctctcagatt ttagagaaat tggtaaaaga attacggatt
    16201 taacaaaatt gaaaatccct tctaacctgt ttgataaatt aaagatgctt cctagattat
    16261 ctgaattagg agagtatgct ccaaagtatc ttcaatctgg tgctgtaaca gaaataattg
    16321 aaatggaaaa tgctaatctt aactctctac caattctaaa atcgtttccc aatgatgcgg
    16381 gaaaatttat tacctttgga ttaactataa ccaaacatcc agaaactgga attcgaaata
    16441 ttggtgttta tagaattcaa atcgtaaatg aaaaaaaggc tataatgcat tggcagacac
    16501 ataaacgagg agctcaacac tatgagataa tgaaggaata tagtaaacct attgaaacag
    16561 caatagtaat tggaaccgac ccatcaacca tttttagtgg aatagctcct gtaccggaag
    16621 gaatggataa atttttcttt tctggaatcg tcagaaaaaa aggggtaaaa ttagtaaaat
    16681 gcaaaacagt tgatttagaa attcctgcta actctgagat tgttttagaa ggatatgttg
    16741 accctaatga aatgagaata gaaggaccat ttggagatca tactggattt tatacacctc
    16801 ctgaaccata tccaacattc aatttgacat gtatgatgag aagaagaaat ccaatatatc
    16861 tgacaacagt tgttgggaaa ccagttttgg aagatgcata tataggtaaa gttattgaac
    16921 aatcatttct tcctcttatt caaatgtttc aacctgaggt ggttgatttt tcgatgcctg
    16981 cttccggttg gttccaagga ttgggtgtaa tatctatcaa aaaaagatat ccgggacagg
    17041 caaaaaaagt aatgatgggg ttatggggta tgggacaatt atcattaaca aaaatttttg
    17101 ttgtagttga tcatgatatt aatgtacatg atataaatga agtaatctgg gcaattacta
    17161 caaaatctga tcctaaaagg gatgtactta taattgataa tactcctaca gatacgcttg
    17221 atcctgcatc tccaattttg aatcttggtt caaaaatggg aatagacgca actactaaaa
    17281 ccagagagga aggatttatg cgagaaattc aaaaacaagt agaagttgac aatgatacca
    17341 aaattctagt tgataaaaaa tggatccatt atggcttcca taattaaatt attctattat
    17401 tcctccattt ttcattttga tttcttttca atttgttata ttcgtttaca attatagtta
    17461 ttctatcaat tagtttaata aattgaatgt gtttttaaat cttgatttta attggaattt
    17521 gatgtaaaaa ttggtgctac aaaagtgaga gttataacaa ctaattcaaa ccagactacc
    17581 agttttttat gggaaggaga aaatgttctg tctactcctt cattgatttc cgaaatggaa
    17641 gaaacatgta gactactttt aaaggatttc gttttaaaag ataaggagtg ggattcagtt
    17701 ggaaccattg ttgatattaa acatcttgct ataactccag taggttcaac aattaggttg
    17761 aaagcaataa tagaatctgt taaaaataga agagtaatgt ttaatgtaga tgcatatgat
    17821 aatattgaaa aaatcgggga aggaaaacat gaacgtttta ttattaatgt atccaatttt
    17881 agatccaaat ttgaagaaaa aaagaagaaa ttaaatgtaa agaattgata ttaagtatat
    17941 taatcgtgta aaactataaa aataggatta tttgaaatgt gtttattata ttaggtgaat
    18001 gaatatgagt atgggccaaa ttcaaacaag cgctggcgga atgccggtcg taattttaag
    18061 agaaggaaca aaggaaaata aaggaagaga ggcacaaaaa aataatctta ctgcggctaa
    18121 attggttgct gaagtagtga aatctagtct aggtccacga gggatggata aaatgttagt
    18181 tgattctcta ggagatgtta ccatcacaaa tgatggagct actatcctca aagaaataga
    18241 tgtccaacat ccagcagcaa aaatggtcgt agaagtagcc aaatcagtag ataatgaggt
    18301 cggcgatggt accacatctt cagtcatttt tactggtgcg ttattagaaa aagcagaaga
    18361 attgattgac aaaaacgttc atccatcagt tattgttgat ggttatactg ctgcttccat
    18421 tgaagctctt caaacattag ataaaatcgc tattaaagta aaaactgatg atagggatct
    18481 tttagcaaaa attgcgaata ccagtatgta ttcaaagtta gtttcagaag atagtcctgt
    18541 attaagtaaa atagtcgtag acgctacaca aatgatagcc gactcgaatg aaaaaacaaa
    18601 aactctaaaa gttgatcttg acaatattaa ggttgaaaaa aaagcaggtg gttctatgca
    18661 ggatacttct cttattaaag gaatagttct tgataaagaa gttgttcata gtggaatgcc
    18721 aaaaagaatt gaaaaagcga aaatagcatt gatcaattct ccattagaaa tagaaaaaac
    18781 tgaaatgagt gcagaaatcc gtattagtga tcctcagcaa atgcaaatgt tccttgaaga
    18841 ggaaaataat atgttaagag caatggtaga aaaagttaaa agttcaggcg caaatgtttt
    18901 actatgtcaa aaagggatag atgatcttgc acaacactac cttgctaaag atggtatact
    18961 tgcagttaga cgtgttaaag aaagtgatat gctaaaacta actaaagcca caggcgcacg
    19021 acttgtaaat aatattgatg atctttcttc gaaagacctt ggttcagccg acgtagtcga
    19081 agaacgaaag gttgaaaatg ataaatgggt tttcatagag ggatgtaaaa atccaaaggc
    19141 agtaaccatt ctggttagag gtggttcgca aagagtcgta gatgaagccg accgttctct
    19201 tcatgatgca ctgatggtta tgaaagatgt tttagaaaaa ccatttatag tagcaggtgg
    19261 aggagcacca gaagcttaca ttgctacaca tataagaaat tggtctagca ctttagaagg
    19321 cagagaacaa ttagcggtaa ttaaatttgc agaggcatta gaaaccattc caatatcact
    19381 tgctactaat gctggtatgg atccaatcga tacaatggca gagttaagag caaaacaaaa
    19441 caagggaatg aaatggactg gtgttaatgt aaggagtacc acggtttcag atatgtttaa
    19501 acaaaatata ttagagcctg ttgtaattaa agaacaaata atcaagtctg ctactgaagc
    19561 agcgtgcatg cttttacgaa ttgatgatgt tattgcatca agtaaatcta aaggaggccc
    19621 acctggaggt ggaatgcccg gcggaatggg tggaatgggc ggcatgcccg gcggaatggg
    19681 tggaatgggc ggcatgcccg gcatggatat ggattaattt ttcatttcca tctatttcca
    19741 tctttttctt tttaatagat gcgtattttc gcttaactac attagtatag aaaaaaaaca
    19801 acgataaatt attgaatact aaaataaaaa tgcatatttt ttagtatttc taaaaaagat
    19861 tagtgaattt ataattatgt agagaattta tcgagtatca tttcagacca ttttcttccg
    19921 ctacttttta ggatataaca atcaatacta gatagtaatt cctccatcat ttttttctcg
    19981 gcctctaaaa gattatatgc tgttttatct tcaattcgta tgtattttat taaatcacta
    20041 tatggtggaa atagaaatgc atcatcacta ttttctatcg ctctttttaa tgcatcatct
    20101 atagaaatcc tttccatttt ttctactttt tctccctcac cgcctttttc caaaaagaaa
    20161 agagaattga ttgttgtttt atcttgaatt ttcgcatctt ttataatgtc gctaatatgg
    20221 tattttggag gttttatcct tgactgtcca attgtattaa ttgtaaagat tggtacatta
    20281 tgatccgcca atttatgcat caaactcctt ccagtttttg agtgaattaa acttctaatt
    20341 ctcattcctt ttttatcatt tgtatcatta tcactattag gagatgatga ttgtgcttca
    20401 actgctgttc tataggtatg ggcactaatt gtcattgatt tgggaaagca tactgcatta
    20461 tttggcagtt tgattatagt catatcatct gacaaaaaat aatatccatg tttaacacat
    20521 tttattacag ttgttgtctt gccagtatct ggaggcgctg aaaggaaaaa accttgacca
    20581 tttggagagg acatacacgc agaatgtaaa agaatgtatt ccttggaaag aaagagaaat
    20641 cggagaatag gctctattaa attcacataa gtcacatgtt ttgatcttga tattaattca
    20701 ttaattgata cctctatttt gcttccatta aattttattt ggaattgagc tcctgaagaa
    20761 ccaaaatgtt ctgtatattt gatagtataa gatgaacctc cagattcaaa gttatctgtc
    20821 attaatttcc tcgtagctct aaatttagac atatcaggat ctttggaaaa agatcttacc
    20881 attagcgtaa tatctgaatc gtaaaattct ttggacgaaa aatattctaa ctcttgtaaa
    20941 tttatctctg atttaatctt aatgatatca tgaatgttat agtaatacaa atatacagaa
    21001 atactatttt taaaagagat ttaaacggta ttcctatatc ttatataaga atgttcaata
    21061 ttatatttta tagcattata ttttatagca ttttagcata aatatcaagt tagtttatga
    21121 aatttatgta atattatatt tgtggagata gcaaatgtta ataaattata taataatagt
    21181 aattatatat ggtaaaaaat attaataaat ttttgtagta aaaaaatact aaaaaatgtt
    21241 ataaatataa atcacgataa ttttaatacg cagatgtatg ttatcaaaaa taagtgcaaa
    21301 caaatcatca agaaatattt gaaacaaaga atatgtacac catttcaata attattccca
    21361 cgtataatga atcagaaaat attataagat tattagataa aattaaagaa aatttagctc
    21421 ctaatgtttt tacagaaatt ataatagtgg atgataattc tccagatggt actagtaatg
    21481 ttgcacaaaa ttacatagat ggtgatgtta gacctaaaag aggaaatgca aattttaaag
    21541 aaaagccata ttcaattaaa attattaata gaaaagtcaa aaatggttta attccagcta
    21601 tcttagaagg ggtcaaatca tcctgtggtg attatgtcct cattatggat gcagattttt
    21661 ctcatccacc tgaaatgata ccaaaaataa tagaaaaatt gctagataat tctaattcaa
    21721 tagttatagc ttctcgttat acaaaggatg gatctatagt tggatggcca tttaaaaggc
    21781 gactgataag taaagctgca gctgttattg cgaaatttgg tctaaatgta aataatgtta
    21841 cagatccaat gtcgggtttt tttgccgtgc ctagacctat tctcgaaaat ctaaaaatag
    21901 atacaaaggg ttataaaatc ctgctagaaa tacttgtaaa atcaaaagat attccaatat
    21961 tcgagctacc gtatactttc atggatagaa aatcaggtaa aagcaagatg ggatctagtg
    22021 tgatttttga ctatataggt tcagtatggc agttatatag gtatggacgc aatcaacaaa
    22081 agaaataaat ttgttgattc ttgcattatg gatacaaaga tgattattag aatcaagaaa
    22141 tcttctagtt aattctttgt catatatgat gggtgattaa ttttgaatca aatgactaaa
    22201 agtagtggtc ctaggaataa aaaaatcctt ttaattttca tacctctagt attaagttct
    22261 tttacaagtc tctggaatat aacaggtttt cctagtctcc atgtggatga gggtcattat
    22321 atgcgtaaag ctatgtctac attggaaggg gaaggtcttc aaccacaaga tagatatttt
    22381 gctccatatt ttggacaagt gttattggct ggtatcctag gaataattgg ttatcctgat
    22441 atcttaaatc catctccaga tgccgattca gtagaatcac tttattttat tccaagactc
    22501 attatgggtt tatttgctat attagatacg ttgctaatat acaaaattac agaaaaacga
    22561 tatgggttta aaatagcact cttagcatct attctatttg cagtcatacc atatgggtgg
    22621 ttattaagaa gaatattttt agagtctata caacttcctc tgctattgac ttctatacta
    22681 ttagcccttt atataaaaag taactcaaac caagaacaaa ataataatct aggaatacga
    22741 aatatatctt taattttatt atctggggta tttcttggtc tagcgatatt tacaaagatt
    22801 ccaatttttg ttatgatgcc attagttgga tatattgtaa tgactaatag tggcaacaat
    22861 aaattaaaaa gttttggact ttggctttta ccagttattt taatacctat gatatggcct
    22921 gcacacgctt attctatcgg tgattttgac acttggatag aagatgtatt acatcaaact
    22981 caaagggaga gtaaacctct aattaattct ctagctactt tccttcaaaa tgatccctta
    23041 acattttcat tggggatggc cggaattctt tatgctgcag ttagaagaga tttcttcata
    23101 ttgttattta gtataccatt tcttttgttc ctatattggc ttgactttgt atcttcattc
    23161 catgtagttc ctttatttcc tgtatttgct attgctgcag caaggttaat tgtagattta
    23221 tccaacctga tacctatctc taaaatcaga aatactattc cgtatttcgt ggtatcagtt
    23281 attgctattg ttggattaat ttcgattttt tcattaatta caattgatgc taattctgta
    23341 ataattaaga cacatgctgt attgatacaa catttacaac aaagaaatga tactgtgact
    23401 atgataggaa atcctcttta catatggatg cctagacatg tttttgatct ttcttatcaa
    23461 gatagaagtt attattctac aagaccttta gatacaccat atttcatagt aatcgatgat
    23521 tcaggttata aaaagataat taaagaagag agtaagagag gtaattttca taaaaactta
    23581 tacaatcaga ctcatctagt ggaaagtgtt aaaccaaata atactttacc aaaatacaaa
    23641 actaatattt atccatatac aaatttgaaa caatctccaa tattgaaaca acttgatata
    23701 agagcaaact actaagctat aaaaataata aataaatatt tgtccatatg tagggatata
    23761 aagataaatt ataagagaaa ataattaaat attattactt tttagttttt tgataataat
    23821 aataatagat atgtctttat aaatttttta ttggtataga ttcaaatatg atgtattcca
    23881 atgacttaat gcatgtataa taatcattac actacaatga tctctctaat attacttatt
    23941 actgttgtaa cgtgtgccag tttaatatat atagataata ttggtgaaat agaggctcag
    24001 tcgacgaaaa aattaaaaca agagccttct ataccaatac taaaagatac taatttaaaa
    24061 acagaattaa ttgttgataa attgaagttt ccttctggaa tggagtttat aggaaatgat
    24121 gatcttttag ttatagaaaa aaatacggga aaagtgaaac gtgtaactaa tggagaggtc
    24181 gttggtgatc ttttagattt aaatgtagca acaaagagtg aaagaggttt attaggtata
    24241 gctgtattgg atatatctaa aaccaaacat gctagtaatt ataatcccga tgacaaattt
    24301 gtttttcttt ttgtaactga aacagagaac gaagatgatg gggaaatttt aggaaatcgg
    24361 gtatacagat ttgattttgt gaatgacact ctaataaacc ctgtcctgct ccttgatcta
    24421 ccttatttac caggcccttc acataatgga ggagttatga aaattggccc cgataataat
    24481 ttgtatatta ttatgggtga cttaaataga ttaaaagatc caaccggtga tactattgct
    24541 tcaaatctag aggggacatc tttacctgat ggtagaggag gagtcctacg cataacacct
    24601 gatggagaaa ctgtcaatga tgggtttacc ttaggagagg gaggactttt agacaaatat
    24661 tatggatatg gtgtaagaaa tagttttgga attggttttg ataatgttac cggattttta
    24721 tgggatactg aaaatggatc tcattatggt gatgaaatta atatcatcaa acctggattt
    24781 aatagtggat ggagacaggt gttaggatta tcttctatgt atagtgaatt ttctgataag
    24841 gagtttgaca aaagtaaatt aatgaatttc aatggtaatg gtaaatatta tgatcctgta
    24901 ttaacatgga atgacacgat agccccaaca accattgcat ttattcattc ttcaacgtta
    24961 ggacaaaaat atatggatga tgtgttagta ggtggcgtaa aaaatggaac cctactacat
    25021 tttgacctta atcaaacaag aacaggattg caattagaag gatctttgtc tgataaagtg
    25081 gtaaataaac ctgatgaatt agattctgtg atttttggaa caggttttga cataataacc
    25141 gatatcaaga tagatcctat agatggagat gtttatgtct tggcagcaaa tactaagaat
    25201 ggtaaaattt ataaaatttt caaggttggt gatgattgag attctaactt atcaaaatac
    25261 tgttacttta aacacaactt ttattttatc aattataatt tttactatcc tgttacaaat
    25321 tacaaactcc ttaagtaaat ttgctattac cattaataga aaataaatca ttgaatgact
    25381 atatataaat aattaaatct taaaaaataa gacacatact tatatctgta tatctgataa
    25441 ttataaattt tgaagctcct gataatgcta tatttctctt atcaaaaacc atttagaaat
    25501 aaaaagctca gatcctagcc caataataat aaggctttta gtattattga agttaaaaat
    25561 aatattttgg aagaaggtat aaaatatttt actcattggt tatcagctga agatggaaat
    25621 agtaaaaaat gggagtattc aattgactca aatatttgaa aaagataaaa gctaataata
    25681 caggataatt ttaagaaata ttgatatcaa ggttatttga taatggtgtt ccattgtgta
    25741 ctttatgtga tagttctgaa tatgcccata gtaggtttgc aatctatttg tactgaacaa
    25801 aatcttcctc tctagtattt tagcttgtag gtacctaggt gattgaattt ttaatggttc
    25861 gattatatct tttatctgtt atcataatgt tcttcattat gtagcccatt agatctgata
    25921 aatcttaata attctgtaga atcatcaata ttataatatt ttttcatttt ttccaaggtc
    25981 tcatcatcat aaatccttgg tttttggatc cttttttctt tctcactttc tctcatacta
    26041 tttttttaca tttataatac ttaagttttt ataaattatc tataacaata gaacagtctt
    26101 actaattttt tgaaacactc ataataaaca tttatcaaag aaaaagagaa tatcactaaa
    26161 aaaaaattct tacctgaata caatactgtt tttgattata tggtatggtc tgttagattg
    26221 aaaaaattgt ttagtaaaac ttagttttat tttatcgatc acgagatgaa tcttttataa
    26281 atttaaattt tcgtttgttg tttatttatt tctctgtttt ttcttttttt tcttattaaa
    26341 aagaaaatac cgatagatat taaaattaaa atagatagga ttataatagt aattaaaatt
    26401 ggtgtagtcg ttaattcaaa ctccagtcta tatgataatt gatcattttt caaattaaat
    26461 tctgtagtac tgttattatt atcaattgtt attagatctt ttatctcttt gttcaccttt
    26521 accagtactt gatttcctgg attaaatcct cctacaacaa attttgtctc aattcctaat
    26581 tcagggtata ctaaatcaat ccttttgtat tctttatttt tttctaaatc atagtttact
    26641 tttgtgatat ttacatagga tgactctggg ttcacagaca tatcaatagt ttttaatttg
    26701 gtaaaattat tattagaatt aagtattatt gtattatttt gtttatagac tgtattagta
    26761 atattattcc catcaataat tttattcatt gtatcagtta ttatcatgtt acttttttta
    26821 tcattcagca agaattcagt ataattaaaa aaagaattta ttatcctgtt atccttggat
    26881 tgtcttacaa agaaatcatc attttgatta ttttctatta tattattttc taatatgtta
    26941 ttagatgttt tatcaaattt taagccatag ttaggtcctt cagcaatgta gttatttatc
    27001 aatttattgt tattagttcc aaaatctgca atatttactc cactattcag agaccttttt
    27061 atattattgc cttgaattat attattagta gaattattgt atatgtttat cccatagtta
    27121 ttgctgttgg ttatgttatt tgaattaatg agattattct ttgtattctt caggaaaatt
    27181 ccattaccat ttcctacaaa gttattcatt attatatcat tttttgatga ttcagcaacc
    27241 atcattccca ttgtgttgga tctaatattg tttgattcaa cataatttat gctcgaattt
    27301 ctttctaata gaattccaat accgatatta ttttctaaaa tgttattctt tatttttata
    27361 ttattacaat cttttttaca aactatggca tgtaaattgt tttcataaat tctgttttct
    27421 agtatgtcga tattgtttgt atttgaggcc aagttaatac catcaaactg gcttcttgaa
    27481 atactgttat tctttataga gatattcgaa acttcattgg ctaagtgtaa tccaaaataa
    27541 ttattaatta ttgtgttgtt ttgaattaga ctaccatcac cagaaaaaaa tgttattcct
    27601 gttgtgccta aatgactttt atatcctagg ttttcaaaaa atgaattcgt gatattagtt
    27661 tttcctgccg agttccagaa tgtccatata taagctcgtg gagtttcagg agtgcttaat
    27721 tcaggaactt cattacttgt tgaattccat gatgttatat ttgtattatc tataatcaaa
    27781 ttgccccttg aaattaaaga atacgcaata tcattggaag aatcagaatc tattttaagt
    27841 atttgagtgt tcagaccact aatatttaat gtgccgtttt caagtatcaa aatattagac
    27901 ttcaatagcc attctttttc tcctgtcatt tctagaatat ccgagttatt attaaaaaaa
    27961 tcattaatag taggaacatc tactgtccca ttacaaacta cgatagtttt tgtaatgttt
    28021 tcatatctca aacaaatatc ttgcttactg atgggattat tattactttt tataacctta
    28081 ttattttcag attcagaatt attagttaat ggactgtgaa tatcaactgc aaatgaagta
    28141 tctatactat tagctaaaat taaaattaac aaagaagaaa atacaacaat acctaaaatt
    28201 ataatacttc taatactaaa cacttgcgtt actacattaa attaatatgc tatataaatg
    28261 acgacaaaat taaaggtagt tattatctat tttttttaaa taacatactt ctagtaagaa
    28321 gggggggaag agacacacat ttatataaaa ttaatcaagt aatattggtt tatgaaatta
    28381 cagcgtgtag ttaaagataa tacagttaaa tatcttgtct tattcttttt tactttgctc
    28441 gtctccaata ccatttttta tactttacct ttacagcaaa tatctggtgg tgtcgccaat
    28501 aatgcaacca ccgatagcgg tccaccgtat aatggtgtta atatgcatgg cttttatact
    28561 agaacatctc aagctagaga aacaaattat gatcttccaa ataattattt tgaagagagt
    28621 tttaagatcc tttcctctaa tggcttaaat catgttcgat tcttgatttt ctgggaatcc
    28681 tttgtaaaaa atcctacgga attcattgaa gaattaaaga cagttgcaaa tactgctgat
    28741 aaatacaata ttaaagttct atatgataac caccagtttc atacatcgtc ttatctcaat
    28801 cctcaacgag gtactggttt tcccttttca ttatttgaag ccgattttgc ctttttctat
    28861 ggaagtggtg gtggtacgaa atatcctacc gctaaggtat ggtggacaaa ttggtgggat
    28921 agaaatgttc gtgacaaaga tgcaaacgat ggttggacct tacaaacaga attcttcaaa
    28981 aaagttctgg atactgttga taaccataca agtactgtag gctatgaaat tttaagtgaa
    29041 ccacaagtcc atgataagaa tcaatgggaa aaaatacaaa aatataatac atttatcgca
    29101 gattttctga gaaaatatac agataaaact attgcatata gtatgaatat tcctgtatct
    29161 ttacaaagtc ctattgaatt aaatccaatt aatctagcaa aaatggcacc aaagaatgtg
    29221 agtaatttag tatttaaaat aagcgtatac ggagtcccac aagaaaatga gtatcaggaa
    29281 tcaagattga gtaattttgt tcaagccgct gaactggcaa atgtttcact atacgtagga
    29341 gaatggaata atgttcagag agataaaatt attgatgaag aaggagaaat tgtcacattt
    29401 ataaatgaaa catttagcag tattaatcaa agtgaagcaa ccaaattagt agatgtgttt
    29461 aagaatcttg atgtatgggg ctctgccttt tggcaatgga gagtagattc tcatcaagta
    29521 caaaacttta acttgattaa tataaccaat ggaaagatag aaactacgcc ttattttgat
    29581 atagtaaaaa ctgcatacca ggcaatttat ggagataata gcaaaggtac ctcttttaat
    29641 actaacaata gtactctaaa ccagcaggaa gatcaagtaa atgtaattgg aacaaaagaa
    29701 tgatgataaa attcataatt ccataaatgt catatagatt ggtaagttaa taattaaatt
    29761 gaaagatgtg ataaatactt aaaccattat ttgtggtcgt ttggaaaggt acttgggcaa
    29821 atttaatggc ataaatggtt tatcttttgt ttcatttctt acagattcta ttaattctac
    29881 tatgttggat tcaatttgtt ttccggcttt acgatcacgt attaccaaag ttgtagattc
    29941 agcttcttta tctccaataa ctaatatgta tggaacccat tctgtttctg cttcccgtat
    30001 tcttttacca agagattctt gtctgtcatc tatatctgtt cttatttgac tgttagaaag
    30061 aatattctcc atattttctg caattcgcaa atgctcattc ctaataggta taatcctaac
    30121 ttgggtatgt gataaccaaa taggaaattg aggtctttga ccctcttgtt ttattttagc
    30181 agctttttct aatagtgtat aaataattct ttctatagct ccactcggtg aattatgtaa
    30241 tattattgga ttctttctgt ttccttgctc atctatatat tcaatattgt atcgttttcc
    30301 attttctaca tcgatctggt cggttgatag tgcggctgct tttcctagac tatcaacata
    30361 attaaattcc cattttaaaa taaaataaaa aaatctatcc ttccacattt caactaagat
    30421 tggttttcca aattttctag aaatctgacc aacaaaatct ttattttcat tataaaatga
    30481 ttcagtaaat cttattgcca tttctatatc ttcaattttt atatcaaatc cgtttaatac
    30541 gtctatagag agatcaaacc gttttaaaca ttcatctttc gcttgttcta tatctttgca
    30601 aaatgcatga cagtcaggca tactgaatgc tcttaatctt ctgagtccta cgagttctcc
    30661 acttttttct ctacggaaac tatatcttgt taactcataa agtttcaatg ggagactttt
    30721 atatgtaata tgaaaatcct tagccattaa aaattgacca aaacatgctg caaatcgtaa
    30781 aaataattgt ttattatctg atagtatatt atattgcctg gcaggaaagc gattgaaata
    30841 actttccata gatggatgct ttgtatcata cattatggga gtttcaactt ctattccgcc
    30901 gtattcaaga accttcctgg ttacaaactg ttctattaat gatttcatca atcttccttt
    30961 tggatagtat cgcatattac cggcatcaga agctggttca taatctgcaa tagccatttt
    31021 cttcataaga attacatgag gaggttgttc atcggcagct ctcctttttg atgtttcata
    31081 ttctaaaaac cttttcaaat tactattttt ttctattttg aatctttcta atggtattaa
    31141 tactccattt gtatctaaaa tgaaccagag agattgttgt ttttcctctg attttaaagc
    31201 agatgaaaca ggccctgtat caagaaaatg ttgttttaga tcctctttct gaaatttctt
    31261 atctttacct tcagtaagaa attgctcatc cgaaattact ttaaaatttt ccgctaatgg
    31321 gtgacctttt acttttatat tgaaggtctt agtccatcca aatggagctc taaaaacctc
    31381 gtttagtacc tttctagagt ttgtttccaa ttctttgata atttcaaaag catattttgg
    31441 agaggccaaa taagaactta agtgagaata aggataaatc aataatctat taatttttaa
    31501 attttgtgag taacttctaa cttcttctat tgctttttct atagttcttt tattatctcc
    31561 cttttctact gatacaaaaa caactattaa actttctaat ctgattttcg attttggtat
    31621 atctttttct gcatcattaa gttcctcttc aataggttca tattctatga agtctgcatg
    31681 caattgtaat aaacgcatct ttcatcttac cttgtctaat cttttatttc catctcgctc
    31741 gttctgtttc tcgatcttct tttgtagctt ttttccattc tttataatga tttctacata
    31801 aatagattct tttattagtt gaggaaatat ccaagtcatt agccattctt cctttgctct
    31861 cactcataga tctttctgct tgttcagtac agtcttttat gctacagtta atacctttat
    31921 ttactctacc catctatagt gaaattatat ttgaatataa aagctatacc gatttttggt
    31981 tttaaatttt taacgaatta tgaatattac tataaaaact aatgatccta ttatttcgct
    32041 tgttaatatt aaattcttta tctttttgaa tgtggaatta tctgatttcc tattttttga
    32101 tctaatccat ttctgatccc tgattgttac taaggcatat agttttatta tcgcaatcat
    32161 aaaattgatt actacataga ctggaagaag tagaatatct cccggatgtc tttttagatg
    32221 tggcaaaatt ttaattcctc ggcctatcat ccatagaagt aaaatggata ccgctaatac
    32281 atagtgattc aaataaaggg atataccgaa gaatattgga ccaaaaaaca atgtaaagat
    32341 ggaaataaac ctatctatta tgtgaaaagc taagaatgga tgcttccatg cccatccatc
    32401 ccacaaactt attaaatctg aattatgact atttcttgac caccttaatc gttgactcca
    32461 aaaagtctta aaatccggag cagcatatga gtatattacg gcattgctct gataatatgt
    32521 tttccaacct ttgctttgaa caattcttgt taaacattta tcctctccag attctttctt
    32581 ctttcctaat actatttcat taagaaaatt atccaaatct tccattataa tgtcttttct
    32641 atatagcgaa gttctacctg acagacacga aagggcttga cccattgcag tctgagcagg
    32701 taggtcataa taatttctca tatcccaaaa aatgtctgtt attttttgcc atattgtctt
    32761 tctttcgata ggatgttgcc ttgttgtaac gccacctatt agttgatcgt caaaaggaga
    32821 gagtatggat ttcctaatat tttctgccca tattgtgtca ctatctacaa gagcaacaat
    32881 tttatttctt gcttctttta ttcctttagc cagtgcaggt cttttaccag gcttcgttgc
    32941 gatgagaagc ttcataaatg aataattttt tgcaaaagaa gtgaaaattt caatacaacg
    33001 gctatcactt tggtctataa ccccgattat ctcattaggc tcatttttaa tccatgatct
    33061 taatgcctga tcaaaaagtt ttggatcttc attataaaca ggagtaatta tgctgtattt
    33121 gtgatagctt ctatcattag aagggacttt cggtttgtat aaaaatgagg tagatttttt
    33181 aaatacccat acactccatc tccaaagccc gataattcca agtggtatgt atatccatat
    33241 tagttgatag taattaatag ccacatagcc aaaaaattca gaaatattta aaactagaga
    33301 ttgaattggt tctaagagag atattttact aaaaaaattt ggtgagaaaa cgtatataaa
    33361 agaaaaaata agaagtactg ataagactga aataattaaa gcaatcgtaa cttcctctga
    33421 tgctcttgta tccattaatc tgtacatcta agttatttat ataatgaggc cttctaattt
    33481 atatatagat cgtaaaattt ttattaaaaa aaatattaaa gaaaaatagc aacatttgtt
    33541 tatgagataa gttttatatt taaaattcct aataatattt tatagaagaa tatttaaaag
    33601 aattcattct aactatataa gtgaaaaaga gatagtagta cttatatatt aatttgcgtt
    33661 tttttttata aaaagaggtt tgtggaacag taatggtaaa aatttctata attggatccg
    33721 gggtagttgg ttctgccacg ggaaaaggat ttcataagct aggtcacaac gttcatttct
    33781 atgatatttc taaaaatagg atggaaacgt taaagaaaga aggctttaag attgctcata
    33841 acgtcaagga tgctttgcag cagagtgata ttacatttgt ctgtgttaat acacctactg
    33901 gcgataatga tgaacaagat ttgtctcagg ttaagactgc tcttttttca ataaaagaag
    33961 cattaaatga atcgactaaa tataacttga tagtttttag aagtactatg ttgccaggaa
    34021 ctatgaatga aatagtaatt gattatctgg atcacgaatg taacaaaact agaggtaacg
    34081 attatgatgt ttgttataat ccagagtttt tacgacaaga aagtgcatat gaagattttt
    34141 ttattcctga tagagtaatt ataggagaag atgttgaagg cacttctaaa ttattacagg
    34201 aattgtacaa accccttacc gaaaaaatta ttgttactag tcctaaggct gcagagatga
    34261 tcaagtatac atctaattgc ttcttgtcat taaaaatatc attctttaat gaaatcggaa
    34321 tgttatgtaa agaaataggt atagatgata gagctgtaag tttaggcgta tccctggata
    34381 aacgtatagg taaatatgga actatcgcag gtagaccatt tgaaggtgca tgtttgccaa
    34441 aagatactaa agccttatct acatttatca gaaaaagagg atggacacca gacttactta
    34501 aagtgacttt agatataaac aaaaaaattg aagaattagt aacaaaaact gcacttacaa
    34561 aataatataa tttattttat ttactttatt aaaaaattta aatctattga tcaaatatga
    34621 attagtaata agttttttta ttttatattt ttatttgcta ttttaattat taaaaaaatt
    34681 agtgtaatat tcaaaaaatc tctctagtga aattaattgt ctgttctcta ttgatatata
    34741 taatgaagca aaagcattag aaaaaaacaa cctatcttgt gggggtagtt taataagatg
    34801 tcctataata tcagccgcat cccacgaatc gcctgcacct gttaaatttt ttacaatagt
    34861 attttttatg ttatatgcaa aatacacctc atctccatta gaacaagcac aaccatatcg
    34921 tgtatgcaag tcgataaata cccctatttt ttttgctaac tctttactag ctagttctat
    34981 attgttgcgg ttatattttt tacttaagaa atatttttcg agatctaatt caaagagtag
    35041 agaattacat tcattctcat taatacttag aatatctatc atactgtttg gatcccttag
    35101 taatgagcaa aattcattac tccgcgtttg aatatctgca ggatcaacaa aatgtaatgc
    35161 tttaggtgaa tttttaaata catattttaa aagttgagta cctttccaat tgctagccca
    35221 atttacaaga ataacagcat cagagttatt taatatttct ttatcatcgg gagaacttag
    35281 tctgtctggt ccaaaatctg aattctcacc tacatcacta atcattatat tagaatcatt
    35341 caagtctatg caattatgga tttctagtga cgttgtgtat cctggttttc catatgataa
    35401 gtgcaatctt actttaggta aatcattaaa aaaatatttt aagttcatgt gacttgaatc
    35461 atcagttata gtgaataaat ctacatcaaa tccaagcttt ccaagacaat aagcaatatt
    35521 tacggcattt cctccttcaa attgtgtggt cggtattcct ctgatgcttc cacctccata
    35581 ctttgttttt tcttttataa atacaaaaaa ttcatctata tttttaattt tgattattct
    35641 gtcaataaaa aaatcatgta atactgtaat tgatttatta ttacattgtg catcatttat
    35701 tttatcaaaa attgtgttcg gatccaaaat tgttataaaa acaccttttt gatcttttta
    35761 taacatattg ttgtttatga aagttatctc caaaatattg aaattacgcc taaccccaag
    35821 attgctaatc ctccataaat catgtaagta atattcatac ccccagcacc tgctgctatt
    35881 cctattcctg aacatatcat aatcatctct gctgttcttc gtctcattct ttaaatatta
    35941 taaaaaaagt cgcctaatta agtattatag ttcttcataa aaagattaat agataaagat
    36001 ataaaaaaag attaaagagg ttagaggtgt attaaaccgc cttgttcact aacatgtgga
    36061 gattgtccaa caaactttct tctaaagtga ccggatggtc tttttgacaa aagttcaata
    36121 aaccatgcct cgtgttcaac ttcttcttgc attatttttt gtgctatatc atatgttcgt
    36181 ggatctttac ctgtagtcat atcgcagact tctccccacg accttattgc acattgctct
    36241 gcctctagca atacctttaa tatggaattg atatcttgcc agttatcagg taaatatgca
    36301 tcaggacatc cagcttggtc agcaaattct ctaatatctc gtggtagact tccacctaat
    36361 tcgtatagtc taggagtcat tgcttcaaag tgattcctat cttcaatcct agcatcctct
    36421 actatttcct taattccctc accttctaaa cctgtacaat gcattcttaa aattgtataa
    36481 taataatatg ttgtaaactc agcgcctaca ccttttctaa ttaattctat taatctctgt
    36541 acatttacgc cattcttttc caaaacttct aatgctacaa tctttggaat ggaactgttt
    36601 tgattatttg acacaatatt cgatttgaat aataatattt aaaggtttta aaaattataa
    36661 ttaaaacagt aatcctaact aaatataggc atcttaatag tatatatata atggtttttg
    36721 gatattgtat atggcaactg aacaaattgt agatcctttt atggttatga tcgctatttc
    36781 aggatttttc tctgcaacta tcttattatc atcactttta acaattagaa agaaaaaaat
    36841 ccaaactgat aaaattgaaa ataatattta atatagaatt agcgctaaaa tgactattac
    36901 aatttgtatt tctcataaag aagatgttga tggtctatct tcagctatac tgataaattc
    36961 tgcatttaaa gaaaaagtgt ctgtagtatt agtagattat gccaatctta taaataagct
    37021 tgaaaaattg cttaaaaatc tcatacccgt aacaaaaggg tataatagaa tatttatttg
    37081 tgatcttgga ttaaacaaaa aaaatcagca cagatttatt tctatattag gtaaaatggt
    37141 taatttagga tataaagtaa tttatattga ccatcatgac cttgaagaag aagcaaagaa
    37201 ggaaataaaa aaaattggag taaatctaat tcactccact caagaatgta ccagtattca
    37261 tgtttataat aaatataaaa aaaaactttc ctccaatgca tcattttttg ctgctgctgg
    37321 tgccttaact gattatctag ataacaagcc tgtagcatca tcactgatac ctcgatatga
    37381 cagacaattt ttaatgttag aatcgacagc aatgtcttac atgatttctt ctaaccaaaa
    37441 caatgaggaa tatctgtaca atattgttaa tgtattatct gctatgaaat tccctcatga
    37501 aattgaaggt gggtttatag aagcagagaa atatgcaaaa aaaatatcta atgctattaa
    37561 ttccctttct aatgagatta aaactaaaga taatcttgct ttagtacaaa ataatcttga
    37621 tctggcgtca agtacagtag taaattttgt tctaggaatg tcagaaaaaa aaattgcgat
    37681 ggtttataaa tacaaagaag aaaaagaagt ttttattatt tctattagag gatctaaaga
    37741 ttgcaatgtt catttaggaa gaattgtaaa tgagattgca tcaactttag gaggaagtgg
    37801 aggaggtcat gataaagcat gtggagctgt aattcctaaa gattcattca ataactttgt
    37861 tgaattgtta gatacaaaaa ccgcagggta aatcggtgtc atttaataat tctaaattca
    37921 aaatatttct tttatttaaa taaattgata gatagtaaag ataaatgaat aatagaaatt
    37981 catatttgta ttattatgat attgacattt atcacttatc taaagtaaaa tttgttatta
    38041 gttgacctaa tggtttcttc ttatatttgt ataaaccaaa ttagtattat aacaaaataa
    38101 aaatgattat gaaatgttac agtattagat acttataatt tcatctactt ttgaaattac
    38161 tattattcct ccagctccac ctgatttatc aatttttgca gaatctttta gtatagacac
    38221 tacttcgtct actttatctt cagtaactat tgtttctact gttcttctat tggaataggg
    38281 cattgttgaa atatctccac gtctaccgta tctgatttga tatttttccc ctttacctat
    38341 tccctttgat tcataaaatg ttgcaggtat tccaagttta tttaatgcaa ctaaaacatc
    38401 atcttctttt tcttctctta taaaagcctc aattcttatc aattgagaat tagttttttt
    38461 tccatttcca tttttctccg acaacctagt atcaaataat attgaaagga cctttttttt
    38521 aaagttatgt tttattaaac aaataaattt ccagattaat aatataaaaa agaaaattta
    38581 taataattat aagtttttat ataaaaagta aaaaaatttt caaaggaatt taatttttta
    38641 tatttcacct gatagaatta atatgaactt tgttaaaaat taaaaaaata taatatctta
    38701 atttatatcc taaactgtta tggacgtatt ttatgatgaa ctctctaatt ctctctcgtc
    38761 tgaaattcct aaaagtaaaa attctttcct aaaagcaatg ctagatttag aaaaatttga
    38821 aatcggagaa agttacgtta cttctaagat aaaaaataaa aaaaatgata cattcgtagt
    38881 aaatattcag ggtggtagtc ctggaataga tatatctact tcacttttta atttgactga
    38941 gatccctaag attgtatatt tacgtacaat gattgataat atgattctaa ttagaaaact
    39001 acctgtactg ggagttaaag attggttact catctacgaa aatactcttt tttcgttagc
    39061 tgtaaaggaa aagtatgagg aattagaaat tctttgtgtt ttttaatttt ttaatagata
    39121 tttttgtatt ctaaaatgat tttttagcag ctatattctg aattccatag ataagatttt
    39181 aagtcgtaac atgatccaaa ttcttgagat gggcttagcg cagcttgcta aggttacttt
    39241 aattcttcct aggatggaat catcaaaagt tgctagtcag cttgcacagt ttaaatggtt
    39301 tcatccaatc agttctaatt ctgaatattc tgatcctaat ctggatgatc tacatttacg
    39361 ttcacaaaaa ctctttcaaa gtatcgatga agttgtacgt gagttaggga ttaaaacaga
    39421 aattggaatt atggatgctc ttattaaggg tacaccaaag aataaaaaag cattacaaat
    39481 ctctgaaatt gaacagttaa tattgaaact ggaaggggat tcaaagactt tggtaaatga
    39541 aacctcacaa atcattaacg aaagagattc tttaaacagg caacttgatg aatataaaac
    39601 attaaaagat actctgagtg tagtatccaa tctaaaggtc gatctatcca aacttaacaa
    39661 tagtaaatta ttatatcaaa atatgtttat tattaaaact aaagatttct cagagattca
    39721 acgttctctt tcagatgtct ctctttttac attgaaatta aatgatactt attctgcttt
    39781 attcatcttt gctaacattg atgattctga taaaatagga aaaacaatac gtagttttga
    39841 tatatcacaa attgctatac ctaatacttt ttctcaaaat ccaagcgaat ctttcctaca
    39901 tttaactaca aaaattgaag aattaactat aaagaaaaaa gaaattgatt ctaatatttt
    39961 gaagataagc aaagaaaata attcaacaat tctttatttg agagaatcct ctcaagttgt
    40021 acgtgagatt ttagaaactt taagaaaacc cgcagggtta aagaattttg ctattattca
    40081 aggatacatt ccaactgtaa tggctgatag tttcaagaag ttacatgaaa gatggataac
    40141 tatattcgag cataaaaaac ataatagttc ctctcaaata gagaaagatc atgatcatga
    40201 ttctgaacag tcaaaagaaa atcaagaacc tactttattt gcgaatagcc gttattttag
    40261 atcttttgaa ccaataacac ttacccaagg tccacctaaa tccaatgaga tagatcccac
    40321 ccctatgatt gcattcatat ttcctatttt ttacgggtta atgtttggag atgcagggca
    40381 aggtgcatta ttagccttgc ttggcgctac atttagaata agaggaacag aaaatttaaa
    40441 aaaatggggt acattaatac ttgcatcagg aatagctgca gtaattgcag gtttgctagt
    40501 aggagaggct tttggatttc atattcttga agttcccggt ggtgaatatc tgagatcatt
    40561 aggattcata ggaatattaa atgcagcaga cttttctgaa gaaactgtac ttactattct
    40621 tactttttca atcaatatag gtattaccca cctagtaagt gcttttgcat tttcaatata
    40681 taagggaata aaacaaggca agaaatatga agtacttact aaccgtcttc caactctgat
    40741 aatgtatttg tcaattattt ctctgatgtt agctgcagta ggtgctaact ataaggttct
    40801 tgaaatgtat accaatcaaa accctgcacc cttcttctca aatatagccg gtgattgggt
    40861 tactgtagaa atagttacaa aaatttcgtt cttgatactt atgataacta tgatactaca
    40921 aataattgca gttcctttgg gcgttaaact tggaaaattg aaagttcatg gaaatgtaaa
    40981 tgaagaatta tttatgacgc tgattgaagt tatgttaatt agattagtag aattatttgc
    41041 taatacaatt agttatacta gaattgggat tatgttacta gtacatgtcg ctttaatggg
    41101 gacagccaat aatggagtag aattcttttt ctccagtggt aactttgcag ttggaatagt
    41161 agtggcaata ctaggaaatc taggtgtgat gatgattgaa ggattactag tctatattca
    41221 ggcacttagg cttcatttat acgagtggtt tacaaaattc tatgatggtt caggagttcc
    41281 atttaaaaaa ttggtaccgg aaatgctata cactagcatc atttgggaaa agaaattaca
    41341 aaaataacaa caaatgatct aaataattaa aatgatatga cctaggatat ctgctcaata
    41401 tcctaggtca tgggtaaatt gttccccttc ggtttggtcg atgtattcta tatatgaata
    41461 ttaggaatat ttaaacgtga tggattagct aaaataatgt attggattct attatcaaaa
    41521 taatttataa ataacatacg agtataaatt aaatgatgag tgattttaac aataaaaatt
    41581 ttatattaaa ccaagcacta tggctaggga tatctctagc tattagtatt gctatctctc
    41641 ttgtagtacc attcccttac tcattaccaa ttataattgg agttttcctt cttctgagct
    41701 tttatataag acagagacag tttaagaaac taggcttatc aagtgccact atgtttggta
    41761 acagttctgt aaattactat tgtatgaatt gtggagctaa acataaccaa gtatcatgtc
    41821 ctcgctgcgg ttcaaaaatg aaaagagtcg gtgcctaatt ttgtatactt aaaaatttta
    41881 atcaaaataa aattgtaatg ttactattat tcaaaacctg cacagagttc tatcaattta
    41941 tctttccatc catctgactt tattaatccg cttgccagaa gtatcccttg tgatccaaga
    42001 tttattgcac ttttaatatc ggtcttatct acaattccag ctccacaaat tactttgata
    42061 ttttcagaat atctgcctgc ttctttaaca gattcagtaa ttattgatgg attgacttta
    42121 ctaactgctt ttccagttcc tattagttca ggaggttcaa tagcaatcat gtctggatgt
    42181 aatctggcaa atttaccaac ttcctgggaa gatgatgcac aaactattga agtcatattt
    42241 aattgtctca atcgttctac aaggttttga ataacttcat attcaattct gtgttcacta
    42301 tgatttatca aagaaccaga cgctccatat gattttgcta attcaggaat aataaagcca
    42361 gtagtagctc ctattttttc gtaatctata tgttgacaaa tgagaggaag tttaacgaat
    42421 tgtgataagt aaaataaaga attttgcgga ggtgctatca taatttgtac attgttacta
    42481 tttgaaatgt cttttgcaat ttctgatagt tgtatgattt tctctccagc cacttctaaa
    42541 taattcttac agttaattat aataagtcct ttcaacttat tttccttttt gtagaataat
    42601 ataatttttc ttattctctt ttaaagattt ttctgtttct tattgctaat gcttgatgta
    42661 aatttctgat ttagtttatc cattcttgct tcatatcctt tattatattc tagttcatcc
    42721 ggtactaaag ttgctggatg aatgaatcct tgatttctca taatcattgc tctttcagat
    42781 gcccatgtcc tttgtaagtc ccaatcggct gttccaaatc catcaagagg accagttagt
    42841 ctgccttcgt gcatactaaa tactaggcat tctacaatcg gtatcgaata atttaggcta
    42901 gctgatgaat tcagctttac tggcatcaaa ggcaaatgat gacttcctct tgtattacct
    42961 gagacataat gtggatcatt aaaagcacta ccaacttctt ctgttgctgg aaagtctttt
    43021 tgtgttctaa ctattgctat tggatcatcc ttaccaacat acgttccagc tatattgtgt
    43081 aatctatcag ttgatgcaga aagaatttga tctccttctg atgaataaac tgaatgaacc
    43141 acgtatctgc caggaaacat taaagcagct tctaactctg gtttatcctc ccacattttt
    43201 aaatctgcaa tattgccagt catgacatcc atgatcttaa aggttactcc agctgccaat
    43261 ttttcattta ttaaaagccc tgtattactt ccagcatcta caaacattct ccaaaatgga
    43321 aaattaaatg cgccaggctc tgtcttatca gcagcgaaaa tacagaatac ttcactttgt
    43381 ctttcttcca tttctatttc tgcaactcca ggacccatac ctctaacatt tccggaaaaa
    43441 gagtctttta acaaatcttg tcccgctcca tacaagccct gatttttagc tgtttttgtt
    43501 ccttcagtaa aggcatccca tgctagctta tgaatttttt cattatctac tccgaattta
    43561 tgggtcatga caatgtgaat atcatctccg ctatatccaa tataatggtc tatgatgagt
    43621 tcgtttccct ttttttcaat aaagttttta acaacatcaa gtaattcatc actcggcctg
    43681 gtatgtcccc caattgctcc aatatctgct ttgattgctg aaactgttat acgcataatc
    43741 tgattggatt aaatattgtt tatatctttt ctgatatgta ttttatttta attctctaaa
    43801 aaaatgggat ctacccttat tgcatttaaa actatgattt ttattataaa aattaaatat
    43861 aagaaaatta atgaaagatg tataaatccg tattgaattt gaattcaaaa gataaaaaat
    43921 attctcttgt aatttgtgaa aaacccaatg ttgctaatag aattgctatt gctttaggaa
    43981 catccgagat taaaaaaaca aaaataaaag gtaatatggt ttttgatgta cttactaaag
    44041 ataatgtacg ttatgtaatt ttatcctcca aaggacatct ttatggatta aaagatactc
    44101 ataagagagg aaaaattttt cctatttttg atttatattg gtcaccgata tctaaaccac
    44161 ccaataaaaa ttcaaaaaac ctcatagaat taataagtcg atttgctaag gatactgata
    44221 attttattca tgcatgcgat tatgatcaag aaggcgaatt aattggatat aatatattag
    44281 aattgatatg caaaaaagct tatggtaatt ctcatagagc aaaattgtct actttgactt
    44341 ctgatgatat aaataaatct tttattacat tagaaaaaac taatagaaat atggcttatg
    44401 ctggtatgtt taggcactta ttagattata tatatggaat aaatttttct atggctgtat
    44461 cacgtgctat aaaaataaaa aatagatatt cttatctaac ggtaggaaga gttcaaactc
    44521 ctactctaaa atttatagta gatagagatc ttcaaataaa ttactttatt ccaattcctt
    44581 tttggagcat aaaagctcat tttagtaaag ataatagagt ttttgttttt ttctttgaaa
    44641 aaacacgaat agactcaata aaaaaagtca actatattat agatcaatgt agtggtaaaa
    44701 atggtattgt aaataatata aatacctata aagagtatct aaaaccatta catcccttca
    44761 acataggaga tcttcaaaag gaatccttta gattatttaa atattctcca agatatactc
    44821 ttacgattgc tgagaatctt tacctaaaag cattgatttc ctatccaagg actaatagtc
    44881 aaattcttcc agtttcaatt aattatagaa aaatattaaa tgatttggaa aaaatttcag
    44941 actcatacaa aagatatgtt actatattgt taaatcaaaa taagctttat ccaaacaatg
    45001 gtataaaaaa cgatcctgct catccggcta tatatcctac tggaataaaa cctataaatc
    45061 taaattctgc cgaattaaaa gtttatgatc ttatagtaaa aagatttttt actacattcg
    45121 gcgaaactgc aatttttaaa aaaaataaaa tagatattct agttgatcat aattttttat
    45181 ttaatttaga aggaaaaaac atacttttaa aaggatggat tgaattttat agttcatttt
    45241 ataattatag tgaaagctct gttgactact ctgatcttaa aataggggat acattgacta
    45301 atatagatat tattaaagat tcaaaattca ctgaacctcc tatctatttc aattattcct
    45361 ctttattatc taaaatggag ctagaagaga taggcacaaa atctacaaga ggaaatatta
    45421 tagaaatatt aatacagaga aattatgtga ttgtagacaa ccataataat atcaaatcaa
    45481 ctgatcttgg aatattttta atagatttta tggaaaaaaa ccttcccaag ataatttcta
    45541 cagatttgac cagggcgcta gaactttatt tagctgatat tgaaagagga attacagata
    45601 gaatatttat actaaatagt ataattactg atctaattga ttctatgtat cggattaaaa
    45661 aaatagattc tataatgcat attgaacatc aaccagttag aagcaagaat tacatattgt
    45721 taggtcagtg tcctgtttgt aaaaaaggaa agtttcaaat tattagatct aaaaaaacca
    45781 aaaaacgatt tatttcatgc tcaaattatt tttccagtgg ttgtactgct tctgccccaa
    45841 ttccacaaca aggttcgatt aagaatacaa ctaaaatctg taaagaatgc aattggccaa
    45901 tactaaatgt attttatcca actaaacgat acagtaaaaa cttttgtaca aatagtagtt
    45961 gccctacaaa gttaatttaa gatattaata ttataaatat cggtccttcg attctttaat
    46021 aaaggtagtg tcttcctgat ttggtctatt ctttctcttt gaatccccac aatttctact
    46081 cctatcttat cgcctaaatc agtaagaata gatcccaaag gatctattat catacttctt
    46141 ccacaaaata tgtttcctac ttgatttggt gctattacat atactccatt ttccattgcc
    46201 cttgctttaa gcatggtaat ccaatgttct tccttcatta taccattaac ccatcctgat
    46261 ggtattacta ttatatctga accattaaca gtaagtattc tagagagttc tggaaatctt
    46321 atatcataac aaatcattaa acccatttta cctattggag ttgtgataag ttttggaatt
    46381 ttatctcctc tgctaaactt taaagattct ttaaaaccaa aagcatcata aagatgcaat
    46441 tttctataaa ctgaaacgat ttttccattt ttatcagtac tgaaagctgt atcaaaaact
    46501 cttttatctg ttttataagg atttttttca aagaatgtac ctactattcc tatcttatat
    46561 tttttagaat actgacataa tctcttaata aatttactat ctgctaatgt ttctgcagta
    46621 ttataaagtt catcaaatgt ttgagtattt gaggaaaaag ccatctgaaa ttccgggaaa
    46681 aaaatgtaat ctgcttgtct atctgctgct tctttaatgt attttaatga ttctattaaa
    46741 ttatcttgtt tgttaattga agatttcatc tgaacaatag cagccaggaa ttttttcatt
    46801 ttcaactata tgttctttaa taagaaacat taatatattt tcctattgtt gaattataaa
    46861 ttaaaattat acctatatta aatattcaat atgtggatct aatatattat aataataaaa
    46921 aatactcttg agtattctat gcctgatttc ctgaaaattt tcatttatac catctttcaa
    46981 aattattata ccctcattct acgaaagatc ttcttttatt ttcattctat ataggtacct
    47041 tccgattttc cagtggataa tacaatctta tgagaaggaa ggtttattgg agtataatta
    47101 ttattgaaaa ctgtgattac catgcacaat tttatatgga acattatagt tttataaata
    47161 ttgatattaa tttttagtag ttaaaatata aaccataaat aagactctat ttagttgata
    47221 aaattaattt aaaaatataa tgataaatag attttgtaaa tttacataat aataaataaa
    47281 tcaaataaaa aaatttatac aataataaat acaatattga ataatataat tcagagatat
    47341 cattaaactt ttcttctttt tgttacctct aaagtttttg tgcttaaatc agaggtaatg
    47401 ttattggacc aatcatcaaa tcttcttttt ctcaatctga gaggcgtttc atcattttaa
    47461 taatttaaaa aggtcaagat aaaaaatcat tttttttatg atacatgata cttaataatt
    47521 caatctacaa gaacgatata ctaaaatccc taatagtcaa attcttccag tttcaattaa
    47581 ttatagaaaa atattaaatg atttggaaaa ataataacca acatttttaa ccattatttt
    47641 tattatgtta aaacctgtat tttttagctt tttttagtta acaatactca gattttttgg
    47701 tggagatgat gatatccagc aacaaaatgc tacacttgct ttaacagatt ctagttttag
    47761 agcaagagga gactggtact gtaatgagga aacacaaaac actataaata atattcttga
    47821 tgcccatcct gaactaataa ttacaactgg agatcaggtc aaagaatcgc catcggttga
    47881 atgctggatg gatatgtcaa aacctattaa agataaatga aaattgcaat atgaaatctt
    47941 aatactgaat tttcaaatat atataaacag attgttgact atcaccaact gaagaatcca
    48001 tattattcac atgacttgaa aaatttcact ttataagttt gtctactgaa tatccttatg
    48061 aggaaggaag caaacagtat gcgtttactc gaactgattt aggaaaacat caaaaaatca
    48121 agatattaat gggatagtag ttcattaaca taaactcttt attctacaaa ataagataaa
    48181 caagaagcag aacaacttag agttgtatat gaacaacttt tacaagtcgg atgttgactt
    48241 gaaaatagca aacctcataa tttttaacct tcaattcaac ctccaataca aaacctctag
    48301 aatcaataga agcgaaattt ttacttgcat atcataaatc ttaatgtctt tcatttacat
    48361 cttttgctta atctaaaata attaattaat gctgcagctt ctgctgcagc cggagctgca
    48421 gcacttttca ttgaattttt tttatatttt ctaacagcac taaaattaac aatgagtaaa
    48481 aaattaaact cctctaataa aaaattcaaa tgctcttatt gtgaacagac atctaataaa
    48541 aaatcaaaca tcaaaataca tgttcaaagg aaacacaaaa agcgaatggc gtacactaga
    48601 tgcgactcac actgttatca aaaataacaa cataaggcaa aatactgaaa ctatgtcaat
    48661 tgatgtaaaa aattcttttc aacagccatt gcctctattt cattacgatc gatattattt
    48721 taataaagaa gaggaagagg atgaagaaag aaaaagaaaa aatgaaagaa gattccgaag
    48781 aatgctttat agtaactttt atgcaatgca ttatattata aacaatagta attacaataa
    48841 tcaactaaga tcttttccat tatatataac aaacaaccgt agccaactaa caaatcaata
    48901 tttcactatt gatacaaatc ctcagatgtc aaaaccagat aggaagatac cttacgcaat
    48961 taaattttat aaatgtaaag aatgtttaaa tgaaatgctt ttccctatat ataattttaa
    49021 acaaatagcc tcaataaaag aaatgaatta cgagagtatt tgccaaatat tccataaaaa
    49081 atatcttttg gctaaattag gaaaattact taaagattgt ttattttata aaattaatac
    49141 agacccaagt cacgaaaaga caagtctaaa aacaattacc atccctaatt tgtgtattga
    49201 aacacctatt ccattaatta ttaaagaaac actttctatg atatacaatt ttgatgatcc
    49261 cgctcgttgg cttttcgaat tattaactgt tgaagagttc atcgatatag gagagataga
    49321 ttctgatcat tggataatgc gagcgtataa ttcaaagcaa ggaaatatta tagcattaga
    49381 taaaaatgaa ctaaaggaat tttttactat aactaatgca tcttttggat taattaaatt
    49441 taaaagagat caaattttga gatacatgtt tagttggatt ccgttttcta ataatcctaa
    49501 gatataactt ggaatataat gataggagca ttggagttag aacaatcagg ttttttgaat
    49561 aaactcaatt cttttaattt taagattcag gtataaagta ttagttcctg ttaacttaac
    49621 aatagtatta gctgtgaatg ttcaattcta ataaatgttt acattctcat ttagataaat
    49681 atttgataaa gagaaactga tgtagtattt caaggttaga acaaaatgct tgatgattac
    49741 tatccctgta ctaaaaatta attgtgatcg ttaatatata tataattggt taaaattatt
    49801 tgtatatttt tataattcag aaatcagaaa taaaattcta tctatcaaag atgtaggcga
    49861 aataacttga gttaacaaca tctttagtaa taaccacata acgatatcat atttttctag
    49921 aattcccgat tacaaggaac agcataggtt aaattaatct ttaaaccatt aggatcttaa
    49981 aataattatt gtttttatca tcaaaaagac gaattgtata ggtattggta aaaaaccatc
    50041 tcttataggg tattattaaa cactcctgta gcattattat caaagtatcc ataacaattc
    50101 ataataattc ctaataccat tagaattaat gaattccaga tatacattac cattcttatt
    50161 aaagcgatta acagtcataa taattctata gtatttaata gtccacaatt gggttttgta
    50221 tctattactt ggttgaataa atagagtgaa atgtagaaga tatacaaata ttaaaaaaat
    50281 tattagaaag gacgaaaaaa gttattcata ttctttcact atattgtaac ataaaatatt
    50341 attatagggc ttttttataa taattataac acaatcagct ttagtacttc ataagtttca
    50401 cttttggacg agaatgagat ggtaggataa tagaaataat ataaattaat attctaacaa
    50461 ttgctatctg ataagaatta ataatagttt ataaatatat tcttatatta tatattcacg
    50521 actctatcaa gtgaatatta tccaaatata aatttttaat attaataatc ttgtagataa
    50581 gaataacaat ataaacaata atgagaataa tttaactaat ccataactaa tatcaacgac
    50641 tggtaaagct ggtaaagaat ttgagattta aaatgaatag attgttgaag actcatcagg
    50701 tctaggagta actatctggg ttcaaataac aatctgaaaa attgattcta atttagaaat
    50761 taagcagtca tcaaaataaa tatcactata aacagaatag ataaataata aagaataaat
    50821 tggtaacaaa taaaaatgta aaatcatcta ttacaacaga aattacaata tccggtgaag
    50881 ggtaatatcg aagcagaacc aggaaaaaat attcgtacca tactcattga atgataattg
    50941 ttagattatt attaaaatta ttctattatt taaaaatagt ctatatattt aaatatagct
    51001 aaatagttag gagaacctaa tacatattgc attatttaga agatacggtg agaatgaaat
    51061 ataaaataaa ttcaaaaata aataaaatca atttatatta tatcttttct ttactagttt
    51121 tttttttaat tccagttaca ttgctcaata atggatattc acaaagtgat tctgtaaatg
    51181 atgagcgaga ctctcaaata gatattaaaa aaaaatttat tttatatatt aatctagaaa
    51241 gaataaactc tcaattagat cttagtgaag agaatgtaga atataaaaat ctagataaag
    51301 cattttcaca ggcttacatt actcatacga ttctttttcc taacatcaaa cctgatttgc
    51361 aaacaatagc ccctgaatta tcttctacat tagagaaact tttaactgat ttgccaatct
    51421 ttattaaaga tgttaaaaat aaacctgtca aagaaattaa acaaacaata gatgaaataa
    51481 aaaatacaaa ttctttgata agtaataaaa taataggaaa taacactctc aatgataaga
    51541 attttctaat tcaaagctca cttcaattat taaaggatgc ttctaaccta gttagtattt
    51601 ttagttcatc tgacatagta gaacaaaaaa accttgatta tgatagtata aagggagtac
    51661 tagacagatc aaaatatcaa atcaataaaa tttcatcaga tgatttaaga tataatgaaa
    51721 agtatcaaat attgtcgatg tattcaaaaa tagaacaaaa ttgggatcaa aaaaatttta
    51781 acgctttatc ttcatctatt acttctcttc aaagttattt gacaccatta ttaaatgcca
    51841 ataactctaa cacaaatctt cctaatgagt atcagaatta cttttcaaat attactacct
    51901 tattggataa tgtggttgtc gaaatcagag aaaacaatga ttataaacaa gctgataagc
    51961 atgttatgtc tgcatatcta gataactatg aatatcttga agctcccatt gaaaaaaatg
    52021 atccggatct gatggtcgat atagaacttg acatgcgaga gaatctcagg aaacttatta
    52081 aaggtaatga aaaattaaaa gttattgaac catttgttac tcaaattaaa gctaaattaa
    52141 ataacgctaa acagacctta gaaaatgatc tcacaatagt gaatgatagt aatagtaatt
    52201 caaacaaatt aatttctaaa gaacataatc ttgctgatat tgaagaactt agtaaaggtt
    52261 ttggatcttt tcagggagaa caaaaacaaa tgggtgaatc tacagatcct caaaaacaag
    52321 atgttagaaa tgaaatagac caaattagaa taaaccttca acatgtgtta aatttataca
    52381 aagataataa atatgatgaa gcattactag tttcacgttc agcttaccta gatagttatg
    52441 agaatataga actaccactt agaccaatcg atccagattt tactttagat atggaaatta
    52501 aatttgctga actcagaaac atgatagaac aaagatcgaa atatgaaaat attgtaaaaa
    52561 agattggtga aattcaaaag ggattggatg aatctgaaac gttagtttca ggaattggta
    52621 caatagcacc tggtattgcc ttttcatcgt cattttctat tatatttaga gaaggattag
    52681 aatcggcatt aatcatcgga gcaatcatta catatttaga agcttctaga aatgatcgat
    52741 tcaaaaaaca tgtgtattat ggtataattc tcgctgctgg tgcaactgct cttacatgga
    52801 tcattgcaga attctttatc gaactgtctg gtgcaagtag agaacttatc gaagctatag
    52861 ctggaatttc tgctgtagct gtattattct gggttagttt ttgggttctt aataaaatag
    52921 agacaaaaaa atggatagaa tttgtgaagt caaaggtttg gaaagcaact acaacaggca
    52981 gtgtgatggt atttgtcgcg ctgtcatttt ttacagtata tagagaagga ttcgaaactg
    53041 tcttgtttta tcaagcaatg ttgtcttatg ccaaacacat ggaacttttt gttatattgg
    53101 gaatgattat aggcttagca gtaataatag tagtagcatt tctagtcaga aaactgggaa
    53161 agaagctacc attaagagtt ctatttggtc taactatggg cataggagca tatatgtcaa
    53221 taaccttttt gggaaatgct attcgtgagt ttcaagaagt tggatatata acaaccacac
    53281 atttattcgg aataattcca aggctagatg taaatctagc aactatgact ggtatacatc
    53341 ctactctcga aactgttgtt gctcaactaa ttctattatc tgtatatctt gtcggttcag
    53401 cgtatatcct tgttattcag ccacggaaaa agaaacttat cgaatcctct cgcaaatcta
    53461 tgtcggattt taaaaaataa ttgctattat gttggtaagg ctacatttat aagttagtct
    53521 gacctaatta gtttggccta atatgtatag cttaactgaa atcaagaata gttggaatat
    53581 agataaaaat gaaacaattt cttttttaaa taatatcctc gaaaatgata catataaaat
    53641 tgttttatct gttatggaaa attctaaaac agtttatgaa attcacgaag aaacatctct
    53701 tccgttaagt tccatatata aaagaataaa aaaactagaa gatataggta taatatcaat
    53761 agagaaaatt actattaata ataaaggacg caaattaatt ttttataaaa gtaaaataaa
    53821 aaacttaact tttaagctaa atgagaagga tgtaagtcta ttaataacct aaaaaatctt
    53881 tagttacact gtaattatat tgaaatgaaa taaaaattat tggaatggtt tatcaaactc
    53941 ctatatatat taggagtata tagtaagaat acctttgatt ggaaaaagat taatatgaga
    54001 ttattttatc tttttaagat tattacacct tttatgactt ataatccagt aggcaattat
    54061 aaaaaatcat ttagcttata ttattacgat ctagttcgtt cattatttgt tacacttgct
    54121 ttcacaggta tactctcaac tggctggttt ggaattccgt tagctccacg ttattttact
    54181 gatacaccaa taaataactt agatgtaata ttcgcaattt ttagttcttt aggaagtatt
    54241 tggggtctga tggcatatag agaatttaca aaaaccagag ataatgtttt gttagtcaga
    54301 gaactgtgca aaaaagtaga tccacttatt ggtgattatg actattcaac ttttaatcct
    54361 attactaaaa aatatgaacc aggttacgtt ccgttaggac caactgattt ctgggacaag
    54421 gaaactgtgg ttcctgaatg gttagataaa ggaaaatatt ggcatattat gcttagattg
    54481 caaattaaaa acaaagatat aattcttaaa gttgttaaag ataatgattt gccttttttt
    54541 tctggaagaa gaaacgttgc aacaacttca agagaaaact taaaaactgg tagtatgttt
    54601 aaaagaagat atcgctatgt tgtttgtgtt ccacaatggt tggtaatgaa cactgaattt
    54661 agtaattttt ctagaagtaa actaaatgtg aatgattctg attttggttt attaatgaat
    54721 gctattaata aacaacttgg attagcatca aatgacatca cagaacatga aaaacaacga
    54781 attaaatatt catggagatt tagttcctta ccaatttact taataaagag tttaccaatt
    54841 caagttattt acaaagaaat ttttaataaa accgctaaat atatccccgc agaaaaaaaa
    54901 catattattg aagaaactaa tgttaatttg gcttatttaa gtaatgatga attaattgaa
    54961 tcattactag ttgttgcaag gcttaagaat atcagatcga gaagtataga aaaaattaat
    55021 attcttttac aatttctaaa aaatgaatat gaaaaattag ggttaggtga tggttccaag
    55081 agatatcata accttcacca tagtctggag gttgcatatg ttagtttgca gatgttacct
    55141 gaagaattac atggattcta tttttcagga gaagattatg aatatatctt agtagcaagc
    55201 ttattacatg attatgatcc tcatcaatat aattataaaa ccagtatagg attatttcga
    55261 tttgaaggtc ctaaagttga aaatacaatc agagaactct taaaaattag aattatagat
    55321 gcttatttta gtatgaatga agttgagttc aaaaattatt tccgtcagta tcagtatccc
    55381 ctattaccac cagttgatta tgttactact catcctgagt ttgtgaagcc tcaaaaacct
    55441 ctccaaagtt taataattga agcattgatc tggagaacag attatccatt taaaaaaaaa
    55501 ccgcatgctc aaaggaacta ccacgatcta attgataaaa tttctaattt aattggatcc
    55561 tctgaaaaat acatgctatt atctgaagtt ttatctttag cagatttatc tgtaacatat
    55621 atgagctccg atcctattag tgcatggtac agagtaacaa gtctatatga agaattaaac
    55681 cttccacgat ttgaggctat ttctagaact gatcaatttt tctcagaaat catgaaactt
    55741 gagttatttc aagaattatt aaacaatcgt aatttttctg gggttttcaa gcaacgatgg
    55801 aatttggttt atcagtttta tcatgaggga aatccatcaa ctcaaataaa caggacgata
    55861 gaaaatgctc aaagttcata ttcaaaaatt aacttacaaa ttggtatcaa aactggagat
    55921 tttttgtacc ttattgcatt agaacataaa gatgaatatt ttataggcat aagtactgat
    55981 gaagacgctg tactcaatat taaagataaa tttgccaatt taaaacaaca aaatatatcg
    56041 tgtttttggg gtgatgcaaa aaaagtgtta cctaatcttc cgaggatgtc tattgataat
    56101 attttattta taatgaataa ttttcctgat ttcaataatc ataatggaca agacccagaa
    56161 ttattgttat tatttaaaag agcttcgata ccacttaaat tacatggtac gtttcaaatt
    56221 cttactaata taaagaaaaa tagtgatatg gaaaaagaaa taataaaaat tgcatcaaat
    56281 tgtggatttc aactattgaa aagtaacaat gattgtaaaa tatatttccc tcaatcatat
    56341 attccaaaag aattttctca acatgattca gtttcattat taatatttca tccttgtctt
    56401 aattctacta ttgtcggata atatttgttg acccattcat taccagagtg ttgttaaaag
    56461 tatataaaga aatgaaaaac tagactatta aaaagcaatg acaaaaaaaa tccgggttgc
    56521 aatcgctgga gtaggaaact gtgcttcagc tctggttcaa ggaatccatt attacaattc
    56581 agtaaatgag aaggattgta ttggtttgac tgcatatgat ttaaatggtt atactcctgt
    56641 agatattgaa tttgtagcag cttttgatgt ttcagattca aaagttggcc tagacctgtc
    56701 tgaggcaatt ttttccaacc cgaataatgc tataaaaatt acaaatgtta ataatttgga
    56761 tgttaaagta aataaaggaa aagttttaga tggggtaggt agacaccttg cagaaaaaat
    56821 aaacgtatca aatcatccta catccaatat atccgaggta ttgaaagatt cccaaacaga
    56881 tatccttttg aattatttgc ccgttggaag tcgcgcagca acaaaatttt atgcagaaca
    56941 gtgtttggct gcaggcgttt catttatcaa tgcaattcca gtttttattg catctgagcc
    57001 tcaatggcaa cagtcattta aagagaagaa ccttttgtgt gcaggcgacg atgtaatgag
    57061 tcaaatggga gccacagttg tacataaaac attagtaaaa ttatttattg atagaggtgt
    57121 taaagtggat gatacatacc aactaaacat aggtggtgac atggattttt ataatatgtt
    57181 agatgaggag agattagaag ataaaagaat aagtaaaacc agtgcggttg aagcaatgtc
    57241 gccatattct attcctatga ggataggtcc atcagatttt gtagaatcat taaaaaatga
    57301 taaagtgtgt tatattaata tccgtggtaa aaaatttgga aatattccaa tagaactaga
    57361 cctgaaactt cgggtagcgg atgcatataa tagtgcggga attatgatcg atgcaattcg
    57421 tgctaccaaa ataggaatag atagaggctt atcaggagca ttagaaagtg tttctgcata
    57481 ttgttttaaa catcctccaa tccaaatgtc gtatactcaa gcaaaagaaa aatttctgca
    57541 gtttattaat ggagaacaag acacataata attaatctag aagttattta tttagttcat
    57601 ttttttatca tcaaccaaat aaaattacat gacaaactcc aaactcaaga tcttcttcat
    57661 tttaaattca gtagggctag tttcgtcatt gtttgccaaa aggcagtgaa ttatataaaa
    57721 taaaaagtta tctgatatat tgtattacag ataattttgt tattgcaaag tcgtattcat
    57781 ttaaatgtta ggggttattc ctagcatcat tccacaaata gttaaaataa tcttttgcat
    57841 atcctgctag tcctgggtgt tctgcccaga ttgcaatggg ttcagaacta cccttccgat
    57901 ttccttcttc tcctaataag attaaaactt gagtgccatc accgataact cctccaccga
    57961 acatgttatc tttaattctt acatctgcaa ttcttgaaat agtttttata atttctgtat
    58021 tcgttccttc tgaaacaaga actacaattc ttataccttt atcgtgtaaa cttcgtagca
    58081 taggttgtaa tggtttagct atttcctgtg ctatttgtgg taacgctatt aacaattctc
    58141 tttggctatt ctgaatgatc tcacttactt tacttgcaat gttatagaaa cctcttacta
    58201 cccatatttc tggtttttct tttattccgc ttttttcata aataggcatt agttcattcc
    58261 taatgatttc ctcattactt ttaatttcat tttctttttg gagtcttatt gcatccaatg
    58321 cagtagaagg agattttgga aaaaatgttt gtggcctcga actatctgat tcaagccaaa
    58381 ctttattttc caaactattc aatacttcat aaatttttga gtatggaaca cctgatgttt
    58441 tacttatatc tgctgctgtc atagagccat gctctaataa tgataaatat actttaattt
    58501 cataacttgt taagccaaga ctttccattg attttctcga cttgtcggta atgttcattg
    58561 aaccatttta attatttaac gtaatatatg tatgttcaat tatactttaa gagtaaatca
    58621 ttttaactat ctattttttt agatcttttt gttattatca aaatgttttc aatatttctt
    58681 catgtctcat tctcttttac taacatcaat tgtagaatgg ttctgttatt tttttagaaa
    58741 aattacaatt ctttagttat gtatcgggga aaaatgtaac gatattcaaa atgaaatttt
    58801 tctgcaatga tatcttcaat cgataatcat ctcttaattt ataattgatt ataatcgatt
    58861 ttgtgaatat atatcttatt ataataagta ttcataaata ttgaattagt tttcatgaaa
    58921 atttaaaata tcaattttta tatcatcttt catatatata ataactagta ttatatattc
    58981 aaaaaacata aaatcttttt aagtttatct tttgaaccag tgagtattaa ttattttcat
    59041 atcaaggaaa gtttaataac ctatttaatt tttttttgtc tatatgccta aattcaaatc
    59101 aactcaagat attattagaa ttattgatac taaagatcaa atacgtaatt ttggtgttat
    59161 tgctcatgta gatcatggta aaactaccat gagtgatagt
//
LOCUS       DASVEV010000026         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1170868, whole genome shotgun sequence.
ACCESSION   DASVEV010000026 DASVEV010000000
VERSION     DASVEV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170868"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VF222_01485"
     CDS             complement(<1..1272)
                     /locus_tag="VF222_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7141129.1"
                     /translation="MENEQINIEELHPIETKIISSLNTFSQIYLTNLVESTNLTLDQL
                     RRGLEWLKFKNIIKVEKTSTLISLDELGRNAIKRGLPERSLVKAIRDGNTTIPAILEK
                     GLLVQNEINIAISKAKSNKWIDLSTSNTDGLTFKLTENFENASGEELLLNKIFSSIQL
                     KESELTDSEIDFLKSLLKRPKFIRRKKEEEKVSLTYLGKKIKTKIDTENRNDERSSKN
                     ENVTSLVPEYLISGKWKNLTFRPIDVDAKVPMANPGRKHPLTDLIDEIREAFTSLGFV
                     EIDGDYIQSAFWNFDALFTPQDHAARDMQDTFYLSHKIKPKLGNKELVKKIADAHIQG
                     WKDSWNINKAKKLVLRTHTTPVTLKYLSETKPEEARIFTIGRVFRNEKVSYKHLVEFN
                     QIEGVVTGKRITLRDLMGIQTEFYKKIGITKI"
     gene            1334..2515
                     /locus_tag="VF222_01490"
     CDS             1334..2515
                     /locus_tag="VF222_01490"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7141130.1"
                     /translation="MESDPNINTNNINNSFEHTKDNNDIIVTPWEVLGDLEDKTYIKL
                     VEKFGTQSISLELLEKIKKLTGEIHPLIKSQYFFSHRDLDWLLTKYENGEHFYLYTGR
                     GPSGFIHLGHILPWLFTKYLQDKFGSKLLFQITDDEKFLYNLGATKETISKYTSENIL
                     DIIAIGFRPNLTRIIIDTKHIHHLYPIATEIARKITFSTAKAVFGFTNTTNIGMIGFP
                     PIQAAPCFLPSIIEKKPTPVLIPAAIDQDPYWRITRDIAAKLGFYKPAQIHSKFLPSL
                     SRVSKMSSSRPETAIFTTDEPEIVEKKVLSSFTGGQATVSLQKKLGGNADICPVYSYL
                     KYFFDNEKESLERYIRCKSGNLLCGECKSDLANNTRDFIIEFKRKREKAKDKVKDFMF
                     E"
     gene            2587..2850
                     /locus_tag="VF222_01495"
     CDS             2587..2850
                     /locus_tag="VF222_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141131.1"
                     /translation="MFSIQKDESIFVMDVENLIDNEIIIKLKDKSCHSIMLKNKDNAN
                     SLFLLIKDIKNGKRRFVSLEKLNSNEEIQIITETIFFNNIDKN"
     gene            complement(3046..3522)
                     /locus_tag="VF222_01500"
     CDS             complement(3046..3522)
                     /locus_tag="VF222_01500"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF001985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7141132.1"
                     /translation="MEKLNLVATGGTFDYIHIGHIKLLSTAFDTSHHVIIGLTSDDFV
                     KRFKSTSNIKNDYLRRLFNLKENISLYFKNVKYTIIKLEDELGPLIYSSKIQGIIVSE
                     ETLVKVKQINEIRISNHLDPLRIIVVEIAKAADGQPISSTRIRNGEIDEKGKILRK"
BASE COUNT         1235 a          597 c          538 g         1346 t
ORIGIN      
        1 aatttttgta attccaatct tcttatagaa ttctgtttgt atacccatta agtctcttaa
       61 tgttattcgc tttcctgtta ctactccttc aatttggtta aactctacaa gatgtttata
      121 agatactttc tcatttctga aaacccgtcc aatagtgaag attcttgcct cctctggttt
      181 agtttcagat aaatacttta aagtcactgg cgtagtatgt gtccttaaaa ctagtttttt
      241 agctttgttg atattccagg aatccttcca tccctgtata tgtgcatcag ctattttttt
      301 tacaagttct ttatttccaa gcttgggttt gattttatgt gataaataaa aggtatcttg
      361 catatccctt gcagcatgat cttgaggggt aaagagggca tcgaaattcc aaaacgctga
      421 ttgtatataa tcaccatcaa tttccacaaa cccaagactt gtgaaggctt cgcgaatctc
      481 atcaatcaaa tcggtcaatg gatgttttct tcccggattt gccattggca ctttagcgtc
      541 gacgtcaatt ggtcgaaatg tcaaattttt ccattttccg gatattaaat attctggaac
      601 taatgaagta acattctcat ttttggaaga tctttcatca tttctatttt cggtatctat
      661 tttagtctta attttctttc ctaagtaagt aagagatact ttttcttctt ctttttttct
      721 cctaataaat ttaggtcgtt ttagcagtga cttaaggaaa tctatttcag aatctgttaa
      781 ctcagactct tttaactgta tagaagaaaa aattttgttc aatagcaact cttccccact
      841 tgcattttca aaattttctg ttagtttaaa agtcaaacca tctgtatttg aagtggataa
      901 atctatccat ttattagatt tagccttgga tattgctata ttaatttcat tttgcaccaa
      961 caaccctttt tctaatattg ctggaatagt tgtattgcca tctcttatag ctttgactaa
     1021 acttctttct ggaagtccac gttttatggc atttctccct aattcatcta atgatattaa
     1081 agtagaagtt ttctctactt taattatatt tttaaacttt aaccattcta ggccccttct
     1141 gagttggtca agagtaagat tggttgattc caccaaattg gttaaatata tttgagagaa
     1201 agtatttaac gaactaatta tttttgtttc gattggatgc aattcttcga tattgatttg
     1261 ttcgttttcc aagtaattct aatgacaatt atttttaaac gtttaaacgt ttgtaatttc
     1321 actatttaga aaaatggaaa gtgatcctaa tattaataca aataacatca ataattcttt
     1381 tgaacatact aaagataata atgacattat tgttacacca tgggaagtcc ttggtgattt
     1441 agaggataaa acgtatatta aattagtaga gaagtttgga acacaatcaa tctcactaga
     1501 attattagaa aaaataaaga aattaacagg ggaaatccat ccactgatca aatcacaata
     1561 tttcttttct cacagagatc tggattggtt actaactaaa tacgagaacg gtgagcattt
     1621 ttacctttat actggaagag gaccatcggg ttttatccat cttggccata tcttgccctg
     1681 gttatttact aaatacctac aggataaatt cggttctaaa ttattatttc aaataaccga
     1741 tgatgagaaa tttctctata atcttggagc aactaaagaa actatttcta agtatacttc
     1801 agaaaatata cttgatataa tagcaatagg tttcagacct aatttaacac gaataattat
     1861 agacacgaaa catattcatc atttatatcc tattgcaaca gagattgcaa gaaaaataac
     1921 attttctacc gcaaaagcag tttttggttt tacaaataca acaaacatag gtatgattgg
     1981 ttttcctcct attcaagctg ctccttgttt tcttccttcg atcattgaga aaaagcccac
     2041 ccccgttcta attcctgctg caatcgatca ggatccctac tggagaataa cgagggatat
     2101 tgcagcaaaa ttaggctttt acaaacctgc tcaaatacat agtaaatttt tacccagttt
     2161 gtcaagagtt tcaaaaatgt cttcttctag acccgaaact gcaattttta caacagatga
     2221 accagaaatt gttgaaaaaa aggtattatc ctcctttact ggtgggcagg cgacagtatc
     2281 gttacagaaa aaattaggag gtaatgctga tatatgtcct gtatacagtt acctaaagta
     2341 tttttttgat aatgagaaag aatcattgga acgatatatt agatgcaaaa gtggaaattt
     2401 actttgtgga gaatgtaaaa gtgatctagc caataataca agagacttta taattgaatt
     2461 taaacgaaaa agagaaaaag ctaaagacaa agtaaaagat tttatgtttg aatagaaatg
     2521 cctagcatac ggaagttaaa ccatattatt tgtttcgatg aatatgaaaa ggagaaactg
     2581 actggcttgt tttcaattca aaaagatgag tcaatttttg taatggatgt cgaaaattta
     2641 atcgacaacg agataataat taaattaaaa gataagtcat gtcatagtat tatgttaaaa
     2701 aataaagata acgcgaattc tcttttctta ttgatcaaag acataaaaaa tggtaaaaga
     2761 aggtttgtat ctctagagaa actaaattct aatgaggaga tccaaataat aacagaaaca
     2821 atttttttta ataatataga caaaaattag acaaaaatta catccctgtt cgaatcttct
     2881 tgtttttgtt attattaata attttatttt ttttacgctt agtaatattt caatcttatt
     2941 tttatcactt ttagcagaac ccaaataaca gaatattcaa aatacattta ctttaaatct
     3001 ttaattaaaa attacattgt attgttaaaa aaattgtcct cttgatcatt tccttaaaat
     3061 cttccctttt tcatcaattt caccatttct tattctagta gatgatatag gttgtccgtc
     3121 cgcagccttt gctatttcaa ctacaataat acgtaatggg tcaagatggt tactaattct
     3181 gatttcattt atttgcttca ctttcactaa agtttcttca ctaactataa tcccttggat
     3241 ttttgatgaa taaataagtg ggccaagctc atcttctaat ttaattatgg tatatttaac
     3301 atttttaaaa tacagcgaga tattttcttt taaattgaaa agccttctta gatagtcgtt
     3361 ttttatgttg gaagttgact taaaccgttt tacaaaatca tcactagtga gtccaataat
     3421 tacgtggtga cttgtatcaa aagcagtgga aagtaatttt atatgaccaa tatgaatata
     3481 gtcaaaagtt ccacccgttg cgacaagatt taatttttcc aatcgtcttt atcaacataa
     3541 ttcagtaata aaagaaaatc attctattat taggattatt ttacaaagat tttgatggct
     3601 tgtggtggac acacattttc acatgctaga caaaatatac aatctttttc tctagccata
     3661 aaaggttttt tctcagaacc tggatgacca ggtgtttcca accattcata cacatt
//
LOCUS       DASVEV010000027         9854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_5699, whole genome shotgun sequence.
ACCESSION   DASVEV010000027 DASVEV010000000
VERSION     DASVEV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5699"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF222_01505"
     CDS             complement(<1..224)
                     /locus_tag="VF222_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141133.1"
                     /translation="MSALNEIRKFFQGLQTLYQTYLPKVDPILVNDLLSRQQKDPDSA
                     PFYMVEMFTEDGTDEEAKREFIIRKTGMAPA"
     gene            complement(1075..1278)
                     /locus_tag="VF222_01510"
     CDS             complement(1075..1278)
                     /locus_tag="VF222_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141134.1"
                     /translation="MRVLTIDKKIFSNDFKERNLINNTFCSKWCSGFRSRSPRHYYGK
                     EERPNTGLAEISLLPAEGKCFST"
     gene            1957..2427
                     /locus_tag="VF222_01515"
     CDS             1957..2427
                     /locus_tag="VF222_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141135.1"
                     /translation="MNDCRHESKKLVCQKQSNNWNTIWKNVKNTKKILEQIITSIGGE
                     PTQEKLGLSLPSYPVDMKAMMDKTMTKQEYELKRAEEDMILENAEVCSYLMMIQKCQM
                     AGGVYLNAIDPLSKNMQDEQNQADWIKTNSPNMLAQLWPKIQSAVAASAPPFSL"
     gene            2457..3686
                     /locus_tag="VF222_01520"
     CDS             2457..3686
                     /locus_tag="VF222_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7141136.1"
                     /translation="MKALVYHGPWDVQIDDKPLPTIQHPEDAILRITSTAICGSDLHL
                     YHGTVPGMQPGQTLGHEFMGVIEEIGPEVHEVQVGDKVVVPFNVNCGQCWFCKNNMWS
                     QCDRANPKTKDIGAAYGYTQLLGGYDGGQAEYVRVPFANAGASLKVPEGIKTDDQVLF
                     LSDILPTGYFAADIANVQPGDDVVVFGAGPVGYFAVMSSFLRGAARVCSVDHWPLRLN
                     KTKDLGAELINFDNEDPVDVIKKETNGKGAICIDAVGFEAVGHMAGNVSDGNGGLLNS
                     SSIKGNNNDNNSNKKTNHDHSKISNPEYNPVNPLQVITWMAQVAKKYSTISIPGVYGS
                     GYDQFPLGLLFNRNIQIHMGQCPVKKYNERLLHLIETGRIDATKVISHTMKLNDAPKG
                     YGGFDKKADIMKIVLKP"
     gene            complement(3883..4092)
                     /locus_tag="VF222_01525"
     CDS             complement(3883..4092)
                     /locus_tag="VF222_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141137.1"
                     /translation="MFHNYDDNKFKKIIDDFILSSTNDTSLSSAIRNIDFEAAKLGIS
                     FYQMIFILIQKDKSNRKKKPLDKYL"
     gene            complement(4655..4822)
                     /locus_tag="VF222_01530"
     CDS             complement(4655..4822)
                     /locus_tag="VF222_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141138.1"
                     /translation="MSTTEHRNNDLDPKNQEVEKTKQEEAKKKKLLIEGKEPQNPLPV
                     GEGHETEVINE"
     gene            complement(4890..5102)
                     /locus_tag="VF222_01535"
     CDS             complement(4890..5102)
                     /locus_tag="VF222_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141139.1"
                     /translation="MTCKGICQRHKASRPKTGKRYLLGQKRCQGCEIFLDWQGIWCPC
                     CGYRLRVNPRHSKFKLMLRTIKNKNN"
     gene            5623..7236
                     /locus_tag="VF222_01540"
     CDS             5623..7236
                     /locus_tag="VF222_01540"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141140.1"
                     /translation="MSSNVPLIANDKVNHVFKYILLSSIPLLIISLISVNPSLWVTSP
                     LHHFYIELFDSMLAGIVAFYYIYRAQTLNDKFSLFLGIGFLVNTLIDLFHVLVSLLDM
                     DNMLFLKYFIPQTWFVGRLFLSAMLAIAIVKYTSFLSINPSTQQKQLSSDMNYQNYNL
                     SRLLLFYLIIITLFVFIVAISSLFVVFPFSVIDDIPIHRPYEIFPLALFLISLYYFYQ
                     NTIYKNNDIFYKSLVISIVFDIFGQIKMSSSAQHFDTAHNIAHILKDASYFINIIGFA
                     LSSIQYNIKLRESNEILTIQYEKVKASEKMKEEFINIAEHELRTPIKPILGLSDIMYP
                     KIKDKEQRELLEIIIRNAKRLKRLTDNLLDITKIEGRSLMLNKEKLNLNTLISEVLKN
                     YVNKQKNQQKVEIVYDFKHKEDITIEADRDRLVQVIHNLLDNALKFSTQNQQMIIVII
                     DKKKERKEKEVIVSVKDTGEGISEKILSKLFTKFATSDPTNGTGLGLLICKNIIEAHG
                     GSISAVNNSNGKGALFRFTLPVKSNKSKQ"
     gene            complement(7833..7907)
                     /locus_tag="VF222_01545"
     tRNA            complement(7833..7907)
                     /locus_tag="VF222_01545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7870..7872),aa:Val,seq:gac)
     gene            complement(8149..8451)
                     /locus_tag="VF222_01550"
     CDS             complement(8149..8451)
                     /locus_tag="VF222_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141141.1"
                     /translation="MLTHNVFPLREWHLEHMQKTVVKFVTGLSDNPSRWEKRQNKKYN
                     NIFCVQRRLNHDIKHGVTNEEIQLFLQKIGNDSSFKDLRNKEGFKERFLEMETFLS"
     gene            complement(9440..9727)
                     /locus_tag="VF222_01555"
     CDS             complement(9440..9727)
                     /locus_tag="VF222_01555"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141142.1"
                     /translation="MKNRSRYEVIAAILSSCIRMETRTKIMYKAMLSNDQCKLYIQFL
                     LKCGLIKEMTNGEKRYYSITESGNKFLFNFEKLKQMLPTIMEEDIHDHLTV"
BASE COUNT         3578 a         1365 c         1429 g         3482 t
ORIGIN      
        1 gctggtgcca ttcctgtttt tctgattata aattctcttt ttgcttcttc atctgttcca
       61 tcctctgtaa acatttcaac catgtaaaag ggtgcactat caggatcttt ctgttgtctt
      121 gataataaat cattaactaa gataggatct acttttggta agtatgtctg atataatgtc
      181 tgcaaccctt gaaaaaactt tctgatctca tttaaagcag acatataaat aatacgagaa
      241 ccagagtata tgagcatttc taaaatctat tttgatatgt tctgtctata ttttaagaca
      301 gattatgaat attaaaagct ccgaaataac cttctatttt aaaaataaaa tctttttaac
      361 attcacttat gttttctgtt aggtttatta atcaatataa aaaataccat aatcattaaa
      421 aaagacggtt aatgtatttt tagtttgatg cgagccttta gtttttatat atattcttga
      481 aatgtctcct cctttattga agtacgattg attaatcaat tttacgaaat agtgaaattg
      541 atactacaaa aaatttgaat ctctaaggag tgatgaatgt taaaagagct gataaaatga
      601 ccaaagagaa aaattattat tacgaggaaa catatagatg aataaaaaaa ggaatgaata
      661 tttcaaatat ctttgataat ctaattaata taatttatgt gtccattttg gtcctaatac
      721 ttaggcctta gattgaaatt tatgatcttt agagataaac tattgggaat taaaattatt
      781 catttccttc aaacattaat ttgtctaagt attataatcc tcgtgtggat cgttctcagt
      841 cttaattaaa aatgcacttt aatgtaattc caatattaaa taaaataatt gtttaattat
      901 atggaaataa tgttgttgga attatagtct atatggaagc gagtgatatg ttttaaaaat
      961 taaatttaac tataaaattt tcatactaat gagaatagat agtaaaaatg tatcacacat
     1021 taaattaaaa tattactcct aaaggttata taatttctgg gtctaaaaat aaagtcatgt
     1081 ggaaaaacat tttccttctg caggtaagag gcttatttcg gccagtcccg tatttggcct
     1141 ttcctcttta ccataatagt gtctcgggga acgggaccga aacccggaac accatttgct
     1201 gcagaaggta ttatttatta aatttctttc tttaaaatcg ttactaaaga tcttcttatc
     1261 tatagttaag actctcacct attcgatgtt tcatagatat cgataaaaga ataaaaacac
     1321 ttcatcagaa aaaaatatct ctttacaatt gttaacaagt aataatagat gtagggatag
     1381 aaaaatttat tttaaattat aaaattacaa aaaataaagt atttgctttt ttatttaaat
     1441 atcatttagt aatagctcac aaataatagg tcaaaatctc ggtaaaatta aaagacgttg
     1501 ttcagaattc tgtaaataca ttaaattgaa atagtaataa gattaagaag acatttagct
     1561 atataataaa aataatatac aaatgaatca aaataattac aactatagta aagacagaac
     1621 tttggttttt accgctgtgt ttgagttcta tgatctattt gcctcaagca atggtaattt
     1681 gtagttattg agatttgttt gcgtgtaaat tattggtaat catggagaac aaaacttgag
     1741 taaaattagc ttacttgacg aataataatt aaaataatta ctttttgtat aaaatttcaa
     1801 ttattttata ttttttattg taatagttat aatttctctc taatacttat agaaaatatt
     1861 actaacaatt tattatgtct aaacctataa ttgatataat gaaccaaaaa ttcattttgg
     1921 aattaaatgc tgctcttgct atggaaaata caggtattga acgattgtag acacgaatcg
     1981 aagaagttag tgtgccagaa gcaaagcaac aattggaaca ccatttggaa gaatgtcaag
     2041 aacaccaaaa aaatcctaga gcagattatt actagcatag gtggagaacc aacacaggaa
     2101 aaattaggat tgtcattgcc ttcctatccg gtggatatga aagcgatgat ggataaaacc
     2161 atgactaaac aggaatatga attaaaaaga gctgaagaag atatgatttt agagaatgca
     2221 gaagtatgta gttacctcat gatgatacaa aaatgtcaaa tggcaggagg agtatatcta
     2281 aacgcaatag atccattatc caagaacatg caagatgaac aaaatcaggc tgattggata
     2341 aagactaatt ctcctaatat gcttgcacag ttatggccaa agatacaatc agctgttgct
     2401 gctagtgctc ctccattctc cttataaaaa taaaagactg agaggtgatt attagaatga
     2461 aagcattagt atatcatgga ccatgggatg tccaaataga cgataaacct ttacctacta
     2521 tccaacatcc cgaagatgca atactgcgta tcacatcaac tgctatttgt ggttctgacc
     2581 ttcatcttta tcatggtact gtccctggga tgcaaccagg tcaaacatta ggacacgaat
     2641 ttatgggagt aatagaagag ataggaccag aagtacatga agtacaggta ggggataaag
     2701 tagtggtgcc gtttaatgtc aattgtggtc aatgttggtt ttgtaaaaat aatatgtggt
     2761 cccagtgtga tagggctaat cccaaaacaa aagatatagg agcagcttat ggttatactc
     2821 aactccttgg tggatatgat ggtggacaag cagaatatgt ccgcgtacca tttgcaaatg
     2881 caggggcttc gctcaaggtt cccgaaggta ttaagaccga tgatcaagta ttatttctta
     2941 gtgatatact cccaactggt tactttgctg ctgatattgc taatgtccaa ccaggcgatg
     3001 atgtagtagt atttggcgca ggtccggtag gctattttgc agttatgagt tcctttttaa
     3061 ggggagcagc aagagtatgc tcagttgatc attggcctct acgtttaaat aagacaaaag
     3121 atttgggagc tgaacttatc aactttgata atgaagatcc agttgatgtt attaaaaaag
     3181 aaactaatgg aaaaggagca atatgtatag atgctgttgg atttgaggct gtaggacata
     3241 tggcaggaaa tgttagtgat ggtaacggtg gactacttaa tagtagcagc attaaaggta
     3301 acaataacga taataatagt aacaaaaaaa caaatcatga tcattcaaaa atatcaaacc
     3361 ctgaatataa tcctgttaat ccattgcaag tcattacctg gatggcacaa gttgcaaaaa
     3421 aatattctac tatatcaata cctggcgtat acggctcagg ctatgaccaa tttcccctag
     3481 gactattgtt taaccgtaat attcaaatcc atatgggaca atgtcctgtc aaaaaatata
     3541 atgagcggtt gcttcatctc attgaaacag gacgaataga tgcaactaag gttataagtc
     3601 atactatgaa gcttaacgat gcacctaagg ggtatggtgg atttgataaa aaagcagata
     3661 taatgaaaat tgtactaaag ccatgaaata acaaataaca gataatgaac tctcatgcaa
     3721 taatagcgtg gcaagagaaa gtatacttga tgaaatagta aaagtataaa cagaaataac
     3781 aattcttatt tttttaatgt cttattttta gaatattctc ttatggttaa tatttataga
     3841 tttaacttgg acaaatacta tttttttaaa ttataggctc tgtcacaaat atttatctag
     3901 gggtttcttt tttctatttg atttatcttt ttgaattaat ataaagatca tctgataaaa
     3961 tgaaatccct aattttgctg cctcaaaatc aatatttctt atggcactag ataatgaggt
     4021 atcgtttgta gaagataaaa taaaatcgtc tattattttt ttaaatttat tgtcgtcata
     4081 attgtgaaac actggtattg aattgatctg tatacttttt aaatgcttta tagaaatgtt
     4141 agtgacatat tatataaaat gcattgacaa tatgataaga acaatattat atattgctgc
     4201 aagagaaaag ggataaaaaa cgttgtaaag caattgatcc catacagtca tcaaagcttt
     4261 attcttattt tcagatatag atcaacgttt tatgtttaag attatttcgt actactggtt
     4321 aataatcaac aaccttatca agttaagtcg agatattcta tagttgcaat atttattgtc
     4381 tcacaaatta taagttagca catatttctg ttatcttcaa aattctgatg ttgaattaaa
     4441 gatattcgac aacttttagt tcgtaaaaat tctaacattt tttaatattt gaagagaata
     4501 ataaaaaatt aaataaaaaa tatttttaat agtagaggac aagttgggta tattttaaaa
     4561 aatggaaaaa tatacataat tatccataca tataaatttt caaaatacta acgaaaattt
     4621 ctgatctacg gtaagttata ttactgtatt ttttttattc atttataact tctgtttcat
     4681 gaccttctcc taccggtaat ggattttgag gttccttccc ttctatcaat aatttcttct
     4741 tttttgcttc ttcttgttta gttttttcaa cctcttggtt ttttggatcc aaatcattat
     4801 tgcgatgctc ggtagtagac attgtattac ttttaattgg tgagagataa aaaggatttg
     4861 cataattgat gaaatatact ataaaaagac taattatttt tatttttaat cgttcttagc
     4921 attagtttaa atttgctgtg tctagggtta actcttaacc tataaccaca acatggacac
     4981 catatacctt gccaatcaag gaaaatttca catccttgac aacgtttttg acctagcaaa
     5041 taacgcttgc cagttttagg tctacttgct ttgtgacgct gacatattcc cttacatgtc
     5101 attttataac caatatatat gaaaccataa tattctattt aatacaaaca tactatttaa
     5161 gatagataga aggtacaatg cttaggtctc atgtaccaag gaatatagtc atatgtttgt
     5221 cgaagaaaag atggtatgta ttaaatttat caaacgaatt ttattatgaa ctatacgcca
     5281 tcattgtatt atgagaaaac ctattgttaa ataattatta caactacggt taataattgt
     5341 taaataataa aaacgccgtt actatctacg tgttgaaatt ttataccata aatatcttct
     5401 gtatatacag tatgtttatc aaagataaca atttgataga tagtttatta tttcatagta
     5461 tgaagctatg aatatgaata ttgctatatt tcaaagtcgt caatctcgat taacatttat
     5521 cgataattat aataattatc aaaacttaac atcacgtgtc tttatttcca aatcttaatt
     5581 tattcagtcc acctgaaaaa ctaaaaaata agcgcatcca atttgtcatc gaatgttcct
     5641 ttaatcgcta atgataaggt aaatcatgta ttcaaatata ttctcttatc ttccattccc
     5701 cttttaataa tatctctgat ttctgttaat ccatcgttat gggtaacttc tcctctacat
     5761 catttttata ttgaattatt tgattcaatg cttgctggaa tagttgcatt ttattatatc
     5821 tatcgtgctc aaacgttaaa tgacaaattt agtctatttc ttggcattgg atttctcgtc
     5881 aatactctaa tcgatttgtt ccatgtttta gtttcccttc ttgatatgga taatatgtta
     5941 ttcctaaaat attttattcc acagacctgg tttgttggaa gattatttct tagcgctatg
     6001 ttagctatag caattgttaa atatacttct tttttgtcta ttaatccaag tacacaacaa
     6061 aaacaattat cttcagacat gaattatcaa aattataact tatctagatt actcttgttt
     6121 tacctaataa taattacttt atttgttttc attgtagcta taagctcact ctttgttgtt
     6181 tttccttttt ctgtaattga tgatattccc atacatcgac catatgaaat atttccattg
     6241 gcattatttt taatttcgtt gtattacttt taccaaaata caatttataa aaataatgat
     6301 attttctata aaagtttggt tatttctatt gtatttgata tttttggaca aataaaaatg
     6361 tcatcttctg cacagcactt tgatactgcc cataatatag cccatatttt gaaggatgct
     6421 agttatttta tcaatattat tggatttgca ttatcaagca tacaatataa tattaaatta
     6481 agagaaagca atgaaatact tactattcaa tatgagaaag tcaaggcgtc agaaaaaatg
     6541 aaggaggaat ttatcaatat tgctgagcat gaattgagaa ctccaataaa acctatactt
     6601 ggattatctg atattatgta tcctaaaatt aaagataaag agcaacgtga gttattagag
     6661 attattatca gaaatgcaaa aaggctaaaa cgtcttactg ataatttgtt ggatattaca
     6721 aaaatagaag gtcgatcttt aatgcttaat aaagaaaaat tgaatctaaa tactttgata
     6781 tctgaagttt taaaaaatta tgtgaataaa caaaaaaacc aacaaaaagt agaaatagtt
     6841 tatgatttca aacataaaga agatattact attgaagcag atagagatag attagttcag
     6901 gttattcaca atcttttaga taatgcttta aaatttagta ctcaaaacca acaaatgata
     6961 attgttatta tagataaaaa gaaagaacgt aaagaaaaag aagttattgt aagtgtaaag
     7021 gatactggag aaggtatatc agagaagata ttatctaaat tatttacaaa atttgctact
     7081 tctgatccaa caaatgggac aggcttgggg ctattaattt gtaagaatat tatagaggca
     7141 catggcggta gtatatcggc agtaaataat tcaaatggaa aaggagcgct atttagattt
     7201 acattgccag ttaagtcaaa taaatctaaa caataacaac aactcaagac tcgactttta
     7261 ttataataca attagtaaat cattcattat ctataatgat agaaataatg ttacgacagt
     7321 tacgacagtc agtgttaaca agatttccat tgacttcata aaacagattc aatgacgtct
     7381 ttatttctta gtgtaacttt tcactgctag tttcattacc gttattgata ttttactcat
     7441 ttcattttta tagctattta tgaaccatcg ctattaattt aataaaagat aacttagaat
     7501 tttatttcat aagtagttga atatgtgtgc ttctttcaca aactgaaaaa gaatggctcc
     7561 ttggaaatac gaacctgtct aaaatgctat gaacataaga ttaaatcaga taaaaagaaa
     7621 aaaattgaaa attttctaca gttggaagta ttaacactga tagaaaacgg attcttactt
     7681 ctcagattgt gaaagaatat tgtaacaatc ttactatatt tagtaacggt cataatagtt
     7741 ttcgttaagc cagctcctat tataattgat aattttccag tagaatctac taaaattttt
     7801 cactatgatg atcaaaaatg tggaaatttt aatgggccga acgcgattcg aacgcgtgat
     7861 ccccgccatg tcaaggcggt atcctaacca ggctagacca ccggccctcc catcattatc
     7921 tctcttgtta tttttataag tgttatttta tcattaacga tccatctcga aaaaccgtga
     7981 tttaattatt ttttggataa taatatatta tatgtgcaaa aatctcaaag gtattaacag
     8041 tttatccaat gaaaaaaata ggtattaatt agttctgatt ataaaactag cctttaatta
     8101 tcttgatgta tttcagtaaa aaaatgaaaa tctcgttctc tttatatttt aactcaaaaa
     8161 ggtttccatt tctaaaaatc tctctttaaa tccttcttta tttcgtaaat ctttgaaaga
     8221 tgaatcatta cctatttttt gtaaaaataa ttgaatctcc tcattagtta caccatgctt
     8281 aatatcatga tttaatcttc gttgaacaca aaatatattg ttatattttt tattttgtct
     8341 tttttcccat ctggatgggt tgtctgatag tcctgttacg aattttacaa cagttttttg
     8401 catatgttca agatgccatt ctcttaacgg aaaaacatta tgggttaaca caatatatat
     8461 ttcataaact actatttaag ttagcctatt atgatggact ctgaatggta aaatatttcg
     8521 tatttgaagg tctttattca agttttaatt tttaaacagt aaaataaata tttcttctat
     8581 gatgtacttt ttatcatcgt aatttgatat aatttatagc agatgttatt tatattttat
     8641 ttccaagctt tctatgctaa aatttgatat agttaacgat aaaaacgcat aaactaagat
     8701 aattataatg aaaaatataa aaaatagtaa aaaaattgat gttttaaacc aaatatatac
     8761 ctatctagga tgtaaataga aattttttac aatgataagt aaattgacga tatgcgaata
     8821 atttaaagat gaaccattaa atatcaattc cactctataa ttatacatga attagaatca
     8881 tattcctacg gatgaaaaac taatatggta taacataaag gacatataga gttaaagaaa
     8941 ttgaaattag aattacattt attaatttca tattcttcaa ttttcatatc tcattatatt
     9001 attacttaaa ttactcactg ttttaggaaa ataattaaca tgcgaaggaa ataaaattac
     9061 gatatcgcca tataaaaata attgatttga tgctaatcgt atacaatatt ggtattgata
     9121 ccaaagtcaa atcataaggt ttcgtagatt agaaacatag ataaagcagg gatatatatt
     9181 aagtacataa aaaggtcagt gaaaacactc gaaaatcaaa tatatggtga caaattggat
     9241 acatatgtaa tgcactaact gaaggagctc tatttgatat gatagcagga agctaataaa
     9301 aaacaataat tcaatcattc ctgttatgac tttatatagc gaaatacgtc cattcaagag
     9361 aaataattaa taagaaaatc actaaattat atcgcagacc tggtccagat gaaaatgaat
     9421 taaacttttc taagttgatt caaacagtta aatgatcatg tatatcttcc tccattatag
     9481 tcggaagcat ttgttttagt ttctcaaaat tgaacaaaaa tttatttcca ctttcagtta
     9541 tagaataata tctcttttca ccattagtca tttccttaat taggccacat tttaataaga
     9601 attgtatgta caatttacac tgatcattac taagcatagc tttatacatg atttttgtgc
     9661 gtgtttccat tctaatacat gaagatagta tagctgcaat tacctcatat ctactgcgat
     9721 ttttcatctt tatataataa agtggctatt taatataatg agtacgatac tattaaatag
     9781 aagattatct ttcattaaac ataaacaatt tatttccttt tctaatttga attgatttat
     9841 ataaaaaaaa aata
//
LOCUS       DASVEV010000028         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_6384, whole genome shotgun sequence.
ACCESSION   DASVEV010000028 DASVEV010000000
VERSION     DASVEV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6384"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            949..2058
                     /locus_tag="VF222_01560"
     CDS             949..2058
                     /locus_tag="VF222_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141143.1"
                     /translation="MINWVFVEMQVNNTRGTTLLSCILIVIIITISYYDPNKQLSTLF
                     IYAEKDKKGGDDDDQKQIEEEKETSFFADRKNSLQYTYINIKIPQIYQKAANGSTYKF
                     NSTNPNAKTQLDEEKDTNIFTKKNNDGSWRIDGGKPRIDIFTKDAGVLSNKQILQENL
                     SNSIVQSWNYSELKEIGYWYKPTDWRNVEISLIFKLIDSSRSKGNQHDLSLVTRSISH
                     SVMYGETSKEDPPFYCGGSSYHNNLSNDGNVRMKKEEFHVDYERERYNPNIQLGEIYN
                     KIIGFKGIVYNINDTSVKMETWVDLNNAGKGPYKKIHEKIDNGDWGDNMKVCGARTNG
                     QAITWGSPLIIIKSNDFKFDIYDIQINEIIPPSSK"
     gene            2534..>2766
                     /locus_tag="VF222_01565"
     CDS             2534..>2766
                     /locus_tag="VF222_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141144.1"
                     /translation="MNSKMLLLLVLIIISIFPSVITPSSAQEEKLQKQQQKGSGSVNN
                     DSPAGGLTKPIVHVNIEGTENNDKIKGGSGDDIV"
BASE COUNT         1130 a          355 c          410 g          871 t
ORIGIN      
        1 cattgaacat aaacccccaa gtggagttgg ttaataataa taataatgat aatatagtat
       61 catttcttga ccaggtataa atcattgtta taaacaaatg cttaaagtga agagaagaag
      121 aagaagaaaa aagacattga atttttaaaa tacatacata tttagtcttc aaataatcag
      181 aaaattaaat gcttattcta tcaaattacc atagattaaa gttggaacac agataatgca
      241 gtgaatctaa caaaccaggt tcatggaggt tgatgtataa tatgttctct aggctgattt
      301 ttcaggtaaa caacttgaca aattttttaa aaaaaataaa tgagtattga ataaaaaaaa
      361 tatccataat aacatattat aatgatttac gtacctaaaa ttataaagca caccttataa
      421 ttaataaatt caatccatga tctataatac caaataaaga atcctaaagc atatgtaaat
      481 taaatataga gtatttttgt acataaaata ccttatttga taatgcatct ctagtgtcta
      541 atcatctatt atttagttta aaatggtcta atatctttcc atcgttagtc taaaatactc
      601 caaccaaggt tgttccatta ttctcaattt ttacatttag aaatacctat ctgtatttga
      661 ccacaagata accataatta agtataaaaa ataataatga cgatagcaag aagagtctag
      721 gcgaatgctt tttcaatgaa gtatatgatt ctagatttgt aaccaaatgt ataaataatt
      781 tttttcctgg gatcataaga acaatatgat ttcaaaaaaa atttgagtta accattccat
      841 ttttggtata ataacatacc attagaacta atctggcttg tgactcttgt tatattacta
      901 aacttcagac ttgtattata atctttataa atagttatta tatatatagt gataaattgg
      961 gtatttgtag aaatgcaggt aaataacact agaggtacta cattactttc ttgtatctta
     1021 attgtaatta taataactat aagttactat gacccgaata aacaattatc tacattattt
     1081 atctacgccg aaaaagataa aaaaggggga gacgacgacg atcagaagca aatagaagaa
     1141 gaaaaagaaa caagtttttt tgcagataga aaaaattctt tacagtatac ttatataaat
     1201 ataaaaatac ctcaaatata tcaaaaggct gcaaatggat caacctataa atttaatagt
     1261 acaaatccaa acgctaaaac acagttagat gaggaaaaag atacgaatat ctttacaaag
     1321 aaaaataacg atggaagctg gaggattgat ggaggaaaac ccagaataga tatctttacg
     1381 aaagatgcag gcgtattatc caataagcaa atattgcagg aaaacctctc caacagtatt
     1441 gtccaaagtt ggaattattc agaattaaaa gagataggtt attggtataa accaactgat
     1501 tggagaaatg tggaaatttc attaattttt aaattgattg attcatcacg ttctaaaggc
     1561 aatcagcatg atctctcttt agttaccaga tctatatcac atagtgtgat gtatggggaa
     1621 acatcaaaag aagacccacc tttttattgt ggtggttcaa gttaccacaa caatctctct
     1681 aacgatggaa atgtaagaat gaaaaaagaa gaatttcatg ttgactatga aagagaaaga
     1741 tataatccaa atatacagtt aggagagata tacaataaga taattggttt caaaggaata
     1801 gtatacaata ttaacgatac atccgttaaa atggaaacct gggtagattt gaataatgcg
     1861 ggcaagggac catataaaaa aatacatgag aaaatagata atggggattg gggtgataat
     1921 atgaaagtat gcggtgctag aacaaatggt caagcaatta catggggctc ccctctaatt
     1981 ataatcaaat ctaatgattt taaatttgat atatatgata tacaaataaa tgaaataatc
     2041 cctccatcat ccaagtaaaa attataaatt tattttaggt tagtataata aacttgagtt
     2101 taccaaatac tacgattcat attctattaa tggaattgaa aatttagatt ggataatcca
     2161 aatactattg agtatatcat ttcttcaagg aaaaatatag atgaaataga atttaaaaca
     2221 atacaatata ttgctagtaa aaaatctttt tttaataacc tttgcaataa aatatttttt
     2281 acttaatgtc taaaattatt ttcataaaat tgttatgaat gtttctgagc atttaacaca
     2341 atctttacta tccattatat cttaacaagt aaattattag cattattctt aatcatagta
     2401 ctagtagtaa agtagctaac aaagtaacaa tatgttctac aatagtgtta ttatagaact
     2461 tgcagagaaa aaatgattaa aaatactttt taattcagct tgttatttgt tactgaatag
     2521 aggaatagaa gatatgaatt caaaaatgtt attacttttg gtgctaatta taatatccat
     2581 ttttcctagt gtaattacac cctcgagtgc tcaagaagaa aaactgcaaa agcaacagca
     2641 aaaaggatca ggcagcgtta ataatgattc accagctggt ggactaacaa aacctatagt
     2701 tcacgtaaat atagagggaa ctgaaaacaa cgataaaatt aaagggggaa gtggtgacga
     2761 tatagt
//
LOCUS       DASVEV010000029         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1171625, whole genome shotgun sequence.
ACCESSION   DASVEV010000029 DASVEV010000000
VERSION     DASVEV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171625"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="radA"
                     /locus_tag="VF222_01570"
     CDS             complement(<1..1035)
                     /gene="radA"
                     /locus_tag="VF222_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HEX7141145.1"
                     /translation="MESESKSNANVAIIDIDIQDIEGIGPTTAKKLKDAGIISVMDLA
                     VASSEELSESINATKETAASFILSAQKLLRETNVLDKEFVTAEDALQKRRSMLRCSTG
                     SYALDDLLLGGIETQAMTEFYGEFGSGKSQVCHTLCVTAKQPVEAGGLNSSVIYIDTE
                     GTFRPERVEQIARSRGFEPTEILKNIAVCKVYNSSHLELIVKDLGKYIDDFKAKLVVI
                     DSIISLHRAEFSGRGTLADRQQRLNGMLHKLIRLAEIYNIAIVITNQVQSSPDTFFGD
                     PTKATGGNVLGHASTYRIYLRKSGENRIAKMMDSPYHPYSDTRFTVTEKGADDIEEQT
                     PKKGSKRSTAA"
     gene            complement(1174..1309)
                     /locus_tag="VF222_01575"
     tRNA            complement(join(1174..1188,1219..1239,1272..1309))
                     /locus_tag="VF222_01575"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1273..1275),aa:Trp,seq:cca)
     gene            1502..1747
                     /locus_tag="VF222_01580"
     CDS             1502..1747
                     /locus_tag="VF222_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7141146.1"
                     /translation="MIGETQLRARRTRIKILRAIAERNGSAGFSDIQNATALSTGSIY
                     YHIERMRAYIRKNAKNYSITKKGIQLLVEIDSKSKES"
     gene            1828..2103
                     /locus_tag="VF222_01585"
     CDS             1828..2103
                     /locus_tag="VF222_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141147.1"
                     /translation="MKYRSRTDIVALILEATKEGAAKTKIMYKAFLSYSQLKDYLSIL
                     LERELIEYSDKDQIFRITDKGRRYLVLYSKVMELITPSDRDLENKIK"
     gene            complement(2283..2924)
                     /locus_tag="VF222_01590"
     CDS             complement(2283..2924)
                     /locus_tag="VF222_01590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX7141148.1"
                     /translation="MKKFIKYLSFLLAFFLIISLIQFNFDYNYSTLQKTDYLKKQIEI
                     CCTWGNQLADGILTYEINNAKPETKELVISAISYWKENIPGIEFRETDSKQQADIKIS
                     FRNDNGKVAGQTMTNFDLDGFIFNVKISLAEKAFGKQLNKNVIEYIAKHEFGHALGLG
                     HANFNESLMSSLVYHSFNKISDCEREAVMEANKWKLIDNDSSPQINKIKKYFC"
     gene            3054..3398
                     /locus_tag="VF222_01595"
     CDS             3054..3398
                     /locus_tag="VF222_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141149.1"
                     /translation="MSSAEVYSTIYLKYKNLEDMLKVILFASQSPIGMVPMLYHLNFK
                     DKELLFIETGAINTVIHYMILTEKPAKKFIELKQLTGKFEFVNGIGNDTQAIYIPILE
                     LEQSSFNFPVNI"
     gene            3733..>4008
                     /locus_tag="VF222_01600"
     CDS             3733..>4008
                     /locus_tag="VF222_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141150.1"
                     /translation="MILDILTESAKNVYSEIKDLLGTSEGASKISLGAGGDISRKIDI
                     VAETAVLNTIKSNNISPVIIGEECGIVNLNNSSYSSSSSNNNNKGFVI"
BASE COUNT         1382 a          573 c          552 g         1501 t
ORIGIN      
        1 tgctgctgtt gatcttttac ttcccttctt tggtgtttgc tcttctatat cgtctgctcc
       61 tttttcagta actgtaaacc ttgtatctga ataaggatga tagggtgaat ccatcatttt
      121 tgctattcta ttttctccag attttctaag atagattcta tatgtagaag catgacctaa
      181 aacattacct cctgtagctt ttgtgggatc gccaaagaac gtatcagggg atgactgcac
      241 ttgatttgtt atgactattg ctatgttgta tatctctgct aatcttatta atttatgtaa
      301 catgccgttt aatcgttgtt gtctatccgc taaagttcct cttccagaaa attcagctct
      361 atgaagagat atgatgctat caattactac taattttgct ttaaaatcgt caatatattt
      421 tcctaaatct ttaactatta attcaaggtg agagctgtta taaactttac aaactgctat
      481 atttttaaga atttctgtag gctcaaatcc tcttgatctt gctatttgtt caactcgttc
      541 tggtctaaat gtaccttctg tatcgatata tataacagag ctatttaatc cacctgcctc
      601 aactggttgt tttgctgtta cacataaagt atgacaaact tgagatttac ctgatccgaa
      661 ttcaccatag aactctgtca tagcctgtgt ttctatgcct ccaagtaaaa gatcatctaa
      721 tgcataagaa cccgtggaac atctaagcat tgatcttctt ttttgtaacg catcctctgc
      781 agtaacaaat tctttatcta agacatttgt ttctcttaat agtttttgag ctgaaagtat
      841 aaatgaagcc gctgtttctt ttgtagcatt aatactttca gatagttctt ctgaactggc
      901 tacagctaaa tccattacag aaataatacc agcatctttc aatttttttg cagtagtagg
      961 acctatacct tcaatatctt gaatatctat atcaattatt gcaacattag catttgattt
     1021 tgattcactt tccatatcta tgcaaatgat tttctcatat taatatatgc agtaacttta
     1081 tagagggtga gggaggtagt acatattttt tatatatcta ttaatttaaa actctatttt
     1141 tcataattta ttttatgtaa ttatatatat agatggggcc gaagggagtt gttctgatag
     1201 tgatttaatc attatcaatt gaacccttga cctacgggta tcttcaagat ataccagccc
     1261 ttttttttga tctggagccc gttgcgatgc caggcttcgc tacgacccca tgtgacatcg
     1321 cagttatggt tattgatcca tatctctata agctttacga agatataaaa aaatatatct
     1381 agtaagaata aatatgaaat tatcaaattt tagtataaca ttttttatat cttttacttt
     1441 ttattataaa tctttattta attcagtaaa aaataaataa tttatttcag caatatcaat
     1501 attgatagga gaaactcaac tccgcgctcg aagaaccaga attaaaatat tacgagctat
     1561 agctgaaaga aatggatctg ctggtttttc tgacatacaa aacgctacag cgttatctac
     1621 gggatccata tactatcata ttgaaagaat gagagcctat ataagaaaaa atgcaaagaa
     1681 ttattctatc acaaaaaaag gtatccaatt attagtagaa attgattcaa aaagtaagga
     1741 atcataatta gttttcaatt aaatatttat aattttaaat acattacgat caatttttat
     1801 gaaacattcc atttataaaa tctgttaatg aaatatcgaa gcaggactga tatagtagca
     1861 ttaatactgg aagctactaa agagggagca gctaaaacaa aaataatgta taaggctttt
     1921 ctttcttata gtcagttaaa agattatctt tcaatattat tagaacgtga attaatagaa
     1981 tattctgata aagatcaaat ttttaggata acagacaaag gaagaagata tttggttctt
     2041 tattctaaag ttatggaact aattactcca agtgatcgag atttggaaaa taaaattaaa
     2101 taaaataaac ttattttata atataagaaa ataacaattc tgtctattat tcttatatat
     2161 gtttacatta tatagttagg tcatataata gtaaattatc atagatagta tatgacaaca
     2221 atttagaaat aaaaacatgg ttaaaataat taaaatgtga agtaaataat ataaacaatg
     2281 aattagcaaa aatatttttt aattttgttt atttgcggcg atgagtcatt gtctattaat
     2341 ttccatttgt ttgcttccat aacagcttct ctttcacagt ctgaaatctt gttaaatgaa
     2401 tggtaaacca aagatgacat aagcgattca ttaaaatttg catgtcctag accaagagca
     2461 tgaccaaact catgttttgc tatatattca atcacatttt tgttcaattg tttcccaaaa
     2521 gccttttcag caagcgatat ctttacatta aaaataaatc catctaaatc aaaattagtc
     2581 atggtctgtc ctgcaacttt tccattatca ttcctaaaag aaatttttat atctgcttgt
     2641 tgtttactat ctgtctctct aaattcaatt cctggtatgt tttctttcca ataacttatt
     2701 gcagatatta ctaattcctt tgtttcaggt tttgcattat ttatttcata tgtaagtatt
     2761 ccatctgcaa gttgatttcc ccatgtacag caaatttcta tttgtttttt tagataatca
     2821 gttttttgga gagtagaata attataatca aaattaaatt gaattaaact aataattaag
     2881 aaaaatgcaa gtaaaaaaga taaatacttt ataaattttt tcaataaatg aactataaat
     2941 taactcatat attttttctt tgttactaat attatttctc tttgtatact tattttaata
     3001 gataaagacg gaagagtatt ataggttact attataatag ggtatgatat ttgatgtcat
     3061 cagctgaagt ttattcaact atatatctaa aatataaaaa tctggaagac atgcttaagg
     3121 tgattttatt tgcttctcag tcacccatag gaatggtgcc aatgctttat cacctcaatt
     3181 ttaaggataa agaattatta tttatagaaa caggagcaat caatacagtt attcattata
     3241 tgattttaac tgaaaagcct gcaaaaaaat ttattgaatt aaagcagctg actggaaaat
     3301 tcgagtttgt aaatggaata ggtaatgata cacaggctat ttacattcca attttagaat
     3361 tggaacaatc atcatttaat tttccagtta atatataata atttaaattt tcttactttt
     3421 tttggttatt atctagttag cagttgtagt ttcatatttt aaattttcaa tttcttactt
     3481 aacgtttaaa agcaaatgtt gtattataat aacatggaat caaaactttt gtatagtatt
     3541 tatgttggaa taaaagtctc actctataaa ttgataaaag gacgaccaaa aaaaagtttt
     3601 ccaaaagaat cgaacaacaa ttaaatttca tattgtaatt attttttcat atttaattga
     3661 ttgattgatt ttaactttag tatatcatat ttttaactat atcattttat aacattacga
     3721 taacatattt ttatgatatt agatatttta actgaatcag ctaagaatgt ttatagcgaa
     3781 attaaggatt tattaggaac aagtgaaggc gctagtaaaa taagtcttgg cgctggaggc
     3841 gatatttcta gaaagattga tatcgtagca gaaactgcag tgctaaatac aattaaatcc
     3901 aataatatat ctccagtgat aattggagaa gaatgtggaa tagttaatct aaataattct
     3961 tcctactcct cttcttcttc taacaataat aataagggtt ttgtgata
//
LOCUS       DASVEV010000030         8887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_6534, whole genome shotgun sequence.
ACCESSION   DASVEV010000030 DASVEV010000000
VERSION     DASVEV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6534"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..417)
                     /locus_tag="VF222_01605"
     CDS             complement(241..417)
                     /locus_tag="VF222_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX7141151.1"
                     /translation="MTKSFGCSDVGVNCNWTTTADSEEDIMTKIKEHANSVHGFKEIP
                     QDLAEKVKSAIKEQ"
     gene            complement(681..1037)
                     /locus_tag="VF222_01610"
     CDS             complement(681..1037)
                     /locus_tag="VF222_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141152.1"
                     /translation="MESKDILMVNSTIIGGILILLTISSFSENEFPNRSIFVVIAVVV
                     VIIFSISSISIFYDKQEQIIKKYSKMGFIALVGFMIFIGITNIINIISPDIWEKYAIT
                     NKIAFSNTTTFSNSNT"
     gene            complement(1353..1760)
                     /locus_tag="VF222_01615"
     CDS             complement(1353..1760)
                     /locus_tag="VF222_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141153.1"
                     /translation="MSSSENENYEGKDPMSPEKLTSHEPTAVKRDSNDQEIDESEQIG
                     TDSEEAKEKYRRQGMTKVSGDSNLNTNTSYEGNTNTHADNLDSTSYGDVKDDVKEGAE
                     GTFDKLKAGAKAVGKKITDPDRSLEDEYNREKS"
     gene            2457..2999
                     /locus_tag="VF222_01620"
     CDS             2457..2999
                     /locus_tag="VF222_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141154.1"
                     /translation="MQIVSAIDNDKLKFDKVDFKIITLLVLGYDNKKISSTLKIPLST
                     IQRRTRIILLSKIVTQEYIPNFKILGIKKGMLHIYLRDGTLKQRAETISKLDGILSVS
                     IHVGNSDIVAEFVYDNSECLVDVISEIKHMQDIEKVLWSEEIYKVPVSKENILKSFKK
                     YWTDNNKNNNGGQKRDKILY"
     gene            3550..3732
                     /locus_tag="VF222_01625"
     CDS             3550..3732
                     /locus_tag="VF222_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141155.1"
                     /translation="MSNDNRGLASADEKTRKRVASEGGKAPHDERGLQAADEETRKRV
                     AREGGKASSGGRRDKE"
     gene            complement(4025..4369)
                     /locus_tag="VF222_01630"
     CDS             complement(4025..4369)
                     /locus_tag="VF222_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141156.1"
                     /translation="MYSLVDDELDIVDLFKEALEMKGYYVCPFTNPLLALDHIIKDPG
                     RYSLIISDFRMQQMNGNELATKLLQTNTNFKVILMSAYTDVECDPNFQFTSKPITIPQ
                     LLQIVKDSTSSS"
     gene            4618..5493
                     /locus_tag="VF222_01635"
     CDS             4618..5493
                     /locus_tag="VF222_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7141157.1"
                     /translation="MHQILIGCSGWSYADLPEKGGWTKVFYPNGQTKRLSYYSQFFNT
                     VEMDSSFYEKFYKYMTKQTFIGMDKATPDHFEFSIKVPETVTHIKRLDVNKGAISSLD
                     EFLDKISPLRMAKKLGAILIQLPPSFTVTDFKNTENFLERLSSDYHYAIEFRHPSWNT
                     EGPWEMLKHYNIAAVMTDSPILDNLQYLSEITITSKNHSFIRFHGRNPKHRYNYRYTK
                     EELIPWVDKVKEIIAKTNTLRIYFNNHYGGKAVINAMQFKEMLELPLSNKEKIALDHA
                     KNYYSKTTLDTIKDR"
     gene            5557..5715
                     /locus_tag="VF222_01640"
     CDS             5557..5715
                     /locus_tag="VF222_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141158.1"
                     /translation="MKVWVIWNEKETNNKSIDKIFSTEEKALEYIEKVKGGHNSDKYI
                     EEFELDQL"
     gene            6529..6675
                     /locus_tag="VF222_01645"
     CDS             6529..6675
                     /locus_tag="VF222_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141159.1"
                     /translation="MSKDSKNTKGKKKSDTSKRGLASADKETKTKVARKGGKASQSTG
                     KNKK"
     gene            7821..>8887
                     /locus_tag="VF222_01650"
     CDS             7821..>8887
                     /locus_tag="VF222_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEX7141160.1"
                     /translation="MERKEGIIIDEDDFFSRKRKEIGIISVVAIIISSFGLFFYQQNI
                     TEQNIKDSIFNQYKDRQIETTQIISESIGSDLKLIMSILQGIADSVPLQQGELYGDRI
                     HQLMKEKFDQINDITKVDGLFIADEDNIITHNIVSEGKRSFVNIDISFRDYVQETRNT
                     LKPTFSDGFKGIDNIFSIALTVPIVNSDSGKYIGIIGIQIPTEPFFAHSGNINNVDSQ
                     FLVAYDSKKNYLATPRTQFIGMNFFDNDVQNFFNVTDTQNNYYRKVFSGKLLGGSSVY
                     DFGSGERLNTGYPILLQEKPEYFIFVITPTTQIYSHINDILHTERVKGFSLIFGLSFA
                     VLILIIFFIKWNNTLNNEVRK"
BASE COUNT         3374 a         1128 c         1209 g         3176 t
ORIGIN      
        1 ataaaataat gttatataaa tcacgatgtt gtatacaatt ttatttatca ggcaaagatc
       61 tattttcatt ttattaataa agtatctaat attaaggtca atgtagatga attaattcaa
      121 cataaaatat tatatattct gaaaggttaa ataatcatta cattatctat atatttttaa
      181 gataaaattt gtaaaatcat atattaaaaa tggatttagt gtataataaa aaaatagata
      241 ttattgttct ttaatggctg attttacttt ctctgcaaga tcttgtggta tttctttaaa
      301 accatgaaca ctatttgcat gttctttaat ctttgtcatt atgtcctctt ctgaatcagc
      361 agtagtcgtc caattacaat ttacacccac atcagagcat ccaaaacttt ttgtcattat
      421 taaataaaca tgattaatct atttaaggaa ttctatctca aaactttgtc aactttgttg
      481 gttgattcaa tttttaaaag atggtgtcaa aagtcggttg atggatctat aattaatata
      541 ctaacaaact cattatcaaa tcccatatac aatacattat tgaagccata agttttattc
      601 aatcaaaata atagataata acttagaaat tgagttatct ctattatttt ctggaatcgt
      661 tatttttgat ttataggagg ttatgtattg ctattagaaa aagttgttgt attgctaaaa
      721 gcaattttat tagttatagc atatttctcc caaatgtcgg gagatataat attgatgata
      781 tttgttattc ctataaagat cataaatcca accaatgcta taaatcccat cttcgaatat
      841 tttttaataa tttgttcttg tttgtcgtag aaaatactaa tagaagaaat agaaaaaata
      901 attaccacta caactgctat tactacaaaa atagatctat taggaaattc attttccgaa
      961 aatgatgaaa tagtaagtag aattaaaatt cctccaatta ttgttgaatt caccattaaa
     1021 atatctttag actccattaa cacattacca cagttttatg atcaatatac ttattataaa
     1081 tctaaatttc attgtaatgg aagtagttat gaaataacaa aacaagaatg gctgatctga
     1141 aattaatgtg aaagtagagc tgaagtgtca ttcataaaaa tgtacattga ttttgtaact
     1201 taaaatacaa taagtatatg gataagttta ttttaaacat ctataatttt ctgataatta
     1261 tattgaaaat tatttaatac tatataaaac taatgttcga taaaaaatga aaatcaggaa
     1321 aaatagatat ttattttgac ctcttttatc ttctatgatt tctctcgatt atactcgtct
     1381 tctaaacttc tatctgggtc ggttatcttc tttccaactg ctttagcacc tgcctttagt
     1441 ttatcaaaag tgccctcagc accttctttg acatcatctt tgacatcacc ataagaagtt
     1501 gaatcaagat tatcagcatg agtatttgta tttccttcat aacttgtatt tgtatttaga
     1561 tttgaatctc cagatacttt agtcatgcct tgtcttctgt atttttcttt tgcttcttct
     1621 gaatcagttc ctatctgttc tgattcatca atttcttgat catttgaatc tctttttact
     1681 gcagtaggtt catgagaagt aagcttctct ggtgacattg gatcttttcc ttcataattt
     1741 tcgttttctg atgaactcat gatatctagt gaaaaatata atatttaaca attgaatatt
     1801 gtaattttgc tgtttgttgt aattatagat ttagaagttc catctttatg ttagattgta
     1861 aatccaggtg tttggacgcg cttagcatta tcaatattaa ttgataaacc atttaactgc
     1921 ctttttgaat gactaattca acaattatta agctaattta gaatataatt caattataat
     1981 ataaacaata tattattaca gattagagat taaagtcatt attactcact cctactggta
     2041 tccataaagt tattgtttat accaattttg aaatactatg taaaatttac tttactatta
     2101 gaaaagatgg tgtaacgatt cttgcacata ttggaacctt aaaaaaacga aagaatagaa
     2161 aaggtattag aatatgaaaa ttctactctt tcttcctttt acaatataaa aaagcaaatt
     2221 cgtttatcta agcaaattat tatgataatc gtttagtaat agtaaatgtt gtaaagtcgt
     2281 atatgctcaa atgatgttaa aaactatttt tactctctaa tatctcatac aaatcattta
     2341 tttgttcaat tgtgtatcaa gacactattt ttaatagaaa atgttataaa cgatgtttgt
     2401 tactatataa ttagatatat aatatgacaa acttttctag gttctgctgc agttaattgc
     2461 aaattgtaag cgctatagat aatgacaaac ttaagttcga taaggtagac tttaaaataa
     2521 ttactttgct tgttcttggt tatgataata aaaaaatatc gtctacatta aaaatacctc
     2581 ttagtactat tcagagaagg actagaatta tattactttc aaagatagta actcaagaat
     2641 atattccaaa tttcaaaata ttaggaatta aaaagggtat gttacacatt tatctcagag
     2701 atgggacttt aaaacagaga gcagaaacaa tatcaaaact agatggcata ttatcagtat
     2761 ctattcatgt aggaaattct gatatagttg cagaatttgt atatgataat agtgaatgtt
     2821 tggtagatgt tatttcggaa attaaacata tgcaagatat agaaaaagta ctttggtcag
     2881 aggaaatata caaggtacca gtgtcaaagg aaaatatatt aaaatcattt aaaaaatatt
     2941 ggactgataa taataaaaat aataacggtg gacagaaaag agataaaatt ttatattaat
     3001 attaccaaga attacaaatt taagtattac taatctacta tttatctaat taaaaaagta
     3061 attagctttt tccttcccat aatggaattg gtttttaaat gacaatattc tctatttctg
     3121 gtatgtattc cttattttta tacatgtatt tattatttgc agtaataacg ccaattttac
     3181 ttattattta ttcttcaatt tattatacaa taatatgttg tctatcaaac tctgtctaat
     3241 tagatccaaa gattgtctat tataatattt ttcctattgt ttaggcacat ttattataat
     3301 tattagcatc caaacctatg aaaatatata ttatctatat tttctggtaa ttatccagtc
     3361 attatctaat ttttcttttg acttgtttag tcttttattt gtctatataa tggttgtcta
     3421 tataatggta aaataagaaa tataattcca attatcaaag atgttaaaga aatatagtca
     3481 aactattatc tctatacttt tagataaatt atatccaagt tttataaact atgtaactac
     3541 ttttagacaa tgagcaatga taatagagga ttagcctctg cggatgaaaa aacaagaaaa
     3601 agagtagcaa gtgaaggtgg aaaagcccca catgatgaac gtggattaca agcagcagat
     3661 gaagaaacaa gaaaaagagt cgcacgcgaa ggtggaaaag catctagcgg tggccgcaga
     3721 gataaagaat aagaatagca aaaaaagaat aatttcctta agttgaggaa aataattcaa
     3781 tttttatttt aagataagtt tttacttatg aaattttaga ttattgttgg ttaacaattt
     3841 aaatctacat gagaaaataa taaccaatta ctattcaatt ctttaaaatt aaaatttatt
     3901 gtgttaggct caaccctact tccacttatt gcaaaactct taaaagtgtt ttactattaa
     3961 acgatatttt cttattttgt tggtgctcgc ccagtatttt atcttatcaa tcagagtata
     4021 catattatga gctacttgta ctatctttta caatttgcaa aagttgaggt attgttattg
     4081 gcttgctagt aaattgaaaa tttgggtcac attcaacgtc agtataagca ctcattaata
     4141 ttactttaaa gtttgtattg gtttgcagca gtttggtagc taattcgtta ccattcattt
     4201 gctgcattct aaagtcagaa ataattaaag aatatctacc aggatctttt ataatatgat
     4261 ccagtgctaa aagtggatta gtaaaaggac aaacataata gcctttcatt tctaaagctt
     4321 ctttgaataa atctacaata tcaagctcat cgtcaacaag gctatagatt tagttgacat
     4381 ttcaataatg aaatattaat cctttgatat ttaagtataa ttctgtgaaa gaattgtaaa
     4441 attttttgat ttatagtatt atttataaag gatttataac tattaaaatt gtaagagcat
     4501 acagaatgaa aatttataca acatttacta tttttaatgg tgttaaattc atgtattcaa
     4561 atatatattc aatttgaatg attttattta taatgaaatt gtattttaat tgatactatg
     4621 catcaaatac taattggttg ctcaggatgg agttatgcag accttccaga aaaaggaggt
     4681 tggaccaaag tcttttatcc taatggtcaa acaaaacgtc tttcgtatta ttctcaattt
     4741 tttaatacag tagaaatgga ttcttccttc tatgaaaaat tttacaagta tatgactaaa
     4801 cagactttta ttggaatgga taaagctaca cctgatcatt ttgaattttc gataaaagtt
     4861 ccagaaacag ttactcatat aaaaagatta gatgttaata aaggggcaat ttcatcacta
     4921 gatgaatttc ttgataaaat ttcaccactt cgaatggcaa aaaaattagg tgctatactt
     4981 atacaattac ctcctagctt cactgtaacc gattttaaaa atactgagaa ttttcttgag
     5041 agattgtctt cagactacca ttatgcaata gaattcaggc atccatcttg gaatacagaa
     5101 ggtccatggg aaatgttgaa gcattataat attgcagcag taatgacaga ttctccgata
     5161 ttagacaatc ttcagtatct ttctgaaatt acaattacct caaaaaacca ttcatttatc
     5221 aggttccatg gaagaaatcc aaaacacaga tacaattatc gttatactaa agaagaattg
     5281 ataccatggg ttgataaagt aaaagaaata attgccaaaa caaatactct ccggatttac
     5341 ttcaataatc attatggtgg taaagcagtc attaatgcaa tgcaattcaa agagatgcta
     5401 gaattaccac tttcgaataa agaaaagatt gcattagacc atgcaaagaa ttattattca
     5461 aaaactactt tggatactat aaaagatcga taatatttgt aataaattta aaaataacaa
     5521 taatttaaaa tcatgcgatt tattagatat aaaataatga aagtttgggt tatatggaat
     5581 gaaaaagaaa ccaataataa atctatagat aaaatctttt ctacagaaga aaaagcgtta
     5641 gaatatattg aaaaagttaa aggaggtcat aattctgata aatacattga agaatttgaa
     5701 ttagatcaac tttgaaatca aaaatttttc tatcaatcaa atttaaatta tataatatat
     5761 ctattacaat tctttaatta ttaaatttgg ttatgattta aaaaaaacaa actttgttaa
     5821 cattattatt ctttaattta ttcatctata tttacaattt tttacattca gcataatatt
     5881 ggatattact atatatgtta ataccttatc gctaattgtc ataatttctc cagaatgtag
     5941 agaaacggcc ggaagaccat agattcaatt tcatagttaa tggtttcaac aataatggaa
     6001 atattaatgg gaagtttatt aattctgaag gaataaagga ctagtacgat cactttaact
     6061 aatgaaacgg aggttttaat gcctatacaa ttgcagagga atttgaacct aatacgtatc
     6121 tttaagattc tttgatgata aaaataatac ttttgtcaaa gattctaatt catcaaaagt
     6181 tgttttagat tataaagtac attctgaaga acaagaataa aactttgaat gtggttgtta
     6241 actgatttca tgcaataatt cgattaactt attactactt tatcccttat caaatttcta
     6301 tttcaaaggt aaaaatttta tctaaataat attgtatgtt aaattgaaag aaatgagtaa
     6361 aaatacattt atttggattt tgcttttaat tattgtgttt aaagcattag cattttcata
     6421 tataatttca ctattcatca aataatagat aaagaaaaat ctaccgaata taataacata
     6481 ataattatta aaaattatta aataatataa ccaagtagta cgatcaatat gtctaaagac
     6541 agtaaaaaca ctaaaggtaa gaaaaaaagt gatacaagca aaagaggatt agcctctgca
     6601 gataaagaga caaaaacaaa agtagcccgt aagggtggaa aagcatctca aagtacggga
     6661 aaaaataaaa aatagataga tagatagaag aattttatct tctattgaat ttttttgatt
     6721 aataaaaaat tttttacgat tttgttcgcc taaaattgta gtagttgagg taacatctac
     6781 gataatggaa gagttatgat actagtgcaa tggctgtatc tgaattctat tatatctatt
     6841 atcatagtat ctggagtttg agataagtca tggacgactg ccaatgacta gtgaattcat
     6901 taatacttct catttgatga tttttttata aattactttc aacaataact aatatagagt
     6961 taatatttac atttagtaaa cttgtatacg cttgctgcca gttattaata taaattatca
     7021 ttaattatat cgattaatga taaaagctat atcttggaat taattctttc ttcacaagaa
     7081 caataaatag agaaaggact acaatttctg agatttgggc tagagcagta gaatgaagca
     7141 acattaatgg ttatagatga tttatcttaa agagaaagtt agaaatgcaa tactaaataa
     7201 ttgaaagatt ttcgcagatg actctataaa actagaagaa aatgaactat aactattaaa
     7261 atttctacag atttttattt tacaaatgat acattaataa cgaaaacaca atcaatcaga
     7321 caatactctg atgccttcag taaatcctac aaaaaattaa ttatctttaa taaatgcact
     7381 cactaactaa aactattttt tatttggatc tttattcatt tctttataaa atcaagatag
     7441 gatttgctag acattatata attaaaccaa agcctatggc ataattctta tcaaaataat
     7501 ccaacttcca tactggtctt cttgtattga atgaagaaat acatttgatc aaactaaaaa
     7561 aataatcaaa gtagtaactt tagtagacaa atcttcaagc tttgcttata ggctagtggg
     7621 agctttcaga cataagaaaa tttatatcac caatttaagc actagaaaat aaatttcctg
     7681 aaatgtaaaa aattaaacta atcagcttag agaataaaat taaaaaaagg atcattatac
     7741 cagaaagatc tgaatcgaat ccttgatgtt tctaattaaa aagagattta atacatagtc
     7801 aatttgtact ttactgacag atggaaagga aggaaggaat aattattgat gaagacgact
     7861 ttttctctag aaagagaaaa gagataggta ttatctcagt agttgcaata attatttctt
     7921 catttggctt atttttttat caacagaata ttacagaaca aaacattaaa gatagtatat
     7981 ttaaccagta taaagaccgt caaatagaaa ctacacaaat tatttctgaa agcattggat
     8041 ctgacctgaa attgattatg tcaatccttc agggaatagc agattctgtt cctctacaac
     8101 aaggtgaact atatggagat agaattcatc agcttatgaa agaaaaattt gatcaaatta
     8161 atgatattac taaagttgat gggttgttta ttgctgatga agataatata ataacacata
     8221 atatagtatc tgaaggaaaa agaagttttg ttaatataga tatttcattt agagattatg
     8281 tacaagagac aagaaatact ctaaaaccaa ctttttcaga tggatttaag ggaattgata
     8341 atatatttag tattgcatta actgttccaa tagtaaatag tgatagtgga aaatatatag
     8401 gaataattgg gatacagata cctactgagc ctttttttgc acattctgga aatattaaca
     8461 atgttgattc acagttttta gtagcgtatg atagtaaaaa aaattacctc gccactccta
     8521 ggacccaatt cataggaatg aatttttttg ataacgatgt ccaaaatttt tttaatgtaa
     8581 cagataccca aaataattat tatcgtaaag tcttttccgg taaattatta ggaggatctt
     8641 cagtatatga ttttggaagt ggtgagagac taaatacagg ctacccaatt ttgttacaag
     8701 aaaaacctga atattttata ttcgtaataa cacctactac acaaatatat tcccatatca
     8761 atgatattct tcatacggaa agagtaaaag gtttttcgtt gatttttggc ctttcatttg
     8821 cagttctgat tttaatcata ttttttataa agtggaataa tacattaaat aatgaagtaa
     8881 gaaaaag
//
LOCUS       DASVEV010000031         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1171866, whole genome shotgun sequence.
ACCESSION   DASVEV010000031 DASVEV010000000
VERSION     DASVEV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171866"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..610
                     /locus_tag="VF222_01655"
     CDS             131..610
                     /locus_tag="VF222_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141161.1"
                     /translation="MSNKTIFVVMSLFAVGMIFSPLAVSAFPNQGNFLNLKKAVIDIN
                     DKVITDIIFKAQGDIPKHNPGIDWGYGIITVDESGIQNVIATTSHPGLPIADSELQQS
                     AADPVIHNHYVILGTDELCGVNPMRGFNLSVADLTHESPGRIFVKQNEAILKTFQNQ"
     gene            703..912
                     /locus_tag="VF222_01660"
     CDS             703..912
                     /locus_tag="VF222_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141162.1"
                     /translation="MDDTNFAVCVVDVRALDPQDHNTVIIGKKDFYGYNERPYDNYGE
                     NGYPEENNYGANGYPEENNYGENGY"
     gene            complement(1022..1144)
                     /locus_tag="VF222_01665"
     CDS             complement(1022..1144)
                     /locus_tag="VF222_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141163.1"
                     /translation="MLIEENENRLDLLNTEIQQQSYVIPESNKIKILRLVFINK"
     gene            complement(1299..1922)
                     /locus_tag="VF222_01670"
     CDS             complement(1299..1922)
                     /locus_tag="VF222_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEX7141164.1"
                     /translation="MNNNYTAIIELQNEYAKKVITHDYLNNFDTVCGIDVSYKGSNAF
                     CSAVIINKITLKSIEIVNEKSTVKYPYIPGLFMLREAEPLLKILRLLKNSFDVLLIDG
                     HGILHPRKCGLACYIGIIIDKPTIGVAKNLLCGSTLENNYIEYNKKILGYRIRKHNKK
                     DIYISIGYKISLETAVSIVRNLTKKREFIPEPLRIADTNSKKYSNFK"
     gene            complement(2355..2798)
                     /locus_tag="VF222_01675"
     CDS             complement(2355..2798)
                     /locus_tag="VF222_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141165.1"
                     /translation="MLKRAEKIMAKDVINILKQNLQEKESMYDKIRTFESKMLNDRGE
                     NNNHPDSFKLGSAVADMLTYWNSQKSPSKVYVLNRRVHHGAIGALLGLSTLYKNTPIA
                     TDILSGLGVGLQKDDYNDSKECSYLRKKKMRQKKINNLTSIAFEN"
BASE COUNT         1185 a          487 c          480 g         1254 t
ORIGIN      
        1 aaattagtta aataatattt tttatttatc ttaagtagta tagctatttt ttttcgaata
       61 ttatgtagta taataataat tgtgtagtat ataacgtcac atataaaaag gttataaaaa
      121 aatagtacat atgtcgaata aaacgatatt tgtagtgatg tctctctttg cagtcggaat
      181 gattttttct ccactggctg tctctgcatt tccaaaccaa ggcaattttt taaatctaaa
      241 aaaagcagta atagacataa atgataaagt aataacagat ataattttca aagcacaagg
      301 agatattcct aaacacaatc caggaattga ttggggatat ggaattataa cagtagatga
      361 atcaggtata caaaatgtga ttgcaacgac ttcacatcct ggacttccaa ttgcagatag
      421 tgaattgcaa caaagtgctg ctgatcctgt tattcataat cactatgtaa tattaggaac
      481 ggatgaacta tgtggagtta atcctatgcg gggatttaat ctttctgtag ccgatcttac
      541 acatgaatca cctggtagga tatttgttaa acaaaatgaa gcaatattaa aaacctttca
      601 aaatcagtaa ctggaggtaa ttttaaccag agtctagtct tcactcccgg taccgattat
      661 cgagctgcag catcattcca attagaatat gttgaagatg aaatggatga tacgaatttc
      721 gcagtttgtg ttgtagatgt tagagcatta gatccacagg atcataatac ggtaataata
      781 ggaaagaaag atttctacgg ttataatgaa agaccatacg ataactatgg tgaaaatggc
      841 taccctgaag aaaataacta tggtgcaaat ggctaccctg aagaaaataa ctatggtgaa
      901 aatggctact gagatgaatc atatagtact tattattaaa tgaaagttct gaaaatagag
      961 aacttaaaag atccaaactt ttatttattc tttttctatg tactattatt atttataaaa
     1021 tttatttatt aatgaatact aatcgaagaa tttttatttt atttgattca ggtattacat
     1081 atgattgttg ctgtatttct gtatttaaca aatcgagcct attttcattt tcttcaatta
     1141 acatagtaga tgtccagtta gattttccaa attcgccata aaagattgtt gtttttgttt
     1201 ctcgtattct ttcataaaag aagattattt tggatttaat ccattattag aaatgaaatt
     1261 ctaaagaatg ctatggtata aatttaatat cgcatcttct atttaaaatt actatatttt
     1321 ttagaattag tatctgctat ccttaaaggc tctggaataa attcacgttt ttttgttagg
     1381 tttctaacaa tgctaactgc agtttctaga ctgattttat atcctatact gatatagatg
     1441 tcttttttat tatgctttct aattctatat cctagaattt ttttattgta ttctatgtaa
     1501 ttattttcta atgttgaacc acataataag ttttttgcaa cccctattgt tggtttgtca
     1561 attattatgc ctatatagca agccaatcca cattttcttg gatgtaagat tccatgacca
     1621 tcaattagta gtacatcaaa tgagtttttt aataacctta agatttttag taaaggttcc
     1681 gcttctctta acataaaaag ccctgggata tagggatatt tcacggtact tttttcattc
     1741 actatctcaa ttgatttaag ggtaatttta ttaattatta cagcagaaca aaaagcatta
     1801 gaacccttat aagaaacatc tataccacaa acagtatcaa aattatttaa ataatcatga
     1861 gtgatgacct tcttagcata ttcattctga agttcaataa ttgctgtata attattgttc
     1921 atttttcttt tgtaataggg tataatctgc taaattaatg aagttttata aaaagccgac
     1981 tattagatag tagaaccagc gaaaaatagt aatgtagtat ttcctacaaa aaagatagaa
     2041 ccctctttta aatttgataa agaattggta gtagaaggta tgacctcact tgacatgtta
     2101 aaaatatata ataaaatatt taacaatatg aattggtttt attaacaaat atcctttctt
     2161 tctcatcaga atttgaggat aattataaag ttgatgtatt aaagttgtga acaatacggt
     2221 agatgtagta ttaaaattaa ccttttgaag tgagtagatg tagccatcta acaagagaga
     2281 caagaaagat atgatatctc gcataaaaat taactaaata aatcaattat ggtcctcatc
     2341 aatgtctatt actcttaatt ttcaaatgct atactcgtta aattatttat tttcttttgc
     2401 ctcatcttct tttttcttaa ataagaacat tctttggaat cattataatc atctttttga
     2461 agaccgacac ccaaccctga caaaatatca gtagcaattg gagtattttt gtataaagta
     2521 gataaaccga gcaaagcacc aattgctccg tgatgcactc ttctattcaa cacatatact
     2581 ttagatgggc ttttttgtga attccaataa gtcaacatat cagcaactgc agatcctaac
     2641 ttaaatgaat caggatgatt attattttct cctctgtcat taagcatttt agattcaaaa
     2701 gttcttattt tatcatacat tgattctttt tcctgcaggt tttgttttag tatgtttatt
     2761 acatctttag ccattatttt ttcagcccgt tttaacattg ttttatatgc taatatctca
     2821 tcatttttct aaatattcta aaactacata tcgacaaagt tgtatttaga agaatttaag
     2881 aaatgatgtg ccagtcctta tccagtctga ctcactttga cttgatttag tttgctcaag
     2941 tattatttac tttaactaaa ataatttcta ggctatatcc aaagagaatg ttagaatatt
     3001 aaatagatct gaatttctat tgtgatacta tgagaaactt taagattagt ctatatctgc
     3061 tatattagga aattactttt agcccgctat atgcattatt ctcaaaggct ccctaattta
     3121 tcacataaag tttagatacc taattatcac aaaattaaga tgaacctcaa aataatagat
     3181 agaatttact caacatcggc ttgtgtattt atgagaaggt taaaaagttt ataaactgga
     3241 atatataata aattctgaaa taaagttaca aaaataatgt tatactttta gtttagccca
     3301 ttccctttcc tttatcttta ctatgtagac actatgttga actgcaacta ttgtatcaaa
     3361 aaatgatgtt ttagttaaag agtcagccca aatatcttta aaaata
//
LOCUS       DASVEV010000032         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1366579, whole genome shotgun sequence.
ACCESSION   DASVEV010000032 DASVEV010000000
VERSION     DASVEV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366579"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1194)
                     /locus_tag="VF222_01680"
     CDS             complement(367..1194)
                     /locus_tag="VF222_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX7141166.1"
                     /translation="MIEITLGHTPDADDAFMFYGLASENVESNEFHITHVIEDIEKLN
                     KRALNNELDITAISVYAYAFLKDYVILRSGGSFGINYGPIILAKEKFLKYDLNKKIIA
                     IPGKMTSAYLLLSLLLGNFKEKELPFHTIPQAIIDNQVDAGLVIHEIQLTYKSLNLHK
                     IIDLGSWWDKLTNGLPVPLGINVANKKTLTLEQIRRFDSLFKNSIIYGLNNMEEALDY
                     AMKYGRGQSKDMISKFVKMYVNEITVDMGEKGKTSIERIFQLASERNIISPFQIDFA"
     gene            1328..1624
                     /locus_tag="VF222_01685"
     CDS             1328..1624
                     /locus_tag="VF222_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141167.1"
                     /translation="MNNKNFIFQQLKWISIYFAISIVISFIIPFPYSIIVIIPIVLFL
                     SFYIRRREMRKFGGGVENFSMFNSNKVNYYCMVCGTKHDNLTCPKCGSKMKRIG"
     gene            1798..2196
                     /locus_tag="VF222_01690"
     CDS             1798..2196
                     /locus_tag="VF222_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141168.1"
                     /translation="MFRKEIYLPFLLSILLFLSIDISFYKYIVLGKEDDSKDNVKEEK
                     DERDDNRDSKDNVKEEKDERDDNRDSKDNVKEEKEEDFEDISLDELFEDNNADDNRDS
                     KDNVKEEGKDEKEAEDEVPFLLPFNAVPFP"
BASE COUNT          915 a          293 c          355 g          827 t
ORIGIN      
        1 tacagctttt tcagaagaaa tatttcgtgc tgcgggaaaa gctagtggta tatcaataca
       61 ggaacttgta aatttgatca aagaaataaa aagagaacct gctcaacgag atacattttt
      121 taaagtaata gaaaaatact aatagttata caaatcaaca acatatcaga aaattacaaa
      181 atcgacataa actattactt ttttatcata atattctatt ttataaatta atgaactgta
      241 aaactatatg ctcatactac attctcataa ataaatcctt acgatggaaa tttcaagaat
      301 agttctgatt gtaattaatt gaataaagat atatatctta aagttggttt tataaatttc
      361 tataaattag gcaaaatcga tttgaaatgg agatataata tttctttcag atgccaattg
      421 aaagattcgt tcaatagatg tctttccctt ttcacccata tcaacagtga tttcgttcac
      481 atacatttta acaaatttag atatcatatc ttttgattga cctcttccat acttcatggc
      541 ataatctaaa gcttcttcca tgttatttaa cccataaatt atagaatttt taaacaagga
      601 gtcaaagcgt ctaatctgtt ctaatgttaa tgttttctta tttgcaacat ttatacctaa
      661 tgggacgggc aaaccgttag taagcttatc ccaccaagat cctaaatcta taattttgtg
      721 tagatttaat gacttgtaag taagttgaat ttcatgaatt actaaaccag catcaacttg
      781 attgtctatt attgcttgag gtatagtatg aaaaggtaat tctttttctt taaaattacc
      841 taacaaaaga gataataata aatacgctga agtcatcttt ccaggtatag ctattatctt
      901 tttatttaaa tcatatttta aaaacttctc ttttgctaaa ataattggac cgtaatttat
      961 tccaaaactt cctccacttc gaagaattac ataatcctta agaaaagcat aggcataaac
     1021 agatatagct gttatgtcta actcattatt caatgcacgt ttattgagct tttcaatatc
     1081 ttcaattaca tgagtaatat gaaattcatt agactctaca ttttcacttg ctaatccata
     1141 aaacataaaa gcatcatcag catctggagt atgtcctaaa gttatttcaa tcatttgtag
     1201 tttttagata taatactaat cttttcgtta tattaattaa ttgtttgatg agatattaaa
     1261 tcaaaaaaaa atttcaataa ttaaaagtag atcaagattt atttacatga aatatataat
     1321 atagttattg aataataaaa attttatttt tcaacaatta aaatggatat caatatattt
     1381 tgcaattagt attgtaattt cttttataat tccattccca tattcaatta ttgtaataat
     1441 tccaatagta ttatttttga gcttttacat aagaagaaga gaaatgagaa aatttggagg
     1501 cggagtggag aacttttcca tgtttaattc taacaaggtt aactattact gtatggtctg
     1561 tggaactaag catgataatt taacgtgtcc aaaatgtggc tcgaaaatga aaagaatagg
     1621 ataataatta tacatttttc atacacctca aaataaaatt ttgtttatac aaatacaaac
     1681 tatctaatga tagtttcata tagaaattat ttctattaaa tatacctgta ttattatcta
     1741 gtaatacgtt acttaaagct aatataattt aaaataacat gagtaatagt actacaattg
     1801 tttagaaaag aaatatacct tccattttta ctaagtattt tactattttt aagtattgat
     1861 atttcatttt ataaatatat tgttctagga aaggaagacg atagtaagga taatgtaaag
     1921 gaagaaaagg acgagagaga tgataataga gatagtaagg ataatgtaaa ggaagaaaag
     1981 gacgagagag atgataatag agatagtaag gataatgtaa aggaagaaaa ggaagaggat
     2041 tttgaagata tttctctaga tgaattgttt gaagataata atgctgatga taatagagat
     2101 agtaaagata atgtaaagga agagggaaag gatgaaaagg aagcggaaga tgaagtgcca
     2161 tttttacttc cgtttaatgc agtaccattt ccataaattg tgaaattaaa attagttatt
     2221 atatattaag atgataaagg gagttatgtg ataaaaatca catttgtaaa gtttaatatc
     2281 agggttatca gacttgcata atgggcgggg gtcgtagagc ccggccaaat aacaaaaaat
     2341 ttggagacgc gggacttaag atctaagaat gggtgatccc gtcctttaga
//
LOCUS       DASVEV010000033        14164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1172153, whole genome shotgun sequence.
ACCESSION   DASVEV010000033 DASVEV010000000
VERSION     DASVEV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14164
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172153"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /gene="trpA"
                     /locus_tag="VF222_01695"
     CDS             <1..496
                     /gene="trpA"
                     /locus_tag="VF222_01695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7141169.1"
                     /translation="ILYRKGYIQFAEKAKESGIDGFIIPDIPIEESKEYLNTMQNIGM
                     STIFLVSPNTSKKRLKRISRICTGFLYAISIYGTTGERKGFDEYTIESIKRVKKVTAD
                     KIPLAVGFGISNSQHIKYMIDAGADAVIIGSAIIKKIKEIETNELLYNSLNRFIGELK
                     SSCK"
     gene            complement(532..858)
                     /locus_tag="VF222_01700"
     CDS             complement(532..858)
                     /locus_tag="VF222_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141170.1"
                     /translation="MSVCDFVLACSIDDSPSYFTYDNDDTMIIIQSKSSALASNSDFC
                     YIEPYIELLISHESVHFVIKKIEGSLDSEMLDSLEVIIHRNAKNYQVTINNMLFASDN
                     TGLVLE"
     gene            complement(982..3588)
                     /locus_tag="VF222_01705"
     CDS             complement(982..3588)
                     /locus_tag="VF222_01705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7141171.1"
                     /translation="MAKIPRISSALNAVDIEQNPPPLIIGERVNSQGSRKAKEMVLKD
                     DFEGLLNLARTQIEEGAHCLDICVATTERSDEHDFMCQLVKRLCLEIEAPLVIDSTDP
                     KVIESAIKQIPGRPIINSINLEGDGSRFHSLAPLMIKYGVPAVAMCIGPNGMAKTPKE
                     KLETAQLLYETGKKYGLKPWQFIFDVLTFTLATGQEEFLDSAKNTLEGIKLVKENLPG
                     CFTTLGLSNVSFGLPTRARKIINSVFLYHAIKNGLDSVIINPKDIVAYTDISQEERKL
                     AENLIFNLRNTALSDLISYYENETNTDQTNKKRSDGGNSKLDIDPNWKFDKKCYYRIV
                     NRLREGIEYDVVGAILDNVKDKDTKKVELSSLKKSTSSSSSSSSSSSSLLLNDDDVTA
                     LLSKEELHEAAVKTLNETLLPAMKEVGDKFGAGEIILPFVLKSAECMKAAVAELEKYL
                     IKKKDVSKGRIVLCTVYGDVHDIGKNLVKTILVNNGYSVYDLGKQVQLQTIVNKISEV
                     NADAVGLSALLVSTSKQMQIFVDYAKEHNIQIPILCGGAAINTNYINRIAKNGGLYEQ
                     GVFYCKTAFDGLNVMNNLMSDKRIQYISHWKEKVELWKEFKPTSINVSQTANKHSGIK
                     PVLSPPIPPSIGLQIRLGLEDINLEDVWNFINKKSLFTLSWSFRGKAASGLSEEAEKL
                     LIEWKKRVIDENLFSPKVVYGYFRCHNRNNKLVVDSSKGNEVIFDFPRSTKGRNLCLT
                     DYFGENDIVAFQSVTVGKKASYTIEKWNEENKYTDAYYLHGLAVETAEALADWTNSLI
                     RKELKIDSKRGLRYSWGYPSCPDIAQHKLVWELLDPAKSDMTLTEAGQIVPDQSTAAI
                     VIHHPEAEYFTL"
     gene            complement(3723..4670)
                     /locus_tag="VF222_01710"
     CDS             complement(3723..4670)
                     /locus_tag="VF222_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7141172.1"
                     /translation="MKKESFLEALKKRVLLCDGAMGTEIQKLDPQSQDFPDNKDGFND
                     GLILSRPDWIKQIHRNYLNAGADCIETNTFGGNKFKLDEYGFGDQTIEFNKKAAELAR
                     QVCNEFQDKTHFVIGSIGPSGFLPSSNDPDLGQISLEKIVNAFALQAEGLILGQVDAL
                     LIETSQDILEVKLVIEACHNAMEKVQKEIPIIANITLDQYGKMLLGTNVQSAYTTVSD
                     MGINIFGLNCSTGPDEMTSSVIWLDEQQDLPILVVPNAGMPHNEGGKAVYKMTPDKLA
                     HKLEEFILKYKNIKVIGGCCGTTPEHIAELRKMIDKIQE"
     gene            complement(5453..5761)
                     /locus_tag="VF222_01715"
     CDS             complement(5453..5761)
                     /locus_tag="VF222_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141173.1"
                     /translation="MSNFNTTSNQNNNIYPRPCVYNCGVQIYWNSSISEYWEVFTKKK
                     HICPNRSIKSSGTKSTTAKGGGITPPLDSPKPRYYNPSAKKRTPYASYFATTSKPNII
                     "
     gene            complement(5748..6002)
                     /locus_tag="VF222_01720"
     CDS             complement(5748..6002)
                     /locus_tag="VF222_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141174.1"
                     /translation="MSSSSNNKKVLYQLWTNGKGSFAVGKIGSQLFEYSDSQYSYDKI
                     LDSKMLYDGSITEELVQDWIQELKQEDILDEDGNLIYVKF"
     gene            complement(6022..6447)
                     /locus_tag="VF222_01725"
     CDS             complement(6022..6447)
                     /locus_tag="VF222_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141175.1"
                     /translation="MSSTTKNNLKNKNILIVDDDIETSSKYKRWLEEDGGFNLTLIND
                     PDLAEQNFNPESYVLVLIGFRMSVIDGFDLYNKLHEMSKILQRDTSPLNDFRVCFMTS
                     SRINYEALSEIHPEFGEECYVCKEVAKDVFIKHVYSLIS"
     gene            complement(6664..7137)
                     /locus_tag="VF222_01730"
     CDS             complement(6664..7137)
                     /locus_tag="VF222_01730"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7141176.1"
                     /translation="MKILNAGPGFSQPMTEDETISFLTSGRRNIYIASLDEKNEPNIH
                     PVWFYFDPSNQKFYIETSKYSKKKKNLETNKIIYFCVDEPAIPYKGVRGKAKVNIISD
                     IDSIVPICERNMKKYLGSLEHPMAKELVNLAKRGESVAIEISPLYFSTWDDSKIK"
     gene            complement(7661..8734)
                     /locus_tag="VF222_01735"
     CDS             complement(7661..8734)
                     /locus_tag="VF222_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141177.1"
                     /translation="MESILEQYNSKLYSLQEKNVISREQLDHFLHRGLEIINPSSNQF
                     NLLSLAKFLPYIIIGASKDYIDKLNIPYQATSYVAAESSPNSDRYLYKIPLKKGGGVR
                     QLTGGKRKFGDNLYASTHKIIMGYQAVLSSIYNLVQNKQQTLDWKFYANHFQQSSPEL
                     YEDLCKLNDIYSRQQTGSPSHPYFIIVARHAKSFQRSGLIELYLKNQITIFNQTLRKI
                     IFLTDQSGYDYCSKVIKESDNIKYATTGDKFNIWNGLSNLKKKYKISLILNDGGRIMS
                     NGFKENGVIAEERVTLESYPGKEFLDFDHKDHLLQGCTLGIKGSGLDGSELKGGLLAH
                     SIKINDEEKANVYVYPLYDKGAT"
     gene            8950..9915
                     /locus_tag="VF222_01740"
     CDS             8950..9915
                     /locus_tag="VF222_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7141178.1"
                     /translation="MTTTTKFRIATVHDKDYVLNFCKNTFSWGDYIDRVWDIWIGEPN
                     SIFLVAVVVNENNIEIPIAIAHGILMPEKTVWVEGIRVDPKYRSQKLATNMSLSILDY
                     ARKKGALYSSAIVSINNEASKGLMEKLGFKVISKWSYISIKPIILPELNNNNLIVCNS
                     TKVANQNEYQQILNFLNKSDIFKASGKKFVNSWRWYDLTDDRLRMMINNKQVIIVVND
                     NVDKKEQKIRGIAIIEREGYWNNQNIFQIVYIDADSDEILLLLVIKSLEFTFSNGNKK
                     EKKYVENKYERMQIFSPYTIKDNSLIFQTFNASFSEQFLLYHKEI"
     gene            complement(10118..11335)
                     /locus_tag="VF222_01745"
     CDS             complement(10118..11335)
                     /locus_tag="VF222_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141179.1"
                     /translation="MSSYSDVKKQLEKYWFNYLNENAKRFSRSTISGASPPSVFVGRY
                     GYPKVSIGPMVPDDIHGNTTILDAPEMWLGKTLQDILNYRLSLVRGLNFIKINDLDNK
                     FILSLQELTMSTKPAESELNFHKPPTFHQSLLSQNNDLNKEFTPFGPQAEIKSFKLSS
                     NTSTNKKIDNLYSDNTVKATDAVIDLYNNGIEISQINKVLSLGMLGRKNKRHLVPTRW
                     SISATDDIISSYLVRKIEFYQSIDKFEVIEYNHFSNYYSIILIPSDVWSFEMVEAWYD
                     QNNVNKFFLESDYESATGLDHYPRIAGAYFAAKLGMLEYLYSCRKRKCSVLVLREIRP
                     EYLVPLGVWQIREGIRDALRSKQVPKTNSFSDFRKALNYASKGMKVPLLDWLKHSEIY
                     KTYGKKTLISDFF"
     gene            12003..12710
                     /locus_tag="VF222_01750"
     CDS             12003..12710
                     /locus_tag="VF222_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141180.1"
                     /translation="MRKYLERDPETEIGKLFNVSVAHVSSIVKEESKKDGYILVIREV
                     VKMFKRDNLEIGDVISGIRLKNQVKEVGLTISFFEDFLEATNTESFRLGMDHEKFLEI
                     VKRMLHFEKIFKIKLEEIPDFSHNAIKEFTRVTGEISKAETKLTQIYDNYGVKKSEVE
                     RYLEEKSLFVKSKLVDVALPTHYDWMVISDSKFKKASKITKTKIDPKTLYKKLNSVYK
                     EPDKHIDIVKQIMNSND"
     gene            13022..14101
                     /locus_tag="VF222_01755"
     CDS             13022..14101
                     /locus_tag="VF222_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7141181.1"
                     /translation="MISIKNLTKKFDKIVAVDNVSLDIREGEVFGLLGQNGAGKTTTI
                     HMLATLIKPTSGTALINGYDIIKNSSKVRKNIGIVFQAPSSDDMLTGFENLKIHSYLY
                     GVPSELRIKRINELLKLVGLYERKDDIVKKYSGGMRRRLEIARGLIHKPKVMFLDEPT
                     LGLDPNSRKSMWEYIQKLVKEEKITIILTTHYMEEADTLCDRIGFINKGKIIAIDTPQ
                     QLKLMIKSNNNNNNNNNVNNSADKYIGDIIKLYFKVTEDSDGISNNNSHILTKNIEIL
                     KSFKFVKKVIENKEIGSKNLVLVLHVDDINHNLPIILKSIDNVESIEFSQPTLDDVFF
                     TFTQGKIAEEPEGGFMERYAQYDKK"
BASE COUNT         4909 a         2091 c         2054 g         5110 t
ORIGIN      
        1 tattttgtat agaaaaggat acattcaatt tgcagaaaag gctaaagaaa gtggtattga
       61 cggttttata attccagata ttccaataga ggaatctaaa gaatatctaa atacaatgca
      121 gaatatagga atgtctacaa tttttttagt atctcctaac acctcaaaaa agagattgaa
      181 gaggattagc agaatttgta ccggtttttt gtatgctata tcaatttatg gcacaactgg
      241 agaaagaaaa ggatttgatg aatataccat cgaatcaata aaaagagtca agaaagttac
      301 tgcagataag attcctttgg cggtagggtt tggcataagt aattctcaac acataaaata
      361 catgattgat gctggagcag atgcggtaat tataggaagt gcaattataa aaaaaataaa
      421 ggaaatcgag actaacgaat tactttataa ttcactaaat cgatttattg gtgaactgaa
      481 aagtagttgt aaatgatgat gttaataata ataataaatt atctaggacc attattctaa
      541 tactaaacct gtgttatcag aagcaaaaag catattgttt attgtaactt gatagttttt
      601 tgcatttcta tggattatta cctcaaggct atctagcatt tccgagtcaa gagaaccttc
      661 tatctttttt ataacaaaat gaactgattc atgggatatt agtaattcta tatatggttc
      721 gatataacaa aaatctgaat tacttgcaag agcacttgat tttgattgaa taattatcat
      781 ggtatcatca ttatcatatg taaagtagct tggagaatca tctatagaac atgccagtac
      841 aaaatcacaa acagacatta aattatcatt ataattctaa tagattattt tatacatcta
      901 tcgcttagaa atctaatcta aagatgacct atcatctatt ttataagaaa tcaatacagc
      961 aacagcagta aataaataaa tttagagtgt aaaatattct gcttcgggat gatgaataac
     1021 aatggcagct gttgactgat cgggaactat ttgtccagct tctgttaatg tcatatcgga
     1081 tttagcagga tctaaaagtt cccatactaa tttatgttga gcaatatcag gacaacttgg
     1141 atatccccaa ctgtatcgta atcctctttt tgagtcaatc tttaattcct ttcgaattaa
     1201 agagtttgtc caatcagcta atgcttcggc agtttcaaca gccagtccat gcagatagta
     1261 tgcatccgta tatttgtttt cttcattcca tttttcaatt gtataagatg ctttcttacc
     1321 aacggtgacg gattgaaatg caactatatc attttctcca aaataatcag tcaagcacaa
     1381 attccttcct tttgtagaac gaggaaaatc aaaaataact tcgttgcctt tggaagaatc
     1441 tacaactagc ttattatttc gattgtgaca gcgaaaatat ccataaacta ccttgggtga
     1501 aaaaagattt tcatcgataa ctcttttttt ccattcaatc agtaattttt ctgcttcttc
     1561 tgataagcct gatgcagctt ttcctctaaa actccacgat aaagtaaaaa gtgacttttt
     1621 atttataaaa ttccatacat cctctaaatt tatatcttca agacctaatc gaatttgaag
     1681 acctatgctc ggtggaatgg ggggtgatag aaccggttta ataccactat gtttattggc
     1741 ggtctgagag acatttattg aggtcggttt gaactctttc catagctcta ctttttcttt
     1801 ccagtgggaa atatattgaa tccttttatc tgacattaaa ttgttcatta cattcagacc
     1861 atcaaaagca gttttacaat agaaaacacc ttgttcatag agtcctccgt tctttgctat
     1921 tctatttata tagttagtat ttatagcagc acctccacaa agtattggta tttgtatatt
     1981 atgttcttta gcataatcaa caaatatttg catttgtttt gacgtagaaa caagcaaggc
     2041 cgacaatccc acggcatcag catttacttc actaatttta ttaacaattg tttgcaattg
     2101 tacctgttta cctaaatcgt aaacagaata tccattattc actagaattg ttttaactag
     2161 attttttcct atatcatgaa catcaccata tacagtacat aatacaattc ttccttttga
     2221 aacatctttc tttttaataa gatatttttc taattcagct acagcagcct tcatgcattc
     2281 cgcagatttg agaacaaaag ggagaattat ttcacctgct ccaaatttat cacctacttc
     2341 cttcattgca ggaagtaatg tttcgttcaa agtctttact gctgcttcat gtaattcttc
     2401 cttagataaa agagcagtaa catcatcgtc atttaataac aaagaagagg aggaggagga
     2461 ggaggaggag gaggacgatg ttgatttctt taaggaggac agttcaactt tttttgtgtc
     2521 cttatcttta acattatcca aaattgcacc tacaacatca tattctattc cttcacgaag
     2581 tctatttact attctatagt aacatttttt atcaaatttc caatttggat cgatatcaag
     2641 ttttgaattt ccaccatctg atcgtttttt atttgtttgg tcggtatttg tttcattttc
     2701 ataataagaa attaaatctg acaaggctgt atttctaagg ttaaagatta aattttctgc
     2761 aagttttctt tcctcttggc tgatatctgt ataggcaact atatcctttg gattaataat
     2821 tacggaatcc aaaccatttt ttattgcatg gtatagaaat acagaattga ttatttttct
     2881 tgcacgtgtg ggtagaccaa agctaacatt acttaagccc aaagtagtga aacaaccagg
     2941 aagattttcc tttactagtt taattccctc taaagtattt tttgcagaat ctagaaattc
     3001 ttcttgaccc gtagccaaag taaaagttag aacatcgaat ataaattgcc aaggttttag
     3061 tccgtatttt ttgcctgttt catacaagag ttgtgcagtt tccagttttt cttttggagt
     3121 tttagccatt ccattaggtc caatacacat tgcaacagca ggaactccat atttgatcat
     3181 aagaggagct agggaatgaa atcgacttcc atcaccttcc aagttgattg aattgattat
     3241 tggtcttcct ggtatttgtt taattgcgga ttctataact ttagggtccg ttgaatctat
     3301 taccagagga gcttctattt ctaaacacag tcgttttact agttggcaca tgaaatcatg
     3361 ttcatcagat ctctcagtgg ttgccacaca tatatccaaa caatgtgctc cttcttctat
     3421 ttgcgttctg gcaagattga gaagaccttc aaagtcatct tttaaaacca tctcctttgc
     3481 ttttcgtgag ccttgactgt tgactcgctc tcctattatt aaaggaggag gattttgttc
     3541 aatgtcaaca gcatttaacg ctgaactaat ccttggaatt ttggccataa ttcattcatt
     3601 cattcctata attttttttg tgatcatagt cacaggaaaa tttacaaaat aaatatgtat
     3661 agtaatttta tcctattgta tcaatcaata ttatgattag cgattattta ttcttaattt
     3721 atttattctt gaattttgtc aatcatcttc cgtaattctg caatatgctc tggagtggtt
     3781 ccacaacaac ctccaattac cttgatattc ttgtacttta aaatgaattc ctctagttta
     3841 tgtgctaatt tatctggtgt cattttgtaa actgcttttc caccttcgtt atgaggcata
     3901 ccagcattag gaactacaag aataggtaaa tcttgttgtt catctaacca aataacacta
     3961 gaagtcattt catctggtcc tgtagaacaa tttagaccaa aaatattgat tcccatatca
     4021 gaaactgttg tatatgcaga ttgaacattc gttccaagaa gcatttttcc atattgatcc
     4081 aatgttatat ttgctataat aggtatttct ttttgaactt tttccattgc attatgacat
     4141 gcctctatca ctagttttac ttctaggata tcctgactag tttcaattaa aagtgcgtca
     4201 acttgtccca gaatcaaacc ttctgcttgg agagcaaaag cattcactat tttctctaaa
     4261 gatatttggc ccaaatcagg atcattggaa ctgggaagaa aacctgaagg tcctattgat
     4321 ccaattacaa aatgagtttt atcctgaaac tcattgcata cctgtctagc tagttctgct
     4381 gcttttttgt taaattcaat agtttgatct ccaaaaccat attcgtctaa tttgaatttg
     4441 tttcctccaa aagtgtttgt ttcaatacaa tctgctccag cgtttagata atttctatgt
     4501 atttgcttta tccaatctgg tctgcttaat attagcccgt cattaaagcc atctttatta
     4561 tcgggaaaat cttgagattg tggatctaat ttttggattt cagtacccat tgcaccatca
     4621 caaagtaata ctcttttttt tagcgcctca agaaaagatt cctttttcat ttttatttat
     4681 atatataata aatatttgaa tcttaattta aatatctcgc tttgcataaa ttctaaaata
     4741 gaaatataga agaggagaaa taataaatga aaattgcatc aaatttctaa tattataatg
     4801 tttaataaat acgatattct ctcaagaaaa acttcaacaa tgccatagcc tacatgatac
     4861 caaggtatag tgctatgaca attaaataaa caaaatttct caaattatta aatattatgt
     4921 tactaaatat gttacaataa ggtaatcaag agtcttaacc aaccacggcc agagttattc
     4981 tgtttgctcc atagtaatag atggccatgg ttttactttt aacattttta tctataaaaa
     5041 gtagtaattt tccattataa atttatgatt ccagtgaatg aaacaaaact gtatatgaat
     5101 cgtataaagc aagcgacgaa tcagataaat taagatagta acattattta caatatacag
     5161 tactttttat tggttctttc agtgaaatac catacatatc ctttatttta gtaaacaaaa
     5221 aaatgatagc attttttatc cattgatatt ctttaaattt aataaaaatt gtttctaact
     5281 cactgttata taggaaattg atttcattac gaagtttttg atatcttcgt taagatcagt
     5341 taaaaatttc tttaattaac aatatatttt aattaattaa ttatctattc tatttttttt
     5401 atacataatc ttattattat tttgattcga taatgtaata tttttatata ttttatataa
     5461 tatttggttt agatgttgta gcaaaatagg aggcatatgg tgttcgtttt ttagcagagg
     5521 gattgtaata tcgaggtttt ggtgaatcta atggtggagt tattcctcct ccttttgctg
     5581 ttgttgattt agttcctgat gatttgatgc ttctattagg acaaatatgt ttcttctttg
     5641 taaacacttc ccagtattcg cttattgaag aattccagta aatttgaacg ccgcaattat
     5701 agacgcaagg tcttggataa atattattat tttgattaga agtagtgtta aaatttgaca
     5761 tagatcaggt ttccatcctc atctaaaata tcctcttgct tgagttcctg aatccaatcc
     5821 tgaactaatt cttcagttat tgaaccatca taaagcattt ttgaatctag tattttatca
     5881 taggaatatt gagaatctga atattcaaat aattgtgatc ctattttccc tactgcaaaa
     5941 ctacccttgc cattggtcca cagttgatat aatacttttt tattattact agaagaactc
     6001 atttcttatt attaatattt atcaagatat taacgaatat acatgtttta taaaaacgtc
     6061 ttttgcaact tctttacaaa catagcattc ttctccaaat tctggatgaa tctctgacaa
     6121 cgcttcataa tttattcttg atgaagtcat aaaacatact cgaaaatcgt ttaacggtga
     6181 tgtatctcgt tgtaatattt ttgacatttc atgtaacttg ttataaagat caaagccatc
     6241 aattactgac attctaaacc caataagcac aagaacatag ctttcaggat taaagttctg
     6301 ttctgctaaa tcagggtcgt ttattaaagt tagattgaaa cctccatctt cttctagcca
     6361 tcttttatat tttgaagaag tttcaatatc atcatcaact atcaagatgt ttttgttctt
     6421 gaggttgttt ttagttgttg aactcatttc ttgttagtat tcataagcct tctatgttaa
     6481 aaagacattt aaacatattt ggaataatta tttgcatggt accttttttc aacaaaataa
     6541 tgagaaataa atatgattga tgatgataaa atagaataaa aagtgtaggt taaagtagtg
     6601 gcgtctttca atctatctat caatctattc atttagttaa attcatagat gaggatatgg
     6661 ttttcatttt attttactat catcccaagt agaaaaatac aatggagaga tttctatcgc
     6721 tactgattct cctcttttag caagattaac taattctttt gccattggat gctctaaact
     6781 tccaagatat ttcttcatat tcctttcaca aattggaaca atactatcaa tatctgagat
     6841 aatgtttacc tttgcttttc ccctcactcc tttatagggt atagcgggct catctacaca
     6901 aaaatatatt attttattag tttctaaatt cttttttttc tttgaatatt tagatgtttc
     6961 aatgtagaat ttttgatttg atggatcaaa ataaaaccag acaggatgaa tatttggttc
     7021 attcttttca tccaaagaag caatgtagat atttcttctt ccagaagtaa gaaatgatat
     7081 ggtttcatcc tctgtcattg gttgactgaa acctggtcca gcattcaata ttttcattta
     7141 taactaacta tcttattgat aacaactatt attatttttc taatcactac tataatttat
     7201 ttaatagtta aatggtagtt taagcgatca catactccat tataaggagg taaaggatca
     7261 tctctacaaa aatataaggt attattattt aacatatttt gcaatttttt ttaatttttt
     7321 tgctgtttct acataaatct ttttgttctc aacatcaaag aaataccaga cagtatgaat
     7381 attggattca aattttttat ccactgtttc aatgtatatg ttacgttttt tgtgttgata
     7441 aaaaatgtat aactttctct tgagtcattg gaagaccaaa cccgggctag aattaagaat
     7501 tttcaatatc agtttacctc attttctata tataaaagat ttgacttact atagttagtt
     7561 cttttaaatc attaattaaa taattaataa ttaataagta atttcaaaca tactattctt
     7621 catcatcacc atctccaata gttataatat aattcaaatt ttatgttgca cctttatcat
     7681 aaagtggata aacatataca tttgcttttt cttcgtcgtt gattttgata gaatgtgcta
     7741 ataaaccgcc ttttaactca cttccgtcta gaccagatcc tttaatacca agagtacagc
     7801 cttgcaataa atgatcttta tgatcaaaat ccaaaaattc tttccctgga taagactcca
     7861 aagtaactcg ttcttcagct attacaccgt tttctttaaa tccatttgac attatccttc
     7921 ctccatcatt caatatgagg ctaattttgt actttttctt taaattagat aatccattcc
     7981 aaatgttaaa tttatctcct gtggtagcat attttatatt atcactttct ttgataactt
     8041 ttgaacaata atcatatcca ctttgatctg ttagaaaaat tatttttcta agagtttgat
     8101 tgaaaattgt aatttgattt tttagatata attcaattag tccagatctt tgaaaagatt
     8161 tggcatgtct agctacaatt ataaaatacg gatgagaagg agagccagtt tgttgccttg
     8221 aatatatatc atttaattta caaagatctt catataactc tggagaagat tgttggaaat
     8281 gatttgcata aaatttccaa tctaatgttt gttgtttatt ttgtaccaga ttgtatatgc
     8341 tagaaagaac tgcttgatat cccattatta tcttatgagt agaggcatat agattgtcac
     8401 caaattttct tttgcctcct gttagctgac gaactccacc tccttttttc agaggtattt
     8461 tatatagata tctatctgaa tttggagaag attctgccgc tacataacta gtagcctggt
     8521 atggaatatt taatttatca atataatcct ttgaagctcc aattattata tatggcaaaa
     8581 attttgctaa tgataataaa ttaaattgat tgctactagg atttattatt tctaatcctc
     8641 tgtgaaggaa atgatctaat tgttctcttg aaattacatt tttttcctgc aaggaatata
     8701 actttgaatt atattgttct aaaattgact ccatgtttga tctatatagt aatagcagaa
     8761 ataattttat ctctcattaa ttaatctaat ggacttcatt atgaattaca ataatgatca
     8821 ttttagataa ttaaattaac tatatcaatg aaaaaagatc tgtattttgc aatttttata
     8881 tacaacttta aattttggag agaaatttta aatatcaaaa atcattaact ataatcttca
     8941 ttaatacaca tgacgacgac gacgaaattc agaatagcca cagttcatga taaagattat
     9001 gttttaaact tttgtaaaaa tacattcagt tggggggatt atattgatag agtatgggat
     9061 atatggattg gggaacctaa tagtatattt ttagtagctg ttgttgtaaa tgaaaataat
     9121 atagaaatac caatagcaat cgctcatggg attctaatgc cagaaaaaac agtatgggtt
     9181 gaaggaataa gagttgaccc taaatataga tcacaaaaat tagctactaa tatgtcctta
     9241 tctatactag actatgcaag aaaaaaagga gctttatatt cttctgcaat agtttctatc
     9301 aacaatgaag catcaaaagg attgatggaa aaattaggat ttaaggttat ttcaaaatgg
     9361 tcatatatta gtataaaacc aataatttta ccagaattaa ataataataa tttgattgta
     9421 tgtaactcta ctaaggttgc aaatcaaaac gagtatcaac aaattcttaa tttcttgaat
     9481 aagtcagata tttttaaagc atcagggaaa aaatttgtaa attcttggag atggtatgat
     9541 ttaacagatg atagattaag aatgatgatc aataataaac aagtcataat agtagttaac
     9601 gataatgttg ataaaaaaga gcagaaaatt agaggaatag ccattattga gagagagggt
     9661 tattggaaca atcagaatat atttcaaatt gtgtatattg acgctgattc tgacgagata
     9721 ttattgttgt tagtgattaa gagtttggaa tttacatttt caaatggaaa taaaaaagaa
     9781 aaaaagtatg tggaaaataa atacgaaaga atgcagatat tcagtcctta taccataaaa
     9841 gataattctc taatatttca aacatttaat gctagttttt cagaacaatt tctgttatat
     9901 cacaaagaaa tttaaaataa taacttttta ttgaataaat aaaattgaaa tatcatagat
     9961 ttaaagtatt ttaagttgat ttaagtaaca aataattatt attttttata aaactcttac
    10021 acgaatcttc aaggtatcaa tagttcaaaa ttttataata ttcttactaa tatataattt
    10081 aaaatagata taatgtattc ttctaatacc tcttgtttta gaaaaaatca gagattaatg
    10141 ttttttttcc ataagtttta taaatttcac tatgttttag ccaatctaaa agaggaactt
    10201 tcattccttt ggaggcataa tttaatgctt ttctgaaatc tgaaaaagaa ttagttttag
    10261 gaacctgttt ggatctgagt gcgtctctta ttccctccct tatttgccaa actcctaatg
    10321 gtacaagata ttctggccta atctccctca atactaaaac tgaacatttt ctttttctac
    10381 atgaatataa atattctaac attccaagtt ttgccgcaaa ataagcgcct gctatcctag
    10441 ggtaatgatc taaaccagtg gcactctcat aatctgattc taaaaaaaac ttgtttacat
    10501 tattttggtc ataccaagct tcaaccattt caaaactcca tacatcacta ggaatcaaaa
    10561 ttatagaata ataattagaa aagtgattat attcaattac ttcgaattta tcaattgact
    10621 gataaaattc aatttttctg accagatatg aggatattat atcatcagta gcagaaatgc
    10681 tccatctagt cggcaccaaa tgtctcttgt tttttcttcc aagcatccct aagctcagga
    10741 ctttattaat ttgacttatt tcaataccat tattataaag atcaataaca gcatcagtag
    10801 ctttaacagt attatcagaa tacaagttat ctatcttttt atttgtgctt gtattagagg
    10861 acaatttaaa agatttaatt tcagcttgag gtccaaatgg agtaaattct ttattcaaat
    10921 cattattttg ggaaagtagt gattgatgaa atgttggtgg cttatgaaaa tttagttcac
    10981 tctccgctgg ctttgtagac atcgttaatt cttgtaacga aaggataaat ttattgtcta
    11041 aatcatttat ttttataaag tttaatcctc taactaaaga aaggcgataa tttaggatat
    11101 cttgtaatgt ttttcctaac cacatttctg gagcatctaa aatggtagta ttgccatgaa
    11161 tatcatcagg taccatgggt ccaatactta ccttgggata accataccgt cctacaaaaa
    11221 cagatggagg actagcgcct gaaattgttg atctagaaaa gcgttttgca ttttcgttaa
    11281 gatagttgaa ccaatatttt tcaagctgtt ttttgacgtc actgtaagag gacacaggct
    11341 aataataact aaatatctct cattattaat tgatttttag tacaattatc cacgtgatct
    11401 cttgataaac ttcgagatac atcaaataac agtgatttta cagatttgga atagctggat
    11461 taattttgat aatcagatga atctttacaa gtactttttt tcttttcttt aacatggcca
    11521 ttgtctatag gtttgatcca ctaataaaat gatattttca tcatttcaaa tacctttcaa
    11581 atgcttttca cgtaattatt ttataattat ttaataacgt tcaaatagtt ttcaaataac
    11641 tttcaaatac gccattttat gctcatatta aggggtctaa taaagtagtt acagtattaa
    11701 accagatact aaaatttatt aaaatattat tttaatctca gataatttaa cgagaatttg
    11761 tctttactag atgattatca ctggtaccag atttacagta catatactca ttcaaatcat
    11821 gagaactcta ataaaaatgt caagtttggg gactataatc tgtaaagtaa tatctaaacg
    11881 attataaaat tctgatagct ctaaaagtaa aatttattta ataaaagata gtaagttttt
    11941 taaattgatt tttttagctc ttttatatga gagctacccc agcccaggtg aaatcagaga
    12001 tcttgagaaa atacttagaa agagatcctg aaactgagat aggtaaatta ttcaatgtaa
    12061 gtgtcgcaca tgtatcatct attgtaaagg aggaatcaaa aaaagatgga tacattttag
    12121 ttattagaga agtagtaaaa atgttcaaga gggataattt agaaattggt gatgtaatat
    12181 ccggaattag acttaagaac caggtaaaag aagtaggatt aacaatttcg ttctttgaag
    12241 attttttaga ggctacaaat actgagagtt tcaggttagg aatggatcac gaaaaatttt
    12301 tagaaatagt aaaaagaatg ctacattttg aaaaaatttt caaaataaag ctagaagaga
    12361 taccagattt ttcacacaac gctatcaaag aatttacccg ggtaactggc gaaatttcga
    12421 aagccgagac aaaattaaca caaatttatg ataactatgg tgttaaaaag tcagaggtgg
    12481 agcgatatct tgaggaaaaa tcattatttg taaaatctaa attagtagat gttgctttgc
    12541 caactcatta cgattggatg gttatttctg atagtaaatt taagaaagca tcgaaaataa
    12601 cgaagaccaa aattgatcca aaaactctat ataaaaagct taattctgtt tataaagagc
    12661 ctgataaaca tatagatatt gtaaaacaaa taatgaattc aaatgattaa aagattacaa
    12721 ccaggtgttt tatatctcaa gatgatttat tagaaacaat ttatagattt tcagataaaa
    12781 catatttgcc aatttgttct cttattttta gacacttggt tcatgaaata atctaatata
    12841 tgataatgaa aagggatcca aatacttttt tgttgagaga ggttatatcg tagtatttgc
    12901 tactaaatag acttgtgtat gtaattaaca aatagtaact ataataagtt aaatatttct
    12961 agtaaagtca attaaaacaa ggttaataca gcagataact taaattcaat tactttatag
    13021 tttgatttcc attaaaaacc tcacaaaaaa atttgataaa atagtagctg tagataatgt
    13081 ttcattagat attcgtgagg gtgaggtttt tggattatta ggtcaaaatg gtgctggtaa
    13141 aactacaaca attcacatgt tggctactct tatcaaacca acaagtggca cagcactaat
    13201 caatggatat gatattataa aaaattcatc aaaagtaaga aaaaatatag ggatcgtatt
    13261 tcaggccccg agtagtgatg atatgttgac tggttttgaa aatctcaaaa ttcattctta
    13321 tctatatgga gttccatcag aattaagaat caaaagaatt aatgaattat tgaagttagt
    13381 tgggttatat gaaagaaaag atgatattgt aaagaaatac tcaggtggaa tgcgaagaag
    13441 attagaaatt gcccgaggtc tgattcataa acctaaagtc atgtttttag acgaaccaac
    13501 acttggcctt gacccaaata gtagaaaatc aatgtgggag tatattcaaa aattggttaa
    13561 agaagaaaaa attactataa tattaacaac ccattatatg gaagaagctg atactttatg
    13621 tgatagaatt ggctttatca acaaaggcaa aataattgct atagatactc cacaacaatt
    13681 aaagcttatg attaagagta acaataataa taataataat aataatgtta ataatagtgc
    13741 cgacaaatat attggtgata taattaagtt atactttaaa gtaacagaag atagcgatgg
    13801 tatatctaat aataatagtc atatattaac aaagaatatt gagattttaa aatcattcaa
    13861 atttgtcaag aaagtaatag agaacaaaga aattgggagc aaaaatctag tactggtatt
    13921 acatgtcgat gatattaatc acaatcttcc tataatatta aaatccattg ataatgtaga
    13981 gtctatcgaa ttttcacaac caactttaga tgatgtattt tttactttta ctcaaggaaa
    14041 aattgctgaa gaacctgaag gaggttttat ggaacgatat gcacaatacg ataaaaaatg
    14101 ataattatgg atttaaaaat aataaaatta ttattttgat acaaatatag agaatgaata
    14161 aaat
//
LOCUS       DASVEV010000034         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_784352, whole genome shotgun sequence.
ACCESSION   DASVEV010000034 DASVEV010000000
VERSION     DASVEV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784352"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..980)
                     /locus_tag="VF222_01760"
     CDS             complement(414..980)
                     /locus_tag="VF222_01760"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7141182.1"
                     /translation="MELPWNDPRSNKFATTIGLITSSGSSGPNIMACEWTHHLSYKPG
                     LIAVSLGPTKSTAGNISHNQEFGINICALDQSILSSIAGGYSGNDYNKISALKELGFE
                     FYNANHIKTLMVKGASLNVECNLYKEITLGDHIMFIGEVIDATHNPKKQPLVYHDGKY
                     WSLQALIKPDTDERKRVREVLEKYKKRK"
     gene            complement(1742..2032)
                     /locus_tag="VF222_01765"
     CDS             complement(1742..2032)
                     /locus_tag="VF222_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141183.1"
                     /translation="MNTAKPDETLLTVEGLRFQGFGPESMTNENDWNINADIQFNEQA
                     IIRLYDENLERKPAYKDQFRSHIVEESYVREGLKTVSFSGNSVEYVLSYEII"
BASE COUNT          832 a          330 c          269 g          914 t
ORIGIN      
        1 gaaaatacaa actaatactg ttatcctatt atttattacc attctataat tatattttaa
       61 tcatatatta atcttatagc gttaataatt taatactaat attatattaa agatatttac
      121 ttaaattata aataaataaa taaatataaa taaatgatat agatgttaat aattttgtgt
      181 tgttaaagtt atagatttag aaatattttt ttatatacat attcaagagt cattcaaatt
      241 aattttatat aatcaatcta gaaattaaaa ttcttgaatt gattacattt aaaggtaaaa
      301 aaagttaagg atagttcaat aggagaatct aattgataac gtaactttta taggtccatt
      361 ataatacttt tggttaaata taactcaatc tatttaaatc ttaaacaaag tatttatttt
      421 cttttcttat atttttctaa gacttctcgt actcttttgc gttcatctgt gtctggttta
      481 attaatgctt gtaaggacca atactttccg tcatgataca ccaatggttg tttttttgga
      541 ttatgtgtag catctattac ttcaccaata aacataatat gatctcctag agtaatttct
      601 ttgtataaat tacactcaac gttcagtgaa gctcccttta ccattaaagt cttaatatga
      661 tttgcattat aaaattcaaa tcctaactct tttaatgcac taattttatt ataatcatta
      721 ccgctgtatc cccccgcaat actggaaagt attgattgat ccaatgcaca aatattaata
      781 ccaaattctt gattatgact tatatttcca gctgtcgact ttgtgggacc tagagaaact
      841 gcaattagac caggtttata tgaaagatga tgtgtccact cgcaagccat tatatttggg
      901 ccagaagatc cactagatgt tattaatcct atagtagttg caaacttatt agaacgaggg
      961 tcattccatg gtaattccat aaattacgat agatagacct ataaaataag gcatgctcta
     1021 taaaatttat caaatcctaa ttatagctaa aaacctcatt atttttatta attgatataa
     1081 aaagggaaag gatttgtatt ctatatttct ccgtatattt gggatatatt ccacaagaaa
     1141 aaacattgca tcaattacat aagataaaat ttacattttg agaatataaa tatcaaatac
     1201 attttataaa tatttattgt ataaaattct attgctaatc aattttggag gaagcaatgc
     1261 cttatacatc taaatcaaga aagacatttg tatgtaccaa ttagtaatat tattaatatt
     1321 ctttatcttc atctttatat aatcataaac aatgaaatta acacataata aatcatcttg
     1381 ttaaatagaa aaagtctact cttgtagtaa aaactttaag gtttaacctt tcatatttta
     1441 ttttcattta taattgtgtc ccttctcggt attttaaatt attttacgtt atatttgtat
     1501 tgagtattag ttatataatt acctgctctc gtcgagttta cttttaatag cactttgaac
     1561 aactttaatt tcttaattta tgtatacatt atttatttag taaatgttct tttgaaataa
     1621 attaaattta atgcaattga aagggtcata cactttataa ataaattata cacagtttag
     1681 acattataga agtttagagg cttatttgct aaactaatca acaaaactca tgtcttcctc
     1741 cttatattat ttcataactt aacacatatt ctacagaatt accactaaaa ctaactgttt
     1801 ttagtccttc acgcacatag ctctcttcaa caatgtgtga tctaaactga tctttataag
     1861 cgggttttcg ttctaaattt tcatcatata gtcttataat agcttgttca ttaaattgaa
     1921 tatctgcatt tatgttccaa tcattttcat tagtcattga ttcgggccca aatccttgga
     1981 acctgagacc ctcaacagta agaagagttt catctggttt agcagtattc ataatcttat
     2041 tgcaatgaag atttactaat ctcaaagttc tcatattgta tttacatatt tttacttata
     2101 aaagattttt tgtggtctta tcaaatacac tttgaactgt catgtacctt aaataaaatt
     2161 ttcggcgccg aaaaaattag ttttatattt attaaaagtg gcataatgtt tttacaatac
     2221 atatatgtat agagataatt atttgcttcg atgctcattg acgtgtactg tttcaacaga
     2281 tttttcagaa gtatattaaa attccagaaa tatccataaa taaagtcgtt attaaagaaa
     2341 ataat
//
LOCUS       DASVEV010000035         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_784426, whole genome shotgun sequence.
ACCESSION   DASVEV010000035 DASVEV010000000
VERSION     DASVEV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784426"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF222_01770"
     CDS             complement(<1..753)
                     /locus_tag="VF222_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141184.1"
                     /translation="MAILFVTVSLLILPDLTHTPVSGQADKSFKGTIAPFVPPSSSER
                     LGSLPGNNFEFDDEEVEGDQEEEEEEIDRDELPVLTKQERDEFLSDNIDAIEADRLTV
                     LSQEETIDEDIDPISSIVEEIVKNETIIGDEEFVPSPESSIIKFKDENLEQILDAQEV
                     APEPDNQTISTTEEEEDLPLNVTEDDFEIEQADEISASESPDVAVQSFINETANEEAT
                     APTDNETAITTEDTIEEEATAPTDNETAITTED"
     gene            complement(1071..1781)
                     /locus_tag="VF222_01775"
     CDS             complement(1071..1781)
                     /locus_tag="VF222_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141185.1"
                     /translation="MKSNLVFVLIAVIFTLLLTIIIFSYNDIDLAFADVTVLEALDYE
                     YVIIGTDEKDRLAGSNSEDIILGLQGNDRIKGKGSSDYIDGGEGKDRIEGNNGNDEIF
                     GNAGSDLIEGNGGGDYIDGHTADDIIQGNSGNDNILGYSGDDVIQGNKGLDNIYGELG
                     DDRIEGNSGNDWLDGGSGRDLLIGGSGDDVLWGKSGKDILIGGPGADEFHCGKGFDII
                     LDYDRSEGDTKSSNCEVF"
     gene            complement(2122..2583)
                     /locus_tag="VF222_01780"
     CDS             complement(2122..2583)
                     /locus_tag="VF222_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7141186.1"
                     /translation="MISKILLPHDGTKISDKTLEKSKEFVKAFNAELYILHVIEHSPL
                     PPSLILDHDRQWIEESRKNIAKKLKEGWIKMAKEKINPVLEKENIKFNITVSTDVQPI
                     SEQILKFASENKIDLIIIGSQRLSEISKIKALGSVSRKVSENVDCPIMIIH"
     gene            3198..4064
                     /locus_tag="VF222_01785"
     CDS             3198..4064
                     /locus_tag="VF222_01785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7141187.1"
                     /translation="MIILLSSTPVLISYSLGEPIKAAIITFDDGMLSQYTYAKPILDK
                     YNSKATFYIICNSVDKENRMNWDNIQILEEEGNEIGSHSMNHERLGNLSDNKMKYEII
                     ESKKCLENKGFNVTSFSFPYNDGDNNKKILKIIADNYNIARTASEPLMLFQCGDETSF
                     VEEEDKYENQCHYSTENNENELQSDFKRYTITGLSHEAYKKSGGYSDDEMYEQFIDIV
                     QSQTKYNTENSIGAIPILIYHMVDYSNDDYSINPDLFEKEMKYLFENGYKIFQMRDIV
                     YDNEEELHIKGY"
BASE COUNT         1429 a          658 c          542 g         1499 t
ORIGIN      
        1 atcctctgtt gttattgcag tttcattgtc agtaggagct gtggcttctt cttcaatagt
       61 atcctctgtt gttattgcag tttcattgtc agtaggagct gtggcttctt catttgcagt
      121 ctcattgata aatgattgaa ccgcaacatc aggagattca gatgctgaaa tttcatctgc
      181 ttgttctatt tcaaaatcat cttcagtgac atttaatgga aggtcctctt cttcttctgt
      241 agtgctaatt gtttggttat caggttctgg tgctacttcc tgagcatcca gtatttgctc
      301 taagttttca tctttaaatt ttattataga tgattctggt gaaggaacaa attcctcatc
      361 accaatgata gtttcatttt ttacaatttc ttcaactata ctagaaattg gatcaatatc
      421 ttcatcgatt gtttcttctt gacttaatac agttaatcga tcggcttcaa tagcatcgat
      481 attatcactt aagaattcat cacgttcttg cttggtcagt actggaagtt catccctatc
      541 aatttcttct tcttcttctt cttggtcacc ttcaacttcc tcatcatcga attcaaaatt
      601 atttccaggt agtgaaccta atctttctga ggaagatgga ggtacaaacg gagctatagt
      661 tcctttgaaa gatttatccg cttgacccga aacaggtgta tgggttaaat caggtaagat
      721 cagaagagat acggttacaa atagtatagc caaaacaaat aacttaaatt tcatcatttt
      781 ttaatatgaa atgaaagcat atatctttaa tataaacaat acttgtttgt tgaaagtagt
      841 ttttttattc acttccttta gaattttata agtaatataa attgggtaag atgtcttgtc
      901 aataatatag gcggatctag attataagat ctccaaatat cattacaatt tgaacctcaa
      961 ctatcaatta taagtatagt tttgattatc ataaacataa taatacttct catgctaaca
     1021 acataaacga tagcgataat aactagatta aaaaaattta ttattggaaa ttaaaagact
     1081 tcacaattgc ttgatttagt atccccttca gatctatcat agtctagtat aatgtcaaat
     1141 cctttgccac aatgaaattc gtcagcacct ggtcctccta ttaatatatc ctttccagat
     1201 tttccccata aaacatcatc acccgagcca ccgattaaaa gatcacgtcc agaacctcca
     1261 tccaaccaat cattgccaga gtttccctca attctatcat caccaagttc tccatatata
     1321 ttgtcaagac ctttatttcc ctgaattaca tcatcaccgc tatatccaag aatattatca
     1381 ttgccagagt ttccttggat tatgtcatct gccgtatgac catctatata gtcacctcct
     1441 ccatttccct caattaggtc acttccagca ttaccaaaaa tttcatcatt tccattattt
     1501 ccttcaattc tatctttacc ctcacctcca tcaatataat cagaagaacc ttttcccttt
     1561 attcgatcat taccttgtaa gccaagaatt atatcttcac tatttgaacc agctaatcga
     1621 tccttctcat ctgtacctat tattacatat tcataatcta atgcttctaa taccgttaca
     1681 tctgcaaatg ctaaatcaat atcattgtaa ctaaaaataa ttattgttaa aagtagagtg
     1741 aaaattactg ctattaaaac aaaaactaaa ttacttttca tagaactact ataaagaata
     1801 atttccaaaa tataaatata cttttatttt tatgataatg aattacttaa tggtctttga
     1861 aaattacgtt ataacacaca tttattattt ctttttaaag atcttcatgt aaatacttca
     1921 ttctaattct tcagaatttc tagaactgat atggattact gccaaaaatg tgtgctgaag
     1981 tctgtagaag atgtgcacaa gaatgtaaaa gaatggttgg tactgtataa tcgaaggaat
     2041 cagtcgaatt ttttatttct gtaataaaat acatcttaat ctttttttat caacttctct
     2101 aattgtaatt acatatatta tttaatgaat tatcattata ggacaatcga cgttttctga
     2161 aacttttctt gaaacactac ctaatgcttt aatttttgaa atttcactta atcgctgact
     2221 accaataatg ataagatcta ttttgttttc tgatgcaaat ttaagaattt gttcactaat
     2281 aggttgtacg tctgttgata cagttatatt aaattttata ttttcttttt ctagtacagg
     2341 atttattttt tcttttgcca tttttatcca cccttccttt aatttctttg caatattttt
     2401 tcttgattct tctatccatt gtctatcatg gtctaaaatt agtgatggtg gaagaggact
     2461 atgttctatt acatgcaata tataaagttc agcattaaat gctttgacaa attctttaga
     2521 tttctcaagt gtcttatctg agatttttgt accgtcatgg ggaagtaaaa ttttacttat
     2581 caacagagat tctctgctcc tacacttttt tcatttattt tccaacagca ttcgtgattt
     2641 ttgttcatct attaacatat caataaactc atagataaaa atagggcttt acaaatggaa
     2701 aatattagca tataacattc tttaacaatt tattattgaa aggaaataga atgaaaatga
     2761 ttgaatagaa gacacggaat agaaactaac ttaatttttt atctaattta aacacctgtt
     2821 aaatatcgag tagagccctt ctaaattatt tcagaaatta ttgaataaaa ataataagca
     2881 aatttatatt ttttatttta atgtaagaag aataaaatta gatcacatcc aaaattttat
     2941 ctgaatgaat aaataaataa ataaactgtt tggggaaata ctatagctat agatattatt
     3001 aggagagctg attttctctt ataaatttaa gaaagtaaag gaaattataa acatacgaaa
     3061 atgactttat acagtaagta atagcataac attaaatcat gtcacaagtt tactaatcaa
     3121 ttaaagtaat aatatctata tatcttgcac ttttttgaat tcttactatg aattggttaa
     3181 caatattcct atttttcatg ataatattat tatcttctac tcccgttctg atttcttatt
     3241 ccttgggtga accaataaaa gcagcaatta taacatttga tgatggaatg ttaagccagt
     3301 atacttatgc aaaaccaatt ttagataaat acaattctaa ggcaactttc tatataattt
     3361 gtaattctgt agataaagaa aatagaatga attgggataa tatccagata cttgaagaag
     3421 aaggaaacga gattggttct cattcaatga atcacgaacg acttggtaac ttatccgata
     3481 ataaaatgaa atatgaaatt atcgaatcca aaaaatgttt agaaaataag ggatttaatg
     3541 taaccagttt ctcatttcca tataatgatg gcgataataa caaaaaaata ctaaaaatta
     3601 ttgctgacaa ttacaatatt gctcgaacgg cttccgagcc tcttatgtta tttcaatgtg
     3661 gagatgagac aagttttgta gaagaagaag ataaatacga aaatcaatgc cattattcta
     3721 ctgaaaataa cgaaaatgaa ttacaatcag atttcaaaag gtatacaata actggactaa
     3781 gtcatgaagc atataaaaaa tctgggggat attctgatga tgaaatgtat gaacaattta
     3841 tagatatagt tcaaagccaa accaaataca acactgaaaa ttcaattgga gcaattccaa
     3901 ttcttattta tcacatggtc gattactcta atgatgatta cagtataaat ccagatttat
     3961 ttgaaaaaga aatgaaatat ttgtttgaaa atggatacaa aatttttcag atgagggata
     4021 ttgtatatga taatgaggaa gaattacata tcaaaggata ttaagttcaa ttcttttcaa
     4081 gtggtaataa ctatcaatta tttagtatta tagctatatt ttaataat
//
LOCUS       DASVEV010000036         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_785185, whole genome shotgun sequence.
ACCESSION   DASVEV010000036 DASVEV010000000
VERSION     DASVEV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785185"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1002
                     /locus_tag="VF222_01790"
     CDS             64..1002
                     /locus_tag="VF222_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7141188.1"
                     /translation="MYYLFIIIVTILSYNIMYVYPVIEESPDISDKSSLQKLNVVASF
                     YPWYEFTKKIGGEKIQLSTAIPFGVEPHEWEPSPQKISELNKADIIIYNSKGFDSWLE
                     QFPQAKLIDVSKGLPLIETNLPEEYSSNDNSQKYDPHIWLDPILVKNISQTINNQLIK
                     LDPINIDYYRKNTNNFIKELDLLDKQIRKSLINCKIHDFITFHESFHYFANRYGLTQH
                     AVHQSLSSEGEILPQQIINIISLTKSLGIDTIYSEELKDQRLSKTFASEIPNGKVLLL
                     SPIEGLDKKELEKNIGYIDKMKNNTENLTIGLKCNL"
     gene            1568..>2004
                     /locus_tag="VF222_01795"
     CDS             1568..>2004
                     /locus_tag="VF222_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141189.1"
                     /translation="MVSRSVYFYGLLIAGTVFIAIGILSIYYSNTSVKVDVDSIIKPD
                     SVDILSPNMDRGNTAFISVSGSLFNMSITYPNKTDVTLTESSSDYSFNLTANQGGEYF
                     IEINNLGNSDILVDGYAFTKGSNLSLIGQIMLIITGVVIAVLG"
BASE COUNT          748 a          276 c          269 g          711 t
ORIGIN      
        1 cttatgtagt atacctaata gtgaaaaatt aaaatatata actgtaaatt tatctttgat
       61 actatgtact atttatttat aattatcgtt actatacttt cttacaatat tatgtatgtc
      121 tatccagtga ttgaagaatc gcctgatata tcagataaat caagtttaca gaaactgaat
      181 gtcgtagcat cattttatcc atggtacgaa tttacaaaaa agataggagg agaaaaaatt
      241 caactctcta ctgccatacc atttggagta gaacctcacg agtgggaacc ctcccctcaa
      301 aagatttcag aattaaataa ggctgacatt atcatatata atagtaaagg attcgactca
      361 tggcttgaac agtttcctca agcaaaattg atagatgtaa gcaaaggatt gccacttatt
      421 gagacgaatt tgcctgaaga atattcttcc aacgataata gccaaaaata tgatcctcat
      481 atttggttag atccaatttt agtaaagaat atttctcaaa caatcaataa tcaattaatc
      541 aaattagatc caattaatat cgattattat agaaaaaata ctaataattt tattaaagaa
      601 ttagatttac ttgataaaca aatacgaaaa tcgttaataa attgtaaaat acatgacttt
      661 attacttttc atgaatcttt ccattatttt gctaataggt atggcttgac gcaacacgct
      721 gtacaccaaa gcttatcttc tgaaggtgaa attcttccac aacaaatcat aaacattatc
      781 tctctcacta aaagccttgg tattgatact atttattctg aagaattaaa ggaccaaaga
      841 ctatcgaaaa cttttgcatc cgaaatacca aatggcaaag ttctattatt aagtcccatt
      901 gaaggtttag ataaaaaaga actggaaaaa aacatagggt atatagataa aatgaaaaat
      961 aatactgaaa atctgacaat aggactaaaa tgtaatctat aattaaatat tatctaattt
     1021 ctaattagaa ttaaatttta tccaatctaa cttctaattt catataattt gttctgttat
     1081 tgatatatat agatagtata catctatata catcttattc ttgatgtata ttttctcatt
     1141 tattaaaaat aagaataatg tattggaaaa ataagtttat gttagattga ataagtcata
     1201 accttaaaga attcttgatg tagttaatta atgtaacctt ataattcaag tcagtgagga
     1261 atcttgtgac gtgtgaaaaa gatattttaa atatctcaca ggaatctgct aaaattcatt
     1321 tttattccta ctatactaat ttaaaggatg gagtaaacac tctcaaatct ttgaaatatc
     1381 tttattacat gcatgactaa ataaaaaatc ttagattaat atatttaaaa ttgcttgcag
     1441 ttctatattt ttgtatctgt ctttaaatac ctataaaata gatttaaagt gtctcttaca
     1501 gatgacaaat atatacaaat atcaaaaata tgaaatctat taatatcttt gctaaattgt
     1561 tatgcctatg gtatcaagat ctgtttattt ctatggttta ttaatagcag gaacagtgtt
     1621 tattgctatt gggattttat ccatttatta ttcaaatact tcagtaaaag tagatgtcga
     1681 tagtattatc aaaccagatt ctgtagacat tctttctcct aatatggata ggggaaatac
     1741 tgcatttata tccgtttcag gatccctttt taacatgtcc ataacatatc ctaataaaac
     1801 tgatgttact ttgacagaat cttcgtcgga ttattctttc aatttaacag ctaatcaagg
     1861 tggagagtat tttatagaaa ttaacaattt aggaaactct gacatattag ttgatggtta
     1921 tgcgtttact aaaggaagta atttatcgtt gattggccaa ataatgttaa taattactgg
     1981 agtagtcata gctgttctag gtat
//
LOCUS       DASVEV010000037        20927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1173958, whole genome shotgun sequence.
ACCESSION   DASVEV010000037 DASVEV010000000
VERSION     DASVEV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 20927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20927
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173958"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF222_01800"
     CDS             <1..826
                     /locus_tag="VF222_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7141190.1"
                     /translation="PERFFNIGIAEANLVSIAAGFAIAGKTAFASTYAAFLPGRCLDQ
                     IRNAICYPSLDVKLVVSHAGLTVGPDGASHQQIEDLSIMRSIPNMKVLAPADSVSVFK
                     IIKNIAYIPGPFYVRLARPSSEIIYDDNESEFQIGKGNILQDGSDITIIGCGLMVYKA
                     LKTAKILKEKFHISARIIDSYSIKPLDNDLVIKAGQETGAIVTVEEHNIIGGLGSAVS
                     EVCSESYPCYVKRVGVRDTFGESARDSELDMLLDCYGLTEKEIERVVLSTIDKLKK"
     gene            823..1491
                     /gene="fsa"
                     /locus_tag="VF222_01805"
     CDS             823..1491
                     /gene="fsa"
                     /locus_tag="VF222_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7141191.1"
                     /translation="MKIFLDTANIVSIRHYSEMGMVDGITTNPTLLSKEGGDPEEIIR
                     EIVQMVQGPVSLEVISTEINQIMEEAYILQRYGSNVVIKVPMIPDGLKAVKKLKEEGI
                     DTNVTLVFSANQALLAAKAGASYVSPFIGRLDDIGNEGMSLIRDINQIFKNYQYNTKI
                     LVASIRHPIHVIESAKAGADVVTLPPDILGKMFLHPLTDNGLKTFISDWENLKKTNPS
                     IKIL"
     gene            complement(1515..3095)
                     /locus_tag="VF222_01810"
     CDS             complement(1515..3095)
                     /locus_tag="VF222_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7141192.1"
                     /translation="MDNSKKYLRNDILLDIATFLARRWSNSAKVIVTISKDKSPQTDI
                     EKQQIILPTLNYFHGEEFQRYRQWRISLWYEAMRIRHTSKVKVSRQDHAYGFILNSIE
                     TKRIEILGLHDWQGMTNEIIFYEGMSWLSKPLLNTIYGRHKIIEAFSQYFLTGYIKGE
                     LFGNEFDKIRQATDFANECIKEAIDKRLGTDWIYDKVREIIKILQIDPLLSIPLTTPR
                     SSIGLAMNKLDLFKQLESIIKSKLVNKEFDKTIKEIIEGKDVVKEYETLVKESKKSET
                     KGYDSLENFDISVPNRMDINESEIYDIELIQKIKSAFRDWKTGWIELHQEKGDELDTE
                     AYIDSQTKPFLSDFKISIRTKVVILLDHSSSIEEIELKYKQATIALCEALSYLGIDFA
                     VYAFSTEAKKVKCWLIKPPKIPWSAINARRLAQIRASGGTPLAEIYTILLPFIKSFKP
                     EIMITLTDGEPSNYDAVKEMVLTYRNFSIHMAALGLGKDLNDLINIGHNLKYLNYNKT
                     IASRVEDIPKKVINLLKV"
     gene            complement(3104..3865)
                     /locus_tag="VF222_01815"
     CDS             complement(3104..3865)
                     /locus_tag="VF222_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7141193.1"
                     /translation="MDILKKAYKINLFAIIIGPKGTGKTSLARKFASELNKKIFSVNF
                     SLRTRESHLVGTKTIENGEINFVEGILVKSMKEGGILYLDELNAAEPDMLLRLDEALD
                     DRRQIILKEAEGQLIKASEGWFVVATINPLSHVGTKELPPQLLSRFPIRLSLDYPPEE
                     TELEIIKQHTIVNNDNLINIQRAIKLARALREAAGVEELFYGPSLRETIAFTKLLNIG
                     TDPKLAAEIVFANAYNQWGEVEYRKVMDMVLSIFG"
     gene            complement(3969..5369)
                     /locus_tag="VF222_01820"
     CDS             complement(3969..5369)
                     /locus_tag="VF222_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7141194.1"
                     /translation="MKILNKSIDFVYFPFTKESDQTPNKKFEDQGEQNISNDILNDLL
                     KSLGKEILKEKTPSLQELEQTLQEIIKKKENNKNKLNKESDINDNNDSNTPTDSNVKS
                     NLPIVTHLIRKGYIKNSEKWLSSKGFVSIGEKILQDILKELKKGDLGIHETKNFGYGS
                     LLLDTTKKYEIGDDIRLINIPNSLLNSIQRLARKNGRVEIPIKFEVEDFEEYETIQDV
                     RVSIVYCIDLSSTMRYATLYGDLSRIEAAKRALWSLYILHKKYFPSDIINMVGFGALA
                     SKINPQDIPYLKTFEPGVDFLHYTNYQAALKLAKKILQKNPVENKRIVLITDGHPSAC
                     FIDNEIEQQKILSQRPYSHFYIPDKNTINSIKKSQEMDLDINSGELVYLCYRYRQVDQ
                     FIGERTIIEAKKCYKKGIQIDTIMISEEDSMLSYVNEMEKYVKGRSYYINPDSIDKML
                     LTDYINNKKKIIHSKR"
     gene            complement(5353..7014)
                     /locus_tag="VF222_01825"
     CDS             complement(5353..7014)
                     /locus_tag="VF222_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7141195.1"
                     /translation="MNFKDIEKMVLDSYKSDPTYNEIFPGVPQNYKEFKTLKDLLEIN
                     YKHAKVDDQIRGNLITLLKKGINPYRGIIGYDDDVIPSINRGILAGHDLLLVGQIGQA
                     KTKLSEAISKNLLSPIPIIRKSLTNDIPTTIIAEQLISLLEDKEVIHYSPRFTVSKET
                     EDNIRNNGLDTRVDWVDGSERYRYILATPDISVKDLVGQIDAIKIAKKGVEIYNIESY
                     SPGQLLQARHGILCIDELPVLDPRKQVSLLSVLQEGKFTTGSYPVYFEPDLKIIATSN
                     PIDYTHSGKIIEPLFDRLRSHIETRYPVKIIDEMLIIIQEIKLSDPKNIILPIFVLKT
                     IAKITQLARTNSDINQDKGVSVRMSVHSAEAIISEAERVRSLTYNVKAVPRFSDINSI
                     HQTSKFELSELDDNKQNRLEILNKLISEAINAIAKEYIQNIKKEEIVLLKNDFGKNKK
                     FIVSQNLLGEESSNSPDNYENQLKSFPILLKIVDRIIEIDEKEKNVFIKNIEYNRIDI
                     KGLSFSNKQNKEYRASIVELILEGLRNLDNPIIDRREKGIYENTQ"
     gene            7393..7875
                     /locus_tag="VF222_01830"
     CDS             7393..7875
                     /locus_tag="VF222_01830"
                     /inference="COORDINATES: protein motif:HMM:NF003140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7141196.1"
                     /translation="MSTADYKHIVRIMGKDVEGDKKITVALSKVKGIGYNFAQVLLQK
                     LNINPYIRVGLLTDKELNDIEDALKDPTKIGIPYFYFNRRKDMDTGYNIHLLTSDLDF
                     IVSNDIDREKSIMSWRGYRHMFGLRVRGQRTRTTGRRASAVGVRKTAQAPPKTQKSGE
                     "
     gene            7875..8384
                     /locus_tag="VF222_01835"
     CDS             7875..8384
                     /locus_tag="VF222_01835"
                     /inference="COORDINATES: protein motif:HMM:NF003139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7141197.1"
                     /translation="MGDPKKSRKTFRRPRQIWTTDQLSSELYLIGSYGLRSKSELWKA
                     QTKVADIRNQARELLALTIEARQTQETKLLNYLQRLGLVKDSATLDDILNLKLEDILE
                     RRLQTIIMKKDNAKSPHQSRQLVVHGHVSIGERIINIPGYIVKKEEESKIALRGQFMN
                     PSQNIPTSQ"
     gene            complement(8385..9341)
                     /locus_tag="VF222_01840"
     CDS             complement(8385..9341)
                     /locus_tag="VF222_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141198.1"
                     /translation="MRKKTIISDHNGNTISKKILIFDNITDMKTLSNNIAWKIIEILS
                     SRAMYPAQVAKELKLYDQTVYYYIRKLVRIGAIEQVGTSLIRGGTARLYSTSSSSFGL
                     ELEGHGEKLESSHYTKNDTRKNIPYILKEFYENNSFSGLIVVGAPDPHGPYKSSSRDG
                     HYAVHLSFYLGTLCNSSTSGFIVKLDVDAKAEKDIDNRNLILIGGPGTNIVTSEFNRY
                     LQIKFNADNYWSGLTDQSGRIFNMDNHGLIAKINNPYNKDKKILILAGVRSIGTKASV
                     IALTNYGNKICDNSLGDDQFALVVQGFDMNADGKIDHVDIVS"
     gene            9509..11275
                     /gene="glmS"
                     /locus_tag="VF222_01845"
     CDS             9509..11275
                     /gene="glmS"
                     /locus_tag="VF222_01845"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7141199.1"
                     /translation="MCSIIGYIGKCMAASVIVESLKKMEYRGYDSVGIATLERDRILL
                     RKDVGKVLDVDNSLKLYNMPGQIGIGHTRWATHGGITKLNAHPHTDCTNNVVVVHNGI
                     IENYQQLKDNLIKQGHIFQSQTDSEVIAHLIESYLKKFDDVKKVIIETCKQLHGSYAF
                     VAIFKSGLIAGTRLDEPLIIGMGNDGNFISSDVLGFLEYTDKAIFLDNRDIVILDHQS
                     INLYDFNGNNVFRPITQVAWELGDIDKGKYAHYTIKEIHQQINSIPVAFQIDRISLKK
                     ATDLISSSKNIFITGSGTSYHSALLAKYLFYKFAKIRTEIIMSSEFEYMSAPIDENSV
                     LIAISQSGETADVLNSVKIAKEYKAKILSIVNIPTSSLSRLSDHYVSINCGPEMGVAA
                     TKSFTAQLATIYSIIDSLGVNNRSLDMSTDVFNNALNEILMDDSYLEKIAEDIKSVQD
                     IYILGKSFHYPIALEGSLKIKELAYIHAEGIAAGEIKHGPLAMIELNTIVIVLNPEDE
                     TYYDTLHSIHEMKSRGAKIIGISNKNNDMYDYFIKLPLVNENFYPIIEIIPIQILSYY
                     LSLKKDANPDYPRNLAKSVTVK"
     gene            complement(11308..12432)
                     /locus_tag="VF222_01850"
     CDS             complement(11308..12432)
                     /locus_tag="VF222_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HEX7141200.1"
                     /translation="MIEKYKDITVVNGKYSIDILESSNPIFVCVISYTETSEIPGITV
                     AGANNDLIKYTSSADAEFLYYGKCKCIDGVPATPDGKPTPAIITKTMLNIGNIPFIVV
                     DSGAKIKPDIPLFSFNLRSGKNIMENIAMESEEVEKAYRYGLLLGKQLSKSNDLVILG
                     ESIPGGTTTALAVMLSLGIDAKNKMSSSMPENPHNLKNMIVIKSMKKNNITFGSLKDK
                     PFEAISLLGDPMIPSVTGIAQGVIDNGGRVMLAGGTQMCTILSILKSLNVKTDKVVIG
                     TTKYIFNDHSADFTNLVLSIDNNVGIVISDLHLIESTKLGLKAFANGFVKEGVGAGGV
                     SIATMINANGKLNGEILLKCIEREYEKNIEKKFTLNGINI"
     gene            complement(12437..13063)
                     /locus_tag="VF222_01855"
     CDS             complement(12437..13063)
                     /locus_tag="VF222_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7141201.1"
                     /translation="MTFKIEQISVGKMANFTYLIIDNEEKETAIVDPSWDLEKIFEII
                     NKNEYKVKYVINTHSHFDHILGNDQIVVHTKCKVVQHKNSREKHDISLNDGEEFMIGK
                     TIVQVLFTPGHSKDSICLIVDSKIIITGDTLFVGNCGRIDLPGSDPEEMYNSLFKKIM
                     NLDNNLIVYPGHDYGYKPTSTIEEEKKSNYILVPRSRNEFIRFITQGE"
     gene            complement(13065..13802)
                     /locus_tag="VF222_01860"
     CDS             complement(13065..13802)
                     /locus_tag="VF222_01860"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX7141202.1"
                     /translation="MNHPKVKNEKNNKIIKKMSEIAISSHQMYEIENIGHTKFGMKKV
                     LMMENAGFGVADFIIKRFKNKKVSKLKILAICGSGNNGGDAMVAARHLTCLGINVKVI
                     FLGETSSIKTEEALTNFQIIRKMNNTIELILNSKLDTTNIKKQILNADIIIDGIFGTG
                     IKGDIQDPHLSVIKLVNKSKSYIVSVDIPSGLDPNNGKITADCVRANTTITFHRIKIG
                     LLNNRKYTGNLILKKIGIPIEAERGIV"
     gene            complement(13812..14333)
                     /locus_tag="VF222_01865"
     CDS             complement(13812..14333)
                     /locus_tag="VF222_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141203.1"
                     /translation="MGNIRRSRGYSYEYSIVNRINNRNWIAKRLGGSSVGLPDIIAVN
                     NNKGILLVIEAKSGTGESLYVPSDQIERCFSIVKMFRYYNYKYVILAFKFLRKKRIKV
                     NNELSYEKRKLVEYYKIIEPIWKKGKTPQIKCNYDGKTFLITSNGSLEINFSNYKMHF
                     QKKDIKLNTNTPP"
     gene            14446..15066
                     /locus_tag="VF222_01870"
     CDS             14446..15066
                     /locus_tag="VF222_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7141204.1"
                     /translation="MVTEKIEISLKSINDELHSIENRREQLIKESRDVIILCSQSIIS
                     LHQGHIQESQLKLDKAKDLYSSLKTLAQVDLLRYLSMSEQELVESSLLMSIVKNEPLP
                     GSDEIGVSSQAYLFGILDCIGEIKRMVYDMVRLGRYEQAESLFRIMQEIYRYLYPFSI
                     YDNIVSGIRKKLDICKILIENTRELITEESRRSILIESLKKLDSRL"
     gene            15147..17270
                     /locus_tag="VF222_01875"
     CDS             15147..17270
                     /locus_tag="VF222_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7141205.1"
                     /translation="MRDSEKVQKIINDVTQTGNKVITEEDAKSILSVYEIKVPAYSIV
                     TTESEAVSKAKEIGFPLVAKIVSDQILHKTDVQGVKVGLQNENEVKETFLDMYNRLSK
                     DYKVKGVLLEKMVPKGIELIVGLQNDPQFGPVIMLGLGGIYTELFKDVSFRVLPITKE
                     DVLNMIEDLKAKEILKGFRGAKPVNLEMLCDAILKIGNLGIDMAKYIDSIDFNPIIVY
                     PDNYYVVDAKILLKSIPDENIISQSTPDSSYMNTFFDTNSIALIGASPEEGKIGNSVL
                     KSLSKYDYKGKIYPVNAKGYNEILGLKAYKNLEEISENIDLVIVTVDLKFVPTILETC
                     GMKGIHNMVIISGGGKELGGERAEIEQKVKELSSKLKVRIIGPNCIGMFNGENRLDCA
                     FQGHDRMVRPKKGDVAFLSQSGTIGIAFMETSDTFGMSKMISYGNRSDVDEADMIWYL
                     SDDPNTNVIGLYVEGLGNGRKFMNTARRVIKEKKKPIVVFKNGRTQRGAKQAASHTGS
                     LGGSYSVVKGAFDQAGIISVDSYEELTGSLKALTWQPIPQGNKVALVTNGAGPIIAAI
                     DHFERLGLKIADLSEDSIHKFNEHYPATYVIGNPLDVTGSANADDYKFAIQTFMDDPN
                     VDIIMPWFVFQDDPLEETIVNILGDFQKQKKKPILVGALGGPFTIDISNRLEKKMVPV
                     YHSVLSWAVAAGVLAKWSELKNRKS"
     gene            17321..17671
                     /gene="erpA"
                     /locus_tag="VF222_01880"
     CDS             17321..17671
                     /gene="erpA"
                     /locus_tag="VF222_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX7141206.1"
                     /translation="MESTQQSQIVTVTPKAAEKVSEFMKQEGKDSLFLRVYVTGGGCS
                     GLSYGMGFEENSEEDDLVIEQNGVKIVVDNYSQRYLKGANIDYIDSLMGSGFKINNPN
                     VTKSCSCGHSFSTE"
     gene            complement(17757..19025)
                     /locus_tag="VF222_01885"
     CDS             complement(17757..19025)
                     /locus_tag="VF222_01885"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7141207.1"
                     /translation="MSSSNLNEKINPFEVALKQLQEASKILNLDNGMFEILSNPKRIL
                     TVSIPTRMDDGSIKVFTGFRSQHNDARGPHKGGIRYHPDVTLDEVKALSMWMTWKCAI
                     ANIPYGGGKGGIICNPKQMSENELERMTRRFAYLIADIIGPYRDIPAPDVYTGGKEMA
                     WIMDTYSALKGNYVQPEVITGKPIAIGGSLGRTEATGRGLAYTVREAAKKINIDMKQA
                     TFVVQGFGNAGQFSSQLVEEQGAKMIAASDSQGAIIKTDGISVDALMKFKRETGSIVG
                     FGDAKSITNDELLEIECTILIPAALENQITKKNADKIKTQIIAEAANGPTTPEADEIL
                     FKKDILVIPDVLANGGGVTVSYFEWLQNLRREYWTEEEVNNRLDINITKSFYDVYDIH
                     TKYNVDMRKAATLLAVQRVVEAINIRGLWP"
     gene            complement(19074..19433)
                     /locus_tag="VF222_01890"
     CDS             complement(19074..19433)
                     /locus_tag="VF222_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7141208.1"
                     /translation="MGIGGSEWVIIGLVTIFLLFGSRKLPDMTRHIGKAIGEYNNTRQ
                     TLLKQVNKVKASNSTFDDELNNEKNLYFGKIIHGPVSTEREKLEKIAASLNIDYLDIS
                     DEQLRLIILKKMKNDNF"
     gene            19622..20896
                     /gene="dnaG"
                     /locus_tag="VF222_01895"
     CDS             19622..20896
                     /gene="dnaG"
                     /locus_tag="VF222_01895"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF003108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HEX7141209.1"
                     /translation="MELPNTGIVKYHVKLSFDVDGLVEKADIIGAIFGQTEGLLGPEM
                     NLNELQKVSKVGRIEVNVETKSNIAKGDALIPMSTDISTAALIAAAIESIDKVGPFQA
                     RFELSGIDDIRAIKKKVIVDRAKKIVQEWATKTISEGEEMLKDVYDASKPGKLTTFGK
                     GQLACGTGVFNADWVILVEGRADVINLLRAGFDNSIAIEGAKIDESITHLTEGKKVIA
                     FLDGDRAGDLILKELHGLVKIDKVFRAPAGREVEECTPLEITEILNNAVDDPKEDSFS
                     TIKKQQSVLQNHKKYNKNDREKTSFQQTQPQFNHLETYPITKEDNGLQTNNESKSVIR
                     NIYSQINETLEAIILDGSMNTIMKVPVSEIVKKLSDSSGGKILVLDGIVTQRLIDASD
                     KAGIEQIIGHRLADLKRSSSIKVGTFNEMGLQ"
BASE COUNT         6921 a         3226 c         3173 g         7607 t
ORIGIN      
        1 tcctgaacgt ttttttaata taggaatagc tgaggcaaat cttgtttcta ttgcagcagg
       61 tttcgcaatt gctggaaaaa cagcatttgc aagcacatat gctgcatttt tacctggacg
      121 ttgtttggat cagattcgta acgcaatatg ttatcctagc ctagatgtta aattggttgt
      181 atctcatgct ggtttaacag tcggcccaga tggagcctca catcagcaaa ttgaagattt
      241 atctattatg agatcaattc ctaacatgaa agtccttgca cctgctgatt ctgtatctgt
      301 tttcaaaata attaaaaata ttgcatatat tccaggtcca ttttatgtaa gacttgctcg
      361 tccctcgtca gagataattt atgatgacaa tgagagtgaa ttccaaatag gtaaaggaaa
      421 tatattgcaa gatggttcag atataactat tattggttgc ggattgatgg tttataaagc
      481 ccttaaaaca gctaaaattt tgaaagaaaa attccacata tccgctcgta taattgattc
      541 ttattcaata aagccattag ataatgattt agtgataaaa gcaggacaag aaactggtgc
      601 gattgttact gttgaagaac ataatataat aggaggttta ggtagtgcag tatcagaggt
      661 atgttctgaa tcttatcctt gttatgtaaa acgagttgga gttagagata catttggaga
      721 atctgcaaga gattctgaat tagatatgtt gcttgattgt tatggtttaa ccgaaaaaga
      781 aatagaacgg gttgtgctat ctacaattga taaattaaaa aaatgaaaat atttcttgat
      841 actgctaata ttgtttccat tcgtcattat tccgaaatgg gaatggttga tggaataacg
      901 actaatccta cacttctttc aaaagaaggt ggagatccgg aagaaatcat tagagaaatc
      961 gtacagatgg tccaaggacc agttagccta gaggttatat ctactgaaat caatcaaata
     1021 atggaagaag cttatattct ccagcgatat ggttcaaatg ttgtaattaa ggttccaatg
     1081 attcctgatg gattaaaagc agtaaaaaaa ttaaaagaag aaggaataga tacaaatgtt
     1141 actctagttt tttctgctaa tcaagcttta ttagctgcaa aggcaggagc atcatatgta
     1201 agtcctttta ttgggagact tgatgatata ggtaatgagg ggatgagtct tatcagagac
     1261 ataaaccaaa tttttaaaaa ttatcaatat aatactaaaa ttcttgtggc aagtatacgt
     1321 catccaattc atgtgattga atctgctaaa gctggtgcag atgtagtaac tttaccaccc
     1381 gatattcttg gaaaaatgtt tttacatcct cttacagata atggcttaaa aacattcatt
     1441 tcagattggg aaaatcttaa gaaaacaaat ccttcaatta agattttata actatatctt
     1501 tattttatga gtaattacac tttgagtaag ttaataacct tttttggtat atcttctact
     1561 ctactagcta ttgttttatt gtaattaaga tattttaaat tgtgaccaat attgataaga
     1621 tcatttagat cttttcctaa tccaagggct gccatgtgga tagagaaatt tctataggtt
     1681 aaaaccattt ccttaacggc atcataattt gaaggctctc cgtcagttag tgtaatcatt
     1741 atctcaggtt taaatgattt gataaaaggt aaaagaatag tgtatatttc agctaatggt
     1801 gtacccccac ttgctcttat ttgagctaat cttctggcat ttattgcaga ccatggaatt
     1861 tttggtggtt taattagcca acattttact ttttttgctt cagtactaaa tgcatagaca
     1921 gcaaaatcga ttcctaagta actaagggct tcacataatg ctatggtggc ttgtttatat
     1981 ttgagttcaa tttcttctat actactagag tgatcaagta aaatcactac ttttgttcta
     2041 atggatattt taaagtcgct gagaaaaggt ttagtctgag aatcaatgta tgcctctgtg
     2101 tccaattcat cgcctttttc ttgatgaagt tctatccaac cagttttcca atctctaaat
     2161 gccgatttaa ttttttgaat gagttctatg tcatagattt ctgattcatt gatatccatc
     2221 ctattaggaa cactaatgtc aaaattttct aaactgtcgt atccttttgt ttctgatttc
     2281 ttactttctt taactaaagt ctcatattct tttacgacat cttttccttc aataatttct
     2341 tttattgttt tgtcaaattc tttatttacc agttttgatt ttattatact ctctagttgt
     2401 ttaaataaat ctaatttatt cattgccaat cctatactag atctaggagt agtcaaagga
     2461 attgataata aaggatctat ttgtaatatt tttataattt ctcttacctt gtcatatatc
     2521 cagtctgttc ctaatctttt atcaatagct tctttaatac actcatttgc aaaatctgtt
     2581 gcttgtctta tcttgtcaaa ttcatttcca aatagttctc ctttaatata tccagtaaga
     2641 aagtattgtg aaaaagcttc gattatttta tgtcttccat atattgtatt gaggagaggt
     2701 tttgataacc acgacatacc ctcataaaaa attatttcat ttgtcatacc ttgccagtca
     2761 tgtaaaccta atatttcgat tctttttgtt tctatactgt taagaataaa tccatatgca
     2821 tggtcttgac gtgagacttt tacttttgaa gtatgcctta ttctcattgc ttcataccat
     2881 aaagaaattc tccattgtct atatctttga aattcttcac catgaaaata atttaatgtt
     2941 ggtaaaataa tctgttgttt ttcaatatct gtttgagggg atttatcttt agaaatggta
     3001 acaatgactt ttgcactatt agaccatcta cgtgcaagaa atgtagctat gtctagcaga
     3061 atatcatttc ttaaatattt tttactatta tccaatgtaa ttatcaaccg aatatcgaaa
     3121 gaaccatgtc catgaccttt ctatactcta cttcacccca ttgattatat gcatttgcaa
     3181 aaactatttc agctgcaagc tttgggtcgg taccaatatt tagtaattta gtaaatgcaa
     3241 ttgtttctct taaactaggt ccataaaata gttcttctac tcccgcagct tctcttaatg
     3301 ctcttgctaa ttttatagca cgttgaatat taattaaatt gtcgttattt actatagtat
     3361 gttgtttaat aatttcgagt tcagtttcct ctggtggata atctaaacta agcctaattg
     3421 ggaatctact taaaagttga ggtggtaatt ctttagtgcc aacgtgggat aaaggattaa
     3481 ttgtagcaac tacaaaccat ccttcactag ctttaataag ctgaccttct gcttctttca
     3541 atattatttg tctcctatcg tctagagcct catctaatct taagagcata tctggttctg
     3601 cagcatttaa ttcatccaaa taaagaattc ctccttcttt catggatttt accaaaattc
     3661 cctccacaaa gttgatttca ccattctcta tagttttagt tccgactaaa tgagattctc
     3721 ttgttctaag actaaagttt actgaaaaaa tttttttatt aagttcagag gcaaattttc
     3781 ttgccaatga tgtttttcct gttcccttag gaccaattat aatagcaaaa agatttattt
     3841 tgtaagcttt ttttaatata tccatagatt gattccagtc tagataattg atggtctcca
     3901 ttacattact cattgttaac attacagatt gagtttaatt tataaattaa ctaaataagt
     3961 atcagaaatt atctttttga atgaataatt ttctttttat tatttatata atcagttaat
     4021 aacattttat ctattgaatc tggattaatg taatatgatc ttccctttac atatttttcc
     4081 atttcattta cataactcaa catagagtct tcttcactta tcattatagt gtcaatttga
     4141 attccttttt tatagcattt tttggcttca attatagtac gttcacctat aaattgatca
     4201 acttgtctat atctataaca taaataaact aattcgcctg aatttatatc taaatccatt
     4261 tcctgacttt tcttaataga atttattgta tttttatcag gaatatagaa gtgagaatag
     4321 ggtctttgtg aaagtatttt ttgctgctct atttcattat caataaaaca tgcactaggg
     4381 tgaccatcgg taataagcac tattctttta ttttctacag gatttttttg taaaattttt
     4441 ttggctagtt ttaatgctgc ttgataattt gtataatgaa gaaaatcaac tccaggttca
     4501 aaagttttta aatatgggat atcttgtgga tttattttag aagctaaggc cccaaatcct
     4561 accatgttaa ttatgtctga aggaaaatat tttttatgta ggatatacaa agaccataat
     4621 gctctcttag cagcttctat tctacttaaa tccccgtaca atgttgcata tctcattgtg
     4681 gaactgagat ctatacaata tacaattgaa actctaacat cttgaatagt ttcatattct
     4741 tcaaaatctt caacctcaaa ttttatcggt atttcgactc ttccattttt tcttgcgagc
     4801 ctttgaattg aatttaataa tgaatttgga atattaatca atctgatatc atcaccaatt
     4861 tcgtattttt ttgttgtatc taataaaagg gatccatatc caaagttttt ggtctcatgt
     4921 attcccaaat ctcctttttt tagttctttc aatatgtctt gtaatatttt ttcaccaata
     4981 ctcacaaatc ctttactgct taaccatttt tcggaatttt ttatataacc ttttcttata
     5041 agatgagtaa caataggtag attcgatttt acattgctgt ctgttggtgt atttgaatca
     5101 ttattgtcgt taatatcgct ttctttgtta agtttatttt tgttattttc ttttttttta
     5161 ataatttctt gtaaagtttg ttctaattct tgtaaagatg gtgttttctc tttaagaatt
     5221 tcttttccaa gggattttaa taggtcattt agtatatcat tacttatgtt ttgttctcct
     5281 tgatcttcaa actttttatt cggagtttga tccgattctt tagtaaaagg aaaatataca
     5341 aaatctattg acttattgag tattttcata tattcccttt tctcttctat caataattgg
     5401 attatctaaa ttccttaacc cttcaagtat taattctact attgatgctc tatattcttt
     5461 attttgttta ttagaaaacg ataatccctt aatatcaatt ctgttatatt caatattttt
     5521 aataaagaca tttttttctt tttcgtctat ttctattatc ctatcaacta tttttaaaag
     5581 aatcggaaaa cttttcaatt gattttcgta attatcaggg gaattgctgc tctcctctcc
     5641 taaaagattt tgggatacta taaatttctt attcttacca aaatcatttt ttaagagcac
     5701 aatttcttcc ttctttatgt tttggatata ttctttcgct atagcgttta ttgcttctga
     5761 aatcaattta tttaaaatct ctaatctatt ttgtttatta tcatctaatt cagataattc
     5821 aaatttggat gtttgatgta tactattaat atctgaaaat ctggggactg cctttacatt
     5881 ataagttaaa gatcttactc gttcagcctc actaattatg gcttcagcgg agtgaaccga
     5941 cattctcaca cttacccctt tatcttggtt aatgtctgaa tttgttctag ctaactgtgt
     6001 tatcttagca atggttttca agacgaaaat aggtaaaata atattttttg gatcgcttag
     6061 ttttatttcc tggattatta taagcatttc atcaatgatt tttacaggat aacgtgtctc
     6121 gatatgactt ctaagcctat caaacaaagg ttctattatt tttccagaat gagtataatc
     6181 aatcggattt gaggtagcta ttattttgag atcaggttca aagtaaacag gatatgaacc
     6241 ggtagtaaat ttaccttctt gcaatacgct caataaagag acttgttttc taggatctaa
     6301 tacaggtaat tcatcaatac acaatattcc atgtcttgct tgtaatagct gaccaggtga
     6361 gtatgattct atattgtaaa tctcaactcc tttttttgct atttttattg catcaatttg
     6421 tcctactaaa tctttaaccg aaatatcagg agttgctaaa atgtatctat atctttccga
     6481 tccgtcaacc caatctactc tagtgtctaa tccgttattc ctaatattat cttccgtttc
     6541 ctttgaaaca gtgaacctgg gcgaatagtg tataacttct ttgtcttcta ataaagaaat
     6601 cagctgttcc gctataatcg ttgtgggaat atcatttgtt aaagactttc taattatggg
     6661 tatgggcgat aatagatttt ttgatatagc ttcagataat tttgttttag cttgacctat
     6721 ttgtcctact aataacagat catgaccagc aaggattcct ctgtttatgg atggtattac
     6781 atcatcgtca tatccaatga ttccacggta tggatttata ccttttttca ggagtgttat
     6841 taaattacct ctaatttgat catcaacttt agcatgtttg tagtttattt cgagaagatc
     6901 ttttaatgtc ttaaattctt tataattttg tggaacacca ggaaatattt cattatatgt
     6961 tggatcagat ttgtaagaat ctaataccat tttctcaata tctttaaaat tcaattgatt
     7021 tcttgcttac taaaacaaaa tttaaacatt tgttaaataa atagatagta gtagtcattc
     7081 aaaattattt atactagtaa taaaattctt ggaattttct tgatcattct agtattttta
     7141 aaaaactgta atatgttttc tatattatat gtattcactt ttttcattct gttattgtat
     7201 ttttttagtt ttttttatta attatatgga tagatagcat tttaataaaa aaattttagc
     7261 taagatttta taaaatatct aatatttgag ttgattttat taaatcgacg gtctgtgtga
     7321 ttataaatta aaattacttt aacctaaatc ccagtatgat ttttattgag gtctttactc
     7381 tagttttaag agttgtcaac agcagactat aagcacattg ttagaattat gggtaaagat
     7441 gttgagggag ataaaaaaat aactgtcgct ttgagtaaag ttaaaggtat tggttataat
     7501 ttcgctcaag tattacttca aaaattaaat attaatccat atattagagt cggtcttcta
     7561 actgataaag aattaaatga tatagaggac gctcttaagg atcctactaa aataggtatt
     7621 ccatatttct atttcaacag gagaaaagat atggatacgg gttacaatat tcatttactt
     7681 acatccgatt tggattttat tgtttcaaat gatatagata gagagaaaag tattatgagt
     7741 tggagaggat atagacatat gtttggatta cgtgtaagag gtcaacgtac acgtacgact
     7801 ggtagaagag ctagtgcagt aggagtacgt aaaacagccc aagctccacc aaaaacacaa
     7861 aagagtggtg aataatggga gaccctaaga aatcaagaaa aacttttcgt agacctcgtc
     7921 agatatggac taccgatcag ttaagttcag agttgtatct tataggatct tatggtctta
     7981 gaagcaaaag tgaattatgg aaggcacaaa ctaaggtcgc agatataaga aatcaagcgc
     8041 gtgaattatt agctttaaca attgaggcaa gacagacaca agaaaccaaa ctattaaact
     8101 atttacaaag acttggatta gtaaaagatt ctgcaactct agatgatatt cttaatttaa
     8161 aactagaaga tattttagag cgaagattgc aaaccatcat aatgaaaaag gataatgcca
     8221 aatctccaca tcaatcaagg cagttagtag tgcacggtca tgtatcaatt ggcgaaagga
     8281 ttattaatat tccaggttac atagtcaaaa aagaggaaga atcaaaaatt gccttaagag
     8341 gtcaatttat gaatccatct caaaatatac cgaccagtca ataattaaga aacaatatca
     8401 acatgatcta tttttccatc tgcattcatg tcgaaccctt gaacaactaa tgcgaattga
     8461 tcatcaccta aagaattatc acatatttta tttccataat tggttaatgc aatgaccgat
     8521 gcttttgttc ctatacttct tacacctgct aaaataagga tttttttatc tttgttatag
     8581 ggattattta ttttagcaat cagtccatga ttgtccatat taaatattct tcctgattgg
     8641 tcagttaatc cagaccaata attatctgca ttaaatttaa tctgaagata tctattgaat
     8701 tctgaagtaa caatatttgt gcctggccca ccaatcaata ttaaatttct attatcaata
     8761 tctttttctg ctttcgcatc tacatctaat ttaactatga agccagaagt agatgaatta
     8821 cataaagttc ccaaataaaa actcaaatga acagcataat gtccgtccct agaggaggat
     8881 ttgtaaggac cgtgaggatc aggtgctcct acaacgatta acccagaaaa agagttattt
     8941 tcataaaact cttttaatat ataaggaata ttttttctcg tgtcattttt tgtgtaatga
     9001 gaagattcaa gtttctctcc gtgtccttca agttctaaac caaatgaaga actagaagtt
     9061 gagtaaagcc tagctgttcc tcctctaatc aaacttgttc ctacctgttc tattgctcct
     9121 attctaacca attttctaat ataataataa actgtttgat cataaagctt taattccttt
     9181 gctacttgtg ctggatacat agctctggat gataatatct ctattatttt ccatgcaata
     9241 ttatttgata atgttttcat atcagttata ttatcaaata ttaaaatttt tttacttatg
     9301 gtatttccat tatggtcaga tattatagtc ttttttctca taatgtcaaa tcgactattt
     9361 tctataaaac gttttatacc ggtaaacaaa tatcataatg acttttctaa tattgatttg
     9421 aaattaagat cacaattatg aatatcttta tagtggtata gtacaagtgt taatatacta
     9481 tttaaactaa tttcatatga cataatttat gtgttctatt atagggtata ttggtaaatg
     9541 tatggcagca tcggttatag ttgaaagttt aaagaaaatg gaatataggg gatatgatag
     9601 tgttggaatt gctacccttg aaagagacag aatcttactt cgtaaagacg taggaaaggt
     9661 tcttgatgtt gataactctt taaaattata caatatgcct ggacaaatag gaataggaca
     9721 cacacggtgg gctacccacg gaggtataac taaattaaat gcacatcctc atacggattg
     9781 tacaaataat gtagttgtag ttcataatgg aataatcgag aattatcaac aactaaagga
     9841 taatcttata aagcaaggac atatttttca aagtcaaact gatagtgaag ttattgcaca
     9901 cttaatagag agctatctta aaaagtttga tgatgtcaaa aaggttatta ttgaaacatg
     9961 taaacaatta cacggttctt atgcatttgt ggcaattttc aaatctggat tgatcgctgg
    10021 tactagatta gatgaaccat taattattgg aatgggtaat gatggcaatt tcatttccag
    10081 cgatgtttta ggtttcttag aatatacaga taaagctata tttcttgaca atcgtgacat
    10141 agttattcta gatcatcaaa gcataaactt atacgatttt aatggtaata atgtattcag
    10201 acctataact caggtagctt gggaattagg agatattgat aaaggaaagt acgcacacta
    10261 tacgataaaa gagatccatc aacaaataaa ttctattcct gtggcttttc agattgacag
    10321 aatatctctt aaaaaagcaa ccgatcttat ttcttcctct aaaaatattt ttataactgg
    10381 aagtggtaca agttatcatt cggcattatt agcaaaatat cttttttata aatttgcgaa
    10441 gataagaact gaaataataa tgtcaagtga gtttgaatat atgtctgcac ctattgatga
    10501 aaactcagta ctgatcgcta tttctcaaag tggtgaaaca gcagatgttt taaattccgt
    10561 taagatagct aaagagtata aagcaaaaat tctttctatc gtaaatatac ctacttcttc
    10621 tttgtctaga ttgagcgatc attatgtttc cataaattgt ggaccagaaa tgggtgttgc
    10681 tgcaacaaaa agctttactg cacaattggc aacaatatat tctataatag acagtttagg
    10741 tgtaaataac agatcattag acatgtcaac agatgtgttt aataatgctc taaatgagat
    10801 tttaatggat gattcctatc tagaaaaaat agctgaggat ataaaatctg tccaagacat
    10861 ctatatccta ggcaagtcct ttcattatcc aattgcatta gagggatctc taaaaattaa
    10921 ggaacttgct tatattcatg cagaaggtat agctgctgga gagataaaac atggaccttt
    10981 ggctatgatt gaacttaata caatcgtcat agttcttaat ccagaagatg aaacttatta
    11041 tgatacgtta catagtatac atgaaatgaa atcaagaggt gcaaagatta ttggaatttc
    11101 aaataaaaat aatgatatgt atgactattt tatcaaattg cctttagtta atgagaattt
    11161 ttatccaata attgaaatta tcccaattca aatattatcc tactacctgt cattaaaaaa
    11221 agatgctaat cctgactatc ctagaaactt agctaaatca gtaactgtga aataacttta
    11281 attcttaatt tagttgtatt ttcatagtca aatattaatt ccgtttaacg tgaatttttt
    11341 ttcaatattt ttctcatatt ctctctctat acatttcaac aaaatttctc cgttaagttt
    11401 tccatttgca tttatcattg ttgctattga tactcctcca gcaccaactc cttcttttac
    11461 aaagccattt gcaaatgctt ttagtcctag ttttgttgat tcaattaaat gtaaatccga
    11521 aataacaata cctacattgt tatctataga taatactagg ttagtaaaat ctgcgctatg
    11581 atcattaaaa atatattttg ttgtacctat gacgacttta tcagttttaa cattaagact
    11641 ttttaaaatt gataaaatag tacacatttg agttccacct gccagcatta ctcttccacc
    11701 attatcaata accccttgag cgatacctgt cactgatggg atcatagggt ctcctaacaa
    11761 agagatagcc tcaaaaggct tatctttcaa tgacccaaaa gtaatattat tttttttcat
    11821 actttttatt actatcatat tttttaaatt atgaggattt tctggcatac tactactcat
    11881 tttatttttt gcgtctatac ctaaagaaag cataaccgcc agtgcagttg ttgttccacc
    11941 aggaatgctt tctccaagaa ttacaagatc attactttta gatagttgtt taccgagcag
    12001 taatccatat ctataggctt tttctacctc ttctgactcc attgcaatgt tctccattat
    12061 atttttaccc gatcttagat taaatgaaaa taacggtata tcaggtttta tttttgctcc
    12121 tgaatctact acaataaaag gaatattacc aatgtttagc attgtttttg ttattatagc
    12181 aggagttggt tttccatctg gagtagcagg tacgccatca atacatttac atttaccata
    12241 ataaagaaac tctgcatcag ctgaagatgt atattttatt aaatcattat tagcccccgc
    12301 tacagtgata cctggaattt cacttgtttc agtatatgat attacacaaa cgaaaatagg
    12361 attgcttgat tctaatatat caatactgta tttgccgttt actactgtaa tatctttata
    12421 tttttctatc aatttgttat tctccttgag taataaatct tataaattca ttccttgatc
    12481 taggaactaa aatataatta cttttctttt cttcttctat cgtagaagtc ggtttgtatc
    12541 cataatcatg accgggataa acaatcaggt tattatctaa attcataatt tttttaaaca
    12601 aactgttgta catctcttca ggatcactac cgggtaaatc gatacggcca caatttccaa
    12661 caaataatgt atctccagta attattattt tagaatcaac tattaaacat atactatcct
    12721 tggaatggcc tggagtaaat aatacttgta caatggtctt tccaatcata aattcctctc
    12781 catcatttag tgatatatca tgtttttctc tagaattttt atgttgaaca actttacatt
    12841 tggtgtgaac aacaatttga tcgtttccaa gtatatgatc aaaatgtgaa tgagtgttaa
    12901 taacatactt tactttatat tcatttttat ttattatttc aaagatcttt tctaaatccc
    12961 atgacggatc gacaattgct gtttcttttt cttcattatc tataattaaa taagtgaaat
    13021 ttgccatttt tccaacagaa atttgttcta ttttaaaggt catgttatac tattcctctc
    13081 tcagcttcta taggtattcc tatcttcttc aatattaaat taccagtata ttttctgttg
    13141 ttcaacaatc ctattttaat tctatgaaat gttattgttg tattggccct tacacaatcc
    13201 gctgtaattt ttccattatt aggatctaaa ccagagggta tatccacaga gacaatgtat
    13261 gatttagatt tatttaccaa tttaattact gatagatggg gatcttgaat atcaccttta
    13321 attccagtgc caaaaattcc atcgataatt atatctgcat ttagtatttg tttttttatg
    13381 ttcgtcgtat ctaatttact atttaggatt aattctatag tattattcat ttttctaata
    13441 atttgaaaat ttgttaatgc ttcctctgtc ttaattgagg atgtttctcc tagaaatatt
    13501 actttaacat taatacctaa acaagttaaa tgtctcgctg caaccattgc atcaccacca
    13561 ttatttccag atccacaaat agctaaaatt tttaatttag aaactttttt atttttaaat
    13621 cttttaataa taaaatcagc tactccaaaa cctgcatttt ccatcattaa aacttttttc
    13681 ataccaaatt tagtatgtcc gatattttcg atttcataca tttgatgaga agaaatagca
    13741 atctctgaca tctttttgat gattttgtta tttttctcat tctttacttt cggatgattc
    13801 atttttgata tttatggagg tgtatttgta tttaatttga tatctttttt ttgaaaatgc
    13861 atcttgtaat tagaaaaatt gatttctaat gatccgttac tagtaatcaa aaatgttttg
    13921 ccgtcataat tacattttat ctgtggagtt tttccttttt tccatatcgg ctcaataatt
    13981 ttataatatt ctacaagttt tcttttttca taagataatt cattattaac ttttattctc
    14041 ttttttctca gaaacttaaa tgctaaaatt acatatttat aattataata tctaaacatt
    14101 ttaacaattg aaaaacatct ctctatttga tcgcttggga catacaatga ttctcctgtg
    14161 cctgatttag cttcaataac taacaaaatt cctttattgt tatttacagc tattatatct
    14221 ggaaggccaa cacttgaacc tccaagcctt ttggctatcc aattacgatt attaattcta
    14281 tttacaatcg aatattcata actatatcct ctgcttctac gaatgtttcc caagtatttt
    14341 tctattcaat attcaaactt ttattaattg ttggttttaa atagcatttt tattatcata
    14401 tcaaataatt aaaactctat attatgaaca ttaatcaaca aagatatggt aactgaaaaa
    14461 attgagattt cacttaaatc tataaacgat gaattgcatt caatagaaaa tagaagagaa
    14521 caattaataa aagaatcgcg agatgttatt attttgtgca gtcagtctat catatctctt
    14581 catcaaggcc atatacaaga aagtcaatta aaattagata aagcaaaaga tttgtattct
    14641 tctctcaaaa cactagctca ggtagatctt ttacgatatc tgtccatgtc agaacaagaa
    14701 ctagtagaat ctagtctatt gatgagtata gtaaaaaatg aacctctacc tggatcggat
    14761 gaaattggtg tttctagcca agcatatcta tttggaatat tagattgtat tggtgaaata
    14821 aagagaatgg tttatgatat ggttagactc ggcaggtatg agcaagcaga atcccttttt
    14881 agaatcatgc aagaaattta tagataccta tatccatttt ctatatatga taatatagta
    14941 agtggtatcc gaaagaaatt ggatatatgt aaaattttaa ttgaaaacac tagggaattg
    15001 ataacagaag aatcgagacg atcaatccta attgaatcat taaagaaatt agatagccgt
    15061 ctttaatcaa tatagtacgt ttgcaaataa tatggattat taagaaaagt taaatagaaa
    15121 ctatactaac tttttctcca tatggtatga gagatagtga aaaagtccaa aaaataataa
    15181 atgatgttac tcaaacagga aataaggtaa ttactgaaga ggatgcaaaa tctatattgt
    15241 cagtatatga gataaaggta cctgcatatt ccatcgtaac taccgaatct gaagctgtat
    15301 ccaaagcaaa ggaaattggc tttccacttg ttgcaaaaat agtatcagat cagatattac
    15361 ataagacaga tgttcaagga gtaaaagttg gcttacaaaa tgaaaacgaa gttaaagaaa
    15421 cgttcttaga tatgtataac cgtttgtcta aagactataa agttaaagga gtcctcttag
    15481 aaaagatggt tcctaaaggg attgaattaa tagtcggctt acaaaacgat cctcaatttg
    15541 gtccagtcat aatgcttggc ttgggaggaa tttatacaga attattcaaa gatgtatcat
    15601 ttagagttct tcccataact aaagaagatg tattaaatat gatagaagat ctcaaagcta
    15661 aagagatctt aaaagggttt agaggagcca agccagtcaa tctagaaatg ctttgtgatg
    15721 caattctcaa aataggtaat ttggggattg atatggctaa atatattgat agcatagatt
    15781 ttaatcccat tatagtttat ccagataatt actatgtcgt tgatgcaaaa attcttctga
    15841 aatcaattcc tgatgaaaat atcatctctc aatcaacacc tgattcaagt tatatgaata
    15901 cattctttga tacgaattct atagcattaa taggagcatc tcctgaagaa ggtaagatcg
    15961 gcaattctgt gttgaaaagt ttatctaaat acgactataa aggtaaaata tatccagtaa
    16021 atgctaaggg ttataatgaa attctagggt taaaagcata taaaaattta gaagaaatca
    16081 gtgaaaatat agatctagtt atagtcaccg tcgatttaaa atttgtaccg actatattgg
    16141 aaacatgtgg aatgaaagga atacataata tggtaatcat ttctggagga ggaaaagaac
    16201 tgggtggaga aagagcagaa atagaacaaa aagtgaaaga attatcttct aaattgaaag
    16261 ttagaattat tggaccaaat tgtataggaa tgtttaatgg tgaaaatcga ttagactgtg
    16321 cttttcaagg acatgataga atggttagac ctaaaaaagg agatgttgct ttcttatctc
    16381 aaagtggaac tattggaatt gcttttatgg aaacttcaga cacttttgga atgagcaaaa
    16441 tgatttctta tggtaatcgt tctgatgtcg atgaagcaga tatgatttgg tatttatcag
    16501 atgatccaaa cacaaatgta attggtctct atgttgaagg tcttggtaat ggtagaaagt
    16561 ttatgaatac tgccaggaga gttataaaag aaaaaaagaa acctatagta gtttttaaaa
    16621 atggacgaac ccaaagagga gctaaacaag cagcatctca cactggatcc ttgggtggat
    16681 catattctgt tgtcaaagga gcatttgatc aagccggtat tatatctgta gatagctatg
    16741 aggaattaac cggatcatta aaagctttaa cttggcaacc tattcctcaa ggaaataaag
    16801 tggctttagt taccaatggt gctggaccaa taattgctgc aatagatcac tttgaaagat
    16861 taggattaaa aattgctgat ttaagcgaag attctataca caagtttaat gaacattatc
    16921 ctgcaacgta tgttataggt aatcctcttg atgtaacagg ttctgccaat gctgatgatt
    16981 ataaattcgc tattcaaaca tttatggacg atcctaatgt tgatattata atgccttggt
    17041 ttgtttttca agatgatcct cttgaagaaa ctattgtaaa tatattagga gattttcaaa
    17101 aacaaaagaa aaaaccaatt ttagtaggtg ctttaggcgg accatttact attgatatct
    17161 caaacagatt agaaaaaaag atggtaccag tatatcattc agtactatca tgggctgtag
    17221 cagctggtgt actcgcaaaa tggtcagagc ttaaaaatag aaagtcttaa tgtctttcaa
    17281 gacagggaaa acttatattc tttttcaaca taattaaact atggaaagta cacaacaatc
    17341 acaaatagta actgttactc caaaagccgc agaaaaagtt agtgaattta tgaaacaaga
    17401 aggcaaagat agtttatttc tgcgtgtata tgtcactggt ggaggctgtt ctggcttatc
    17461 ttatggtatg ggttttgaag aaaattctga agaagatgat cttgttatag agcaaaacgg
    17521 agtcaaaatt gtggtagaca attacagtca aagatatttg aaaggagcta atattgatta
    17581 tattgatagc cttatgggtt ctggattcaa aattaataat ccaaatgtaa ccaaaagctg
    17641 ttcatgtggt cattctttct ctactgagta ataccatttt tttaattttt tcaatatttg
    17701 cttcagacaa agttatttat taagtagagt aaaaaattaa aaatataata gatatattat
    17761 ggccataacc ctctgatatt aattgcttca acaaccctct gtactgcaag taaggtagcg
    17821 gcttttcgca tgtctacatt gtattttgta tgaatatcat aaacatcata gaatgatttt
    17881 gtgatgttta tatctaatct attattaact tcttcttctg tccaatattc tcttcttaaa
    17941 ttttgtaacc attcaaaata cgaaacagtt acacctcctc cattagcaag tacgtccgga
    18001 atcactagaa tatctttttt aaataaaatt tcatctgctt caggagttgt agggccgtta
    18061 gctgcttctg ctatgatctg tgtttttatt ttatctgcat tcttcttagt aatttgattt
    18121 tccaatgctg ctggaatgag aatagtacac tctatttcca aaagttcatc attagtaatt
    18181 gatttcgcat caccgaatcc tactatactc cctgtttctc ttttaaattt cattaatgca
    18241 tccacagata ttccatctgt cttaattatt gcaccttgag aatcggaagc tgctatcatc
    18301 tttgcacctt gctcttcaac tagttgggaa gaaaattgtc ctgcatttcc aaaaccttga
    18361 acgacaaatg tagcttgttt catgtcaata tttatttttt tagcagcttc tctgacggta
    18421 tatgccaaac ctctaccagt tgcctcagtt ctgcctaatg aaccaccaat tgctattggt
    18481 tttccagtta ttacttctgg ttgaacataa tttcctttta atgcagaata agtatccata
    18541 atccatgcca tttctttacc tccagtatat acatctggtg caggaatatc cctatatgga
    18601 ccaataatat cagctataag gtatgcaaac cttctagtca ttctttctaa ctcattttct
    18661 gacatctgtt ttggattgca aattattcca ccttttccac cgccgtaagg aatattcgct
    18721 attgcacatt tccatgtcat ccacattgat agagccttaa cttcgtctag tgtaacatca
    18781 gggtgatatc ttattcctcc tttgtgaggt cctctggcat cattatgttg ggatctaaac
    18841 cccgtaaaca cctttatcga accatcatcc attcttgttg gaatagaaac agttagaatt
    18901 cttttaggat tggataatat ctcaaacatt ccattgtcta aatttagaat ttttgatgct
    18961 tcttgtagtt gttttagtgc tacttcaaat gggtttattt tttcatttaa attagatgaa
    19021 gacatatatt ttattcataa tgtgttgttt aatcttagat aataaatata tctctaaaaa
    19081 ttatcatttt tcatcttttt caagataatt aagcgaagtt gctcgtcaga tatatccaaa
    19141 taatcaatat tgagtgatgc cgctattttt tctagttttt ctctttctgt tgacacagga
    19201 ccgtgtatta tctttccaaa atacaaattt ttttcgttat taagctcatc atcaaatgta
    19261 ctattactgg ctttaacttt atttacttgc ttaagaaggg tttgcctggt attattatat
    19321 tctccaattg ctttacctat atgtcttgtc atatctggta attttcttga accaaaaagt
    19381 aaaaagatag taactaaacc tattattacc cattctgatc cgccaattcc catattatct
    19441 ataataaatt aaagataatt tatttattaa ttatgcattt ataaaaaggc gtaacaataa
    19501 ttatttaaaa ttacttttaa aaaagaatta caaatttaat aatgatacat tcagatattg
    19561 gtcaaagcac ttatattcgt gccaatcacc cctaatttta ctttacaata aacatgaggt
    19621 tatggaattg cctaatactg ggatagtaaa atatcacgtt aaattaagtt tcgacgtaga
    19681 tggactcgta gaaaaagcgg atataatagg tgctattttt ggccaaactg agggactatt
    19741 aggacctgaa atgaatttga atgaattaca aaaggtctcc aaagttggtc gaatagaagt
    19801 caatgtggaa acgaagtcga atatagcaaa gggtgatgct cttattccta tgagcaccga
    19861 tatttctact gctgctctta tagcggcggc aatagaaagt atagataaag tgggaccatt
    19921 tcaagcaaga ttcgagttgt ctggaattga cgatattcgt gccataaaga aaaaagttat
    19981 agtagataga gcaaaaaaaa ttgtgcaaga atgggctact aaaactatta gcgaaggtga
    20041 agaaatgctc aaagatgtat atgatgcaag taaaccaggg aaacttacta catttgggaa
    20101 aggtcaactt gcatgtggga caggtgtatt caatgctgat tgggttattc tagtcgaagg
    20161 ccgagctgat gttattaatc tgttgcgagc aggattcgat aattctatag ctatagaagg
    20221 tgctaagatt gatgaaagta taactcacct tactgaagga aaaaaagtta tagctttctt
    20281 agatggtgat agagcaggtg atttaatttt aaaagaactc cacggcttag ttaaaatcga
    20341 taaagtattc agagcacctg ctggaaggga ggttgaagaa tgtacacctt tagaaataac
    20401 tgaaatatta aataatgcag tggacgatcc aaaagaagac tcattctcaa ctataaaaaa
    20461 acaacaatct gtattacaaa atcataaaaa atataacaaa aacgacagag aaaaaacttc
    20521 ttttcagcag acccaacccc aatttaatca cttagaaacc tatccaatta ctaaagaaga
    20581 taatggatta caaactaata atgaatccaa gtcagttatt agaaacatat attctcaaat
    20641 caatgaaacc ttagaagcaa ttattctgga tggttcaatg aatactatta tgaaagttcc
    20701 agtttcggag atagttaaaa aactttcaga ttcgtctgga ggtaaaattt tggttctaga
    20761 tggaatagta acccaaagat tgattgatgc ctctgataaa gctggtatag aacaaataat
    20821 aggtcataga ttagcggatt taaaacgtag ttcatctatt aaggtaggta cattcaatga
    20881 aatgggatta caataattag attactaaat aattttatga atgatat
//
LOCUS       DASVEV010000038         6968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1368772, whole genome shotgun sequence.
ACCESSION   DASVEV010000038 DASVEV010000000
VERSION     DASVEV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368772"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VF222_01900"
     CDS             <1..600
                     /locus_tag="VF222_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX7141210.1"
                     /translation="AQAQQQQSSKEAPEKGTIFVFNEKSRPANEPEKCAALASVVKGK
                     ALPDYNLCDIVVYRQAPPIVRNDGMVLNNFSGLGHYIEMVPAVVNNTGVFNSKDTPSN
                     QTSTSPQKQDNLKVAFGEWSVIDEEVVPVQQVMQKYNWTQTALHHHMLNESPKILFLH
                     WSVTGDANELIKQAKEIIMQTSTYQNLTGNTRTPTSGGP"
     gene            735..917
                     /locus_tag="VF222_01905"
     CDS             735..917
                     /locus_tag="VF222_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141211.1"
                     /translation="MPIELVVSEIIENAFNIDFNTIFAVTYRTAIVTLLIVFVIKWLG
                     NKGLGRLGSIEIIIIL"
     gene            942..1235
                     /locus_tag="VF222_01910"
     CDS             942..1235
                     /locus_tag="VF222_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7141212.1"
                     /translation="MLNPTEVSIPQGFMVIIIAIAIFKMYDYLTTRYKRFSKVIVAKP
                     ILLVKDGKILKDSMMKARISEDEFVSYLRLSGTNDISDIKLSYLEINGQVLSK"
     gene            1302..1556
                     /locus_tag="VF222_01915"
     CDS             1302..1556
                     /locus_tag="VF222_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141213.1"
                     /translation="MDTILKSIIKRQIPFLIGGTITGSIMAYYYGFLFTIIVNSIMWY
                     VISYLVSKYYYKSKGINDQKYLIHYGLSKIKSRKKRVKTS"
     gene            1795..2241
                     /locus_tag="VF222_01920"
     CDS             1795..2241
                     /locus_tag="VF222_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141214.1"
                     /translation="MLYIYERIKFSLIMIGDLLYEGKGKLVGIRLLNAEEYKIEHSVL
                     EEGKFKDIEITLIGTFWTIPSGKNVTYVEGQGIITTKDNEDTATFRGYGIGTSKGQVS
                     VSTRGSVIYKSSSTNGKLSYLNNKIGVFEGEVDESGTSSEKHWEWK"
     gene            2908..3474
                     /locus_tag="VF222_01925"
     CDS             2908..3474
                     /locus_tag="VF222_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7141215.1"
                     /translation="MQNQNRTNKNRSALRILQIVLGIISVIISGLVISHIFIAVGFIT
                     TLLAIALLAIGIERLGMGILSRSSSKSSRISNIILGALTIILSIIVIAYPSITTSFIL
                     VLLSVSLIVIGIARIISTIKDKTKGWKKNLMIGIGILSIFVGGMIIANYVFGLFLISL
                     ILGISLLINGVESIILGASGKRKENHLK"
     gene            3844..4707
                     /locus_tag="VF222_01930"
     CDS             3844..4707
                     /locus_tag="VF222_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141216.1"
                     /translation="MNNKKMLLASVLLVATAAAGLLALNPSIMIGNAQAQMYEDEYYD
                     DSYYKDDNRYGYDNGYGYDNGYLEKDKNPKIFPANKVSKLGDRWWQWAFGIDTTDVNP
                     FTNLGQDGCDIGLQYNGRLLFLVGSPRDPNTGDFPVHECDVKKGTSILFPILNVACNN
                     LAVDTPFFGANEQDQRICENKIITDSTLFDALHVNIDGIEVQNLEQYRIDSPGGGFEF
                     TAVQNNVFDTPVGDGNGVSDGFWILLEHLKPGKHTISFSGAIDLDNISPLMGLYEAGA
                     TYNLNVKSGYY"
     gene            5448..6389
                     /locus_tag="VF222_01935"
     CDS             5448..6389
                     /locus_tag="VF222_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141217.1"
                     /translation="MHKSAAITISTLMMASLIMLVPFASTNTYTNPAMAQEYGKYGDN
                     RNSKYPIDDKTYECRTGPFEGFLVSSVEFCDAKHKFNDYNKVKIFPPPGILTAEWWQW
                     ILDIPKEINPLLDETGENCDVNQQGPVWFLVGTPGDSETGGIITGSVERDCVIPEGKK
                     ILFPIINTFCSELNDQAAIREALGIPTGQPIPPSQLEEGLRLCAEDIINGVNITEASI
                     DGKEITNFENFRVQSPLFTISYPEDNPFNVTDFIEIPQKAISDGYWVLVKGLEPGEHT
                     IEFRGGISGVFETEVTYHITIEPTNNNDNNNYYDNSY"
     gene            6754..>6968
                     /locus_tag="VF222_01940"
     CDS             6754..>6968
                     /locus_tag="VF222_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141218.1"
                     /translation="MSNGTGPKSSSKKPTKKITTAAKEAKATKSVPITVELNILKVLI
                     NERPLIPLFRILKKNKELHTRELLITMG"
BASE COUNT         2578 a          990 c         1089 g         2311 t
ORIGIN      
        1 gctcaagctc aacaacaaca atcatcaaaa gaagcaccag aaaaaggcac tatctttgtt
       61 ttcaacgaaa agagtagacc agcaaacgag ccagaaaaat gtgctgcctt ggccagtgta
      121 gttaaaggaa aagcactgcc tgattataac ttgtgtgaca tagtagttta tcgtcaagct
      181 ccacccatag taagaaatga tggaatggta ttaaataatt tctcaggact tggtcactat
      241 attgaaatgg taccagcagt tgtgaataat acaggagttt ttaattcaaa agatacaccc
      301 agtaaccaaa catcaacatc acctcaaaaa caggataatc taaaagtagc atttggagaa
      361 tggtccgtaa tagatgaaga agtagtacca gttcagcaag tcatgcaaaa atataattgg
      421 acacaaactg cattgcacca tcatatgtta aatgaatcac caaagatatt attcttgcat
      481 tggtcagtaa ccggtgatgc taatgaattg attaaacaag caaaggaaat cataatgcaa
      541 acatcgacat atcaaaacct tactggtaat acacgaactc caacatcagg tggtccatag
      601 cataaacggc taaataatat atttaaatct attagatgct tataaaatcc tcatcttcat
      661 ttttcattat ttaattgcaa atttatttat tcagtatagc attataacta gcataactag
      721 tttttttatt ttatatgccc atagaattag tagtatcaga aattatagaa aatgcattca
      781 atatagactt taacactatc tttgcagtta cctacagaac agcaattgtt acccttctaa
      841 ttgtatttgt aataaaatgg cttggaaaca agggtttagg tcgattaggt agcattgaaa
      901 taataataat tttatgagta ggaaatgcag ttagtgaacc catgttaaat cctactgagg
      961 tctctatacc ccaaggattt atggttatta ttatagcaat agcaatattc aagatgtatg
     1021 attatcttac aacaaggtac aaaagattta gcaaagttat agtagcaaaa ccaattttgt
     1081 tagtaaaaga tggaaaaatc ttaaaagact ctatgatgaa ggctagaatt tcagaagatg
     1141 aatttgtatc ctatttgagg ctaagtggta ccaatgatat ttccgatatt aaattgtcat
     1201 atttagaaat taatggtcaa gttttatcaa aataaaggaa aaagactgaa aaactgctgc
     1261 tattactact attataggat gttgtctatc tttaatgtag tttggataca attttaaaat
     1321 caataataaa acgacaaata ccttttctaa ttggtggtac tattactgga agcattatgg
     1381 catactatta tggtttttta tttactatta tagtaaatag tataatgtgg tatgtgattt
     1441 cctatctagt tagcaaatat tattataaat ccaaaggaat taatgaccaa aaatatctta
     1501 ttcactatgg tctatctaaa ataaaatcca gaaagaaaag agtaaagact tcttagatag
     1561 ttcgttattt ttatatgact taatttgata gctatgtttt ttaaaattaa aacatcatct
     1621 agtttgtaag gataaggaac ttcataaaat atagatattt agatatgccc taattcctaa
     1681 gactcattat cattgttgca tcattctttg aaagttctct taacacatta tacaaatacc
     1741 tactttttcg tagtctaaat ttccatctga tattttaagt agttttaata gattatgctt
     1801 tatatatatg aaagaataaa attctccctg attatgatag gagacttatt atatgaagga
     1861 aaaggcaaac ttgtaggtat aagactctta aatgcagagg aatacaagat tgaacattct
     1921 gtgctagagg aaggaaagtt caaagatatt gagattacac taataggtac attttggaca
     1981 attccttcag gtaaaaatgt aacttatgtt gaaggacaag ggataataac caccaaagat
     2041 aatgaagata ctgcaacatt ccgtgggtat ggaataggaa cttctaaagg acaggtcagc
     2101 gttagtactc gtgggtctgt aatatataaa tcatcttcaa caaacgggaa actttcttat
     2161 cttaataaca aaataggagt ctttgaaggt gaagtagatg aatcaggtac ttctagtgaa
     2221 aaacattggg aatggaaata aaataatata aaatctaaaa tttttttatg tttgtaccac
     2281 aaaaccaaaa tatcattcac aaagttaatg agaatcttca ataacactga atgtaaatgt
     2341 agtatgtttt gataatccac cattgcgacc ttaactttcg ttttttctct gaattttaac
     2401 taatctatta aatatgtatt aataactgaa aaataaatat taaatatttc cgtctttatt
     2461 actataactt agactcattt tagaaccgct atcggtatta ttccctcctt tgcaaattat
     2521 gctgttattc tcttcctctt gtaaatcgta taatactctg taaatctctg aatccagttc
     2581 cttacagaaa aataactatt accacaattg ttgaatagtt tcaatgcctt gagtgtcttt
     2641 agcaggctaa gaccaagaaa gtacaaatac aatgagtaca taacaattaa aaagattaag
     2701 tgaatatttc tatatcatat caaagtgtat agaaaattta ttaattgatt acatgtatat
     2761 agaataaatc ccttcgctga aattaagata tgtattgaac tcaataagtc tctagttaat
     2821 atgcttaatc aaaatctaca cggacaaagt ataattcata agagctttga gagtacctta
     2881 aataattgac tagattccaa tatttttatg caaaatcaaa accgtacgaa taaaaatcgt
     2941 tcagcattac gtatcttgca aatagttctt ggaataattt ctgttataat ttctggtttg
     3001 gttatttcac atatctttat agcagttgga tttattacaa cactactagc aattgctctt
     3061 ttagctatag gaatagaaag attaggaatg ggaattttgt caagatcatc tagtaaatca
     3121 tcgaggattt ccaatataat actcggagct ttgacgataa tattgtctat tatagtgata
     3181 gcatatccaa gtataaccac atcattcata cttgtattat tatctgtttc attgatagtt
     3241 attggaattg caagaatcat ctctactatt aaagataaaa ctaaaggatg gaagaaaaat
     3301 ttgatgatag gaataggaat attatcaata tttgtaggag gtatgataat agccaattat
     3361 gtatttggat tgtttcttat ttccttaata ttggggatat ccttgttgat aaatggagta
     3421 gaaagcataa ttctaggagc atcaggcaaa agaaaagaaa accatctaaa gtaatattcc
     3481 aagacggtag attgaaacta aatgattatg attcattagt tttactaata caatttttct
     3541 aatttatcaa gtcaataaat gtttgtatat attattctaa ttcatatcaa agaagtttag
     3601 attattttgt ttactttttt agatttataa taacataatt tattttatag attttttgta
     3661 tatctattgc tctagtgata ttacttgtcc ttgttgtctg gttttatctt agcgatttca
     3721 tctagtaact aaacatcaat tgctattctt cttattgttg ttaatttaga aacttggaat
     3781 attacaagaa cagataatgt tccttccaat catagatatg ttaaattcgt aactaacttt
     3841 gtattgaata ataaaaagat gctgttggct tctgtcctat tggtagcaac agcagcagcg
     3901 ggtctactcg ctcttaaccc atccatcatg ataggaaatg cacaagcaca aatgtatgaa
     3961 gacgaatact atgacgatag ttattacaaa gacgacaata gatatggata tgataatgga
     4021 tatggatatg ataatggata tttagagaaa gacaaaaatc caaaaatatt tcctgctaac
     4081 aaagtatcaa aacttggaga tagatggtgg caatgggcat ttggaataga tacgaccgat
     4141 gtcaatccat ttaccaattt aggtcaggac ggttgtgata taggtttaca gtataacggc
     4201 agattattgt ttcttgtagg ttcaccaaga gaccctaata ctggtgattt tccagtacat
     4261 gaatgtgatg ttaaaaaggg aacatcaatt ctatttccaa tattgaatgt ggcctgtaat
     4321 aatcttgcgg tagacacacc tttctttggt gcaaatgaac aagatcagag aatatgtgaa
     4381 aacaaaatta ttaccgattc aacattgttt gacgccttgc atgttaacat agatggaatt
     4441 gaagtccaaa atcttgagca atacagaata gattctcctg gtggtggatt tgaatttact
     4501 gcagttcaaa ataatgtatt tgatacacca gtaggtgatg gaaacggcgt gtctgatggt
     4561 ttctggattt tattagaaca tttgaaacct gggaaacata ctatatcatt tagtggcgca
     4621 atcgaccttg acaatatatc tcccctaatg ggtctttatg aagcaggggc aacatataat
     4681 ctaaatgtaa aatctgggta ttattgacaa cgtcggaaaa tatagaaaaa tatttaaaag
     4741 aaatattttt aaatattata aatttatttt taattttata gattaattaa ttaataaatc
     4801 tattacttta gcgcatttct tgtatcaatt aaattgttat tgaacactga aatcgtgtct
     4861 attcaatttc tgaagcgtat agttttttca atgaaatatt ctttatccat tttctatagt
     4921 taaaatagtt ctaacaataa aagctaaata aggaaagtag aaccttactc catggacgaa
     4981 ttcactcccg accaactttc tttaagacat agatgaatcc tactattcta aagtggaacc
     5041 cgattaatgt cattactatt agaattataa tgagaagata gggcaaagaa tataaaaaat
     5101 ttatctaaag gaatttaaag taacttatca ataatctgaa gaatgggtat taacaccttg
     5161 cattaacatt ctccagatat gtgtggaact gattccagta aaaacattat ataatattta
     5221 ttatagttta ctacaactta ttctcagttg atattatata acaaattgga ctaatataca
     5281 cattatcttt tgtaaaatta agcaagtaca acatttttca ctattgttat tataaaaaaa
     5341 ccagctattg aataatataa ataataattc cctaagtaaa atatttaata tatgttactg
     5401 aatgattctt cgaattctaa atataattag aaagttagtt aacaaatatg cataagtcag
     5461 cagcaattac tatcagtaca ttaatgatgg catcgttgat aatgttagta ccatttgcta
     5521 gtaccaatac ttatacaaat ccagcaatgg ctcaagaata tggcaaatat ggcgacaata
     5581 ggaatagcaa ataccccatc gacgataaga catatgagtg tagaacaggt ccatttgaag
     5641 gattcttggt aagttcagtt gaattttgtg atgctaaaca taaattcaat gattataata
     5701 aagtaaaaat atttcctcca cctggaatac taacagcaga atggtggcaa tggatcctag
     5761 atatacctaa agaaattaat cctttgcttg atgaaactgg agaaaactgt gatgtaaatc
     5821 aacaaggacc tgtctggttc ttagtaggaa caccaggtga tagtgaaact ggcgggatca
     5881 ttacgggctc cgtcgaacga gattgtgtaa tcccagaagg aaaaaagata ttgtttccaa
     5941 taattaatac attctgttca gaactaaacg atcaagctgc aataagagaa gcattaggta
     6001 ttccaacagg tcaacctatt cctccatcac aattagaaga aggattaaga ctgtgtgctg
     6061 aagatattat taatggagtt aacataacag aggcttctat agatggtaaa gaaataacta
     6121 attttgaaaa tttcagagta cagagtccgt tatttacgat tagttatcct gaagacaatc
     6181 cttttaatgt aactgacttt attgagattc cacaaaaggc tatttctgat ggatattggg
     6241 tcttagttaa gggacttgag cctggagaac acactataga atttagagga ggtatttcag
     6301 gagtatttga aacagaagtg acataccata taacaataga accaacaaac aacaacgata
     6361 ataataatta ttatgacaac agctattgaa gttataataa taactattat tattaatcca
     6421 tttcaatatt ttttacttag ccagaatatt tagactcttt atttagtaat tatctaacca
     6481 aataacttat tttgaaattt ttagtatttt gataatttct attttaaagg aactaagaat
     6541 gtcatccaac taatcttatt aacaaaactg tgtcatgtta ccgttccaga tgttgccatt
     6601 aaactactaa ttttttatat tatctaaatt ttgttgtgtt tctaattcca tattataatg
     6661 taattttaat tggtcaagga gttacttatg agaaaattaa tctgacatta ataacataag
     6721 tttttattat tatttaaagt taaagtaaat aatatgtcta atggtacagg tccaaaaagt
     6781 tcttctaaaa agcctactaa aaaaatcaca acagcagcga aggaagcaaa agcaacaaaa
     6841 tcagttccta tcaccgtaga attaaacata cttaaagttc tgataaatga acgtcctctt
     6901 attccattat ttagaatact aaagaagaac aaagagttgc acacaagaga attacttatc
     6961 acaatggg
//
LOCUS       DASVEV010000039        34054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_786419, whole genome shotgun sequence.
ACCESSION   DASVEV010000039 DASVEV010000000
VERSION     DASVEV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 34054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34054
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786419"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1055..1552)
                     /locus_tag="VF222_01945"
     CDS             complement(1055..1552)
                     /locus_tag="VF222_01945"
                     /inference="COORDINATES: protein motif:HMM:NF018392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoantigen p27 domain-containing protein"
                     /protein_id="HEX7141219.1"
                     /translation="MSNGKSADEKEKKENNHQGRNNIKSAADLLLKGGTLLSSSCAKC
                     GGVQVRYKEETVCVSCGRKIKEMSNDLQQMEEEQQQQEQQQQHSIPDSNLSKESKVPT
                     IFDAHITNISYTKILTDKINFLISTLENDNDILIQDTKLDLISKYLEVLDKLNIISRS
                     NSGNK"
     gene            complement(1683..2423)
                     /locus_tag="VF222_01950"
     CDS             complement(1683..2423)
                     /locus_tag="VF222_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7141220.1"
                     /translation="MQQEHHHDQSQETTTIKSSIDELSKKWNIEKPIYDLHLGRRNDV
                     VDTTVKVNQVVFHIPKLSNDGLFVLWNCLWPDCHNCCEKQGRLPLTKDDLYTLKNKLN
                     YKSLNEFLDKETKVSSWNEPGSYGNLITHLTMISLKRKVNETDEEDGTPIKCRFLDDQ
                     GSCQIQEGKPGVCWLYPFASWMESYKNRPLVHATFQFTGDCPGFYLSKTINDMLPVLQ
                     EYSLKIYNYNMSTNRTIREGFGATNIIY"
     gene            complement(2576..4462)
                     /locus_tag="VF222_01955"
     CDS             complement(2576..4462)
                     /locus_tag="VF222_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HEX7141221.1"
                     /translation="MEFTNFDNEQSSQNIMATILKSLPKESGLTKIEYEGPSIALYSK
                     NPRFLIENSQILSNMVNMIRKRVVIRTDEAIRKSRNNSTNIIQTRLLPLKVEINGIIF
                     DDALGELTIYINNSFETKNQIENTILLDLVTETGWKIKIRKSTVKLSTIQFIDKVLDN
                     STDYRIKFYKEVGDKVFRPKLSNSCEASILTLGGFGEIGRSCILLVTKESKILLDCGI
                     NIHEDKAINHFPRFDMTGFTLDEIDAVIITHAHIDHTGFLPFLFKYGYRGPIYCTEPT
                     LPLMILLFLDYHKNHEIYSEEDIKNVIIHTMPLSLGIVTDISPDVKLVLGNSGHILGS
                     SSIHLHIGNGNHNLVYTGDLKFGKTNLLENASWNFPRVETLIIEGTFGSKDLPHKREE
                     TDPILIESINQTLRDGGRVLIPVPIMGLSQEIIYTLTKYQEGGSLDPSAEIFVDKSIL
                     EATYMHELYYDYLSKEVKTKILNENISIFNYKNIKLLNSKSKVEKPGIILVPSSMLIG
                     GLSVKFLKQIHNDPLSKIIFLSNQGTGTLGKEIQSGNTTEVTINGQQLKLKCKIHSIH
                     GFSNHSDYNQLLAYISRLKPKLKRVIINHGEKPKVQNLASSVNKILNIQCQYLLVPEA
                     IKLL"
     gene            4615..4920
                     /locus_tag="VF222_01960"
     CDS             4615..4920
                     /locus_tag="VF222_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX7141222.1"
                     /translation="MDVESNGENTRNIPTENNSGNETGNSGSDTGFRKFRSFRIPVKV
                     GEEYDVKIEAMSKRGDAGVAKIEGMVIFVANTKEGDEVKIKITRVGMGYAIADVVQQ"
     gene            5033..6091
                     /locus_tag="VF222_01965"
     CDS             5033..6091
                     /locus_tag="VF222_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HEX7141223.1"
                     /translation="MKSNSHLMELPRKILVGDNVISDLGNFITDINSEISNVVIISGE
                     TVRNKIQDNIKRSLEKYRIKNYWFIRKEASFETVSNIKIEIEEIEPDIILGIGGGKSV
                     DVGKMIAYSIKKPFISIPTSASHDGISSPFVSLKGSNKPHSIKVNTPIGILADIKLIS
                     EAPSRLLSSGCGDLIGKLTAVKDWELARDDKNEYFGAYSAYLAKLSADIIMNKSKELL
                     LNEYGVRTIIEALISAGVAAGIAGSSRPCSGSEHLFSHALEYITDGHCGLHGERVGLG
                     TILMSKLYGMDFEGIIAVLENVQAPVKADQIKLTEKDVVQSLLIAQDLRPERYTILSK
                     TKLDKKSAYELAKSAKVI"
     gene            complement(6230..7057)
                     /locus_tag="VF222_01970"
     CDS             complement(6230..7057)
                     /locus_tag="VF222_01970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7141224.1"
                     /translation="MPFDKGSLILLDYTARIKDNGEIFETTIEDEAKKSNLHDPTRTY
                     EPRLISVGEGWVLKGLDEALTSIDVGQKLSIEISPDKGFGERDTNKVRMIPQRKLGEK
                     ANEIKVGDVVELDDRTGIIRYIGSGRVQIDYNHRLASRVLVYDVNVVKKVESNDDKIK
                     YLLKRRLPIDDEKAKFEFKGDTVIIELSEDISLLDGLQIIKKAVTSDIFKFVDGLNKV
                     TFQEVYLSQKTKESSPQTSTANETETSASANETETSNTSPRSDTSLRSESVSTSENK"
     gene            complement(7116..8258)
                     /locus_tag="VF222_01975"
     CDS             complement(7116..8258)
                     /locus_tag="VF222_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX7141225.1"
                     /translation="MEYLVMLPGPTNVPNRVMNAMLAPVINHRSEDFRILYKGIIEKT
                     QKVFETNGDIILFTTSGTGAVESSVVNLIKKGDKAIIPVNGEFSNRLADLIDSWGGTA
                     IRIDAPYGQNPPFEKIEEAFEVNKDVKALYAVYNETSTGTTIRYMDKLGDLCGKYGSF
                     FIADAVSILGGDELPVDKWNVDICITASQKALAAPPGVSPISINERTKKYMQSNPAPI
                     LYLNINRYFKYYKEHTETPFTPALPLYYAFDEALNLVLEEGIDKRIQRHRQCANAFYA
                     SLSALGLTPFAKEDAKSNVIIAFNYLEGMDDKRFRTLLSDEFRVLIAGGFGNLKGKVF
                     RIGSMGEVTRYHVMRTVSAIYSAMKMLGINANDKALSVAEEKLKNL"
     gene            8351..9442
                     /locus_tag="VF222_01980"
     CDS             8351..9442
                     /locus_tag="VF222_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7141226.1"
                     /translation="MFTTRKRKLLENVSKINCKNILSFQPENIFYLTGFWGEGVVLMN
                     HNTTKLFTPKLEYFRASEDSKECEIIKTERGSDNILLSTISELDNNGFYCTDNANYNL
                     INQLKEKAGFNKIIVDEGPFFKTRSIKDEMEIKYIKQAANIIDKLYDICLEEIKEGVS
                     ERQLQAILVYETFKNGGGFPASKSTLNPFIIASGPNSALPHASISDRKFKRGDFIVVD
                     LTLRVNGYVADATRTFGLKTINPELKKIYEIVKDAQVLGLTKCSNKIKIGEVDKTCRE
                     YISKNGFETEFNHSTGHGIGLDVHEPPWIRPNNEELLMDNMTITIEPGIYLESKFGVR
                     IEDSIVIDSKRNRTGIINLNTFEKNLIII"
     gene            complement(9545..10807)
                     /gene="coaBC"
                     /locus_tag="VF222_01985"
     CDS             complement(9545..10807)
                     /gene="coaBC"
                     /locus_tag="VF222_01985"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7141227.1"
                     /translation="MTSDLHPSKDITNTLGTTLKDKKIVLCVCGSVAAYKAIDFARLL
                     MRHGANVFPVISRSATMLLHPNYLRWATGNDVVDKLTANMEHIELADFNKSDLIILYP
                     CTANTIGKFANGIDDTTITSILSVGLGSKIPIFIAPAMHEAMYSNQLVVENILKLKKL
                     GVIFLDPEITEGKAKILQPDQALKSVLEFFTKSKKKDLFEKNILVTSGGTMEYIDPVR
                     VITNLSSGKMGNAIIKESLELGARVTHVVGHSSVLCSSNSSNLETIKVNTSDEMYTQV
                     ISRIRSKKFDLVILAAAVTDFKLPQIYKKKIPSQISRPINLELVPTKKIIDEIKLIDK
                     NIFLVGFKAYFDVSNNYLITKAKKKLKECNADLIVANDVGRIKTTIGSNYNEVFIVTQ
                     KEPVVHLKVQNKDSIAKKLFEIIIAKKK"
     gene            complement(10821..11738)
                     /gene="panB"
                     /locus_tag="VF222_01990"
     CDS             complement(10821..11738)
                     /gene="panB"
                     /locus_tag="VF222_01990"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7141228.1"
                     /translation="MTTTNNNSKNNNNHYHQKPRLKKLVVNDIVSMKKREKISVITSY
                     DYWSAKICDSTDIDIILVGDSGGMVMLGYNSTIPVNMKEMLIFSKAVSVGTKRSLIVS
                     DMPFGSYQIDINHAVKNAIRFLKSGSDAVKLEGGIEIIDRIKAIIKVGIPVMGHIGLK
                     PQTTKLWEGYKVQGKTALEGSQLLREAIELEKAGVFAIVLEMVTSEVAEFISKSLKIP
                     TIGIGSGPHCDGQVLVLHDMLNLYDNIKPRFVKTYTNLSETILNALHNYITDVKTGKF
                     PEDKHSYKIEDKEYERLLKTIKRFRKKLS"
     gene            complement(11738..12499)
                     /locus_tag="VF222_01995"
     CDS             complement(11738..12499)
                     /locus_tag="VF222_01995"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HEX7141229.1"
                     /translation="MFDINHIPSNHPRAKSLAIRQLLTNGFKSGLVVSEGLIAHGRGE
                     AFDYLIGEKTSKYALEAIQAASSLLLLAQRPVISVNGNTAALCSKEIVDLAYETGSSI
                     EVNLFYRNKKREYLIAETLKQNGANVVLGVGQKEFLKIPELTSNRRHVDPEGIFLADV
                     VFVPLEDGDRTQALINMHKKVITVDLNPLSRTAQNATISIIDNIVRSIPELVKASKQL
                     KQLSQNSLLEILNNFNNKYNIDNSLKIIRGWKCEQ"
     gene            complement(12492..13427)
                     /locus_tag="VF222_02000"
     CDS             complement(12492..13427)
                     /locus_tag="VF222_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141230.1"
                     /translation="MTTSEIGELVASCTAFSPGHITGFFEKPENTNQNSDVLFYGSKG
                     AGFSFKKGVTTSVELYKSKNNWYQIYINNSLTNNAEVSKWIVQYYLDKFDFTYYIKIN
                     HSIDIPIGYGLGTSGAAALSLSYALNKALDINFLKEQAAQIAHIAEIECKTGLGTVIS
                     EYYGGLEIRTSFGAPGIGKVTKIELNNYKAIILCISPLSTKQILTNYSNNANTLGKKM
                     VEQLLLSKDVPSFLRMSREFADFLGLTKGLCAKPIEELNHFGVDCSIGMFGETIFTLV
                     PPNEISKVTSILKKFQGTLIVSDIDNRGALIVDFV"
     gene            13681..14484
                     /locus_tag="VF222_02005"
     CDS             13681..14484
                     /locus_tag="VF222_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7141231.1"
                     /translation="MSKDIEIICIGNEILNGTTIEKNSCWLATRIRQIGGNVSRITII
                     RDELKEIEFTIKEVLSRQPRWILTSGGLGPTYDDITIESIGRALKRKFIYDPIALAIL
                     KRNYFKRGIKKLNKYQLKMSLVPENSQIIDNLIGTAPSVLIRHRETKIICLPGVPIEL
                     RQIFKTNILKMIIDDYKGIFFNEQFLKTIKIRESQIAKFLETIIEKYDEKEYDFYIKT
                     HAKNPTSIVPKMNIYVSIKGYDEKIVNIKFQNIINKIKKEIYRLEGEIL"
     gene            complement(14485..16902)
                     /locus_tag="VF222_02010"
     CDS             complement(14485..16902)
                     /locus_tag="VF222_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7141232.1"
                     /translation="MIKLTSFSIIIVTVFFFLVFNLSIEFSNQVESKNQNYSNVESVR
                     FIHYLDENVAIEEIKSGNLDTYFFRIPLDLVSDIKNDISINLFDKIGGSFGILFNPAP
                     SSNNDTMNPFEFRDIRFAINFLINRNFISNEVLHGYGIPMSDPFGLFSPDYPVVMDTV
                     ESFGFHYDPDYALKQIKKTLLAHGAVLKDERWYFKDKPIKLKVLIRSDDLQRKVLGEL
                     VSSELEQLGFTIIKDYSDLNKAVTLTQGSDPQKFLWHVYTEAFAGSTSFIKYNDAIVA
                     QMYAPWYANMPGNQNPGFWQYHNSTLDKITQKLVFSNFSSENERNNLLNEATRMGIEE
                     SVRIFLVKNIDPYAVSSSITGLVNDFGAGITSKYSLLNAKSERGDPLKVGVKQIYQGA
                     WNNIGGFSDAYSINIYSNIADSPTLRHPFTGEVISMRNQILNSTSTGPDERIPIHPDT
                     IIWNSKLGWVPVEKNSTSLSKVTYKILYSNWHNGIPMDKSDLLYSLYFMYEWGTNSGA
                     SDHTVDPEYTSRVELGLPYFKGIRFIPEHEDIVESYLDQWHYDNKEIAASAGVWAQEP
                     WEITAASERLVLSDDLSFSKSQSTTKSAEWLSLIIPEHANLIKKELINMKNENFIPPP
                     LKNIVSLKEAKNRYEASINWIEKHNHALISNGAFYLDRYNPSGRIIDIKLFNDSSYPF
                     ERDYWSNFENPKSIQVTSINFPKFIKIGEEKIFEFEILVDSVPITTTTSSSITAEFFL
                     TGYNGNIISHGKALPIHNKLGYFEIYLPKEETDKIPLGPVTLKLYASIDDSLRPFIST
                     NTFLAIH"
     gene            complement(16974..17888)
                     /locus_tag="VF222_02015"
     CDS             complement(16974..17888)
                     /locus_tag="VF222_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX7141233.1"
                     /translation="MVKDISLFGDRCPRCGKGPMVTDNSTGEMFCGNCGFVINERIEE
                     TGPEWRAFSKEEHEDRSRAGIPTSLAMHDMGLATIIGPVDKDASGKPLSSSMKSTIER
                     LRTWDSRSQVHEPVDRNFRQAFSELDRLKDKLAVGDAVIEKAAYIYRKALEKGLVRGR
                     SISALVAAALYAACRDTETPRTLKDIANASNIKKKDVARCYRLLIRELDLRMPVVDPV
                     KCVARIASKAGLSEKTKRKALEILKKAEEGKISAGKDPMGLAAAALYVACVMNGENKT
                     QKDVAEAAGVTEVTIRNRYKGLKTHLKL"
     gene            complement(18045..18353)
                     /gene="yciH"
                     /locus_tag="VF222_02020"
     CDS             complement(18045..18353)
                     /gene="yciH"
                     /locus_tag="VF222_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEX7141234.1"
                     /translation="MAVICKKCGLPDDLCPCGELEKEETRIIIRLETRRFSKTTTMIE
                     GIDPKVTNMHNIVKELKSQFACGGTAKDGFIMLQGDHREDVKSYLVKKGFNQEAIETQ
                     "
     gene            18484..18660
                     /locus_tag="VF222_02025"
     CDS             18484..18660
                     /locus_tag="VF222_02025"
                     /inference="COORDINATES: protein motif:HMM:NF002318.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HEX7141235.1"
                     /translation="MSSKKKNAPLPASSAGLLRFFEDETKGVKVKPEVVLGVTGALIA
                     ISIIIRVIFPVVGA"
     gene            18678..20495
                     /locus_tag="VF222_02030"
     CDS             18678..20495
                     /locus_tag="VF222_02030"
                     /inference="COORDINATES: protein motif:HMM:NF021370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HEX7141236.1"
                     /translation="MENGPDSVSNIHKRWSLTRINFSSYKISLGISIINSLILIIFFD
                     HFYLTLNLIQIVVFTITGISFLVFSYFLDLYLLRKTPVNKISKILHVSAFSNLLWLLI
                     LILGYLAFIIFQKEIPPKEYLLEGMMLAIGLRIGIFNSVFGANLLQAIKTAVIQPIVF
                     LFLIIPPSIVIDFFFDVAAISFGLILIGLGIVWTILADRAGRPNLQGTFALLQAFLSA
                     WTENKVENIEEILLAKSKDELVYTHIIKFINKQHHNLYWVLPNIHPGPFKEIGGSNLP
                     YQIYNHFSQKAVIFHSASDHSLNIPSKGEVIKYLQSLSNTKKRLAYGNKCSIPIQIKN
                     KKATATGIIFDNTPILILSFAPSGMEDIPEEISKELKAYSKNEGFKRLFIIDSHNAMG
                     KKIGKSENDDLLIAGKKCLNILKESPQYGFRIGISNTNEIKNHIVFGEDIGKSGLSII
                     LIDVNKDSNNNSNNNKKGNNDNQYVIGWADSNNMENGLREYVLQYMEQKGIRMLEICS
                     SDTHENSGFRTSEGYYPFGHITKFETIADHYYNLIELAYKKLELYVYEILTVESIVKV
                     MGKNQFMDYSNALDKAMDLTKKCLIITFGVILLMLIVTN"
     gene            complement(21025..21225)
                     /locus_tag="VF222_02035"
     CDS             complement(21025..21225)
                     /locus_tag="VF222_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141237.1"
                     /translation="MTIYLMSLVDYPQGRKAKITCANCNYVAKDERELDSHMKKRTYI
                     ITKLYLKPNLLSITSFIITHSI"
     gene            complement(21363..21596)
                     /locus_tag="VF222_02040"
     CDS             complement(21363..21596)
                     /locus_tag="VF222_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141238.1"
                     /translation="MTCKGICQRHKAIKPRNGGRYLIGQKRCQVCEIYLDWQGTWCPC
                     CGYRLRIRPRNGKYKLILRTVEYQKKTTEMNRT"
     gene            complement(21862..22215)
                     /locus_tag="VF222_02045"
     CDS             complement(21862..22215)
                     /locus_tag="VF222_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141239.1"
                     /translation="MATILTENMIEDISTTKDIVKEEQVNTCDICNSEITTIIYLNVS
                     SSSSSSIDYNNNSNSSKPKKSEVITNNDDYLSDKYYNVCLNCVEKITELMKYNYGFNG
                     RLIQFFESLTEKKLK"
     gene            22401..23090
                     /locus_tag="VF222_02050"
     CDS             22401..23090
                     /locus_tag="VF222_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX7141240.1"
                     /translation="MSHNAHIFNFYKRISEQYNDQGIMDYVKQLVPDTILKSYWDDQS
                     LKPLLHWFKIDFMKWMPNDLRCSTCNSLMRVQVIEGDSLILRKTEVYKCVKCGSTLIF
                     PRYVKILKIAETRVGRCSEWSILFGAILNSLSIQTRIVHDNLDHCWNESLINNKWIHV
                     DSTLSYPISFNHPYYYEQNWGKEYEYILAFSPNSIEDVTTCYTEKWQMVQQRRGKKDK
                     TDILKKIYSRL"
     gene            complement(24330..24467)
                     /locus_tag="VF222_02055"
     CDS             complement(24330..24467)
                     /locus_tag="VF222_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141241.1"
                     /translation="MKEWHFGTEDMFDNEDLECMTDDIMKIINKKCQLSQKQQARKKR
                     T"
     gene            25085..25228
                     /locus_tag="VF222_02060"
     CDS             25085..25228
                     /locus_tag="VF222_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141242.1"
                     /translation="MSRCLIPINEKLYLLLTMNFDQYNFDKIIMEKIIPLIKEKFVTN
                     KNE"
     gene            complement(25516..25902)
                     /locus_tag="VF222_02065"
     CDS             complement(25516..25902)
                     /locus_tag="VF222_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX7141243.1"
                     /translation="MAKFVEMDDRVKFKDQIEEKIMGSVILINKFNVAQGKVEQFLKD
                     WGEDATNFKQQPGFISTQLHKGIGKSSVFINYSVWESMEHYKKAINKILFRSKSQSPL
                     LKYDDESLVLSPHLFKKVAVPGICVD"
     gene            26100..26489
                     /locus_tag="VF222_02070"
     CDS             26100..26489
                     /locus_tag="VF222_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141244.1"
                     /translation="MTEKYGLPFEIVESKDIDKDKNKIKAGLKKIEIFASPEQVNEII
                     SVIESLNLEATLYDSHGYGQSKQKIRSGKAGGQTATISSSRKTIVTIAESDILEELVK
                     KLKHLNDNSEKKIGVISIQPVDTLVHL"
     gene            26830..26976
                     /locus_tag="VF222_02075"
     CDS             26830..26976
                     /locus_tag="VF222_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141245.1"
                     /translation="MSSQKKTNKERTVTPKEVLLLALHLKTVVKNVLLGIQDVDKSRI
                     SERK"
     gene            27089..27229
                     /locus_tag="VF222_02080"
     CDS             27089..27229
                     /locus_tag="VF222_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141246.1"
                     /translation="MIPTAIPQLDKEQTKQLVANIKKKTSKKDLLLWQNAIKQSKKIK
                     TK"
     gene            27583..27753
                     /locus_tag="VF222_02085"
     CDS             27583..27753
                     /locus_tag="VF222_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141247.1"
                     /translation="MRKALKYSERLDVGFGSNAIPSSVDFYKQNKFNLLKIQEKRREK
                     TMYQVIPKELFK"
     gene            complement(27940..28263)
                     /locus_tag="VF222_02090"
     CDS             complement(27940..28263)
                     /locus_tag="VF222_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141248.1"
                     /translation="MIKYYWQLRDVSNNNSNNNTRKKIDLVTKCCRSFINNLLKDLSN
                     KNRENEPNNFRLELLEQIYYVVDNYLMSVSLSKKPGKLTSNLLSSMEDVRFLTQLYAS
                     GCTES"
     gene            complement(28307..28591)
                     /gene="rnpB"
                     /locus_tag="VF222_02095"
     ncRNA           complement(28307..28591)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF222_02095"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(28613..29119)
                     /locus_tag="VF222_02100"
     CDS             complement(28613..29119)
                     /locus_tag="VF222_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HEX7141249.1"
                     /translation="MKVFVLELKQDDPTKCTAAKLVKFNLIQSSRFVPKDTIVLNPFS
                     KIALSKTDKSIAKSLCAIDCSWNCFDETIKKFKILKKRINRRLPLLLAGNPVNYAKLG
                     KLTTVEAIAGSLLILGFQSNAKELLNKFKWGHTFLDLNVELLELYKDQEDPNEILKIE
                     KDYFPNLY"
     gene            29257..30537
                     /locus_tag="VF222_02105"
     CDS             29257..30537
                     /locus_tag="VF222_02105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7141250.1"
                     /translation="MSEIMHLKFPFDLKKDQIDAVDEWMNKKGKGSIIYSTGTGKTEI
                     AFECARKMASLIREQNPLIVFKILFLVPRIVLIEQNIKRLIKYGIKKEKIGVYYGEKK
                     DIKEITFSTYQSIIKNFDLLRQSDMIILDEMHMVSETAKRFSKIFDVLHDNYDKLILG
                     LTATIDENDPRYSKIMNLIPPVKKYMLKEAVNDGRLSEPQIILKPVKLNFEERKIYEQ
                     TTTIIKDISLKLRASNPLIVSKLLKTGGSRARLAKLWFESVHKRKKLLNETNSKLNES
                     VNIIKNHPKEKIMVFSETIESINNISEILKKNKIPSEVIHNKVKTKQRQDILDSWGKN
                     FFVLLSVHTLEIGFDIPSVSIAIIASNTKNMHQLIQRIGRVIRKTEEKSRSLIYVVYV
                     DETKDKDILNLVRASLQSHSIKKPIKQDKISSYF"
     gene            30693..31937
                     /locus_tag="VF222_02110"
     CDS             30693..31937
                     /locus_tag="VF222_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141251.1"
                     /translation="MNLIASTRIMILAPQELENSASKSAAEAIRLDSQGSKLKAIQFY
                     QRAIEALNKLIQIYPEYKLNKIYLERSNAYLNRIKSLQMSSNVLEDEEGKCVEDIAEN
                     DTDNSERKIGSEMRKPKESLGEDFDSLIMKEKPNISWNEVIGLDDAKRAIRESIVYPT
                     KRSDLFPLGWPRGILLYGPPGCGKTLLAAASAAEIDGYFVNVDAAAMMSKWLGEAEKN
                     VSKLFKMANKLCESENIPILLFIDEIDSLIGTRNGEVGGEIRVKNQFLTEMDGINNKS
                     KISKLYVIGATNKPWSLEAGFLRRFQKRIYVTLPDNESRNYLFKQYTMPLKKDKTLKI
                     EELTKLVQGYSASDIKDICQAAQLRVVNELFESGKAMEAASNPRSISMNDFKEIIKIR
                     KPSVSVDMIRAYMRWSEQFKAL"
     gene            complement(31951..32481)
                     /gene="ppa"
                     /locus_tag="VF222_02115"
     CDS             complement(31951..32481)
                     /gene="ppa"
                     /locus_tag="VF222_02115"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7141252.1"
                     /translation="MNSNFIGKIASGIPNQINVVIEIPKGSNIKYEIDKDSGLLFVDR
                     KLFTAMFYPCNYGFIPSTLEDDGDPVDVLVIGEFVLVPLSIIKAIPIGVLLTEDEEGQ
                     DSKIIAIPLNKIDPTFSSITDVHNIPNTLKKQIEHFFAHYKELEGGKYVRITGWGNKE
                     MAETKITEAIERYLKT"
     gene            complement(32567..32869)
                     /locus_tag="VF222_02120"
     CDS             complement(32567..32869)
                     /locus_tag="VF222_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7141253.1"
                     /translation="MSTSEKKVSELRPGMRGVNVKVRIDSISEPRSVNLRTGGQAKVA
                     DAQVSDETGVIKLSLWDDQISMIEEGDMVVIENGYTHAYRGENNLNIGKYGKLQKI"
     gene            complement(33308..33481)
                     /locus_tag="VF222_02125"
     CDS             complement(33308..33481)
                     /locus_tag="VF222_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141254.1"
                     /translation="MKIICPDCKKTAHLEDDFSFVRCSYCSLDLTYGEYVKYVAYKDP
                     RYSDILSDYKNNR"
     gene            complement(33565..33729)
                     /locus_tag="VF222_02130"
     CDS             complement(33565..33729)
                     /locus_tag="VF222_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141255.1"
                     /translation="MESGVLGSVSYECMRCGTSVTIEELSALPEIKCICGFRVFRKAR
                     QPVVKQIKAI"
     gene            33861..>34054
                     /locus_tag="VF222_02135"
     CDS             33861..>34054
                     /locus_tag="VF222_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141256.1"
                     /translation="MAIMFLGTIAGFLNFAPKGNNNSQDPLDPEMFLNKLLSPSAANA
                     SALGNEQANITIVEFADYQCP"
BASE COUNT        11920 a         5197 c         5036 g        11901 t
ORIGIN      
        1 ctctcatcgc cgccgccatc gccgccgcca tcgccgccgc catcgccgcc gccatcgccg
       61 ccgccatcgc cgccgccatc ctcagctgta gcataaacag gttggatagt agaaatcgtt
      121 gaaaatagtc caacacgttc atcaaaaagg gagctgttct ggaatggaga ggctgtagtt
      181 aaaacaaagc taaaatatga aattatagct ataccaatca gtaacttttg tctcataaaa
      241 ttgattataa aatgttatgt aactataaaa ggttttatat ttataacaaa atcttcaata
      301 acataatgtt tgttttaaga aaaattcaaa gattcttaaa ataccaatac ttttactaat
      361 tagacgcaag acgcagtaaa acccatttac tacctccatc agtaaactag gtgaaccaaa
      421 ttgatccttt gaattcaaat ataattgcta taactaattt tattaatttt gtttatttga
      481 gagacattta tctcgaaaat tttccctatt cctggtatag atttaagaat atgaaataat
      541 taattgagat tttagtttat gtcattgaaa gtaacgtcta tactttatct caacatttct
      601 caattatcag atgtcttatt taggtgaaaa atctggaaaa aattcgaaga ttaaaggtaa
      661 tcagagaaag aaaaaccaaa tcaaacaaca acaatatgag atttaaacgt ttgattttta
      721 taaagaatcg agggaacttt aacatgctct ttgcaagata gggaaaacaa aggcttgaaa
      781 ataattaagt cttacaaacc aattaaaggc gtacaaaatc ccactttaac gatattcaag
      841 aatagttttg gtcgacttca acaacaatat taatatattc tacgaattca caaatctggt
      901 atattggata tatctataat tgcgttattt aataaaaaaa caataataat caataataca
      961 aattgctatt tatattctta cttttagtaa catattatat attgtttatt tttagaagag
     1021 attcaacaac attataaata tattaaaatt aactttattt gttaccacta ttgcttctac
     1081 taattatgtt caatttgtca agaacttcta aatatttaga aattagatcc agtttggtat
     1141 cttgaattag tatatcatta tcattctcta aagttgatat caaaaagttg attttatctg
     1201 tgagtatttt tgtatatgat atattggtta tgtgagcgtc aaagatggtt ggaacttttg
     1261 attcctttga taaattagaa tctggaatgg aatgttgttg ttgttgttct tgttgttgtt
     1321 gctcttcttc catttgttgt aaatcattac tcatctcttt tatttttctt ccacaactaa
     1381 cacatacagt ctcctcttta taacgaacct gaacgcctcc acatttagca caagaactgc
     1441 taagtaaagt acctcctttt aataacagat cagctgcact tttaatatta ttcctaccct
     1501 gatgattatt ctccttcttt tctttttcat ctgcagattt accattagac atagtattac
     1561 tattgatata tagaaagagg ggagtaattt atttatcttt tttttatgta attgttttgt
     1621 taattattta aattccatat tttagtttac taaaaagaca cataacgtag taaaaagtat
     1681 aattagtata ttatattggt agccccaaat ccttccctta ttgttctatt tgtactcata
     1741 ttataattat aaatttttaa tgaatattct tgtaaaacag gtagcatatc atttatagtt
     1801 ttacttagat aaaaaccagg acaatcacca gtaaattgaa aagttgcatg aactaatggc
     1861 ctgtttttat atgattccat ccatgatgca aaaggataca accagcatac acctggtttt
     1921 ccttcctgaa tttggcaact cccttggtca tctaaaaatc tacattttat aggcgttcca
     1981 tcctcttcat ctgtctcgtt tacctttctc tttaatgaga tcatagtgag atgagtgatt
     2041 aaatttccat aagagcccgg ttcattccac gaggatactt ttgtttcttt atccagaaat
     2101 tcattgagag atttataatt caatttattt ttcagagtat aaaggtcatc tttagttaaa
     2161 ggcaatcgtc cctgtttttc acagcagtta tgacaatcag gccacagaca gttccagaga
     2221 acaaatagac catcatttga tagttttggt atatgaaaaa ctacctggtt tactttaact
     2281 gtggtatcta ccacatcatt tcttctgcca agatgaagat catagatagg tttttcaata
     2341 ttccattttt ttgagagctc gtcaatagat gattttattg ttgttgtttc ctgactctga
     2401 tcgtgatgat gctcctgctg catattttac tataggaaat aaagaccttt aagtttaatc
     2461 attagtgcta ctctgctgtt gttaaagaaa agtctgctct aaagagtata tatttagtta
     2521 gtaataagta aaggaaacaa atcccataca gaaacaaaca aacaaaccat cagaattata
     2581 acaattttat tgcttctgga accagcaaat attgacactg aatattaaga atcttattta
     2641 ctgatgatgc caaattctgt actttcggtt tttctccatg atttattatc actcttttca
     2701 gttttggttt taatctacta atataggcaa gaagttgatt atagtcacta tggttgctaa
     2761 atccatgaat agaatgaatt ttgcatttta actttaactg ttgcccattt attgttactt
     2821 ccgtagtatt tcctgattga atttccttac cgagtgttcc tgttccttga tttgaaagaa
     2881 atattatttt actaagggga tcattgtgaa tttgctttaa gaattttaca gacagaccac
     2941 ctattagcat agatgatgga acaagtatta ttcctggttt ctctacttta gattttgaat
     3001 tgagtaattt aatattttta taattaaaaa ttgaaatatt ttcatttaaa atttttgttt
     3061 taacttcttt tgacagatag tcataatata attcatgcat atatgttgct tcaagtatgg
     3121 acttatccac gaagatttcc gctgatggat ccaatgaacc accttcctga tattttgtta
     3181 aagtatagat aatctcttga gaaagaccca taatgggaac tggaatgaga actcttcctc
     3241 catctctaag agtttgattg atgctttcta tcaaaattgg atcagtttct tcacgtttat
     3301 gaggtaaatc tttacttcca aaagtacctt caataataag cgtttcaacc cgaggaaaat
     3361 tccaagatgc attctcgagt aaattagtct ttccaaactt gagatcccct gtataaacta
     3421 aattatgatt tccatttcca atatgtaaat gaatagatga agatcctaat atgtgtcctg
     3481 aattacctaa tacaagtttt acatctggtg aaatatctgt aactattccc aaagataaag
     3541 gcatagtatg aattattaca ttttttatat cttcttcaga ataaatctca tggtttttat
     3601 gataatcaag gaaaagaaga atcattaaag gtaatgttgg ttcagtacaa taaatagggc
     3661 ctctataccc gtacttgaat agaaagggaa gaaaacccgt atgatcgata tgagcatgag
     3721 ttattataac tgcatcgatt tcatctaaag tgaaacctgt catatcaaat cgtgggaaat
     3781 gatttattgc tttatcctca tgaatattta ttccacaatc aagtaatatt ttactttctt
     3841 tagtcacaag taaaatacat gatctgccaa tttcaccaaa tcctcccaat gtcaaaatac
     3901 ttgcttcaca actattactt aattttggtc tgaaaacttt gtctccaact tctttataga
     3961 attttatcct atagtcagta gaattatcta aaactttatc aataaattga attgtagaaa
     4021 gttttactgt agattttctt atcttaattt tccagccagt ttcagtgact aaatccaata
     4081 atatagtatt ttcaatttga tttttcgttt caaatgaatt attgatatat attgttaatt
     4141 cacctaatgc atcatcaaaa ataattccat taatttcaac ctttaaaggc agtagacgag
     4201 tctgaataat attggtagaa ttgtttcgtg atttacgaat cgcttcatcg gttcttatta
     4261 ctactctctt tctaatcata tttaccatat tagaaaggat ttgggaattt tctatcaaaa
     4321 atctaggatt tttactatat agtgcgatac taggaccttc atattctatc tttgtcaagc
     4381 ccgattcctt tggcaagctc ttcagtatag tggccatgat gttctgactt gactgttcat
     4441 tatcaaaatt agtaaattcc atctctagtt ccttcagtat aatataatat acctcagttt
     4501 tctgaatgaa atataaattg attgcggtca aataatagaa aagtcatttt cttttttaga
     4561 taagattgaa atagtaatat ataataaatt caatgtaaag aaaggttaga tagtatggat
     4621 gtagaaagca acggggaaaa tactcgtaat atacccactg aaaataattc aggtaacgaa
     4681 acaggtaatt caggtagcga tacaggtttc cgaaagttta gatcttttag gattccagtt
     4741 aaagtaggag aagagtacga tgtcaaaatt gaagcaatga gtaaaagagg agatgctggt
     4801 gttgcaaaaa ttgagggaat ggtaatattt gtagctaaca ccaaggaggg agatgaagtc
     4861 aaaataaaaa tcacaagagt tggaatgggt tatgctatag cagatgttgt tcaacaatga
     4921 caacagcaac tagtttgata agaaatttgt ctaagatata ataattttca gaaatatatt
     4981 tattttattt ataattaaaa tttattttga agaaagaaag aaagaaagat atttgaaatc
     5041 aaatagtcat ttgatggagc ttccaaggaa aatacttgta ggagacaatg taatttcaga
     5101 ccttgggaat tttataactg atatcaatag tgaaatatcc aacgtcgtta taattagtgg
     5161 tgaaactgta aggaataaaa ttcaagataa tattaaaaga tcattagaga agtatcgtat
     5221 taaaaattac tggtttatta gaaaagaagc ttcttttgaa acagtatcta atatcaaaat
     5281 agagatcgaa gaaatagaac ctgatataat acttggaatt ggagggggaa aatcagttga
     5341 tgtgggaaaa atgattgcat attctataaa aaaaccgttt ataagtatcc ctacatctgc
     5401 ttctcatgat ggtatttcaa gtccatttgt atctctgaaa ggatcaaata aacctcattc
     5461 tattaaagta aacacaccaa taggaatact ggcagatatt aaattaatct ctgaagcacc
     5521 ttctaggtta ttgtcaagtg gttgtgggga tttaataggt aaactaactg cggttaaaga
     5581 ttgggaatta gcaagagatg ataaaaatga atattttgga gcgtattctg catatctagc
     5641 aaaattaagt gcggatatta ttatgaataa atcaaaagaa ttattattaa atgaatatgg
     5701 cgttagaact ataattgagg cgttaataag tgcaggtgtt gctgcaggaa tagctggaag
     5761 tagcagaccc tgttctggat ccgaacatct tttcagtcat gctcttgaat acattactga
     5821 tggtcattgt ggtcttcatg gagaaagggt aggacttgga acgatactca tgtccaaact
     5881 ttatggtatg gatttcgagg gcattatagc tgttctagaa aatgttcaag ctccagtcaa
     5941 agctgatcaa ataaaattaa ctgaaaaaga tgttgttcaa tcattgttaa ttgctcaaga
     6001 tttgaggcct gaaagatata ctatattaag taaaacaaaa cttgataaaa aatcagcata
     6061 tgaattagca aaatcagcaa aggtcattta agaaattaaa gttaagtgta ttgtaatata
     6121 caaactattt cttttatttt actaatgaaa ggtaataaaa ataattctta aagaaggaga
     6181 tttattaaaa tagcatccaa aatggtaatt actattttaa tttttatgat tatttgtttt
     6241 cagatgtgga tacactttca gatcttagtg aagtatcgga tctcggagaa gtattagatg
     6301 tttcagtttc atttgctgaa gcagatgttt cagtttcatt tgctgtagag gtctgtggtg
     6361 aagactcttt tgttttttgt gataggtata cttcctgaaa cgttacttta tttagaccat
     6421 ctacaaattt gaaaatatct gatgtgactg cttttttgat tatttgcaat ccatccaata
     6481 gagaaatatc ttcagataat tcaattatta ctgtgtcacc cttaaattcg aatttggcct
     6541 tttcatcatc tattggaagt cgtcttttta acaaatattt gattttatca tcatttgatt
     6601 caaccttttt aactacattt acatcatata ctagtactct acttgcaagt ctatgattat
     6661 aatcaatttg tacacggcca gaacctatat agcggataat accggttcga tcatccaact
     6721 ctacaacatc gcccaccttt atttcgttag ccttttcacc taattttctt tggggaatca
     6781 ttctgacttt atttgtatcc ctttcaccaa accctttgtc tggagaaatt tcaattgaaa
     6841 gtttttgacc tacatcaata ctagtaagag cttcatccaa accctttaat acccagcctt
     6901 ctcccacgga aataagtctt ggttcatatg ttctagttgg atcgtgtaga ttggattttt
     6961 ttgcttcgtc ttcaatagtt gtttcaaaaa tttcaccatt atctttgatt cgtgcagtat
     7021 aatctaataa tattaaagat cctttatcaa aaggcataaa aatcgctatt tatttaccct
     7081 atattaagtt tgacaaataa taaaatggac ctaaattata agtttttcaa tttttcttct
     7141 gccacagaca atgctttgtc gtttgcattt atcccaagca tcttcatagc cgagtaaata
     7201 gctgagacag ttcgcatgac atgatatcga gttacctcac ccatactacc tattctaaag
     7261 accttaccct tgaggtttcc aaatcctcca gctattagga ctctaaactc gtcggataac
     7321 aaagttctaa atctcttgtc atccattccc tccaggtagt tgaatgcgat aataacatta
     7381 ctcttagcat cttctttcgc aaagggagtg aggcccaatg cacttaaact agcataaaat
     7441 gcattagcac attgtctgtg tctttggatt cttttatcta ttccttcttc taggacaaga
     7501 ttcaatgctt catcaaatgc ataatacaaa ggtaatgcag gagtgaatgg ggtttcagta
     7561 tgctctttat aatatttgaa atatctattt atattcagat acaatatcgg tgctggattt
     7621 gattgcatgt atttttttgt tctttcattt attgatattg gagaaacccc tggtggagca
     7681 gctagggctt tttgtgatgc tgtaatacaa atatcaacat tccatttatc tactggaagt
     7741 tcatctcctc ccagtataga aacagcatct gcgataaaga atgagccata ttttccacaa
     7801 aggtcaccta atttatccat atatctaatt gtagtccctg tggaagtttc gttataaact
     7861 gcatacaaag ctttaacatc tttattaact tcaaatgcct cctcaatttt ttcaaatgga
     7921 ggattctgac cataaggagc atcaatccta attgcagtac cgccccaact gtcgattaaa
     7981 tctgcaagtc tgttactaaa ttcaccattt accggaataa ttgccttatc tcctttttta
     8041 atcaaattaa ctactgaaga ttctacagct ccagttcctg aagtagtaaa taaaataata
     8101 tcaccatttg tttcaaaaac tttttgagtt ttttcaatta tacctttgta taaaattctg
     8161 aaatcttcgc tacgatgatt gataacagga gcaagcatag cattcattac acgatttgga
     8221 acgttagtag gaccaggaag catcactaaa tattccatat ttccacatag attcagtagt
     8281 accgttattt aaaattactt aaatagcata atgtacgatt taattacttt aggtaggaaa
     8341 attgaatacc atgtttacaa ctagaaaaag gaaattatta gagaatgtat ctaaaatcaa
     8401 ttgtaaaaat atactttctt ttcaacctga gaatatattt tatcttactg gtttttgggg
     8461 agaaggagtt gtattgatga atcataatac tacaaagtta ttcacaccaa aattagagta
     8521 ttttcgagca tcagaagatt caaaagaatg tgaaattata aaaacagaaa ggggaagcga
     8581 taatatattg ttatccacca tttcagaatt ggacaataat ggtttttatt gtacagataa
     8641 tgccaattat aatttaataa atcaactgaa agaaaaggct ggatttaata aaataatagt
     8701 tgatgagggt ccatttttta aaactcgaag catcaaagac gaaatggaga taaaatacat
     8761 aaaacaagca gctaatatca tagataaatt gtatgatatc tgcttagaag aaataaagga
     8821 aggagtatca gaaagacaat tacaagcaat tttagtatat gaaacattca agaatggtgg
     8881 tggatttcct gcatccaaat ctacactaaa cccatttata atagcaagtg gaccaaattc
     8941 agcactacct catgcaagca tttctgatag aaaatttaaa agaggagact ttatcgttgt
     9001 tgatctcact ttaagagtta atggctatgt agcagatgct acacgaacat ttggtttgaa
     9061 aacaataaat ccggaattga aaaagatata cgaaatagta aaagatgctc aggtgttagg
     9121 attaaccaaa tgtagtaaca aaataaaaat aggagaagtt gacaaaacat gcagagaata
     9181 catttctaaa aatggatttg aaactgaatt taatcattct acgggtcatg gtataggatt
     9241 agatgttcat gagccaccct ggatccgccc taacaatgaa gaactactta tggataatat
     9301 gactattaca atagaacctg gaatatatct ggaatcaaaa tttggagtaa gaattgaaga
     9361 tagtattgtt atagacagta aaagaaacag aacaggaata ataaacctaa atacttttga
     9421 gaagaattta attattattt agatataatc aaataaaaga aaaaatattt ttttgtataa
     9481 tccaggcgaa atttgttaat ataacaatct tcgataataa taataattat tattattatt
     9541 attattattt tttctttgcg ataataattt caaataattt ctttgcaatt gaatccttat
     9601 tttgaacctt caaatgaact actggctctt tttgtgtaac aataaaaact tcattataat
     9661 tagatcctat tgtagtcttt attctcccta catcatttgc gactataaga tctgcattac
     9721 attctttcaa ttttttcttt gccttagtaa ttaaataatt atttgataca tcaaaatatg
     9781 ctttaaaacc aacaagaaat atatttttgt ctattaattt aatctcatca ataatttttt
     9841 tggtaggtac aagttctaaa ttaattggac gagaaatttg actaggaatt ttctttttat
     9901 aaatttgagg gagtttaaaa tctgttactg ctgcagccaa aataactaga tcaaatttct
     9961 ttgatctaat cctagaaata acttgtgtat acatttcatc agatgtatta accttaatag
    10021 tttctaaatt agatgaatta gacgaacaca gaacagaaga atgtcctaca acatgagtaa
    10081 cacgagcacc aagttcaagt gattctttaa ttatggcatt tcccattttt ccagaactga
    10141 gattagttat aactctaact gggtcaatat attccattgt acctcctgat gttactaata
    10201 tgttcttttc aaaaagatct ttttttttac ttttagtaaa aaattctaaa actgatttta
    10261 atgcttgatc tggttgaaga attttagctt ttccttcggt aatttcagga tctagaaaaa
    10321 ttacaccaag tttttttaat ttaagtatat tttcaacgac caattggttg gagtacattg
    10381 cttcatgcat agcaggtgca ataaagatag gaatttttga tcctaatcct acagacaaaa
    10441 tagaggttat tgtagtatcg tctattccat ttgcaaattt accaatagta ttagctgtac
    10501 atggatacaa aattattaaa tcggatttgt taaaatctgc taattcaata tgttccatat
    10561 ttgcggttaa cttgtcaaca acatcatttc cagtagccca tcttaaataa ttgggatgta
    10621 ataacatggt agctgacctt gaaattacgg ggaacacatt cgcgccatgc ctcattagaa
    10681 ggcgtgcaaa atcaattgct ttgtaggcag caacacttcc acatacacaa agaactatct
    10741 ttttgtcttt taaagtagtc cccaatgtat ttgttatatc cttggaagga tgtaaatcac
    10801 tagtcatttt ttttcacagg ttatgataat ttttttctga atcttttaat tgtttttaat
    10861 aaccgttcat attctttgtc ttcaattttg tatgaatgtt tatcctctgg aaatttacct
    10921 gtttttacat ccgtaatata attatgtaat gcattcagaa tagtctctga aaggttagtg
    10981 taagttttta caaatcgtgg tttgatatta tcatacagat ttaacatatc atgtaaaact
    11041 agaacttgtc catcacagtg agggcctgat ccaataccaa ttgttgggat ttttaaactt
    11101 ttagagataa attctgcaac ttcggacgta accatctcca atacaattgc aaaaactccc
    11161 gctttctcta gttctattgc ttctcttaat agttgagagc cctcaagagc agttttgcct
    11221 tgtactttat atccctccca gagtttagta gtttgaggtt ttaatcctat atgacccatt
    11281 acaggaattc ccaccttaat aattgccttg attctatcaa taatttcaat tcccccttca
    11341 agtttaactg cgtcacttcc tgattttaaa aatctaatag cattcttaac tgcatgattt
    11401 atatcaattt gataagagcc aaaaggcata tcagatacta taagagatct cttggtacct
    11461 acagatactg cttttgagaa aatgagcatt tctttcatat ttacaggtat agtagaatta
    11521 taccctagca taaccatccc tccactgtca ccaaccaata taatatcaat atctgtacta
    11581 tcacatattt ttgcactcca ataatcataa gatgtaatga cggaaatttt ttcccttttt
    11641 ttcatagata caatatcatt aacaaccaat ttctttagtc taggtttttg atgataatga
    11701 ttattgttat ttttactatt attattagta gtagtcatta ttgctcacat ttccatccac
    11761 gtattatttt taatgaatta tcaatattat atttattatt aaaattattc aatatttcta
    11821 atagagagtt ctgactcagt tgtttcaatt gttttgaagc tttaacgagt tctggaatag
    11881 atcttacaat gttgtctatt atacttattg tggcattctg tgccgttcgt gaaagaggat
    11941 tcagatcaac ggtgattact tttttgtgca tattaattaa cgcctgtgta cggtcaccat
    12001 cttctaatgg tacaaaaacc acatcagcca aaaatattcc ttcaggatca acatgtctcc
    12061 gattactagt tagctcaggt atttttaaaa attctttttg gcctactcca agaacaacat
    12121 tcgctccatt ttgttttaga gtttcggcga ttaaatattc tcttttttta tttctataaa
    12181 agagattaac ttctattgag gatccagttt cataggctaa atcaacaatc tctttagaac
    12241 acaatgctgc ggtatttccg tttacagata ttacaggtct ctgagcaagt aacaataaac
    12301 ttgatgctgc ttgaattgct tccaaagcgt acttagaagt tttttcccca ataagataat
    12361 cgaatgcttc tcctctacca tgagctatta gcccttcact aacgaccaga ccagatttga
    12421 acccattagt aagtaattgt cttatcgcaa gagattttgc tcttgggtga ttggatggta
    12481 tatgattaat atcaaacaaa atccactatc aaagctcctc gattatcaat atccgaaact
    12541 atcaaagtac cctgaaactt cttcaaaata gaggtaactt tagagatctc gttaggagga
    12601 accaatgtga aaatcgtttc cccaaacata cctatactac aatccactcc gaaatgattt
    12661 aattcttcta ttggttttgc acacaatcct tttgtcaatc ctaagaaatc ggcgaactca
    12721 cgagacatcc ttaaaaagga aggaacatct tttgataata ataattgttc taccattttt
    12781 ttgccaagag tattggcgtt atttgaataa ttagtaagaa tttgttttgt agaaagagga
    12841 ctaatacaga gaataatagc tttataatta tttaattcta ttttagttac tttaccgatt
    12901 ccaggagcgc caaaactagt cctgatctct agtccaccat aatattctga gattacagta
    12961 cctaaacccg tcttacattc aatttctgct atatgcgcga tttgagcggc ctgttccttt
    13021 aaaaaattta tgtcaagggc tttattcaaa gcataagaaa ggctcaaagc agctgcacca
    13081 ctggttccta atccataacc aatcggaata tctatagagt gatttatttt aatatagtag
    13141 gtaaaatcaa atttatcaag ataatattgt actatccatt ttgaaacttc tgcattattt
    13201 gttagtgaat tattaatgta gatctgatac cagttattct tagatttgta aagttctaca
    13261 gatgtggtta ctcctttttt aaaagaaaaa ccagcccctt tagatccata aaaaagtaca
    13321 tctgagtttt ggtttgtatt ctctggtttt tcgaagaagc cggtaatatg accaggactg
    13381 aatgctgtac aactagcaac tagctcaccg atttcagaag tggtcataga ctaaaagtaa
    13441 atataacatg aaaatataaa aatatcgata caaaccacta atgtaaaagt tagttttgta
    13501 aaagtagatt tttatatatt gcaattaaat tgtctgttat atggtaaact ttagtttata
    13561 gcaattttcc cattattctt gttatcctct ttaatcattt aaattaaata aataaatttt
    13621 taattaatct caatagaaag aatttttata ggaaaaatag aatatagtag gaggtaacta
    13681 ttgtccaagg atatagaaat tatttgtatt ggaaatgaaa tattgaatgg gactacaatt
    13741 gaaaaaaatt catgctggct agcaacaaga ataagacaga ttggaggtaa tgtatcaaga
    13801 ataactataa ttcgagatga attaaaggag attgaattta cgattaagga agtattaagc
    13861 cgtcagccta gatggatatt aacatcaggc ggtcttggtc ctacatatga tgatattact
    13921 attgaatcaa ttggaagagc gctaaaaagg aaattcatct atgatcctat tgcattagca
    13981 attctaaaga gaaattattt taagcgtggt ataaagaaat taaataaata tcaattaaaa
    14041 atgtctcttg taccagaaaa ttcacaaata atagataatt taatagggac agcaccatca
    14101 gtattaatta gacatagaga aacaaaaata atctgtttac caggtgtacc tatcgaatta
    14161 agacaaattt ttaaaacaaa tatattaaaa atgataatag atgattacaa gggtattttt
    14221 ttcaacgaac aatttcttaa gacaataaaa atcagagaga gtcagattgc aaaattttta
    14281 gaaacaatta ttgagaaata tgatgaaaag gaatatgatt tttatattaa aacacatgca
    14341 aaaaatccaa cttcaatagt accaaaaatg aatatttatg tttctattaa gggttatgat
    14401 gaaaaaatag taaatataaa atttcagaac ataataaata aaattaaaaa agaaatatac
    14461 agattagaag gagaaatttt gtaattaatg aatagcaaga aaagtatttg tagaaataaa
    14521 tggacgtaac gagtcatcta tactagcata tagttttaga gttacaggac ctaacggaat
    14581 tttatcggtt tcctcttttg gtaaataaat ttcaaagtat cccaatttat tatgtattgg
    14641 taatgctttt ccatgtgaaa taatatttcc attataacca gtcaggaaga attctgccgt
    14701 tatagaagat gatgttgttg tggttattgg aacactatca acaagaatct caaattcaaa
    14761 aattttttct tctcctatct ttataaactt cgggaaatta atagacgtaa cttgaatgga
    14821 tttagggttt tcaaagtttg accaataatc ccgttcgaaa ggataggaag aatcattaaa
    14881 taatttaata tctatgattc ttcctgatgg attatatcta tctaaataga atgcaccatt
    14941 acttatcagt gcatgattat gtttttctat ccaatttatg ctagcctcat atctattttt
    15001 tgcttctttt aggcttacga tatttttaag aggagggggt ataaaattct cattcttcat
    15061 attgataagc tcttttttta ttaaattagc atgttctgga attattaacg ataaccattc
    15121 tgcactcttt gtagttgatt gtgatttaga gaaagaaaga tcatctgata agactaatct
    15181 ttcagaagct gcagttatct cccaaggttc ttgtgcccat actccagcac ttgcagctat
    15241 ttctttatta tcataatgcc attgatctaa atatgattct acaatatctt cgtgttcagg
    15301 tataaatcta attcctttaa aataaggtaa tccaagctcc actctagatg tatattctgg
    15361 atctacagta tgatcactag ctccactatt tgttccccat tcatacataa aatataaaga
    15421 gtataataag tcagatttat ccataggaat tccgttatgc caattggagt atagaatttt
    15481 atatgtgact ttacttaatg atgttgaatt cttttccaca ggcacccatc ctaacttact
    15541 attccatatg atggtatcgg gatgaattgg tattcgctca tctggaccag tcgacgttga
    15601 atttaaaatt tgatttctca ttgaaataac ttctcctgta aatggatgtc taagagtagg
    15661 actatcagcg atattcgaat atatatttat gctataagcg tcactaaatc cacctatatt
    15721 gttccatgca ccttgataaa tttgtttaac acccactttt aaaggatctc ctctttctga
    15781 ttttgcattc agtaatgaat atttacttgt aataccagct ccaaaatcat ttactagacc
    15841 agttatagaa gaagaaacag cataaggatc gatattctta actaaaaaaa tgcgtacgga
    15901 ttcttcaatt cccattcggg ttgcctcgtt aagtaaattg tttcgttcat tttccgagct
    15961 aaagtttgaa aagaccaatt tttgagttat tttatctaat gtagaattat gatattgcca
    16021 gaatccagga ttttgatttc caggcatatt agcataccag ggggcataca tttgagcaac
    16081 aattgcatca ttatacttaa taaaagaggt agatccagca aatgcttctg tatatacgtg
    16141 ccataaaaat ttttgcggat ccgatccttg cgtaagggta acggctttat ttagatcact
    16201 ataatcctta ataattgtaa aacccaattg ttcgagttct gatgaaacta attctccaag
    16261 aactttcctt tgaagatcat cgcttcttat caaaactttt aatttaatag gtttgtcttt
    16321 aaaataccat ctctcatctt ttagtacagc tccatgtgcg agcaaagttt ttttaatttg
    16381 ttttaatgca taatctggat cgtagtggaa tccaaatgac tcaacagtat ccattacgac
    16441 aggataatca ggggagaaaa gaccaaaagg atcggacatt ggaatcccat aaccatgtaa
    16501 tacctcgttt gaaataaaat ttcggtttat taaaaaattt atagcaaatc taatatcacg
    16561 aaattcaaat ggattcatgg tatcattatt agatgatgga gcaggattaa acaaaattcc
    16621 aaatgaaccc ccaattttat caaagagatt aatactaata tcatttttta tatctgaaac
    16681 taagtcaaga ggaatacgaa aaaaatatgt gtccagattt ccagacttta tctcctcaat
    16741 agccacattt tcatccaaat agtggataaa ccttacactc tcaacattag aatagttttg
    16801 attttttgat tctacttgat tggaaaattc aatagataaa ttgaaaacca gaaagaaaaa
    16861 aacggtaaca ataataatag agaaactagt taatttaatc ataattaatg atcatcaaaa
    16921 atggttttat ctatacataa cgattattct ataaatacag atcaaaaaat tagttaaagt
    16981 ttgagatgag ttttcaatcc tttataacga ttcctaatag ttacttctgt aactcctgct
    17041 gcctctgcta catccttttg tgtcttattc tccccattca taacacacgc aacgtataat
    17101 gcagcagctg ctagacccat tggatctttc cctgctgaaa ttttaccttc ctctgctttt
    17161 ttaagaattt ccagggcttt acgttttgtt ttttcagaca atcctgcttt actagcaatt
    17221 cttgcaacac attttactgg atctacaact ggcattctta aatcaagctc tctaatcaaa
    17281 agtctataac aacgtgcaac atcctttttc ttaatattac ttgcatttgc tatatctttt
    17341 aatgttctag gtgtttctgt atcacgacat gcagcatata acgcggctgc aacgagagca
    17401 gaaatggatc ggcctctaac tagtcctttt tctaatgctt ttctatagat atatgctgcc
    17461 ttttctatta ctgcatctcc aactgctagt ttgtctttta atctatctaa ttcactaaaa
    17521 gcttgtctaa aattgcgatc gacaggttca tggacctggc ttctactatc ccatgttcga
    17581 agcctttcaa tagtgctttt cattgaagag gaaagtggtt taccagaagc atctttgtcc
    17641 acaggtccga taatggtagc taaccccata tcatgcatag ctaatgaagt aggaattcct
    17701 gcacgacttc tatcttcatg ttcttcttta gaaaatgctc tccattctgg tccagtttct
    17761 tcaattctct cattaattac aaaaccacaa ttcccacaaa acatctcccc agtagaattg
    17821 tcagtcacca tcggaccttt gccacatcgt ggacaccgat cgccaaaaag cgatatatct
    17881 ttaaccattc atttcaccac aaaacttttt aataactaaa tccgtactat ttatctttta
    17941 ttctaacata taaatattac cctaataata cgctggagtt tcaatattct attaaattta
    18001 ttctaaaaat atcaggaaat aatttctacg agggaccaaa taatctattg tgtttcaatt
    18061 gcctcttggt taaatccttt ttttactaaa tagcttttaa catcttcacg atggtcacct
    18121 tgtaacataa taaaaccgtc cttagcagtt ccaccacagg caaattggct tttaagctct
    18181 ttaacaatat tatgcatatt ggttacttta ggatctattc cttctatcat agttgtagtc
    18241 tttgaaaatc tccgagtttc aaggcggatt attattcttg tctcttcttt ttctagttca
    18301 ccgcaaggac ataaatcatc tggtaatcca cattttttac atataaccgc catattatat
    18361 ttatctatac ttttcttagc ttcttattaa gccttaccta ccaagttaga ttctaaattt
    18421 aatgaatgat atatttttac taaacgatag ctatttaagt aataaggttt tatgttttat
    18481 aaattgagca gtaagaagaa aaatgcacca ttaccagcct caagtgctgg tttattaaga
    18541 ttctttgaag atgaaactaa aggtgtaaaa gtaaaacctg aagttgttct tggagtaaca
    18601 ggagctttaa ttgcaatttc tatcatcatc agagtcatat ttccggtggt aggagcataa
    18661 ccactcacta gcataagatg gaaaatggcc ctgatagtgt atctaatatt cacaaaagat
    18721 ggtctctaac tagaattaac ttttcctcat ataaaatttc cttaggaata tcaataatta
    18781 actctttaat tttaataata ttttttgatc atttttatct aacactgaat ttaatacaaa
    18841 tagttgtttt tacaataaca ggaatatctt ttctagtatt cagttatttc ttagatctat
    18901 atttgttaag aaaaacaccc gttaataaaa tatcgaaaat tctgcatgtt tcagcttttt
    18961 cgaatctgtt atggttactc atcctgatct tgggatatct tgcttttatt atttttcaaa
    19021 aagaaatccc tccaaaggaa tacttgcttg agggaatgat gttagcgatt gggctaagaa
    19081 taggaatatt taattcagtt tttggggcta atttactaca agcgataaaa acagcagtaa
    19141 ttcagcctat agttttcctc tttttaatta ttcctccttc tatagttatt gatttttttt
    19201 ttgatgttgc ggcaatttct tttggtttaa ttttaatagg attaggtata gtatggacga
    19261 tacttgcaga cagagcagga agaccaaatt tacagggtac ttttgcactt cttcaggcct
    19321 tcctttcagc ttggacagaa aacaaggtag aaaatataga agaaattttg ttagccaagt
    19381 ctaaagatga attggtttat actcatatta taaaatttat taacaaacaa catcataacc
    19441 tttattgggt tttacccaat atacatcctg gtccttttaa agaaattggt ggaagtaatc
    19501 tcccttatca aatatataac catttttctc aaaaagcagt aatatttcac agtgcttctg
    19561 accattcctt gaacattcca tcaaagggcg aagtcataaa atacctacag tcattgtcta
    19621 atacaaaaaa gagattagct tatggaaata aatgttcaat acctattcaa ataaaaaata
    19681 aaaaggcaac ggctacaggg ataatctttg acaatacacc tatcctaata ctctcttttg
    19741 ccccttctgg tatggaagat attccagaag aaataagtaa agaattgaaa gcatattcaa
    19801 aaaatgaagg gtttaagaga ctttttataa tagacagtca taatgccatg ggaaaaaaaa
    19861 taggaaaaag tgaaaatgat gatttgttaa tagcaggaaa aaaatgttta aatattctaa
    19921 aagagtcccc gcagtatggt tttagaattg gcatatcaaa tacaaacgag attaaaaatc
    19981 atatagtctt tggagaagat attggaaaaa gtggtctctc tattatcctt attgacgtaa
    20041 acaaagattc taacaataac agtaataaca ataagaaggg taataacgat aatcaatatg
    20101 taataggttg ggcagattcg aataatatgg aaaatggatt gagagaatat gttctccaat
    20161 atatggaaca aaaaggaata agaatgttag aaatttgttc atctgatact catgagaatt
    20221 ctggatttag aacatccgag ggatattatc cttttgggca cataacaaaa ttcgaaacaa
    20281 tagcagatca ttattataat cttatagaac tagcgtataa aaaattagaa ttgtatgtat
    20341 atgaaatact aaccgttgaa tcaatagtaa aggttatggg gaagaaccaa tttatggatt
    20401 attctaatgc tttagataaa gcgatggatt taacaaaaaa atgcttaatc atcacctttg
    20461 gagtaattct acttatgtta atagttacaa attaaattga tatattcttc gttgtattat
    20521 ttactttaat gcttattgca ttagtttaat ttaagtatct atgtaattat agataataaa
    20581 aaaatacatg ttttatattt aacaaatatt aatttttagt taattataaa aaatattcaa
    20641 gtagtttttc ttataatgaa attggacttt aattgaaatt gttacaaaaa tttcaatact
    20701 gatgtaataa tctattattg cagatagttg tatttattta gattagatgt tttttacttt
    20761 cagacaatct caacaacata gttagagata aataatattt aatttagatt tctaaaatct
    20821 taaattaaaa taatgattct tttagatatc gataacaata tcacttaacc tttggaaaat
    20881 taatataatt ttttaacatt attataagtt aaaatcgtaa atactcctta atagataaag
    20941 ctagagaaca ataactttaa tatgttatag taatgcagca gattatgtgt ttgtcaaaat
    21001 aaatcatcta ttagaattga attattaaat agaatgagtt ataataaaac tagttataga
    21061 taacaaatta ggctttagat ataattttgt tattatgtat gttctttttt tcatatggct
    21121 gtctagttct ctttcatctt tagctacata attacagttt gcacaagtta tcttagcttt
    21181 tcgtccttgt gggtagtcga ccaagctcat tagatatatt gtcatttctc ccgccccaca
    21241 taaagtataa acagccaaat tttatttata acttttagag taattattat ttgtgtaact
    21301 ataatctatt ttcttttatc attaataatt gtgttatttt taattaatat atataaatta
    21361 aattaagttc tattcatctc agtggttttc ttttggtatt caactgttct taatattaat
    21421 ttatattttc catttcttgg tctgattctt aatctataac cacagcacgg acaccaagta
    21481 ccttgccaat caagatagat ctcacaaact tgacagcgtt tttgccctat caaatatcgt
    21541 cccccgtttc gaggttttat tgctttatga cgttgacaaa ttcccttaca tgtcattttt
    21601 catcagtaaa ctaagtgaaa acataataat aactataatg taattttgag gtattatata
    21661 ccatgacatt tccttaggta aattcgtata ttaaattata tcttatttgt gattttatat
    21721 tatgatggtt ttttcgattc tatttcataa tcaagaaaaa actctcatgc acagataact
    21781 gaaaatatct gctatcacaa tcataatttt caatactaca tacaacaaaa aataaaataa
    21841 gaatattttt gattttaatt tctattttaa tttcttctct gttaatgatt cgaaaaattg
    21901 aattagtctt ccattaaagc cataattata tttcattaat tctgtaattt tctcaacaca
    21961 gttcaagcaa acattgtaat atttatcaga aagatagtcg tcattgttag tgataacttc
    22021 agattttttg ggtttgctac tgttgctgtt gttgttgtag tcgatgctac tgctgctact
    22081 actactaaca ttcagatata ttatcgtggt aatttcacta ttacaaatat cacaggtatt
    22141 gacttgctct tcttttacta tatcttttgt ggttgaaata tcttctatca tattttctgt
    22201 tagtattgtt gccaataatg ttttccttca ttaattcatt tttcacttct taaattattt
    22261 cacaatcctg gtaacctaag ttagttatca ataaaatctg caatttttgt ttttatattg
    22321 gaagaaacaa ttaattctat cgaaaccatt gaatatttgt cgtaaaaaag attatgtttg
    22381 ttaatagttt gaatcggatc atgtcccaca atgcacacat ttttaacttt tataagagaa
    22441 tttcagaaca gtataacgac cagggaatca tggattatgt caaacaacta gtaccggaca
    22501 caatattaaa atcatattgg gatgatcaat cattaaagcc tctcttacat tggttcaaaa
    22561 ttgactttat gaaatggatg ccaaatgatc tacgatgttc aacttgtaat tcactgatga
    22621 gagttcaagt tatagaaggc gattcattga tattgcgaaa aacagaagta tataaatgtg
    22681 ttaaatgtgg atccacacta atatttcccc gatatgtgaa aatactaaag attgcagaaa
    22741 cacgagtcgg tagatgcagt gaatggtcta tactgtttgg tgctattcta aattcattat
    22801 caattcaaac tagaatagta cacgataatc ttgatcactg ttggaatgaa tctttgataa
    22861 ataacaaatg gatacacgtt gactctactt tgagctatcc tatttctttt aaccatcctt
    22921 attattatga gcaaaattgg ggaaaggaat atgagtatat tttagcattc tctcccaata
    22981 gcatcgagga tgttactaca tgttatactg aaaagtggca aatggttcag caaagaagag
    23041 gaaagaaaga taaaacggat atattgaaaa aaatttactc gagattataa atttattaaa
    23101 tgacaaaata gatagatcaa tatcatgtaa actcgttaaa ctctaattat actaccatta
    23161 ggttatatat aacaaaatta ctgatatggg aagaaaagaa acagaaatga taactttaac
    23221 taattttcga tccgttacct gtgagatttg tgcctatctg acctttctct attacctctt
    23281 aactagatta gacacttaaa tctcacttcc aaagataatt tatatttaca ccaagtagaa
    23341 taaatcaatt aagaccagca agtatagtct actcttcaaa aatgattcca gtatatgttg
    23401 aggggggggg agagagagaa aaggagtaaa gcgaaagttt aatgattttg ttatatctat
    23461 tatgactaga agtaattagt cgattgaata aattatttat gttctgaata ctggcttctc
    23521 aacaatatca tcatcgtctt ttatagtgta gaatatatga ttataactcc attctatgtc
    23581 cttaggttca ttgcatttat caatgccttt gtgtaatcgg aatttctgca ggataagata
    23641 atgatgtttg gagtagtcca tcagccctta gatagtatat tatcatattc acaattattt
    23701 cgtattcagg acttccctga agatttacct attttttata tttctctaag ttgggctttt
    23761 atgcatccat tggaatcttt ccaaaaattt tacgctttaa tataaaattt gatgtattgc
    23821 tatttgaaac tttatttaaa caattaagga aaccaaggtg ctaaaattct tgtatcccgt
    23881 tcttgctaga atcattagag tttattttat aacaaaaagt taacagatat tatctaatta
    23941 tttatagatt ttttacatca ttatttgata taaacgataa atgtttgttt tatagatgaa
    24001 atattaaaat aatatgaata atgtagaaga aattatacct tttgttattt tcgttagttt
    24061 aatagtggaa gtcattttgc gaactataga ctatattatt gagaataaag aaggagttat
    24121 ttttaggtaa aggagaaaaa atctgaagat aataatgcta tgggtgttat taatcatccc
    24181 attaccccat ttactttcaa tgaaatcacg aatttgttgc tcggtcttaa gaataagtgg
    24241 taacattgac tttgttggag tagaagaatc ttctaataac tagattcttc aaagcgattc
    24301 gtttattctg gaccattgtt catatttgat tatgttcttt tttttcttgc ttgttgtttt
    24361 tgtgatagtt gacatttttt attaattatc ttcataatgt catccgtcat acattctaaa
    24421 tcctcattat caaacatgtc ttctgttcca aaatgccatt ctttcaatat gttgttgata
    24481 tctttcttag gtcagtagtt gttgatcttg ctgttgctat cgccgtcgat accatcatgt
    24541 tggtttaaac atcagtttaa tgtaattcaa taaacgtgtt ttccattcgt tactattatt
    24601 tttcttcatt tcatttattt tcatatcaat atagctgttg tcgatttata gattaatgct
    24661 ttaaactcta aataaattga taaacaaata aattaaaaca cgaaatactt tagagaattc
    24721 gattttactt cttttctagt taaattaact aactctttag taaaataact tttttattat
    24781 aatcgaaaat tacttattaa ctacacacac atgttggaag aaatgataat gatattgata
    24841 atactaaatt cttttatgct tttacccaaa gtatattgaa ttatgatggg tcactaagat
    24901 cgataggaat aactgttcaa atggtattat tataaatgaa cgatatagag aaggactaaa
    24961 accccttctt actatagaag aaaatcacga ttttgcaatt aatacaataa atagacctaa
    25021 aacaagacta aaatttgaac caaaaatggc tgaattaact tatacgttta ggagatataa
    25081 aaaattgagc aggtgtttaa ttccaatcaa tgagaaattg tatttacttt taactatgaa
    25141 ttttgatcaa tataattttg ataaaataat aatggaaaaa atcattcctt taattaaaga
    25201 gaaattcgtt actaacaaaa atgaataaga aaattcaggc tatatcaaat ataaattcca
    25261 tgataaagat tctgatctct acatattatt attgttaatc ggttcagatg aaattgaaaa
    25321 tgaatatttg gttattggaa ttaccgaagc agaaatagat aaagcaatat tctaaccata
    25381 tactttaaaa tggaattgtc cagaatctaa tataaaagat tgataatatt cataaataat
    25441 tcatcagtag ataataacaa gaaatgatta aagtaaaaat agaaaaaact tctatctata
    25501 tttatttcta ataatttaat ctacacatat tccaggaacg gcaaccttct tgaataaatg
    25561 gggggaaaga actaaactct catcatcata ttttaaaagt ggcgattgtg atttggacct
    25621 aaacaatatt ttattaatcg ctttcttata atgttccatt gattcccaaa ctgaataatt
    25681 aataaataca ctactttttc caatcccttt atgcaattgt gtagaaataa atcctggttg
    25741 ttgtttaaag ttggtagcat cttctcccca atcctttaaa aactgttcaa ccttaccttg
    25801 tgctacattg aatttgttta tcaaaattac tgaacccatt attttttctt caatctgatc
    25861 tttaaatttc actctatcat ccatttcaac gaattttgcc attataatat tataattttt
    25921 gacatattat aatataatta tctctccttc gaatagtagt aataatctag agttaatcgt
    25981 atattatgaa gttcctcaag gccagagaga caaagtaaaa caaaaaatga ttaccgaaaa
    26041 aaataatatt attctataaa caaaacttta tctatataat acataatata ttgtttctaa
    26101 tgacagaaaa atatggtttg ccgtttgaaa tagtcgaatc caaagacata gataaagata
    26161 aaaataaaat taaagcagga ttaaaaaaaa tagaaatatt tgctagtcct gaacaagtta
    26221 atgaaataat ctcagtaatc gagagtttaa atttagaagc aacattgtat gactctcatg
    26281 gttatggaca gtctaagcag aagataaggt cgggaaaagc cggaggacaa actgccacga
    26341 tatcatcgag tcgtaaaacc atagtaacta tagcagaatc tgacattcta gaagagttag
    26401 tcaaaaaact gaaacactta aatgataata gtgagaaaaa aattggagtt atctctattc
    26461 aaccagtcga taccttagta catctatagt ttattactac atcatatcat caacatatca
    26521 tcatgtcata ttttttttcc ttctttcttt tttctcatag tatagtataa atttcaagtt
    26581 catgcctcta aaaaggcaag atggaagaag ttaaacaaag atttagtact tattatttaa
    26641 aagtatctca ttagccagtc aaagattaat aaggaaaggg aaaaggaact taaatttata
    26701 tttttatatt agtgcattct agtaacatag aagaaaatga ataacgttgt aaaaacagga
    26761 ttacttacat ttgttttact gtaattgcaa tagggttatt tggaatctca acagtatcaa
    26821 tggtcaatga tgtcctcgca gaaaaagaca aacaaggaaa gaacagtaac accgaaggaa
    26881 gtactgctac tagcacttca tctaaaaacg gtagtgaaaa atgtacttct tgggatccaa
    26941 gatgttgata aatctagaat ttctgaacga aaataatgat tttttttatt cgtccttttt
    27001 taattgattt gtttgtttgt ttatacctag gtttaaaaga aaaataattt agagtttgtt
    27061 tgtttatatc taagtagagg atattaagat gatcccaaca gcaattccgc aactagataa
    27121 ggaacaaacc aaacaactgg tagcaaacat taaaaagaaa accagtaaaa aagatttgtt
    27181 attgtggcaa aatgccatta aacaatctaa aaaaataaaa acaaaataat attttaaata
    27241 tgcttattgt tggataaaag tccaaaatta actttaagaa atttaattgt tgatgaatcg
    27301 ataagtaata gtttgtattg tggagagtaa aaattaaaca actttattca taaagaagtt
    27361 tcttatcaga aagaaagact aggaagcact catcttattc atttggacga cctagtggga
    27421 tttataacca tctctattgg tgaagtatga actaagttaa tggattttaa tgaaacttta
    27481 aaaattagac ccaaaaaata tcctgcttta caaataggac aattagcaat tgataaaaaa
    27541 tatcaaagac aagcatatgg ctaaataatt atacaatggt gtatgagaaa agcattaaaa
    27601 tattctgaaa gattggacgt aggttttggt tctaatgcca taccttcatc agttgatttt
    27661 tacaaacaaa ataaattcaa cctattaaaa atacaagaga aaagaagaga gaaaactatg
    27721 tatcaagtaa taccaaaaga attattcaaa taagaatttg ttcctttgtt gctaaggcta
    27781 aagtttgaat tgttatattt atgatttcgt ttgtgtcatt tggcacatcc aaatctttat
    27841 aaactgcttc tttaactttt gtttatttct tattttctta ttttggagga tcaaactaat
    27901 tgatctgttt gatactgctt caagatcttt gtccttattt cagctttcag tacaaccact
    27961 agcatatagt tgagttagaa acctaacatc ttccatacta ctcaatagat tagatgtcaa
    28021 ttttcccggc tttttagata gagaaacgct cattagatag ttatcaacaa cataatagat
    28081 ttgttcaagt aattccaatc taaaattatt tggctcattt tcacggtttt tgtttgataa
    28141 atcctttagc aagttattga tgaaagatct gcaacatttt gttactaaat ctattttttt
    28201 tctagtattg ttgttgctgt tgttgttact aacatccctt aattgccaat aatattttat
    28261 caatctatat taataattat tgttagaaca cacatttgaa aggagtgtgt gctagcaata
    28321 accctccttc tgttttatac gagatttcgc ttagcggcca acctgagcca gtgcaggtac
    28381 atgttgctct ttttggcctt gctccactca agtcgtcagt ttcattctag gaaagacaaa
    28441 ctcaggttat tagccatcat agggttataa tcagtcctct agatactttt ctgttacgtt
    28501 gccagctgtc tccagctaat tatctctaat ttgagtgcct gctatgaggg aggagtttcc
    28561 tctctttcga gtcactcgtt cactagctca caaatttata atcaaatata acctaatata
    28621 ggtttggaaa ataatctttt tcgattttaa gaatttcatt agggtcttct tggtctttgt
    28681 ataattctaa tagctcaaca tttaaatcta aaaacgtatg accccatttg aatttattca
    28741 ataattcttt tgcatttgac tgaaaaccga gtataagtaa agaacctgct atagcttcaa
    28801 cagtggttaa ttttcctaat tttgcataat ttacaggatt tcctgctaga agtaatggaa
    28861 gacggcgatt aattcttttt ttaaggatct tgaatttttt tattgtttca tcaaaacaat
    28921 tccaagaaca atctatagca cagagtgatt tagcgatact cttgtcggtt tttgataatg
    28981 caattttaga gaatgggttt aaaactatag tatctttcgg tacaaatcga cttgattgaa
    29041 ttaaattaaa ctttaccaat ttagctgcag tacatttagt tggatcatct tgttttagtt
    29101 ctaaaacaaa aaccttcatt tacaatggtt attaaaatat gctattatct atttaaatgg
    29161 tcgaaatctt aatacaaata ttttgtatct tactaatata aaaaaatcaa taatacaacc
    29221 taaattatta ataacttctt tgttattgta atattcttgt ctgaaataat gcatctaaaa
    29281 tttccttttg atttaaaaaa ggatcagatc gatgcagttg atgaatggat gaataagaaa
    29341 ggcaaagggt ctattattta tagtactggt acaggaaaaa cggaaatagc ttttgaatgc
    29401 gcaagaaaaa tggcttctct aatccgagaa cagaatccac taatagtctt caaaattctt
    29461 tttttggttc cacgaattgt attgatagaa caaaatatca aaaggctgat aaaatacggt
    29521 ataaaaaaag aaaaaattgg agtttactat ggagaaaaaa aagatattaa ggaaattacc
    29581 tttagcactt atcagagcat tataaaaaat tttgatttgc taagacaatc agatatgatt
    29641 atacttgatg aaatgcacat ggttagtgaa actgccaaaa gattcagcaa aatttttgac
    29701 gtattgcatg ataattacga taaattaata ctaggtttaa cagcgacaat tgatgaaaat
    29761 gatccaagat attctaagat aatgaattta attcctccag taaaaaaata tatgctaaaa
    29821 gaagcggtta atgatggaag attaagtgaa ccacaaatta tattaaaacc agtgaaactg
    29881 aattttgaag aacgaaaaat atatgagcag acaactacca taattaaaga tatatcatta
    29941 aaacttagag catcaaatcc tttgatagta tcaaaattat taaaaacagg gggatcaaga
    30001 gccagattag caaagttatg gtttgaaagt gttcataaac gaaaaaaatt gctgaatgaa
    30061 actaactcaa aattaaatga atctgtgaat ataattaaaa atcatccaaa agaaaagatc
    30121 atggtattta gtgaaacaat agaatccata aataatataa gtgaaatttt aaagaaaaat
    30181 aaaattcctt ctgaagttat ccataataaa gtaaaaacaa aacaaagaca agacatttta
    30241 gattcttggg ggaaaaattt ttttgtattg ttatcagtac ataccttgga gataggattc
    30301 gatataccgt ctgtaagtat agccataata gcatcaaata cgaaaaatat gcatcaacta
    30361 atacaaagaa taggacgagt aataagaaaa acagaggaaa aaagtcggtc tctaatatat
    30421 gttgtatatg tagatgagac aaaagataaa gacatactta acttggttag agcatcatta
    30481 caaagccata gtataaaaaa accaattaaa caagataaga tctcatctta cttctagtca
    30541 attagagaaa tgcatttcag ggtatttctt ctttaatatt gattgaatgg taaagaaata
    30601 gaattctgaa tatataaagg ttaaaattta ttgtatatta tttttagtgt ttagaacaat
    30661 taagatttac atcatagtac taaaaatcaa ttatgaactt aatagcatcg acacgaatta
    30721 tgattttagc tccacaggaa ttagaaaata gtgcaagtaa atcggcggct gaagctataa
    30781 ggcttgattc acaaggttca aaattaaagg ctattcaatt ttatcaacga gctattgagg
    30841 ctttgaataa attaatccaa atttatccgg aatacaaatt aaataagata tatttagaaa
    30901 gatctaatgc ttatttaaac agaattaaat cgttacaaat gtcatcaaat gtattggaag
    30961 acgaagaagg aaagtgtgtt gaagatatag ctgagaatga tactgacaat tctgaaagaa
    31021 aaataggaag cgagatgagg aagccaaagg aatcacttgg tgaggatttt gatagtttaa
    31081 ttatgaaaga aaaacctaat atttcgtgga atgaagtaat tggtcttgac gatgctaaaa
    31141 gagcaataag ggaatctatt gtttatccta caaagagatc tgatttattc ccgttaggat
    31201 ggccaagagg aatactacta tatggaccac cagggtgtgg aaaaacatta cttgcagcag
    31261 cctcagccgc ggagattgac ggttattttg ttaatgttga tgcagctgca atgatgagta
    31321 aatggcttgg tgaagctgag aaaaatgttt caaaactttt taaaatggct aataagttat
    31381 gcgaaagtga aaatattccg atattgcttt ttatcgatga aatagattct ttaataggaa
    31441 ctaggaatgg cgaagtagga ggagaaatac gtgttaaaaa ccaattctta actgagatgg
    31501 atggaataaa taataaatcg aaaatttcaa aattatatgt tattggcgcg acgaataaac
    31561 cctggagctt ggaggcaggt tttcttagaa ggtttcagaa gagaatttac gttactttac
    31621 ctgataacga atcaagaaat tatttattta aacaatatac aatgccatta aaaaaagata
    31681 aaacactaaa aatagaggag ttaaccaaac ttgttcaggg gtatagtgct agtgatatta
    31741 aagacatctg tcaagccgca caattaaggg ttgttaatga attatttgaa agtggaaagg
    31801 caatggaagc agcgtctaat ccgcgttcca tatctatgaa tgattttaaa gaaattataa
    31861 aaataagaaa acctagtgtc agcgtagata tgattagagc ttatatgaga tggagtgaac
    31921 aattcaaagc attataatca aggtaaaaag tcaagttttc aaatatctct caatagcttc
    31981 agttattttt gtttccgcca tttctttgtt cccccatcct gtaattctta catattttcc
    32041 gccttctaat tccttataat gtgcaaaaaa atgttcaatt tgctttttta aagtatttgg
    32101 tatattatga acatccgtta tagaggaaaa agtagggtct attttattaa ggggtattgc
    32161 aattatttta gagtcttgtc cttcctcgtc ttcagttaat aacacaccta tcggaattgc
    32221 tttgattata gaaagaggaa ctaatacaaa ttcaccaata accaaaacat ccacaggatc
    32281 gccatcgtct tccaaagtag aagggatgaa accataattg catggataaa acattgcagt
    32341 aaacaatttt ctatctacaa ataacaaacc actgtctttg tcaatctcat atttgatgtt
    32401 acttcccttt ggtatttcta taacaacatt aatttgattt ggaataccac ttgctatttt
    32461 tcctataaaa ttggagttca catcaagatt atttaaaatc ccatttataa ctcaacatcc
    32521 cagttaataa actcaaaatg atattatgaa taatttagaa tatttgtcat attttttgta
    32581 atttcccgta tttaccaata ttgagattat tttctcctcg atatgcatgt gtatatccat
    32641 tttcaattac taccatgtct ccttcctcga tcatactaat ttggtcatcc catagagaca
    32701 acttgatcac gcccgtttca tctgaaactt gagcatcggc gacctttgcc tgtcctcctg
    32761 ttcttaagtt tactgatcgt ggttcagaaa tactatctat cctaactttt acatttactc
    32821 ctctcattcc aggtcttaat tcacttactt ttttttcact cgtactcatt actaattaat
    32881 aagataaaga agaataataa aaatgataat atttttttaa tattatattt gtttataatg
    32941 actattgaga gctccaactc atgaataaat acgtaaaaaa ataactattg atatctaaca
    33001 attacaattg acaaagaaaa tacaggatat gaacaagggc aataaccaac cactaaccaa
    33061 tggtcaagcc ttgtaaatat ccattgatat tgattttttg ttatttttta taaatatttt
    33121 aagaaacaat tcaaatcgtt ttgtttttat aaattaacgc taacatagaa atctttttta
    33181 ttatagcgtt aattgtatat gtaaacgcag gttaatatta aaatatcata attataattg
    33241 cacaaatata actctgatga aaagtaataa aaaactttga ttcgaaaatg aaaactttag
    33301 aatagcatca cctattgttt ttataatcac tcaatatatc actatatcgt gggtctttat
    33361 atgccacata tttaacatat tcaccataag taagatccaa tgaacaataa ctgcatctta
    33421 caaaggaaaa atcatcttca agatgggctg tctttttaca atcaggacaa attattttca
    33481 cactataaaa tagcgattaa gatataaata acttggtatt ttattatctt accacataag
    33541 gaaatacctt tagaaagagt attattagat cgctttaatc tgttttacaa ccggctgtct
    33601 tgcttttcta aaaactcgga acccacaaat acattttatt tcaggcaatg cagacaattc
    33661 ttctattgtt acactagttc cacatctcat acactcataa gaaacactac ccaaaactcc
    33721 agattccata acttttaacg tatttgagat taatttaatt gtactgaaaa atggaaatca
    33781 agttccacat gattaataag tttgttaata acaatatcta acaaatgaga aaaaaaattg
    33841 taattgcaag tgtttcgtta atggcgatta tgttcctagg tactatagct ggttttttaa
    33901 attttgctcc taaaggtaat aataatagtc aagatcctct agatcctgag atgtttttaa
    33961 ataaattgtt aagtccatct gccgctaatg cctctgcact aggaaatgaa caagctaata
    34021 taacaatagt tgagtttgct gattatcagt gtcc
//
LOCUS       DASVEV010000040         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_10352, whole genome shotgun sequence.
ACCESSION   DASVEV010000040 DASVEV010000000
VERSION     DASVEV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10352"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VF222_02140"
     CDS             <1..461
                     /locus_tag="VF222_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX7141257.1"
                     /translation="LWPVILSYGTSFIILGFFWIAHDYQFHYVKRANRTFLWITIFYL
                     MFIAFVPFSTSLIGEYGDQQISVIIYAVNISIIGFWEYIRWRYATKDHHLVDSDLDPT
                     FITIMSRRFLLGPTIYLIAVAISFISTQVSLVLFIATPLYFLVSAKKDKS"
     gene            1269..2582
                     /locus_tag="VF222_02145"
     CDS             1269..2582
                     /locus_tag="VF222_02145"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7141258.1"
                     /translation="MNRDNISALVVAIMVLYVIFSFTIYFNNPGLNKVWPIQQKDKDM
                     LVLGLHSPEFTFEKNYTNVKTAVQRFGITNPAVADTTIENHTVTMKINKTQFQQVNES
                     QFKKAPEFAQISGYINTPNNNPLTLSSLKGKVVLVYIWTYTCINSIRPMPYIDDWNQK
                     YSNKGLVIVGLHSPEFTFEKNYTNVKAAVQRFGISYPVILDSDHGTWDAYGNQYWPRF
                     YLIDTQGNIRYDHIGEGSYDQIEKAIQLLLAERAALMGAKEISFNTKPTTVIKPGSLY
                     YVDLRQSTTPEIYIGYSTARTPLGNPEGFKPDQTVSYSIPSNTNFKLHIAYLQGNWKN
                     NPDNMQLQSDTGRIVLTYYAKSVNIIAGGKGGGAVFNDEGIEGTTAAASTSNKSLGED
                     LSSDGSFRIDGQRLYNLAIHNNYAVHNIVIDVKGKGFQFYTFTFG"
     gene            complement(2654..2809)
                     /locus_tag="VF222_02150"
     CDS             complement(2654..2809)
                     /locus_tag="VF222_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141259.1"
                     /translation="MRHEKNVILFSMTTLIVGMQNSVLVLESSEIPITFHARSPQNPY
                     NITTYTV"
     gene            complement(2806..3219)
                     /locus_tag="VF222_02155"
     CDS             complement(2806..3219)
                     /locus_tag="VF222_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7141260.1"
                     /translation="MIYITISMSVETITAKLWQEKVIDSVLPLIVNFSAVRCGFCQAL
                     EPLYNRLSEQYKDNLVFLKLMVDDKNNQELIDRLSIEGTPTLKFYCKNREIGEHIGYA
                     IEPIIKQKIEIFLSEMEFCIKNSTPLPKNIQDNTR"
     gene            3480..3902
                     /locus_tag="VF222_02160"
     CDS             3480..3902
                     /locus_tag="VF222_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7141261.1"
                     /translation="MPEMSKDEIRNFLLQGTFPGKLGTINKNGTPHVVPIWYTLDEEV
                     NIIFNTGKNSVKAKNIQRYNRVRLCVDDQVPLYSFVIIDGVAEIISKEPNELFKWAKI
                     IAARYMDSDKAEEYGRRNSSEEELLIKIKPTKLLVRKI"
     gene            4219..>4492
                     /locus_tag="VF222_02165"
     CDS             4219..>4492
                     /locus_tag="VF222_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7141262.1"
                     /translation="MIKSYKDPLKRIQLSLFVFVLGAILYCSIIMLAMVGISPIQLST
                     ATTTSNTTLDEVTFLNNKGMSLNSLGKFNESITYFDKVLSMDPKNII"
BASE COUNT         1582 a          638 c          747 g         1525 t
ORIGIN      
        1 agttatggcc tgtgattcta agttatggaa caagttttat aattttaggt tttttttgga
       61 ttgcccatga ttatcagttc cactacgtta aacgagcaaa tcgaaccttt ctctggataa
      121 ctatctttta tctaatgttt atagcatttg taccattttc aacatctctg attggagagt
      181 atggagatca gcagatctcg gtaattatct atgcagtcaa tataagcata attggatttt
      241 gggaatatat acgatggcgg tatgctacaa aagatcatca tttagtagat agcgatcttg
      301 atcccacctt tataacaata atgtcaagaa gatttctttt aggacctact atttatttga
      361 tcgcagttgc tatatctttt ataagtactc aggtaagcct tgtactgttt atagctactc
      421 ctctatattt tctagtatcg gctaaaaagg acaaatcctg attttggttt accaaaaata
      481 agtaaatgaa acgaaatgga ttcatgggct tttttgacag gatataataa gaatgagtaa
      541 attcataatg gtctagagtg ttaatagcta ttgaattgaa tcatatatcc atatggttgt
      601 tgttattcct caagatgtac cttcaaccat ttagtttctt tcaaactgtc atctatacag
      661 tattatgacc ccaattggtt agcgggcttg gaggtgtgtg aactgatttt gacacaaatt
      721 tgtagttggt aatactttaa ctaaggattc tattacaaag catgagatat ttgatggtgt
      781 agaattaagt tctattgaat atattttcat ctaaatttaa aatctatttg aaataaatat
      841 tagtgtatag gttaggtaac ataattaata aagtttttga taatttaaaa ttaactaatt
      901 gtaacacaat ttgtaattag atttaaatag aaattgatta ctataaacct atagtccgta
      961 ttttgtaaat tatgttaatc attttttgga tcaaatttta acgtctggtg tagtgctgta
     1021 aaagctactg acattacttg ttaaatgatg gattagggtc atttgcgttt cggcgtttat
     1081 ggagtatttt attgcttttt attaaactaa gaacaaaagc taagaacaaa agccaaagct
     1141 aaccattgtg attttcatat cgaatagaac ataaaaatgc tttaatggaa atgagaatcc
     1201 ttatttactt ttgccacgat gtacatgcgt gtgatgaaag actgaaagaa tggagcggaa
     1261 cttaaacaat gaatcgagat aatattagtg cattagtggt tgcaataatg gtactatatg
     1321 ttatctttag ttttacaata tatttcaaca accctggtct taataaggta tggccaatac
     1381 aacaaaaaga caaagacatg ctcgtacttg gtcttcattc acctgaattt acgtttgaaa
     1441 agaattatac caatgtgaag actgctgtcc aaagatttgg gataactaat cctgcagtag
     1501 cagatacaac gatagaaaat cataccgtaa ctatgaaaat caataagact cagttccagc
     1561 aagttaatga gtcacaattc aaaaaggcac ctgaatttgc tcaaataagt ggctacatca
     1621 atacacctaa taataatccg ctaacacttt catctcttaa aggaaaagta gttttagtat
     1681 acatttggac atacacatgc attaactcga ttcgtccaat gccgtacatt gacgattgga
     1741 atcaaaaata ttctaataag ggtttagtca tagttggtct tcattcacct gaatttacct
     1801 ttgaaaagaa ttataccaat gtgaaggctg ctgtccaaag atttgggatt agttatcctg
     1861 taatactgga tagtgaccat ggaacttggg atgcttatgg aaaccagtat tggcctaggt
     1921 tttacttgat tgatactcaa ggaaatataa gatacgatca cataggtgaa ggtagttatg
     1981 atcaaataga aaaagcaatt cagttgctgc tagcagaacg tgctgctcta atgggtgcaa
     2041 aagagataag cttcaataca aaacctacta cagtaattaa accaggaagt ttgtattatg
     2101 tagatttaag acagagcaca acaccggaaa tatacatagg gtatagtaca gccagaacac
     2161 cccttggaaa tcctgaaggc ttcaaaccag atcagacagt ttcatactca atcccatcta
     2221 atacaaattt caaacttcat atagcttatc ttcaaggcaa ttggaaaaac aaccctgata
     2281 atatgcaatt acaaagtgat actgggcgca ttgttttgac atattatgca aaatctgtaa
     2341 atataatagc aggaggaaag ggtggaggag ctgtttttaa tgatgaagga atagaaggaa
     2401 caacagctgc agcatcaaca tcgaacaaat cactaggcga ggatttatca tctgatggta
     2461 gtttcagaat agacggacaa aggctgtata atttagctat acataacaac tacgctgttc
     2521 ataatattgt aattgatgtt aaaggtaaag gatttcaatt ctatacattt actttcggat
     2581 aggtggaggg acgatacgac agtatcaaga ccttattaat aggaatcagc cctttttcgc
     2641 tgccacctaa tatttataca gtgtaagtag tgatattata ggggttctga gggcttcgag
     2701 catgaaatgt aattgggatc tctgaagatt caagcacaag aactgaattt tgcattccaa
     2761 ctataagcgt cgtcattgaa aaaagaatta cattcttttc gtgtctcatc tggtattatc
     2821 ttgaatattt ttaggtaatg gagtactatt cttaatgcag aattccattt ctgataaaaa
     2881 tatttctatc ttttgtttta ttattggttc tattgcatat ccaatatgtt cgcctatctc
     2941 tctattttta caataaaact ttaatgtagg tgtaccttca atcgataaac gatctattaa
     3001 ctcctgatta tttttatcat ctaccataag ctttagaaaa actaaattat ccttgtattg
     3061 ttcagacaat ctattatata atggttctaa agcttgacaa aaaccacaac gaactgcact
     3121 aaaattaact attagaggaa ggacagaatc tatcactttt tcttgccaga gtttggctgt
     3181 tatggtttct accgacatag aaatagtaat atatatcata cttttttatt tttctataat
     3241 aggtataata ttagtactct atttatgtga atttataatt gaaatagtat aacatgcaaa
     3301 ataagatcat ctaaagacaa aaattccagg gtagtagtta attctcttac attttaaagg
     3361 ggagattatt cattccatag aataattcgt tactttataa ttaatacaat tttgtagtta
     3421 aagattttag tgatagattt gatctaactt ataattattt tatccaacta ataatactaa
     3481 tgccagaaat gtctaaagat gaaattagaa attttctttt gcaagggaca tttccaggga
     3541 aacttggaac aattaacaag aatggaacac cacatgtagt tccaatatgg tatacacttg
     3601 atgaagaagt taatatcatt tttaatacag gtaaaaattc agtaaaagca aaaaatattc
     3661 aacgttacaa tcgagtccgt ctatgtgtag atgatcaagt tcctctttac tcatttgtta
     3721 ttattgatgg tgttgcagaa ataattagta aagaaccaaa cgaactattt aaatgggcta
     3781 aaataatagc ggcaagatat atggatagtg ataaagccga agagtatggc aggagaaata
     3841 gttcagaaga agaattattg ataaaaataa agccaaccaa gttattggtc agaaagatat
     3901 agcaggattg taattaaatt aatttttttt ataaataaat tattgattca taaactaatg
     3961 aatcctgatt aaaaaaaact ttaattgaaa gacaaatggg ttctgtaagc ttctagcatg
     4021 atctgtaatt gggatcggta acaaaatatt taaaatttta tgttacttgt aactttaagt
     4081 ttttattatc atagatttgg aaaatagttg ttatattttt tattgaatat aggttaattg
     4141 atatagttaa gaatcaacca catatggttt tatatttgct aaaactatta atattatacc
     4201 tttctcaaca atgctgttat gataaaaagt tataaagatc ctttaaaaag aatacaatta
     4261 tcattatttg tttttgtatt gggagcaata ctttattgtt ctataattat gctagctatg
     4321 gtaggaatat ctccaataca attatcaaca gctactacta cttcaaatac aacattagat
     4381 gaagttacct ttcttaataa caaaggtatg tcacttaata gtttaggtaa atttaacgag
     4441 tctataactt actttgacaa ggtgttatct atggatccaa aaaatattat tg
//
LOCUS       DASVEV010000041        26135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_10526, whole genome shotgun sequence.
ACCESSION   DASVEV010000041 DASVEV010000000
VERSION     DASVEV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 26135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26135
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10526"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF222_02170"
     CDS             <1..468
                     /locus_tag="VF222_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141263.1"
                     /translation="RKKYEEMLKNNLCEKVVITRMETEKLRMRKSTDKKTDVAFMFER
                     NPNLRNDDVIFMDNKKMILLTLESEAVAIITFKSYYEDKENIFPLSVRIGHSLGNLHR
                     PIKVTKNQVIFPIQAETELEMLKKMFSSFSKFIDIRSDNMIFEPDEGSNIHEH"
     gene            458..1246
                     /locus_tag="VF222_02175"
     CDS             458..1246
                     /locus_tag="VF222_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX7141264.1"
                     /translation="MSTRFGSKIGVIDNSSNMNYSPIAADDTAIPVDPLVDEVSMLQL
                     GDSFFPTGMYTTSSGLETLFYDGRIKTKQEIIEFITVCLKYQIGPADCAALANAYDFA
                     KEENITKIIEIDHILFSMKLIKEIREASTRSGRQLLNCLLSFVPDNNLISSYYNSIKN
                     KTANGIYPVALALASNSLNIKKERACLILLYGFTVSITGAALRLGMLQHIEGQQVIHE
                     LKPIITTVINNNINRHFSHLWQFSPGIDLVQMKHERMDSKMFIT"
     gene            1268..1927
                     /gene="ureG"
                     /locus_tag="VF222_02180"
     CDS             1268..1927
                     /gene="ureG"
                     /locus_tag="VF222_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX7141265.1"
                     /translation="MTFKRIARVGIGGPVGSGKTMLIEQFVPLLSQIGYRSAIISNDV
                     VSKEDADRMRRNLASERGLMPESLVIGLATGGCPHTAVREDPSMNLSVVEEIESAHNQ
                     ELDLIIIESGGDNITTTFSPALADYFIYIIDVAGGDKYPRKRGLGIENCDLLVINKID
                     LAPHVGADLKIMERDAKLIRKDKPFVLVNCKTGEGIKEVVNNIVKDVLFDIPPKIQKT
                     E"
     gene            1933..2862
                     /locus_tag="VF222_02185"
     CDS             1933..2862
                     /locus_tag="VF222_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX7141266.1"
                     /translation="MKDLQYFIPDDIPQEVLSYESELSQLSVGKAGKLGALFLLLEKN
                     DKVNKTVIKEQFSKVPLFTQRALYLEESLPSMAYIYIISPSGGVLQGDRYRIDIKLAK
                     NAIAHITTQGATRLYRMEKNFATQILNISLDSNCYFEYVPDQIIPYRDSRFYQSVNLN
                     LHDDATLIYSETIVPGRMAMGESFDYDVCYLKTIAKSHSGSLKLVDTALLEPKKNKLK
                     RFGILEEFNMLSNMYLLVPSKYVSILHEKINEDIIKYNKVVARCSILPNDSGIVVRIL
                     GNMIDNIKEVIHHILKICRKEILGAGFSGIRKN"
     gene            complement(2930..3079)
                     /locus_tag="VF222_02190"
     CDS             complement(2930..3079)
                     /locus_tag="VF222_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141267.1"
                     /translation="MNALDKSRKKQTSWCLDCDKVFDTKLDAEIHKEILNHRLKITEF
                     WTISR"
     gene            3352..3843
                     /locus_tag="VF222_02195"
     CDS             3352..3843
                     /locus_tag="VF222_02195"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7141268.1"
                     /translation="MQSPFKNLKKTSNESKAETTLMMLPSDANPKGNVFGGVILKNVD
                     LIAGIVAKRHANQSNVVTASIDRMTFIKPIFIGNSLILEARLNYVKRSSMEIEVNIFA
                     EDLDYNIRVHAGRAYVTTVSIDKYGKSMEVPQLLLETEDDKRRFKEGEARMISRLKEA
                     GKL"
     gene            4017..4571
                     /locus_tag="VF222_02200"
     CDS             4017..4571
                     /locus_tag="VF222_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7141269.1"
                     /translation="MNKFLSLGIIVLVGSVLLITGIHVFGLAYAQENLSFTATFSGKE
                     VVPPVKTGGTGIANFEVSENSLSYQINILNTGKVTSVQIHRGTAGTNGDVLATVFKPK
                     GNGGDLIDDMPKILDISSITQRSSSFSFSGNFDESALTGPMKGNTISDLVLAMQSGET
                     YVVVHTEDYPKGELRGQILKMKVN"
     gene            5106..5348
                     /locus_tag="VF222_02205"
     CDS             5106..5348
                     /locus_tag="VF222_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7141270.1"
                     /translation="MEEIIPRYFELSIIPVWLKGKTRDEIAQAFGISQGTVSNIIAKI
                     RNEWDNYDADFMRELAKELREQDIIPDNCAYNLIFQ"
     gene            5571..6137
                     /locus_tag="VF222_02210"
     CDS             5571..6137
                     /locus_tag="VF222_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141271.1"
                     /translation="MKTPKWICEECEQTFTRRWNANRHCNNKHGTLDSVILFREYLAN
                     KGNNRYLRLSDMYSQNTNQIPYHQNLSFHEKTNSRLETKFKIPLHRIEDYQDREILLN
                     YKLDELAPQYQQLENLLSHVAQPKRASILGPLLNMALNSENPVAFINNQLDEFRRAKT
                     RDRMLDEISAYLGLNKNVTKEYLRISLK"
     gene            complement(6650..7831)
                     /locus_tag="VF222_02215"
     CDS             complement(6650..7831)
                     /locus_tag="VF222_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7141272.1"
                     /translation="MKISSIIFSKVIFTLLLARIQYTINWFNISSIFYFIALDFNLDI
                     NTLGLITTSFLIGVGLFQVPAGILVAKYGPKRLSIIGILLSSSMALVSGFSQDALQIT
                     ISRFVIGVGMAFFFGASVTLISNQLGKGNEGLGVGLLNSGHAIGALLGIFGWVALTEI
                     TGWRLSIIIGGIMGLAIVLIMRIGLLKEDNKKDFQIKSNELKNVLLNKSLIILGITLL
                     GYQTGASLTLTFIVLFLIEEIHINPIIAGLIGSLSLIIGIIISPISGKIYDKFKNAKK
                     LLFISAMLSALSIMLISTNSIYIIIIALITCGIFLSIGFVVIYARARESKKIIPEYHT
                     LSVGYVNGISLFGSFWIPLIFSFTVSQFNYTTAWLCAGITIIILSLPILRLDSEKRRD
                     S"
     gene            8138..8485
                     /locus_tag="VF222_02220"
     CDS             8138..8485
                     /locus_tag="VF222_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141273.1"
                     /translation="MDNHSNVNKKDNFDKNDPFGPGMLFWQNSVSNFISIYNQFIQDT
                     QRMNQVYRESAEITKEMGSLYKELALRIEKMNNLYRESTEITERMSKSWLDNILKSLL
                     PFKEKEQNEREKE"
     gene            complement(8569..9168)
                     /locus_tag="VF222_02225"
     CDS             complement(8569..9168)
                     /locus_tag="VF222_02225"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEX7141274.1"
                     /translation="MKIMVIYFFSLIMITTIFSLIGLTDLNIKAFAIADSNSFLTYNN
                     SKFNFSIQYPSTWDISENKFHPALENISSTIEIKSPFEGSQDLLQEKFLISLNKLQEN
                     ITFNDYVDNALNEFKNGYRDFKLISNNSTMIDNHNGKKLAYSYIAGVDPVSIKLIMNH
                     NIIPDGNKVYVLSFATPPDKYYEYLSIIQKMINSFKILK"
     gene            9913..11082
                     /locus_tag="VF222_02230"
     CDS             9913..11082
                     /locus_tag="VF222_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7141275.1"
                     /translation="MSLQIFTIKKIPIKLHFTLIIVFFLISWTLATGFMPHYFPNLTT
                     LQYWTMGIIGAIILFVSILLHELSHSLLSIRYGIHVNQILLFIFGGISDIKEETKEFK
                     KEFKIAVVGPLTSYALSGLFWMFFILVSYLNDTQQNIGNYLSSIEGILLYSSMINLII
                     GSFNLIPAFPLDGGRMLRAGLTKWKNDFDLATSIASKIGIAISFVIIGLGFVVIVRGS
                     FLGGFWLIIIGWFLNNGAQTYLAQNELSTRLKGLKLKEIMNRNFVAVKPDLKLSELIK
                     DYFNVYWKSAFPVINDDNQLVGMITTDAVSKKGKIENADKKVADMMIPKSELIVMSPD
                     KEVNDALKQLFRKRMSRIFIVDKQSQLIGLVSKSDILNLAQERKEFLQTSKKWNY"
     gene            complement(11361..11753)
                     /locus_tag="VF222_02235"
     CDS             complement(11361..11753)
                     /locus_tag="VF222_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141276.1"
                     /translation="MTNIKKILLVDNEVDITYALTNALENEEFNVESFNDPIIALNSY
                     KSNFYDLIILDIKMPNMDGFELYNKIREKDPKVKICFLTASELFYEEFRKARFLLVEK
                     IGEEYFIQKPIKTDDLVQKINALITQNE"
     gene            complement(11763..13658)
                     /locus_tag="VF222_02240"
     CDS             complement(11763..13658)
                     /locus_tag="VF222_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141277.1"
                     /translation="MEQTIKTEILKKLEIVAKLKEKNFQDVFEQNSEKLNLVSSRYQL
                     KVELDNYNNNKNNKSESQKIMNKIIKAIKPEVTKFEDIIILDLSGKVIASTNNTYLNT
                     IHNNDTYFIEGKKHNNVTILYKDKNQKLKSYLTGPLILNDKLLGVVTIFYDLGDLLSM
                     FKVFEEQVATGEINLVKKDGNGDALVINPLRFAPEASLNLTVSKDKIETPIIQSLLKN
                     EKTFPQAKDYRGMDVLAVTRYLEKLGWGLVVKMDKAEAFVPLENQKYVTILIGVLVGI
                     AIITTSTFLGNSISKPIQKLRDVMNDVAQGRFDSKIDVKGTQEIEDLAKQLEVMRFNI
                     SNTNTHLNEIVNERTKKIENALSNIKEKEEALEESNKNLLLANEKLSLQSKIQRDFIN
                     ITAHELRTPIVPIITLSELLYSIIKKENKTQKNPSKENEKKQEFLEVILRNCYRLYRL
                     TEDVLDVTKIESQTLKMNKEIFQLNEIIKNVVKDYREIINKKRYGSDQISIVFEPSKD
                     IIFVNADKGRIIQVLSNILDNALKFTKEGKIIITIKKVKENQEVMVSIKDSGTGLDPE
                     IIPQLFKKFATKSEQGTGLGLFISKNIIEVHDGIMWGENNSESNGATFYFTLPIWQSN
                     KTFEDGE"
     gene            14750..14875
                     /locus_tag="VF222_02245"
     CDS             14750..14875
                     /locus_tag="VF222_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141278.1"
                     /translation="MELTMAATERNSLELDNLLDKEDPFDNLVNEISKKIKKKNK"
     gene            complement(15119..15694)
                     /locus_tag="VF222_02250"
     CDS             complement(15119..15694)
                     /locus_tag="VF222_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141279.1"
                     /translation="MKLRYIASETICLIIIIIAITLVASNFTMIFSQSTTDSLTFDDP
                     LLGVKFQYTDEWLKEGSSLYGAKTECPSLPCMRFPVITVSMYPIVAEDFSLDNYTSEQ
                     RVSHEFAEGYKPLALNNTKIGEKNATQYIYTTKSPFLMEEASNDIINYEIYTTEGINL
                     YKISFASILDEQFDKNLNSFKKIKDTFEIAR"
     gene            15928..18048
                     /locus_tag="VF222_02255"
     CDS             15928..18048
                     /locus_tag="VF222_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7141280.1"
                     /translation="MSPRLLFFSILIVIAIGDVIILSAEPENKIIYANWVLLINSSAA
                     AIISFYIVYKEKKFRSDKTNFLLTVGLLLWFAANIVWSYYEVVLDVVSPVPSLADIFL
                     LSAYGFLIYRLVIVYKNLKGKVNKKTFFILGTITIVFLAYMFSLTLDLSNLSTSRGKI
                     FFIITFLYPLLNSVLAFLAILILIGIRHEKIHYITWLCELLALLSLVVGDSWFAIIVL
                     TELVEQLWISSLLLSAHYIIIAGGLIWYIKYLVPNKIPSILNTKKIFFNRQDKIKIAI
                     FFPLLILTISISFVIIYGLVEYDEDHLTKNIIPTQIAGTGIKTEIIGAILPKSGSFAS
                     TGIPTITSLKIAEQDVNEFFQKTNSSLRVKLQIEDSKTNPTETLNAIKRLDKANAKII
                     VGPMTSTAVNAVMNYSNENDIVLISQSSTSPALSIVGDNLFRFVPDDKNQGEFIAQKM
                     WDSGIKIVVPMWRDDIYGNELYKSMKENFEKLGGIVSEGVRYKPHIGNFAASLHRINF
                     IMWNQDLKQLSQLVEHQTKKVGNDSVGVYLISYDEVTPIMIQANEHTILDQVRWFGAD
                     SIAQNNHLLRNKDSALFAIKTNFVNPLFTISLQNEKIEHIREEVEKGKHEPTLLTYPI
                     ISYDAFWVAALSLDKSHTMLEKFNIDTLKENIVKLTGPYVGISGNITLNNAGDRTSGP
                     YELWRIADDDHQNHFKWTTRVPIL"
     gene            complement(18944..20353)
                     /locus_tag="VF222_02260"
     CDS             complement(18944..20353)
                     /locus_tag="VF222_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX7141281.1"
                     /translation="MEKHILSILIVTLFGMTIFMISHLFELKISPFILFSSIVMVSIY
                     VILSFEIIHRTSIAIFGALILVIASLLLGIILPEETLHFVIEVIDFNTIGLLLGMMII
                     VAILGETGIFNWVGIKAIRLSKGNLWKLMIILCVFTAITSMFVDNVTIILLMVPVTLS
                     IFKALKISPIPFILGQTLSSNIGGAATLIGDPPNIIIGSAANIDFSSFFINMAPSILV
                     TFLLGLVLIKIIFRKELKTIVNISGKFKTVREESLIKDKSLLKSSIIVLSGVIFLFVI
                     QGAIGIEVSIIALGGAATLLIITRVHVEKVLQEVDWATLIFFAGLFVVVGILEESGLI
                     ALLAKIVLGITGGHPWATFHVVIWLSAIASAFVDNIPFTATMVPIIETLNLSSSIDST
                     FGNFSISPLWWALALGADLGGNGTLIGSSAGVVAVGISLKYGQKISFIQWFKIGFPFM
                     ILTVALGSVILTLMTFLSL"
     gene            complement(20666..20941)
                     /locus_tag="VF222_02265"
     CDS             complement(20666..20941)
                     /locus_tag="VF222_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141282.1"
                     /translation="MNESEVEVNLLCKNCQHQISIHKPNCSALFHNSTCDCNKPEFYN
                     VVISGKYIGWTEIHCNSCGKLVAFIDSPIENIDDFKIRCKNCIIHHH"
     gene            21315..21596
                     /locus_tag="VF222_02270"
     CDS             21315..21596
                     /locus_tag="VF222_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141283.1"
                     /translation="MTNGTTFLVFYRFRPLDSEEKNIAKQEWEDIKSSLPDNIELIGE
                     YNHAWGTEYNGFLMFQSETSDAFFEWWAGFKDKIRWYVEKTHTIVARRK"
     gene            21663..22193
                     /locus_tag="VF222_02275"
     CDS             21663..22193
                     /locus_tag="VF222_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7141284.1"
                     /translation="MIILICGLPGTGKTFLSNKLSSYVNATVLTTDKIRKELLGKPTY
                     AAWERALVYDVLFLLAKYLHSSGIRCILDGTFNMEKSRSEVKELLNLTNDQFYIVECI
                     CPEDIIITRLLLRQDDYSDANVSIYLKMKKIYEPVRQKHITINTEKPIEKNIEKILKY
                     IYRSTADNVIKSFNEE"
     gene            complement(22318..22479)
                     /locus_tag="VF222_02280"
     CDS             complement(22318..22479)
                     /locus_tag="VF222_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141285.1"
                     /translation="MASISLSGKVKNNAFLLKKVFSYGFSGFSIMFIGYILINILNGY
                     PPRLIGISP"
     gene            complement(22670..23545)
                     /locus_tag="VF222_02285"
     CDS             complement(22670..23545)
                     /locus_tag="VF222_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7141286.1"
                     /translation="MHSNVNGKTHQYDHAKKLKLVLIITISFLVVEVITGIFTNSLAL
                     IANAIHMFTDVAGIGLSLFAINFSSKRSATAHKTYGFYRIEILAALINSTIILIFSFY
                     ISYEAYWRLFDQKEIEGLYVFVIATIGLIVNLIGIKILHKHAKENINIEGAYLEVFKD
                     MYGSCAVIVAALIIISTNLYIVDPIISIGLSLFILPRTWSLMKRVVHILMEGVPSNIS
                     YEQVKKSILEVKGVTGIFDLHLWTITSGFDAMTAHVVISDISKSQVILKEINSILEKK
                     YNITHNTIQIETYHE"
     gene            24340..24546
                     /locus_tag="VF222_02290"
     CDS             24340..24546
                     /locus_tag="VF222_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141287.1"
                     /translation="MSEEEIKKITTQAYVSNNKEFQKQAIDTAATYGKIAITHLLDLN
                     NRLSDDDINKYILSKIQEIKTKNP"
     gene            24764..25351
                     /locus_tag="VF222_02295"
     CDS             24764..25351
                     /locus_tag="VF222_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141288.1"
                     /translation="MRLIIIAIIFIIAFVIGVGLGISISLSMNPQTSSIGNIGIFFSG
                     LGVLFSGLNFWSIISKEIKLEFGKITIDNNKSYFIRVQQKDGKVKGKAINAESRLTLE
                     NTKYEYAPSVWANGRKKVVNIGEHEDLFLFQLQNNTTTIILPSAYEEKGFEPNTYNYD
                     DVSEINLKIEIFFDNGSEPKTYIKSIKEIVNSVSL"
     gene            complement(25565..25984)
                     /locus_tag="VF222_02300"
     CDS             complement(25565..25984)
                     /locus_tag="VF222_02300"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7141289.1"
                     /translation="MKPIDIKPPFDEETAKAKVKAAQDVWNTRNPELVAKAYTIDSSW
                     RNRIEFFKGREAIIAFLKRKWAKELDYRLMKELWCYNKNRISVRFEYEWRDADNPSQW
                     MRTHGNEHWEFEDSGLMRIRDMSANDYPIKESERRYR"
BASE COUNT         9608 a         3649 c         3768 g         9110 t
ORIGIN      
        1 aggaaaaaat atgaagagat gttaaaaaat aatctttgcg aaaaagttgt aataactaga
       61 atggaaacag aaaaattaag aatgagaaaa agtacagaca agaaaacgga tgttgctttt
      121 atgtttgaac gtaacccaaa tttaagaaat gatgatgtaa tttttatgga taacaaaaaa
      181 atgatattat tgacgcttga atcagaggct gtagctataa tcaccttcaa aagttactac
      241 gaagataaag aaaatatttt tccactctct gttaggattg gacatagtct cggtaattta
      301 catagaccta taaaggtaac aaaaaaccag gtaatttttc caatacaagc tgaaacagaa
      361 ctggagatgc taaaaaagat gttttcatca tttagtaaat tcattgatat tcgaagtgat
      421 aacatgatct ttgaaccaga tgagggatca aatattcatg agcactagat ttggtagtaa
      481 aataggcgtt attgataata gtagtaatat gaattactct cctattgctg ctgatgatac
      541 tgctattcct gtagatcccc ttgtagatga agttagtatg cttcagcttg gagattcatt
      601 ctttcctact ggaatgtata ccacatctag tggtctagaa acattgtttt atgatggaag
      661 aatcaaaact aagcaagaaa tcatcgaatt tataacagtt tgtttaaaat accaaatagg
      721 acctgcggat tgtgccgctc ttgcaaatgc gtatgacttt gctaaagaag aaaatattac
      781 taaaataata gaaatagatc atattttatt ttcaatgaaa ttaataaaag agattcggga
      841 ggcttcaaca aggtcaggaa gacagctttt gaattgctta ttgagctttg ttccagataa
      901 taatttaatt tcttcatatt ataactccat aaagaacaag actgcaaatg gtatatatcc
      961 agttgcacta gcactggcat caaatagttt aaatataaaa aaagaacgtg catgtctaat
     1021 tttattatat ggatttacag ttagtatcac aggtgctgca cttagacttg gaatgttaca
     1081 gcatatagaa ggacaacagg ttattcatga gctaaaaccg attattacaa ctgttataaa
     1141 caacaatata aatagacatt tctcacattt atggcagttt agtccaggta tcgatcttgt
     1201 tcaaatgaaa catgaaagaa tggattcaaa aatgtttatt acataaatta ttattcatta
     1261 tcttgctatg acatttaaaa gaattgctag agtcggtatt ggtggtccag taggttctgg
     1321 taagacaatg ttgatagaac aatttgttcc attattaagt caaataggat atagatctgc
     1381 tattatttcc aatgatgtag tttcaaaaga agacgctgat agaatgagaa ggaatttagc
     1441 atcagaaaga gggcttatgc ctgaaagttt ggtaattgga ttagctacag gtggatgtcc
     1501 ccatactgca gtaagagaag atccctccat gaatctctcc gttgttgaag aaattgaatc
     1561 agctcataat caagagttag atttaataat tatcgaaagt ggtggagaca atattacaac
     1621 cacattcagt cctgctcttg ctgattattt tatttatatt attgatgttg caggtggaga
     1681 taaatacccc cgaaaacgtg gtcttggaat agaaaactgt gatctgttgg taattaataa
     1741 aattgatcta gcaccacatg ttggggcaga cctcaagata atggaacgag atgccaaatt
     1801 aattagaaaa gataaacctt ttgttttggt aaactgtaaa actggggagg gaattaaaga
     1861 agtagtaaat aatatcgtaa aagatgtttt atttgatata ccaccaaaaa tacagaaaac
     1921 tgaataatta ccttgaaaga cctgcaatat tttattccag acgatattcc tcaagaagta
     1981 ttatcttacg aatcagagct tagtcaacta agtgtaggaa aagcaggaaa attgggggct
     2041 ctatttttac tactagaaaa aaatgataaa gtaaataaaa ccgttatcaa ggaacaattc
     2101 tcaaaagtac ctttatttac tcaaagagct ctgtatcttg aagaaagtct tccttctatg
     2161 gcctatatat acatcatatc tccatctggg ggagtgctac aaggggatag gtatagaatt
     2221 gatataaagt tagctaaaaa tgcaatagct catataacta ctcaaggagc tactcggctc
     2281 tacaggatgg agaaaaattt tgcaacacaa atcctgaata tatcattaga cagtaattgc
     2341 tattttgaat atgttcctga tcaaattata ccttatagag attcacgatt ctatcaatct
     2401 gtgaatctta atttacatga tgatgcaacc ttaatatatt ctgaaactat agttcctggt
     2461 cgaatggcta tgggtgaatc ttttgactat gatgtatgtt atttaaagac tatagccaaa
     2521 agccattctg ggtcattaaa gcttgttgac acagcattac tagaaccaaa aaaaaataaa
     2581 ttaaaaagat ttgggattct ggaagaattt aatatgttat caaatatgta cttattagtt
     2641 ccatccaaat atgtaagtat tctacatgaa aaaatcaatg aagacataat aaaatacaat
     2701 aaagtagtag caagatgttc aatattgccc aatgattctg gaatagtagt aagaatacta
     2761 ggtaacatga ttgataatat aaaagaagtc atccatcata tcctcaaaat ctgtagaaaa
     2821 gaaattcttg gagctggatt cagcggtatt aggaagaatt agattttata tttttttatt
     2881 ttagaaaata tatttttaaa atacacaagt gtatatgtta attggttatt tatcttgata
     2941 ttgtccagaa ttctgtaatt ttgagtctat gatttaaaat ttctttatga atctcagcat
     3001 ctagtttggt atcaaaaact ttatcacaat caagacacca tgatgtttgt ttttttctac
     3061 ttttatctag tgcattcaaa tgataaaaca ctgatctaat gttaaataaa cgattcgata
     3121 tttaaaacct gaaatgaaat aaattgtata attttaatac atcgaagata aattttaatt
     3181 aattcctatt atattatctt taaaatttac taaaatacaa atttcacagt tataactttt
     3241 acaatatctt gttagtacta agctactatt atttatatta gaacaaaaaa tttggtatga
     3301 ttagttagtc tttatatatt cataattatg agtacattga ggtatctata tttgcaaagc
     3361 ccatttaaga acctaaaaaa aacctctaat gaatcaaaag cagaaacaac actcatgatg
     3421 ttaccatcag acgccaatcc gaaaggaaat gtatttggcg gtgttatatt aaaaaatgta
     3481 gatcttatag ctggtatagt cgctaaaaga catgctaatc aatcaaatgt tgttactgca
     3541 agtattgata ggatgacttt tattaaacca atatttattg gcaactcttt gatacttgaa
     3601 gcaaggttaa attatgttaa acgttcttca atggagattg aagtaaatat ttttgcagaa
     3661 gaccttgatt ataatataag agttcatgca ggaagagcat atgttactac cgtttcaata
     3721 gataaatatg ggaaatctat ggaggttcca cagcttttat tagaaacaga agatgacaaa
     3781 cgacgattta aagaaggtga agctagaatg atatctcgtc taaaagaagc aggaaagctt
     3841 tgagtttaat ttgatgagtt aattattatt ttatgattat tcagtgaaaa tgaaagttga
     3901 ggtcgtagtc gtcaataaat tattccgtaa tatatacatg aatcctatgc aatcatttca
     3961 taagtttttt ataatctgat agaaatctaa ttaaataata ggatatctat tagaatatga
     4021 ataaatttct ttcactggga attattgttt tagttggttc tgttctatta atcaccggaa
     4081 tacatgtgtt tggtttggcc tatgctcaag aaaatcttag ttttacagca acattctccg
     4141 gaaaagaagt agtacctcct gttaaaacag gcggaacagg gatagcaaat tttgaagtct
     4201 cggagaattc cttatcctat caaataaata tattaaatac aggaaaagtt acctctgttc
     4261 aaattcatag gggtacagcg ggaacaaatg gtgatgtcct agccaccgtg tttaagccta
     4321 aaggtaatgg tggagatcta atagatgata tgccaaaaat tttagatatt tcgtcaataa
     4381 cacaacgatc tagctccttc tcttttagtg gaaattttga tgagtcagct ttgacaggtc
     4441 caatgaaagg caatacaatt tctgatcttg tgttagcaat gcaaagtggt gaaacgtatg
     4501 tagttgttca tactgaagat tacccgaagg gtgaactccg agggcaaata ttaaaaatga
     4561 aggttaacta actggtattc agaagtctaa taaaggcttt agagaaaata tattctagaa
     4621 aaactagagc tggttagaaa tctacatact atttctttta atagcctctt gtgaaaaagt
     4681 gaaaaactat aactatactc tgtaacttta gtccaaatct attattctgt ttataatttt
     4741 gcaatataca tattaattct tcttattctg taatagctac aaattacatt ttttagatcc
     4801 tccaatgtag gattatagtt agacagtgta ctgtatttcc ttgtttccaa caacatgttc
     4861 tactgtatta aattaagaag aaccaattgg aaaattatta catatttgta tattatagaa
     4921 tatactgaaa ttataaatct attcaaataa atgaaattaa aataattatg ttttctttat
     4981 tatagattaa ttacgcataa aatatttgac gaacttattg acacattagc caaagacaaa
     5041 ataaatatga cggaatattt gacgaatatt ctatttaata atattggtaa aaaccaaata
     5101 attgtttgga agaaataatt ccaagatatt tcgaattaag tataatccct gtctggctta
     5161 aaggaaaaac tagggatgaa atagctcaag cattcggtat aagtcaagga actgtatcga
     5221 atataatagc aaaaataaga aatgagtggg ataattatga tgctgacttt atgagagaat
     5281 tagcaaaaga attacgagaa caggatatta tacctgataa ctgtgcttat aatttgattt
     5341 ttcaataaac ttgaaaacat aaagatacaa agcagagata gaaaaatttc ttgagattcc
     5401 aactttactt tcatcacaat ttgataaatg attcgtcaaa aattctggat tatctttttt
     5461 gcattgttaa aattttttat cattaataaa atttattcgt cataagtgaa aagagttact
     5521 ctaagtaact ctttatgaca gtttaagagt tactttacta taattaaaat atgaaaacgc
     5581 ctaagtggat ttgcgaagag tgtgaacaaa cctttacaag acgatggaat gcaaatcgac
     5641 attgcaataa taaacacggg acgctagact ctgtgatctt atttagagag tatttagcga
     5701 ataaaggaaa taatagatat ctacggctct cagacatgta ttcacaaaat actaaccaaa
     5761 tcccatatca ccaaaatttg tctttccatg agaagacaaa ttcaaggtta gaaacaaaat
     5821 ttaagatacc attgcaccga attgaagatt atcaagatag agagatttta ttaaattaca
     5881 aattagatga attagctcca cagtaccaac aattagaaaa tcttctttcg catgttgctc
     5941 agccaaaaag ggcgtctatt cttggacctc ttttaaatat ggcactaaac tcagaaaatc
     6001 ctgttgcttt tatcaataat caattagacg aatttcgacg cgccaagacc cgagacagaa
     6061 tgctggatga aatatctgca tatcttggtt taaataaaaa tgtcacgaag gaatatttga
     6121 ggataagttt aaaataagtc ctgaaacaag acagaaataa ctttagatgg tgatattatc
     6181 agtgaagttc ctgaaaaacc tcctgatgca aatgacattt attggaaatg gcattctgag
     6241 ataatatttt aacatatatg ttaaataata aaactaacat taattatatc gtataacaat
     6301 acttaagata atggataata gattttttat ttgctcttat tggtaatgtc taatctactt
     6361 ttgaaatatt tctttaaata tatttttagt attcatggga atgttgatcc tattttatga
     6421 taggttccaa taccgtgtat ttaatcgaaa aaatagtatt gaaccagtta aaagagctgg
     6481 taaattttat atttatttga cttttttata aaaaaaatca tttaatgtat aataattatc
     6541 aacttaaatt tttttatatt attattattt ttttgtcgtt atatgcaatc tagtaattag
     6601 aaattcgaaa attctaaata tttccttttt ttctttcaaa gtattctgac taactatctc
     6661 tccttttttc ggaatctaac cttaaaatag gaagggataa tattattata gtaattccag
     6721 cacataacca tgctgtcgta taattaaact ggctcacagt aaaggaaaat ataagtggga
     6781 tccaaaagga accaaataac gatatgccgt taacataacc tacggaaaga gtatgatatt
     6841 cgggaataat tttttttgat tctcttgctc tggcatatat taccacaaac ccaatcgata
     6901 agaatattcc acaagtaatt agggctatta tgataatata tatggagttg gttgaaatta
     6961 acataatact taaagcagat agcattgctg aaataaacaa aagtttcttt gcattcttaa
     7021 atttgtcata tattttacct gaaattggtg atataattat tcctattatt aggctcaaac
     7081 ttccaattag gccagcaata attgggttaa tatgtatctc ttcaataagg aataaaacaa
     7141 taaaagttaa tgtcaaactg gctccagttt gataacccaa caaagtaata ccaagtatta
     7201 ttagagattt atttaatagt acattcttta attcgttaga tttaatttgg aaatcttttt
     7261 tattatcttc ttttaataat cctattctca ttattaatac gattgctaag cccattattc
     7321 cgccaataat gattgaaagc cgccagcctg tgatttctgt aagtgcaacc caaccaaaaa
     7381 tccccaaaag tgctccaatt gcatgacctg aatttaataa tcctacacct aaaccctcgt
     7441 ttccttttcc taactggttt gatatgaggg taacacttgc tccgaaaaaa aatgccattc
     7501 ccacgccgat tacaaatctt gaaatagtta tttgtaacgc atcttgagaa aatcccgaaa
     7561 ccaatgccat acttgaagat aataatattc caattatgct gagtcgttta ggaccgtact
     7621 ttgcgactaa aattccagca ggaacttgga ataatcctac gccaatgaga aaactagtag
     7681 taattaatcc aagtgtattt atatccagat taaaatctaa tgcaataaag taaaaaattg
     7741 aagagatatt gaaccaatta atagtgtatt gaatcctagc taataataat gtaaaaatta
     7801 ctttagagaa aataatggaa gaaattttca ttaaatgatc aaaattccga caggaaaaat
     7861 ttgtttattc ctaagtaagt agtattaaaa tttattctgc ataaaaatag actgatggtc
     7921 tgcaatttgg aaatttctac tgaacacatg tgaagttgca ctgttattta atgatgctta
     7981 tttcaaatat aattctacat aaaacataca atatataata ttttttatta aaaaaaaatg
     8041 gctatatcaa gttatatcca taccatccaa atacaatttt aataataaat gaagatttta
     8101 tagaaagaat aaaaacacac atcgaaacta tatagctatg gataatcatt ctaacgttaa
     8161 caaaaaagat aattttgaca aaaacgaccc ttttggccct ggaatgcttt tctggcaaaa
     8221 ttctgtttca aatttcatct ctatatataa ccagtttatt caggacactc aaagaatgaa
     8281 tcaggtgtat cgtgaatcag cggagattac aaaggaaatg ggaagtctgt ataaagaatt
     8341 ggccttacgc atagaaaaga tgaataacct ttatcgtgaa tcaacagaga taactgaacg
     8401 aatgagtaaa tcatggttag acaatatctt gaaatcactg ttaccattta aagaaaaaga
     8461 acaaaatgaa agggaaaaag aataagaaaa ggaagacgac aagtatagtg aaaatttata
     8521 aaaaaattaa gaaaaaataa ataaacaata ttcttctgtt tttggatact actttaaaat
     8581 tttaaacgaa tttatcattt tctgaattat actcaaatat tcataatatt tatcaggagg
     8641 agtagcaaag gaaaggacat aaactttgtt tccatccgga attatattgt gattcattat
     8701 caattttata cttactgggt ctactcctgc tatgtatgag tacgctagct tcttcccatt
     8761 atgattatcg atcattgtag aattatttga aataagttta aaatctctat atccattttt
     8821 gaattcattt agagcattgt caacatagtc attgaatgtt atattttcct gtaatttgtt
     8881 taggctaata agaaattttt cttgcaataa atcttgagat ccttcaaaag gtgacttaat
     8941 ctctatagta ctggaaatat tctccaatgc gggatggaat ttattctcag atatatccca
     9001 agttgatgga tattgtattg aaaaattaaa ttttgaattg ttatatgtta ggaatgaatt
     9061 agagtcagca attgcaaagg ccttgatatt taaatctgtc aagcctatta aggaaaaaat
     9121 tgttgttatc ataattaaac tgaaaaaata aataaccatt atcttcatta cttgaaataa
     9181 atcatttcct ttatttataa taacttatca tttaacataa atgaaacaat atttattgtt
     9241 tttataatag attgtcttct caaatcaaat agaaggagga aaatatgttc ttttaagaac
     9301 aatgatagca atatactggc atagttgtac taacaaaaat attctgatta ttattgtcac
     9361 gataggaatg ttaattcgat tcaaacaata tcaagtgtaa tttatttata attgatgaga
     9421 gatatatgat tattacagaa ttctaaaata ttgcaaaaag tattactata agaaaaaaat
     9481 tttctattaa taattataaa gaaattcttt aactatacaa atttaatagt tgctttttga
     9541 gaaagtttta ttctaaatcc ttatattgca tataatgtta aattaatcat gctacgaaaa
     9601 tattcattat actcctttta tcttgctctc aaagtgaggc tctttcgttt aactaaaaga
     9661 aaacaaaatc agccgtatac cttatcatca tagaataact tcgagttcta taacctatta
     9721 ttttaatgat ttacaaatgt aatatttata acaaatttta attagccaca tctttaataa
     9781 tcttatttca agctatatga taagttaaaa aaattataat aaagtgagaa atcgaagagt
     9841 tttatgatgt taatttatca acccaaataa taattataat ctttaaatga tattctcgct
     9901 acgtatctct atatgtcttt gcaaattttt acaataaaaa aaatacctat taaattacat
     9961 tttacactca ttatcgtttt tttccttatt tcttggactc tggcaacagg attcatgcca
    10021 cactattttc ccaatctaac tactttacaa tattggacca tggggataat tggtgcaata
    10081 atattatttg tttcaatatt actccatgaa ttatctcatt cacttttatc aattcgatat
    10141 ggtattcatg tcaatcaaat acttcttttt atttttggag ggatctctga tataaaagag
    10201 gaaacaaaag aatttaaaaa ggaatttaaa atagctgtag ttggtccatt aaccagttat
    10261 gctttatctg gattattttg gatgtttttt attctagttt cttaccttaa tgatacccaa
    10321 caaaatattg gcaattatct cagttctata gagggaattt tattgtatag tagcatgatt
    10381 aatctgataa ttgggagttt caatttgatc ccagcatttc cattagatgg tggacgaatg
    10441 ttaagagcag gattaacaaa atggaaaaat gattttgatc tagctactag tatagcttcg
    10501 aaaataggca tagcaatatc atttgtaata ataggattgg gatttgttgt tatagtaaga
    10561 ggatcatttt taggtggatt ctggttaata ataattggtt ggtttcttaa caacggtgct
    10621 caaacatatt tagcccagaa tgaattatca acaaggctta agggtctaaa gttaaaggaa
    10681 ataatgaata gaaatttcgt agcggtcaaa ccagatttaa aattgtctga attaattaaa
    10741 gattatttta atgtttattg gaaaagtgca tttcctgtga taaatgatga taatcaactt
    10801 gtggggatga ttacaactga tgctgtatct aaaaaaggta aaatagaaaa tgcagataaa
    10861 aaagtcgctg atatgatgat accaaaatct gaattaatag taatgagtcc agataaggaa
    10921 gttaatgatg ctttaaaaca attatttaga aaacgaatga gtaggatatt tatagtagat
    10981 aaacagtcac aattaattgg attagttagc aaatctgata tactcaattt agctcaagaa
    11041 agaaaagaat tcttacaaac ttcaaaaaaa tggaattatt aatagagtat aagcaactaa
    11101 tgaactcact aacctaatgt actcatttat gctttgttgc atgattcatt atgtaacgat
    11161 aataataaaa tcaaattatg agaaaaaacc tgtagataaa aagataatta caaatactgt
    11221 aaaaaattaa taaattaata aaaaaactag taaaattgtt ttataaatga aatagtatag
    11281 tgaataaaat taaattgcac tagtttagac acgattatat gagttttatt aaccataaaa
    11341 ttttatctca aaattgtaga tcactcattc tgcgtaatta atgcatttat tttttgaacc
    11401 agatcatctg ttttaattgg tttctgaata aagtattctt ccccaatttt ctcaactaat
    11461 agaaatcgtg ctttcctgaa ttcctcataa aacaattcac tagcggttaa aaaacagatt
    11521 ttcactttgg gatctttctc ccttatctta ttgtataatt caaatccatc catgtttggc
    11581 atttttatat ctagaattat taaatcataa aaattgctct tgtaagagtt taatgctatg
    11641 atggggtcat taaaggattc tacgttgaat tcttcatttt ccagggcatt agttagtgca
    11701 taagtgatgt caacttcatt atctactaaa agtatttttt ttatattagt tattttatac
    11761 tctcactctc catcttcaaa tgttttgtta gattgccaga taggcaaagt aaaataaaaa
    11821 gtagctccat tactctcaga attgttctct ccccacatta taccatcatg aacttctatt
    11881 atattcttgg atataaagag accaagtcca gtaccttgtt cagatttggt tgcaaatttt
    11941 ttaaataatt gtggtattat ttcaggatct aaacctgttc cactatcttt tatactaacc
    12001 ataacttcct gattttcttt tacctttttt atagtaataa tgatctttcc ttctttagta
    12061 aatttaagag cattatcaag aatattagaa agaacctgaa taatccttcc tttatctgca
    12121 ttaacaaata taatgtcttt gcttggctca aacactatgc taatttgatc acttccgtat
    12181 ctttttttgt ttataatctc tctatagtct ttcacaacgt tttttattat ctcatttaac
    12241 tgaaagattt ctttattcat tttcagggtt tgactttcta tctttgttac atctagtaca
    12301 tcttctgtaa gtcgatataa cctataacaa tttctaagaa ttacttcaag gaattcttgt
    12361 ttcttctcat tttcttttga tggattcttt tgtgttttat tttccttctt gattatgcta
    12421 taaagtaatt cactaagggt aataatagga acaattggag ttcgtagttc atgagctgtt
    12481 atatttataa aatccctctg gattttactt tgaagactta atttttcatt agctaaaaga
    12541 aggtttttgt tagattcttc taaagcttct tctttttcct ttatatttga tagggcattt
    12601 tcaatttttt ttgttctttc attgacaatc tcattcaaat gggtgtttgt atttgatata
    12661 ttaaatctca ttacctctag ttgtttagct aaatcttcaa tctcctgggt gcctttcaca
    12721 tcaatcttag aatcaaatct tccctgagca acatcgttca ttacatctct taatttttga
    12781 attggtttag aaatagaatt accaagaaat gttgatgtcg ttattatagc gattccgact
    12841 aaaactccaa tcaaaatggt tacatatttt tgattttcaa gaggtacaaa agcttcagct
    12901 ttatccattt ttacaactaa accccacccc aatttttcta aatatcttgt tacagccaag
    12961 acatccattc cacggtaatc tttagcttgt ggaaaagttt tctcgttctt taataacgat
    13021 tgaattattg gggtttctat tttatcttta cttactgtta aattcaaaga tgcttcagga
    13081 gcaaatctta aaggattgat aactagagca tcaccattac catccttctt taccaaatta
    13141 atttcacctg tagctacttg ttcttcaaat actttaaaca tagataataa atcacccaag
    13201 tcataaaata tagttactac acccaataat ttatcattaa gtataagtgg accagttaaa
    13261 tatgatttaa gtttctggtt tttatcctta tataaaattg ttacgttatt atgttttttc
    13321 ccttcaataa aatatgtatc attgttatga attgtattta aataggtatt attggtggaa
    13381 gcaattactt tacccgataa gtctagtata attatatctt caaacttcgt aacttcaggt
    13441 tttatggcct taattatctt attcataatt ttctgtgatt cgcttttatt atttttatta
    13501 ttgttataat tatctaattc caccttaagt tgatatctgc tagaaactag gtttaatttt
    13561 tcactattct gttcaaaaac atcttgaaaa tttttttctt ttaatttagc tacaatttca
    13621 agtttcttca agatctccgt ttttattgtt tgttccattt ctacataagt aaaaattcca
    13681 aataccatta atggtattaa agatattact aaaaaaaaat tatcagttta gatcgtatat
    13741 tcatggtatt tacctcagct atttttgtat aataaatttg atataaaaat atattgtttt
    13801 taataaagga taaataataa ttttagaata tatgacacgc tggaaccctt aatagcagag
    13861 gttcattgtt tcttttaata aaaatattga atgacataaa ataacaaacc aaatattata
    13921 agacttcccc atattagtaa aatatatcaa tttccattgg ttatctattt gtaaacaaat
    13981 gtaactgaaa ccaaaatggt ttgtggctgg taatgatatt tatttactgg ttacaatgat
    14041 taacgctgct gctttaacta aaaaatgata agattgaaat tgaaaataaa aaagtcgctg
    14101 atatgatgat accaaaatct gattaacagt aaagagtgga gataggaagt taattatttt
    14161 ttaaaacaat ttttaaacgt tgattgagtt gaatattggt agtaaatatt ttttcattta
    14221 cattattaat attgttaact tttaaaaaac ggtatttttt acaataaaac attaataaaa
    14281 cataatccat acctatacaa aattaatatc tggtaatgta attatatacg aaacttataa
    14341 gaagtgaaaa attttctaaa attgataaag tcaagaatgt gagtgttaat aacattgtca
    14401 ccgataatgc tcgtgtatgg tagtaattta gcgtatttga aatagaatta cttcggtgtg
    14461 gaatattagg attgtaagta ttaacgtgat tccctaaata taggggagac taatattatg
    14521 atataactta tttcgaattc attcaaaggt tagatttacc tattacctta attatttcta
    14581 caatagaaaa attcatgata aaaattttta accatgctta ataaaagtaa ttctataaat
    14641 tttatataac aagtagtgat gaaccgtaaa gtaccttgtt tatacttttt tgtaccaaca
    14701 aataaataat tacatttatt tatttaattg aatgcgaaga aaaaaactat tggaattaac
    14761 tatggctgct actgagagga attctctaga actagataac ctactagata aagaagatcc
    14821 atttgataac ctagtcaacg aaatatcaaa aaaaataaaa aagaaaaata aataattatt
    14881 ttttcatttt tctttttttc aagcataccc tagagtatta aatttgtact ccagatgatc
    14941 gttagaggtt gagccttaat gttagaaaaa aaatttggct attattatag ttcttaattt
    15001 taatttgtca tggtgttgat aaataaaact cttataatag aaaaaacttt tagcgactgt
    15061 aaccaaacct tctgattatt attgttgttg ataatttcct caaaatatat ttttcattct
    15121 atctagctat ttcaaaagta tccttaatct tcttaaatga gttaagattc ttgtcaaatt
    15181 gttcatcaag aattgatgca aaagatattt tatagaggtt aataccttct gtagtatata
    15241 tttcataatt tataatatca tttgaagcct cttccatcag aaaaggtgat ttagtagtat
    15301 aaatatactg agttgcattt ttctctccaa tctttgtatt attcagtgca agaggtttgt
    15361 atccttcagc gaactcatgg gacacccttt gttcactagt ataattatca agcgaaaaat
    15421 cttcagcaac gattggatac atactaactg ttattacagg aaatcgcata catggtaacg
    15481 aaggacattc ggttttagct ccataaagag aagaaccttc ctttaaccat tcatcggtat
    15541 attgaaactt tactcctagt aatggatcat caaaagttaa tgaatcagta gtagactgag
    15601 aaaaaatcat agtgaagtta gatgctacta aagtaatagc aataatgatt attattagac
    15661 atatagtctc acttgcgata tagcgaagtt tcattatatt catatgatca taattttatc
    15721 aatataaatt tttaaaattt caattaaagt ttaaagttct ctttatgaga atgactttgt
    15781 tcagtttttg agtgtgttag accattcctg gagtaatgca aatttcctca gtattttgcc
    15841 tttactaata ctaataactt cgattacttt attattaata aaagaagaat agtttataat
    15901 tcctttttta tttaagcaag agaaggattg tctccacgcc tacttttttt ttcaattttg
    15961 atagttatag ctataggaga tgttattatc ctttcagctg aaccagaaaa taaaataatt
    16021 tatgcaaatt gggttctatt aataaactca tcagcagcgg ccataatatc attttatatc
    16081 gtatataagg aaaagaaatt ccgttcagat aaaaccaact tcttactcac agtaggattg
    16141 ctattatggt ttgctgctaa tattgtatgg tcttattatg aagtagtttt agatgtagtt
    16201 tcgcctgttc catctctagc tgatatcttt ttactttcag cgtatggctt tttgatatat
    16261 agattagtaa tagtgtataa aaatttaaaa ggaaaagtaa acaaaaaaac tttttttatc
    16321 ttaggaacta ttactattgt atttttagca tatatgttca gtctaacttt ggatctatct
    16381 aatctttcaa cgtcaagagg gaaaatcttt ttcataatta cgttccttta tccgctctta
    16441 aattcggtat tggctttttt ggctatatta attttaatag gaataagaca cgaaaaaatt
    16501 cattatatta cttggttatg tgaattattg gcattattat cactagttgt gggtgatagt
    16561 tggtttgcaa ttatagtatt aacggaatta gtcgagcagt tatggatatc ttctctattg
    16621 ttatcagctc attatataat aatcgctgga ggtctaatat ggtatattaa ataccttgtt
    16681 cctaacaaaa tcccttctat attaaatacg aaaaagattt tttttaatag acaggataaa
    16741 ataaaaattg ctattttttt tcctttgctt atccttacta tttctatctc atttgttatt
    16801 atatatggtc ttgtagaata cgatgaagac catttaacta aaaatataat acctacacaa
    16861 atagcaggta ctggaattaa aactgaaatt attggtgcca ttttgccaaa atcaggatct
    16921 tttgcttcga caggtattcc aaccataact tcattaaaga ttgctgaaca agatgttaat
    16981 gaattctttc agaagactaa ttcttccctg agagtcaaat tacaaattga agattctaaa
    17041 acaaatccaa cagaaacttt gaatgcaata aaaagactag ataaggctaa tgcaaaaata
    17101 atagtaggtc ctatgactag tacagcagta aatgctgtta tgaactactc caatgaaaat
    17161 gacattgtac tgatcagcca gtctagtaca tcgcctgctc tatcgattgt aggagataat
    17221 ttattccgat tcgtccccga tgataaaaat cagggtgaat tcatcgccca aaaaatgtgg
    17281 gatagcggta ttaaaattgt agttcctatg tggagagatg acatctatgg aaatgaactc
    17341 tataaatcaa tgaaagaaaa ttttgaaaag ttaggaggca ttgtttcaga aggagtaaga
    17401 tataaaccac atataggaaa ctttgctgca agtttacata gaattaattt cattatgtgg
    17461 aaccaagacc ttaaacaatt gagccaatta gttgagcatc aaacgaagaa agtcggaaat
    17521 gattctgtgg gtgtctacct gatttcgtat gatgaagtaa ctccaataat gatacaggca
    17581 aatgaacaca caattttaga tcaagttaga tggtttggag ctgatagtat tgcacaaaat
    17641 aaccatcttt tacgcaataa agactctgca ttgtttgcaa taaaaacaaa ttttgttaat
    17701 ccactattta ctataagctt acagaatgaa aaaatagagc atataagaga agaggttgaa
    17761 aaaggtaaac atgaacccac attattaact tatccaataa tatcttatga tgctttttgg
    17821 gtagcggctt taagtttaga taaaagtcat acaatgcttg aaaagttcaa tatcgatact
    17881 ttaaaggaga acatagtaaa attaacaggg ccttatgtag gtatttctgg taatataaca
    17941 ctaaacaacg ctggtgatag aacaagcgga ccttatgaac tgtggagaat tgctgatgat
    18001 gatcaccaaa atcattttaa atggacaaca agagtaccta ttttataaag aactgtattc
    18061 tgaattgaat cttctaaatg tcatgaatac atataataaa tcttaattgt gaaattttgt
    18121 tttagctcat tattctatta atatgcaaag actaaaattc ttgtaaattc ccaatttaat
    18181 tactattatt atgcttcatt agttaataaa aattcacgtg ttgattatag agatattcct
    18241 cttgatgtat taataatctc agtcatactt ttttctatag ccaaaagtac aaaaattaag
    18301 ggtcatggga aaataccaga atgaaatgca aaaggtaatg ctgcaatcat tagtccaacc
    18361 ataactttaa ccaatgtaaa tagattttct aagttgagag caatggaggt agtaagtcta
    18421 aagatatcaa atcaagtatt ttatatgtcc aaaaaaaaat aataaagcaa caattgttgt
    18481 atatattata aaaataatac aaatagtaga aagtaattgt ctaaatttca agacactctt
    18541 acccactaaa tatttcatcc ctgacaattt tgtttctgga taatataatg ccataaccaa
    18601 acaaatatga agaaaactaa aaaatgctaa aatcgataca atgcatgtgg tacctctcat
    18661 aatgatatta aatagcattt acaattatag atcatctttg gtatcctttt taagctgagt
    18721 atattttcaa agcaaagatt tcataaaata tccaaaccct cttttttcgt caaaattggt
    18781 ttacgaacca ttgacatcat tcaatcaatt cacagatgaa tattcttttt ttgaaatctg
    18841 tataaccgtt tcgtattatc gacaagatta acttatcaac aaaactttga atgacaatag
    18901 acttcataca gtcttatctt tacaaataaa tagtgaataa taatcacaaa gataggaacg
    18961 tcataagtgt gagtataaca gacccaaggg caacagttaa aatcataaaa ggaaatccta
    19021 ttttgaacca ttgaataaag cttatttttt gtccatattt taaagagata cctacagcca
    19081 ctacgcctgc acttgaacca atcaatgtcc cattaccacc taagtcagct cccaatgcta
    19141 aagcccacca gagtggactt atactaaaat taccaaatgt agaatcaata ctagaactta
    19201 aatttaatgt ctcaatgatg ggaaccattg tggcagtaaa tggtatattg tcgacaaatg
    19261 cactagcaat agcagacaac caaatgacaa catgaaacgt agcccaaggg tggcctcctg
    19321 ttattccaag cacaatttta gcaagtaatg caataagtcc tgattcctct aaaatcccta
    19381 ccactacaaa aaggccagca aaaaagatta aagttgccca gtcaacttct tgcaagactt
    19441 tttctacatg tactctagta ataatcaaaa gagtagctgc tcctcctaac gcaataatag
    19501 atacttctat cccaatcgct ccttgaatta caaacaagaa aattacccca gataatacga
    19561 taatgctact tttaagaagt gatttatctt ttattaatga ttcttcacgc actgttttga
    19621 attttccaga tatattcact atcgttttta attcttttct aaaaattatt tttattaata
    19681 ctaatcccaa tagaaaagtt accagtattg atggtgccat attgatgaag aaagaactaa
    19741 agtcaatatt tgctgctgaa ccaataatta tgtttggtgg atctccgatt aaagtagcag
    19801 ctcctcctat attagatgag agagtttgac ctaaaatgaa aggaattggt gatattttga
    19861 gagctttgaa tatggataaa gtaactggaa ccataagcaa aattatagtt acattatcca
    19921 caaacattga ggtaattgca gtaaagacac atagaattat cattaatttc cataaattac
    19981 ccttagatag tcgtatagct ttaataccga cccaattaaa aataccagtt tctcctaaaa
    20041 tagcaactat tatcatcatt cctaaaagca atcctattgt attgaaatca ataacttcta
    20101 tcacaaaatg cagggtttct tccggaagaa taattcctag tagtaaactt gcaattacta
    20161 gaattaaagc tccaaaaatt gcaattgatg ttctatggat aatttcaaat gatagaataa
    20221 cataaattga aaccataact atagacgaaa ataaaataaa aggagaaatc ttcaattcaa
    20281 ataaatgaga aatcataaaa attgtcatac caaacaaagt tacaattaaa attgaaagaa
    20341 tatgtttttc cattgatatc acgtcgcaaa aattgccaga agagaataat acgtaaagtt
    20401 gtctatattt ctgatcaata gcatttttgt aaaagattca cctatgaatg tttaggatgt
    20461 tgtattcaat gaatagtcgg tattctataa gttgatatgt attattagat taatattaat
    20521 attttaattg ttatgtatat tttacgaaga atctattatt caatatttat ttatatttaa
    20581 ctttcatcca cgatactcta atgaaaggat tctattacta taaaaattct taaaatttat
    20641 taaataagat ggttattttc tgttctcaat gatgatgtat aatacagttt ttacacctta
    20701 ttttaaaatc atcaatattt tcaattgggc tatcaataaa tgccactaat tttccacagc
    20761 tgttacagtg aatttcagtc catcctatat actttccaga aattactaca ttataaaatt
    20821 caggcttgtt acaatcacaa gtactattat gaaataatgc cgaacagtta ggtttatgta
    20881 ttgatatttg atgttgacaa tttttacaca ataaattaac ctcgacttcc gactcattca
    20941 tactttaaaa caatccatct aaggaaataa taattaataa aagtacgtat tttcatatta
    21001 taatatagtg aatatagtat tcaattaaac tattatttat taaataaata tttctcttga
    21061 agaaggatca aaatctaggt caaatttggt ttacatccca atggtcccat tgtgatttac
    21121 ttctataatc tcatttactt aataaaatga tacattttat catttttaca aataaaaaat
    21181 gaaagaggca gaaagataaa actattaata ggaccaaaaa ctatttttgg tactcttaat
    21241 aaattaaaaa aattctttat aatgattaat gctggactat atctatttat ttatctacta
    21301 tttgatagaa gaatatgact aatggaacca catttttagt cttttataga tttaggcctt
    21361 tagattctga agaaaagaac atagctaaac aagaatggga agacattaaa agtagtttac
    21421 cagacaatat cgaacttatt ggagaataca atcatgcatg gggaacagaa tataacggat
    21481 ttctaatgtt tcaatctgaa accagtgatg cattttttga atggtgggca ggattcaaag
    21541 ataaaataag gtggtatgta gaaaaaactc atacaatagt agcaaggaga aagtaaagta
    21601 ataactgtat tgtattttct ttaattcaca cttgacgaat tatttcgata ttatttgaag
    21661 atttgataat tctaatttgt ggacttccag gaactggaaa aacattttta tctaataaac
    21721 tatcctcata tgtaaatgct actgtattaa ctacagataa aatccggaaa gaattacttg
    21781 gaaaaccaac ttatgctgca tgggaaagag ctctagtcta tgatgtatta tttttattag
    21841 caaaatattt gcattcatct ggaattaggt gtatcttaga tggaacattt aatatggaaa
    21901 aatctcgaag tgaagtaaag gaattactaa atttaacaaa tgatcaattt tatatagttg
    21961 aatgtatttg tccagaggat ataattatca ctagattatt attgagacag gatgattatt
    22021 ctgatgctaa tgtctccata tatttaaaga tgaagaagat ttatgaacct gttagacaaa
    22081 aacatataac aataaatact gaaaaaccaa tcgagaagaa tatcgagaaa attcttaagt
    22141 atatttatcg atctacagca gataatgtaa ttaaaagttt caatgaagaa taataacgat
    22201 tttgactaac ttattaaatg gtacagagga agaggttagt ttaaatataa atgctataaa
    22261 aacttgatat tctgagtaat tctaatttca atttacaaaa tgtaagagaa tattttttta
    22321 aggtgatatt cctattagtc taggtgggta gccgtttaat atattaatca aaatatatcc
    22381 aataaacatg attgaaaaac ctgaaaaacc ataagaaaaa acttttttta acaaaaaagc
    22441 attatttttc acttttccag ataaagaaat tgacgccacg ataaaataaa tcacaattgt
    22501 atatttctat ttttagaaac attaattggt aatatatttt taattattat taagtagaaa
    22561 tgaactataa attaattcta tatttttctt ctgaatcctt ttatgtactt attaagtaat
    22621 ttgtataatt ttttggctgt gaatctcagt agaatttaaa ttttaataac tattcatgat
    22681 atgtttcgat ttgaattgtg ttatgcgtaa tattatattt tttttccaat atagagttaa
    22741 tttcttttaa aataacttgt gattttgaaa tatcagaaat aactacgtga gcagtcatag
    22801 catcaaatcc tgatgtaata gtccataaat gtagatcaaa tattcctgtt actcctttaa
    22861 cttctaaaat tgatttctta acttgttcat acgaaatatt agaaggaacc ccttccatca
    22921 agatatgtac aactcttttc atgagtgacc aagttctagg taatataaat agtgataaac
    22981 caatactaat tattggatca acaatatata agtttgtaga tatgattatg agggctgcaa
    23041 caatcacagc acacgatcca tacatatctt taaaaacttc taggtatgct ccttcaatat
    23101 tgatattttc ttttgcatgc ttgtgaagta ttttaattcc tataagatta acaatcaaac
    23161 cgatagtagc aataacaaaa acatataatc cttcgatttc tttttggtca aataaacgcc
    23221 aataagcttc ataactgata taaaacgaaa aaattaatat tattgtgcta tttattaatg
    23281 cagctagaat ctctattcga taaaatccat aagttttatg agctgtcgca gatcttttag
    23341 atgagaaatt tattgcaaat aatgataagc caatacccgc aacatctgtg aacatatgaa
    23401 ttgcatttgc aatcaatgca aggctattag taaaaatacc agtaattact tctacaacga
    23461 gaaatgaaat agtaatgata agtactaatt ttaatttctt agcatgatcg tactgatgtg
    23521 ttttaccatt aacatttgaa tgcaaaagtt ttggtgtaaa ttaagaagta tagcatataa
    23581 gttttttcta tcgtatgggt catgaaagac gaataaagat tagtattata gtcataaata
    23641 taggatgatt cagtagagca aaactagata ttatcaagaa taactttatc ttatgaaagt
    23701 tttgtcatat tcctctgttt taatgtgagt ttctttcata ttaaaattag aaaagtataa
    23761 aatattgtga taggaaatat ttgtttaaat ttaatattta gattgaattt ataattaata
    23821 ctgaaagcaa taacccacta ctattagtga gggatatcca ataatagaag actatgaaaa
    23881 tcaatcctga tttatctttg gactgtattc accttcatga aacataattt taattctttg
    23941 atacaaatta acgactttat tagtattatt atattggttc tattaactta gcaactaatt
    24001 tttgatgtgt aaacaatcgt ctcatgtttt caacatattt aatatctttt ctaggaatac
    24061 tttgccttta ggcaaaattt gatagtaatt aacagcagcc ggtctattct cctagctcaa
    24121 accaaattcg ctttttacta attctgctat tcctaaagca ttaagatgaa aaggaataac
    24181 cttagttaat atccttgaca aaaacttctc ttgaacaact taaaacaatg accaaaatac
    24241 atttaaaaaa aggaactaga aatgtttgaa gcaattagac tttcaagaat ggtatataaa
    24301 tcatccatac tgtaagctga tagacgagat aaaggtatta tgtcagagga agaaataaag
    24361 aaaattacaa ctcaagcata tgtaagtaat aataaagaat tccaaaaaca agctattgat
    24421 acagcagcaa cctatggaaa gatagctata actcatctat tagatttgaa taaccgatta
    24481 agtgacgatg atataaataa gtatattttg agtaagatac aggaaatcaa aacaaaaaat
    24541 ccctaaatgt cttttttaaa aatgggtgtt atagtggcct tgtaacaccc actatgttta
    24601 catcttcaat aaacagcgat ggtagatgca taaatatata cacaataaac gattaaagga
    24661 ttttggtata tgggtgacgg aaaagttaat agctaaccga atttattaaa gcggtagcta
    24721 ttgaatataa agagctttac tatactatct cattcacatt taaatgagat taataattat
    24781 cgcaataata ttcattatag catttgtaat aggtgtagga ttaggaattt ctatttcctt
    24841 atccatgaat cctcaaactt catctatagg aaatattgga atattttttt ctggactagg
    24901 agttttgttt agtggtttga acttttggtc aataatatca aaagaaataa aattagaatt
    24961 tggtaaaata acaatcgata acaataaaag ttattttatt agagtgcaac aaaaagatgg
    25021 aaaagtaaaa ggaaaggcta ttaatgcaga atctcgtctt acattagaaa atactaaata
    25081 tgaatatgca ccttctgtct gggctaatgg aagaaaaaaa gttgtaaaca taggtgaaca
    25141 cgaagattta tttttattcc aattacaaaa taatacaaca acaattattt taccatctgc
    25201 atatgaggaa aaaggttttg agcccaatac ttataactat gatgatgttt ccgaaataaa
    25261 tttgaaaatt gagatttttt ttgataacgg aagtgaacct aaaacgtata taaaatcaat
    25321 aaaagaaata gtaaattctg ttagtctatg aaatgtgacg acagttaaca ctttgttaaa
    25381 gagagactct atgttaacag aaccataata atatatattt ataataagaa tcattgagta
    25441 acatatacta tataatttgt ttgttttgtt tgtttaaaga tcattattgt gttataatat
    25501 ttacttgctc ttcctttcat gcaaaataat actgaatatt atttcgtaac taactatatt
    25561 attttcacct ataccttctt tctgattctt taattggata atcatttgca ctcatatcac
    25621 gaattctcat taaaccacta tcttcaaatt cccaatgttc atttccatgg gttcgcatcc
    25681 attgacttgg attatcagca tctctccatt catattcaaa tcgtacagaa attctatttt
    25741 tgttataaca ccatagttct ttcatgagac gataatctaa ttccttagcc cattttcttt
    25801 ttagaaatgc tataattgct tctcttcctt taaagaattc aattctattt ctccaactgg
    25861 aatcaatagt atatgcttta gcgaccaatt ctggattacg tgtattccat acgtcttgtg
    25921 ctgctttgac ttttgccttc gctgtttctt catcgaatgg aggcttgatg tcaattggtt
    25981 tcatatttat ttctacaaga atcagattat tatttattac ttaattttta tagtctacta
    26041 tattactgca aaaacttttc attttataat tttataatat tttatcaata ttattaaaaa
    26101 tgggaacaac aagttatgat ggaatatata aaaat
//
LOCUS       DASVEV010000042         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_10563, whole genome shotgun sequence.
ACCESSION   DASVEV010000042 DASVEV010000000
VERSION     DASVEV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10563"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VF222_02305"
     CDS             complement(<1..2096)
                     /locus_tag="VF222_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7141290.1"
                     /translation="MTLSSLKDENLQKILSEIGYDSLYPIQEQAISNGLLTGENLLIT
                     SPTASGKTLIAIMAAFKKMEKKEKVIYMTPLRSLATEKYHDFLEFNSNFINNNKNLAT
                     VTKNIDFEKKNSLAKKKKGDKKKSFIVKLAMGNYDSAGNDLLNADIVILTNEKLDALI
                     RNDSELLSNVGLFVIDEVHLMGDKDRGPTLEMMITKIKRFYQEAQILALSATVSNSKD
                     IAEWLDCKLLENNWRPTELLEGVYDNGIVRMNNNTRIKIKTYYNFQSIPVSVAIDSLE
                     SGGQAIIFAETRKRAASLAAKSVPGVFRLLDKDQKEKAKKSALQLSKISDDTDITKNL
                     IQMVSQGVSFHHAGLSQPIREIIEDSFKQGVIKLLVATPTLAAGVNLPARRVVLSSVL
                     RYDYDIGTNIPISILDYKQFCGRAGRPQYDTFGEAIIIPESGMNSEELYDHYILGTPE
                     PINSKLFNEKALRFHILSTISTIPGMKKSELYDLFLNTLFAQNYRKSTIVFKVDIALE
                     SLETWNLIKSKNERYIATDFGKKTSILYIDPMTAIEFKNELDTLTEINTNESLKAITQ
                     PTQNEYNNLIIKFLHLISECSDFYPKLSLRKKDMDYFYNFVNKYMDEDLLFDLTEYNC
                     SRSFLALYEWINESSERNLSDRLGIEPGDMHRIAESADWLLYTLDEFAKLFNRNDILE
                     TLSDLRIRTKYGVKQE"
     gene            2321..2444
                     /locus_tag="VF222_02310"
     tRNA            join(2321..2342,2360..2375,2401..2444)
                     /locus_tag="VF222_02310"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2372..2374,aa:Ser,seq:cga)
     gene            2832..2978
                     /locus_tag="VF222_02315"
     CDS             2832..2978
                     /locus_tag="VF222_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141291.1"
                     /translation="MQGSSPTPGASDVGSTTKRNKNKQREFVFETKSNRYMILQKSQI
                     EENK"
     gene            3244..3468
                     /locus_tag="VF222_02320"
     CDS             3244..3468
                     /locus_tag="VF222_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141292.1"
                     /translation="MTKHDILNFLNKLRKPAVEDPMSKWIGSYNVRQIILTKINISIL
                     DIPERIQAQEYRTSAQCCPGILSLMFTYLK"
     gene            complement(3676..3903)
                     /locus_tag="VF222_02325"
     CDS             complement(3676..3903)
                     /locus_tag="VF222_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141293.1"
                     /translation="MKNSVIAIQSLVITTIVHALLVPTFFEMDNNAFGEGEYFVDDDK
                     DQMMYNQQVVYTKECKSPCPSSAEMCIAMCA"
     gene            4060..>4250
                     /locus_tag="VF222_02330"
     CDS             4060..>4250
                     /locus_tag="VF222_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141294.1"
                     /translation="MVNEKKEEETTPTANQFEKLATAGEKSALYMRTIDDFAHWVTVN
                     MMSNLDVEECTSILEDYIK"
BASE COUNT         1513 a          646 c          600 g         1491 t
ORIGIN      
        1 aattcttgtt taactccata ctttgttcta attcgaagat cggataaagt ttcaagaata
       61 tcatttctat taaacaattt agcaaactca tctaatgtat ataataacca atcagctgat
      121 tctgcaattc tatgcatatc accaggttca attcccagtc tatcacttag gtttctttca
      181 ctactctcat taatccattc atacaaagcc aagaaactcc ttgagcaatt gtattcagtt
      241 aggtcaaata ataaatcctc atccatatat ttattgacaa agttataaaa ataatccata
      301 tcctttttcc ttaacgaaag ctttggataa aagtctgaac actcagatat gagatgtaga
      361 aattttataa tgagattatt atactcattt tgtgtaggtt gagttattgc ctttaaggat
      421 tcatttgtat ttatttcagt taatgtatct aattcatttt taaattcaat agctgtcata
      481 ggatctatat ataaaataga agtttttttt ccaaaatcag tagcaatata cctttcattt
      541 tttgatttaa taagattcca cgtttcaaga gattctagtg caatatctac tttgaaaact
      601 atagtggatt ttctataatt ctgggcaaac aacgtattta aaaataaatc atataattct
      661 gattttttca taccaggaat agtagaaata gtacttagaa tatggaaacg taatgccttt
      721 tcattaaaaa gttttgaatt aataggttct ggagttccaa ggatataatg atcgtataat
      781 tcttcagaat tcatacctga ttctggtata attattgctt ctccaaaagt atcatattgt
      841 ggccttcctg ccctaccaca aaattgttta taatccaaaa tactaattgg aatatttgtt
      901 ccaatatcgt aatcatatcg taaaacactt gataacacta ctctacgtgc tggtagattt
      961 acgccagctg ctaaggttgg agttgctact agtaatttaa ttacaccttg tttaaatgag
     1021 tcttcaatta tttctcttat gggttgactt aatcccgcat gatgaaaact tacaccttgt
     1081 gacaccatct gaataagatt tttagtaata tcagtatcat ctgaaatttt tgacaattgt
     1141 aatgctgatt ttttagcttt ttctttctga tccttatcta ataatctaaa aacacctggg
     1201 acagactttg cagcaagtga tgctgcacgc ttacgggttt cagcaaaaat tatagcttga
     1261 cccccacttt ccaatgaatc tattgcaact gacacaggaa tagattgaaa attatagtaa
     1321 gtttttattt taattcgggt attattattc atacgaacta taccattatc atatacacct
     1381 tcaagtagtt cagttggtct ccaattattt tctaataatt tacaatctaa ccattcagct
     1441 atgtctttgg aattagagac tgttgcactt aaagcaagta tttgagcttc ttgatagaat
     1501 cttttaattt ttgttatcat catttccaga gttggaccac gatctttgtc acccattaga
     1561 tgaacttcat ctataacaaa taatcctaca ttggataata gttcactgtc atttctaatc
     1621 agtgcatcca atttttcatt agttaaaatt acaatatctg cattaagaag atcattacct
     1681 gcagaatcat aatttcccat tgcaagcttt acaataaaac ttttcttttt gtctcctttt
     1741 ttcttttttg ctaaagaatt tttcttttca aagtctatat ttttagtaac tgttgctaaa
     1801 tttttattgt tgttaataaa attagagtta aattctaaaa aatcatgata tttttcagtt
     1861 gccaaagatc taaggggtgt catatagatc actttctctt ttttctccat ttttttaaaa
     1921 gcagccataa ttgctatgag cgtctttccg cttgctgtag gagatgttat aagtaaattt
     1981 tcgcctgtta aaagtccatt agaaatagct tgttcttgaa ttggataaag tgagtcatat
     2041 ccaatctctg aaagaatttt ttgtaagttt tcgtctttta gtgatgatag cgtcatgttt
     2101 ccttatcaaa ccatataatt tttgattatt atttgttatt ttgaatgacc taatgtagtt
     2161 ataataagat taatttaagg tttggaattt tttttctatt tctcttcata tatttactat
     2221 attattgcaa atcgggtaaa aatgtaataa tatatgaacc aagataataa tgagttattt
     2281 ataaaatata tattaatatc aatgaaatat tttaatgaat gccggggtac ccaagctagg
     2341 taagtcaaat gaccttaaaa ggggtcagcc tcgagatcat gaaattgtaa ttgacatgct
     2401 agctgatgtc cttggactcg tgggttcgag tcccactccc ggcgtccaac tcgtggtcat
     2461 attaaagaat ttgctgaaat atctagtatg agaaatagat tatggtatta aagaaatcac
     2521 aagaaataga actaaatatt gtaaagttta tataaagaaa ttgttgcttt ataaaaccta
     2581 atttaaaaca ataaataaaa cctattgatg aaaaagatat ttcacgtttg attttgcttt
     2641 attatagacg actaacagag tcctctgttt taatcgaact attataggat tgaaattaga
     2701 gcctgttgat aagttctgca cctcaatata ggttttaatc aactagtttt accaattgta
     2761 gaatattgaa atattagtta tgactaaatt tttgtttaaa atttttaaaa tgtataaacc
     2821 cgcgaatgta gttgcagggt tcgagtccca ctcccggcgc ttcggatgtg ggttctacta
     2881 caaaaaggaa taagaataag caaagagaat ttgtctttga gacaaaaagt aatagatata
     2941 tgattctgca aaaatcacaa atcgaagaaa ataaataata ataacaatct tttttcttct
     3001 agcaacacag aagaagaatt agaaagaaaa atagattcta taacaactgg actatcaaga
     3061 ccgtatttca acaaaatatt aaaggaatta gtcaaaaaga atctagaaaa tgcaactatt
     3121 atttgtgatt atattattgc agaacagata gaaataaaca ttcaaaattc aactaaagaa
     3181 agtaaaataa aagttcttac atggctatct aaccattttc aagacgagaa atcgtttagg
     3241 aacatgacaa aacacgacat tctcaatttt ctcaataaac ttagaaaacc agcagtagaa
     3301 gatcctatga gtaaatggat tggatcatat aatgtaaggc agataatttt aaccaaaata
     3361 aatatttcta tattagatat accagagaga attcaagcac aagaatatag aacgagtgca
     3421 caatgttgtc caggaatact aagtttaatg tttacatatt taaaataaca tatccattta
     3481 tgatctataa ttaaaaaaca aataaattat tgaatttaaa cattcaaaat ttgttcccaa
     3541 aaactcaaac cagaaagatt ttcaaaattg tgtatgatca ataaaataat tcaaatttct
     3601 aaaagaccat tcagtttctt gactaacgct atttcagttc ctagaatgtc tttcccaata
     3661 taaaaaaata ggttgttaag cacacattgc aatacacatc tctgcactag atgggcaagg
     3721 agatttacat tctttagtat agactacttg ttgattatac atcatctgat ctttatcatc
     3781 atcaacaaag tattctcctt ctccaaaagc attattatcc atttcaaaga atgtagggac
     3841 tagaagtgca tgcactattg tagttatcac taaggattga attgcaataa cagagttttt
     3901 catatatttt ccctcaatat atatacagca tatttgaata tgagtatttt aaaacatgga
     3961 tagacaaata tttccagaaa tgttttcgaa aaaagttatt tttttcccta ccaacaaaaa
     4021 taaaaatata ctaaaatcat atggatttgt tagattaata tggtgaatga aaagaaagag
     4081 gaagaaacaa ctccaacagc caaccaattc gagaaattag ccacagcagg agaaaaatca
     4141 gcactctaca tgagaacaat agatgatttt gctcattggg ttactgttaa tatgatgtct
     4201 aatctagatg tggaagaatg cactagtatc ttggaagatt atataaaaag
//
LOCUS       DASVEV010000043         6776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_11116, whole genome shotgun sequence.
ACCESSION   DASVEV010000043 DASVEV010000000
VERSION     DASVEV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11116"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF222_02335"
     CDS             <1..316
                     /locus_tag="VF222_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141295.1"
                     /translation="NMFIDNSGNKENITLPKTVRTLPNTVRFQNQGNALFSDQIYQGT
                     TKKNINILDIEFWEDYEGYLKKSYRESSIRCRLLYAKQYHYVITEENARNILGLPHNK
                     KV"
     gene            480..1082
                     /locus_tag="VF222_02340"
     CDS             480..1082
                     /locus_tag="VF222_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7141296.1"
                     /translation="MFLNNYEKEKRVIELHLAGKTIREIAKEVHMSFTPISKIIKTYE
                     RKKELQAKREESNQSSQIKKPSLSSRAFKLFIDGKKPTEVVIELDIPPEKVEKLWSRF
                     LKSERMEECYDFFQMCQYDIPTILSINNFIKKNNIQGKDIANVLRKANGVINLNQTIL
                     NLKGEIERLKQMKNNYTLNQNTMKNLQPVPLGPLPRYYNW"
     gene            1497..1655
                     /locus_tag="VF222_02345"
     CDS             1497..1655
                     /locus_tag="VF222_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141297.1"
                     /translation="MSVLEHEWIIIFEAVNAHDIENLCIDVGIASFNTIKNVALKKYE
                     DIHHKVTK"
     gene            1762..2445
                     /locus_tag="VF222_02350"
     CDS             1762..2445
                     /locus_tag="VF222_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7141298.1"
                     /translation="MKGIIFDMDGVLIDAMPFHAEAMKIAIKEETNHEIDKKIVYQLE
                     GMPSHNLVKEIFKKNNINKELDQKLVEKISERKKQVFKEIENTRLIDGVHDLIQELNK
                     CNCLKAIVTGAAKKEIELTIDKMIGLKNFDVVISGDDVETGKPDSTPFVVALKKMNLK
                     SSECIIVENAPLGVEAANKAGIRYILTLNNTPLDISSDFNNLISKGTKIFQDTKSTID
                     FLRKWCCNN"
     gene            3286..3936
                     /locus_tag="VF222_02355"
     CDS             3286..3936
                     /locus_tag="VF222_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7141299.1"
                     /translation="MSTELLMSVFQTLQDLMRIPDTFQNDIITAVIALFVVIDPIGNI
                     PLFIALTKKLEKEEQKTVSKTAILTAGILLIIFGVAGSQILQLFGITIFSFMIAGGIL
                     LFITAIELLTYGEWRFASNVKGEAGVVPIAFPLLAGPGAITAVIILYQTSGFLITVLS
                     IIIVIAITYVILRMVNPIYKILGNRGSMIVSRVFAVIIAAIAVEYIVEGIKNLFVT"
     gene            complement(4171..4791)
                     /locus_tag="VF222_02360"
     CDS             complement(4171..4791)
                     /locus_tag="VF222_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141300.1"
                     /translation="MQINTLVIFLLICSIAPVFIGKQVFSQQEKLENWKLDSLLSENK
                     SEKGNLLVQLKSSLTSDPFNFPIEIVFLNSTLPKQTPQSVPNLESNNSGDTFSSAGLS
                     VPSTLERVVPIQSYDIFIYDADGNELWKKTSQSATQGRANLNVEFGDYNGKLTIVIDK
                     ILPSKSTNNILNTDLPSRIENADNMSNADQSSRFDSVNFTSSLTKA"
     gene            5139..5510
                     /locus_tag="VF222_02365"
     CDS             5139..5510
                     /locus_tag="VF222_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141301.1"
                     /translation="MSKAVDKEIKQLYLCIVVIAVSIVSMGSYLNVIAQEGSIIKLSD
                     ISDNFKALLLNNQSQISNQTAIAIASLQNEEKTLSVNALENLPRGSTLINTIVNAPMK
                     IYQLEQIGRIVENGLMINQTS"
     gene            5996..6421
                     /locus_tag="VF222_02370"
     CDS             5996..6421
                     /locus_tag="VF222_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7141302.1"
                     /translation="MSKKSVVSIQTNSNVTALDITKLMVKNKVGSVLVIDSTNFPIGI
                     ITERDIIKKVCVTNTLPNDMNLEKIISSPVITIMSYDSIETAAQKMTDYKIKRLPVLE
                     ENQQIVSILSLTDITKHLSKILIDDYKRFGFLKSIFESD"
BASE COUNT         2598 a          889 c          954 g         2335 t
ORIGIN      
        1 aaatatgttt atcgataatt cgggaaataa ggaaaatata accttaccaa aaacagtaag
       61 aaccttacca aatacagtaa gatttcagaa tcaaggaaac gctctatttt cggatcagat
      121 ctaccaaggc acaacaaaga aaaatataaa cattttagac atagagtttt gggaagatta
      181 cgaaggatat ctaaaaaaat cttataggga aagtagtata cgatgcaggc tgctttatgc
      241 aaaacaatat cattatgtca ttacagaaga aaatgcgcgt aatatccttg gattacctca
      301 caataaaaaa gtgtaaaata atttttatat gtagtatcta ttttcaaaat tttccacgac
      361 tgatgaaatc tagatgataa cagtagtaac caattgataa cataaaataa caaacgataa
      421 caatgatgat agtatttaat aactactttt ttaggagata ttatagaagt gtttcaacct
      481 tgtttttaaa taactacgaa aaagagaaaa gagtaataga attacattta gcaggtaaga
      541 caataagaga gattgcaaaa gaagttcata tgtcatttac ccctatatca aagataatca
      601 aaacatatga gagaaagaaa gaattacaag ctaaaagaga agaaagtaac caatcaagtc
      661 aaataaaaaa accatctttg agtagccgag catttaaatt atttattgat ggtaaaaaac
      721 ctactgaagt tgtaattgaa ttagatattc cacctgaaaa agttgagaaa ctatggtccc
      781 gatttttaaa atcagaaaga atggaagaat gttacgattt ttttcagatg tgtcaatatg
      841 atataccaac aatattatca ataaacaatt ttatcaaaaa aaataatatc cagggaaaag
      901 atattgcaaa tgttttgaga aaagccaatg gtgttattaa cttgaaccag acaattttaa
      961 atcttaaggg agaaatagaa agattgaaac aaatgaaaaa taattataca ttaaatcaaa
     1021 atactatgaa aaatcttcaa ccagttccat taggccctct accaagatat tataattggt
     1081 gattattagg aattttttat tttcttaact ttttatattt tcaatagaac ttatcatcaa
     1141 gcaatgacaa cgaccaaaca tttagtactc caataaacct aagcaattct cccgtattgt
     1201 ctaccgatcc tcaaatcgaa gcagagggaa ataatgtcta tgttacaggg gaagaaggac
     1261 caactaacca agaggaaata tttttcagca tattaagatt atgaatgtta taggtaatat
     1321 ccctaattct gtaaagtcat aataatggat caaagtctat ttggtgcctt tgctaaatat
     1381 attcctgaat cttgtcctct taacaatttt taaagtaaaa aaatatttct tgaaattaaa
     1441 gacaaactaa aaaagaataa gtcaaaatat agagtaaata aattgaatct ttttacatgt
     1501 cagtactgga acatgaatgg ataataattt ttgaagcagt caatgctcat gatatagaaa
     1561 acctatgcat tgatgtagga attgcctcct ttaataccat aaaaaatgta gcactaaaaa
     1621 aatatgaaga tatacaccac aaagttacta aatgaagatc taatatttct gataatacta
     1681 caatttaatt gattattcat tttatattcc aatgacatat tcactttaat ataccatctc
     1741 tgatcctcat tgtattaaaa tatgaaggga ataattttcg atatggatgg tgttttaata
     1801 gatgcaatgc cctttcacgc tgaagcaatg aaaattgcta taaaagagga aaccaaccat
     1861 gaaatagaca aaaaaatagt ttatcaatta gagggtatgc cctcccataa cttagtaaag
     1921 gagattttta aaaaaaataa tataaataaa gaactagatc aaaaattggt agaaaaaata
     1981 agtgaacgaa agaaacaggt atttaaagaa attgaaaata ctcgtcttat tgatggagta
     2041 cacgatctta ttcaagaact aaataaatgt aattgcttaa aagcaattgt tactggtgca
     2101 gcaaaaaaag aaatagaact aacaatcgat aagatgatcg gattaaaaaa ttttgatgta
     2161 gtaatttcgg gcgatgatgt tgagacagga aaaccagatt ctactccatt tgttgtagca
     2221 ttaaaaaaaa tgaatctaaa gtcatcagaa tgtataatag ttgaaaatgc ccctttagga
     2281 gttgaagcgg cgaacaaagc aggtataaga tacattttaa cacttaacaa cactccactt
     2341 gatatttcct ctgactttaa caatctcata tcaaaaggaa caaaaatatt tcaagatact
     2401 aaatctacaa tcgatttctt aaggaaatgg tgttgtaata actagcagtt tattagataa
     2461 aatcttgaga aattatatat cttttccttt tactggttaa tttataataa cacatataaa
     2521 gaaaaaaaga tattgaaaaa aatttggcac ataaaggtaa acatgttatt gtaaaaaact
     2581 acataggatc tggttaaacg attttattca gagattctaa taaaatagta cattatgaaa
     2641 ttcattaaac tgattaatta gactaaccat ttgtataaat gtaatatgag atgagtattt
     2701 tagtttcaat attacctact atatttatca catttattac tattttgcaa cgctaaaatt
     2761 aataatagta ctatgtagga caaactaggt taataattat aactattggt tctaaaaggt
     2821 tttgtcatct caatgacaat atcataacaa cttttgcaaa caattttatt tttaaaagta
     2881 ttttttaata gcttatttcc atctttttca tgacaataga tgcataccgt atattcagta
     2941 ttttcattag aatttttcat attgtattat taataataat atgatatgct atttaaaaca
     3001 attattttat tttttgcatt tggtgtggta ctacttattt gcttaacaaa caaatgtagt
     3061 ttgtatctta tagtattata tcagtataac cttatactcc ctagttaatt aattagtata
     3121 ttgatctcag cttgaaattc aaattgaaat aaatggttag atttgagtaa tacattacac
     3181 tatcaatcaa ttcttcgttc actatctaaa taatatatta aacagctatt ccagggcttt
     3241 gaactacatt aacttattat cagatgaaat tattgtttct ataagttgtc taccgaactt
     3301 ttgatgtctg tttttcaaac ccttcaagat ttaatgagaa tacctgatac ttttcaaaat
     3361 gatataatta ctgctgtcat tgcattattt gtagtaattg atccaatagg aaatatacct
     3421 ttatttatag cattaacaaa aaaattagaa aaagaggagc aaaaaactgt atccaaaact
     3481 gctatactta ctgcaggaat attattgatt atttttggtg tagcaggctc acaaatttta
     3541 caactatttg gaataactat atttagcttt atgatagctg gaggtatact attatttata
     3601 actgcaatag aattactaac atacggtgaa tggagattcg caagtaatgt taaaggggaa
     3661 gcaggagttg ttcctattgc ttttccgtta ttggcaggcc ccggagcaat tactgctgta
     3721 ataattttat atcaaacttc tggattttta attactgttc tttcaattat tattgtaata
     3781 gcaattactt atgttatttt acgtatggtt aatccaattt acaaaatact tggtaataga
     3841 ggctcgatga tagtctcgag agtatttgct gttataatag cggcaattgc tgtagaatat
     3901 atagttgagg gtataaaaaa tctttttgta acttaacttg ccaaaatctc caacatcttg
     3961 ttaggaataa tatataatat accaattaaa ttgtagtcaa ttttattaat tttactagct
     4021 ttaattcatg aaaacaataa aataaaatcg cacaggagaa taatttagga tttggtctaa
     4081 acaatagcta atttagtttt taaatcatat taactcaaaa tgagtggtta gtcaaaaaat
     4141 tatggatgtt gttatggaat tttattattg ttatgctttt gttagtgatg aagtaaaatt
     4201 aacagagtca aatcttgatg attgatccgc attggacatg ttatcggcat tctcgattct
     4261 tgaagggaga tcagtattca gaatattatt agtactctta ctcggtaata ttttatctat
     4321 cacaatagtc aattttccat tatagtctcc aaattcgaca ttgagatttg ccctgccctg
     4381 ggttgcagat tgtgaagttt ttttccataa ttcgttccca tctgcatcat agataaaaat
     4441 atcataactt tgaattggga ctaccctctc taatgtagaa ggtacagaaa gacctgctga
     4501 actgaaagtg tcacccgaat tatttgattc taaattagga acactttgag gagtttgttt
     4561 aggtaaagtt gaatttaaaa agacaatttc tataggaaaa ttaaagggat cagaagtaag
     4621 agaggatttc aattgtacta acaaattacc tttttctgat ttattctcag agagaagaga
     4681 atccaatttc caattttcca gcttctcttg ttgtgaaaaa acttgttttc caataaatac
     4741 aggagctatg ctgcaaatta ataaaaatat aacaagagta tttatctgca tagtggatta
     4801 tattcgacat ttaatataag gatatcttta aacaaaaaag tattaataaa atagtacttc
     4861 actgacacaa aaaagattta aattaattga tacaatagcc gagtacacga tagttggtat
     4921 aaacgtctag aaatctcctt taattctatt atgtatgcag tttgaaatgc aaaataaaga
     4981 taaaatttat aataaagagc aaactattac tataaaactt atatgttttc tgacatttat
     5041 aaagaaaaaa ataactaatt acattatagt ataaaaaaaa tatatttatt ctagaattta
     5101 tttatctata tatatagtat aaaacacttc tattgtatat gtccaaagca gttgataaag
     5161 agattaaaca attatacctt tgtattgttg ttattgccgt ttccatagta tccatgggtt
     5221 cgtatcttaa tgttatagcc caagaaggaa gtattattaa gttatctgat atttctgata
     5281 atttcaaagc cctcctcctc aataatcagt ctcaaataag taatcaaact gcaatcgcaa
     5341 tagcatctct ccagaatgaa gaaaaaacac tttctgttaa tgcactggaa aatcttccac
     5401 gaggaagtac actcataaat actattgtta acgctccaat gaagatttat caattagagc
     5461 aaattggaag aatagttgaa aatggattaa tgattaacca aacttcttag agttttttat
     5521 tctaatttag atatatatca gattgttgaa tacttaattc gattagtaaa ttggttttgg
     5581 attattttac tcctttttta tgttttttat aagttactgt tctaaacgaa agaaaagtat
     5641 agtttgaatt acgtaatagc ctaatttata tttctaatat tcatttatgg ataaattttg
     5701 tttgatatct tttttgtttt ttttcttata ctaagtatat tggatagtct gaaactttga
     5761 ttatgattcc ttctattact tatattaaca tatcatattt tcgaggcatt taatatggac
     5821 gagtcgtgaa aaaaacaagt tcctcatatt aatatttgta tccttattgg tataatcatc
     5881 acatttgtaa ataaaagtat tgataacaca ttatttattt gaagagtttt tagtaatcag
     5941 ttaactacaa aagtaactct atattaataa aattatgagt gcagttagcg aaataatgtc
     6001 taagaaaagt gtagttagta tacaaacaaa ttctaacgta actgcattag atattactaa
     6061 attaatggta aaaaataaag taggttcagt tcttgtaatt gactccacaa attttcccat
     6121 tgggataatt acagaaagag atattataaa aaaggtttgt gtcacaaata ctttgcctaa
     6181 tgatatgaat ttagaaaaaa taatatcttc tccagtaatt acaataatgt cctatgattc
     6241 aattgaaact gcagcacaga aaatgaccga ttacaaaata aaacggcttc cggttttaga
     6301 agaaaatcaa cagattgtta gtatactaag tttgacagat ataactaaac atttatctaa
     6361 aattttgatc gatgattata aaagatttgg atttttgaag tctatttttg aatctgatta
     6421 aatatttttg attaaaccta cgtacgtata tttatccatg tcaattttta gaaaccacaa
     6481 tcaaggatcg agaaattaat acctaagtat tacaattcaa tttaactttt atttcttaac
     6541 tttattaata tcaaaatgca aatttatttt tttactatat gttttttaaa tttattaaaa
     6601 ttgaataagt gaattatttt ctcaattaaa tcattatatt atgtgcttga aacgcttttc
     6661 tattgctttt tcatctgcca tacctagcca aacatgtaaa ctagaggttt tacaaatggt
     6721 ctatgaggaa caaatcaata ttgtcaggtt catttaattt cggtcaaatc aaacca
//
LOCUS       DASVEV010000044        11314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_982785, whole genome shotgun sequence.
ACCESSION   DASVEV010000044 DASVEV010000000
VERSION     DASVEV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982785"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..642)
                     /locus_tag="VF222_02375"
     CDS             complement(349..642)
                     /locus_tag="VF222_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141303.1"
                     /translation="MPDPDIIRLLWFIFAGTRGGDNRIKIFDLLIKNPLNINKISEKL
                     QLDYKTIQHHMRILEKNNLVTKMGDKYGILYFISNYMEKNIESYYSIKNKLMP"
     gene            complement(777..1058)
                     /locus_tag="VF222_02380"
     CDS             complement(777..1058)
                     /locus_tag="VF222_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141304.1"
                     /translation="MTILMDINTIISIATIFILSVLLYLYSKIYLKTKATFTIGLMFF
                     AGMLVIQNLFVVYAYFAMAPLYAEGLLPYFLVVHIAELAGVASLLKITL"
     gene            complement(1083..2240)
                     /locus_tag="VF222_02385"
     CDS             complement(1083..2240)
                     /locus_tag="VF222_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7141305.1"
                     /translation="MNRDNINAVVMAIAVVSVIAGFAIYFNTQGSYISSKSTSDEFIP
                     LTGQINENGKVINIDKSQFKKAKDFAKISEYINTKPISLADLKDKVVLVDFWTYSCIN
                     CIRTIPHLNEWYDKYSDKGLVIIGIHTPEFDFEKNAENVKSAVQKFGIKYPILQDNDK
                     ETWNVYDNRYWPRKYLVDDEGYIRYDHIGEGAYQQTEKVIQALLSERAAHLGIKNINL
                     NDSQSVDDDSTLDYQDVNFSKIQSPELYFGYEFARTPLGNIEGLKPNQIVSYHIPDNS
                     EINLNSIYLKGKWKNNPDHMELQSNNGSILLKYNSKSVNIVAGGKGNFTVTQDGKLIT
                     NDLSKDLSNNGKFMIDGQRLYNLATNREYGEHTIEIDVKGKGFQIYTFTFG"
     gene            complement(2244..3062)
                     /locus_tag="VF222_02390"
     CDS             complement(2244..3062)
                     /locus_tag="VF222_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7141306.1"
                     /translation="MAEINILVSAIAGMGSFFSPCILPILPAFISYLSGITLHEIGDI
                     NKTKASVDNKNIESTITTTNNNNKKSISLQKSIRINIFLNTVYFVLGFSLVFAVLGVA
                     LNSILVNVGTSFQNTLQIIGGIVIISFGMFLILSSKINNLNFEKKLTRIPKFKTSYIT
                     SFVFGIAFAAGWTPCVGPILGSTLTLAATSPGEAYNLLLAYSLGLGTPFLITAAFFSR
                     ATGLIKKMVKYLKYFNLAMGTFLIILGILIFTNQLNILGNFPLANEIINLERGI"
     gene            complement(3476..3973)
                     /locus_tag="VF222_02395"
     CDS             complement(3476..3973)
                     /locus_tag="VF222_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141307.1"
                     /translation="MERIRILFTMLASIILLSVVSFSYMDIVDSVNAQSNTSSDPKQL
                     LRDFETAKETIQNKIQINFDQLINEIKIQLDFNSLDSYLSQASQDFINGKVSEGISEL
                     EKANRELKNSSMTIINAGDEIVSISKDNSTLMADGTKEILGHFGKILKDLGTNVDNSM
                     IQLKY"
     gene            4407..5588
                     /locus_tag="VF222_02400"
     CDS             4407..5588
                     /locus_tag="VF222_02400"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7141308.1"
                     /translation="MKRTYLLFLSALNLLLATFIITTFDQKVSLVNGQQLDTNNTEII
                     ISTPTKANATETAATKANATETAATKDSLIKPEANKDVKFCYDSQFGSLGTGDGQFNR
                     PHDIVFDSKGFLYVNDRELNNFQKFSPDGKFISQFGEKGEELGQFKSPYSMAIDSNDN
                     IYVLDRGNDRVQKVSTDGTPLGALYSYDGGFITSNDDIAKADKKDKKPSKEEKLKQFA
                     SPEAMAIDKEGNFYVTDTGHNRIIKFDKNFKYVSHYGQVGSGPGQFDHPHGIGVDSDG
                     NILINELNNARIQKFTNDGKFIKQWGSEGTGPGQFTLPLEHLKVDKDDNVFITDGANN
                     PRVQVFDNDGNFLTQFGKLGISNGEFQQPEHVAFNEKDDKGTVYVVDRGNARIQTFLP
                     C"
     gene            6319..6555
                     /locus_tag="VF222_02405"
     CDS             6319..6555
                     /locus_tag="VF222_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7141309.1"
                     /translation="MDTPTMEVGIINLKKDQEDTQQPHTSDEIYYVISGSGTIYIDGI
                     KNEVNSGKIIYIPKKIPHSFHAISNELIVLYILA"
     gene            6906..7577
                     /locus_tag="VF222_02410"
     CDS             6906..7577
                     /locus_tag="VF222_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141310.1"
                     /translation="MTIKNFKNMPLLLTIIFSFQIGLVVENNVYGIKYWCDENILVCN
                     GDEGDDQITGNDNNNIIYGWTGNDYLRGMAGDDVILGAEGSDTIIGDTSSDVFNINDH
                     GKDVLIGGAGDDILMGYNGSDDIYGGLGDDWLRDFGPRNSDELNNFYGEEGNDLLVGS
                     RSTDNFNCGEGIDVVLNFNSTQGDKSQSTCEIVIKENFNNSQNNQEYLAFAPLDNGTM
                     NLGKE"
     gene            8068..9735
                     /locus_tag="VF222_02415"
     CDS             8068..9735
                     /locus_tag="VF222_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141311.1"
                     /translation="MKYITLLFLSLFATSLPIISFSQMDYSAHPTITQQLPSTNTQSQ
                     PGYLILATIIINDNGGTQVASDFTVNILGKIVGKSFILASVQAVPAPEIKILSMQEGN
                     YSLSISNAQGYQVSQQNQCQGDIKPGEVKTCIITLNDDATATAPFPPQTGGGGEQTPP
                     LPTQCPAGQHFDQTIQECVTNIPPPPPTQCPAGQHFDQTIQECVTNIPPPPPPPPPTQ
                     CQAGQHLDQSTQQCVPNTPPPPPPTQCQAGQHLDQSTQQCVPNTPPQTDTPFHFTAAG
                     DFRDNTNTDENMAANNPEVVLILGDFSYNGNAAQWWSKNMDALNNLNVIGAVGNHDDP
                     NDDFLNLWPLNGGKWEFIKKVGNVAFVAFDTENNDPSTVDPLLAKAQADPTVDHIIPF
                     GHKTVFTPTPGNSLKPDADRGYFDVFKKYDKIRMILGGHNHFYARMNAVSGTDFIYVT
                     VGNGGANPHQDSGESGSPPKQYVRSNGALHCDVNNATISCKMVSNEGKVWDEFTITPG
                     NPPASDQKPAQAPEMLTQQLPSIIIQQQLPSIDMQSQPSYLLSNNYY"
     gene            10392..11018
                     /locus_tag="VF222_02420"
     CDS             10392..11018
                     /locus_tag="VF222_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141312.1"
                     /translation="MYQDISYEQLDINPETKNFEYSNGTLYYTYENSQIDFVFKYPAN
                     WKLLEFIDQFLSYDKVISFELKDSELFNQHKNKQIKNYFNDFYPYFPPLLSITLYDLK
                     FKNVSIKEFAKIKNEDLRNLFPDFKINLFTNNIKYIGRENIPAWKVEYSIEDAYTSNE
                     SSKDKRNGLNIWILRGDKVYEIEYIANSEGYFKNLREVTKLIDSIRFR"
BASE COUNT         4046 a         1690 c         1640 g         3938 t
ORIGIN      
        1 acatatgagt aattaattag atgtttatcc aatttataag taataataaa ttaaaaaaag
       61 caatacttaa tttatcagtc taaataactg ttgatggtat tattttattc taaattgtta
      121 ataattttct aatttattaa ataattgtat tgtatccaac atttctaaat cttccacaat
      181 tggaaagaga cggagcagat ttacaaacta agatctatca aatatagata ctcaatcaat
      241 ccctcataac gagagaaaat gacaagataa aaaagatgga atggctcatt tatctaatca
      301 ataattattt ttaagtgaaa gaatacaaca atgagaaaac tggatatatc aaggcatcaa
      361 tttgttcttt atactatagt atgattcaat atttttttcc atatagttag aaataaaata
      421 taaaatccca tacttatcgc ccattttagt tactaaatta tttttctcta aaatacgcat
      481 atgatgttga attgttttat aatctaattg taatttttct gaaattttat tgatatttaa
      541 agggtttttt attaatagat caaagatttt aatacgattg tcacctcctc tcgttcctgc
      601 gaaaataaac catagcaatc gaattatatc cggatcaggc attgaacatt atcattattc
      661 acaataattc ggagatgata aaaatcattt tattaccgca ggtatttcat ataagaaaaa
      721 ctttgtacta tatctaaaaa taatgtattc tatatataga taaatccttt aattattcaa
      781 agagtaatct tcaaaagact agcaacgcct gcaagctcag ctatatgaac tactaaaaaa
      841 tacggcaata gaccttcagc atataatggt gccatagcaa aatatgcata aacaacaaac
      901 aaattttgta taacaagcat gcctgcaaaa aacattaaac ctattgtaaa tgtagctttt
      961 gtttttaaat atatttttga atacaaatat aataaaacgc ttaaaataaa gatagttgct
     1021 atacttatga tagtatttat atccattaga attgtcataa taatatcctt tttttatttc
     1081 attcagccaa atgtaaatgt gtagatttga aatccttttc ctttaacatc aatttctatt
     1141 gtatgttcac catattctct atttgttgct aaattatata acctctgccc atctatcata
     1201 aattttccat tattagataa atctttgctt aaatcatttg taattaattt cccatcttgt
     1261 gttactgtaa aattcccttt tcccccagca actatattta cggattttga attgtatttt
     1321 aataatatac ttccgttatt gctctgtaat tccatgtgat ctggattatt cttccatttt
     1381 cctttgagat atattgagtt taagttaatt tcagaattat caggtatatg ataggataca
     1441 atctgattgg gctttaagcc ttctatattg cctaaaggag ttcttgcaaa ttcatatcca
     1501 aagtaaagtt ctggagattg tattttcgaa aaattaacat cttgataatc taacgtggaa
     1561 tcatcgtcaa cactttgaga atcattaaga tttatgtttt ttattcctaa atgtgctgct
     1621 ctttcagaga gaagagcttg aattactttt tctgtttgct gatatgctcc ttctcctata
     1681 tgatcatatc ttatataccc ttcatcatct actaaatatt ttcttggcca atatctatta
     1741 tcatatacat tccatgtttc cttatcgtta tcttgtaaaa ttggatattt tattccaaat
     1801 ttttgaacag ccgatttaac attttcagca tttttttcaa aatcaaattc aggagtatgt
     1861 atacctatta taaccaatcc tttatcagaa tatttatcgt accattcatt cagatgtggg
     1921 atagttctta tacaattaat acaactataa gtccaaaagt ctactagtac aactttatct
     1981 tttaaatcag caagggatat tggttttgta ttaatatatt ctgaaatttt agcaaaatct
     2041 tttgcttttt tgaattgtga tttgtcaatg ttgattactt tcccattctc gttaatttgt
     2101 cctgttaaag gtataaattc atcagaagtt gacttgctag atatatatga tccttgagta
     2161 ttaaaataaa ttgcgaatcc agctatcaca gatactactg caattgccat aacaactgca
     2221 ttgatattat ctctattcat tgatcatatg cccctttcta agttgatgat ttcatttgca
     2281 agtggaaaat tacctaaaat atttaactga ttggtgaata tcagtattcc aagaattatt
     2341 aaaaaggttc ccattgcaag attgaaatat ttcaaatatt ttaccatctt ttttatcaat
     2401 ccagttgccc ttgaaaaaaa agcggctgtt atcaagaaag gagttcctag tcccaatgaa
     2461 tatgctaata gtaagttata tgcttctcca ggagaagtag cagctaaagt aagtgtactt
     2521 cctaaaatag gacctacaca gggtgtccac cccgcagcaa aggcaattcc aaatacaaat
     2581 gaagttatat aactagtttt aaatttggga attctagtta attttttttc aaagtttaag
     2641 ttgtttatct ttgaagataa tatcagaaac attccaaatg atataatcac tattccacct
     2701 attatttgaa gagtgttttg aaaactagta cctacattta ccaaaatact gttgagggca
     2761 actcccagaa cggcaaacac taatgagaat cctaatacaa aataaacggt atttaagaaa
     2821 atattaattc taattgattt ttggagagat atagattttt tattgttgtt attggtagtg
     2881 gtgatggtag actcaatatt tttattatca acagaagcct ttgttttatt tatgtcacca
     2941 atttcatgta gagttattcc tgataaatag gaaataaaag caggtaggat gggtaatatg
     3001 caaggagaaa aaaatgatcc cattccagca atagctgaaa ctaatatatt aatttctgcc
     3061 atatatacaa tttagtattc ttaataaaaa aggatattcc caaattagac ccaaaaccta
     3121 cccaatttta tataaatagc ttacccagtt ttactgatga attacagatt caatggcttg
     3181 ctaaggctac aagttataag ctagtcgtcg ttagtatata ttaatcagac agaatctcag
     3241 aacttaaact ataatagact attgtaataa tatttgataa ttcctccaaa tattattttg
     3301 attttttatt catatctaaa aaaatttact atttatctat agtttggcat tctcctttat
     3361 taataaattc aaaatatgtt tttttcaatc tctggtgtat tattaataga tcttaatatt
     3421 ttatctacca tatgattttc agatataaca gttttattac tattttgaac atcaattaat
     3481 attttagttg gatcatagaa ttatctacgt tagttcccaa atctttaaga atttttccga
     3541 aatgtcctaa aatctctttt gtaccgtcag ccataagtgt tgagttatct tttgaaattg
     3601 aaacaatttc atcacctgca tttatgatag tcatactaga atttttcaat tctctattgg
     3661 ccttttctaa ttctgatatt ccttcagaca cttttccatt tatgaaatct tgggatgcct
     3721 gtgataaata agaatctaaa gaattaaaat ctagttgtat ttttatttca tttattaatt
     3781 gatcaaaatt tatttgaatc ttattttgaa tggtctcttt tgctgtttca aaatctctaa
     3841 gaagttgttt agggtcagac gaggtattag attgagcatt gactgaatcg acgatatcca
     3901 tataagaaaa ggagacaaca gaaagaagaa ttatcgaggc taacatagta aaaagaattc
     3961 ggattctttc catacattga gtgttattat actagtatat aattttgata tattgatcca
     4021 acccaaattc ataccaattg ttaggtattt tttattaatg aacctaaaag ttagatgtca
     4081 caataatcat taaatttctg attcttgaat catttgatat cgtaaatggg tattgatttt
     4141 taagtggtaa aattgaggtg aaaaagaata atataatata gaaaatttag aaaaatacac
     4201 atatggaata acaattttac atagatttta ttctctaata caattatgta actaatacat
     4261 taactgttaa tacaattatg taactaatac attaattgtt agtacaatta tgtaactaat
     4321 aagtcgatta ctatttaaat ataagaccaa tactttaata tcagacttag aaagttttat
     4381 atgttatttc aaatgattgt tatgttgtga aaagaactta tcttttattc ttatctgctt
     4441 taaatttatt gctagcgaca ttcataataa ctacatttga tcaaaaggtc tctctagtta
     4501 atgggcagca attagacact aataatactg aaattataat ttctactcct accaaagcta
     4561 atgctactga aactgctgca acaaaagcta atgctactga aactgctgca acaaaagata
     4621 gtcttataaa acctgaggcg aataaggatg tgaaattttg ttatgactct caatttggat
     4681 cattaggtac aggagatggc caatttaatc gacctcatga tattgtcttt gattcaaaag
     4741 gttttttata tgttaatgat cgagaactaa ataatttcca aaagttttca cctgatggta
     4801 aatttatttc ccagtttgga gagaaaggtg aagaattagg acaatttaaa agcccttata
     4861 gtatggcaat tgattcaaat gacaacattt atgttttaga cagaggaaat gatcgtgttc
     4921 aaaaagtatc tacagatgga actccattag gagcgttata ctcttatgat ggtgggttta
     4981 tcacaagcaa tgatgatata gcaaaagctg ataaaaaaga taaaaaacct tcaaaagaag
     5041 aaaaattgaa acaatttgct agtccagaag ctatggcaat tgacaaagaa ggaaactttt
     5101 atgtgacaga tacaggacat aatagaatta ttaaatttga taaaaacttt aaatatgttt
     5161 cacattacgg tcaggtggga tcaggaccgg gccaatttga tcatcctcac gggatcggag
     5221 tagattctga tggaaacatc ctcatcaacg agttaaataa tgcacgaatt caaaaattta
     5281 ctaatgacgg taaatttatc aaacaatggg gatccgaagg tactggacct ggccaattta
     5341 cattaccttt agaacattta aaagttgata aagatgacaa tgtgtttata actgatggag
     5401 caaacaatcc tcgtgttcaa gtatttgata atgatgggaa ctttctaacg caatttggta
     5461 aattaggcat aagtaatggt gaatttcagc aaccagaaca tgttgcattt aacgaaaaag
     5521 atgataaagg aactgtttat gttgtagaca gaggtaatgc aagaatccaa acctttctac
     5581 cttgttaaat aaatgataat attgtaaata taataaaaga tagagttaat caaatacaat
     5641 ttttttatat tgacactatt acttatatta taacttattg agtgtgtcgt aattcgttct
     5701 ctaattatga tatctagtat aatctactgc taagccatgg acatgtaaaa aattgtccat
     5761 gccatctcct ttctctcttc agcttgttat atatgtacca ttggtataat atttctgtat
     5821 atggtatttt tcaatccccg tattgttctc tatattatgt gacctctgtt gttcattatc
     5881 gtttgcataa tagaaccgat attaggtaaa attttattat ctgatcattt atagatacct
     5941 gtatggaagg agtgtataat tagatacgaa accagtattg ctgaccgtgg atgatgatcc
     6001 cctatgatat tgattccaaa aatggactca aatatcttga tatatcaaca atgaaataaa
     6061 ttttactatt attgataccg gttttttaga cataaaaaaa cgaaattcaa aatagcgata
     6121 taaagcattt tatcaaaatt atgaatcaaa tataaataat tttagtttaa gtccaaattc
     6181 tctgatactt ttattacaat ttaatagaaa aaattttata tgtatattat atttttttaa
     6241 tagatgcctc attctcaaat ttttgatata aataaatttt tgtcaaattt tcctaaatct
     6301 agatatttta ttggtataat ggatacgcca actatggaag ttgggataat taacttgaag
     6361 aaagatcaag aagacactca acaacctcac acctcagatg aaatttatta cgtcataagc
     6421 ggcagcggaa caatatatat agatggaatt aaaaatgaag ttaattctgg aaaaataatt
     6481 tatattccaa aaaaaatccc tcactcgttt catgctatta gtaatgaatt aattgtttta
     6541 tacatattag catgaatatc taattaggac caaattaatg tggttattct cattcttctc
     6601 aaatagtaac ctcttgtaac atgtgattta gttacatata caaatagcaa agatacatta
     6661 aattcttcta actttgttct attttttcta tttatttttt ggttcacctt ttttttattt
     6721 ttcgagtgca tccaataaga gaactaatat cacaaggggg caagtgggat gtggaacaaa
     6781 tttgacctat tatttggtcc ttcattttaa aatatattac atatcggaag tatatactct
     6841 tataaaaaaa tatgatgttt attatataga aaatcttaat agtttactca tctaaagaat
     6901 agaaaatgac tataaaaaat tttaaaaata tgccattatt attaacaata attttttcat
     6961 tccaaattgg actagtagta gaaaataatg tatatgggat aaaatactgg tgtgatgaaa
     7021 atattttagt ctgtaatggt gatgaaggag atgaccaaat tacaggaaat gacaataata
     7081 atataattta tggatggact ggaaatgatt atttacgagg tatggcaggc gacgatgtta
     7141 ttttaggtgc tgaaggttcg gatacaatta ttggcgatac atccagtgat gtatttaata
     7201 ttaatgatca tggaaaagat gtcttgatag gaggagcagg tgatgacatt ttaatgggct
     7261 ataatggtag tgatgatatt tatggtggtc tcggggatga ttggcttcgc gatttcggtc
     7321 caagaaattc agatgaacta aacaattttt atggtgaaga aggtaacgat ttgctcgttg
     7381 gtagtaggag tacagacaac tttaattgtg gagaaggaat agatgtagtt ttgaatttca
     7441 attcaactca aggagataaa tctcaatcta cttgtgaaat agtaattaaa gaaaatttca
     7501 ataatagcca aaacaatcaa gaatatctag cctttgcacc attagacaat ggcaccatga
     7561 atttgggaaa agaataaaca aatatttgac tgtatgaacg gctcttttca agatttttga
     7621 gaaaagaaat tattttccaa atttataaga aagataagta tctcctttta gattcaactg
     7681 cataaacaaa taattagtat gcattagatt catcaaacct ttcttaaatt attttcaatt
     7741 ctatattgat ttaccttaat tttttatttt taactcaatc caatttgaaa actaatatca
     7801 caatagagcc agtgggattg actcccctat tgtatagcgg tttagataca aggacctcct
     7861 atatatttat aagaggggtt aaatctattt tcttaattta acacatctga tattctaata
     7921 tattttatat aaatatcaac tatagaaaag aaattaaagt tttttaatat aaataataat
     7981 attaattatt cagaataatt cttatttgat tatgtaaccc agcccaaata tacatttgaa
     8041 ttacttttaa aagtggattt aataactatg aaatatatca cgttgctttt tttatcattg
     8101 tttgctactt cattaccaat tattagtttt tcgcaaatgg actattctgc acatcctaca
     8161 attactcaac agctaccatc aacaaatacg caatcacaac caggttatct tatcttagca
     8221 acaattatta ttaatgataa tggaggtaca caagtcgcat cagattttac agtaaacata
     8281 ttaggaaaga tagtaggaaa atcatttatt cttgcttcag ttcaagctgt tccagctcct
     8341 gaaatcaaaa ttctttctat gcaagagggt aattattcac tatctataag taatgcccaa
     8401 ggatatcaag tctcccaaca aaatcaatgc caaggagaca ttaaacctgg agaggttaaa
     8461 acttgtataa ttactctcaa tgatgacgca acagcaacag caccattccc accacaaact
     8521 ggtggaggag gagagcagac gccaccacta ccaactcaat gtccagcggg tcagcacttt
     8581 gaccaaacaa tacaggaatg tgttacaaat ataccaccac caccaccaac tcaatgtcca
     8641 gcgggtcagc actttgacca aacaatacag gaatgtgtta caaatatacc accaccacca
     8701 ccaccaccac caccaactca atgtcaagcg ggtcagcact tagaccaatc aacacaacaa
     8761 tgtgttccaa atacaccacc accaccacca ccaactcaat gtcaagcggg tcagcactta
     8821 gaccaatcaa cacaacaatg tgttccaaat acaccacctc aaactgacac ccccttccac
     8881 tttacagcag ctggtgactt tagagataat actaacactg atgaaaacat ggcagcaaac
     8941 aatccagagg ttgttttgat acttggtgat ttttcatata atggaaatgc tgcacagtgg
     9001 tggtctaaaa acatggatgc tcttaacaat ctcaatgtta ttggagctgt tggtaatcat
     9061 gatgacccaa atgatgattt ccttaattta tggccactca atggaggtaa atgggaattt
     9121 attaagaaag taggtaatgt tgcttttgtt gcttttgaca ccgaaaacaa tgatccatca
     9181 accgttgatc cattattagc aaaagcacag gcagacccaa ctgtagacca cattattcct
     9241 tttggtcaca agacagtatt cactccaact ccaggaaact cattgaaacc agatgctgat
     9301 agaggatatt tcgatgtgtt taaaaaatat gataaaatca gaatgatatt gggagggcat
     9361 aaccactttt acgctagaat gaatgcagtt tcaggaacag atttcattta tgtgactgtt
     9421 ggaaatggag gagctaatcc acaccaagat tcaggtgaat ctggttctcc accaaaacaa
     9481 tacgtcagaa gtaatggagc tttacattgt gatgttaata acgcaacaat aagctgtaag
     9541 atggtatcaa atgaaggaaa ggtatgggat gaatttacca taacccctgg taatcctccg
     9601 gcatcagatc aaaagccagc tcaagcccca gaaatgctta cacaacaatt accatcaata
     9661 atcatacaac aacaactacc atcaatagac atgcaatcac agccaagtta tcttcttagc
     9721 aacaattatt attaatgata gtggtggcac aaaagttgca taagatttta caatcgatat
     9781 agatgactaa ttcttgacct tttttggtta ttttatgctc taccattgga ggaattcatt
     9841 tcatgatttg tttgctggta tatattagaa caattggaag aaaatttata aaaattatgc
     9901 tacaaactat ttccggtatt attattaatg tagtcaacat atccacattc ataaagatat
     9961 tagacgattc ttaggacaga tatatttctg aaatatggtt tgagttcaat taaggtaaaa
    10021 aagaaaactt tcttttaact agaaaatgaa taattgtttt attattctgg ctagtaacaa
    10081 agaattgttt ccactttctt gaaaaaagtt accttcatct tgaataaaat tattgtttaa
    10141 tcatcaaaaa accttgtcat tcctatctat ttgaaatatt gtttactagt tatacaattt
    10201 tttcgaatga caaaatatag cttcaatcag acagtctaat ttatgaagat aaatatatag
    10261 aattctttgt tttaattata tatgtaagag atataatatc ttgagttatg aaaggtccta
    10321 gatttgaggg tagaatgtgt tatcagttct taatcactct tatatgttta ttattcttaa
    10381 tcagttttta tatgtatcaa gatatatcat atgagcaatt agacataaat cctgagacta
    10441 aaaattttga atactcgaat ggaacacttt actataccta tgaaaattcc caaattgatt
    10501 ttgtttttaa atatccagct aattggaaat tattagaatt tattgatcaa tttctgtctt
    10561 atgataaggt aatctctttc gaattaaaag attctgaact tttcaatcaa cacaaaaata
    10621 aacaaatcaa aaattatttt aatgattttt atccatactt tccccctctt ctgagtatca
    10681 cattatatga cttgaaattt aagaatgttt caatcaaaga atttgccaag ataaaaaatg
    10741 aagatttacg aaatcttttt ccggatttca aaataaatct ttttactaat aatataaaat
    10801 atattggaag ggaaaatatt cctgcatgga aggtcgaata ttcaatagaa gatgcctata
    10861 cctcaaatga atcatctaaa gataaaagga atggtttaaa tatttggatt ttgaggggtg
    10921 ataaggttta tgaaatagaa tacattgcta atagtgaagg atactttaag aacctcagag
    10981 aggtaacaaa attaattgat tctattagat ttcggtgatc ctcatatatt aatcatataa
    11041 atcatattaa ttacaattga aaaactgaaa atttgtatca tctgatgagc ttctgctgta
    11101 tcaatgcaat attataaata gtattttagg tttatagagt ttcgaaaatg ataaaccttg
    11161 cattagtttt ggtgattaat atttttgctg ctagtatcct atcaatgttg acaaatggga
    11221 cctttggttt ttttgcactg aattatgcat attcagttga agtaacttct gattccttaa
    11281 aagaaattcg agaaaacctt cttgaagtta gact
//
LOCUS       DASVEV010000045        12600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_983139, whole genome shotgun sequence.
ACCESSION   DASVEV010000045 DASVEV010000000
VERSION     DASVEV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983139"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..871
                     /locus_tag="VF222_02425"
     CDS             392..871
                     /locus_tag="VF222_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141313.1"
                     /translation="MYKSTYNFGLFLMASLILLSIIAAIPMSNMNLFSNVLASGKITD
                     KNYNSLDSHSPAGITFLNSTNVYQVTNGSVIPNGLNGGAAAFCDEGDFLINGGYSIET
                     PEQFASNINHLAFDPYDAQGSNFSNALHIFLAYSNATSTSANVTIIADCFDNPPLRP"
     gene            1421..1858
                     /locus_tag="VF222_02430"
     CDS             1421..1858
                     /locus_tag="VF222_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141314.1"
                     /translation="MAKQQQLLNQESPVSEVLNRFKKELTKNIERQIPVPNFRIVSEE
                     SKQEASISQIIRMYGKQFKQIRKQYTDGLGGRCVMGLIMSYYGWDGKCITNAERKLYA
                     ALVALQRAGISKELLIELNDSGATFDDIADYLDNNFGLASSNR"
     gene            1939..2094
                     /locus_tag="VF222_02435"
     CDS             1939..2094
                     /locus_tag="VF222_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141315.1"
                     /translation="MSYRTSFSKMLGVEKYLWLSIVVNVKGDKKSLIITVNLIVLKFP
                     HHSTEPV"
     gene            complement(2416..2583)
                     /locus_tag="VF222_02440"
     CDS             complement(2416..2583)
                     /locus_tag="VF222_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141316.1"
                     /translation="MRAKEFDTNGTFITKFGSLGKRDGQFTDTEYLALDSDGNIYVSI
                     KKPILSQSSSM"
     gene            complement(2717..3067)
                     /locus_tag="VF222_02445"
     CDS             complement(2717..3067)
                     /locus_tag="VF222_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141317.1"
                     /translation="MLSSTTIVIVLVMSTIISLSIFTMTNSNVFAQQNKFTEELNLLN
                     QSITALENQDKNAKKTLFNIEGIMEEKLKDNPEAVNAEKRVEAAIKMISEDNFQAALD
                     HTKEAIKSLSNLNN"
     gene            3175..3627
                     /locus_tag="VF222_02450"
     CDS             3175..3627
                     /locus_tag="VF222_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141318.1"
                     /translation="MNFQLILVYILAAVIISTSIIYFILASNEYVHLIEFASEGLEGE
                     IPELQIEIALFAGSAIIYLGLLGWVLVKKLKSILPYSILIIVSTILIITYAASRTMGV
                     PVIGVEFYIGKYDMITKVLQGIIIAISGYLIYRIKTGNRQQQNIMKKT"
     gene            3822..4031
                     /locus_tag="VF222_02455"
     CDS             3822..4031
                     /locus_tag="VF222_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141319.1"
                     /translation="MTKINGIKQQRPDRISLILNKLEQNGYIKSIDTSNAKFYLITEK
                     GFDAYLKWIKDFLTFVRSMNNENID"
     gene            complement(4028..4861)
                     /locus_tag="VF222_02460"
     CDS             complement(4028..4861)
                     /locus_tag="VF222_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141320.1"
                     /translation="MKNNEPRIGKIDANINSDSPLLLTQYSSKASQLIKSFTRNILFV
                     ILILFVIATMTIFDLLTDLGFLTVLPDETIDSMIIVVSLFLIIFTILIVRPVFRSQRI
                     LDKWANLFDKNSIRTGIILSINNKSKEEILVALSENIEQIAIPLQNYLSKSDNKEFYD
                     VSIDDTTFDILIDKSKIKPIDSDSLKNTIHDYGSILIKITEGVIDKNLTRTFIQSLQK
                     YRKQQGNKIGLAMIIGESITQESYDLINKIKDKIINENLILIEKPTTDSDLMNLNNML
                     T"
     gene            5919..6896
                     /locus_tag="VF222_02465"
     CDS             5919..6896
                     /locus_tag="VF222_02465"
                     /inference="COORDINATES: protein motif:HMM:NF017121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HEX7141321.1"
                     /translation="MKNWSTYKDLLARHRSHPPFTSNLLNILNKKLTIVSSRNHFNLE
                     TGQLNMSHNIEDYDSFNVPGLNGNPCSGNNELAVYIHGVWTRQIAAKEQIDRTNLSLI
                     SNEYNIPVVGFSWDSNTVINPSGWNIAKSIAKQNGPKLAKFLSDFRTNCPNHNIRIIA
                     HSLGAKVVESALLGLNNNNTWKNNSAYNITSIHLIGAAISDRATSKNTQFGSAIENTV
                     NNFYNLYNPKDNLLRSAYVKTEKENPLGLFGLNKAEPFPSNYTERNVILEIPPFKKAS
                     GIYKPFVDNTVSEWGNNHSGYIGFRGVDGKLKNDGAINVIVADWKTNKR"
     gene            7308..8234
                     /locus_tag="VF222_02470"
     CDS             7308..8234
                     /locus_tag="VF222_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141322.1"
                     /translation="MSFKWKNDKFILGLRLFSILVLLSTLLFYNENISITAQNDKKDD
                     TNKNDTNNVDFTAIKTKINLNNINIQNYDRLKLVGYLNGEGQTIYINLKEIKDKINLK
                     DNSLTVNLKFNKSNDVSKVMFDDEYYACGYMIDGKLNVANTDSNLTLYDCDEGNISLT
                     STDKDTVKLFYTLKKFSKSNALYKTNNPSTTKETPKEVKLRIDVPIHDNKKINDMYVV
                     AMIKGEYQIKKIDAQNGIGKGDKKSISERLSIPFTFDRNTEVGLIEPGDMFFGCVTSD
                     EFPDQNSDCEKRIITDLSKIGQVCARLDSACK"
     gene            8939..10003
                     /locus_tag="VF222_02475"
     CDS             8939..10003
                     /locus_tag="VF222_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141323.1"
                     /translation="MVLTHFSINFVLFAEDGFFVVPEDQFFNPEDYGRKSALSPGGEN
                     YSEQFKSEDELYNTNPKQLTDSINLTDFNFALAGDWGCTKNTAKTVDLIQSHKPELVF
                     SLGDTSYGKDINCWVSIVKPISDRMKAVIGNHDVMSPTLLDQHLKQFGLDKSYYSFDY
                     NNIHILMMDTESSYLPGSDPDFSDLENTDHYRFVENDLSIASKNPAIKWIIVMSHRQL
                     YSSLCGDHDSCEPIKKLRDAYHPLFEKYGVDLLFSGHAHNYQRTYPLFYNDINSSQPL
                     IEEEDKIEYTSSKGMVQIIVGTGGIDFDNFSNQEPFVVYQEDSQYGFLNVDVIDQGNV
                     LLGRYYANTGEVLDEFKIVK"
     gene            complement(10436..11062)
                     /locus_tag="VF222_02480"
     CDS             complement(10436..11062)
                     /locus_tag="VF222_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141324.1"
                     /translation="MFNLFDKYKLHIVNLKASFLVSIIIFFMAESGISIIFSDCCPIV
                     LVSADINNNNNSSLSSILNNYTYTFVEQKTVMSQPAPVKHQGQPSHEVVFALPLRDDG
                     KVWSGTVTFTASKPIEVEILHTYAPEQNLDSLHGHPYHALLPGNKSIAITHLRDIVDV
                     PIEINGTGISSSSFNFVGNALVFHKTNGEPFTVTYTIDAKVKDIGINN"
     gene            complement(11190..11810)
                     /locus_tag="VF222_02485"
     CDS             complement(11190..11810)
                     /locus_tag="VF222_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141325.1"
                     /translation="MELHHQIILIIFINVIFTLLIFSPDKFIYALPDYDITQNQNLEN
                     KLNFKLYDITLENTFYNSKLLSFKLPTGWGITHENEQLDFLHLDLYPKNGDQAQILIV
                     SSNSMLLPGVEELLPSMVESMFKQSAGDDKDIVKIRSFDIGYVIDGTEMTTDLYKVLL
                     GNNSVMTLVVYGLIDSDSMFIAYFMSPQYDKYLPEFELLMNNINFK"
BASE COUNT         4637 a         1724 c         1790 g         4449 t
ORIGIN      
        1 taacacacca tctaaattta tcatcctaca aaataatgaa acatagtttt gtttttaacc
       61 tttttcggcg ccgcaaattc caaagtcaaa attgttttat cataaaagct tttttatata
      121 ataataagca gagcataaca tgtctttaaa agatacaaaa attactaaaa ccagaactaa
      181 cttttcagta taaaggtaca gatatcaaac taatttctca gtatagttac agaaaattaa
      241 ttagtactta atatgataat atgtttagaa atgaattagt aagaaatatt cgaaaatgta
      301 taataaataa taattctcta tctaaacaat agtatatgtt actgaatgaa tctcccaatt
      361 ctaaatataa ttagaaagtt agttaacaaa tatgtataag tcaacataca attttggcct
      421 ttttttaatg gcttcattga tattattatc aattatagca gcaataccaa tgagtaacat
      481 gaatctcttt tcaaatgtat tggcatcagg aaaaattact gacaagaatt acaactcact
      541 agattcacac agcccagcag gaattacttt cttgaatagt acgaacgtat atcaagttac
      601 taatggtagt gttataccta acggtctaaa tggtggtgct gctgcttttt gtgacgaagg
      661 cgattttttg attaatggag gttatagtat tgaaactccc gaacagtttg ctagtaatat
      721 caatcatttg gcatttgacc cctatgatgc acagggctcg aacttctcca atgcattgca
      781 catatttctt gcatattcaa atgcaactag tacttccgca aatgtgacaa ttattgcgga
      841 ttgctttgac aatccaccat tgagacctta acttcctcta tttttaattt gtgaaatctt
      901 ttaaaaatac atactaaaat acattaaaaa taaatcttaa atattatact ctctttattc
      961 ccaaataata aactcttatt ttagaaccag tatcggtatt attctttata tattccttat
     1021 atggacaatt ttatttgcat atctaagttt ctatattctt atttttctta gtacttatta
     1081 tcggaaaacc aaaaagtaaa agtagtaaac caatcattgg aacaaatatt cgaatagaat
     1141 tctcggttat agttatgatt aatggaatta ttaacatgat agcgcctaaa ccataaaaat
     1201 tattcatatt cttgtctgtt tcaataaatt tctttatttc acatattcta ttaaacaata
     1261 tccatttgtt ataaaagaaa agaattgtat cgaactttcc acagccttca ccgaaatata
     1321 gtttcctaca caacgtctaa aaggcgacca aatacccttt cggaagaaac ccattacata
     1381 gcttctaaat agtttgtttg tgattattta ttggcaatat atggctaagc aacaacaatt
     1441 attaaatcaa gaatcacctg tttctgaagt tctaaataga tttaaaaagg aattaacaaa
     1501 gaacatagag cgacaaatcc ccgttcccaa ttttagaatt gttagtgaag agtcaaaaca
     1561 agaagcatcc atttctcaaa taatccggat gtatggaaaa caatttaagc agattagaaa
     1621 gcaatatacc gacggacttg gtggaagatg tgttatgggt ctcatcatgt cttattatgg
     1681 gtgggatggc aaatgtatca ctaatgctga aagaaaatta tatgctgcat tagtcgcatt
     1741 gcaacgcgcc ggtataagta aagaattact gatagagttg aatgattctg gtgcgacatt
     1801 cgacgatata gctgattacc tggacaacaa ttttggattg gctagtagca acaggtaatc
     1861 gtctaactca tcaaatggcg gtctttgatg agtgtgaaat ttttatacga ttacaccgaa
     1921 ttcatgaatt aattaaattt gtcgtatcga acatcattct ctaaaatgtt aggcgtcgaa
     1981 aaatatctat ggcttagtat tgttgttaat gtaaaaggag ataaaaaatc tttaattatt
     2041 acagtaaatc tgattgtatt gaaatttcca catcattcca ccgaacccgt atgaaattat
     2101 acattaatag aagatgataa agaggggttt atgctatggt cttcaatatt gttgtacccg
     2161 tcttcctaga ttacaaaaat ctatcgtcca tagatgacac gtaatttaat gaaagtagaa
     2221 aagataatcc tattaaagta aaaaaagtaa ttaaaacaat tttattttta taaaacattt
     2281 tagagaaaga atttgttcaa gcctttaaaa tctatatcaa atattttcaa attaggatat
     2341 gatatatttt gttgtttgtt atagataatt attgtaaaat aagaaataaa cataaaaaaa
     2401 atatgtgata ctatcctaca tacttgaaga ctgtgatagt attggctttt taatagacac
     2461 ataaatattt ccatctgaat ctagtgcaag gtattctgtg tctgtgaatt gaccatcacg
     2521 tttccctaga gaaccaaatt tcgttataaa ggtcccattg gtatcgaatt ccttcgctct
     2581 cattttccta tgtgaaattc gctaattata tattttgtag tatattgtta aaatgtggta
     2641 tttatgtaat caaaactaat gctgtagtag tttatgaaat aataaaaact aatgttaccg
     2701 acatgagtac tttaaactaa ttattcaaat tactcaaact tttaatagct tcttttgtat
     2761 ggtcaagtgc agcttgaaag ttgtcttcac ttatcatttt aattgcagct tctactcgtt
     2821 tttctgcatt tacagcttct ggattatctt ttagtttttc ttccattata ccttcaatat
     2881 taaatagtgt cttttttgca tttttatctt gattttctaa tgcagttata ctttgattta
     2941 gtagatttaa ttcctctgta aatttattct gttgagcaaa tacatttgaa tttgtcattg
     3001 taaaaattga cagactaatt atagttgaca ttacgagcac aattactatt gttgtagatg
     3061 ataacataat tatcagcaaa atcaaattat ataaaaaggg taatgtaact tagttaacaa
     3121 tgttcagaat attgttatat caatattttt attttattaa actgactcaa cagaatgaat
     3181 tttcagctta ttcttgtata tatacttgcg gcagtaatca tatctacttc aataatatat
     3241 tttatcttag catcgaacga atatgtacat ttaatagagt ttgcttcaga aggattagaa
     3301 ggtgaaatac cagaacttca aattgaaatt gcattgtttg caggctcagc tataatttat
     3361 ttaggtctat taggatgggt tttagttaaa aaattaaaaa gcatattgcc ttattcaatc
     3421 ctaataatcg tttctacgat actaataatt acatatgctg cctctagaac gatgggagtt
     3481 cctgttatag gtgtagaatt ctatattggt aaatatgaca tgataacaaa agtattacaa
     3541 ggaattataa ttgctatatc ggggtatttg atatatagaa ttaaaacagg caatagacag
     3601 cagcagaata ttatgaaaaa aacataaacc atatctgata attcaataac atttaaaaaa
     3661 tctataatat tttttaaaaa taacatgcct tatttacttt aaaaaattag atataatgat
     3721 tggaaaaagc aaagagattc tcatcggaat atactatgtt atccataata gagtatcttt
     3781 gcctatattc aagaaaaaca ccaattagca aatatcacat aatgacaaag attaatggaa
     3841 ttaaacaaca acgtcccgat agaatttcct tgattttgaa taaactagaa caaaacggtt
     3901 atatcaaatc aatcgatacg tctaatgcaa aattttatct aattacagaa aaaggatttg
     3961 atgcttattt aaaatggatt aaagattttt taacttttgt cagaagtatg aataacgaaa
     4021 atatagatta agttaacata ttatttaaat tcattaaatc agaatctgta gtaggctttt
     4081 ctattagtat tagattttca ttaataattt tatctttgat tttattaatt aaatcatagc
     4141 tctcctgagt aatactttct ccaattatca tggctaatcc aattttgttt ccttgttgtt
     4201 ttctatactt ttgcaatgat tgtataaaag ttcgagtaag atttttatca ataacaccct
     4261 ctgtaatttt aattaatata cttccataat catgaattgt attttttaag gaatctgaat
     4321 ctataggttt aattttcgat ttatcgatta agatatcaaa agttgtatcg tcaatactaa
     4381 catcataaaa ctccttatta tcagattttg aaagatagtt ttgtaatggt attgcaatct
     4441 gttcgatatt ttctgataat gcgaccaaga tttcttcttt actcttattg ttgattgata
     4501 atattatacc agttctaata gagtttttat caaagagatt tgcccattta tctaatattc
     4561 tttgtgacct aaacacaggc cttactatta aaatagtaaa tataataaga aataaagata
     4621 caacaattat cattgaatca attgtttcgt caggtaaaac agtaagaaac cctaaatccg
     4681 taagtaaatc aaaaattgtc attgttgcaa ttacaaatag aattagtata acgaatagaa
     4741 tattccgtgt aaatgattta attaattgtg atgccttaga actatattgt gtaagtaaga
     4801 ggggagagtc gctgtttatg ttagcatcaa ttttacctat cctaggctca ttattcttca
     4861 ttgtatataa taaattttag gtgtctaaaa tatataaaca ctgatataac tatattctag
     4921 aatagatata attaatcaaa aaaatgttat attatgacga atcctcaatt taaaattcta
     4981 aattttttac ttgcttaaca ttaaatctga tttattataa aaatattaaa aacaataacg
     5041 agaattagcc catgatacta aattttgcaa ttaatggttt tggaagagaa gagggagttc
     5101 ccttcgtgtt tgtcttaaaa acaaagaatt tatgagtatg ataatctagt tgcgataaag
     5161 atttgacaaa tctaataaat cttgtatgac aatgaagaat gaagatttgt ttgcagattt
     5221 gttgacttaa tacagtttat ctattgaatc atattattaa tataaaaagt caaattaatg
     5281 gattgaatat acgttgacaa ttctgaccta gcagtaatac aaaaatttca attttagaaa
     5341 aataataaat ctttaatttt atttttaaat caattcaaat aaatttttta aaaattttaa
     5401 gtgtaattat ttaaatgggt attcctccct ccgcaaattt ccaactaatt cacatacatt
     5461 aatatttcaa caaacaactt aaaaaataag tttctaataa taaaagctaa atacaatgta
     5521 gtaaaaccct actacatgga cgaatccact cctgcacaac ttttcttaat ataaaaagaa
     5581 cccatttggt aaaatctaat tccaatttaa gcaaatttct ctttccatat tgctgttata
     5641 ctcacttcaa gagcatcttt atcatctttg gtaaattcta aaatagcttc tgttgtatgc
     5701 ctattgtagc tgcccgaatt ttctaatata atctaaaaat gcttgccttt agttttcata
     5761 tgttagaaca ttatctgcag caactactga tttttattga gcaaaccatt tttcttccac
     5821 aaattcaatc aaaagttgca tttaaaggac aaaactgaga agtatttttg ctttataatt
     5881 gtagaaagat atttaatgaa tattgcaaat tacaacgtat gaaaaattgg tctacttata
     5941 aggacttact agcgagacat cgtagtcatc caccttttac atctaatctt ttgaatatat
     6001 tgaataagaa attaacaata gtatcgtctc gcaatcactt taaccttgag acaggccaac
     6061 taaatatgtc tcataatata gaagattatg actcttttaa tgttcctgga ttaaatggaa
     6121 atccttgctc tggcaataat gaattagcag tatacattca tggagtatgg acaagacaga
     6181 ttgctgctaa agaacaaata gatagaacca atttatcatt gatttcgaat gaatataata
     6241 tcccagtagt aggttttagc tgggattcaa atactgttat aaatccttca ggatggaata
     6301 ttgctaaatc tatagcaaaa cagaatggac caaagctagc aaaattcctg tctgatttta
     6361 gaaccaattg tccaaatcat aatattagaa taattgctca ttctttaggt gcaaaagtag
     6421 ttgaaagtgc cttgctagga ttaaataata acaatacatg gaagaacaat tcagcatata
     6481 atattacttc tatccatctt ataggagcag ccattagcga tagagcaaca tcaaaaaata
     6541 cacaatttgg atctgccatt gaaaatacag taaacaactt ctataatcta tataatccta
     6601 aagacaattt acttcgaagt gcatacgtaa agacagaaaa ggaaaatcca ttgggcttat
     6661 ttggcctaaa caaagcagaa ccatttcctt caaattatac tgaacggaat gttatactcg
     6721 aaattccccc atttaaaaag gctagtggaa tatacaagcc atttgttgat aacacagtat
     6781 ctgaatgggg aaataaccat tctggatata ttggcttcag aggtgttgat ggtaagttaa
     6841 agaatgacgg agctattaac gtaattgtag cagattggaa gactaacaag agatagataa
     6901 ggcaaaacaa gcagaaacaa taaagatgaa atagcttatg tttattccaa agataaatta
     6961 actgaattga attaacatct taaaataaaa gatttcaaaa ataaaaaata acaatgataa
     7021 tattaacaat caatagtaaa atagaattgg aatgaaattt ctataggatt tcaccgaacc
     7081 agtgattaaa ttattggtga cagtaacaaa acaatatgaa atgaaattgt ttattgtatt
     7141 ggttaaatca caacaatttt aatatagtat cctcaaatct catttttaat ttaaaaccaa
     7201 actatacaag aatttatgta gatatataaa ggttaaattt tatttactat tacccatagt
     7261 gaatttaatc atattgttta tattattttt ataacaatct tttatcattg agttttaaat
     7321 ggaagaatga taaatttatt ttaggattac ggttattttc tattttagta ttattatcta
     7381 ccttactatt ttataatgag aatatttcta taactgctca gaatgataaa aaagatgata
     7441 ctaataaaaa tgatacgaac aatgttgatt ttactgctat taagacaaaa ataaatttga
     7501 ataatattaa tattcaaaac tacgatcgct taaaattagt gggttatctt aatggtgaag
     7561 gacagactat ttatattaat ttaaaagaga ttaaagacaa aataaattta aaagataatt
     7621 ctttgactgt taatcttaaa tttaataaaa gtaatgatgt ttccaaagtc atgtttgatg
     7681 atgaatatta tgcatgtggg tatatgatag atggtaaatt gaatgttgct aacacagatt
     7741 caaatcttac attatatgat tgtgatgaag ggaatattag tttaactagt actgataaag
     7801 atacagttaa actattctat acacttaaaa agtttagtaa atcaaatgca ctttacaaga
     7861 ctaataatcc atctactaca aaagaaactc ctaaagaagt aaaattaaga attgatgtac
     7921 ctatacatga taacaaaaag ataaatgata tgtatgtggt agcaatgata aaaggtgaat
     7981 atcaaattaa gaaaattgat gcacaaaatg ggataggaaa aggagataaa aaaagtatta
     8041 gtgaaagact ttcaattccc tttacttttg atagaaatac agaagttggt cttatagaac
     8101 cgggagatat gttttttgga tgtgttactt ctgatgaatt tcctgatcaa aattcagatt
     8161 gtgaaaaaag aataataaca gatttgtcta agataggaca agtatgtgca agattagata
     8221 gtgcttgtaa ataataaaat gaaaactcaa cccattcaaa catttgtatt aatatttaaa
     8281 ttcaatccac cttctataat ttgatagtat attataaatt aattagtcca tagtattttt
     8341 gaagctgtac tgataataaa ctaagtgatg aggatatttg tgataatcta tgttaatcat
     8401 aagctaatga agatatatct ctattaaatt aatttgtgtt attaagatat actgctattt
     8461 caattactaa tattttatgc ttattggaat catctgtagg cgtttattca ctatatactc
     8521 tattataata tactatacaa agcctataag gaaagattag gggttcctac attaggtgca
     8581 acattatgtt tcatacttga tttgcaatta tacgcttacc aaaaaatcat agtcatcgcc
     8641 tattactgga attttaatat aattttttcc tctctgtaac tctagcagct ttatattgta
     8701 tatcctttat caaacatttc tatattttag aatatggtaa tatttgctca acatatctgt
     8761 tgaattaagt tattgaaaat aaagaaaata tatgataagt aaaaaatgtt atggaagaaa
     8821 agaccataag atgatttaat aacattcaac caaatcttca aaaagggttt caatctattt
     8881 ttatataaga atattatcta tgtatgctct cttattgatt tttcttttaa ttttattaat
     8941 ggtacttaca cacttctcca taaattttgt tctctttgca gaagatggat tttttgttgt
     9001 ccctgaagat cagtttttca atcctgaaga ttatggcagg aaatccgctt tatctcctgg
     9061 aggagaaaac tattcggagc aatttaaaag tgaagatgaa ttgtataata caaaccctaa
     9121 acaattgaca gattcaataa atctgactga ttttaatttt gcacttgctg gagattgggg
     9181 ttgtactaaa aatactgcaa aaactgtgga tttaattcaa agtcacaaac ctgaacttgt
     9241 tttcagtctt ggggatacct cttatggaaa agatataaac tgttgggtat ctatagttaa
     9301 gccaatatcg gatagaatga aagcggtaat tggcaatcat gatgttatgt caccaactct
     9361 tttagaccaa catctcaaac aatttggatt agataaatca tattattcat ttgactataa
     9421 taacatccat attttgatga tggatactga atcttcctat cttcctggtt cagatcctga
     9481 cttttcagac ctagagaata cagaccacta tcgatttgtt gaaaatgatt tatctattgc
     9541 atccaaaaat ccggcaataa agtggattat tgtaatgagt catagacagt tatattcttc
     9601 tctctgtggg gaccatgact cttgtgaacc aataaaaaaa ttgagagatg cttaccatcc
     9661 cctttttgaa aaatatggtg tagatttact atttagtgga catgctcaca attatcagag
     9721 aacatatcct ttattttata atgatataaa ttcctcacaa cctcttattg aagaagagga
     9781 taaaatagaa tatacgtctt cgaaaggaat ggttcagata atagttggta ctggaggtat
     9841 agattttgat aatttttcta accaagagcc ttttgtagta tatcaggagg attctcaata
     9901 tggattttta aacgtcgatg taattgacca agggaatgta cttttaggaa gatattatgc
     9961 taatacggga gaggtattag atgaatttaa aattgttaaa taaaaaaaat attaattact
    10021 taaatttaac aattatttta ttatagcctt aaatcaaaga aataagcaac tataattcta
    10081 atacaaactc catttggata aattttatat tcaataaaaa agatgtattg aatttctaaa
    10141 ggattctacc gaaccctaaa attagtttct tctataagta acaaattatt atatctactt
    10201 tcacaattta acgagaaaaa aagtatctgc aatatgcttt agtataattt tgaataaaaa
    10261 attagcaaat gattatttta ataacaattc cctaatttag taatcatgtg ttagcaagta
    10321 ttcaaagact tcaattattt ttaacagtct aaaaaatgat atccaaaagt cagtaattac
    10381 cctataatca attttagatg gcctcaataa aataatatgt taactaatct tttaattagt
    10441 tatttatacc tatatcttta acctttgcat caatagtata cgttacagta aatggttcgc
    10501 catttgtttt atgaaaaact aatgcatttc caacaaaatt aaatgaacta gaacttattc
    10561 cagtaccgtt tatttctata ggtacatcaa ctatatctct cagatgagta atggcaatac
    10621 tcttattccc tggtaataag gcatgatagg ggtggccatg taatgagtct agattctgtt
    10681 ctggggcata tgtatgaaga atttctacct ctataggttt acttgcagta aatgttacag
    10741 ttccagacca aacctttcca tcatctctta agggaagagc aaatactacc tcatgagatg
    10801 gttggccttg atgctttaca ggagcgggtt gagacataac cgtcttttgt tctacaaaag
    10861 tgtaggtata attattaaga atagacgata aagaggagtt attgttgttg tttatatctg
    10921 cagatacaag aacaattggg caacaatcac taaatattat ggagatccca gattcagcca
    10981 taaaaaatat aattatagaa actaagaatg atgcttttaa attgacgata tgaagtttat
    11041 atttatcaaa taaattaaac atatttagct ctaaggaatt taattaagtg tctttattaa
    11101 gcgttttgaa agatatattt atgaacgata aaatctaact cttttaatga attagaataa
    11161 gaaataggtt tttatattaa gttagccttt tatttaaaat ttatgttatt catcaatagt
    11221 tcgaattcag gtaaatattt gtcatattga ggagacataa agtaagctat aaacattgaa
    11281 tcagaatcaa ttaatccata tactactaag gtcatcactg agttatttcc taacaatact
    11341 ttgtaaagat ccgtggtcat ctctgtacca tcaatgacat agccaatatc aaaagatcgt
    11401 atttttacaa tatctttatc atcgcctgca gactgtttga acattgattc taccatagaa
    11461 gggagtaact cttctacacc tggcaaaagc atagaatttg aagaaacaat caaaatctga
    11521 gcttgatcgc catttttagg gtagagatcc agatgtaaaa aatctaattg ctcattttca
    11581 tgtgtgatac cccaacctgt aggtaattta aaagaaagaa gcttggaatt ataaaaagta
    11641 ttctcaaggg tgatatcata taatttaaaa ttcagtttgt tttccaggtt ttgattttga
    11701 gttatatcat aatctggtaa tgcatagata aatttgtctg gtgagaatat taataatgtg
    11761 aaaatcacgt taataaatat aattaatatt atttggtggt gcagctccat gattaaatat
    11821 cagtttaata catttaatta aatatttcgt aaattgtttt ccatattaca tagattataa
    11881 taagataaca aaaaactttt acacctttgc caaatttaag tttttgataa aggttgagga
    11941 aactttgtag gaattgtact acctctggga catttagatt ttatacaaga atgaaaagta
    12001 aaccctctta aggtttcctc aaaagacaag tcagaagata attgagttta ttaatggatg
    12061 aatagaaatt aagatctttt aatgatcatg cttgaccttt aaattcagaa tgaatattga
    12121 ctagagttat ttgtgttttt tcggtatgtt tttatctacg attcagttga agttgaacct
    12181 gagttttgtc ttgtttatct tttttttgta ggtttttgtg aacttctgca atcttgtcct
    12241 ccatatagaa tcagcactta tctatctgac ttttttcccc atgtttatct accttttgca
    12301 gttcaaaata ttttttcatc gctttcaaat tatcacaggt agttgataat aggatgattg
    12361 ctaatattac aaatgtcctt gcccttgctt actaaaaagt ttagaatgat tatcctactg
    12421 gtaagtacaa gctgctatta ttttactaga gacaaagaaa tggcatatta aaagagccat
    12481 tgcaatattt ttactttctt ttaagttaga aaattcaaca aatttattta tagagaatta
    12541 taaattatca taaataatag aagaaaattt cttttaaaaa catagtattt aatgaatatt
//
LOCUS       DASVEV010000046         8544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1177119, whole genome shotgun sequence.
ACCESSION   DASVEV010000046 DASVEV010000000
VERSION     DASVEV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177119"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF222_02490"
     CDS             complement(<1..921)
                     /locus_tag="VF222_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141326.1"
                     /translation="MHFWKSSSPQRPMILSRKITVVTGAIMIFIVIIDLLMTRQILPY
                     TNDMEIIMFVLTIAIGYGIGSWILLKYTTQISKKIRAKSRFINIMHWTVIVIQFTLLG
                     ILLLILFSNTSHLFLSPTVFAISSIASSIVIGLIAFKFFSWFKLSNKKNLLILVYGIA
                     ALTLGSSILEDAGTKLLLVEVVQEKSPLGATVESSFLYKPSEKYDGEIIYKIVNSETT
                     TLYIIPNSSLALYNLLNSTVLPIAFGFRWVASTTLLRNLYQRVGKLPLLLWIIFSLPL
                     IFYLIGKAPGFFSGEGLSQVDEQYRYFFRLL"
     gene            1121..1318
                     /locus_tag="VF222_02495"
     CDS             1121..1318
                     /locus_tag="VF222_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141327.1"
                     /translation="MSSNTNRSFSCVFCGVEYNPIKPDDIHIKANKYKINRDDIETTH
                     KCNECGKNTKLYWSEEKGSNE"
     gene            1674..2753
                     /locus_tag="VF222_02500"
     CDS             1674..2753
                     /locus_tag="VF222_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141328.1"
                     /translation="MKYCNYFLVHILILPILFSSISFTSNLPGEVDNNSNNGYNLFSL
                     NFLQNSYAIETDEKDNVKKQDEKNNKDSKETKEDESANNKEGKSVFNFVAVGDWDCTS
                     ETEDTVDNIIKQDPEIVLALGDFSYSGDPDCWFDLIEPIADKTKIVMGNHEDEDDYMD
                     YFGLENQYYSFNQKNIHFLALSTETDFDEESEQYKFAIQDLEKYSKDPFIDWIIVFYH
                     QFIYGSGSGLPEETDFRETYHPLFDKYKVDLALQGHLHVYERTYPITFNQDDEDEPIV
                     QDTNPNLYKNPMGTIFLTVGTGGAHDMQLSSLNDYSAQGIAGEFGILNIEIENDQTPL
                     SGQFIENGKKQKISDEFKITKDNKS"
     gene            3063..4034
                     /locus_tag="VF222_02505"
     CDS             3063..4034
                     /locus_tag="VF222_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141329.1"
                     /translation="MLRNLGILFLGILVLVTLNVTFSFLGTVMAQESEILSKITNDNK
                     QAKHDDDKLVVVANINLKNTEKNGLIKVVGVINGEDFVKNIPLDKIKESTKQLKVKFS
                     MDKENEIVNAGKPDEFFVCAYHVKNADTKVTSHKQGVGEYFDCNEGDIQSTTSPTQIS
                     LFKATSQVYKKTSTYYNLYSKPQVTSFATLANDTTKASNDVSNLKKDTANSGNKDSSN
                     KPVKVKILVPMEDKKDAKKIKVMAMLKGQIKSAVIDNVQEEFSKISGYTIERTFAFDR
                     DTDMGPIQIGDRFHACVIGQDLNPPEGSECEKRLIKHLDKPNTLAAR"
     gene            complement(4263..4427)
                     /locus_tag="VF222_02510"
     CDS             complement(4263..4427)
                     /locus_tag="VF222_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141330.1"
                     /translation="MENDKDIKIETDEEVGLDDKIKAGARALGKKIEDPDKDTGAEYE
                     TEKTKERVVD"
     gene            complement(4932..5231)
                     /locus_tag="VF222_02515"
     CDS             complement(4932..5231)
                     /locus_tag="VF222_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141331.1"
                     /translation="MKVRVYFSKEPESELFIRTNKISNQALITLYDLVWESEFKNILN
                     VGKIWLKFKEERKPFGIPLREKRDHIQIAPGDIIQIAENYYVVDIVGVKKITITN"
     gene            complement(5496..6170)
                     /locus_tag="VF222_02520"
     CDS             complement(5496..6170)
                     /locus_tag="VF222_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141332.1"
                     /translation="MKINNVYYSIILLFITIFPIIAFAEEKSSISNYTSPSSSSSSSS
                     NNTNVDFATNIEFIKSNLSAAISNKQAGNNEITQTHTFPPRSEIYTLIRDQLSSADNE
                     LNKTLIDSLKNLTSIVIKSTPAELADSAASVESLLNKAKIIVIPENEIGDIKFNMSTL
                     INLLKTAEFEYYIGIANGTITNISEYQNPHGFVSSANELLNKTTNSLNHTLVSNLSKI
                     LLQELH"
     gene            6683..7924
                     /locus_tag="VF222_02525"
     CDS             6683..7924
                     /locus_tag="VF222_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7141333.1"
                     /translation="MVFFEKANIKSSFLNKFKTMYRHTHVKDIENVLILQGGGSLGAF
                     ACGACKTFAKKDIKFDIIAGTSIGAINGAITAGSKNDNPAKDLEDFWIEIAESSYNII
                     PDIFSFDYDYQNNQINFKRSSSASLNAAFFGVPKFFVPRWFNWKKYNYNSSNNFEYSM
                     LPYNWTYIYDNTLLEKTLEKYIDFKKLSPDAKPKKINDNDGSDNTIRLIITAVDVLSA
                     EPVIFDSYKTQIQMKHLLATIGYPQYGFPWIEVNDGVYTWDGSLLSNTPIREVMMASP
                     SNDKNIFVIENYPKKIDKLPSNMNEVQSRAKDIMFTDKDQSLGKMSRLITRHINLIET
                     LYDIFEEHDHSKIDKDIIKYVEKEHKLLVEKYGAKILKIKRISRENIETPYSLQNADF
                     SVNTIKDLIIQGENKALTSLN"
     gene            8245..>8544
                     /locus_tag="VF222_02530"
     CDS             8245..>8544
                     /locus_tag="VF222_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7141334.1"
                     /translation="MKFTKIVETCIYSSKLKEMKNFYINKLGLDFVSEENGRHVFIKA
                     GKSMLLIFNPESTLNDSNSMFPVHGAITPPSIVHFALEINIADYEKWKDLLQKNQI"
BASE COUNT         3283 a         1081 c         1244 g         2936 t
ORIGIN      
        1 taaaagtcta aagaaatagc gatattgttc atctacttgt gataaacctt caccagaaaa
       61 aaaacccgga gcctttccaa tcaaataaaa tatcaatgga agagaaaaaa tgatccaaag
      121 taacaatggg agtttgccta ctctctgata aagattacgt aaaagtgtag tagacgcaac
      181 ccatctaaat ccaaatgcta taggaaggac agtagaattt agtagattat ataatgcaag
      241 actcgaattt ggtattatat agagagtagt agtctctgaa tttactatct tgtatattat
      301 ttctccatca tatttctcag atggtttata taaaaatgat gattcaactg ttgctcctag
      361 aggtgatttt tcttgtacta cctctaccaa tagcaattta gtcccagcat cttctaatat
      421 tgatgaacca agtgttaatg ctgcaatacc ataaactaaa attaacagat tcttcttatt
      481 acttaattta aaccaggaaa agaatttgaa tgctatgaga cctatgacaa tgctcgatgc
      541 tatagaacta atagcaaaaa ctgtgggaga caaaaaaaga tggctagtgt tactaaaaag
      601 aattaacaaa agtattccta ataaagtaaa ttgaattaca attactgtcc agtgcatgat
      661 attaataaaa cgagatttgg ctctaatctt cttgcttatt tgtgtagtgt attttaaaag
      721 tatccaggat cctataccgt atccaatagc aatagtcaaa acgaacatga tgatttccat
      781 atcgttcgta tagggtaaaa tctgacgagt catcaacaag tcaataatta caatgaaaat
      841 cataatagct cctgttacaa cagttatttt tctactaagt atcataggtc tttgagggga
      901 gctgcttttc caaaaatgca cgtattagat agattttaga attatataag agtaatgagg
      961 acaaacaatt cgttttaaaa atttgattct attaacaata ttataaaatc taaagtataa
     1021 taacgataat ttttttttga aaccccttcc tacattatat aaattattaa aaaaaactac
     1081 aatgataaaa tttagattaa gtatgtcatc tataaatatt atgtcatcta atacaaacag
     1141 atcttttagt tgtgttttct gtggagttga gtataatcct ataaaacctg atgatataca
     1201 tataaaagca aataaatata aaattaatag agatgatatt gaaacaactc ataaatgtaa
     1261 tgagtgtggg aagaatacta aattatattg gtctgaggaa aaaggatcaa atgaataggt
     1321 tgtctaaaaa gctaatatgg tgaaattgat aaaatcaaga ttttggacag attacattat
     1381 ataaatattt tatactaaga gggtaagacg taaaaaagtt atgttgttaa cttgtgatta
     1441 aaaatttatt cataaatgta atagtagaaa taataaattt gaaatatcga gaatttatta
     1501 gtaaatgtca aataataaga tcatttttaa tctatcttag tagttctttt atttaaacaa
     1561 ctacagatag ttaaaagaat caactaaatc ttatggatta tttttccatt cattttatca
     1621 ataaaaagca gtacaaatta ttaaaagtaa tagacgatca attatgtgtg taattgaaat
     1681 attgtaatta ttttttagta catattctta tacttcctat attattttct agtatctcat
     1741 ttactagtaa tttacctgga gaagtagata ataatagtaa taatggatac aatcttttta
     1801 gcctaaattt tttacaaaat tcttatgcaa ttgaaactga tgagaaagat aatgtaaaaa
     1861 aacaagacga aaaaaacaat aaagattcta aagaaacaaa agaagatgaa tctgctaata
     1921 ataaggaggg aaaatcagtt tttaattttg tagccgttgg agattgggat tgtacttctg
     1981 agacagaaga tacagtagat aatattatta aacaggatcc agaaattgtt ttagctcttg
     2041 gtgatttctc atatagtgga gatccagatt gctggtttga cttaatagaa ccgatagcag
     2101 ataaaaccaa aattgtgatg ggaaaccatg aagatgaaga tgattatatg gattattttg
     2161 gtttagaaaa ccaatactat tcctttaatc aaaaaaatat tcacttttta gcattatcaa
     2221 cggaaaccga ttttgatgaa gagtctgaac aatataaatt tgcaatacaa gatcttgaaa
     2281 aatattctaa agatccattt atagactgga taatagtatt ttaccatcaa tttatctatg
     2341 gatctggaag tggtcttcca gaggaaacag attttagaga aacatatcat cctctttttg
     2401 ataaatataa agtagatttg gctttacaag gtcatcttca tgtctacgaa agaacgtatc
     2461 caattacttt taaccaggat gatgaagacg aaccaatagt acaagatacg aatccgaatc
     2521 tatataaaaa tccaatggga actatctttt taactgtagg aactggagga gcacatgata
     2581 tgcaactgtc ttcgttaaat gattattcag cgcaaggaat agctggtgaa tttggaatac
     2641 taaatattga aatagaaaat gatcaaaccc ctctttctgg tcaatttatt gaaaatggaa
     2701 agaaacaaaa aatttcagat gaatttaaaa taacaaaaga taataaaagc taagtcaaat
     2761 tagttatatg ctatatttga agttacataa tatgttgaaa tttcctattg atagtgatag
     2821 tatattggaa gtattggtaa ctcctatata ttaatataaa aatctaccga accacgtata
     2881 tcaatcactt gtataatgta ggatatgagc ttgaataaaa ttgacctaaa aaggacaaaa
     2941 aaactctaaa ccaaataaaa atagatctat attatataaa aataatcaac tactatatta
     3001 aatctttatt ttaaataaaa tattttcaaa attaataaat tgaaaaattt caatgagagt
     3061 atatgctaag aaatttagga attttatttc taggaatttt agttcttgtc accctaaacg
     3121 tcaccttttc ttttctagga acggttatgg cacaagaatc tgaaattctc tcaaaaatta
     3181 caaatgataa taaacaagct aagcacgatg atgataagtt agttgttgta gccaacataa
     3241 atttaaaaaa tactgaaaaa aatggattga ttaaagtagt tggtgttatt aatggtgagg
     3301 attttgtcaa aaatattcct ttagataaaa taaaggaatc aaccaagcaa ttaaaagtta
     3361 aattctcaat ggataaagaa aatgaaatag taaatgctgg caaaccagat gaattttttg
     3421 tgtgtgctta tcatgttaaa aatgctgata caaaagttac ttctcacaaa caaggggttg
     3481 gcgaatattt tgactgtaat gaaggtgata tacaatctac tactagtccc acacagataa
     3541 gcttatttaa agcaacttct caagtttata aaaaaacttc tacatattat aatttatatt
     3601 caaaaccaca agtaacatcc tttgctacac ttgctaatga tacaaccaag gctagtaatg
     3661 atgtatctaa tcttaaaaag gatactgcaa atagtggtaa taaagatagc agtaacaaac
     3721 cggttaaggt aaaaatatta gttccaatgg aagacaaaaa ggatgctaaa aaaataaaag
     3781 ttatggctat gcttaaaggc caaattaaat cagcagttat tgataatgtt caagaagaat
     3841 ttagcaaaat tagtggatat acaattgaaa gaacatttgc atttgacaga gataccgata
     3901 tgggtccaat acaaatagga gatagatttc atgcgtgtgt aataggacaa gacttaaacc
     3961 ctccagaagg aagtgaatgt gaaaaacgac taataaaaca ccttgacaaa cccaatacct
     4021 tggcggctcg atagtaacta taatttgcca taattatttt ttttgaacct ttaaagatcc
     4081 aacatataag aatgagattc gaattacaaa atataatacc ctcaacctat gaatcaacaa
     4141 ttggaattat gaatttacat tgaaattagt agagtattaa catttattgc aataaaaaaa
     4201 aatttacatt actatattac tatcgattaa aaataatatg ttgaatggat tgaatagtat
     4261 attcaatcaa caactctttc tttagttttt tctgtttcgt attctgcacc agtatcttta
     4321 tcaggatctt caatcttttt acctaatgct cttgctcctg cctttatttt atcatccaat
     4381 cctacttctt catctgtttc aatttttata tccttatcgt tttccattgt aattgttgtt
     4441 gtatggtact atatataagt tagataataa atattctgat aaattttata tattatttgt
     4501 ccattatttt tagactatat aataaatata attactttat tctttgttct tgtagtctaa
     4561 tctatttaaa aaccatcgct actaaggacc atcaataggg aaggctatgg ggagattgaa
     4621 aaatatctta ttactttatg aattatgaca aatatatgtt atagcctaat caaaatgtaa
     4681 ccaattctta ttaacatatg acaagtctat cactacagta caacgacttt taaatattca
     4741 caagttataa aaaaattcta catatatata caaatattat taagaaattt aaggggcaga
     4801 ctatgtcaat aggccaatga taaataaatt attatctttt agattgctga gatgttataa
     4861 tagtcgactt gaacaataat cattattgaa gaccaaacaa tatattttta ttatatagac
     4921 aactaaataa gttagttagt tattgttatt ttttttactc caacaatatc aactacatag
     4981 taattctcag ctatctgaat aatgtctcca ggggctattt ggatatgatc tctcttttcc
     5041 ctaagaggta ttccaaaagg ttttctttct tctttaaatt taagccaaat tttacctaca
     5101 tttaagatat tcttaaattc tgattcccag actagatcat agagagtaat aagtgcttgg
     5161 ttactaattt tgttagttct aataaacaac tcagactcag gttctttaga gaaatatact
     5221 cgaactttca tgattaagtt cctaaaatat ttattatatt tattaattat atataattaa
     5281 ctattaaata gttaaatgta ttagtccacg acactaagct ccaaattata atcatatttt
     5341 tcaaatttga aaatgttagg ttcatatgat attatacata ttatacaggc atgtaatgtc
     5401 aatgtatact cttttaaatt gtaataagtt acatttgacg ataaagtaac atacatattt
     5461 tccataaaaa gagttaaaga ttgataaata agtctttaat gtagttcttg aagtaaaatt
     5521 ttactcaaat tactaaccag tgtatgattt aaactatttg ttgttttatt caataactca
     5581 ttagcagatg aaacaaatcc gtgaggattt tggtattctg aaatgtttgt aattgtacca
     5641 tttgctattc ctatataata ttcaaattca gcagttttaa gtaaattaat caaagtgctc
     5701 atattaaatt taatatcacc aatttcattt tctggtataa ctataatttt tgctttattt
     5761 agtaaactct ctacagaagc tgcagaatca gcaagttcag ccggtgtcga tttaataact
     5821 atggatgtta gattttttag tgaatcaatt aaagttttat ttaattcatt atctgcagat
     5881 gataattgat ctctaattaa tgtatatatt tcagatctcg gaggaaatgt atgtgtctgt
     5941 gtaatttcat tatttcctgc ttgtttattt gaaattgcag cagataaatt acttttaata
     6001 aattctatat tagttgcaaa atcaacgttg gtattatttg atgatgatga tgatgatgat
     6061 gatggggaag tgtaattact aatactagat ttttcttctg cgaatgctat tataggaaat
     6121 atagtaataa ataatagtat aatagagtaa taaacattat ttattttcat atttagccta
     6181 aaatatgtaa aaatgtaaaa tatataaatt attccttatt tctttgacca aatttactca
     6241 tggcttatca tattttatta aggtgatgtg acaagtgata ggttagtcat ttattatgtt
     6301 ctacattgga tacaacttca ttgaagataa ggaggtgaat attattaaat atagtactgt
     6361 ttaagactaa tagctcaaat tggtaattag ccagtggggt aggagactaa atttattcta
     6421 atttttattt tattttattt caacaagatt ttctttatcg ttaataagaa ttgatctctg
     6481 aataataata ataatgatac ttttaaaaca atgagaatat tgtaaattac taatttttaa
     6541 aattgattgt ttacatgttt ataatattga gtatattttg gatttacagt agaggataat
     6601 cacacctaaa aagtgttggt attaattctt aatttcagct atctgcgaaa tctctatctc
     6661 aaatataaaa gaaaagacat ttttggtatt tttcgaaaaa gcaaatatta agagttcttt
     6721 tcttaataaa ttcaaaacaa tgtatagaca cacgcatgta aaagatattg aaaatgtatt
     6781 aatattacaa ggtggggggt cacttggtgc tttcgcatgt ggagcttgta aaacctttgc
     6841 aaaaaaagat ataaaatttg acataatagc tggaacatca ataggagcaa ttaacggagc
     6901 gataactgct ggaagtaaaa atgataatcc tgcaaaagat ttagaagatt tttggataga
     6961 gattgcagaa agtagttata atatcatacc agatattttt tcttttgatt atgattatca
     7021 aaacaatcag attaatttca agagatcctc ttcagcatca ttaaatgcag cattttttgg
     7081 cgttccaaaa ttctttgttc ctcgatggtt taattggaaa aagtataatt ataattcttc
     7141 gaataatttt gagtatagta tgttgccata taattggact tatatatatg ataatacttt
     7201 actagaaaaa acattggaaa aatacataga ttttaaaaaa ctgtctccgg atgcaaagcc
     7261 aaaaaaaatt aatgataatg atggttccga taatacaata cgacttatca ttaccgctgt
     7321 tgatgtactt tcagctgaac cagtaatatt tgacagttac aaaacacaga tacagatgaa
     7381 acatctctta gctacaattg gatatcctca atatggattc ccttggatag aggtaaatga
     7441 cggagtatat acgtgggatg gatctttgtt aagtaatact ccaatacgag aagttatgat
     7501 ggcttctcct agtaacgata aaaatatatt tgtcatcgaa aattatccta aaaaaatcga
     7561 taagcttcca tcaaatatga atgaagttca atctagagcc aaagatataa tgttcactga
     7621 taaggatcag agtctaggca aaatgtctag actcataacc agacatataa atttaataga
     7681 aacattatat gatatttttg aggaacacga tcattctaaa atagataaag atataattaa
     7741 atatgttgaa aaggaacata aattattggt agaaaaatat ggtgcaaaaa tcctaaagat
     7801 caaaagaatt agtagagaaa atattgaaac cccatattca ttacaaaacg ctgacttttc
     7861 cgttaataca attaaagatc ttattattca gggtgagaat aaagctctaa ctagtttaaa
     7921 ttaattatgt ttattaaact cagtacttga taatactgct agaagtgcaa attatatttc
     7981 tgataaatgt aaaacatatt tgaaagaaat aagaaagcaa taattcagta ttgtatcctt
     8041 gatgattata gagttttttt gtaagtgtgc gattgatatt taaaatatac aatcaataac
     8101 caattcatat tagatatcaa tctatttgat taattagttg gatgtatatt cttgagtaag
     8161 ataggttttg aaacacgttt gcattctttt atagttaaga aacgtagata cattaattta
     8221 ttcaaaatga aaaataacaa atgaatgaaa tttacaaaga tagttgaaac atgtatctat
     8281 tcgtctaaat taaaagaaat gaaaaatttt tacattaata agttgggctt ggactttgta
     8341 tccgaagaaa atggaagaca tgtgtttata aaggcaggaa aaagtatgct tttaattttt
     8401 aatcctgaaa gtacactaaa cgattctaat agtatgtttc ctgtacacgg tgctattacc
     8461 ccaccctcaa ttgttcattt tgctttagaa attaatatag cagattatga aaaatggaaa
     8521 gatcttttac aaaagaacca aatt
//
LOCUS       DASVEV010000047        14228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1371870, whole genome shotgun sequence.
ACCESSION   DASVEV010000047 DASVEV010000000
VERSION     DASVEV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371870"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(613..1038)
                     /locus_tag="VF222_02535"
     CDS             complement(613..1038)
                     /locus_tag="VF222_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141335.1"
                     /translation="MTFDKCWDNKMLKRRILIIDDDPDINNLFKLFLEHDGYKVNAYT
                     DPLDALYQFRKNTFDLVLLDLKMPKMSGMLLYQKLRNIDPNLLFCFITADKEYIQHLK
                     KSITEIEKIVIYKPILLSDLRRKVNSLLSEKKSKLLIMT"
     gene            1400..2095
                     /locus_tag="VF222_02540"
     CDS             1400..2095
                     /locus_tag="VF222_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7141336.1"
                     /translation="MTTKFHDRKEAGLLLAQKLKLYIKDAFGSEAKKESFIVMAIPRG
                     GVILGDIIASELNVELDIIICKKIGAENNPELAIGAVMADGSYIINEDIVNRINTSVG
                     YISKQVEIQKKEIERRLMEFRGNQEYHNKLKDKIIILVDDGIATGATILAAVKWLKEK
                     HYCKRLVVAVPVAPLESSIVNKLNQIADKVIVIFSPYDFAAVGQFYEHFDQVSDKEVK
                     EIMNKYGFNSKVF"
     gene            2672..4147
                     /locus_tag="VF222_02545"
     CDS             2672..4147
                     /locus_tag="VF222_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141337.1"
                     /translation="MSKRDYQERSSLDFSFTNPLAESTYEWYQFHKKLHLFRWWIKYK
                     LGQTDEAVFKNRIIQVGNSLGISTKWLNKTLEHAITEFSSNGLGVDYYGYHNIKHELE
                     ATYFTLHAVFGQKNNNRNNNFDSMDIKYLFTSALYHDFDPLKRFEKPNEDAVESYIRS
                     DKKIKESIDEAGLDIDIVIAIIHRTAYPFQGEIAEHARERMNQLFTDAGIAAHDTEKR
                     KHYEDLGWFLSVSERVAGYALDGFEHALELARRNAHGLGWHPSRINKESVKFFSLLNK
                     EKEMLDFVLGGISEKYKENYQNNIDKFKESFLKEIEIRNAVRKRQINLVFNPEENKNI
                     NPITLNEVLSLYKELNNLIEISNEKKFARSINHSKTILVSLRLNEINGRVIGFAKGGP
                     LEYYKLRRGTVEENFGQKNTVFLERINIKTGYWGESGGHNLRMKFLAESKRNGYKFVT
                     GYVHRDVLIRRINNGEPMRIIQKYDPDKLDYYRLYLDNFKI"
     gene            complement(4288..4806)
                     /locus_tag="VF222_02550"
     CDS             complement(4288..4806)
                     /locus_tag="VF222_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7141338.1"
                     /translation="MTNITFQPGTILIISASFLFYMMYLISPTLAYSTDSFEEANAMQ
                     RAYDFLNHGEIEYALQLYDDILKVNPTHIKALNYKGLALASLGHYKEAIPWYDKVLDI
                     DPNDISALNNKGVALYNLGYYDEAIQWYDKVLDIDPNDSDAKYNKSIALQSLNKSNES
                     TVLYNESFEATK"
     gene            5867..5989
                     /locus_tag="VF222_02555"
     CDS             5867..5989
                     /locus_tag="VF222_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141339.1"
                     /translation="MKKIIDHIQCDEKDMEMNKVIKIQDRNAQNIFSAKKNSGS"
     gene            6227..6643
                     /locus_tag="VF222_02560"
     CDS             6227..6643
                     /locus_tag="VF222_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141340.1"
                     /translation="MNKKKHQGKSNSSSNTSFPENNNLFRNTRRISNDIIDIDDSPDV
                     ATWNKEVTRNKDHITKATSRSEETNEKYRNTKSTPEIPPTTTIGLRKENNDDKDDQVD
                     PANQKDLDKIYNSGGKLTDQANLFNNKRKFNKSQKK"
     gene            6805..7092
                     /locus_tag="VF222_02565"
     CDS             6805..7092
                     /locus_tag="VF222_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141341.1"
                     /translation="MHESREKLIKFHGTEVKNIIINFLRSQINNPSTKVIEEFNAIKS
                     PENCETINVQVVADYVTITFYKNESSNILIRRELIPTHNIEHILIDDLRER"
     gene            8433..9041
                     /locus_tag="VF222_02570"
     CDS             8433..9041
                     /locus_tag="VF222_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7141342.1"
                     /translation="MQKQNNITKIRKVENNINPLFVNRWSPRAMSGEEISHDDLMGLF
                     EAARWAPSSYNNQPWRFIYAKRNTDHWQRLFSLLVEGNKIWAKNAAVIVVVISRKNFE
                     FNEKPAITHQFDAGAAWENLALEATLRELVTHGMQGFDFNEARKALGIPDSFDVMAMF
                     AIGKLGQKENLPRDLQEREYPSNRKPLEEIVMEGKFIGRSIQ"
     gene            complement(9550..9813)
                     /locus_tag="VF222_02575"
     CDS             complement(9550..9813)
                     /locus_tag="VF222_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141343.1"
                     /translation="MKETGLTIILMVVINTSLITIIPVTAQLDQPPELTKAGNVTSTI
                     KPQGTSLGEENKSSMSEDSSQTTNAGNVTSTIKPQGTSLDNPN"
     gene            complement(10410..11234)
                     /locus_tag="VF222_02580"
     CDS             complement(10410..11234)
                     /locus_tag="VF222_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141344.1"
                     /translation="MSSKKQIEHTSPLRYLILSMFISIMIVNLITLLLDVKYRHSFVL
                     LILNIAAGTASSLGIIAIIRYGFSGIHGKSYLFLTIGIILWFTADIIVTYYFIIDVKE
                     LIDVSIVDAFYFMGYGFLSLHLFVIMKELQREINPIHAIIISIVTIIFVIYVVTNLLF
                     SEFDLNDNFISIVVSLMYPILDLILIAPSAIILISLRKNYQQSIPWFLSSLSLFINAI
                     ADDGYAIDFVNGNSENLWVWELFYVADFIIMSGALYWYNKFHIFHHNKKDTSLRNK"
     gene            11581..12720
                     /locus_tag="VF222_02585"
     CDS             11581..12720
                     /locus_tag="VF222_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX7141345.1"
                     /translation="MRLIVYTGKGGTGKTVTSCATAIGLAEIGYKTLLISADPAHTVS
                     DAFCIDIGHDTKTISSNLDALEIDPMVEISKDYVDIISSISSSFIGKGVNSSIAYEIA
                     MLPGMTQLFSLIKIEEVIRENNYEKIVLDMPASGEALKFLYFPKLMGSMARHMTGLTN
                     LFSGFAKMFQPLSSYSTYLDSFITQTDILKRLDNLFSILSNQTITSLRLVTNLNNFSV
                     KNAKRALMSANLFGINVDLVIINKVEVIDEDKRIFLEKIKLNFFPLPCREAIRMKEEL
                     QGIEMLKQHNYHIFGTDDPSNIFFTEKVYSIEENNEYITLKFKIPFANNASLDIQKKS
                     DELMITLEHDRGILTNTITLPFAAVTMKIVSSEVVNDNLVVILKR"
     gene            13086..13868
                     /locus_tag="VF222_02590"
     CDS             13086..13868
                     /locus_tag="VF222_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7141346.1"
                     /translation="MKVSFNPKTYLGRMTIIFISIFAILIIVSTIHGTILINAQLDST
                     YALNDTLFVSGSATANTKADKVVVSLGVETTDKMAEKALVSNSILLNRVLAALNESGV
                     QQNETSTYSFSIKPNYNYSNYGDRGKLTGFTVSHSIHIESSNIDSVSQWIDTAVQAGA
                     NTVDDIYFSVSEDKLQKIKNTLLKEAVSNAKSKADIVAAAAGLNVAGIKSITVGGEMG
                     LPPVPGPLYTKSVSSDEASSTPILAAEQEVSTTVSIVYIVGR"
BASE COUNT         5244 a         1828 c         2069 g         5087 t
ORIGIN      
        1 gttcattttt gtggcttctt gattcattga tatcttttgt ttgggagtta acttcagatg
       61 cctgttctgt tttactagat gttacactag aagtatctat ttccttataa tcatcagaaa
      121 ttgttgattt ctcagacata ttaaatacta atacatttga agtataaaag gtgattctat
      181 aataaatgat tcgataataa gtgatattta atttttattt tgatattatt attagtatta
      241 ttaattgttt tactactcct gtgtcttttt tactactgta ttgaattaaa tttcctttgt
      301 aagggaagtg ttgtcttttt tcaatgaatg ctgttgaaat taatagccat cttttaaaac
      361 attaaattgt tttaaaatta ccgctcaact aagataaagc aattttattt catttattat
      421 taacatcata ataagttaaa gaaaaaattc ttgttcattt ttcctcttct gttagaattt
      481 aacctcaaaa ctttcaatcg aaattgtttt tggataattt ttatatcata gattatacaa
      541 tttcacagtc gattataaat caagatctat atagaattaa aagcataaaa taaatcgata
      601 aaagtaactt attcaagtca ttatcagtaa tttagacttt ttttctgata aaagtgaatt
      661 aactttacgt cttagatcgc tcaagagaat tggtttatag atgacaatct tttcaatctc
      721 agtaatactt tttttaaggt gttggatgta ttctttatct gcagtaataa agcaaaataa
      781 aagattagga tctatatttc gtaatttttg atataatagc atacctgaca tcttcggcat
      841 tttcaaatca agtagaacta aatcgaaagt attttttcta aattgataca gtgcatctag
      901 tggatcggta taagcattaa ccttatatcc atcgtgttct aagaataatt tgaataaatt
      961 attaatatca ggatcgtcgt ctattattag tattcttctt tttaacattt tattatccca
     1021 gcacttatca aaggtcaata tcctatatct atattaagga aatttttctt gtgtatatat
     1081 gagatatcat aattaataga taaaaatttt atattgaaag gactataaaa tatagaagta
     1141 tgcacttatt gattacacaa catattttga acctattatt attgatatat taatttatat
     1201 ttcagacatt atcctataga gagttatttt ttgattggtc acattattag ttatacagtc
     1261 tgataacttt agttttttaa tataaaatta ttataaacaa aaggtttaat ttattaaaat
     1321 aaaaagtgtt taatattata aataactgag acctaaaaaa gtctaaatga gtgtatataa
     1381 ttaattttct cataattata tgacaactaa atttcatgac agaaaagagg caggactatt
     1441 gcttgctcaa aaactaaaat tatatattaa agatgccttc ggctcagaag caaaaaaaga
     1501 atcttttatt gttatggcca ttcctagggg aggagttatt ttaggtgata taatcgcctc
     1561 agaactcaat gtagagttgg atatcattat atgtaaaaaa ataggagcag aaaataaccc
     1621 agaacttgca attggtgctg taatggctga tggaagctat atcatcaatg aagatatcgt
     1681 taacagaatt aatacatcgg ttggatatat atcaaaacaa gtagaaatac aaaaaaaaga
     1741 aattgaacga cgattaatgg aatttagggg taatcaagaa tatcataata aactaaaaga
     1801 caaaataatc attttggtag atgatggcat tgcaactgga gcaacaatcc ttgctgctgt
     1861 caagtggtta aaagaaaaac attattgtaa aaggttggtt gttgctgtcc ctgttgcacc
     1921 gcttgaaagt agtatagtaa acaaactcaa tcaaattgca gataaagtaa ttgttatttt
     1981 cagtccatat gactttgcgg cagtcggtca attttatgaa cactttgatc aagtgagtga
     2041 caaagaggtt aaagaaatta tgaataaata tggttttaat tcaaaggtat tttaattctt
     2101 agattcaaag gttgttagct caatttctta gtcatcaatt taagattaat ataaataaaa
     2161 aacatcatca tagtatttgt tacaattact tttttctatg aaaaaaatga tatataagat
     2221 tttgaaactt acaatccata aaatatgact taagttaaaa tcaattgtgg tagcactgct
     2281 gaatattcac ttataaatac agatcatcta tttgctaaga agaagcgaaa tatagtcgac
     2341 taaattagaa atcatataaa ttatttcata aataatattg tcaattataa tatttgtata
     2401 ttgtgaacca ttgataagat aataaaattc ccttacaata tagaaattag ttccagtcaa
     2461 tctacatgtt ttactgaaga ataatataaa attcacttcc tgactaatcc acctatattt
     2521 gttaagttat atttttatag tttatttgaa ataattacat gatatttgca aactatgcaa
     2581 aagaattcga tatacaaact taatcttata aaataaaata tttataatca ggtttttatt
     2641 atattactta tatttggtgt ttaaggtagt attgtctaaa cgagattatc aggaaagatc
     2701 tagtttagac ttttcattta ccaatccact tgcagaatca acctatgaat ggtatcaatt
     2761 tcataaaaaa ttacatcttt ttcgttggtg gataaaatac aaattgggtc aaacagatga
     2821 ggcagtattc aaaaatagaa taatccaagt aggtaatagt ttgggtattt caacaaaatg
     2881 gttgaataaa acactggaac atgcaataac agaattttcc tctaatggcc taggagttga
     2941 ttactatggt tatcataata taaaacatga attagaagcc acttatttca ccctacatgc
     3001 agtatttggc caaaagaata acaatagaaa taacaacttt gattcaatgg atatcaagta
     3061 tctgttcact tcggctcttt atcacgattt tgatcccctt aaaaggtttg aaaaaccaaa
     3121 tgaagatgct gttgaatcat atataagaag tgacaagaag attaaagaat ctattgatga
     3181 agcaggttta gatatagata tagttattgc aattatccat cgaacagcgt atccctttca
     3241 gggtgagata gctgaacatg caagagagag aatgaatcag ttattcacgg atgccggtat
     3301 agctgctcat gatactgaga aaagaaagca ttatgaagat ttaggttggt ttctttcagt
     3361 ttcggaacga gttgctggtt atgccttaga tggttttgaa catgctttag aactggctag
     3421 acgtaatgct catggactag gatggcatcc atcacgtata aacaaagaat cagtcaagtt
     3481 tttttcttta ctaaataaag agaaagaaat gcttgatttt gtattagggg gaatctctga
     3541 aaaatacaaa gagaattatc aaaacaatat agataaattt aaagaatctt tcctaaaaga
     3601 aatagagata agaaatgctg ttagaaaaag acaaattaat ttagtgttta atccagaaga
     3661 aaataaaaat atcaatccaa tcacgttaaa tgaagtttta agtctttata aggagcttaa
     3721 caatcttatt gaaatcagta acgagaaaaa gtttgccaga tcaataaacc attctaaaac
     3781 catccttgtc agtttaagat tgaatgaaat taatggaaga gtcattggat ttgccaaagg
     3841 tggcccactg gaatactaca agttaagaag aggaactgtt gaagaaaatt ttggacagaa
     3901 aaatacggtt ttcttggaaa ggataaatat taaaacaggc tattggggag aaagtggagg
     3961 tcacaatctt agaatgaagt ttttagctga atctaaaaga aatggatata aatttgttac
     4021 cggttatgtt catcgagatg ttttaatccg tagaataaac aatggcgaac ctatgagaat
     4081 aattcaaaaa tatgatcccg ataagctcga ttactatagg ctatatttag ataatttcaa
     4141 aatataaaat tatgtcatag aattaagatt atttgacttt aaattccatt gacttgaaaa
     4201 taaaatttcc caagtataat aaaattatta taattcctgc gactcattaa aattttagtt
     4261 attatttttt acgtaaatac tttatagtca ttttgttgcc tcaaatgatt cattatacaa
     4321 gacagttgat tcatttgatt tgtttaagct ttgtaaagct atgcttttat tatatttagc
     4381 atcactatca tttgggtcaa tatcaagaac cttatcatac cattggatag cctcgtcata
     4441 atatcctaaa ttatagagtg ctactccttt gttatttagt gcagaaatat cattgggatc
     4501 aatatcaaga accttatcat accatgggat agcttcctta taatgtccta agctggccaa
     4561 tgctaaccct ttgtaattca atgccttgat atgagtagga tttactttaa gaatatcatc
     4621 gtataattgt aatgcatatt caatttctcc atgatttaga aaatcatatg cacgttgcat
     4681 cgcgttggct tcttcaaaag agtcagtgga ataagcaagt gttggagaaa ttaagtacat
     4741 catatagaat aagaaagaag ctgatataat taaaattgtt cccggttgaa atgtaatgtt
     4801 tgtcacgtgg cttataattt accccgaaat atttaagctt tatttcttat aaccattgaa
     4861 tataattatc aaaatctaat aatttgacag ttaaaaagag aaagtacatt ctgaagatac
     4921 tatcagtttc taacaaggaa tcctatccta atgatgagta ttatttaaat ctgatttgca
     4981 tgtttaattt tgttggtaaa acattttttg tgactatgtt ttaatgacta caaaatggta
     5041 tattatacta ggagtaattt tgtttattta taccatctct tagttacaaa ttttttctct
     5101 ttgaattata ttagctgtaa tatcattctg tgtactattt gttaattgca tttgagtaga
     5161 attattttca ttattaacca attattccaa attaggtcat atataaaaaa gataccttcg
     5221 cttataaata aatcatagta tagtaaaacg cgtgtttgga ttattattaa ttacaaaggc
     5281 ttttttactt taatactgct ttcgctggta tagtaataca gtaccaatag gtaagaatac
     5341 aggaatagct aatataattg tgaaaactat tgtaatttca tagctatcat taattttaaa
     5401 ttttaaatat gcttcaacaa gcttattttt atgaaacttt tataatatgt aatagtagat
     5461 attgaatttt aacaataaat cttggaggag attcattgtt aaaaaattca atattaggcc
     5521 tatgaagtag aattaaatat aatactgact tttagaaaat gaaataatct tattttagtg
     5581 tgttatagga aattgatatg tcgaaggaat ataatttaaa tcatttatct ttgataattc
     5641 attaaaaaaa gtattttata tataatcatc cttatttgat gcatgaagta ttcatcctga
     5701 tttttttctc tactaagttc actataagct tctatgagaa ttaataaggg attttatcta
     5761 tggattatat ctatggatta tatctatgga ttatacctat gatcatctac ttagacctcg
     5821 gctaaaaagt ttaaatgaaa aagattctat aatgatttac agataaatga aaaagataat
     5881 tgatcatata caatgtgacg aaaaagatat ggaaatgaat aaggtaataa aaatacagga
     5941 cagaaatgct caaaatattt ttagtgcaaa aaaaaattca ggttcttaat taatcaataa
     6001 aatttttgag gatacccgag cctaaacatt tgcattgatg gttgttgatg gtatgttaaa
     6061 attgtaggtt gattcactac aactctccaa attgtaacca aaaatgacac tgaaacaagt
     6121 aataaatatg ggtttccaga cttgtgagtt ttttattatt aaacatttca ttgatattta
     6181 aaaaaaatat aactaaaata aactattctt tatactaaaa tatagtatga ataaaaaaaa
     6241 acatcaaggt aaaagtaact ctagctcaaa tacttctttt ccagaaaata ataacctgtt
     6301 tcgaaataca aggagaatat cgaatgatat aattgatatt gatgattctc ctgatgtagc
     6361 aacttggaat aaagaggtca cgagaaacaa agatcatatt acaaaagcta ccagtagatc
     6421 agaagagaca aacgaaaaat atagaaatac caaaagtact cccgaaatcc ctcctactac
     6481 tactattggt ttgagaaagg aaaataatga tgataaagat gatcaggttg atcctgccaa
     6541 tcagaaagac ttagacaaga tctacaattc tggaggtaaa ctaacagatc aggctaattt
     6601 atttaacaat aaaagaaaat tcaataaaag ccaaaaaaag taaataagac aagacatttt
     6661 ttgaaaaata tgaatgttta ttgataagaa aaataatctg aaaaatattt attgctagat
     6721 catagatttt aagagatata cctatgttat taactagttt aatatcatcg aaacattttt
     6781 tgcttttgtg atataatcat attcatgcat gagagtagag agaaactaat aaaatttcat
     6841 ggcacagaag tcaaaaacat tattatcaat tttttaagga gccagattaa taatccttcc
     6901 acaaaagtaa ttgaagaatt taatgcgatt aaatctcctg aaaattgtga aactataaat
     6961 gtacaggtag ttgctgatta tgtgacaatc actttttaca aaaatgaaag ttcaaatatt
     7021 ttaataagaa gagaattaat cccaacacat aatatagaac acatcttgat tgatgatttg
     7081 agagaaagat agacctttta ttatatcctt acggtttaaa tattctatac aaaaaccatg
     7141 atttgtataa taaaaatgac ttttatttaa aagagaatct aagattaaaa aaactagtca
     7201 atgtaaataa acccatttat aatatacgaa attttatatg ataaagcata aagttagtaa
     7261 agtttaaatt ttttctaaat tttataaatt ttaaaataat tacaattaga caatctttat
     7321 ttggatttat atcaattaat ataattatag ttaatcttat taataagtta tagcctttat
     7381 attgtaatag catgtgtgac tgctataaaa tacctgaaaa agttagaata aaatataaca
     7441 gacttttgtc aaaaaaaagt gaagagagta ttgaagaaac accgataatc ataactccct
     7501 aaactaactt ttttcttttt aactaataca atagatatca ctttcatcat aatgactgat
     7561 gtttgattac tttgtacaga ttcacctaat tctttttcat ttgactttgc atcattaata
     7621 ctagttgtgt cttttctgtc gctttttcag ctatatcttg cctttacaaa tggtattcct
     7681 gagcgactag acgtctgaac gaacctattt gtttcaaatt ttgctttcct tccagttgaa
     7741 ttctctatta tactatcttt ttctaattcc cttttctgct gggattctca atttttgtag
     7801 gtaaaattgc cttattttct aatttactta gttgttaatt ttgatttaga ttttgttttt
     7861 ttatttgcac tttgttctat ctttttcttt ttattagttt tggttttatt aaaattttta
     7921 cctggtttac tcatttacta atatgtttag aaaacaaaca agatatagat atacagtact
     7981 aaaataactt atttctgtta taatagaaaa cctgttattt aaaattaaaa atagacgtat
     8041 aacaattctt tcaatagcat atactacttt caatagcatc tactatcatg attgaatgaa
     8101 aattaattat ttgaactttg ataataatat agtttcttat aaaaattaac tgtttcgttt
     8161 atttctcata aaattaatat gcaaaaatat tattattaat gtccgaatgc ctaatataag
     8221 tagtttaaat atgttttgta gtatattttt atttagaagt attaagctat ttttcaggga
     8281 acatattaat ttttatattt ttaaacgttt tgttaaaata tagatcatca atatttttta
     8341 cataatacac tagtacagaa taccattaat aaaattaaag tataaaaaat agtggtatat
     8401 tcttattagg attaataaca tttctcaatt atatgcaaaa acaaaataat ataacaaaaa
     8461 taagaaaagt tgaaaataat attaatcctt tatttgtaaa cagatggtct ccaagagcaa
     8521 tgtcaggtga agaaatttct cacgatgatc ttatggggct ttttgaagca gctcgatggg
     8581 cgccttcatc ttacaataac cagccttgga ggttcatata tgctaagaga aatactgatc
     8641 attggcaaag attattttca cttttagtag aagggaacaa aatttgggct aaaaatgctg
     8701 ctgtaatcgt cgttgttatt tcgagaaaga attttgaatt taatgagaaa ccagcaataa
     8761 ctcatcaatt tgatgcaggc gctgcctggg aaaatttggc tcttgaagcc actttacgag
     8821 aattagttac ccatggaatg caaggttttg actttaatga agctcgaaag gcgttaggaa
     8881 tcccagatag ttttgatgtt atggccatgt tcgcaattgg aaagttagga caaaaagaaa
     8941 atcttccaag agatcttcaa gaaagagaat atcccagtaa tagaaaacct ttagaagaaa
     9001 tagtaatgga gggtaaattt ataggaagat cgatacaata gaaaaatttc ttttttattt
     9061 gttcttgtaa accaactcta aactgcttag aatggtataa aaaagtgttc catgaaaaat
     9121 tatcctctat catctattga atctaaagtt ttcgttttct agaagattgt tgataatttg
     9181 aaagtatctg atatttaaat tgaaaaggaa aagaataaac aattgattat atcagtaaag
     9241 aaaataatat ttgttattta attatattat tatttagagg tatttacaaa tactgataga
     9301 catagaataa ctaatttttg ccccaaaata taataaaaac tatctagtat aacggtttaa
     9361 gaaatatata ttatgtataa tatattatta atttattaat taattataaa ttgctttaaa
     9421 tttattttct cattgtatat ttaattgtat tagccgaatt aaaaaagttt tcctcaacat
     9481 gctaagatta ggtaacataa aaataagatt tataatttta ttttcgatgc ttggcaaaaa
     9541 atatagtggt taattaggat tatcgagtga tgttccttgt ggttttatgg tagaagttac
     9601 attaccagca ttagttgttt gagaactatc ttcagacatt gaacttttgt tttcttcacc
     9661 tagtgatgtc ccttgtggtt ttatggtaga agttacatta ccagctttcg ttagctctgg
     9721 aggttgatct agttgtgcag ttacaggaat gattgtaata agagaagtat tgattaccac
     9781 catgagtata atggtaagac ccgtttcctt cataatagta attagtaaga aaatattata
     9841 taggttatgg tcttagtcag aataaattac tagtttagtt agttcatcat attaatttga
     9901 aaaacatgag ccatacgaca actagtattt tggaaacaga gaggatgttg tatatttagt
     9961 ataaatagaa aaacaaaata ccaaagaaat aactaaatct gctaaagata ttgttgttat
    10021 tcctcaattt ctgtagatgc ttcccttgtg agcctattat aattgttagg cgttctctaa
    10081 taagttcaag tttcctatat gatgatattt tccaccttaa tagaggaatc ttttcttgtt
    10141 gtttcttcca atttaaaact tttatcatat tctgatgtat tatctccttt tttaattgat
    10201 gtcatttcta ctggtagtat gtgttcatta aatattaaaa aaattgtatg actgttacac
    10261 tttgtgctgt aacaggagtt aattgctatt tatcgtttct tcttttgtat cctttaaacg
    10321 tagtattttt atcacactaa attttaaagt tatttctata gcaattattt ttctaaatat
    10381 atcattcgac ttgtatgata aatttataat tatttattac gtagcgatgt gtctttcttg
    10441 ttatgatgaa atatatggaa tttattatac caatataatg cacctgacat tattataaaa
    10501 tccgcaacat aaaatagctc ccaaacccat agattttctg aattaccgtt gacaaaatca
    10561 attgcatatc catcatcagc tatggcattt ataaacagag agagagaaga aaggaaccaa
    10621 ggaattgatt gttgataatt ctttcttaaa cttatcaaaa ttatcgcaga aggagcaatt
    10681 aatattaagt ctagaatagg atacataagg cttaccacta tagaaataaa attgtcattc
    10741 aagtcaaatt ctgaaaagag taaatttgtt acaacataaa ttacgaatat tatagtaact
    10801 attgatataa taatggcatg tataggattt atttcccttt gtaactcttt cataataaca
    10861 aataaatgta aagagagaaa tccatatccc ataaaataga atgcatctac aattgagaca
    10921 tcaattagtt cctttacgtc gataataaaa taataggtaa caataatgtc tgctgtaaac
    10981 cacaatataa tccctatagt aaggaacaaa tatgattttc catggatccc agaaaaccca
    11041 tatcttatta tagctataat tcctaaactc gatgcagtac ctgctgcgat atttaaaatc
    11101 aaaagaacga acgaatgtct atacttaaca tctagtaata gtgttatcaa attcactatc
    11161 attatagata taaacatcga taagatgaga tagcgcaatg gcgatgtatg ttcaatctgc
    11221 ttttttgagg acaaacaatg atttccttgt tattactcta agaaacactt tttattttat
    11281 tacattattt taatatagaa ccgtagttag tatagaataa aaaataatcg ctatggatta
    11341 aacctgtaca tattgcataa acctgaactg ttgcattata tggacagagt aaaatctttt
    11401 catattatca gattattttt tagtagtatg taaatattac ttttttatta gcaaaatcat
    11461 gtacattgtc aattttatgt cttagttgta ataataatga tattaataaa tagtatagat
    11521 ttaatctatt ttaatgttta aaaacctcca ctaattatat aatcacataa gaaaagtaat
    11581 ttgagattaa ttgtttatac agggaaaggc gggacaggaa agacggttac atcctgtgca
    11641 accgcgattg gtttggcaga aataggttat aagacacttt tgatatctgc agatcctgca
    11701 cataccgtta gtgatgcctt ttgtattgat ataggacacg atacgaaaac aatcagttca
    11761 aatcttgatg cgttagagat tgacccgatg gttgaaatta gtaaagacta tgttgatatt
    11821 atttcatcca taagctcttc ttttattggt aaaggagtga attcaagcat tgcttatgaa
    11881 atagctatgt tgccaggtat gactcaactt ttttctttga ttaaaatcga agaagttatc
    11941 agagaaaata attatgaaaa aattgttctt gatatgcctg cttcaggaga ggcattgaag
    12001 tttctttatt ttccaaaatt aatgggatct atggctagac atatgacagg attaacaaat
    12061 ctgtttagtg gatttgcgaa gatgtttcaa ccactatcaa gctattctac ttatttggat
    12121 tcatttataa ctcaaaccga tatactaaaa cgtcttgata atttatttag tattttatcc
    12181 aatcagacta tcacaagctt gcgtcttgtg actaatttaa ataactttag tgtaaaaaat
    12241 gctaaaagag cccttatgtc agctaacttg tttggaatta acgttgacct tgtcataata
    12301 aataaagtag aggttataga tgaagacaaa agaatatttt tagagaaaat caagttgaat
    12361 ttttttccgt taccttgtcg agaagcaata cgtatgaaag aagaactcca gggtattgaa
    12421 atgttaaaac aacataatta ccatattttt gggactgacg atccttctaa tattttcttt
    12481 actgagaaag tttattctat tgaagaaaat aatgagtata ttaccttgaa atttaaaatc
    12541 ccttttgcta ataatgccag tttggatatt caaaaaaaaa gtgacgagtt aatgataact
    12601 ttagaacatg atagaggaat tcttaccaat actataacct taccttttgc ggctgtaaca
    12661 atgaaaattg tttctagtga agtcgttaat gacaatttag tagtcatact aaaacgatag
    12721 ctataacaat attcttggta atgattttat tctttacgta aacgccggta ccacatctgt
    12781 tagataaatt tactttttat gagtttaaat atataattca taatccacta agaaggttct
    12841 aaattatata ttttaacgta aatagcaaaa aaggtataat tactaaaatt ctatggttaa
    12901 ttatccgcta taaacttcta attacgtttt aatctttgtg ctaaataaat tatgaaatca
    12961 acaatttgat acagtattta ctagactaac tattccctac tcgataatcg atatgaacac
    13021 cactaaacaa ttatttttta acattaaaac aatatattta ttattttcct atgaaatagt
    13081 gatctatgaa agtatcattt aatccaaaaa cgtaccttgg aaggatgact ataatcttca
    13141 tttctatttt cgctatttta ataatagtta gcacaattca tggtactatc cttatcaatg
    13201 cacaattaga ttccacttat gcattaaacg acactctatt tgttagtgga tcggctactg
    13261 ccaatacaaa agctgacaaa gttgttgtat ctttaggtgt agaaacaacg gataaaatgg
    13321 ctgagaaagc gttagtgtca aattctattt tgttgaatag agtgttagct gcattaaatg
    13381 aatctggtgt ccaacaaaat gaaacgagta cttattcatt ttctataaaa cctaattata
    13441 attattccaa ctatggagat agaggaaaat taactggttt cactgtatca cattctattc
    13501 atattgagag ttcaaatatt gatagtgtct cacagtggat agatacagca gttcaagctg
    13561 gtgccaatac tgttgatgat atttacttta gcgtatcaga agataagtta caaaaaataa
    13621 aaaatacgtt gctaaaagaa gctgtttcaa atgctaaatc gaaagctgac attgttgctg
    13681 ctgcagctgg attaaacgta gcgggaataa aatcgataac agtaggtgga gaaatgggct
    13741 tgcctccagt tccaggcccg ttatatacta aatcagttag ttctgatgaa gcatcttcaa
    13801 cgcctatact tgcagctgaa caagaggttt ctacgacagt aagtattgtt tatattgtag
    13861 gtagataatc attctttaac aattttttta ctaatatttt tcatttataa acattcaagt
    13921 atattgaaat tattttttct tgataaggtt ttgaatatat agatgacaat catacacaaa
    13981 gagatatata tatttaataa tttctaatca ctatttattt gttaatattt tgaacttttc
    14041 taatattcaa gtaaaaaatc atattataat atactcagat attgaaaagc atatagctat
    14101 gattaaataa aaaaactatt aattagaaaa aaatagtgtt gattttatct tagacatctg
    14161 aagtttgttt tataatcaat attatattat ttatcgtgtc atattgcgac attcttgtgc
    14221 acacattc
//
LOCUS       DASVEV010000048         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1177235, whole genome shotgun sequence.
ACCESSION   DASVEV010000048 DASVEV010000000
VERSION     DASVEV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177235"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1551
                     /locus_tag="VF222_02595"
     CDS             433..1551
                     /locus_tag="VF222_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7141347.1"
                     /translation="MRALIRNSKEKKVLNLSEYYLTKDNKMFYLPQINGNKTSIFIII
                     FMFIMIIPTTAIVLTTTITITVYGQQDKTNFNVTDSSNIQSIPAKKVQVGDIDIAYKM
                     LGKGDPILLYNGASDGMDAWDPSLLRILSSNHTVIAFDSRGLGNTTIGSKPYTYQQLA
                     TDAAGLLDALKIPKADVMGYSLGSYIAQQITIMYPDKVNTLTLVGSSCGGKDHTPKPS
                     EFLKLQSQIVNKSLNNISASQEDMKELVAASVGQGWIKLHPEVLDNIPTMQQAKPGLT
                     PEVMNNQNNVGKHWEDNPNWSGACDELAKLDKPTLVITGMDDNMYAPHVNSLKIAEKI
                     PGAWLVQIKNAGHAVPDQYPEEIGKILNTFLSTTAQTH"
     gene            1808..2146
                     /locus_tag="VF222_02600"
     CDS             1808..2146
                     /locus_tag="VF222_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141348.1"
                     /translation="MVAKLSTTINKIQNLSNSPNGKTLIEFLMYMKNNGSSERHQNNN
                     LIVMIEFSNYFDSNTAFYDIDKNEQILLFLDTKIKDSSKDPEKRWITSWNHYLVRIKL
                     FFRWLLHIFT"
     gene            complement(2129..2260)
                     /locus_tag="VF222_02605"
     CDS             complement(2129..2260)
                     /locus_tag="VF222_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141349.1"
                     /translation="MSEFELCPTCKKGKFRQTENNTNELVCDYCGEKCSDEGLSEYM"
     gene            2751..>2889
                     /locus_tag="VF222_02610"
     CDS             2751..>2889
                     /locus_tag="VF222_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141350.1"
                     /translation="MGNRSRTEIVAMILDAANEGGETKTKIMYFAFLSYNQLKEYLSI
                     LI"
BASE COUNT         1062 a          450 c          443 g          934 t
ORIGIN      
        1 ttctgtttta gttgaaaaaa acctaataga atatctggag ggggctaata aatataaaac
       61 aacagaaaaa ggattgttct ttcttaaaat gcataatgaa ttgggagaat tgttaccaca
      121 aactacaaca atcaaaaatt gactaattaa ttaaaagttt ttgtgataga gattaatata
      181 tatacgtata attttttata gtagttctat ttttgtatct atttaaaaat aagttctaat
      241 aataaaagct aaatatctgt tagtattatt cctacgatag tagactctta ttttagaacc
      301 gctatcggtt ttactcccga cgggccagta ctttttctcg ggctcaactg ttagaaaata
      361 tagaatatta ttagatatgt tcaaaatatg ttaacaataa gaagattaac ttgtatcaac
      421 ttttgatata ctatgcgtgc tttaataaga aattctaagg aaaagaaagt cttaaattta
      481 agtgaatatt atctaacaaa agataacaaa atgttttacc tcccacaaat taatggaaat
      541 aaaacttcaa ttttcatcat tatcttcatg tttataatga ttatccctac tactgctatc
      601 gtgttgacta ctactatcac cattactgtt tatggtcagc aagataagac aaatttcaat
      661 gtaacagatt catcaaacat acaaagtatt ccagcaaaaa aagttcaggt aggggatatt
      721 gacattgcat acaaaatgtt aggtaaaggt gaccctatct tactttacaa tggtgcttct
      781 gatggtatgg atgcctggga cccatccctt ctaagaattc tttcttcaaa tcacacagta
      841 attgcgtttg atagtcgagg acttggaaat acaacaattg gttccaaacc atacacttat
      901 cagcaattag caactgacgc agctggtctg cttgatgctc tgaaaatacc aaaagcagat
      961 gttatgggat attctcttgg atcgtatata gcccaacaga ttacaatcat gtatccagac
     1021 aaggttaaca ctcttacact tgttggttca tcatgtggtg gaaaagatca tacccccaaa
     1081 ccgtctgagt ttctaaaatt gcaatcccaa attgtaaaca aatctctaaa taatatttct
     1141 gcttctcagg aggatatgaa agagcttgta gctgcttcag taggacaggg atggattaaa
     1201 ctgcatcctg aagtacttga taacattcca actatgcagc aagcgaagcc agggcttacc
     1261 cctgaagtaa tgaataatca gaataacgta ggaaagcatt gggaggataa tccaaactgg
     1321 agtggtgctt gtgatgagct tgcgaaatta gacaaaccta ctttggtcat aactggaatg
     1381 gatgataata tgtatgcgcc gcatgttaat tctttaaaga ttgcagaaaa gattccagga
     1441 gcctggcttg tacagatcaa aaatgcgggt catgcagtac cggatcaata tccggaagag
     1501 atcggtaaga tattaaacac atttttatcg actacagcac aaactcacta ataatatatt
     1561 tattataatc tagcagtagt atgacgagtt agttgtatcc cttggaatga acaagtaaat
     1621 tagattcaat aacaaaagag ccccccaagg atcctttatt ggttctatcc cattttatgt
     1681 tattgtctca ttttgggcca aaaataaagt gtcaataaca attcacacac ctctgagccc
     1741 gttatgtata tgggttcata taattgagaa ttaaaataaa tcttaaagga agagggatta
     1801 ctccaagatg gtagccaagt tatctacaac tattaacaaa attcaaaatt tgtctaattc
     1861 tcctaacggt aaaacattaa tcgaatttct aatgtatatg aagaataatg gctcgtcaga
     1921 aagacatcag aataataatc taattgtaat gattgagttt tccaattact ttgattccaa
     1981 tactgcattt tatgatattg ataagaatga acagatacta ttatttttag atacaaagat
     2041 aaaggattca tcaaaagacc cagaaaaaag atggataaca agttggaacc attatttagt
     2101 aagaataaaa ttatttttta gatggctatt acatatattc acttagacct tcatcagaac
     2161 atttttcacc gcaataatca cataccaatt cattagtatt attctctgtt tgtctaaatt
     2221 tgcctttctt acatgttgga cataattcaa attcggacat atcatattaa atcattagct
     2281 agaataaaaa gttctatcaa ccaaattttg acactttagt tacaaatagc aatcaaccag
     2341 taaaatattt aaatatatta taatctaacc aagaattaga ttcccctttt agaaccgcta
     2401 tcggtattac tccctcattg agatatttct atcgaattca atactgtgaa tatctctaaa
     2461 aataagttct aataataaga gctaaataca ggatagtagt aaaaccatac tctatagaat
     2521 tctactctct attattttat acaaatagag ttatatttca aattaaatca tatagagaat
     2581 gattgttgat aaaattaaat atgataatta tttcataata caattctgag ataagatagg
     2641 aacatttctt atatttatta tttgcaaata atatgttgaa tgttttacta tagttaggtt
     2701 aacttttcct atgatgttat attaaaatag taaatcaatt atctaataat atgggaaata
     2761 gaagccgaac agaaatagta gcaatgatac tggatgctgc aaacgaagga ggagaaacca
     2821 agacaaaaat tatgtacttt gcttttctaa gttataacca attaaaagaa tatctttcta
     2881 tcttgatag
//
LOCUS       DASVEV010000049        11508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_11841, whole genome shotgun sequence.
ACCESSION   DASVEV010000049 DASVEV010000000
VERSION     DASVEV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11841"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..953)
                     /locus_tag="VF222_02615"
     CDS             complement(45..953)
                     /locus_tag="VF222_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7141351.1"
                     /translation="MNQLKLWIIIITLSLTVGSSFIAIKIVLDSIPPLFAFSIRFMIT
                     GGVLLLVSYIFDRKSQEIKKITFWRNALFIGVFLILGGHGLIAYGAQYLSAGIASLLN
                     STIPLWVALFMFLIFHNKITNLTKVGLALGFSGMIILVGPSIGGGAQGLSLVGVASLL
                     TSSLLWAVGSIFSSKASLPQNFLLSAGMVTLIGGSLLLIPSILTGELNLLPSFSDINS
                     NLLISYLFLIFIGTVIPLAEFYWLLKVSTAAVANTFAYMAPIVAVFLGWAILAESVTY
                     LTIIAIIVILIGVALIVRTSENSNNK"
     gene            complement(1211..2245)
                     /locus_tag="VF222_02620"
     CDS             complement(1211..2245)
                     /locus_tag="VF222_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141352.1"
                     /translation="MTKLIAGLQNSVLVIESSSKSGGWKTYESLKGSHPQSIAFDLLN
                     PSRAYCGTFDKGLLKTDNGGQTWDSIGKNEISTSSIMSVSVSPLEEGNNEFNKVYVGT
                     EPSALYISHDGGESWERMSGLNNLESSATWSFPPRPWTHHVRWIEPDVNNLNYVFVAI
                     EAGALVQSRDGGRTWIDRVKQGPYDTHTLATHRKMPKRLYSSAGDGYFESFDYGESWN
                     SQTTEGLRHHYLHGLAVDSSDPQNVIVSASLGARQAHNIENANSVLYRRSADGENWKI
                     VSKGLPESKGTIITMLASNPNNAGEFYALNNRGIFISDDSGISWVALEIPWPKEYLLQ
                     HPWSLAVRKE"
     gene            complement(2288..2851)
                     /locus_tag="VF222_02625"
     CDS             complement(2288..2851)
                     /locus_tag="VF222_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7141353.1"
                     /translation="MDLLWNDERSNQFITNVGLITSDGPFGPNVMACEWTHHISYKPG
                     LIAVSLGHTKATVENIRTSKEFGINLCASDQSVLASVAGGYTGAKYNKVNALKELGVE
                     FYKAKRITSLMIKGAVLNIECRLYKEITLGDHIMFVGEVIEASNNPDKVPLAYHGGKY
                     WIMNTNLVKPSTEERDRIKKVIEKYSI"
     gene            complement(2874..3242)
                     /locus_tag="VF222_02630"
     CDS             complement(2874..3242)
                     /locus_tag="VF222_02630"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX7141354.1"
                     /translation="MSKVIEIIEKEILSWPYVTVGSHRFGGIEFRLNKKEMGHIHSEG
                     LADIPFPMKTRDELVNSGLVKPHHVLPQSGWVSYWFHNSGEKEDIATVIDLFKLRYEL
                     LKPKAQTKSSNQKRYNSSSS"
     gene            3531..4208
                     /locus_tag="VF222_02635"
     CDS             3531..4208
                     /locus_tag="VF222_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141355.1"
                     /translation="MTIPANLENVSDGFSEPKKKILYYLKVMQQAGLEELAKVMKVSR
                     MAVHKHLVLLQNRGLVEGIENRGHVGRPRMVYQLTSQSKTVFPKSYSAIATHALDFIE
                     RNMGKEGVEKVLHERQTELFDQYYKRLKGLDFDKQVKELARIRDEEGYIAESKKESKR
                     SGGKHVLLEYNCPIIHIAEKHWEACSTETELFEKLLGANIETTHRAAKGDSICKFVIK
                     EKREEYF"
     gene            complement(4457..4774)
                     /locus_tag="VF222_02640"
     CDS             complement(4457..4774)
                     /locus_tag="VF222_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141356.1"
                     /translation="MVLDSLSTPTNITNLVITSENDSSVAVGRKELSLELEKKIYSIT
                     KGASSSAFFKYFKNMASANIQNTNILYDFIITEQNHTNVKLSTRISYIKVICLFNRYF
                     HKL"
     gene            complement(5143..6462)
                     /locus_tag="VF222_02645"
     CDS             complement(5143..6462)
                     /locus_tag="VF222_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141357.1"
                     /translation="MPYQQGIRLPGEKASRISHIDLLQNKLINEVIKKFELSETNLQS
                     LHPHWLALPSNSRALPYVFAVDGSYQIITIDEPHFWKVAFIKTALLMLDQNALSKIDK
                     DAPHPLQLRDIISKSAVYHGALFPLKNIKYSGKSIYHAIRQNIYDSIQDKSSPLFGEP
                     METLKWIVYKKWKNNQEGLPRFQCPHCEKIVATLPYNSEIGNCPVCKEQIFITDMLGF
                     HQEMAEDFAPDQLVSNYMNVHELLLLFTGIRYFWQTNKDVIENSLFVKDGPLMMPSQY
                     SKLVEPIRQFIEFAQQKGYNINIIGQEKSGAFFEHLQLIGKDAPLKTMFIPNDSYIKE
                     EIQQRPKKQFQHLYGEKTNYGAKVFIKLNNYHQMILNIPTGRFNPNPHISDLIGIDRI
                     CSTLETILSYRYEGALLPIELVHGIASLSTYPSAEILKIFAKASTGS"
     gene            complement(6464..6721)
                     /locus_tag="VF222_02650"
     CDS             complement(6464..6721)
                     /locus_tag="VF222_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141358.1"
                     /translation="MVYSTQSVTSIHADLLNQTENFFILHLSSQYEVQALSKINFAFD
                     KFQNDILQSKTPGYARILTRSHKFVVPVQANKFKPIQKEAS"
     gene            complement(6878..8461)
                     /locus_tag="VF222_02655"
     CDS             complement(6878..8461)
                     /locus_tag="VF222_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7141359.1"
                     /translation="MQTSIKQNHPLGILSSFLENEKMKEQSANYKESNFVGYAIKVTY
                     DTVTVITSEPFKLAVGGVSRNSLLVMVPINIESTTPHFTLLRVIQSAPTPLDQYQQET
                     YFALHKNSMPVLDPFTNSDLQWGALETNVLGMFYPSPEKNDVIEFSHDMNNFVSAHKY
                     RIYYPNDELLDFIINSLVSDVNKFNIGTLRLTECRLQLPNRPLPNVSVNISTKDFMGT
                     RTAMFGKTRFGKSNIIKLIAQSIMETTKKSHNVGQLIFDIDGEYANDNPQDDSGSIRS
                     IYQDDCTVYALSQKKNTPSKQLRLNFYEHPDASHRILSNLLDKNKRYSAVYIKNFSEV
                     EIPSLASITEMDIHEQIRNRRKILLYWAILYKAGYDIDETKLGERFPSGFDPGYNDKL
                     RKAIHGDNPPEIKSLANLLREYELISIFLRKRERRRGGEGDEQENDVLSKSGRPLFET
                     DERSLLEFLEPPYGRSGTRTIQEFRRYHAKNAGDFVKEILNLLDVGKTIILDLSNSDT
                     EVTRYFSTDLYEAIYQHQV"
     gene            8548..9573
                     /locus_tag="VF222_02660"
     CDS             8548..9573
                     /locus_tag="VF222_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141360.1"
                     /translation="MQKKFTDPGEAAKQLLPLIAEKICFSTYQCYKELNKQNKISYKN
                     VHKKVKKLHEVGILKEVSNNGKHASIYYKLSSFGIYFIFLTHKINSFDLAKLIRNYPN
                     DGLFEYFLYQFIERKTIKEIKANSIFESIFQFLNECCSSIEYFLKTLSGIEKRGGSSV
                     HITITDELIDPDLEDHWYGGSQVFIDYLKTKFKIKWLNKNTSQIKFNKSDNLIEIVEK
                     KDSLILKLNPKIEKAILSYENNILFEFEIEELEKGCFAINEFQPSTVREQKYSDHKIY
                     ESGSTLCMRILNYADYEVGAGKLEDFEMITSLKWLSKDQRLRNTLQQIKNKFDNKFYN
                     FERWSLI"
     gene            9848..10630
                     /locus_tag="VF222_02665"
     CDS             9848..10630
                     /locus_tag="VF222_02665"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141361.1"
                     /translation="MNEKESIKCIQKQITEIKTFINEGPYSTKHTKWISDTQFLLEDI
                     FGQNSRIFMTFASLNWNIPPGSTFVVEAPFMDHEIQRRENIGFIKDLKISKGLLLSGI
                     EQIKRLGIKNVYNGKNTPKESSDIIKIISLIEKKLRKTVRNKPIIEKEIQDKLQDLFI
                     GADLDKKFTREKERVTYSSKTYIPDFYFDKINTVVEVKLCNKYEKEKVIISQINDDIM
                     AYKTKYDNIIFVIYDLGVIRDIDEFQGIFESNENVILKIIKH"
     gene            10822..>11508
                     /locus_tag="VF222_02670"
     CDS             10822..>11508
                     /locus_tag="VF222_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141362.1"
                     /translation="MSKNLQNNLLLCISNALYEINEILGYEYFESTNIDNLSKKFLKM
                     LPYSEKLLQQVRGVSLKDIEKEEMDIDKGMKAVIKGIFHGVIIKNFTGKTFEEKFLNF
                     LYYDGITHCRWINKTDYHNYYNTLFTLVKTINSDLEKLKFIHNLLEIKRNSTSQGITK
                     IFLKKDFFKEYSNHNELTIDLYIEVFKELAGIIESYIKLIYGIKANYTTNDIIRLNKK
                     KFYYFWEHLKN"
BASE COUNT         4077 a         1722 c         1784 g         3925 t
ORIGIN      
        1 agattcattg tttcgttata gattgttttg tcaaagattg acaatcactt gttattacta
       61 ttttctgatg tcctaactat caatgcaaca cctatcaaaa taacaattat agcaataata
      121 gttagatagg tgacagattc agctagaatt gcccatccca aaaagactgc tacaataggt
      181 gccatataag caaaagtatt agcaacagct gcagtactaa ctttgagcag ccaataaaat
      241 tcagctagtg gtataacagt gccaataaat attaaaaata ggtaggatat cagtaagtta
      301 gagtttatat ctgaaaaaga aggaagaaga tttagttctc cagttaaaat actaggaatt
      361 agtagtagtg atccgccgat taatgtaacc attcctgcgg ataataaaaa attttgaggc
      421 aatgatgctt tactagaaaa gatagacccc actgcccaaa gcagtgaact tgttaacaat
      481 gatgcaactc ctactagact taatccttga gctcctcctc caatcgacgg tccaacgaga
      541 attatcattc cgctaaaacc aagagcaaga cctacctttg ttaaatttgt tattttattg
      601 tgaaatatca gaaacataaa taacgctacc cataaaggaa ttgttgaatt cagtaaagaa
      661 gcaattcctg cagagagata ttgagctcca tatgctatta atccatgtcc accaagtatc
      721 agaaagacac caataaacaa agcatttcgc caaaatgtaa ttttttttat ctcttgactt
      781 tttctatcaa aaatataaga aactagtaat aaaacaccac ctgtaatcat aaatctaata
      841 ctaaaagcaa acaacggtgg tatagaatca agaacaattt tgatagctat gaaacttgaa
      901 ccaacagtta aagataaggt aataattatt atccataact ttaactggtt catagataca
      961 tatttccata aatcatttta cttatatctc tctccgttat tgctttagaa ttactatgaa
     1021 atagaaataa tctcttttgt aggatcaact ctacatcaaa ataatttaca gaataattat
     1081 gtatcctacg acttagataa tgatcttcca attaataggt actctagtca ataagttcta
     1141 agatgtatat ttatttccca tgaaataccg ggatagattg agctcattcg atcttattct
     1201 gttcatacat ctattctttt ctgactgcta gggaccaagg atgttgcaaa agatattctt
     1261 tgggccaggg gatttcaagc gctacccaag aaatacctga atcatcagag ataaatattc
     1321 cacgattgtt gagagcataa aattctccag cattatttgg gtttgaggca agcattgtaa
     1381 ttattgttcc tttggattca ggaagacctt ttgaaacaat cttccagttt tcgccatctg
     1441 ccgatcttct gtataataca gagttggcat tttcaatatt atgtgcttgc cgagccccta
     1501 gagaagccga tacaatcaca ttttggggat cagatgaatc tacagcaagt ccatggagat
     1561 aatgatgtct aagtccttct gttgtttgac tattccaaga ttctccataa tcaaagcttt
     1621 caaaataacc atcacctgca gaagagtaca gccgcttagg cattttccta tgagttgcca
     1681 aagtgtgagt atcataagga ccttgcttca cccggtctat ccatgttctg ccaccatcac
     1741 gactctgaac tagagctcct gcttctatgg caacaaaaac atagtttaaa ttatttacat
     1801 ctggctcaat ccaacgaaca tgatgagtcc aaggtctagg aggaaagctc catgtagcag
     1861 atgattccaa attgttgaga ccactcattc tttcccaaga ttcacctccg tcatgtgata
     1921 tataaagtgc gcttggctca gttccaacat atactttatt gaattcatta tttccctctt
     1981 ccagaggact gactgaaaca gacattatac tagacgtgga aatttcattc tttccaatac
     2041 tatcccaagt ttgcccacca ttatcagttt tcaataaacc cttgtcaaat gttccacaat
     2101 atgctctact tggattcaaa agatcgaatg ctatactttg tggatgactt cctttcaaac
     2161 tttcataagt cttccatcca ccactctttg aagaagattc aatcacaaga actgaatttt
     2221 gcagtccagc tatgagctta gtcattaaac gaaagaatca cctacttttt gtgtgcatca
     2281 tcttgtatca tattgaatat ttttcaatta ctttttttat tctatcgcgt tcttccgtag
     2341 atggctttac taaatttgta ttcatgatcc aatactttcc tccatgatat gcaagtggta
     2401 ctttgtcagg attattggaa gcctcaataa cttcgccaac aaacataata tgatcaccta
     2461 gagtaatttc tttatataat ctacattcaa tatttagtac agcacctttt atcataagag
     2521 aggttattct ttttgctttg taaaattcaa ctccaagctc ctttaatgca ttcaccttgt
     2581 tgtacttagc accggtgtaa ccacctgcta cactagctaa tacagattga tcacttgcgc
     2641 ataagttaat accaaattct tttgacgttc tgatattttc aactgtagcc tttgtatgac
     2701 ccagagaaac agctattaaa ccaggcttgt aggatatatg atgtgtccat tcacaggcca
     2761 tcacgtttgg tccaaaagga ccatcgcttg ttatcaaacc tacatttgtt ataaattggt
     2821 ttgatctttc atcattccac agtaaatcca agagatatca ccactaattt tgtttaggag
     2881 gaactactgt tatatcgctt ttggtttgag cttttggttt gagcttttgg tttgagcagt
     2941 tcatatcgca atttaaatag gtcgattact gttgcgatat cctctttttc gccgctatta
     3001 tgaaaccagt agctaaccca ccctgattgt ggtagtacat gatgaggttt aacaagtcca
     3061 gagttaacca gctcatctcg agtcttcata ggaaatggaa tatcagctaa accttcacta
     3121 tgtatatgtc ccatttcttt cttgttaaga cgaaattcta tacccccaaa tctatgtgat
     3181 ccaacagtaa cataaggcca actcaatatc tctttttcaa taatttctat aactttactc
     3241 atatagagat tatatttctt tctatgttta taaacatgga ggtttacaaa ttatattaat
     3301 tctctgttaa tatataagaa tctgaaagat ttgaacctgc aactactaaa tagaaagagt
     3361 agttttaatt atgaatatgt gtctaaaggt ttagagaaca tgctacggag tatagtagtt
     3421 ggctagcatt ttaatcaaaa ggaaatcaac aataacaact cgaacattga ttcaatttgt
     3481 aaacaaaagg gtttatgaat gagttagccg gtaatatgta ataataataa ttgaccatcc
     3541 cagctaattt agaaaatgtt tcagatggat ttagcgagcc aaagaagaag atactttatt
     3601 atctcaaggt tatgcagcaa gcaggacttg aagaattggc aaaggttatg aaggtatcca
     3661 ggatggctgt tcacaagcat cttgttcttt tgcagaatag aggtttagta gaaggtatag
     3721 aaaatagggg acatgtaggc aggcctagga tggtgtatca actaacaagt caaagtaaaa
     3781 ctgtattccc aaaatcatat agtgcaatag caacacatgc tcttgatttt attgaaagaa
     3841 atatgggaaa agaaggtgtt gaaaaggttt tacacgaacg ccagactgag cttttcgatc
     3901 aatactataa gcgtcttaag ggtttagatt tcgataaaca agtcaaggaa ttagctagga
     3961 ttagagatga ggaaggatat attgctgaat caaagaagga gtcaaaaagg agtggaggaa
     4021 aacatgttct tttagaatat aattgtccaa ttatacacat agctgaaaaa cattgggaag
     4081 catgttcaac tgaaactgaa ttatttgaaa aactccttgg agctaatata gaaacaacac
     4141 atagggctgc caaaggtgat tcaatctgta agtttgtaat caaagaaaaa agggaagaat
     4201 acttttaaaa ttactttgct gaaagtaaca atcttatatt ctttctaaaa aaacttaatg
     4261 taatcttcac aagtatttta ccaaaattat ctcctttgaa aagtctaaag aagctttgat
     4321 gcattctcta tttcttctaa catggtttcc tttgacttga tccttccttc cttccttcgc
     4381 caatccattt ctcgaaacag tatgtgatat agcatatcaa ttaaacatca atgaatccat
     4441 aactataact gatttattat aatttatgaa aatatctatt aaatagacaa atcactttga
     4501 tatatgatat tcttgtagaa agtttaacat tagtatgatt ttgttcagtt attataaaat
     4561 cataaaggat atttgtattt tgtatattag ctgaagccat atttttaaag tatttaaaaa
     4621 aggctgaaga agaagctcct ttggtaatcg aataaatctt tttttctagt tctaagctta
     4681 gttctttcct tcctactgct accgatgaat cattttctga tgtaataact aaattagtaa
     4741 tattggtagg agtagatagt gaatcaagaa ccaatctttg tctatctcaa attatttgct
     4801 ttcattaata aagaatatgc acgtttccat gactagcatg gaggggatgg gatttgaacc
     4861 cgttacttgc attcacgggt ttatacaatt tataaattta agaaattgtc atatcgagat
     4921 ttctaaatgt tccacaattg gaaagataag gagctgattt acaaaagaag attgaggaga
     4981 ttcagatcat agatcagtaa ttgagagaga aaacgataat gaaaagaaat acgattgtca
     5041 atattagtag atcattcatt aacaatcgaa ggaaggtact gtttaagctt acgagtagac
     5101 catatccata agtattatag aaataattat agatatgtgt gtctaagatc cagtactagc
     5161 tttagcaaaa attttcaaga tttctgctga tggataagta gataaagatg ctattccatg
     5221 aactagttct attggtaaaa gagctccctc atatctataa cttaatattg tctctaatgt
     5281 tgaacaaatt cgatctatcc caattaagtc tgaaatatga gggtttggat taaatcgacc
     5341 tgttggaata tttaagatca tttgatgata attatttaat ttaataaaca cctttgctcc
     5401 ataatttgtt ttttcaccat atagatgttg aaattgtttt ttaggtcgtt gttgtatttc
     5461 ttctttgatg tatgaatcat taggaataaa catagttttt aatggtgcat cttttccaat
     5521 cagttgtagg tgttcaaaga aggctccact cttttcttga cctatgatat ttatattata
     5581 tcctttttgt tgtgcaaatt ctataaactg tcttattggt tccactaatt tagaatattg
     5641 tgatggcatc attaatggac catcttttac aaaaagtgaa ttttctatta catctttgtt
     5701 tgtctgccaa aaatatcgta ttcctgtaaa taataatagt aattcatgta cattcatata
     5761 attactaact agttggtctg gtgcgaaatc ttctgccatt tcttggtgaa atcctaacat
     5821 atctgtaata aagatttgtt ccttacatac aggacaattt cctatttcgg aattgtaggg
     5881 aagtgtagct acaatcttct cacaatgtgg acattgaaac cgcggaagtc cttcttgatt
     5941 atttttccat tttttataaa caatccattt tagggtttcc attggttcac caaagagagg
     6001 actagactta tcctgaatgg aatcataaat attttgtcta atagcgtggt aaattgattt
     6061 tcccgaatac ttaatatttt ttaaaggaaa aagggcacca tgataaactg ccgatttaga
     6121 tataatatct cgaagttgca atggatgtgg agcatctttg tctattttgg ataaagcatt
     6181 ttgatctaac atcaaaagag ctgttttgat aaaagctact ttccagaaat gaggttcatc
     6241 aatcgtaatt atttgataag aaccatcgac agcaaaaaca tacggtaaag ctctactgtt
     6301 agatggaagt gctaaccaat gaggatgtag agattgaaga ttagtttcac ttaattcaaa
     6361 tttttttatt acttcattta tgagtttatt ttgaagtaag tcaatatgag aaattctgct
     6421 tgccttttct cctggtagtc taattccttg ttgatacggc atattaacta gcttcctttt
     6481 ggattggttt aaatttatta gcctggactg gcactacaaa tttatgtgac cgcgttaaaa
     6541 ttcttgcata accgggggtc ttactttgta agatatcatt ctgaaattta tcaaaagcaa
     6601 aattaatttt agataaagct tgtacttcat actgagagga taaatgtagt ataaaaaagt
     6661 tttctgtttg attcaaaaga tcagcatgaa tactagtgac tgattgcgta gaatagacca
     6721 ttcctatatt acattttgct ccttcttttg caatttgtgt atatatatcc tctttaactt
     6781 ctttagctgg aaatagatga tgtgcttctt cgaaataaag ctgaatataa tgattaatac
     6841 catcatcatc tcttaatagc gaattactgg taaattttta tacttgatgt tgatatattg
     6901 cttcatataa atctgtagaa aaatatcttg ttacttctgt gtcggaatta ctaagatcca
     6961 gaattatagt ttttccaaca tctaacaaat taagaatttc ttttacaaaa tcacctgcat
     7021 ttttagcatg atatcttcta aattcttgaa ttgttctagt acccgatctt ccataaggag
     7081 gctctaaaaa ttcaagtaag gatctttcat cagtttcaaa aagaggtctt ccactcttag
     7141 atagtacatc attttcttgt tcatctcctt ctcctcctcg tctacgttcc ctttttcgaa
     7201 gaaatataga tattagctca tattctctta gcaaatttgc taatgactta atttccggag
     7261 gattatctcc atgaatagct ttacgtaatt tatcattata tccaggatcg aatccacttg
     7321 gaaatctttc tcctaatttg gtttcatcga tgtcataacc tgctttataa agtatagccc
     7381 aataaagtaa aattttcctt ctatttctta tttgttcatg aatgtccatc tcagtaatac
     7441 ttgccaaaga tggaatttct acttctgaaa agtttttgat atatacagca gaataccgtt
     7501 tatttttatc caacaaatta gataggattc tatgtgatgc atcgggatgt tcgtaaaagt
     7561 taagcctaag ttgttttgat ggagtatttt tcttttgtga aagagcataa acagtacaat
     7621 cgtcttgata tatgcttctt attgaaccac tgtcatcttg aggattgtca ttagcatatt
     7681 ccccatctat atcaaagata agttgcccaa cattatgtga tttttttgta gtttccatta
     7741 tactttgagc tattaattta attatgttac ttttaccaaa ccttgtttta ccaaacatag
     7801 cagttcttgt tcccataaaa tcttttgttg atatattaac agatacatta ggtaatggtc
     7861 tgtttggtaa ttggagccta cattcagtta gacgaagtgt accaatatta aatttattaa
     7921 catctgaaac taaagaattt ataataaaat ctaatagttc atcatttgga taatagatac
     7981 gatatttatg agcgctaaca aaattattca tatcatgtga aaattctata acatcatttt
     8041 tttcaggact aggataaaac attcctaaaa cattcgtttc taaagccccc cattgaagat
     8101 cactatttgt aaaaggatct aatacaggca ttgagttttt atgaagtgca aaataggttt
     8161 cttgttgata ttggtctaat ggagttggtg cagattgaat tactcgtaaa agagtaaaat
     8221 gaggagttgt actttcaata ttgattggca ccataacaag aagagaattt cgagaaactc
     8281 ctcctactgc gagtttaaat ggctcagaag taatgacagt cactgtatca tatgttactt
     8341 ttattgcata tcccacaaaa ttcgattctt tataattggc tgattgttct ttcatttttt
     8401 cattttcaag gaaggaagat agaattccaa gaggatgatt ttgcttaata cttgtttgca
     8461 tgattacttt attgagaaag attatattaa acatattcct ttcagttcca tatggaccat
     8521 tcagggtata tattagaatt atatgaaatg caaaaaaaat tcacagatcc aggagaagct
     8581 gcaaaacaat tacttcctct aattgcagaa aaaatttgtt tttctactta tcaatgttat
     8641 aaagagctaa acaagcaaaa caaaatttct tataaaaatg tacataaaaa ggtaaaaaaa
     8701 cttcatgagg ttggaatatt aaaagaagtt tctaataatg gtaaacatgc atctatttat
     8761 tacaagttat cctctttcgg aatttatttt atatttttga ctcataaaat taactcattt
     8821 gaccttgcaa aattaattag aaattatcca aatgatggat tattcgaata ttttctatat
     8881 caatttatag agagaaaaac gattaaagaa ataaaggcta atagtatttt tgaaagtatt
     8941 tttcaattct taaatgaatg ttgttctagt atagaatatt ttttgaaaac tctttcaggc
     9001 atagaaaaac gtggaggttc ttcagtgcac attacaataa ctgatgagtt aattgatcca
     9061 gatctggagg atcattggta tggaggttca caagtattta tagattattt aaaaacaaag
     9121 tttaaaatca aatggcttaa taaaaatact tcacaaataa aatttaataa atctgataat
     9181 ttgatagaaa ttgtagaaaa aaaagatagt ctaatattaa aactgaatcc taaaattgaa
     9241 aaagctattc tatcctatga aaacaatata ttatttgaat ttgagataga agaattggaa
     9301 aagggctgct ttgctattaa tgaatttcag ccaagtacag taagagaaca aaaatattcc
     9361 gatcataaga tatatgaaag tggttctact ttatgtatga ggatattaaa ctatgctgat
     9421 tatgaagtcg gagcaggtaa gttagaagat tttgaaatga tcactagttt aaaatggtta
     9481 tctaaagatc aaagattgag gaatacttta caacagataa aaaataaatt tgataataaa
     9541 ttttataact ttgagagatg gtctttaatt tgatatacta tgaattaaag atatgattag
     9601 cttatcatga atattataaa tgtaggttct gttgaacctt cggatattcc atttgggtgt
     9661 tgaaaagtgg atgaaaaatt aaacgtggaa agggattcga acccataagg tcacttgggg
     9721 ggttcttcct tttataatca atgaaaataa gttaatgaaa taaaaagaaa tcgtagatga
     9781 ttagatcgaa attacattta acataagaat aatttattag atttaataaa atagaaacca
     9841 attaacaatg aatgaaaagg aatcaatcaa atgcatacaa aaacaaatta cagaaataaa
     9901 aacattcatt aatgaaggtc cttattcaac gaaacataca aaatggatta gtgatactca
     9961 atttctctta gaagatatat ttggacaaaa ttctagaata tttatgactt ttgcttctct
    10021 taattggaat atacctcctg gcagtacatt tgttgttgaa gcaccattta tggatcatga
    10081 aatacaaaga agggagaata ttggattcat aaaagattta aaaatttcaa agggattact
    10141 tttatcaggt attgaacaga taaaaagatt aggcatcaaa aatgtttaca atggaaaaaa
    10201 tactccaaaa gaatcgagtg atataataaa aattatttcc ttgatagaga agaaattaag
    10261 aaaaacagta aggaacaaac caataattga aaaagaaatt caggataaat tacaagatct
    10321 ttttatagga gctgatcttg ataaaaaatt tacacgagaa aaagaaagag taacttattc
    10381 ttcaaaaacc tatattcctg atttctattt tgataaaata aacactgttg tagaagtaaa
    10441 attatgtaat aagtatgaaa aagagaaagt aataatttct caaataaatg atgatataat
    10501 ggcatataaa accaaatacg ataatattat atttgtaata tatgatctag gtgttataag
    10561 agatatagat gaatttcaag gaatttttga atctaatgaa aatgttattt taaaaattat
    10621 aaaacattaa ttatacatat taaaaaatct atattatatc tatacaacat ctaatttgta
    10681 gctatttact cataaatttt gaatgttaat agaattaaat cataaaaggc aatagtttaa
    10741 tgattatgag attgaaagat tgattgataa aatgtctata tttaaatatt aaaatgacag
    10801 tcctgaatta tttaataaag aatgtctaaa aatttacaaa ataatctttt actttgtata
    10861 tctaatgcat tatatgaaat taatgaaatt ttgggatatg aatattttga aagcacaaat
    10921 atagataatt taagtaaaaa atttcttaaa atgcttccat attcagaaaa attacttcaa
    10981 caggtacgag gagtatctct taaagatatt gaaaaagaag aaatggatat tgataagggc
    11041 atgaaagcag taataaaagg aatatttcat ggtgtaataa taaaaaattt tacggggaaa
    11101 acttttgaag aaaaatttct aaattttctt tattacgatg gcataactca ttgtagatgg
    11161 ataaataaaa ctgactacca caattattat aatacattat ttacgttagt taaaactatt
    11221 aattcagatt tagaaaaatt aaaatttatt cataacttac ttgaaataaa aagaaattct
    11281 acatctcaag gaattacgaa aattttcctt aaaaaagatt tttttaaaga atattccaat
    11341 cacaatgaat taacaattga tttgtatata gaagttttta aagaattagc tggaattata
    11401 gaaagttaca ttaaattaat ttatggaata aaagctaatt acacaactaa tgacattatt
    11461 agactaaaca aaaagaaatt ctattacttt tgggaacatc ttaaaaat
//
LOCUS       DASVEV010000050         9001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1177497, whole genome shotgun sequence.
ACCESSION   DASVEV010000050 DASVEV010000000
VERSION     DASVEV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177497"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..301
                     /locus_tag="VF222_02675"
     CDS             107..301
                     /locus_tag="VF222_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141363.1"
                     /translation="MSVITCRNCGADMVVLQPDRIHTIVDRKVLNWKDYVKFTIKCKK
                     CAISNILYWSKPKDNLGSGN"
     gene            complement(460..1056)
                     /locus_tag="VF222_02680"
     CDS             complement(460..1056)
                     /locus_tag="VF222_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141364.1"
                     /translation="MSTSIDFTELIKDRKGVITKDNQSCGNIIGEDEEKIIIEDGAVR
                     QHFYRVPKSAVGAYNGAELTLNISYDELKTYEDKDEDKDEDKDKDKGMLESITDSVKD
                     KTKSLKEKTVDKIHHDDDDDHKDHKKGNRFDQDIQTSTDLNNLNRENQGKELNRNIDS
                     GEKSDETSTNVDSSFSCEKCNTKFTSRQELKEHFSSQH"
     gene            complement(1859..2053)
                     /locus_tag="VF222_02685"
     CDS             complement(1859..2053)
                     /locus_tag="VF222_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX7141365.1"
                     /translation="MTRLVRKEDRGPMEVKVGNESKWICMCGLSNNQPFCDGRHKKTK
                     DEQEGKVYQYNPDGSRTEIQ"
     gene            2392..3144
                     /locus_tag="VF222_02690"
     CDS             2392..3144
                     /locus_tag="VF222_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7141366.1"
                     /translation="MFKTIFKKFSSSTNKKNIDPVLHGHKNYEDKLFKSIVGFSDIKQ
                     FLMRCIISKEQVHLLFTGPPATSKTVFLLEMFRELSNSYFIDSTATSGIGIVDFLFSN
                     PNTKFLLIDEIDKLTKKDQTVLYNLMETGILTEMKAEKTKGFRQQKMNIKIFATCNEL
                     TKLTQPLKSRFMKLELPEYTWEEFLTISNNLIKSRYKYLDERVSTRIAEVVWNDINTK
                     DIRDVLQIARLTRNLEDIEVIAHTLMKYKPKY"
     gene            complement(3658..3999)
                     /locus_tag="VF222_02695"
     CDS             complement(3658..3999)
                     /locus_tag="VF222_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141367.1"
                     /translation="MTVASIPFKNNGLAFLIAIIAGIFGFCGIGHIYIGKVLRGVLIM
                     FIGWVPFGIGILVFLFEFISQNDTLMPGLDLSMIFGIIYFGIWIIQALDANRLAKKYN
                     KYLMSNSKPLW"
     gene            4475..4846
                     /locus_tag="VF222_02700"
     CDS             4475..4846
                     /locus_tag="VF222_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141368.1"
                     /translation="MHPKNQVLIILFSIILIGLIGIPFGNPNFIINAIILELSFIILA
                     ILIAKGIGNTKPLYVCIFLALLIIIGNSLVTAHINRMITFSKPLNTIVLIIGGYLLQG
                     LLIYSSILTIKRRKLKNRQSY"
     gene            complement(4954..5313)
                     /locus_tag="VF222_02705"
     CDS             complement(4954..5313)
                     /locus_tag="VF222_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141369.1"
                     /translation="MSRSSIVVVDDESDLVFMFKVTLEMNGYSVIGFTNPIEALEYIK
                     KNHDKCTLLLTDYRMPEINGCELGTKIKEIDEKIKVIIITAYENIIEDPSDFELIKKP
                     ISTTKLLEVVANNINLK"
     gene            5576..7306
                     /locus_tag="VF222_02710"
     CDS             5576..7306
                     /locus_tag="VF222_02710"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7141370.1"
                     /translation="MFVETCEKIRSTTKKNEKIDIISKYIVNLDETSLSIAVLFLSGR
                     VFPIGSTLTLNIGFSTIMESLSEIALLDIKDIQNIHLKHGDIGAIAEYAVSKKHIISL
                     FNQQERLSLSYLYPQFKKIASISGSGSNKTKKNILKGLLIACSPLESKYLIKIITGEM
                     RIGSVEGLVEIALSRSFDRELNYIREAMLISGDISQVAVLAKKNILHSAKMKPFVPVS
                     FMLADVMFSAEEIINYYSKPLICEYKYDGIRLQMHKFDNEVRLFSRNLVDITYAFPEL
                     VKAAIKSTIRTPDTTLQNQVDFILDGELIAFKNDRPLHFQELQKRLRRKNVTDDIQTE
                     IPIYYIVYDIMYFKNNQVLKKSLLDRKNILSTISFKEPIINSSYKILDSIEQVIAIFN
                     ESKDMGHEGLVVKDPLSQYHPGKRGRYWMKLKKELDTIDAVIVIAEYGHGKRAGVLSD
                     YTFAVRDDDKKNHNDLNINNHLENIRLKTIGKAYSGLTDKEIGEMTERLREIIVEDNS
                     NRILVKPEIVLEVAFDTIQKSDRHNSGFALRFPRIKNIRIDKSLKDIDTLEKVRQIYK
                     NQIYVKHKEI"
     gene            complement(7444..7806)
                     /locus_tag="VF222_02715"
     CDS             complement(7444..7806)
                     /locus_tag="VF222_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141371.1"
                     /translation="MIINFAGDIDLANNIYNYFISNSIIKEKIEIKIEEDNIVLSLYN
                     EQDKKVIQQIRSLLQEYLDSFKIYKNHKIIELENILTVGIPKDIVEISNLVFCEICGY
                     GMSSEEELLVHRRSHGIL"
     gene            complement(8321..8902)
                     /locus_tag="VF222_02720"
     CDS             complement(8321..8902)
                     /locus_tag="VF222_02720"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEX7141372.1"
                     /translation="MVINNRITVLVCIFSSLVVILLSTPNIQGIFNSESSEEFKTYEN
                     PLYGINIKYPGDWRYYENNGPENFSPDRIFQVSFLSPSNKVLSDMVFVWFNIEKIKDS
                     LSLDDRRNILLKNLNNSSVDVKVQSTKLSGINAFMVDYTNKAVELQRNIGIEAISNGL
                     LYSLDFTAQPDTFERNMNSIETMIKSMNISSIA"
BASE COUNT         3306 a         1166 c         1150 g         3379 t
ORIGIN      
        1 tggtttacta ttttgctgtt ttaaacaata taactattca aatggttgaa taggtgtatt
       61 ttaacataac aaaatttaga tagttagaac accaaaaagt tattcaatga gtgttataac
      121 gtgtaggaat tgcggtgcag acatggttgt tttgcaacct gatagaattc atacaatcgt
      181 agatagaaaa gttttgaatt ggaaggatta tgttaaattt acaattaaat gtaaaaaatg
      241 tgctatttcc aacatactgt attggagtaa accaaaagat aacttaggat caggaaatta
      301 aacgttaata aattgaacaa agagaattct agatataggc taaatagtat atttgtatat
      361 ttatgtaaaa tattataata tgaaaataag attgacaata attaataaaa aaattagatt
      421 aaatttaact aaaaaactag ataatcaaaa aaaatgaaat taatgttgag atgaaaaatg
      481 ctcctttagt tcttgacggc ttgtaaattt tgtattacac ttttcacaac tgaatgaaga
      541 atctacattt gtagaagttt catctgattt ttcaccacta tctatgtttc tattcagttc
      601 ctttccttga ttttctctat ttagattgtt tagatctgtc gaagtttgta tatcttgatc
      661 aaacctgttc ccttttttat gatctttatg gtcatcatca tcatcatggt ggattttgtc
      721 aacagtcttt tcttttaacg attttgtttt atctttaact gagtctgtga ttgattccaa
      781 cattccttta tccttatctt tatcctcatc tttatcctca tctttatcct catacgtttt
      841 taattcatca tatgaaatgt ttaatgttaa ttcagcacca ttgtaggctc ctactgctga
      901 ttttggaacc ctgtaaaaat gttgacgtac tgcaccatct tcgattatta ttttttcttc
      961 atcttctccg attatatttc cacaagattg gttatcttta gttatgacac cttttctatc
     1021 tttaattaat tcagtaaagt caattgaggt tgacataaga agacttacat tttataatat
     1081 atagccattt caatcataag tataaatagt caatgcaata atttttctta gaattattaa
     1141 aaaaatacaa attaatttat taataataaa atttgctctc ttcattcttg actatccaga
     1201 attgttcatt attttatttt cctcatgata tacttattag tagttgataa ttttctggca
     1261 gtatgtcatt ttaaagaaaa ttattaatga aataatataa ttagctaact tgataactta
     1321 aacatgaata caactataat gtaattaata attaattttt taattaatta aattttcctg
     1381 atttatatta tatttctatt atccagtatc taaaaaaaca tgcaaaaaaa taatataatg
     1441 attctcatta ttaaatatat taattggata atatttgata tttttaaaat ttcaaataag
     1501 aaatgaaatt tacggaaact attcaaacgt gtattattca tccaatttgg aagagatgaa
     1561 atagtagtaa taaatcaata taatttcatg atttgggcaa atattagagg aactaataat
     1621 aacaataata tttgttacga gataattaac atactatttt tgtcatataa ttattattat
     1681 taattattat tattattatt taccattcta attatttttt cgttatgaac aaaatcattg
     1741 ttctaaataa aggaaacaca ttataaagaa aaagataaaa tctatctatg tgtaaggtaa
     1801 tagattttcc tgtgtgacaa cattaaaaat atgattttat ttttttataa ttgtattttt
     1861 attggatttc tgttcttgat ccatcaggat tatattggta aacctttcct tcttgttcat
     1921 ctttagtctt cttgtgtctc ccatcacaaa atggttgatt attacttaat ccacacatac
     1981 aaatccattt gctttcattt cctactttta cctccatcgg tcctctatct tcttttctta
     2041 caagtctggt catacgcttt atatcataat aaaatatcct tatatttcat ttcatttcaa
     2101 taattctatt aattcagatt gttttactat ttaccgccgt aaataatttt ttatctatgc
     2161 tataacagca gcaaattaca aatttaaata caaatagata atagggatct gctgaaatct
     2221 cgaaaattta gatagataga tagattcctt aacataatag gattcctatg ggctacgtaa
     2281 ctaagataaa taatatctca gctcattatt tagaaaatat atttatatag ttctagttac
     2341 ttttcgacat ttttaaatat tacgatccac atttaatcat aaggttggtt attgtttaaa
     2401 acaattttta aaaaattttc tagctctaca aataaaaaaa atatagatcc tgttttacat
     2461 ggtcataaaa attatgagga caaattattt aaatcaatag ttggattttc tgatattaaa
     2521 caatttttaa tgcgctgtat aatctctaag gaacaggtac accttctttt tactggacct
     2581 cctgctactt caaaaacagt ttttttatta gaaatgttta gagaactttc taattcttat
     2641 tttatagatt ctactgcaac aagcggcata ggtatagttg attttttatt ttctaatcca
     2701 aatacaaaat ttcttctaat agatgaaatt gataaattaa caaaaaaaga tcaaactgtc
     2761 ttatacaatt taatggagac aggtattttg actgaaatga aagctgaaaa aactaaaggt
     2821 ttcagacaac aaaaaatgaa tataaaaatt tttgctactt gtaatgaatt aactaaattg
     2881 actcaaccct taaagtcgcg atttatgaaa ctcgagttac ctgaatatac atgggaagaa
     2941 tttttaacta tctcaaataa tttaataaaa tcaagataca aatatctaga cgaaagagtt
     3001 tcaacaagaa tagcagaagt ggtttggaat gatattaaca ccaaagatat tagagatgtt
     3061 ttgcaaattg ctagacttac acgtaatcta gaagatatag aggttatagc tcatacttta
     3121 atgaagtata aaccaaagta ttaacaaaac aaccaactat tatatttttt tcttatacaa
     3181 tactttgtta acttatacat cctaacatcc ttttttttac gatttgaata gatatttatc
     3241 atcaaccaat caccatcata atcatcaatc agaatgtagt ataactaatt tattagctgt
     3301 tttaaaaatt tgaaaaataa tgactgtact gggtaattta agttatttat atcaatgtaa
     3361 tattctggat tttatttaat attaacttca actacaaaga aatgacttaa tggtcttatc
     3421 aaaaaatatt taatatcact ttgactttat tattcttttt tcatgatatg ttcttatctt
     3481 gttacttaat cttggacaac catttactga attgactagg attaatatca attcttttaa
     3541 gactacatat accataaaaa aatattgcta tatgaattaa taatattaaa gactctaatt
     3601 acaacatgga tagataaaaa tcatattttg ataaaatttc cccaataatc attgtgatta
     3661 ccatagaggt ttactattag acattaaata tttattatat ttcttggcca atctgtttgc
     3721 atcaagcgct tgaattatcc aaattccaaa gtagattatt ccaaagatca tagataaatc
     3781 aagacccggc attagtgtat cattttgcga tataaattca aataaaaata caagtattcc
     3841 gattccaaat ggaacccaac ctataaacat tattagcaca ccccgtaata cctttccaat
     3901 atagatgtgt cctataccac aaaatccaaa tattccagct attatggcga ttaagaaagc
     3961 taagccattg tttttaaatg gaatggaagc aacggtcaat ttatataaac aatagagaaa
     4021 agcggttaat aaactaactt cctctagatt ctttaaggtt atatatattt gatagccatt
     4081 ctaggcaaaa aatttttttt agtaatgtgt tgttcaaaac tataaaatgt attattatta
     4141 ttatttttct ttctgatgga tcattatctt tccaaatatg ttacagtaaa taacagtaaa
     4201 agcatatgtt aatcatattt tatatatata aaattagagg cactatctgt ttatagaaga
     4261 tagaatgacg gtttatagaa gatagaatga taattggaat tattccttaa tataaaaata
     4321 atctataata gtactgaatg aaaatagaaa tagaatataa agaataagtt aatggtattg
     4381 tctatgaagt tttcattaca tattttgtat taaatggtat acaattaaat ttaatatatt
     4441 ttattagata gttgtataag aggaaatact tattttgcat ccaaaaaatc aggtattgat
     4501 tattttattt tcaataattt taataggtct cataggtatt ccatttggta atccaaattt
     4561 tatcattaac gccattatac tagaactatc attcataata ttggctattc ttattgcaaa
     4621 aggaataggt aatactaagc cactttacgt atgtattttc cttgcactat taattattat
     4681 tggtaatagt cttgttactg cacatattaa tcgaatgata actttttcaa aaccacttaa
     4741 tactatagtt ttgattattg gtggatattt actacaaggt ttacttatat atagcagtat
     4801 attgactata aaaagaagga aacttaaaaa tcgtcaatca tattaaactt cttccttctt
     4861 ctaataacac tttggaaatg gttctaaaaa aagtatacaa ttatttatct ataaatttgt
     4921 aaactggtat aaatcttgtt aatgatattt ttattatttg agattaatat tgtttgcgac
     4981 tacctctaat agtttagttg ttgagatagg tttctttatc agttcaaaat cggaggggtc
     5041 ctcgataata ttttcatatg cagttattat gattactttt atcttttcat caatttcttt
     5101 aatctttgta cctaactcgc atccatttat ttctggcatt ctataatctg ttagtaaaag
     5161 agtacattta tcgtgatttt ttttgatata ctcaagagct tcaattggat tagtgaatcc
     5221 tataacacta tagccgttca tctctaacgt gactttaaac ataaatacta aatcagactc
     5281 gtcatcaact acaacaatag aagaacgact cattaccata agtaatagca gtatttattt
     5341 attcaggtat ttaacatgat cttaatattg ataaacctat tgattggaat ctattttttt
     5401 ggataggaga ttacttaatg tttttgtttg tttgtttgtt tgcatgttag gaatagttaa
     5461 aagtagtgga atcatttttg gataataaca ctatcataag tatatgttat tttaatgact
     5521 atatagatat agatattcaa taaatatgga aaaccagagg aatgatcaat tttcattgtt
     5581 tgtagaaacg tgtgaaaaaa ttagatctac tactaaaaaa aatgaaaaaa tagatattat
     5641 ttctaaatat atagtcaatc ttgacgaaac ctcgttatct attgcggttt tatttctctc
     5701 aggaagggtg tttcctatag gctcaactct gacactcaat ataggcttta gtactatcat
     5761 ggaatctctt tctgaaattg cattgttaga cattaaagat atccagaata ttcatctaaa
     5821 acacggagat attggtgcaa tagcagaata tgcagtatct aaaaagcata ttatttctct
     5881 atttaatcaa caagaaagac tatcattaag ttatttgtat cctcaattta aaaagatagc
     5941 aagcattagt ggctctggtt ctaataaaac taaaaagaac atattaaaag gattgcttat
     6001 tgcttgttct ccacttgaat caaagtatct tataaaaatt attactggtg aaatgcgaat
     6061 tggttcagtt gaaggtcttg ttgagattgc tctatctcgt tcatttgata gagaattaaa
     6121 ttatataaga gaagctatgc ttatttcagg agatatttca caagtagcag ttttagcaaa
     6181 aaaaaatatt cttcatagtg caaaaatgaa accatttgtg ccagtaagtt ttatgttagc
     6241 agatgttatg tttagtgcgg aagagataat aaactattac agtaaacctt tgatttgcga
     6301 atataaatat gatggaataa ggttacaaat gcataagttt gataatgagg tgcggctttt
     6361 ctctcgcaat cttgtagata taacatatgc atttcccgaa ttagtgaaag ctgcaataaa
     6421 atctacaata agaactccag atacaacgtt acagaatcag gtagacttta ttttagatgg
     6481 agaactaata gcctttaaaa atgatagacc attacatttc caagagcttc agaaaagact
     6541 tagacgaaaa aatgttactg acgatattca aactgaaatt cctatatact acatagttta
     6601 tgatataatg tattttaaga ataatcaagt attaaagaaa tcacttttgg atagaaaaaa
     6661 cattttatca acaatctctt ttaaagaacc aataatcaat tcaagttata aaattttaga
     6721 ttctatagaa caagtaattg caatatttaa tgaaagtaaa gatatgggac atgaaggatt
     6781 agtagtaaag gatccactat ctcaatatca tccaggaaaa agaggtagat actggatgaa
     6841 gttaaagaag gaactagata caattgatgc agtaattgta attgcagaat atggtcatgg
     6901 aaaaagagct ggcgtattat cggattatac atttgcagta agagatgacg ataaaaaaaa
     6961 tcataatgat ttgaatatca ataatcatct tgaaaatata aggctaaaaa caatcggaaa
     7021 agcatactct ggattgactg ataaagagat aggggaaatg actgaaaggt taagagaaat
     7081 catagtagaa gacaatagta atagaatttt agtaaagcct gaaatagttt tagaagtagc
     7141 atttgataca atccaaaaaa gtgataggca taatagtgga tttgcattac gatttcctag
     7201 gataaagaat attcgtattg acaagagcct aaaggacata gatacattag aaaaagtgag
     7261 acagatttat aaaaaccaaa tatatgtaaa gcataaggaa atttgatgaa taaatttagt
     7321 atataatata gtattaattt ctaattatag tttataatat ttatttaatg aaattatttt
     7381 tttattggct atagacaaaa gtatttggtt ttattagaaa ttaaattctt tgttattgtc
     7441 aatctataat attccatgac ttcttctatg aactaatagt tcttcttcac ttgacattcc
     7501 ataaccacaa atttcacaga aaacaagatt tgagatttca actatgtctt ttggaattcc
     7561 aacagtaagg atattttcaa gttcaataat tttatggttt ttatagattt taaaagagtc
     7621 taaatattct tgtaataatg accttatttg ttgaattact tttttatctt gttcattata
     7681 tagggataat actatgttat cttcttcaat tttgatttct attttttcct taattattga
     7741 attagaaata aaataattat atatattatt tgctagatcg atatctcctg caaaatttat
     7801 tatcaaaata ttttgattcc cccactaact gataacggat ctgctattat aaactcacca
     7861 tcaataaata aaagaattca ccaaacatat taattaaatt agaatagaat attttacttc
     7921 atctatagac atatcagtag ccatttgaga tgaaatatta gttatttcag atatctctaa
     7981 aatttgaaaa ttgtaataag aatttaattt atgagcttta gatttttatc ataccaagta
     8041 attacagaat taatttatcg gtatgatatt attattatta ttattataaa taatgtaata
     8101 taaaatagag taatttcata atttttttat aataaatctt tcaaaaattt cttttacaaa
     8161 ttcaaagttt ataactaatc aaattagtct ctgatataat actgtatctt attgtattat
     8221 tttgataatc ttctcttttt ttgagttgcc tatatttttc atttgatgaa atattgaggt
     8281 ggtggacgat cttttctttc aacacagttt atctttgacc ttatgcaatg gagctaatat
     8341 tcatcgattt tatcattgtc tcaatagaat tcatatttcg ttcaaaggta tcgggttgag
     8401 ccgtaaaatc taagctatac aatagtccat tacttattgc ctcaattcca atatttcttt
     8461 gcaactctac tgctttattt gtataatcaa ccataaacgc gttaattcct gacaatttag
     8521 tcgactgtac ttttacatca acagacgagt tattcaaatt ttttagcaaa atattccttc
     8581 tatcgtctaa acttaaggaa tctttaattt tttcaatgtt aaaccaaaca aaaaccatat
     8641 ctgatagtac tttatttgat ggagaaagaa aactaacttg aaagattcga tcaggggaaa
     8701 agttttctgg cccgttattt tcataatatc tccaatcacc aggatatttt atatttattc
     8761 catataatgg attttcataa gttttgaatt cttctgaaga ttcagaatta aatatacctt
     8821 gtatatttgg agtagaaagt aggataacta ctaaactcga gaaaatacaa actaatactg
     8881 ttatcctatt atttattacc attctataat tatattttaa tcatatatta atcttatagc
     8941 gttaataatt taatactaat attatattaa agatatttac ttaaattata aataaataaa
     9001 t
//
LOCUS       DASVEV010000051         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_12066, whole genome shotgun sequence.
ACCESSION   DASVEV010000051 DASVEV010000000
VERSION     DASVEV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12066"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VF222_02725"
     CDS             complement(<1..167)
                     /locus_tag="VF222_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7141373.1"
                     /translation="MSTNNENTDSKPKSSNEVFIGKKPLMTYVTATLVQLANEPTVFI
                     KARGKSIPMAV"
     gene            303..503
                     /locus_tag="VF222_02730"
     CDS             303..503
                     /locus_tag="VF222_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141374.1"
                     /translation="MIIAYPDDPGPLRTPFPVQLLTIAEFSLRIAPQNTNSIISSQFV
                     PAVPVVVNPFIFTFAPLLTSIM"
     gene            complement(723..866)
                     /locus_tag="VF222_02735"
     CDS             complement(723..866)
                     /locus_tag="VF222_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141375.1"
                     /translation="MNSSYYSGYGDDNNNNSYYSDNYYPPKNSDNNNKKYVCENGPFK
                     ASL"
     gene            1355..1726
                     /locus_tag="VF222_02740"
     CDS             1355..1726
                     /locus_tag="VF222_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX7141376.1"
                     /translation="MIRPFLEYLTLVIDVTAGIVIAISIIRGFIMFLKILRKSALEQT
                     KEEEGIKHYVGNGLILALDLEVGSDIIKTILTPTVPELLVLAIIVAIRIVLSWSLSKD
                     MQSYRVTNKKTENDSKMLKAI"
     gene            2663..3010
                     /locus_tag="VF222_02745"
     CDS             2663..3010
                     /locus_tag="VF222_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141377.1"
                     /translation="MDYENVGRTIMNMDPAVRFVTIFDTNNQKAIYSQHKPGVTNLLS
                     KEESQESLQLALNAWKTRSKLEPKIGKGKYVLAEYEKIKRITMPLDENHLLYITTEVG
                     FDALNLIDKVRKL"
     gene            complement(3149..3352)
                     /locus_tag="VF222_02750"
     CDS             complement(3149..3352)
                     /locus_tag="VF222_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141378.1"
                     /translation="MKDGGEMATWKGYGIGRYNGRNRTDRSSVFFKTMSTGKLAFLNN
                     KIEVFEFEADKNGNTNGKIWEWK"
     gene            complement(3358..3498)
                     /locus_tag="VF222_02755"
     CDS             complement(3358..3498)
                     /locus_tag="VF222_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141379.1"
                     /translation="MEGSTIETTIAGNGIIINGIEVTGIVTYWSKPSESDGKHSMPQV
                     GV"
     gene            complement(3480..>3729)
                     /locus_tag="VF222_02760"
     CDS             complement(3480..>3729)
                     /locus_tag="VF222_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141380.1"
                     /translation="FLNGVQGEAPGGFQLPVANAVPGDKDYSPLWQTNFVKWNDNATA
                     RELKSVEEIIAAHKNGELTITETNIIVNSPAIKWKGQQ"
BASE COUNT         1243 a          567 c          563 g         1356 t
ORIGIN      
        1 tctactgcca tgggtatact ttttcctctt gcttttataa aaacagttgg ttcgttagcc
       61 agttgaacca atgtggctgt gacgtaagtc ataagtggtt tttttccaat aaatacctca
      121 ttgctagatt ttggtttact atctgtattt tcattatttg tagacattaa ttttattatt
      181 cttaaaaact agaatataat gctttttcaa tcctgcgaca gtattgttgg acattgttag
      241 ggttatgcca agctctaagg cgaagacacc gtgttcccta taatcagtga taataggttt
      301 ttgtgataat agcatatcca gacgaccctg gacctcttag gacaccattt ccggtacagc
      361 tattgacaat agctgaattt agtttaagaa ttgcacctca gaacactaac tccattatat
      421 cgtcacaatt tgtacctgca gtaccagtag tagtgaatcc tttcatattc acttttgccc
      481 cattgttaac ttcaattatg taacgttgtt cattcacatt ggtgtttagt actgtagata
      541 gatgcattga ttatagtata cttggcacct gaacctataa ttgttagact cttacaaatt
      601 gtgatttgtt cattataggt tatactttta ttgtgtctcc tggagttgct acattattgc
      661 agattctata gaaggaaaat cagttggaac agttatatct ggtttacaaa attctacaga
      721 acttacaaag aagccttgaa tggaccattc tcacagacat attttttgtt attgttgtca
      781 gaattttttg gtggataata attgtcgctg tagtagctgt tattgttatt atcatctcca
      841 taacctgaat aataagaaga attcatatac tgtgtctcat cctttaataa tcttgtgctg
      901 cagttccttg aagtataatc atattcgcag ttcaaaagat gatgctgcta agcaagaaaa
      961 atccaactaa agtagacctg tccatatttg ttaactaaca ttttaattat atttaaaatt
     1021 tgaagaatca ttcagggaca tatactatat aatttgttta gataatgatt attatattat
     1081 tcaatagcta atttctattt ataatatcaa tactgaaaaa tgttatactt tgaatctaat
     1141 tttacaaaat ataagaatga taatgtgcaa tctcatttga atttgttaga tgatatcaaa
     1201 atagataagt tgtagtaaac tataagtgaa ataattagaa tatattctct atagtatcaa
     1261 gcataactta aaaactatct acatctttat tatgtcctca ttttgatact tataatgaga
     1321 taagatagaa catccttcct tcgaccctgt agagatgatt cgaccttttt tagaatatct
     1381 tacattggta attgatgtaa ctgcaggaat agttattgca atatcaatca ttcgtggatt
     1441 tatcatgttt ttaaaaatac tacgcaaatc tgcattagaa caaacaaagg aagaggaagg
     1501 cataaagcat tacgtaggaa atggcctgat tcttgccttg gatctagaag taggtagcga
     1561 tattattaaa acaatactaa ctccaacagt accggaactt ttagtacttg ctataatagt
     1621 agcaataagg atagtactta gttggtcgtt gtcaaaagat atgcaatcat atcgggtaac
     1681 aaataaaaag acagaaaatg attctaaaat gcttaaagcc atataatgta tatatataac
     1741 ttagagtata aaaggtcaag caatcagaga atcattattc taatgacatc ttttcattat
     1801 aaacctaaat aataaatatc attagtacgg ttcaccttct aatagtaggt tatctacata
     1861 ctaaggaagt tggtcgggag tggattcgtc catgtagtag ggttttccca ctagcctgta
     1921 ttaagctttt attataagaa acttatttta gatttgtttt tgaaagtttt gagatatgtg
     1981 gattcattag gaatttgcga aggaggaata ataccgatag tatagcggta caaaaataag
     2041 agtctaatgc taaagtaata aagaaagaat atttaaattt atttactatc taaaattact
     2101 tttctaaatt tactgaaata aaataaaata tatggagtta tagattaata actatgatat
     2161 tggtaacaag aatagtttag tttggtcatt tgttgacttg ttattcagtg aagcttgata
     2221 cttgcttttt ggttgggctt gatatgatat ggtctactat tacatccaag acaaatccta
     2281 tatttcaatt aatatcctat attaaatcgg caaagttagt gttaaaagta ttgaaggtaa
     2341 tagtatttgt ctttctcttt tgtttttcat ctatacaatc ggaacatact ctctattttt
     2401 tctatattat aaaatggagg tgttaaaatc taattggaaa cttttttgta attggttgtg
     2461 caaatcatat tagcaatcaa acttagaaat actttgtaaa tttggtagta tttcaaatgt
     2521 caaataattt tgctcataat tgtaaagtat ataattaact atttaagtat cgtttgcatt
     2581 attgtacatt gaagaacaaa gtaatatatt atatactatt taacaaaatg tttataacat
     2641 tacatcattt atcactatca taatggatta tgaaaatgtt ggaagaacta tcatgaatat
     2701 ggatcctgct gtgcgtttcg taacaatatt tgatactaat aatcaaaagg ctatatattc
     2761 acaacacaaa ccaggtgtaa caaatttact atcaaaggaa gaaagtcaag aatcccttca
     2821 actcgccctt aatgcatgga aaacacgcag taaacttgaa ccaaaaattg gaaaaggaaa
     2881 atatgttctt gcagaatatg aaaagataaa gcggattact atgcctcttg atgaaaatca
     2941 tttgctttat ataactacgg aagttggttt tgacgctctt aaccttattg acaaggttag
     3001 aaaattgtaa attctaaaga aatagtaaat agaattatct aaactatttt gacgaaaaat
     3061 aattttctgt tattagtaat aaaaaataaa aaaatctggt attacaaaat ttgtatttta
     3121 cagaatgatt ttacttcttt attatacttt atttccattc ccaaattttt ccattggtat
     3181 taccgttttt atctgcttca aattcaaaaa cctcaatctt attatttaga aatgcaagtt
     3241 ttccagttga catcgtctta aaaaatactg aacttcggtc tgttcggtta cggccattat
     3301 aacgtcctat cccatatcct ttccatgtag ccatttctcc tccatctttc aatcttatta
     3361 taccccgacc tgcggcatag aatgttttcc atcactttca gatggtttac tccaataagt
     3421 tactatacct gttacttcaa ttccatttat aataatacca ttgcctgcaa tggtagtctc
     3481 tattgttgac ccttccattt tatagctggg ctatttacaa tgatatttgt ttctgtaatg
     3541 gtgagttctc cattcttgtg agcagctata atctcttcta cagactttaa ttctcttgca
     3601 gttgcattgt cattccattt cacaaaattt gtctgccata atgggctgta atctttatct
     3661 cctggtactg catttgcaac tggaagttga aagccacctg gtgcttcacc ttgaactcca
     3721 tttaaaaat
//
LOCUS       DASVEV010000052        13131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1372640, whole genome shotgun sequence.
ACCESSION   DASVEV010000052 DASVEV010000000
VERSION     DASVEV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372640"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..139
                     /locus_tag="VF222_02765"
     CDS             14..139
                     /locus_tag="VF222_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141381.1"
                     /translation="MPGIDGIIPVDVYVPGCPPRPETLIQGCMLLQEKIKRSKIK"
     gene            complement(618..875)
                     /locus_tag="VF222_02770"
     CDS             complement(618..875)
                     /locus_tag="VF222_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141382.1"
                     /translation="MSFYRRIPDIRDIQPNSINLGFPTESIANKVIQQQRELINNIGI
                     SSLTHPFNHSRMAEVDSIVKEDLDRKIRVLTRQSEDFDKIG"
     gene            1314..1703
                     /locus_tag="VF222_02775"
     CDS             1314..1703
                     /locus_tag="VF222_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141383.1"
                     /translation="MDSKLSSIVITSVLAFALIAGIPAVFENVLAQQDGSGQTGGDAG
                     MDQSQTGPDAGMDQSQSAADAGVPLTQGNDTGFAQSQPGADMGGMTPDQSSADSTSGD
                     GGSGDGGSGDGGSEDNGDKEDNGDKEE"
     gene            2249..2584
                     /locus_tag="VF222_02780"
     CDS             2249..2584
                     /locus_tag="VF222_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141384.1"
                     /translation="MSEDLKDSSLGLVVKGYEIIPNKPLLYRIALYSADIVESILNAN
                     PEIVELWLQKLQEEYSILKKRCDLDQDPSACFNLGFYEEFEKNFTNYENGGSIKISVT
                     KKKFIWIRK"
     gene            complement(2840..3385)
                     /locus_tag="VF222_02785"
     CDS             complement(2840..3385)
                     /locus_tag="VF222_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7141385.1"
                     /translation="MELELTLPVCSSRDHIQGPQKAPITLLEYGDYECPYCGHAFTIV
                     KLVQNLLGDNLRFVFRNFPLTQIHPHAQHAAEAAECAGAQNRFWEMHDILYEHQQALK
                     DEDLESYADLLHLDIPKFQSDLYNHSFADRVREDFLSGIHSGVNGTPTFYINGRRYND
                     SLDGETLTASLRSVINKDNIE"
     gene            complement(3388..3912)
                     /locus_tag="VF222_02790"
     CDS             complement(3388..3912)
                     /locus_tag="VF222_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7141386.1"
                     /translation="MSKSLAQQKEIPVLQPGNTAPNFTLRTTPDQSVSLDQFKGNPVI
                     LVFYPADFSPVCGDEVSLYNEILPEFQRFNAEVIGISVDGVWCHLAFSKHKNLRFPLL
                     SDFEPKGNVAQQYGVYRKNDGVTERALFLVDKDGVIRFSYVSPIGVNPGADGILKALE
                     SLPKSTEQEVGTTK"
     gene            4783..5049
                     /locus_tag="VF222_02795"
     CDS             4783..5049
                     /locus_tag="VF222_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141387.1"
                     /translation="MKKKDILTDDRESDYSSIKEEEHNEINPQSEKIQSVALSSSTIE
                     NIQGLQPMKPNALTMRERIKDSDKTQKEKRKMVGDGAFIAAEDR"
     gene            5697..6653
                     /locus_tag="VF222_02800"
     CDS             5697..6653
                     /locus_tag="VF222_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141388.1"
                     /translation="MMKKSLIIISTFLYISILITGLPFQINTNHIGNNSNFSLDTINQ
                     FVYAQVDGDKGDSSGDKGDSSGDKSIVDSNDGSKENTENSTPDFSNSQDDDTDGQSSF
                     SSNEPSGISNDLLDNEPPIGGGDNEPPIGGGDNTQNSKHKNDIKEKINFEFKKITKII
                     SKTENKIIVEDDRNNEKEIIIVSDPTCPTQSENVELNGIINPKSIRLLANFYPCTIEN
                     GGITMSIPESPYLKMAVIYIDNNSDNHAGTLISPTKIQSIDKNQGLFSIELDKKMKGN
                     DPITGELTTLDKINGLALYNNGNDPLEFKAGSTVALTATFHK"
     gene            6876..7220
                     /locus_tag="VF222_02805"
     CDS             6876..7220
                     /locus_tag="VF222_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141389.1"
                     /translation="MKIYYDLEATSELEIDENNNSSIESHAGYTSFILYHRLGYCCND
                     LEDTFLIDLEDPEYFDYDDVEVMKEPNVVFIIVNSIGHTKLGDDKKGFKFCPFCGEKF
                     DLQRIHGDEIKN"
     gene            7705..8148
                     /locus_tag="VF222_02810"
     CDS             7705..8148
                     /locus_tag="VF222_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141390.1"
                     /translation="MSTTTFTFLIVLGTLISVTPNYFLTSLAQQISTDLTAPTGGNLY
                     GGDKKGSLELSVQNGTVIATAEMDVPPADNQVYEGWLEDKGDASGYKLSLGKFNQDDM
                     LSVNQTMVNPYTYTVFFVTAEPVDDPDPNPAGVVAGAQLNSPFGQ"
     gene            complement(8560..8862)
                     /locus_tag="VF222_02815"
     CDS             complement(8560..8862)
                     /locus_tag="VF222_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141391.1"
                     /translation="MKIQTNLAMVAMFIVMALSFLPIYGQNIFNTTANTANTIENTAE
                     ENRKVGLDPVAPDTNKIDGIDSTELLNQVGEAAKRIAIGGANVLSNLSGEIKKGLQ"
     gene            9157..9417
                     /locus_tag="VF222_02820"
     CDS             9157..9417
                     /locus_tag="VF222_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141392.1"
                     /translation="MKYRSRTEIVSMILEAANGGATKTKIMYKAFLSYAQLREYLSVL
                     IENSLLEYLEGNQKYKTTEKGLNLLKMHNEMGELLQTKVKYN"
     gene            9976..10392
                     /locus_tag="VF222_02825"
     CDS             9976..10392
                     /locus_tag="VF222_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141393.1"
                     /translation="MFHSKVENENKQSDEIEKKVLKPKTNFENYTLQYLYNTNNTFFQ
                     LFMHTKVYDKQTNENLEKQGILLRSWNPITKKGEKYVFNKEDGEGDLNTKIIYYPTAK
                     TIIELSRKEGGEKILNLAIEIINVSPHKDIEIKYID"
     gene            complement(11462..11593)
                     /locus_tag="VF222_02830"
     CDS             complement(11462..11593)
                     /locus_tag="VF222_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141394.1"
                     /translation="MKALAIDRTDFDVINSKAYSLAELERYKEAFAVIEKVFVSNPG"
     gene            complement(12154..12477)
                     /locus_tag="VF222_02835"
     CDS             complement(12154..12477)
                     /locus_tag="VF222_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141395.1"
                     /translation="MKPAENEIPSWPLITGIAVTAFGTILLAFRASVLLSIVFIILGV
                     ILVLSWYILYTKKKSEISHNFGTDCICQICSHDKPLICIKQKCKCCTIMKENKVIGHS
                     NNPLQ"
     gene            complement(12493..12750)
                     /locus_tag="VF222_02840"
     CDS             complement(12493..12750)
                     /locus_tag="VF222_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141396.1"
                     /translation="MSDFAFFRFISNNWLTESEKKRKDEIIQITEEILESKGIECDAR
                     ISKLPEEQLKEILEEVRDKIKKKKKKNNITIESESELVVKT"
BASE COUNT         4868 a         1837 c         1895 g         4531 t
ORIGIN      
        1 ttcctataat gttttgcctg gaatagatgg tattattcct gtggacgtct atgtgccagg
       61 atgtccacct agaccagaga cacttattca gggttgtatg ttattgcaag aaaaaataaa
      121 aaggtcgaaa ataaaatgac ctgctagtat ttaaattaaa caaacttact ttcgaaatat
      181 tcgattattt attttatgaa atatatcttt tagggataag tcatttcgaa taaataataa
      241 caataaaggt tttataataa atttctgaat aatgaagtta tttttatgat tctatgatga
      301 ttaggaatga atagttggtt attgtacaat aaaatagaga ttataattga cttagatatt
      361 actaaattcc aaacatgcta ttacttttac atattctttg ataaatatta tgtatagatt
      421 agtattggaa gtgtgatgca taaacagatt ctagtaggga aaatgtcaag ttcaaatttt
      481 attatcattt gaaatagaaa ttgatatgct ttcattgaca atcctcatga gtttagaata
      541 atagtttagg ttttttttaa ttcttgaata ttatattatt gactatatca tatcatttaa
      601 aaaataaaat cgttgtatta tcctatctta tcgaaatcct cagactgtct agttaggaca
      661 cgtatttttc tatctaaatc ttcttttact attgaatcaa cctcagccat tctactgtga
      721 ttgaatggat gggtcaaaga agaaatacca atattattaa ttaattcgcg ttgttgttgt
      781 attactttat tagctataga ttctgttgga aaacccaagt ttatagagtt tggttggata
      841 tctcttatgt ctggaattct tctataaaaa ctcatcaatg tcatacttga tagcgtacta
      901 tttaaattgt aaggaaatag tcttaacctg tacatttggg tctctataga taaagtaaca
      961 tccgttttta catctctata gtaccactat cacgtcaaga aaaagctctg aattttaaac
     1021 tctgaacatc ctttgctcaa tcttgaccta atagaaaatt aatgtagcag aaatgggaaa
     1081 acaagcaata tacatgatga ccatttcgag aatacgatag tcagaatagg aattctagtt
     1141 ttagtataat atttgaataa ataaattttc ataagtaatc tagctagaaa aatatatgat
     1201 aaaattgtat ttttttctgg catcaagtta tgatgtattt ataaataaac gaaagaaatg
     1261 ttatagaaga tattttgttt aatgtataaa tactataata aataactgcc actatggatt
     1321 caaaactttc ttccatagta attacatcgg ttcttgcatt tgctttaatt gcaggaatac
     1381 cagctgtatt cgaaaatgta ttagcacaac aagacggtag tggtcaaaca ggcggagacg
     1441 caggtatgga ccaaagtcaa acaggcccag acgcaggtat ggaccaaagt caatcagctg
     1501 cagacgctgg agtaccccta actcaaggaa atgacacagg atttgcccag agtcaaccag
     1561 gagcagatat gggaggaatg accccagacc aaagtagtgc agatagcact tctggcgatg
     1621 gcggttcagg cgatggcggt tcaggcgatg gcggttcaga agataacggc gataaagaag
     1681 ataacggcga taaagaagaa taagaaatag aattatttaa aattctataa tcaatttttt
     1741 ttctcactta ttttatgtat tcttttcatt atgcgcattt ttacttttaa ttttctttat
     1801 tattgatcag ttgtctggaa actatcaatc ttccttaaat taaaaacacg ctagttaccc
     1861 aatggttaaa tcattgtatt aaccatgtta ttaatattaa agttaagaat tccttcaatg
     1921 aggtttcatt tacctaataa taattttggg tttcatctta agtaatccct cttaacttta
     1981 agcgcattaa actcaatggg gattgaaggt ggtgtccgaa gctagtatta tgaattctta
     2041 aatttgtgat acatccttga tatttgagaa tttatgttta taaataggaa tctaactatg
     2101 gtactttaga caatttaaga tcataattgg tctcagaatc acataagcat ttcttgaagg
     2161 aatcaatggt tttcttgaaa attttataac aattaatatg aataacaaaa tattaattat
     2221 ttaaataagt gttataacaa atttgtacat gagtgaagat cttaaagact cttcattagg
     2281 cttggttgta aaaggttatg agattatacc aaataaacca ctattatatc gaattgccct
     2341 atattccgcg gacattgttg aatcaattct aaatgccaac ccagaaatag tagaattgtg
     2401 gttgcaaaaa ctccaagagg aatattcaat actcaagaaa cgttgtgatt tagatcaaga
     2461 tccatctgca tgctttaatc ttggttttta tgaagaattc gaaaaaaatt ttacgaatta
     2521 tgaaaatgga ggatcaataa agatatcagt cacaaagaaa aaatttatct ggattagaaa
     2581 atagctcccc tccaatttaa tttaaaaaag ataaaaaaaa tataaaaata aattattaga
     2641 taataattaa ttattttttc aaatcttagg aagactagta agtgaaaaaa ttatattaat
     2701 agaagttcaa ccttaaataa ggaaatttag atagttttat ccgactttga aagttatagg
     2761 tagcaaaaga tcaacaagtt caatttagat atacttcaaa ttggtatata ggtatctcac
     2821 gtaagaattt tatcatatcc tactcaatat tgtccttgtt gataacggat cttagtgatg
     2881 ctgtaagagt ctccccatct aaagaatcgt tgtaacgtct accatttata tagaaagttg
     2941 gagttccatt tactccgcta tgaatcccgc tgaggaaatc ttcccggaca cgatctgcaa
     3001 aagaatgatt atataaatca gattgaaatt taggtatatc caagtgtaat aggtctgcat
     3061 aactctctaa atcctcgtcc tttaatgctt gttgatgttc atacaggata tcatgcattt
     3121 cccaaaacct attttgagca cctgcacatt cagccgcctc agcagcgtgt tgagcatggg
     3181 ggtgaatttg agtcagggga aaattacgaa atacaaagcg taaattgtca ccaagaagat
     3241 tttggactaa tttgactatt gtaaaagcat ggccacaata aggacattca taatcgccat
     3301 attcaagcaa agttataggt gctttttgag gtccttgtat atgatcccga ctactgcaga
     3361 cgggtaaagt gagttctagt tccataatta tttagtggta cctacttctt gttctgtaga
     3421 tttaggaagg gattctaaag ccttcaagat tccatccgcg cctggattga cacctattgg
     3481 agaaacatag ctaaatcgaa ttacaccatc tttgtcaact aagaataaag ctctttctgt
     3541 gacaccatca ttttttcgat aaacaccata ttgttgtgct acattacctt tgggttcaaa
     3601 atcggagagc aacggaaagc gtaggttttt gtgcttggaa aaagcaagat gacaccaaac
     3661 accatcgact gatattccaa taacctcggc attgaagcgt tggaattcag gtaaaatttc
     3721 attatataga gagacttcat ctccacagac aggggaaaag tccgcagggt aaaatacaag
     3781 aattacagga tttcccttaa attgatccaa agagacagac tgatcaggag tagtacgtaa
     3841 ggtaaagttt ggagccgtat taccaggctg tagtacgggt atttcttttt gttgtgctag
     3901 agatttagac ataatataaa atagcgatca taggttatat gcattatatc gttcaaaaat
     3961 atacttgttg aataattttt aaaagaaatt aaatattata ggttaaagac aaatatcgat
     4021 tatagatatt tgagaattaa gattatggta ttggtcaata aatcacacat cacatattat
     4081 ataattacat cttcattaaa atacgaagta aatattattt ttaaataata ttgtccttct
     4141 ctagtcatat aagtggtaga ttatcataag tattccaaga ctttatacac atttttgctg
     4201 atggcaaaca aaaagacaag cgataatagt caacttgtat tgcaattgtt gtagaaggag
     4261 cgaataacta ctgcaaagga tctatattta tcttatatac tatattatca tacagatttt
     4321 aaataattaa agactatact gtttaaatcc aattgtctgt atctttatta attattattt
     4381 attgagataa atctcgagca taattttggt ttgagtatat aatataaatt tttataaagt
     4441 cttaatgata tctggtcttt attctataat atttggtaaa tacttcaaac tatcaaatta
     4501 ttgaccaatt aagagggtat taaagttcag ttattctcct cgaagcaatc ctcatgtaaa
     4561 tagttaaaga gtagatgata atccgtttaa atgtaatatg gaaagtaatt ttaacagaat
     4621 attaatttct tgctaatttt caacttataa agagtaaaat gcagaagaaa ttgtaatctg
     4681 ccaaattttc aagaataata attttctatt agtatttatc aaatgattat atttatataa
     4741 tattgcagaa ataatattag aaagacgatg tatgtttgaa taatgaaaaa aaaagatata
     4801 ttgacagatg atcgagaatc agactactca tcaataaaag aagaagaaca taacgaaatt
     4861 aatccacaaa gtgaaaaaat ccaaagtgtt gcattatcct catctactat cgagaatata
     4921 caaggcttgc aacctatgaa accaaatgct ttgacaatga gagaaagaat taaagatagt
     4981 gataaaactc aaaaagaaaa aagaaagatg gtgggagacg gtgcatttat tgctgcagaa
     5041 gatcgataag gataggacct atactgaaat gcatagctaa attaatctag ataactgatt
     5101 tactatttca tgttacaatc taatatgatc tataattaca aatagattgc aggttagatt
     5161 atacgagaag ggttacatta aacatttatt gtttaactgg atatatatac ataaaaaccg
     5221 agagccaatt agatctactt taacttttta caaaataaat tgtaatattc aatacatgaa
     5281 aagtttctat ttggtacacc tcattgtttt atatttcatt acagccaatt gataaatcag
     5341 ataggcaatg caaacggaag atcgattata cgacacatcg ttttatatct ctaaagttga
     5401 tctactctat ttgtacacat attaacgata taataaaata atacataacc tttcataacg
     5461 aactaaatat aggttatcta ttataaggcc accccaatag ccctttgaag aatagtctta
     5521 tctaataaaa caaatataaa atacaatgta gttataatag gatgtaaaaa ttttcctcta
     5581 aaaaaacctc attgaaagaa ctatttaaat ttactatacc aaaaaaagtt aatgtgatta
     5641 tcttaaaata tgtcataacc ctttaatatc ttaataatag tcaattatta atcagaatga
     5701 tgaaaaaatc attgattatt ataagtacgt tcttgtatat tagtatttta attactggat
     5761 taccatttca aataaataca aatcatattg gaaacaattc taatttttct ctagatacta
     5821 ttaatcaatt cgtatatgca caagttgatg gcgataaagg tgatagcagt ggcgataaag
     5881 gtgatagcag tggcgataaa agtatcgttg actcaaatga tggaagcaaa gaaaataccg
     5941 aaaatagcac tccagatttc tctaatagcc aagatgatga cacagatggg caatcctcat
     6001 tttcttcaaa cgaaccatcg ggtatatcaa atgatttatt ggataacgaa ccaccaatag
     6061 gaggaggaga taacgaacca ccaataggag gaggagataa tacacaaaat tcaaagcata
     6121 aaaatgatat caaagagaaa attaattttg aatttaaaaa aattactaaa attatcagta
     6181 aaactgagaa taaaataatt gtcgaagatg atagaaataa tgaaaaagaa atcataatag
     6241 tgagtgatcc gacttgtccc actcaatcag aaaacgttga gttaaacgga ataataaatc
     6301 caaaaagtat aaggttatta gctaactttt atccatgtac tatagaaaat ggtggaataa
     6361 ctatgagtat acccgagtct ccatatctaa aaatggctgt aatatatata gacaataata
     6421 gtgacaatca tgcaggaaca ttgatcagtc caacaaaaat ccaaagtatt gataaaaatc
     6481 aaggtttatt ttcaattgaa ttagataaaa aaatgaaagg aaatgacccg attacaggag
     6541 aactaactac attagataaa atcaacggac ttgcattgta taataatggc aatgacccgc
     6601 tggaattcaa agcaggtagt actgttgcac taacagcaac tttccacaaa taatttaaag
     6661 gcttattgga atagcatttt tctattagaa agctatcgat ttaaagaatt acctattgtg
     6721 gaagtaactt tttcttttac aaatactaaa tggacctcaa aatatttaga ccaactcgat
     6781 tggaatatcc tacacattct aatatttttt attagatttt tagaatctaa atgaatttat
     6841 taacaaaaat ttttaatcat tttagaaaaa aattcatgaa aatatattat gatttggaag
     6901 cgacatctga attagaaata gatgaaaata ataattccag catagaatca cacgctggat
     6961 atacaagttt tatcttatat catagacttg gttattgttg taatgactta gaagatactt
     7021 ttttaatcga tttagaagac ccagaatatt tcgattatga tgatgtcgaa gttatgaaag
     7081 aaccgaatgt tgtttttatc attgttaata gcataggaca tacaaaatta ggcgacgaca
     7141 agaaaggttt taaattttgt ccattctgcg gtgaaaaatt tgacttacaa aggattcatg
     7201 gagatgagat taaaaactga ggtactaaag ataataagta attaagtata ataataacat
     7261 agagattgca gctccaaata cttgatgaaa acgtttagca aaaaaagatt tggtagcaat
     7321 aaattggaac agtacttgta tttatttatt tattttaata taatatctac tcatacgttg
     7381 aaaagaccaa gttaagatga agttttaatc acatgtaaat ttgtgtttca ttacattaaa
     7441 aataaatgat gtttggataa tcaaattcaa ctcggaagta tcttggattg atattcaata
     7501 tccatcagat tttggcaact gataaaatcc tcttagaaaa gatacttaag atgatgaaag
     7561 ctaggttttt tactttttat gattctaact tttaaaaaat aattggttta gaataattaa
     7621 tatcttattt attatataat taatttggta aaagaaactt attaataagt aataaattaa
     7681 ttataatcaa tgcaaaggtt gaaaatgagt acaactacat ttactttttt aatcgtactt
     7741 ggaacattga ttagcgtaac tcctaattac ttcctaactt ctctagcgca acagatatct
     7801 acagatttaa ctgcccctac aggagggaat ctatatggtg gagacaagaa agggagcctt
     7861 gagttatctg tccaaaatgg aactgttata gcgactgctg aaatggatgt acctccagca
     7921 gataatcagg tttatgaagg atggttagaa gataagggtg atgcatccgg gtataaatta
     7981 agtttaggta aatttaacca ggatgatatg cttagtgtaa accaaacaat ggtaaaccca
     8041 tatacctata ctgtattttt tgttactgcc gaacctgttg atgatcctga tcctaatcca
     8101 gctggtgtcg tagcaggagc acaactgaat agcccatttg ggcaataatt ttttttaaaa
     8161 attgatatca taaaattatt tgtaactttt attttaatct tatatgacat tattatttca
     8221 ttgagtaatt tctctacttt ttcttttcat caaggccaga tatacaattt tacattttca
     8281 gatttaaaat ttttaattac aataaaaata ttaagaatta ttagagataa actcgtttcg
     8341 ttttttaatt taaaattagg tatgaatatt gttttgaata atgatacagg agataggagg
     8401 gaaagtaaag ttttttgtta tcagaatgat atacacatat taaccattaa aaaaataaaa
     8461 ataaactata attattgttg ttatatcttt ttttacaaat ctcttttaga ttagcagagg
     8521 gctaatattg ttatctactt tcaatttttt aaattattct tattgaagac cttttttaat
     8581 ctcaccgcta agattagata aaacattagc tcctcctatt gctattcttt tagcagcttc
     8641 cccaacttgg tttagaagtt cagtggaatc tattccatct attttatttg tatcgggtgc
     8701 tacaggatct aatccaacct ttctattttc ctctgcagta ttttctattg tgtttgcagt
     8761 atttgcagta gtattaaaaa tattctgacc ataaattgga agaaaactta atgccattac
     8821 aataaacata gctaccattg ctaaatttgt ttgaattttc ataaaaaaca tagtaaaatc
     8881 taatttttat aagtatctat agaaattaaa gaaaaaaagg aggtaatatt ataatagaaa
     8941 attaacacag tatcatctag actatcaaat acggacatga gtcaaactca aactattata
     9001 aggacagatt atttattata tattatttat aattgatctg aaccgttaaa cactgatttc
     9061 aaggagggat aacaataaat ttatcctttt tctataactt ttttatctgc tattgtgaca
     9121 ctatattaaa ataaacaaat taataaagta aagaatatga aataccgaag tagaacagaa
     9181 atagtatcaa tgatactaga ggcagcgaat ggaggagcaa ccaagacaaa aattatgtac
     9241 aaagctttcc taagttatgc acaattgaga gaatatcttt ctgtgttgat tgagaactct
     9301 cttttagaat atctagaagg aaatcaaaaa tacaaaacta cagaaaaagg attaaactta
     9361 cttaaaatgc acaacgaaat gggagaattg ttacaaacta aagtaaaata caactaattt
     9421 taatcttatc actgtaatct ttttttatta ttttggataa tttatttttt tgaatcgcga
     9481 ggtatttaga ttttaaatcc gaggatacaa aatgttgtat gaatttttaa actaaaattt
     9541 taactgtatt actaaaagtt ttccggaact cttttgttaa aaaatggcga attttattat
     9601 aaacatcaat agtatttttt agaaaaagat tataaaaatt cctttagata attcaaatta
     9661 cactagaaca ctgcaaccgc tatgtcttcc ttaaactatg agcaacacaa tttaattgag
     9721 acatcccgca tccctggtat taagaatcaa agcgctaaaa atttttttat aaactaatta
     9781 acatcgttct ttttgtttgt atttctcatt ctctatagtt attagttcta taaccttcat
     9841 ctcctttgaa tattttttta aggaaaccac ttaggtgaaa tactcattta aaagcgttta
     9901 ttattctaca aaattaaata atagaaatgc taataaacta cgatgcctat atttttagga
     9961 gattaatata aaataatgtt tcattcgaaa gtagaaaatg aaaacaagca atctgatgaa
    10021 atagaaaaaa aagtactaaa accgaaaact aactttgaga attatacttt acaatacctt
    10081 tacaatacta ataatacctt tttccagctt tttatgcata caaaggtata tgataaacaa
    10141 actaatgaga atttagaaaa acaaggtatc ctattacgaa gttggaatcc aataactaaa
    10201 aagggtgaaa aatatgtttt caataaggaa gatggcgaag gagatctcaa tactaaaata
    10261 atttattacc ctacagcaaa aactatcatt gaactatccc gtaaggaagg aggtgaaaaa
    10321 attcttaatt tggcaataga aataattaat gtttccccac ataaagacat agaaataaaa
    10381 tatattgatt aattcttaaa gtttatcagt tacctaatta tgattctaaa atatagttaa
    10441 tcattttttt ctagatgata ttattgattc cttaattcct ttccattttt gaactaatta
    10501 aaattgtaaa tacatactct tgtttcgaaa tcttatcaag ttttagagta gcatctatta
    10561 aattctcaaa ttcaatgttt gaaacatatt agaacaaaaa aactgaaatt taaaatgagt
    10621 tttaagctta gtgagggagg cggaaaattg taaaatatcc tagaaataca taaagacatt
    10681 gtgaggtctt atatcgtcat ttaggcattt tcagtatatc tttaaatata gataaccccc
    10741 tcttgttgtt cttctctgtc ttgtattatt gcatactctt taaaaatcat gaagattcta
    10801 aatcctttag cactatcaca gagttaatcc aatatttctt taattttacc gctaattctc
    10861 tcactacatc ggcttattca ataccaatgc gattgcttca tacttgaaca atattataaa
    10921 cagttacacc tacaatgttg tgatttatag gaatattttt cctagtttcg acatctagcc
    10981 aatctccaat tattgataaa ttctcactca ctttaataca tttaaaatga gtcaaataag
    11041 tcaaaataat tgtcaaatca atcaaaataa ctgtcaaaat aatcaaaaaa acaagtttaa
    11101 ctactatatc atatagcctt gctataatct ttatgatcat aaaacttttg gttttggaat
    11161 gccaaataat ttgatattta tcaaatatta tatttccaga aaattatgtc aattgataac
    11221 tcaattttat tcctataaaa attgagttct taataaatgt cgattataaa aaatactgtg
    11281 gaatctataa aattttggag tattgttata cttagaataa gatggtttac atttagatat
    11341 tatttattta aattcctatt aaactgtcta ttgttttgta ttagatttct cataataata
    11401 tttggcctcc tcaattttac ctaaattgta aagtataact gccatagtat tacaaatatc
    11461 atcaaccagg attagataca aatactttct ctataacggc aaatgcttct ttatatcttt
    11521 ccaattcagc taaactatac gctttactat ttattacatc aaaatctgtt cggtcaatag
    11581 ctagtgcttt catagtatta tatagcttca ttatatcttt ttaagctttt taaggcaacc
    11641 actttgtgat ttagagtagc aacatcttca taggggatag ataggacttt atcatagtaa
    11701 tctataacgt cacatatttt tgttgactat aaaacaagga ctctattaag tatgtcacta
    11761 tgtctattga tttatcattg atgatgaaag ttgaaattaa gactttagga acaaaataac
    11821 cctattttgc aaaattgtta atcttttgta ttaatgttta tgttgaattt ggagaaacct
    11881 aaaagaaatt aagtaggcat atgtaataaa aaatatacac aaatgaacct taaagaatat
    11941 tattataaag gggaatatct tatatatagt atttcaatta gtcaaataat attttttttt
    12001 gggtctcatt ctttttatct tctttaaaca ttttttcaaa tgtgtttact aaatgtatat
    12061 ttatttcatt tagaaattct ttcaacactt taccccaaga cagctagttt gtatatttcc
    12121 aaaatacaaa tatgcaaatt ataaaatctc aaatcattgt aaagggttat ttgaatgtcc
    12181 tattacttta ttctctttca taattgtaca acatttacac ttttgtttga tacaaattaa
    12241 aggtttgtca tgactacaaa tttgacaaat acagtctgta ccaaaattat gagaaatttc
    12301 cgactttttc tttgtataca atatatacca tgataaaaca aggatcacac ctaaaataat
    12361 gaatacaatt gatagcaata cacttgctct aaaggccaat aatattgttc caaaagcagt
    12421 tactgcaata cctgttatca aaggccatga aggaatttca ttttctgctg gtttcatttt
    12481 taattgtatt tcctaagttt taacaaccaa ttcagattcc gattctattg taatattatt
    12541 ctttttcttt ttctttttaa ttttatctct aacttcttca agaatttcct ttaattgctc
    12601 ttcaggtaat ttactaattc gagcgtcaca ctctattcct ttagattcta gaatttcttc
    12661 tgttatttgg ataatttcat cttttctttt cttttcggat tctgtaagcc agttgttact
    12721 tatgaatcta aagaatgcaa aatcgctcat aagataatat gcattatctt ctagttaact
    12781 ttatcttatt tgacttttta catgacgata aataatcaat agaataagaa tacttattgt
    12841 tggaattaaa tacattaagt catgtaatta gtactactgg aattgataaa ttagtcaaga
    12901 cctattttaa aagttataat cttaattgaa tattagtctc tttcacgtcg ataatgaagc
    12961 ctggcttgca acacacacca ttcctcccta ggattttaaa aattctcgtc taaggtttta
    13021 atcatataaa taaatgaacc ttaacaaaaa aaggtaaaaa aagtatatgc gattattcgc
    13081 aatttttctc aagaatgttg taatagatca atcctacctt cttcatatta a
//
LOCUS       DASVEV010000053         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_12955, whole genome shotgun sequence.
ACCESSION   DASVEV010000053 DASVEV010000000
VERSION     DASVEV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12955"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1597)
                     /locus_tag="VF222_02845"
     CDS             complement(182..1597)
                     /locus_tag="VF222_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7141397.1"
                     /translation="MDTLHVLYIILLTFWFSTIPVACLTMTRFIRNRKIFVEKDLRRI
                     HNDPIIIFQITTRSATRITVVERGIYSIINAAIKINYSNYKISIVTEDENDKQKFPHL
                     KNCEIVMVDKKYSTNAIKKSRALQFAVENRRKTGEDTNKFWIFHMDDESYVTNQTLLS
                     LLKFIREGKGIASEGPIVYPLKFEIANPITALAESVRPFGCYDCVSQMKNPPPLHMHG
                     SNLLVRSDAEDLIGWNFGSTLAEDQLFGYKLYEKYGKKALGWHGGILLEQPPLNIKDH
                     FMQRRRWVLGSLQNINKFPFWHRFKIAFKLTSYFMGFISGIASAIVYVITFMSMLPTS
                     IQILVDQNISNPLIWAQYITSSFFPSSFPDITLKINEMASNHSIYPIDIGPISILLLF
                     PYIMWLLSYQIGLFLNLKHNKKYPLSKKIILHIQAAFLSLILGIIETFPAFYAIIEYY
                     WRNRLNAIKKIHNYDFYVINK"
     gene            1774..>2022
                     /locus_tag="VF222_02850"
     CDS             1774..>2022
                     /locus_tag="VF222_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141398.1"
                     /translation="MPSNSSGSNNNSNNNNNNSNSSGNSGGGGAGGGGGAAASAGAGV
                     AGGNNTSLKVLEAYTRDVGRGIARIDYDTMDSLAVSTGD"
BASE COUNT          684 a          291 c          323 g          724 t
ORIGIN      
        1 cttttgttat aaaattagtt gcactctgat atccaccata tgaagttatt agcagcatca
       61 ttgacacact aaaataaaat acgattagag tatttctctt tgattttata agtctatgat
      121 taacttttat aaagtcaatt atgctaatat tatattaata tagttatctt gtatattttt
      181 gttatttgtt tattacataa aaatcataat tatgaatttt ctttatagca ttcaacctat
      241 ttctccagta atattcgata atcgcataaa atgcaggaaa tgtctctata attcctaata
      301 ttaaagatag aaatgctgct tggatatgca agataatttt ttttgataaa ggatattttt
      361 tattatgctt caagtttaga aatagcccta tttgataaga taataaccac attatatagg
      421 gaaaaagcag tagaatagaa ataggaccta tatcgatagg atagatactg tgattacttg
      481 ccatttcatt tatttttaat gtaatatcag gaaaagatga tgggaaaaat gaagaagtaa
      541 tatattgtgc ccaaataagt ggattagata tattttgatc gactagtatc tgaatagatg
      601 taggtagcat tgacataaat gttataacat atactattgc agacgctatt cctgaaatga
      661 aacccataaa ataactagtc aacttgaaag caattttgaa tctatgccaa aaaggaaatt
      721 tgttgatatt ctgtaatgat cctaggaccc atcttcttct ttgcataaaa tgatctttaa
      781 tatttagagg aggctgttct aataaaatac ccccatgcca acctaatgct ttttttccat
      841 atttctcata gagtttgtaa ccaaaaagct ggtcttctgc taaagttgag ccaaaattcc
      901 atcctattaa atcctcggca tctgatcgaa caagcaaatt gcttccatgc atatgaagcg
      961 gaggaggatt cttcatttga gatacgcaat cataacatcc aaatggtcta actgattcgg
     1021 ctaatgcggt aattggattt gctatttcaa atttcaatgg atatacaata ggtccctctg
     1081 atgcaattcc tttaccttct ctaataaatt ttaataatga taataaagtt tgattggtaa
     1141 catagctttc gtcatccatg tggaatatcc aaaacttgtt agtatcctct ccggttttcc
     1201 ttctgttttc tactgcaaat tgtaatgctc tggatttttt aatagcattt gtactatatt
     1261 ttttatctac cataactatt tcacaattct ttaaatgagg gaatttttgt ttatcgtttt
     1321 cgtcctcagt tacaattgaa attttataat tagaataatt aatctttatt gctgcattta
     1381 taatcgaata aatacctctt tctacaaccg ttattctagt tgcagatcta gtagtaattt
     1441 gaaaaataat aatgggatca ttatgaattc tacgcagatc tttttcaaca aatattttcc
     1501 tgtttctaat gaatcgtgtc atggtaaggc atgcaacagg aatagtagag aaccaaaatg
     1561 ttaataatat aatatataaa acgtgtaatg tatccactaa aaatgcttta acttattatt
     1621 aaataaaact ttaactaaat tatgtttgca aaagtattaa tcacatcgtt gtaattatat
     1681 ttgattactt tttcctattt tgtaatattc acctaacagt agaaacaaag tatatgtagt
     1741 caattcacct aaggtataca agtgaacagt agtttgccta gtaatagtag tggcagtaat
     1801 aataatagta ataataataa taataatagt aatagtagtg gcaacagtgg tggtggtggt
     1861 gctggtggtg gtggtggtgc agctgctagt gctggcgcag gagtagcggg aggaaacaat
     1921 acatcactaa aagtgctgga agcttatact cgtgatgttg gtagaggcat agcacgaatt
     1981 gattacgata ccatggattc tttagcagtt tctactggtg at
//
LOCUS       DASVEV010000054        11787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1178663, whole genome shotgun sequence.
ACCESSION   DASVEV010000054 DASVEV010000000
VERSION     DASVEV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178663"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..625)
                     /locus_tag="VF222_02855"
     CDS             complement(494..625)
                     /locus_tag="VF222_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141399.1"
                     /translation="MDGTNSKSRNSDSKSWYYDSIGIYDAIKNIYTVKGDLIDNSAY"
     gene            862..1698
                     /locus_tag="VF222_02860"
     CDS             862..1698
                     /locus_tag="VF222_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141400.1"
                     /translation="MVKPCIFIIFIFFILVGSQQFSVFGHSFESDDSVYLLTLLEKTQ
                     TELELAMKNYPSNVTLSMDHSDDAARLVYDTFYVDEDVANDGEFAKRYNQEISKSNIT
                     SNALVVVTLVDEVLRSYADSIDLTFDLTNMSNLLYIIPTNSNNNNDASNQSIDKLESS
                     FFATAFNNSENSNVNVNNSDSKNPLFLTNHTIMNYAKHETAKTLAMKINTLFQLYLKS
                     FIGLDKAKLNDVRKLEDDFYKLYSMIDKNSSPAEIMKKVHIDIQPTMQKVYNLKTKMS
                     QT"
     gene            complement(1984..2136)
                     /locus_tag="VF222_02865"
     CDS             complement(1984..2136)
                     /locus_tag="VF222_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141401.1"
                     /translation="MQTSITNNANKAYNINKNGSRFLPVFDSFTLLAITLAVIIGIVS
                     YYFNNK"
     gene            2265..2411
                     /locus_tag="VF222_02870"
     CDS             2265..2411
                     /locus_tag="VF222_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141402.1"
                     /translation="MPIIKPLPAGEHEIRTEVFQDIPSIIMNDVPLHRVITYNLTVDS
                     TDKK"
     gene            2661..2822
                     /locus_tag="VF222_02875"
     CDS             2661..2822
                     /locus_tag="VF222_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141403.1"
                     /translation="MTTSNKKDYHFVIVGTLEDESKEVAFEFLKALMKKEGIKEVSEL
                     HIFGEKDKK"
     gene            3089..4396
                     /locus_tag="VF222_02880"
     CDS             3089..4396
                     /locus_tag="VF222_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX7141404.1"
                     /translation="MDIVEQKGGINDDDISIFQINLGPITLKWVAKHFGYIHNRQFQD
                     KMIKGPFKKWVHTHSFIPQDPNQCIIEDKIDYMPRFGKIGSKIIQKRIQHYLNQLFLY
                     RSRILVNDTILEKMTQEKGKNILITGSHGLIGSSLIPLLTNIGRHKITRLVRKVNNNN
                     MGLSHTTKKEDKKSIFWYPEYDKLDHHELEGFDIIIHLAGENIFGRWTDIKKQRIFDS
                     RVGSTKLLSKSLTKLSNPPSLLICASAIGYYGDRPNEYLTEDSIPGKGFLSEVCQKWE
                     NATKDASEIGIRVVNTRFGVVLTPKGGILQKLLSPFKLGLGITIGNKDQYISWVSIED
                     VIKSIFYSITNTSIKGPVNVVSPKPVTNLEFSDTLKKIYNPKLSVSINQNIPKLIFGE
                     MSKEVLSTNTNVIPKKLVSTGYKFFNLELEDSLRFLLGKIITK"
     gene            4866..5423
                     /locus_tag="VF222_02885"
     CDS             4866..5423
                     /locus_tag="VF222_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141405.1"
                     /translation="MLKLDHSSKYLILYSTFAGFISAWAISGLLVIVDIISQTPVGTF
                     FAVIGTSLGFYDPAVSQYIGFALHIATGTVAGNIFGQVALFWNKLFPSNFKRGITYGI
                     IAGVSLWIVLFLPLATFIIQPKLDAFAVSAPNQYLFGIADHFQGLYPLVVIGSLGFHI
                     IYGILFGFIVWRMIALKTVVQTNKE"
     gene            5717..7087
                     /locus_tag="VF222_02890"
     CDS             5717..7087
                     /locus_tag="VF222_02890"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7141406.1"
                     /translation="MYNSKNTKQNLRILVTGATGFIGSRIVKRLLADGHYKVRCMTRN
                     PENIYELFSLSGDIVEIVKADASNYLELVEALKEVDIAFYLIHSMEGSTKKWQEFSTR
                     DRIAAENFAKAASQTNTKRIIYLSGLIDMKHKELSEHMQSRKEVGDILRTSKAEVTIF
                     RAAVILGQGGGSFQMLQYLVERLPVMVCPKWVLTKSQPISVDDVVTYLIKSLELEDTA
                     NKDFDIGGPEILSYLDMMKRYSAILNKSLKIVIIPFLTPRLSSYWVDLITPVKASLAR
                     PLIDSLKHEAIVHDNEIQKLIPIKLMNFEESIKTAMKEQVRKAKIIKKQKTSDSLNRK
                     ILIFSLISLGIIGATYYFLDNKNDLFTPLWIILAIVWYFGILFALFFVKDGARLGSLV
                     AGIIGWITLIFWGFNNVYLIYDNSINLDYPNVLVAIRNFMGAIVAGLVIAASHNLFHK
                     IRVLGK"
     gene            complement(7276..8160)
                     /locus_tag="VF222_02895"
     CDS             complement(7276..8160)
                     /locus_tag="VF222_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX7141407.1"
                     /translation="MVYIRIKKIKNIDYAYLVESKWNKEKHTSQQIIKQYLGPVSNIN
                     INKIPEDYRNESTILKFLSSHNLDYSQSIKYNESLQEQLYKKLKNYAINDLIKLYNEY
                     TKTNYLLLDFYEKLLIPVLYKIGNQWENGEIDIATEHVCSNAVNNLIDLINQKNTKQV
                     FKSYEYTKAIVLCTPEGELHSIGCKIIESLLLEKGYEVYNITSSLPTNSIKSYLNNIK
                     FGLLLISVTLEENLDSVIRFVKEIRNSCNIPIIVGGNAIKFASKSQIQLLEKYDKVYF
                     DLKKLGNLITNIKILIDN"
     gene            complement(8620..8997)
                     /locus_tag="VF222_02900"
     CDS             complement(8620..8997)
                     /locus_tag="VF222_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141408.1"
                     /translation="MPHIVLTNVSDFKSFYNNFKKEVLIIKNAKDQTNTIIRFEEIFL
                     NQLENIILIKTIVIENNNQPQTYYIMTMKKNNQITIRLDPLTDPQNKTDAVKTSLANI
                     AKQYKKIDPAISIGKTNLGQYLL"
     gene            complement(9626..10213)
                     /locus_tag="VF222_02905"
     CDS             complement(9626..10213)
                     /locus_tag="VF222_02905"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HEX7141409.1"
                     /translation="MSKIDNSKSSINLSPFTQLISDFKGKNIADEQIQADYNNTLNYP
                     NNYFVAPDRWRECFYTTSRENMLLLSKGLENIVRIKPPFNKFVLISLFQRTSNFNSVA
                     EQYASISPISKKTYLIGGKGLAQGINSSNVEIIDFTDSGLENNWVVIAIANDEGCALI
                     AEEFKPGRYRGFFTTNRDLTNRCLNIIRELLNIVV"
     gene            10753..11697
                     /locus_tag="VF222_02910"
     CDS             10753..11697
                     /locus_tag="VF222_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141410.1"
                     /translation="MSERKNDNVILRPTSSNKSENRLRDFGYGLHYKNISAKILRNIQ
                     YPFFTMIKDDYFPTILNLTNNDQRERTDNIVVVIHDPRDISNRIIELIRKWKIITIRR
                     AVQHYIQQKYNLNSVFLYHPFYAYPTESSILNRKGAVSISRIGFGKNIDIILKANKIL
                     ESFGINGIRLYGYPTPMYIYLHLNGKNGAVEFKKHYYGKFDKSFLSVSNIIAKKKFVV
                     DLSLVKNDGGGTQYTYLEAIHNDCALILHRRWIQDLNNDPKYCDFKEGYNCFAIDNEK
                     ELAELIKVDPDTTKIISNAKRLMHRHTNIDWSKVIDNN"
BASE COUNT         4376 a         1559 c         1667 g         4185 t
ORIGIN      
        1 catttgctat tctgaataat gctatgttga gttgaagtta aagtgataag gataaacgaa
       61 gcttgattct atgatttttt gtaaatagta accagaaatt gaagaagtta aaaatattaa
      121 cattgtgtta atgttaaaga acatttaata ttactatatt caactttaga aaaagaattt
      181 gaatggttag atatttgtag taagaataat aatattagct aaaacaagta ttgttgttac
      241 atttggtgtt taattattta agtttagaat gtagttataa aaagataatt tttaatgttt
      301 attatggtaa aagaaataga ggcaaaaatg cctcgcaata aatgaacatg acaaaataga
      361 tattgaccca ttgttgaaac ttttaactaa actagttgaa ttgataagga gttaattgta
      421 taaatagtaa tttaaattat attgttccta tcttatagaa tattactcct tgagaaacta
      481 ttaaaaataa cctttaatat gcagaattat ctattaaatc accctttaca gtataaatat
      541 tttttattgc atcataaatt ccaatagaat cataatacca agatttggaa tcggaatttc
      601 tactcttact atttgttcca tccaaacaat tttcctttca ttaatatttg ttgtttcatt
      661 agtgtactat attccaatgt tgatattcag agatcattaa aatcaataaa aataaaattg
      721 aatcgtattt gcattaaaaa ttaagttatt attgtcaatt actttgcaca ttagaataac
      781 taattaaata attaaaaatc ttttacacat ataattttat acaaatttac ttgactgata
      841 tcaaaatcaa gatgatataa tttggttaaa ccatgtatat ttataatttt catatttttt
      901 atattagtag gttctcaaca gtttagtgtt tttggacaca gctttgaatc tgacgactct
      961 gtatatcttt taactttact agagaaaaca caaacagaat tagaattagc gatgaaaaat
     1021 tatccatcca atgttacttt atctatggac cattctgatg atgctgctag actggtttat
     1081 gacacatttt atgtagatga agatgttgct aatgatggag agtttgctaa aagatataat
     1141 caggaaatat caaaatcaaa tatcacttct aatgctttag tagttgttac acttgtagat
     1201 gaagttttaa gaagctatgc cgactctata gatctaacct ttgatcttac aaatatgtct
     1261 aatctcttat acattatacc aacaaattct aataataata atgacgctag taatcaatcc
     1321 attgacaaat tagaaagttc attttttgct actgctttta acaattcaga aaactctaat
     1381 gttaacgtta ataacagtga tagtaaaaat ccgctcttct taacaaatca taccatcatg
     1441 aattatgcta aacatgaaac cgcaaaaact cttgctatga aaatcaatac tctatttcag
     1501 ctgtatttga aatcatttat tggattggat aaggctaaac taaatgatgt aagaaaattg
     1561 gaggatgatt tctataaact ttattctatg atagacaaga attcatctcc agctgaaata
     1621 atgaaaaaag tacacataga catacaaccc actatgcaaa aagtatataa tttgaaaact
     1681 aagatgtcac aaacataatt atgacagtag tatctgtttt taaaataaaa taaaatagat
     1741 tacattaaaa ataaactcca caaactttat tgctttttat ttattgtttg ttgtattgta
     1801 gcacctactc caatataatg accaaatagc catagacctt catgtccaga gcgatgtact
     1861 aattgagtag tcgttttaat ctaatcttct actcaattag tacacatcat cataataaat
     1921 tagtatgaga tttgtttata taattaaata atcttcatca agcaatactt tttaattagt
     1981 gtattatttg ttattaaaat aataactaac aattccaatg attacagcta aagttattgc
     2041 aagcaatgta aaagagtcaa atacaggtaa aaatctagaa ccatttttat ttatattata
     2101 ggctttatta gcattgttag taatactagt ttgcattatg gttgacaatc agcagcagat
     2161 acaatattcc atattccata atactcaagt aaaaaaatct gttgctcata atgccatcct
     2221 tatgatataa aattggatct cataaagcaa aaataaatgc tataatgcct ataataaaac
     2281 cattacctgc tggtgaacat gaaataagga cagaagtatt ccaagacatt ccatccataa
     2341 ttatgaatga tgttccatta catcgtgtga taacttataa cttaactgtt gattcaacgg
     2401 ataagaaata atattacacg cattctagag ccttcatagc gctcacgcac accaataatc
     2461 aaataatata taataataaa tttcaacctt tgtcactaga atatgattga cataatcaag
     2521 aagagtatag ttgaggaaat aataacatca tcatcatcag aaagaatcag taaaagcgta
     2581 aagagttaac tcatttaatt ttcaggcgtc gaaaaagtta caataatctt aaatacacat
     2641 agcaaaatta tcagtttaaa atgaccacct ctaataagaa agattatcat tttgttattg
     2701 ttggaacatt agaagatgaa tccaaagagg ttgcgtttga atttcttaaa gcattaatga
     2761 aaaaagaagg aattaaggag gtaagtgaac ttcacatatt cggtgaaaaa gataaaaaat
     2821 agaatcgaga tgtcaaaagc aagagacacc atatttaaat ataataaaaa gtcattaaaa
     2881 atgagtagat agtgcctagt ttatagtaat tagggtaata atttaataaa ttatcattca
     2941 gttttattta tagtgaataa aaatagttaa taaaaattgt tcaaatatat ttgtaaggag
     3001 atcgttaatt ccctgtaaca taaataatct ttttgattat catacacgaa acggagcttt
     3061 agaaagactt gttcctcctt ggagtgggtt ggatatagta gaacaaaaag gaggaattaa
     3121 tgatgatgat atttcgattt ttcagattaa cttaggtcct ataactttga aatgggttgc
     3181 taaacatttt ggttacattc ataatcgaca atttcaagat aaaatgataa aaggtccgtt
     3241 caaaaaatgg gtccatactc attcttttat acctcaagac ccaaatcaat gcatcataga
     3301 agataaaata gattacatgc ctagatttgg taaaatagga tctaaaatta tacaaaagag
     3361 aattcaacat tatttgaatc aattatttct ttatcgttca cgtattttag ttaatgatac
     3421 tattctcgaa aaaatgacac aggaaaaggg aaaaaatatt cttattacgg gttctcatgg
     3481 attgataggg tcttccctta ttccattatt aactaatata ggaagacaca agattactcg
     3541 tcttgtaaga aaggtaaata ataacaacat gggtttaagc catacaacaa aaaaagagga
     3601 taaaaaaagt attttttggt atcctgaata tgataaatta gatcatcatg aacttgaggg
     3661 atttgatatt atcattcatc ttgcaggaga aaacatattt ggaagatgga cagacataaa
     3721 aaaacagaga atatttgata gccgtgttgg aagtactaaa cttttatcga aatctttaac
     3781 aaagctatca aatccccctt cgctattaat atgtgcgtca gcaattggat actatggaga
     3841 taggcctaat gagtatttaa cagaggatag cattccggga aaaggatttc tctctgaagt
     3901 atgtcaaaaa tgggaaaatg ctactaaaga tgcatcagag ataggaataa gagtagtcaa
     3961 cacacgtttt ggtgtggtat taactccaaa aggaggtatt ttacaaaaat tattaagtcc
     4021 tttcaaatta ggtttgggaa taacaattgg aaataaagat caatatatca gctgggtctc
     4081 aatagaagat gttataaaaa gtatttttta ttcaataaca aatacttcaa ttaaaggtcc
     4141 tgttaacgtt gtatctccaa aacctgttac aaatttagaa ttttcagata cactcaaaaa
     4201 aatatataat ccaaaactat cagtgtcaat aaatcaaaat atacccaaac taatatttgg
     4261 tgaaatgtca aaggaagtac tttctacaaa tactaatgtc attcccaaaa aattagtttc
     4321 aacaggctac aagtttttta atttagaatt agaggattca cttagatttt tacttggcaa
     4381 aataataact aaataatatc aaaataaaga tttgttattt tataacctaa ggaacatacc
     4441 ttctattagt gaactatttt ttaagtgtat aatatttttc taatgctgtt caaaggaatg
     4501 aagtgaactc aaataaatcc tgcaaatgtc attcaagccg acatgcagta attctttcgt
     4561 ttgtatgcaa atttataaga ttcagatatg aatcctcttt atcaatactt atttgtgatt
     4621 tattataaat aaatcagtat cctaaataat tttaattcta ttagattatg tccagaacaa
     4681 tttacaaata aaatcctctt catgatgtat tatttcaggg aatttaacag atccacattg
     4741 tttttattgt tatcactcgt ttaatcatat tagttggcac tatgttatat aattatgtct
     4801 attttttgta gggaagttta tgttataata taattgtttt atatgagaaa gtttatgtta
     4861 taataatgct caaactcgat catagttcaa agtatttaat attgtattct acctttgcag
     4921 gatttatatc tgcttgggca atatcaggat tattagttat tgttgatatt atatcacaaa
     4981 cacctgtagg tactttcttt gcagtaatag gaacttccct tggtttctat gatcctgctg
     5041 tatcacaata tattggattt gcattacata ttgcaacagg tactgtagca ggtaacattt
     5101 ttggtcaagt agcattattt tggaacaaat tgtttccctc taatttcaag cgaggaataa
     5161 cttatggaat aatagcgggt gtttctctat ggatagtctt attcctacct ctagccacat
     5221 tcataattca acccaaacta gatgcttttg ctgtatctgc tccaaaccaa tatctatttg
     5281 gaatagcaga tcactttcaa ggactttatc cacttgtcgt tataggatct ttaggatttc
     5341 acataatata tggtatttta tttggattta tagtatggag gatgatcgcg ttaaaaaccg
     5401 ttgtacaaac caataaagaa taaatacaat aactcttaaa gaggaagtct aattatagat
     5461 atgaatattc ttacaataaa cattaggaaa acagagatga aaattcgttt taatttatgt
     5521 ctactactat aaaaataaat gtcaaaaata aaatatttaa tcaatattct tagaatgaaa
     5581 gtaaatccat atgttatagt tattttagtg ctaattagaa ggataaatta gaatgtcaaa
     5641 aaaatgacaa ccttttatat acatcgatat caaatggaat tctatttaaa aaataaatca
     5701 tacgtaggaa ttatacatgt acaatagcaa aaatacaaaa caaaatttaa ggatattagt
     5761 aactggtgca acaggattta ttggtagtag gattgtcaag agactattag ctgatggaca
     5821 ttataaagtt agatgtatga caagaaatcc tgaaaatatt tatgaacttt ttagtttaag
     5881 tggagatatt gttgaaatag taaaagctga tgcttctaat tatttagaat tagtcgaagc
     5941 tctaaaagaa gtggatatag ctttttatct catacactct atggaaggct caacaaaaaa
     6001 atggcaagag ttctcaacaa gagaccgaat tgcagcggaa aattttgcta aggcagcctc
     6061 acaaacaaat acaaaaagaa taatttacct tagcggtctt attgatatga aacataaaga
     6121 gttatctgaa catatgcaga gtcgaaagga ggtaggagat attttaagga cttctaaggc
     6181 ggaagttaca atatttcgtg ctgctgttat tctaggtcaa ggaggtggtt cttttcaaat
     6241 gttacagtac ttggtagaga gacttcctgt aatggtttgt ccaaaatggg tattaactaa
     6301 atcacagcct atatcagtag atgatgtggt aacttattta ataaagtcat tagaacttga
     6361 ggatacagct aataaggatt ttgatatagg aggccctgaa attcttagct atctagacat
     6421 gatgaaaaga tattcagcta ttctaaacaa atcattaaaa attgttatta tcccattttt
     6481 aacacctaga ctctcttctt attgggtaga tttgattaca ccagtaaagg catctcttgc
     6541 tagaccttta attgatagtt taaaacatga agcaatagtc catgataatg aaatccaaaa
     6601 gttaattcca attaaactaa tgaactttga agaatctatc aaaactgcta tgaaggaaca
     6661 agtaagaaaa gccaagataa taaaaaagca aaagacgtct gattctctta atagaaaaat
     6721 attaattttt tcattaattt cattaggcat aattggagca acgtattatt ttttagataa
     6781 taaaaatgat ttattcactc ctctttggat aatattagcc attgtatggt attttggaat
     6841 tctatttgca ttattttttg tcaaggatgg tgcacgatta ggttccttgg tggccggtat
     6901 tataggatgg ataacactaa ttttttgggg attcaataac gtatatttga tatatgataa
     6961 ttcgattaat ttagactatc caaatgtttt agttgctata cgtaatttta tgggagctat
     7021 tgtagcggga ttggttatag ctgcgtcaca taacctcttt cataaaatta gagtactagg
     7081 aaaataaatt aattaattaa ttaattaatt ttgtttatta gaagagagac tgataatatt
     7141 aatctgagtt aatttcaaaa taaataataa ccaaaaataa ctataatgaa taattaatat
     7201 atctgtaaat aaataagaaa tatagaatct tctgtttaca tgttgtatat accatttact
     7261 tgcacattct taattctaat tatctattag tatctttata ttagtaatta aattaccaag
     7321 ttttttaaga tcaaaataaa ctttatcata tttttctaat aactgaattt gagactttga
     7381 tgcaaatttt attgcattgc cacccacaat tataggaata ttacaagaat tacgtatttc
     7441 ttttacgaat ctaatcactg aatctaaatt ctcttctaaa gttactgaaa taagtaagag
     7501 accgaacttt atattattca aataagattt aatagaattg gttggaagag atgaggtaat
     7561 attataaacc tcatagcctt tttcaagcaa taacgattca attattttac aacctatact
     7621 atgaagttct ccctctgggg tacataatac tattgcttta gtatattcat aacttttaaa
     7681 aacttgtttt gtattttttt gattaattaa atcaattaaa ttatttactg cattactgca
     7741 tacatgttct gttgcaatat caatttctcc attttcccat tgattaccta tcttatataa
     7801 tacaggtatc aataatttct cataaaaatc taataataaa taatttgttt ttgtatattc
     7861 attgtataat tttatcaagt cattaattgc ataatttttc aattttttat acaactgctc
     7921 ctgtaatgat tcattgtatt taatagattg tgaataatca agattatgag aagataaaaa
     7981 ttttaaaatg gttgattcat ttctataatc ttctgggatt ttattgatat ttatattcga
     8041 tactggacct aaatattgtt ttataatttg ttgagaagtg tgtttttctt tgttccattt
     8101 actttcaaca agataagcat aatcaatatt ttttattttt tttattctaa tatataccat
     8161 cctaataata taaacatcac caacttctct ttataatagg tgcatcatta taatacctag
     8221 tgccaaatat gacgttcgtg atcattgaaa tattattgaa atagatgtat attgaaatag
     8281 atatataatt agattaaaat aataattcgt atttctttat taattttctt acatgaagca
     8341 tattcttata cgttatctca agaattatgt ttagataaat ctatcaagga tctataactt
     8401 tataaaatga atgatattca aagtttctaa tgttccattg tttgatttgg gtcttgtgaa
     8461 ttgtacttaa tattggaatt catcccttcc ttttcatcca tttgttacca tatttagagc
     8521 tttttagagt aatttatatt attatattaa attataaaga attaaaagta ttagaaattc
     8581 gaattttctt tgatagcgaa aattctatct tctttttaac tatagtagat attgtcctaa
     8641 atttgtttta cctatggaga tggctgggtc aattttttta tattgtttag caatatttgc
     8701 cagtgatgtt tttacagcat cagttttatt ttgtggatca gttaatggat caagtcttat
     8761 agttatttga ttattctttt tcattgtcat aatataatat gtctgaggtt gattattatt
     8821 ttctatgact atagttttaa ttaatatgat attctccaat tgatttaaaa agatctcctc
     8881 aaatctgatt atggtgtttg tttgatcttt cgcattctta ataatcaata cctccttttt
     8941 gaaattatta taaaaactct tgaaatctga aacattagtt aatactatgt gaggcatttt
     9001 ttcaacaccc aattaaaaat gacacagttt aaagtaacat aataatacac tctgagcaac
     9061 tgtattcgtt ccaatttcat agcttatagt tgaatcccct acgaaaaata atccattaat
     9121 atcatctatc atattttata ctgatactta cgtttaagta ttatatggat atataatctg
     9181 tagagaaaga ggtagatact aacaaatgta tactataata atagtaggaa agtataagaa
     9241 taattacacc tacggaagaa ggtaacaacc ctatgcaaga aaaaaatgat ataactaata
     9301 ataagatcat ctatgaaggg aaacaataag acagaaaagg aaaagactga aatctttcat
     9361 ggtattgaag attaaaatat gcaattttaa attttgtcta taaagcaaag tatagaattg
     9421 atgcctgttt ggattcaaat ggaccatcag tatggttgat gttgagtcaa ttaaaggatt
     9481 aaagcaaaaa atagaggagt aaaaattaga tatgttactg agatagctac tgctaattta
     9541 caggaagcaa aaccattaaa atagcttatt ctaatacgaa gtaaaagagg taaattccaa
     9601 tactaataat atatcatttt caaatttaaa ctacaatatt taataactct cttataatat
     9661 tcaaacatct atttgtaaga tctctatttg ttgtaaagaa tcctctatat ctgcctggtt
     9721 taaactcttc tgcaattaaa gcacatcctt catcattggc aatagcgata acaacccaat
     9781 tattttctaa gcctgaatca gtaaaatcta taatctcaac atttgacgaa tttattcctt
     9841 gagccaatcc ctttcctcct attaaatatg ttttttttga tattggagaa atagaagcat
     9901 attgttctgc tacagaatta aagttacttg ttctttgaaa tagagatatt aagacgaatt
     9961 tattaaatgg cggttttata cgtacaatat tttctagtcc tttacttagt aaaagcatgt
    10021 tttctcttga tgtcgtataa aagcattcac gccatctatc aggtgctaca aagtaattat
    10081 taggataatt tagagtatta ttgtaatcag cctggatttg ttcatcagct atatttttac
    10141 ctttaaaatc ggatattagt tgtgtaaagg gactaagatt tattgaactt ttcgagttat
    10201 caattttaga catagaaaaa atatagtaat atctctagca tattaggata tcttaaaatt
    10261 ctaactttat gttaaatgct aaagaatttg tagaagagcc tgttaatttg agatattcac
    10321 ctcctttaaa tcaggaattt atttctatta ttatattgtt gtaaatggat acaaatagtt
    10381 ctatcgaatt atacacatga tctagattac acgttctcct tttcttctaa tgtagcaaga
    10441 taataaacaa ctaaacattc tgtttattat tgaaatactg cattattacc ctttctatca
    10501 gactcttctt tataggagaa tgcaatcgaa gtctattgat ttaaatctac ccaaaatgat
    10561 gatttttaaa aattatttgc atatgatgaa gcgttttttt ctgatatttt gaatataaag
    10621 aaattaaagt tgttaatact actgtcacta acttattcta aattgaattc taatacacag
    10681 ttcatatact tacataaatt cttttttgga ggcgtaacca catttactgc gcatttaatt
    10741 tatacaattg gaatgtcaga aagaaaaaat gataacgtca ttctacgtcc tacaagtagt
    10801 aacaagtcag aaaataggtt acgagatttc ggttatggat tacactataa gaatatatct
    10861 gctaagatac tccgcaatat acaatatcct ttttttacta tgatcaaaga tgattatttt
    10921 cccacaatat tgaacctaac taataatgat caaagagaac gaacagataa tattgtagta
    10981 gtaattcatg atcctagaga tatttcaaat agaattattg aactgataag gaaatggaaa
    11041 atcattacta tcagaagggc agttcaacac tacatacagc aaaagtataa cttgaattca
    11101 gttttccttt atcacccatt ctatgcatat ccaactgaat cttctatact caatagaaaa
    11161 ggagcagtct caatttctag aataggattt gggaaaaata ttgatataat actcaaagca
    11221 aataaaattt tagaatcttt tggaattaac ggtatccgtc tatatggtta tcctacacca
    11281 atgtatattt atctccattt aaatggaaag aatggtgctg ttgaatttaa gaaacactat
    11341 tatgggaaat ttgacaaatc gtttttatca gtttctaata taatcgctaa aaagaaattt
    11401 gtagttgacc tttcgctagt taaaaatgat ggtggaggaa ctcagtatac atatttagaa
    11461 gcaatacata atgattgtgc cttaatttta catagaagat ggatacaaga tttgaataat
    11521 gatccaaaat actgtgattt taaggagggg tataattgtt ttgcaattga caatgagaag
    11581 gaattggcag agttgataaa agtcgatcct gataccacca aaataataag caatgctaag
    11641 agattaatgc acagacatac taatattgat tggtctaaag taatagacaa taattgattt
    11701 ttaaaggcga attactttaa ggattaaaga ctatttatgc tatacatgat tttactacta
    11761 atataattat taaaactatt tcttcca
//
LOCUS       DASVEV010000055         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1373345, whole genome shotgun sequence.
ACCESSION   DASVEV010000055 DASVEV010000000
VERSION     DASVEV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373345"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VF222_02915"
     CDS             complement(<1..1088)
                     /locus_tag="VF222_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141411.1"
                     /translation="MTHYGFQQAQLINPAYAFQQIQDKNIDRQLTSTNPSVKEDILIH
                     TTVPILTGKSTNIEKNISTIKNSFEPNNFVIKEFPVPSGSRPHDVAPAADGTIWYTAQ
                     GSGELGQLDPSTGDTYHIHLGNGSAPHGVIVGPDGAPWITDGGLNAIVRVDPATKEIK
                     IFSLPADNVYANLNTATFDKSGILWFTGQNGIYGRLDPATGQIKIFSSPRGPGPYGIT
                     TTPNGEVYFASLAGSYIARIDLETGNVTVLEPPTLDQGARRVWSDSINRIWVSEWDSG
                     KLGVYNPDNKTWKEWQLPGENPLPYAVYVDERDMVWVSDFASNSFLRFDPVLERFEEF
                     KLPSKDGKVRQILGSLGEIWGAESGTDKL"
BASE COUNT          773 a          330 c          326 g          783 t
ORIGIN      
        1 agtttatctg tgccagactc tgcaccccaa atctctccaa gacttcctaa tatctgacgt
       61 actttaccat ctttgcttgg taacttgaac tcttcaaatc tttctaggac aggatcaaac
      121 cgtagaaatg aatttgatgc aaaatcacta acccatacca tatcacgctc atctacgtaa
      181 acagcatatg gcaatggatt ttctccagga agctgccatt ctttccaagt cttgttatca
      241 ggattgtaaa cgcctaactt accagaatcc cattcactta cccaaatcct gttgatagaa
      301 tcagaccaaa cacgtctagc tccttgatca agagttggtg gttccagaac agtgacattt
      361 cctgtttcta gatcaatgcg tgctatataa ctgccagcta aagaggcaaa atagacttct
      421 ccattaggag ttgtagttat cccatatggt ccaggacctc ttggagagga aaaaatctta
      481 atttgtcctg ttgcaggatc taagcgacca taaataccat tttgtcctgt aaaccataag
      541 atgccacttt tatcaaatgt agctgtattt agattagcat atacattatc tgcaggtaag
      601 gaaaaaattt taatttcctt cgtcgcgggg tcgacacgta caatggcatt caatcctcca
      661 tctgtaatcc atggtgcacc gtctgggcca acaataacac cgtgaggagc agatccatta
      721 ccaagatgaa tatgatatgt gtctccagtg cttggatcta attgtcctaa ttcacctgat
      781 ccttgggcag tataccaaat tgttccatct gctgcgggtg caacatcatg tggacgagat
      841 cccgaaggaa cgggaaattc ctttattaca aagttgttcg gttcaaaaga atttttgata
      901 gttgatatat tcttttcaat atttgttgat tttcctgtta gtataggtac agttgtgtga
      961 atgagaatat cttcttttac agagggattt gtagaagtta attgtctgtc tatattttta
     1021 tcttgtattt gttgaaaagc atatgctgga tttattagtt gtgcttgttg gaatccataa
     1081 tgagtcaata aaaataaact tgtaaaaaat aaaataatgt aacaataatg tataatcatt
     1141 atcaaaatat taacataatt tcttattaat tgtttacgtt gttatatatg attactcctc
     1201 tctaaattaa taagattaaa tttttatatc atttatttat ttattatatt aggatatcaa
     1261 ccagaaaaaa aaacactaat tggatgtaga tatattgtcc atattttttg attttgcatc
     1321 tttgatagga agataactaa gattaaagta attttatatg aatgctcata attgaataga
     1381 taagaaactt attgctaaaa gctatatata ttatgtaaaa tatttacatt tgaatctaat
     1441 aaaccaatta aatacttaaa cataactaaa taaaattatt acatctataa ttaactaaaa
     1501 attttttgat gaatatattt agatagttct tgtaatatta ttttatacta tgttgtatta
     1561 ttataaataa tgtcagaaat aaaagacgaa gtggaagatg caggagagaa gatgaaaaga
     1621 ggtgcaaaaa aagcaggaaa cagaatggaa gaaggcgctg atgaagcaaa agataaagta
     1681 gactaaataa cctaataaat aatgacatac tcttcttatt ttttacaata gcaatattgt
     1741 ttgaagagtg atcactaagg aattcatact attcaaatat tttaagaaat acaatgtata
     1801 atttaccttg aatatcccta taagtattat aacgttacaa tcataatgtt agttgaatta
     1861 ttagttgaac aattttttat tagactagat tattgaattt aaaattactt tatttacaca
     1921 tattattcaa actcttttgg gatttaatat tcctttatta gactaatgtt tgcaacatta
     1981 ttaaattaaa taacttacta ttattagtcg aatataatct tgattcaaat taaacctgca
     2041 ctattctact taactaaaaa aattgaatta tctgtattat aaataatatg ttttgaatct
     2101 cttaacatat tgttcaattt tgtttgtaat atctatgaat tggttcaatt tattgttgac
     2161 actaaaagta ataatatgag ataatttgtt tagacttaaa tttatctatt ac
//
LOCUS       DASVEV010000056         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_208690, whole genome shotgun sequence.
ACCESSION   DASVEV010000056 DASVEV010000000
VERSION     DASVEV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208690"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1011
                     /locus_tag="VF222_02920"
     CDS             97..1011
                     /locus_tag="VF222_02920"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7141412.1"
                     /translation="MDERNIIWIDGLLYESQNAKIHILTHSLHYGTAVFEGIRCYKTQ
                     KGLAIFRLEDHLKRLFESAKIYFMNIEYSKKELEDAIINTIKKNGLDECYIRPLVYFG
                     HGKMGVYPLNNKVSVSIAVWKWDEYIKKENDEQGIRLMVSGWMRIDGRTMPVHAKATA
                     NYANSALARIEAIKSGFDEAILLNTAGKVVEASAENIFIVKNKTLITPPVSSGSLEGI
                     TRDTVLALAREDNVLTEIRDISKDELYLADEIFLTGTAAEIKSVGEIDNRMIGNGKTG
                     TITKRMKRVFEKTIRGNHKYSEKWLKYI"
     gene            1081..1527
                     /locus_tag="VF222_02925"
     CDS             1081..1527
                     /locus_tag="VF222_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141413.1"
                     /translation="MKKVSTDLNLKNDYLDWDDIENLVKKTVLKIKKNNKKYDLIIGI
                     KNGGIIPAILISRELDINDIEFISIKRNKIFKFNKFHKNKKYLLIDEIYDTGKTFFIV
                     NEYFKRFEYDYACLVSRYRIPDNNNKIVSGKVLNHNKWIVFPWEKG"
     gene            1629..>2338
                     /gene="pstS"
                     /locus_tag="VF222_02930"
     CDS             1629..>2338
                     /gene="pstS"
                     /locus_tag="VF222_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7141414.1"
                     /translation="MRINQKIGVWLIAAVFFTANMPSLLEQTFAQTTINGAGATFPFP
                     LIDTWRVEYQNIKPDVNINYQSIGSGGGVKQFIEKTVDFGATDAPLNVEEEKKAVGAV
                     HIPETIGSVVVAYNLPQIADAELKLTGPILADIFMGKITKWDDPKIKEINSGLQLPSE
                     DIVVVHRSDGSGTTFVFTDYLANVSSGWETNIGKGKSVQWSTGIGAPGNEGVSGSIKS
                     TPNSIGYIELSYALTTDT"
BASE COUNT          939 a          261 c          422 g          716 t
ORIGIN      
        1 aatgctataa gagcaataca tttacaaaga aataaaagcg atttaaaaat tactcacaac
       61 aattaaactt tattagtatt aaagaatgaa aattaaatgg acgagagaaa tattatttgg
      121 atcgatggtc ttttatatga aagtcaaaat gctaaaatac atattttaac acattctttg
      181 cattatggta ctgctgtatt tgaaggaata agatgttata aaactcagaa aggactagcg
      241 atatttagat tagaggatca tttgaagagg ttatttgaaa gtgcaaaaat atattttatg
      301 aatattgaat attcaaagaa agagttagaa gatgcaataa ttaatacaat taaaaaaaat
      361 ggattagatg aatgttacat cagaccttta gtttattttg gtcatggaaa aatgggagtt
      421 tatccattaa ataataaagt ttcagtctca atagctgttt ggaaatggga tgaatatata
      481 aaaaaggaaa atgacgaaca aggaataaga ctcatggttt caggctggat gagaattgac
      541 ggacgaacta tgcctgttca tgcaaaggct acagctaatt acgctaattc agcattagct
      601 agaattgaag caatcaaatc tggatttgat gaagctatac tgttaaatac tgcaggaaaa
      661 gtagtggaag caagtgcaga gaatattttt atagtaaaaa ataagacatt aataactcct
      721 ccagtatcat ctggctccct ggaaggaata accagagata cagtattagc attggcaaga
      781 gaagataatg ttcttacaga aattcgtgat atatccaaag atgaattgta tttagcagat
      841 gagatttttt taactggtac agcggcagaa ataaagtcag tcggtgaaat tgataataga
      901 atgataggaa acggaaaaac tggaacaata acaaaaagaa tgaagagagt atttgaaaaa
      961 actataagag gaaatcacaa atatagtgaa aaatggttaa aatacatcta aataacttat
     1021 ataaaataat tattaaaaaa tgaataaata ttaattttta ttattatttt gattgattgt
     1081 ttgaaaaaag ttagtacgga tctaaatcta aagaatgatt atttagattg ggatgatatt
     1141 gaaaatcttg taaaaaaaac tgtcctaaaa ataaaaaaaa ataacaagaa atacgatcta
     1201 atcattggta taaaaaatgg tggtataatt cctgcaatat taatttcacg agaattagat
     1261 attaatgata ttgaatttat ttccatcaaa aggaataaaa tatttaaatt taataaattt
     1321 cataaaaata aaaagtattt attgatagat gaaatttatg atactggaaa aacttttttc
     1381 attgtaaatg aatattttaa aagattcgaa tatgattatg catgtcttgt aagtagatat
     1441 agaattccag ataataataa caaaatagta agtggaaaag ttttgaatca taataaatgg
     1501 attgtatttc cgtgggaaaa gggatagata tagtaagtta ttcatacatt atatttcatg
     1561 tagttgacta tagatctatg ttaaatatca tgtcaatgaa ttaattaaac ttattatgta
     1621 ctcaaactat gcgaatcaat caaaaaatag gagtttggct tatcgccgct gtatttttta
     1681 cagcaaatat gccatcactt ttagaacaaa cttttgcaca aacaactatt aatggtgcag
     1741 gagcaacctt tccatttcct ttaatagata catggagagt agaataccaa aacatcaagc
     1801 ctgatgtaaa tattaattat caatctattg gtagtggtgg aggagtgaag caatttatcg
     1861 agaaaacggt agattttgga gctactgatg cgccactaaa tgtagaggag gagaaaaaag
     1921 cggtaggtgc agtacatatt ccggaaacta ttggttctgt agtagttgca tataatttac
     1981 cacaaatagc agatgctgaa ttaaagctaa ctggacctat tcttgctgat atattcatgg
     2041 gaaagataac taaatgggat gatcctaaaa taaaagagat taattcagga ttacaattac
     2101 cttcagagga cattgtagtt gtacataggt ctgatggatc gggaactaca tttgttttta
     2161 cagattattt agcaaatgtt agctctggat gggaaacaaa tattggtaaa ggaaaatcag
     2221 tccaatggtc tactggcata ggcgctccgg gaaatgaagg agttagtgga tctataaaga
     2281 gtactcctaa ttcaattgga tatatagaac tgtcatatgc tctaactaca gatacaaa
//
LOCUS       DASVEV010000057         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1179961, whole genome shotgun sequence.
ACCESSION   DASVEV010000057 DASVEV010000000
VERSION     DASVEV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179961"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF222_02935"
     CDS             <1..523
                     /locus_tag="VF222_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7141415.1"
                     /translation="NIGKGKSVQWSTGIGAPGNEGVSGSIKSTPNSIGYIELSYALTT
                     DTKYAHIQNKDGNFIKPSLNSTAAAVSSFAPNLPNGDESWKDISIVNAPGKDSYPIAS
                     FSYLLLFKELSTNNSIDETKAKDLVDFISWAITDGQTFAPDLGYVALPNDIVKLDQET
                     LKSLTYKGSPILQ"
     gene            1008..1283
                     /locus_tag="VF222_02940"
     CDS             1008..1283
                     /locus_tag="VF222_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141416.1"
                     /translation="MKPNWVCSTCGMWSTRKFSVKRHITNQHSGNAFLVPYIDYLVGR
                     QSGLYPPSAYPEYQYKEKKSNLDTFSEEFWKEIARIAARRGNGNLQF"
     gene            complement(1770..2855)
                     /locus_tag="VF222_02945"
     CDS             complement(1770..2855)
                     /locus_tag="VF222_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX7141417.1"
                     /translation="MTTYMDGSEVRKIQFTGKSTFVLSLPKKWVNELNLKAGDPVTII
                     REPNNTLSIISDITRSSIEAVDEATVEVLRDEGGNSLKRKIVSMYLAGYNIINLRSKL
                     GRINPVQREGAREVVRRNLVGTEIIADSSDLITIQVLITLPELSVNTAVRRMFLLATS
                     MHKDAMTALSELNSELANAIIRSDDEVDRFSLYILRNLVIATKNERILNDVGLKKTSD
                     CLSYRLAVKSIERIADHASKFAEKSLSIKEKIPDDILHHIKKMSDLSLQDLNDSVEAF
                     LRRDYLMADKIVDRKENVQVLEDTIVSLLDNTDNGEFNQYKVFIKLILEDLRRTAEHA
                     SDIAEEALNQTIGEVINIEKRPSHYRT"
     gene            complement(2971..3897)
                     /gene="pstA"
                     /locus_tag="VF222_02950"
     CDS             complement(2971..3897)
                     /gene="pstA"
                     /locus_tag="VF222_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7141418.1"
                     /translation="MSSKTKENNHYIKRRKNIQEMMSKTSNRKIYVNKLVTILSICCI
                     IFAVIPLSSILFEVFKNGSSAISLEFLTQKPSSIGSGKGGIGPAIQGTLIVVSSASLI
                     AVPVGVFAGIYLAEYAKSNNKFAFAVRFFADVLTGLPSIIVGIVSYVIIVLSIGSFSV
                     IAGAFSLSLIMIPLVTRITEETLKIVPDSIREAAHSLGIPKWKITLSIVLKTATSGVL
                     TGIVLAVARIAGETAPLIMTILGTSLYFSSFTGPVDALPLRIWRLASQPYESAHAHGW
                     GAAMILIVLILGLNVALRVLARRQNMATSKDT"
     gene            complement(3901..4812)
                     /gene="pstC"
                     /locus_tag="VF222_02955"
     CDS             complement(3901..4812)
                     /gene="pstC"
                     /locus_tag="VF222_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7141419.1"
                     /translation="MIIIGAAAYTLVMLAIILFAVGEGSKEIFQEEGLNFLTNTDWNA
                     VPGRESFGALPYIIGTLVCSGIAMAIGVPISLGIAIFLSEMAGTKLKTSLSFIIELLA
                     AVPSIIYGFWALFVFRFWIMDFVEKPLHNLFGESLPFFAKTPFGLDVFTAGIVLAIMI
                     IPIISSIIRELLLVVPNSQREAAYSLGATRWETIKIAVLPYAKSGILGASIIGLGRAI
                     GETMLVTLIIGNATGLNAIPTSLFSQSQTLSSLIANEFNEAASELHLSALIGLGAVLL
                     LLTMVINVIARLLVYKMVKTSNPGGRI"
     gene            5495..>5862
                     /locus_tag="VF222_02960"
     CDS             5495..>5862
                     /locus_tag="VF222_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7141420.1"
                     /translation="MEIDESLINLINKKITGFKLLGIGSSLRKNSSSNKILKMVLEKS
                     QRYGANTSIIELSEINLPIFNPNKPKDISEDIKKINDQIINADGFILATPDYHGSMSG
                     GMKNFLDYYWYEFSGKIFGY"
BASE COUNT         2109 a          898 c          861 g         1994 t
ORIGIN      
        1 aaatattggt aaaggaaaat cagtccaatg gtctactggc ataggcgctc cgggaaatga
       61 aggagttagt ggatctataa agagtactcc taattcaatt ggatatatag aactgtcata
      121 tgctctaact acagatacaa aatatgcaca tattcaaaat aaagatggta attttataaa
      181 accatctctt aattcaaccg ccgctgcagt ttcttctttt gcacctaatc ttcctaatgg
      241 tgatgagtct tggaaagata tttccatagt aaatgcacca ggaaaggatt cttatccaat
      301 agctagtttt tcatacctac tgctattcaa agaactcagt acaaataatt ctatagatga
      361 aacaaaagct aaggatctgg tagactttat atcgtgggca attacagacg gacaaacttt
      421 tgcaccagac ttgggatatg ttgctttgcc taatgatata gttaaactag accaggagac
      481 attaaaatca ttgacatata aaggaagtcc aatactacaa taatctcttt ttaatttgtt
      541 cctttaattt aataagagag gtaatttctc ttagattatt atttattttt ttattattgc
      601 taagagagat taatttttat cgtataaatt gagataaaat atattgaaag ttataatttg
      661 ttgttttaat aattatttag tattcctcct atataatcta ggaaaatatg atgatgccta
      721 attatattga gaaatctcta aaaaaatgtt aatttaattg caatactgtc tgaaaagaat
      781 taactgtatt taataagcta atgaacaata caaataatag ataaatattt ctaataatgg
      841 acaaggtata ctttatattt caattaacta tttcttacaa tagcattttc tttaattcta
      901 ataaattatt ctagatgttc aataatttta aatacaatat tatattataa ataataacac
      961 agtgttgcac attgagacac agttaatcct aatggaggag attaaaaatg aaacctaatt
     1021 gggtatgttc tacatgtggt atgtggtcaa ctagaaagtt tagtgtaaaa cgccatataa
     1081 caaatcagca tagtggtaat gcattcttag ttccctacat tgactattta gtgggaagac
     1141 aatcaggact ctatcctcct tcagcttatc ctgagtatca atataaagag aaaaaaagta
     1201 atcttgatac atttagtgag gaattttgga aggaaatagc aaggatagcc gccagaagag
     1261 gaaatggcaa cttacaattt tgatataggt aattatctat aaaaattata tttaagcata
     1321 tgttgtggtg gttgttagaa ataccctatc gataatttaa tgcatttcaa caatcactgt
     1381 tgacaatctg aattattatc ttgtcataga tattacaaaa tgattccctc aggagattgc
     1441 gaaagtcaaa cagggaagca ttacataaaa catggatagg atactggata gctcaagata
     1501 aaggaaaata tcagacaaac ctattatgca acagtaatta gaaacttagt atcagatttt
     1561 gaatgcttac atcaagatgt cattaactac tatgataaag agcatcatat tttgtgattc
     1621 cattaagttt agtaaattta cgaattcaat tgaaaaacct tcaattctaa aagtgagcat
     1681 aaactataat gggaaaatat aacatatcta ctacttgtca ttagttaaga aatattatta
     1741 atgtatcaac atttgattta aatcattaat catgttctat agtgtgaagg tcttttttct
     1801 atattaatta cttcacctat tgtttgattt aaggcttctt ctgctatatc actcgcatgt
     1861 tctgcagtac gtcttagatc ttcaagtata agttttataa aaactttata ttgattaaac
     1921 tccccattat cagtattatc tagtaaagat acaatggtat cttcaagaac ttgtacattt
     1981 tcttttctat ctactatttt atctgccatt aaataatccc ttctcaaaaa tgcttcaact
     2041 gaatcgttca ggtcttgcaa agacaaatca ctcatttttt taatatgatg tagtatatca
     2101 tcaggaattt tttctttaat tgaaagagat ttttctgcaa atttggaagc atgatctgcg
     2161 attctttcaa tactttttac tgctagtcta taacttaaac aatcagaagt tttttttaat
     2221 ccaacgtcat ttaatatcct ttcatttttt gttgcaatga ccagatttct tagtatataa
     2281 agactgaatc gatcaacctc atcatcagat ctaataatag cattagctaa ttcagaattt
     2341 aattcagata atgctgtcat tgcatcttta tgcattgatg tagcaaggag gaacatcctt
     2401 ctaacagcag tattcactga aagttcggga agtgtaatca aaacttgaat tgttatcaaa
     2461 tcagatgaat cagcaattat ttctgtacca acaagattcc tacgaacgac ttctcttgca
     2521 ccttcacgct gtacaggatt aattcttcca agtttagatc taagatttat tatattatat
     2581 ccagcaagat acattgaaac tatctttcgt ttaagggaat taccaccttc atctcttaat
     2641 acttctaccg ttgcttcatc aactgcttca attgaggatc ttgtaatgtc agatattata
     2701 gataaagtat tatttggttc gcgaatgata gttacaggat cgccagcctt taaatttaat
     2761 tcatttaccc attttttagg taaggataat acgaatgttg acttacctgt aaactgtatt
     2821 ttccttactt cactaccatc catatatgta gtcaatagtt tatgaatagt aataagacat
     2881 gtatagttta tgattattta gaatatagct acatagtttt ctatatatta tatataggaa
     2941 atcaaaggtt tttagatcct aataattcaa ttaggtatct ttagaagtgg ccatattctg
     3001 tctacgtgct agcactctta aagcaacatt tagtcctaaa ataagaacaa tcaaaatcat
     3061 agcagcaccc catccatgag catgggcaga ttcatatggt tgagatgcca atctccatat
     3121 tctcagtgga agcgcatcta ccggtccagt aaatgatgaa aaatataaac ttgttcctag
     3181 gatagtcatg attaatggag cagtttctcc agcaattctt gctaccgcta atactatccc
     3241 agttagaact ccactagttg ctgtctttag tactatagat aaagtaattt tccattttgg
     3301 aattcctagt gagtgagctg cttctctaat agaatccggt acaattttaa gagtttcttc
     3361 cgttattcga gtaacaagcg gaatcattat taaagataat gaaaaggctc ctgctataac
     3421 agaaaaagaa ccaatagaaa gaactattat cacataactt acaataccaa ctataattga
     3481 aggtaatcct gttaaaacat cagcaaaaaa gcgtacagca aaggcaaact tattattgct
     3541 ttttgcatat tcggctagat aaattccagc aaaaacacct acaggtacag caataagaga
     3601 tgctgaactt actacgatta aagttccttg gatagctgga cctatacctc ctttacctga
     3661 cccaattgaa cttggttttt gagtaagaaa ttctaaacta atagcagaag aaccattttt
     3721 aaaaacctcg aaaagaatac tactaagagg aattaccgca aaaataatac aacatataga
     3781 taaaattgtt actaatttat ttacatatat ttttctatta gatgtttttg acatcatttc
     3841 ctgaatattc tttcgtcttt tgatataatg attattttct ttggttttag atgacatgta
     3901 ttatattcgt cctccaggat ttgaagtttt taccatcttg tagactaaga gccgggctat
     3961 tacatttatt accattgtaa gcaatagtag aacagctcca aggccaataa gtgcagaaag
     4021 atgtaattct gatgcagctt cattaaattc atttgctatt aaactagaca aagtttgact
     4081 ttgtgagaaa agagaagttg gaatagcatt caatccagtt gcattaccta ttattaaagt
     4141 aactaacatt gtttcaccta ttgctcttcc caggccaatt atactagcgc ctaatatccc
     4201 agatttagca taaggtaata cagcaatttt aatagtctcc catctagttg cacctaaact
     4261 atatgcagct tctctttgag aattaggtac aacaagtaat aattctctaa taatagaaga
     4321 gattatggga attatcatta tagcaagaac tataccagca gtaaatacat ctagaccaaa
     4381 aggtgtttta gcaaaaaatg gtaaagactc accaaataaa ttatgtaaag gcttttctac
     4441 aaaatccatg atccaaaatc taaaaacaaa caatgcccag aaaccatata ttatacttgg
     4501 aactgcggct aaaagttcaa taatgaagga taatgaagtt tttaattttg taccggccat
     4561 ttccgataaa aatatagcta tacctaaact aataggaaca ccaattgcca ttgctatacc
     4621 tgaacaaacc aaagtcccga tgatatatgg caaagctcca aatgattccc taccaggtac
     4681 tgcattccaa tcagtattag ttaaaaagtt taatccctct tcttgaaaga tttcttttga
     4741 cccctctcca actgcaaaaa gtattatggc taacatgacc aaagtatatg ctgctgctcc
     4801 aataattatc aatttgaaga taggatcaaa ttttgataat tttgttaatg gaataagaga
     4861 atgattgtca ttatacttag tcagattcgt gtcggatgtt gcgcctctct aataactatg
     4921 atattttgat atttttttag gtatcatata gttttaatat agatccatat agtctataga
     4981 ttagtatttt ctacaagggt ctcctcgtag taatatcgac aacattttat tatatatttc
     5041 ctgctgttga aagggagtag gtttgaaata agattctggc gagttatctt ttcatctttt
     5101 ataattatat aatgcataaa aaaaaagtac atcgcatgat gatgttttgt tttctacgta
     5161 attaacatca tagttcttaa aaatgtatat taaatatttt ttccaagtag tggtaagcat
     5221 gatagttatt acctttaatc tttatttctt acctcgattt tgaaaatttg actaattaga
     5281 aggctcaatt agaatgcagt ataatatttc aaaaaaaggg aagaatctat attattaata
     5341 atcaatttat attaaaataa aaagaaaatg tgttaaattc gaattttatt aacatcatat
     5401 ctccagtatt taaggacgat atgaaaatta aataaatatc tctactatga aagcttgaac
     5461 gaataaatta aagagaacag aaaaagtaaa aaaaatggaa atagatgaat ctttgataaa
     5521 tttaattaat aaaaaaataa ccgggtttaa gcttttaggt ataggttcta gtttacgaaa
     5581 aaattcttca agtaataaaa tattaaaaat ggttttagag aaatcacaaa gatatggagc
     5641 aaacacaagc ataatagaat tgagtgaaat taacttacct atttttaatc ctaataaacc
     5701 aaaagatatt tctgaagaca ttaaaaaaat aaatgatcag ataataaatg ctgatggatt
     5761 tattctagct acgcctgact accatggatc aatgtctgga ggaatgaaaa attttttaga
     5821 ttattactgg tatgaatttt caggaaaaat atttggatat at
//
LOCUS       DASVEV010000058        22372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_792320, whole genome shotgun sequence.
ACCESSION   DASVEV010000058 DASVEV010000000
VERSION     DASVEV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792320"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1391
                     /locus_tag="VF222_02965"
     CDS             150..1391
                     /locus_tag="VF222_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7141421.1"
                     /translation="MYWAEIYRPKNLEDILGNEENRLNILKWLVKWFPGTKPLLLIGP
                     PGVGKTTIVKILSSLLNYDLIELNASDTRSGPQLEKLINPLHNNTSIFGKNILLFFDE
                     VDGIYGREDSGGLEILINIVKASNFPIIFVANYAHIKLKNLSKLCKIIKFQRISENLL
                     MIFLNYVLFRQNYPLSLDDKKWIVEKSNGDVRTMLNLAQSKCTGYRKFVHETFKIDIS
                     EGLNEFFQSKNKDEARKILLSLDGKYSDPRYGLSSEERRKDILNSLFTSIVSSNPDMD
                     TLSLLLDSLSQIDLLVARINKNRNWNLLKYMNYVIIEILYENIQNKMVSYHQYGISWP
                     LMGQIFSRGVPLRSLISKLALSFHVSSSTFGMIFFPYLLEIIHEFKIDIHELITNLLL
                     DEKFVEIIQKEILRSKRYERI"
     gene            1378..1758
                     /locus_tag="VF222_02970"
     CDS             1378..1758
                     /locus_tag="VF222_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141422.1"
                     /translation="MNESDWIKLLVKFKGKCQSCDNPISSGEYALWSKSSKKIKHLNC
                     KTNNNKTIATEQVEKYSGLQLRCFLCNTVDEISIHLLNEFLYHEGQEGSIFLCKSCLE
                     NPKSYLRYREIMYEKVKKIAKLKS"
     gene            1758..1991
                     /locus_tag="VF222_02975"
     CDS             1758..1991
                     /locus_tag="VF222_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141423.1"
                     /translation="MNTNTRKMSSEYEHNIAKLLFEKKQKLDQLLENPEKNKYQIQLL
                     SNEIETLKTLFENYNLGLNVFRRAQGGRSLLRE"
     gene            2124..3665
                     /locus_tag="VF222_02980"
     CDS             2124..3665
                     /locus_tag="VF222_02980"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7141424.1"
                     /translation="MHEEKLKKLYELKNLAESGGGKERIDAQHSKGKLTARERIDLLL
                     DEGSFIELDKYVTHRNDDPETQKFYGDGVITGFGSINGRKIFVYAYDFTVLGGSLGEM
                     ASRKIVKIMDRAIKVGAPLIGIIDSGGARIQEGVMSLDGYGDIFYRNCTASGIIPQIT
                     ASIGPCAGGAVYSPAMTDFVIMVENIGQMFVTGPEVVKEVLSEDVSFEELGGTSTHAT
                     KSGVAHMVAANEYECMEKIKKLISFLPQNNLQEPPTLETNDDPNRLDPSLIDMLPENP
                     YQPYDIKSIITSIIDDKEFFEIHETFASNIVVGLARMGGKTVGIVSNQPMYLAGVLDI
                     DSSNKAARFIRFCDSFNIPIITLVDTPGYLPGTDQEQNGIIRHGSKLLYAYCEASVPK
                     ITCIIGKAYGGAYIAMGSKNLKTDINFAWPSAEIAVLGPEAAITIIHRRELKSSLDAK
                     TIKKKLAEEFRKKFANPYIAAEKGIIDQVIDPMETRTMIIGALDSLSGKREIGPWKKH
                     GNINL"
     gene            3678..5177
                     /locus_tag="VF222_02985"
     CDS             3678..5177
                     /locus_tag="VF222_02985"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7141425.1"
                     /translation="MPKISSILIANRGEIALRVIRACKEMGIKSISVYSDDDNRSIHV
                     KRADETYRLGPPQASESYLRIDKIIEIAKSSGADAIHPGYGFLSENEVFSDECKKNGI
                     IFIGPTARAMELAGDKMKCKEIMKKAEVPIAPGSDGVVDDIELAVEIAKEARYPVMLK
                     SAFGGGGRGIRLVNNEKELRQEFELASNESTAAFGKSALFIEKYLTNIRHIEFQLIRD
                     SSGNGRHLFERECSIQRRHQKLIEMSPSPVVNKERRERIGEIAVRAGTALDYLNAGTV
                     EFLADAEGNFYFIEINARLQVEHPITEIVTGKDLVKLQIHVAQGEELPFKQQDLELKG
                     SAIECRINSEDPFYDFAPSVGYVPNCNIPAGPGVRVDTYLYPGCNVSGYYDSLIAKLI
                     TYGGDFNEARIRMKNALPEFTIEGINTTIPIHKTILEESNFINGNISTDYIEKYNIIE
                     KMKHKRDQEYKDKAHAAISGILLQSEFVKKSNLGQSIKRNQKSMDGGFF"
     gene            5177..5689
                     /locus_tag="VF222_02990"
     CDS             5177..5689
                     /locus_tag="VF222_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX7141426.1"
                     /translation="MQFQIEGSPNKINAELIYNKGSGDLLFVINDQKYNLKLLKSTSN
                     EFEFILENTYHYVKILPSTETKYKIYIDGIVIEIKKHSKITEIIEKTLKSKGAINQAN
                     NVTSQIPGRVVKVSVSNGDKIKEGDPIVVLESMKMQIAIKSHKEGTIKEIKVKEGSTV
                     ARNEIVAIID"
     gene            complement(6218..7423)
                     /locus_tag="VF222_02995"
     CDS             complement(6218..7423)
                     /locus_tag="VF222_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7141427.1"
                     /translation="MIKNKSNNHLIYLDHAASTPVLQEIINEMIPYLGNLYGNPSSIH
                     TYGIKSKIAVQIARRRVASLIGAKPSEIFFTSGGTESDNLALKGICKSIRKSQNIKNH
                     IITSSIEHDAILETCRYLEKDGFTVTYLNVDKNGIIDKTELKNHLTEKTALVSLMLAN
                     NEIGVIQPVKEFSETVHAFDKMIKFHSDAIQAVGKIPINVKELGLDSLSLSSHKINGP
                     KGVGALYVREKVNFEPLIQGGGQELTIRSGTENVPGIVGFGKASEISMINLKDNSQYL
                     YSLRDYLIKRINEEISGKMLNGSLESRLPNNVNFTFLGINGEDLLIKLDEDGILASTG
                     SACSANRQKESHVLKAIGLNHEEISGSIRFTLGIQNTIEDLEKTIIVLKNRITELRKF
                     SPMKSRSEK"
     gene            complement(7425..8240)
                     /gene="larE"
                     /locus_tag="VF222_03000"
     CDS             complement(7425..8240)
                     /gene="larE"
                     /locus_tag="VF222_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX7141428.1"
                     /translation="MNSEKSFLQLKDWFKKNTKGNVFIALSGGVDSAVVALAAKKTLG
                     EKAIAITANYKTLALLELEDAKRVANEIGIQHIIIHYNELEDENFVKNDINRCYYCRK
                     SLGENIKRYEKEYQVTDVVDGTHLDDMNDYRPGLKAMREYKIKSPLLEKRIGKKEIRE
                     IAKRFNISVANKPSNSCLASRIPYGNNITQEKLKKIEIAEEKVKELFDISHVRVRDHG
                     EIARIEIGRDEMEKMFNKIKLNQLDLELKNIGFKYASLDLYGYRSGKLIVIND"
     gene            complement(8233..9486)
                     /gene="larC"
                     /locus_tag="VF222_03005"
     CDS             complement(8233..9486)
                     /gene="larC"
                     /locus_tag="VF222_03005"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX7141429.1"
                     /translation="MSKISIIDAQLAGLSGDMMLSALIDLGADKQKVIDSIYTCENIM
                     SGSKIKDVRFEKVNSKGFTATRFYFEYREEIQERKGIEILNNISKSLEYLDLKPRPKS
                     FIVDSIKTLIDAEAKIHGEPVNQVYLHEASSIDTFADLIGCAVALEDLKVFDSKIVST
                     YISIGNGLTNFSHGTIPNPTNAILEIFKGKSFVLTGNDQGELTTPTGAAMLVNLSSEC
                     IRYYPPIIPEKIGLGAGHRKLKDRPNVLRIVLGKDPVDSPLTNEKLFLIETNIDDVDG
                     EIIGNLIEVLMEEGARDVTIIPGITKKNRPVNIIRVLSDINKSDSLIERLFKETGTSG
                     IRINEINRITLERNIIIIQVTVNHSKYDIKVKITRDSSNNIINIKPEFDDLKKISDIE
                     DSSLKAIQELVMYQIFKKFKGNSYE"
     gene            9638..10555
                     /locus_tag="VF222_03010"
     CDS             9638..10555
                     /locus_tag="VF222_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7141430.1"
                     /translation="MTITIVGSGRVGASVALNCGLRELDDIILLDVIEGLPQGEAMDI
                     NHQLSELGIDSKVKGSNSYEDMKNSDFVVLVAGLGRKPGMTRMDLLNKNASIVKDVAT
                     KISTYANNATLVVVTNPLDPMTYFALKTTRFDKNKVIGMGGMLDLSRFKSFIHDASGC
                     SRASIQAMVISEHGENMLPLIRFSSIGGIPINEFLSKEQSQEIFEKTKKVAAEVIALK
                     GATVYAPGNAVATMIESMKKNKKEVIPVSAYLEGEYGISDLCIGVPVVLGSNGIEKII
                     ELELNEFERKIFDLGVTSVREAINLYTQK"
     gene            10560..11348
                     /gene="larB"
                     /locus_tag="VF222_03015"
     CDS             10560..11348
                     /gene="larB"
                     /locus_tag="VF222_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX7141431.1"
                     /translation="MEFEELLTQYNKGNISINELKRSITSSYIYNVGKNIAKLDINRS
                     YRKGIPEVIYAANKDYHDIEKIILNMLEKNNTIVVSKIQKEHIKKLITFVKKKNFYVE
                     IGRSSSTIFLSKNSKFKYHYYKIGIISGGTSDIGIAEESRLMAKAMNCDAIISYDIGI
                     AGIHRVFPTLDKMISENVQAIVVVAGMEGALASLVSSCVNVPVIGVPTSVGYGFGSNG
                     IAALSSMLQSCALGLTVVNIDNGIGAGSFAALIANRMSNMNKIK"
     gene            11428..12090
                     /locus_tag="VF222_03020"
     CDS             11428..12090
                     /locus_tag="VF222_03020"
                     /inference="COORDINATES: protein motif:HMM:NF006342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7141432.1"
                     /translation="MNYIHSLKRIRERKTNYHKRASLLLSKKKFITIKVTNQNILTQV
                     LEPSITGDKVITSAHSTNLQKLGWKGSLNSLPACYLTGLLLGKKAVFKGIDNAILYTG
                     KDSFTERISACLKGIIDGGMEIPSSEDAFPSTERISGEHISTYASALKSDSERYNKRF
                     SSLIKNGLNPENYKTHFEESKEKIMKLEIQNFIKNENNKVPYPNKNKIELVDQTQGEE
                     SK"
     gene            12091..12714
                     /locus_tag="VF222_03025"
     CDS             12091..12714
                     /locus_tag="VF222_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7141433.1"
                     /translation="MSHHQFRREEKQVEWKPRTTLGMLVAGGKISSMEQVFENGMRIQ
                     ESEIVKQLLPDMKSQVVHVGIVQKQTDAGEMTRFAALVAVGNEGGWFGIGTGKAAQMR
                     NAIDKATTASYLNVIPIKLGCGSWECRCNQLHSIPFQVSGKGGSVTLEILPGPRGLGL
                     VAGEDIKKLLKLAGIKDAWTKSYGSTNTMASTTKAIFNALRSTYTAG"
     gene            12724..13182
                     /locus_tag="VF222_03030"
     CDS             12724..13182
                     /locus_tag="VF222_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7141434.1"
                     /translation="MVYLVIRIRGIVNIPSWAEKTLDNLNLRKKFNATIVTENSQTLG
                     MLRKVKDIVAWKSVDESIIKELLEKRGRKKGLHVLTNSSLPSNYKTIDDLANAIHKNN
                     ISLSELEDLKPWFSLSPPKGGFRRKTKTQYTQKGTVGENNELVDLVRRMI"
     gene            13279..13716
                     /locus_tag="VF222_03035"
     CDS             13279..13716
                     /locus_tag="VF222_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEX7141435.1"
                     /translation="MATRFRKSRRQRGTRYCGWGQIGQHRQSGSRGGVGQAGKHKHFW
                     IRTVIEEPDHFGHPQLKSHHKIDVKKWANLRDLDILYLKHSNNKLNENETLDLTALGI
                     NKLLGAGSVTTKMTVKVEQISRRAVEKIKAIGGNVISNEHSSE"
     gene            13697..15130
                     /gene="secY"
                     /locus_tag="VF222_03040"
     CDS             13697..15130
                     /gene="secY"
                     /locus_tag="VF222_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7141436.1"
                     /translation="MNTVQNEGFIRKSIKTVSRYIPQVEKPKKKISLTEKFVWCGIAV
                     FAYLVMGQVPLYGVTDSPQFDFLAFARVIFAAQQGTILELGIGPIVTAGLLMQLLKGS
                     DILKLNFKNPDDRALFTSATKIVTIIVIIAEGGLYGASVYGPLVASPSFIPILIAQLI
                     FTSLIVMLLDELIQKGWGLGSGISLFIMAGVAQTILWSVFSPVAGPDGPVGIIPFVID
                     SAATGDLSDTFFRSGQLPSIFTFILTGGVLALLVYTQGIHIDIPIVSTKYRGFTAVYP
                     IKLLYVSNIPVILSTALIANAMFMGQMLWANYNPQNDNPFFNWIAQFDPGQQQTPTGG
                     LLYYMSTPRGLDIVAQDPIRALTNIIFVTAIVTVFGRLWVELGGLSAKAAAKNLLDAD
                     VQIPGFRRSQNSVQNVLDKYIPSVTILGGIAIGLLASVSDVLGLFGGGIGLLLMVDIL
                     VNYYNLLIREKVDTTMPKLAALIGRKD"
     gene            15114..15698
                     /locus_tag="VF222_03045"
     CDS             15114..15698
                     /locus_tag="VF222_03045"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7141437.1"
                     /translation="MEEKTKRIIVVGIPGVGKTTVVSSVKNTLEQTGIKTTLAEFGKI
                     MLEEAKKMKINNRDQIRRLSIENQKKLQNLAAEKISQIYSDVVIIDTHLFINTKEGYY
                     PGLPADLLNHLKPTHLILIIANPEEIYHRRQNDSSRVRDIISMDSIKNEIDVSKMLIS
                     CCSIISGAPFKILANHEGLVTDCTQKMVSLILDT"
     gene            15714..16313
                     /locus_tag="VF222_03050"
     CDS             15714..16313
                     /locus_tag="VF222_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HEX7141438.1"
                     /translation="MYLSDLILQIPGLTPAYTKHPIFPDLFSAMIVAALISIGLNFAF
                     AILRKKTTDIDRLSKVMKETNEWRKEYTDAIRKQDKVRISELKKKQAYVNKMSLEMQQ
                     QQMRPMMLYMIPSFMLWILVFPTIFGQTVALSPLHIPYLMCTDENVKKDSVLDANGQP
                     TGVCNIPGEVFLWGWFLITSLGTSGLIAKITKTSMPSMT"
     gene            16315..16905
                     /locus_tag="VF222_03055"
     CDS             16315..16905
                     /locus_tag="VF222_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX7141439.1"
                     /translation="MLTSNYKNKILNSIVISGWPAVGKTTIATELAKEFGYKHYNGGD
                     VLKMLAAEKGYIVSGNDWWDTEEAKNFMEERESNSYFDIEVDRKLVEIVRMGKVVVTS
                     YTLPWLVPESIKIWLKGSRSNRIKRMAKRDSLNLKHARNVIQQRDENNVRIYNNLYGY
                     EFGKDLSVFDFVLDTDLLNLDSLLLISKTIVKNLRV"
     gene            16971..17969
                     /locus_tag="VF222_03060"
     CDS             16971..17969
                     /locus_tag="VF222_03060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HEX7141440.1"
                     /translation="MDLKRNKKLIQFDNLIRVSESVTDDRYGYFPYDRPIHILFDYGL
                     ILIDKPSGPTSHEVASWVKRILNIDKSGHSGTLDPGATGLLPMGLGEGTKALSILLLG
                     PKEYYSIARIHSNFDQKILENVLLEFHGDIYQRPPQRSSVKRMTRIRRIHELEYVEED
                     DKLILLRILCQAGTYIRKLIYDIGEVMGTGATMVELRRTRVSHFFERDGLIKLHDLYR
                     AFYLYKEKNDEERLRNIVNPIEICFKEIPSVVIRDTAVDALCHGAQLAIPGISELSPS
                     IQKDDLVAIYTLKGEIVGLAQSLMNINEIVSNKNGISFTMKRLIMKPNTYPKGWRS"
     gene            complement(18038..18196)
                     /locus_tag="VF222_03065"
     CDS             complement(18038..18196)
                     /locus_tag="VF222_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141441.1"
                     /translation="MKSAEISQYCKRNNHIECSDCMCKCHIPGTMENLAKKIANLDRK
                     TPGIGETI"
     gene            18335..18428
                     /locus_tag="VF222_03070"
     tRNA            join(18335..18372,18383..18428)
                     /locus_tag="VF222_03070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18369..18371,aa:Ser,seq:gga)
     gene            19502..20020
                     /locus_tag="VF222_03075"
     CDS             19502..20020
                     /locus_tag="VF222_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141442.1"
                     /translation="MNNKLLPINSDDNKDKTNKAKIYNYKSTEIVNKEKIKKLKIHID
                     GVNKISNKKNIIHKKIQLWTNDKGNEYAICLLFDSDVKIDNAQFIASTNPNEKYIHPI
                     AETDNIKDLQHGWDYTLREVIENYKKNYYRKISGEDIQSIKLIPFYKCKYCNKELSSE
                     DEKKDHEFLWHI"
     gene            20564..20734
                     /locus_tag="VF222_03080"
     CDS             20564..20734
                     /locus_tag="VF222_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141443.1"
                     /translation="MTDKNWITPSANITSVNTHEVIVATDTVDSILSDPQHPPSSKIS
                     YLHDIRRRMKIC"
     gene            21558..>22372
                     /locus_tag="VF222_03085"
     CDS             21558..>22372
                     /locus_tag="VF222_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX7141444.1"
                     /translation="MENVCILGAGSTKYGKLNESIIEMALSASVDAIESAGISPNDIQ
                     AGYISNVFGVADKQVHIAPVIMSNLGISHVPGLTIESACGSGSVMFREAFANIRAGFY
                     NCVLALGVEKITHTGTTQSTTLFSYCSDFFYEGGNGASFPGLFASIARAYMTKYKADE
                     EDLAHVAVKNHENGLLNPKAHVRKKITVEDVLKSPVVASPLKLYDCCPFSDGASAVIL
                     CSEDFAKKSGKPYIQVVGSGRGGSPAAVQGREDISTIPGTVIAANQAYKMAGIT"
BASE COUNT         7857 a         3270 c         3833 g         7412 t
ORIGIN      
        1 aaccattttt aatgttttta tttcttacag taaaccacta taatcagtat tggtatcatg
       61 atatcacaaa acgtgacctt tttaaatatt tcgaaaggac tatccatccc ctcacacgaa
      121 agttcattta atttatatta tgatatagta tgtattgggc agaaatatat cgaccaaaaa
      181 atttagaaga cattcttggc aatgaggaaa ataggcttaa tatactaaaa tggttggtaa
      241 aatggtttcc tggaacaaaa cctttacttt taataggacc accaggcgtt gggaaaacta
      301 cgattgttaa aatattatct tctcttttaa actacgatct catagaatta aacgcaagtg
      361 atactagaag cggtcctcag ttagaaaaat taataaatcc tcttcataat aatacgagta
      421 tttttggaaa gaatattctt cttttttttg atgaggtaga tggtatttat ggaagggaag
      481 atagcggcgg actcgaaatt cttattaata ttgtaaaagc atcaaacttt ccgataatat
      541 ttgtggctaa ttatgctcat attaagttaa aaaatttatc caaactatgt aaaataatta
      601 aattccaacg tatatcagaa aacctcttga tgatattttt aaattatgta ttatttagac
      661 aaaattatcc tctctcttta gatgataaaa aatggatagt ggaaaaaagt aatggtgatg
      721 taagaacaat gcttaattta gctcaatcta aatgcacagg ctaccgaaag tttgtgcatg
      781 aaacatttaa aatagatatt tcggaaggtc taaatgaatt ttttcaaagt aaaaacaaag
      841 atgaggccag aaaaattttg ttatctttag atggcaaata ttcagatcct cggtatggtt
      901 tatcttctga agaacgaaga aaagatattc tcaattcgtt atttacaagc attgtatcct
      961 ccaatcctga tatggatact ttgtctctac tattagatag tctttcccaa attgatttac
     1021 ttgttgcacg gataaacaaa aatagaaact ggaatttatt aaaatatatg aattatgtaa
     1081 taattgaaat tttatatgaa aatatacaaa ataaaatggt atcttatcat caatatggaa
     1141 ttagttggcc tcttatgggt cagatatttt ccagaggggt acctttgcgt agtttgattt
     1201 ctaagctagc tttatcgttt cacgtttcat ccagtacttt tgggatgata ttttttccat
     1261 atctgctgga aattatccat gagtttaaaa tagatatcca tgagttaatt acgaatctct
     1321 tgttagatga aaaatttgta gaaattatcc agaaggaaat cttgaggtct aaaagatatg
     1381 aacgaatctg attggataaa actattggta aaatttaaag gaaaatgtca aagctgtgac
     1441 aatccaattt catcaggtga atatgcttta tggtcgaaat catcaaaaaa aattaaacat
     1501 ttgaattgta agaccaataa caacaaaacg atagctacgg agcaagttga gaaatattcc
     1561 ggattacaat taaggtgttt tttgtgcaat actgttgatg agatctccat tcatctatta
     1621 aatgaatttt tatatcatga aggtcaagaa ggttcgatat tcttatgtaa atcttgtttg
     1681 gaaaatccta aatcgtattt aagatatcga gaaataatgt atgaaaaagt aaaaaagata
     1741 gcaaaattaa aatcttgatg aatacaaata caaggaaaat gtcaagtgaa tatgaacata
     1801 atattgcaaa attgctcttc gaaaaaaagc aaaaattgga tcaattatta gaaaatccag
     1861 aaaaaaataa atatcaaata caattattgt ctaatgagat tgagacatta aagacattat
     1921 tcgaaaacta taatcttggt ttgaatgtct ttagaagagc acaaggtgga cgctccctct
     1981 tacgtgagta acagttactt tatttaagat aattattagt taattgtaat aaaatttttg
     2041 tgaatccctg tcccgtaaat cttttttcgt cactattttt tgttatatat atatactaga
     2101 ttatcgggat aaatgctgaa gccatgcacg aagaaaagtt aaaaaaatta tacgaattga
     2161 aaaatttagc tgaatcgggg ggtggtaaag agagaattga tgctcaacat tctaaaggaa
     2221 aactcacagc tagagaaaga attgatcttc ttcttgatga agggagtttt attgaattgg
     2281 acaaatatgt gactcataga aatgatgatc ctgaaactca aaaattctat ggagacggcg
     2341 taataactgg atttggttca attaatggta gaaaaatctt tgtgtatgca tatgatttta
     2401 cggttttggg tgggtcattg ggagaaatgg catccagaaa aattgtgaaa atcatggata
     2461 gagccattaa agtaggtgca cctcttatag gaattattga ttcaggtggg gctaggattc
     2521 aggaaggagt aatgagttta gatgggtatg gtgatatatt ttacaggaat tgcaccgctt
     2581 ctggaataat tccgcagata actgctagca ttggtccttg tgctggtggt gctgtatatt
     2641 ctccagcaat gacggatttt gtaataatgg tagaaaatat aggtcagatg tttgtgactg
     2701 gaccagaagt ggtgaaggaa gttttaagtg aagatgtctc atttgaagaa ctcggaggta
     2761 cttctacaca cgcaacaaaa tctggagtag ctcatatggt tgcggctaat gagtatgaat
     2821 gtatggagaa aattaaaaaa ttaatatcgt ttttacctca aaataattta caagaaccac
     2881 ctaccttaga gacaaatgat gatcctaata gactggatcc atcattaatt gatatgctac
     2941 ctgaaaatcc gtatcagcca tatgatatta aatcaatcat aacaagtata atcgatgata
     3001 aagaattttt tgaaattcac gaaacttttg caagcaatat tgttgtaggc ttggcaagaa
     3061 tgggtggtaa aaccgttgga attgtatcca accagcctat gtatttagca ggtgttttag
     3121 atattgattc ctctaataaa gcagcaagat tcattagatt ttgcgattct tttaatattc
     3181 ctattattac attagttgat acacctggtt atttacctgg aaccgatcaa gagcaaaatg
     3241 gtattattag acatggtagt aagttattgt atgcttactg tgaggcttca gtacctaaaa
     3301 ttacatgtat cattggaaag gcgtatggtg gagcatatat tgccatggga agtaagaacc
     3361 taaaaacaga tattaatttt gcatggccct ctgctgaaat tgctgtttta ggtccagaag
     3421 cagcaattac aataattcat agaagagaac ttaagagttc tctagatgca aaaacaataa
     3481 aaaagaaatt agcagaagaa tttagaaaaa aatttgctaa tccatatatt gcagcagaga
     3541 aagggatcat tgatcaggtt attgatccaa tggaaactag aactatgata ataggtgccc
     3601 ttgactcatt atccggaaaa agagagatag gtccttggaa aaaacatgga aacataaatt
     3661 tataggtgaa cttgagaatg ccaaagattt ctagtatcct tatagcaaat cgtggagaga
     3721 ttgcgctgcg tgttataaga gcgtgcaaag aaatgggaat aaaatcaata tcagtttatt
     3781 cagatgacga taatagatca atccatgtta aacgagctga tgaaacatat cgattaggcc
     3841 caccacaagc ttcagaaagc tatcttcgta tagacaaaat aattgaaatc gctaaatcct
     3901 ctggggccga tgctatacat cctggttatg gatttttatc agagaacgaa gttttttcgg
     3961 atgaatgtaa gaaaaatggc attattttta taggaccaac agcaagagcg atggagttag
     4021 caggtgacaa aatgaaatgt aaagaaatca tgaaaaaagc ggaagttcct attgcaccag
     4081 gaagtgatgg agtagttgat gatatagaat tagctgtgga aatagcaaag gaagcacgtt
     4141 atccggtaat gctaaaatca gcttttggag gaggaggacg gggaattaga ctagtaaata
     4201 atgaaaaaga acttaggcaa gaatttgaac tagcgtcgaa tgaatcaaca gcagcctttg
     4261 gtaaatctgc actatttatc gaaaaatacc taacaaacat tagacacatt gaatttcaat
     4321 tgataagaga ttctagtgga aatggtagac atcttttcga aagagaatgt tctattcaaa
     4381 gaagacacca aaaacttatt gaaatgtctc cttcccccgt tgtaaataag gaaaggagag
     4441 aaagaatagg cgaaattgcc gttcgtgcag gtactgcact tgactattta aacgcgggta
     4501 cagttgaatt tttggctgat gcggaaggaa atttttattt tattgaaatt aatgctagat
     4561 tgcaagtaga acatccaatt accgagattg ttaccggcaa ggatttggta aagttacaaa
     4621 ttcatgtcgc tcaaggggaa gaattaccat ttaaacaaca ggatctcgaa ttaaaaggct
     4681 ctgcaataga atgtcgtatc aattcagaag acccttttta tgatttcgct ccttctgtag
     4741 gatatgttcc aaactgtaat attccggctg gcccgggcgt gagagttgat acttatctct
     4801 accctggttg taatgtttct gggtattatg attcattaat tgctaaatta attacttatg
     4861 gtggagactt taatgaagca cgcatacgaa tgaagaatgc tttacctgaa tttaccattg
     4921 aaggaataaa tactacgata ccaatccata aaacaatttt agaagaatca aattttatta
     4981 atggcaatat ttctactgat tatatcgaaa aatataatat aatcgaaaag atgaaacata
     5041 aaagagatca ggaatacaaa gacaaagcgc atgccgcgat ttctggaatt ctattacagt
     5101 ctgaatttgt aaaaaaatct aatttaggtc aaagcataaa gagaaatcaa aagtcaatgg
     5161 atggaggatt tttttaatgc agtttcaaat agaaggtagt cctaataaaa ttaacgctga
     5221 attaatttac aataaaggat ctggtgatct tttatttgtg ataaatgatc agaaatataa
     5281 tttaaaactt ttaaaatcca catccaatga atttgaattt attttagaaa atacatatca
     5341 ttatgttaaa atacttcctt ctacagaaac aaaatataaa atttatatcg atggaatagt
     5401 aattgaaatt aagaaacatt caaaaataac agaaataatt gaaaaaacat taaaatcaaa
     5461 aggagctata aatcaagcta ataacgtaac tagccaaata cccggtagag ttgttaaggt
     5521 ctctgtttca aatggtgata aaattaagga aggagatcca attgtggtcc ttgaatctat
     5581 gaaaatgcaa attgctatta aatctcataa agaaggtact ataaaggaaa tcaaagtaaa
     5641 agaaggatca actgtggcaa gaaatgaaat tgtagcaatc atagattagt cggcttcata
     5701 tttcaaaata tttattacac ataaaaatga tgtatacaag atcattatta ccaggaacaa
     5761 taagcaggtt atctgaaatt tacttatgta aacttattat ctaatataaa aaaaggattt
     5821 tcacatcttg tataatttta agctatggta gaggctataa tctattatgt atctttatta
     5881 tctatctatt gtagtcactt aataaatggg aaatttaaat ccagtcttca ttgatgaatt
     5941 agatgataaa gaatcattaa taaatagttg aattacttga acatttatac tatttaatca
     6001 ctgagataaa tctaaagcaa gacatttcag aattacaaca ttacaaaggt gatattctgc
     6061 ggttggaaaa aaagcaaata cgatatttta tcatttatag cgcttcattt tatgtttttc
     6121 atttctcatt aactaattca aaatgagtta gccatttact acatttgaaa atattctcca
     6181 atcagcacat actaatcttg ataagtttaa attcatacta cttttcagat ctagatttca
     6241 taggagaaaa ttttcggagt tctgtaattc tatttttcaa aactataata gttttttcta
     6301 gatcttcaat ggtattctgt attcctaaag taaacctaat tgaacctgaa atctcttcat
     6361 gatttaaacc tatagctttc aaaacatgag attctttttg tctgttggca gaacaagcag
     6421 atcctgtaga agctaagatt ccatcttcgt ctaatttaat tagtaaatct tcaccgttta
     6481 tccctaaaaa ggtaaaattg acattattgg gtagtctact ttcaagagaa ccatttaaca
     6541 ttttaccaga gatttcctca tttattctct ttattaggta atctctcaaa gaatacaagt
     6601 actgggaatt atcttttaag tttatcatgc ttatttcaga agcttttccg aaacctacaa
     6661 taccaggtac attttcagtt ccagatcgaa tggttaattc ctgtccacct ccttgtatca
     6721 aaggttcaaa atttactttt tcccgaacat ataatgcacc tactcctttg ggtccattga
     6781 ttttatggga agacaatgat aatgaatcca gacctaattc tttaacattt atcggtattt
     6841 tgccgactgc ttgaattgca tccgaatgaa attttatcat tttgtcaaag gcgtgaacag
     6901 tttctgagaa ttctttaact ggctgaataa cacctatttc gttattagct agcattagac
     6961 tgactaaagc agttttttca gtaagatggt ttttgagttc agttttatcg ataataccat
     7021 ttttatctac atttagataa gtaacagtaa agccgtcttt ttctaaatat cgacaggttt
     7081 ctaagattgc atcgtgttcg atcgaactag taattatatg attctttata ttttgagatt
     7141 ttctaatact tttgcagatt cctttcaaag ctaaattatc agactcagtt ccaccagatg
     7201 taaaaaaaat ttcgcttggt ttagcaccta tgagcgatgc aactcttctt ctagcaattt
     7261 gaactgcgat ctttgattta attccgtatg tatggattga agaaggatta ccatataaat
     7321 ttcctagata tggtatcatt tcattaatta tttcctggag tactggagtt gatgcagcat
     7381 gatctagata aattaaatgg ttattggatt tatttttaat caagttaatc attgatcaca
     7441 attaacttgc cagagcgata tccgtataaa tctaaagatg catatttgaa accaatattt
     7501 tttaattcta gatctagctg attaagtttt attttattaa acattttttc catttcgtct
     7561 cttccaattt ctattcttgc tatctcacca tggtctctta ctcgaacatg actaatatca
     7621 aacaactcct taactttttc ttctgctatt tctatttttt ttaatttttc ttgagtgata
     7681 ttattgccat atggaattct agatgctaaa catgaattag agggcttatt tgcaacactt
     7741 atattaaatc ttttagcaat ttctctgatt tctttttttc caattctttt ttctaacaaa
     7801 ggacttttaa tcttatattc tctcatcgct ttcaaaccag gacgatagtc attcatatcg
     7861 tccaaatgag taccatcaac aacgtccgtt acttggtatt ccttctcata tcttttgata
     7921 ttttccccca aactttttct acagtaataa catctgttaa tatcattttt tacaaaattc
     7981 tcatcttcaa gctcgttata gtggattatt atgtgttgta ttcctatttc attggctact
     8041 ctttttgcat cttctagttc taataatgca agagttttgt aatttgcagt aatagcaatt
     8101 gctttctcac ccagcgtttt ttttgcagct aaagccacta cggcactatc tactcctcct
     8161 gataatgcta tgaatacatt tccttttgta ttctttttaa accaatcttt caattgtaaa
     8221 aaactttttt cactattcat aactattccc cttaaatttt ttaaatatct gatacataac
     8281 taattcttga attgctttta aggaactatc ttcaatatca gatatcttct tcaaatcatc
     8341 aaattctggt ttgatattga ttatattatt tgatgaatct cttgttattt ttactttgat
     8401 atcatactta gaatgattaa cggttacctg tataattatt atgttcctct ctagagttat
     8461 tctattaatt tcatttattc ttattcctga agtaccagtt tctttaaata gtctttcaat
     8521 gagagaatca ctcttattta tatcagaaag cactcttatt atgtttacgg gtcgattttt
     8581 tttggttatt cctggaataa ttgttacatc tcttgctcct tcttccatca aaacttcgat
     8641 taaattacca ataatctctc cgtcaacgtc atctatattt gtttcaatca aaaacagttt
     8701 ttcgttggtt aaaggagagt caactggatc cttccccagg actatcctga ggacgtttgg
     8761 tctgtctttt agtttcctat gaccggctcc cagacctatt ttttctggaa taataggagg
     8821 ataatatcta atacattcag aagatagatt aactaacatt gctgctcctg taggagtggt
     8881 taattctcct tgatcattcc cagttaatac aaacgacttg cctttaaaaa tttctagaat
     8941 tgcattggta ggatttggta tagttccgtg ggagaaatta gttaaaccat tacctataga
     9001 tatataagta gatactattt tggaatcaaa aaccttcaaa tcttctaagg caactgcaca
     9061 tccgattaaa tcagcaaaag tatcaatact ggaagcttca tggagataaa cttgatttac
     9121 aggttctccg tgaatttttg cttctgcatc tatcaaagtc tttattgaat ctacaataaa
     9181 agactttgga cgaggtttta aatctaagta ttctagggat ttggatatat tgtttaatat
     9241 ttcaattcct tttctttcct gaatttcctc tctatattca aaatagaatc tggttgcggt
     9301 aaatcccttc gaattaactt tctcgaacct gacatctttt attttgctcc ctgacataat
     9361 gttttcacag gtgtatattg aatcaattac cttttgttta tctgctccta gatctattaa
     9421 tgcagataac atcatatctc ctgataaacc tgccagctgt gcatcaatga tagaaatttt
     9481 agacaattat atacgttata ataataatta aacacaaatt aaaattactt gttttatcac
     9541 gatgtttatc aatgaactcc tttccaattc tgaaaacatg caattatact ttttaaattt
     9601 gagtaaactt ataattatcc agaaataatt ccagtctatg acaattacaa tcgtgggatc
     9661 tggaagagta ggtgcatctg tggcgttaaa ttgtggatta agagaactag atgatattat
     9721 acttttggat gtaatcgagg gtcttccaca aggcgaagca atggatatta accaccaatt
     9781 atctgaactc ggaatagatt ctaaagtaaa aggatcaaac agttatgagg atatgaagaa
     9841 ttcggatttt gttgttctag tagcaggtct gggaagaaaa ccaggaatga cacgaatgga
     9901 cttgttaaat aaaaacgcga gtattgtaaa agatgttgcc actaaaattt caacatatgc
     9961 aaataatgca acactagttg tcgttactaa tccacttgat cccatgacat atttcgcttt
    10021 aaaaactacc agattcgata aaaacaaggt tattggaatg ggagggatgc tcgatttatc
    10081 aagattcaaa agttttattc atgatgctag cggttgctca agggcatcta tccaagcgat
    10141 ggtgataagc gaacatggcg agaatatgtt acctttaatc cgattttctt caattggtgg
    10201 aatcccaata aatgaatttt tatctaaaga acaatctcaa gaaatatttg aaaaaacaaa
    10261 aaaagttgct gccgaagtta ttgcattaaa aggcgctacg gtctacgccc ctggaaatgc
    10321 tgttgcaaca atgattgaaa gtatgaagaa aaataaaaaa gaagtaattc ctgtttctgc
    10381 ctatctcgaa ggagaatacg ggatttctga tttgtgtata ggtgttccag tggtattggg
    10441 atctaatggt atagaaaaga ttattgaact agagttaaat gaatttgaaa gaaaaatttt
    10501 tgatttagga gttacaagtg taagagaagc aattaatttg tatactcaga aataaaacaa
    10561 tggaatttga agaattatta actcagtaca ataagggaaa tatatctatt aatgaactta
    10621 aacgaagcat tacctcttct tatatttaca atgtaggaaa aaatattgca aaattagata
    10681 ttaatagaag ttatcgaaaa ggaattccag aagtgatata tgcagcaaat aaagattatc
    10741 atgatattga aaaaataatt ttaaacatgt tagaaaaaaa taatacaata gtagtaagca
    10801 aaatccaaaa ggaacatata aaaaaattaa taacctttgt aaaaaagaag aatttttatg
    10861 ttgaaattgg tagaagtagt tctactattt tcttatcaaa aaattctaaa tttaaatacc
    10921 attattataa aattggaatt atttcaggtg gcacatctga tatcggtata gcagaagaat
    10981 caagattaat ggctaaagca atgaattgtg acgcgattat atcatatgac attggtattg
    11041 caggaattca tcgagtattt ccaactttag acaaaatgat atcagaaaat gtacaagcca
    11101 tagtagtagt tgctggtatg gaaggtgctt tagcttcttt ggtctcctca tgcgtaaatg
    11161 tgcctgttat cggagttcct acttcggttg gttatggatt cggttctaat ggtattgctg
    11221 ctctatcttc aatgcttcaa agctgcgcac tcggattaac tgtagtaaat atagataatg
    11281 gaataggagc aggctcattt gctgccttga tcgccaatag aatgagtaat atgaacaaaa
    11341 tcaaataata taaaatgatt aatagaacgt ttataagtgc ttgtaaaatt ctgcatttat
    11401 tatcaaaaaa attggagaaa gactgtaatg aattatatac atagtttaaa gaggattaga
    11461 gaaagaaaaa ctaactatca taaacgcgca tcattattat taagtaaaaa gaagtttatt
    11521 actataaaag ttactaatca aaatattcta acacaagtat tagaaccttc tattactggt
    11581 gataaggtaa tcacatctgc tcatagcact aatttgcaaa aacttggttg gaaaggatct
    11641 ttaaattctc ttcctgcctg ttacttaaca ggcttattac taggaaaaaa agcggttttt
    11701 aagggaattg ataacgctat actttatacg ggcaaagatt cctttactga aaggatttct
    11761 gcatgtttaa aaggcataat tgacggtgga atggagatac catcatctga agacgctttt
    11821 ccaagcactg aacgaatttc tggagagcat atatctactt atgcatcagc tttaaagtct
    11881 gattcagaaa gatataataa aagattttca agccttataa aaaatggttt aaatcctgag
    11941 aattataaaa ctcattttga ggagtccaag gaaaaaataa tgaagctaga aattcaaaat
    12001 ttcataaaaa atgaaaacaa taaagtgcct taccctaata aaaataaaat agagttagta
    12061 gatcaaaccc agggagagga gtctaaataa atgagtcatc atcaatttcg tagagaagaa
    12121 aaacaagtcg aatggaaacc taggacaaca ttaggtatgc ttgttgcagg tggcaagata
    12181 tcgtctatgg aacaagtttt tgaaaatgga atgcgaatcc aagaatctga aattgtaaaa
    12241 caacttcttc cggacatgaa aagtcaagta gtgcatgtcg gaattgttca aaaacaaact
    12301 gacgctggtg aaatgactag gtttgctgca ttggttgcag taggaaatga gggaggttgg
    12361 tttggaatag gtacaggcaa ggccgcacaa atgcgaaatg ctattgacaa agctactacc
    12421 gcatcttatc taaatgttat tccaattaaa ctcggatgtg gtagttggga gtgtaggtgt
    12481 aatcaattac attcgatccc attccaggta tctggaaagg ggggaagtgt gacacttgag
    12541 atattacctg gacctagagg attaggcttg gtagcgggag aagatataaa aaaactatta
    12601 aaattggctg gaataaaaga tgcttggaca aaatcctatg gctctacaaa tactatggct
    12661 tcgaccacaa aagctatatt caatgcttta agaagtacct atacagcagg ttaatgataa
    12721 gatatggtct atttagttat tagaattcgt ggcatagtta atattccaag ctgggcagaa
    12781 aaaactttag ataatctaaa cttgaggaaa aagtttaacg caacaatagt tacagaaaac
    12841 agtcaaactt taggaatgtt acgtaaagtg aaagacattg ttgcttggaa atctgttgat
    12901 gaatccatta ttaaagaact attggaaaaa agaggaagaa aaaaaggatt acatgtacta
    12961 actaattcta gtcttccttc gaattacaaa actatcgatg acttagctaa tgcaattcat
    13021 aaaaataata ttagtttatc cgaactagaa gacttgaaac cttggttttc attaagccct
    13081 cctaaaggcg ggttcaggag aaagacaaaa actcagtata cccagaaagg tacagtggga
    13141 gaaaacaacg aattggttga tctagtaagg agaatgatat aaatactaca aaattttatt
    13201 tatgcaagaa tatgatgatg atgatgatga tttcgctaat cttttctatt cataagcaaa
    13261 aattgagggg tcttggttat ggctactaga ttcagaaaaa gtaggaggca acgtggcaca
    13321 cgatattgtg gttggggaca aattggtcaa catagacagt caggctccag aggaggagtt
    13381 ggacaagctg gtaagcataa acatttctgg atacgtactg ttattgaaga gccagatcat
    13441 tttggacatc ctcaacttaa atcacatcac aaaattgatg tgaaaaaatg ggcaaatttg
    13501 agagacttgg atattttata cttaaaacat agtaacaaca aacttaatga aaatgagact
    13561 ttagatttaa ctgctctggg aattaataag ttgttaggag caggtagcgt tacaactaaa
    13621 atgactgtta aggtagaaca aatttctcga cgtgcagttg agaaaattaa agcaattgga
    13681 ggaaatgtaa tttccaatga acacagttca gaatgaaggt tttattagaa aatcaataaa
    13741 aacagtatcg agatatattc ctcaggtaga aaaaccaaag aagaaaatat ctttaaccga
    13801 aaaatttgtt tggtgtggta ttgcggtttt tgcgtacttg gttatgggtc aggttcctct
    13861 gtatggtgtt actgattcac cccaattcga ttttcttgca tttgcacgtg taatttttgc
    13921 cgcacaacag ggaaccatac ttgaacttgg aataggtcct attgtaactg caggattact
    13981 tatgcaattg cttaaggggt cagatatttt aaaattaaat ttcaaaaatc ctgatgatag
    14041 agcccttttt acttcagcaa ctaaaattgt aacaattatt gttattattg cagagggagg
    14101 tctatatgga gcgagtgtct atggaccttt agtagcctca cctagtttta tacctatttt
    14161 aatcgcacaa ttgatattta ctagtcttat tgtaatgtta ttagacgaat taattcaaaa
    14221 aggatgggga ttaggatcgg gaataagctt gtttatcatg gctggtgtgg ctcaaacaat
    14281 cttatggagt gtatttagcc ctgttgctgg cccagatggt ccagtgggaa ttatcccttt
    14341 tgtaatcgat tctgcagcaa ctggagatct ttctgatact ttctttagaa gtggtcaatt
    14401 acctagtatt tttaccttta ttttgacggg aggtgttttg gcacttcttg tatatactca
    14461 gggaattcat attgatattc cgattgtttc aacaaagtat cgaggcttta cagcagttta
    14521 tccaattaaa cttctttatg tgtctaatat tcctgttatt ttatctactg ctttgatcgc
    14581 caatgcaatg tttatgggtc aaatgttatg ggcaaattat aatccacaaa atgataatcc
    14641 attttttaac tggatcgctc agtttgatcc aggacaacaa caaactccta ctggtggatt
    14701 attatactat atgagtaccc caagaggact cgatatagtt gctcaagatc cgatacgcgc
    14761 tttaacgaat atcatctttg taacagctat tgttactgta tttggtaggc tatgggtaga
    14821 acttggggga ctttctgcaa aagcggctgc gaaaaatctc ttggatgcag atgttcaaat
    14881 accaggtttt agaagatcac agaattctgt tcaaaatgtc ttggataaat atattccctc
    14941 tgtaactata cttggtggta ttgcaattgg actattggca tcggtttctg atgtacttgg
    15001 actttttggt ggtggaatag gattgttgtt aatggtagat attctagtaa actactataa
    15061 cttgttgatc agagaaaagg tagatacgac aatgccaaaa ttggcggctt taattggaag
    15121 aaaagactaa acgtataatt gtagttggta taccaggggt cggaaaaact actgttgttt
    15181 ctagtgttaa aaatacacta gaacaaactg gtatcaaaac tactctagca gaatttggaa
    15241 agataatgtt agaagaggca aaaaaaatga aaattaataa tagagatcaa atacgacgcc
    15301 tttcaattga gaaccaaaaa aaattacaga accttgctgc cgaaaaaatc tcacaaatat
    15361 attctgacgt tgtaattatt gatactcatt tatttataaa cactaaagaa ggatactatc
    15421 cagggttgcc agcggaccta ttaaaccatc tgaaaccaac tcatctaata ttaataattg
    15481 caaatccaga ggaaatttat catagaaggc aaaatgactc tagtcgtgtt agagatatta
    15541 tatcaatgga ttctataaaa aatgaaattg acgtgtctaa gatgctgatt tcttgctgtt
    15601 ccataatttc tggagcgcct ttcaaaattc ttgctaatca tgagggctta gtgacagatt
    15661 gtactcaaaa aatggtttca ttaatattgg acacatgaaa aaagacaaat aatatgtatt
    15721 taagtgatct tattttacag attcctggac ttactccagc atatactaaa catccaatct
    15781 ttccagattt attttctgct atgattgtag ctgctctaat atcaattggt ctaaactttg
    15841 cgttcgcgat cctgagaaaa aagactactg atattgatag actttccaaa gtgatgaaag
    15901 aaacaaatga atggagaaaa gaatatactg atgctattag aaaacaagat aaagttagaa
    15961 ttagcgagtt aaagaaaaag caagcgtatg ttaataaaat gagtttagaa atgcaacaac
    16021 aacaaatgcg acctatgatg ctttatatga taccttcatt tatgctctgg attcttgtat
    16081 tcccaactat ttttggacaa actgtcgccc tttcaccact ccatattcct tatctaatgt
    16141 gtacagatga aaacgtgaaa aaagatagtg tcctcgatgc aaatggtcaa cccacaggtg
    16201 tctgcaatat ccctggagaa gtttttttat ggggttggtt tctcattact tcgctgggaa
    16261 ctagcggcct tatcgccaaa atcaccaaaa catccatgcc aagtatgaca taaattgctt
    16321 acttctaatt acaaaaataa aatattaaat agtattgtaa tatctggttg gccagcagta
    16381 ggtaaaacaa ctattgctac agaattagcg aaagaatttg ggtacaaaca ttacaatggt
    16441 ggggatgtgt taaaaatgtt ggctgccgaa aaagggtata tagtatccgg aaatgattgg
    16501 tgggatacag aggaagcaaa aaatttcatg gaagaacgag aatctaactc ttatttcgat
    16561 atcgaagtag ataggaagtt ggtagaaata gtaagaatgg ggaaagttgt agtgacgagt
    16621 tatacattgc catggttagt tccagaatcc attaaaatct ggttaaaagg ttctaggagt
    16681 aaccgaataa agaggatggc aaagagggat agcttgaacc ttaaacacgc cagaaatgtt
    16741 atccaacaac gcgatgaaaa taatgtacgt atctataata atttatatgg ctatgagttt
    16801 ggaaaagatc tttctgtttt tgattttgtt ttagatactg atttattaaa tcttgattca
    16861 cttcttttaa tatctaaaac aatagtaaaa aatttacggg tttagttcaa attctttgat
    16921 cataatgaaa gattaaaatt attaatattc tttatacaat atttttagca atggatttaa
    16981 aacgtaataa aaaactcatt cagtttgata atttaattag agtatcagaa agtgtaacag
    17041 atgatagata tggttatttt ccatatgatc gacccatcca tatattgttt gattatggtt
    17101 taatattgat cgataagcca tcaggaccga ctagtcatga agttgcatcc tgggtcaaaa
    17161 ggatattgaa tatagataaa tcaggtcata gcgggacatt agatcccgga gcgacaggtt
    17221 tattgccaat gggattagga gagggtacca aagcactctc aattttattg ttaggaccca
    17281 aggaatacta ttctattgct cgaattcact cgaactttga tcaaaagatt cttgaaaacg
    17341 tattattgga atttcatggt gacatttacc aacgtccacc acagcgatca tcagtaaaga
    17401 gaatgacacg aatacgtcga atccacgaat tagaatacgt agaagaagat gacaaactta
    17461 ttctgcttcg aatattgtgt caagctggaa catatataag aaaattaatt tatgatattg
    17521 gggaagttat gggaacaggc gctactatgg ttgaacttcg tcgtactcgt gtcagtcatt
    17581 tctttgaaag agatggtcta atcaaacttc atgatttgta tagggccttt tatttatata
    17641 aagagaaaaa tgatgaagaa agacttagga atatcgtaaa ccctattgag atatgcttca
    17701 aggaaatccc ctctgttgtt attagagata cagcagttga tgctctttgt cacggtgcac
    17761 aattagctat tcctggaatt tctgaacttt caccatcgat tcaaaaggat gatttggtag
    17821 cgatatacac tttaaaagga gaaatagtag gacttgccca atctcttatg aatataaatg
    17881 aaattgtttc taataaaaat ggtatttctt ttactatgaa gcgactcatt atgaaaccta
    17941 atacttatcc aaaaggttgg cgttcatgat ttataaatat tgatttctga agtaatttga
    18001 gagaatgtaa gtaaatatcc tataaaaatg aattaaatta gatagtttct cctatacctg
    18061 gagtctttct atctaaatta gcaatctttt ttgcaagatt ttccatcgtg cctggtatat
    18121 gacatttaca catacaatca gaacattcta tatggttatt tcttttacaa tattgagaaa
    18181 tttctgcgct tttcatttac aatagaaaaa tttcctaata ctatattttt attttatcat
    18241 gatttaaata caaacagaat taatattata aaacgttatc caatagatta tattttccac
    18301 tttattcctt tattgtaatt taaaaagact tactgccggg gtcgcctagc ctggtagggc
    18361 gcaagcctgg aattgtcaag aaagcttgtg accgaaaggt ctcgagggtt cgaatccctc
    18421 tcccggcgct tatcctgggg attcatacga taatatttta agaataaaca aaaattatta
    18481 tcttcgtaca taattgcaaa taactaataa ttctcatcag gattttgaaa ataataataa
    18541 tgaatttaat aatggacagc aaagaattga gtcattaacg aaactacagt gtccatctat
    18601 taaaacggca ttaaaaaact agcattagag aattaagaaa attacaattt aacaataaca
    18661 tttaaaataa taaaattagg tttgatattg atttttactt ataattcctc aattagaaaa
    18721 atcagttatt tctcttccca attttttttc caaattctga attctccttg catacatttt
    18781 gagtctatca tcttcatgct tgtttttggc aattgtaaat cctagccagc atttgttaag
    18841 agctcagtac gtctgatttt cataataaaa agatggtaat aagtcataat atttctgact
    18901 ccgtttctca tgcgcaaccg ggttccatcc cgtttgtttt acccatttat cgtaattaaa
    18961 tcggtatttg tgatatctca tattacaatt aatagttaca gcttctttta aggtttgttt
    19021 tatttttaat actatctagc tgtatatgtg ttcatcatag aataaattat tttataaact
    19081 ttactaaatt tatttttgaa ttaacaatat tcctttatca ggatcattaa aataatggat
    19141 attcatgata atgatgactt gataattata ttatgtaatt aattttacaa aattaatatc
    19201 taatactgta taatttcaaa taagttgatc attcttttct tgagctccaa taagtagtgc
    19261 aaagataaag tccaatattt tacgtactca aacatatatc aattaatatc tcgaattaaa
    19321 tctggagtaa ctttatagag gagaagattt agtataattg cttacgatta ctatttcaaa
    19381 aattatagtc ttatacagat tctattactt actagaatca ccctttaata attattatat
    19441 ttgagtttgt agttttttag ttttatctta atagtataaa taaaattgtc aatattgtta
    19501 tatgaataac aaattgttac ctattaattc tgatgataat aaagataaaa caaataaagc
    19561 caaaatttat aactataaat ctacagaaat tgtcaataaa gaaaaaataa aaaaattaaa
    19621 aattcatatt gacggagtta acaaaatcag taataaaaag aatattattc ataaaaaaat
    19681 ccaactttgg actaatgata aaggtaatga gtatgctata tgtttattat ttgattcaga
    19741 tgtaaaaata gataatgccc aatttatcgc gtcaacaaat cctaacgaga aatatataca
    19801 tccaattgca gaaacggata atattaaaga cctacaacat ggatgggatt atacattgag
    19861 agaagtgata gaaaattata aaaaaaatta ctatcgcaaa atcagcggag aggacattca
    19921 atccattaaa ctaattcctt tttataaatg caaatattgt aataaagaac tttctagtga
    19981 agatgaaaaa aaggatcatg aatttttatg gcatatatag aaaataaaat ttaagatgag
    20041 tcactaatta gaatattctc tttatatgat ttgcagctct gtttacaaaa aaccctaacc
    20101 aaaatagata agaataaaga tcatggattt acctgttatt ataaaaataa ttgaaaaaca
    20161 acagaaataa taatttcatg ttagaacaat tatactcatt ttcgatgatc tatagaatgt
    20221 aattaaaata ttttaccaga aagaagatag agaatacaat aagtttccaa ctgttctttc
    20281 tataatcata cttgatacaa tgatgtttgt cttgagaacg ggatttcaaa ggtgtttccc
    20341 aaagaatatg gttctggttc tacatctcat agaagatgtc aggaatagtg ccatcagctt
    20401 tatttgaaaa attatagact agattcttat ctatgataac aaaagaagga ataaagtgga
    20461 actaacagtg gcgtgacagc atatcaataa agtcgccttt aggtaggtga tgccgggtct
    20521 taaacccgca gacataatca aattataatc caaaagacat attttgacag ataaaaactg
    20581 gattacaccg tctgctaata taacctctgt aaatactcat gaggttattg ttgcaacaga
    20641 tacagttgat agtatattaa gtgatcccca acacccacca tcatcaaaaa taagttattt
    20701 acatgatata agaagaagga tgaaaatctg ttagtattgt gcaactagca agtagtataa
    20761 ttgattaaag gagaataata ttgggatagc ttcctaagat gaattgacct tcatttacaa
    20821 acggaatttt attttcaatt gatgttcaat acaaataaat gaattatctt atccaattgt
    20881 ctaccggttt ttttataatt tgccttctct atctcaaaat gacacattta cttgcttata
    20941 atattatttt taaacttttt tataagtttt gaaatgttga cggatcacaa aagttatact
    21001 attcatgaat taattattaa atcataattt ttctttaata gtatttttta taacatttag
    21061 atgtagattt tgtaagtaat tcctaagaaa gaatagtaga aaataatcat ggtaccagat
    21121 aaacaataaa actagcaccg acaattccaa taataatgag tgtagttaaa ataattccct
    21181 ttttcgtaaa tttattttta atttcttttt tgttattcaa actaattatg gtaaaaggtt
    21241 tttttattta tttttttata ttaaaacaaa gcatagctct tttagtattt aggttttttt
    21301 cgcagtcttc tctctaattt cgctatcttt ttgctacgag atctcttttc actatgagca
    21361 tgcgtttttt taaaatcttt cttcatcatt ttttattttt tatttcttta ataaatactt
    21421 acaattattc ttaaccacta ttaaaaattg ataatgaata tgaattgata tcgttgaatt
    21481 atagttttca tagcaaatat gaggtatatg tatctgagct gtctaaactt tttataatac
    21541 agttaataat ttaggctgtg gagaatgtat gtattttagg agctggtagt actaaatatg
    21601 gtaagcttaa tgaaagtatt atagaaatgg cccttagtgc atcagttgat gcaattgaat
    21661 ctgcagggat atcacctaat gatattcaag ctggatatat ttcaaatgta tttggtgtag
    21721 cagataagca agtacatatc gctccagtta ttatgagcaa tttaggaatt tctcatgttc
    21781 caggtttgac tattgaatca gcatgtggat caggttccgt gatgtttagg gaagcatttg
    21841 caaatattcg tgctggattt tacaactgtg tgttggcttt aggtgtggaa aaaataactc
    21901 atactggaac aactcagagc acaactctat tttcatattg ctcagatttc ttttatgaag
    21961 gaggaaatgg tgcctcattc cctggtttat ttgcatctat tgcacgagca tatatgacga
    22021 agtataaagc tgacgaagaa gatttggctc atgttgctgt aaaaaaccac gaaaatggat
    22081 tactaaatcc taaggctcat gtgaggaaga agataactgt tgaagatgtg cttaagtcac
    22141 ctgtagttgc ctctccatta aaactatatg attgttgtcc tttttcagat ggtgcctcag
    22201 cagtaattct ttgcagtgaa gattttgcaa aaaaaagtgg aaaaccatac attcaagttg
    22261 ttggatcggg aagaggaggc tctccagcgg cagttcaagg cagagaagat atttcaacta
    22321 ttcctggtac agttattgct gctaatcaag catataaaat ggctgggata ac
//
LOCUS       DASVEV010000059        22858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_15464, whole genome shotgun sequence.
ACCESSION   DASVEV010000059 DASVEV010000000
VERSION     DASVEV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 22858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22858
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15464"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VF222_03090"
     CDS             complement(<1..605)
                     /locus_tag="VF222_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7141445.1"
                     /translation="MDFKEIILLSLDAIKERKTKSLLTIVMVMVGSSLMIAVSGMGAG
                     FSEFFNKQTSNLAGNIMFINPAPQTQSGGTGPGGGNNAAPPPVAKITLNSAVEKRLSS
                     LPLVKEVIPSYQSQVTIESKGESKDFAIFSLNPQKLTIIAPTLKYEEGSVVRFNDPSA
                     MIVSSDVAFPPGETVPYLSLGQTVQVSYTFVDPITGNRESET"
     gene            complement(684..2834)
                     /locus_tag="VF222_03095"
     CDS             complement(684..2834)
                     /locus_tag="VF222_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141446.1"
                     /translation="MTLTFFIIFSSLFLLFVLVIFINSNSLILSNGYGQREPLDDRSP
                     TFLEAYWTNKPTTTLSQSQVPDNPIKVEVGPGEGPSTLAVVLVNTGRSEITGITGDLT
                     LPEGFKAIRGENDVKSENISVASYNSIAKPGESFPLYFTFGVLNDTKVGPYYGAMKIT
                     YSKVLETGQIVSNIAVPFRIPGKVILDVILQKQTLITNTPNNLPILLYNKGSANANGV
                     IVTITNISGGTTTIGNKGLQNNANSNIDNSILYNNSEANNNNNNNLGINYTIPINSTN
                     ATENILTKQMSQRELHVRPIDLQATTFNVGRILANNTALIIPLIYPDYSSGGTIQNMK
                     LKIDYNDPYGNKKNLETSVGLIISPNPPESVLSIDEAIKVLDTNNTDKNNNNNNNNTV
                     DNITSNTTKKDNLMLRAGMIEGAKITVFNNGNEELEDVVFSLKSESDSVKILGDTRWT
                     LSSIEPFSKKELLTQIFSSEEVISKPISFTLDAEYISGGKSKKDSLSIGAYIQGQIKI
                     RTYEVSVENIGGVLNLVGNLLNEGNTVALFTTIEIINNTNNELSSKLTSNFSQTGDRI
                     KQLPLQSLSSPQYLGDLTENSPLPFSIPIAINSDTENGKYRTNLKIIYKDDLRTEHDL
                     VLNETVYYIPVEGDQTNKSDKSSSSIFGFTISNFVTTIIVIFLLFGLIVIAIIFILFT
                     VKKRKNKKSKLYESLKNQDNNMKDFLGDKKDERV"
     gene            complement(2928..3602)
                     /locus_tag="VF222_03100"
     CDS             complement(2928..3602)
                     /locus_tag="VF222_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7141447.1"
                     /translation="MELKNITKIFESPAGESVVLEDINFKIKRGELISIVGPSGSGKS
                     TLLNIIGVLDKPTSGEVIINGFNILSLNESEIAQMRNNLIGFIFQSFNLINRTSVQKN
                     IEIPAIIMGMDKSVRHYRSQKLLELLGIKDKAEYKPMNLSGGQQQRVAIARALINDPT
                     IILADEPTGNLDTKTGEEVFDLLKMLTRKYKKTIILVTHNQELALETDKTIFIRDGKI
                     EKEILN"
     gene            complement(3915..4541)
                     /locus_tag="VF222_03105"
     CDS             complement(3915..4541)
                     /locus_tag="VF222_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141448.1"
                     /translation="MIIGICALFAISILTPNNTFQILVFHEIKASVLDQNASSNIQQN
                     QPLQQQQQNQPLQQQQQNQPLDNNLIIGSFGNDRLTGTAIDDIIVALSGSDTVHGLTG
                     NDKIQGNEETDQLYGDEGDDIVQGGSGSDLLHGEEDDDVIAGGVDDDYLIGGNGNDKL
                     YGDVGDDILSGLKGADYFDCGDGVDVIIDFTLNESDDTAGNCEEIQNN"
     gene            5603..6202
                     /locus_tag="VF222_03110"
     CDS             5603..6202
                     /locus_tag="VF222_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7141449.1"
                     /translation="MSTDNDNLDSDGNEIISPDGSVSPELEDEPENIKSKESKDYSNS
                     KDSIPSFKKILVADDGKDESDAALLYAVSLSNSTGAELLILRILEDVEKFEHVTVEGS
                     HESSKMDNYDFHRHIKGDVIDAMEEKIKKCEAAGCKNKISYKFRAGKADDEIVKEIKE
                     NNFDLLILTTSHIDSWIRSLFSDARKIISNISIPVLIIQ"
     gene            6257..6550
                     /locus_tag="VF222_03115"
     CDS             6257..6550
                     /locus_tag="VF222_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141450.1"
                     /translation="MSLNNNLRGLLENIIDESIIIMPALMTLFRNTIPPEFGIQRLED
                     YAFGYVQGLILGQFISSYRAIFAQNLQPEISAEAGRILLRRSRDIRDAIFKAG"
     gene            7445..7630
                     /locus_tag="VF222_03120"
     CDS             7445..7630
                     /locus_tag="VF222_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141451.1"
                     /translation="MNDEIAIINFITTIENINSIPVPESNQEYMCEFIGKRVLITVRK
                     RKVKGPTIEGEKPEDNE"
     gene            7743..8708
                     /locus_tag="VF222_03125"
     CDS             7743..8708
                     /locus_tag="VF222_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7141452.1"
                     /translation="MNLSSWYKKSAPLFTAKESKSILHDFFVYGKEGVTINPYNGCQH
                     RCGYCYATYEWSPEFYDHIYAKINATDLLYKQIISWKGKIITPVMIASATDAYQPAEL
                     KYSLTRKCVEVLQEFEIPYYVFTKSSLIERDLELHKRYSNNCFVVWSITTNNENIKRV
                     IEPGTPTSKSVFRVIENFYKSGIKCVINIDPILPLITDSTENIASIINSANDIGVEYV
                     SGAILRLRYDIWERMKYILQTLQIDNSIKLYEKIFKFKDPINFKKNLNTEREYSVSIM
                     NYLENELQKRGMKFGYPKLNIKKISSSSSFLKNKKNIVSLEDFMK"
     gene            8824..9393
                     /locus_tag="VF222_03130"
     CDS             8824..9393
                     /locus_tag="VF222_03130"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEX7141453.1"
                     /translation="MAASNYIIFLSLLVLSLNNSENIFNDAFGFFHLGNSQDDFKPYE
                     NTKLKLSIQYPSSWQKEERLNDFVTFIAPIVDSSQYKSPAGLGVSSLSVSNVSLATIT
                     NVHLKNMTNNLKDFQLVESSDTVLADNRPAKKIIFTATDDGEKKQALQLITKNNDKVY
                     LITYKANVDKYEQYFPIIQKMLNSLVFLK"
     gene            complement(9762..10373)
                     /locus_tag="VF222_03135"
     CDS             complement(9762..10373)
                     /locus_tag="VF222_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141454.1"
                     /translation="MQIIKKYRIIAILTLSIVAGLYNNALSYAQESNQTRVEEEVEEY
                     ATEARHSLEMNKYNKTNNVRDSVTILLQDTIIPGTDFLHLYDSTPSHIVNGHIALKAP
                     CGADSTSPIQVLIGSAPNMTAATLENITALSTPGEQCLYHVDLIPTGNVTTITDIALS
                     NPTEDEIEFPPTSSVVIGINEVMEGENTHRENTENATESTHTQ"
     gene            complement(11633..12400)
                     /locus_tag="VF222_03140"
     CDS             complement(11633..12400)
                     /locus_tag="VF222_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7141455.1"
                     /translation="MKDNEFFLIGDMNAHQIKKKVNDDTVALLIFGSCENHGNHLPFG
                     SDFIFPLELAKRVAIKIKNLIIFPPIPYGVSTHHKKFYMTMSIGTNTLVSIIEDLLES
                     VYNNGVYKILIINGHDGNIAPIEIASRNTKDKYPNITIACLESWWELIGKISPDTFNV
                     WDGLGHGGEAETSAIMKVRPDLVNKNNIPEDTMPNLPKHLRIFWTMDELTKTGATGSP
                     KNASEKKGDVICRILEDMLTSFLMDMEKTRWKYGLYL"
     gene            13352..14932
                     /locus_tag="VF222_03145"
     CDS             13352..14932
                     /locus_tag="VF222_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7141456.1"
                     /translation="MVTLITINYSMEDSLATEDFTNKTHTLSELFKNVEKSVVQISSE
                     DETTELLGSRLGSGFVYDTNGHVVTNNHVTSGGKDLHVTFSDGTIYTGKVIGSDPHSD
                     LAILLIDDVPKEKLFPLTLGNSSNLIVGETVAAVGNPFGLSGSLTEGIISGLGRLLPS
                     TPENIFGNDPRVATFSIPDIIQTDAAINPGNSGGPLLNLNGEVIGINSAIFSNTGVYA
                     GVGFAIPSNTLKKVIPELLKNGSYPHPWLGITGVDVTPDIAKKMNLTEARGFLVIDVN
                     SNGPADKAGIRGGDKIDTIDGREVELGGDIILAIDGNPVRKIDDILSYLEREKSVGDK
                     ISLTIIRDGIVQEKTTVLQARPDSQTLISSSQHPSLGITGIDVMPDIAKKMNLTEARG
                     FLVIDINSNGPADKAGIRGGYIVEIINNTQYRLGGDVIIGINNQTINSVSDITDYLKN
                     TKEGDIVKLKILRDNKMINVPVKLEIITMPLDQPDEIPPFNDNPKDFGDDQGKFTEQL
                     KKECLLNFNESICNFLFR"
     gene            15004..16056
                     /locus_tag="VF222_03150"
     CDS             15004..16056
                     /locus_tag="VF222_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141457.1"
                     /translation="MKQFLVIVISLIILLSFYSLILPNVYGNGIRNNFGIDYLPPLNL
                     EDTPLSLHVEFKPSMTKSNINEKRSLIFQLIDFDKNITHVPDFYNITILKSELYDGKV
                     IENIFLNGSFISKNGTLILEFDPNESRNENRNNNHYIIDRGWDEQRQQHQMNADKNGI
                     ILIDRPKLDSGMYRLKVNVALSDYSDLNKNINLKFDSYLSLGNILDFVIDNPDKKITI
                     LSFNDKIVNYGYDQQSRKISFEIPFKYNMTRIEEGFIYIHMEIKIPDTFNELFNVKEY
                     WSTINNIDHEKISSASVSVDRYSNSSNVMVHFVLDSNSLFKLTKEREEYPTEERSEMT
                     LRFSLQPKSTILNING"
     gene            complement(16229..16507)
                     /locus_tag="VF222_03155"
     CDS             complement(16229..16507)
                     /locus_tag="VF222_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141458.1"
                     /translation="MNRTNSLIILASLTLGAFLVFNNAPMIDVSASGDRSEHYYPPTQ
                     DYPPTQDYPPANGYTSDMQYNQDRYGGDSDQYESGYYEDHYGKPHKIK"
     gene            complement(16703..17698)
                     /locus_tag="VF222_03160"
     CDS             complement(16703..17698)
                     /locus_tag="VF222_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7141459.1"
                     /translation="MTVTEGKIIIHNFESSVLKSNPLKDPHNREIIVYVPHDYSQSRS
                     KGFPAAIGLSGFGGNGRTLFNIDPFSENVEQRMNRLICEKKCGSMILVIPDCFTKFGG
                     NQYINSTATGRYEDYIVKEIIPFIKNNYNISSFGVWGKSSGGYGSLVLGMRYPEIFKA
                     IACHSGDAAFEYCYLPDFPSAYASFKQAGGIRNWFNQFWNKPNKKEKSDFLALNILCM
                     AAHYSPDPASPLGVRIPFDLETGELNDDTWKMWKRLDPIKMISQYQNNLKKLSFLYLD
                     CGTKDEFNLHIGARILHQKLENMDIKHYYEAFDGGHSNTSFRYDTSFPMIYSSLS"
     gene            complement(17786..18499)
                     /locus_tag="VF222_03165"
     CDS             complement(17786..18499)
                     /locus_tag="VF222_03165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7141460.1"
                     /translation="MQAKEFNISTEDSRQILENLLESKTRLVIIHVDLVGFTKLCMNL
                     SANRLADIIRAFTQEMSILIASYGGHIIKFVGDAVLGFFLVRSSDKNFDKIPCINAVN
                     CALSMTTVIRHGINPILSQYDYPEIHARIGVDVGENVVVQYGWETSVLKHGTENIITK
                     KPIIDILGYTISIAVKMTSLAESDQIVIGQLVYDSVDNNLKERFKDLPINTEVWNYIS
                     SATGGIYRLYGSIRDSTYY"
     gene            complement(18844..19134)
                     /locus_tag="VF222_03170"
     CDS             complement(18844..19134)
                     /locus_tag="VF222_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141461.1"
                     /translation="MTSLNNDKKIHVIVHGNRQNYDKVKNMLVEKKLNEEKIAMASLE
                     KAGEIGEYVAMTWPPMAAREVIVSEIVGTNAQGEGMGAWGSIEQKELFRFPL"
     gene            complement(19210..19479)
                     /locus_tag="VF222_03175"
     CDS             complement(19210..19479)
                     /locus_tag="VF222_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141462.1"
                     /translation="MKKNRFHSLFLPLVVSGIIISTFGFFLIYLGQSIEHGEGFKIVG
                     MRFIVAGIATIGLGFFAQYYTKIRNSLLVFKYFVQSFKKNNRSYR"
     gene            19560..20492
                     /locus_tag="VF222_03180"
     CDS             19560..20492
                     /locus_tag="VF222_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX7141463.1"
                     /translation="MNIAVLGNNEFLNEFGKRGTLSDIVLYDRKLNDRIFTLIAPISF
                     PDKIQTLPQVLNMTDFVVLNITHLDKYLAEQIIAISSLDIRNGFLLHSYNIDEQRLNQ
                     MIKGTSIENFKKIENLEILKEEINNLHSKSKEGDLLMPIDHSFDVKGVGTVLLGVIKQ
                     GRIRVYDKLRILPSNKEVLIKSIQMHDDPVDESSSPSRVGLAVKGISPSEIERGDIIC
                     SGDTIKTADTEVIVTDFSKNKYYDGEITDTQTYLLSVGLQVKPLKILINNSKILLSLE
                     KPIVYYEKQKFLILKPDSKTTRIIATGSFPSVIR"
     gene            complement(20695..>22858)
                     /locus_tag="VF222_03185"
     CDS             complement(20695..>22858)
                     /locus_tag="VF222_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HEX7141464.1"
                     /translation="FWEGLDGTQILTHWMPLGYRAGLFLDQLSLSLKQLKKYASTNQI
                     LMLSGSGSTPPQPELVEVIKEWNKKVNGKKVTKPKSGYNMIMSSPLQYFNYLEKQNIK
                     FKVRKGEMYSGKFSAVYPDCTSTRMWLKQGMKEFENSILLLERLDALAWLINIHDENI
                     SDQLHKYWKKILFIAMHDVLPGTGVDEIYNEVREIFENERKSIDRSIFEYLRKLSLMT
                     NYDNDIFIFNPLSWLVTNWTEILLEFNEGEIKGISCLKNGDQVIEIEILDYSLYQDNS
                     IKKISIGCIISVPPLGYKTLEIVKSDTIDGETLIAHDTSFKNKEYEIEVNRENAIITV
                     FRSIKNNKKLYYCTGNEIILDEEIGDLYYHRENLKLFKSETGDGIKYGIFKPEKYEVI
                     HRKLKSHIKFKSKYFALRWPYRFTEKLKPMMYRHDFIDIEKEIIIYKNLNRIDFVTHI
                     FNKHPHSRLRVKFDTKGTAKSYWCGTQFGAVERKPDLYDQKDSSKWFEKPSGVFPSLE
                     WIDYSIKNDSNKTVAISLLHQGLPSHEIRNGSIYITLLRSIVLLSSDGIMGPCIPTPD
                     AAEVKPYTFKYSILPHENDWSEDLIYQHGSEINMPLIPIQIKKNNGRKNNIAENNGRN
                     NVKVLDDSKSFLEISESNVILSTMKLSDDKKGIILRVYETEGKKTITKIKLAAKIRKT
                     KLIDFMENEIQEIKKIENDTITIDIDPFKIITLKIILK"
BASE COUNT         7796 a         3433 c         3258 g         8371 t
ORIGIN      
        1 gtttcagact ctctatttcc tgttattgga tccacaaaag tataagatac ttgtacagtt
       61 tgtcccaatg ataaataagg tactgtttct ccaggtggga atgctacatc gctggaaacg
      121 atcattgcag aaggatcatt gaatctcaca actgagccct cttcatattt taaagtcgga
      181 gcaataatgg ttagtttttg cggatttaaa gaaaatatcg caaaatcttt tgactcaccc
      241 tttgattcaa tagttacttg agattgatat gaaggtataa cttcttttac taatggaaga
      301 gaactcaatc tcttttctac tgcggaattc aatgtaattt ttgcaacagg aggtggagct
      361 gcattattac caccaccagg acctgttcct ccactttgtg tttgtggtgc cggatttatg
      421 aacataatat tacccgccag attacttgtt tgcttattaa aaaattcgct aaagccagca
      481 cccattccac taacggcaat cataaggcta ctcccaacca ttaccattac aattgtcagt
      541 aaagattttg tttttctctc tttaatagca tccagcgata agagtattat ttctttaaaa
      601 tccatttcga atttctttac cctttatttc ttttactagt tggtaaaaca tcataataac
      661 ttttattttt tattaattgc ttttcagact ctttcatctt ttttgtcacc caaaaaatct
      721 ttcatattat tatcttgatt ctttaatgat tcatataatt ttgatttttt attttttctc
      781 ttttttaccg taaataaaat aaatattatt gcaataacga tcagaccgaa taaaagaaaa
      841 ataacaatta tagtagttac aaaattagat atagtaaaac caaaaatact actactactt
      901 ttgtcactct tgttagtttg atctccttct acaggtatgt aatataccgt ttcgtttaaa
      961 actaaatcat gttctgttcg caaatcatct ttataaatta tttttaaatt tgtccgatat
     1021 tttccatttt ctgtatctga gttgatagca atgggaatac taaaaggtaa tggtgaattc
     1081 tctgtgaggt ctcctagata ttgaggtgac gataaacttt gcaaaggtag ttgttttatc
     1141 ctatctccag tctgcgaaaa atttgatgta agtttagaag ataattcatt attagtatta
     1201 ttaataattt ctatagtagt aaaaagtgct actgtattcc cctcatttaa caaattccca
     1261 actaggttta gaaccccacc tatattttca acagatactt catatgttct tatttttatt
     1321 tgtccttgta tataagctcc tatagaaagt gaatcttttt tagactttcc tcctgaaata
     1381 tattcagcat ccaatgtaaa tgaaataggt ttacttatta cttcttctga tgaaaagatt
     1441 tgtgttaata attctttttt tgagaatggt tctatagaac tcaacgtcca tcttgtatct
     1501 ccaagaattt taacagagtc tgattcggat ttaagagaaa aaacaacatc ttctagctct
     1561 tcatttccat tattaaaaac agttatctta gcaccttcaa tcattccagc tcgtagcatg
     1621 agattatcct tttttgtagt attggaagtt atattatcaa cagtattatt gttattattg
     1681 ttattatttt tatcagtatt attagtatct aagaccttta tagcttcatc tatactcaag
     1741 acagattctg gaggattagg ggaaattatt aaccctacag aagtttctaa atttttttta
     1801 ttgccataag gatcattata atctatcttt aatttcatat tttggatagt tccaccagaa
     1861 gaataatcag ggtatattag tggaataatt aatgccgtat tatttgctaa aattctccct
     1921 acattaaatg tcgtagcttg aagatcaatt ggtctaacat gcaattctct ttgtgacatt
     1981 tgttttgtta aaatattttc agtagcatta gtagaattaa ttgggatagt atagttaata
     2041 ccaagattat tattattatt gttattagcc tcagaattat tgtaaagtat tgaattgtca
     2101 atattactat tagcattatt ttgaagacct ttgtttccta ttgttgtagt tcctccagat
     2161 atattggtaa ttgtgacaat taccccgttt gcattggcag atcccttgtt atacaataaa
     2221 attggtaaat tattgggtgt gttagtaatc aaggtttgtt tctgcaatat gacatctaaa
     2281 attactttgc cggggatacg gaaaggtact gcgatatttg acacaatttg tccggtttcg
     2341 agaacttttg aataagtgat tttcatagct ccataataag gacctacttt tgtgtcattc
     2401 aaaactccaa aagtaaaata aagagggaaa ctctctccag gttttgcaat tgaattgtag
     2461 cttgcaacag aaatgttttc agattttaca tcattttcac ctcttatagc tttgaagcct
     2521 tctggtagtg ttaaatcacc tgttattcca gtaatttctg atcttcccgt attaaccaat
     2581 actaccgcaa gggttgatgg accttctcca gggccaactt caacctttat gggattgtcc
     2641 ggtacttgag attgagataa tgttgttgtc ggtttatttg tccaatatgc ttcaagaaat
     2701 gttggacttc tatcatcaag aggttccctt tgtccatagc cattactaag gattaaagaa
     2761 ttggaattta taaaaattac caaaacaaat aacaaaaata gagaagaaaa tataatgaag
     2821 aaagtaagtg tcataatttt attatcttca gttcatttac tcgcaaatat ctaatattaa
     2881 ggttcttcag ttttttcttt atatctttaa tcatttttat tggttattta attaagaatt
     2941 tccttttcta tctttccatc tcttataaaa atggtcttat cagtttctag tgctagctct
     3001 tgattatgag ttaccagaat aattgtcttt ttatattttc tggtaagcat tttaagtaaa
     3061 tcaaaaacct cttctccagt tttggtatcc aagtttcccg ttggttcatc cgctaaaatt
     3121 attgttggat cattaattaa tgcgcgcgca atagcaactc tttgttgctg cccaccgctt
     3181 aggttcatcg gtttatattc tgccttgtct tttattccta atagttccaa taacttttga
     3241 gaacgataat ggcgcacgct tttatccatt cccatgatta tagcaggaat ttcaatattc
     3301 ttttgaacac tcgttctatt aattaggtta aatgattgaa aaataaatcc tattaagtta
     3361 tttcgcattt gtgcgatttc agattcatta agagataata tattaaatcc atttatgata
     3421 acttctcctg aagttggttt atccagtacg cctataatat taagtaatgt agatttgcca
     3481 cttccacttg gtccaacaat tgatattaat tcccctcttt ttattttgaa attaatatct
     3541 tcaagaacaa cagattctcc tgctggagat tcaaatattt tggttatatt ttttaattcc
     3601 attacaacca tatggtcatt ctttacatct ttcgttttat tttcaccctt tatttgattt
     3661 tcaataggac aagaatcatt ggtttcttaa aaagacgtgg ttttatcaat ttcggttggg
     3721 taccattata atgataatta taatttttta ttgtgtccat ttagttttaa gatgctctct
     3781 agaactaata ttagtatttt tgttttacct aaaatataca aagtaaaaag tcctcccatt
     3841 tccatagttt tctcaaagaa tgttgcacta ttttaaacat atattatgaa atttgttctt
     3901 gacacatatc tagcttaatt attctgaatc tcttcgcagt ttcctgcagt atcatcactt
     3961 tcatttaaag taaaatcaat tataacatct actccatctc cacaatcaaa ataatctgca
     4021 ccttttagcc cagaaagaat atcatcacca acatccccgt acaatttatc atttccgtta
     4081 cctcctatta aataatcatc atcaactcct ccagctatga cgtcatcatc ttcttctcca
     4141 tgcaaaaggt cactaccact tcctccttga actatatcgt ctccttcatc accgtataac
     4201 tgatctgttt cttcatttcc ctgtatttta tcattgcctg ttaatccatg tacagtatct
     4261 gaaccagata atgcaactat tatatcgtca attgcagtac ctgttaatcg atcattacca
     4321 aaggaaccaa ttatcaaatt gttatctaat ggttgatttt gttgttgttg ctgtaatggt
     4381 tgattttgtt gttgctgctg taatggttga ttttgttgta tattcgatga tgcattttgg
     4441 tctaatactg atgcttttat ttcgtgaaaa actagaattt gaaatgtgtt attaggagtc
     4501 aatatactaa tagcaaataa agcacaaata ccaattatca tcagatagga ttttataaaa
     4561 aaacgcattt ataaataata tattagtttt ccttattaat attgtactgt tatatataga
     4621 attatacttt aaaatcatat attttatttt taatattgtt attataaagg tagtagaaaa
     4681 caggaaatat tacttacata gtcactgtta gttaacttat ctatcattat ttattttacc
     4741 ttttctagtt atcgataaaa tataggatat agttataaaa aataataagt tacttagaaa
     4801 acttaagata ttatgaaatc tcattaaatg tattgatttg acaatagaaa aacattttat
     4861 tttgaaaaat ctatctaata gagattatag agctaaagca tttacaaaat taaattcaga
     4921 tactttatta tataaaataa ataagtataa ataaattttc tccattaaag gttttaacat
     4981 attaatccaa aataaaactt aattatactt ttttatatga tataatgatt ttatgtaata
     5041 aatattatct caaaagttag aatctaaaat tgtttaagta aataacaaat taaatccata
     5101 aaaattaata taatatatta tcaaaaaact cccaaggaat attatacata aaaactaatt
     5161 gattacttga tttttttctt tttaatctta gaaaatataa agatataaag aattatgaca
     5221 tcttctgtac catccatttt ccactgatat ctgtaaagaa aagtcgagat aattaaccat
     5281 accatattat taaaaagaat agaaatgaga aatccaaaaa aataaattaa aattataccc
     5341 gtaatagtcc caccaacaaa taacgggatt attcgtttta atatagattt taataagaca
     5401 tccacgaatc ttttacattg ttattatcac aacatatttt attagaatca atatactggt
     5461 agttcgtata tatgactcta ttaatatgaa tcataatgaa acacgtatca aatgaagata
     5521 attacctcaa ataggatgtt ttattattat aatattatta ctattacaaa attataaatt
     5581 acataccatg attttatttt gtatgagcac tgacaatgat aatttagatt ctgatggaaa
     5641 tgaaataata agtcctgatg gaagtgtatc tccagaacta gaagatgaac cagagaatat
     5701 taaatctaaa gaatccaaag attattcaaa ttctaaagat agtattccct cttttaaaaa
     5761 aatattagtt gctgatgacg gtaaggatga atccgatgca gcacttttgt atgcagtctc
     5821 tttatccaat tctacagggg cagaattgct cattttacgt atattagaag atgttgaaaa
     5881 atttgaacat gtcacagtgg aaggttctca tgaatcatca aaaatggata attatgactt
     5941 tcatagacat ataaaaggag atgtaataga tgctatggaa gaaaaaatta agaaatgtga
     6001 agcagctgga tgtaaaaata aaatttcata taaatttaga gcagggaaag ctgacgatga
     6061 aatagttaaa gaaattaaag agaataactt tgatctattg attttaacaa cctctcatat
     6121 cgactcatgg ataaggtcat tattcagtga tgctcgaaaa ataataagta acataagcat
     6181 acctgtatta ataatacagt aaacttataa aataaaattt tctggatgca tactttgtta
     6241 attctttcta ttaaaaatgt ctctaaacaa taacttacgc ggcttattag aaaatatcat
     6301 agatgaatct ataataatta tgcctgcttt gatgactctt tttagaaata ccattccgcc
     6361 agagtttggt atacaacggt tagaagatta tgcttttgga tatgttcaag gtttgatatt
     6421 gggacaattc ataagttcat atcgagctat ttttgcccaa aatctacaac cagagatatc
     6481 cgcggaagca ggaagaatcc ttctaagaag atcgagagat atacgtgatg caatttttaa
     6541 ggctggctaa aaatccattt tgtcatatct caaaatcttg tataatataa atctaaatat
     6601 ttgtatacaa attcaccatt gttctgacta ggtagcaaaa attgtactca attgtcatag
     6661 cctgactccc attcattggt aatgaagaaa gctatgaaca taaatcattt cacatgatat
     6721 ttataaaatt agctatgcca ttttccttca tataggctat taatttaata ttaataaaat
     6781 aatattataa ttgagaattt ttgaaattat ctgaacatta gagaaattac atataaggat
     6841 aggataaaat ttaatacaac taaaccaatt aatattaata aaaataaatt ctttttcata
     6901 attctttctt ggtacgtctt gttgtaagat ataaagttac ttatatttat tttcaaaatg
     6961 taattgtaaa tttatagtct gaatcctcct ataacttgaa gtaataattt aatttcttat
     7021 tatagaataa tttttaatat taaccataag tgatattgat tatatttcat cacaatgatg
     7081 tctttttctt ttttcatcat tgttttacaa ttttaattat ttcttctttt ttaccaatcc
     7141 agaataccat ttatgtaatc aatatgctac atagtatggc tcataacctt agtggagtca
     7201 attcgtcctg tttcagtaat aagatgaagc aagagtacat tatattttgt gacaggatta
     7261 ccgagtttgg atctgaatat tacagttaat atttatttaa ttaataaatc ccgtatttca
     7321 atggttatgg ttattgttag aatatataat cataaatctc gcgctcatta tggatttgct
     7381 tcctagtact atggtataaa catctttaaa ctataataac atgtttttta tttgattatt
     7441 tcaaatgaat gacgaaatag ccataataaa ttttatcaca acaatagaaa atattaattc
     7501 tataccagtc ccagaaagta atcaagagta catgtgtgaa ttcataggta aaagagtatt
     7561 gattactgtt cgtaaaagaa aggtaaaagg accaacgatc gaaggtgaaa aacctgagga
     7621 taacgaataa attaaaaatt aatcatatac gtactagttt attttttaaa aaataatgat
     7681 actctctccc tgacctaata gtactatata gttaattaga cataaaaaat taaatttaga
     7741 atatgaattt atcatcatgg tataaaaaat ccgcgcctct atttacggct aaagaaagta
     7801 aatcaattct tcatgatttt tttgtatatg gaaaagaagg tgtaactata aatccatata
     7861 atggttgtca acatcggtgc ggttattgtt atgctaccta tgagtggtca ccagaatttt
     7921 atgaccatat atatgcaaaa atcaatgcta cagatttact atataaacag ataatatctt
     7981 ggaaagggaa aataattact cctgtaatga tagcctcagc aactgatgcc tatcagccag
     8041 cagagctaaa atattctctt actagaaaat gcgttgaggt cctccaagag ttcgaaattc
     8101 catattatgt ttttacaaaa tctagtctaa tagagagaga tttagaatta cacaaacgtt
     8161 attcaaataa ttgttttgtt gtatggtcaa ttactacaaa caatgagaat ataaaaaggg
     8221 ttattgaacc aggtacacca acgtctaaat cagttttcag agtgatagaa aatttctaca
     8281 aatcgggtat aaaatgtgtt attaatattg atccaattct tcctcttatc acagactcta
     8341 ctgaaaatat agcaagtata ataaattctg caaatgatat tggagtagaa tatgttagtg
     8401 gagcgatttt aagattaaga tatgatatat gggaaaggat gaaatatatt ttacagactt
     8461 tgcaaattga taactcgata aaactttatg aaaaaatctt taaattcaaa gatcctatca
     8521 actttaaaaa aaatctaaac actgaaagag aatactcagt ctcaataatg aattatttgg
     8581 aaaatgaact ccagaaaaga ggtatgaaat tcggatatcc taaattaaac ataaagaaaa
     8641 tatcttcttc ttcttctttt ttaaaaaata agaaaaatat agtatcgtta gaagatttta
     8701 tgaaataaaa cttgaatttt tattagcttc aggaataaaa aattatattg aagattatat
     8761 ttataaatat tagaaaaaat aaaattatgc attcgaactt catacaaaca tatgagattc
     8821 ataatggctg cttctaatta tattattttt ctctctttgt tagttctatc tctaaataat
     8881 tctgaaaata tttttaatga tgcatttgga ttttttcatt tagggaactc ccaagatgat
     8941 tttaaacctt atgaaaatac aaaactaaag ttaagtatcc aatatccttc aagttggcaa
     9001 aaagaagaac gtctgaatga tttcgttacc tttattgcac caatcgtaga ttccagccaa
     9061 tataaatctc cagcaggatt aggagtatcc tcattaagtg tgagtaacgt atcactagct
     9121 acaatcacaa atgtccacct aaaaaatatg acaaataatc ttaaagattt tcagttggta
     9181 gaatcttctg atacagttct tgcagataat agaccagcaa aaaaaataat ctttaccgca
     9241 actgatgatg gagaaaaaaa acaagctctt caacttatta ccaagaataa tgacaaagta
     9301 tacctaatta cttacaaagc aaatgtcgat aaatacgaac agtattttcc aataatccaa
     9361 aaaatgctta attcattggt ctttctcaaa taattatgat ttacttttac ctattagtta
     9421 tgataggtgg ttacaactgt agtatgtagt aattttataa atccactgtt atatagaaat
     9481 atatcattat cattatcatt tattcaattt agttgattga tgtgattgtg tggactgtat
     9541 tcaagaaaaa ttgaatttgc ccctgttcat aactagtaca aatttaaact ctgtgagaat
     9601 taattgccac tttaattaat attaattctt ttattaatta aaaggccttt tatgcaataa
     9661 cagtaggaat aatattaaca tatcttgact aatgggaaca tagcttttta gaaaataaaa
     9721 aataattgaa tacaaacaag ctaattaatt tttttagggt cttattgtgt atgtgtagat
     9781 tcagtggcgt tttctgtatt ttctctatgg gtattctccc cttccataac ttcatttatg
     9841 ccaattacca ctgatgaagt tggtggaaat tctatctcat cttctgtagg attagacagt
     9901 gcgatatctg ttattgttgt tacatttccc gtaggtatca aatcaacgtg atagagacat
     9961 tgctcccctg gagttgataa agcagttata ttttcaagag ttgctgctgt catattaggt
    10021 gcagaaccga ttaaaacctg gattggagaa gtactatcag ctccacatgg ggcttttaaa
    10081 gcaatatgtc cattcacgat atgagatgga gtagaatcat aaagatgtaa aaaatctgtt
    10141 cctggaataa ttgtatcttg taatagtatt gtaactgaat ctcttacatt attagtttta
    10201 ttgtatttgt tcatttctaa ggaatgtctg gcttcagttg catattcctc cacttcctct
    10261 tcaacccgag tttgattgct ttcttgtgca taagatagag cattattata gaggccggct
    10321 acaatagata gagttaaaat tgcaataatc ctgtattttt ttataatttg catcaatttt
    10381 tctagagatt ttttgttatt atactttata gtacatcctt cgaatagtct taataatcac
    10441 ttattgttgt gcgtattttt tatgtacgtc tagaaccaag acatatttat taagattaat
    10501 ttttttatat gttataacta taatcattca ttgcaagttt accataatat atttatgtta
    10561 attattagta ctgtttttat tgttaaggaa ttacaataat cctgatgcat tttccaataa
    10621 agctgggtaa ttggtattaa ttgcgagtat cgttacttca ttatctaatc ccatttattt
    10681 tacctaggct tttgtgactc tagtggttgc tgaatatgtc tatttattga attctactga
    10741 agattttcat acgatattta ttcctcacac taatattgat gtttctcatc taatcttgta
    10801 gattatgata tattttttgg agtttctttc ttaaaaagtc cgtggtgaat tctatatggc
    10861 tagtaatgaa taggaaaaag aaataaattc aagaaataat aatttgatga tttgttattg
    10921 attcatacta aataatatat atagtgcttt taagaagcta caggactctc taacggtgtt
    10981 taacgtatca tttgaccgat ggataattct tcaaaggttt tggtaactag agataataac
    11041 cgattgacca gatattttct catgatagtt tcattaataa atgtaattta ttagactttt
    11101 acgtccgatg actattagaa aagttattat ctatataata gaataggggt ccttgctgac
    11161 ttaatgacca tattagggat acaaagattg acttcaacta tttcatggga tttgtcaaga
    11221 ttaaaagaag caatataata aagatacata cacaaaatga attcaaaaat tatgatattg
    11281 gaataagcaa actaatactt caaacagaac ttttatctaa atatacgagt agtattacaa
    11341 aaaaatatta ttgatgattt acttatatga ttaatgaatt cttaatattt ttaaaattat
    11401 tgtttctttg tttttgtgta tatagatctc aattaaacag aacaaaagta acctagagga
    11461 ggccaccaac aatgaacggc tagatgtact agaatttatg tgcaggtcta aaccaatcaa
    11521 tttattcatt taacgttaaa aaagtttgta ctacattcta taaggatatt tattttttca
    11581 ttagatttga atattgttta aaaatagttt gaaatatgtc aggatggatc ctttaaaggt
    11641 atagtccata tttccatcta gtcttttcca tatccattaa aaaagatgtt aacatatctt
    11701 ccaaaatcct acaaataacg tccccttttt tttctgaagc attttttgga gaccccgttg
    11761 cacctgtttt agtaagttca tccattgtcc aaaagattct taggtgtttt ggtaaattag
    11821 gcatagtatc ctcaggtata ttatttttat ttactaaatc tggcctaact ttcattatag
    11881 ctgaagtttc agcttctccc ccatgaccca atccatccca tacattaaaa gtatcagggg
    11941 aaatttttcc aattaattcc caccaagatt ccaaacatgc tattgtaata tttggatatt
    12001 tatcttttgt atttctagag gcaatctcta taggagcgat atttccatcg tggccattaa
    12061 tgattaaaat tttatatacc ccattgttat atacactttc tagtaaatcc tcaattatac
    12121 taacaagagt atttgtacct atactcatag tcatataaaa ttttttatga tgagtactta
    12181 caccatatgg aataggtgga aagattatta aatttttgat ttttatagct actctttttg
    12241 ccaattctaa agggaaaatg aaatcggatc caaatggtag atgatttcca tgattttcac
    12301 atgacccaaa gattaataat gctactgtat cgtcatttac tttttttttg atttgatgag
    12361 cattcatgtc cccaattaaa aagaactcat tatctttcat attcatttta cgttattcaa
    12421 atattttttc ttattgatta gtattataaa acaacaagtc tagatcttat ttttaacaat
    12481 gagaagataa aattgattta aaaaataaag ttaataattt cattagatac caataactga
    12541 aatgggtata aaatattaag tatctattat aatacttctc ttggctttat tgacagtctc
    12601 ttgtatttta taccggcata tgcaggatga gaaaatgcga ccaacacgtc cctatctaat
    12661 tttactgtca ctaaagtaat taatcaaaaa actgtttcat ctattcctgc ttcagttagt
    12721 gctccaggat tgaaacgaca ttaagagcta tttgctttac ccttacgaga tgatggcaaa
    12781 ctttggtgag gaaccgtaaa attaacagct acccaccaat acaaatgaaa tacttcataa
    12841 ctataaccaa aacatttcaa ttcaactgat aaaccttaca atactgtcat aggagacaac
    12901 agaattgcaa tttctcggtt aagaaatgtt gtataactgc cactttaaat aattcgcaaa
    12961 ttagttcagg cacatgtgat tgtgttagaa gtgcactggc ttttcagaaa gctgatttta
    13021 cacccccttt ttctttaact tattttctat atgcaattgc taaaggtgtc cataatttat
    13081 tttatgtaaa tttattatat tgaatatggt agtaataatg atttttctat atatagatga
    13141 ttcaagaatt aatataaaat ggttaaaata tatagatgag gttaaattag tacaaaaata
    13201 gtaaatctat atagatttat ttgaaagtga tcatcaagaa atatacataa ctaaaattta
    13261 taaaatgata ttgaatcata ataggttaat tttcaaaaag actgtcgaga agttatacaa
    13321 tagaataaca ttcttaatta tagcagcttc cttggtaact ttaattacaa ttaattatag
    13381 catggaagat tctcttgcaa ccgaagattt taccaataaa actcatactc tttcagagtt
    13441 attcaaaaat gtggaaaagt cagtagtgca gatttctagt gaggatgaaa ccaccgagtt
    13501 gttaggtagc aggttaggtt caggatttgt gtatgacaca aatggacatg ttgttaccaa
    13561 caatcatgta acttcaggag gtaaagatct tcacgttaca ttttctgacg gaactattta
    13621 tacaggtaaa gtaataggtt cagatccgca tagtgatctt gccattcttt taatagatga
    13681 tgttccaaag gaaaaactat ttccattaac actgggtaat tcatctaatc ttattgttgg
    13741 agaaaccgtt gctgcggttg gtaatccttt tggcttatct ggttcactta cagagggtat
    13801 tattagtggg ctaggaagat tgcttccatc aacaccagaa aatattttcg gaaatgatcc
    13861 tagagtagca acattttcaa tacctgatat cattcaaacc gatgcagcta taaatcccgg
    13921 taattcagga ggtccattat taaatcttaa tggtgaggta atcggaatta attctgctat
    13981 attttccaat acaggggtat acgctggagt gggatttgca attccatcca acacattaaa
    14041 aaaggtaata cctgaattat tgaaaaatgg ttcttatccg catccatggc ttgggattac
    14101 aggagtagat gtcacgcctg atattgcaaa gaaaatgaat ctaaccgaag cacgtggttt
    14161 tctcgtaatt gacgtaaatt ccaatggtcc agcagataaa gcaggaatac gaggtggaga
    14221 taaaattgat actatagatg gaagagaagt tgaactcggt ggcgacatta ttctagcaat
    14281 tgatggaaat cctgttagaa aaatagatga catactttct tatctagaaa gggaaaaatc
    14341 agttggagat aaaatttcat taaccattat tagagatgga attgttcaag aaaagactac
    14401 tgtattacaa gcacgacccg attctcaaac tcttattagt tcatcacaac atccttcgct
    14461 tggtattaca ggaatcgacg taatgcctga tattgcaaag aaaatgaatc taaccgaagc
    14521 acgtggtttt ctcgtaattg acataaattc caatggtcca gcagataaag caggaatacg
    14581 aggtggatat atagttgaaa taataaataa tactcaatac agattaggcg gcgatgtaat
    14641 aattggaatt aataatcaga ctataaattc agtttcagat ataactgatt atttgaagaa
    14701 tacgaaagaa ggagatattg taaaactaaa aatattaaga gataacaaaa tgataaatgt
    14761 tcctgttaag ttggaaatta ttacaatgcc tttagatcaa ccggatgaaa ttcctccatt
    14821 taatgacaat cctaaagatt ttggagacga tcaagggaaa tttactgaac agttaaagaa
    14881 agaatgtctg ctgaatttta acgaaagtat ctgtaatttt ctgtttagat agacagaaaa
    14941 aaatctcttc tttttttata aattgatgta aacttttctt attatttttt agattgagaa
    15001 actatgaagc aatttttagt aatagtgata tcattaataa tattactatc attttattct
    15061 ttgatattac caaatgtgta tggtaatggt ataagaaata attttggtat cgattatcta
    15121 cccccactca atttagaaga tactcctcta agtttacatg ttgaattcaa accatctatg
    15181 actaaatcaa atattaatga aaaaagatct ttgatattcc aattaataga ttttgataaa
    15241 aatataacac atgtgcctga tttttacaac attaccatat taaaaagtga attatatgat
    15301 gggaaagtta tcgaaaatat atttctaaac ggatctttca tttcgaaaaa tggaacttta
    15361 atcctagaat ttgatcctaa tgaaagcagg aatgaaaata ggaataataa tcattatata
    15421 attgatagag gctgggatga gcagaggcag cagcatcaga tgaacgctga taaaaatgga
    15481 ataatactta ttgatagacc gaaactggat agtggaatgt acagattaaa agtaaatgtt
    15541 gctttatctg actattctga tttaaataag aatataaatc taaaatttga ttcctatctg
    15601 agtttaggaa atatactgga ctttgtaata gataatccag ataaaaaaat aaccatcttg
    15661 tcgttcaatg ataaaatagt aaattatggt tatgatcaac aatctagaaa aatttcattt
    15721 gaaattccat ttaaatataa catgacaaga attgaagagg gtttcattta tattcatatg
    15781 gaaataaaaa ttccagatac atttaatgaa ttatttaatg taaaagagta ctggtctaca
    15841 attaataata ttgaccatga gaaaataagc tcagcatctg tttctgtaga tagatattct
    15901 aactcttcta atgtaatggt tcattttgtt ttagatagca atagtctttt taaattaaca
    15961 aaagaacgag aggagtatcc tacagaggag aggtcagaaa tgacgcttcg attttcccta
    16021 caacctaaaa gtaccatctt aaatataaat ggatagatat tgtaatggat acaaattgca
    16081 tttctaatat actgtatgaa aagattattg tttaatcata attacaatta attattataa
    16141 gaattattag aggttgtatg tatcggtcga tcaaagattt agtcacaaat tttataacac
    16201 acctaaattg tcataaaaaa ggaagatatt actttatttt atgtggtttc ccatagtgat
    16261 cctcgtaata tccactttca tattggtctg aatctcctcc atatctatct tgattgtatt
    16321 gcatatctga agtgtatcca ttggctggtg gataatcttg tgttggtgga taatcttgtg
    16381 ttggtggata atagtgttct gatctgtctc cagatgctga cacatcgatc ataggtgcgt
    16441 tgttaaatac caaaaatgct cccaaagtta acgatgctaa aataattagt gaatttgttc
    16501 tattcatttc tttgtataca gtcagatgaa aacattgtat attaagtgaa ttcaaataca
    16561 ttatataata agtttagata accaaggtac atattgttct taagttactt taattccttg
    16621 caagtagtat ttaatttata tagattatta ttcagactaa tatacaagaa attttttact
    16681 ctatattatc ttacaaatat tcttatgaaa gtgatgaata aatcattgga aatgaagtat
    16741 catatcggaa acttgtatta gagtgacccc catcaaatgc ttcataataa tgtttaatat
    16801 ccatattttc tagtttttgg tgaagtattc ttgctccaat gtggaggttg aattcatcct
    16861 tggttccgca atctaggtac aagaaactca atttcttgag gttattttga tactggctta
    16921 tcattttaat gggatccaat ctcttccaca ttttccatgt gtcgtcattt aattcaccag
    16981 tttcgagatc aaaaggaatt ctaacaccta atggtgatgc tgggtcggga gaataatgtg
    17041 ctgccataca taaaatgttt aaggctagaa aatcactttt ttctttttta ttcggtttat
    17101 tccagaattg attgaaccag tttctgattc ctccggcttg cttgaaactt gcataagcgc
    17161 ttggaaaatc tggtaaataa caatattcaa atgcggcatc tccagaatgg caagcaatcg
    17221 ccttgaatat ctcgggataa cgcattccta aaacaagtga accataccca cctgacgatt
    17281 taccccatac gccaaatgac gaaatattgt aattattttt tatgaatggg attatttcct
    17341 taacgatata gtcttcatat cttcctgtag ctgtagaatt tatatattga tttcctccaa
    17401 attttgtaaa acaatctgga ataactaaaa tcatagaacc acattttttt tcgcaaatta
    17461 atctattcat tctttgttca acgttttcac taaaaggatc tatattgaat aaagttctac
    17521 cattaccacc aaaacctgaa agaccaatag ctgcagggaa ccctttggat cgactttgtg
    17581 aataatcatg tgggacatat actataattt ctctattgtg tggatctttc aaaggattag
    17641 attttaatac tgaactttca aaattatgaa taattatttt tccttcggta acagtcattg
    17701 ttgttgatat tacaatttgt ctatatatat aatgtacaaa ttaaattaaa acctgctttg
    17761 gaaataaagt aaatctatat taaaattaat agtaggtgga atctcttata cttccatata
    17821 atctatatat tccgcctgta gcactagata tataattcca tacttctgta tttataggca
    17881 aatctttgaa tctttctttt agattattgt caacagaatc atatactaat tgtcctatta
    17941 caatctgatc agattccgca agtgatgtca ttttaactgc tatacttatt gtgtatccta
    18001 aaatatcgat aataggtttt tttgttatga tattttccgt gccatgtttt aatacacttg
    18061 tttcccatcc atattgaacg acgacatttt ctcctacatc aactcctatt ctagcatgga
    18121 tctcaggata atcatattga cttaagatag gattaattcc atgtctaatt acagtggtca
    18181 ttgagagggc acaattaact gcattaatgc aagggatttt atcaaaattt ttatcagatg
    18241 atctcactaa aaaaaaaccc aaaactgcat ctccaacaaa ttttattatg tgacctccat
    18301 aggatgctat taaaatggac atttcttgag tgaaagctcg tataatgtct gccaatctgt
    18361 tagccgaaag attcatacaa agctttgtaa atcctactaa atccacatgg ataataacta
    18421 gtcgtgtctt agattcaagc aaattttcca aaatttgtct agaatcctcc gttgaaatat
    18481 taaactcctt tgcttgcaag gcagaccatg ttcttgcttg agcattctta attaattcat
    18541 taatatctac tatagcatca agataaaatt ctgctaaaga tgaaggcgaa ttggaaatat
    18601 tttgatctga gtgtctttgg tcttcttggt ttactatatt ttggacctct tggacgtcta
    18661 aatctgtttg aaatgcaatt atttcaaggg gaatccctga cttgaaaagg ttcagaactg
    18721 tttgtttttg ttcaattgat ataacttttc accttttata tattataata aaatgatcta
    18781 aataatgtat tatggaatgg taatgttagt taacatgttt gatatatggg caatcttcta
    18841 tcatcataaa ggaaatctaa atagttcttt ctgttcaatt gacccccaag ctcccattcc
    18901 ttcgccttga gcatttgtcc ccacaatctc acttactatt acttctctag ctgccattgg
    18961 aggccatgtc atagctacat attctccaat ctctcctgct ttttctaaag atgccatggc
    19021 tattttctct tcatttaatt ttttttctac caacatattt ttgactttgt cataattttg
    19081 tctatttcca tgaacaatta catgaatttt tttatcatta tttagtgatg tcatgatttt
    19141 cttgcataaa ttatgtataa aagcttacct ttctattaac cttatttcgt attttaaaat
    19201 aaaaaagatt tatctatagg acctattgtt tttcttaaaa ctttgcacaa aatatttaaa
    19261 aacaagtaaa gaatttctaa tttttgtata gtattgagca aaaaacccga gacctattgt
    19321 agcaatacct gcaactatga atctcattcc tactatttta aatccttctc catgttcaat
    19381 actttgacct aaataaatta aaaaaaatcc aaaagttgat ataattatac ctgagactac
    19441 taatggtaaa aacaatgaat gaaatctatt tttcttcact tccactaatc attgataata
    19501 actcaacaaa tttataaaca attttattga ttatttatta tcttttgtta ttggaaagtt
    19561 tgaatatagc tgttcttgga aacaatgaat tccttaacga gtttggtaaa agaggtactc
    19621 tttctgatat agtgctttat gacagaaaat taaatgatcg aatatttact ttgattgctc
    19681 ctatttcttt tccagacaaa attcagactt tacctcaagt cttgaatatg acagatttcg
    19741 tagtgttaaa tataacacat ttggacaaat atcttgcgga acaaataatt gctataagta
    19801 gcctagatat tagaaatggc tttttgcttc attcttataa tattgacgaa caaagactta
    19861 atcagatgat taaaggtact tcaattgaaa attttaaaaa aatagaaaat ctagaaattt
    19921 taaaagagga gataaataat ctccactcta agtcgaaaga gggagatctt ttaatgccaa
    19981 ttgatcatag ctttgacgtg aaaggtgttg ggacagtctt acttggggta ataaaacaag
    20041 gaagaattag agtatatgat aagttaagaa ttctgccttc aaataaagaa gtcttaatta
    20101 aatctattca aatgcatgat gaccccgtag atgagtcttc aagtccgtca cgggttggtt
    20161 tagcggttaa aggaatttca ccgagcgaaa tagaaagagg ggatattatc tgctctggtg
    20221 ataccataaa gaccgcagat accgaagtaa tagtcacaga tttttcaaaa aataaatatt
    20281 acgacggtga gattacagac acacaaacat atttactttc agtaggtcta caagtaaagc
    20341 cactaaaaat tttaattaat aattctaaaa tattgttatc tttagaaaaa cctattgtct
    20401 attatgaaaa acaaaaattt ttaatattga aaccagatag taaaactaca agaataatag
    20461 ctacaggatc attcccttca gtaattcgat gagttaaaat gtgattaaaa cttaaggttt
    20521 gcattagtta ataattgtta gtactttata atgtgacttg aataattatt gactaaaaac
    20581 ttaatatatt atcattattt ttgcggctgg tgaaagagtt attgtattga aaaatgtatt
    20641 tttcattttt tccggataaa agtacttttt ctaaacattt ttttgcattt ttattcattt
    20701 taaaatgatt tttaaggtta ttattttaaa tggatctata tcaatagtaa ttgtatcatt
    20761 ttctatcttt ttgatttctt gaatctcatt ttccataaag tctattaatt tggtctttct
    20821 tatttttgct gcaagtttta tttttgtaat tgtttttttt ccttctgttt cataaactct
    20881 taaaataatt ccctttttat catcacttaa cttcatcgta ctcaatatca cattactttc
    20941 tgagatttca agaaatgatt tagaatcatc taataccttt acgttattcc tcccattatt
    21001 ctcagcaata ttattttttc taccattgtt tttctttatc tgtattggta ttaacggcat
    21061 atttatttcc gatccatgtt ggtatattaa atcctcactc caatcgtttt catgaggtaa
    21121 gattgaatat ttaaaagtgt atggtttcac ttctgctgca tcgggtgtag gtatacatgg
    21181 tcccattata ccatcagaag ataataatac tatactacgt agtaacgtaa tatatattga
    21241 tccgtttctg atttcgtgag atggcaaacc ttgatgaagc agactaattg ccacggtttt
    21301 attagagtca ttttttatag aatagtcaat ccattcaagt gagggaaata ctcctgaagg
    21361 tttctcaaac cacttgctgc tatctttttg atcgtaaaga tcgggttttc tttcaacagc
    21421 accaaattgt gttccacacc aataactttt agctgtgcct ttagtatcaa actttactct
    21481 gagtcgtgag tgaggatgtt tgttaaatat atgagttaca aaatctattc tgtttaaatt
    21541 cttgtaaatt attatttctt tttctatatc tatgaaatcg tgtctataca tcattggttt
    21601 cagcttttca gtaaatctat agggccatcg tagtgcaaaa tatttgctct taaacttgat
    21661 atgggatttc agttttctat gtattacttc atatttttct ggtttaaata ttccatattt
    21721 aatcccatcc ccagtttctg atttaaataa ttttagattt tctctatgat aataaagatc
    21781 tcctatctct tcatctaata ttatttcatt tccagtacaa tagtataatt ttttgttatt
    21841 ctttatggat ctaaaaactg taataattgc attttctcta tttacttcga tttcgtattc
    21901 tttattttta aaacttgtat cgtgagcaat tagagtttcg ccgtctattg tatctgattt
    21961 tactatctct aaagttttgt atcccaatgg aggtacagaa ataatacaac ctatacttat
    22021 ttttttaata gagttatctt gatataaaga ataatccaga atttctatct ctataacctg
    22081 atctccattt tttaagcaac ttataccttt gatttcacct tcattaaatt ctaaaagtat
    22141 ttctgtccag tttgtaacta accatgatag aggattaaaa ataaaaatgt cgttatcata
    22201 attagtcatt aatgacaatt ttctcaaata ttcaaaaatt gatctgtcta tactttttct
    22261 ctcattttca aatatttccc taacttcatt gtatatttca tcaacgcctg tacctggtaa
    22321 aacatcatgc attgcaataa atagtatttt cttccaatac ttatgaagct gatctgaaat
    22381 attttcgtca tgtatattaa ttaaccaagc tagagcatct aatctttcga gaagtaaaat
    22441 actgttttca aattctttca ttccttgttt tagccacata cgtgtggatg tacaatcagg
    22501 atacacagca gaaaattttc ccgaatacat ttctcctttt cttactttga actttatgtt
    22561 ttgtttttct aagtaattaa aatattgaag aggagaggac atgatcatat tatatccact
    22621 tttaggtttc gttacttttt ttccatttac ttttttattc cattctttta taacctcaac
    22681 taattcaggt tgtggaggag tagagccgct tccagacaac attaaaattt gattagttga
    22741 agcatatttt tttaattgtt tcaacgaaag ggatagctga tctaaaaaaa gtccagcacg
    22801 atagcctagt ggcatccaat gagtaagaat ttgagtccca tcaagtcctt cccaaaaa
//
LOCUS       DASVEV010000060         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1181374, whole genome shotgun sequence.
ACCESSION   DASVEV010000060 DASVEV010000000
VERSION     DASVEV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181374"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF222_03190"
     CDS             complement(<1..373)
                     /locus_tag="VF222_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HEX7141465.1"
                     /translation="MVDFGYLLVLGISAFVSTNIDEIFLLIVFFSNSLKFSKYQVVIG
                     QYIGIGLLIAISIIASFISLVIPSFLIGFMGLIPITIGIKKLTMYYKKKKETDPNSTK
                     YKFTKNGSALSFINVAAVTFSN"
     gene            521..1351
                     /locus_tag="VF222_03195"
     CDS             521..1351
                     /locus_tag="VF222_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7141466.1"
                     /translation="MNRTFMIYILAAIISFSAITGITMQPISVSAQTQPAQQQPQTPA
                     QVQPLAISPSCGQVITQNVVLTSNLNCAETDGLIVNAGDIVIDLNGHTISGPDIDSDT
                     QTSSKVGIMIPNVNNVVVQDGTIEGFQAGVLMTGSQNVEVKGMVIKNNQIGLFSTGAS
                     IINAHLSIIMNNQIGIAGHSTQQSTFEDNIIFQNNLAGITLVNSDNSVITLNSITESG
                     NGLYIDNQSNQNNINFNNVLLNTIDVNNANGLPVNTNGNTYDQNNCMTANPSGICIGK
                     "
     gene            complement(1588..1758)
                     /locus_tag="VF222_03200"
     CDS             complement(1588..1758)
                     /locus_tag="VF222_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030515 - snoRNA binding [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleolar RNA-binding Nop10p family protein"
                     /protein_id="HEX7141467.1"
                     /translation="MKHILRKCLMCRIYTLKSRCSHCSSLTVDPTPAKFSPEDKYILY
                     RLPESYNKKESN"
     gene            complement(1760..>2204)
                     /locus_tag="VF222_03205"
     CDS             complement(1760..>2204)
                     /locus_tag="VF222_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7141468.1"
                     /translation="IEEKILTKYDYVYDIFEATTKKGKDVLLQLELTENVIKIIEDES
                     KKIPIPHVEIRGILEISSQKSNGVEIIKSILSTAETTKGNSKIMISYIAAPKYRIVVA
                     ADNFKIAEKSINNSIEKIKSSIEKEDGHFNFIRQESKKTQQQLVH"
BASE COUNT          838 a          321 c          290 g          755 t
ORIGIN      
        1 catttgaaaa agttacagca gcaacattga tgaaagataa tgctgatcca ttttttgtaa
       61 atttatattt tgtagaattt ggatcagttt cttttttctt tttataatac attgttaatt
      121 tttttatacc aatggtaatt ggtataagac ccataaaacc tataagaaat gatggaataa
      181 ctagggagat aaaagatgca attatactga ttgctattag taatccaatt ccaatatatt
      241 gaccaattac aacttggtat tttgaaaatt ttaaagaatt agaaaagaat acaataagta
      301 aaaatatctc gtcaatattt gtggagacaa atgctgatat acctaatact aacaaatatc
      361 caaagtctac cataaaataa gcagctactc aattttctat tgatgatagt gatataaatt
      421 gaattttaaa tcaaataaca gaaataatac aataatttaa attttaataa tggttcttta
      481 cggattaaca taaatatgtc agtatcaaac catctactaa atgaatagaa catttatgat
      541 ctatatcttg gcagcgataa tctcattctc tgccatcacg ggaataacaa tgcaacccat
      601 atcagtaagc gctcaaaccc aaccagcaca acaacaacca caaaccccag cacaagtaca
      661 acctttagca ataagtccat catgtggtca agttataaca caaaatgttg ttttaacttc
      721 taacttgaat tgtgcagaaa ctgatggtct tatagtaaat gcaggagata ttgttataga
      781 ccttaacgga catacaatat ctggtccaga tatcgatagc gatacacaga cttccagtaa
      841 agttggaata atgataccaa atgtgaacaa tgttgtagtt caggatggta ctattgaagg
      901 atttcaagct ggtgtattaa tgacaggatc tcaaaatgtt gaggtaaaag gaatggttat
      961 taaaaataac caaataggat tattcagtac tggtgccagt ataattaatg cacatttatc
     1021 tataattatg aataaccaaa ttggtattgc aggtcattct acacaacaaa gcacttttga
     1081 agataacatt attttccaaa ataatttagc aggaatcaca ctagtaaact cagataactc
     1141 tgttatcact ttgaattcta ttactgaatc tggaaatggt ctttatatag acaatcaaag
     1201 taaccaaaac aatatcaact ttaacaatgt tcttttgaac acaatcgatg taaacaatgc
     1261 taatgggtta ccagtaaaca ccaatggcaa tacatatgat caaaacaatt gtatgactgc
     1321 taatccaagc ggtatatgta ttggtaagta atctagacaa aaaaattcct tctctttttt
     1381 tctttatata taattaaaaa gacacaacta gttaagtatt ataattatta aattcaaaca
     1441 tatagttttt ataaataaca aaagaaaacg tgttccaata tcaatagaat aaaataatga
     1501 aatcaaatat aaaataatat aatatataaa aaatatataa ccccctaata aaaaatgata
     1561 aatattgaaa cagtaattgt gtaaattcta attcgattct tttttattat aagactcagg
     1621 aagcctatac aagatatatt tatcctctgg agaaaattta gctggagttg gatcaacagt
     1681 tagagatgaa caatgtgaac atctactctt aagagtataa attcgacaca ttaaacattt
     1741 tctcaaaata tgtttcattt taatgaacta attgttgttg tgttttttta gattcttgac
     1801 gaataaaatt aaagtgacca tcctcctttt caattgaaga ttttattttt tctatagaat
     1861 tgttaataga tttctcagcg attttaaaat tatcagcagc aacaactatt ctatatttag
     1921 gtgctgcaat atatgaaatc ataatcttcg aattaccttt tgtagtttct gcagtactta
     1981 aaatagattt aataatttca actccattgg atttttgcga tgaaatttcc aatatacctc
     2041 ttatttcgac atgggggatg ggaatttttt tactttcatc ttcaattatt ttaattacat
     2101 tttcggttaa ttctagttgt aataatacat ctttcccttt ttttgttgta gcctcaaaaa
     2161 tatcataaac ataatcatat ttagttaaaa ttttttcttc aatt
//
LOCUS       DASVEV010000061         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_16080, whole genome shotgun sequence.
ACCESSION   DASVEV010000061 DASVEV010000000
VERSION     DASVEV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16080"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            923..1570
                     /locus_tag="VF222_03210"
     CDS             923..1570
                     /locus_tag="VF222_03210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7141469.1"
                     /translation="MSYNPSEYWHERGKIYKKNFRYDKNKRLQEEFLISHLNSIPGSF
                     KSVLELGCGFGRITKLLLTNYNTITEYLSVDISPDQIENAKSLITSSKLPNEVKLDFR
                     VSDIQSLKLDKEYDLVILSEVLLHILPTDIDSIIKKLITFSKKHIINIDWYEDEPPKN
                     QALHNFIHQYEAIYKKYTDPSTTIKRIPIIRKKFLGTLDTKQSIFHVIIEKTPTN"
     gene            2387..>2791
                     /locus_tag="VF222_03215"
     CDS             2387..>2791
                     /locus_tag="VF222_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141470.1"
                     /translation="MDKSVTLSLLLMGSLIMLVPFPNMNIFSNAMAIEDYSDKGNNDN
                     NRYQEEDSDKGNNDNNSPKHEEEDSDKGNNDNNSPKYEEDYSDKGNYANNLPKYEEDY
                     SDNYRVDSSQQSTYDNGYGYDDKNKPPEIFPAN"
BASE COUNT         1081 a          356 c          354 g         1000 t
ORIGIN      
        1 attttggcct tataataaat tgttaacagg aaaagtcata gagatgttat tgtataaaga
       61 tgaattaaaa gaaaataaag attatgatga aaaaaagatt ttaaccttat caggattaga
      121 gaagattgaa ttatttataa aatctaaaag catataaaaa attgaatgaa actacctaac
      181 taaaactttt ttataactat attttagtat tcgatataat aaatagatta actgttaccc
      241 taccattaac caaacagagt ttgtaatatc atgcgaggta aattagattt actaacataa
      301 aatataatat tatcatttac aattctttag aaagtattca ttaataacct attatttttt
      361 aaatcatcag ttgtgaaatc atttcttttc aagggtgtgt ttggttctaa tcttatacta
      421 tttctttttc taaagtgttg ataagtaatg tgtgtctgat agatataact taatataata
      481 atgaagggtg tatcattaaa tacatttttt aataaggttt ttttctaaat atcactgaat
      541 cttctccttg tttgattatt gatatttgtc aagattgccc ctgatataat agcactccta
      601 ttctatgatc tgtttatatt aaattattgt ttcttttttt gtctctattt ctccttaaat
      661 ctttttatta cttcttgaaa tattctaatt atagattaaa aattgtaatt atcctgaata
      721 tttaattgtt attagatata tagcaaaggt aagatattac taatgatata aaaataacca
      781 tgaattcatc ttgcttgttt ggaactccct ttcgaagata atagtaaaaa agttgtaaaa
      841 ggtgatttgg ttaatgagtt attattaatc tctacttatg tgagaaatct atttatattt
      901 aagttaattt ataaaattaa aagtgtctta taacccttct gaatattggc atgaacgtgg
      961 aaaaatatat aaaaaaaact ttcgatatga taagaataag cgtttgcaag aagaatttct
     1021 aatatctcat ttaaatagta ttccgggatc atttaaatct gtactagaat taggctgtgg
     1081 atttggtcgt ataacaaaac ttttattgac caactataat actattaccg aatatttatc
     1141 tgtagatatc tccccagatc aaattgaaaa tgcaaaatcc ctcataacat cgagtaaatt
     1201 acctaatgaa gtcaaattag actttcgcgt atctgatatt cagtctttga aattggataa
     1261 agaatatgat ttagttatcc tgtcggaagt tcttttgcat attctaccta ctgacattga
     1321 ctctattata aaaaaattaa taacattctc taaaaaacat attatcaata ttgattggta
     1381 tgaggatgaa cctccaaaaa atcaagcact tcataatttt attcatcaat acgaagccat
     1441 atacaaaaaa tatactgacc cctctacaac aataaaaaga attccaatca tcagaaaaaa
     1501 attccttggg actcttgaca caaaacaatc catatttcat gttattatag aaaaaactcc
     1561 tacgaattaa tgattaaaat ggattatttt aaaaaacaga aaatttttgt aactatttat
     1621 gttttgaaca tttctattac aaattaagat tcgctttaga gagaactgct agagatagaa
     1681 aattcttaat ttccattcat ccatacataa atatgttgaa tttaaatttc ttagttttat
     1741 ccatatgttt cacattattt tttcaatatt tgtagtgcca gaattgaaca gaaataaagg
     1801 aaattaactc ttaaagcatt aataattcat cgctgaaagg taaataaact ttttcatttt
     1861 atttattgac aacttgctag aaaattggtt attgatggga agtaaaattt gaatagttat
     1921 tgctgttata ttcatcaatt ttaaaatttt tttggttagc ttagagaaga gggactaaag
     1981 aataaaaaca atgaataatt aatacttaaa aataaaacaa gaaatgtaag tgtctgataa
     2041 gtctttttat tcatttttta tacatttcta tattttaata aaatttacgt agttcataaa
     2101 agatattaat tttttatatt gaatttgata atttaaaatg attttataaa tgccatagtt
     2161 taggacacag agaaattaat aactgatggt tgattataga agtcatagaa tcattgcaag
     2221 agaaaattcc atagtatata atattatact atttttagaa attgatttac taataatatt
     2281 tagcaaatga ttattgtaag aacaattccc taatctaatt tagtaatact atcttagcaa
     2341 atatacctaa gaactgcaat tatattttac ctgtttacaa actgacatgg acaagtcagt
     2401 aacacttagt ctacttttga tgggctcatt gataatgtta gtaccttttc ctaacatgaa
     2461 tattttttcc aatgcgatgg ctatcgaaga ctattcagac aaaggtaata acgacaacaa
     2521 tagataccaa gaagaagatt cagacaaagg taataacgat aacaattcac caaaacacga
     2581 agaagaagat tcagacaaag gtaataacga taacaattca ccaaaatacg aagaagacta
     2641 ttccgataaa ggtaattacg ccaacaattt accaaaatac gaagaagact attctgataa
     2701 ttatagggta gattcctctc aacaatcaac atatgataat ggttatggat atgatgataa
     2761 gaataaaccc ccagaaatat ttccagctaa c
//
LOCUS       DASVEV010000062         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1182361, whole genome shotgun sequence.
ACCESSION   DASVEV010000062 DASVEV010000000
VERSION     DASVEV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182361"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..>2994)
                     /locus_tag="VF222_03220"
     CDS             complement(92..>2994)
                     /locus_tag="VF222_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HEX7141471.1"
                     /translation="NEQLRFQHIEKDIKKYKAIKISNESRSIRRNIMTNNQELETKRI
                     KYTDVGSELKKTKQELESLDLEKMKFIKEVDLINKEKAEISNRISSIVYDVEKSKAIL
                     KETISRMEQIDKKLRIAEITEMESNVKIKNSQNEIAILEKNSETISKRKDIHTFELKS
                     LDNHILNLTTKTQKIELHKRKINNRYEKILLLNNKFEVNSLKINENLKITLNNKVETN
                     SKVKNLKDKISSISQELKNNINYVIELRIQIEKIHKFILKIEKIIKDFNDDIEESKII
                     LNNSDTEALRFYEKNKILTESMSEDLTISEIIKEKELPIVGIVHDLVKWDKNYHRPVM
                     AVGSEWLKAIVVNNVEDMIKIAEFAKVKKLPRVKIIPLDIIQLSEPVVLYEQNISVIG
                     NLSTFIKSDNKKLIDFIFGNTIVVRTASEAYKLSLEGYRSVSIEGEFFESKSKVLLVD
                     YNSAVINFVTEINLKNDIESLRGLILKLKDSLLKKTTEHRNLLDKLKNLQSEKIGLEN
                     KIKYTEDKVNFQKKILEDERIELKGLEILQSKNESDYKNVIILLKKIKKRFFLIAGTK
                     KKMEESLQKIKLSFEETKIADLNLERSKYQTILESDNQELQNIVIKLSEKKNGRDLLL
                     ERINTLYEERKILTKEQSDKEVWMSDLKDKIEKEEENLNFLRDKEQNIIFSSGDTYTI
                     LQSYEEKIKKLLDHERKLSREYSSLEREIVLLEKETSNLKQYEVKLNNDLMWLGYKEL
                     NETNYDVDNLVIQLSEEYEILKSKINLRADENYIQVVNGYRGMSERKNDLEKERNSIV
                     IFIEEIYKEKKNLFMDAFLKINKDINFIFSTIIGGNAWLEIENEEDIFSKGVRLIVQF
                     PGKPRRDSTALSGGEKTMAATIFLLALQAIKPSPFYLMDEVDAHLDAQNTERLSKILF
                     ERSKNNQIILVTLKDSTIAKVDQIYGVFPREGVSHIVKYKYHKKELDEIPLT"
BASE COUNT          860 a          493 c          311 g         1330 t
ORIGIN      
        1 aactgagcca gaattaattg aagcaaatca ttcacatttt attcggtgtt ataattatga
       61 atccatttga cctaccttta tttctaatcg tttaagttaa agggatttca tctaattcct
      121 ttttatgata tttatacttg acaatgtgag aaactccttc tcttggaaag actccgtaaa
      181 tctgatctac ctttgctata gtagaatctt ttaatgttac cagaattatc tggttatttt
      241 tagatcgttc aaataatatc ttagatagtc tctcagtatt ttgtgcatct aagtgtgcat
      301 ctacctcatc cattaagtag aatggagacg gtttaattgc ttgaagggca aggaggaaaa
      361 tagttgctgc cattgtcttt tccccacctg ataatgcggt agaatctctt cttggctttc
      421 ctggaaattg aactattaac ctaactccct ttgaaaaaat atcttcttca ttctcaattt
      481 ctagccaagc gttgccgcca ataatagtag aaaaaataaa gtttatatct ttattaatct
      541 ttaagaaagc atccataaaa agattctttt tctctttata tatttcttca atgaaaataa
      601 cgattgaatt tcgttccttt tctaaatcat ttttcctctc agacattcct ctatatccat
      661 taacaacttg aatataattt tcatcagctc ttaaatttat tttacttttt aatatttcat
      721 attcttcaga taattgaatt acaagattat cgacgtcata gttagtttca tttaattctt
      781 tatatcctag ccacataagg tcgttattta atttaacttc atattgtttc aaattcgaag
      841 tttctttttc taataaaaca atttctcttt cgagggaact atattctcgg gaaagtttcc
      901 tttcatggtc taacaatttt ttaatttttt cttcatatga ttgtaatatt gtatatgtat
      961 ctccagatga gaaaataata ttctgttctt tatctcgtaa aaaatttaaa ttttcttctt
     1021 ctttttctat tttatctttt aagtctgaca tccaaacctc tttgtcagac tgttcttttg
     1081 tcaaaatctt cctttcttca tataaagtgt taattctttc taagagtaaa tctcttccat
     1141 tttttttctc cgaaagttta atcacaatat tttgtaattc ctgattatct gattcaagta
     1201 tagtttgata tttgcttctt tctaaattta aatcagctat ttttgtttct tcaaaactta
     1261 attttatttt ctgcaggctc tcttccattt tttttttggt accagcaatt aaaaaaaatc
     1321 tttttttaat tttttttagc aatattatga catttttata atcactttca ttcttacttt
     1381 gtaaaatctc taatcccttc aattctattc tctcatcctc taatattttt ttttgaaaat
     1441 taactttatc ttctgtatat ttgattttat tttctaaccc tattttttca gattgtaaat
     1501 tttttaattt atctagtaaa ttgcgatgct ctgttgtctt tttcaaaagt gaatccttta
     1561 attttaatat tagtcctctt agactttcaa tatcgttttt aagattgatt tcagtgacaa
     1621 aattaataac tgcagaatta taatctacta aaagtacttt tgacttggac tcgaaaaatt
     1681 ccccctcgat tgatactgat cgataccctt ctaatgagag cttgtaagct tcgcttgctg
     1741 ttctaacaac aatggtattt ccgaaaataa aatcaattaa ttttttatta tcagacttga
     1801 taaaagtaga taagttacct ataacggata tattctgttc atataataca actggttccg
     1861 aaagttgtat gatgtctaag ggaattatct ttaccctagg taattttttc actttagcaa
     1921 attctgcaat ttttatcata tcttctacgt tattaacaac tattgctttc aaccattcag
     1981 atccaacagc cataaccggc ctatgataat tcttatccca tttcactaaa tcatgaacta
     2041 tacccacaat aggtaattct ttttccttaa ttatttccga aatagttaaa tcttcagaca
     2101 tagattcagt taaaatctta tttttttcat aaaatcttaa agcttcggta tctgaattat
     2161 tcaatattat tttagactct tctatatcat cattaaaatc ttttattatt ttttcaattt
     2221 tcaatataaa tttatgaatt ttttctatct gtattctcaa ttcaatcaca tagttaatat
     2281 tattttttaa ttcttgagaa attgatgata tcttatcctt caagttcttt actttagaat
     2341 ttgtttccac cttattgttt aaagtaattt tcaaattttc atttattttt agactattaa
     2401 cttcaaattt gttgttcaag agcaggattt tttcgtatcg attatttatc ttccttttat
     2461 gtaattctat tttctgggtc ttagtagtta ggtttaatat atgattatct aaacttttta
     2521 actcaaaggt atgaatatct ttccttttgc ttatcgtctc gctatttttt tctaaaattg
     2581 caatttcatt ctgagaattc ttaattttta catttgattc catttctgta atctctgcaa
     2641 ttcttaattt tttatcaatt tgctccattc ttgaaattgt ttctttcaaa atagcctttg
     2701 atttctctac atcatatacg attgatgata tcctattact gatctctgct ttttccttat
     2761 tgattaggtc tacttcttta ataaatttca ttttctctaa atctaaactt tctaactctt
     2821 gttttgtttt tttcagttct gagccaacat cagtatattt tattcttttt gtttctaatt
     2881 cttggttatt agtcataata tttcttctaa tacttctact ttcatttgaa atttttatgg
     2941 ccttgtattt tttgatatcc ttctctatat gctgaaatct taattgttca tttc
//
LOCUS       DASVEV010000063         8030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1377228, whole genome shotgun sequence.
ACCESSION   DASVEV010000063 DASVEV010000000
VERSION     DASVEV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377228"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF222_03225"
     CDS             <1..431
                     /locus_tag="VF222_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141472.1"
                     /translation="REIPRYTQRIAEYQEQTIQTVKDIASDFIEAEKEVIGSFQSQVD
                     RNSNGVWDLYNPQRIAENHAVMVNNFTSYLLNTANLINNALASNMRVYNTALEQTRDN
                     LKAFAKTNTNYIKAVDSQNREVNTNSSSNNNNSNERTTRL"
     gene            1079..1276
                     /locus_tag="VF222_03230"
     CDS             1079..1276
                     /locus_tag="VF222_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HEX7141473.1"
                     /translation="MESIKRKDIKPGTIVKIIEKQHQKTGELTEGIVKKILTSSATHP
                     YGIKVILESGIVGRVKEIVNK"
     gene            1506..2189
                     /locus_tag="VF222_03235"
     CDS             1506..2189
                     /locus_tag="VF222_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7141474.1"
                     /translation="MFQDFNLENLILLNQSIAKCSKCVRLSEYIKNVGTKKVKRFKNI
                     NYWSKPVCGFGDINADLIIIGLAPAAHGGNRTGRMFTGDSSGDWLVKALYEYNFCNQP
                     FSNDTNDGLILNNVYITSAVKCAPPKNKPLLVEISNCNEYLIKELKLLSKTSKIFLTL
                     GKLAFDEFCKIHKIKSIKFRHGLIYHIGNSNKILISSYHPSRQNTNTGKLTWSMWIDI
                     FRQISDILD"
     gene            complement(2446..4464)
                     /locus_tag="VF222_03240"
     CDS             complement(2446..4464)
                     /locus_tag="VF222_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX7141475.1"
                     /translation="MVEPLERPLSSYIHTQYVTADENSSVAEAVKILQPKNVEIILVT
                     SSSNGKYVGIVTDSDILEKVVMKGEDSDLVFLKSIMTSPIITLPSSSTVRQAIKVMRS
                     HKIKHLPVTDSTNNNNEEGKIIGTVTQEYLAEVIRIAVIEKTFRSYRKIIREHYKPIF
                     ANVAIIMQFTGLLMVVPALLGTIWGEWQSSIGIYLAFVGMSLTGYVMNTLGEKSPLNL
                     KQSSIVVVLSFVLLSLFGSLPYMYINPFWEGIDFFSLFASSFLESASGFTTTGLSTII
                     HPENLPDSFSFYRSYTEWVGGLSFVYLVMALYYPETKLAGMKYFLGSGILRFKQLLST
                     ISIIFVVYTTIFVLLIYIFGHINILDSISLAFTTLATGGFVPTSTILTSENSITLAII
                     MAGMIIAALPFAFHFGIFSKNVEATKEIKEILIFLIIMTFFIFLFILIEPSFSENDLL
                     SSVFHVISASTTTGFQFLNLSHLSINGKILLIVVMLIGGTAFSTAGGIKIARLLLIFE
                     KLINNKKYISTFNNKNHNNSQQPPPGISATAIQFRKKIQQTFKSYYQEKKLKNNKQIS
                     SIVPLDKYKFNILSDKAFKEALFVIVLYILFTVVTALCIYYLSNNSIIFIDAVFEAAT
                     TISNTGLTVGITNMDLDSISKMILSFNMIMGRFEIIAILYIFISKLRK"
     gene            4821..5744
                     /locus_tag="VF222_03245"
     CDS             4821..5744
                     /locus_tag="VF222_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class oxidoreductase"
                     /protein_id="HEX7141476.1"
                     /translation="MFKPKHLTLGVLFAIEAYEGSIPKMERQVELAQRAEELEFSGLW
                     FRDVPLYDPTFGDVGQIYDPWTYLGYIAGQTKNIPLGTAAIIFPLRHPIDLAKGASSI
                     DQLSNGRLILGIAAGDRPIEYPAYGRNFERRDILFRESVYDFHRLLSENFPRYNSIFG
                     SLTGEGDLLPKLTSKKLPLFVTGYSRQTIEWIAENSDGWLMYPRDTLLQQHIVTNWKN
                     VVRRTGNDFKPFAQSLYIDLTKDPDTNPDRIHLGYRLGRNNLIKYLNDLKDIGVNHVF
                     FNLKYSKRPADDVLEELGKFVIPYFPPLDIQ"
     gene            complement(6078..6641)
                     /locus_tag="VF222_03250"
     CDS             complement(6078..6641)
                     /locus_tag="VF222_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141477.1"
                     /translation="MILLFTSPIIIINTNEMSLTAGIAQKEKIDSDNSVYAQDTFSAI
                     GQISSLVITLPESNFNITNVFKVILTGEWTLSVNKGNVTNFSTNFLASPMDGSKSHIH
                     QITNFKLENGSQKTIVQLTPGNSLTVNGTADIKINGQTIWNNADISISISKGSTIAID
                     PDDEDTNNHFGEQQVYGIITKLIDISN"
     gene            7021..7341
                     /locus_tag="VF222_03255"
     CDS             7021..7341
                     /locus_tag="VF222_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141478.1"
                     /translation="MAYVSDNPNFTEKSENDGSRLGDIEGKSAENSPIDIKKASKLAN
                     LLEGISFPANKEKIKDHLNRLSPSMGNRVNDVLEAIQNNLDENVEFNSTYEIEKAAGL
                     VREL"
     gene            7737..>8030
                     /locus_tag="VF222_03260"
     CDS             7737..>8030
                     /locus_tag="VF222_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141479.1"
                     /translation="MSVKEEKINDKNTPKFSTDNTNTTNFNERINRDYNNTLNQQQDA
                     INKTLDNTLNNVKRTTDEATREIPRYTQRIAEYQEQTIQTVKDIASDFIEAEKE"
BASE COUNT         2957 a         1097 c         1157 g         2819 t
ORIGIN      
        1 cacgagagat tccacgatac acacaaagga ttgctgaata tcaagaacaa actattcaaa
       61 ctgtaaaaga tattgcttct gactttattg aagcagaaaa agaagtaata ggctcttttc
      121 aatcacaagt agatagaaat agtaatggtg tatgggattt gtataatcca caaagaatcg
      181 cagaaaatca tgcggtaatg gtaaataact ttacaagtta cctgttaaat actgccaatc
      241 taataaacaa tgctctagca tcaaatatga gagtatacaa tacagcacta gaacaaacac
      301 gtgataatct gaaggctttt gcaaaaacaa acaccaatta cataaaagca gttgatagcc
      361 aaaatagaga ggtaaatact aacagcagca gcaacaacaa caatagcaac gaaagaacaa
      421 ctagacttta ataaattttt aaacctagtc cgataatctt ttgtattttt taggttattt
      481 tttaaattat ctatttagtt ggtctcttat attatattag ctaattaaat attctccttt
      541 ttaagattct aacttaagaa cattatagac ctgaatatat tcaacatctg aaattttatg
      601 gtatgtctaa tccatttttc attgtctttt aaattccccc ttggttataa aaatccttta
      661 aatttttcat tctctccaga ataatctaat atatattcaa atattctagg attgtctttg
      721 ctaactgatg tttttcagtt tataaaagtt ctgtaagcac ttgttttatt tttaattttt
      781 atttactttg aacaaaccat caatatagta atatttgcta ctagtcctga ggttgatcct
      841 ttatgttgtt agttctctta tccgcttgtc tatcaaggaa atatatggaa agatatagtg
      901 gaaatccaag gaactctttt gaaagagtat gacaataata ataataacta ctctaaattc
      961 aggcatgtgt atgtatatgt tgttcttact cataactttt cgaacaatat tattaagcat
     1021 tttaaagctt attgttggtt aacaatttat ataaaatttc ataaataatt aaaagatatt
     1081 ggagagtata aagaggaagg atataaaacc aggcactatc gttaaaataa tagaaaaaca
     1141 acatcaaaag actggagaat tgaccgaagg aatagtaaag aaaatcttaa cctcttcagc
     1201 aactcaccca tatggaatta aggttattct agagagtggg atagttggaa gagttaaaga
     1261 gattgtaaat aaataggatt aaagaaaaat tctcaaaagg cttctgagat ggtaatctct
     1321 tgttatatgt catcgttatc tttttcaaac tttattctac tttcatttat ctatcgattc
     1381 actttcaatt ttaaagtaat ccaattaaag ctaaaaattt cctatatatt tataatttgg
     1441 caaaagagga atagtagtag aaagtttacc ataacattat attttacaaa tatattgtat
     1501 ttctattgtt tcaggatttt aatttagaaa atttaattct tctaaatcaa agtattgcga
     1561 aatgcagtaa atgtgtaaga ttatcagaat acatcaaaaa tgtgggaact aaaaaagtta
     1621 aaagattcaa aaatataaat tattggtcca aacctgtatg tggttttgga gatataaatg
     1681 ctgatttaat cataatcggt ctagctcccg ctgcacatgg aggaaataga acaggaagaa
     1741 tgtttactgg agatagttct ggagattggc ttgtgaaggc tttatatgaa tataattttt
     1801 gtaatcaacc ttttagtaat gatacaaatg atgggttaat attaaataat gtatatataa
     1861 cctctgcagt aaaatgtgct ccaccaaaaa acaaacctct attagtagaa attagtaatt
     1921 gtaatgaata tttaattaag gaattaaaac tactaagtaa aactagtaaa atatttttaa
     1981 ctttaggaaa attagctttt gatgaatttt gtaagattca taaaataaaa agtataaagt
     2041 ttagacatgg attgatttat catataggta atagtaataa gatattaata tcctcatatc
     2101 atcctagtag acaaaatacc aatacaggaa aattaacatg gtccatgtgg attgatattt
     2161 ttagacaaat aagcgatatt ctcgattgat atagaagata aatttgatat atattaaaca
     2221 ttgttggcaa agaatattca ttatatagta caaacaaaaa atcattcctt tacattaact
     2281 aaaagttgag tagttgttac ttgtctgtcc attgttgaaa tatataatta gtactctaac
     2341 atttcctaat ttattctaat ttaacctaca atatttcaag aatataattt ctaatttaaa
     2401 ttataaatga atacaactat tacttcatat tacatttaac aacaactatt ttctcaactt
     2461 tgagataaaa atatagagta tagctattat ttcaaatctt cccattatca tattaaaaga
     2521 tagaatcatt ttagaaattg aatccaaatc catatttgtt attcctactg ttaggccagt
     2581 atttgaaatg gtagtagctg cttcaaatac tgcatcaata aaaataatac tattattact
     2641 caagtaataa atacataatg cagttacgac tgtaaacaaa atataaagaa caataacaaa
     2701 taatgcttct ttaaatgctt tatcagaaag aatattaaat ttgtacttat ctagaggaac
     2761 tatactactt atttgtttat tattttttaa ttttttttct tgataatatg atttgaaagt
     2821 ttgctgaatt tttttacgaa attgaattgc agttgctgaa attcctggag gaggctgctg
     2881 actattatta tgatttttat tgttaaacgt ggagatatat tttttattgt taatcaattt
     2941 ttcaaaaatt agtaataatc tggctatttt tattccacca gcagtagaaa atgcggtgcc
     3001 tcctattagc ataactacaa ttagtaatat tttaccatta atagatagat gagataaatt
     3061 aagaaattgg aatccggtag ttgttgaagc gctaattaca tgaaatacag aagaaagcaa
     3121 atcattttct gaaaatgaag gttctataag tataaataaa aagatgaaga aagtcataat
     3181 tattagaaat attagaattt ccttaatttc ctttgttgcc tctacgtttt tactgaaaat
     3241 accaaaatga aatgcaaaag gtaatgctgc aattatcatt ccagccataa ttatagccaa
     3301 tgtaatcgaa ttttctgaag taaggatagt agaagtaggt acaaatccac cggttgctaa
     3361 tgtagtaaag gctaacgata ttgaatctag aatatttata tgtccaaaaa tatatattaa
     3421 taaaacaaaa attgttgtgt aaactacaaa gataatacta atagtagaaa gtaattgttt
     3481 gaatcttaat attccactac ctagaaaata tttcattcct gctaattttg tttctggata
     3541 gtacaatgcc ataactaaat aaacaaaact taatccaccg acccattctg tgtaggagcg
     3601 gtaaaatgaa aaactatcag gcaaattttc aggatgtatt attgtagaaa gacctgtagt
     3661 tgtaaaccca gatgcacttt caaggaacga acttgcaaaa agagaaaaga aatcaattcc
     3721 ttcccaaaat ggattgatat acatgtatgg tagactacca aataaactca acaatacaaa
     3781 acttaataca acaacaatag atgattgttt tagattgagt gggctttttt ctcctaatgt
     3841 attcataaca tatcctgtta aagacattcc tacaaatgcc aagtaaatcc caattgaaga
     3901 ttgccattct ccccaaattg tgcctaataa tgcaggtaca accattagta atcctgtaaa
     3961 ttgcattata atagctacat ttgcaaaaat tggtttgtaa tgctctctta taattttcct
     4021 ataacttcta aaggttttct caataactgc gatacgaatt acttctgcaa gatattcttg
     4081 tgttactgta ccaataattt tcccttcttc attattatta tttgtactat cagtaacagg
     4141 gagatgtttt attttgtgag aacgcattac tttaattgct tgtcttactg ttgacgaact
     4201 aggtaatgtt attatgggag atgtcattat ggatttgaga aatactaaat cagaatcctc
     4261 tcctttcata acaacctttt ctaaaatatc actatctgtt acaattccaa catattttcc
     4321 atttgatgat gatgtaacta gaataatctc aacattttta ggttgcaata ttttaactgc
     4381 ttcagcaaca gaagaatttt catcagcagt gacatattga gtatgaatat acgatgaaag
     4441 gggacgttct aatggttcaa ccattaacaa tcatcattta taattttcct gtttgctttg
     4501 agctcataaa ttacttaaat gttattttaa ttagatgcca ttttaataaa aagtataata
     4561 aataaagaat ttgaacattt actgtgaaat attatacgta tctttttata attgggatca
     4621 acatattgca tttttagaat caaacgttaa cttacgtaaa atatcaatgt taccataagt
     4681 aaaatcattt ttaaacttta attcatggaa ttatgataaa aaggagttgt tttagtaaac
     4741 atgaccttta aggtttcttt ttatagtttt atttaattat tataacaaca atgtcttttt
     4801 ttaatagagc ttataaatta atgtttaaac caaaacacct tactttagga gttttatttg
     4861 ctattgaagc ttatgaaggt tcgattccaa aaatggagag acaggtggag ttagctcaac
     4921 gtgctgaaga attagaattc tcaggattat ggtttcgtga cgtcccttta tatgatccta
     4981 cttttggaga tgtaggacaa atatatgatc catggactta cttgggatat attgctggac
     5041 aaacaaaaaa tattccatta gggactgcag cgattatttt tccgttaaga catccaattg
     5101 acttggcaaa aggagcatct tcaattgatc aattaagcaa tggtcgacta atattgggaa
     5161 tagcagctgg ggataggcct attgagtatc ctgcatatgg acgaaatttt gagcgaagag
     5221 atatactttt tagagaatct gtttatgatt ttcatcgttt gctttctgaa aattttccaa
     5281 gatacaattc aatttttggc tctttgactg gcgaaggcga tcttttacca aaacttacat
     5341 ctaaaaaact gccattattt gttacaggat atagtaggca aactattgaa tggattgctg
     5401 aaaattcaga tggatggttg atgtatccaa gagacaccct tttacagcaa catattgtaa
     5461 ctaattggaa aaacgtagtt agaagaactg gaaatgattt taaacccttt gctcagtctt
     5521 tatatataga cttgactaaa gatccggata caaatcctga tcgtattcat ttaggatatc
     5581 gattgggaag aaacaatttg attaaatatt tgaatgatct aaaagacatt ggagtgaatc
     5641 atgtcttttt taatcttaaa tatagtaaac gccccgcaga tgatgtcttg gaagaattag
     5701 gaaaatttgt aattccttat tttcctccgc tagatataca ataataatct acccattaac
     5761 tcaacaataa tttttttaca tttcaagata cctctctatc aaattgtcat gtctattcat
     5821 agtatacgta tgatgtggaa aaagttttgt tttatatttc atttgaatat acatattata
     5881 gtatatattt aatatgttta ttgtctatag atatatctga tatagtattg ttttgataaa
     5941 caatatattt atcttttaaa attcacttta agtatcaaaa ggattttctt tttattctaa
     6001 aataatacaa tacaatagta tcttcaaaaa ttttcatgta tttttctaaa ttatatttat
     6061 ttttattttt actgaaacta attactaata tctattaatt tagttattat accataaacc
     6121 tgttgctctc caaaatgatt attggtatct tcatcatctg gatcaattgc aattgtactt
     6181 cctttagaaa tagatattga tatatcagca ttattccata tagtttggcc attaattttt
     6241 atgtcagcag ttccatttac tgttaagctg ttacctggcg ttagttgaac tatcgttttt
     6301 tgactaccat tctcaagttt aaaatttgtt atttgatgaa tatgactctt tgagccatcc
     6361 ataggagatg caagaaagtt tgttgagaaa ttagttacat ttcctttatt cacacttaaa
     6421 gtccattctc ctgttaaaat tactttaaat acatttgtaa tgttaaagtt agattctggt
     6481 agtgtaataa ccaaggaact tatttgtcct atagcagaaa aggtatcttg agcatatacg
     6541 gagttgtcag aatcgatttt ttctttctgt gcaattccgg ctgtcagtga catttcatta
     6601 gtattaataa taataatagg tgatgtgaaa agtaatatca aaatataaat aaacatatat
     6661 ttcacaattg aaatttaaaa ttatctcacg tattattatt agttttctct tttaaccttg
     6721 tattctcata ttacagatta gggcttatac gagtagcggc aggggttgaa ggccaggcat
     6781 gcaatttttt tcaatagtta taactcattt gtttaagaag gttaaaacta taaaacgttt
     6841 tatcctaatc ttttactttg tattgttttt acttttttca ctcatgtttt gatttttcgt
     6901 actactgtta taatagaaca taaattatat tactaatagt tgttttatag tataatgtcc
     6961 ctattgtaat aatgacttac cttgcatctt taataactaa taataggagg aggaaattaa
     7021 atggcatatg tttctgataa tccaaatttt accgaaaaaa gtgaaaatga tggttctaga
     7081 ttaggagata ttgagggaaa atctgcagaa aattctccta tcgatataaa aaaggcatct
     7141 aaacttgcaa acctacttga aggaatatct tttcctgcaa ataaggaaaa gataaaggat
     7201 catctaaata gattatctcc ttctatggga aatagggtaa atgatgtttt ggaagctatt
     7261 cagaataatt tagatgaaaa tgttgaattt aatagcacat atgaaataga aaaagctgct
     7321 ggactggtta gggagctgta ggaaaaaaac tatttgaata ttgtaactat aacaaatata
     7381 atatgaaatt tattttcaat acttacgtga tttctattag tatttaaaaa ataaagaata
     7441 aaaattaagg aattgtcaaa ccattagcaa aatcaatcaa atatttcact attaattctt
     7501 tggtagatgt tatcataatt ctgcaacaga ggaaggttaa agctaataga tctaatatta
     7561 aggaattctc ctctacagaa attttacccc atactgcgat acttcaatta tggatttaat
     7621 gatggagctt gagtgaataa tgaccagcag attgaccaga attatatcta cagaatatga
     7681 ttatccacta gatattctta cctaagtata aatacattaa taactttagg gtaattatgt
     7741 cagtaaaaga agaaaaaata aatgataaaa atacacctaa atttagcaca gacaacacca
     7801 ataccactaa tttcaatgaa agaataaata gagactacaa taacacctta aatcaacaac
     7861 aagatgcaat aaacaagaca ctagacaata ccttaaataa tgtaaaaaga actactgatg
     7921 aagctacacg agagattcca cgatacacac aaaggattgc tgaatatcaa gaacaaacta
     7981 ttcaaactgt aaaagatatt gcttctgact ttattgaagc agaaaaagaa
//
LOCUS       DASVEV010000064         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1182767, whole genome shotgun sequence.
ACCESSION   DASVEV010000064 DASVEV010000000
VERSION     DASVEV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182767"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..788
                     /locus_tag="VF222_03265"
     CDS             255..788
                     /locus_tag="VF222_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141480.1"
                     /translation="MNNFNIIKQFNKNFIVFFVLLSLSSIYLFQSFSLENKNILIKEA
                     YGIIQTFPMEIDYAHFISPLNQDNQIKVLLKSLTTSDAAGLNNNKTSDAAGLNNKTIN
                     AVMKVYSINGTLLKTSSYPQGFNYNNTESIKLATNIADKSIETVTAVIQLTNLEKTQQ
                     LSDPLTIKLGLGQVIDR"
     gene            873..1538
                     /locus_tag="VF222_03270"
     CDS             873..1538
                     /locus_tag="VF222_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HEX7141481.1"
                     /translation="MGIYKYAFYKSPNIGIFVKCNDNFIVLPLGFAPTKTNRLSQYLE
                     VTDTIYVSIADTRLLGPMMVINNNGILVSYITSDEEINILKEKSQLNVERLKSKYTAI
                     GNLIATNDHGAIVSPLCNDVKTQIQDILGVSVTSMSISGFIQTGAMIVATNKGAVVYP
                     NASDDEIKTISNILNVNVEPVTINGGIPYLASGIIANSKSVVVGNMTSGPELIMLSRA
                     FNV"
     gene            1631..1764
                     /locus_tag="VF222_03275"
     tRNA            join(1631..1663,1691..1697,1720..1764)
                     /locus_tag="VF222_03275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Leu,seq:cag)
     gene            1981..2107
                     /locus_tag="VF222_03280"
     tRNA            join(1981..1999,2016..2050,2087..2107)
                     /locus_tag="VF222_03280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2032..2034,aa:Met,seq:cat)
     gene            complement(2105..>2972)
                     /locus_tag="VF222_03285"
     CDS             complement(2105..>2972)
                     /locus_tag="VF222_03285"
                     /inference="COORDINATES: protein motif:HMM:NF020078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HEX7141482.1"
                     /translation="YRKIKNCGTLRIIDPNNVIRLHDTTFPSTYNSYDYIHKRVLILP
                     HGPDPVFCGIRGEEPQVLHNSLKILEIKEDLAGYMIYRSNQGTGMHLQNELKLSTIKS
                     FTSGYSACVIVTNPQIIKGGHVLFTVEDKLKNVCLAAVYEPTGLTKIASQLHIGDQIE
                     IGFGVRKATSIHPTKVLNIEYIFIQQLTQIYNTINPVCINCKKRMKSEGKNKGYQCKK
                     CKIINKNCKKVLLLKTRSIKEGLYIPTLKAHRHLTKPFHRYGKEKTKFFEFGNVELYS
                     KWFKDMDNQKNR"
BASE COUNT         1039 a          419 c          437 g         1077 t
ORIGIN      
        1 cataaattta tggtagaatt tactgatatg aaaggtcaag aacttgtgga tatcaaagaa
       61 acagaagaca aacttgaatt attttttaaa aataataaat atattattca aattattgac
      121 ggaaacttca aaataacaca atcaacataa taatatttgt gaatcttatt tttcactatt
      181 acaatcatat agtattttgt caaattaatt taacatcaat gttattctaa agatattaat
      241 ccaagtaatg gtctatgaac aatttcaata taataaaaca atttaataaa aattttattg
      301 tattttttgt attattatcc ctttcatcaa tttatctctt tcaatcattt tccttagaga
      361 ataaaaatat tttaataaaa gaagcttatg gtattataca aacatttcct atggagatag
      421 attatgctca ttttatatct cctttaaatc aagataatca gataaaagtg cttctaaaat
      481 ctctcactac aagtgatgct gctggattga ataataataa aacaagtgat gctgctggat
      541 tgaataataa aacaattaac gctgtaatga aagtatactc tataaacggt acgttgctta
      601 aaacatcctc ctacccacag ggttttaatt ataataatac ggaatctata aaacttgcta
      661 ctaatattgc agataaatct attgaaacag ttacagcagt tatacaatta actaatttag
      721 aaaaaacaca gcagttatcc gatccattaa caatcaaact tggtcttggg caagttatag
      781 atagatgata gatgaacttc attctctaat tttttatatg tattataata ttccaataag
      841 ttcatattaa tatttataat aattgttaga tcttgggaat ttacaaatat gcattttata
      901 aaagtccaaa tattggaatt tttgtaaaat gtaatgataa ttttatagta cttccattag
      961 gatttgctcc aacaaaaact aataggttat cacaatattt agaagttaca gatactattt
     1021 atgtttcaat tgctgacaca cgattattag ggccaatgat ggttatcaat aataatggaa
     1081 tactagtttc ttacattact tcagacgaag agattaatat attaaaagaa aaaagtcaat
     1141 taaatgttga acgcttgaaa agtaagtata ctgcaatagg taatttaata gctacaaacg
     1201 atcacggagc aatagtgtca ccgttatgta atgatgtaaa aactcaaata caagatattt
     1261 taggggtatc cgttacctct atgagtattt caggattcat tcaaacagga gcaatgatag
     1321 ttgccacaaa taagggtgct gtagtttatc ctaatgcatc agatgatgaa attaagacaa
     1381 tttctaatat tttaaatgtt aatgttgaac cagtaacaat taacggtggg atcccttatt
     1441 tggcatcagg tattattgct aattccaagt cagtggtagt cggaaatatg accagtggtc
     1501 cagagttaat aatgttaagt agagcattta atgtttaggc agattaatat ttaattattt
     1561 cttattcatt ttatgaagga ttattataat gaaaaattta ttatttcact aaggatttca
     1621 tatatagaaa gcgggagttg ccaagcatgg tcaaaggcgt gagatcagat cagttttaat
     1681 ctgacacgac gctcagaagt tttagcattt gactaagata cctcatctct taggggttcg
     1741 tgggttcaaa tcccacctcc cgcatcgcta tagaagcctg gtaagttttt ctaatgattt
     1801 actgtacaaa cttttttatt taggtgttct aatatagaac aaaacattat aaataattaa
     1861 attaataaaa tttttattat aaatattaca tattattaaa tagtatatta aagaattatc
     1921 gaaaaatatt ttaaatttca atagacagag atatatagtt gtaaaatcaa aaaaggaatc
     1981 agcggggtgg ggaagccaga tatataatag gcaaagttat cccggcgggc tcataacccg
     2041 cagagcggtt tctaccaacg tgaatattta tacacggtag ccgtaagttc aaatcaaccc
     2101 cccgctatct gtttttttgg ttatccatat ctttaaacca ttttgaatac aattctacat
     2161 ttccaaattc aaaaaattta gtcttttctt ttccatatct gtgaaaaggt ttggttaaat
     2221 gtctatgagc tttcaaggta ggaatgtata atccttcctt tattgatctt gtttttaata
     2281 agagtacttt tttacagttt ttattgatta ttttgcattt tttgcattgg taccccttat
     2341 tttttccttc tgatttcatt ctctttttgc aatttataca aactggatta atagtattat
     2401 aaatttgtgt gagttgttgg ataaaaatat attcaatatt taataccttt gttggatgta
     2461 ttgatgtagc ttttctaaca ccaaatccga tctctatttg atctcctata tgtaattgtg
     2521 aagcaatctt agttaatcca gttggttcat atactgctgc caaacaaaca ttttttaatt
     2581 tgtcttcaac ggtaaataat acatgtccac ctttaataat ttgtggattt gttactatta
     2641 cacaggcaga atatccagaa gtgaaagatt ttatcgtgga caatttcaat tcattctgta
     2701 aatgcattcc agttccttga ttagaacgat aaatcatata ccctgctaga tcttctttga
     2761 tttccagaat ttttaaacta ttatgtaata cttgaggctc ttctcctcta attccacaaa
     2821 atactggatc aggtccatgt ggtagtataa gaactcgttt atgtatgtaa tcataactgt
     2881 tatatgtaga ggggaatgtt gtgtcgtgga gccgaataac attgtttgga tcaattattc
     2941 taagagttcc gcaattttta atttttctat at
//
LOCUS       DASVEV010000065         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_794857, whole genome shotgun sequence.
ACCESSION   DASVEV010000065 DASVEV010000000
VERSION     DASVEV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794857"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..819)
                     /locus_tag="VF222_03290"
     CDS             complement(400..819)
                     /locus_tag="VF222_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141483.1"
                     /translation="MTLKYFLIGLISISIFFATASTVSPAAFAQNLTIFSLFVNETGG
                     ISGTEHILEGTNEELTNQYYKLGEEFDKKTGIKPGDPVTFVFKNNSAVTIPYPGKYKN
                     VVAEDFLREVIPLGGDRDFICWYYSSTQQTHWVCGHW"
     gene            complement(1203..2315)
                     /locus_tag="VF222_03295"
     CDS             complement(1203..2315)
                     /locus_tag="VF222_03295"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX7141484.1"
                     /translation="MQVTISLSLISIIMIFLVVQPSIEIFSQGQDNTDSYEDLYTKQY
                     NQKDLTEMTIQNYYLKEIIKNRQITNQLFEDKEDEIANTTTPFQLKPGKKIGFVMPTF
                     TMAAYDNYKSDSFYTFYKRYAGISENEFVTTDLDLLTANVPNMTEAFLNQKSDGLQYL
                     AKRISSLSPNMEISFLTDTDIHNGLVFDNNNQNLYDFIILGHQEYVTEEEYYNLKEYV
                     ENGGILILFDSNVFYTEVAYNPIDEEITLIQGHTWAFDGEKAWASVLERWKTETAEWV
                     GSNFFNCGQGCYIDFNNNPFSYVQHEEQHITNPNVNILIDYEAQSIYNILVAAYELDY
                     GKGKVISFGIFGDDIILNKEFLKFFDELFFSHVEAV"
     gene            2779..3147
                     /locus_tag="VF222_03300"
     CDS             2779..3147
                     /locus_tag="VF222_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7141485.1"
                     /translation="MSTTTKLQKKSLNSPDEVRTFEKGKIEIGNIGDASIGRSTFEPG
                     WSWESCVKPIVKTNSCEAPHTGYIVSGRIKVVMDDGTEGEAGPGDIAVIPPGHNAWVV
                     GNEPCVMIDFTGAKDYAKNS"
BASE COUNT         1111 a          456 c          460 g         1130 t
ORIGIN      
        1 aatacaactg atggaatcta cgataactca aactatataa tgtgaatgtt acggagaaaa
       61 ggcatcattt aattaataag gcaaaggttc aattttgtat cttattaaaa ttttgtattt
      121 gtcatattat caattcaatt ctgtgggctc tattagccaa atgggttcta taactaaatc
      181 caaagcttag agggcttcga gttctcatta taacagggtt cggtaacata atagataaag
      241 tttatgatta aaaattttta aataaaattt taatttggat ggatttaatt ggataaattt
      301 ctaataaagt ttttttatag aaaataaaaa aagataaatt ggaaaatcct cattattatt
      361 tttaatcttg ataatccatt tgttatagaa tctaccttct taccagtgtc cacagaccca
      421 atgagtttgc tgcgttgaac tataatacca acatataaaa tctctatctc cgccaagggg
      481 aataacttct cttagaaaat cttctgctac gacattctta tactttcctg gatagggtat
      541 ggtcacggca gaattatttt taaacacgaa tgttactgga tcaccaggct ttatacctgt
      601 tttcttatca aattcctcac ctaacttata atattgatta gttaattcct cgttagttcc
      661 ttctaatatg tgttcagtac cactaatgcc ccccgtttca tttacaaaga gactaaagat
      721 tgttaaattt tgagcgaaag cggcaggaga cacggtagat gctgtagcaa aaaagataga
      781 aatagaaatt agtccaatca gaaaatattt taatgtcatt ttataaaaag gatagcaaac
      841 aaatatttaa atttatttct ctatatagaa atttattgta ttgaatataa ttttgcaaaa
      901 atatatgtta tttgatattc ctgactataa tatgatttta tagcaatagg ttactttatt
      961 tattagtcat aaattaaaac cagttcatat aaattactta ataatttata taaatttgta
     1021 taatctctgt taatacttaa ttgaaatatt gtatatagat ccatataata ttataaataa
     1081 attaattaaa atatttgata tctattgtag gatctgataa atatctcgtt aaatctcttt
     1141 ttcttgatct aaataatact ttattgtagt tattcttaat gtatgataaa tgacaaatct
     1201 cattatactg cttcgacatg tgaaaagaat aactcatcaa aaaatttaag gaattcttta
     1261 ttgagaataa tatcatcacc gaatatacca aagcttatca ctttaccttt accataatcc
     1321 aattcataag cagctacaag gatattataa atcgattgag cttcataatc aattaaaata
     1381 tttacattgg gattggttat atgttgttct tcgtgttgaa catatgaaaa tggattgtta
     1441 ttaaagtcaa tataacatcc ttgaccgcaa ttgaagaaat tactaccgac ccattctgca
     1501 gtttctgttt tccatctttc caaaaccgat gcccacgcct tctcgccatc aaacgcccaa
     1561 gtgtgtcctt gaattagagt aatctcctca tcgattggat tgtaagcaac ttcagtatag
     1621 aaaacatttg aatcaaataa aataagaata cctccatttt ctacatattc tttcaaatta
     1681 taatattctt cttcggttac atattcctga tgccctaaaa ttataaagtc ataaagattc
     1741 tgattattat tatcaaaaac taatccatta tgtatatccg tgtctgttaa aaatgatatt
     1801 tccatatttg gtgataaaga tgaaattcgt tttgcaagat attgtaaacc atcagatttt
     1861 tgattaagaa aagcttcggt catgttagga acatttgctg ttaataaatc aagatcggtt
     1921 gttacaaatt cattttcact aattcctgca tatcttttat aaaatgtgta aaatgaatca
     1981 ctcttgtagt tgtcgtaagc agccattgtg aatgtaggca ttacaaaccc tattttttta
     2041 ccaggtttca attgaaaagg agtagtagta tttgctattt catcttcttt atcttcaaat
     2101 aattgatttg ttatttgcct atttttaata atttccttta aataataatt ttgaatagtc
     2161 atctctgtta aatctttttg gttatattgc tttgtataga ggtcttcata actatcagtg
     2221 ttatcttgtc cttgggagaa tatctcaatt gatggttgaa ctaccaaaaa aatcattatt
     2281 attgagatta atgatagcga aattgtaact tgcactatca acttgttata attttgttta
     2341 aaaagtaaat gtaagaatat atctatacat tagtttactt aattcttatg ttacttaatt
     2401 tcttagttat tttaataaga atctttagta tctcccaaga tactctactt ggaagaagat
     2461 atgttgcttt agtgttttaa aatcaagaaa atcactatcc aaatggatca tcacatgtat
     2521 catcttcata atctgcttta tctgctcagt tttattagta actaaatggg ctcaataact
     2581 aaaatcccaa gattagaggg cttcgaattc tcattataac agggttcggt aacataatat
     2641 taaaagattt tgttattaaa attgaaggtt aagactcaat taagtgtcaa ttattatgcc
     2701 atggataatt gcataattcc ttttttatat taacaatata gcaatactta taatcaaact
     2761 atagtcaccc taattaacgt gtcaacaaca acaaagttgc agaaaaaaag cctaaattct
     2821 cctgatgaag ttcgaacatt tgagaaaggt aaaatagaaa tcggaaacat tggtgatgcg
     2881 agtattggaa gaagcacttt tgaaccagga tggagttggg agagttgtgt caaacccata
     2941 gtcaaaacaa acagttgtga ggctccacat acaggataca tagtatcagg aagaataaag
     3001 gttgtaatgg atgatggaac tgaaggtgaa gctggacctg gagatatagc tgtaattcca
     3061 cctggacata atgcatgggt agttggaaat gaaccatgtg ttatgattga ttttacaggt
     3121 gcaaaggact acgcaaagaa tagttagatg atataaa
//
LOCUS       DASVEV010000066        14716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_213210, whole genome shotgun sequence.
ACCESSION   DASVEV010000066 DASVEV010000000
VERSION     DASVEV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213210"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1398)
                     /locus_tag="VF222_03305"
     CDS             complement(535..1398)
                     /locus_tag="VF222_03305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7141486.1"
                     /translation="MNSNRNKKVAVVTGSSSGIGLETSLLLARNGFYTYVTMRNLDKS
                     KTITDLKQKEKLSLEVLKLDVTADESVKEAIHRIINEQGRIDVLVNNAGYALVGPFEE
                     LSIEEFKEQFETNVFGVIRVTQSVLPIMRRQKWGTIVNISSIAGKIGFPLTSAYVSSK
                     FALEGLCESMACEVEQFGIKVILIEPGVIKTNFDNNLKIGKRVSIVPNSSYAEMTQKR
                     ISGFKPRFESGSPPIEVATVILKAITSENLSELRYLVGNDAFKLMEIRDNISDKEFRK
                     LIMKSVLQFQQ"
     gene            complement(1610..1885)
                     /locus_tag="VF222_03310"
     CDS             complement(1610..1885)
                     /locus_tag="VF222_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141487.1"
                     /translation="MSQKSLICIYTNIDRNEFAIGLKEDENFRAMTKGGEFYTRFVDV
                     FDQIVDENEIDWKQELKKWINAAKKNETIFVEVDLSGCKGVVQRKIN"
     gene            complement(2304..2474)
                     /locus_tag="VF222_03315"
     CDS             complement(2304..2474)
                     /locus_tag="VF222_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141488.1"
                     /translation="MSSEIHKNVCEICGQKAIAGILDNYDSNNQIRIRYSCHDHYLQV
                     YKKIKVERNNKK"
     gene            complement(2482..2670)
                     /locus_tag="VF222_03320"
     CDS             complement(2482..2670)
                     /locus_tag="VF222_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX7141489.1"
                     /translation="MIQIISLQEITRLTKANTRSNSLRATIPNNVVKELGLQESDFIE
                     WDVFEDGNEKYAKIKKVK"
     gene            complement(4468..5205)
                     /locus_tag="VF222_03325"
     CDS             complement(4468..5205)
                     /locus_tag="VF222_03325"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7141490.1"
                     /translation="MHTEPIIEIRKLKEINIEGGVVFDGFHGIGLTSTIAIGCFINSL
                     KTELVGILDSPLFPPISVIYDTKPNFPARIYANEEKKLAFFVSEAILESSAYRQIAHT
                     ILNWSTHNKCKTVISVAGREIEKEDRTKKEHSLYVISNSPIIMKELNDVGILSLKNGT
                     VNGIPGILLNEANWKNIDVVVFVVDVISGVPDFRTAANVTQVVSKIVPEAYCEIKPLI
                     KEAERIENNLKMVRSQTSNKFKERMYR"
     gene            6117..7925
                     /gene="polX"
                     /locus_tag="VF222_03330"
     CDS             6117..7925
                     /gene="polX"
                     /locus_tag="VF222_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX7141491.1"
                     /translation="MSTNSEVAKIFREIAFILQTAEEKKSEPTTIFKIRSYNRAADEI
                     ENLYSDIGEIYKKEKLKGLLEIPSIGKAIATKLEEYITTGKIHYYDELKEKLPVDVSQ
                     FFGLEGIGPKTIKTLYGNLGIKNISDLEKAALEGKIRSVPGFSQKKEELILKKIQFFR
                     KGGGRRLIGDVYPLVRRIEERLSGNTGVKQAVAVGSFRRMKETIGDIDYLVSVMSEKD
                     GDNIIDYFIDMPEVKEVSGRGSSKAFVKLNNGIDADLLVVPEESFGAAMQYFTGSKEH
                     GVAMRKIAISKGLHLNEWGVFDKQSHRIAGVTEEEVYQVLGLEWIPPEMRENRGEIKL
                     AKMGKNGMEKEEEKVGLPQLIAYDDLKGDLQVHSNNTDGTVSIEEMALNARDKFGLSY
                     IAISDHTKSLRLTNGLDEKQLLNQANTIAELNDKIKMNNNNKNKHFRILSSAEVNILK
                     DGSLDISNNVLDKLDIVGAAIHSHFSLPIELQTARLIDAARNPSIDIIFHPTGRIINR
                     REGYPVNIPKLIEVANDSNTILEIDAHYDRLDLKDEFIRMAVENNVKLVIDSDAHHPL
                     HYAFLIFGIGQARRGWAKKSDILNTLSAKGLLDSLK"
     gene            8333..8740
                     /locus_tag="VF222_03335"
     CDS             8333..8740
                     /locus_tag="VF222_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141492.1"
                     /translation="MKRNNNFFRIRWLDFRQGHSIYLAFLIYFADSILIQYALLIERF
                     PFIDWLVGGNMVGFAITFIALYVPLAIIIGCWHRKSQWKIEVEALFKENELGATMWLF
                     VIDLVDGKITEKEMQEMREMLLRITNGKGKSNT"
     gene            complement(9353..9673)
                     /locus_tag="VF222_03340"
     CDS             complement(9353..9673)
                     /locus_tag="VF222_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141493.1"
                     /translation="MIITTKTKNIVLLALVSTFIMSIVSYTTQQQSFAQGLFQSQQSE
                     RNQSSVSPPSTTEEKEDKSEVAKPATNDDQKIILGNITIPINSNTQLSLEMPDSKITV
                     EPNE"
     gene            11251..11835
                     /locus_tag="VF222_03345"
     CDS             11251..11835
                     /locus_tag="VF222_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141494.1"
                     /translation="MVQIETKSLILTAATIVTILTLTLPSLDLNGFTQTPQILDVQSN
                     SNIDIREIKDNTQSELAVKVQLVPHENEFLEDYYQVSDFEFVLSNSSQLCPTGTCEYE
                     LEDGTMQAERVPGERSLSGRITIDTGESKKMMELRASWNTIEELDKNGKNVKVIKGVL
                     DLGISQFSPENKYQINGTLTTDSDGYLLEVKGIK"
     gene            12845..13258
                     /locus_tag="VF222_03350"
     CDS             12845..13258
                     /locus_tag="VF222_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141495.1"
                     /translation="MKEINGNDIITMAKKILLIDDEPDVTYTIKKILEDNGFKVDSFN
                     DPILALNYYKVNFYDLLILDIKMPKMDGFELYTKIREKEPKVKICFLTAIATFNEEFR
                     KIRLTLGKTINEDYFIQKPINMEDLTKKLTSIMNI"
     gene            13779..14087
                     /locus_tag="VF222_03355"
     CDS             13779..14087
                     /locus_tag="VF222_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141496.1"
                     /translation="MNREEILRSLFTKATEDTIETLPYTIQVFQDPLYDLKRTVTNDL
                     DFLLGAFFAQVLLFYSVYCSNKDIKPTVEESSRLNYELFSKAEEYKNMIGELMVIDNT
                     "
     gene            14490..>14716
                     /locus_tag="VF222_03360"
     CDS             14490..>14716
                     /locus_tag="VF222_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141497.1"
                     /translation="MSTKRHDRKEEESNETHSTTTTTTEQQFQREQQQAVTKALDETR
                     DNIKKTTNEARKEIPRYTQIVNDYQENTIQA"
BASE COUNT         5432 a         2052 c         2112 g         5120 t
ORIGIN      
        1 aagatatggg aattgactcc tggaaagtca tttaaagtag tttcatgtga agatcctttt
       61 ggtaatataa ttgatatata tactcatagt tatgagttta tttggagatc agaagaatat
      121 caattatgaa ataaattaga gtgttattca taatttccaa attagattat gacaaaactt
      181 attaaaatta agttcttcta atgcctaatt caacatagta caagttcttt attaccatac
      241 attagactct tattttagca ccgctatcgg tattactctt gacataataa actcatgata
      301 atacttctat aaagatatct caaattatat ccgtaaaggc aaatgggata aggatatatt
      361 tgaatttaaa atacagatgt aatatttgaa ataatattta gaagtaaaaa attagaaaat
      421 atatatattc acattaagtt aaataatata attagcccca aatgaataat attataaatg
      481 gtctaatctc aaaaatacgc tattattttt tggaatatta agtattggat attgttactg
      541 ttgaaactgt agaacgcttt tcattattaa ttttctaaat tctttatcgg atatattgtc
      601 tcttatttcc atcaacttaa aagcatcatt acctaccagg tatcttaatt ctgatagatt
      661 ttctgatgta attgctttta aaatgaccgt tgctacttct attggaggag aaccactttc
      721 aaatctaggc ttaaaccctg aaatcctctt ttgtgtcatt tctgcatatg acgaattagg
      781 aacaatactg actctttttc ctatctttaa attattatca aaattagttt taattacacc
      841 aggttctata agtataacct ttatcccaaa ttgctctacc tcacaagcca tagattcaca
      901 taatccttct aaagcaaact ttgaacttac ataagcagaa gtaagaggaa atcctatttt
      961 accagctatt gagcttatat ttactattgt accccatttt tgtcttctca ttattggtaa
     1021 tactgattgt gtaactctta taacaccaaa tacattagtt tcaaattgtt ctttgaattc
     1081 ttcaatagaa agctcttcaa atggtcctac taaggcatat cctgcattat taactaaaac
     1141 atctattctt ccttgttcat ttattatcct atgaattgct tctttaacag attcatcagc
     1201 agtgacatca agctttaaaa cttccaaaga caatttttct ttttgtttta aatcagtaat
     1261 tgttttagat ttatcaaggt tacgcattgt tacatatgta taaaaaccat ttctggccaa
     1321 caacaaagag gtttcaagtc ctataccaga agaactacca gtaactactg caaccttctt
     1381 atttctgttg ctattcatta tactgttatt atcattaagc tagataacgt gttaatactt
     1441 ttgttcatta tataattgta gcatgcacga tactgcttag caactctatc tgcatttagc
     1501 tgttttccaa tttcttttgt tctctctgct tcttcgattc aaaatctgaa gaattaggat
     1561 tatgtttgta ataggtaaaa atatctagag ttcatcttcc aataaatatc taatttattt
     1621 ttctttgtac tactcccttg caaccactta ggtcaacttc aacaaatatg gtttcatttt
     1681 tctttgcagc attaatccat ttctttaatt cttgtttcca atctatttcg ttctcatcta
     1741 ctatctgatc aaaaacatca acaaatcttg tataaaattc acctccttta gtcattgctc
     1801 taaaattctc atcttccttt aacccaatag caaattcatt tctgtctatg tttgtatata
     1861 tgcaaattaa tgatttttga ctcatatcgt tattattatt actattatta ttgaggttgt
     1921 tattattttt taatttttat ctcatctgta aattacagta tatgtattaa aaattattta
     1981 tttttattca ttatttatga tactggttgt tatttgcata attcaatgcc taaacacttt
     2041 aaacaccaaa attcgttggg tcctattcca tcacctctat cttttcttac tacaataaaa
     2101 attgatggat gttctatacc gaaattctca catttccatt ataactcatt gccattttct
     2161 ttgttattta ttttccagta tttatttaaa ttaattttgg gtataaacat ataatatgga
     2221 aacaaagttc tagaagaatc agattccaca tggaatccga atgttataaa gaatcttcct
     2281 caactggaat aactctatag aactcatttc ttattatttc tttctacttt gatcttttta
     2341 taaacttgta aatagtgatc atgacatgag tatcttattc tgatttgatt attactatca
     2401 taattgtcta aaatacccgc aattgctttt tgtccacaga tttcacaaac atttttgtga
     2461 atttcagaac tcatttttca attactttac ttttttgatt ttggcatatt tctcattacc
     2521 atcttcaaaa acatcccatt ctataaaatc cgattcttgc aatcctaatt ctttaactac
     2581 attatttgga atagtggcac gtaaagagtt agatctagta ttagccttcg ttaacctagt
     2641 aatctcttgc aatgaaatta tttgtatcaa ttacctatat atcctacacc atagtgaaaa
     2701 taaaagttac ctaattaaaa ttattaccta atatttaaaa ggaaagctaa tgtctataat
     2761 gtagggagct ttgacaacct aattagaaaa tcaactcgtt attgagaaaa aaatagaagg
     2821 tcaggaaagg gttttgagat tacacaggaa cgtattgatg taattaatac aacaacagaa
     2881 tatttacaca ggttaaatga tttgaaaagt atgcaatgat aattttatgg taccaatgtt
     2941 ggacaatgct attatatctt tgaagatttt gtaaagtact aagcatctaa tgccaaaaca
     3001 agtaaattaa tacatcctcc ttaaataaat aataaatgaa taaatagatt gcttgatagt
     3061 tgattaaatt ctcaaattag gtttacaaga attaataaat acaataataa ctatgataat
     3121 actaccacca aatatctcac atatctcaac caatcaatca ctttatttgt catagtcgga
     3181 acaatcccag ccagatttca tctattcgac tatgacaatt tattaactat atgataaata
     3241 aatttcttat taaattatta aatattatat tgggacatat attatattac aatctgtcaa
     3301 cacatatcca tagcagggcc atagtcttgc tattctgtta tacctctgtg ctccatttgt
     3361 agtaggagta gtatggctat ggttcttcta ttattgttag tcgattaact tggtttagta
     3421 attttcaata tcttgagtat gatgtgatta tattacaatt cactacattg ggggaaccta
     3481 tcatgagcca tagtctgtgt gtttatgtat atgtgtatac tctgtcgtgg tcttaggcat
     3541 acatatacag gcatatggtt tttagtgtga tagttttaat taattaatta atttattact
     3601 cgagatagaa agataggtag agaatattat tatgctcttt tactataatt tttacaaata
     3661 gacattttca caaatgataa atagtaattt tattaggtat ttctgatcta attcactacc
     3721 aagatcaaat aggaaaacca aaaatcgtct taatcttgtc atagtctgac cggaataaca
     3781 acattgcttg catcagcagt atgttcaggc tatgacaata ataaattatc tcaaatgact
     3841 aaataagatg tatcgtaaag attgttacag ttcaaagaaa cgatagattt gattttcaaa
     3901 gccttctatt attggattta tccctcaata gtagtggatt atggctttta ttattctctt
     3961 tagtattcta tctatatcaa tccttgatgt aacatacttt tctcttatgt attgcatata
     4021 tttaattcaa aattaagtag caacattcac taattcattc attacctgtt ttcgaaataa
     4081 catcgattga taatttccaa caaaaataaa aaaagtaata taaaaaatgg gcaagacaat
     4141 aaagaaaatt aagataataa aaaagacttt gataaggaaa ataatatttt tgttagcaat
     4201 aatattataa ctactgtaaa atacaaaaaa ataatgaatc tacaaataat gaaaacaagc
     4261 cagagaaaaa aactgatgag cgaatggaaa aaaagattca tacaaagttt tctatttatt
     4321 tattatatca aagtttctca ggctctgtta agttaaggat aatttatgaa ttaagaaaat
     4381 agactaaaat tggcacaaaa cataatagaa aaatgtacat actactactc tatccataaa
     4441 gttaactgag cctgtaaaat gataatttta tctatacatt cgttctttga atttattgga
     4501 tgtttgactc cttaccattt ttaaattatt ttctatcctc tcagcttctt taatcaatgg
     4561 ttttatttca caatatgctt cagggacaat tttgcttaca acctgagtaa catttgctgc
     4621 tgttctaaaa tctggtactc ctgatatgac atcaactaca aatacgacga catcaatatt
     4681 cttccaattt gcttcattta aaagaattcc aggtattcca ttaaccgtgc cattttttaa
     4741 agataaaatt cctacatcat ttaattcctt cattataatt ggagaattcg aaattacata
     4801 gagggaatgc tctttttttg ttctatcttc tttttcaatt tccctacctg caacacttat
     4861 tactgtcttg cacttattat gagtggacca attcaatatt gtgtgagcaa tttgtctata
     4921 ggctgatgat tctagaattg cttcagatac aaagaatgct aactttttct cttcatttgc
     4981 gtagattcta gctggaaaat ttggtttagt atcataaatt actgatatag gaggaaaaag
     5041 aggagaatcg agaataccca ctaattcagt ttttaatgaa ttaataaaac atccaatagc
     5101 tatagtacta gtaagaccaa tgccgtggaa accatcaaat acaacaccgc cttcaatatt
     5161 aatttccttt aattttcgaa tttcaataat tggttcggta tgcatgaata tgtttataat
     5221 ttcattattt taaactgttt gcttaatggt tctaaacgca taggtaggtt catcttgtat
     5281 aaacattaga gaattgaaaa attacctgat ttgaatttct gattttcatc taatctaaat
     5341 attgaatctt taatttagat attatttatt ttattttagt gaatattaat aatataaact
     5401 agaaatattg ctttgaagtt cgaatgtcaa ctcaagaaga acttatttta ttttaacact
     5461 tttactttat agataaaaaa taaattttaa tattttatat attatatttt aaggtattcc
     5521 attaattaat atgtgaccta tatacctata taaaggaata tttattaaat ttaaataatt
     5581 gtcattattt tttttaatta gattcttatt cactatgata aagattcaaa agaagtaaat
     5641 atcctaaaga attttgataa ttaaacaaaa gtttgaagaa ataaaagata taaaacaagt
     5701 actcaataac tcaaatggta agctagatat tcattaacag aattttccta tcaaatattg
     5761 tacaaaaccc atttaattaa tatttacaag ataaaaaaat agtaacatgg aaactacaaa
     5821 tattaaaaaa tttgaaaaca gacctaagaa tgctatcaga ctattggctt tgttagaagc
     5881 aaaaaaggga aaagatcaca cgtaaaaaac aaaaaccaaa ttatttcatt gtggatacag
     5941 aaataaaaaa taaggtagat gacccagatg aatactggat aataacctgg aatgattacc
     6001 cggtaagaat aaaatacttt ttcagatggt tatttatcgt accatttaat tgctctttca
     6061 atgtctcgtt aaatcgtttg gtttaagtct ggtcgtgcta caaagaaaat gtagcaatga
     6121 gcacgaattc tgaagttgcc aaaatttttc gagaaatagc atttattttg cagacagcgg
     6181 aagagaagaa aagcgaacct actacaatct ttaaaattag atcatataat agggctgcag
     6241 atgaaataga aaatttatat tccgatatag gcgagatata taaaaaagaa aaactaaaag
     6301 gattgctaga aataccctca attggaaagg ctattgctac aaaacttgaa gagtatatca
     6361 caacaggtaa gatacattat tatgatgaac taaaggagaa gttgccagta gatgtcagcc
     6421 aatttttcgg tttggaaggt attggcccaa aaacaataaa gacgctgtat ggtaatctag
     6481 ggatcaaaaa tatctctgac ttggaaaagg ctgcgttaga aggcaaaatc agaagtgttc
     6541 ctggattttc tcaaaagaag gaagaattaa ttctaaagaa gattcagttc tttagaaaag
     6601 gtggaggacg acgactaatt ggtgatgtct atcctttggt aaggcgaata gaggaacggc
     6661 tttcaggaaa tactggagta aaacaagcag tagctgtcgg ttcatttaga cgaatgaagg
     6721 aaactatagg agatattgat tatttagtgt cagttatgtc tgaaaaagat ggggataata
     6781 taattgatta ttttatagat atgccagaag ttaaggaagt aagcggaaga ggatcatcta
     6841 aagcatttgt caaactaaac aatgggattg atgcagattt acttgttgta cctgaagaga
     6901 gttttggtgc agcaatgcaa tactttaccg ggagtaaaga acatggagta gctatgagaa
     6961 aaatagccat atctaaaggt cttcatctaa atgaatgggg agtttttgac aaacagagcc
     7021 atagaatagc tggcgtgact gaagaagaag tgtatcaagt attgggccta gaatggattc
     7081 ctccagagat gcgagaaaat agaggagaaa taaagctagc aaaaatggga aaaaatggaa
     7141 tggaaaaaga agaagagaaa gtagggctcc cacaattgat agcatatgat gatttgaaag
     7201 gagacttaca agtacatagc aataatacgg atggcacagt gtctatagaa gaaatggcat
     7261 taaatgcaag agacaaattc ggattgagtt acattgcaat ctccgatcat acaaaaagtt
     7321 tgaggcttac taatggacta gatgaaaaac aattgttaaa tcaagcaaac acaattgcag
     7381 agctaaatga caaaattaag atgaataata ataacaagaa caagcatttt cgtatattat
     7441 cttctgcaga agtaaatatt ctaaaggatg gatcactgga tatttcaaat aacgtcttgg
     7501 acaaactaga catagtaggt gctgctattc attctcattt ttctttacca atagagttac
     7561 agacagctag actcatagat gcagctcgaa atcctagtat tgatattata tttcatccga
     7621 cgggcagaat tattaatcga agagaaggat atcctgtcaa catacccaaa ctgattgaag
     7681 tagcaaatga ctccaataca atccttgaaa ttgacgctca ttatgaccgg ttagatttaa
     7741 aagatgaatt cataagaatg gcggtcgaaa ataatgttaa attggtaatt gattctgatg
     7801 ctcatcatcc tttacattat gcttttctaa tatttggtat aggacaagcc agaagaggat
     7861 gggctaagaa gtccgatata cttaatacac tgtcagctaa aggattactt gatagcctca
     7921 agtagtagac tagagcattc attcttaccg atgaacaaaa tagaaataga tcctatatcc
     7981 ttaacgttca tacgtacaat atcgtcgtct attattgact tttataatgc gagtggtagt
     8041 acatagatgt cctctatatt cgattttgga ttatttcatc aatagttctg tatttttata
     8101 tgagaaaagt aaccaaaaag agtttaaccg agataaacca aatgtgtttc tatcattact
     8161 aaatattttt gaaaggctat acatgtggtt tctttatata aatttggaaa tgaaaagaag
     8221 tagctgatat gcaaaaattc ttggttcaat gtaaaaactt ttgtctcaaa ttattaaata
     8281 ataagatgga aatacaatag agaacaagct gccaagaaaa aatcctaata tattgaaaag
     8341 aaataataat ttttttcgaa tacgatggtt ggattttaga caaggacata gtatatacct
     8401 tgctttttta atatattttg cagattctat acttattcaa tatgcattat tgattgaacg
     8461 atttcctttc atagattggc tagtgggagg aaatatggta ggatttgcaa taacattcat
     8521 agcattatat gtacctcttg caatcataat aggatgctgg catagaaaga gccaatggaa
     8581 aatagaagtc gaagcattat ttaaagagaa tgaacttgga gcaacgatgt ggctttttgt
     8641 catagatctt gtagatggaa aaattactga aaaagagatg caggaaatga gagaaatgct
     8701 tttaagaatt acaaatggaa aaggaaaatc caacacgtaa gaatctacaa ataatgaaaa
     8761 caagcaagag aaaaaaactg atgagcgaat tgaaaaaaag attcatacaa aaaatcacat
     8821 cacaaaggaa gaaaaattaa gtaaaagtat gcaaggtcat tcaccaaaat atgaatagta
     8881 atttagagaa taatatgtta cttaatattt tcaacaatgt tacttcctaa cttccagtta
     8941 ttgatgttat taaatttatc ctgtataagt atctaatgtt atttctgttt tgaaattttg
     9001 ttttaccaac gtttgtatta tattcaactc tctatctatg cgcttctgat ctgatgtaat
     9061 ttaaaaaaaa gaataagtag ataaaatcta aaatacaatt tactatatgt caaacaattc
     9121 tattactact actgcatctg atgataataa ttataatcct gaagatatta tcaaaaagga
     9181 ttatttcaga gcaaaattaa actatctaaa aaaatttatc tgataaaacg agactcaaga
     9241 tcatttattg ttttgttcac cataatagat gataaattaa agactatgat gaagactaat
     9301 aatgtttttc taagatataa aatgtcaata aaaaagaata agatagtgga agttactcgt
     9361 ttggttctac tgttattttt gaatctggca tttctaaaga gagttgtgtg ttagaattta
     9421 ttggaatagt aatattacct agaataattt tttgatcatc atttgttgct ggttttgcta
     9481 cttctgattt atcctctttt tcttctgtgg tgcttggtgg agatactgat gattggttac
     9541 gttctgattg ttgtgattga aacagacctt gtgcaaagct ttgttgttgt gtggtgtagg
     9601 atactatact catgataaat gtcgagacta aagccaataa gactatattt tttgtttttg
     9661 ttgttattat catgttataa actcgaggag agaagtattt aagtttttat cattttcccc
     9721 ttcaaaataa gttagaattt atggaatgtt ttattgatct tcatttaata ttacaattca
     9781 agtattttac aaattgtttt ttatgttatc ttttggaaaa gaatggattc gtagaatagc
     9841 atttaatcca gcctaaatta caataacata attaaataac atgtattgat aatgcaatga
     9901 acctatggtg aatcgatttg aaatcacata tactatatta taaaagaaga tgatgatgat
     9961 gatgatattg ctgaggatct aggatatacc aatacaaaat atacatgatt taatattcta
    10021 taaagttcta gggtattatt gtaaataata ttgtaaccgt ttatgtaatt ttattcaaag
    10081 tagtatgaat tgaatattga gatatatttc ttcaactccc gaaggatcag tattttccta
    10141 gggttcatga ctccacttaa atgattctaa attttaataa tggccctctt aaattatctt
    10201 gaatattgat atcttttatt actagatgca tatatagtaa ttaaattata aataatagac
    10261 tatgattgaa tgcagacatt gggcatgtaa tctaagaaat aatacttgta ttggttgtgg
    10321 taatgctata tgtccaaatt gtttattaga gccatctgat tgtagatgtg ataataaaga
    10381 taattgctgt aaagtatgag attaaaaata tctcaaatag attgcttaca aatctttaat
    10441 ttaaccatta aaagtctcaa aatatttaca aataacttcc tgataataaa agctaaatac
    10501 aagttattag aatccctaat atatacagga ctttattgca gatgtttgtg tctactaata
    10561 agttgattaa catataaaaa attttaccta ataaccaata caatctcttg acagaaataa
    10621 atttgattat ccataattga ataaatctga caagttttgt actagaaaag acaagaataa
    10681 tagagttcga aatttgatta tagtataaat ttgttacttc tagatatatt ttaagtaatg
    10741 tgcaatgaat gtaaatgagg gacacaaagc caagctcatc ataggataga aacgttgaac
    10801 ctaaatctgg gaatggccat ctcttctctc agcctggaaa atattctcta ataacatttt
    10861 gtatgaatat tttcaaagag taaattttaa ttggaacaat aaaaagaaca taatataatt
    10921 aaaagtttca gtatttttgt atcaatttta caatcccaaa acactgaatt ccgcccacaa
    10981 gatggaatct tggagtagat tttaatcttc ggcccagatg ccgctaacca agtagtccca
    11041 gttcgaataa caattgccta cgcttacata atgtattcca gatgcactta acagttactt
    11101 ccacgaacat tatgaacact aatgggcttt acttatgctt gaatttttgt tctttattaa
    11161 cataatattt ttatcgctag ccttcatcca agatttatcc attctatatg gtattgcaaa
    11221 ttttatatag gaatatttgc tgttattctt atggtacaaa ttgaaactaa atcacttatt
    11281 ttaactgcag caactatcgt gacaattcta actttgacat taccaagtct agatttaaat
    11341 ggatttactc aaactccaca gatactagac gttcaatcta attctaacat agatataagg
    11401 gaaataaagg ataacactca atccgaactt gccgtaaaag ttcagctagt cccacatgaa
    11461 aacgaatttc ttgaggatta ttatcaagtt tctgactttg aatttgtgct tagtaattca
    11521 agtcaattat gtcctacagg tacttgtgag tatgagcttg aagatggaac gatgcaagcc
    11581 gaaagggttc caggtgaaag atcgttatca ggaagaataa ccattgacac aggagaatcc
    11641 aaaaagatga tggaattacg tgcttcttgg aatacaatag aagaacttga taaaaatggt
    11701 aaaaatgtta aagtcataaa aggggtatta gacttaggaa taagtcaatt cagccccgaa
    11761 aacaaatacc agattaatgg tacactaaca acagacagtg atggttatct gttagaagta
    11821 aaaggaataa aataattaat cttatattat ttttagattc ataatcatag tatcacagtt
    11881 aagctattac atgttatcat ctctcataat ttcatccaga atattctgat actcactact
    11941 gttttttcaa caagtttaca tttattatta taactaaata aataggttac aaatctatgt
    12001 ctcaccttaa cattgtcaag gtctaccaca agtatttgaa acatgtgaac aattgaagaa
    12061 atagtagcac gaaagttagt agtataaaat tattattatt agactttaac aaaaatttta
    12121 acaaacccat gttcaaaatt agtttatcta aatatgtaca cacgttttac acgcaaatat
    12181 ataaaagcta aatatacgtt aaatgaacct tatttcatgg agtattttta ctctggccga
    12241 agttgctaaa tatatagatg aattctattc ttaaaatggg tggcccaaaa acagtatttt
    12301 gcaattttat gtttatagtc agaaatagta agaatactga ttttttaatt gcttgacctt
    12361 ttatactcta tatacaacac cctttcactc attctaacat ctgatattca agatgcagaa
    12421 tggattatgg ctttatcatt atgatgtaga atttatgtat aacaattaat ttattgtgcg
    12481 tttataaagc taattattat ttaatattat gagtaaatcc actacaataa ctattacaac
    12541 aaaaacgaat acacattttt agcggctgta ttagttgcag aataattgct gtatcatctc
    12601 cgtcttttat tgttgtagta ggtacagatg ataaatgtag ataattataa gtatttactt
    12661 ttttttattt tgttagattt attctatatc ctctggtaag tattttatca catctatatt
    12721 attaataaaa tagtattttg tgatatgtat acataatcaa aaataaagta aaaggtaaaa
    12781 aatttagaac gttaagaact catccaatct ttaaacacta ttatactcca atggttaaga
    12841 tataatgaag gaaattaatg ggaatgatat tataacaatg gctaaaaaaa ttttattgat
    12901 agatgatgaa ccagatgtca cttatacaat aaagaaaatt ttagaagaca atggatttaa
    12961 agtggatagt tttaatgacc ctattttagc attgaattat tacaaagtca atttttatga
    13021 tttgttaatt cttgatataa agatgccaaa aatggatgga tttgaattat atactaaaat
    13081 aagagaaaaa gagcctaaag taaaaatatg ttttttaact gcaattgcaa cattcaatga
    13141 agaatttaga aaaattcgat tgacattggg taaaacaatt aacgaagatt actttattca
    13201 aaagcccatc aacatggaag acctaactaa aaaattaact tctataatga atatctaaaa
    13261 caatcataga tagatgtcat ctatgttatt gatatgttaa agtaaatctt taatattatc
    13321 tccttaatta ctatatatta gactcttatt ttagcaccgt tatccgtata accaccggtt
    13381 gtttagaaga ataagatatc agaaattttt tcaaatataa tatttttcaa tataatgtaa
    13441 ttaaaaaaga ttagcaaatg gattctactt tcataatcta gttgtaagtc tatagcaact
    13501 tcaatagaaa tttccgaatt gtttttcttc taaatataat tctataatcg tttgtctttt
    13561 tcaaatttgt ttaaaaagaa tttcagatat tcttataata gctcttaaaa aaagaaatat
    13621 ttaaatggta acattgtttt tgtgtgttat caattaatta ccattgttac catttacttt
    13681 attatggaaa ataccatgaa agatttaact aaattagtta gtgtgataca tactccataa
    13741 aataaaatct tttaacaagt tcatattatt aataaatatt gaatagagaa gaaattctta
    13801 gaagtttatt tactaaagct actgaagata caatagaaac tctgccttat acaattcaag
    13861 tatttcagga ccccttatac gatttaaaga gaacagtaac caacgatttg gattttcttt
    13921 taggagcatt ctttgcacaa gtacttttgt tttattctgt ttattgttcc aacaaagata
    13981 ttaaaccaac agtcgaagag tcatctcgtt taaattatga attattttca aaagcagaag
    14041 aatacaaaaa tatgattggg gaattaatgg taattgataa tacttaatta actaatttcc
    14101 aaatgaaact aaaataataa taagatgctt gatttcatca cgtagtttat ctgagataat
    14161 aatctaatat gtaatactaa aacacatcat aatatcatgg aaatagtatt ataaatatga
    14221 aagattagaa ctgttaaaat gctaaaagga ttaactgaga tttaccgaaa gtttttttac
    14281 cgttgaaaaa atattagatt gtggttcgtc tcgtcttgcc gaaatattaa gaatagatac
    14341 ttatgttcga aaattaatat aaaaaaccta acagtcaatc atcactgtac actattatta
    14401 ttagtgcatt attagtgcat gttattagta atccacacca ttgtatacta ttagttatat
    14461 gctatcatgt atactaatta tatatatata tgtcaacaaa aagacacgat cgtaaagaag
    14521 aagagtcaaa tgaaacacat tcaacaacaa caacaacaac agagcaacaa tttcaacgag
    14581 aacaacagca agcagttact aaagcattag atgaaactag agataatatc aagaaaacaa
    14641 ctaatgaagc aagaaaagaa attccacgtt atacacaaat cgttaacgat tatcaggaaa
    14701 atacaataca agctat
//
LOCUS       DASVEV010000067         9861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_990343, whole genome shotgun sequence.
ACCESSION   DASVEV010000067 DASVEV010000000
VERSION     DASVEV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990343"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VF222_03365"
     CDS             <1..669
                     /locus_tag="VF222_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7141498.1"
                     /translation="NIAAHELRTPIMPILGEVEIIEEDLDPKTKTVQVEEEQIQLIIR
                     NAKRLDRLASDILDVTKIESNSLKLEKVYFNLNDVLSNSIRDIQNQVSSGDIGTKNIK
                     IVYEPVHIKISGDKERINQVVSNLLSNAIKFTDEGFIKIKIQNTNDSVIISVEDTGSG
                     IHPEIFPRLFCKFATKSDKGTGLGLYISKNIIDAHGGQIWAQNNSSGIGATFFFSLPL
                     GKKK"
     gene            complement(1092..1166)
                     /locus_tag="VF222_03370"
     tRNA            complement(1092..1166)
                     /locus_tag="VF222_03370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Gly,seq:ccc)
     gene            complement(1238..1513)
                     /locus_tag="VF222_03375"
     CDS             complement(1238..1513)
                     /locus_tag="VF222_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141499.1"
                     /translation="MLDEVDAEELPTQTTAISGVQGSSLSAMINNALWGNVNSNLPDS
                     TASEYVMEQFITINPDGYNDNIIFEEPKKKVELLNSNVPFILPVPFP"
     gene            2418..4055
                     /gene="pyrG"
                     /locus_tag="VF222_03380"
     CDS             2418..4055
                     /gene="pyrG"
                     /locus_tag="VF222_03380"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HEX7141500.1"
                     /translation="MQLTRNKLKDHHIKYIFVTGGVLSGLGKGVISSSIAKILQLTGL
                     NVSCVKVDPYVNYDAGTMNPVAHGEVFVTCDGGECDMDIGNYERFLNIELSRDHNITT
                     GRIYSEVIGNERQGKYLGQCVQIIPHVTDAIKNKLRQISIQEDLDVLVIECGGTVGDI
                     ESLPFLEAFRQMKLEEGDYNTLFVHVTLAPILDVVGEQKTKPTQHSVQELRRIGINPD
                     LLAVRCKNTLSEDARRKISLFANISSNCVFSCHDVKSIFLVPEILADQGIVSMISNKL
                     RLQNLFIKWSNWKLIAKSFFEYSKEVHIAIVGKYVSLPDSYVSVYQSLSHAAAKLGNK
                     LEVAWIDSEKFDTNSKEKISLLKKFDGILIPGGFGKRGSEGIINVANFARKENIPFLG
                     ICFGFQLSIVSFARNACGIDNANSTEIDPDTCNPVVEFMPEQKTINTLGGTMRLGSHK
                     ILINSNTLASKIYSKNFIIKRHRHRFEFNKNYLPVLSKNGMVLSGFSDNEKRTEILEI
                     PNHKFYFAVQYHAEFDSRPGKPEQAFEAFINNASLKS"
     gene            complement(4045..5049)
                     /locus_tag="VF222_03385"
     CDS             complement(4045..5049)
                     /locus_tag="VF222_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7141501.1"
                     /translation="MKIALVGAHPQDTLLISKIKKILDNSFIQTIIMTENNFNLANQV
                     DIVFIVGGDRAMLDYFHKVVDESAPVIGIYESESTGFLAQIDVRDLETNIQRLKKNDY
                     RIDEVVRLAVNVDGKEMEPVLNDVAVFPSKSATLMEHILRIDKYDVWHDNSDGVIIST
                     PTGSTAYSMSAGGPMILQNSKVFVAVSVNSVDNTRRPLIFSDNCILEISDILSRYHCE
                     VVLDGGRRLGIKKILTCSKHQYPAKLVRLIGEGSATSLIAKKVKIAEELLNMPPSAKL
                     ILKILEYEGSMSQKDLANKTLLPERTIRLSLGHLVNRGYVKKKTSLRDARHRTYELKI
                     "
     gene            5255..5542
                     /locus_tag="VF222_03390"
     CDS             5255..5542
                     /locus_tag="VF222_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HEX7141502.1"
                     /translation="MPKKRTSRGRTKGGKGSSGTVHCSQCGAMVPRDKAKKITGRITL
                     VEPTLAKELRAQGAYISPATDVKFYCVSCAVHRGIVKVRSEVERRVNTRLR"
     gene            complement(5543..6139)
                     /locus_tag="VF222_03395"
     CDS             complement(5543..6139)
                     /locus_tag="VF222_03395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7141503.1"
                     /translation="MLNKFRKHLQPILDKTGVLFSSFGLSPNTLSLIGFIITIISSII
                     FGLNSLQLDPLVNFSAIGSLLLLTGGFFDVIDGSVAKITKRTSRKGSFLDSTLDKISE
                     AIIFLGIAIGELANPILCIIAVSASLLVSYTRSRAETLGIDLSGVGFGERAERILILA
                     IMGLLPFSHSLEYGVIIVIGIAIITVIQRIYKTLKMIE"
     gene            6255..6890
                     /locus_tag="VF222_03400"
     CDS             6255..6890
                     /locus_tag="VF222_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX7141504.1"
                     /translation="MSDFDRILVFGAHPDDIEIGMGGTITKLVKLGHDVIMVIATLPN
                     FTIADNKDERKLEAISSATILGCNSPEFLDLAQEDIVFGRRMVTLVDSLIKKYDTSTV
                     YTQWIGDSHQDHQILTQSVIAASRDLNNLYMYETTIPGGLTEKAFRPQLYVDITKEME
                     NKTKSLKCFKSQNLRCGPFWIDAIVGRSIYRGYQMNTKYAEAFEIVKTVQF"
     gene            complement(6917..8056)
                     /locus_tag="VF222_03405"
     CDS             complement(6917..8056)
                     /locus_tag="VF222_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141505.1"
                     /translation="MLKFLKSNKGLVYIILGNIVGALLTGGFWLLLASLQSVEEYGKT
                     SYMISMASLASSFALLGLNTAITTFIPKGHDKIHVAANQVVLISSIIAALIVAQRDWL
                     LGFFVVGMSFWMMSIYEFLGRKSYKQYAIANIGARGCQLLLSIALYYIIGIPGIIIGF
                     TISFFLFSHRYFLSIKYFKKDFSEISGKMKFALHTYSFNMSNALLMYFDKIIIPPIFG
                     YAILGYYQLGFQFLMFLGMVPISFFQYLIPEESSGNKKTKLRIIGFGLSIGLAALLYF
                     LSPIIINVFFPHYQNSLDAIRVISIGIIPMMIAYVINSKLLSEGNTKGVVIGAIIYQI
                     LQLTLMILLGRLIEVTGIALAVVIALTGQALFLLFYKKLSKKGEQ"
     gene            8471..>9861
                     /locus_tag="VF222_03410"
     CDS             8471..>9861
                     /locus_tag="VF222_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX7141506.1"
                     /translation="MKNSDRKFQLLSAVLITLFCCILLINSNYVLVESQTSPTFLTLT
                     GSNFLDVENSERLQLKDFTLSTWFRTDKQDYLEPGTIVNKGGMNDDAPGKNMNYGIWI
                     TSEEFLQGGFETKTGTNIFVTSKQKFNDGNWHNVILTNDGLFLKLYVDGVEIGKISTL
                     GAIPDMKGSQSIRLGANTLNLDKFFTGDIDEVILWDRALSLEEIFNFYNYDEIPSNGR
                     ILYLPFSSISPIQSSYKYQPFKTFEQTEKEVIDGEESFRLSNFSLGTWFRIDRIDHTG
                     PGILLNKGGFGGERPGENLNFGIWLNPSETIEGGFETKPGTNVFVSSQGKYNDGLWHY
                     VLLTYDNKILKLYIDGVLADSKITSGAIPDNSGDQDFVIGGNSFLDDKFFKGDLDEIR
                     VWNRALNTEDVSNGFDKNTFNTNGQIFYESFSDLDTQPPSPPPTETPGTTPPPTETPG
                     TTPPPTETPGTTPP"
BASE COUNT         3581 a         1453 c         1507 g         3320 t
ORIGIN      
        1 aatatcgctg cacatgaatt aagaacacca ataatgccta tacttggaga agtagaaata
       61 attgaagagg atcttgatcc caaaactaaa actgtccaag tagaagaaga acagattcaa
      121 ttgataatac gtaatgcaaa aagattagac agattagcat ctgatattct tgatgttaca
      181 aagattgaaa gcaactcatt aaagctagaa aaagtatatt ttaatcttaa tgacgtttta
      241 tctaattcta ttagagacat ccaaaatcaa gtttcttcag gcgatatagg aactaaaaac
      301 ataaaaatag tctatgagcc tgtacacata aaaatctctg gagataaaga aagaatcaat
      361 caagtagtta gtaatttatt aagtaatgca ataaaattta cggatgaagg atttataaag
      421 ataaaaatac aaaacacaaa tgatagcgtt attattagtg tggaagatac tggaagtgga
      481 atacatcctg aaatatttcc acgcttattt tgtaaatttg caactaaatc tgataaagga
      541 accggtctag gtttgtacat ttcaaaaaat attatagatg ctcatggagg tcaaatatgg
      601 gcacaaaata attcatcagg aattggtgca acgttcttct ttagccttcc attaggtaag
      661 aaaaaataaa aatattttta tctatttatt ctcttctaaa atataacttt cgtttattta
      721 ataataagta tggatacata ataaaattaa taaatcatga aagaaatcat aattagatat
      781 tttaaattat atatatatat atttgtactc atttttactt gtacaacatg agactctata
      841 tttccacact ataaagttct aaccaatttc gaagtgaata ggttcaaaat tataaaaaga
      901 catagaacat tttgtaaagg caataaattt gcactttcac aatataatag aagagattac
      961 tttgaattaa tacataaaac tgcttaccaa gaccattgaa aggttaaaaa aattatcgag
     1021 aagtatatcc aacctgctaa tacaagggaa atattaattt tttaaacttt gaaatgattt
     1081 tttagcaaat atgcgaagtg cgggatttga acccgcgacc ccgagcttgg gaagctcgta
     1141 ccctaaccag gctgagctaa cttcgctaac atccatacta ttctacaaga ccatcaatat
     1201 atttttttta aacttgttaa gaaaggatct gaaatagtta aggaaaaggc acaggtaaaa
     1261 taaaaggaac attggagttt aacaattcca cttttttctt gggttcttca aaaattatat
     1321 tatcattata tccatctgga ttaatcgtaa taaactgttc cattacatac tctgaggcag
     1381 ttgaatcagg taaattagaa ttaacattac cccacaacgc attatttatc attgcagaga
     1441 ggctagatcc ttgaactcca gatatagccg tcgtttgtgt tggcaattcc tctgcatcga
     1501 cctcgtctag cattaaccct atcaataata gcgaaatagc gaatacaaca atagatagtg
     1561 aagttgccgt ctttaatcac ataagtgtat tcaattaagt atatttaatt tgggacatta
     1621 gtatctgtac tattatatga tgataataag caggaaaata aaaactgata ttatgatagg
     1681 tactattttc gaaaaatagt gatatactaa taaaataaaa tacttttatg gaagacttta
     1741 ttgatacaaa aaataatgta attgatatag aaaacaatac tataacatag gatcctcgca
     1801 tgtattaaat ttgtatatat ctttctatat ttatactatt tatacttcta ttatcttaat
     1861 ttcttaaata aatgtgtata aattattcag agttaagatt tctattaaat ataaaaatat
     1921 aaagtaaaaa atgtaacatt tatttggata tacaatatta tgtaggttta ttaataaatt
     1981 aatggtagaa taatgttcta aatactattt atagatattc ataaatatga tacttaaaat
     2041 tattttataa tgctggaata aaaatctaaa ttatctaaat ttatactcta tttatcatat
     2101 ttgtgcagga ttttatgaca ataaaatact aaaacaaaaa tacaataaaa gtaaaatctt
     2161 taaattttta tcttgaaatg aaggtaataa ttagtactat agcaaatata tttctaaagt
     2221 atttatagta ctcttaatac tataaaagta gtatttattt atgtgtctca tttatattat
     2281 gatatttttg aatattaatt attattaatg atgatttttt attccttatt attattatta
     2341 tgtatgttaa ttaattgtat atgctggtta catctatctt taaaaacgaa taaaaagaag
     2401 attatctata ctttgatgtg caattaacaa gaaataaact taaagatcat catattaaat
     2461 atatttttgt gacaggaggt gttctttcag gattaggcaa aggagtgatt tcttcttcta
     2521 tagctaaaat tttacaatta acaggtctta atgtttcatg cgtaaaagta gacccatatg
     2581 taaactatga tgcaggaact atgaatcctg ttgctcatgg ggaggtattt gtgacatgtg
     2641 atggcgggga atgtgatatg gatataggaa attatgagcg ttttttaaat atagaactat
     2701 ctagggatca taatataaca acagggagaa tttattctga agtaataggt aatgaaaggc
     2761 aaggtaaata tctaggtcag tgtgtccaaa taattcctca tgttactgac gcaataaaaa
     2821 ataaactaag gcaaatatct attcaagaag acttagatgt attggtaatt gaatgtggag
     2881 gaactgttgg agatattgaa agtctacctt tcttagaggc ttttcgacaa atgaaactcg
     2941 aagaaggaga ttataatacg ctatttgttc atgtaacttt agcaccaatc ttggatgtag
     3001 taggagaaca aaaaactaaa ccaacacaac atagtgtaca agaacttaga aggataggta
     3061 ttaatccaga tctactagca gtaagatgta aaaatacgct atctgaggat gcaaggagaa
     3121 aaatatctct gtttgctaac ataagttcga attgtgtttt ttcttgtcac gatgttaaat
     3181 ctattttttt agtaccagaa attctggcgg atcagggaat tgttagtatg ataagtaata
     3241 aattaagatt acagaatcta tttataaaat ggtctaactg gaaactaata gcaaaatctt
     3301 ttttcgaata tagtaaagaa gtacatattg caatagttgg taaatatgtt tctcttcctg
     3361 atagctacgt aagtgtgtat cagtctcttt ctcatgcagc agctaagcta ggaaataaat
     3421 tggaagtagc atggattgat tctgaaaaat ttgatactaa ttctaaagaa aaaatttctt
     3481 tacttaaaaa atttgatggt attcttattc ctggaggatt tggaaaacgt ggttctgagg
     3541 gtataattaa tgtagctaat tttgcacgaa aggaaaatat tccctttctt ggaatatgtt
     3601 ttggatttca attgtctatt gtttcatttg cgagaaatgc gtgtggaatt gataatgcta
     3661 attctactga aatagatcca gatacctgta atcctgtagt tgaatttatg cctgaacaaa
     3721 agacgataaa tactctgggt gggacgatga gattaggtag tcataaaata ctaataaatt
     3781 caaatactct tgcgtcaaaa atatactcaa agaattttat tattaaaaga catagacaca
     3841 gatttgaatt taacaaaaat tacttacctg ttttaagtaa aaacggaatg gttttatctg
     3901 gtttttcaga caatgaaaaa cgaacagaga tattagaaat tcctaatcat aaattttatt
     3961 ttgccgttca atatcatgca gaatttgaca gtcggccggg aaaaccagag caggcattcg
     4021 aggcctttat taataatgct tccctcaaat cttgagctca tatgttctat gcctggcatc
     4081 acgtaatgat gttttctttt ttacataccc tctattaaca agatgaccta aagataatct
     4141 gattgtcctt tctggtaata atgttttatt agctaaatcc ttctggctca tagatccttc
     4201 atattccaat attttcaata ttagctttgc acttggtggc atattcaata attcttcagc
     4261 aatttttact tttttagcaa ttaacgaagt agcagaaccc tcaccaatta gtctcactag
     4321 ttttgctgga tattgatgtt tactacatgt tagaattttt ttaataccca atcttcttcc
     4381 accatctaaa actacttcgc aatgatatct acttaaaata tctgatattt ctaatatgca
     4441 attatccgaa aatattaatg gtcttctagt gttgtctaca gaattgacag atactgctac
     4501 aaaaactttt gaattctgta gtatcatagg accgcctgct gacattgaat aggctgtgga
     4561 tccagtaggg gttgatatga ttactccatc gctattgtca tgccaaacgt catatttgtc
     4621 aatacgtaat atatgttcca ttaaagtagc acttttactt ggaaatactg caacatcatt
     4681 taatacgggt tccatttcct ttccgtctac atttactgct aatctaacaa cctcatcaat
     4741 tctataatca ttttttttta gtctttgaat gtttgtttcc aaatcgcgta catctatttg
     4801 agctaaaaat ccagtagatt cagattcata tattccgata actggagcag attcatccac
     4861 tactttatga aaataatcta acatcgctcg atctccacca actataaata ctatatcgac
     4921 ctgatttgct agattaaaat tattttcagt catgataata gtttgaataa acgaattatc
     4981 aagaattttt ttaatctttg agattaataa tgtatcctga ggatgagcac caactagtgc
     5041 aattttcatt attttctata tatttatcta ttctctcatt taaagttaaa tttttttgtt
     5101 attcttatgt gaatttaaca ttttatgttt aatctcaatt tttataaata ttcgattttt
     5161 tagatctccc gtaattgaca tataattaaa gactttattt acaagagtta attttaccag
     5221 aacctttaaa gtatggattt agaatcataa ttatatgcca aagaaacgta ctagtcgtgg
     5281 ccgtaccaaa ggaggaaagg gtagttcagg aactgttcat tgtagtcagt gtggtgctat
     5341 ggtccctaga gataaagcaa aaaagattac aggcaggatc accttagttg aacctacttt
     5401 agctaaagag ttaagagccc aaggagcata tatttctcca gcgactgatg tcaaatttta
     5461 ttgtgtttct tgtgctgttc atagaggaat agtcaaggtt agatctgaag tagaacgaag
     5521 agtgaataca agacttagat gatcattcta tcatttttaa tgtcttatat attctctgta
     5581 taaccgtgat aatagcaatt cctatcacta ttataacacc atattctaat gaatgagaaa
     5641 aaggaagtaa tcccattata gcaagaatca gtattctttc tgctctttct ccaaatccta
     5701 ctccgcttaa atcaattcct aatgtttctg cacgcgatct agtatagctg accaacaatg
     5761 atgcggatac tgctattata cacaatattg gattagccaa ttctcctatc gctattccca
     5821 aaaatatgat tgcctcagaa atcttgtcta atgtagaatc aagaaaagag ccttttctag
     5881 atgttctctt agtaatttta gctacgctac catcaatcac atcaaagaac ccaccagtta
     5941 acaaaagaag actacctatt gctgagaaat ttactagtgg atctagttgt aaggaattaa
     6001 gtccaaaaat aatagaagaa ataatagtta taatgaatcc aattaaagat aatgtatttg
     6061 gtgataaccc aaaagatgaa aataaaactc cggttttatc caaaattggt tgtaaatgtt
     6121 tcctaaattt gtttagcaat aatctaaacc attaatttta attattatct ctttatattt
     6181 ttaacattta aacttttgat tgatataatt agtaaaataa aaatttaatt actttttatt
     6241 aaaaaaactt gtatatgagt gattttgaca ggatattagt ttttggcgct catcctgatg
     6301 acatagaaat cggtatgggt ggtactatta caaaactagt aaagcttggt catgatgtaa
     6361 tcatggttat agctacacta ccaaatttta ctattgctga taataaagat gaaagaaaat
     6421 tggaagctat atcttctgct actattttag gttgtaactc tcctgaattt ttagatttag
     6481 ctcaggaaga tatagtgttt ggtagaagaa tggtaacatt agttgattct ttaataaaga
     6541 aatatgatac aagtacagtt tatactcagt ggataggtga ctctcatcaa gatcatcaaa
     6601 ttcttaccca gtctgttatt gctgcctcac gtgatcttaa caatttgtat atgtatgaaa
     6661 caacaatacc tggaggctta acagaaaaag cctttaggcc acagctatat gttgatatta
     6721 ctaaagaaat ggaaaataaa acaaaatctt tgaaatgttt taaatcacag aaccttagat
     6781 gcgggccttt ttggatagat gctattgttg gaagatctat atatcgtgga tatcaaatga
     6841 acacaaaata tgctgaggca tttgaaattg tgaaaactgt tcaattttag ataataaaaa
     6901 aaggtattgc tgatgattat tgttctcctt ttttagacag ttttttataa aaaagcagaa
     6961 acaatgcttg tccagtcaga gcaataacca cggcaagtgc gatacctgta acttcaatca
     7021 atcgtcctag caaaatcatt aaggtaagtt gtaggatttg ataaataatt gctccaataa
     7081 ctactccttt tgtattacct tcgcttaaca attttgaatt aataacatat gctatcatca
     7141 ttggaattat tccaatgcta attactctta tagcatctaa tgaattctga taatgaggaa
     7201 aaaatacatt tatgataatt ggactcaaaa aatataataa agctgctaga cctatagata
     7261 atccgaatcc tattattctt aactttgttt ttttgtttcc acttgattcc tctggaataa
     7321 gatattggaa aaagctaatt ggtaccattc caagaaacat caaaaattga aaacctaatt
     7381 gataataccc caaaatagca tatccaaata ttggggggat aataatttta tcaaagtaca
     7441 ttaataatgc atttgacata ttaaacgaat aagtatgtaa agcaaatttc atttttccac
     7501 taatctcact aaagtctttt ttaaaatatt tgattgaaag aaaatatctg tgactaaaca
     7561 agaaaaaaga gattgtaaag ccaattataa ttccaggaat accaattata taataaagtg
     7621 cgatggaaag taaaagttga caacctcttg ctccaatatt cgcaattgcg tattgtttat
     7681 aggattttct tccgagaaat tcgtatatac tcatcatcca aaaactcatt ccaactacga
     7741 aaaaacctaa taaccaatct ctttgtgcta caattaaagc agcaataata cttgaaatta
     7801 atacaacctg gtttgctgca acatgaattt tatcatgtcc tttaggtatg aaagttgtaa
     7861 tagctgtatt caaacccaaa agagcaaaac tggaggccaa ggaagccata gaaatcatat
     7921 aactcgtttt tccatattct tctacactct gcaatgatgc tagcaaaagc caaaacccgc
     7981 cagttaataa agcaccaaca atattgccaa gtatgatata tactagacct ttgttagatt
     8041 ttagaaactt gagcaaatta tattcacgtt atcttttatc tataaaacaa gacctatcaa
     8101 taaaatggtt tatgtctttt aatagagtat tttcataaga aaaaatagtt ataagatttt
     8161 taatgtataa tataacagaa ctcgcttggt ttatacgtat ttatatattt ctagattatt
     8221 atctgataaa aagaataata actatgtaat atatcataaa catgattaat atttataaaa
     8281 aaagctttag tttgttataa atatctagaa acctttttca tataaatgaa tgagaataat
     8341 ttagaaaaac taacttatct aattaatata tctattaata gtattgtaaa tgtaaaagat
     8401 caccgtagcg aatcttaggg gtatttttaa atagtagaat aaatgtaaaa aaaatctagg
     8461 agcaaattat atgaaaaatt ccgatagaaa atttcagttg ttatcggctg tactaattac
     8521 tcttttctgt tgtattttat taattaactc taattatgta ttagttgaat ctcaaacttc
     8581 acccacattt cttaccctta ctggttctaa ttttttagat gttgaaaata gcgaaagatt
     8641 acaattaaaa gactttacat tgtctacgtg gtttagaaca gataagcagg attacttaga
     8701 acccggcacg attgttaata aaggtggaat gaacgacgat gccccgggaa aaaatatgaa
     8761 ttatggtatt tggattactt cagaagaatt tcttcaagga ggatttgaaa ccaaaacagg
     8821 aaccaatatt ttcgttacct ctaaacaaaa atttaacgat ggaaattggc ataatgttat
     8881 actcactaac gatggattat ttttgaaatt atatgtagat ggagtggaaa ttggcaaaat
     8941 atctacatta ggtgcaatac ctgatatgaa aggatcgcaa tctataagat taggcgcaaa
     9001 tacacttaat cttgacaaat tttttactgg agatatagat gaggtaattc tttgggaccg
     9061 tgctttgtcg ttagaagaaa tttttaattt ttataattat gatgaaatac catcaaatgg
     9121 ccgaatctta tatcttcctt tttcatccat ttctcccatt cagtcatctt acaaatacca
     9181 accgtttaaa acatttgaac aaactgaaaa agaagtcatc gatggagaag agtcattccg
     9241 cctaagtaat ttctcacttg gtacgtggtt tagaattgat agaatagacc atactggacc
     9301 tggaattcta ttaaataaag gtggttttgg gggggaacgt cctggcgaga atcttaactt
     9361 cggtatatgg ttgaatccgt cagagaccat agaaggagga tttgaaacta aacccggaac
     9421 taatgtattt gtatcatcac aaggaaaata taatgatggt ctttggcatt atgttttatt
     9481 aacatatgac aataaaattt taaaactcta tattgacggc gttctagcag atagtaaaat
     9541 tacttctggc gcaattcctg ataatagcgg agaccaagac tttgtaatcg gtggaaattc
     9601 ttttctggat gacaagttct tcaaaggaga tcttgatgag attagagtat ggaatagagc
     9661 acttaataca gaagacgtat ctaatggatt cgacaaaaac actttcaata caaatggaca
     9721 gattttttat gagtcatttt ctgatttaga cacacaacca ccatcaccac cacctactga
     9781 aactccaggc acaacaccac cacctactga aactccaggc acaacaccac cacctactga
     9841 aactccaggc acaacaccac c
//
LOCUS       DASVEV010000068        14271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1185220, whole genome shotgun sequence.
ACCESSION   DASVEV010000068 DASVEV010000000
VERSION     DASVEV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185220"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1352)
                     /locus_tag="VF222_03415"
     CDS             complement(282..1352)
                     /locus_tag="VF222_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HEX7141507.1"
                     /translation="MEKEKEKNKELIIAPLSNVSNLELFLDQLDENDTLFIDPTKIKR
                     KKNFKIIYESELADIIICKSMEDLITKKTKGKSLAYYKEVFSNEDISDLENAIYNGAN
                     YIIVDAPNWKIIPLENIIANLQNRRAKIFTTAKNSEEVRTMFAVLELGVDGVILSTAN
                     VKEIEESKQYLQDKKFEIKVCEIVDFRDIGRGERVCVDTVSMLETGEGMLVGSKSNFL
                     FLLHNESAGSSFTSPRPFRVNAGAVYCYTILPNGKTTYLSELESGTEIYIVNKEGKSR
                     LVSVGRSKIETRPLRLIKAIGNNTEGTVIVQNAETIQLIRDTGELLPVTSIKQGDKIL
                     GYFKPSSGRHFGVEVKEYILEK"
     gene            complement(1327..2127)
                     /locus_tag="VF222_03420"
     CDS             complement(1327..2127)
                     /locus_tag="VF222_03420"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HEX7141508.1"
                     /translation="MVSGRDIRLNRITKKGKMVCIPMDHGITNGPIKGLENPHEMIYQ
                     CENSGLTAVIINKGIIKTLPRTTTIGLITHLSASTSLSPYPNKKVLIGNVADAIRLGS
                     DAVSVHINIGAKEEPEMLEILGMVSDECDEWDVPLLAMMYPRGENIKNPYDPEIVAHV
                     TRIGAEAGADIVKSVYTGDVDSFKSVVKRCPVPIVIAGGPKSKTDTDILEMCSNAIEA
                     GAKGVTFGRNIFQHDTPSSLIKALCEIVIDRKNYSEVLKNFGKRKRKK"
     gene            complement(2229..3005)
                     /locus_tag="VF222_03425"
     CDS             complement(2229..3005)
                     /locus_tag="VF222_03425"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7141509.1"
                     /translation="MNILDIANCIIEFIRLEVSRAQSQGVVLGLSGGIDSSVAVSLAT
                     KALGNNRVLGLILPDRNVSEQKDIDDAVELSKQLGIKYHIIDITEIKQKYLDLLPLEK
                     KSIGNLVARMRMTLIYYYANLFNKLVLGTSDKSEMMIGYFTKFGDGAADLLPLADLYK
                     TEVRELGIYLNVPKTILLKKSTPSLWLNQTAEKEIGLEYKKIDAILKYLDENNSARTE
                     KDLLKKGCNKKDVVFVKDRLLKNIHKRISPKICVISSNNS"
     gene            3158..4222
                     /gene="purM"
                     /locus_tag="VF222_03430"
     CDS             3158..4222
                     /gene="purM"
                     /locus_tag="VF222_03430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7141510.1"
                     /translation="MSNSNINTYDKAGVNIRKIRSTQRNIEKMITKSYSFQEIGKITW
                     GFGHYSGLITIGKNVLALHTDGVGTKVLVAQQLKCFDTIGIDCIAMNVNDIICTGAEP
                     FAFVDYIGLKKVNDELVKKVMEGLIAGAKAAKIAIVGGETAVVPELLAGESEEMFDLA
                     GTALGMTQNKKLILGNEIKIGDIILGLESSGLHSNGYTLARKVLSKYSLDKIPEFVTK
                     SIGEELLTPTRIYVQPIMKLIKDRKITVHGLAHITGGSFTKLKRLNNKVRYNLSHLSP
                     PDGIFKQIQKDGTIDLKEMYRTFNMGIGFCIILPKANVDKTISIIEKYNMKVSQIGEV
                     DSKGNGNVIGKVENKKYIFT"
     gene            complement(4495..6105)
                     /gene="lysS"
                     /locus_tag="VF222_03435"
     CDS             complement(4495..6105)
                     /gene="lysS"
                     /locus_tag="VF222_03435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7141511.1"
                     /translation="MIENIIGKGTWLDKVANNLIIREKKLNRNIDSIKVESGLGASGI
                     PHIGSMGDAVRAYGISLALQNLGYNSELIAYSDDMDGLRKIPSGFPPWLKDHIAKPVS
                     QIPDPFGDCHNSYGSHMSNLLLDGLDSVSIKYVFKSATDVYKSGILIDAIDQILTKSE
                     YLGQKISEFVGQNKFLKVLPYFPICKSCGKLYVAHAYEYLTKNKKVLYECSDSKIGNK
                     EIKGCGYKGEANIGKAEGKLAWKVEFAARWKIFDVRFEAYGKDIMDSVKINDWICEEI
                     LNFPHPLHIKYEMFLDKGGKKISKSAGNVLTPQKWMRYGTPQSILLLLFKRITGTRHV
                     GIDDVPSLMDEYDLYEDLYFGKLKETNQSKMIKLRGIYEYINHLNPPNNNSHHVPYRL
                     LVQQTSLFEGEDRVGKVYERLKKYRLVNEKSNDIIQKIQLAFNWSNDILVNENSQNIS
                     LEIEQKKALRELVSNLRSYVTNENNDMANDIQTLIYETSKKNEIEPKNFFKLLYQILI
                     NSDRGPKLGNYLVDLGIARTCEIIEKYL"
     gene            complement(6930..8525)
                     /locus_tag="VF222_03440"
     CDS             complement(6930..8525)
                     /locus_tag="VF222_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HEX7141512.1"
                     /translation="MNSICYSEACKSIAMELENHNDMSLKEINKIISLTCSAYKLSVV
                     PKKEHIIPYLSRNSNYREVLMVKPAKTASGVAVIAVMPKPYDCPHGKCVYCPGGLEYN
                     TPMSYVGTEPATKIAQSFKYDAYYQVKSKLNQLYQRGHNITKIELVIVGGTFPFMPES
                     YQREFVKKCFDALNDDLSSITLEESCRKNEYGTIRCVGFTVETKPDYCKEKHLDLMLE
                     LGITRIEIGVQTLQENIYKLINRGHNLKDIKNSFQIARNCGYKIVAHMMPGLPGSTPE
                     SDISGFKILLENDSFKPDMLKIYPTLVLKNTGLYKLYMDGKYDSYTEDDIVNILVEVK
                     KLIPPWVRIMRIQREIETSDIVAGAKKGNIRQIAIKKLKDLGLKCNCIRCREAGLQKL
                     KNLNQQEITLNRIDYIASKGKEVFLSMENKDKNLLFGFLRLRNIPFSHRKELCETHYR
                     GSAIIRELHVYGPVVDVGQENRSLSFQHSGLGLSLMEEAEKISKDEFQVDKLSVISAI
                     GTRAYYRKLGYLQNGPYVTKELK"
     gene            8746..9690
                     /locus_tag="VF222_03445"
     CDS             8746..9690
                     /locus_tag="VF222_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX7141513.1"
                     /translation="MRVTLFSHESDVDGIFSTSIGLIRYPQARTFFLDYSKESFMKIV
                     NQIFFSNESQEKNIFIVSDLGFNDSLLSIFKSAFLGSQKSNNEVIWVDHHPWSKNTID
                     EVNPFVELILDNSGIKCASELMYERFLFDNGYAKHLANLAHTIDFFTKDQYLTPLPEI
                     IRYYLNFNDSYERLAKLAHKISKGILWDIDLSEDYRIYESLRDIDKRQVLNDLKIKKM
                     DDLNVVFVRSSPYIQTSIFSEEVFNLTNADVAIFYGKNGKISIRRNNDKIDCNAIALN
                     LIDGGGHKFAAGGTIRSDPNKLEDIILEIEQTIHKSKL"
     gene            9754..10383
                     /locus_tag="VF222_03450"
     CDS             9754..10383
                     /locus_tag="VF222_03450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7141514.1"
                     /translation="MIIFDGKIEEDLIKCKAIIKNGGIIVYPTDTIYGLGCDPYNKKS
                     VEKIFTIKKRLLNKALPILTFDIRHVEKIMFLDDRGRKLADSFWPGPLTIIGILKDND
                     IPKLITAGRQTVGMRIPNNIETLNLLKYCNFLVGTSANISNQNSCIYVEDVLKSPLVG
                     YDAIVIGDDDKPKEPFSVKGSTIVDISASNLKIVREGVIKSETIYEILY"
     gene            10407..10943
                     /locus_tag="VF222_03455"
     CDS             10407..10943
                     /locus_tag="VF222_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7141515.1"
                     /translation="MITTYRHREFDAVDELTLTLQRFGVDAPMIEIQKVPGIILALTN
                     LNPFNLIRNFKELLAEEPWQFRYILRVIPIEKSLYTDLKNIEIEVENLATKKIKVNDS
                     FRISVEKRHTPMRSKEIISSTADMLTRFKVSLENPDWIILIEIIGNNTGISVLKDIDI
                     FNSIMEKRKAGHLLESSG"
     gene            11083..12021
                     /gene="pyrB"
                     /locus_tag="VF222_03460"
     CDS             11083..12021
                     /gene="pyrB"
                     /locus_tag="VF222_03460"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX7141516.1"
                     /translation="MPSKNHFFGKDIVSIRDVSREDLEFIFDSTDKIQQLKPEEKSEL
                     GRGRTLGYIFYEPSTRTRMSFEAAMASLGGSSIGIFDSKFSSIEKGESLADTIRIMDL
                     YSDVIVLRHARDGSSRYAAELSSNPIINAGSGSEEHPTQAMLDLYTIFKEKGRIDNLT
                     IGIVGDLKYGRTVYSLLYGLKNYHVNVHLISPPSLAIRKESIFEFEGIMNIKHYDALG
                     DIIPKLDVLYVTRIQKERFPDTQEYEKVKGTYRIDEKILDNGKTDLSIMHPLPRLDEI
                     SHTIDYTKNAIYFKQAAYGKELRSALLALILNEDPF"
     gene            complement(12126..>14271)
                     /locus_tag="VF222_03465"
     CDS             complement(12126..>14271)
                     /locus_tag="VF222_03465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX7141517.1"
                     /translation="KISDFDLKLKIGSYYLNKYHFESLIRLYTQLASKGQMKTSFKDI
                     LKLIFQGKFTSYEDRIKEYYNLMISKDFLPNTPTLMNAGARLGQLSACFVLDMPDDME
                     KIMKTSSDAAMIFKSGGGVGINYSDLRPEGDIVASTSGVASGPASFMRIIDTITDVVK
                     QGGKRRGANMGILEVWHPDIEKFITAKTKPGVFENFNVSVGLWDDFWKALINNNNKYT
                     LRNPRTHEPLKQIDSHQLIDLISLSAWKSAEPGIIFFDNINKYNPCIKAKEGPLRATN
                     PCGEQSLYPYESCNLGSINLSNFVKRKADGLYEFDWPRYEQAIRISSRFLDNIIDANK
                     YPVDEIDYNTKLTRRIGLGIMGIADLLFSLRIPYASQEGYEFMNKLAETITYYSMEES
                     VAIAKSRGSFPLFKKSEYVLEKIPVAGYYEIPKQKHSYDWDSLIEKIQKYGIRNSWTT
                     TIAPTGTLSMIADCSNGVEPVFALVFEKRVTVGRFFYTNKIFENILKENGLYNDDILT
                     KIANNYGSVRGLNEIPPWMQSLFTTAIDIHWTDHIMAQSVWQKWISNAIAKTINMPGD
                     VTVEDVKSSYLLAHELGLKGVTVYRDGSRNEQVLHITGTNTLEKSFIVKPSKYVKEYI
                     QSQIKEPYVTQYVSKLFDTKDIEVTDDYVVDDIPIPTRESTKETEHIEFIQKPLPEIF
                     EDEICPSCNSKLIITEGCNMCIECGFSSCISG"
BASE COUNT         4854 a         2245 c         2066 g         5106 t
ORIGIN      
        1 ctataatcga ttgcatttct atatggagga gatgttattg ttaactgaat tgaatcatta
       61 ggaattttag aaattatttc tttgcaatcg cctgataaaa ttttatcaac ataactacgc
      121 tttattttca taatggaaaa taaattttta atcaatattc agcatgcttt aaaatattcg
      181 actaactaat aaagaaattt tatacaataa caattgaact tattagagat gaaacaagta
      241 ttttgtacta aacaaactaa atgagtccaa aaaaaaatta ttcatttttc aagaatgtat
      301 tctttaactt cgaccccaaa atgtcttcca ctactaggtt taaagtatcc taaaatttta
      361 tctccttgct taatggaagt aactggtaat aattctccag tgtctcggat taattgaatt
      421 gtttctgcat tttggactat aacggttcct tcagtgttat taccaattgc cttaattaac
      481 ctcaatggtc tagtctcgat tttagacctc cctacagata caagtctaga ctttccttcc
      541 ttatttacaa tatatatctc agtcccagat tctaattcgg aaagatatgt agttttccca
      601 ttaggaagaa tagtgtaaca atataccgct ccagcattga ctctaaaagg acgaggagaa
      661 gtaaaagagg agcctgctga ttcattatgt aaaagaaata gaaaattgga tttacttcca
      721 actaacatcc cttctcctgt ttcaagcatt gatacagtat ccacacaaac tctttcccct
      781 ctacctatat ctctgaaatc tacaatttca catactttta tctcaaattt tttatcttgt
      841 agatattgtt tagattcttc tatttcttta acatttgctg ttgaaagtat taccccatct
      901 acacccaatt ctaacacagc aaacatagtt cttacttctt cagaattttt agcagtggta
      961 aagattttcg ctcttctatt ttgtagatta gcgattatat tttccaatgg aataatcttc
     1021 caattaggag catctacgat aatataatta gcaccattat agattgcatt ttctagatca
     1081 gaaatatctt cgtttgagaa aacttcctta taataagcca aggatttacc ttttgtcttt
     1141 tttgtaatta aatcctccat agatttgcaa atgataatat cggcaagttc cgattcataa
     1201 attattttaa aatttttctt tctttttatt ttagtagggt ctataaataa cgtatcattt
     1261 tcatctaact gatccaaaaa taattcaagg ttagaaacat ttgaaagagg agcaataatt
     1321 aattctttat ttttttcttt ttctttttcc aaagtttttc aatacctcac tataattttt
     1381 tctatctata actatttcac ataatgcttt tatgagagaa gaaggagtat catgttggaa
     1441 aatattcctt ccaaaagtaa ctcctttagc acctgcttca atagcattgg agcacatttc
     1501 taaaatgtca gtgtcagttt tagattttgg accacctgca attacaatag gaacaggaca
     1561 cctttttacc actgatttaa aagaatctac atctccagta tacacagatt ttactatatc
     1621 tgctcctgct tctgcaccaa ttctagtaac atgtgctact atttccggat catatggatt
     1681 ttttatgttt tcccctcttg gatacatcat agctaataat gggacatccc attcatcaca
     1741 ctcatctgaa accatcccca agatctctaa catttcaggt tcttcttttg cgcctatatt
     1801 tatatgaaca gaaacagcat cggatccaag tctaatagca tccgcaacat ttccaataag
     1861 cactttttta ttcggatatg gacttaaaga agtgctagca gataagtgtg ttattaatcc
     1921 tatagtagta gttctgggca aagttttaat aatccctttg ttaataatta ctgctgttaa
     1981 cccagaattt tcacattgat atatcatttc atgaggattt tctagacctt ttattggacc
     2041 gttggttatt ccatggtcca taggaataca aaccattttg cctttttttg taattctgtt
     2101 taacctaatg tctcttcctg agaccataaa gatgaaggat atttgtccta cttaaaagtg
     2161 atctgctagg tgtaaacagt cgtaaataag aaaataaaag gaataatgat aattagattt
     2221 tgattcactt aagaattgtt agaggatata acacatattt ttggtgagat tcttttatga
     2281 atatttttta ataaccgatc tttaacaaat actacatctt ttttattaca tccttttttt
     2341 agtagatctt tttctgttct agcggaatta ttttcatcga gatacttcaa aatagcatca
     2401 attttcttat actccaatcc aatttccttt tctgcagttt ggttgagcca taaagaagga
     2461 gtactttttt ttaataatat agtttttgga acatttagat atatacctaa ttctcttact
     2521 tcagttttat ataagtccgc taacggcaga agatcagccg caccatctcc aaatttcgta
     2581 aaatatccaa tcatcatttc acttttatcc gaggtcccta aaactaattt gttaaagaga
     2641 tttgcgtagt aataaattaa agtcatgcgc attctggcaa caaggttacc tatcgatttt
     2701 ttttctaatg gcaatagatc taggtacttc tgtttaattt ctgtaatatc tattatatga
     2761 tattttatac ccaattgttt ggataattca acagcatcgt ctatatcctt ctgctctgag
     2821 acgtttctat caggtaaaat taatcctagt actctattat tacccaatgc tttagtagct
     2881 aaagatactg caacagaaga gtcaattcct ccacttaaac ccaatactac tccttgagat
     2941 tgagctctgg agacctcgag tctaataaat tcaataatac aatttgcaat atctaaaata
     3001 ttcaagattt catttattat taaattaaat attactatta aatcacactt ttattattta
     3061 taaattttaa agttttcaga taactaaaaa gttagatatc atatattaaa tcccaaaaaa
     3121 gttttattag ctataaattt gctgaaataa ttatattttg tctaactcaa atattaatac
     3181 ttatgataaa gcaggagtaa atattcgaaa gattcgttct acacaacgaa atattgaaaa
     3241 aatgattact aagtcctatt cttttcaaga aattggaaaa attacttggg gatttggcca
     3301 ttattctgga ttaataacta taggaaagaa tgttctggca ttacatactg atggtgttgg
     3361 aactaaagtt cttgttgccc aacagctgaa atgttttgat acaataggca tagattgcat
     3421 agccatgaat gttaatgata ttatttgtac gggagcagaa ccgttcgcat ttgtagacta
     3481 cataggttta aaaaaagtta acgatgaatt ggtaaaaaaa gttatggaag gtttgattgc
     3541 aggtgcaaaa gcagcaaaaa tagctattgt tggaggtgaa actgctgtag ttcctgaatt
     3601 gttagctggt gaatcagagg aaatgtttga cctagctggc actgcactag gaatgacaca
     3661 aaataagaaa cttattctag ggaacgagat caaaataggt gacatcatat taggattgga
     3721 aagtagtggt ttgcattcaa atggctatac actagcacga aaggtattat caaaatattc
     3781 cctagataaa attcctgaat ttgtaacaaa atcaattgga gaagaattac tcactccaac
     3841 tagaatttat gtacaaccaa taatgaaatt gataaaagat agaaaaataa cagtacatgg
     3901 tttagcgcat ataacaggag gatcattcac aaaattaaaa agattaaata ataaagtaag
     3961 atataacctt tcacatttat ctcctcctga tggtattttc aagcaaattc aaaaagatgg
     4021 cacaatcgat cttaaagaga tgtatagaac ttttaatatg ggaatcggtt tttgtattat
     4081 acttccaaag gcgaatgttg ataagacaat tagcataata gaaaaataca atatgaaagt
     4141 ctctcaaatt ggagaagtgg attcaaaagg taatggaaac gtaattggga aagttgaaaa
     4201 taagaaatat atattcacat aacttcaata gtaattctct atatttagtt aatttattaa
     4261 cctaagtaat atcaaatttt attaggtatc ttataaccag attatttgaa ttttgtaaag
     4321 cattgagttt ttatgtgttc catatatgat tgccgttgtt attataatca catgtattga
     4381 acatggtaat aaaattcata accactattt gatatttaaa cttaaaattt tcattattta
     4441 atcaaattaa ttcttattta ttgtaaaata agaaaaatat ttaaaaagaa aatatcaaag
     4501 atatttttca attatttcac aggttctagc aattcctaga tcaaccaaat aatttccaag
     4561 ttttgggccc cgatcagaat taattagaat ctggtagagt aatttaaaaa agtttttcgg
     4621 ttctatttca tttttttttg aagtttcata aattaatgtt tgaatatcgt ttgccatatc
     4681 attattttca tttgttacat atgaccttag atttgatact aattccctca atgctttctt
     4741 ttgttctatc tccaaagata tattctgact attttcattt actaaaatat catttgacca
     4801 attaaaggca agttgaattt tctgtattat atcatttgat ttttcattaa ctagtctata
     4861 ttttttaagt ctttcgtata ccttacctac ccgatcttca ccttcgaata gggatgtttg
     4921 ttggacaagt aatctatatg gaacatggtg agaattatta tttggcggat taaggtggtt
     4981 tatatattca taaatccccc gtaacttaat catttttgat tgattggttt cttttaattt
     5041 accaaaatac aagtcttcgt aaagatcata ttcatccatc aatgaaggta catcatctat
     5101 tccaacatgt cttgttccag ttattcgttt aaacaataat aaaaggatgg attgaggtgt
     5161 accatacctc atccactttt ggggcgttaa aacattccca gcagatttac ttattttttt
     5221 gcctcctttg tctaaaaaca tctcatattt tatatgcaaa ggatgtggaa agtttagtat
     5281 ttcttcacat atccaatcat ttattttcac agaatccata atatctttac cgtatgcttc
     5341 aaatcgaacg tcgaatatct tccatcgtgc agcaaattca accttccagg caagttttcc
     5401 ttcagccttt ccaatgttag cttctccctt atatccacac cctttaattt ctttattacc
     5461 tattttgctg tccgaacatt cgtataatac ttttttattt ttggtcaaat attcgtaggc
     5521 atgggctaca taaagttttc cacagctttt acaaatagga aaatagggta atacctttaa
     5581 gaacttattt tggcctacaa attctgaaat tttttggcca agatattcac tctttgtaag
     5641 aatttgatca attgcatcaa ttaatatacc acttttatag acgtctgtag cacttttaaa
     5701 tacatattta atacttacac tatccaatcc atctagaagt aaattactca tatgagaacc
     5761 atatgaatta tgacaatcac cgaatggatc tgggatttgt gaaacaggtt tcgctatatg
     5821 atctttgagc catggaggaa agccagatgg gatctttctt aatccatcca tatcatcaga
     5881 ataagcaatt aattcagaat tatatcctaa attttgtaat gctaaactaa tgccataagc
     5941 ccggactgca tctcccatac tgccaatatg aggtattccc gaagctccta atccgctttc
     6001 tacttttatt gaatcgatat ttcggtttaa ttttttttcc ctaatgataa gattatttgc
     6061 tactttatct agccaagttc cttttccaat aatattttct atcattttac catacctttt
     6121 tattatttga acaaatttga tttgttataa tgctaaagaa tacatctact tttaatccta
     6181 ttgtatacgt agttcaaaat ctagcaatat aattttatta gaccaatggt attcttaaat
     6241 atcaaaatta atataataga agctatctaa tttagtttta ttatttaata atatagtgct
     6301 attattatta atttttggat aggagcatat agattaggat acgtaattgt caacatttgg
     6361 taattacgaa gtcatataaa acgtggtcta tttgtatttc gcatttgaga attgtcatag
     6421 tctattaagt aatgtctttt attttttata ttttcttttc ttgttcaaca tttattcttg
     6481 gtaagaatat tttagaataa cgcaaatata aaaaatagat gtaaagtaaa ttatcataac
     6541 aagaagtaga atatattagc ttcaaatttg aatattttag acacattaaa aaataataaa
     6601 ctttacctta ttcatcctaa taaattatcg aaatgtactt tttattggat ttagagctgg
     6661 tattctttaa cttaccatgt ttttccaatt aatcttttct ttttcaaatg taatatctta
     6721 ttggatagat aaacttacag ttagggattg atgcttttta ctatcacaat aaataacaat
     6781 atacttccct tgatatgaaa tcaataaagc ctatcgggaa acatgtcgtg ttaataaaga
     6841 aaagacaatt atgctataat atttttgctt caacagcaac ttctacataa taaaaacttt
     6901 aggatagaga tcttatggta tgcatttatt tacttcaatt cctttgtaac atagggaccg
     6961 ttttgaagat atcctaattt cctataataa gctcgtgttc caattgcact gataacagat
     7021 agtttatcaa cctgaaattc atctttcgaa attttttctg cttcttccat taaactcaaa
     7081 ccaaggccac tatgttggaa cgataaagac ctattttctt gtccaacatc taccactggg
     7141 ccatatacat gtaattctct aatgatagca gaacctctat aatgggtttc acataattcc
     7201 tttctatgag aaaatggaat atttctcaat ctcagaaatc caaacaataa atttttatct
     7261 ttattttcca tagacaaaaa aacttctttt ccttttgatg caatgtaatc aattctattg
     7321 agtgttattt cctgttgatt aagattttta agtttttgga gtcctgcttc gcggcatctt
     7381 atgcaattac attttaatcc aaggtctttt aattttttta tagctatttg tcgtatattc
     7441 cctttttttg ctccagctac aatatcggaa gtttcaattt ctctttgaat tctcattatt
     7501 ctaacccaag gtggtataag cttttttact tcaacaagaa tatttactat gtcatcttcg
     7561 gtataagagt catattttcc atccatatat aatttatata atccagtatt ttttagaact
     7621 agagttggat atattttaag catatcgggt ttgaatgaat cattttctaa caatatttta
     7681 aatccactaa tatcactttc tggtgtagag ccaggcagac caggcatcat atgtgctaca
     7741 attttgtatc cacaattcct tgcaatttgg aaggaatttt taatatcttt taaattgtgt
     7801 cctcggttga tcaatttata aatattctcc tgtagtgttt ggacacctat ttcaattctc
     7861 gtaattccta gttctaacat caaatctaaa tgtttttctt tacaataatc aggttttgtt
     7921 tcaactgtaa atcctacaca gcgaatcgtg ccatattcat tctttctaca ggattcctct
     7981 agagtaatgg aagagagatc atcatttaaa gcatcaaaac attttttaac gaactctctt
     8041 tgataacttt ccggcatgaa gggaaacgtt ccacctacaa ttactagttc aattttagta
     8101 atattatgac ctctttgata tagttgatta agttttgact taacttgata atatgcatca
     8161 tatttaaaag actgagctat ttttgttgca ggctcggtcc caacataact cattggagta
     8221 ttatattcta gtccgccagg acaataaaca cacttaccat gaggacaatc atacggtttt
     8281 ggcataactg caattactgc aacgccagac gccgttttag ccggtttaac catgagaacc
     8341 tccctgtaat tactatttct tgaaagatag ggtataatat gctctttctt tggtactaca
     8401 gacaatttat aagcagaaca agttaaacta attattttgt tgatttcttt gagagacata
     8461 tcattatggt tttccaattc catagcgata gatttacatg cttcagaata acaaatagaa
     8521 ttcataataa tgactcatta aactatctac aaagggagat ttaaaaatat ttattattat
     8581 aagtaatgta ttttttataa tgatattcgc ccattcttat gatcacctta acaatagatg
     8641 aataaaaaat ggatatcata ctaacttaac aagagtggaa atgattttga tactaatgct
     8701 ttaatataat caagacttat aagaaaataa aagtaataca aactattgag agttacatta
     8761 ttttctcatg aatcggatgt tgatggtatt ttttctacgt ctataggttt aattcgctat
     8821 cctcaagcta gaactttttt tttagactat agcaaagaaa gttttatgaa aatagttaat
     8881 caaatttttt tttcaaatga gtctcaagag aaaaatattt ttatagtttc agatctcggt
     8941 tttaatgata gtttgttaag tatttttaaa agtgcgtttc tcggttccca aaaatcaaac
     9001 aatgaagtta tttgggtcga tcatcatcca tggtctaaaa atacaataga tgaagttaat
     9061 ccttttgttg agttaatatt ggataattca ggaatcaaat gtgcatcaga attaatgtac
     9121 gaacgatttt tattcgataa tgggtatgct aaacatttag ctaatttagc acacacaata
     9181 gattttttta ccaaagacca gtatcttact ccgttaccag aaattataag atattattta
     9241 aattttaacg actcctatga aagattagca aaacttgctc ataagatatc gaaaggaata
     9301 ttatgggata ttgatttgag tgaagattat agaatatatg aatccctgag agacatagat
     9361 aaaaggcaag tattaaatga cctaaaaata aagaaaatgg atgatttgaa cgttgtattt
     9421 gttagatcat ccccatatat tcagacaagc atattttcag aagaggtatt caatttgaca
     9481 aatgcagatg tagctatttt ctatggtaaa aatggtaaaa taagtattag aagaaataat
     9541 gacaaaattg attgtaatgc aattgcttta aatttaattg atggtggagg tcataaattt
     9601 gctgcaggag gaactatacg aagtgatcca aacaaattag aagacatcat tttagaaatt
     9661 gaacaaacaa ttcataaatc aaaattataa aataataatt aaatataaaa taagatttta
     9721 atcagatatt aaatataaaa tagaagaaat aaaatgataa tattcgatgg taaaatagaa
     9781 gaggatctaa tcaaatgtaa agcaataatt aaaaatggag gaataattgt ctatcctaca
     9841 gatactattt atggtttagg ttgcgaccct tataacaaga aatcagtaga aaaaattttc
     9901 acaataaaaa aacgtttact gaataaagct ttacctattt taacctttga cattagacac
     9961 gtagaaaaaa taatgttttt agatgaccgt ggaagaaaat tagccgattc tttttggcct
    10021 ggtcctttaa caatcatagg gatattaaaa gataatgaca ttccaaaatt aattactgca
    10081 ggtcgacaaa cagttggtat gaggattccc aataacatag aaaccctaaa tttattaaaa
    10141 tattgcaact ttctagttgg taccagtgct aatatatcaa atcaaaattc ttgtatttat
    10201 gttgaggatg tactgaaatc tccattggtc ggctatgatg ctatagtaat tggagatgat
    10261 gataaaccta aggaaccctt ttctgttaaa ggatcaacaa tagttgatat ttctgcttca
    10321 aatctcaaga tcgttagaga aggagtgata aaatcggaaa ctatttacga aattttatat
    10381 taataaaaat aatggatttt aatttattga tcactactta cagacataga gagtttgatg
    10441 ctgttgatga actaactcta acactccaac gctttggtgt tgatgcgccc atgatagaaa
    10501 ttcaaaaggt tccaggtata atacttgcat taactaattt aaatccattt aacttaataa
    10561 gaaattttaa ggaattattg gcagaagaac catggcaatt tagatatatc ctacgagtga
    10621 tacctataga gaagtctctt tatacagatc ttaaaaatat tgaaattgaa gtagaaaatc
    10681 ttgcaacaaa aaaaataaaa gtaaatgatt cattcagaat ctcagtagaa aaaagacata
    10741 caccaatgag aagtaaagag attatatcat ctactgccga tatgcttact agatttaaag
    10801 tttcattaga gaatcctgat tggattatat taattgaaat cattggtaat aatactggaa
    10861 tttcagtatt gaaggacata gatattttta attcaataat ggaaaaaaga aaggcagggc
    10921 atttattaga atcgtccggc taattgtcca gggattccta ccatcctaaa actaaataaa
    10981 atgatatcgg aaacataaat cttaattcta aactttaatc agattctatt cgaaatttag
    11041 taaaaaaata tttattaaat aagccttctt ctcttttaat ttatgcctag taaaaatcat
    11101 ttttttggca aagatatagt ttcaattaga gatgtatcta gggaggattt agaatttatc
    11161 tttgattcaa ctgataaaat tcagcaatta aaacctgaag aaaaaagtga actaggtaga
    11221 ggaagaacac tcggttatat tttctatgaa ccaagcacta gaactagaat gagctttgaa
    11281 gcagcaatgg catctcttgg gggaagttca ataggaattt ttgattcaaa attttcctct
    11341 atagaaaagg gtgaatctct tgctgacact atcagaataa tggatttgta ttctgatgtg
    11401 atagtcctta gacatgcaag ggatggctcc agtagatatg cagccgaact ttcgtctaat
    11461 cccattatta atgcaggaag tggaagtgaa gagcatccta cacaagcaat gcttgatctc
    11521 tataccattt ttaaagaaaa aggtaggata gataatttga caattggaat agttggtgac
    11581 ctaaaatatg gcagaactgt atattcatta ttgtatggat taaaaaatta tcatgtaaat
    11641 gtacatttaa tttcaccccc cagcttggca attagaaaag aatctatttt tgaatttgaa
    11701 gggataatga acattaagca ttacgatgct cttggtgata taattcccaa actggatgta
    11761 ctgtatgtta ccagaataca aaaagaaaga tttcctgata ctcaagaata cgaaaaagta
    11821 aaaggcactt atagaataga tgaaaaaata ttggataatg gtaaaactga cctgtcaata
    11881 atgcatcctt taccaagact agatgaaatt tctcatacta tagattatac gaagaatgca
    11941 atttatttta aacaagcagc ttatggaaag gagttacgtt cggctttatt ggctttaata
    12001 ttgaatgaag atccatttta atcatttgct aatctattaa ggatttttaa aattcattaa
    12061 actatacctt caaattataa cataaattaa attgtttcta ctaaaagtgg tatagaagat
    12121 ctgggttatc ctgatataca actactgaat ccacattcga tacacatatt acaaccttca
    12181 gtaataatga gtttggagtt acatgatgga caaatctcgt cctcaaagat ttctggtaga
    12241 ggtttttgaa tgaattctat atgttccgtt tctttagttg attctcgggt aggtatgggt
    12301 atgtcgtcta caacgtaatc atcagttact tcaatatcct ttgtatcaaa tagtttagaa
    12361 acatattgag tgacataagg ttcttttatt tgtgattgaa tatattcctt gacatattta
    12421 ctaggtttta caataaacga tttttctaat gtattagttc ctgttatgtg taaaacttgt
    12481 tcatttcttg agccatctcg atatacagtg acacccttta atcccaactc atgcgcaagt
    12541 agataagatg atttgacgtc ctcaacggtt acatcacctg gcatattaat agtctttgct
    12601 atagcattac ttatccactt ctgccataca gattgagcca tgatatgatc ggtccaatgt
    12661 atatcaattg ctgttgtaaa caaggactgc atccatggtg gtatctcatt taatcctctg
    12721 actgatccgt aattattagc tattttagtt aaaatatcat cattatatag accattttcc
    12781 tttaatatat tttcaaatat tttgttagta tagaaaaatc tcccaacagt aacacgtttt
    12841 tcaaaaacta atgcaaagac tggttctaca ccattagaac aatctgctat catcgagaga
    12901 gttcctgtag gtgctatagt ggtcgtccaa gaattacgaa ttccgtattt ctgaattttt
    12961 tcaattaaag aatcccaatc gtaagaatgt ttttgttttg gtatttcata atatccagct
    13021 acaggaattt tttctaatac atactcactt tttttgaata acggaaaaga acctcttgat
    13081 ttagctattg ctacactttc ttccatactg taataagtta ttgtttcagc cagtttgttc
    13141 atgaactcgt atccctcttg tgaagcatat ggtatccgta atgaaaataa tagatcagct
    13201 attcccatga ttcctaatcc tattcgtctt gtaagtttcg tattatagtc tatttcatca
    13261 acaggatatt tattggcatc tataatatta tctaaaaatc ttgatgagat tcttatagct
    13321 tgttcatacc taggccaatc aaactcatac agtccatctg cctttcgttt tacaaaattc
    13381 gataaattaa tggatccaag attacaagac tcatatggat ataatgattg ttctccacaa
    13441 gggttagtgg ctctgagagg tccttctttt gctttaatac atggattgta tttatttatg
    13501 ttatcgaaga atataatacc aggttctgca cttttccatg cacttaatga tataagatca
    13561 attagttgat gggaatctat ctgtttaaga ggttcatggg ttctgggatt tctaagagta
    13621 tatttattat tgttattaat aagagccttc caaaaatcat cccataatcc aacactgaca
    13681 ttaaaatttt caaaaacacc aggttttgtt tttgcagtaa tgaatttttc aatatcagga
    13741 tgccagactt caagaattcc catatttgct ccacgtcttt tgcctccttg tttaacgaca
    13801 tctgtaatcg tatctataat acgcataaag gatgcaggac cagaggcgac tcctgaagta
    13861 gaggcaacaa tatcaccttc tggtctcaaa tcggaataat taattcctac accacctcct
    13921 gatttaaaaa tcatggccgc atctgatgat gttttcatga ttttttccat gtcatcaggc
    13981 atatccaaaa caaaacatgc cgagagttgt cctaaacgag cgccagcgtt catcaaagta
    14041 ggagtatttg ggagaaaatc tttggatatc atcaaattat aatattcttt tatccggtct
    14101 tcataagagg taaactttcc ttgaaaaatc aattttaaaa tatctttaaa cgacgttttc
    14161 atttgtccct tagaagctaa ctgagtgtac aatcgaatta atgattcaaa atgatatttg
    14221 tttaagtaat aagatcctat ctttagttta aggtcaaagt cacttatttt a
//
LOCUS       DASVEV010000069         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1380092, whole genome shotgun sequence.
ACCESSION   DASVEV010000069 DASVEV010000000
VERSION     DASVEV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380092"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..153)
                     /locus_tag="VF222_03470"
     CDS             complement(31..153)
                     /locus_tag="VF222_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141518.1"
                     /translation="MHLLLIAGIIPATFAQNNTGMTSEGGNMTETTQDGNMTQG"
     gene            495..659
                     /locus_tag="VF222_03475"
     CDS             495..659
                     /locus_tag="VF222_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141519.1"
                     /translation="MENDKDIKIETDEDVGLDDKIKAGARALGKKIEDPDKDTGSEYE
                     AEKTKERVVD"
     gene            664..954
                     /locus_tag="VF222_03480"
     CDS             664..954
                     /locus_tag="VF222_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141520.1"
                     /translation="MIHTKLFLIIPSAAILATVLTLATFTIPSFGQSEGTNETMQNAG
                     ESANQTSENMQQGANKTGEAIQGNASGMGSKITEGAKNLAENIGEKLQDLAK"
     gene            complement(1044..1283)
                     /locus_tag="VF222_03485"
     CDS             complement(1044..1283)
                     /locus_tag="VF222_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141521.1"
                     /translation="MEQQQSPIKKKNQQNEDEQEQEEVVIDNIIEELDRKRDIARNKK
                     KKALLKDEMLKYRLEEYTLDYLLFIIRELTGKKLY"
     gene            1324..1455
                     /locus_tag="VF222_03490"
     CDS             1324..1455
                     /locus_tag="VF222_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141522.1"
                     /translation="MMIDTLREMEREKERIDLYGYKHLVFSILGYEIINNNNNNNGC"
     gene            1786..2211
                     /locus_tag="VF222_03495"
     CDS             1786..2211
                     /locus_tag="VF222_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141523.1"
                     /translation="MELFSHFNENDILNLFCDCLQKFGYETIPFNNPIDALNYINNSN
                     TCNCSLIITDYKMHQMTGLDFIKKISEKDTTFKLKILLISAFIKSDIMDNDIPNSLRI
                     DKIIEKPIHIEKLIEEVQKINNRNKLDSYIMFFSLMGNK"
     gene            2369..2518
                     /locus_tag="VF222_03500"
     CDS             2369..2518
                     /locus_tag="VF222_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141524.1"
                     /translation="MMIKTSLFIAEPDSDIQYLYSRFTKQYGFSISDMNIVEKGNKWF
                     RDCIF"
BASE COUNT         1120 a          388 c          449 g         1016 t
ORIGIN      
        1 atcttgagtc attcctgtca tattttcgcc ttagccttgg gtcatatttc cgtcttgagt
       61 cgtttctgtc atatttccac cttctgaggt catccctgta ttgttttgag caaatgtggc
      121 tggtattatt cctgcaatta aaagcaaatg caagaatgga gggagctctt attttgtaga
      181 gtatataggg tcaaataata tagaatcatt attcttgctc tatcttttca ttacaaatac
      241 aaatagtaaa tgtcattagt cgggttcacc tttgaacagt atggtgttca tctacgtatt
      301 aagtaaggaa gttggtcggg agtagggtgc tgtatataat acggttctac taattgacat
      361 ttagatttat agaaattaaa atattagtta gatatgattt tgacataatg atttatagtt
      421 ttacttttac taaatttata ggctagtttt ttaaatctaa ctattatata tcataatgca
      481 taacaacaat tacaatggaa aacgataaag atataaaaat tgagacagat gaagacgtag
      541 gattagacga taaaataaag gcaggagcaa gagcattagg taaaaagatt gaagacccag
      601 ataaagatac tggttcagaa tatgaagcag aaaaaacaaa agaaagagta gttgattgat
      661 atattgatac acactaaatt atttttaata ataccatcag cagcaatcct tgcaactgtg
      721 cttactttag caacatttac cattccatca tttggacaat ctgaaggaac caacgaaact
      781 atgcagaatg caggtgaatc ggctaatcaa actagtgaga atatgcaaca aggtgccaat
      841 aaaacaggtg aagccattca aggaaatgct agtggtatgg gttctaagat aaccgaagga
      901 gcaaaaaatc tcgctgaaaa tattggggaa aaactacaag atttagcaaa gtaaatacac
      961 aatttatttt ttttatgaac tggtttcaaa ctttcctttc gtaacatgct ttgatattaa
     1021 cattcactaa ctttattttt attttaatac aatttcttac ctgtcaattc acggattatg
     1081 aaaagaagat aatcaagagt atattcttct agtctatact tcaacatttc atcttttagc
     1141 aaagcttttt tctttttgtt tctagctata tccctttttc tatccagttc ctcgatgata
     1201 ttatctatta ctacttcttc ttgttcttgc tcatcttcat tctgttggtt cttttttttt
     1261 attggtgact gctgctgttc catatttctc caaagtccaa tctattaatt ctatattctt
     1321 ttcatgatga tagatacatt aagagagatg gagagagaga aggaaaggat agacttgtat
     1381 ggatacaaac atttagtttt ttcaatccta ggatatgaga ttataaataa caacaacaac
     1441 aataatgggt gttaaaggat aaatgagaac ctatacaccc tcaaaagtac acatatatat
     1501 ttatgagttc tttgtagtag taaatgatag taaaagcaga taaaataaaa gattattgat
     1561 tttagtcggg aaggaaaaag agaaaaagaa caaaaataat tccaatcaat aatgacaaaa
     1621 gtacaatcat atttgtcttc atagtattat tattatacaa tttattatat aatttaatct
     1681 tttaaccctt ttagttatag agagggagag atgttagaga atagactcag ttgattattg
     1741 gagaaaatac agcttattta atagagatgc cataaatatt aagttatgga gctcttttct
     1801 cattttaatg aaaatgatat ccttaatctt ttttgtgatt gtttacagaa gtttggctat
     1861 gaaactattc catttaacaa tcctattgat gccttaaatt atattaataa tagcaatact
     1921 tgtaattgtt ctctgattat tacagattat aaaatgcatc aaatgacggg tttagacttt
     1981 ataaagaaga taagcgaaaa agatacgact tttaaattaa aaatcttgct gattagtgcc
     2041 tttattaaaa gcgacattat ggacaatgat atcccaaaca gtttgagaat tgataaaata
     2101 attgaaaaac ctatacatat agaaaagtta atagaagaag tacaaaaaat taacaacagg
     2161 aataaactag atagctacat aatgtttttt tctttaatgg gtaataaatg atttaagcga
     2221 ttataaataa ataaattata ttacgaatct tttaaaataa ttattacgga ttttaaattc
     2281 taatatataa tgttgaataa taattagaga atttgaatag tagtggatac taaaaaaaat
     2341 aacaagaata aaaaaataat gataatagat gatgataaag acatcactat ttatagccga
     2401 accagattca gatattcaat atctttattc tcgttttaca aaacagtatg gtttttcaat
     2461 atcagatatg aatatagtag aaaagggtaa caaatggttt agagattgta ttttctaaca
     2521 caggaaacaa caacgaggtc tcctagtgcc taattgcagc atagaataac taaagatata
     2581 ttaattagca aaatataatt tacaattttt ttattaaaat ttatactata taaagaaaag
     2641 caacatatta gaaaatttgc atataacttt atttaagtat attgtctaaa taatattaag
     2701 aggttctgtt tggagttctt tggactgaaa aaccaaacta gaggacatgt gtcttatatg
     2761 agcctcgatg aaccttcctt cgttattcaa acctaaaatc caatgtataa tggattataa
     2821 attatgttgt ctatattatt tacaatatga atggagatga taataataca acaatatatg
     2881 atcctaaaaa tagtaaacca ataataattg taaaaaattc taattctcaa ttatctgact
     2941 cttctccatc atcatcaaca atagatatag cag
//
LOCUS       DASVEV010000070        11519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1185371, whole genome shotgun sequence.
ACCESSION   DASVEV010000070 DASVEV010000000
VERSION     DASVEV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185371"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF222_03505"
     CDS             complement(<1..499)
                     /locus_tag="VF222_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7141525.1"
                     /translation="MVVGIEIGQIIQDFSVIMIIASAMAFISFKLKQPLVIGYIIAGI
                     IIGPHTPPFSLILNQDVLNLFAEMGIILLLFVVGMEFPIEKLRRIGKKALIIASSEAF
                     GTFAIGFSVSFFLLHFSLFDSAFAALAISVTSTVIIMKVLEELDIIKEEASYIILGVA
                     IMEDII"
     gene            complement(1454..2731)
                     /locus_tag="VF222_03510"
     CDS             complement(1454..2731)
                     /locus_tag="VF222_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7141526.1"
                     /translation="MDILLFFSVLSILSLPESTSSEFIVQDFAVIMVIAAIMLFLTHR
                     FKQSMVIGYLIAGMIIGPFTPPFTLIHQIETVNIFAELGIIMLLFVIGIEFPIAKLKQ
                     IGKISIIVGLTESIGTMIITFFVAQRLGFTTFDSMFLGLAMSITSTVVTIKVLEELGK
                     IKERSSILILGVLIVEDIVAVSVLAILQSIAGAADTEEIAIIPISISIAIAVVFIGSI
                     LILGSKFLPNLIDKIGKTNDYALLLIVILGLAFGLSFIANILGLSVVIGAFLAGVLVA
                     ESRSATVAKVITIPLRDVFSALFFVSIGALVNVSLIPLYIVPVIVLILTAFAVKFLLV
                     LVVLAKSKFDYTDILKVGLGLATAKGEMSLVIAKGGQDVGAITSSILPILSVVTIITS
                     FATPYILKFANNIKISRSNNKITKIDDDDKNNL"
     gene            complement(2859..4415)
                     /locus_tag="VF222_03515"
     CDS             complement(2859..4415)
                     /locus_tag="VF222_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX7141527.1"
                     /translation="MTNKNTSKIKLIKIIIKLLPIIINFKRDRKEWVKREGKNIDENK
                     YRKHAQQALKIFIQLGPSYIKLGQWLSSRSDILPQPYLEVFATLQDDVPVEPFEKVQK
                     IIEEELGNLEEKFDYFDKEAFSGASLGQVYHARYNGKEVVVKISRPGIERIVEKDISI
                     IKKLIPLSTRFIDPNLRFSVEGMFSQFTETIYEEMDYIKEAENLLAIKKNLANEKKII
                     IPNVILDRTSKHIITTEYEPGIKITDVETLDKKGIDRQKLVILIHHIFFKMLLRNNIF
                     HSDPHPGNIAVKDDGSIILYDFGMVGRLDNETRLLLIRLYLGLIDKDPVRTVDVLYQL
                     GTLDYTTNRNIVEKAIELSIRSMHGKHVDKMEVKSLMDLSNKTLSKFPFRLPKNLALY
                     MRMTSIVEGIYHQHKIRFQFVKVLSNLFEQEGLIKDAYIEEIKYSFQKFVKNIEASVN
                     LSQTIKTYLDNQETNIKKTDKLYTFLGKSIFASALFVGSASLFSYNPIISSIGFISSI
                     ILFVFSFIKK"
     gene            complement(4450..4875)
                     /locus_tag="VF222_03520"
     CDS             complement(4450..4875)
                     /locus_tag="VF222_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141528.1"
                     /translation="MNYIDGDGNILIPKSVRPIIDYTETNLGSKKGAKKQFRYENLHI
                     REYENYYSVHMDRVDPRIDPLGHLLKDAPEYITCWIIASTIGLKVGTTFYKKRKEKDN
                     GSQLKALPLGIITGIFAGTTTYMTTYHFHNFIRKAKLRK"
     gene            5014..5550
                     /locus_tag="VF222_03525"
     CDS             5014..5550
                     /locus_tag="VF222_03525"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7141529.1"
                     /translation="MNKVKLLHSKRVYAGRISIRKDEFILQDKKIQKEIVEHSPSVGL
                     VPIIDYSNILLITQYRHPAKRTLLEIPAGKIEDGETKEQAARRELYEETGYAGTLSFL
                     TKWYLAPSYDTELMYIFLVTNLKKMSDKQGRDADENIKLKKLSLKTALKKCINGEIID
                     CKTVAALLVYQEYVKQMV"
     gene            complement(5640..5978)
                     /locus_tag="VF222_03530"
     CDS             complement(5640..5978)
                     /locus_tag="VF222_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HEX7141530.1"
                     /translation="MMRGGNREMRRMLGKMGLDMNEMEGIEEVIIKTETKEMYLIKPQ
                     VVEMKGKDNTIFQIIATDIEEKQRETPSLKEEDIILVMQQANVSKEKAIQALTDSKGD
                     IAQAILTLTT"
     gene            complement(5995..6474)
                     /locus_tag="VF222_03535"
     CDS             complement(5995..6474)
                     /locus_tag="VF222_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEX7141531.1"
                     /translation="MKPLPLNPRNKLINHISVLFDTNVSEEFPEIQCEFSKRTGRIKN
                     FSIESDLFATLRKDGGLALTIFGAKMMVKYTQFVENCIIAEDDVIPFISEGRSLFCKH
                     VKWCGKNVNSGSDVVVIDSRYEVLAVGKAVCGNKIMKKFDYGVAVKIREGIKSRNNQ"
     gene            6767..7231
                     /locus_tag="VF222_03540"
     CDS             6767..7231
                     /locus_tag="VF222_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HEX7141532.1"
                     /translation="MVSFCELCGNSIQSPKNVIIDGSIFNVCLSCSKRGKPYEPNQPS
                     KKTFPPKNASIPMSQKKPIKVYHPKAIPITDEKILRPDFGKIIREARMKKGITQENVA
                     NQLSEKITLYKKIETGGIKPNEILSKKLERCLGIQLYENLVENNENEDDFSK"
     gene            7228..8334
                     /gene="hflX"
                     /locus_tag="VF222_03545"
     CDS             7228..8334
                     /gene="hflX"
                     /locus_tag="VF222_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7141533.1"
                     /translation="MNFKNALLITYPIEESVNEALSLAETAGYVVKKIVKQKHITQSK
                     YGIGKGKAYEIKDLVNDLNIDIILFDEVLKPTQQYNLAILCKRDIIDRERLILEIFER
                     RASTSESRIQIKLAQLRYEMVRIKEKVKLAKMGEQPGFFGLGKYDADVYSLDLKSQMS
                     VLKKKLEKEEKRRTLYRNQRNRMGISTISLVGYTSSGKTTLFNSLTGESKEVGKEMFT
                     TLSTFTRSLYKNSNQKLLLSDTVGFVNKLPAYMIDAFKSTLHELVFSKLILLVIDVSQ
                     TIKNIRINLNSCLHIINELQIPSTKILYLLNKVDLTTLNNAYEKAKMLDLKYPNNIVL
                     PISAKTGYNVNKILDLIDMLVYGNNLEIQNSGDL"
     gene            8337..8873
                     /locus_tag="VF222_03550"
     CDS             8337..8873
                     /locus_tag="VF222_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HEX7141534.1"
                     /translation="MNIAVLRIGHRLVRDDRTTTHVALVSRSFGCNILYMTDVNDDIK
                     STIDDVNNRWGHASNFKIEIVKGWRNVILNWKKNGGIVIHLTMYGLNVDDIIGKIKNS
                     SNDILIIVGAEKVPKEIYKLSDYNIAIGNQPHSEISSLAIVLDRIFQGKQLKLNFESA
                     KMKIIPSLREKLVEKNYF"
     gene            9023..10855
                     /locus_tag="VF222_03555"
     CDS             9023..10855
                     /locus_tag="VF222_03555"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX7141535.1"
                     /translation="MKKNEELTKINHEKGSFVKPKEKWGLEKETDNFANKVKLYRQGK
                     LSDDDFRRFRLQHGAYGSRLRPDYSMIRIKVPSGEITPEQLEKIANLSEAFSIGSAHV
                     STRQNIQLHWVQLEDVSEVMRGLVEVGLTTREACGNTVRNVMCSHFAGVCPNEVFDAT
                     PYSTAIAKFLLRNPMSQNLPRKFKINFGCCNKHGLVRIADIGLVPDLRDGKKGFRIYL
                     GGGLGAASFIGHLLEDFTPEEQLLSTCMATIRLFDRLGNRDNMARNRMRYLVNETGWE
                     KFQKMVLKERTIVEMTVSEFTKNLYQVHIDSYVPIKKSTKFNKLPIVETNISTSSNSY
                     DRWLHTNVVPQKQEGYFTIFITLGAGDITANQLRSLASLIRKFSSESIARNTPQQNFA
                     IRYIYNNDLPAIYKKLMAIGLANPGALTITSAVGCSGTTSCNLAITNSHRLAKEVQRT
                     LLELGFDVDNDLSNSTIKISGCPNSCGQHEIATIGFFGGAARIENSMAPVYTMLFGGN
                     GYEDGKLGKAIMRVPAKRVIDVIQKIVELYKNEKSSSETLDMWIDRIINSNGSGNIKN
                     IDDIKTLLIPVTQLPSFEEGQEFYQDYGNDIKFVAKTARGECAA"
     gene            10852..>11519
                     /locus_tag="VF222_03560"
     CDS             10852..>11519
                     /locus_tag="VF222_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7141536.1"
                     /translation="MINLKNNKVQGELKYDIQSKSKNVNVKSSILKQQNQKKEINDTK
                     TQFKVKGTIYKLPIVCDVETLRTLIRKKAVRVVDVRRIEDYKKGHIPTAVSLPLANLL
                     SNDSPEQIVKLLENLGIYDEMPVVVYDDTFGALAARVAWTFQYVGHDNTSLLEMTYTN
                     WKNLGLEIENRINKFPETTHSLKLNQSIYADATYVESIQNNPDKVLVDSRERLNFLSE
                     HIPSA"
BASE COUNT         4221 a         1652 c         1632 g         4014 t
ORIGIN      
        1 ttataatatc ttccattatt gctactccta aaattatata ggatgcttct tctttgataa
       61 tgtcaagttc ttccaatact ttcataataa taacagtact ggtaactgaa attgcaagtg
      121 cagcaaatgc tgaatcaaaa agtgagaaat gtagtaaaaa gaaacttaca gaaaaaccta
      181 tggcgaatgt tccaaatgct tctgatgatg caattatcaa tgctttcttt cctattcttc
      241 gaagtttttc tataggaaat tccataccaa caacaaataa caaaagaata atacccattt
      301 ctgcaaataa gttcaagacg tcttgattta atattagact aaatggagga gtatgaggac
      361 ctattattat acctgcaatt atatacccaa taaccaatgg ttgttttaat ttgaacgaga
      421 taaaagccat tgcagaggca atgatcatta tgactgaaaa atcttggata atttgtccta
      481 tttctatccc cacgaccatt tttattaaat aataataaaa ataaagagta taataactaa
      541 aggaaatatt acaaataaca tgttattaga aagaataata tcataataac aatttcaatt
      601 aatctactcc gtctgactgt ttattctcaa tataatctct tttttacata tcttcttttt
      661 catttattgt aatgaaacaa ttctacatct ttcacattgg tatccatgat gatatactaa
      721 tatataaaaa aataatataa aaatattcaa ataaatgaat cagttacaat ggttctttat
      781 ttattcgaat tatattaaaa aaagtaactt aaattataat tgagtcatca tataataaat
      841 aaacgataaa aaattaaatt ttctttcttt ttgattaata ataatattgt tgtttttgtt
      901 agtttattat caaaatattt aattaaatac tctctaatca tcaataatct tttaataaat
      961 tgatagagat tgtttgctct atagattatc taatattttg ctttattatc attttcttcg
     1021 tcctcgaaat ctttttttta tctatagctt ctttatatct tgataaaggt tttggaaatt
     1081 ttacttttaa cgtaaatttt ctaggagtta aatttatatc gctatactcg agaaaagttt
     1141 tacagttatt tattttatcc ctaaatgtta caagagaaat tataattatt ctgtaaaaat
     1201 acacagatat catttaaaat atcaaatgta taattagtaa cataatagta taatgaaaat
     1261 aaaataaagg tatcttttaa tatattttac aaaatcccac ataaaacata tgaaatatta
     1321 taatcattac acaagtaaca aaatattatt atttgtaata actagtaatg aaatatttat
     1381 gttttttaac ataatatcat aaacttaagg taactaattg tcaaaagtgt ctattaaagg
     1441 tcttgtacat ctatcaaagg ttatttttat catcatcatc aattttagtg attttattgt
     1501 ttgatctaga aatttttatg ttatttgcaa attttagtat atatggagta gcaaaagatg
     1561 taattattgt aacaacactt agtattggta atatagatga agtaattgca cccacatctt
     1621 gtccaccctt tgctatgact aaagacattt ctccttttgc tgttgccaat cctaaaccta
     1681 cttttaatat gtcggtatag tcaaatttgg attttgctaa tacaactaat actaaaagga
     1741 attttacagc aaaagctgtt agtatcaata caattacagg aactatgtat aatgggataa
     1801 gtgaaacatt cactaatgct cctatagata caaagaataa tgcagaaaat acatccctta
     1861 atggtatagt aataactttc gcgactgttg cacttctaga ttcagccact aaaactccgg
     1921 ctaaaaatgc acctattaca acggataaac ctaaaatatt cgcgatgaag gatagaccga
     1981 aagcaagtcc taatattact attaagagaa gagcataatc attcgttttt cctattttgt
     2041 ctattaaatt aggtaaaaat tttgatccaa gtataagtat acttccaata aaaactactg
     2101 ctatagctat cgatattgat ataggaataa ttgcaatctc ttcagtatct gccgcacctg
     2161 ctatggattg taatatggct aaaactgata cagccacaat atcttcaact atcaaaactc
     2221 ctagtattaa tattgaggaa cgttctttta tttttcctaa ttcttccaat accttgatag
     2281 ttactaccgt actagtaata gacatggcta atcctagaaa cattgagtca aatgtagtaa
     2341 atcctaatct ttgggcaaca aaaaaagtta taatcatagt tccaattgac tctgttaatc
     2401 caactattat tgaaatttta cctatttgtt tgagttttgc tataggaaat tctataccaa
     2461 ttacaaagag aagcattatt atacctaatt cagcaaatat gtttacagtt tctatctgat
     2521 gaattaaagt aaaaggtgga gtaaacgggc ctataatcat tcctgctatt aagtaaccaa
     2581 tgaccatgga ttgtttaaac ctatgagtta aaaataacat tatagcagca ataaccatta
     2641 taacagcaaa atcttgaaca ataaattctg aagatgttga ttcgggaagg gataaaatac
     2701 tcaatactga aaagaacaat agaatatcca tggcccattc attatataat catctaaaat
     2761 aaaaatcaag aggatttata aagaaaatcc tgtgaagtta tttctttaat cgctctttta
     2821 ataataaata ttcaaaaatt tattattgat atatgatttt atttttttat aaaactaaaa
     2881 acaaataata ttattgaaga aataaaacct atagatgaaa ttataggatt gtatgaaaat
     2941 aaagatgcag atcctacaaa taatgcagat gcaaaaatac tcttacctaa aaatgtataa
     3001 agcttatctg ttttcttgat atttgtttct tgattatcta aatatgtttt tatagtttga
     3061 gataaattta ccgatgcttc tatatttttt acaaatttct gaaaagaata ttttatctct
     3121 tcaatatatg catccttgat tagtccttct tgttcgaata aattcgatag aacttttaca
     3181 aattggaatc ttattttatg ttgatgataa atcccttcca caattgaggt catcctcata
     3241 tataatgcta aatttttggg taatctaaat ggaaatttac ttaaggtctt atttgataaa
     3301 tccattaaag atttcacctc cattttatct acatgttttc catgcataga tctaatactt
     3361 agttcaatag ctttctcaac aatatttcta ttagtggtat aatctaatgt tccaagttga
     3421 tataatacat cgacagttct tacaggatct ttatcaatca aacccaaata tagtctaatc
     3481 aacaataatc tagtttcatt atcaagtctt ccaaccattc caaaatcgta tagaattatt
     3541 gaaccatcat ctttgacagc tatatttcca ggatgaggat cagaatgaaa aatattatta
     3601 cgcaatagca tcttgaaaaa tatatggtgt attaatatta ctaatttttg tctatcaata
     3661 ccttttttat ctaatgtttc tacatctgtt atctttattc ctggttcata ttctgtagtt
     3721 ataatatgtt tagaagttct atcaagtatt acattaggga ttataatttt tttctcatta
     3781 gctagatttt ttttaattgc taatagattc tctgcttctt ttatgtaatc catttcttca
     3841 taaatagttt cagtaaattg agaaaacatt ccttcaactg aaaatcttaa attaggatca
     3901 ataaatctag tagataaggg aatcaatttt tttattattg aaatatcttt ttctacaatt
     3961 ctttcaattc ctggtctgga gatctttact actacctctt ttccattata tcttgcatga
     4021 tatacttgtc ctaaactagc gccagaaaaa gcctctttat caaaataatc aaacttttct
     4081 tctaaatttc ctagctcctc ctcgattatt ttttgtactt tttcaaaagg ttctactgga
     4141 acatcgtctt gtaaagtagc aaatacttct aaatatggtt gcggtaatat atcagaacga
     4201 gaagataacc attgacctaa ttttatataa gatggtccca gttgaataaa aatttttaag
     4261 gcctgttgtg catgttttct gtatttattt tcatcaatat tttttccttc acgttttacc
     4321 cattcttttc tgtctctctt aaaattgatt attattggta gtagctttat tatgattttt
     4381 attaatttaa tttttgaggt attcttgttt gtcatttcgg aaagaatatt taaacaataa
     4441 tcaaaatttc tattttctta attttgcttt tctaataaaa ttatggaaat gatatgtagt
     4501 catatatgtt gtagttccgg caaaaatacc tgtaataatt cctaatggca atgcttttaa
     4561 ttgactacca ttatcttttt cttttctttt tttataaaat gttgtaccta cttttagacc
     4621 aatagtacta gctataatcc agcatgtaat gtattctgga gcatctttta agagatgtcc
     4681 aaggggatcg attcttggat cgactctatc catatgtacg ctataataat tctcatattc
     4741 tctgatatgc aaattctcat atctaaattg ttttttcgct ccttttttac ttcctagatt
     4801 tgtttcagta taatcaatta taggcctgac ggatttagga ataaggatat tgccatcacc
     4861 gtcaatatag ttcatctata ttagaattaa acaaatatca tataaaacta acttaatatt
     4921 tatttattga atatagtaag ataaaactct gataatgtag gaaagtatat tttactttgg
     4981 ataaaaaata ttctcaattt atagttgaat caaatgaata aagttaaatt attacatagt
     5041 aagagagtct atgcaggaag gatatcaatt agaaaagatg aatttatttt acaagacaaa
     5101 aaaattcaga aagagattgt tgaacattct ccatcagtag gacttgttcc tataatcgat
     5161 tattcaaata ttcttctaat cactcagtat agacatccag caaaaaggac tctattagaa
     5221 atacctgctg gaaaaataga agacggagaa accaaagagc aagctgctag aagagaattg
     5281 tatgaagaga caggttacgc aggtacatta tcttttttga ctaaatggta tttagcacca
     5341 agctacgata cggaactaat gtacattttt ttagtaacaa atttaaaaaa aatgagcgat
     5401 aaacaaggta gggatgctga tgaaaatata aaattaaaaa aactaagcct aaaaactgca
     5461 ttaaaaaagt gcatcaatgg tgaaattata gattgtaaaa cggttgcagc attacttgtt
     5521 tatcaagaat atgtcaaaca aatggtataa aaataataac tataaaatgg tataatattg
     5581 caaattcaaa taattctttg atataattaa tgaataaaat aaaatataat tatttataat
     5641 catgtagtta gagttaagat agcttgtgct atgtcacctt tagaatcagt caaggcttga
     5701 atagcttttt cttttgatac attagcttgt tgcattacta agattatgtc ctcttctttt
     5761 aacgatggag tctctctttg tttttcttct atgtctgtag caataatttg aaaaattgta
     5821 ttatcttttc ctttcatttc aacgacttgc ggttttatta gatacatttc tttagtctca
     5881 gtttttatta ttacctcctc aattccttcc atctcattca tatctagacc catttttcca
     5941 agcattcttc tcatttctct atttccccca cgcatcatta tctattacct aagatcattg
     6001 attgtttcta ctttttattc cttctctaat ttttactgct acaccgtaat caaatttttt
     6061 cattatcttg tttccacaaa ccgcttttcc tacggctaac acttcatatc tactatcaat
     6121 gactacaacg tctgatccac tattaacatt ttttccacac cactttacat gtttacaaaa
     6181 taatgatctt ccttcactaa tgaatggtat tacgtcatct tctgcaatga tacaattctc
     6241 tacgaattgt gtatatttta ccatcatttt tgctccaaat atagttaaag caagtcctcc
     6301 atctttacgt aaagtagcaa ataaatcgga ttctatagaa aaatttttaa ttctgcccgt
     6361 tctctttgaa aattcacatt gtatttctgg gaactcttca cttacatttg tatcaaaaag
     6421 gacagatata tgattaatca gtttatttcg ggggtttaaa ggtaaaggtt tcaatattcc
     6481 agatatctaa gaaaatgttt cttatatgaa aatatttgta tttctttatt gtattactct
     6541 atatactaga aaattaaatt ttaaataaca aaatgccttt tctaactaat tctaaaaata
     6601 tgataaatat aattacacac aggaatactg ctcttatttt ttactttgga acattaattc
     6661 agcgttagag atattataaa caaaatactt tagaaaactt atataaccgt aaacaccacg
     6721 tgttaaaaaa gaaagacttt atttcttgtt tagatattgt tatattatgg tctcattctg
     6781 tgaattatgt ggaaattcca ttcaaagtcc aaaaaatgta attattgatg gtagtatttt
     6841 caacgtatgt ttatcgtgtt caaaacgtgg aaaaccatat gaacctaatc agccttcaaa
     6901 aaaaacattt cctccaaaaa atgcatccat tcccatgtcc caaaaaaaac caattaaagt
     6961 ttatcatcca aaggcaattc caattactga tgaaaaaata ttaagaccag attttgggaa
     7021 gatcataaga gaagctagaa tgaaaaaagg aataactcaa gaaaatgtag ctaatcaatt
     7081 aagcgaaaaa attactttat acaaaaaaat tgaaacaggc ggaattaaac caaatgaaat
     7141 attgtctaaa aaactagaac gttgtctggg aattcaatta tatgaaaatc ttgttgaaaa
     7201 taatgaaaat gaagatgatt ttagtaaatg aattttaaaa atgctcttct aataacatat
     7261 cctattgaag aatctgttaa tgaagcctta tctttggctg agaccgcagg ttatgtagtt
     7321 aaaaaaatag tcaaacagaa acatattaca caatcaaaat atggaattgg taaaggaaaa
     7381 gcctatgaaa ttaaggatct ggtaaatgat ctgaatattg acataattct ttttgatgag
     7441 gtattaaaac ctacacaaca atataattta gcaattttat gtaaaagaga tattatcgat
     7501 agagaacgat taatattaga aatattcgaa cgtcgggcca gtacatcaga atcacgtatc
     7561 caaattaaat tggctcaatt aagatatgaa atggttagaa taaaagaaaa ggtaaaactt
     7621 gcaaaaatgg gtgaacaacc aggatttttt ggtcttggga aatacgatgc tgatgtttat
     7681 tcattggatc ttaaatcaca aatgagcgtt ttaaagaaaa aactagagaa agaggaaaaa
     7741 agaagaacac tatatagaaa tcaacgtaac agaatgggta tttcaacaat ttcattggtt
     7801 ggttatacgt catcaggtaa aactacactg tttaattctt taacgggaga atcaaaagaa
     7861 gtaggcaaag aaatgtttac tactctatct acgtttacta gatctttata taaaaattcc
     7921 aatcaaaagt tattattatc tgacactgta ggatttgtaa acaagttacc agcttatatg
     7981 atcgatgctt ttaaatcaac tctacatgaa cttgtttttt ctaaattgat tttgttggtt
     8041 atagatgtta gtcaaactat caaaaatata agaataaact tgaatagctg tcttcatata
     8101 ataaatgaat tacaaattcc atcaacaaaa atattgtatt tactaaataa agttgattta
     8161 actaccctaa ataatgctta tgagaaagct aagatgttag atttaaaata tccaaataat
     8221 atcgttttac ctatatctgc aaaaactggc tataatgtaa ataaaatatt ggatctaata
     8281 gatatgctcg tttatggaaa caatttagaa atacaaaata gtggagattt ataaaaatga
     8341 atatagcagt gcttcgaata ggtcatagat tagtcagaga tgatcgtaca actactcatg
     8401 tagccttagt ttctagatct tttggttgta atattctata catgactgat gttaatgatg
     8461 atataaaatc tacaatcgat gatgtaaata ataggtgggg tcatgcatca aattttaaaa
     8521 ttgaaatagt caaaggttgg cgaaatgtaa tattaaattg gaagaaaaat ggtggtatag
     8581 ttattcatct tacaatgtat ggattaaatg tagatgatat cattggtaaa attaaaaata
     8641 gtagtaatga tattttaata atagttggag ctgaaaaagt tccaaaggaa atatacaaac
     8701 tttctgatta taatattgct ataggtaatc aaccacattc tgaaatttct tccctagcta
     8761 ttgttttaga tagaatattc caaggaaaac aacttaagtt aaattttgaa tcagccaaga
     8821 tgaaaattat tccttcactt agagaaaaac tagtagaaaa aaattatttc tgaaaggagg
     8881 aattataatt ttactcatcg tcaatagccg tttaataaac tataatattc atacaataac
     8941 ttgccatcta ctatgaaatc agttaatttt tttagttgtg attccataaa aaatataagg
     9001 ttttgtataa ccaattagta tattgaaaaa gaatgaagaa ttaactaaaa ttaaccatga
     9061 aaaaggttct tttgtaaaac ctaaagaaaa atgggggtta gaaaaagaaa ctgataattt
     9121 tgctaataaa gttaaactct atcgccaagg aaagttatct gatgatgatt ttcgaagatt
     9181 taggcttcag catggagcat atggatcaag attaagacct gattatagta tgataaggat
     9241 taaagttcct agtggcgaaa tcactccaga gcaactagaa aaaatagcta atttatctga
     9301 ggcattttct attggatcag ctcatgtttc aactagacaa aatatccaat tacactgggt
     9361 acagttagaa gatgtcagcg aagttatgag aggattagta gaggtaggtt taacaactag
     9421 agaggcttgt ggtaatactg taagaaatgt aatgtgtagc cacttcgcag gtgtttgtcc
     9481 taacgaagtt tttgatgcta ctccttattc aacagcaatt gcaaagttcc tattacgcaa
     9541 tccaatgtct cagaatttac caagaaaatt caaaattaat tttggatgct gtaataaaca
     9601 cggtctggta aggatagctg acattggttt agtacctgat ttaagagatg gaaaaaaggg
     9661 atttagaatt tatcttggag gcggattggg tgctgcctcg tttataggtc atcttttaga
     9721 agatttcact cctgaagaac aacttttatc aacttgtatg gcaactatta gactatttga
     9781 tagactagga aatagagata atatggccag aaatagaatg cgctatttag taaatgaaac
     9841 tggttgggag aaatttcaaa aaatggtatt gaaggaacgt actattgtag aaatgactgt
     9901 ttctgaattt actaagaacc tttatcaagt tcacattgat tcatatgttc caataaagaa
     9961 gtcaacaaag ttcaataaac tccctattgt agaaacaaat atttctacta gtagtaatag
    10021 ttatgatcga tggttgcata cgaatgtagt tccacaaaaa caagaaggct attttacaat
    10081 atttatcaca cttggtgcag gcgatataac agctaatcaa ctaagatctc tagcatcgct
    10141 aatacgtaaa ttctcatccg agtctattgc aagaaacacc ccacaacaaa attttgcaat
    10201 tagatatatt tacaacaatg atttgccagc aatctataaa aaacttatgg ctattggatt
    10261 agctaatcct ggtgccctta caatcacttc cgcagtaggc tgttcaggta ctacttcttg
    10321 taatctggct ataactaatt cacatagatt agctaaagag gttcaaagga cattgttgga
    10381 attaggtttt gatgttgata acgatcttag taattcaact attaaaataa gtggttgtcc
    10441 taattcttgt ggacaacacg aaattgcaac tattggcttt tttggtggtg ctgcaagaat
    10501 agaaaattct atggctcctg tgtatactat gttatttggg ggtaatggtt atgaagatgg
    10561 aaaactaggt aaggctatta tgcgtgttcc agcaaaaagg gtaatagatg taattcaaaa
    10621 aatagtagaa ctatacaaaa atgaaaaatc ttcttcagaa acattagaca tgtggataga
    10681 ccgcattata aatagtaatg gaagtggtaa cataaagaat atagatgata taaaaactct
    10741 tcttatacca gtgactcaac ttccttcatt tgaagaaggt caagaattct atcaagatta
    10801 tggtaatgat attaaatttg tcgcaaagac tgctagaggc gaatgtgcag cttgattaat
    10861 ttaaagaata ataaagttca gggagaactt aagtatgata ttcaatcaaa gtctaaaaat
    10921 gtaaatgtaa aaagctcgat tttaaagcag caaaaccaaa agaaggaaat taacgataca
    10981 aaaacacaat ttaaagtgaa aggaactatc tacaagcttc ctattgtatg tgatgtagaa
    11041 acgttacgta cattgattcg aaaaaaagca gtacgagttg ttgacgttcg tagaatagaa
    11101 gattataaaa aaggacatat acctactgca gtatcattac ctctagcaaa cttactttca
    11161 aatgactcac ctgaacagat tgtcaaattg ttagaaaatc ttggtatcta tgatgaaatg
    11221 cctgtagtgg tatatgatga tacttttgga gctttggcag cacgagttgc ttggacattt
    11281 caatatgttg gtcatgataa cacttcactt ctggaaatga cctatacaaa ttggaaaaat
    11341 cttggtttgg aaattgaaaa tcgcattaat aagttcccag aaacaaccca ttctcttaaa
    11401 ttaaatcaat ctatttatgc agatgctaca tatgttgaat ctatacaaaa taatcctgat
    11461 aaggtattag ttgattctag agaacgactt aattttttat ctgaacatat tcctagcgc
//
LOCUS       DASVEV010000071         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_215437, whole genome shotgun sequence.
ACCESSION   DASVEV010000071 DASVEV010000000
VERSION     DASVEV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215437"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VF222_03565"
     CDS             <1..655
                     /locus_tag="VF222_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7141537.1"
                     /translation="IKEPILVMIVLAGASALSLHRAFQMRKIANKYNLLSLKTDARNS
                     IKDGSASVIGFFSILIATQFGFLQMDAIGGMIIAGYIFSVSYISLKRSSLILVDSWQN
                     PDLTDMIKKIISEEFSKEAVGVKKVLLRSSGMVDQAEIHIELSGKESLSDIEMLSLGM
                     EMAIRAKIPSLEKIAIIPHSAASSTIADKSASTKMNALRYIKWRQSAFRFNRNRQTK"
     gene            1327..1584
                     /locus_tag="VF222_03570"
     CDS             1327..1584
                     /locus_tag="VF222_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141538.1"
                     /translation="MPRDSTIYNPEDIIKKEARGLGDDADFGEVQEVGVEYIVTQKGI
                     LDRDKYYIPKNLVDRFEDGIVYLRVTKEEAKQYKEHETNLI"
     gene            2112..2585
                     /locus_tag="VF222_03575"
     CDS             2112..2585
                     /locus_tag="VF222_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141539.1"
                     /translation="MSAQGKVYDEYSNISTNLTQIFGEPIYKETSRQESDSIVLDVKE
                     SIKTQDSYIATGILKDVGNVTDKSTFVTTHQPDGNISSSKGHGIITTVDGEIATYTGK
                     DLGMTDINGTETYRGIEIFQTESDGKLAFLNNLIGLYEYKSWLDGKKSGIIWEWK"
BASE COUNT         1340 a          520 c          572 g         1324 t
ORIGIN      
        1 gataaaagaa ccaatcttag taatgattgt gttggctggt gctagtgcat tatcattaca
       61 cagagcattt caaatgcgaa agattgccaa taaatacaat cttctttcac ttaaaacaga
      121 tgccagaaat tcaataaagg acgggtcagc atcagtaata ggtttcttta gtatactgat
      181 agcaactcaa tttggatttc tacaaatgga tgcaataggt ggaatgataa ttgcgggata
      241 catattctct gtttcctata tatcattaaa gagatcatct cttatacttg tagattcatg
      301 gcaaaatcct gatttaacag atatgataaa gaagataatt tctgaagagt ttagtaaaga
      361 agctgttggg gttaaaaagg tattgctgag gtcttcaggg atggtcgatc aggcagagat
      421 ccatatagag ttaagtggta aggaatcact gtcagatata gaaatgttat cactaggaat
      481 ggaaatggca attcgtgcga aaattccttc acttgaaaaa atagccatta ttcctcattc
      541 agcggcgtct tctactatag cagacaagtc tgcttcaact aagatgaatg cattacgtta
      601 tattaaatgg agacaatccg catttagatt taatagaaat agacaaacta aataaaacaa
      661 gagataaatg cataacatat aaagcatcat ataatatcca ccttcaaatt ctgtattttc
      721 atgattaatg tttattatat tcgctttctt ctaaaggaag agcacaattt gactagtgca
      781 aagtgactat cgattttatt tggatctact aattgttcct ttcccactaa atatggtatg
      841 gttattttgt tgttgtcgtg atagttgttg tcgtcatcat agtctgaata tatctataat
      901 ctgacttgga gcctatttag gctatgacaa attctagcaa aataaaattg tatcaattat
      961 caaatattat atccatcaaa tatttcatcc ataagggtgt tctaccattt cataatctta
     1021 gtgtatagta gtttgtgtat gcctatgcta ctactataat tctaaggttt ttgctatcta
     1081 caaaataaac aaaattgtct aaaattatca aatattatat agaaacataa tttccagtga
     1141 cctgttaagg aaagacctca agtctgccat agcctcacct actgctatta tttgctccta
     1201 cttatgatag tagtgatcta ttatggcttt aactaaataa ttaaattgta tcaaaccatt
     1261 agagatgata tattacagat atatttgaac taaattagtt atataaaatg tatatcaagt
     1321 tattatatgc cacgtgattc tactatctac aatccagaag atattattaa aaaagaagca
     1381 agaggattag gtgatgatgc agactttggt gaagtacaag aagttggcgt agaatacata
     1441 gtaacacaaa agggtatttt agacagagac aaatactata ttccaaagaa tttggtagac
     1501 cgatttgagg atggtatagt ctatttaaga gtgacaaaag aagaagcaaa acagtacaaa
     1561 gaacatgaga ccaacttgat ataatttttt ttatttatat cgatttttcc ttctaccgta
     1621 tctaaccttt cagataacat aattaattta tcagataaat ttgcaatgaa tcttctttca
     1681 ttttgtcttt ttctcttata gtttggttat gttctcaatt tttggtacta aatttgcacc
     1741 ttatctttcc aagtgctgaa tatatagaaa agtcaaataa ggatcatttt tcttctaata
     1801 gtattacttg attcccaact ttattaaaat taattttttg tctaggattt aacgtatatt
     1861 ctagattcgt acccttacat cttttaacaa tatttttaca ctactttcgt aatatggagg
     1921 atgatgagaa aataaatgaa aaagaacttc gtccgtatga taacgtaatg aattttttat
     1981 caatctgtgt aaatacaata attaatgata taaactagtt ctaccaaata ttaatacaaa
     2041 gttatgaaaa attagttttt ggcattgtct tatttgtgtt ttccatattt ttatcaaatg
     2101 gattgtcaat tgtgagtgca caaggaaaag tttatgatga atattcaaat atatctacta
     2161 atttaaccca aatatttgga gagcctattt acaaggaaac aagtcgtcag gaaagcgatt
     2221 caatagttct ggacgttaaa gagagcatta aaactcaaga ctcttatatt gcaactggaa
     2281 tcttaaaaga tgtaggaaat gttaccgata aatcaacgtt tgtgacaaca catcaacctg
     2341 atggtaacat atcttctagt aaaggtcatg gtattataac gactgttgat ggtgaaattg
     2401 caacgtatac tggaaaggat ctaggaatga ctgatataaa tggaactgag acatatagag
     2461 gtattgagat ttttcaaact gaatcggatg gaaaacttgc attccttaat aatttaatag
     2521 gattatatga atacaaatct tggttagatg gaaagaaatc tggtatcatt tgggaatgga
     2581 aataattatt taaaatacat cattttattc tttatcaagt ttttataatg attttctata
     2641 aatactacat catggtagaa ttatacttca aaacacataa caatcagaat ttcaaattta
     2701 tggagatttt agaatattga gaaagaatac ttggatgcat attataagac aggcgacgaa
     2761 ttaaccaatt tgataatttt gattaactag gttcaccgat ttttaaatat ctgactcatc
     2821 tattaaatac taatctaatc atcacaacaa cacgtcaagg aagcctattt caaaaaaagc
     2881 catagtctgt atatatctgc catggacttc tgcaggctat ggcttctttt acaaataaat
     2941 tatctcgaat tattaaatag atcattaaca aatgactata catctaagat aaaaacttgg
     3001 tttgaccctg gtcctctatt gttgtggttt actccttaga ggcctatttt ttttacataa
     3061 attaatcatt aatctaatat ctttagacta tgatgattag caattgaaca ttgaaattgt
     3121 gttaattcaa atctagatgg accagtaatt tatactaaat tttatatgaa atatacaatt
     3181 gtttaatcct ttaagttcct gtgaaattac tatatttttc tataatataa tattaagttt
     3241 ccatttaatt ttaagtttat acttaatcca aattttaatc tcaaattatt aataatagta
     3301 aattcatatt tttataaatc tttccgtcca ataactattt gataaacaat tgtagagttg
     3361 tcatatttag actttttaat ctcactcaca cagttctggt gttttattat gttaattaat
     3421 taaaacaatt actaaatatt ttaataccta gtagtggaac caactttgat ggacgagggt
     3481 tcgagtccac tctcggcgtt caacaccagt agtcatagag ttaaggtaaa attatagtat
     3541 tagaaacaga gtatattgtt atagaaattt caataaatta tgctaaatgt tgtaaagttt
     3601 atatatccat ataacgatga atatcttgca atcattattt gaatgagtat aggatcgtta
     3661 ttacacggtt gactgtttga atattgaaat gacaaacaca agtatagttc agcactaatt
     3721 taatttgtta ttacacaaat atgtgattag ttgaat
//
LOCUS       DASVEV010000072         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_20631, whole genome shotgun sequence.
ACCESSION   DASVEV010000072 DASVEV010000000
VERSION     DASVEV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20631"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..404
                     /locus_tag="VF222_03580"
     CDS             129..404
                     /locus_tag="VF222_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141540.1"
                     /translation="MGNRSRTEIVGSILDAANGGATKTKIMYIAFLSFNQLKEYLSIL
                     IESNLIEYLDGTKTFKTTEKGLNLLKIHNEMGELLQQSNTINTNDLI"
     gene            919..>2060
                     /locus_tag="VF222_03585"
     CDS             919..>2060
                     /locus_tag="VF222_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7141541.1"
                     /translation="MHKSSIIGFFLVSSLIFGSGNMMILQGAAAQDYYNDDRQYKDSS
                     SSGYRDDNNDNNYYNNNNYYPPKDFDNNNKKYVCKDGPFEGLLTSSVEFCKPDITVPT
                     DFPSIQAAINASNPGDTIKVLPGIYTEQIIISKSLTIIGSGAKSTIIQAPDVLNPSPV
                     IPFPGRANIVDIFNEAIVTMKGFTIAGPSGNICEGLAGVSIQEDATLEIDSSAIIGCL
                     REGIQVGLSEFIPIGPNVGHAVITNTDITDYRVVGIQTGGENTTLVVSKSQVIAADAL
                     EGEDQIGIFGGIGPKVVIVDNKISGNICKTPECIPDLSPQVPHSGIFLLDVNQDSIIA
                     GNKVTNNDVGIAVAGNSGCCKIENNKLSDNLFFGVIVVDGEHTISNT"
BASE COUNT          750 a          273 c          325 g          712 t
ORIGIN      
        1 attttgagtt aagacatgaa catttcttat attttttatt tacgaataat atgttgaatg
       61 ttttactata attaacttac cttttcctat gaggttatat taaaatagta aatcaattat
      121 ctaatactat gggaaataga agtaggacag aaatagtggg aagtattctt gacgctgcaa
      181 atggaggagc taccaagaca aaaattatgt acatagcttt tctaagtttt aatcaattaa
      241 aggaatacct ttctatttta atagaaagca atctcataga atatcttgat ggaactaaaa
      301 cattcaagac tacagaaaaa ggattaaact tacttaaaat tcataatgaa atgggagaat
      361 tattacaaca aagtaataca atcaatacga atgatttaat ttaatatttt tcattatatt
      421 aaagtatacg cttataattt ttatagtagt tccagtgaat ttattttaaa attaagtctc
      481 taccaataaa tgctcaatca taatattatt tattcgttaa acttaatttg taaattgtat
      541 ttggaatacg atatggttac tagataattg atataactac ctatgagaat gggtattatt
      601 taccttgcat ggacattctc cagataatat atctgtgagc ggatttaact ccacaataac
      661 atttggaatt tatttattat agattgctat tatccgttct ctcttgaaat tctctaagaa
      721 atagaaatga gattactcat attgttatta ttatatatta taaaattaga ttaaaagtac
      781 aacatttttc agtattgatg ttataaataa aaaatagcta ttgaattata caataatcat
      841 tctctaagta aattatatag tatatgttac ttaatgattc taccaattct aaatataatt
      901 agaaagttag ttaacaatat gcataagtcg tctattatag gatttttctt agttagcagt
      961 cttatttttg gaagtggaaa tatgatgata cttcaaggag ctgcagcaca agattattac
     1021 aatgacgata gacagtataa agattcttct tcttcaggat atagagatga taataacgac
     1081 aacaactatt acaacaacaa taactattat ccaccaaaag attttgacaa caacaacaaa
     1141 aaatatgtct gtaaagatgg tccatttgaa ggattgttaa caagttctgt agaattctgt
     1201 aaaccagata taactgttcc aactgatttt ccttctatcc aagctgcaat aaatgcttca
     1261 aatcctggtg acacaataaa agtattacct ggtatttaca ctgaacaaat tataattagt
     1321 aagagtttaa caatcatagg ttcaggtgca aaatctacaa taatccaagc accagatgta
     1381 ttaaatccaa gtccagtaat accttttcct ggcagagcaa atatagttga tatttttaat
     1441 gaagcaatcg ttaccatgaa aggatttacc attgctggtc caagtggaaa tatttgtgaa
     1501 ggattagcag gtgttagtat acaagaagat gcaactcttg aaatagattc ttcggctatt
     1561 attggttgtc ttcgtgaggg tattcaagta ggtttgtcag aatttattcc cataggtcca
     1621 aatgtaggtc atgcagttat tactaatact gatattactg attacagagt tgttgggata
     1681 caaacaggtg gagaaaatac aacattggta gtttcaaaaa gtcaagtaat tgctgctgat
     1741 gcacttgaag gtgaagatca aattgggatt tttggtggta ttggtcctaa agtagtaatt
     1801 gttgataaca agattagtgg aaatatttgt aaaactcctg aatgcattcc tgacttatct
     1861 cctcaagttc cacactctgg tatatttcta ttagatgtca atcaagattc aataatcgct
     1921 ggtaataagg taacaaataa tgatgtaggc attgctgtag caggaaatag cggatgttgt
     1981 aaaattgaaa ataacaaatt atctgacaac cttttctttg gtgttattgt agttgatggt
     2041 gaacatacca tttctaatac
//
LOCUS       DASVEV010000073         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_20932, whole genome shotgun sequence.
ACCESSION   DASVEV010000073 DASVEV010000000
VERSION     DASVEV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20932"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..1258
                     /locus_tag="VF222_03590"
     CDS             569..1258
                     /locus_tag="VF222_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX7141542.1"
                     /translation="MKKKVLFVLTSHDQLGNTGKKTGFWLEEFAAPYYEFIDNGYDVT
                     ISSPKGGQPPIDPKSNMEEWQTDATQRFQKDKSTQEKLANTLVLSKVLATDYDTLFIP
                     GGHGPMWDLATDNNLKKLVEDFYFGGKFVSAVCHAPAGLLQATDKDGNSILKDKKVTG
                     FTDSEESAVHLEKVVPFLLEDRMKELGGKFEKAENFKPFVVTDGKLITGQNPASSFVA
                     AQKVIEMLGKN"
     gene            1560..2267
                     /locus_tag="VF222_03595"
     CDS             1560..2267
                     /locus_tag="VF222_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7141543.1"
                     /translation="MTYEHTQLHHQEIIRITDDEQNVIEGNLTVPKTIDMDVKGITIF
                     AHGSGSSRHSPRNKYVAKVLNDAGIATLLIDLLTSKEEEVDDITLEHRFNIKLLAKRL
                     LTATDWISQYGEFRNLKIGYFGASTGAAAALVAAAERASLVYAIVSRGGRPDLAGSDI
                     LNRIESPTLLIVGEKDTQVIELNERALKQLIRIEKKKKIVIIPKATHLFEEPGTLEEV
                     AKVASGWFQCFFLENKK"
     gene            complement(2501..2647)
                     /locus_tag="VF222_03600"
     CDS             complement(2501..2647)
                     /locus_tag="VF222_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141544.1"
                     /translation="MPVKLSTTRGKIHNKHNSKNIEIINDFLVYMRSNGSSEHHQNNN
                     LKKI"
BASE COUNT         1041 a          383 c          466 g          967 t
ORIGIN      
        1 acttcaaaag ttcgatatac ttggaagaga caagctagca gtatatcaaa ttgatcaatc
       61 cttagcctcg attagaactc agattatgac taaaacaaca gatcaacaat aacaacctcc
      121 aactagctag ttgaggctgg agaatattta gatttctaat cttagataat actttttttt
      181 atatatcttc aactttaaac aactgtgtta gttccaatat taaattaaaa aattcttaaa
      241 aaagtattaa tagaattgtt atatgactat aattaatata ctatttatta tggcataatt
      301 gtataatgga atgaaatatc aacgatctta ttattcatac catcataact gaaaagacta
      361 cattctatat caaaagacta cattctatat ctaaaaagag agataatgcg attttatagc
      421 cttctgaatt tatggtatat caaagttaat acaataagaa atttgattgt tctgaattta
      481 aattttatac aatcaataac cgataaataa tacaaaaaat gaattttatt acatctttaa
      541 tatataatag aaagaaaata attattatat gaagaaaaaa gtactctttg ttttgacttc
      601 tcatgatcag ttaggaaata ccggaaaaaa aacaggattt tggttagaag aatttgctgc
      661 tccctactat gaatttattg ataatggata tgatgtaaca atttcttctc caaagggagg
      721 acaacctcca attgatccaa aaagcaatat ggaagaatgg cagactgacg ctacacaacg
      781 ttttcaaaaa gataaatcca ctcaagaaaa gttagcaaat acattggtat taagtaaagt
      841 tttagctaca gattatgata cattatttat tccaggaggt catggaccta tgtgggattt
      901 agcaacagat aataatttaa aaaaattagt agaagatttt tattttggtg gaaaatttgt
      961 tagtgccgta tgtcatgcac ctgcaggatt attacaggct acagataaag atggaaactc
     1021 aatattgaag gacaaaaaag taacaggatt tacagacagt gaagaaagtg ctgtacattt
     1081 agagaaagta gttccatttt tgttagaaga tcgcatgaaa gaattaggtg ggaaatttga
     1141 aaaagctgaa aattttaaac cctttgttgt aactgatgga aagttgatta caggtcagaa
     1201 tcctgcatcg tcatttgttg cagctcaaaa agtaatagaa atgttaggaa aaaattaaga
     1261 tgatttcttg aattaagcat aacagaataa atttgttata agatatgtta tacccttaat
     1321 actctaattc tgtttcaata ggacacttta attaggaatg caaaacaaat aatcattatt
     1381 gtgtacttat ttgggtatcc aaaaaattag aaattttata gtaggcttac ttctagtctg
     1441 atctgtttaa ttcagatttt taattttatt atttcgatta tttatgagta tatggataac
     1501 aaaaagaata tagatctaaa agtaaataaa tcaactaata gtaatataat gttaaggaat
     1561 tgacatatga acatacgcag ctacatcatc aagaaattat aagaataaca gatgatgagc
     1621 agaatgtcat tgagggtaat ctaactgtac ctaaaactat tgatatggat gttaaaggaa
     1681 ttaccatatt tgctcatgga agtggaagca gtcgccatag cccacgaaat aaatatgttg
     1741 ctaaagtatt aaacgatgct ggtattgcta ctttgctaat tgaccttctt acctctaaag
     1801 aggaagaagt agatgatatt actctagagc atagattcaa cattaaatta cttgctaaac
     1861 ggcttcttac tgcaactgat tggatatcac aatatggtga atttagaaat ctaaagattg
     1921 gatattttgg tgctagtaca ggagcggcag ctgctttagt cgcagcagcg gaaagggcta
     1981 gtttagtcta tgctattgta tcacgtggtg gtagacctga tttggccggt tcagacattc
     2041 tgaatcgcat cgaatctcca acacttttga ttgtaggcga aaaggatacg caagtaatag
     2101 agctaaatga aagggcattg aagcaactta ttaggataga aaaaaagaaa aaaattgtaa
     2161 ttattcccaa agctacccat ctatttgaag agccaggcac gttagaagag gttgcaaaag
     2221 ttgctagtgg gtggtttcag tgcttctttt tagagaacaa aaaataagga taaacataaa
     2281 ctattattgt gtaaatattc ttctaggtta ttatctagaa acattaggct atatttaatt
     2341 tgatatgaaa agcaatgatg gtatatcatt ggccctttat tattattctt gacaagagac
     2401 tatctttcaa agaaatcgta gcaattactc tcattctgtt tttattttga tttcaatagt
     2461 cttaatattt tctagataaa cagctattga cgttatattt tcaaattttc tttaaattat
     2521 tattttgatg atgttcagag gaaccattac ttctcatata tacaagaaag tcatttatta
     2581 tctctatatt ctttgaatta tgtttgttgt gaatctttcc tcttgttgta gataatttta
     2641 ccggcattta tccttctttt ctccttatgt attctcaatc cataatatcg aactaaaagt
     2701 atatgaacca acttatagaa tggggccagt ggaatgtgaa acaaatttga tctaaattat
     2761 ggtccctcta catattctta ttattagttt aaatttttag taacgtatta gtgagtatgt
     2821 ttgtatattt ctctttgcat cttatccgta tagaatt
//
LOCUS       DASVEV010000074         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_22878, whole genome shotgun sequence.
ACCESSION   DASVEV010000074 DASVEV010000000
VERSION     DASVEV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22878"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1552
                     /locus_tag="VF222_03605"
     CDS             488..1552
                     /locus_tag="VF222_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141545.1"
                     /translation="MVGIHVLIVNLLLKERYNVNKHIDRKHPRSEIPSNLLSDSYQDT
                     NSEYLRHRTEYTEKYLNQFKPLPPTYFNSWISNSNHATSISGSGLNTSRRKNNFYSTI
                     REFLQYFQIIHNIQSKNSRIHRMNFNPANLILNLNSSPIDNNFRPYNSISNIGIENTV
                     LFRIYRCKNCSLNIPSEFYGLDQIKSSSEDIHCILCRSQKQPQKQNSDLFNQKIMEWF
                     RTNILSKVDDSKIYLKSINIPQDIYVKLKVERRPHFYYENDDSNIPPWLINLMLTEEF
                     VDLGIIEEDKWAYRLTNSDEKSIEITRDELIEYLNCGNSTTFGLFKFQKNNITEYFFS
                     YIQFERNLDTLEENNLSLID"
     gene            2332..2481
                     /locus_tag="VF222_03610"
     CDS             2332..2481
                     /locus_tag="VF222_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEX7141546.1"
                     /translation="MVKDWNKIKAFVIFEAKCAKCGNDTQVNFRPDGRRPIYCKDCYR
                     KYKKT"
     gene            complement(2638..2711)
                     /locus_tag="VF222_03615"
     tRNA            complement(2638..2711)
                     /locus_tag="VF222_03615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2675..2677),aa:Thr,seq:cgt)
     gene            2797..>3965
                     /locus_tag="VF222_03620"
     CDS             2797..>3965
                     /locus_tag="VF222_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141547.1"
                     /translation="MKISISGARGMYGKDLDLHEIYRLSCLFGSYLKKSKNKKIKCLV
                     ACDTRASGDIIKTIVKSSLMEQNIDVYDLSIAPTPIVFKESKKFDGSIIITASHNPFD
                     WNGLKFIIDGRGIFEKELYELLSTQTMFPIMSYGVSRNFLSNYENDILEFIQKSKELN
                     SYNNKRIGIDAGGGAACGYANNILKNIGHVVYDVSGNKGIFSRGPDPTVDDLLQLKSI
                     VKSNDLDYGFAFDLDGDRLVVVNNNCEKLNSDITLLLCIANILYNFQGKEFVTSIDTS
                     LSIQNFVKNHGCYLTYSKVGEANVVQKMIEKNANAGGEGSSAGFIMPNFNMCRDAILA
                     SVLISTINKKILKECFEISDNYSQIRTKIQIDSKLQKTILEKLYDEFKKDSTEINT"
BASE COUNT         1544 a          500 c          585 g         1336 t
ORIGIN      
        1 cgatactatt taaatataat attgtttgtt cgttaaatca gtcagatata ctaaactatt
       61 tcctttaaat catatctgta tattagaatt atggaaatct atagatgtaa cttttactgc
      121 ttttaagtat tttaatttca actgtgatta ataatttaca taaaaattgt atgttagcaa
      181 ttattactta taagatatat cttttctcat cctaaaaata atatgaaatt gtcactattc
      241 gtccgattca ctgtggtata actgcatcag ttgttgttac tgcagttcct gcagttactg
      301 cagctcctgc agcaaattta taaatgaatg caagcatgta aaagtatatc cttgtatgaa
      361 tgtccttatt gtaaacttac ttctaaaaga agatataatg taaacaagca cattaataga
      421 aaacatccta gatcagaaat accttctaat ctgctatcag atagctatca agatactaat
      481 tccactggtg gtaggtatcc atgtccttat tgtaaactta cttctaaaag aaagatataa
      541 tgtaaacaag cacattgata gaaaacatcc tagatcagaa ataccttcta atctgctatc
      601 agatagctat caagatacta attctgaata ccttcgtcat agaacagaat atacagaaaa
      661 gtacctaaat cagtttaaac cactacctcc aacctatttt aattcttgga taagtaattc
      721 aaatcatgca actagtatat ctggttcagg tttaaatacc agtagaagga aaaataattt
      781 ttattccact attagagaat ttttgcaata ttttcagatt atacataata tccagagtaa
      841 gaatagtagg atacaccgta tgaattttaa cccagctaat cttatattga atttgaatag
      901 ttccccaata gataataatt ttagaccgta taactccata tcaaatattg gtatcgaaaa
      961 caccgttctt tttaggatct acagatgcaa gaattgctct ttaaatattc ctagtgaatt
     1021 ttatggtctc gatcaaataa aatcatccag tgaagatata cattgtatct tatgtagatc
     1081 acaaaaacaa ccacaaaaac aaaatagcga tttatttaat cagaaaatta tggaatggtt
     1141 tagaaccaac atattgtcaa aggttgatga ttctaaaata tatttgaaat ctataaatat
     1201 tcctcaagat atttatgtca agttaaaagt agaacgaaga ccgcattttt attatgaaaa
     1261 tgatgatagt aatattcctc catggctgat aaacttaatg ttaactgaag aatttgtaga
     1321 tcttggaatt attgaggaag ataaatgggc ttaccgatta acaaacagtg acgaaaaaag
     1381 tatagaaata acaagagatg aattgattga atacctaaat tgtggaaatt caacaacgtt
     1441 tggactattc aaatttcaaa aaaacaatat tactgaatat ttctttagtt atattcaatt
     1501 tgaaagaaat ttagatacat tggaagaaaa taatctttca ctaatagatt aggtagtgtt
     1561 ttattataat attaataaca ttatatgtaa caaaatgaat atttgcttaa tttaattatt
     1621 tttgtttttc tgggtcggtt cctgaaaatg ccattatcaa atacgagaaa gtaaatgttc
     1681 aaaaaaatgt atacataacc atccatattt ttatgtatat tatttgggat tcaaatacaa
     1741 aaaaattgaa gaaaaagtat attggtaaag gactgccaga accatttaac ttcacaataa
     1801 cctattatat acaatagatt gcttagagca acttttcaga gagtttctgt cacgattttt
     1861 tacctacaga taaacaaaat ttatttactt tttcgcatta aaaaaaatat taattaataa
     1921 atttacataa tttgcttaca caaaaaataa ttttgggctt taaatccatt attttagaat
     1981 ttattaagcc ataatagtag attagcatgg tagataaaaa aaggtatcaa agatataaaa
     2041 atactaacag gattatatga taacgaaaat tctttcaatg aaaaattgtt acaaaaattc
     2101 aagacatata aacaagaatt acaaaaagat ggaattaatc tagagatgaa agtagttgca
     2161 acaatagaag gacacaatga gtagctcatg acagatttct ttttaggaaa aaatatcaag
     2221 tttaatgtag tgtcttttac tttacttcaa aattgaagat tttctgaaat aaaagaaaca
     2281 caaaatgaga tttcatttga tgaatattgg aatgattctg attcatttga tatggtaaaa
     2341 gattggaata aaatcaaagc atttgttata tttgaggcaa aatgtgctaa atgtggaaat
     2401 gatacccaag ttaattttag accagatggt agacgaccta tatattgtaa agactgttac
     2461 agaaaatata aaaaaacatg ataagattat tgtaatatga tattgtatga gtaattagtt
     2521 attccttaaa atttgattag taattattcc ttaaaacttt aggaaacaat taatatttta
     2581 aaaaccattg ttacagcatg aattcatcaa aagagcggat tcgaactgag cactcaaagc
     2641 ctcaggtggg atttgaaccc acgacctaag gtttacgaaa ccttcgctct accgagctga
     2701 gctacagagg cagtattaac aaaagaaatt tatttttact gatatataat ttagattcta
     2761 gtaacacaat taaatcaatc taactattcg tattgtatga aaatatcaat ttctggagcg
     2821 agaggtatgt atggtaaaga tcttgatctt catgaaattt atagattatc ttgtcttttt
     2881 ggatcttatc ttaaaaaatc taaaaataaa aagataaaat gtctagttgc ttgtgatact
     2941 cgagcttcag gtgacatcat aaaaacaatt gtaaaatcaa gtttaatgga acagaatatt
     3001 gatgtttatg atttaagtat agcacctact ccaatagtat ttaaagagtc caaaaaattt
     3061 gatggatcta tcattataac agcatcgcat aatccttttg attggaatgg acttaaattc
     3121 ataattgatg gacgtggaat atttgaaaaa gaattgtatg aattattatc aactcagaca
     3181 atgtttccaa ttatgtcgta tggagtttct aggaatttct tatctaatta tgaaaatgat
     3241 atactagaat ttattcaaaa gtcaaaagaa ttgaattcat acaataacaa acgaattggc
     3301 atagatgctg gaggtggtgc tgcatgtgga tatgctaata atatattaaa gaatattgga
     3361 catgttgttt atgatgttag tggaaataaa ggtatattct ctagaggccc tgatcctaca
     3421 gtagatgatt tattacaatt gaaatctatt gtaaaatcaa atgatcttga ttacggattt
     3481 gcatttgatc tagatggaga tcgtttagtt gttgttaata ataattgtga aaagttaaat
     3541 tcagatatta cgcttttact gtgtatagca aatatactat ataatttcca gggaaaagaa
     3601 tttgtaacaa gtatagatac aagcttatct attcaaaatt ttgttaaaaa ccatggatgt
     3661 tatttaactt attctaaggt aggtgaagca aatgttgttc aaaaaatgat agaaaagaat
     3721 gctaatgcag gaggggaggg aagtagtgca ggattcatca tgcctaattt taatatgtgt
     3781 agagatgcca tattagctag cgttctaatc agtacaatta ataaaaaaat attaaaagaa
     3841 tgttttgaaa tttccgataa ttattctcaa attagaacta aaattcaaat tgattctaaa
     3901 ttacaaaaaa ctattttgga aaagttatat gacgaattta aaaaagattc aacagaaatt
     3961 aatac
//
LOCUS       DASVEV010000075         7691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_412496, whole genome shotgun sequence.
ACCESSION   DASVEV010000075 DASVEV010000000
VERSION     DASVEV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412496"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..269)
                     /locus_tag="VF222_03625"
     CDS             complement(45..269)
                     /locus_tag="VF222_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HEX7141548.1"
                     /translation="MGNNPEFGLAVMNRVIKKAGAERVSDEASDTLRTILEEIANNIS
                     SNAIDLANHAGRKTIKSDDILLAFKNTYKK"
     gene            complement(334..621)
                     /locus_tag="VF222_03630"
     CDS             complement(334..621)
                     /locus_tag="VF222_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141549.1"
                     /translation="MGLSLSDKKELESLLKFHSLNINQMISFSKHLIPSQGNIEDFAY
                     GFIVGTIIGNYSEKFFQRHGRTPDNDELMDIYFTMSLRSAKIRKAILNELI"
     gene            695..1279
                     /locus_tag="VF222_03635"
     CDS             695..1279
                     /locus_tag="VF222_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX7141550.1"
                     /translation="MNKHNLYRYMLYLDNPQFLPTDADLILKKSRELTNSIEFIIIRD
                     CRIATMFIELDISLEKKENIDKVSDLLKRISSTKEIIEVKERHLTKVEAINNAIELFN
                     DEKYWWSHEALEMVWKESKGQEKQLLNGLILICAAYVHSQKNENTICFSILERSMVKF
                     FNVKDSNYHGINIEKIKIIITQIVENKKILKFAI"
     gene            complement(1284..2393)
                     /gene="speY"
                     /locus_tag="VF222_03640"
     CDS             complement(1284..2393)
                     /gene="speY"
                     /locus_tag="VF222_03640"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX7141551.1"
                     /translation="MNNKHTFTGKKIDPPDIYPNMSISDLISFYGSTGFNARRLSEAA
                     NMFKKMIDSEATICLTISGAMTPIGLGKSISTLIEKGFIDWIITTGANAYHDLHFAYD
                     LPVRQGHFDVDDDILYSKQIVRIYDVYIKELETLQRQDYLIQSIIKKMSKSKDYSVKD
                     ISTADLSYKLGESAYNHSSLPDKSFLVSAFKHKLPVYIPSLTDSSIGMNMIPLLFESS
                     LPSLNLLLDIAESAAILWKSKISGGIELGGGVPKNFFQQTGPTLYQILKIKEGGHDYV
                     IQLTDARPDTGGLSGATIQEGKSWGKIKTSHTGNVIVYGDSSVYFPLLCSFVLSECKP
                     RKPKKLYQLKDEFVKEMKDNYLKKKIRKKKKVNEP"
     gene            2492..3427
                     /locus_tag="VF222_03645"
     CDS             2492..3427
                     /locus_tag="VF222_03645"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX7141552.1"
                     /translation="MNYNKNKKFANYDIRDYSIEKGDLKEVTNQMSKSGGFESVNLTD
                     GITILKNMIAEPNCTKFLSFVGAIISTGVRGIIKDMIKNNMFDCIITTCGSLDHDIAR
                     SFGKYYSGDFNVDDYHLKKRNIHRLGNVFIPVENYGPLIETKIQEYIYEFYKDNRQFA
                     SHEFIDYIGSKLGETSFLYWAHKNKIPVIVPGIVDGAVGSQIWLFYQNHKDMVLNILK
                     DQTKISDIIFEAKKTGALMLGGGISKHHTLWWNQFRDGLDYAVYITTANAWDGSLSGA
                     EVKEAISWNKVKSEAKQVTIHGEITTLLPFIYSSI"
     gene            3603..6398
                     /locus_tag="VF222_03650"
     CDS             3603..6398
                     /locus_tag="VF222_03650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7141553.1"
                     /translation="MFSGNKTLFRVKSFPFQAKHLLVILVLIIAFSTAFIIRSYPIKY
                     GFALNEFDPYFDYRATKYIVDNGFDAYLKWHDYQSWYPEGRYVPTTSQVALHLIAATM
                     YKIFGFGSSLLDFVIMFPVIIGSLTTIVIFALVRNLSGTTAGMFAGLLFAFMPAIIQR
                     GNLGWFKSEPLGLFLALLSVYLFISSIKSKNILRTILTAAGGGLILGFANSSWGGIQY
                     FSIPLAIFFFALPFLRKDSIFLYGIITFTLITLVTAGAFPRPGISFVIGLPGIALIVG
                     MIFAIVGTIINTKSSAAKANRNLIAALGIFILLTVSIFVVGAYQPSSFRYLVAVNPFL
                     SSHNPLVESVAEHSTPTVIDYFTDYSILLIFAGFGVWVSFSKRTDMTIFALIIGLTGL
                     YVSATFARLMVYSSIAIVILASIGLYELTRSILQNRIENQDKIKEKEFRKNIKEGKIL
                     PDVKPLVKISFVAMIVFLVSIPLIDYNGLIYPKNFNWLSSADIPPSIVNGATGFRMQV
                     EDWVDAVDWIADNTPKDSVIASWWDYGYWITTLGNKTTLADNATINQTRIQTIAKMLM
                     SEQETATQIAHDLKADYILVYIVADRHNGPNGNSFYTLGSGGDESKKQWFMKIGGFTE
                     RDYVEDDGFTPNSNFWNNTLLGKLIPFTPISYASFGNGMLTNIQPEYSSGTIALNLKD
                     IKYPANSTNDLPYKLVYASPSFENDNEDIVFGVFIYQVNKNYKPNPISQEAITKANAT
                     KDKESNTADERAQNMTKANMTPSDNVAILKTSLGNITIEFFPQAAPKHVNNFLNLSKT
                     GFYDGTIFHRIVKDFVIQGGDPTTKNTTQKDRWGTGGPSYTLDAEFNDIPHERGILSM
                     ARTADPNSAGSQFFIITKDSRFLDNQYTVFGRVIDGMETVDKIEALTTNQSSDQPIDF
                     EKAQIQKVIIEPRKDTK"
     gene            6438..6812
                     /locus_tag="VF222_03655"
     CDS             6438..6812
                     /locus_tag="VF222_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141554.1"
                     /translation="MNELESVILGAQRKLLVICDELDLSAISSSNQVFILLVEESKGS
                     AGGRGGGSGHRKIAQVIGFKIENKKIEKIYDTKDENIIEKFEIPYSAIAIDIKLSDGR
                     QIVVQGIIDQEMIKNYNNIIFS"
     gene            6882..7040
                     /locus_tag="VF222_03660"
     CDS             6882..7040
                     /locus_tag="VF222_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141555.1"
                     /translation="MNNNKKLAGGEIIGIGTDFLVIHKEGKRFLIEVDFESLKELKLS
                     TTPYGSQI"
     gene            7109..>7691
                     /locus_tag="VF222_03665"
     CDS             7109..>7691
                     /locus_tag="VF222_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141556.1"
                     /translation="MKINSKLELKYQKKDTDNISPHHLNKNPKVKYNKKAESEFFVDN
                     SALAGFTGERILYMAIRELIENSLDSCEVHHILPNISLSIYQTDAGNDLWTISCQDNG
                     IGINSEKVPVAVCSFLTSGKYVEKQQRGLFGVGLKMIAAFSTKDTDKPIKVWSKSIDE
                     DSEYYYELRTDITTNKPIVLNKKIVPKTENTIKD"
BASE COUNT         2697 a         1161 c         1277 g         2556 t
ORIGIN      
        1 tttgactgtt taaactcatt aaaataatct atgttaaaat ctattcattt tttataggta
       61 ttcttaaagg caagtaatat atcatcagat ttaattgttt ttctgccggc atgatttgca
      121 agatctatag cattactaga tatattgttt gcaatttcct ctagtattgt tcttaaagta
      181 tctgatgctt catcacttac tctttctgca ccagcctttt ttattactcg attcattaca
      241 gctaatccaa attcagggtt attacccata atataaaaat gctcagcata gttaaaaatc
      301 taattgatat tatacataat aatacgcaaa atattaaatt aattcattta atatcgcctt
      361 cctaatttta gcagatctca atgacattgt aaaatatata tccattaatt catcgttatc
      421 aggagttcta ccgtgtctct gaaaaaattt ttcagaatag ttacctatta ttgtgcctac
      481 aataaatcca tatgcaaaat cttcgatatt accttgcgat ggaataagat gtttagagaa
      541 acttatcatc tgatttatat ttaaagaatg aaattttaat aatgattcaa gttccttttt
      601 atctgataag ctgagtccca aaattagttt aatctagcct tgatatatat ttttaattat
      661 tatatttatt agcaaacatt gaggaattat agaaatgaat aagcataatt tgtatagata
      721 tatgttgtat ttagataatc cacaattttt accaacagat gccgacttga tcctaaaaaa
      781 atcaagggag ctaactaatt ccatagaatt tattataatt agggattgtc ggatcgcaac
      841 catgtttatt gaattggata tcagtcttga aaaaaaagaa aatatagata aggtatcgga
      901 tttgttaaaa aggatttctt caacgaagga aattattgaa gtaaaggaaa gacatttaac
      961 caaagtagag gcaattaata atgcgataga actttttaat gatgagaaat attggtggtc
     1021 tcatgaagca ttagaaatgg tttggaaaga atcgaagggt caggaaaagc aattactcaa
     1081 tggattaata ctaatctgtg ctgcttatgt tcactctcaa aaaaatgaaa atactatatg
     1141 tttttcaata cttgaaagat caatggtaaa attttttaat gtaaaagatt caaattatca
     1201 cggtataaat attgagaaaa taaaaataat aataacgcaa atagtagaaa ataaaaaaat
     1261 tctaaagttt gcgatttaaa gcttcaaggt tcattaactt tctttttttt tcttattttt
     1321 tttttcaaat aattatcctt catttctttt acaaattcat ctttcaactg gtacaatttc
     1381 tttggttttc gtggtttaca ttctgacaaa acaaatgaac ataagagtgg aaaatagaca
     1441 gatgaatctc catacacaat tacattgcca gtatgagatg tttttatttt gccccaactt
     1501 ttcccttctt gaatcgttgc accagataaa ccacctgtgt caggtcttgc atctgtcaat
     1561 tgaataacat aatcatgacc tccttctttt atttttaaaa tttgatataa ggtaggaccc
     1621 gtttgttgga agaagttttt tggtactcct ccacctaatt ctatacctcc tgaaattttg
     1681 cttttccaca atattgctgc tgattcagca atatctaata gcaaatttag actaggtaag
     1741 gatgactcaa aaagaagtgg tatcatattc attccaattg atgaatctgt cagggatggg
     1801 atatataccg gcaatttatg tttaaatgca gaaaccagga aagatttgtc aggcaacgaa
     1861 gaatggttat atgcagactc tccaagttta taagaaagat cggctgttga aatatctttt
     1921 actgaataat ctttactttt actcattttt tttattatgc tttgtataag atagtcttgt
     1981 ctttgaagtg tttctaattc tttaatatat acatcgtaaa ttctaacgat ttgtttagaa
     2041 tataatatat catcatccac atcaaaatga ccttgcctga ccggtaaatc ataggcgaaa
     2101 tgaagatcgt gatatgcatt agctcccgtt gtgataatcc aatcaataaa gcctttctcg
     2161 atcaaagttg aaatagattt gcctaagcca attggagtca tagctcctga aatagttaaa
     2221 caaatagtgg cttcactgtc aatcattttt ttaaacatat tagctgcctc tgataatcgc
     2281 ctagcgttaa atccagtaga cccatagaaa gaaataagat cggaaataga catatttgga
     2341 tatatgtctg ggggatcaat tttttttcct gtgaaagtat gtttattatt cacatgacat
     2401 gatacaatta cctgactttt ataaaatgcg aatataaagt taattacaag taaatatatt
     2461 cttatattga tactattttt attacttttc aatgaattat aataaaaata aaaaatttgc
     2521 taactatgat attagagatt atagtattga aaaaggtgat ttaaaggaag tcacaaatca
     2581 aatgtccaaa tcagggggct ttgaatctgt aaatttgact gacggaatta ctatattaaa
     2641 aaatatgata gcagaaccta attgtactaa attcttatct tttgttggag caataatctc
     2701 tactggagta cgaggaatta ttaaagatat gataaaaaac aatatgtttg actgtataat
     2761 aactacatgc ggttctttag atcatgatat agccagatct tttggtaaat attactctgg
     2821 agactttaat gtggatgatt atcatcttaa aaagagaaat attcacagat taggaaatgt
     2881 attcattcct gtagaaaatt atggtccttt aatagaaact aaaatacaag agtatatcta
     2941 tgaattttac aaagacaaca gacaattcgc tagtcatgaa ttcattgatt atataggaag
     3001 taaattagga gaaacatctt tcttatattg ggctcacaaa aacaaaatac ctgttattgt
     3061 ccctggaata gttgacggag cagtaggaag ccagatatgg ctattttacc agaaccacaa
     3121 ggacatggtt cttaatattc taaaagatca aacgaaaatt tctgatatca tatttgaggc
     3181 aaaaaaaacc ggtgctttaa tgttgggagg tggaatatct aaacatcata ctctatggtg
     3241 gaatcaattt agagatggat tagattatgc tgtttatatt acgactgcaa atgcttggga
     3301 cgggagttta agtggagcag aagtgaaaga agcaatttca tggaataagg taaaatcaga
     3361 ggcaaaacaa gttactatac atggtgaaat aaccacatta ttacctttta tctattcctc
     3421 tatttgaaaa tgaacagttt ttcttaggta gttgtaaaaa tagaaagatc attgtctgaa
     3481 tatctaaatg ataatgacta ttctggtttt ttatgttatt ggtattattt tggactaaat
     3541 tcgtttttta attaaaatag ccattacctt aaaaaggaag taaaaaatag tagtttatta
     3601 cattgttctc tggcaataaa acattatttc gagtaaaatc ttttccgttt caggcgaaac
     3661 atctccttgt cattctggtt ctgataatag ccttttctac cgcatttatt atcaggtcct
     3721 atccaataaa atatggtttt gctttaaatg aattcgatcc atattttgat tacagggcca
     3781 ctaaatatat tgttgataat gggtttgacg cttatttaaa gtggcatgac taccaatctt
     3841 ggtatcctga aggaagatac gtacccacga cttcacaagt tgctcttcat cttatagcag
     3901 caaccatgta taaaattttt ggttttggta gttcactttt ggattttgtt ataatgtttc
     3961 ctgtcatcat aggatcactg acgactatag ttatttttgc tcttgtacga aacctaagtg
     4021 gtactaccgc agggatgttc gctggtctgt tgtttgcttt catgcctgca ataattcaga
     4081 gaggcaattt gggatggttt aaatctgaac ctttagggct tttcttagca cttttgtctg
     4141 tttatttgtt tatctcctcc ataaaaagca agaatatcct acgaactatc ctaacagcag
     4201 caggcggagg gctaattctg ggatttgcca attcctcatg gggtggcatt cagtatttca
     4261 gcattccatt ggcaattttc ttttttgcgt taccttttct tagaaaggat tcgattttcc
     4321 tttacgggat tattactttc acgttaatta ctttagtaac agccggcgcg tttcctcgcc
     4381 caggtatatc atttgtcata ggacttcctg gaatagctct tattgttgga atgatatttg
     4441 caattgtcgg taccattatt aatacaaaaa gttctgctgc aaaagctaat cgaaatctta
     4501 ttgcagcact tggtatattt attttactta ccgttagtat atttgttgta ggggcttacc
     4561 aaccctcatc atttcgatat cttgttgcag taaatccctt tctatcttct cataatccat
     4621 tggtcgaatc agttgctgag cattcgacac caacagtaat cgattatttt acagattact
     4681 caatattatt gatatttgca ggatttggag tttgggtttc attttctaaa agaactgata
     4741 tgaccatttt tgccttaatc ataggactaa ctggtctgta tgttagtgca acttttgcaa
     4801 ggctaatggt ctattcttca atagcaattg taatacttgc ttctattggg ctttatgaat
     4861 taacaagaag cattcttcaa aacaggattg aaaatcagga taagataaag gaaaaggaat
     4921 ttaggaaaaa tatcaaagaa ggtaagattc taccagacgt gaaacctctt gtcaaaatat
     4981 cctttgtggc aatgattgta ttcttagttt ctattcctct gattgattat aatgggctta
     5041 tatatccaaa gaattttaat tggctcagtt ctgcagatat tcccccatca atagtaaatg
     5101 gagcaacagg ttttagaatg caggtagagg attgggtaga tgcggtagat tggattgctg
     5161 acaacacacc caaggactca gtaatagcgt catggtggga ttacggatat tggataacca
     5221 cgttaggaaa caaaaccact ttagctgata atgcaacaat caatcaaaca aggatacaaa
     5281 ctatagctaa aatgttaatg agtgaacaag aaacagcaac tcaaattgct catgatctaa
     5341 aggctgatta tatattagta tatattgttg cagataggca taatgggccc aacgggaatt
     5401 cgttttacac tttaggaagt ggaggcgatg agagtaaaaa gcaatggttc atgaagattg
     5461 gtggtttcac tgaaagagat tatgtagaag atgatggatt tacccctaat tccaatttct
     5521 ggaacaacac tttgttagga aaattaattc ctttcacccc catttcttac gcatcgtttg
     5581 gtaatggcat gttaactaac attcaacccg aatattccag tggaacaatc gctctaaatc
     5641 tcaaagatat aaaatatcca gcaaactcaa caaatgatct accatataaa ctggtgtatg
     5701 cctctcctag ctttgaaaat gacaatgagg acattgtttt tggagtgttc atttatcaag
     5761 ttaataaaaa ttacaaacca aatcccatct ctcaagaagc cattacaaag gctaatgcaa
     5821 caaaagacaa agaatcaaat acagcagatg aaagagctca aaatatgact aaggccaata
     5881 tgacaccatc tgataatgtt gcgattttaa aaacctctct tggaaacatt acgatagaat
     5941 tttttccaca agccgctccc aagcatgtta acaattttct aaatctatca aaaaccggtt
     6001 tttatgacgg aactattttt caccgaattg taaaagattt tgttatacag ggaggtgatc
     6061 caactaccaa gaataccact caaaaagata gatggggaac cggaggacct agttacactc
     6121 ttgatgctga atttaatgat atccctcatg aaaggggaat attatctatg gcgagaaccg
     6181 ctgatcctaa tagtgccggc tcacaatttt ttataattac gaaagattca aggttcttgg
     6241 acaatcaata taccgttttt ggaagagtca ttgatggaat ggaaacagtt gataaaatag
     6301 aagctcttac gacaaatcaa tcttctgatc aaccaataga tttcgaaaaa gcacagattc
     6361 aaaaagtcat aatagaacca agaaaagata ctaaatagaa aattgatgtc tcttcgatta
     6421 tatccttaga atgaagtatg aatgaattag aatcagtcat cttaggcgct caaagaaaac
     6481 tgttagtaat atgtgatgaa ttagatttat cggctatttc atcttccaac caggttttta
     6541 ttttactagt agaagaatca aaaggatcag caggaggtag gggaggtggt tcgggacatc
     6601 gtaaaatagc tcaagtaatt gggtttaaga ttgaaaataa aaaaatcgaa aaaatatatg
     6661 atactaagga tgagaatata atagagaaat ttgaaatacc ttatagtgca atagcaatag
     6721 atatcaaatt atctgatgga agacaaattg tagtacaagg aattatagac caagagatga
     6781 ttaaaaatta taataacata attttttctt aatcatcaag ataatatgat tataagaaaa
     6841 aattataata agagtttaac tcaatatcat agaaaacaat aatgaacaac aataaaaaac
     6901 ttgctggagg agaaatcatc ggaataggta ctgattttct ggttatccat aaagagggaa
     6961 aaagattttt aattgaagtg gattttgaat ctttaaaaga gcttaaactt tcaactactc
     7021 cgtatggatc acaaatataa tatattccga agactattat ttttaatcct aaaagataaa
     7081 tttagttatt ttataaaagt catatcctgt gaaaataaat tctaaattag aactgaaata
     7141 tcaaaagaaa gatacagata atatttctcc ccatcatctt aacaaaaatc ctaaagtaaa
     7201 atataataaa aaagctgaat cagaattttt tgtagataat tctgctttag ctgggtttac
     7261 tggggaacgt attttataca tggcaataag agagttgata gagaattcac tagattcctg
     7321 tgaggttcat catattcttc ccaatatttc tttatcaata tatcaaactg atgccggaaa
     7381 cgacctctgg acgatctctt gtcaagacaa tgggataggg atcaattctg aaaaggtacc
     7441 cgtggcagtc tgttcttttc taacatctgg taaatatgtt gaaaaacaac aaagaggatt
     7501 atttggagta ggacttaaaa tgattgcggc attttccaca aaggatacag ataaacctat
     7561 aaaagtgtgg tcaaaatcta ttgatgaaga ttcagaatac tattatgaat tgagaacgga
     7621 tattacaact aataaaccta tagttttaaa caaaaaaatt gtaccaaaaa ctgaaaatac
     7681 tatcaaagac g
//
LOCUS       DASVEV010000076         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_23038, whole genome shotgun sequence.
ACCESSION   DASVEV010000076 DASVEV010000000
VERSION     DASVEV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23038"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF222_03670"
     CDS             <1..375
                     /locus_tag="VF222_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX7141557.1"
                     /translation="THGFNTFIPLKFSLENTELEKKKLIVRESPSTYDLRIVAIARLL
                     LKDVLDNISVYWVSLGKKIAQVALTYGGNDLVGTAFSEEIFRAAGKASGISIQELVNL
                     IKEIKREPAQRDTFFNIIEKYE"
     gene            complement(586..1413)
                     /locus_tag="VF222_03675"
     CDS             complement(586..1413)
                     /locus_tag="VF222_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX7141558.1"
                     /translation="MIEITLGHTPDADDAFMFYGLASENVGSNEFHITHVIEDIEKLN
                     KRALNNELDITAISVYAYAFLKDYVILRSGGSFGINYGPIILAKEKFLKDDLNKKIIA
                     IPGKMTSAYLLSSLLLGNFKEKELPFHIIPQAIIDNQVDAGLVIHEIQLAYKSLNLHK
                     IIDLGSWWHNLTNGLPVPLGINVANNKTLTLEQIRRFDSLFKNSIIYGLNNIEEALDY
                     AMKYGRSQSKDMISKFVKMYVNEITVDMGEKGKTSIERIFQLASERNIVSPFQIYFA"
     gene            1489..1782
                     /locus_tag="VF222_03680"
     CDS             1489..1782
                     /locus_tag="VF222_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141559.1"
                     /translation="MNNKNFILQQLKWISIYFAISIVISFIIPFPYSIIVIIPIVLFL
                     SFYIRRREMRKFGGVESFSMFNSNKVNYYCMVCGTKHDNLMCPKCGSKMKRIG"
     gene            1958..2371
                     /locus_tag="VF222_03685"
     CDS             1958..2371
                     /locus_tag="VF222_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141560.1"
                     /translation="MFRKEIYLPFLLSILLFLSIDISFYNYIVLGKEDDSKDNVKEEK
                     EERDDNRDSKDNVKEEKEERDDNRDSKDNVKEEKEEDFEDISLDELFEDNNADDNRDS
                     KDNVKEEKEEKEEKEEKEEEDEVPFLLPFNAVPFP"
     gene            2476..2591
                     /locus_tag="VF222_03690"
     tRNA            join(2476..2501,2518..2531,2547..2591)
                     /locus_tag="VF222_03690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2528..2530,aa:Leu,seq:taa)
     gene            complement(2609..3235)
                     /gene="pdxT"
                     /locus_tag="VF222_03695"
     CDS             complement(2609..3235)
                     /gene="pdxT"
                     /locus_tag="VF222_03695"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX7141561.1"
                     /translation="MSNIKIGVLGIQGDIEENILSTAEALKNLNLQGTVESVRYSESI
                     AELDGLILPGGESTVIGNLLSIQGKVFQTIEKKIIEGMPVLGTCAGLIMLSKRTYDKV
                     VGETKQKLFGMLDVVIERNAFGRQHESFEVDLEIPILGKEPFRGIFIRSPIVNNVGDS
                     VTILTKLSDKIVAVKQNNIIGTSFHPELSADRRLHELFIKDILEFSKN"
     gene            complement(3232..4203)
                     /gene="pdxS"
                     /locus_tag="VF222_03700"
     CDS             complement(3232..4203)
                     /gene="pdxS"
                     /locus_tag="VF222_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX7141562.1"
                     /translation="MTISLVAPEEERNGIKFKNLHNEQLVQGTTLVKRGFAHMQKHGV
                     IMDVTTVEQAQIAEDAGAVAVMVLDKLPYDVRKAGGVARTASIKVIEEIMNEITIPLM
                     AKCRIGHISEAKILEVIGVDMIDESEVLTPADEENHIWKWDYTTPFVNGAKNLGEALR
                     RIEEGSSMIRTKGEPGTGNIAEAIRHIKQVNNEIRKLRSFYFDKDEQELIKFSRELKV
                     SYSLIKETAKLSRLPVVNFAAGGISTPADAALVMNLGCDGVFVGSGIFKAEDPEQRAK
                     SVVLATTYYDDPTIVMEAQKLVDEKKSLLGLDTKSLELRMQERGPMI"
     gene            complement(4306..4932)
                     /locus_tag="VF222_03705"
     CDS             complement(4306..4932)
                     /locus_tag="VF222_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141563.1"
                     /translation="MSNNFGDNSQTSFYLNGSIFSLIFIGDEIHVEDNNNFANLLNLD
                     KEKEDVNFVDFLTNSLIDIISNEGDSSFSLEQVLSNSNITTSNNAVNSKKLLLLNGSW
                     NLDVKNGNIVSFDAKISTINKDGMSPHMYEILNLVVVNGNTPIKLSQSDSTIIEGRAD
                     IITPDNIKNIGLYLILQENSISIILDPKILGNDYNGQPIYGLTNYIKN"
     gene            complement(5101..5460)
                     /locus_tag="VF222_03710"
     CDS             complement(5101..5460)
                     /locus_tag="VF222_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141564.1"
                     /translation="MVSEGSEYDKIYNAICSVIANLKEEGIDIKTKFENESGFIGIYT
                     SNTSILKRAKNGLNEILELSYSTAEHHPYWNIIYNTSEIAKTTLDNWNENFSLDQIQE
                     MLWRNDEIKNTLSRLEK"
BASE COUNT         2052 a          880 c          805 g         2109 t
ORIGIN      
        1 actcatggct tcaatacatt tataccactt aagtttagtc tagaaaatac tgaattagag
       61 aagaaaaaat tgattgttag agaaagtccc tctacttatg acttaagaat agtagccatt
      121 gctagattat tattaaaaga tgttttagac aacatatcag tttattgggt ttctctaggt
      181 aaaaaaatag ctcaggtagc acttacatat ggaggtaatg atttagttgg tacagctttt
      241 tcagaagaaa tatttcgtgc tgcgggaaaa gctagtggta tatcgataca ggaacttgta
      301 aatttgatca aagaaataaa aagagaaccc gctcaacgag atacattttt taatataata
      361 gaaaaatacg aatagtcata caaatcaaca caatatcaga aaaatcacaa aatcggcaat
      421 atctattact ttttatcata atattatatt ttaaacatgt aaaactatat gctcatacta
      481 ttttctcaaa taaattctta tgatgaaaga tttcaagata gttctgattg ttattttaat
      541 taaataagaa ttatatttta atttggtttg ataaatttct ataaattagg caaaatagat
      601 ttgaaatgga gatacaatat ttcgttcaga tgccaattga aagattcgtt caatagatgt
      661 ctttcccttt tcacccatat caacggtgat ttcattcaca tacatcttta caaatttaga
      721 tatcatatct tttgattgac ttcttccata cttcatcgca taatctaacg cttcttctat
      781 gttatttaga ccataaatta tagaattttt aaacaaagaa tcaaagcgtc taatctgttc
      841 tagtgtcaat gttttattat ttgcaacatt gatacccaat ggaacgggca aaccgttagt
      901 aagattatgc caccaagatc ctaaatctat aattttatgt agatttaatg acttgtaagc
      961 aagttgaatt tcatgaatta ctaaacccgc atcaacttga ttgtctatta ttgcttgagg
     1021 tataatatga aaaggtaatt ctttttcttt aaaattacct aacaaaagag atgataataa
     1081 atacgctgaa gtcatctttc caggtatagc tattatcttt ttatttagat catcttttaa
     1141 aaatttctct tttgctaaaa taattggacc ataatttatt ccaaaactcc ctccacttcg
     1201 aagaattaca taatccttaa gaaaagcata ggcataaaca gatattgctg ttatgtctaa
     1261 ctcattgttc aatgcacgtt tattgagctt ttcaatatct tcaattacat gtgtaatatg
     1321 aaattcatta gaccctacat tttcacttgc taatccataa aacataaacg catcatcagc
     1381 atctggagta tgtcctaaag ttatctcaat catttacagt ttttaagata caatgctaat
     1441 aattaaaagt agatcaagat ttatttatat gaaatatata atatagttat gaataataaa
     1501 aattttattt tacaacaatt aaaatggata tcaatatatt ttgcaattag tattgtaatt
     1561 tcttttataa tcccatttcc ctattcaatt attgtaataa ttccaatagt attatttttg
     1621 agcttttaca taagaagacg agaaatgaga aaatttggag gagtagagag tttttccatg
     1681 ttcaattcta acaaggtaaa ctattattgc atggtatgtg gaactaagca tgataatttg
     1741 atgtgtccaa aatgtggctc gaaaatgaaa agaataggat aataattatc atacattttt
     1801 catactcatc aaagaaaatt tgttcataaa tatacaaaac aatttaatga tagtttcata
     1861 tagaaattat ttctattaaa tataccagta ttattatata gtaatacatt acttaaagct
     1921 aatataattt aaaataacat gagtaatagt actacaattg tttagaaaag aaatatacct
     1981 tccattttta ctaagtattt tactattttt aagtattgat atttcatttt ataattatat
     2041 tgtgctagga aaggaagacg atagtaagga taatgtaaag gaagaaaagg aagagagaga
     2101 tgacaataga gatagtaagg ataatgtaaa ggaagaaaag gaagagagag atgacaatag
     2161 agatagtaag gataatgtaa aggaagaaaa ggaagaggat tttgaagata tttctctaga
     2221 tgaattgttt gaagataata atgctgatga taatagagat agtaaggata atgtaaagga
     2281 agaaaaggaa gaaaaggaag aaaaggaaga aaaggaagag gaagatgaag tgccattttt
     2341 acttccgttt aatgcagtac catttccata aattgtgaaa ttaaaatcag caattatatt
     2401 aagatgacaa aggagttatg taataaaatc acatttgtaa agtttaatat cagggttatc
     2461 agacttgcat aatgggcggg ggtcgtagag cccggccaaa taacaaaaaa tttggagacg
     2521 cgggacttaa gatctaagaa tgggtgatcc cgtcctttag aggttcgtgg gttcaaatcc
     2581 caccccccgc atgttagaca aatttaattt aatttttgct aaattctaaa atatctttga
     2641 taaataattc atgtaatctt ctatctgctg aaagttcagg atgaaacgat gttccaataa
     2701 tattattttg ttttacagct acgattttat cactgagttt agttaggata gttacactat
     2761 ctccaacatt atttactata ggcgacctta tgaaaattcc tctaaaaggt tccttcccta
     2821 aaattggaat ttcaagatct acttcgaaag attcatgttg tcttccgaat gcatttcttt
     2881 caattacaac atctaacatt ccaaataatt tttgttttgt ctctccaaca actttatcat
     2941 atgttcgttt tgataacatt atcaaaccag cacacgtgcc taataccggc ataccttcga
     3001 tgattttctt ctctatggtt tgaaagactt taccttgaat agagagcaaa tttccgatta
     3061 cagtactttc accacctggt aatattaaac catctaattc tgctatggat tcagaatagc
     3121 gaaccgactc cacggttcct tgtaagttta gattttttaa tgcttcagct gttgataata
     3181 tattttcttc tatatctcct tgaattccta gtactcctat cttaatatta ctcaaatcat
     3241 aggacctctt tcttgcatac gtaattcaag acttttagta tctagaccta ataatgattt
     3301 tttctcatct actaattttt gagcttccat aactatcgta ggatcatcat aatatgttgt
     3361 agctaatact acggatttag ctctttgttc tggatcttca gctttaaaaa taccagagcc
     3421 aacgaaaaca ccatcgcagc ctaaattcat aactaatgct gcatctgccg gtgttgatat
     3481 accacctgca gcaaaattta ctactggtaa cctagataat ttagcagttt ccttaatcaa
     3541 ggaataagaa actttgagtt cacgggaaaa tttaattaac tcttgttcat ctttgtcaaa
     3601 ataaaaagat cggagttttc taatttcatt gttcacttgt ttaatatgtc gtatagcttc
     3661 tgcaatattt ccagttcctg gttcaccttt agttctaatc atagaacttc cttcttctat
     3721 tcgtcgtagt gcctcgccga ggttttttgc accatttaca aaaggagtag tatagtccca
     3781 tttccatatg tgattctcct catctgcagg tgtaagaact tcactctcat caatcatatc
     3841 aacacctatc acttcaagta tttttgcctc tgatatatgt ccgatcctac attttgccat
     3901 gagtggtatt gtaatttcat tcattatttc ttcaataact ttaatacttg cagtcctagc
     3961 cactcctccg gcctttctaa catcgtaagg taatttatct aaaaccatga ctgctacagc
     4021 acctgcatct tctgcgatct gtgcctgttc tacagtagta acatccataa taacaccatg
     4081 tttttgcata tgagcgaacc ctcgctttac taatgtagtt ccttgaacta attgttcatt
     4141 atgtaaattc ttaaatttaa tgccgttacg ctcttcctct ggtgctacaa gagaaattgt
     4201 cattatttaa aatattaaaa agcatctatt ataggtttag ttttagtatg acctaatatg
     4261 taaagttaaa cagtacccat attggacaaa taattggtta acattctaat tttttatata
     4321 attggttaat ccataaatag gttgtccatt gtaatcattt cctaatattt tggggtctaa
     4381 aataatactt attgaatttt cttgcaaaat taaataaaga ccgatgtttt taatattgtc
     4441 aggagttata atgtctgctc taccttctat aatagtacta tcactttgag ataatttaat
     4501 aggtgtatta ccatttacaa ctactagatt tagaatttca tacatatgtg gagacattcc
     4561 atctttattt attgtagata ttttagcatc aaatgatacg atattcccgt tttttacatc
     4621 taaattccaa ctgccattaa gaagaagtag tttttttgaa ttaacagcat tattagatgt
     4681 agtaatattt gaatttgaaa gtacttgttc taaagaaaaa cttgaatcgc cttcattaga
     4741 gatgatatca attaaactat ttgttaagaa atctacaaaa tttacatctt ctttttcttt
     4801 gtctaaattt aaaagatttg caaaattgtt attatcctcc acatgaattt catctccaat
     4861 gaagattaaa gaaaatatag aaccatttag ataaaaggat gtttgagaat tatctccgaa
     4921 attattagac aaatttaaaa ttggaataga ttctccaaaa tatagaaatt tgaatataac
     4981 caaaaaaact aaagtaatta caaataaaat tataagccat ttcattaaac gactatttat
     5041 attttttaat actattaaat ttatatgatt attgcgacca ataacaaact aaagatgaat
     5101 ctatttttct aatctactga gggtattttt aatttcatca tttctccaaa gcatttcttg
     5161 tatctggtca agactaaaat tttcattcca attatctagt gtagtttttg ctatttcgga
     5221 agtattatat attatattcc aataaggatg atgctctgct gtagagtagg ataactctaa
     5281 tatttcgttt aaaccatttt tagccctctt taaaatcgag gtattagatg tatatattcc
     5341 aataaatcca gactcgttct caaatttagt ttttatatct attccttctt cttttagatt
     5401 tgctataaca gaacaaatag cattgtaaat tttatcatat tctgaacctt cagagaccat
     5461 attttatata attagcttgt tttatttata gaattagaaa ttatagggaa tagttactct
     5521 ataagattta aatatcaaat aaattgtatg aatttttcat attattcctc atccaaatta
     5581 tctatgcttt caaattcatc gtagctttct ttcaagtctt cttcaaattc tgtttccagt
     5641 tcttcattag acataatatt tactaattat aattaaaaca taaatctttt caaaataaat
     5701 tgatgacaat gaaattaaat catataaata attatttata agaaggtata tgattatatt
     5761 aaaaatgaat accacagtca aaaaacctat tggtgataat gaaacttcat gtgattattg
     5821 tggaaatccc atagaaatat gtatgt
//
LOCUS       DASVEV010000077        22544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1383972, whole genome shotgun sequence.
ACCESSION   DASVEV010000077 DASVEV010000000
VERSION     DASVEV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 22544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22544
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383972"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF222_03715"
     CDS             <1..279
                     /locus_tag="VF222_03715"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX7141565.1"
                     /translation="LYLKILNSTNFKTSMSENKGPKMIKDDFYPSFYLKNMKKDLDLV
                     MEAAKEKDLSLPITGIVFQLYNYASRSIFSDLDYTGIYKFLRKLNGLE"
     gene            complement(810..1169)
                     /locus_tag="VF222_03720"
     CDS             complement(810..1169)
                     /locus_tag="VF222_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7141566.1"
                     /translation="MESDIKDESENISFEEFSKVSLRLGKIKKIDYIPKLNKVYKAEV
                     DLGSEKRIVVVGAAMYYKPEELEEKLVIVCTNLSSRIIGNIESKGMILALDGTEGKPI
                     FLTINKEEKVELGSKVR"
     gene            2663..3310
                     /locus_tag="VF222_03725"
     CDS             2663..3310
                     /locus_tag="VF222_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141567.1"
                     /translation="MSLEEKIFDKIFSSGLSGLKKTDLQKDFASVDLDKILNQWMKQN
                     EIIIAKKGSSNYCWHKDSYLQYLLDSDPKFKYTFELFKEVQISFQNLSASIDKYVEKL
                     DRKMIVLMDSILATTTNNNTFENNITRPQKVINLQSFNEDFDLTICKYSDSIGWVPLS
                     SLRNDLSQKYEISEKEFYDLAEQLVNENRSKYELSTGGNEGIMIRGLVYGYVRCL"
     gene            3310..4920
                     /locus_tag="VF222_03730"
     CDS             3310..4920
                     /locus_tag="VF222_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141568.1"
                     /translation="MYPYNLNKNPFPSSPTPNLEDALILGGKKHQEARNSIFSCIDDL
                     VKYTETTNNSEKFRIITVIQDVGSGKTHLTLNIKISGINEKSIISYLDISQIYPKNIP
                     SIYQSMINGFQSDYLYNLKKELLYYIKDRIKSENKITKKIFKLGFFESLAGRGIDNKI
                     LQILEDRLEPDLQYLENILDDNFSKNDVSIIKTIIQNQFENSKSITLMDMINRLSTLS
                     KLNLKFFNKISLYQFDEFDNDEETLSFMKAIINAHIPNTILMLILTPSSYLKIASKNS
                     SVYDRLEKANYKIDLAGSNSFEEISDIVLEYINFYKLNTAFTDSQRNELLSQIKILYE
                     DFDLRNIRSILNIMYSAFEIASNKKMSIINEQIMDETLRKTYPGLKIKGSIVDISISD
                     YIKIKNRIDSTMDIKIEIVNSLRELINYLHQHNIKCKYEYDLPLEEAINVIYKDYNGS
                     ISQITMLLNESSLQKMTGTMCNNIETTAKNFEYPNIITTDNKSVSINLDRSKIIDLLF
                     VCDKINHNLHTKEDNNKIFLLAKSINLY"
     gene            4984..5445
                     /locus_tag="VF222_03735"
     CDS             4984..5445
                     /locus_tag="VF222_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141569.1"
                     /translation="MKFRQFTTLNDLISNIHEEGFGVIEYERNKVPCIYVQQSLYDII
                     IERCIGKKYVVEVLLNIFYDGYHVFVDIQLEFIGLGIEEYFLIQGNNSINFFDALNDS
                     GLLGIIPKNNLHISASNIFLIQLPKKERISKAVDIIKSNLKYSRDNNEKTN"
     gene            5429..5650
                     /locus_tag="VF222_03740"
     CDS             5429..5650
                     /locus_tag="VF222_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141570.1"
                     /translation="MKKQTNAKKEHSDDVNLQGNNDSVNSSPIPKEIDTHYKLYGKHI
                     WDVKFYEKCEICNSPFDEFGFCACGSART"
     gene            complement(5674..5748)
                     /locus_tag="VF222_03745"
     tRNA            complement(5674..5748)
                     /locus_tag="VF222_03745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5710..5712),aa:Gly,seq:gcc)
     gene            complement(6446..7069)
                     /locus_tag="VF222_03750"
     CDS             complement(6446..7069)
                     /locus_tag="VF222_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141571.1"
                     /translation="MSIKNINSGKENIKNYQNTCSEFIKDLSLDYLHWVDRYNLNQGE
                     TKMRKEKINEIVIKSNHIIYKFGDTIKKIDIIMNDGISKMAEYTAGYPFHFSIVVNNL
                     TSYTYHPIGNIRLNIKAIPNYGKGVRIGNYDKNNLILLNSSSPVNYSVNKSSLESADI
                     LDDYIIIDAKNCQKNDIISATVIVEEVKDNIVFEKRGFSSLVLVIQN"
     gene            7138..7605
                     /locus_tag="VF222_03755"
     CDS             7138..7605
                     /locus_tag="VF222_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141572.1"
                     /translation="MFSQIIEDLKINFNSNRNFFDKYLKRDPNLVYSKINEIAQFVGS
                     RYEIDLKLHFPNPTKIYVFDSYGTENIGIVVDRLKKNFSLSRELIKSKAIETIPDATA
                     SDAYMYEGKEGVRVLFKKGRIELLPGSIHIWCTVDKDIENYVNWILTNVLVKH"
     gene            complement(7625..8326)
                     /locus_tag="VF222_03760"
     CDS             complement(7625..8326)
                     /locus_tag="VF222_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141573.1"
                     /translation="MSSKKLTILVFGSLAVLSIIVNIVIIALNGGFDNPVRSLQIPNA
                     QDLWSAGIKIQDGDEFLYNLTIENRNNNFDNYLIKILFRDSQNYWNTEFTISNESKST
                     KFESLLSKSNLLMRDKQLKNQKYIDIVESSILQIKDITREPKYLIIGAKWDSINTGIT
                     NIPIKITSKQYLFSKVGKLETFVLSYKIEEHNSKIWISKELPLPVKAVIYNVDGKLQY
                     KYDILSTNREIKYKI"
     gene            complement(8397..9461)
                     /locus_tag="VF222_03765"
     CDS             complement(8397..9461)
                     /locus_tag="VF222_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141574.1"
                     /translation="MKKDRLPKLQIFIISVLVSSAILATTGLKFSHAFAVDDNWYVGE
                     GVTKDMYVKYKMSHFDTNNGREFTMTIYFKEQDDKGNWIAPVFVEDQGKVLNGTFILS
                     PLDLTALGTSKIPPEMTKYRSAYAGSLQWLAAFVPKPGLSLTAGSWGKIASIGGSEIK
                     PSGHQELKIPAFKDPIDTVRVEYYKGVASNTYILNEFPYPVMAKTYVDVTTGSAPIQF
                     QYILVETGKGEPAIPEASIVEVTPPLKERTARGDYYVSLDWKPPIITAGTETTFTVDI
                     ADKDQFPVTQASYDFMIMGSNNTILLDLKNQVAREGTKSHSITFEQPGIATIKIKVTS
                     VKGIDTGIFTEQVEFQVPVK"
     gene            9555..9719
                     /locus_tag="VF222_03770"
     CDS             9555..9719
                     /locus_tag="VF222_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141575.1"
                     /translation="MCNCPSVHFYEVEFKLAGMTVVPTHKNCGDSLNEEQLSKFNKEL
                     IKHWRFEESE"
     gene            9800..10054
                     /locus_tag="VF222_03775"
     CDS             9800..10054
                     /locus_tag="VF222_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141576.1"
                     /translation="MAKMLNVNIETNGIDQQEAQDWVTELSNVYADMEIEDIKVSGNN
                     ISFKAGFSGMEDTESDDVKMKIEEYLSMNEAFEVKNISIK"
     gene            10168..10410
                     /locus_tag="VF222_03780"
     CDS             10168..10410
                     /locus_tag="VF222_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141577.1"
                     /translation="MTCKGICVRHKAQKPVGSGRYASGQKRCQICEIFIKWDGLWCPC
                     CGYRLRTKPRNLKYKAKLRARTKKIIEPLTAPVITA"
     gene            10551..10626
                     /locus_tag="VF222_03785"
     tRNA            10551..10626
                     /locus_tag="VF222_03785"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10587..10589,aa:Glu,seq:ctc)
     gene            complement(10770..11357)
                     /gene="endA"
                     /locus_tag="VF222_03790"
     CDS             complement(10770..11357)
                     /gene="endA"
                     /locus_tag="VF222_03790"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HEX7141578.1"
                     /translation="MSKDNETEFAFVIEGVITRNGVVVIESVKQQDQLRSKGYGEKIQ
                     DMYILESYEALFLLYSKRLFLKKKKTIVDFSNFLQTLLKKDLSIFTKFLIFRDLRTRG
                     YIAKEGFGFGNDFRVYERGEYSRKPAKYVIFGINEGISIKANLLYENIRSIEKMGKEA
                     IIAVIERRGEIIYYKVNLKKFTNNIKLLQDKNIEK"
     gene            11431..11925
                     /locus_tag="VF222_03795"
     CDS             11431..11925
                     /locus_tag="VF222_03795"
                     /inference="COORDINATES: protein motif:HMM:NF003239.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7141579.1"
                     /translation="MKGANYREPRGMPYVRREYIAGKPQSKIAKFSSGQASNNYDFKL
                     ELVVTENIQIRHNALEAARLSANKRMAQAGETTFFSKLKVYPHVLLRENKMIATAGAD
                     RLQEGMRRAFGKATGLAARLKPGQTIIEAYVLSGNLSLAKEGFKIASSKIGCPTEIRV
                     TSLK"
     gene            12380..13564
                     /locus_tag="VF222_03800"
     CDS             12380..13564
                     /locus_tag="VF222_03800"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7141580.1"
                     /translation="MKFLQDLEDKEYEGKTALVRVDFNVSIKNGHIGEDYRVRMSLPT
                     IEYLTKRNCKVILASHLGRPKNHQTNYSLKPVAHRLSEILSKTEVRFVNNSIGIEVEN
                     KIKNLSCNGVLLLENLRFYKEEEQNDPEFSKKLASLADIYINDAFSTSHRKHSSTYGA
                     ATLFNIKLGGINLKKELEYLTMIKENPVRPFIIVIGGVKIKDKIGALENLLPKADKVL
                     IGGAAAYTFLKSKGLKIGNSIIDYDHLKWVDHSLEKYGEKIFLPTDHLVSSTPENTSP
                     IYCKEDIPDGMTGFDIGTDTIQNFSTQLGSTRKGTVFWNGPMGLFEKDVFSNGTIHIA
                     KSMALAYMRGSTTLIGGGDTMEGMKKAGVSENEVSHVSTGGGASLRYLAGDKMPGLFI
                     LD"
     gene            complement(13667..14038)
                     /locus_tag="VF222_03805"
     CDS             complement(13667..14038)
                     /locus_tag="VF222_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141581.1"
                     /translation="MRKRKKNSIHNSQITLDEYLIKKFKEKIEKVLLISNKNNHPIVL
                     YRRTIEENEVSIEEEIASVSEKYVVVQIFTHGGYLPVLFREQHIFTPEEFPYWIIKRS
                     RNMVLHCLENIDEFINDYHNG"
     gene            complement(14209..14562)
                     /gene="pth2"
                     /locus_tag="VF222_03810"
     CDS             complement(14209..14562)
                     /gene="pth2"
                     /locus_tag="VF222_03810"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HEX7141582.1"
                     /translation="MELKQVIIVRKDLKMGSGKVAAQVAHAAILSSEKARNKNIEWFR
                     GWFNLGQPKIILKVNSLEELEKINKLGQFNNLPTAIVRDAGLTQLEAGTATCVGIGPA
                     PDYLIDKITGGLKLL"
     gene            14614..15777
                     /locus_tag="VF222_03815"
     CDS             14614..15777
                     /locus_tag="VF222_03815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7141583.1"
                     /translation="MEKKFDSIVVGGSISGLLCAREISKSGLSVAVLEEDYEVGTPEH
                     CGGLVSLDGLKKLGIIPSSKAILNEVNKAKIVSSTKGFEVGTNREKIIVLDRRELDKQ
                     VAFQAQKLGSEIRTRSLVMSVNYDSSKKEFIIKTKDNIFYSQFFIDARGITSLINNHN
                     REGVLQSAQYEVYADWINPDEILVKFDKEKYPDFFAWIIPSGNGKGKFGVAGRKINPL
                     KTVESFIENFGSKYSIIRKIYAPVWIGGPLKKFVFDNTVIVGDAAGQTKPTTAGGIFS
                     CGMGGILAGNHIARYKTKVVNSLLNDYEKDWFSIFGKEFQRMLFFRKIMERLDNKSID
                     AIFSIISDSDILDISKSANFDFHSFALSKILGSKKAIKLISTLLGNEFRNLLH"
     gene            15817..15993
                     /locus_tag="VF222_03820"
     CDS             15817..15993
                     /locus_tag="VF222_03820"
                     /inference="COORDINATES: protein motif:HMM:NF011481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX7141584.1"
                     /translation="MSTLQLPVCSSCNRPIMPNDECVKFYCPNCGYVLLWRCQSCREF
                     ARLYKCVNCSFEGP"
     gene            15995..16276
                     /locus_tag="VF222_03825"
     CDS             15995..16276
                     /locus_tag="VF222_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HEX7141585.1"
                     /translation="MSRLVARIKILPLEAETDLDEVITKLKSNIPTGMGIERYGKEPI
                     AFGINSLICDFSLNDEEGQMDSLEEYIKKTEGVSEIQVINISRKSVNIK"
     gene            16338..16547
                     /locus_tag="VF222_03830"
     CDS             16338..16547
                     /locus_tag="VF222_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141586.1"
                     /translation="MRYPIRNIVYEKIKQSTTLTDSELSSVLSKEGIDLDLSEINKIL
                     LDLEIYGLLKVSWISKDKRRIELIK"
     gene            complement(16578..17465)
                     /gene="cyoE"
                     /locus_tag="VF222_03835"
     CDS             complement(16578..17465)
                     /gene="cyoE"
                     /locus_tag="VF222_03835"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7141587.1"
                     /translation="MDIRSYIEVSKPRIVVVLVITAISSLLGATRFDSTPLIPWDVSL
                     WQVGFLVLAGSLASMGSSALNHYYDRDIDKFMVRTSKRPIPSGKLSAKTVLIYGVSLS
                     ITSVIIAWFTLNPVATFMIALGIFFYVIIYTAWLKRSNAANIVIGGFAGSAASMAGWA
                     TTTGSIDLTGFLVGWLVFMWTPPHFWCLAIKTREEYASVKVPMLPVLIGNQRTARYIF
                     INTAILLPFSISLYFFGLGLLYTAIALISGTLMLIYHYKLVRNPTPEFAWKAYKVTAP
                     YLVIIFIAVAFDALFYFRI"
     gene            17630..18427
                     /locus_tag="VF222_03840"
     CDS             17630..18427
                     /locus_tag="VF222_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7141588.1"
                     /translation="MLTPGIGLTSIGIVGVFVSLSGIAKTFVDGMHAVSLLTLLIGLL
                     FLTPGIFKDGFPSSRKAKLGSFITLGILVIIGLVGAVYSSGQTPTIFVYIGILILISV
                     SAAAVILGIYRNSSKVKIYLASFVVSVIIGIISFSTVGIQLQNPEAESQEKKSETTTN
                     LEPVTNIDITKLATVNILAGSSAQGNPDYDPDQLVVKKANVGIIWINQDNVPHTVTSL
                     KDDGKSFDSSIIMGNANYTLLLSDLKEKEYDYFCTIHPYMKAKFQIE"
     gene            18595..19014
                     /locus_tag="VF222_03845"
     CDS             18595..19014
                     /locus_tag="VF222_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX7141589.1"
                     /translation="MNKKFIIIHDSHLKELAKLAICSLPNESCALLLGKRSDTKHVVT
                     YILPMNNSSHSSIAFNIDPDDLYQAYNKARLMKLDIISIFHSHPSTPIPSDTDKIFML
                     LNPVIWIIYSTSLHTFKAFIYDNLDQLREISIHLIKD"
     gene            complement(19004..20296)
                     /gene="aspS"
                     /locus_tag="VF222_03850"
     CDS             complement(19004..20296)
                     /gene="aspS"
                     /locus_tag="VF222_03850"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX7141590.1"
                     /translation="MKEKTKRTHFAKELKEDIIGQKVNVGGWIEDVRDIGKIIFLVIK
                     DITGPVQVIVDPEKFPQVRDIPRQSSIIVSGTIQRSKAKEMKVELKLEEIGEIYYSKH
                     PLPIDPTGRLESAIDKRLDARALDLRNEKVSRLFAIRSIALEIVRQTLRERSFLEVNT
                     PKIIGSASEGGANLFEFDYFKNKAYLAQSPQLYKEQLVLALDRVFEIAPYFRAENSNT
                     SRHLTEFYSVDIEGAFLDYCDIMEIMERLIKNVIIGINDSGLIDFKTNVVVKKAIETQ
                     FERLTYQTCIEELKEHGEYVVYGDDLSDSSLRKLGQLHPGFYFITKWPLKLKPFYILE
                     NEQDPFLSDSFDLQIGYLELISGGSRQHNPHKIRNRLAEQGLQPDNFSEHLKVFDWGM
                     PPHAGCGLGLDRLIMCLTGSINVREVVLYPRDPYRISP"
     gene            complement(20347..20853)
                     /locus_tag="VF222_03855"
     CDS             complement(20347..20853)
                     /locus_tag="VF222_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141591.1"
                     /translation="MKTPICSFDAKTGILCSKCESKLKTGHLSLGDIEASMKLTGLAD
                     KNNIIDKFALVGAYKIDEELVLILPTSDISILRSNIELLNYIKKEFNNGVWFIESGAT
                     ERRFIENLIFPVKIASINLVWLPDGNKLTRIIISNKKNQNMKIDTNLITKIAKEIRNI
                     ELLVEYIE"
     gene            21031..21780
                     /locus_tag="VF222_03860"
     CDS             21031..21780
                     /locus_tag="VF222_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7141592.1"
                     /translation="MSLELKACESLGIAGESGCGKSTLVSAIMRNISSPGKITQGSIF
                     LDGIEITKLSDKEFDSKIRWKKISIIFQGALNSLDPVYTIKSQIKDIFGIHGQFYDKE
                     KVITILENVGLEKSVLGKYPHELSGGMKQRVVIAIALLLEPDIIIADEPTTALDVLVQ
                     AQIIKVLKKLKEKGISIILISHDLALISALVDKIGIMYAGKIIEFNLTKEIIFSPQNE
                     YTKNLISSTPQLADIYQEKNFKVDDKSSFRF"
     gene            complement(21895..22110)
                     /locus_tag="VF222_03865"
     CDS             complement(21895..22110)
                     /locus_tag="VF222_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141593.1"
                     /translation="MKPLIDYDLDSEYCNFCKGKFNDLSFVTSCEFCGIGIMHDRCAN
                     SHILKSHISELNTKIVQHKDKPLHDFQ"
     gene            22194..>22544
                     /locus_tag="VF222_03870"
     CDS             22194..>22544
                     /locus_tag="VF222_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7141594.1"
                     /translation="MKIYKNPIPTVDAIIQNSSSILLVKRVKDPYKNRFALPGGFVNE
                     GETIEEALVREVYEETSLEVHPIEILGVYSDPTKDPRGHMLTVVFIVLVIRGNPSTGD
                     DINEISWMPINKIND"
BASE COUNT         7981 a         3279 c         3342 g         7942 t
ORIGIN      
        1 ctttacttaa aaatattgaa ttccactaat tttaaaacta gtatgagtga aaataaagga
       61 cctaaaatga ttaaagatga tttttatcct tcattttatt tgaaaaatat gaaaaaggat
      121 ttagatcttg taatggaagc agcgaaagaa aaagaccttt ccttgcctat tacaggtatt
      181 gtttttcaat tatacaatta tgcttcaaga tcaatattct cagatctcga ttatactgga
      241 atatataaat tcttaagaaa acttaatggg ttagaatagg ttaattatgc tgtttataaa
      301 atttaattcg aactctttag cataattttg ttttttgcta tgaaatagta gattccctaa
      361 tttgagtata ttaattttat tttactgaat cctatattct gtttctagtc gaaagatccc
      421 ttttataaaa gaaatgtgtg taaatcaatg tatatataat gataagtcaa ttctcaaaaa
      481 aacgaaattt ttaataactt atccttatat taggaacaga ataaaagtat gtaataattc
      541 cttagagaaa aatgctgaat aatattttta agatcaaacc gttatatata aaattttatt
      601 aagaacggtt gaattatttc gtctatttct atagaaagat aaatcgataa aataattatt
      661 agattctata ggtaggtaat ctgtttactt atttaattcc atgtatgtta ttgttgtttt
      721 tttctgttat aaggaaataa ttggacatat attgtaatat ttgttgatat tattaattaa
      781 gaaaaatttt aaaaggtaga ctgttaaaac taacgaactt tagaacctaa ttcaaccttt
      841 tcctctttat ttattgttaa aaaaattggt tttccctctg ttccatctaa tgctaatatc
      901 attccttttg actctatatt tcctattatt ctagatgaga gatttgtgca tacaataact
      961 aacttttctt ctaattcttc aggtttgtaa tacatagcag ctcctactac tactattctt
     1021 ttctccgatc caagatctac ctctgctttg taaactttgt tcaactttgg tatataatca
     1081 atctttttta tctttccaag tcttaagcta actttactga attcttcaaa tgatatattc
     1141 tcagattcat ctttaatatc actttccatt ttaattagtt aggtatttaa atttccttct
     1201 atataacaca tcgtaataaa acattattat attttttggt tacatataat agtcctttca
     1261 taaatgtatg gtttcaatag atggataaga taataaaaat tggataggtg aagcaataac
     1321 aacaagaaat agtttcaaat ctggtaaagt gtataaatag aaattaaaat atgtttaata
     1381 aataaatgtc ataaaaatat tataaatatc taagtgacaa tgataaaact ctactctcaa
     1441 cgaaacacta tgaaacataa attactcttt taactgtttt tagacacaaa aataaaagat
     1501 aataccatcc actaattgta tagatttttt taggtaaaga ttattatcat gatcttctgc
     1561 ccttttattt taacactagc atagattatc catttttgga aaattctttg tttcatccaa
     1621 tgtttttcca aaataagcca gtatggttag tatggactgt aacaatataa tcaaaatgat
     1681 agaaaagaca tttattctta ctaactcaaa attttaacaa ataaatagta gaattgatta
     1741 tggaaatgat aataatatag ataccaattc tttatacgag aacagatata gatagattaa
     1801 tatattctat aatggtagaa aatattaata accaagaaac agatcataat tctagtttag
     1861 ttgttaaagt ataaacccat ataagaatat tcattgtaat aacgatgtga aataaactaa
     1921 aaaaaatatt gcttactttt agtatgagca ttttagaaaa caatccttta ataattagga
     1981 taaacgtgaa aaggttttaa ttgaatatat aatatgttct aaaatacgac ataaaaaatc
     2041 gtctaaacgt gttgttaaag tgtttttata tttctttgta tttccttaaa tttgttgatt
     2101 tttcaattat ttgagagttg ttttcatggc tagatgtaag aaactcaatt ggagctattc
     2161 ttaccgagga tatcttattt ataataatca atggttatga tcagagttga aacaaacacc
     2221 atccactgtt attgtttaaa tgtactcttt gtatttgtat tttcttgatg atcttagcct
     2281 atgaaatccc ccaaggcatt agtgttagtt aaagatgtga ataatagaaa gtatattttt
     2341 ggggggaatt ggattcatag ttttgtttaa taatctgatt gacaaaaaga aaaagaaaga
     2401 gtatcagatt acactattga tacaactatt agtaaaatgg gtagccaaca aacacttatg
     2461 gttatgaatt gtcccctaca tagtatatga actacaccat atattcggtc agatacccta
     2521 gtgctttcat catatttcag aagagtattt catcattcca tttatcttta aatgactcta
     2581 tattaaaagt taaactaaat tagctagtta tattatataa acatacaatg gatcttttat
     2641 aagaaaacca aaattgtacc ctatgtcttt agaagaaaaa atatttgaca aaattttttc
     2701 ttcaggttta tctggattga aaaaaactga tctccaaaaa gattttgcat cagttgatct
     2761 ggataaaatc ttgaatcaat ggatgaaaca aaatgaaatt attatagcta aaaaaggaag
     2821 ttcaaattat tgttggcata aagatagtta tttacaatat ctattagatt ccgatccaaa
     2881 attcaaatat acttttgaat tattcaaaga ggttcaaatt tcttttcaaa atttatcggc
     2941 ttctattgat aaatatgtag aaaaattgga taggaagatg attgttttaa tggattccat
     3001 cttagccaca actaccaata ataatacatt tgaaaataat attacgcgtc cccagaaagt
     3061 tattaattta caaagcttta atgaagattt tgatttaaca atatgcaaat actctgattc
     3121 catcggatgg gttccattat catctctgag aaatgatcta tctcagaaat atgagatttc
     3181 agagaaggaa ttctatgacc ttgctgaaca gttagttaat gaaaatagat ccaaatacga
     3241 attgtctact ggaggtaatg aaggaattat gattcgtgga ttggtatatg gatatgtaag
     3301 gtgtttgtaa tgtatccata taaccttaac aaaaatcctt ttccaagtag tccaacacct
     3361 aatctagaag atgctctcat tttaggtggt aagaagcatc aagaggctcg aaactcaatt
     3421 ttttcatgta ttgatgatct tgtaaaatat actgaaacca ccaataatag tgaaaaattc
     3481 aggataatta cagttattca agatgtaggt tcaggaaaaa cacatctgac gcttaatata
     3541 aaaatatctg gaataaatga aaaatcgatc atttcttatc ttgacatatc acaaatttac
     3601 ccaaaaaata ttcccagtat ttatcaatcc atgattaacg gttttcaatc tgattacctg
     3661 tacaatttga aaaaagaatt actttattat ataaaagatc gaatcaaatc agaaaataaa
     3721 attacaaaaa aaatatttaa attaggattt tttgaatctt tagccggtag aggaattgat
     3781 aataaaattc tacagatctt agaagaccga ttagaacctg atttacaata tcttgaaaat
     3841 attcttgacg acaatttttc taaaaatgac gtatctataa ttaaaactat aatacaaaat
     3901 caatttgaaa atagtaaatc cattacttta atggatatga taaatcgtct ttctacttta
     3961 tcaaaactta atttaaaatt ttttaacaag atctcattgt atcaattcga tgaattcgat
     4021 aacgatgaag aaacactttc atttatgaaa gctattatta atgcacatat cccaaatacg
     4081 atcctaatgc taattctcac tccatcatca tatcttaaga tagcatcaaa gaatagttca
     4141 gtatatgata gattggagaa agctaattac aaaattgatc ttgctggatc aaattctttt
     4201 gaggaaatat ccgatatagt tttagaatat attaatttct ataagcttaa tactgctttt
     4261 actgatagtc aaagaaatga acttttgtcg caaataaaaa tactctatga agatttcgac
     4321 ttgaggaata taagatcaat attgaatatt atgtatagcg cgtttgaaat agccagtaac
     4381 aaaaaaatgt ccataataaa tgaacaaatt atggatgaaa cattacgtaa aacctaccca
     4441 ggtttaaaaa taaagggaag catcgttgat atatcaatat ctgactatat taaaataaaa
     4501 aatcgtattg attccactat ggatataaag atagagattg taaactcatt gcgagaatta
     4561 ataaattatc ttcatcaaca taatataaaa tgtaaatacg aatatgattt gcctttagaa
     4621 gaagcaatca atgtaatcta caaagattat aatggttcaa tatctcaaat cacaatgctt
     4681 ttaaatgaat catcattaca aaaaatgaca ggtacaatgt gtaataatat tgaaacaaca
     4741 gctaaaaatt ttgagtatcc caatattatc actacagata ataaatctgt ttctataaat
     4801 ttggatcgat caaaaattat tgatttactg tttgtttgcg acaagattaa tcataatctt
     4861 catactaaag aggacaacaa taaaatattt ttattagcaa aatcaataaa cctatattga
     4921 ttttttacat tgtaaacata ttatgaatag actaaaatat ctataaaagt atatttatac
     4981 atagtgaaat ttaggcaatt tactactctt aatgacttga ttagcaatat tcatgaagaa
     5041 ggttttggag tgattgaata tgaaaggaat aaagttcctt gtatatatgt tcaacaatct
     5101 ttgtacgata ttattataga aagatgtatt ggaaaaaaat atgtagtcga agttttactt
     5161 aatattttct atgatggtta tcatgtattt gtagacattc agctggaatt tatcggcttg
     5221 ggaattgaag aatatttctt aattcaaggt aataactcaa ttaacttttt tgatgcatta
     5281 aatgatagtg gtttattagg aattattcct aaaaataatt tacatatctc tgcatcaaat
     5341 atttttctaa ttcaacttcc taaaaaagaa cgcatttcca aagctgtaga tataatcaag
     5401 tcaaatttaa aatattcaag ggacaataat gaaaaaacaa actaatgcaa aaaaagaaca
     5461 ttccgacgat gttaatttac aaggaaataa tgatagtgtc aattcttctc ctattcccaa
     5521 ggaaattgat actcattata aattatatgg aaaacatatt tgggatgtta aattctacga
     5581 aaaatgtgaa atctgcaatt ctccttttga tgaatttggt ttctgtgcgt gtggaagtgc
     5641 taggacttaa ccagatgtga tctttataaa aaatgcgacg accgggattt gaacccgggt
     5701 taccagattg gcaatctgat gtcctagacc aaactggacg atcgtcgccc atacgatttc
     5761 aggtacgttg gttatttagc cttttttatt tcatatagtt acggctttga tcaacactgg
     5821 tcatgactat atatttagtt tagagtagga aactcgttga gattacttct tataatgaaa
     5881 gttttatgat cgggattctc ttttggtatc ataacttttg attatttatt tctattttaa
     5941 tataagatga atgtatacat ttttcttcac tgtgagtact caatttcatt tcttataagg
     6001 tgagattgat agatatattc aatttagttg tttcattgaa cattatattc ctaaaataat
     6061 taaatatttt ttactcatta aaagacatat aataaaaata agaatacata tcactagata
     6121 atgttattca attattattt atatttgtta aattatttcg ttgaaataat tattttaaat
     6181 cgaataaaaa gaagtgtttc taaaattaat ctactaaaat taaataagcc taaagtaaat
     6241 tggataagtg attttgatgt ataaatttga ttatttttaa aattatttga actatttaag
     6301 gtaacaataa tatcttcctt ataaagcatt aaaaattttc ttgatattac atttccatca
     6361 acttatgaaa tataaattta tactaaaaat aaaacaaact tataatatag gtatacagta
     6421 ttagtctgaa ggcttatatt ataggtcaat tttgtatcac taaaactaaa cttgaaaatc
     6481 ctctcttttc aaagacaatg ttgtctttaa cctcttctac aattacagta gcggaaataa
     6541 tatcattttt ttgacaattt tttgcatcaa ttattatata atcatctaat atatctgcag
     6601 attctaaact cgacttgttt actgagtaat taacaggtga tgaagagttt aataaaatta
     6661 gattattctt gtcgtaattt cctattctta ctccttttcc ataatttgga atggccttta
     6721 tgtttaatct tatgtttcct ataggatggt atgtatagct tgtcagatta tttacaacaa
     6781 tagaaaagtg aaacggatag cctgctgtat attctgccat cttagagatc ccgtcattca
     6841 taataatatc tatttttttt atagtgtctc caaacttgta aataatatga tttgatttaa
     6901 tgactatctc gttaattttt tcttttctca ttttggtttc cccctgatta agattgtatc
     6961 tatctaccca atgaagataa tccagcgaaa gatcttttat aaattcagaa catgtatttt
     7021 gataattttt tatattttct ttccctgaat taatattttt aatagacata atttttagtt
     7081 atatttttaa aaactatgag agaatataaa tagatataga attctgtgat acttatgatg
     7141 tttagccaaa taatagaaga tttaaaaata aactttaatt caaatagaaa tttctttgat
     7201 aaatatttaa aaagagatcc taatctggtt tactcaaaaa taaacgaaat agcacaattt
     7261 gtaggctcaa gatatgaaat agatttgaag ttacattttc ctaatccaac taaaatatat
     7321 gtatttgatt cttatggaac cgaaaatatt ggaattgtgg tggatcgact taaaaaaaac
     7381 ttttctttat cccgagaact aataaaatca aaagcaatag aaactatacc agatgcaaca
     7441 gcgtcagatg catatatgta tgaaggaaag gaaggagttc gtgtattatt taaaaaggga
     7501 cgaatagaat tattgccggg atctatacat atttggtgta ctgtcgataa agatattgaa
     7561 aactatgtta attggatatt gacgaatgtt ttagttaaac attaaaagaa aattttagag
     7621 aatattatat tttatactta atttctctat ttgtacttag tatatcatat ttatattgta
     7681 gtttgccatc aacattgtaa atcacagctt ttaccggcaa aggcagctcc tttgaaatcc
     7741 atattttgct attatgctct tctattttgt aactaagaac aaaagtttcc aatttgccga
     7801 cttttgagaa caaatattgt tttgaggtaa ttttaatagg tatatttgtt attccagtgt
     7861 taatggaatc ccattttgcg cctattatca aatattttgg ttctcttgtg atatctttta
     7921 tttgtagtat tgatgattct acaatgtcta tatatttttg attttttaat tgcttatctc
     7981 tcattaggag attagatttt gacaacagag actcaaattt tgttgattta ctttcattag
     8041 atatagtaaa ctcagtattc caataatttt ggctatctcg aaatagtatc ttaatcaaat
     8101 agttgtcaaa gttattgttt ctattttcta tcgttaaatt atacaagaat tcgtccccat
     8161 cctgaatttt aattccggct gaccatagat cttgggcatt aggaatttga agagatctaa
     8221 ctggattatc aaatcctcca tttaatgcaa tgataacaat atttactatg atagaaagaa
     8281 ccgctaatga cccaaatact agaatggtaa gtttcttact tgacacactt ttggttcaaa
     8341 atgaaccact gatcttattt aattttactt agaaaaaaaa tggggtggat taatatctat
     8401 ttaactggaa cctgaaattc tacctgttca gtaaagattc ctgtgtctat accttttaca
     8461 ctagtaactt taattttaat agttgctatc cctggctgct caaatgttat actatgagat
     8521 ttcgtaccct cgcgggctac ttggtttttc aaatcaagta atatagtatt gtttgaaccc
     8581 attatcataa aatcgtagct agcttgagta acgggaaatt gatctttgtc agctatgtct
     8641 acagtaaatg tagtttctgt gcctgcagtt attataggtg gcttccaatc caatgaaaca
     8701 tagtaatctc ccctcgcagt tctctctttc aatggcggag tcacttcgac tattgatgct
     8761 tccggtattg caggttcgcc tttaccagtt tctaccaaga tatactgaaa ctggattggt
     8821 gcagaaccag tcgtaacatc aacatatgtt ttcgccatca caggataagg gaattcgttc
     8881 aaaatatatg tattgctggc tacacctttg taatattcaa ctcttacagt atcgataggg
     8941 tccttaaagg ctggaatttt taattcttgg tgtcctgatg gtttaatctc agaaccacca
     9001 atagaagcta ttttgcccca tgatccagct gtcaaagata aaccaggttt aggaacaaat
     9061 gctgctaacc attgtaaaga accggcataa gcacttctat atttagtcat ttcaggggga
     9121 attttagatg ttcctaatgc cgttaaatcc aatggactta gtataaatgt tccatttaat
     9181 acttttcctt gatcctctac aaaaactggt gctatccaat tccctttatc atcttgctcc
     9241 ttgaaatata ttgtcatagt aaattctcgc ccattattag tatcaaaatg actcattttg
     9301 tatttaacat acatatcttt agtaacacct tctccaacat accaattatc atcgacagca
     9361 aatgcgtgtg aaaattttag tccagttgtg gcaagtatag ccgaagaaac caagacagaa
     9421 atgataaaaa tttgcaattt tggtagtcta tcctttttca ttaattctat tactaaaaaa
     9481 ggattgataa atgtttatta tattattatt tttaattaaa ataatataaa tataatatta
     9541 taacatattt cataatgtgt aattgccctt ctgttcattt ttatgaagtt gagtttaaat
     9601 tagcaggaat gacagttgta cctactcata aaaattgtgg agattcgtta aatgaagaac
     9661 aattatctaa attcaataaa gagttaataa aacactggag atttgaagaa tctgaataac
     9721 acgaataaat atattcatag gtttaaaatt taggcaaatt tattaatgtc tttttattat
     9781 caaagttagg tgtctttgaa tggcaaaaat gcttaatgta aatattgaaa caaatggaat
     9841 agatcaacaa gaagctcagg attgggtcac agaattatcg aatgtatatg ctgacatgga
     9901 aattgaggac attaaagttt ctggaaataa tatttctttc aaagctggat tttcgggtat
     9961 ggaagatact gagtctgatg acgtcaaaat gaagattgaa gaatatcttt caatgaatga
    10021 agcttttgaa gtaaaaaata tctcaattaa atagtatatc cctaagattt actttttttt
    10081 taaattggga taagatctta aaattcttta aaaatcataa atgagcgtta ctcaagttta
    10141 aatagtagtt taggaaatat aaatactatg acttgtaaag gtatttgcgt aaggcataaa
    10201 gcacaaaaac ctgtaggatc tgggagatat gctagtggtc aaaagcgttg tcaaatctgt
    10261 gaaatattta taaaatggga tggtttatgg tgtccatgtt gcggttatag attaagaaca
    10321 aaaccacgta atttaaaata taaagctaaa ttacgtgcaa gaacgaaaaa aataatagaa
    10381 ccattaacag ctccagttat cactgcatag ctgggtttta atatataatt tattttactt
    10441 atcttgtctt taagatagtt agaacctaat tctggttaac ctataatcct ttttttcaat
    10501 tactaggaag gaatagagct tttataacga taaaattatc aacttagtga gctccgatgg
    10561 tgtagtccgg ttaagcattt cgccctctca aggcgacgat cccgggttca aatcccggtc
    10621 ggagcacttt tctataaaat tcagaagtcc ggtatctctt cattttacat gtttccatga
    10681 taataagggg actatcaaaa acatcattat tttatagcat tataatttag tgcgttggat
    10741 acataagtat atcttatcag cgaggttggc tatttctcga tgtttttgtc ttgaagtaat
    10801 ttgatattat tagtaaattt cttaagattg actttataat aaattatttc tcctcttctc
    10861 tctattactg ctataattgc ttccttaccc attttttcta ttgaacggat gttttcgtat
    10921 aatagatttg cttttatact aataccttca ttaataccaa aaattacata tttggctggt
    10981 tttctggaat attctcctct ctcataaaca cgaaaatcat tcccaaatcc gaaaccttct
    11041 tttgcaatat agccacgtgt tctaagatct ctgaatatta aaaattttgt aaaaatcgat
    11101 aaatcttttt tcaataatgt ttgaagaaaa ttggagaaat caacaatggt cttttttttt
    11161 tttaaaaata atcttttcga gtataacaga aaaagagctt cataactttc caggatgtac
    11221 atatcttgga ttttttctcc atatccttta cttcttagtt ggtcttgttg tttgaccgat
    11281 tcaataacta ctactccatt cctagtaata acaccttcta taacaaaagc aaattctgtt
    11341 tcattatctt ttgacatcat catcttatcc tccaacgtaa acgaataaat caaagtctat
    11401 gttaagtata aaaagattcg tgatttaaaa atgaaaggtg ctaattatag ggaaccccgt
    11461 ggaatgccat atgttcgaag agaatatatt gctggcaaac ctcaatctaa aattgcaaaa
    11521 ttttcatctg ggcaggctag caataactat gattttaagt tagaattagt agtgacagaa
    11581 aatattcaaa ttagacataa tgcgttagaa gctgctcgat tatcagccaa caaaaggatg
    11641 gcacaagctg gagaaactac attcttttct aaattaaagg tatacccaca tgttcttctg
    11701 cgagaaaata aaatgattgc tacagctggt gccgaccgac ttcaagaagg aatgagaaga
    11761 gcctttggga aagctacagg gttagcagca cgtcttaaac caggtcaaac tattattgaa
    11821 gcctatgttc tctcaggtaa tctttcatta gcaaaagaag gcttcaaaat agcatctagc
    11881 aagataggat gtcctactga aatacgtgta accagtttaa aataatataa ttcattttaa
    11941 atttttacaa ttttttctaa aattcaaatt taagtttttt tggagtgtcg gaataatcga
    12001 tatttttaca ctcaaaatag ttatacaggg tttctcttca tactttctac taaaattgat
    12061 taattacttg taaatatttt acttgcttca taatatattt gcagtaacta atgattgcta
    12121 ttgtcttcta ttttaataaa ttaaccatat tcaaagaaaa gagaatagtt gaataagata
    12181 tgtttatttt gatctaaaat taaaagttta ttacctcata attttcatga gtttatgtaa
    12241 cctgcctata cattttaata gtataatttt tggataaaaa cttgattatt taattggagt
    12301 ttttttttcg tgctatttaa cctcacttta actatcataa cctttattgt atgagatatg
    12361 tttttctaat ataactaaca tgaaatttct ccaggattta gaggataaag aatatgaagg
    12421 aaaaacagcg ctggtaaggg ttgatttcaa tgtaagtata aaaaatggtc atattggaga
    12481 ggattataga gttcgaatgt cccttcccac tatagaatat cttacaaaaa gaaattgcaa
    12541 agttatttta gcatctcact tgggaagacc taaaaaccat caaactaatt attctctaaa
    12601 accggtagcg catagattat cagaaatact ttctaaaacg gaagtaagat ttgtcaataa
    12661 ctctataggc attgaagttg aaaacaaaat taaaaatctc tcttgtaatg gtgttttatt
    12721 attagagaat cttcgttttt ataaagaaga ggaacaaaat gatcctgaat tctcaaagaa
    12781 acttgcctct ctcgcagata tatatattaa tgatgcattt agtacttctc atcgaaaaca
    12841 ttcttcaacc tatggagcag ctacactctt taatattaaa ttaggtggaa ttaatttaaa
    12901 aaaagaactt gaatatttga ctatgataaa ggaaaatcct gtgcgtcctt ttataatagt
    12961 tattggtggt gtgaaaatta aagacaagat aggagcgtta gaaaatttat taccaaaagc
    13021 agacaaagtc ttgataggtg gtgctgctgc ctatactttt ttaaaatcaa agggattaaa
    13081 aattggaaac tccattattg attatgacca cctgaaatgg gttgatcact cccttgaaaa
    13141 atatggtgaa aaaatttttt tacctacgga tcatcttgta tcatccaccc cagaaaatac
    13201 ttctcctatt tactgtaaag aagacatacc agatggcatg actggttttg atataggaac
    13261 cgatactata cagaattttt ctacacaact tggttctacc agaaaaggga ccgttttttg
    13321 gaatggacca atgggcttat ttgaaaagga tgttttttct aatggaacta tacatattgc
    13381 taaaagtatg gctctcgcat acatgagagg ctcgacaaca cttattggtg gaggggatac
    13441 aatggaggga atgaaaaaag caggtgtgtc tgaaaatgag gtaagtcatg tatctactgg
    13501 gggaggtgct tcattaagat atttagctgg cgataagatg ccgggattgt tcattttaga
    13561 ttgataattt gtaaaactca gaaaactcaa ttttagtata gaaaagcaat tatggattat
    13621 acctagtctt gattaaagaa acctagtctt gattaaagga aataaattac ccattgtgat
    13681 aatcattaat aaactcatca atattttcca aacaatgtaa taccatatta cgactcctct
    13741 taataatcca ataaggaaat tcttcaggag taaagatatg ttgttctcta aatagaacag
    13801 gtagatatcc accgtgagta aaaatttgaa caactacata tttttctgaa acactagcta
    13861 tttcttcctc aatacttact tcattctctt cgatagttct tcgatacaaa acaatagggt
    13921 gattattctt attgctaatt agaagtactt tttctatctt ttctttaaat ttttttatta
    13981 aatactcatc taaagtaatc tgggaattat gaattgaatt tttttttctt ttgcgcaaat
    14041 agtattaaat ataatgtagg actgaaaata aatatttgtg ttaataattt ttaactaaat
    14101 aataagcagt cgattttcat atttattgtt ccaatagtga atataatatt aatctattat
    14161 agttgcatat attttaactt tagttatcta cagttgcaaa atgatttgtt aaagtagttt
    14221 taatcctcct gtaattttgt ctattaaata atctggagca ggaccaattc ctacacaagt
    14281 agcagtccca gcttcaagtt gggttaatcc agcatctcta acaatagcgg tcggtaagtt
    14341 attaaattgt cctaacttat taattttctc taattcctct aatgaattaa cttttaaaat
    14401 tattttgggt tgtcctaggt taaaccatcc cctaaaccat tcaatatttt tatttctagc
    14461 tttttcacta gataatattg cagcatgggc cacttgtgca gctactttgc cacttcccat
    14521 tttcaaatct tttctcacta ttattacttg ttttaattcc attccattaa aaatacattt
    14581 taattattta tattaaaata attttataat atcatggaaa agaaatttga ttccattgtt
    14641 gtaggtggta gcatttctgg attattatgc gcgagagaaa tttcaaagtc tggtctttct
    14701 gttgcagtac tagaagaaga ttatgaagtc ggtactccag aacattgtgg tggtttagtt
    14761 agtcttgatg gattaaaaaa attaggaata atcccatcaa gtaaggcaat tttaaatgag
    14821 gtaaataaag caaagattgt ctcatctaca aaaggatttg aagtcggtac aaatagggaa
    14881 aagataatag tgttagacag aagagaactt gacaaacaag ttgcatttca ggctcagaaa
    14941 ttaggtagtg aaataagaac gagaagccta gtaatgtcgg tgaattatga tagctcaaaa
    15001 aaagagttca taataaaaac caaggataat attttctatt ctcagttttt tattgatgcc
    15061 aggggtatca cttctctaat caataatcac aatagagaag gtgtcttgca atcagcacaa
    15121 tatgaggttt atgccgattg gataaatcca gatgaaatat tagtaaaatt tgataaagaa
    15181 aaatatcccg atttctttgc atggataatt ccgagtggaa atggcaaagg aaagtttgga
    15241 gtagccggga gaaaaataaa cccgttaaaa accgtggaat cgtttattga aaactttggt
    15301 tctaaatatt ctatcatacg gaaaatttat gcaccagttt ggataggagg gccattaaaa
    15361 aaatttgtat ttgataatac agtaatagtt ggagatgcag caggccaaac aaaacctaca
    15421 actgctggtg gtattttttc atgtggaatg ggtggaattt tggctggaaa tcatattgca
    15481 cggtataaaa caaaggtcgt taattcgtta ttgaatgatt acgagaaaga ttggttttca
    15541 atatttggta aagagttcca aagaatgctt ttttttagaa agataatgga aagattagac
    15601 aacaagtcaa ttgatgcgat tttttctatc atttctgatt cagatatatt agatatttca
    15661 aagtctgcaa atttcgattt tcatagcttt gctctatcaa agatattggg atctaaaaaa
    15721 gccattaaat tgataagtac attgttagga aatgaattta ggaatcttct acattaagca
    15781 agatataaat tcggttattt ttcctataga tttaaaatgt caacattgca actgcctgtt
    15841 tgttcatcat gtaatagacc tattatgcca aacgatgaat gtgtaaaatt ttattgtcct
    15901 aattgtggtt atgtactact atggagatgt caaagttgta gagaattcgc acgactatat
    15961 aaatgcgtta actgtagttt tgaaggtcca taaaatgtca cgactagttg ctcgaattaa
    16021 gattttgcca ttggaagcag aaacagatct tgatgaagta attaccaaac taaagtccaa
    16081 tatacccact ggaatgggaa tagaaagata tggaaaagaa cctattgctt ttggaattaa
    16141 ttctttgatt tgtgatttta gtttgaacga cgaagagggc caaatggatt ctctggaaga
    16201 atacattaag aaaactgaag gtgttagcga aattcaagtg atcaatataa gtagaaaatc
    16261 agtaaatatc aagtaaccaa ttattctcac tttaaagata ttttaatata attgctaaag
    16321 cctcttttaa ttagtttatg agatacccca taagaaatat tgtttatgaa aaaataaaac
    16381 aatccaccac cttaactgat tcagaattaa gttccgtcct ctccaaagaa gggattgatc
    16441 tggatctctc tgaaataaat aagatattat tggacctcga aatatatggg cttttgaaag
    16501 tctcatggat ttctaaagat aagagaagga tagagttgat aaaataatga attgtatagg
    16561 cgtgactatg actaatatta aatcctaaag tagaaaagag catcaaatgc aactgcaata
    16621 aatattataa caagatatgg agcagtcact ttgtatgctt tccatgcaaa ttcaggagta
    16681 ggattccgaa caagcttata atgataaata agcatgagag tacctgaaat taaggctatt
    16741 gcagtatata aaagacctaa tccaaaaaag tataaggaaa tggaaaatgg gagcaatata
    16801 gcggtattaa tgaagatata tctggcagtt ctttgattac ctattaaaac aggtaacata
    16861 ggaactttga cacttgcata ctcttctctt gtttttatag caagacacca aaagtgagga
    16921 ggagtccaca taaacacaag ccaacctact aagaacccag ttaaatcaat tgaaccagtt
    16981 gtagtggccc atcctgccat cgaggcagca cttccagcga atcctccaat tacaatatta
    17041 gcggcatttg acctcttaag ccaagcagta taaattataa catagaaaaa tattcctaaa
    17101 gcaatcataa aagtagcaac aggatttaat gtaaaccagg caattattac agacgttata
    17161 ctaagagaga ctccataaat taaaacagtc tttgcagata atttaccaga tggaattggc
    17221 ctttttgatg ttcgtaccat gaatttatct atatccctgt catagtaatg gtttaaggca
    17281 ctagaaccca tagaagctaa tgatccagcc aggactaaaa aaccaacttg ccagagagaa
    17341 acatcccaag gaataagtgg ggtactatca aatctggttg cacctagcaa agagcttatt
    17401 gctgttatta ctaaaactac tacaattcga ggttttgaaa cttcaatata cgatcttata
    17461 tccactgtat tggatctcca aattttattt acatatcttg gatttatttt attcgttata
    17521 catctgttga atgcttatga tttctaaaac tattctaaat ttatcattat tagaaaaggt
    17581 taaaattata gcgaaattct tcaaattata aagcatggca gattgggatt tgttaacgcc
    17641 aggcatagga ttaacttcaa taggaattgt aggggttttc gtctctctat ctggtatagc
    17701 aaaaacattt gttgatggga tgcatgccgt ttcgttgtta actcttttaa tcggcctact
    17761 attcttaact cccggtattt ttaaagatgg atttccctct tctaggaaag ctaaactagg
    17821 atcatttata acattaggaa tattagttat tataggatta gttggagctg tctattcaag
    17881 tggacaaaca ccgacaatat ttgtttacat tgggatacta attcttattt cagtatcagc
    17941 agctgcagta attcttggaa tatatagaaa tagttcgaaa gtgaaaattt atctggcatc
    18001 atttgttgtc tcagttatca taggtattat ttccttttct actgttggaa tccagctcca
    18061 aaatccagaa gctgagtcac aagaaaaaaa atcagagaca acaactaatt tagagcctgt
    18121 aacaaatatc gatatcacaa aattagctac agttaatatt cttgctggct cttcagccca
    18181 aggcaatcca gattatgacc cagatcaatt ggtggttaaa aaagctaatg tgggtattat
    18241 ttggataaat caggataatg tacctcatac agtaactagc ttaaaggatg atggtaaatc
    18301 gtttgattct tctataatta tgggaaatgc taattatact cttttgctct ctgatcttaa
    18361 agaaaaagaa tacgattatt tttgtacaat acatccatac atgaaagcaa aattccaaat
    18421 agaataattt cgttcatcta cttctaacaa gaattaaatt tagtatcatc actttacaat
    18481 taataaggct tattactact atcactaata tttaaatatt aattgcatga ctactgacat
    18541 tatttagttt cctatttttc tatggatata aaggatactt tctttcttaa tttaatgaat
    18601 aagaaattta taataataca tgatagccat ttgaaagagc tggcgaaatt agcaatttgc
    18661 tcattaccta acgaatcttg cgcattattg ttaggtaaaa gatctgatac caaacatgtt
    18721 gttacatata ttttaccaat gaataactct tctcattcgt cgattgcatt taatattgat
    18781 cctgacgatc tctaccaggc atataataaa gctagattaa tgaaattaga tattatttcg
    18841 atttttcatt cgcatccctc tactcctatc ccttccgata ctgacaaaat ttttatgctg
    18901 cttaatcctg ttatttggat aatttactct acttctttac atacctttaa agcatttata
    18961 tatgataacc tcgaccaact tagagaaatt agtatacact taattaagga ctaattctat
    19021 atgggtctct tggatataat acaacttctc ttacattaat tgatccagtt aaacacataa
    19081 ttaatctatc gagtcctaga ccacatccag cgtgaggagg catcccccaa tcaaacacct
    19141 ttaagtgttc tgaaaaatta tcgggttgaa gtccctgttc tgctaatcta tttctgattt
    19201 tatgaggatt atgttgccgt gatccccctg atattaactc tagataacca atttgtaagt
    19261 caaatgaatc agaaagaaag ggatcctgtt cattttcaag aatataaaaa ggttttaact
    19321 ttaaaggcca tttagttata aagtaaaaac caggatgtaa ttgacctaat tttcgtagag
    19381 aggaatctga taaatcgtct ccatatacta catattcacc atgttcttta agttcttcga
    19441 tacaggtctg atatgttagt ctttcaaatt gtgtctcaat tgcctttttt acaacaacat
    19501 ttgttttaaa atcaatcaga cctgaatcat ttatacctat tataacattt tttattaaac
    19561 gttccattat ttccattata tcgcaataat ctaaaaaagc tccttcaatg tctacactat
    19621 aaaattcggt taaatgtctt gaggtattag aattctctgc tcgaaaatat ggtgcaattt
    19681 caaagacacg atccaatgcc aatacaagtt gttctttata aagctgagga ctttgtgcaa
    19741 gatatgcttt attcttaaag taatcaaatt caaataaatt tgcacctcct tcacttgcac
    19801 ttccaattat cttaggcgta ttaacttcaa gaaaagacct ctcccgtaaa gtttgtctta
    19861 caatttctaa agcaatagat cttatggcaa ataatcttga aaccttttca tttcttaagt
    19921 ctaatgctct tgcatctaac ctcttatcaa tcgctgattc taatcgtcca gtgggatcaa
    19981 ttggaagggg atgcttagaa taataaattt ctccaatctc ttctaatttt agctctactt
    20041 tcatttcttt cgctttgctt ctttgtatcg tccctgagac tatgatagag ctttgcctgg
    20101 gtatatcccg gacttgagga aacttctctg gatcaactat gacttgaact ggtcctgtta
    20161 tatctttaat tactaaaaat attatttttc caatgtctcg aacatcttct atccaaccac
    20221 caacatttac tttctgtcca ataatatctt cttttaattc tttagcaaaa tgtgttctct
    20281 tagttttctc tttcaattaa atctatatct aatttggttt atttcatttt ttgcatattt
    20341 atatagttat tcaatatatt caacaagtaa ttctatattt ctaatttctt ttgcaatttt
    20401 agtaatgaga ttggtatcta ttttcatatt ttgatttttc ttattagata ttattatcct
    20461 tgtcagtttg tttccatcag gcagccaaac taaattgatg gaagcaatct tcactggaaa
    20521 tattaaattt tcaataaatc ttctctccgt tgcaccactt tcaataaacc aaacaccatt
    20581 gttaaattct ttttttatat aatttaatag ttcaatattt gaacgtaata tactaatatc
    20641 cgatgtaggg agtattaata ccaattcttc atcaattttg taagcaccaa ctaatgcaaa
    20701 tttatcaatt atattgtttt tatcggctaa tccagttaat ttcatagatg cttcaatatc
    20761 tcccaatgaa agatgaccag tttttaattt agattcacat tttgaacata aaattcctgt
    20821 tttagcatcg aatgaacaga taggagtttt caatatttct agattataat gttattgctc
    20881 cctatatata tagagctaaa acgctataat aattgaaata tatttatcca ggtttaatag
    20941 ttattagatt tgctaaaaaa taagttactc ataaatggtt taagtgtctc ctattttcta
    21001 cataataaat gtattaatgc agttgataat ttgtccctcg aattaaaagc ctgtgaatca
    21061 cttggcattg ctggagaatc aggatgtggt aaaagcacat tggtttctgc aataatgaga
    21121 aatatatcat ctcctgggaa aattacccaa ggaagtattt ttctagatgg aatagaaata
    21181 acaaagttat cggataaaga atttgattca aaaattagat ggaagaagat ttcaattatt
    21241 tttcaaggag ctctgaattc attagatcct gtatatacaa taaaatccca gattaaagat
    21301 atatttggaa tacacggtca attttatgat aaggaaaaag taataacaat tctagagaat
    21361 gttggattag aaaaatctgt acttggaaaa tatcctcatg aattaagtgg tggtatgaaa
    21421 caaagagttg ttatagccat agctttattg ctagaaccgg atattatcat tgctgatgaa
    21481 ccaaccacag cgttagatgt acttgttcag gcacaaataa ttaaggtatt gaaaaagctc
    21541 aaagaaaaag gaattagtat aattttaatt tctcatgatc ttgcattaat ttctgcattg
    21601 gtagacaaaa taggaattat gtatgcagga aaaattatcg agtttaattt aaccaaagaa
    21661 attatatttt ctccacaaaa tgaatatacc aaaaatctaa tatcatctac cccccaacta
    21721 gcagacatct atcaagaaaa aaatttcaaa gtagatgaca aaagttcttt tcgtttctaa
    21781 attggaacat gatattaact atattttaaa atttttctga tagcatcaat atctataagt
    21841 atcatcaaac aaaaaaattt gtggatagct aatatgctaa ctataaaatg aaatttattg
    21901 aaaatcatgt aatggtttat ctttatgttg aacaattttt gtgtttaatt cagatatatg
    21961 actttttaat atatgtgaat tagcacatct gtcatgcata atacctatgc cacaaaattc
    22021 acatgatgtt acgaaagata aatcattaaa ttttccctta caaaagttac aatattcaga
    22081 atcgagatcg taatctatta agggtttcaa ccaaggctct ctccataata actgcatgtc
    22141 taaattacta ggttaaatat atatattatt ttcaattgtc aatgagagaa ctcttgaaaa
    22201 tatataaaaa ccctattcct actgtagatg ctattattca aaactcttct tcgatattat
    22261 tagtcaaacg ggtgaaggat ccctataaaa accgatttgc attacctgga ggttttgtaa
    22321 atgaaggcga gaccattgaa gaggctctcg taagagaagt atatgaagaa acatcattgg
    22381 aggtccatcc aatagagatt ttgggagtat attctgaccc aacaaaagat ccaagaggcc
    22441 acatgctgac tgttgtattt attgtattag ttattagagg aaatccctct acaggggacg
    22501 acatcaatga aatttcctgg atgccaataa ataaaataaa tgat
//
LOCUS       DASVEV010000078         7757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_24338, whole genome shotgun sequence.
ACCESSION   DASVEV010000078 DASVEV010000000
VERSION     DASVEV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24338"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(619..2100)
                     /locus_tag="VF222_03875"
     CDS             complement(619..2100)
                     /locus_tag="VF222_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7141595.1"
                     /translation="MAKEIPSKQRALVLQGGGALGAYQVGVLKVLAKKIIEEDEKNAE
                     QGRPLFDIIAGTSIGAMNAAVLVSNVVRRNKTWSEAICELERFWKVGIALKEGTTSDA
                     DIPPLEMFPMFHWWHPWRKERCDNENNHYTMMGSLASEELARRYYSTKVFVFGGKKVF
                     CAKDVRPDFKFWDENNKRIVLNDLPLQERIKEFGEFPIATSFERNQPRLLITALDIAE
                     GVTVTFDSYKKEDGTMKTTYLSGTKYGYIKNGVHTNNNKSKNNYEADFDPIVIKYEDG
                     IEIEHVMASGTFPEYYDPKIISNRKFWDGGLLSNTPLKEVLESHREYWMNVENKDEAP
                     DLDVYVVNLHPSSIDINNSLSYYDEIKDRNNDIIYGDRTYSEQYSASVIADYAEFIKE
                     LKHLALNHIKEDNDKEAFEKEIENLRTREAENTSYMDGRQKKYEDILRGRFKITKMVR
                     IERKYNPNTSTSFKGGDITSKTIEEMIKEGENDATIEIFHNGS"
     gene            2439..3242
                     /locus_tag="VF222_03880"
     CDS             2439..3242
                     /locus_tag="VF222_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141596.1"
                     /translation="MSDKKIGILSALLFIGVATLFVPVTALQNSIAMAQEYYPEYEEN
                     EYYEKGYDQSKNDDNYKNAPIVNVEKKLFICNNVTDTPNDFECEGFREIFDEGEFNSF
                     PPGPDSGEYIPCNDEICPFIDESDFGVQIFKDVATVRELSPEGTTVNLDKLHYTVTER
                     LIEDRISSDFPCSLVGFTHSLFPEKITTDSIINYFICVNYVGDCEGTIYPGEVKTCTV
                     ENYIYLQDTRPIQIVGNALDDRQSSLLTQQFNNQAIQSQPKMLKDVMSN"
     gene            complement(3861..6068)
                     /locus_tag="VF222_03885"
     CDS             complement(3861..6068)
                     /locus_tag="VF222_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7141597.1"
                     /translation="MISLYFFLCVIFTITVGNLVIIFAEPDSKLIYSNWILIINSLVA
                     AGLASYVFLKSRESINENKENMLLTIGLVFWFIANIIWAYYEVVLDIVSPVPSLADFF
                     LLSAYVFLIYRLMIIRKKLNRIINKKIMYLMLGLTGLFLAYILNLTLDMALTSNFRGT
                     MLFIVTIAYPTLNSILTVLAITILLGIKSEKQHRHLVPWVSESAGFLAIVVGDSWFAI
                     IVLTTSIEQLWISTLLLSAHYLLIAGGLIWYLRYSIKWKSKDLAFKIVARIRKRRLSN
                     KIIVTSITIIGLVLFSSVYLGTGFGGDGGKNSLLIKKNHITEKSSLTSTDSASNGQDN
                     LNKEFVVGAIIPITGSYSSIGKPVKVALEKAEHDVNKYFEKMNSSSRFNLVIANSKSS
                     PEESLEGVKQLHSLGAKIIVGPATSTAVLAAKEYADTNDIILISYSSTSPLLSIAGDN
                     LLRLVPDDINQGKVIAERMIDDGIKVVVPMLRGDIYGNELYKSTKYHYEKLGGKMEEG
                     INYKPHTGKFATSLHRINFIMWDEDLNRLNAIVSDAIKKNDNKSVGVFIISYDEITPI
                     LIQSPMYSTLDDIRWYGSDSIAQNHHITKNIDSALFAIKTNFSNPLYSIDTKSPKIHE
                     LIEILEEELHDGGSITYPAIAYDAFWISSLSLDINSTNSYLIDHKNINKKSFKDIVLT
                     TAESFDNGISGKIQLNDSGDRIGENYDFWGVTKHPQKNQYVWKIENVPIGSSH"
     gene            6219..6452
                     /locus_tag="VF222_03890"
     CDS             6219..6452
                     /locus_tag="VF222_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141598.1"
                     /translation="MSITIHKPLSPSIVNLLHKTNDIVFYSTISFINIKFLIIKAKYY
                     VIEPHSMDGSTLEAGSFLGPTCYRGAYNKKPNI"
     gene            6982..7161
                     /locus_tag="VF222_03895"
     CDS             6982..7161
                     /locus_tag="VF222_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141599.1"
                     /translation="MDELKNQASKIKHEQEKQLGEITKTSLEQKGADDIINTHEKEKR
                     KLEEELSGINLTQII"
BASE COUNT         2638 a         1276 c         1085 g         2758 t
ORIGIN      
        1 cttttctcga ttttaatgat gcttaattgt taggtatatg gatataaaaa gctaaatata
       61 tcattgaatc taagtttgag tatgacagga tcaatcgtct gtagaaccga gcgttcaaat
      121 cccgcccagt ccttttctag agtttattta tttttggtta tgcaatagta atgtagtaat
      181 gaaaaataat tttataaaaa aaatttctat aactaattct aataaattct agtttacttc
      241 aattatttac ataaagtatt tcaaatattg actgaacctg tgtattagta aagttcaact
      301 ccctctgagc ccaatgaatt tgttaattta attgattgat taggattaca ggcaaaaatg
      361 accgttaaac ctctgatata aaagcagaaa tgctacaaac ctatcaagat ttgtatttta
      421 caataaactt aatgttatca atactataga tgcagaaaaa ttaagaaata aattttttaa
      481 atagttaaac ttagtaatga tgaattagta ttacgttact tcgaattcca acaataaaaa
      541 agatatagga ttctagcctt taactcaact ggtctactgt taataaaatc taggaacata
      601 aatcttacaa ttataatatt atgatccatt atgaaaaatt tctatcgtgg catcattttc
      661 accttctttt atcatctcct ctattgtttt tgatgttata tctcctccct taaatgaagt
      721 actggtatta ggattatatt tgcgttcaat tcgtaccatc ttagtgattt taaacctgcc
      781 tcttagaata tcttcatatt ttttctgcct tccatccata taagacgtat tttctgcttc
      841 tcttgttcta aggttctcta tctctttctc aaatgcttct ttgtcattat cttctttgat
      901 atgatttagg gcaagatgtt ttagctcctt gataaattca gcataatctg ctattactga
      961 agctgaatat tgttcggaat aagttctgtc tccatatatt atgtcgttat tcctgtcctt
     1021 gatttcatca taataactaa gtgagttatt aatgtctata ctagatggat gtaagtttac
     1081 tacatataca tctagatctg gggcttcatc tttattttca acattcatcc aatattctct
     1141 atgcgattct aatacttcct tcagtggtgt attgcttaat aagcctccat cccaaaactt
     1201 tcgattagat attattttag gatcatagta ttctgggaat gtaccacttg ccataacatg
     1261 ttctatctcg ataccatctt catatttaat cacaattgga tcaaaatcag cttcgtagtt
     1321 atttttactt ttattattgt ttgtgtgtac tccatttttt atgtaaccat attttgttcc
     1381 tgacaagtat gttgttttca ttgtaccatc ttctttttta taactgtcaa atgtcactgt
     1441 taccccttcg gcaatgtcaa gtgcagttat tagaagtcta ggctggtttc tctcaaaact
     1501 tgtagctatt ggaaattctc caaattcttt tattcgctct tgtaatggta aatcattaag
     1561 tacaatgcgt ttgttgttct catcccaaaa tttaaaatca ggcctcacgt ctttggcgca
     1621 aaaaaccttt ttcccaccaa agacaaaaac tttagtagaa tagtatctcc tagcaagttc
     1681 ttctgacgca agagaaccca tcatcgtgta atgattattt tcattgtcac atctttcctt
     1741 tctccacgga tgccaccagt gaaacatcgg aaacatctcc aatggtggaa tatcggcatc
     1801 agaggtagta ccttctttca aggctatgcc tactttccaa aatctttcaa gctcgcatat
     1861 cgcttcgctc catgttttat ttcttctaac aacattgcta actagaactg ctgcattcat
     1921 tgcaccgatt gaagtgccgg caataatgtc gaataagggt ctaccttgtt cagcattctt
     1981 ctcgtcttct tctatgatct ttttagcaag taccttaagt actcccactt gataagcacc
     2041 tagtgcccca ccaccttgta aaactaatgc tctttgcttg ctaggaatct cttttgccat
     2101 aaaatgttat tatgattttc ttctattata ttttaaagtc atcagtttca ttgggaggtc
     2161 aaagaatata gaagaattaa atattatcta tattctaaaa aacaagatta caggcaaaaa
     2221 tgaccgtcaa acctctgagc ccgaattgat aaccatgatt ttattagggc ctactcctta
     2281 tattgtattc ctttctatta ttaaaataga tataaaaaat actagaaata actaaataaa
     2341 atatttatga tttctctctt ctaatattca atattaatat tagaactgat tgtttctact
     2401 ttactgatat atacatatac taatatgttt tcattatcat gtcagataaa aaaataggga
     2461 ttctcagcgc attgttattc attggagtag caacactgtt tgttccagta acagcacttc
     2521 aaaattctat tgcaatggca caagaatatt atcctgaata tgaagaaaat gaatattatg
     2581 aaaaaggata tgaccaatcc aaaaatgatg ataattataa aaatgctcca atagtcaatg
     2641 tagagaaaaa attattcata tgtaataatg taaccgatac tccaaatgac ttcgaatgcg
     2701 aaggttttcg cgagatattt gatgagggtg aatttaattc atttccccca ggaccagaca
     2761 gtggtgaata tataccatgt aacgatgaaa tatgtccatt tatagatgaa tcagactttg
     2821 gtgttcaaat ttttaaagat gttgcaacag tacgcgaatt atcacctgaa ggtactacag
     2881 taaatcttga caagctccat tatactgtaa ctgaaaggtt aattgaggat agaataagtt
     2941 cagactttcc atgttctctt gttggtttta cacatagttt attccccgaa aaaatcacaa
     3001 ccgatagtat aattaattat tttatatgtg tcaattatgt gggagattgt gagggaacta
     3061 tatatcctgg tgaagtaaag acatgtacag tagaaaacta tatttattta caagatactc
     3121 gtcctataca aattgttggg aatgctttag atgatagaca gtcatcatta ttaacacaac
     3181 agttcaacaa tcaagcaatt caatctcaac ctaaaatgtt aaaagatgta atgtctaatt
     3241 gaagtgatta acaaaaacaa acaattattt ttttttgtat tataaaaaaa ttcttatcca
     3301 agactaatca aaattagatc acctgagaaa gaatattgat catttttatc acaagatagg
     3361 cataaaataa cttctataga atgactttcc tgtatggtga taataccatt cattctttag
     3421 ttgtattgat tatttatcta ttaataataa cggtaatgga tggtgaaaat taagagaata
     3481 aatctttttt aggtcatcat ttaattctac ttttttctgc ttatattcct gttttgatag
     3541 acaattatac ttttgtactg tcagcgtctt cttaataatt aaaatttata ctcttacaat
     3601 tatttgatag acgaatttaa ttcattttta tttacaaatc aaaaaaggga actctaaaat
     3661 tgtaaatgat tatctatttt ttaatagaat tgttcttatg caattttaaa ctataccata
     3721 ttcatttaca ttgttggtcg aaagttatag agtctcatta aacaaatcta gggaaatgat
     3781 atttgtttga gggccccttt tacaagatat acagataaaa acggaaatac aataagtttc
     3841 gataatacat atacatatat tcaatggctt gatcctattg gaacattttc tattttccac
     3901 acatattgat ttttctgagg atgtttagtc acgccccaaa agtcataatt ttcaccaatc
     3961 cgatcccctg agtcattaag ttgtattttt cctgaaatac cattgtcaaa agattcagct
     4021 gttgttaaaa caatatcttt gaaagatttc ttattaatat ttttatggtc aataagataa
     4081 ctattggtac tatttatatc caagcttaat gaggaaatcc agaaagcatc ataagctatt
     4141 gcaggatagg ttatagatcc tccatcgtgt aactcctctt ctagtatttc aattaactca
     4201 tgaattttag gagattttgt atctatggaa tacaaaggat ttgaaaaatt ggttttaatt
     4261 gcaaataatg cagaatctat attctttgta atatgatgat tctgagctat actatcgctt
     4321 ccataccatc tgatgtcatc aagcgtacta tacataggag attgaatcag gataggtgtt
     4381 atttcatcat aagatattat aaaaactcct acagatttat tatcattttt ttttattgcg
     4441 tcagagacta ttgcatttaa tctatttaga tcttcatccc acatgatgaa attaattcga
     4501 tgtaagcttg tagcaaattt acctgtatgt ggtttatagt ttatcccctc ttccattttt
     4561 ccacctagtt tttcataatg atattttgta gatttgtaaa gttcatttcc atagatatcg
     4621 cctctcaaca ttggtacaac tacttttatt ccatcgtcaa tcattcgctc tgcaataacc
     4681 tttccctgat tgatatcatc tggtaccaat cggagaaggt tatctcctgc tatagaaagt
     4741 aatggagaag tactagaata actgataagt ataatatcat tagtatctgc atattctttc
     4801 gcagctaaaa ctgcagtgct tgtagctggc ccaacaataa ttttagctcc aagcgaatgt
     4861 aattgtttta ctccctcaag agattcttct ggacttgatt ttgaatttgc tataacaaga
     4921 ttgaatcttg atgaagaatt cattttttca aaatatttgt ttacgtcatg ctcggctttt
     4981 tccaaagcta cttttactgg ttttccaatt gacgaatagg aaccagtaat tggtatgatt
     5041 gctcccacta caaattcttt atttaagtta tcctgtccat tagaagcaga atcagtagac
     5101 gttaatgatg atttttcagt gatatgattt ttcttaatta acaatgaatt ttttcctccg
     5161 tcacctccaa atccagtgcc aagataaaca gatgaaaaca aaacaagacc aataatagtt
     5221 attgaagtaa caatgatttt gttagaaagt cttctctttc ttattctagc aacaatttta
     5281 aaggcaaggt cttttgattt ccatttaata gaataccgta aataccatat aagaccacca
     5341 gcaatcagta aataatgggc agagagcaac aaagtagaaa tccaaagttg ttctatagat
     5401 gttgtaagaa ctattatcgc aaaccagcta tctcctacta caattgccaa gaaacctgcc
     5461 gattcggata cccatggaac tagatgacga tgttgctttt cacttttaat accaagaaga
     5521 attgttatgg caagaacagt caatattgaa tttaaagttg gataagcaat agtgacaata
     5581 aagagcatcg tacccctaaa attggatgtt agagccatat ccaatgttaa attcaaaata
     5641 tatgctaaga ataatccagt taatcctagc atcaaataca ttattttttt atttataata
     5701 cgattgagtt tttttcttat tatcatcaat ctgtaaatta aaaagacata tgcggacaac
     5761 aagaaaaagt cggcaagaga tggtacagga gatacaatat ccaatactac ttcataatat
     5821 gcccatataa tgttggcaat aaaccaaaaa acaagtccta ttgttaaaag catattttct
     5881 ttattctcat taatactttc tctacttttt aaaaaaacat atgatgctag tcctgcagca
     5941 accagggaat tgataataag aatccagtta gaatagatta gtttactgtc aggttctgca
     6001 aaaattataa ctaaatttcc tacagttata gtaaaaatga cacatagaaa aaaatacagc
     6061 gatatcaatt ctctcttttt ctatatgttt acaatatcga ttataaaatc ttaatactct
     6121 tcaacatgaa ttataattaa taatgacttg agtcttggtg ttgcaattta ttttgttata
     6181 aaatactctt ttatcatact atatgggcca aaaatattgt gtcaataaca attcacaaac
     6241 ctctgagccc atctattgtt aacttattac ataaaacaaa tgatatagtt ttttattcta
     6301 caatatcttt tattaacatt aagtttctaa taataaaagc taaatactat gtaatagaac
     6361 cccactccat ggatggctct actctcgagg ccggtagttt tctcgggccc acctgctatc
     6421 gtggagcata taataaaaaa cctaacattt agaacaaaca agcatatttt gtttaataca
     6481 taaataacat gtgttatatc gataaattag ataaaaatga atttactttc agcattattc
     6541 gatatttgga ttggaaaaac attgttagga tgtttactac atttatttag aaggtaaaaa
     6601 cctatccaac atttttttaa taatggatta attattcctc ataaagaagc ataacgccag
     6661 ttgggtccat agcaatactt atcactggat attgtcgtgt tcacttaaaa attcattaat
     6721 tcttttttca gcttttaatt ttactttcag tttttagttt ggataatttt cctcaaacct
     6781 ttataaaaaa tgaaagctat gaaatgaata ctatgtgtga aggatatgaa attcctgaaa
     6841 aagaagtagt accaaataaa aaacttgaaa aagaaaaaca acctaatcaa gaagaaaagc
     6901 cactaacggt aactgcttaa taagcagtaa actaaccatt tttattgtaa tcgtagttct
     6961 tttggtatag aggttagtat aatggatgaa ctaaaaaatc aagcgtcgaa aatcaaacac
     7021 gaacaagaaa aacagttagg agaaattaca aaaacatcat tggaacagaa gggtgcagat
     7081 gatataataa atacccatga aaaagaaaaa aggaaacttg aagaagaact ctctggtata
     7141 aacttaacgc agattattta ataaaatttc attttttaat tttcaaacat aataagtttc
     7201 taataataaa atctaaatac aagattctgg tatctctaca gtagactctt atttttgcac
     7261 cgctatcggt attactccct ccttgagata tttctataga attcaatact gtaaatattt
     7321 caagaataag ttctaataat aaaagcaaaa tacaatgtag tagaacccta ctacatggag
     7381 ggcactactc ccgacgggcc catagttttc ctcaaatctt ggaattggca ttgtaattgt
     7441 tgtagttgtc atagtctgga cattctacct ataagcccaa ccacttattc aggctattta
     7501 ttagtccaaa gaataatgtc atgaccttct tttttatatc tatatatctt tggctcctac
     7561 ttactagatg gatagataaa ggactattgt aataattaat ttgtctccaa ttattaaata
     7621 ctataaaatc aatttatata atacactccg aaatagcaag actgctaatg tcgtcatagc
     7681 cttcatgttt ttgctttggc tcagaacaat agggctatga ctttttacaa ttattgttat
     7741 taatagtaaa tttgtct
//
LOCUS       DASVEV010000079        13321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1190621, whole genome shotgun sequence.
ACCESSION   DASVEV010000079 DASVEV010000000
VERSION     DASVEV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190621"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(749..1228)
                     /locus_tag="VF222_03900"
     CDS             complement(749..1228)
                     /locus_tag="VF222_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141600.1"
                     /translation="MRISSIKMQNFKVLVVAVFLIIFLFAITSQLSERTLASQMVIGN
                     QSNSSPVDEDTKLGPQDLNTDEEYTITQEMIANDLHDLSSEEIATYPLNELPPADLIM
                     IFNSLSVDDLEKTLKNIHPDNLREILVVKLAQTKVDQILNRLPEYTVREILDRINPS"
     gene            1565..5530
                     /locus_tag="VF222_03905"
     CDS             1565..5530
                     /locus_tag="VF222_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX7141601.1"
                     /translation="MIFLLVVVVLCFSIFAVHDSFPKAVAQMVQQPTTTSLSATNEKQ
                     IIQPWFGFKYLNVTEEIAKAVGLKEAKGRLVTEVITGSPAEKAGVRGGYTLSKIDGQE
                     VKLGGDIILKVDNMDITKYYTAILPNLEKEKKIGDNIQLTLFRNGQIKAINMTFVVIP
                     EFLTYQNSSSGITIQYPNYWEKTQENNVVMFHSPYQSGSDRYQEGLWINIEDLSRQNI
                     TLDEYTNTFSSAYKDFFKDFKFVGSNPTSMTIAGNNTASKLVYTMTSSDSNIHLKFMD
                     IFAIINNKVYHITYAAEVNEFANYLPVIQNIIDSFKLIDLTKDSNSNSLIKSFSSNYS
                     FTPVNSMAEHTLLVYMVGSDLESKSYAATQDILEMEQIGSSSKVNILIETGGGDSQTT
                     IDGKRFIDFTKVQRHKILKNDFQTLSDLGQQNMGDPRILTDFIIWGLSEFPAKRYSII
                     FWDHGSGINGFGKDKRFNDDLLNLDELGKAFKDANTVIYTNFELIGFDACLMASIEVA
                     NSIKSYGNFMVSSEELEPSWGWNYTTILENLTKDPVQNGASFGRTIADSFAKHSRTLA
                     ASQEFGAHKEVTLSVIKLANIPQLIQDLAGLSAYLDNNIVDFPSAMSLAHSVDFTERY
                     GQSSQGGSGLVDIYDLGSNIRERFPQSTLLVDNVHKSLENAILYKVNGDARPNANGLS
                     IYMPLEEDEFSDSSKYSLDSWQKIVDLQYDLIKSDDEIPIVETDIDGETIRGKIYSND
                     VSSIKLIIHHFINEGNRAFYEELEPSSFINNSNASFEYKWKNQVLSLCNSKQICSPTS
                     MDLEVNKDKKFGFIPVVLKSDIRKSEEFATLIYEVNEKAKGFTFLGAVPEIKKYQTIP
                     KEKWALDPKDKIRTYAFAFEDEDPSDSNFAAFPSIQVTDKFEPKYITYNGTFDISFRI
                     CDYSTNCWVTESFRFNGTTEKIKTEPAISSRNNLNISKDEEICPATTNNFSLYENPKF
                     GFKIAYPSTWEKIQKGLLDPWVVAFLYPPGDKWQTVVNIGVENWYNQPSLKEFYESAV
                     KDFPFSKIIESNATTIAHNIGHKIVFTYNSEGQVIQNMFISTVIKGKLYEISFKSNTP
                     KFSDEFSNLIQKMIDSFIINADNREPIAQDQHTICGLKHQQDIKDGTQLNRDTSITTS
                     DFSTYNNPKFGFKIAYPSNWKLGEDLEAHHVIFFTPSETRKNVSALIGDNFVEQIGLT
                     ISPINELPPLNEFVRQYILKIKEDHIGFSLIELNSTTFKENPAYIIVLTLFDPELRIQ
                     IKQMSVLTVIGDNMYRFEYFAESANYEQYLPTIQTMIDSFEIPPDSIAVDSTNVIKQQ
                     R"
     gene            complement(5588..6766)
                     /locus_tag="VF222_03910"
     CDS             complement(5588..6766)
                     /locus_tag="VF222_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141602.1"
                     /translation="MNIKKIVCYSLLFFIILATFSIQAALAQINTSFSLYENHILGIR
                     IQYPSNWEWDQNYDRVVFYLPQDNPGLKFVVSVFPIENRNLDQIINNMPERSWPNFGF
                     IDSPKETTLGVYPGRIMVYTFTEDEHEVKAMRIVTQREDKVYDIVYIAESTQYNEYLH
                     TIDKMISSFEIMLQNNLSTKLDFLTYYNSTYGVTIQYPSSWVKDERGDGSDDCHARVV
                     TFTHPSGYAQLIIFVEYQDDSLYEDYTHKEILEAELEFYRDYDNPDFYDNYKVIESNI
                     NSTLAGYSAYKLEYTQKAGDIEYNSLQIGTEINSDGYFIVADAEAENYSAYFPTIQKM
                     IDSFKMKQPKDFVCLVAGKPPVLAEDTNKTTTEFKAEKPPVLAEDTNKTTSEFQHGIM
                     "
     gene            6804..7130
                     /locus_tag="VF222_03915"
     CDS             6804..7130
                     /locus_tag="VF222_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141603.1"
                     /translation="MIPEKSNPNGNFPTTITDENKGSGLIIDTVQQDPSNRCPDGYHR
                     CPHEDCERVTDTSSMERCPNGFHRNPDRDCERGSDYNIGNEKYYRYYYDDYDDNDIKI
                     IKKSTK"
     gene            complement(7625..8437)
                     /locus_tag="VF222_03920"
     CDS             complement(7625..8437)
                     /locus_tag="VF222_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141604.1"
                     /translation="MKRVRIPFFSKFINLQMDIPVKIDNSQLYVRMDFDRKHDLVYFF
                     ITSKLNKKLDETTISYIDQAYRDYLNNFTALHIWNIKRIFYLLHKLTTSSPIFDFKKM
                     KRIDFVTTTLSSSSLSIQDQITNNIARTIKQNGKIITIINENELKNPSIITLMNFHNL
                     NFQLLNALISIDLVSLLSEKNKKVIKSFIGMGMMIILNLPSLLTLFNCISNEKLFDSF
                     FECFNEGSSDIFYINYILSFLSPILWIVLPKVISYIFSHYFKKMLKKYVSLN"
     gene            complement(8453..8971)
                     /locus_tag="VF222_03925"
     CDS             complement(8453..8971)
                     /locus_tag="VF222_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141605.1"
                     /translation="MSKKNEIPTLEKINLENIFQNFLNLLNNEIKDLRSLEIITVSAE
                     NLLIEIEKHDNNNTTERISLLKDLELFKDEQKVKVLARTHYELDGDIIQFLPVIKNHE
                     NTNSKELKENTADIMSIHNQNVERALKNQQDFIKIMTGFVSQVLIGITGTNGLKGLGN
                     LMDIFVTDKNKT"
     gene            9507..10061
                     /locus_tag="VF222_03930"
     CDS             9507..10061
                     /locus_tag="VF222_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141606.1"
                     /translation="MDSDKQFFEDHNDDLLGSTWTKSDGSFEITFDDLTFSDNWLEKS
                     PEIYILIRNENGQIIHKTDILKIENIDKNKDQIEIPITINLDSLDNKIGLLLNTSSND
                     IYANNSQRILSAFSSLGDVITLNNSNAARNFQLLSSSINAWLIYTNELSWKRIGYDGP
                     QVPKYPLKLPSHSHRLTWEEEETN"
     gene            10058..12757
                     /locus_tag="VF222_03935"
     CDS             10058..12757
                     /locus_tag="VF222_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7141607.1"
                     /translation="MTNCIYGIEYFENRKENYKVINSDVLCDVDVCIIGSGAAGAILG
                     TKLAESGKSVVVLEKGGYYDGESMNQRETDMIPLLWKNAGANFTANLRIAIAQGSCLG
                     GSTVINDAVCFRIPPLVIEQWQKKGVAITKDEWDMANDEVSKRINVQEVTEEELNTNA
                     KKLREACEKFLYQGKSIQKHYKNQRNCGPSISDNSLKSCVKCGFCHLGCHYDTKQSML
                     VTYIHDALVKENINYNVYCNCRADYITYENGIANGVNATFVNSSGLETYRIRVNSKVI
                     IVSAGSIASSNILQKSLIGGEKVGKGLALHPAPFVMGLFDEEIHGNRGIPMSYTCHEF
                     GVTNGIEKGGFLIESIFLPIFQMALAIPSMGLDHKRMMQNYNRYTMAGIMSRDDAVGK
                     VLISYNGNPRVIYNLSEQTIEDMARGMSILSRMWFNIGAKAVVTSHIDAYEIGTKADI
                     PKLKDAVRNNPDGLMLGSAHPQGGNRMGENENECVVDSNCKVFGFDNLYVCDASVFPS
                     ALGVNPQLTVMALATITANKIIQNWKQQDISTNLGNICHLTQPRYCKTEQISEQFAVV
                     DHKENLLQSLVNSDENKIIEDKNWKFNPKTLQIYNNLYWKGFYGRDVDAITTALRYFG
                     GFYKRFEYKNNRIEGITHPFETQVINAKSIATEKQVAGYGKVIHLEYQDFPFSNAYDL
                     LKLVDENTIVGKAFLGQFGRGRELFSFSMSKIYDVDFLTKQDLLTLFHSNELSHKPEE
                     QELVGKWEGMLVSDSSLSPRSQIFYFDYEDGEIDMRYSFANMLHGRSDITITENSIFR
                     LDDQTPFHDEIRMVTPQVAVGLWISEWSSNNILEPIIQDLSRYYPIKIANNDILKDKL
                     LFLKQFYLPGIRLPKELGLSFLQVEENEQKETRIGLSYILKRI"
BASE COUNT         4704 a         1884 c         2141 g         4592 t
ORIGIN      
        1 tccatctccg ccatcatctt gactttgtac aataggtaat atgtcgaatg aaggtaatat
       61 cgttataaca gtagttgcta gcaatattat aaaaagggaa tatgaaatac tagcaaccat
      121 aattagtata tgcaatttaa aaatttaaag ttattcatag aataacaaaa aagtgacttt
      181 ctcaaatcag acgcatataa tatattaaat tctaattgat ggtatctttt aaattattat
      241 ttgttatcaa tatgaatcac tttaaaacta ctgataataa gatataatta aatattttaa
      301 ttcattattc ctctaatttt taatcgccga aaaaatagat taattttatt acattcatca
      361 tttttgttat ccatttttta ttctttttta attgatctct tgcctatttg tacccttcat
      421 tgaacaacag ggttattgtt ttataggaat aatctgcgca tttattcgag atagtattta
      481 catcatcttg acggtcaatt cttgttatat tatctatagc aatttacctt ttagtaaatc
      541 aatatatttt ctcggttctc ctgttcctca atttactttt tgctttgtta tttagtatgg
      601 tactattatg caaatctgcc aatacctttg gtatttcagt attaacacaa cactactgta
      661 gcttattttt cagtatataa aagtggtaaa tattaatcaa tattcaaact tactttatgt
      721 aacctagggt aatttacttt tgttattgtt aggaaggatt aattcgatct aaaatctctc
      781 gaactgtgta ttcaggcagt cgattaagaa tttggtcgac tttggtctga gccaacttta
      841 caaccaatat ttctctaaga ttatctggat gaatattttt caaggtcttt tctagatcat
      901 ctacagaaag actattaaat atcattataa ggtctgctgg tggaagctcg tttagaggat
      961 atgtggcaat ttcctcagag cttaaatcat gcaagtcatt tgctatcatt tcttgagtaa
     1021 tagtgtattc ttcgtcagtg tttagatctt gcggtcctaa tttggtgtct tcgtctacag
     1081 gggagctgtt actttgatta cctatcacca tttgtgatgc tagtgttctc tctgagagtt
     1141 gactagtaat ggcaaacaag aagattataa gaaatacagc tactaccaaa actttgaaat
     1201 tttgcatttt tatacttgag attcgcaata atataaaaac aatctcgtta ctatgcacta
     1261 ttgtgtagat cacaatttat gataattccc attagtatca catcgaactc gtttgtactt
     1321 ctttaaccat ttcaataccc ttatgcatcc aatattttaa taattgtgag atcccaacat
     1381 tatcgtacgt attattatta tacatttaag ttaggaattc taaagatctg tattgcattt
     1441 acaaaaaata agtgtctggg tgaaataatg ttataaacat gtattaacaa agacaataca
     1501 agtgttatta tctaataata gtcttgtaca taaaagccca ctttttttag aatacatcat
     1561 aatcatgatc tttcttcttg tggtagtagt attatgtttt tccatttttg ctgttcatga
     1621 ttcttttcca aaagcagtag ctcagatggt tcagcagccc accactactt cattgtctgc
     1681 aactaatgaa aaacagatta tacaaccttg gttcggattt aagtacctga atgtgacaga
     1741 agaaatagcg aaagcagttg gattaaaaga agccaaggga cgtctagtga ctgaggttat
     1801 tactggaagc cctgctgaaa aagcaggagt acgtggaggt tatacactat ccaaaatcga
     1861 tggacaggag gtgaaattag gaggtgatat aatactaaag gtagacaata tggacattac
     1921 taagtattat actgctatat taccaaatct tgaaaaagaa aagaagattg gtgataacat
     1981 acaactcact ttatttagaa acggtcaaat aaaagcaatt aatatgacat ttgttgtaat
     2041 acctgagttt ttgacatatc aaaattcgtc ttctggaata acaattcaat atccaaatta
     2101 ttgggaaaag acacaagaga acaatgtagt tatgtttcat tctccatatc aaagtggttc
     2161 agataggtac caagaaggac tttggataaa tatagaggat ttgtcccgtc aaaatattac
     2221 tctcgatgaa tataccaata catttagtag tgcatataag gactttttta aagatttcaa
     2281 atttgttgga tcaaatccaa caagtatgac aattgcaggg aataatacag catccaaatt
     2341 ggtatatact atgacatcat cagatagcaa tatccatctt aaattcatgg atatttttgc
     2401 tataattaac aataaagtat atcacattac atatgcagca gaagtaaatg aattcgctaa
     2461 ttatttgcct gttatacaaa acataattga ttcatttaaa ttaattgacc ttacaaaaga
     2521 cagcaactct aattctttga taaaatcatt ttcttctaat tattcattta caccagttaa
     2581 ttctatggct gagcatactc ttttggtata tatggtaggt tcagatttgg aatctaaatc
     2641 atatgcagct acacaagata ttttggagat ggagcaaata ggatcctcat ctaaggtcaa
     2701 tatactaatc gaaacgggtg gcggagattc acagacaaca attgatggca aaagatttat
     2761 tgattttact aaagtacaga gacataaaat actcaagaat gattttcaga ctctatcaga
     2821 tctgggacaa caaaatatgg gtgatcccag gatactcaca gattttataa tatggggact
     2881 atcagaattt cctgcaaaaa gatactctat tatattttgg gatcatggta gtggaataaa
     2941 cggttttggt aaagataagc gttttaatga tgacttgtta aatcttgatg agctaggaaa
     3001 agcgtttaaa gatgcaaaca ctgtgatata tacaaatttt gaattaattg gatttgatgc
     3061 ttgtcttatg gcttctatag aagtcgcaaa tagcataaaa tcatatggga actttatggt
     3121 ttcatctgaa gagttggaac catcatgggg atggaattat actactatac ttgaaaattt
     3181 gactaaagat ccagtgcaga atggtgcttc attcggtaga acaattgctg attcgtttgc
     3241 taagcattct agaactcttg cggcctctca agagtttggt gctcacaaag aagttacact
     3301 atctgtgatc aagttagcta atatccctca actgatacaa gatttggcag gtttatctgc
     3361 atatcttgat aacaatatag ttgacttccc ttctgccatg tctcttgcac attctgtaga
     3421 ttttaccgaa cggtatggtc aaagttcaca aggtggatct gggctagttg atatttatga
     3481 tcttggatca aatattagag aaaggtttcc ccaatcaaca cttttagttg ataatgttca
     3541 taaatcatta gaaaatgcaa tcttatacaa ggtaaatgga gatgcaagac ctaatgccaa
     3601 tggtctttct atttacatgc ctttggaaga agatgaattt agtgattcta gtaaatatag
     3661 cttggatagt tggcaaaaaa ttgttgattt acagtacgat cttataaaat cggatgatga
     3721 gatacctatc gttgagacag atattgatgg agaaaccata agaggaaaaa tatactcaaa
     3781 tgatgtttca agtataaaat tgattataca tcatttcata aatgaaggta acagggcttt
     3841 ttatgaagag ctagagcctt catcgtttat taacaatagc aacgcatcat ttgaatacaa
     3901 atggaaaaat caggttttat ctctatgtaa cagtaagcag atctgttctc ccacttcaat
     3961 ggatcttgaa gttaacaagg ataagaaatt cgggtttatt ccagtagtac tcaaatccga
     4021 tatacgcaag agtgaggaat ttgcgacatt aatctatgag gtaaacgaaa aagcgaaagg
     4081 atttacattt ttaggtgcgg tacctgaaat aaagaaatat caaacgatac caaaagaaaa
     4141 gtgggcttta gatcccaaag acaagattcg tacttatgca tttgcatttg aagatgaaga
     4201 tcctagtgat tccaactttg ccgcatttcc ctcaatacag gttacagata agtttgaacc
     4261 taaatatata acctacaacg gaacgtttga tatcagtttt agaatatgcg attattcaac
     4321 taattgttgg gtaactgaat cattccgctt caatggaact acagaaaaaa taaaaacaga
     4381 accggccata tcttccagaa acaatttgaa tatttcaaaa gacgaagaga tatgtccagc
     4441 cactacaaac aatttctctc tatatgaaaa tcctaaattt ggatttaaaa tagcatatcc
     4501 ttctacgtgg gaaaaaatcc aaaaaggtct tttagatccc tgggttgtag cgtttcttta
     4561 tccgcctgga gataaatggc aaacagttgt taacataggt gtagagaatt ggtataatca
     4621 accatctctg aaagaatttt atgagtctgc cgttaaggac tttccatttt ctaaaattat
     4681 tgaatctaat gcaactacta ttgcacataa tataggacac aaaatagtgt ttacatataa
     4741 tagcgaagga caagtaatcc agaatatgtt tatttcaact gtaattaaag gcaaattata
     4801 tgaaattagc ttcaaatcaa atacaccaaa attctctgat gagttttcta atcttattca
     4861 aaaaatgatt gattcgttta taatcaatgc cgacaataga gaaccaattg ctcaagacca
     4921 acacactata tgtggattaa aacatcagca ggatataaaa gatggaacac aattgaatcg
     4981 ggatacctct atcacaacaa gtgatttctc cacatataat aatcctaaat ttggatttaa
     5041 aatagcatat ccctctaact ggaaattagg tgaagatttg gaggcccacc atgttatatt
     5101 tttcacacct tcagaaacaa gaaaaaatgt ttctgcatta attggagata atttcgttga
     5161 gcagattggg cttactatct ctccaatcaa tgaattacca cctctaaatg aatttgtccg
     5221 tcaatatatt cttaagatta aggaggatca cataggattt agtcttattg aattgaattc
     5281 aactacgttt aaagaaaacc ctgcatatat catagttctt acactctttg atcccgaact
     5341 taggatacaa ataaagcaaa tgtctgttct tactgtaatt ggcgataata tgtatcgatt
     5401 cgagtatttt gcagaatcag caaattatga gcaatatctt ccgactatac aaactatgat
     5461 tgactcattt gagattcctc ctgactcaat tgcagtcgac tcaacaaatg tgatcaagca
     5521 gcagagatag acaagataat agattcgtaa gtggctatta tagagtaata atctaggtcc
     5581 aaatagttca cattatacca tgttgaaatt ctgaagtagt cttatttgtg tcttcggcca
     5641 gtacaggggg tttttcagct ttgaattctg tagtagtctt atttgtgtct tcggccagta
     5701 cagggggttt cccagcaact aaacatacaa aatcctttgg ttgtttcatt ttgaatgaat
     5761 caatcatctt ttgtattgtc ggaaagtaag cagaataatt ttctgcttca gcatcagcta
     5821 caataaaata accatccgaa ttaatttctg ttccgatttg caaactattg tattctatat
     5881 ctccagcttt ttgagtatat tctaatttat aggcagaata gcctgcaagt gtactattta
     5941 tattggactc aatgaccttg taattatcat aaaaatcagg attatcataa tcccgataaa
     6001 actcaagttc tgcttctaaa atttctttat gtgtatagtc ttcataaaga ctgtcatctt
     6061 gatattctac aaatatgata agctgtgcat atcctgaagg atgtgtgaat gtaacaactc
     6121 tagcatgaca atcgtcgcta ccgtctccac gttcatcctt tacccaactt gaagggtact
     6181 gtattgtaac tccataagta gaattgtaat atgtcaagaa atctagtttt gttgataagt
     6241 tattttgcaa cattatttca aatgaactaa tcatcttgtc aatggtgtga agatattcat
     6301 tatattgagt tgattccgcg atataaacaa tatcatacac tttatcttcc ctttgtgtga
     6361 caattcgcat tgctttgact tcatgttcat cttctgtgaa ggtatatacc attatacgtc
     6421 ctggataaac acccagcgta gtttctttag gggagtcaat gaatccaaag tttggccagg
     6481 atcgttcagg catattattg ataatttgat caagatttct attttcaata ggaaaaacgg
     6541 atacaacaaa tttgagacct gggttgtctt gtggcaaata gaagacgact ctatcataat
     6601 tttggtccca ttcccaattg gatggatatt ggattctaat accgaggata tggttttcat
     6661 atagtgagaa gcttgtattt atctgtgcta atgcagcttg tatagagaat gttgctaaaa
     6721 taatgaaaaa tagcagtgaa taacaaacaa tttttttaat attcaattat ttgtgaagta
     6781 atacataccg atgatattta ggtatgattc cagagaagtc caatccaaat ggaaattttc
     6841 ctactacaat aacagatgaa aacaaaggaa gtggtttaat cattgatacc gttcaacaag
     6901 acccaagcaa cagatgtcct gatggctatc atagatgtcc acatgaagat tgtgaaagag
     6961 ttacagatac atcatccatg gaaagatgtc ctaatggctt tcatagaaat ccagatagag
     7021 attgtgaaag aggatctgat tataatatag gcaatgaaaa gtattatcgc tattattacg
     7081 atgattatga tgacaatgat attaagataa tcaaaaaatc taccaaatag taaaatcaaa
     7141 tagataaaaa atatatataa taaaatgact ggagtatcaa gttaaaataa tactatattt
     7201 actttaacaa aaactttatt tattatgtta cctaactctg ttataatgag aatttgaagc
     7261 cctctgagct acaactttag atataaaaaa taattgaact catttagtga gtggaatcaa
     7321 ttagataaaa tcttatttcg aaattttata gatataattt tttagttctg aaaaattgtc
     7381 aatagaagct gacagaaaat attaaaaata agatataatg tgatcataca aacagaataa
     7441 tattatattt tgttagcatt actcttgttg agtttgtttt taatcgttga agggttttgt
     7501 caaactctga tagtaatgct atgtagagaa gttatatacg ttattatcta ctattaacat
     7561 ctttatagca tatgttttta atttctatcc ttctcattat ttttattttc aaatcgaata
     7621 gtagttaatt aagagaaaca tattttttta acattttctt aaaataatga ctaaatatat
     7681 atgaaataac ttttggtaat acaatccaca gaattggaga taaaaagctt aaaatataat
     7741 ttatataaaa aatgtcagaa gatccttcat taaagcattc gaaaaaacta tcaaataatt
     7801 tttcatttga aatacaattg aataaagtaa gtaaactagg taaatttaat attatcatca
     7861 tacccattcc aataaagctt tttattacct ttttattttt ctcactaagc aaagatacta
     7921 aatctatact aattaatgca tttagtagtt gaaaattaag attatgaaaa ttcattaaag
     7981 taataattga tggatttttt aactcattct cattaattat tgttattatt tttccatttt
     8041 gtttgattgt tctagcgata ttatttgtaa tttgatcctg tattgataat gatgatgagg
     8101 ataatgttgt tgtaacaaaa tctattcgtt tcattttttt aaaatcaaaa atcggagaac
     8161 ttgttgtaag tttatgaagc aaataaaaaa ttctttttat attccatatg tgtaaagctg
     8221 taaaattatt caaatagtct ctatatgctt gatctatata ggaaatagtt gtctcgtcca
     8281 attttttgtt tagtttagaa gttataaaaa aataaactaa atcatgcttt ctatcaaaat
     8341 ccattctaac ataaagttga gaattatcta ttttaacagg tatatccatt tgtaaattaa
     8401 taaatttaga aaaaaaaggt attcgaactc ttttcatgac ttttttttat ggttaggttt
     8461 tattcttatc agttacaaag atatccatta aattacctaa accttttagg ccatttgttc
     8521 cagtgattcc tattaatact tgtgaaacaa atccagtcat tatttttata aaatcttgtt
     8581 ggttttttaa tgctctttca acgttttggt tatgtatact cattatatca gcagtatttt
     8641 ctttcagttc tttagaattt gtattctcat gattttttat tacaggtaaa aattgaataa
     8701 tatctccatc taattcgtag tgtgttcggg ctaagacttt caccttttgt tcatctttaa
     8761 atagttctaa gtcttttaag agactaattc gttcagtagt attattatta tcgtgttttt
     8821 ctatttctat taataaattc tcagctgaga cagttattat ttctaaagat ctcagatcct
     8881 ttatttcgtt attgagtaga tttaaaaaat tctgaaatat attttctaaa ttaatctttt
     8941 ccaaagtcgg aatttcgttt tttttactca tttaaagtca atataggcat attaattaat
     9001 aaattattta aaaatgatct tatcggatac tctaacatat atttcaacaa agaataatcc
     9061 tcattatgac ttcctttcgt ttatatatga tatgtccatc aacttgaaca atttttcggc
     9121 gccgcaaaac ttttgaaata gtttgattat ttcaattttc ttgaaaatat tggtttcatt
     9181 ctcttagttc tctagtacct tctccagcaa ttaatagttg ataactattt ttattatcat
     9241 tattgttatt attacttatc ttaataattt gaaatattat tggaaagtat cttttaaagg
     9301 taatcgtaag atgagatatc tttttttaag catatatctt attagataat aatcaaaata
     9361 ttacacaaca aatagttaat cctagtgaat agtcaaaaaa tcctatatat ttggattcta
     9421 tttgtatttg tgtgactatt tactctatta aaggaacgat aattaattta agaactcatc
     9481 taaataattt attaaagatt caagcgatgg acagtgacaa acaatttttt gaagatcata
     9541 atgatgatct attgggatct acttggacga aatctgatgg atcgtttgag attacttttg
     9601 atgatttaac ttttagcgat aattggttag agaaatcccc tgaaatctat atattaataa
     9661 gaaatgaaaa tggacaaata attcataaaa cagatattct taaaattgag aatattgata
     9721 aaaataaaga ccagattgaa ataccaatta caataaatct tgattcctta gataataaaa
     9781 taggattatt attaaatact tcttctaatg acatttatgc caacaatagt cagagaattt
     9841 tatcggcatt ttcttcttta ggtgatgtta taacactaaa taatagcaat gctgcgagga
     9901 actttcaact tttaagtagt tcaatcaatg catggctaat ttacactaac gagcttagtt
     9961 ggaaaagaat tggatatgat ggtcctcagg ttcccaaata tccattgaaa ttacctagtc
    10021 atagccacag attaacttgg gaggaagagg aaactaattg acaaattgta tctatggaat
    10081 agaatacttc gaaaatcgta aagaaaacta taaagtaatc aattctgatg tgttatgcga
    10141 tgtagatgta tgtataattg gttcaggtgc cgctggtgca atattaggaa ctaaacttgc
    10201 agaatcagga aaatctgttg ttgtcctaga aaagggtgga tattatgatg gtgaatcgat
    10261 gaatcaaaga gaaacagata tgattcctct tttgtggaag aatgccggtg caaatttcac
    10321 cgcaaatttg agaattgcta tagcccaagg ttcctgtcta ggtggctcaa cagtaataaa
    10381 tgacgcagtt tgttttagaa tccctccact cgttattgaa caatggcaga aaaaaggagt
    10441 tgcaattaca aaggatgaat gggatatggc aaatgatgaa gtttcaaaga ggataaatgt
    10501 gcaagaagtt actgaagagg aacttaatac taatgcaaaa aaacttagag aagcatgtga
    10561 aaaatttctt tatcaaggaa aatcaattca aaagcattat aaaaatcaga gaaattgtgg
    10621 tccttctata tctgataatt ctcttaaatc atgtgtaaaa tgtggttttt gccatcttgg
    10681 gtgtcattat gatacgaagc aaagtatgct agtaacttat atccatgatg cactagtcaa
    10741 ggaaaatata aattataatg tatattgtaa ttgtcgtgct gattatatca catatgaaaa
    10801 tggaatagcc aatggtgtaa atgcaacatt tgtaaattcg agcggtttgg aaacttatag
    10861 aatacgtgtt aactcaaagg ttattatcgt atctgcaggt tctattgcat cgtctaatat
    10921 tttgcaaaaa tcacttatag gtggtgaaaa ggttggcaaa ggattagcac ttcatcctgc
    10981 accttttgtt atggggcttt ttgacgaaga aattcatggt aatagaggta taccaatgtc
    11041 ttacacttgc catgaatttg gtgtaactaa cggtatagag aaaggaggat ttttaattga
    11101 aagtatattt ttacctatct ttcaaatggc tctagcaata ccgagtatgg gattagatca
    11161 caaaagaatg atgcaaaatt ataatagata taccatggcc ggtataatgt ctagagatga
    11221 tgcagtcggg aaagtcttga tatcttataa tggcaatcca agggtgattt acaatttatc
    11281 tgaacaaaca attgaggata tggccagagg tatgagtata ctatctagaa tgtggtttaa
    11341 tataggagca aaagcagtag ttacatctca tatagatgcc tatgaaatag gaactaaagc
    11401 tgatattcca aaactaaaag acgcagtacg aaataatcct gatgggctta tgcttggttc
    11461 ggcacatcct caaggtggaa ataggatggg agaaaacgaa aatgaatgtg tagttgattc
    11521 taattgcaaa gtgtttggat ttgataacct gtatgtttgt gatgcaagtg tttttcctag
    11581 tgcacttgga gttaatcctc agttaactgt aatggctctt gctactataa cggcaaataa
    11641 aattatacaa aattggaagc agcaagacat ctctacaaat ttgggaaaca tatgtcattt
    11701 aacacagcca aggtattgca aaacagaaca aatttcagag cagtttgctg tagtagatca
    11761 taaggaaaat cttttacaat cattggttaa ttcagatgaa aataaaataa ttgaggacaa
    11821 aaattggaag tttaatccaa agacacttca aatttataat aacctatact ggaagggatt
    11881 ttatggacga gatgtagatg caattactac tgccttaaga tattttggag gcttttataa
    11941 aagatttgaa tataaaaata acagaattga aggaataacc catccttttg aaacacaagt
    12001 aataaatgct aaaagtattg ctactgaaaa acaggtagct ggatatggca aagtaattca
    12061 tcttgaatat caagacttcc ccttttctaa tgcatatgat ttgctaaaat tagttgatga
    12121 aaatacaatt gttgggaaag catttttagg gcagtttgga agaggaagag aattatttag
    12181 ttttagcatg tcaaaaattt atgatgtaga ttttcttaca aaacaagatt tattgacatt
    12241 gttccatagt aatgagctga gccataaacc cgaggaacaa gaacttgttg gaaaatggga
    12301 aggaatgtta gtatctgatt catctctatc accacgttct caaattttct attttgatta
    12361 tgaagatgga gaaattgata tgaggtattc atttgcaaac atgttgcatg gaagatcaga
    12421 tatcacaatt acagaaaata gtatatttag attagatgat caaacaccat ttcatgatga
    12481 gataagaatg gttactccac aagttgcagt tggtttgtgg ataagtgaat ggtcatctaa
    12541 caatatttta gaacctatta tacaagattt gtcaaggtat tatccaatta aaattgcaaa
    12601 taatgacatt ttaaaagaca agttattatt tctaaagcaa ttttatttgc caggaattcg
    12661 attacctaaa gaattagggt taagtttctt gcaagtagag gaaaatgaac aaaaggaaac
    12721 aaggatagga ttatcatata ttctaaaacg aatataaaaa attggattaa aaccttaact
    12781 tgtacgttaa tacataacta taagacttga ttctttatta tcccttattt aggttactta
    12841 cttattattt tatgttttag agaatatcaa attgtcggat tctgttactg tctttcagta
    12901 ggtatttttg gatataaaat taatatttta tttttccaaa tagttataaa gttatttaat
    12961 aaacaggatt tcatacatct taagtgtaaa ttaagaacta tattattaaa attgattcta
    13021 cagtcacttt tggaaacata aggtattaaa tacatataca tttgatcatt tttaaaattt
    13081 tgttataaaa aaaataagat tgatgataat attaattaca tattataata ttataagaaa
    13141 tattttaaaa attatgtatt ttaatactgt attatcatgg aaatggtttt gttaaagtat
    13201 ttgttgctta agaaaataac caaaacttta taaatatatt atagagatac tgaatcaaaa
    13261 aacaaaaatg atttcttata caataaataa ttattaaatg gaattaacta tataataaat
    13321 t
//
LOCUS       DASVEV010000080         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1190702, whole genome shotgun sequence.
ACCESSION   DASVEV010000080 DASVEV010000000
VERSION     DASVEV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190702"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VF222_03940"
     CDS             <1..271
                     /locus_tag="VF222_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141608.1"
                     /translation="AVLLFSAVFSFLFVGHTNGINFAVAQTANTSDPGPETIQDFEKE
                     IGDDNSAIIRNDTNISNPNNTLLDATEVNIEEDCMVLKDGSNYCP"
     gene            complement(463..1314)
                     /locus_tag="VF222_03945"
     CDS             complement(463..1314)
                     /locus_tag="VF222_03945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7141609.1"
                     /translation="MKTKNIKPKSERKVIIQKKSEQKRDVDNVNAKILNRIIGSSTKE
                     NENRKDQLVDLQTLFSQRQDRLWKALEERYQYNSSVKHGQEFLLNHVNSKVELVVMYI
                     DLVGSTKMSMTLPVEQLVTIMRAFSHEISSVVESYNGYVLKYVGDAIISFFPCGFNKY
                     LISDKSVQCAKSMINVIKNGINPILTKHDYPELAVKIGIDEGEDVVVQYGYDKSSLID
                     ILGYSMNVAAKITSLTSANKISVGNAVYKLLHPTIQSKFKLLTINKGWKYVDRETGEL
                     YKVFTMK"
     gene            complement(1647..2288)
                     /locus_tag="VF222_03950"
     CDS             complement(1647..2288)
                     /locus_tag="VF222_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4443 domain-containing protein"
                     /protein_id="HEX7141610.1"
                     /translation="MNTYFRLLEKVSHQSATSRRLSFGIGHIFKALQLAKLNGHISRD
                     LLRKELSLGEGSIKTLVKHLKITNMIMTSNAGTILTKRGEKLISQILLCIPKETVIPK
                     SSITVGKFNYVVLVKNIANSIRSGIEQRDVAIKSGAMGATTLIFKDGKFLISGTNFNA
                     LVEELNVQKTLLENLHPDDNDVIIIGSDNHNKQYAEIAAKNAALFTVINHLKH"
     gene            2407..2622
                     /locus_tag="VF222_03955"
     CDS             2407..2622
                     /locus_tag="VF222_03955"
                     /inference="COORDINATES: protein motif:HMM:NF021038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEX7141611.1"
                     /translation="MSFLKQIQISESQVPKAAIGLLIFILAFGLFIVGYDQGHLFSLV
                     QGEQAYNDLWVHEFSHDMRHAAGFPCH"
     gene            2644..3375
                     /locus_tag="VF222_03960"
     CDS             2644..3375
                     /locus_tag="VF222_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX7141612.1"
                     /translation="MDSLSFLVVTLISGAIAGTIFGLINLVIVEPYLDNAIEIELQNT
                     INAGEKINPKEHNNYRFWQKGGEVAAGTILGMSFGSLLGILFVFGRKTLPGSNNIKKA
                     IILSGIIWLVIFMIPAIKYPPNPPTVGDPDTINYRQYLFISFILISGVSALGLSIIYK
                     KIKTNPLLKLIIVSIIYAVVMIGAFIVVPPSPDNTTAPIDLVNGFRISSMFTMGIFWI
                     ILGFTFGLIWDKLRPHDATQYKKTV"
     gene            complement(3755..4633)
                     /locus_tag="VF222_03965"
     CDS             complement(3755..4633)
                     /locus_tag="VF222_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX7141613.1"
                     /translation="MFTSINKLPNPLPESDGPNPKAAQALQEGLGGQFGEMRTMMQYL
                     FQNINFRGDAKPYQDLLRSIATEEMGHVELISNTINVLLEGSTTQADPNELPLAVALE
                     SPNIHHFLVAGQSCRPVDAAGNPWSATYVYDSGNLVLNMLYNLMLEATGRLQKCRLYE
                     MSDDKAYRATVSYLIVRDLAHEKVFAKALETLGVEWNKTLPVPKVDTSKMPEVREMEE
                     LNLHNQQWTMTNEKSGLSLIFNGESPFKDGQLETLDGLPEGAAIPNLPEAPQEFSPGL
                     DKELKSKFQNLKKNKI"
     gene            complement(4768..>5535)
                     /locus_tag="VF222_03970"
     CDS             complement(4768..>5535)
                     /locus_tag="VF222_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7141614.1"
                     /translation="INITSKIKLGPVITYLFPEYRNITLLAKQAITLQNLSKGRLEFR
                     TGAGATLQYSIQWWYPFGIEYLKAIERVQLLDEGLQVLSLLWNSKISSVKFEGKYFKL
                     NDASINIPYIVIEKIPITVAAKKKKTMAIAANYADIWESSYISPIEFLKLNNEFTRII
                     GKDTNREIEKSIELDVIIAESDTDLKYKEKIFAMERGPNILHQIKKHSLIGKPTDIAE
                     RIKEYLEVGVTQFLLAFQDPFDKSSLSLFNDVMKAVK"
BASE COUNT         1800 a          820 c          807 g         2108 t
ORIGIN      
        1 agcagtatta ctattcagtg ctgtgttttc tttcctcttt gtaggacata caaatggaat
       61 taatttcgct gttgcacaga ctgcaaatac ttccgaccca ggtcctgaga caattcaaga
      121 ttttgagaaa gaaataggag acgataatag tgctattata agaaatgata caaacatcag
      181 taatccaaat aatactcttc ttgatgctac agaagtgaat atcgaagagg attgtatggt
      241 attaaaagat ggaagtaact actgtccata atataaatat ctgctgtcga taaaatataa
      301 tcctacattt ttttattatc atatagtaat attgcagttg tatttatgat acttctggtt
      361 aaatacttca ttcagtagtt aaattccact attagatgca aattataaat ttgttaacaa
      421 gaataataat aataatattt ttatgttcca ctctaaaaat aatcacttca ttgtaaatac
      481 tttataaagt tcacctgttt cacggtcaac gtatttccag cctttattaa tagttaataa
      541 tttaaatttt gattgtatag taggatgtaa taatttatag actgcatttc ctactgatat
      601 tttgtttgct gaagttaatg aagttatctt cgctgcaaca ttcatgctat atcctaagat
      661 atcaattagc gagcttttat catagccata ttgaacgaca acgtcttctc cttcatctat
      721 tcctatttta actgctagtt ctggataatc atgcttggtt aatataggat taattccatt
      781 tttaataaca tttatcattg attttgcaca ttgaacggac ttgtccgata taagatattt
      841 attaaaacca cacggaaaaa aagatattat tgcatcaccg acatatttta aaacataccc
      901 attataactt tctacaactg atgatatctc gtgagaaaat gctctcatta ttgttaccaa
      961 ctgttctacg ggtaatgtca tgctcatttt agttgaacct actaaatcta tatacataac
     1021 aacaagttct acttttgaat taacatgatt aagaagaaat tcttgtccgt gtttaactga
     1081 actattatat tgatatcgtt cttctaatgc tttccagagg cgatcttgtc tttgggaaaa
     1141 taaggtttgt aaatcaacga gttggtcttt tctattttca ttttctttag ttgaactacc
     1201 gattattctg tttaatattt ttgcatttac attgtcaaca tctctttttt gttcagactt
     1261 tttttgaata attacttttc gttctgattt tggctttata ttttttgttt tcatctttga
     1321 agtcaatgtt aattagtaaa aagtagtaat actttaattt aaaattttca atactatagg
     1381 atggaattgg tataagaatt aaaaagattc aagtgaaaaa atggattgag tattatatag
     1441 ttgttagtac tatatcaaaa ttttaatttt taattaataa catatgtgat aaaaattaat
     1501 attagtgaca aaagtcaaat taagggttaa aacatattgg tgtaagatat tatggatgtc
     1561 ataactagta gaaggagaat gttttaaaat aaaaaataaa attaatgtta caaatattca
     1621 tatacaaata agtaaacata tatgaattaa tgtttaagat gattaataac agtaaaaagt
     1681 gcagcatttt ttgccgctat ctctgcatat tgcttattat gattatcact tccaattatg
     1741 atcacatcat tgtcatcagg atgtagattt tctaataatg ttttttgtac attcaactcc
     1801 tcaactaatg cattaaaatt agttccagat attagaaatt ttccatcttt gaatattaat
     1861 gttgtagcac ccatagcacc agatttaata gctacgtctc tttgttctat tccagaacgt
     1921 attgaattag caatattttt tactaaaact acataattaa atttaccgac tgttatagag
     1981 ctttttggaa taactgtttc ctttggaatg cataatagta tttgtgaaat taatttttct
     2041 cctctctttg ttaaaattgt accagcatta cttgtcatta tcatatttgt aatctttaaa
     2101 tgtttaacta gggttttaat ggacccttct cctagtgaaa gttcttttct aagtaaatct
     2161 ctgctaatat gtccattaag ttttgcaagt tgtaaagctt tgaaaatatg tcctatacca
     2221 aatgagagtc gtctagatgt agcactctga tgagaaactt tttctaatag tctaaagtaa
     2281 gtattcatca gttaatttac attaataata ttgaatggtg tatctaaatg tattcaacgt
     2341 ttaactaatc atgttggata ttttagacaa ctgttatata ttagtgactt gataagattc
     2401 aataatatgt catttttaaa acagatacaa atttcagagt ctcaagttcc taaagctgct
     2461 ataggattat tgatattcat attagcattt ggtttattta ttgtaggata tgatcaaggt
     2521 catcttttta gtcttgttca aggagaacaa gcatataatg atctttgggt tcatgaattt
     2581 tctcatgaca tgaggcatgc agcaggattt ccatgtcatt aggttcctac ttttgtgatt
     2641 actatggata gcttatcttt tctcgttgtt actctaattt ccggtgctat tgccggaaca
     2701 atttttggac ttatcaatct agtaatagta gaaccttatt tagataatgc aatagaaata
     2761 gaattacaaa ataccataaa tgctggcgag aaaataaatc ctaaggaaca taataattat
     2821 cgcttctggc aaaagggtgg agaagtagca gctggtacta tattgggaat gtcatttgga
     2881 tctttattag gaatattatt tgtttttggt agaaaaactc tacctggttc taataatatt
     2941 aaaaaggcaa taatattatc aggaattata tggttagtta tttttatgat tccagcaatc
     3001 aaatatcctc caaatccgcc cacagtagga gatcctgata cgataaacta tagacaatat
     3061 ttgtttatct cgtttatttt gatatcaggt gtaagtgcct taggattgtc aataatttac
     3121 aaaaaaataa aaacaaatcc gttattaaaa ctaattattg tttctattat atatgctgta
     3181 gtaatgatag gagcctttat agtagttcct ccaagtccag ataatacaac tgctccaata
     3241 gatctggtaa atgggtttag aatatctagc atgtttacaa tggggatctt ttggattatt
     3301 ttgggattca cattcggttt gatatgggat aaattaaggc cacatgatgc gactcaatac
     3361 aagaagactg tttaatattt ttatgtattt ccctgataac tgtagataaa tatttacaac
     3421 aaacaaaata gtgatgtaat accttttcaa gcgaactttt taatattaat taaaacgtta
     3481 ttgaatgtag aaacatatta ttatcatcac aaacaatttg caactatttt tttagatacc
     3541 tgacccgtaa tttccaagta actttaactt tctctataaa tattgaagat tgataatatt
     3601 cagtgaatta tcaaaaatat aaacttaatt taaataatat aaataacttt ttttattttg
     3661 ggtggtatag ttgaagtcac cttattttat aaatatactt agaatgataa attaatgcaa
     3721 aaataaaata atttattaaa aaaaataatt atttttatat tttatttttc ttcagatttt
     3781 gaaattttga tttgagttct ttatcaagtc caggagaaaa ttcttgtggt gcctccggta
     3841 gatttggtat tgctgcgcct tcaggaagtc catccaatgt ttcaagttga ccatctttaa
     3901 agggagattc tccattaaat attaaagata agcccgactt ttcatttgtc attgtccatt
     3961 gttgattatg caagttcagt tcttccattt ctcttacctc tggcattttt gaagtatcaa
     4021 cctttggaac aggtaatgtt ttgttccatt caactcctaa tgtttctaat gctttggcaa
     4081 atactttttc atgagcgaga tctctaacaa ttaaataaga aacagttgct ctgtatgctt
     4141 tatcgtcact catttcatat aatctacatt tttgaagtct acctgttgcc tctaacatta
     4201 aattataaag catatttaag actaaattcc cactgtcata cacataagtt gcagaccatg
     4261 gatttcctgc agcatctact ggtctacaac tctgtccagc aactagaaaa tgatgtatat
     4321 ttggtgattc aagtgcgaca gcaagtggaa gttcattagg atctgcttgt gtcgttgatc
     4381 cttctaatag gacatttatt gtattagaaa ttaattctac atgacccatt tcttctgttg
     4441 caattgatct caataaatct tgatacggtt ttgcgtctcc tctaaaattt atattctgaa
     4501 acaagtactg catcatagtc ctcatttctc cgaattgtcc cccaagacct tcctgtaaag
     4561 cttgggctgc tttgggatta ggtccatcac tttcaggtag aggatttggt agtttattaa
     4621 ttgatgtaaa catgaacttg tttgttttat aaattatata acgattgcat tatttaatta
     4681 caacaatatt aggattagta ttgttgtcta acagataata ctaatgcaat ttttagtgaa
     4741 gtgaaataaa tccaataatt taatctatca cttaactgct ttcattacat cattaaataa
     4801 acttagtgaa cttttgtcaa acggatcttg aaaagcaagc aagaattgag ttacgccaac
     4861 ttcgaggtat tctttaattc tttctgctat atcagtgggt tttcctatta gactatgttt
     4921 ttttatttga tgcaaaatat ttggacctct ttccattgca aatatttttt ctttatattt
     4981 gagatcggta tctgactccg caattataac atctaactct attgatttct cgatttctcg
     5041 atttgtatct tttccaataa tacgagtaaa ttcattgttt aactttaaaa attctatcgg
     5101 cgagatataa gaagattccc atatatccgc atagttggct gcaatagcca tagtcttttt
     5161 tttcttggca gcaacagtga ttggaatctt ttcaattact atataaggaa tgtttatgct
     5221 ggcatcgttt aatttaaaat attttccttc aaatttaaca gatgatatct ttgagttcca
     5281 cagcaaactc aaaacttgta atccttcatc taatagttgt actctttcaa tggctttaag
     5341 atattcaatt ccaaaaggat accaccattg tatagaatac tgtaatgttg caccggcacc
     5401 tgttctgaat tctagtcttc ctttgcttaa attttgtaat gttattgcct gcttggctaa
     5461 tagagtaata ttacgatatt ctggaaaaag gtatgttata acaggtccaa gctttatttt
     5521 acttgtaata tttat
//
LOCUS       DASVEV010000081        13448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1190729, whole genome shotgun sequence.
ACCESSION   DASVEV010000081 DASVEV010000000
VERSION     DASVEV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13448
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190729"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..567)
                     /locus_tag="VF222_03975"
     CDS             complement(379..567)
                     /locus_tag="VF222_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141615.1"
                     /translation="MGKRFTILLYAAAATTLIAGILHLTKVVDTIGSGDVISNADILF
                     LVGGIAQVFWIIPILTMG"
     gene            759..2021
                     /locus_tag="VF222_03980"
     CDS             759..2021
                     /locus_tag="VF222_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141616.1"
                     /translation="MTTIFFTILVSTAIIFGSLILNVNYTAMAQEQQEQPIINDPNLQ
                     AEIFVLGLSFPTSMAFIDNNNILVLEKEKGTVRLISNGILQEQPVLKVDVASNSERGL
                     LGIAIMNNDMVFLYYTESSSSSEDNESLRNKVYKYQWNEEEKILVNPKLILDLPAIPG
                     PNHDGGKLAIGPDNYLYAVIGDLNHDGKLQNIVDGPQPDDTGVIFRVNPENGSQASDN
                     PFANSENNRYYAYGIRNSFGIDFDPVTGKLWDTENGPQYGDEINLVEPGFNSGWKQVM
                     GPISKSNVIEDELVNFPGSKYADPVFSWLPSIGITDIEFLNSSKLGDKYANNIFVGDI
                     GAQTDGYLYYFQVNEDRTGIKFDSNSQIELTDLTADNEEEMSAIALGTAFGGITDIET
                     GPDGFLYILTVDRESDGKGKIYRLSSSQ"
     gene            complement(2445..3371)
                     /locus_tag="VF222_03985"
     CDS             complement(2445..3371)
                     /locus_tag="VF222_03985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7141617.1"
                     /translation="MEKQNIAFVTGSSTGIGFETSLLLARNGFYTYATMRDTRKGDNI
                     EKIANNENLPLKVLSMDVDNDDSVRNAIHKIIDEKKKIDILVNNAGYGLFGALEEISI
                     DEAKKQFETNLFGAMRTIKETLPTMRKQKNGIIINVTSLAGVVGIPAECIYVSTKFAL
                     EGLSESISYELQPFGIKVIIIEPGVVNTNFVPNIKFSNKTDDKLLQTLMKQEEKSEIN
                     YSNNDIKKKNSSYYSNTIDTFLSHYFSAMNNAPSPKEVANVILEAIKNASNSSEYLFR
                     HTVGEDAKTFAQAKKNMSDSQLHEFISNKLLR"
     gene            3734..4474
                     /locus_tag="VF222_03990"
     CDS             3734..4474
                     /locus_tag="VF222_03990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7141618.1"
                     /translation="MNLAKQDKYTAIVTGSTRGIGKETALLLLKNGINVIISSRSQQS
                     VDDIIQEIHDKLPIKKGKVLGLKCDVSKPYDVKSLVGVSVKRFGKIDILVNNAGIVYF
                     KSIMSTTEEEWNKTIDTNLKGIFLFTKEVLPYMIENKSGGIIINVSSGAGKFGFPNLS
                     AYCASKFGIIGLTESVAKEVADKYVKVMSICPGGVDTKMIEDMVDNGYNLSNRNLMKP
                     EEVAKKIYDMIFNQNDYYNGQSVEFYNK"
     gene            complement(4726..5376)
                     /locus_tag="VF222_03995"
     CDS             complement(4726..5376)
                     /locus_tag="VF222_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141619.1"
                     /translation="MSARKEEIKINENVTVNEPKFNEKNTTKFSTDNNNFNERTNNAL
                     TQQQDVINMTFDNTLNNIKRNTDEATREIPQYTQRIAEYQEQTIQTIKDIASDFIEAE
                     KQVIGSFQSQVDRNSGNTNGLWDMYNPQRIAENYSVMVNKFTSYLLNTSNLINNALAS
                     NMRLYNTALEQTRDNLKACAKTNTNFIQSVSGNSQNRDGNTSNNNNNNINERPTRF"
     gene            complement(5830..6024)
                     /locus_tag="VF222_04000"
     CDS             complement(5830..6024)
                     /locus_tag="VF222_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141620.1"
                     /translation="MFQQRLIIKKESISYCLYLVPDAAIVDDILAIAVLSVVLTMVQT
                     DNTTPAMTEITFNFEDSEII"
     gene            6182..6799
                     /locus_tag="VF222_04005"
     CDS             6182..6799
                     /locus_tag="VF222_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEX7141621.1"
                     /translation="MKIFLLNKRELVYTIGISAILTVSLLTLMIAPQNSFAQPTTSTN
                     MSKPIDGYNLPQGHLTAIRHVFDDPSLRVHHYCKIDEKIFLVCQLYDSHGKNATLIGV
                     EYIITAEQYTTLPDREKPNWHYHKIEFASNRADPKFPELNPEQAKAEMQKAMDTYGKV
                     IITWNPNDLLPAFPPQIQQVEHPFMVNSTVKPETMKGSFNQTLIY"
     gene            8240..8662
                     /locus_tag="VF222_04010"
     CDS             8240..8662
                     /locus_tag="VF222_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141622.1"
                     /translation="MNKSLLIAGIFGFLVSAAIAEIYALYSPSDVLTSTITVLVGFII
                     YKISFIVLFHIDNKRKYTNRFTSRINFNRLKQIIKKMIFAWSIFDIVNNLSRWIILYE
                     LLIIEFRPFEAAIISSIAASSISYLSINIILKRIKIFS"
     gene            8907..9083
                     /locus_tag="VF222_04015"
     CDS             8907..9083
                     /locus_tag="VF222_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141623.1"
                     /translation="MSDEDRTKEERGQALGKEERNQKKVETDSSNNLNEDRTKEERGQ
                     ALGKEERNQKKVET"
     gene            complement(9585..10886)
                     /locus_tag="VF222_04020"
     CDS             complement(9585..10886)
                     /locus_tag="VF222_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141624.1"
                     /translation="MPKSVFTFGLLLGSLVMLGVMPFFNLNDAANATRAAPIPEGPII
                     NDSNLKAEVVFEGLTSPTSIAFLGPNDILVLEKDKGTVQRIVNGKILTEPLLEVNVAN
                     DGNRGMLGIAVSKGEEIGPTNIFLYFTESPLGEFPIGNRVYKYELVNNKLVNPTLLLN
                     LPAIPGPSHNGGAIMIGPDNNIYTIIGDVGGHRNQAQNQESGGEPDGTSGMLRINQDG
                     MPVAISPLGDREPLNLYYSYGLRNSFGIDFDSVTGKLWNTENGEDFGDEINLVEPGFN
                     SGWNKVQGIWTLDKEEHMTKVAPLHPENGLVNFGGKGKYSPPEFTWHESLGPSAIKFL
                     NSNRLGIQYENDIFVGDIDKGNLYHFDLNEDRTQLVLEGSLADKVANADDENEGIIFG
                     QGFGAITDIEVGPDGYLYIVGFQGTIYRIVPIQELSTDNLT"
     gene            complement(11504..12004)
                     /locus_tag="VF222_04025"
     CDS             complement(11504..12004)
                     /locus_tag="VF222_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141625.1"
                     /translation="MLDTNTLDYIYTHQILLVSKLKSFSKKHIHLYITHVQQDEINKM
                     KDISKKSCINKIISIIGIKRVLTASTVIGIDENKRGFTGSSIDMYELVDDIDLHVLEK
                     LQKHTPSNPMGNTADLSILYTAVKKKMDYLITDDTSDFEPMLKEMSKFIPNYLQLRKN
                     SDLNYF"
     gene            complement(12331..12639)
                     /locus_tag="VF222_04030"
     CDS             complement(12331..12639)
                     /locus_tag="VF222_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX7141626.1"
                     /translation="MKCLSLKQPFADLLAFGEKTIELRKWNTKFRGEFLIHASKNIDM
                     QSCERLDIDIDKLTIGAIIGSAFVYDVKEYSNQEDFNKDKQKHFSIISKYLEATNMVS
                     "
     gene            12805..12963
                     /locus_tag="VF222_04035"
     CDS             12805..12963
                     /locus_tag="VF222_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141627.1"
                     /translation="MPNLDPWINQNNSNKTKEYVATKTITNKFENHLLSIKDEHQKRI
                     KTQFTSYL"
BASE COUNT         4780 a         1846 c         1937 g         4885 t
ORIGIN      
        1 ttaagaatat gtaacttatt attccaacca atattgtatt ttattattca tgtatagcat
       61 gtatagaact ttttcttcac actataaaat tattttacat ttcaaaactt aaagatgttt
      121 cgtagagaat tttacataaa tattaattag aatcaaagga aattttaatt ggttttaatc
      181 ttattattcc caacctttat acttttaatt aatataatca ggctcaggat aataaaagca
      241 atttgaaata cttgtattat tattgcttct tcacttattg aaccgccttt tcctgttatt
      301 ggattatcag gtatcctggt aattacccat attagtacaa atacgactgt tcctgctatc
      361 cctatagaat accaagtttt accccattgt aagtatagga ataatccaaa atacttgggc
      421 tattcctcca actaaaaaca aaatatcagc gttactaata acatcaccac taccgatagt
      481 atctacaacc ttagttagat gcaatattcc tgcaattaaa gtagttgcag cagctgcata
      541 tagtaatata gtaaaccttt tccccattct ttaaatactg atttgtaaca aactataaaa
      601 aagaattagc caatttctat atctttaaaa cttaatttat gaaaaggatc attcttcttg
      661 aaagttactg tagaattaat accatgttta ttaaatacta atacgtaaat tttataatgt
      721 atgaaaaagg caacttttca tttagctttt cattttgtgt gacaacaata ttttttacta
      781 ttcttgtttc gacagctata atttttggct cactaatatt aaacgtaaat tatactgcga
      841 tggcacaaga acaacaagaa caaccaatta ttaatgatcc taatttacaa gcagaaattt
      901 ttgtattagg actatcattt cctaccagca tggcatttat agataataac aatattttgg
      961 ttttagaaaa ggaaaaggga actgtacgac ttatttcaaa tggtatattg caagagcagc
     1021 cagtattgaa agtagatgta gctagtaaca gtgaacgtgg acttttggga attgcgataa
     1081 tgaataatga tatggtattt ctatattata cagaatcctc ctcttcctcc gaagataacg
     1141 aatcattaag gaataaagtt tacaaatatc aatggaatga agaagaaaaa atacttgtta
     1201 atcccaagtt aattttagat ttacctgcaa ttcctggtcc aaaccatgat ggtggaaaat
     1261 tggctattgg acctgataac tatctttatg cagtaatagg agaccttaat catgatggta
     1321 aactacagaa tattgtagat ggaccacaac ctgatgatac tggagtaata ttcagagtaa
     1381 atccggagaa tggctctcaa gcctctgata atccttttgc aaatagtgag aataatagat
     1441 attatgctta tggaatacgt aatagttttg ggattgattt tgatccagta accggaaaac
     1501 tatgggatac cgaaaatgga ccgcaatatg gtgatgaaat taatttagtc gaaccaggtt
     1561 ttaacagtgg atggaagcaa gttatgggtc ctatatccaa aagcaacgtt attgaagatg
     1621 aattggttaa ctttccagga tcaaaatatg ctgatcctgt atttagttgg ctcccatcaa
     1681 ttggaattac tgatattgaa tttctaaatt cgtcaaagct tggagataaa tatgcgaaca
     1741 atatttttgt tggagatatt ggggcacaaa ctgatggata tctctattat tttcaagtta
     1801 atgaagacag aacgggaata aaatttgata gtaatagtca aatagaacta actgacctta
     1861 ccgccgacaa cgaagaggaa atgtctgcaa tagcccttgg aacggccttt ggtggcataa
     1921 cagatattga aactggacca gacggatttt tgtatatact cacagtagat agagaatcag
     1981 atggtaaagg gaagatctat agactttcat catctcaatg atattttatt tttgtagtaa
     2041 acctgcgaat cattattttg ttttcataga atacagtatt ttacttatca tccaaaaaca
     2101 aaaaattggt catactgtta tctctgcaac tacacctatt tattgtcaat aatgatcaga
     2161 tttagattca tttttttaat ctaatattta ttaataaata atatgttata acttgaatat
     2221 ttgttaagca atctggtcgg taataaagtt ctgcatgcag tagggttcta ctaccttata
     2281 tttagctttt attattagaa cttaatcaaa atttttttta attataagaa ggtaaaggaa
     2341 tttgaaatat accaaggagg aagtaatacc gagcggtttt aaaataagag tctaatgtta
     2401 tagtaaataa aataatattt aagaattctt ttatgatagt atacttatct taacaatttg
     2461 tttgaaataa attcatgcaa ttgtgaatca gacatattct tttttgcttg tgcaaaagtt
     2521 ttagcatctt ctcctactgt atgtctgaac aaatattctg aagaatttga agcattttta
     2581 attgcttcaa gtattacatt agcaacttct tttggtgatg gagcattatt catagctgaa
     2641 aaataatgag atagaaatgt atctatagta ttggaataat atgaagaatt ctttttctta
     2701 atatcattgt tagagtagtt aatttctgat ttttcttctt gtttcattaa agtttgtaat
     2761 aatttatcat cagttttatt tgagaattta atatttggca caaaattagt attgacaaca
     2821 cctggttcaa taatgattac tttaattcca aatggctgaa gctcatagga tatagattca
     2881 cttaatcctt ctaatgcaaa ttttgtactt acataaatgc attctgcagg tattcctact
     2941 acccctgcaa gagaagttac atttatgatt atcccatttt tttgttttct cattgtgggc
     3001 aaagtttctt taatagttct cattgctcca aagagatttg tttcgaactg tttctttgct
     3061 tcgtctatag atatctcttc caaagcacca aataaaccat aaccagcatt atttaccaaa
     3121 atgtcaattt tctttttttc atcaattatt ttgtgtattg catttcttac tgaatcatca
     3181 ttatccacat ccattgataa tacttttaaa ggaagattct cattatttgc aattttttca
     3241 atattatcac ccttacgtgt atctcgcatt gtagcatatg tataaaatcc atttcttgca
     3301 agaagtaatg aagtttcaaa tcctattccg gttgaactac cggtaacaaa tgcaatattc
     3361 tgcttttcca ttctaatcct aggcaattac aaaatataca aaaatataat tctatagttg
     3421 acgataacaa tagatttatg gtaattattg ttgttttttg aaatagttct aattcattat
     3481 aagtaggaat taaacggact atgtatctta ctttaatctt ttagtttctt ctaaatattt
     3541 cataatatta taatcgggtc cactttagaa tagtagtagg gttcatctac atatttagca
     3601 agctggtcgg aagcggattc gtccatgtag taggggttct actacattgt agtatagatt
     3661 tcagatgtat ctacataatt taataactaa tttaaaatta caatattaaa ttaaaataat
     3721 actgaaacta attatgaatt tagcgaaaca agacaaatat actgctattg ttactggtag
     3781 tacaaggggt ataggtaaag aaactgcatt acttttacta aaaaatggaa taaatgtaat
     3841 tatatcctcc agaagtcaac aaagtgtaga tgatatcata caagaaattc atgataaact
     3901 tcccattaaa aaaggaaagg ttttaggttt aaagtgtgat gttagcaaac catatgatgt
     3961 aaaatctctt gttggtgtat cagtaaaaag atttggaaag attgatattc ttgttaataa
     4021 tgcgggtata gtttatttta aaagtataat gagtacaact gaagaagaat ggaacaaaac
     4081 tattgatact aatttgaaag gtatttttct atttaccaaa gaagttcttc cttatatgat
     4141 tgaaaataaa tctggtggta ttattattaa tgttagttca ggagctggaa aatttggttt
     4201 tccaaactta tctgcatatt gtgcaagtaa atttggtata attggtctga ctgagagtgt
     4261 agctaaagaa gttgctgata aatatgtaaa agttatgtca atatgtcctg ggggagtaga
     4321 tacaaagatg atagaggata tggttgataa tggatataat ctatccaata gaaatttgat
     4381 gaaacctgaa gaagttgcaa aaaagattta tgacatgata tttaatcaga atgattacta
     4441 taatgggcaa tcagtagaat tttataataa atgaataata aatatttgtc aaatgtcaaa
     4501 tgattctata gtattgttat agtaataccg atagcggtgc taaaataaga gtctattgta
     4561 aagattacta ccttgtattt agcttttaat aattttattt gtttgaaata aatggaatag
     4621 tataacatga atctaaaatt gacctttcat gtcttaaaaa ataaagtaaa gtaaagtaaa
     4681 gattgattag gtacaataat tcaaatttca gattgtaggt taaaattaaa acctagttgg
     4741 tctttcgttg atattgttgt tgttgttgtt gctagtattt ccgtctctat tttggctatt
     4801 acctgatact gattgtatga aattggtgtt tgtttttgca caagccttta gattatcacg
     4861 tgtttgttca agtgctgtat tgtatagtct catatttgat gctagagcat tatttattaa
     4921 attgctagta tttagcaggt aacttgtaaa cttatttacc attacggagt aattttcagc
     4981 gattctttgt ggattataca tatcccataa tccgttggta tttccactat ttctatctac
     5041 ttgtgattga aaagagccta ttacttgttt ttctgcttca ataaagtcag aagcaatatc
     5101 ttttatagtt tgaatagttt gttcttgata ttcagcaata cgttgtgtat attgtggaat
     5161 ctctcgtgta gcttcatctg tatttctttt tatattattt aaggtgttgt caaatgtcat
     5221 atttattaca tcttgttgtt gagttaaggc attatttgtt ctttcattga aattattgtt
     5281 gtctgtgcta aatttagttg tatttttttc attaaatttt ggttcattta cagttacatt
     5341 ttcgttaatt tttatttctt cttttcttgc tgacatgatt atatatacgt aactaaggta
     5401 tataaagtca tgtattaatg actagcgtat agtcattttt gtttatttac aattgattta
     5461 cttatttgta taatttgaag taatatattc agaataaaaa caataacaag cagaatatct
     5521 aggtattata aacaagtcag gaatattacc taaagcggta caaaaataag agtctaatat
     5581 ttagtaataa ttaggataat atttaagatt ttagctttca tttaatatat taaagcaaaa
     5641 tatatttaaa ataaaaaaag agtaagattg tgttatggtg aattgtcaaa acaataggag
     5701 aaagtcctac aatagctgca attccagcag ctcaaaaaaa gattgcaata gtaattcctt
     5761 ctatactgcc ttgagatttt ctatatgaag gatttttgta acaattaaca tcgaacttac
     5821 caataacgct caaataatct cagaatcttc aaaattaaac gtaatttccg tcatagcggg
     5881 tgtagtatta tctgtttgta ccatagtaag aactacagat aaaactgcta ttgctaaaat
     5941 atcgtctact atggcagcat caggaactag atatagacaa tacgagattg attctttttt
     6001 tattattaat ctctgttgga acaataatat agctagagcc atgacagcag gcggtcaata
     6061 cagacccaat gccgtgctga atatcctgag gttacgagta aatgtaattc cctctagata
     6121 cctgagtgag ttttaatttt tttgtattgt ctttttaaac tatgttgaga ttgataaatt
     6181 aatgaaaata ttcctattaa ataaacgaga attggtatat actattggaa tatccgcaat
     6241 actaactgtt tcacttctaa ccttgatgat agcaccacaa aattcttttg cacaaccaac
     6301 aacatcgact aatatgtcaa aaccaattga tggttacaac ttacctcaag gtcatttaac
     6361 tgcaatacga catgtatttg atgacccgag tttaagggta catcattatt gtaaaattga
     6421 tgaaaaaatc tttcttgtat gtcaactcta tgatagtcat ggcaagaatg caacacttat
     6481 tggagtagaa tatattataa ctgcagaaca atacacaaca ttacctgata gagaaaaacc
     6541 aaattggcat tatcataaaa ttgaatttgc atcgaatagg gcagatccta aatttccaga
     6601 actaaatcca gaacaagcta aagctgaaat gcaaaaggca atggacacat atggtaaggt
     6661 aataataaca tggaatccta acgacctgtt acctgcattt ccaccacaaa tacaacaagt
     6721 agagcatcca tttatggtca actcaacagt caaaccagaa acaatgaaag gtagctttaa
     6781 tcaaacatta atttattaat aacaattttt taattttttt aatatttctt catcattagt
     6841 ttctatctat acactttcaa aatattgttt gcttcttttt taatctttac caataagttt
     6901 tgtctttcca atttaatata tatattttat tatacttaat tataaataat agcagtaatt
     6961 aatataaaat attattggat aaataaaatt agtattatgg tattagtgtc ttcatttttt
     7021 aattttgttt tgtattatca gaaggtataa aaatttgaaa tataccaagg agggagtaat
     7081 accgatagca gttctaaaat aagagtctaa tgcatggtaa taagagatag aatttaagat
     7141 ttactttcat tatttgtaag agttattata aagattaata ctgtattttt tagtataacg
     7201 aactgactca gaaatatcat ctccacatat cctttactct acaacacatt atctaattct
     7261 agttatagat taggacatgt atctccactt ttaataaagt tatactgttt tagcaccgag
     7321 atgtcattca aggtatagca tttttcgttg tgtttgttgt gtaacgatgt atgtttttcc
     7381 cattttcatc ctgcactaac catcctatct cttttagaaa ttctattatc gatagaacaa
     7441 actttttatt gtgatttcct tcgtttacga tatagcctat gccaagactt ttattattat
     7501 tacttaaggt atgtatggct agttctagtt ctttaaatct ttcagaaaag taaacgtcag
     7561 tggagaattt tagcgtatct attcatctta cccaatattc gcatgactta caatagttat
     7621 acattataga gaaatgataa ataactcatt gcatttaaac cattgtaaag tgattaatgt
     7681 tgatataagt aatttcaata ctattagtta attattagaa acaaaagttt atctttaata
     7741 cacatacaat tattattaat tcttaaattt ccgtattaat aactaaataa ttacataatt
     7801 aaatttaact atggtagtat tagtcttgct tataggaaag tgttttcaga ttgataatat
     7861 ggtggttacg aatagtcata taagaagaga tacaccacta tttctaaaag aacggattca
     7921 acaaactaag gaagttagtc gggagtggat tcgtccatgt agtaggattc tactacaata
     7981 gtatttagtt ttttttattt tgattatagt tttagtgtct ttttatggtt gagttattta
     8041 tcatagtagt tcattataca ttcacaattg acttttccca aactgaagta tttgtagtta
     8101 tataattttg aaaaaaaagt tttattttta ggttatctaa ttatctattc aaagacataa
     8161 agagttgtct gtgtatatat aattattatt gaataaactc tttaataatt tttacaagaa
     8221 atatagagaa accattttga tgaataagag tttacttatc gcaggcattt ttggattttt
     8281 agtgagtgct gctatagctg aaatatatgc actatattct cctagtgatg tattaacttc
     8341 tacaataaca gtgttggtag gatttataat ttataagata tcatttatag ttcttttcca
     8401 tattgacaac aaacgaaaat atactaatag atttactagc cggattaatt ttaatcgtct
     8461 taaacaaata ataaagaaga tgatatttgc atggtctata tttgatattg ttaataatct
     8521 atcaagatgg ataatactat atgaattatt aataatagaa ttcagacctt ttgaagcagc
     8581 cataatatct tccatagctg cttcttccat atcttattta tcaatcaata taattttaaa
     8641 acgtatcaaa atcttttcat aaccatcaaa aagaagtata ttaaaatgaa attattagtg
     8701 atataaatag gcacagaagg gaatgatgtc tatagcgatg cgaaaataag agtctaatat
     8761 catagtaata tggatatgtt tatttagttt tgtattccaa ttgttgatgt taaacaaata
     8821 aggttattaa acaaataatt aaaattttat aacgcacata ggaaaccaag ataaatactt
     8881 aattatcata aaaactatat aaaaatatgt cagacgaaga tagaactaaa gaagagagag
     8941 gtcaagctct aggaaaagag gaaaggaatc aaaagaaagt agaaacagac tcatcaaata
     9001 atttgaatga ggatagaact aaagaagaga gaggtcaagc tctaggaaaa gaggaaagga
     9061 atcaaaagaa agtagaaaca tagtaatact cacgctcatt atttgttata gcattttagt
     9121 tagttatcaa tgatttactt gtttcgtcaa gcaaaagtga gtcaatctta tttttcaact
     9181 ctcttagtaa aatgggtttg tatataacaa ttttttcaac atcgagaatg cctttttgta
     9241 attcttgaat agagttttta tcagcaggaa taaagcaaac tattactgta tcatcaatct
     9301 cttagaatta aaatttgact tcttttctaa tacaactttt tttaatagat tttataaata
     9361 ttgtaaataa taatttcaaa ataaattaaa ataataacag aaatttacaa aaatagtaga
     9421 tcagaatcta aagatagaaa ttttgaattt aatgatataa atataaaata catgatggag
     9481 aagagtatca aattacatat gttttacatt atatcaaaag aaaaaagaga gaaaggtaat
     9541 tgattgtcaa ggtgaaaaga aagatgaaga gagtacattt actgctaagt caagttatca
     9601 gttgaaagtt cttggatagg aactattcta tatattgttc cttggaatcc aacaatatag
     9661 aggtaaccat caggtccaac ttcaatatca gtaattgcgc caaaaccttg tccaaatatt
     9721 atcccttcat tttcgtcatc agcatttgcc actttatctg caagtgaccc ttctagaact
     9781 agttgtgttc tatcttcgtt taaatcaaaa tggtaaagat ttcctttatc tatatctcct
     9841 acaaatatat cattttcata ttgtataccc aatctattgg aattaaggaa cttgattgca
     9901 cttggaccaa gagattcatg ccatgtaaat tctggaggac tgtattttcc ttttccgcca
     9961 aaatttacca agccattttc aggatgtagt ggagctactt tagtcatatg ttcttcttta
    10021 tctagtgtcc atataccctg caccttattc catccgctat taaaccctgg ttcaacaaga
    10081 ttaatttcat caccaaagtc ttcaccattt tctgtattcc atagtttacc tgttacagaa
    10141 tcaaaatcaa ttccaaagct gtttcttaac ccatatgaat agtataagtt taaaggttct
    10201 ctatctccta gaggactgat tgctacaggc attccatctt gattaatcct taacataccg
    10261 ctagttccat caggttctcc gccagattcc tgattctgag cttgatttct atggccacca
    10321 acgtctccaa taatggtata gatattatta tcaggtccaa tcataatagc accaccatta
    10381 tgacttggtc caggtatagc tggtagattt aaaagtagtg taggatttac taatttgttg
    10441 tttactagtt catacttgta aacacgattg cctatcggaa attcgcctag gggagattct
    10501 gtaaaatata agaaaatatt tgtaggtccg atttcttctc cttttgatac agcaattcct
    10561 aacatacctc tattaccgtc attggcaaca ttgacttcaa gtaatggttc tgtcaatatc
    10621 ttaccattta caatcctctg tactgtacct ttatctttct ctaaaactaa aatatcattt
    10681 ggacctaaaa acgcaatact ggtaggggat gtaagacctt caaagactac ttctgccttt
    10741 aaattagaat cattaataat tggtccttca ggaattggtg cagcacgtgt ggcgttagca
    10801 gcatcattta ggttgaaaaa tggcattact cctaacatta ccaatgaacc taacaaaagt
    10861 ccaaaggtaa atactgactt gggcatatct ttgtttaaac agttaaaata taactgaagt
    10921 tctttggtat acttgctgac attgtattac taaattagct tagtgaatgt ttattgttat
    10981 ttcatttagt aattattctt aataaattat ttctaaaaat attataacat taaatactaa
    11041 ttaattttat atagcataga tactctaact ttccaaaaag aagttgttaa ataatgaatt
    11101 tttgtgttag ttagatttac atttaacaat atggttcaca taacttttat ttacctttta
    11161 ttattataat cttatcttaa tataagtatt agaaatgtga aatgtgattt actattttaa
    11221 tattaatacc acaataaaag ttaacctaac tataataaaa tattcttcat attaattaca
    11281 aattaaaatt agaacggttc tatcactaca ttgtattagc ttttattatt agaacttatt
    11341 ttttaaaatg ttttttggaa tattaagata ggtaaattcg ttagaaattt gcgaaggagg
    11401 agtaaaaccg aaaaacggtg caaaaattag agtctaatgt atggtaataa agtgaataat
    11461 gtttaaagat tatttataat tgtgaattat actacttaca aatttaaaaa taatttaaat
    11521 cactattttt tctaagctgt aaataattcg gtataaattt gctcatttct tttaacatag
    11581 gttcaaaatc agatgtatca tctgttatta gataatccat ttttttcttt actgcagtat
    11641 ataaaattga taaatcagca gtattcccca taggattaga aggagtgtgt ttttggagtt
    11701 tttccaagac atgtaaatca atatcatcta ctaattcgta catgtcaata gatgaaccag
    11761 taaatccacg tttattttca tcaataccta taactgtact tgcagtaaga actcttttaa
    11821 taccgataat tgatattatt ttatttatac aacttttttt agaaatatcc ttcattttat
    11881 taatttcgtc ttgctgcaca tgagtaatat acaaatgaat gtgtttttta gaaaaactct
    11941 taagttttga tactaatagt atctgatggg tatagatgta gtctaaagta ttagtatcaa
    12001 gcattatatc atacaacaaa ttatcacaac atattttaga ataagaattt tttgaatgag
    12061 ttatttctct aatatgaaat tgttattaac gaatgatgtc atctaaaaat tcaatgacat
    12121 tcatcgtttt tgggagttta ttgaagaaat ttgtattaga ctatcagaac aatttcgatt
    12181 ggtacaagaa atagtgagat tgatatctta atgacaacaa cggttatttt acaatttaat
    12241 atttggtatt tagtagagaa tcatttactt caaagaattt caatttaccg gaataaatga
    12301 ttggtttttt aaacatttta ccatttctaa ttaagaaacc atatttgtag cttcaagata
    12361 tttactaata atagaaaaat gtttttgttt atctttgtta aaatcttctt gattactata
    12421 ctctttaaca tcgtatacaa aagcagaacc aattattgca cctattgtaa gtttgtcaat
    12481 atcaatatct aatctttcac aagattgcat atcaatattt ttagatgcat gaataagaaa
    12541 ttctcctcta aatttagtat tccattttct taattctatt gtcttttctc caaacgctaa
    12601 taagtctgcg aagggttgct ttaaagataa acatttcatt aatataatat ctttatctaa
    12661 ctatcaattt ctaatataat tattattatt attattatta taattgtcaa atatgtagca
    12721 aacttaccat aagtactatc tctccttccc tctcttcatg tatctatcaa cttgataaat
    12781 gacctttttg tgtatgttgt aaaattgccc aatcttgacc catggataaa tcaaaataat
    12841 agtaataaaa ctaaagaata tgtcgctaca aagacaataa caaacaaatt tgaaaatcat
    12901 ttgctatcta ttaaagatga acatcagaag cgaataaaga cccaatttac atcctaccta
    12961 taatgaacgt catgaaaaga ttatagaaat gatagattgg actttggaga aatataaaga
    13021 agcagcagcc accaaaaaaa aagaaccatc agaatgaaga tgaacaagaa gaagtaataa
    13081 tagataatat cgtcgaggaa ctggataaaa aaagggatat agctataaac aaaaagaaaa
    13141 aagatttact aaaagatgaa gtgtctattt atagtttaaa agaaactact atggattata
    13201 ttcttttcat aatcagtgaa tagacagata aattatatta aaataaaagt aaaagaaaat
    13261 aattaagact tattctatta ataatacttt attatcaaga tattttaatt gacatgaagg
    13321 ttaattttac acttgtacca taaatttatt atttatgata atattatgta ctatgccttg
    13381 gaaacacctt gtatactatt tttattttta ttaattattt taggatcacg catttcaatt
    13441 aacacact
//
LOCUS       DASVEV010000082        25019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1190946, whole genome shotgun sequence.
ACCESSION   DASVEV010000082 DASVEV010000000
VERSION     DASVEV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 25019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25019
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190946"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VF222_04040"
     CDS             complement(<1..1002)
                     /locus_tag="VF222_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141628.1"
                     /translation="MKFLFIAVLIGTLAISLMDMQTISIAYAQTSDKSFKGNEAPFIP
                     PPESDRTPKAPPSDAISIKDRPSIGVEDTDEEEEEEIDKDRLPPLTAREIQELSFDTI
                     NDPEFREVKIIPNETSFSDPIRSLVQEIIKNETIISNDNDIISFNSSKIKNKFDDTQL
                     GQIFIAQNMSRDSETSLEGNDTEIISNTTADHDDGDNIFSETNLPNRDILNFINSTKV
                     GSNTTEAEGQPAITDDLSRQGNVTEAEGQPAITDDLSRQGNVTNNNQQNNEEELLQGG
                     KTITIEEIPLKSADSQEEEATEGNVTIKGQPAITDTTEEPGMTTSSELQDEGQQQQQP
                     "
     gene            complement(1125..1796)
                     /locus_tag="VF222_04045"
     CDS             complement(1125..1796)
                     /locus_tag="VF222_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141629.1"
                     /translation="MRNNLIVFLVMGLSLLILGIINTDNLIPVFAAIIVGTEEEDEIK
                     GTNKKDSLFGLEEKDKLWGRDASDYIDGGEGRDKIEGNSGKDKLYGNSGSDRIEGNKG
                     SDYIDGYTGNDLIQGNSGGDNILGFNGDDIIQGNKDDDILYGEKQNDRIEGNSGNDWI
                     NGGQGRDLLIGGSGDDILWGKSGKDVMIGGTGADEFHCGQGFDIILDFDKFEGDTKSK
                     NCEVF"
     gene            complement(2063..2851)
                     /gene="lsrF"
                     /locus_tag="VF222_04050"
     CDS             complement(2063..2851)
                     /gene="lsrF"
                     /locus_tag="VF222_04050"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEX7141630.1"
                     /translation="MKNRFSRIIKPKNGRCVMLAVDHGYFLGPTEKLEVPQKTIQPLL
                     PYVDSIMLTRGVLRTSVTPTSNVPIVLRVSGGTSIIGEDLSKETIVTSMEDAIRLNVS
                     CLALSIFVGSKYEHQTLSNLAALVDQGERYGIPILAVTAVGKEMVRDARYLGLACRIA
                     GELGAHLVKTYYCEKFEKVIEGCPVPVIIAGGKKLEKEIDVLELTFNAIREGASGVDM
                     GRNIWQSDSPIAMIKAVKSIVHENFSIKEASDLLMLKKKIEIKR"
     gene            complement(2926..3966)
                     /locus_tag="VF222_04055"
     CDS             complement(2926..3966)
                     /locus_tag="VF222_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7141631.1"
                     /translation="MKSVKIVSQGVVQVYELDDNVLEFKSDGDLLVEMKACGICGSDL
                     EKVYGQYGMTSARLGHEPAGEVLSIGNNVKGITVGDRVFVHHHVSCNSCHYCRHGNNT
                     MCEFYQKSNLDPCGLADKFIVPAWNVERGGVLKLPDNLTYEQASLIEPLACCIRALNK
                     IPFQKGDDLVIFGAGPAGIMLLNLARMYGAGKIFVVDINDFRLEFSQKFVSDAIFINS
                     LNENYNKIIKDKTKGRGGDISIVATSSLTALSNAFEITRKGGYIILFGVPSKDSEFPL
                     EASKLYSNEFSLVPSYAASEIETNQALKLLAEGRLNLDFLITHKYSLEESPDAVFCAH
                     KAIDCMKVIVTT"
     gene            complement(4000..4143)
                     /locus_tag="VF222_04060"
     CDS             complement(4000..4143)
                     /locus_tag="VF222_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX7141632.1"
                     /translation="MPVDPVCGIEMEEELAISLEYNDKKYYFCCEGCKGIFKRKPKKY
                     HNS"
     gene            5190..5360
                     /locus_tag="VF222_04065"
     CDS             5190..5360
                     /locus_tag="VF222_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141633.1"
                     /translation="MSYPCLEVVCGNCGSTINRMINLKSIRDSMRPSSGRCNKCGISL
                     NPSDFSLNTVKQ"
     gene            complement(5466..6359)
                     /locus_tag="VF222_04070"
     CDS             complement(5466..6359)
                     /locus_tag="VF222_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX7141634.1"
                     /translation="MNISIWLRIIRIKFLLASIIAITNGLLYTYFTYNQFNIIDALLT
                     YIGVIFLHASVDLFNDYWDYKRGIDTTTKRTTFSGGTGVLPEKKLDPSTVYKAGFICM
                     VIGLSVGGYFVFHSGFIIAIILAVATISIYFYSTTIVNIGLGETLVAVKGSMIVIGTS
                     YVQSGIIDLPSVLLGIIIGALSSIVLYIASFPDFDADKEKGRKTLVILLRKDKASKIF
                     PLLISLIYSLIAIGIIIQYLPLYSIITFSSLLFAIRSSQLLKNYDKIEDLVKAISYTI
                     TFSRTIGICLIFSYIIAIINK"
     gene            6618..7040
                     /locus_tag="VF222_04075"
     CDS             6618..7040
                     /locus_tag="VF222_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX7141635.1"
                     /translation="MSILFTVDKANTILPKVKKRFDEILCCKNNVMDIQEELQNLSDS
                     NCSFEKFIKKKQELNHAVTSLYNMIQELEDLGIMIKSVDEGLLDFPSIRDDEEIWLCW
                     KFGENQVKFWHRKEEGFMGRKPIPQEGFEYVDDFADLR"
     gene            7315..8769
                     /locus_tag="VF222_04080"
     CDS             7315..8769
                     /locus_tag="VF222_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX7141636.1"
                     /translation="MKIIQNERNLLQHHSRFSSFNISISALKKAIKSVDPCVLIKRAI
                     DIKENKLVVVRDVNDVNYFFDFKDYESFHIVGAGKATARMAKSLMETLNIEISSGAIT
                     IPYNEKILPDKLIVTEAGHPVPDKNSIRGTKQMIDLLKKIETKSLVFVLISGGSSSLM
                     CYPLDGLELKSKQVLTKLLLKSGASINEINTVRKHLSKIKGGRLPKYVNEKARIITLI
                     LSDVVGDDLSIIGSGPTVGDNSTFANCIDILKKYDIWNTNNNENPLEISNVKKVLEDG
                     KKGIIEETPPLHHPIYERVSNILIGNNEVACKSAMNELLKFEIKTNYIGSHINSPAKV
                     FGKELAQLLKSPQTKPFNHKEENGSFAYVMGGETVVNLTSTNNSNNNVEGKGGRNQEA
                     IISAINYLHNIKNLDNFTIICAGTDGIDGNSESAGGLVSPSTLESIKKAKINVNFYLN
                     SHDSETLLKKVNSNIITGRTGTNVNDIAIICKIE"
     gene            complement(8943..9767)
                     /locus_tag="VF222_04085"
     CDS             complement(8943..9767)
                     /locus_tag="VF222_04085"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7141637.1"
                     /translation="MGSPYKKMDLGLQNHVIGKRIYHYKKLRSTQQLAISLAETNIKN
                     EHGTVIVADEQDEGLGRNRKKWISPIGGLWMSLIIKPRIEFTKINMMSVISAISVCEA
                     INEISKLKTYVKWPNDVLINDEKIAGILINSSTNNSDKDYVVIGIGINIDIDIAKINL
                     SNASNSIMSWKVTSIQNEIKGKGIDRFTLLKQLLVKIEFYLSLLEYDKYHTRIIDIYK
                     HLINTLGKRIIVYQDDLKKYSAIVKDIDTDGGLILAKEDNSLEKIYSGEISIREKK"
     gene            complement(10004..10144)
                     /locus_tag="VF222_04090"
     CDS             complement(10004..10144)
                     /locus_tag="VF222_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141638.1"
                     /translation="MVKCNIESAYAFEDSFNEKNKSKIKQMWTLSSNLVSEYNGLANF
                     LL"
     gene            11304..11474
                     /locus_tag="VF222_04095"
     CDS             11304..11474
                     /locus_tag="VF222_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141639.1"
                     /translation="MRKVIETIRKSNTAKNSDERVIIDNNNNKHLEFFMNGSWRHSHK
                     CKTVVNNQCIKK"
     gene            11867..12106
                     /locus_tag="VF222_04100"
     CDS             11867..12106
                     /locus_tag="VF222_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX7141640.1"
                     /translation="MSNTSNEQYGDNNDQRNFTNKPVELGKEYNVQITETSYKGDGVA
                     RVQGFVIFVKDGKVGNDVKIKITSIGNRFAKADLV"
     gene            13687..13920
                     /locus_tag="VF222_04105"
     CDS             13687..13920
                     /locus_tag="VF222_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141641.1"
                     /translation="MKQVYLVTVQIEGMVSQLKNIVFEDESACDKFIDKLKSQSPSKN
                     RYLCYKWKIPLITNEDYNNLNENEVLENSKELI"
     gene            14270..15760
                     /locus_tag="VF222_04110"
     CDS             14270..15760
                     /locus_tag="VF222_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7141642.1"
                     /translation="MNTPLSISQLKRLNHFHIQKLNTKKNQFQIYSDIFNFYDSTFIF
                     ESLSGPKELAETSIIGFSPSFTIKCNSKNLIIFNKNKKKVKTLKVKEPLSQLRSILPK
                     ITLHDFNYRYIGGAVGYISYEAISFWERLPAPKKKILNFPLFEFGIYTDGILYDHKEN
                     QAYYFHTGLESQIDEINKILNNDCHPFNANTELNWKNRHHNKNLKLKNIKCSVPQRNL
                     SKAEFIKMVKKAQSYIYDGDIYQVVLSKNFKFTIEGNLLQVYSSLRKINPSPYMYLFK
                     IDKRCIIGSSPEMLLRVTGKVIETFPIAGTRPIGENEEETTKLGENLLKDEKELAEHT
                     MLVDLARNDVGRVSDYGTVKVKSLMAIKRFSHVQHIVSHVVGHLSDNFDVFDALKALF
                     PAGTVSGAPKVRAMEIINELEKERREPYAGALGYFSFNGCCDFAITIRSIFIQGKDGY
                     VQSGAGIVMDSIPSNEWEETERKADAMISVLKNKNSDTEDKNKFPK"
     gene            complement(15828..17171)
                     /gene="thrC"
                     /locus_tag="VF222_04115"
     CDS             complement(15828..17171)
                     /gene="thrC"
                     /locus_tag="VF222_04115"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7141643.1"
                     /translation="MNHAVKKCINPSCGLIYEIIYNNYQCSCGSFLDILYSSRPPKEL
                     IEIFYQRRNPNGNIYNESGVWRFRELLNFPEIDTENLNECSNVLVSLDGAEGRLSKPY
                     NMSKVADFVGLPNDNLFLQPEGYNPSGSFKDNGMSSAVTHAKMLKIKALICASTGNTS
                     ASAAMYASNENMECDVFIPKGEIAPGKLGQAFQFGAQVIQVEGNFDDALQSSLEYSKT
                     SRGYTVNSINPFRIEGQKTIIYRALEHLEWNTPDWIVYPGGALGNTSSCGKALTELHE
                     WGWIKKIPRIAVVNAEGANTLYELVNGKFENTILHWNGGKPDINQIQRYYQYKKNNNI
                     LPKTRATAIQIGKPSNIVKGLRALWYTNGLVIQVKDREMMDGMSIVGLNGFDCELASG
                     AVPAGIKKLKEKDIIKKDDIVVGILTGRQKDPSLSMEYHMNTTNRFSKPPKNIYQ"
     gene            complement(17354..17641)
                     /locus_tag="VF222_04120"
     CDS             complement(17354..17641)
                     /locus_tag="VF222_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141644.1"
                     /translation="MLNIGNDLMEEKKIKVNNREYNVTLNDQTQLNAMKLKRLYQQSY
                     TDMDSFDEVSTEISSTINNLLKRSVSPDVLEEDMDGAIQQILKMYEKKGKK"
     gene            17945..18952
                     /locus_tag="VF222_04125"
     CDS             17945..18952
                     /locus_tag="VF222_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX7141645.1"
                     /translation="MKKDYSHYEIFYCNELYFMLKEIITKERARGLLLSGGLDSSIIA
                     SFQKPEYTFTVTLHGSNASDSQYARLIAKQYGSKHVAIKIKTLELLKIEEKVIELFRT
                     FDPIFIRNSSVIFAAVQKAKEVGVSAVLTGDGGDELFAGYNYLKKYFKDKPKLEEEVK
                     RLWNIMHFPSGTIGKFFNVKITSPYLDEKFMRFAKSINISMKMGQYQNKLWGKFILRK
                     CFEKWQHQEKHAWREKEAQEEGSGFSKIKDYFENQMFTNKKFLNEIKRIKDIESVKIR
                     SKEHLFYYLTYRKYFLPPRSQKELLELRICPDCSGSFRWMGKFCRICGAYPVNPIIQT
                     E"
     gene            19300..19968
                     /locus_tag="VF222_04130"
     CDS             19300..19968
                     /locus_tag="VF222_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HEX7141646.1"
                     /translation="MSFNKRWVKPTTNEGVANKLLENKFMDNIKSPVPLKPKIEDAQK
                     KLQLQISKLDGISTKMNEKDHLIFKRIVYAMQNHDSQYAKVLSNELSQIRKMNKMVSS
                     AKLAMEQIQLRLNTITELGDVVVTLTPAMSVIKGIQGGLSSMMPEADQSFGQISDLLS
                     NIMNDSSQIPNTEISGTGMISEDSMKILEDASMVVEQSMKDKFPDLPSSSEIDQESSE
                     ASIY"
     gene            complement(19987..20307)
                     /locus_tag="VF222_04135"
     CDS             complement(19987..20307)
                     /locus_tag="VF222_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141647.1"
                     /translation="MKIVEVKIKVSDESYQEFLSYLKSLPVDVILFGLRFAYKRYAAE
                     TGGYLFPGRKSIIKKETNMLSKEQAQWRLDNWKKMINNYREKGYSYPTISRIKKKIEN
                     IVTN"
     gene            complement(20557..21729)
                     /locus_tag="VF222_04140"
     CDS             complement(20557..21729)
                     /locus_tag="VF222_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7141648.1"
                     /translation="MASKYDHIKDQLQEYREKNIYRELLISQIKGKTFFLGKKKVINL
                     GSNDYLGLSQNRAVLSKIRNSLRQVSPCSSRLISGTNVNIIKLERILAKHRSAENALV
                     YPTGYMANLGVITAISSPEHTIFSDSLNHSSIIDACRLSKSNNIKIFQHNDPDHLERL
                     LKNSNTKKIIVTEGVFSMDGDIARLDLLSELAKKHDSILILDDAHSDFVFGNFGNYGG
                     IPEFFGVEDKVDIHISSLSKGLGCFGGYAASSNLITDLLINKSRQFIYTSAIPEHLCV
                     AALTAVSFLKKNKDLQRNFLKKIKWFINQLKQLGFNIGATSSQIIPIIIGDEKVASNF
                     SKELFKKGIFIQAIRYPTVSLGKARLRISLNTLIEQSQLTYVIICLDKLGKKYNII"
     gene            complement(21713..22723)
                     /gene="bioB"
                     /locus_tag="VF222_04145"
     CDS             complement(21713..22723)
                     /gene="bioB"
                     /locus_tag="VF222_04145"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX7141649.1"
                     /translation="MNSGDLISNNDSNNTEDFFIDILIDKIFNNQQVSFDEAYTLLST
                     DDISTLAKGANTITRKFNGKVVDIESLVNAKSGSCPEDCSFCAQSSFYNTEITKYPLL
                     PTEKLIELARNAKEGGSTNFCLVCAYRSPPEKDFELICDAILKIKNSIDIDINVSLGF
                     MTQERAKRLKSLGVKRYNHNLETAKSYFSQICTTHDYLDRVNTGKIVKEAGLELCCGG
                     IIGMGESPEQRLELAFALAELQPDEVPINILISRKGTPLMYNNPLTNEDIIKTIAVWR
                     FIMPKTILKIAGGREVYFGDNGRYALQAGANGIISGGYLTTGGNKQQKDIEMIKEIGL
                     EV"
     gene            23196..23828
                     /locus_tag="VF222_04150"
     CDS             23196..23828
                     /locus_tag="VF222_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7141650.1"
                     /translation="MQKYAIAYLRVSTEEQSVQTQKIAITKWAKDHDYQMLYFFEDPA
                     VSGKIPASSRKGFQDLMEFVRLSKVEAVLVYELSRVGRTFWDTLDAIKVIEEHSPLIS
                     CSPRESFLQTTDPSIRKLMISILTWVAEREREMLVQRTKDGMNRARIAGKEIGRPEKV
                     IDKNNLIALLAENISRAKIAKELRISKATLYKEFRRLNIHYNDSKNMFHQ"
     gene            complement(23806..>25019)
                     /locus_tag="VF222_04155"
     CDS             complement(23806..>25019)
                     /locus_tag="VF222_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7141651.1"
                     /translation="SVWGYSSNSVIEAIRRQFRELPHSTLFGLGNKPSIVLATKLLNL
                     TKGMDKIFYSDNGSTAMEVALKMAVQYWKNKQFPKKNRFLSLDNGYHGDTIATMSLGY
                     LDKYFNPYKSLLIRTLRAPAPSSFENNLDNVLTDSEIVDICTEKTEHILEKNSNQIAA
                     LVMESGAQIAGGVSIYPTSYQKRISDLCKKYNVLLILDEIATGFGRLGNMIEYLAQSS
                     KPDIVCYGKALNGGYFPIAVTLTTEEIYKAFLSEYNEYKQLFHGHTYTGHPSGCAACI
                     ANLEMYEKKNLIQKIRDNSRHIKKRLKELEDYKSVHKIRHKGLLIGVELAQETKVSQG
                     KKKKHPLSFIDGIHLNNYIMRESLNRGVFLRSLGNIVTLIPPLAMPRSQLDILIDTLN
                     DIIKSIDETYS"
BASE COUNT         8644 a         3928 c         3680 g         8767 t
ORIGIN      
        1 tggttgttgc tgttgttgac cttcatcttg taattcagag gatgttgtca tgcctggttc
       61 ttctgtcgta tctgttattg ctggctgtcc ttttattgta acatttcctt ctgttgcctc
      121 ttcttcttga gaatctgctg attttaaagg aatttcctca attgttatag ttttacctcc
      181 ttgtaatagt tcttcctcat tattttgttg attattattt gtaacatttc cttgtcttga
      241 taagtcatca gttattgctg gctgtccttc tgcttcagta acatttcctt gtcttgataa
      301 gtcatcagtt attgctggct gtccttctgc ttcagtagta ttagaaccaa ctttagtact
      361 attaataaaa tttagaatat ctcggtttgg taaatttgtc tcagagaata tattatcacc
      421 atcatcatgg tccgcagttg tattagagat aatttcagta tcattccctt ctaaggaagt
      481 ttcagaatct cgagacatat tttgagctat aaatatttgt cctaattgag tatcatcaaa
      541 tttgtttttt atctttgatg agttaaatga aataatatca ttatcgttcg aaatgatggt
      601 ctcatttttt attatttctt gtactagact cctaatagga tcagagaatg aagtttcgtt
      661 cggaataatt tttacttctc tgaattcagg atcattgata gtatcaaatg acaattcttg
      721 gatttctctt gcggttaatg gaggaagtct atctttatct atctcttcct cttcctcttc
      781 atcagtatcc tccaccccaa tggaaggacg atcttttatc gatatagcat cacttggagg
      841 agcttttggt gttctgtctg attcaggagg aggaataaaa ggtgcttcgt tacctttgaa
      901 agatttatca gaagtttgag cataagctat tgaaatagtc tgcatatcca ttaaggatat
      961 cgctaatgta ccaattaata ctgctataaa taagaatttc atcttcattt gtctaactga
     1021 tcaataacaa acatatcatt aatataaata atacttgaac aaagcgatta aatgtcttac
     1081 agaataaatc aaagtaaaaa atccaataaa aggttgaatt tctattaaaa tacttcacag
     1141 ttttttgatt tagtatctcc ttcaaattta tcaaaatcta atataatatc aaatccttgg
     1201 ccgcaatgaa actcatcagc tcccgttcca ccaatcataa catctttccc agattttccc
     1261 cagagaatat catcaccaga ccctcctatg agtaaatctc gtccttgtcc tccattgatc
     1321 caatcattac cgctattgcc ttctattcta tcattctgtt tttcgccata cagaatatca
     1381 tcatctttgt taccctgaat aatatcatca ccattaaatc ccaatatatt atctccgcca
     1441 gaatttcctt gaatcagatc attgccggta tatccgtcga tataatcact ccctttattt
     1501 ccttcaattc ggtcacttcc agaattacca tatagtttat ctttaccgct attgccttct
     1561 attttatctc gtccttcacc cccatcaata taatctgatg catctctgcc ccacaatttg
     1621 tccttttcct ctaatccaaa gaggctatcc tttttgttcg tcccttttat ttcatcttct
     1681 tcctctgttc caacaataat agccgcaaag acaggtatca aattatcagt attgattata
     1741 cccaaaataa gaagtgaaag gcccattacc aaaaaaacga tcaaattatt tctcatttat
     1801 taacatctaa aaatctactc aaaaatataa atatattctt ataaagatac aacaatttta
     1861 gccttaaaag actggattta tcaattcttt aatttccaat cgtgttagta tattagaaaa
     1921 agtacaacaa agagatattt tctaaactat acataaaaat aaaaggttat tgttgaaatg
     1981 gtttttatct gaatttaatt aatgttagtg acaatattga taagataaac aaaattttaa
     2041 ttcgtataac aactatatta cattatcttt taatttctat ttttttcttc aacatcaaga
     2101 ggtctgaggc ctctttaata gaaaaatttt catggactat cgattttaca gcttttatca
     2161 tagcaattgg agagtctgat tgccaaatgt ttcttcccat atcaacgcca gaagcgcctt
     2221 ctctaatggc attaaaagtt aattccaata catctatctc tttttctagt ttttttcctc
     2281 ctgcaataat cactggaacg ggacatcctt caattacttt ttcaaatttc tcacaataat
     2341 aggttttaac aagatgagca cccagttccc cagctattct acaagctaaa cctagatacc
     2401 gagcatctct taccatctct ttacctactg ctgttactgc tagaataggg ataccatacc
     2461 tttctccctg gtctactaaa gccgcaagat tagataatgt ttgatgttca tatttagaac
     2521 ctacgaaaat agataatgct agacacgata cattcagtct aattgcatct tccattgacg
     2581 taacaatagt ttcttttgat aagtcttctc caataatact ggtaccccct gatactctca
     2641 aaactatagg aacattggaa gtaggggtta cggaggttcg taaaactccc cttgttagca
     2701 tgattgaatc aacataagga agtaatggtt gtatggtttt ttgagggact tctaattttt
     2761 ctgttggacc taaaaaataa ccatgatcaa cagctagcat gacacaacgt ccatttttcg
     2821 gctttattat tcttgagaat ctgtttttca tgccccaatc cataatctat atcaacagaa
     2881 ccttggtaaa ttccttgatc tatttatttt tttttatctt tggtcctacg tagtaacaat
     2941 tactttcata caatctatag ctttatgagc acaaaaaact gcatcaggag attcctctag
     3001 ggaatatttg tgtgtaataa gaaaatctaa attaagtctt ccttctgcca gtaacttcaa
     3061 tgcttgatta gtttcaattt cagaagcagc ataacttggt actagtgaaa attcattaga
     3121 gtaaagttta ctagcttcca aagggaattc tgaatcttta gatggtactc caaacagaat
     3181 gatatagcca ccttttctgg tgatttcaaa ggcattagat aatgctgtta agctacttgt
     3241 cgcgactatt gatatgtctc ctcctctacc tttggtctta tctttaataa ttttgttgta
     3301 gttttcattt aatgaattta taaatattgc atctgaaacg aatttctgag aaaattctaa
     3361 tctaaaatca ttaatatcga ctacaaatat ctttccggct ccatacattc gagctaaatt
     3421 aagtagcata attcctgctg gtcctgctcc aaagatgacc aagtcatctc ctttttgaaa
     3481 gggtatttta ttaagagctc ttatacagca tgctaaaggc tcgattaaag aggcctgctc
     3541 ataagtgagg ttatctggca actttaacac accaccacgc tcaacattcc aagctggtac
     3601 aataaactta tctgccaaac cgcagggatc taaattgctt ttttgataaa attcacacat
     3661 ggtgttattt ccatgtctac agtaatgaca tgaattacaa cttacatgat gatgcacgaa
     3721 tactcgatct cctaccgtaa tgccctttac attatttcca attgataaga cttctcccgc
     3781 tggttcatgc cctagccttg ccgaagtcat gccatattga ccatatacct tttctaaatc
     3841 ggaaccacaa ataccacaag ccttcatttc aacaagaaga tcaccgtccg atttgaactc
     3901 gagtacgtta tcgtctaact cgtaaacttg aactactcct tgcgatacta tttttacaga
     3961 cttcacagaa acattatcta tagtggatta taaaatatat taactattgt gatatttttt
     4021 tggttttctt ttgaaaatac ccttacatcc ttcacaacaa aaataatatt ttttgtcatt
     4081 gtactctaat gaaatggcta attcttcctc catttctatt ccacaaacag gatcaactgg
     4141 cattctataa aaacaaatat tttttataat tatttattta tttctatatg taaataatat
     4201 ggtgactata gtattttctc aatattattc agtatcatat cctttttttt caactattat
     4261 actcccaaat tattatcaaa tattcaattg ttataataat tatttaatct gaatttttgt
     4321 tttagacaaa attaatttta tcgacttaat tatgatagag ttttattttt actcctacca
     4381 tccaaggggt tagttatcag caggttggac gaatgtctca gatagggaat tataaacgat
     4441 ttaagaaaaa cttctttcat tggatgctct cttaatcgtc ttagtatata ttatccatag
     4501 aaaaatttct ttcttctatc tttctctaat tgaaagattg ctttgctgta atctagatgt
     4561 ccaaagttac aatgtataaa gaaagaacac tgactgcaat gtttggcatc tatggagatc
     4621 caaaaaatac aatgcgaaga atagatctaa tacaggttaa attttaaagt taatgttcag
     4681 cttaagacag ttatcaacaa ttaatggaat ctatccaagt agacaatgct ctaagttcaa
     4741 ctgtattaag aataataact gctggaaata gctgtggcct aaaattaagg tattaactat
     4801 taagtaaata aaaggtatac gataatttct tatcagagat tagataaagc agctcatact
     4861 gataatgtag aacgagacga cgagatttta gaagatatat tcatttgttt ttaagaatgt
     4921 atattcccaa tgttagtccc atctctgtct tctttaaatg atttatctct accattgaat
     4981 tcataatagc aaataaatgg agattaatct aaattatttt agttaatgta ctcaaattaa
     5041 aagggtttct gtacttttgt ttctaatcat ggatcttttt tgtgtttttt cgttaaaaga
     5101 taaagttttc cacaaacatt aattttgtca aaattgtaaa gaaattttta caagaaatat
     5161 attattctac tatagaattg atattggtaa tgtcttatcc ttgtttagaa gtagtatgtg
     5221 gtaattgcgg atccacaatt aatcgaatga taaatctcaa atcaataaga gatagtatgc
     5281 gtccttctag tggaagatgc aataaatgcg gaatttcact gaatccctca gatttctcat
     5341 tgaatactgt aaaacaataa cattttatca tggaatccta ctaatttatt tattcgagaa
     5401 attcactcaa ataaaaaaac ctatttattt ttatataata ccatttttag gaatatataa
     5461 ttattctatt tattaattat tgcaattatg taactaaaaa taagacaaat acctattgtt
     5521 ctagagaatg taattgtgta ggatattgct ttaaccaaat cttctatttt gtcataattt
     5581 ttcaatagtt gtgaagacct gatagcaaaa agcaatgaac taaaagttat aattgagtaa
     5641 agcggtaaat attgtataat tattccaatt gctattaaag aatatatcaa gctaataaga
     5701 agcggaaata tttttgatgc tttatctttt cgcagtaaga taactaacgt ttttcttcct
     5761 ttttctttgt cggcatcaaa atcggggaag gatgcgatat ataaaacaat agaagataat
     5821 gctccaatta ttattcctaa taaaacgctt ggtaaatcga taataccaga ctgaacgtaa
     5881 gaggtaccaa taacaatcat tgaaccttta actgcaacaa gtgtttctcc taaaccaata
     5941 tttacaatag tagtagaata aaaataaatt gatatagtag cgacagctaa aataattgca
     6001 attataaatc cactatgaaa aacaaagtat cctcctaccg ataaccctat caccatacaa
     6061 ataaatcctg ctttgtatac tgtagatgga tctagttttt tttcaggaag tactccagtc
     6121 cctccactaa aggtagttct tttagtggtc gtatcaattc ctcttttata atcccaataa
     6181 tcattaaaaa gatctacact agcatgtaaa aaaataacgc caatatacgt tagcaaagca
     6241 tcaattatat taaattgatt gtaagtaaag taggtatata ataaaccatt agtaatagct
     6301 ataattgacg ccagtaggaa tttaattctt ataattctca gccagattga aatattcaaa
     6361 acaacattaa gtttgctatt aattctttat attttatagt aatttcttca cattaaacat
     6421 aaattaagga atttacccaa tacgagattg aaaactattt gtgagaatcc ttgatgtaaa
     6481 ataatatata taacttccaa agtaacatga ataataacct ttaatcattt tctttcatta
     6541 ttatttcact ttcggtgata aatttaaaaa atagtgaaaa tacataaatt atcttatgtg
     6601 gatctcacac attattgatg tctatactct ttacagtaga taaagcaaat actattttac
     6661 caaaagtgaa aaagaggttt gatgaaatat tatgctgtaa aaataatgta atggatattc
     6721 aagaagagct acagaatctc tccgattcta attgttcatt tgaaaaattc ataaagaaaa
     6781 aacaagaact taatcatgct gttacttctc tatacaacat gatccaagaa ttagaagatt
     6841 tgggaataat gataaaaagt gttgatgaag gtttactaga ctttccatca atccgggatg
     6901 atgaagaaat atggttatgt tggaaatttg gagaaaatca agtaaaattt tggcatagaa
     6961 aggaagaagg ttttatgggt agaaaaccta ttccacaaga aggcttcgaa tatgttgatg
     7021 attttgctga cttgagatag ttagatacta aacacctata tgatgatctt cgaaatgcac
     7081 cactgaaatc taaatgatca ttgttatggt ctttgagatt agaggttatt attagtaatc
     7141 tgtggaaaat cggaaatcgc attagttaga aatgatagca agctattata tttcaataat
     7201 aaaattgtga tttaaacatc taataaatgc catgttattt taaattatat attcgagtac
     7261 ctacctatat caaatacagt atgataaaat atatcgacta gtcttcatta agaaatgaag
     7321 atcattcaaa atgaaagaaa tttacttcaa catcattctc gcttcagctc ctttaatatt
     7381 tcgatatcag cgttgaaaaa ggccatcaaa tctgttgatc cgtgtgtatt aataaaaaga
     7441 gctattgata tcaaagaaaa taaactagta gttgttaggg acgtaaatga tgttaactat
     7501 ttttttgatt tcaaagacta cgagtctttt catattgtgg gtgcaggaaa agcaacagca
     7561 agaatggcta aatctttaat ggaaacgtta aatatagaaa tttcctctgg agccataact
     7621 ataccctata atgaaaagat attgcctgat aaacttatag taacagaagc tggtcatcca
     7681 gttccagata aaaatagcat acgtggaacc aaacagatga ttgatctact gaaaaaaatt
     7741 gaaactaaat ctttagtatt tgtgcttata tctggcggat cctcttcatt aatgtgttat
     7801 cctttagatg gattagaact taaatctaaa caagtactaa caaagctgtt actaaagtct
     7861 ggcgcatcga taaatgaaat taatacagtt aggaaacatc tatcaaaaat caaaggaggc
     7921 agacttccaa aatatgtgaa tgagaaagcg agaataataa ctctcatttt atcagatgtt
     7981 gtaggtgatg acttaagtat tataggatca ggcccaacag ttggagataa ttcgacattt
     8041 gcaaattgta tagatatatt aaaaaaatat gatatatgga atacaaacaa caatgaaaat
     8101 cccctagaaa tttcaaatgt gaagaaagta ttggaagatg gaaaaaaagg gattattgaa
     8161 gagacacctc cccttcatca tccgatttac gagagagtaa gtaatatttt gattggaaat
     8221 aatgaagtag cgtgtaaaag tgctatgaat gaattactca aatttgaaat aaaaacaaac
     8281 tatataggat cccacatcaa ttctccagct aaggtttttg gaaaagaatt agcacaattg
     8341 ctcaagtcac ctcaaacaaa accctttaac cataaagagg aaaatgggtc ttttgcatac
     8401 gtcatgggag gagagactgt agtaaacctg acctcaacta acaattcgaa caataacgtt
     8461 gagggaaaag gaggaagaaa tcaagaagca ataatttcag ctatcaatta tcttcacaac
     8521 attaaaaatc ttgacaattt cactataatc tgtgctggaa cagatggaat agatggcaat
     8581 tctgaatctg cgggaggttt agtatctcct tcaacactag aatctataaa gaaagcaaaa
     8641 attaatgtaa atttctactt gaattctcat gacagtgaaa ctttactaaa aaaagttaat
     8701 tccaatataa ttactggcag aactggaaca aatgttaatg atattgctat catttgtaaa
     8761 atagaataag gatttagata tacttaataa tgaagtattt ctatcgagaa aaagggttga
     8821 atcatataaa taatttaatg atagaatata attactacat ttttataaac ctttaagtcg
     8881 gaatagacta tcatcggaga acttaatccc ataataaata ataaaattta aatttccttt
     8941 tgtcattttt tttctctgat agaaatttct ccggaatata ttttctctaa tgaattatcc
     9001 tcttttgcta gaattaatcc accatcggta tcgatatcct ttacaattgc gctatatttt
     9061 ttcaaatcat cttgataaac aattattctt tttcctagtg tattaataag atgtttgtaa
     9121 atgtcaataa ttcttgtatg atacttatcg tactctaaca aggacaaata aaattctatt
     9181 tttactaata attgtttcag taaagtgaaa cggtcaattc cttttccttt aatttcattt
     9241 tgtatagatg ttactttcca agacattata ctgttagagg cattagataa attaattttt
     9301 gctatatcaa tatcgatatt aattcctatt ccaattacca cgtagtcctt atcactatta
     9361 ttggtactag aatttattaa aattcctgca attttttcgt cgtttattaa aacatcatta
     9421 ggccatttta cataggtctt taacttggaa atttcattga ttgcttcaca aacagaaatt
     9481 gctgagatta ccgacatcat attaatttta gtaaattcaa ttctaggttt aataattaag
     9541 gacatccata accctccgat cggagaaatc catttttttc tatttcgtcc taatccttca
     9601 tcttgttcat cagctacgat gacagttccg tgttcattct taatattggt ttcagctagt
     9661 gaaatagcga gttgctgggt tgaccttaat tttttataat gataaatgcg tttaccaata
     9721 acgtgatttt gtagtcctaa atccattttt ttatatgggc ttcccatact ctgtctattg
     9781 aaaaaccaca ggatatagca ttttttaata gagttagcga agcacaataa ataaaactta
     9841 taataaatac atcaatatct gttatttaat aatctatcat tgatcactta caccaatttt
     9901 tcttacaagt tacgccgata tttgaaacga aatgtaatat tatcttgatt tcattatgat
     9961 tttcattcta gtaattggac taaaaaaaat tgaatacttg aaatcacaac agaaagttgg
    10021 ctaatccatt atattcagat acgagattcg aagataacgt ccacatttgt ttgattttgg
    10081 atttattttt ttcattaaat gagtcctcaa atgcgtatgc agactcaata ttgcacttta
    10141 ccatagggaa tcaatccata actatttaat catgtatttc cttagaattt tagcaaaaag
    10201 tccaaacaaa ctcaccatgt ttgatagctt aaaataatat atgatttaca agaatcctga
    10261 aacaaactga cttttattat tgctatttaa tggaagatat ctcattttta cttgaagaca
    10321 aattttatac ctgtaattgt tggtgaataa ctagaaacgg ttactttttt ctgaacactt
    10381 tttttgaatg agccacgcat agtgaggtac taaaagaatt attccaaaat tggagaaaaa
    10441 ttacaagttc aactaatttt gataaagcta catcttgcgc agtaactaag aaaataaaga
    10501 gtttttcact gattacggat aaattaatta atcttaaaaa tgtaatagaa aatccatatc
    10561 ttggagaatt attgctatag tcaaagaaaa tctctgggga gtttggaata gagatgatat
    10621 ataaatcttt gaaactattt atgaagaaga taatttagga gaatttaatt attatgttct
    10681 tttcttatta tcaagaaaat ttctctatta ttaattactt attatcgaaa aaaaaatttc
    10741 atatgatgac taaagtaaaa caaaattaag gttttaatta aaatttaaag aagcttagtg
    10801 aaatagcatt atattgttat tccaaaatga ttctcaaaaa tagtataaca taaaataata
    10861 aaggaaaatt taaatcaaat taggatgggc attacgttta ttttttactt gatcggtaat
    10921 attttctatc tggtttaaat tttttacaag tacaattttc tattagacat ttcattttat
    10981 caattttagc atgtttatca tgatcaattt tttggtgttt gcaccttaaa catttaacaa
    11041 atgtatctga atccatttcc aaattaaagc atcatcattc aacaatagag aacaataact
    11101 aatatatttc tataaagaga atctcataat tgttcttttc attttatcac tatgtatgac
    11161 agccttcacc aattaattga aataatatga ctctttaaaa tatacataat tcaatttacc
    11221 attaggaaga tgcatgatca agctgtaaaa tccaatgaaa aagtaagcct aacgttctta
    11281 taataaacag ccattaataa gttatgagaa aagtaataga aactataaga aaatcaaaca
    11341 ctgcaaaaaa tagcgatgag cgcgttataa ttgataataa taacaataaa catttagagt
    11401 tttttatgaa tggtagttgg aggcatagtc acaaatgtaa aactgtagta aataaccaat
    11461 gtatcaagaa ataacttctc ttcattaatt aattattccg ctattatgat aaacaatcaa
    11521 attttgtagt attggtagac tcacattttt attagtgttt ttggtaaagt atgcctcaat
    11581 tttatagagg tttgcaaaag atttccatca cggaactata gatcagttat cacttttttt
    11641 ctatctttct tattataata aaaacaaatt ccacccgata aaaatcaggt tgttccatgc
    11701 aaatacaaat aacataatta tttttaataa atttcattta cttgcgaata ttttaggaat
    11761 ttatcagttt taacaaattt aggagattta ttgaatcgtt cgacaaaaaa attatttgac
    11821 taaaaaaatg cattcatata tattatctta aatttcactt aactatatga gtaatactag
    11881 taatgaacaa tacggggata ataatgatca acgaaatttt actaataaac cggtagaatt
    11941 aggaaaggaa tacaacgttc aaataacaga aacgagttat aagggtgacg gcgttgcaag
    12001 agttcaaggt tttgtgatct ttgttaaaga tggtaaagtc ggaaatgatg tcaaaattaa
    12061 aattaccagc attggtaatc gattcgcaaa agccgatctt gtttaaagga aatccttagg
    12121 atttttaaaa ctactgtttt taaccgcatt ggatataata ccttaaaaaa tttaactttc
    12181 ttatttatct gttaattttc attacccgtt atgatgaaat tgctttctct attttcaact
    12241 cactttttca tcggttcttt acttattaaa gaagaaatta ggctgtcttc cactgataaa
    12301 atggtttgtc ctattaaatt gaccaagata accattaaaa taccaagata ctctcaatag
    12361 ttaagctgat tatttgatat ctccaattaa taatcgtatg gaatattgac ccgaaaaaaa
    12421 caataaagaa aatttcgttt tgatattaat tttaaccgtc aggctaatgt gaaaaatttt
    12481 ataaattaaa caatattgct atgaggaatt atttaatggt aatttgttag tttaatgttt
    12541 tccttattat tttgggacaa tgaatgatat gataatattt acacaaactt ctcaatatta
    12601 agactacgaa ttggtgtgat aatgcgataa agattactaa ataaatcaaa attttcccaa
    12661 tgaccaagat tcctacgaaa taagctaatg caattaataa tattggtttt agtataacaa
    12721 attaaaaggt aataaatcta tttccttatc gaaacattag ttattagatt ttgaattatc
    12781 ttttcattaa actgtacttt gtattttcta ctcctcggtc tagtgcgtaa acgatatcca
    12841 caacaaggac accataaacc ttcccatttt ataaaaattt gacatacttg acaacgtttt
    12901 tgtccgatta tgtaacgttg tccactttta ggttttaatg ctttatgacg tgcacatatt
    12961 cccttgcatg gcattttaag ttggttccat taattttttc tttgtttgat tttctatttt
    13021 aaacaagtta tgcttaattt atgacattat atcttattaa atctagctgc atttcaatta
    13081 atggattaga ttaagtttta ttatataaga atattagcac gtcccaaatg aagccaaaaa
    13141 ttggtaaata ttgttcagaa taaacagcgt aacaggaggt tatgtattta aatgcaattc
    13201 gctaagtgaa taataaggat ggttacttcc aaacctccac gacaaataac tcaaggagat
    13261 gaatattcaa atatattcat ataagaagat tccaatatat atttaaatta cactgaaata
    13321 tagatagaaa taaaaaattc gttattgtcc agagtctaca ctagaatact ttccaaaaaa
    13381 aatgaagata gagacattct agataaataa gatatttttg ttattggaga attgatttat
    13441 tgttgtgaag ctttagaaaa gaatttgtga ttagaattaa tcgaacctaa aaataataaa
    13501 aatggattaa acgagccaaa aatagtatta ggtcttgcta actatagtgg aggttacagc
    13561 gtaataggag gtagggacgg aataaaattt ttgcccattt tgtggcagga aaatcgagtt
    13621 agaaaaagta ggctgtgaga cttaataaat acttaataca agtaataaat ttaaagtatt
    13681 aataatatga aacaagttta tttggtgact gttcagatag aaggaatggt tagccagtta
    13741 aagaatatcg tatttgaaga tgaatcagca tgtgataaat tcattgataa attgaaatca
    13801 caatcaccaa gcaaaaatcg atatttatgt tataaatgga aaataccatt aattacaaat
    13861 gaagattata ataaccttaa tgaaaatgaa gttttagaaa attccaaaga attaatataa
    13921 tttagtctga cccttagtaa acattaataa ttattgaaat atttcaagta attaggcata
    13981 acttgtatct gtagatgaga aaatatatta cctcaccttg tgttctaaat attaaatgaa
    14041 tatggattat aaagtaacct aaagaaggat aaaaattgaa gatgcttgtc atttaattaa
    14101 ttgccatcaa aatctatgac tttggattat tcattctaaa aacctaaatt tcacatatta
    14161 atggaaattg aattgaggaa tagttttaga gtaaaatttt tgtaatttga atcacataga
    14221 tttaaataat tttaaaaaat atttcagtta ataagaggtc attttcagat tgaatactcc
    14281 attatctatt tcgcagctaa agagattaaa tcattttcat atacaaaaac taaatacaaa
    14341 gaaaaaccag tttcaaattt attcagacat ctttaatttt tatgattcta cttttatttt
    14401 tgaatctcta tcaggtccta aagagttggc agagacctct attattggat ttagtccgag
    14461 tttcaccata aaatgtaatt caaaaaactt gattatcttt aataaaaata aaaaaaaagt
    14521 aaaaacacta aaggtgaaag aaccattatc tcaattaaga tctatacttc caaaaataac
    14581 tttacatgat tttaattata gatatattgg aggagcggta ggttatatta gttatgaggc
    14641 tatcagcttt tgggaaagat tacccgctcc gaagaaaaaa atactaaact ttcccttgtt
    14701 tgagtttgga atttacacag atggaatact ctatgaccat aaggaaaacc aagcatatta
    14761 ttttcataca gggctcgaat cccaaataga tgaaataaat aaaattttaa acaatgattg
    14821 tcatcctttt aatgctaata cggagttgaa ttggaagaat agacatcata ataaaaatct
    14881 taagttgaaa aacattaaat gttcagttcc acaaagaaac ctaagtaaag cagaattcat
    14941 aaaaatggta aaaaaggcgc aaagctatat ttatgatggt gatatctatc aagtagtctt
    15001 atcaaaaaac ttcaaattca ctatagaagg caacctactt caagtttatt catccttacg
    15061 aaaaataaat ccatccccct atatgtatct ctttaagatt gacaaaagat gtataatagg
    15121 atccagtcca gaaatgcttc ttagagttac tggaaaagta attgagacct ttccgatagc
    15181 tggaacgaga cccattggtg aaaatgagga agaaaccaca aaacttggcg agaatttatt
    15241 aaaagatgaa aaagaattag ctgaacatac aatgttagtt gaccttgcaa gaaatgatgt
    15301 aggtcgtgtt tctgattacg gtacggtgaa ggtgaaatca ttgatggcaa ttaaacgttt
    15361 tagccatgtg caacacattg tgtcacatgt agttggacac ttatcagata actttgatgt
    15421 ttttgatgct ttaaaagcac tatttcctgc ggggacggtt tcaggagcac ccaaagttag
    15481 ggctatggag attataaatg aattggaaaa agaaaggagg gaaccttatg ctggggctct
    15541 cggttacttc tcattcaacg gatgttgtga ttttgctata acaataagat caattttcat
    15601 acagggaaaa gatggatatg tacaatctgg tgctggaata gtaatggatt cgattccctc
    15661 gaatgaatgg gaggaaacag aaagaaaagc agacgctatg atatccgttt taaaaaataa
    15721 aaattcagat acagaagata aaaataagtt tccaaaataa tacgaaaaga gtttaaaata
    15781 aacattagtt ctatgttatt taatgtcttt ccaaaactaa tggtggatta ttgatagata
    15841 ttcttgggag gcttagaaaa tctgtttgta gtattcatat gatattccat agatagtgag
    15901 ggatcttttt gtcttcctgt caaaatccca acaacaatat cgtctttttt aatgatatct
    15961 ttttctttca atttctttat tcctgccgga actgctccgg acgcaagttc acagtcaaat
    16021 ccattgagtc ctacaattga cattccatcc atcatttcac gatctttcac ctgaattact
    16081 aatccattag tataccataa ggctctcaat cctttaacaa tgtttgaagg tttaccaatt
    16141 tgaattgcag tggccctagt tttaggaagt atattattgt tcttcttata ctgataatat
    16201 ctttgaattt gattaatatc aggtttacct ccattccagt gtaatattgt attttcaaac
    16261 ttaccattaa ccagttcata tagagtattg gcaccttctg catttacaac tgcaatccta
    16321 gggattttct tgatccatcc ccattcatgt aattccgtca gtgcttttcc acaactagat
    16381 gtatttccta atgcaccacc tggataaacg atccaatcgg gagtgttcca ttctaaatgc
    16441 tctagtgctc tataaataat cgttttctgg ccttctattc taaatggatt gatagagttt
    16501 acagtataac cccttgaagt tttggaatat tctaaagaag attgtagggc atcatcaaaa
    16561 tttccctcta cttgaataac ttgagcccca aattgaaatg cttgtcctaa tttaccagga
    16621 gctatttctc cttttggaat aaaaacatca cattccatgt tttcattgga agcatacatt
    16681 gctgctgatg cagatgtatt cccagtagat gcacagatca atgcttttat tttcagcatc
    16741 tttgcatgag tgactgcaga tgacatacca ttgtctttga aagacccact aggattgtag
    16801 ccctcaggtt gcagaaaaag attatcgtta ggaaggccta caaaatctgc aactttggac
    16861 atattatagg gtttagataa tcttccttct gcaccatcga gggaaactag aacgtttgaa
    16921 cattcattga gattctcagt atctatctca ggaaagttta ataattctct aaacctccat
    16981 acgccacttt cattataaat attcccatta ggatttctcc tttggtaaaa tatttcgatt
    17041 aattccttag gaggtcgtga ggaataaaga atatctagaa atgaaccaca agaacattgg
    17101 taattattat aaattatttc atatattaac ccacaagacg gatttataca cttttttacc
    17161 gcatgattca ttcgaatgaa tcattagtat tatcttattt atattattgg tgtctgttct
    17221 ttgccaattc attccttttc aatttttttc gctctaatta ggtaatcaaa tcaggaaata
    17281 taagtaatcg aaagcataat taaaatgttg taaaaatcta aaaaattatt attattttct
    17341 tttgaatgat atcttacttc tttccttttt tttcatacat tttgaggatt tgttgaatag
    17401 ctccatccat atcctcttca aggacatcag gagagacaga cctctttaag aggttattaa
    17461 tggtgcttga tatttcagta ctgacctcat caaaactgtc catatcggta tagctttgct
    17521 gatataacct tttaagtttc atagcattta gttgagtttg atcattaagc gtaacattat
    17581 actctcgatt atttactttt atcttctttt cttccataag atcattccct atgttaagca
    17641 tttaaaaaac tttcgatgta aatttcaaaa tcttttaggt tatcttaaaa atataataat
    17701 aataatattt tgaaatgaga aaaggattat taccaaagaa taaatacatt atattttcca
    17761 aggctttagg aaatgtattg attgaaagta aaaaaaacat attctacctt tatattgttg
    17821 atttcatgct ataaatctaa cagattgaat agttattctt aaatgatcgt gcgcttatgc
    17881 attactcacg atagtaagaa gattataata tttatcttcc tttcacctct atatattttc
    17941 ataaatgaaa aaagattatt cacattatga aattttctat tgtaatgaac tttattttat
    18001 gctaaaagag atcataacaa aagagagagc tcgtggcctt ctactatcag gaggtttaga
    18061 tagtagcatc atagctagtt ttcaaaaacc tgaatatacc tttactgtta ctctccatgg
    18121 ttccaatgcc tcagactctc aatatgccag attgattgca aaacagtatg gctcgaaaca
    18181 cgtcgcaata aaaataaaaa ctttggaatt attaaagatt gaagaaaaag taatagaatt
    18241 atttcggact tttgatccca tttttattcg taactctagc gttattttcg ctgcagtaca
    18301 aaaagctaaa gaagtaggag tttcggctgt actgacagga gatggaggag acgaattatt
    18361 tgcaggatat aattatctga aaaaatactt caaagataag ccaaagttag aagaagaagt
    18421 caaaagatta tggaacataa tgcattttcc ttcaggaaca attggtaaat ttttcaatgt
    18481 taagataaca tctccgtatc ttgatgaaaa atttatgaga tttgcaaagt ctataaatat
    18541 atcaatgaaa atgggtcagt atcagaataa attatggggc aagtttatct tgagaaagtg
    18601 ttttgaaaaa tggcaacatc aagaaaagca tgcttggaga gaaaaagagg ctcaagaaga
    18661 aggttcagga ttttcaaaaa ttaaagacta ttttgaaaac caaatgttta ctaataaaaa
    18721 atttttaaat gaaattaaaa gaataaagga cattgaaagt gttaaaattc gcagcaagga
    18781 gcatctattt tattacctga cgtacaggaa atattttctg ccaccacgat cacaaaaaga
    18841 gttattggaa ctaagaatat gtcctgattg tagtggtagt tttagatgga tggggaaatt
    18901 ttgtcgaata tgtggtgcat atccggtcaa tcccattatt caaacggaat aaaagtatgt
    18961 agatatattt gataatacaa gatatttctc atacctgatt tttattgacg tttgaatatc
    19021 aataaaatta gcatatcttc aattatttga agatattatc tttatttcaa atacaaatac
    19081 tctctctaat tataaaatat agtttatttt taactgtcaa gtctctcgtt tcaactcgaa
    19141 atcccaaaat atatagaata atgagtaagt taattatcac agatctctcc acagctgaat
    19201 atagttacct tttgaaatgt atctctagtg acaggaatca tagttacaat ccatttttta
    19261 aacaaatttg ttacatagtt ttcggtgtat agatagccga tgtcatttaa caaaaggtgg
    19321 gtaaaaccaa caactaatga aggtgtagca aataaacttc tagagaataa atttatggat
    19381 aatataaagt ccccggttcc attaaagccc aaaattgaag atgctcaaaa aaaattacag
    19441 cttcaaatat ctaaattaga tgggatttct actaaaatga atgaaaaaga ccatttgatt
    19501 tttaaaagaa ttgtttatgc aatgcaaaat catgatagcc aatatgcaaa agtattatca
    19561 aatgagcttt cgcaaattag aaaaatgaat aagatggtga gttccgcaaa acttgcaatg
    19621 gagcaaatcc aattaagatt aaataccatt actgaattag gagatgtagt tgtcacattg
    19681 actcctgcaa tgtctgtcat aaaaggtatt caaggaggtt tatcatctat gatgccagaa
    19741 gctgaccaat cgtttggaca aatatctgat ctattgtcaa atataatgaa tgattcttca
    19801 caaataccaa atacagaaat atctggaacg ggtatgatct ctgaagactc tatgaaaata
    19861 ttagaagatg ctagcatggt cgtagaacaa agtatgaagg acaaattccc tgatttacct
    19921 tcctcatccg aaatagatca agaaagttca gaagcctcca tatattgaac attagaaata
    19981 ttacctctaa ttggtaacaa tattttctat tttctttttt attcttgaaa ttgtaggata
    20041 actatatcct ttctcccggt aattattaat catttttttc cagttgtcta atctccattg
    20101 cgcttgttcc ttacttaaca tattagtctc tttttttatt atgctttttc taccaggaaa
    20161 taagtatcct ccagtttcag ccgcgtatct cttatacgca aatcttaaac caaataatat
    20221 cacatctaca ggaagcgact ttaaatagga taaaaattct tggtatgatt cgtcagatac
    20281 tttaatcttt acttctacta ttttcatagt tcttttttga aatttatctt tttatttctt
    20341 tctagataac ataggtaatc agtttatcta gctaggttta atcaaataca gtaaagatat
    20401 ttttttatat tattgcattt aaaattttgt tatatcttta tttttgtatt cgtctttaat
    20461 gattttcacg tgtcagattc taattcttat gctatacgaa cttgattatt atgaaaaaat
    20521 tcataataat atgtataata tatgactagt aacatattat attatattat atttcttgcc
    20581 taatttgtct agacaaataa taacataagt taattgtgac tgttctatta atgtattaag
    20641 agatatcctt aagcgagctt ttcctaacga taccgtagga tatctaattg cttggataaa
    20701 tatacctttt ttgaataact cttttgagaa attggaagcg actttttcat cacctataat
    20761 aataggtatt atctgactcg aagtcgcacc tatattgaac cctagttgtt ttaattgatt
    20821 tataaaccac ttaatttttt ttaaaaaatt tctttgaaga tctttatttt tttttaaaaa
    20881 tgaaacggca gttagtgcgg ccacacacaa atgttctgga attgcagatg tataaataaa
    20941 ttgtcttgat ttattaatta ataaatctgt aattagatta gaagaagctg catagccacc
    21001 aaaacatcct aagcccttac ttaaactact aatgtgaata tcaactttat cttctactcc
    21061 gaaaaactct ggaattccgc cataattacc aaaatttcca aatacaaaat cagaatgtgc
    21121 atcatctagt atcaaaattg aatcatgttt cttagccaat tctgatagca gatcgagtct
    21181 tgctatgtct ccatccatac taaaaacccc ttccgtgacg atgatctttt tggtatttga
    21241 attttttaac agtcgctcta aatggtctgg atcattatgt tggaaaatct taatattgtt
    21301 tgatttcgat aaacgacatg catcaattat actggaatgg tttaggctat cgctgaaaat
    21361 agtatgttcc gggctagata ttgcagtaat taccccaaga tttgccatgt atcctgtagg
    21421 atatactaga gcattttccg cacttcggtg tttcgctaag attctttcta attttatgat
    21481 attgacgtta gttcctgata ttaaccgtga actgcatgga gatacctgtc tgagggaatt
    21541 ccttatttta gataaaacag cgcgattttg agataaacct aagtaatcat tagaacctaa
    21601 attaatgact tttttttttc ctaaaaaaaa cgttttacct tttatttgtg atatcaatag
    21661 ttcccgatag atattttttt ctctatattc ttgtaattga tcttttatat ggtcatactt
    21721 cgaggccaat ttcttttatc atttctatat ctttctgttg tttatttcct cctgtggtaa
    21781 gatatcctcc acttattatt ccattagcgc ccgcttgaag tgcatatctt ccgttatctc
    21841 caaagtatac ctctcttcct cctgctattt ttaaaatggt ctttggcata ataaatctcc
    21901 atactgctat tgtttttata atatcttcat tggttaaagg attgttatac ataagtggtg
    21961 ttccttttcg agaaattaaa atattaatag gaacttcgtc gggctgaagc tctgctaagg
    22021 cgaaagccaa ttcgagcctt tgttccggac tttcacccat tcctattatt cctccacaac
    22081 aaagttcaag tccggcctct ttaacaattt ttccagtatt tactctatct agatagtcat
    22141 gagttgtgca tatttgagaa aaataacttt ttgctgtttc tagattatga ttataccttt
    22201 tcactcctaa actttttaat ctcttcgctc gttcttgagt cataaatccc aaagaaacgt
    22261 taatatcaat atctatacta ttttttatct ttaaaatggc atcacaaatt agttcgaaat
    22321 ctttttcagg aggagatcgg taagcgcaaa ccaagcaaaa gtttgtggaa cctccttctt
    22381 tagcatttct tgcgagttct attagttttt cagtaggaag caatggatac ttagttattt
    22441 cagtattata gaatgacgac tgcgcacaaa atgaacagtc ttcagggcaa ctaccagatt
    22501 tcgcattaac taacgattca atatcaacaa ctttaccatt aaatttcctg gtaatggtat
    22561 tggcaccctt ggctaaagtc gaaatatcat ctgttgacaa taaagtgtat gcttcatcaa
    22621 aagagacctg ttgattattg aaaattttat cgattagaat atcaataaaa aaatcctcgg
    22681 tattattaga atcgttatta ctaatcaagt cgccgctatt catatattct atattttcca
    22741 aaacctatac ttaatcgtta tttttaatat tacatttcta aacttgagtt tagaaacatt
    22801 tgcatccttt gaattgcttt cctgttaata ttggaattta ctcaggtctg tattctctat
    22861 agaatgggat aaatttttaa caattatcag gctgttgata ttatattttg attttctcga
    22921 ttaaatgcta cctcaccccg ttaacttcag ctaaaatctt aataatcata gcagaattat
    22981 aagaaaacaa cggttttcgt tgtttttatt ctattctaat gtagttcaaa aagggaccgc
    23041 tttgtgatac gtatagaaaa tagtatcttt tttttacctt taaaatatgc ttatatcatg
    23101 actaaatggt tcaaaaaata ttaccttttt tatactgttt gtcttttttt tttaacatta
    23161 cgatatagat atcattttaa tggttatttc tggatatgca aaagtacgct atagcatatc
    23221 ttagggtaag tactgaagag cagtcagtac agactcaaaa gattgctatt actaaatggg
    23281 ctaaggatca tgattatcaa atgttgtatt tttttgagga tcctgcagta agtggaaaaa
    23341 tacctgcctc ttctagaaaa gggtttcaag atttgatgga atttgtaaga ttatcaaaag
    23401 tggaagctgt tttggtctat gagctgtcaa gagtaggaag gacgttttgg gatacgcttg
    23461 acgcaataaa ggtcatcgaa gaacatagtc cactgatatc atgttctcct agagagtctt
    23521 ttttgcagac gaccgaccca agcattagaa aactgatgat aagtatacta acgtgggttg
    23581 cagaaagaga aagggagatg cttgttcaac gtacaaagga tgggatgaat agagcaagaa
    23641 ttgctggaaa ggagatagga agaccagaaa aggttattga caaaaacaat ttgattgcct
    23701 tattagctga aaatatttca agagcaaaaa tcgcaaagga actgaggata tcaaaggcta
    23761 cactttacaa agagttccgg agattgaaca tacattacaa tgattctaag aatatgtttc
    23821 atcaatagat tttattatat catttagagt atcaatcaaa atatctagtt gactccgtgg
    23881 catagcaagt ggaggaatca gtgtaacaat atttccaaga gatctaagga aaactcctct
    23941 attcagtgat tccctcatta tatagttatt aagatgaatt ccatcgataa aagacaatgg
    24001 gtgttttttt ttctttcctt gagacacttt agtttcttga gctaattcga cccctattaa
    24061 aagaccttta tgtctaattt tatggacaga tttataatct tcaagttctt tcaatctttt
    24121 tttaatatgc ctactgttgt ctcttatctt ttgaataaga tttttttttt catacatttc
    24181 cagattagcg atgcaagctg cacatccaga aggatggcca gtatatgtat gtccgtggaa
    24241 tagttgttta tattcattgt attcgcttag aaatgcttta taaatttctt ctgttgtcaa
    24301 cgttactgcg ataggaaaat atcctccatt caatgccttt ccataacaaa caatatctgg
    24361 tttagatgat tgtgcaagat attctatcat attacctaac ctgccaaaac ctgtggcaat
    24421 ttcatctagg attagtagaa cattatattt tttacagaga tcagagattc ttttttgata
    24481 tgaggtagga taaatagata ccccgcctgc tatttgtgcc ccactttcca tcacaagtgc
    24541 agctatttgg tttgaatttt tttcaagtat gtgttctgtt ttttctgtac atatgtcaac
    24601 tatctcagaa tctgtcagaa cgttgtctaa attgttttca aaagatgatg gtgcaggagc
    24661 tctaagtgtt ctaatcagta atgatttata aggattgaaa tacttatcga ggtaacccaa
    24721 agacatcgtc gcaattgtat ctccatgata accattgtca agggaaagaa atctgttctt
    24781 ttttggaaat tgtttatttt tccaatactg aacagccatc ttcaatgcta cctccatagc
    24841 agtcgaacca ttatctgaat aaaaaatttt gtccatcccc ttggtcaagt ttaaaagttt
    24901 agttgcaagt actattgagg gtttatttcc taatccaaat agagtagaat gaggaagttc
    24961 gcgaaactgt ctcctgatag cttcaataac agaatttgaa ctataacccc acacactac
//
LOCUS       DASVEV010000083         6584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_802782, whole genome shotgun sequence.
ACCESSION   DASVEV010000083 DASVEV010000000
VERSION     DASVEV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802782"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VF222_04160"
     CDS             <1..1558
                     /locus_tag="VF222_04160"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141652.1"
                     /translation="LSIIALVSTNQSLWITSPIHHFYIELFGAMLAGILSVYYISRAN
                     NLSDKFSLFIGIGFLVSTLIDLFHVIVSLLNMDDILFLKYFIPQTWFAGRLFLSVMLG
                     IAIVKYVSFSPVDSSTKQLQQQKSSNMNTNKSNENCKLSKLLLFYLIIVTLFVSIVAI
                     SSLFVVLPFSVIDDNIPIHRPYEIFPLALFVLSLYYFYKNGVYKNKDVFYTSLAIFIV
                     FGIFGQIIMLYSAQHFDTAHNMAHVLKDAGYFINIIGLALSSIQYNIRLRESNDRLRE
                     SNEMLTIQYEKVKESEKMKSEFINIAAHELRTPIQPILGLTDIIYSKIKDEELHELLD
                     IIMRNAKRLKRLTDNLLDITKIEGRSLMLNKEKFNLNILISEVLKDYVNKQKSQPKFE
                     IVYDFKHKEDTIVEADRDRLSQVIHNLLDNALKFTTHNQQMIFVIIDKKKEGKEIIVS
                     VKDTGEGISDKILSKLFTKFATSDPTTGTGLGLYICKNIIEAHGGRISAENNSDGKGA
                     TFRFTLPIQL"
     gene            2193..3167
                     /locus_tag="VF222_04165"
     CDS             2193..3167
                     /locus_tag="VF222_04165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7141653.1"
                     /translation="MSSAFIIPYYADGYEQEQGRKKLFIAETIEGLFSQTDEDWCAIV
                     VVDITPNERTHDYLIHLKQKYYPKIDVIFLERNVGAGVSRNVGVLKALERGHSIILFN
                     DSDDISHPKRLEVVKKILLENPHIDVVYSTFEVIDENNRLIPTEKISSPILEILESHL
                     QNPLEGDDIWIKMGTETGITNITSSTAVRIKFAYQCPFPNEKASEDFHSWMRMSAFGA
                     NFKYTSLIPTKYRIPSFIKYQASRARIGVNNFNQIKARVDSDGFSKATEIALARNIIK
                     PEEIPMLKEKFYKRLAKSMKREMENELAGELLNKAAQLEYESFVYLNK"
     gene            complement(3416..4441)
                     /locus_tag="VF222_04170"
     CDS             complement(3416..4441)
                     /locus_tag="VF222_04170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7141654.1"
                     /translation="MWVSKNVASKDHDVTLIASRGSVGEEGGGGRLPSNLNIIETIEP
                     SWEGTVAEEAHYLMYKELLEKEYGSGNGIVWDNTWHCFSYLSAKKFPKMKIIHTNHGT
                     VEWQKVPVKQLRFPRYIGLSRLHAKYMSSVLEIPVRYVHHGIPLPPIEETKNNNNNKN
                     GDYLLSINRIVREKGIEDSIDIAVGTRNAIKIAGDDIHVPDLSYVQMIREKCQNTDGL
                     AEYFGLVDNKTKTELIKGCKAVIACPKPTWIEAFGLYAVEANAYGKPVLALANGGLND
                     IIVNQTNGFLAKTPRELQGYVEKIYECSPESCRRRVEKLFTDEIMTKNYLRIFERLME
                     DEPAFRW"
     gene            4951..6201
                     /locus_tag="VF222_04175"
     CDS             4951..6201
                     /locus_tag="VF222_04175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7141655.1"
                     /translation="MKANVIHGDFNPCGGAERLSLTTMQALLEMGIDFDLTTLKSPDI
                     PKLENSYGKNLVSVMKSVHKINVINIMEELRQQQEEKDHEDYNYDITINTNGDAAPYY
                     HSSFSKDNAITYCHFPSTKYHIESENIDYLKTDLGMTERSNVFSNYKNSEEINNSKNC
                     KTKPQFSRRKEYFEILKYGYWNLMRNSTVITNSEFSRRAIVKAFGLDNIYILSPPIDI
                     ETFRNVALMANGDDDETNDIILVISRIAPHKKIENAIKLAKILKDNNVGKGMKIVGNL
                     YYYFFDYYSELKQMVLDLGLTDYITFEINASLDKLLSIIRESRVYFHPMIGEHFGMAV
                     LEAMAGGLIPVVPNEGGVIEFVPQEYQFNTIEHAAQIIIYVFNHLPKTERIKLSDDIN
                     KFSNSHYIEGFQTILNESLSRRTK"
     gene            6258..6434
                     /locus_tag="VF222_04180"
     CDS             6258..6434
                     /locus_tag="VF222_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141656.1"
                     /translation="MPNCEMCGNNSDKCYTVDLSITSDFDKDEQPFERNSTVCFECKN
                     KIITVFPKLEDEYF"
BASE COUNT         2302 a         1019 c         1079 g         2184 t
ORIGIN      
        1 tctttcaata attgctctag tttctactaa tcaatcttta tggataacat cacccataca
       61 tcatttctat attgaattat ttggtgcgat gcttgctgga atactctcag tctattatat
      121 ctcccgtgct aacaatttaa gtgacaaatt tagtctgttt attggtattg gatttcttgt
      181 cagtacttta attgatttgt tccatgttat agtttccctt cttaatatgg atgatatttt
      241 attcctaaaa tattttattc cacagacttg gtttgctgga agattatttc ttagtgttat
      301 gttaggtata gcaattgtta aatatgtttc tttttcacct gtagattcca gtacaaaaca
      361 attacaacaa caaaaatcct ctaacatgaa tactaacaag agtaatgaaa attgtaaatt
      421 atcaaaatta ttattgtttt atctaataat agttacttta tttgtttcta ttgtagctat
      481 aagctcgctc tttgttgttc ttccattttc tgtaattgat gataatatac ctatacatag
      541 accgtacgaa attttcccat tagcattatt tgtattgtca ctatattatt tctataaaaa
      601 tggagtctat aaaaataaag atgttttcta tacaagttta gctattttta ttgtatttgg
      661 tatttttggg caaataataa tgttatattc tgcacagcac tttgataccg cacataatat
      721 ggcgcatgtt ttaaaagatg caggttattt tatcaatatt attggccttg cattgtcaag
      781 catacaatat aatattagat taagagaaag caatgataga ttaagagaaa gcaatgaaat
      841 gcttactatt caatatgaga aagtgaagga atcagagaag atgaaatcag aatttatcaa
      901 tatagctgca catgaattga gaactccaat acagcctata cttgggttga cggatattat
      961 atattctaag attaaagatg aagagttaca tgaattatta gatattatta tgagaaatgc
     1021 aaaaaggcta aaacggctta ctgataattt gctggatatt acaaaaatag aaggtagatc
     1081 tttaatgctt aataaagaaa aatttaatct gaatattctg atatctgaag ttttaaaaga
     1141 ttatgtgaat aaacaaaaaa gccaaccaaa attcgaaata gtttatgatt tcaaacataa
     1201 agaggatact attgttgaag cagatagaga tagattatca caagttatcc acaatctttt
     1261 agataatgct ttaaaattta ctactcacaa ccaacaaatg atttttgtta ttatagataa
     1321 aaagaaagaa ggtaaagaaa ttattgtaag tgtgaaggat actggagaag ggatatcaga
     1381 caagatatta tctaaattat ttacaaaatt tgctacttct gatccaacta ctgggacggg
     1441 cttgggacta tatatttgta agaatattat agaagcacat ggcggtagaa tatcggctga
     1501 aaataattct gatggtaagg gggcgacctt tagatttaca ctgcccattc agttataagt
     1561 tacattaatc caaacaataa ccattcttct attctgttaa atacattact aagtatacaa
     1621 ggttcggaca tggataaatc gaggattttg taatgtcttt gtaataactt ctttacattg
     1681 gttctctaaa tgatcctctt taaattcatt attagatacg cataatcaaa aacaactttt
     1741 aacctttttg ggtattgtct tttagtttct ggcgctctca gcatataaaa ataattgata
     1801 gggatcatct cgactaaatt tgtcgttggt ataagtttgc tggctggttg ctcacgcatt
     1861 ctattttctt caatgtgtct atatacatgg gctgcattta agcgtcattg tcaaaacggg
     1921 ctcgcactga tattgttgta tcatggtagt tcatcttcat cttcataagc ttgacgcatt
     1981 ctaaatcgag tgccataatt aattagaaag ttatctcctt ggtactaaat ccatatacta
     2041 aaaaattatt atcgagtgaa aaaattagag aactagtatc tttatgtaac taaatctaaa
     2101 aacatcgaaa gcttaaatta aattaattaa ttggtagtct tgataacatg ccaaagagat
     2161 taaaatgata aaaaacaaac aatagagaag agttgtctag tgcatttatt attccgtatt
     2221 atgcagatgg atatgaacaa gaacaaggta gaaaaaagct atttattgct gagacaattg
     2281 aaggattatt ttcacaaact gatgaagatt ggtgtgcaat tgttgtagtt gatataactc
     2341 ctaatgaaag aacccatgat tatttaattc atttaaagca aaaatactat ccaaaaattg
     2401 atgtcatttt tctagaaaga aatgttggtg ctggagtaag cagaaatgta ggagtcctca
     2461 aagcattaga acgtggtcat tctataatac ttttcaatga ctctgatgat atatcccatc
     2521 caaaaaggtt agaagttgtt aaaaaaatat tattagagaa tcctcatatt gatgtagttt
     2581 attctacgtt tgaagtaatc gatgaaaata atagattaat acctaccgaa aaaatttctt
     2641 caccgatttt agaaatctta gaatctcatt tgcaaaatcc attagaaggt gacgatattt
     2701 ggataaaaat gggaacagaa actgggatta ctaatataac ctcttcaaca gcagtacgta
     2761 taaaatttgc atatcaatgt cctttcccta atgaaaaagc atcagaagat tttcattctt
     2821 ggatgagaat gtcagctttt ggagccaact ttaagtatac ttccttaatt cctactaaat
     2881 acagaatacc aagttttata aaatatcaag cttcaagggc aagaataggg gtaaataact
     2941 ttaatcaaat aaaagcaaga gtagatagcg atggtttttc taaagctact gaaatcgcgc
     3001 tggcaagaaa tatcataaag cctgaggaaa taccaatgct taaagaaaaa ttctataaaa
     3061 gattggccaa atctatgaaa cgagaaatgg aaaatgaact agctggtgag ctattaaata
     3121 aagccgcaca actggaatac gaaagctttg tttaccttaa caaatagctt gtgttgcaac
     3181 tatgactagg caatcatgga gaagatacaa acaatgtgaa tatgatattc tatattttct
     3241 aactagcata tgaatccgta tagttagcgg gctcggacgg ttgtgaatca aatttgacat
     3301 tattttgagg cccaaactga agttacgtac gtcacatatt ttcagttttt aaaatcaatt
     3361 tagaatgcgt aggatagtca agccataggg ttagtaaaac aactacctac ttttactacc
     3421 atcggaacgc aggctcatct tccattagcc tttcgaagat ccgaagataa tttttagtca
     3481 ttatttcatc tgtaaagagc ttttcaaccc tgcgtctaca agattctggc gaacattcgt
     3541 aaatcttctc gacataacct tgtaactctc gaggggtttt agctagaaaa ccattggttt
     3601 ggtttacaat tatatcgttt agtcctccat tagctagagc aagtaccggt ttaccatagg
     3661 cattcgcttc tacagcatat agtccaaacg cctcaatcca ggtaggtttc gggcatgcaa
     3721 taacagcttt acaccccttg attaattctg tctttgtttt attatctacg agtccaaagt
     3781 actctgccaa gccatcggta ttttggcatt tctctcttat catttgtaca tatgaaagat
     3841 ccggtacatg tatatcatca ccagctattt ttatagcgtt tcttgttccc acagctatat
     3901 ctattgaatc ttcgattcct ttttctctga ctattctatt tatcgagaga agataatctc
     3961 cgtttttatt gttattatta ttttttgttt cttctatcgg aggtagaggg attccatgat
     4021 ggacatatct aactggtatt tcaagaacgg acgacatata tttagcatga agcctcgata
     4081 gtccaatata cctaggaaat cgcagctgtt taacaggaac tttttgccac tctaccgtac
     4141 catgattggt atgaattatt ttcatcttag gaaatttctt tgcagagaga taggaaaagc
     4201 aatgccatgt attatcccat actattccgt tcccacttcc gtattctttt tccagtagtt
     4261 ccttgtacat gagataatgt gcttcttcag caaccgttcc ttcccaagaa ggttctatcg
     4321 tttcaattat gtttaagttg gatggcaatc gtcctcctcc tccttcttca cctaccgaac
     4381 ctctagaagc tatcaaagtc acatcatgat ctttactggc tacgttttta gaaacccaca
     4441 taacgacggc ctccaagcct ccattcgatc ttcaacaagt ctgagatgaa aataatcggg
     4501 aggaggagta ggtaaattag ggctacttat aactaatact ttcgtttgcg aacacctata
     4561 aatatgagca ttgatgttta ataataatta ttctagagat tgcttactgg tctaattatt
     4621 attatcacat catttaatgc tgtttgaaga tccaaaatat acgtatgact cgcttgttca
     4681 tgacaaacct cttagtcatc attagtacag atgaattgct ctgacgatat ttgactaact
     4741 gatatactct ttgatcttag ttagaattta ttgacttaaa aagtgggaaa cttgatataa
     4801 tatccatcgg aagctttcac tgaaaggatt ctttaaagaa gttacactat aatgttcatc
     4861 tctaaatact ttgtgacctt ataaagaagt agaaatacga gtgaaaaagt tagcgacagg
     4921 ctttggtctt agcaggatag gctgcctgat atgaaagcaa acgtaataca cggagatttt
     4981 aatccttgtg ggggtgccga aagactatca cttacaacta tgcaagcatt attagaaatg
     5041 ggaatagatt ttgaccttac aaccttgaag agtcctgata taccaaaatt agaaaattca
     5101 tatggcaaaa atcttgtctc agttatgaaa agcgtacaca aaattaatgt cataaatata
     5161 atggaagaac ttcggcaaca acaagaagaa aaagatcatg aagattacaa ttatgatatt
     5221 acaataaata caaatggtga tgcagctcct tattatcatt catctttttc aaaagataat
     5281 gctattactt attgccactt tcctagtaca aaatatcata tagaatctga aaatattgat
     5341 tatctaaaaa cagaccttgg catgacagaa aggtcaaatg ttttttcaaa ttataagaac
     5401 tctgaagaaa tcaataattc aaaaaattgc aaaaccaagc ctcagttcag cagaagaaaa
     5461 gaatattttg aaatattaaa atatggatat tggaatttga tgagaaattc tacagtgatt
     5521 accaattctg aattcagtcg cagggcaata gttaaagcat ttggattaga caacatatac
     5581 atactcagcc caccaataga tattgagact tttcgtaatg ttgcattaat ggcaaatggc
     5641 gatgatgatg aaacaaatga tatcattctg gtaatatcca gaatcgctcc tcacaagaaa
     5701 attgaaaatg caatcaagct tgcaaaaata ttaaaagata ataatgtagg caaaggaatg
     5761 aaaattgttg gaaatcttta ctattatttt tttgattatt attcagaact taagcagatg
     5821 gttctcgatc ttggtttgac tgactatatt acatttgaaa ttaatgctag cttagataag
     5881 cttctttcta tcatacgaga atccagagta tactttcatc caatgatagg agagcatttt
     5941 ggcatggcag tattagaagc tatggctggt ggactaatcc cagtggttcc aaatgaaggt
     6001 ggcgttatag agtttgttcc acaagaatat caatttaata caatagaaca cgctgctcaa
     6061 attataatat atgtttttaa tcatttgcca aagacagagc gaattaagct aagcgatgat
     6121 ataaataaat tctcaaattc ccattatatt gagggatttc agaccatatt aaatgaatca
     6181 ttatctcgga gaacgaaata atactatctg ctattgtaaa cattacattt ttaaatgaac
     6241 aagacatagt aatcaaaatg ccaaattgcg aaatgtgtgg gaacaattca gacaaatgtt
     6301 atactgttga cttgtcaatt acttctgact ttgataaaga tgaacaacca tttgaaagga
     6361 atagtacagt ttgttttgaa tgtaaaaata aaataataac cgtatttcca aaactagaag
     6421 atgaatattt ttaatattca aatattaaag ttataatagc tgcagtgatt gagtctaaag
     6481 aaatttggaa taaaagtata aagaaataat acaaaccatt agtattattt agtagttaaa
     6541 atgccagatt gtgaaatgtg tagtaataat tctgacaaat gtta
//
LOCUS       DASVEV010000084         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1191521, whole genome shotgun sequence.
ACCESSION   DASVEV010000084 DASVEV010000000
VERSION     DASVEV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191521"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..589
                     /locus_tag="VF222_04185"
     CDS             287..589
                     /locus_tag="VF222_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7141657.1"
                     /translation="MTSNPIRPSEIEKADEFIVIDVRESDELSEGNIENSIHLPLGSI
                     IRNARQGKLDKYKDKKIVTYCNSGYRGNIAAEELSKLGFNVFNLEGGFQGWMKSRK"
     gene            627..899
                     /locus_tag="VF222_04190"
     CDS             627..899
                     /locus_tag="VF222_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141658.1"
                     /translation="MVNKNTDAEDKIKTNKDSSIKLGLKPNTNQFLLLIVVNAFVGAM
                     IGLNKKLLILDKEMEKDAINNSNQYSQKYNKFYLHNIIIVLLLSII"
     gene            complement(1094..1222)
                     /locus_tag="VF222_04195"
     CDS             complement(1094..1222)
                     /locus_tag="VF222_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141659.1"
                     /translation="MNNLAIVVRIAVVSFLLVSSFFLQTQKAYEKDAMDKDMVLKS"
     gene            complement(1386..2321)
                     /locus_tag="VF222_04200"
     CDS             complement(1386..2321)
                     /locus_tag="VF222_04200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7141660.1"
                     /translation="MEKQSIAVVTGSSTGIGFETSLLLARNGFYTYATMRDLQKSNKI
                     EQIAHNENLPLKVLSLDVNNDMSVRNAIQKILEENEKIDILVNNAGYGLFGALEDMSL
                     DEIKRQFDTNLFGAIRTIKEVLPSMRKQKNGIIINITSIAGVVGIPAECIYVSSKFAL
                     EGLSESISYELQPFGIKVILVEPGVINTSFVPNIKFPNSNEDDNKHQTPSNKIEEESK
                     DNYYSNNDIKQKNSSHYSNTIDAFLSHYYPAMKNAPLPKEVATVILEAIKNASDSSDY
                     LFRYVIGEDSKTLADAKKNLSDSELHRFISNWLLR"
BASE COUNT          955 a          355 c          351 g         1026 t
ORIGIN      
        1 tatttagctt ttattattag aaacttgtct caacaaatga aaataaatat aataaaaatt
       61 attattgtag tatttatcaa gtcaaacaaa aattttatta ttttaccaaa atgacgaaaa
      121 ttggagactt tttcttttaa attaataaaa ttgagaaatg taatggaaaa agtacgtgaa
      181 atgcatcttt tgatatgtta taaaaatttt gttatgttat aaaaatacaa atcaggtgtt
      241 caatttgttg aataaattta aattgttaat atgaaaattc aatattatga ctagtaatcc
      301 aattcgtcct agtgaaatag aaaaagctga tgaatttatt gttatagatg tgcgtgaatc
      361 ggatgaactt tctgagggta atattgaaaa ttcaatccat ctcccactag gaagtataat
      421 acgaaatgca aggcaaggta aattagataa atataaagat aaaaaaatag ttacatattg
      481 taactcgggt tatagaggaa atattgcagc agaagaattg agcaaattgg gttttaatgt
      541 ctttaactta gaaggtggat ttcaaggatg gatgaaaagt cgaaagtaac tcatgaaata
      601 agatacaata tcctatagtt aaaaatatgg ttaataaaaa tactgatgct gaagataaaa
      661 tcaaaactaa taaagactct tcaattaaac taggtctaaa acccaatact aaccaatttc
      721 ttttgttaat agtggtgaat gcatttgttg gagcaatgat tggcctaaat aaaaaattgt
      781 tgattttaga caaggaaatg gagaaagatg ccataaataa ttccaatcaa tacagtcaga
      841 agtataataa attttatctt cataatatta ttattgtatt attattatct ataatttaac
      901 ctttaatcat ttatgagatt ttttatttat tattattatt cttgacttgt tgctactgct
      961 gtttgtttgt tcgttccatt ttttccacta tctataaaaa aggtgattag ttattagtca
     1021 tcatcatcat aatcttattc ttctccctct tccattgcat cttcttcctc ttctgtatct
     1081 tcctatgaat cttttagctc tttaacacca tatccttgtc catagcatct ttttcgtatg
     1141 ctttttgtgt ttgtaaaaag aaagaactta ctaataggaa tgaaactaca gctattctta
     1201 caacaattgc taagttgttc aatctttact aaataataga taatatgtta tttaacaata
     1261 cataaaaaga aattgaaatg tcaacttaag ctgctacctt gtatttagct tttattgtta
     1321 taaacttatt ttattttcaa atagttcaat tattttatta gagatatttt atgatagtca
     1381 tctcattacc ttagcaacca gtttgaaata aatctatgta attctgagtc agatagattc
     1441 ttttttgcat ctgctaaagt ttttgaatct tctcctatta catatctaaa taaataatca
     1501 gaagaatctg atgcattttt aattgcttca agtattacag tagcaacttc tttaggcaaa
     1561 ggagcattct tcatagctgg atagtaatga gagagaaatg catctatagt attggaataa
     1621 tgtgaagaat tcttttgctt aatatcatta ttagaataat aattatcttt ggattcctcc
     1681 tctattttat tcgatggagt ttgatgttta ttatcatctt catttgaatt tggaaattta
     1741 atatttggta caaaactagt attgataaca ccaggttcaa caagtattac ttttattcca
     1801 aaaggttgaa gttcatatga tatagattca cttaatccct ctaatgcaaa tttggaacta
     1861 acatatatgc attctgcagg aattcctact actcctgcaa tagaagttat atttattatt
     1921 attccattct tctgttttct catcgatggt aaaacttctt ttatagttct tattgcacca
     1981 aagagatttg tatcaaattg tctttttatt tcatctagag acatatcctc aagagctcca
     2041 aacaggccat aacctgcatt atttactaaa atatcgattt tctcattttc ctcaagaatc
     2101 ttttgtattg catttcttac agacatatca ttatttacat ctagagataa tacttttaat
     2161 ggaagatttt cattatgtgc tatttgttca attttgttac ttttttgcaa atctctcata
     2221 gttgcataag tataaaatcc atttcttgct agaagtaatg aagtttcaaa acctattcca
     2281 gttgaacttc ctgtaactac tgcaatactt tgtttttcca tattaaatag aaaattacaa
     2341 aaaatataaa atataattct ttagttagtt gacaatatta atagaaatga ttttgattat
     2401 cattgttttt gaatatatgt ttaacttgct attagaaggt aaatgtaatt gtccatgtag
     2461 tggggttcta atacatacta tttagctttt attagaagct tatcttattt taaacagttt
     2521 agttatctaa ataaacctta aatgaaaatt gtgtaatctg ataaaaaaaa gaaattttgg
     2581 tggcaatcct gtttgatttc tccattataa aatatcctca ataaaggaat gaaagctcta
     2641 acaaaatatc aaaatatcag caataaatcc caaatcaaaa tagtatt
//
LOCUS       DASVEV010000085         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1581194, whole genome shotgun sequence.
ACCESSION   DASVEV010000085 DASVEV010000000
VERSION     DASVEV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581194"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1161..1457)
                     /locus_tag="VF222_04205"
     CDS             complement(1161..1457)
                     /locus_tag="VF222_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141661.1"
                     /translation="MIFSNNECDNDFREEKHDQKNLMNKAAGYVIKKYIYEITKYEID
                     TIIKPIPTTADDLYEFIKNLALNDKEFHKYTVDNYKIRIYYNKEKDHFYVEYYN"
     gene            1707..1949
                     /locus_tag="VF222_04210"
     CDS             1707..1949
                     /locus_tag="VF222_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141662.1"
                     /translation="MKKSRIANPSLYVVITTIVYALLATTFFEMINVLGERENVVIDN
                     NNDTNNKTANVTQNVVETQCKSPCPPTAEMCVEMCA"
     gene            complement(2340..2786)
                     /locus_tag="VF222_04215"
     CDS             complement(2340..2786)
                     /locus_tag="VF222_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141663.1"
                     /translation="MLYIYEKIKFSLIMIGDLLYEGKGKLVGIRLLNADEYKIEHSVI
                     EEGKFKDIDITILGTFWTIPAGKNVTYVEGQGIITTKDNEDTATFRGYGIGTSKGQVS
                     VSTRGSVIYKSSSTNGKLSYLNNKIGVFEGEVDESGISTEKHWEWK"
     gene            3141..>3339
                     /locus_tag="VF222_04220"
     CDS             3141..>3339
                     /locus_tag="VF222_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141664.1"
                     /translation="MFIIELRITIIMFTTGAMPSSSSSSFNNNITKVIMTIFLVLSLS
                     IVTYTMIRNNDGDYESSFVVFA"
BASE COUNT         1156 a          455 c          452 g         1276 t
ORIGIN      
        1 cagttcccgc atctataaat agatgatata tcaaaacctt ttgtaagtat attatagata
       61 gtatcaacaa atttaacttc tacagggtca gtagcattaa aaatttttgg tttactcttc
      121 ataatatttt ttgtatttca gagttaaata acgtttggaa actaggttaa tttaattaaa
      181 agccatagtc catctccatc tacctagtag gagccaagga tacgtagata aaaaaagaag
      241 gccatgacca catattattc tttggactgt attaaaattc ataaaatata atattaatag
      301 ttggagataa taatttctta tttattaatt gtcttagcct tctaaggaat ttatatatca
      361 taacaataga aggctatgat tgggtatctg atttgatatg acttttggag tgtattaacc
      421 ttcgtcctac ataatattta atgattggag ataaattatt attatttatt caattgccat
      481 agcactctac ctggtgtctt tatttgttgt aggctatggc attcttgatg ttttcttgac
      541 agagtattat gtgtaaatat ttactcaata taatatataa taatttgaga caattttttt
      601 atttgttatt aataaattgt catagcctca tggtaccgga agtagtagta gatggaggct
      661 atgaatgaat acaacgacga ttagtattat taattgtgaa atattccatt taatagtttg
      721 atataaatta tttcgttaat ttattgccat aatcttacaa ttgtagtaga tgtggccgca
      781 atattacaat tagaagatta tgacaagagt acaacggcat tatccgagcc tgttgtacat
      841 aatattagta atcatataat ttaacaattt tagataattt aatgattgaa tttattgtca
      901 tagccagaac aagcaagcag tatagagcgt ttatagaaac tactaactac acatatgcat
      961 atctactacc tatcgttgtc cagactatga caaacatgat ttttccatat ttgttgagag
     1021 aagtatttaa gagatggtaa tttcattcat aacgcatatt gtatttatca actcttgtaa
     1081 acctatgttg aggctttaac aactaactac tatcatcaag caagcaagca atctacctat
     1141 ttattcattt atttatttaa ttaattataa tattcaacgt aaaaatgatc tttttcttta
     1201 ttataataaa tacgaatctt gtaattatca accgtatatt tgtgaaattc tttatcgttc
     1261 aatgctaaat ttttgataaa ttcatacaag tcgtctgcag ttgttggtat tggctttatt
     1321 atagtatcaa tttcatactt tgtaatttcg taaatatact tctttatcac atatccggct
     1381 gccttattca ttagattttt ctgatcatgt ttttcttctc taaaatcatt atcacattca
     1441 ttattactaa aaatcaaaag tcttattcat catcatatgt ttcatattat tattatgatg
     1501 ttctacctaa tggagaggaa ctttgttttg atatattgtt attgttctaa taatagtaat
     1561 atcgctagag taatatatta acaaataaaa tccgtaaaat ttctatgttc tttgtcggcg
     1621 aagaaaatat ttaccgaaat aattgtctaa atttttatta aaatatattt attgaaacat
     1681 attagatata ttaaaggcaa aagtatatga aaaaatcacg tattgcaaat ccatctttat
     1741 atgttgtgat aaccacaata gtgtatgcac tcctagcaac tacatttttc gaaatgatta
     1801 atgttcttgg agaaagagaa aacgttgtta ttgataataa taacgatact aataataaga
     1861 cggctaatgt aactcaaaat gtagtagaaa ctcaatgtaa atctccttgt ccaccaactg
     1921 cagaaatgtg cgttgaaatg tgtgcataga aataactaaa taaatatgta gatttttatt
     1981 ttttatttat tatatctttt ctcttttcta aagtttaatc taccaaattc attgggctca
     2041 gaggtttgac ggtcattttt gcctgtaatc ctaattaaaa aattaacaaa ttcatctagt
     2101 tcagaaggat ttgaaccttg ctaatgcatg ggttcagtat tctttttcaa actgaattag
     2161 tttaaataaa aataaacaaa ttaggatgtt caggtcgcaa tggtggatta gcaaaacata
     2221 ctacatttac attcagagtt attgatgtta ttgatgaaga ttcttcattt actttctgaa
     2281 taatattttg gtattgtgct acaaatatag aaaaaaattt ttatatttta tattatatgt
     2341 tatttccatt cccaatgttt ttcagtagaa atacctgatt catctacttc accttcaaag
     2401 actcctattt tgttattaag ataagaaagt ttcccgtttg ttgaagatga tttataaatt
     2461 acagacccac gagtactaac gctgacctgt cctttagaag ttcctattcc atatccacga
     2521 aatgttgcag tatcttcatt atctttggta gttattatcc cttgcccttc aacatatgtt
     2581 acatttttac ctgcagggat tgtccaaaat gttcctagta ttgtaatgtc gatatctttg
     2641 aactttcctt cctctatcac agaatgttca atcttgtatt catctgcatt taagagtctt
     2701 atacctacaa gtttgccttt tccttcatat aataagtctc ctatcataat cagggagaat
     2761 tttatttttt catatatata aagcataatc tattaaaacg acttaaaata tcagatggaa
     2821 atttagacta caacagagta gttatttgta gaatgtgatg taagaatttt aacacatttc
     2881 taaaactatg gggaaaataa tcgtccacgt agtagggttc tattacatag tattttgctt
     2941 ttattattag aaacttattt tgtttttcat gttcttgttt taattaaata atgaatgatg
     3001 aataattaga tgttttatta aaaaaaagaa aaaggaagga tgataatata acttagttaa
     3061 attctaatat aaagaactgt aagaattata atagaaatat acaatcttca gtatagctta
     3121 taataaacta gttattttaa atgtttataa tagaattaag aataacgata ataatgttta
     3181 caacaggagc aatgccatca tcatcatcat catcattcaa taataatata actaaagtta
     3241 taatgacaat atttcttgtg ctatcattat ctatagttac atataccatg attagaaaca
     3301 atgatggtga ttatgaatca tcctttgttg tatttgcgc
//
LOCUS       DASVEV010000086         5823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1192728, whole genome shotgun sequence.
ACCESSION   DASVEV010000086 DASVEV010000000
VERSION     DASVEV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192728"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VF222_04225"
     CDS             complement(<1..1497)
                     /locus_tag="VF222_04225"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX7141665.1"
                     /translation="MVYFLFFIILLILQLPLSCIYSQKDEPVISEKIGKQFLPVGSYP
                     VGIGINSVTNKLYVANQFSNTVTVIDGNTNNIESTIKVDNFPYDLEVNPYNNRIYVTN
                     RGSNTVSIIDGSTNQRLSNINVGESPVGISINPSENRIYVTNLGKNSSLSIIDGISNK
                     VTDTVELNGLFYGVVVNPLTKTIYLSNIKNHTISVLDEKTKNVVNNISVGKIPTGINI
                     NYKSDLIYVTNYLSNSISVIDGKTNKVNSTIKVGQQPVDTIINPLTNKIYVSNIGDNT
                     VSVIDGTTNKKIKDISVKPTNDKNNGNNDLILDIPINLTFPLLANKLSLNPNTNLIYI
                     TNTVANGVLVIDGKTDRVVVQLFVEINPENAGTIECNNLKLPSQSFWLHPINTEIKCT
                     AHASRGYSFDFWSNMISDNKNPITLNVTDNNLLVSNFKPAISSETYIGMVAAIIGSTS
                     IFAGWYYKRRERSYLNTSMNRIDFTYDALKKDKLEGVKQLEEIRKEITN"
     gene            1674..2198
                     /locus_tag="VF222_04230"
     CDS             1674..2198
                     /locus_tag="VF222_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141666.1"
                     /translation="MDNFCKLNYLSSLSFILIGVFLYIISDGQQSFSAQDGEEETSDI
                     NNNEESTTNDIFSDLSRNLPSIDEKTLKENGEPLESDEPLFGNPDTETLNDNNGMDNS
                     ENIVESNDQRTPSQSIEKSFSSGFSDSGKETDIINEDGGDSEDNIETSSSSSNEVSND
                     ENNNNNNNIINNPD"
     gene            2317..3273
                     /locus_tag="VF222_04235"
     CDS             2317..3273
                     /locus_tag="VF222_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141667.1"
                     /translation="MCYKNIVPSLAIILFSLIIILVPIKSFGEFPISIVDQKIFKDLN
                     GDWNLVGVVQNNAGTPIEVKVGVNITQSEKQSSILTSNTFTNVIYPYVAAPFKFTISG
                     NEFFNGFAYPIETKEVAKPLLMDLTQNYSNLATGDEKALVGTIKNTGPNIIKNIAVYA
                     SVHSINGTQIDSVKSSMIPVIYPGEDVRYKILPDPAIKDGVYYYSCAGLDVNAPISTI
                     DIGEGQYLVFNLESASMISDFRYENSSDSIIFEVKPYNPSGGPTNFKIPQIFSNQKIE
                     ILLDGKQYENKLVTMNGKTITIDIFIPKDKHVLKISGIRTQL"
     gene            4067..4426
                     /locus_tag="VF222_04240"
     CDS             4067..4426
                     /locus_tag="VF222_04240"
                     /inference="COORDINATES: protein motif:HMM:NF042555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX7141668.1"
                     /translation="MDYDNLCKAVIDIDAEIRFAGICDESGEIKHGGQREGVVDMLSN
                     EETRKSNLQALARWGLRNSLSSKIGRGKYAMAEYEKIKRITIPLDDDHLLLITTEVQA
                     DHGKIISNTLKLLSVNY"
     gene            4426..4662
                     /locus_tag="VF222_04245"
     CDS             4426..4662
                     /locus_tag="VF222_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7141669.1"
                     /translation="MAEAFVLITCEELHEEKIINELTKIKEIVTINRVRGPYNILVKI
                     KGETIDTVIETITSEIKTNDKIKYILTLNVKQNI"
     gene            complement(4795..5181)
                     /locus_tag="VF222_04250"
     CDS             complement(4795..5181)
                     /locus_tag="VF222_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141670.1"
                     /translation="MAVADNFGIEDRKAEEAITWMNSYALENNKKFEAKLEGYLLTTK
                     NFGNFEIISWKGDWSFARNLIIKVSRKLSTKVIESGYHQKNSFFLSLIGANKEVAKVY
                     KKGGIIGNLILAKKSGKWIVKSEKLI"
     gene            complement(5320..>5823)
                     /locus_tag="VF222_04255"
     CDS             complement(5320..>5823)
                     /locus_tag="VF222_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141671.1"
                     /translation="NLINSLSEKDQDYYFIGIEKQESYFDKAKYNIKSQNVKILHTLF
                     EDVLPLFEDSSIHKIISVLPDPKYIDKNYSRNWEILYKTIYKKLVKQGKFILITEITD
                     ALFQPVSNYEFYKEVENLKQIFQSIGFIILHAFEGYQENYMTSFLQEFNGDPERIRII
                     NFEFTKN"
BASE COUNT         2189 a          745 c          830 g         2059 t
ORIGIN      
        1 atttgttatt tcttttcgta tctcttccaa ttgtttaact ccttctaact tgtctttttt
       61 taaggcgtca taagtgaagt ctattctatt catgcttgta tttagataac ttctttctct
      121 tcttttataa taccaacctg caaatattga agtagaacca attatagcag ctaccattcc
      181 aatatatgtc tcacttgata tagccggttt gaaattagat actagtaaat tattatctgt
      241 tacgtttagt gttatagggt ttttattatc tgagatcata tttgaccaaa aatcaaatga
      301 gtatcctcta gatgcatgtg cagtacattt tatttcagta tttataggat gcaaccaaaa
      361 actttgcgac ggtaacttta aattgttgca ttctatagtt cctgcatttt ctgggtttat
      421 ttctacaaat aactggacaa ctaccctatc agttttacca tcaattacca aaacaccatt
      481 tgcaactgta tttgttatat aaattagatt tgtgttagga ttaagagaca atttattagc
      541 taaaagtgga aaagttagat ttataggtat atcaagaata agatcattat taccattatt
      601 tttatcgtta gttggtttaa cggatatatc tttaattttt ttattagtcg ttccatctat
      661 tacagatacg gtattgtcac caatattaga tacatatatt ttattcgtta aagggttaat
      721 tatggtatca acaggttgct gtccaacttt tatagttgag tttactttat tggtttttcc
      781 atctattaca gaaatagagt tagaaagata attagttaca taaattaaat cggatttata
      841 atttatattt attcctgtag gtatctttcc aaccgaaata ttattaacga cattttttgt
      901 tttttcatct aaaacggata tagtatgatt tttaatatta gaaagataaa ttgtttttgt
      961 taatggatta accactacac cataaaaaag gccattcaat tccacggtat ctgtaacttt
     1021 attactaata ccatctatga ttgataaaga agaattttta cctagatttg ttacatatat
     1081 tctattttct gaaggattta tcgatattcc tacaggggat tctccaacat tgatattgga
     1141 cagtctttga tttgtagaac catcaataat agaaacagta tttgaacctc tattagtcac
     1201 atatattcta ttattataag gatttacttc caagtcatat ggaaagttat ctactttgat
     1261 tgtgctttca atattatttg tatttccatc tattactgta acagtattag agaattgatt
     1321 tgcgacatat agtttgttag taacagagtt tattcctatt ccaacaggat agctacctac
     1381 aggtaaaaat tgcttaccta ttttttcaga aataactggt tcatcttttt gtgaataaat
     1441 acatgatagt ggtaactgta atattaataa tattataaag aaaagaaaat aaaccaatga
     1501 taatctttgt attaaaccat tcaacaaata ttattctatt ttgataatat aaaaataata
     1561 ttttatcaat aaagcaacaa ttatatactc tctatataaa aaggtagaaa aaatcctatt
     1621 ataaaataat catatgatat atttataaaa agaagaggta ataatataac agaatggata
     1681 atttttgcaa actgaactat ttatcatctt tatcattcat actaataggt gtatttcttt
     1741 atatcatttc tgatggacaa caatcatttt cagctcaaga cggagaagaa gaaacttctg
     1801 atataaataa caatgaagaa tcaactacta atgatatttt tagtgattta agcagaaatc
     1861 ttccaagtat tgacgaaaag acattgaagg agaatggaga gcctctagag agtgacgaac
     1921 cattattcgg aaatccagat acagaaacac tcaatgacaa taatgggatg gataatagtg
     1981 aaaatatagt agaatctaat gatcagagga cgccaagtca aagtatagaa aagtctttta
     2041 gtagtggatt tagtgatagt ggtaaagaaa cagatattat taatgaagat ggaggcgaca
     2101 gtgaagataa tatagaaaca tcttcttctt ccagtaatga agtaagtaac gatgagaata
     2161 ataataataa taataatatc ataaacaatc cagattgatt aaaaaggtaa ataggataag
     2221 agttagaaag aaaaaataat ttaataaata attcatgaaa agaaatttaa ttctgtctat
     2281 ctcatactat tttaaattat gaagtttagt aatagaatgt gttataaaaa tatagttcca
     2341 tctttggcta ttatattgtt ttctttaata attattctag tgcctatcaa atcctttgga
     2401 gaatttccaa tttcaattgt ggatcaaaaa attttcaaag atttaaatgg agattggaat
     2461 ttagttggag tagtacaaaa taatgcaggt acgcctatag aagttaaagt aggtgtaaat
     2521 ataacccaat cagagaaaca atcttcaatt ttaacaagta atacatttac taatgtaata
     2581 tatccatatg ttgctgctcc atttaagttt accattagtg gtaatgaatt ctttaatggg
     2641 tttgcctatc cgattgagac caaagaagta gcaaagccat tactaatgga cttaacacag
     2701 aattattcaa atcttgctac aggcgatgaa aaggctctag ttggtacaat caaaaataca
     2761 ggtcctaata taattaaaaa tattgcagta tatgcttctg ttcattcaat taatggtact
     2821 caaatagatt ctgtaaaaag ttccatgatt cctgtcattt atccagggga agatgttaga
     2881 tacaaaattc ttcctgatcc tgcaataaaa gatggtgtat attattatag ttgtgcagga
     2941 ttggatgtta atgctcctat atctacaatt gatattggag aaggacaata tcttgttttt
     3001 aatttagaaa gtgcctctat gattagcgat tttagatatg aaaattcgtc agatagtatt
     3061 atttttgagg tcaagcccta taacccctct ggtggaccta ctaattttaa aattcctcaa
     3121 atattttcaa accaaaagat agaaatttta ctggatggaa aacaatatga aaataaatta
     3181 gttactatga atggtaaaac aattactata gatatattca ttccaaaaga taaacatgta
     3241 ctaaaaatat caggaattag aactcaacta taatatttac atgatacaat ttaattaata
     3301 gtcgaggaga gaggctaatt gaaactataa ttttagtttt tcaagaaaaa ttattttatt
     3361 gaataaattc actcatgaat aaatgttata taatattatt atataatttc tattaataac
     3421 tctcttatat tggattctcg ttatagaatt agacactgtc tacactgaat tcgacggaaa
     3481 taacactgtt gaatgatgtg atgtggtatc agctgacttg tgcaggaagg ctataccaaa
     3541 gctgaaaatc gatgggtaac gatgagctaa ataattttat ttagctcatc gtattttctt
     3601 aatatttgat tttgaatata ttaataataa aaaaactatc atttcaaaac ttttagtaat
     3661 tttgataata gatgtaattt ttatttgaaa ataaacaaaa agcaaaatta attaaaaaat
     3721 attcataaaa gatataattt aggagatata tatataaata gactaaactt atgaaatctt
     3781 ccagaatatc aaacaagaaa gaaagaagat tttttaacca tgaatatctg agagatagtc
     3841 ttctacctag atataaagaa tgtgagtctt gtaaaattag aacgcaactt acatgtttaa
     3901 tatgtagctt ttgttggagt tgccattgga agatagaaga attagctaaa attccagatt
     3961 atatataaaa attaatacaa aaatagagat ttaattactt taaaattttt caaaataaat
     4021 tgtaaattta taaactatta atatttgtat ttttgaatat agtaatatgg attatgataa
     4081 tctctgtaaa gctgtaattg atattgacgc tgaaataaga tttgcaggaa tatgtgatga
     4141 atccggagag ataaaacatg gaggtcaaag agaaggagta gtagacatgc taagtaatga
     4201 agaaacaagg aaatcaaatt tacaggcttt agcgagatgg ggattgagaa attctttatc
     4261 ttccaaaata ggaagaggaa aatatgccat ggcagaatat gaaaaaatta aaagaattac
     4321 gattccatta gacgatgatc acctattgct tattactaca gaagtacaag cagatcatgg
     4381 taaaattatt agtaatacgc tgaaattact aagtgtaaat tattaatggc agaagccttt
     4441 gttttaataa cgtgtgaaga acttcatgaa gaaaaaataa taaatgaact aactaaaata
     4501 aaagaaatag taacaataaa tagggttagg ggtccatata atatacttgt caagataaaa
     4561 ggagagacaa ttgacacagt aatagagaca attacatcag aaatcaaaac aaatgacaaa
     4621 attaaatata ttttaacatt gaatgtcaag cagaatatat agaacaggaa gaaaaagatg
     4681 aagaaacttc taaatccatt taaaaaagac tttttttatg caaatcaatt gcaattagca
     4741 atagtcaaaa taaaataaat cctatatata gtgtcttaaa agaggatctt aatatcatat
     4801 taatttttca gatttaacaa tccatttacc agattttttg gctaaaataa ggttaccaat
     4861 tatacctcct tttttataga cttttgccac ttctttatta gcaccaatta aagagagaaa
     4921 aaatgaattt ttctgatggt aacctgattc aattactttt gttgataatt tcctgctgac
     4981 cttaattatc aagtttctag caaaggacca atctcctttc catgaaataa tttcaaaatt
     5041 accgaaattc ttggttgtta ataaataacc ttctaatttt gcttcaaatt ttttattatt
     5101 ttctaaggca taggaattca tccacgttat tgcttcttct gcctttcggt cctctatacc
     5161 aaagttatct gcaactgcca agttatagaa ttaacacagt gtggctatta aagagtatat
     5221 tatttaaatc acaaaaacaa gtttcactat ctaaataact tggaaaaaat ttacagttgt
     5281 cgttgcctga gacaaccaga tcgactaatt agatttaggt taatttttag taaattcaaa
     5341 attaattatt ctaattcttt caggatctcc attaaactct tgaagaaaag acgtcatata
     5401 attttcttgg tacccttcaa aagcatgtaa tataataaat cctattgact gaaaaatttg
     5461 ttttaaattc tcgacttctt tataaaattc ataatttgat actggttgga ataacgcatc
     5521 agttatttct gttattaaaa tgaatttccc ttgtttcact aattttttat aaatagtttt
     5581 gtataagatt tcccagtttc tgctataatt tttatctata tatttaggat caggtaaaac
     5641 tgatataatt ttatgaatag aactatcttc aaataaaggt aaaacgtcct caaataaagt
     5701 atgaagtatc ttaacatttt gcgatttaat attatattta gctttatcaa aataactctc
     5761 ttgtttttct atgccaataa aataataatc ctgatccttt tctgagagtg aatttattaa
     5821 att
//
LOCUS       DASVEV010000087         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_804882, whole genome shotgun sequence.
ACCESSION   DASVEV010000087 DASVEV010000000
VERSION     DASVEV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804882"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..260
                     /locus_tag="VF222_04260"
     CDS             36..260
                     /locus_tag="VF222_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HEX7141672.1"
                     /translation="MGNNPEFGLAVMNRVIKKAGAERVSDEASDTLRTIIEEIANNIS
                     SNAIDLANHAGRKTIKSEDILLAYKNTYKK"
     gene            388..470
                     /locus_tag="VF222_04265"
     tRNA            join(388..423,435..470)
                     /locus_tag="VF222_04265"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:420..422,aa:Cys,seq:gca)
     gene            complement(509..853)
                     /locus_tag="VF222_04270"
     CDS             complement(509..853)
                     /locus_tag="VF222_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141673.1"
                     /translation="MSSNNNGKESRFKGKHAHSTEYRRLIWEMIVWPLIIEKNRPYFS
                     IDEYQSKRVLFSKESNIPSSKVSGGLISLVVKGILIKEEHIYSLHYRLIPYLRKKAIL
                     TYGQALKETYTK"
     gene            1022..1183
                     /locus_tag="VF222_04275"
     CDS             1022..1183
                     /locus_tag="VF222_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141674.1"
                     /translation="MSLPQILLAILPSGTVINETIFFSYIFILMGLLVTAIWLRRKRT
                     KKTSTESPE"
     gene            complement(1214..1927)
                     /locus_tag="VF222_04280"
     CDS             complement(1214..1927)
                     /locus_tag="VF222_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7141675.1"
                     /translation="MNRNELLKIKDETYKCFDEKSSTDLNLFESIIGYDDIKKIFKMS
                     LLSDKAVHILLVGPPASAKTLFMLEFLKLERSYFTLGSHSTKAGMIDYLFDFRPRYLL
                     VDEIEHMPYKDQTSLLSIMETGIITETKSQKTRSTQLKTWIFATCNNKEKLLSPLLSR
                     FIIFHLKPYNLSKFIEVSNGILIKNGISFDVANEISHVVWTKLKSRDIRDCIKISRLA
                     KTVEDVEWIAKTLNQYRKK"
     gene            2053..2235
                     /locus_tag="VF222_04285"
     CDS             2053..2235
                     /locus_tag="VF222_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141676.1"
                     /translation="MTDVSINEDEKHLVLFALNQLAEMIEDGNLKEEDFKDTEYSFIN
                     LSSIYELKDRLEAEIM"
     gene            complement(2295..2948)
                     /locus_tag="VF222_04290"
     CDS             complement(2295..2948)
                     /locus_tag="VF222_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141677.1"
                     /translation="MAIDNFIQKSSGISSSLPPLMATKIKEILKKDNKDDTIGIYSKE
                     NVKIFDILVNSKREICIACVSLDQIFSFNYIEVFKSILKNSIKLKILLCDPHSDLLNH
                     IKYLTVFPNIYESLTSCLSKLCNMKIVSLSTKELSNVEIRIYKTILPHNLIIIDRDIH
                     GSGIILVEPYLFGIKSQNRKIYQIYKRKNQEIFDLHFKSFLKLWETSPEYSCEWEEL"
     gene            3218..3355
                     /locus_tag="VF222_04295"
     CDS             3218..3355
                     /locus_tag="VF222_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141678.1"
                     /translation="MFEVNSEKYTRYEEARANHWRCDKCGTRFSTFKKLRKHKKDIHA
                     Y"
     gene            3503..3640
                     /locus_tag="VF222_04300"
     CDS             3503..3640
                     /locus_tag="VF222_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX7141679.1"
                     /translation="MEGYCVKCKSKREMKDEKKVTMKNGRPATQGICSVCGTKMFKIG
                     K"
     gene            3902..>4790
                     /locus_tag="VF222_04305"
     CDS             3902..>4790
                     /locus_tag="VF222_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141680.1"
                     /translation="MQSIDSQAQTTPKTFENISVKITSPAEGKKIPSGELTISGISSD
                     TQNKNCIVFVDWNDLKPFQKVRAAGPGGIDDFSEWIYTYDVTYHKIIAGNNELTSKIT
                     CLDGSKPLTKWNSINVTGYLKTSSLLPIIQNNTTSLESSSKQVPLKTILPGANQNNNT
                     TSLEHNTTSLQLTNASPDEHNTTSLQLTNASESAVEILIPNESSDDVIDNTTKSISAS
                     AQSNSTNATNSNSKQFTLGNARIDLDEQSEAPIDEELDNQTMVTPDNQTMVTPDNQTM
                     VTPDNQTMVTPDNQTMVTPD"
BASE COUNT         1725 a          675 c          708 g         1682 t
ORIGIN      
        1 attagatttt taactatgct gagcattttt atattatggg taataaccct gaatttggat
       61 tagctgtaat gaatcgagta ataaaaaagg ctggtgcaga aagagtaagt gatgaagcat
      121 cagatacttt aagaacaata atagaggaaa ttgcaaacaa tatatctagt aatgctatag
      181 atcttgcaaa tcatgccggc agaaaaacaa ttaaatctga agatatttta cttgcctata
      241 agaataccta taaaaaatga atagatttta atataaatta ttttaatgag tttaaacagt
      301 caaatattac tataataaat aataaattaa ttttgttaat ggaaaagaac tcaaatttat
      361 atgtctcatt tcaattacta attacgaggt ggggtggcag agcggttatg cgttcgcctg
      421 cagaaattgt ggaaagcgaa tatatagggg ttcgactccc ttccccacct tctcacttaa
      481 tttaaagcaa atttcctgaa ttaggagatt acttggtata agtttccttc aatgcttgtc
      541 cataagttaa gattgctttt tttctgagat aaggaattaa cctataatgt aatgagtaaa
      601 tatgttcttc cttaattaaa attcctttta ctactaatga tattaatcca ccagatactt
      661 tcgaagatgg tatattactt tcttttgaaa ataaaactct tttagattga tattcatcta
      721 ttgaaaagta tggtctattt ttttctatta tcaaaggcca gacaatcatt tcccaaatca
      781 accgtctata ttcagttgaa tgagcatgtt tccctttgaa gcgggactct ttaccattat
      841 tgttggagga cattgttcat attttttatg tatttaatgt tggtttaggt tttataatct
      901 aatactttta aatgaatatc tctcccaatc ctataatact acaacagaga ataataagta
      961 tctacaaaaa aaattttaag atttggaaag caatataaca gttatatggt ccggaataga
     1021 tatgagcctg cctcagatct tattagcaat acttccctct ggcactgtaa taaatgaaac
     1081 aatatttttt tcttatattt ttatcctaat gggtttatta gtaactgcaa tatggcttag
     1141 gaggaaacga actaaaaaaa catctaccga atcaccagaa tagcatttga atgccagcca
     1201 tttactattt tttctatttt ttccgatatt gatttaaagt ttttgcgatc cattcgacgt
     1261 cttcaactgt ttttgctaac ctggaaattt ttatacaatc tctaatatct ctagatttta
     1321 atttggtcca aacaacgtga gaaatttcat tagcaacatc aaaggaaata ccatttttga
     1381 taagaattcc gtttgatact tctataaatt tggacagatt atatggtttt agatggaaaa
     1441 taataaatct agaaagaagt ggagataata atttttcttt gttattacat gttgcaaaga
     1501 tccatgtttt tagttgtgta ctacgagtct tttgtgactt tgtttcagtt attatccctg
     1561 tctccatgat acttagtaat gatgtttggt ccttgtatgg catatgttct atttcatcca
     1621 ctaataaata tcgtggacga aaatcaaata aatagtctat catccctgcc ttggtactat
     1681 gactccctaa agtaaaatac gacctttcta gcttcaggaa ttctaacata aataatgttt
     1741 tggctgaggc aggagggccc actagtaaga tatgtactgc tttatcggat agcaacgaca
     1801 ttttaaaaat tttttttata tcatcgtatc ctatgatact ttcaaaaaga tttagatctg
     1861 tactactttt ttcgtcaaag catttataag tttcatcttt aatctttaaa agttcatttc
     1921 tgttcattta taataagtaa agattatctc tttataggtt ttattgattt ttaatattaa
     1981 tgccagtaag ttcttgaatt ttagatatta atctaggaag agaaaaatat aggcgacaag
     2041 agagaatatt cattgacgga tgtttcaatc aatgaagatg aaaaacattt agttctattt
     2101 gcattaaatc aattagctga aatgattgaa gatggtaacc ttaaagagga agacttcaag
     2161 gacacggagt atagtttcat taatttatct tctatatatg agctcaaaga tagattagaa
     2221 gcagaaatta tgtagttatt gtatttttct attggtgttt tttatgatca aggggtaaag
     2281 agtagagtaa atttttacaa ttcttcccat tcacaagaat attcaggact agtctcccac
     2341 aattttaaaa aagatttgaa atgtaaatca aaaatttcct gattttttct tttatatatc
     2401 tgataaatct ttcgattttg tgattttatt ccaaagagat aaggctccac gaggataatt
     2461 ccactaccat gaatatctct gtctattatt atgagattat gaggtagtat tgttttataa
     2521 attcttattt caacattcga taattcttta gtagataatg atacaatctt catgttacat
     2581 agcttggaaa gacatgatgt aagactttca taaatatttg gaaatacagt caaatattta
     2641 atatgattta gcaagtcaga atgaggatca caaagtaaaa tttttaattt tatactattt
     2701 tttaatatac ttttaaatac ttcgatataa ttaaaagaaa aaatctggtc aaggctgaca
     2761 caagcaatac aaatctctct tttagaatta actagtatgt caaagatttt tacattctct
     2821 tttgaatata tacctatggt gtcatcttta ttgtcctttt ttaaaatctc ctttatttta
     2881 gtggccataa gcggaggtag agatgacgat atgccactac tcttttggat aaaattatcg
     2941 attgccaatg aagaaaaaat actttgaaga aataaaaatt tttatttttt aaagttgata
     3001 ttattaatct ttttataaaa aaagggtata aataatcata aaggattgct tttataggtg
     3061 ttaagtatgg tgtattcttt ttttaagtaa aagtcagaga aaattagcta acagtctttt
     3121 taatgacaat taatatataa caaagttgtc tgctgttcct tctaaattaa aatttcgact
     3181 taattataag cctattaatt atgatgttaa gaccggtatg tttgaagtaa attctgaaaa
     3241 atatacaaga tatgaagaag caagagcaaa tcattggaga tgcgataaat gcggtactcg
     3301 cttttcaaca tttaaaaaat taagaaaaca taaaaaagat atacatgcct actgaaacag
     3361 agaaaattgg ggatttaata ttatgaagta tatattttac taatttctta atttgagtat
     3421 aattttccaa tttagcacat aactctttta tttcataaga aatacatcaa tacttttatt
     3481 gtggtctatt tactgaatta atatggaagg atattgtgtt aaatgcaaat caaaacgaga
     3541 aatgaaggat gaaaaaaagg ttactatgaa aaatggaaga cctgctactc agggaatatg
     3601 tagtgtttgt ggaacaaaaa tgtttaagat aggaaaataa tcacataata taataaaaca
     3661 ttataattac atgtaatgtt tttcaatttt tttaaagaaa atttataaat attcttcctt
     3721 ggctaactta aaatataata aaattctgat aattgtttga aataatatag tagaaagttc
     3781 tacgagatat attattaatt taaatatagt gtaattttga ttctatatta tgaaatttat
     3841 gtttgtgact ttttttataa ctattctaat tggattcttg ggcataattt ttttctatga
     3901 tttgcaatct attgactctc aagctcaaac caccccaaag accttcgaaa atattagtgt
     3961 aaaaatcaca tcgcctgcgg agggaaagaa aattcctagt ggagaattga ctatttctgg
     4021 tatttcaagt gatactcaaa ataaaaattg tattgtgttt gtagattgga acgacttaaa
     4081 accttttcaa aaagtaaggg ctgctggccc aggagggata gatgattttt cggaatggat
     4141 ttacacttac gatgttactt atcataaaat aattgctgga aacaatgaat taacatcaaa
     4201 aataacatgt ttagatggaa gtaagccttt aacaaaatgg aattctataa atgtaactgg
     4261 ttatttaaaa acttcctcgt tattacctat tatacaaaat aatacaacct cattagaatc
     4321 atcctccaaa caagtcccat tgaaaactat tctaccgggg gcaaatcaaa acaataacac
     4381 tacttctctt gaacacaata caacctcatt acaattgact aatgcatctc ctgatgaaca
     4441 caatacaacc tcattacaat tgactaatgc atctgaatca gctgtcgaga tcctcattcc
     4501 aaatgagtca tcagatgatg ttattgataa tactactaag tctatttctg caagtgcaca
     4561 atctaattcg actaatgcaa cgaattccaa ctcaaaacaa tttacattag gaaatgctcg
     4621 aatagacctg gatgaacaat cagaagcacc aatcgatgaa gaattagata accaaacaat
     4681 ggtaacacct gataaccaaa caatggtaac acctgataac caaacaatgg taacacctga
     4741 taaccaaaca atggtaacac ctgataacca aacaatggta acacctgata
//
LOCUS       DASVEV010000088         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_28058, whole genome shotgun sequence.
ACCESSION   DASVEV010000088 DASVEV010000000
VERSION     DASVEV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28058"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1748
                     /locus_tag="VF222_04310"
     CDS             180..1748
                     /locus_tag="VF222_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141681.1"
                     /translation="MKASVIDLGYNSIKLVNYNIIQKNGNYRSYYDKSIRVKLGEDMH
                     MTNLLRSDAIDRTIESIKYFSDIIHIESIEHVIPFATSAVREAENKKIFLQKVEEETG
                     FKFKVLSSEEEAFYSYLGALNSLCIPNCLFFDLGGGSLELVYTENYKIKKIISLPLGS
                     LKLTQKFFSNTTEKNNNDRGSFEKSYINLQNYLVESLPSKKDLGIGNDSITLVGVGGT
                     LRALARYDQLIKEYPLKKLQNYDLEYSSLSIIRNHLYSMSTKEISTIDVIGSNRAETI
                     NTGLCIVYLLMSKLNFQNLIVSTNGIREGIVFDLVRSLKNSTNSVLNFKTRNIKFDKS
                     PLENCEKTSKSSTELPIFINQLLSLEILNNYEYDILKKAILYLSKISNTVSYLGKFYF
                     LLDEDIPNINHKQQVILALSLISICKPKIVSELSENYVSLLRPSSKKKNGKKIVEKIS
                     ICLQLCKILKKIKWIKIIEFNKENRKMLISITLHKEVKIHELLLKEILKRFEYIFNLS
                     LEYSFFQYPKEMRR"
     gene            1753..>2124
                     /locus_tag="VF222_04315"
     CDS             1753..>2124
                     /locus_tag="VF222_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141682.1"
                     /translation="MIVQKTQQKVKENRYGVTGKLVVVEGIDGSGKSTQIRLLEKWLR
                     FKNIPVFLTEWNSSEAIKDVTAKGKKKEKLTPTTFSLLHATDFADRYERNIFPLLRAG
                     YVVLADRYIYTAYARDIVRGCS"
BASE COUNT          872 a          262 c          314 g          676 t
ORIGIN      
        1 tgataccgat agaaattata acacgaatca caataatatt atactcaaaa aagcaggttt
       61 atcaaggatt aaaataactt ctactgtccc cagattgaaa ggagaattaa gatggttact
      121 tacaccaaga attttaaaga agctatccta acctttatta attatatcag gagaaaaacg
      181 tgaaggcatc ggtaatagat ttaggatata attctataaa attagttaat tataatatta
      241 ttcaaaaaaa tggtaattat agatcatatt atgataaatc gatacgagta aaactcggag
      301 aagatatgca catgacaaat ctacttagat cggatgcaat agatcgtact atagaaagta
      361 taaaatattt tagtgatata atccatattg aatcaattga acatgttata ccattcgcaa
      421 ccagtgcagt gagagaggct gaaaataaaa aaatatttct ccaaaaagta gaagaagaaa
      481 ctggttttaa atttaaagta ttatcttcag aagaagaggc attttattcg tatttaggag
      541 ctctaaactc tctttgtata cctaattgtt tattctttga tctcggtgga ggtagtctag
      601 aacttgtgta tactgaaaat tataaaataa aaaaaataat ttcacttcct ttgggttcac
      661 taaaactaac tcagaaattt ttttctaata ctacagaaaa aaataataat gatagaggct
      721 cctttgaaaa aagttatata aacctacaaa attatttagt agaatcttta ccaagcaaaa
      781 aggatttagg aataggtaat gattctatta cattagtagg agtaggagga acattaagag
      841 cattagccag atacgatcag ttaattaaag aatacccatt aaaaaaactt caaaattatg
      901 atttagaata tagttctcta tctataataa gaaatcactt gtacagtatg agtacaaagg
      961 agatttctac tattgatgta ataggtagta atagagcaga aacaatcaac acaggtttat
     1021 gtatagttta tttgctcatg tcaaaattaa attttcaaaa tttgattgta agtactaatg
     1081 gtataaggga aggaattgtg ttcgatcttg ttcgatcatt aaaaaattct accaatagtg
     1141 tactaaattt taaaactcga aatattaaat ttgataaatc acctttagaa aattgtgaaa
     1201 agacttcaaa atcatctaca gaactaccta tttttattaa tcaactgttg tctcttgaaa
     1261 tattaaataa ctatgagtat gatatattaa aaaaagctat tctttattta tcaaaaattt
     1321 ctaatactgt tagttattta ggtaaatttt attttctact tgatgaagac attccaaata
     1381 tcaaccataa acagcaagtt attctagcgt tatcactaat atcaatttgt aaaccaaaaa
     1441 ttgtttcaga actttcagaa aattatgttt ctttattaag accgtcctca aaaaagaaga
     1501 atggaaaaaa aattgttgag aaaatttcta tttgcttaca attatgtaaa attctgaaaa
     1561 aaattaaatg gataaaaata atagaattta ataaagagaa taggaaaatg ctaatatcta
     1621 taacgttaca taaagaggtt aaaattcatg aactcttact aaaagaaata ctaaaacgtt
     1681 ttgaatatat attcaatctt tcactagaat actcattttt tcaatatcca aaagaaatga
     1741 gaagataaaa aaatgattgt acaaaagacc caacaaaaag taaaagaaaa tagatatgga
     1801 gtaacaggaa aacttgttgt agtagaagga atagatggat caggtaaatc tactcaaata
     1861 agacttttag agaaatggct taggtttaaa aacatacctg tatttttaac agaatggaat
     1921 tcttctgagg ctataaagga cgtaactgct aagggaaaga aaaaagaaaa attaactcca
     1981 actacatttt ctttacttca tgcaactgat tttgcggatc gctatgaaag aaatattttt
     2041 cctttgttaa gagccggtta cgtagtacta gctgatagat atatctatac agcctacgct
     2101 agagatatag taagaggatg tagt
//
LOCUS       DASVEV010000089         7997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1583172, whole genome shotgun sequence.
ACCESSION   DASVEV010000089 DASVEV010000000
VERSION     DASVEV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583172"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..78)
                     /locus_tag="VF222_04320"
     tRNA            complement(4..78)
                     /locus_tag="VF222_04320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Val,seq:tac)
     gene            360..1682
                     /locus_tag="VF222_04325"
     CDS             360..1682
                     /locus_tag="VF222_04325"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7141683.1"
                     /translation="MSAKESYNKIFELMKSHHDWFANSIPLIASENIPSTAVREAIVS
                     DFGNRYAEGWPGERVYAGCTFIDQVEIICNELARKVFNAEFADCRATSGVVANLAIYS
                     GFTLPGETMIATSIPNGGHISHGRKEHFGTAGHVCGLNIEFFPFSKEEMTIDVDLTKK
                     KILDLINNGKPPKMGMFGGSLFLFPHPVKELVDFMHDHKMFINYDGAHVAGLIAGGEF
                     QDPLREGVDSMTMSSHKTLWGPQGGIILSLERYAETIKKSIFPGNTSSHHLHHVAGKA
                     IALAEVLEFGKDYAKQVIKNAKKLAEILADYGFTVLGEKRGYTQSHQIAVDVSKYNDG
                     GTIEEDLEKSNIILNRQLLPGDIQAGRHYMHPSGIRIGANEITRLGMKESEMIEIANF
                     IKRVVIDKEDTNNIKRDVTEFKKQYQKLNYVFDGQLEAYKYISLVQVK"
     gene            complement(1753..5391)
                     /locus_tag="VF222_04330"
     CDS             complement(1753..5391)
                     /locus_tag="VF222_04330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HEX7141684.1"
                     /translation="MGKEIFRYYSINKEMNDQYHFLDETSIQELSNLKRPLFYNNYHD
                     NLLNLLNKTYLIASDARIQGIDVSEKIESRIVYDLADRVAKMHDINITNRLRNLLSET
                     TKEKAALKISEEIALGKYSNDDLTTRLDNAVRVSLAIVTEGVTVAPLQGISDVQIKNN
                     SDGSKYLSISFAGPIRSAGGTEAALTMLIADHVRKSVGLSKYQANSFDDETGRFVEEL
                     RIYEREVGSFQFKILDEDVIKCISNLPVELDGVDTDPVEIVGHRAMKRIKTDRVRGGA
                     LRVMNDGLIGRSRKLLKIIDSLGLDGWDWLNELKGAIQKEGSSEDDTVHHRMSEVITG
                     RPVLSMSNKLGGFRLRYGRCYNTGFSTIGIHPTIPILLNYAIVVGTQIKIDIPGKAAT
                     IALVDSIEPPLVKLDDGTVTYVTSIEHAHQIQNRIDTILYLGDILVSYGDFLENNAKL
                     FPASYVEEIWGLELQMKIKQISSNNSYSTEKKYVNEILEYIDKQRLFELANNPIEIIP
                     TLKEAFLISKILSIPLHPKYSFYWESISVDEIILLRNEFLKKSDIDKDVDLIFKITKA
                     PTNQYYYESFSIEYNEILKDILERLGITHSIKNYNNNPCIELSELNQINSLFILLCNP
                     SINNESSNEISSIDYIIRISGLLLKPKFSSSIAVRVGRPEKAAERKMKPPVHVLFPVG
                     SKGGSTRDLIKASKEEILYTEIANRYCKTCNLPSVGTKCRNCLSETPLNNICGICREP
                     INSGNNSKKEVTTRCTKCRREGKTYSPSKYPLKKMLKEAEKTLEIHSKEPLKGVKILM
                     GKNKSAEPLEKGILRQNYDLYAFKDGTIRYDATNGPLTHFKPEWIMTNIDKLRNLGYT
                     KDFYGKDLSSDDQLLEIFIQDVILPIDTANHLLRLSRYIDKELLKLYKLEPFYNASSI
                     DDLIGHIIVGLAPHTSVGIIGRIIGYTNSQVCLASPMWHSAKRRDCDGDADSIMLLMD
                     IFLNFSKEFLPDKIGGLMDAPLLIQPIVLPHEVQRQALNIDVTSNYPLQLYEKSLEYT
                     KAENLSNKIDILKDRIGSELQFHNYYFTHHTNLLTTEEKRSAYSRLTTMNEKLDMQIS
                     TAKLINAVVPDEVASMVLTTHIIPDIMGNMRSYSSQAFRCTKCGEKYRRIPLAGKCLG
                     CGNNLIQTVTRNSVEKYVYIALDIRNKFKMNDYLSSRIESLVMELNYIFKEKEIQKNP
                     QSSIFDYL"
     gene            complement(5346..6083)
                     /locus_tag="VF222_04335"
     CDS             complement(5346..6083)
                     /locus_tag="VF222_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEX7141685.1"
                     /translation="MNTIETYILNEIKRYGTLCFPLIDSENPNDVISIAKTVERLGAS
                     AILIGGSSIIDQIELSKIISQIKTTITIPVILFPGNVTGVSPNADAILFMSLLNSENP
                     YFITGAQALAAIGVKKSKLEALPTGYLIIGEGTTAWFVGQAKGIPFHKPNLAIMYSLA
                     AQYLGMRFLYLEAGSGANQHISPEMVKSVRKYYEGVLIVGGGIRNSEIAKHLANAGAD
                     IIVVGTMIENDNNWEKKFSDIIQLIRK"
     gene            6274..6666
                     /locus_tag="VF222_04340"
     CDS             6274..6666
                     /locus_tag="VF222_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141686.1"
                     /translation="MYSTDIKTELKFTDLLDLLIEKNLFTKKQISIIYNKINTHVSNV
                     SNNQMSAGAYYRQIKQCRKKYDQLIYTILLFRLLNLIDNNTMDTLELLVKQLDKISRR
                     DLNHDTTENVHNDIIIVIEEILRKINKI"
     gene            6725..7435
                     /locus_tag="VF222_04345"
     CDS             6725..7435
                     /locus_tag="VF222_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7141687.1"
                     /translation="MDIDIIYFIVTGIFFIVGFLLSHLLKIRKKIVSQTVPLQRNDNL
                     EILEVIENKKQLQIDNIILKLNDIQIRLDLLESKVSQSKNQSPYEHMVDNTIKNITEN
                     NYNTSHHNDINDITQQSIPKKSQIEKAKNKTVVLNDDKHNATEHYILKIILKESLTSN
                     EIKNAIGRTREHTSRLMKKLYELQLVDRDITAKPFKYKLTEQGKKYIGEQVENKEINQ
                     ENPLNSSYTNDSLIDLTK"
BASE COUNT         2842 a         1157 c         1144 g         2854 t
ORIGIN      
        1 gaatggaggg gatgggattt gaacccatga ccacctgcgt gtaaggcagg tatcctaacc
       61 agtctagaca acccctccgt tattgatctt tgattcattg ttataactat tatattaatt
      121 tatgattaag attttttcat agattgtgga atcatattga tatctataat aataattata
      181 attttaatgt aaatttaata attttaaaag ttattccttc tatcttttat tatttttatt
      241 taacaaaatt ctgatattat ctttggatga atatagtgat ttaataatta aaaatgattt
      301 caatattgtt tttaattaca acatttttaa attaggaagg aaattcaaaa actatactaa
      361 tgagtgcaaa agaatcctat aataaaatct ttgagttaat gaaaagccat catgattggt
      421 ttgctaattc cattccatta attgctagtg agaatattcc tagtacagca gtaagagagg
      481 caatagtatc tgattttgga aatagatatg ctgaaggatg gcccggtgaa agagtatatg
      541 ctggttgtac ttttattgat caagttgaaa taatctgtaa tgaacttgct aggaaagttt
      601 ttaatgcaga atttgccgat tgccgtgcaa cctcaggagt agttgcaaat ctagcaatat
      661 attcaggatt tactcttcca ggagaaacaa tgatagctac atctatccca aatggaggac
      721 atatttctca tggaaggaaa gaacattttg gcacagcagg tcatgtgtgt ggattaaata
      781 ttgaattctt ccctttttca aaagaagaaa tgacaattga tgttgatttg actaaaaaaa
      841 agattttgga tttgattaat aatggtaaac ctccaaaaat gggtatgttt ggaggaagct
      901 tattcctttt tccacatcct gtaaaggaat tagtcgattt tatgcatgat cataagatgt
      961 ttattaatta tgatggggca cacgttgcag gattaatagc aggaggagaa ttccaagatc
     1021 ccttacgaga aggagtagat tctatgacta tgagctctca taagacatta tggggtcctc
     1081 agggaggaat tatactttca ttagaaagat atgcagagac aataaaaaaa tcaatatttc
     1141 ctggtaatac aagtagtcat cacttacatc atgtagctgg taaagcaata gcattagcag
     1201 aagtgttgga attcggaaag gattacgcta agcaagtcat aaaaaatgct aaaaaattag
     1261 cagaaatttt ggcagattat ggatttactg tgttaggaga aaaaagaggt tatacacaat
     1321 cacatcagat tgcagtagat gtttcaaaat ataatgatgg tggaactatt gaagaggatt
     1381 tggaaaaatc taatataatt cttaatagac aattacttcc aggagatatc caggcaggaa
     1441 gacattatat gcatcccagc ggtataagaa taggggctaa tgaaataaca agattaggaa
     1501 tgaaagaaag tgaaatgata gaaatagcta attttataaa aagagttgtt attgataaag
     1561 aagataccaa taatataaaa agagatgtta ctgaatttaa gaaacaatat caaaaactaa
     1621 attatgtttt tgatggtcaa cttgaagctt ataaatatat cagcctagta caagtcaagt
     1681 aatcattcta aataaataaa taaataaatt tataaattta taaatttaga gttacaattt
     1741 aattttattg atctataaat aatcaaaaat agatgattga ggattttttt gtatttcttt
     1801 ttctttaaaa atatagttta attccataac tagtgattca attctacttg aaagataatc
     1861 attcattttg aatttattac gtatatctaa tgcaatatat acatatttct ctacagaatt
     1921 tcttgtaacc gtttgaatca aattattacc acatccaaga catttacctg ctagtggtat
     1981 tctacgatat ttttctccac atttagtaca tctaaatgct tgtgatgaat atgaacgcat
     2041 atttcccata atatcaggta tgatgtgagt ggtaagaacc atggatgcga cttcatctgg
     2101 aacaactgca ttaatcaatt ttgctgttga aatctgcata tctaattttt cattcatggt
     2161 tgttagtcta gaatatgcac tacgtttttc ttcagtggtt aataagtttg tatgatgagt
     2221 aaaataataa ttatgaaatt gtaattcaga accaattcta tctttaagaa tatcaatctt
     2281 gtttgaaaga ttttctgctt ttgtatactc tagagatttt tcatataatt gtaatggata
     2341 attcgaagta acatcaatat tgagagcttg tctttgtacc tcgtgaggta aaacaatagg
     2401 ttggatcaat aaaggtgcat ccatcaaccc tccaatttta tctggaagaa attctttaga
     2461 aaaatttaag aatatatcca ttagtaacat aatagaatct gcatcaccat cacaatccct
     2521 acgtttagca gaatgccaca ttggagaagc taaacagact tgtgaattag tatatcctat
     2581 aatccttcca atgattccta cggatgtatg aggagcaagt cctactatta tatggcctat
     2641 taaatcatct atagatgagg cattataaaa tggttcaagc ttatataatt ttaatagttc
     2701 tttgtctata tatcttgata gtcgaagtaa atgatttgca gtatctatag gtaatatcac
     2761 atcttgaatg aaaatttcta ataactgatc atcactagac aaatcttttc cataaaaatc
     2821 tttagtataa cctaggttcc ttagtttatc tatatttgtc atgatccact caggtttaaa
     2881 atgagttaat ggtccgttag ttgcatcata acgtattgtc ccatctttga aagcatataa
     2941 atcataattt tgccttaaaa ttcctttttc taatggttca gcggatttat tttttcccat
     3001 caaaatcttt acacctttta atggttcttt agagtgtatt tctagagttt tttccgcttc
     3061 ttttaacatt tttttaagtg gatatttcga tggagaatat gtttttcctt cccttctgca
     3121 tttagtgcat ctagtagtta cttccttttt actattattt ccagaattaa ttggttctct
     3181 acagattcca caaatattat tcagaggagt ctcagataaa cagtttctac atttagtacc
     3241 aactgatggt agattacaag ttttacaata tctattagct atttctgtat aaagaatttc
     3301 ttcttttgat gctttaatta aatctcgtgt agaacctcct ttacttccta ctggaaataa
     3361 gacatgtact ggcggcttca tttttctttc ggctgctttt tctggccttc caacacgtac
     3421 agctattgat gaagaaaatt ttggttttag taaaagtcca gatattctta ttatataatc
     3481 aattgaagaa atttcatttg atgattcatt atttattgat ggattacaaa gtaaaataaa
     3541 caaagagttt atttgattta attctgacaa ttctatgcat ggattattat tataattttt
     3601 tattgaatgt gttataccta aacgttctag tatatctttt aatatttcat tatattcaat
     3661 tgaaaatgat tcataataat actgattagt cggggctttt gtaatcttaa aaattaaatc
     3721 aacatccttg tcaatgtctg atttttttaa gaattcattt cttaataaaa taatttcatc
     3781 cactgatatt gattcccaat agaaagaata tttaggatgt aaaggaatac tcaatatttt
     3841 actaattaag aatgcttctt tcagtgttgg aattatttca ataggattat ttgctaattc
     3901 aaataatcgt tgtttgtcaa tatattctaa aatttcattg acatattttt tctcagtgga
     3961 ataactatta tttgaagaaa tctgctttat tttcatttgt aattctaatc cccatatctc
     4021 ttcaacatag gatgcaggaa ataattttgc attattttct aagaaatcac cataactaac
     4081 taaaatatct cccaaataaa ggatagtatc aattctattt tgaatttgat gggcatgttc
     4141 tatagatgta acataagtta cagttccgtc gtctaatttt actaaaggag gttctattga
     4201 gtcaactaat gcgattgttg ctgcctttcc aggaatatca attttaattt gagttcccac
     4261 tactattgca taatttaata atataggaat ggttggatga atccctatcg tggaaaatcc
     4321 tgtattataa catctaccat atcttaacct aaatccacca agtttattag acattgacaa
     4381 aactggtctg ccagttatta cttctgacat cctatggtgt actgtatcgt cttcactaga
     4441 gccttctttt tgtattgctc ctttaagttc gttaagccaa tcccatccat ctaatcctaa
     4501 actatctatt atttttaata attttctact tcttccaatt aaaccatcat tcataactct
     4561 taaagcaccg ccacggactc tgtctgtctt gattcttttc attgctctat gaccaacaat
     4621 ttctacagga tcagtatcta ctccgtctaa ttctactggt aaattactaa tacattttat
     4681 tacatcctca tctaaaattt taaattgaaa acttccaact tctctttcat atatacgtaa
     4741 ttcttccaca aatcgaccag tctcatcatc aaaggagttg gcctgatatt tacttaaccc
     4801 aacggacttt cttacatggt ctgcaatcag cattgttagg gctgcttcag ttcctccagc
     4861 agatctaatt ggtccggcaa atgatattga aagatattta cttccatcag aattattctt
     4921 gatttgtaca tcagatatcc cttgtaaagg tgcaacagtt actccttctg ttactatagc
     4981 aagacttacc ctaactgcat tatctaatcg agttgttaaa tcatcattgc tgtatttacc
     5041 taatgcaatt tcttcggata tctttaatgc tgccttttct ttagttgttt ctgataataa
     5101 atttcttaat cgatttgtaa tgttaatatc atgcatttta gctactctat cagctaaatc
     5161 atagactatt cttgactcaa ttttttcaga aacatcaata ccttgtatcc gtgcatcact
     5221 ggctattaga taagttttat ttaataagtt taagagatta tcatgataat tattataaaa
     5281 tagcggtctt tttaaattac ttaactcttg aattgaagtt tcatctaaaa aatgatattg
     5341 atcgttcatt tccttattaa ttgaataata tcggaaaatt tcttttccca attattatca
     5401 ttttcaatca ttgttcccac aactataata tcggcaccag cgtttgctaa gtgtttggca
     5461 atttcagaat ttcgaatacc tccacctaca attaaaacac cttcataata tttacgcact
     5521 gatttgacca tttctggtga aatatgctga ttggctcctg aacctgcctc tagatataag
     5581 aatcgcattc caagatattg tgctgctagt gaatacatta ttgccaagtt aggtttatga
     5641 aaaggaatgc cctttgcttg tccaacaaac caggcggttg ttccttcacc aattatcagg
     5701 tatcccgtag gcaatgcttc taacttggat ttctttactc caatagcagc taatgcctgt
     5761 gcacctgtga taaaataagg attttcggaa ttaagtagtg acataaataa aatagcatca
     5821 gcattagggg aaacaccagt gacatttcca ggaaataata tcacagggat agtgatagtt
     5881 gtttttattt gtgatattat ttttgataat tcaatttgat ctattatgga tgaaccaccg
     5941 attaaaattg ctgaagcacc taatcgttcg actgtttttg ctattgaaat gacatcgttt
     6001 gggttctctg aatcaattaa tggaaaacac aaagtaccat atcgttttat ctcatttaaa
     6061 atataagttt ctatagtatt caattactgt ggaaccatat taaagtatat taactcctaa
     6121 aaaatatttt tggtttttat ttattatata tttataaaac ttttaccttt aagtattaaa
     6181 taagtgatat aaaataaatg gataaaattt agacttttgt tattctatat aaatcgtggt
     6241 tactttttat acagaatcaa gtactataca aatatgtata gtactgatat aaagactgaa
     6301 ttaaaattta ctgatctttt ggacttactt attgaaaaaa atttatttac aaagaaacaa
     6361 atatcaataa tttataataa aatcaatact catgtttcta atgtctctaa taatcaaatg
     6421 agtgctggtg cttattatag acagattaag caatgtagaa aaaaatatga tcaactcatc
     6481 tacacaatac ttttgtttag attactaaat ttgatagaca acaatacaat ggatacatta
     6541 gaattgttag ttaaacaact cgacaaaata tcacgacgtg atcttaatca cgatactact
     6601 gaaaatgttc acaatgatat catcatagtg atagaggaaa ttcttagaaa aattaataaa
     6661 atataagaag gaaatatgca tagaagagat aaagataaat attttgtgat atatgtgata
     6721 ttatttggat atagatataa tttactttat tgttacagga atctttttta tagttggttt
     6781 tttactttct catttgctaa aaatacgaaa aaaaatagtc agtcaaacag taccattaca
     6841 aaggaatgat aatttagaaa ttctagaggt aatagagaat aaaaaacaat tacagattga
     6901 taacattata ctcaaactta atgatatcca aatcagatta gatttacttg aatctaaagt
     6961 atcgcaatca aaaaatcagt ctccatacga acatatggta gataatacaa taaaaaatat
     7021 cacagaaaac aattataata catcacatca taatgatatc aatgacatca cacaacaatc
     7081 tatacctaaa aaatcacaaa ttgaaaaagc aaaaaacaaa accgtagtat taaatgatga
     7141 taaacataat gctactgaac attatatctt aaaaataatt ttaaaagaat cattaacatc
     7201 taatgaaata aaaaatgcca ttggtagaac acgagaacat acgtcacgac taatgaaaaa
     7261 actctatgaa cttcaattag tagataggga tataactgca aagccattta aatataaact
     7321 aacagaacaa ggaaaaaagt atattggtga acaagtagaa aataaagaaa ttaatcaaga
     7381 aaatccgcta aattcatcat atactaatga ctctttaata gatttaacca agtagaaatc
     7441 attactatta acattattaa ctattaacat ttcttataaa tatcaagtaa aagaattgtt
     7501 aacatcctaa tacgcataat aatgaagtat tgttaataca aaaaagtcat aagagaacta
     7561 attattaatc tgcagtagaa ataaaggggt caaattaatc attgttaaca aacttaacat
     7621 aattctaact ctgcagtaga aataaagggg tcaaattaat ccagttttta ataaagtagg
     7681 tgatatactt attattgtta atattttgtt gttattagtt taacaacact tgtccccctt
     7741 catttctttt gcaaatataa tataaaaata agattattat aagttaaata ttactattaa
     7801 aatagtttaa aatatttgat ttatgggttt aaattgatat gttaataaca tgttaaaata
     7861 taataacatg ttaatataaa tttgaaatgc aatagaaagt aaggggtaag gggataattt
     7921 tatatggtga gttatttttg agcgatgata ttttagataa tatatttgaa aatgcagtaa
     7981 aaggaaactc gttaatc
//
LOCUS       DASVEV010000090        14307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_805780, whole genome shotgun sequence.
ACCESSION   DASVEV010000090 DASVEV010000000
VERSION     DASVEV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805780"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..973)
                     /locus_tag="VF222_04350"
     CDS             complement(104..973)
                     /locus_tag="VF222_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7141688.1"
                     /translation="MIDNVPSSQAQKFKDVEIIVNQLDNNGTYLLVGKGGNIGLSVGD
                     DGVLLIDSQFEQLTGKILSAIYNKITDKPVIFVINTHWHQDHTGGNENLVNNGSIVIA
                     HENVRKRLSTEQFVDFLNREYKPAPINALPTITYNDSITFYFGDDKIDLYHLPNAHTD
                     GDSIIYFNKRNVIHTGDVYVNGRYPFIDHSSGGSIDGIITGIEKIISIIDNETKIIPG
                     HGLLSNLDELQDYLHMLKDIRQQVETMVKNKYNLEEIIKSDITSDYDGLYSDSFINSG
                     DFLAFVYNDIKHK"
     gene            2050..2433
                     /locus_tag="VF222_04355"
     CDS             2050..2433
                     /locus_tag="VF222_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141689.1"
                     /translation="MTKRILLVDDEADIIFTMKDILEEEGFKVDTFNDPTIALESYRS
                     NLYDLIILDIKMPKMDGFQLYSKLKEKDPQVKICFLTASEMFYEKFRKTRLVFTKTIG
                     EDYFIQKPINTEDLIRRLTSIMDKK"
     gene            complement(2708..3679)
                     /locus_tag="VF222_04360"
     CDS             complement(2708..3679)
                     /locus_tag="VF222_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX7141690.1"
                     /translation="MCLTAILFGEGLSKYGFGRLHPWNNERIYDFWSKFQQMGLDKTP
                     EILIEEPELALEESIRSFHDKDYVDLVKKAGKTGNGYLDTGDTPAFRGVFEASSYVVG
                     STLKAFDIVIRKKKGIEHAFNPIGGLHHSKRTAAAGFCIFNDIGIAIIEARRKYGIKK
                     IAYIDIDAHHGDGVYYEFEDDPLLYIADIHEDGKFLYPGTGSEWETGTGDARGTKLNI
                     PLNPDSTDQDFIASFKRVEDFIDNKAKPELIILQCGADSLKHDPLTHLNLTHKSHKYA
                     ASKLHSLAHKHSNGRIVALGGGGYNKINIGDAWTAVVKAFIEKLDAN"
     gene            3890..4132
                     /locus_tag="VF222_04365"
     CDS             3890..4132
                     /locus_tag="VF222_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141691.1"
                     /translation="MKASILVIIMITLMIGVIAISGTQQTAFAENKAKEYCSDYGGEW
                     DNGKCKIEDDEDKADYEDDVCDDAEDSKKYKKICNP"
     gene            4350..4607
                     /locus_tag="VF222_04370"
     CDS             4350..4607
                     /locus_tag="VF222_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141692.1"
                     /translation="MKYRSRTEIVAMILDAANGGATKTRIMYKAFLSYAQLKEYLSVL
                     VENNLIEYLDGIQVYKTTEKGLKFLKMHSEIGELLPTTIKE"
     gene            5251..5727
                     /locus_tag="VF222_04375"
     CDS             5251..5727
                     /locus_tag="VF222_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7141693.1"
                     /translation="MSNANKMDKPRNPIPTIDIILSENSNSNKVLLIKRNKDPFKDYF
                     SLPGGFVNEGEKVEDAVRREAEEELLVKVEPINILGVYSDPNRDPRGHIMSITFIAKI
                     IKGNLKEGDGVAELRWVEINKLENIKLGFDHSKILSDYETWLKNKETYWSSKENIG"
     gene            complement(7428..7904)
                     /locus_tag="VF222_04380"
     CDS             complement(7428..7904)
                     /locus_tag="VF222_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7141694.1"
                     /translation="MSTSNTKKIKFSKILVPIDGSPSSLTSAIYATDFASTYDAEIIL
                     LSVVPSQIKHGDSSGVFGMVTPHHLDQYKKEAEKWFNKIIDDLKNQGYNINKITTDVI
                     TTPMSIVGAIVDYAEKENTDMIILGKSGSTGFKRILLGSTASGVVTYAHCHVFVVR"
     gene            complement(8321..8461)
                     /locus_tag="VF222_04385"
     CDS             complement(8321..8461)
                     /locus_tag="VF222_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141695.1"
                     /translation="MKKDYILMGLFDRFRKKKKDSEDLGGKTADIYDEQRKNPEKEPV
                     SN"
     gene            8973..9188
                     /locus_tag="VF222_04390"
     CDS             8973..9188
                     /locus_tag="VF222_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7141696.1"
                     /translation="MTHNIANITELIGSSDKGWQEAAKVALDEATKTIRGITGIEVTS
                     KTATVDPNTGNITQYKTTIKVAFGVER"
     gene            complement(9710..9952)
                     /locus_tag="VF222_04395"
     CDS             complement(9710..9952)
                     /locus_tag="VF222_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141697.1"
                     /translation="MRYALNIQAAVLYGMLKPCIISGIAGSNIVSENITTKRVLLSKT
                     RMNRAERLWITAVFVVVFCFSISFRRLFFSPSILEI"
     gene            9894..11270
                     /locus_tag="VF222_04400"
     CDS             9894..11270
                     /locus_tag="VF222_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7141698.1"
                     /translation="MQGFNIPYSTAAWIFSAYLIVAAVMTPIAGKFSDIYGSKKVLLI
                     LLVLYIIGVVAGGFSDNIFFLLASRVIQGVGLAAVPVAFSIIRDSFPPEKLAMPVGVF
                     SSAASGGSVVGLLIGANIIQNFGWHATFFFIAPAAVIVTLLIARFVPNDDKPKPKQYL
                     PSSGNDNNSNKKHATNGRKSMIDIKGAISLSATITSFLIALTFIENSNSLKNSMEIAG
                     ISFIASATSLILFIFIEKRTKMPLIDLWLLKQRILLPSYIIMIVTGTTIFLVYPTIVQ
                     LVRSPNPAGFGGDAVYAAYIQLPFMLVFLLFSSATSYIISKLGKVMPTVLGGTISIAG
                     AIGLLIFHSTGLVVSINLTIIAIGLALTITATWNLIVSSAPKEFMGISTGIGALLSFI
                     GMAIGPALAGTYMHTFHMSIDGIAGVYPSPESFNMIFFTAALLSTVSLTFSLLLKRRT
                     SRNTEIIT"
     gene            13204..>14307
                     /locus_tag="VF222_04405"
     CDS             13204..>14307
                     /locus_tag="VF222_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141699.1"
                     /translation="MPLYDCPHCKLTSNRKYNMKKHIERKHPGSEIPNNLLSVRGTNL
                     KYGRYVCLHCKLTSNRRYNMKKHIERKHPGSEIPNNLLSASYNYNNNGYIHRGPQNLD
                     VYFRAKHKSSVPFDATSYINNPFDEDILPILDTHKSNKNSHSIFWEMLRSVQLLNDLP
                     TEYILPKRDFYLYNPMLNVNFSNHYKNQPRPSVDLISYNKEILFRIQRCNNCTTVILV
                     KFFGFDNIKPIIDCRCDNICRSQKQNKEISYLTIKEFLIKSIVSFIDKEMYSKIYLKC
                     IKIPSNIYTKLIVEKKPFFLNDKEVNIPSWMLTPIMHKEFVDLGVIDDDNWAYRLTNN
                     NEKNIEITKLEMMEFINYGDSTFGLFRFHKDNIS"
BASE COUNT         5171 a         2023 c         1980 g         5133 t
ORIGIN      
        1 tttagataag ttattgaatg tgaatacata atgtaatatt taaatgtatt taaataactt
       61 caaatgaaga aatcttgttt gataattttt ataacaataa aaattattta tgttttatat
      121 cattatagac aaatgctaaa aaatctcctg aattaataaa actatcagaa tataatccat
      181 catagtcaga tgttatatcg cttttgatta tttcctcaag attatattta ttttttacca
      241 ttgtctctac ttgttgtctt atatctttta acatatgaag atagtcttgt aattcatcaa
      301 gatttgataa taatccatga cctggaataa tctttgtttc attatctatt attgaaatta
      361 ttttttcaat tcctgttatg ataccatcta ttgaaccacc actagaatga tctataaatg
      421 gataccgtcc gttaacataa acatctcctg tgtgaattac atttcttttg ttaaaataaa
      481 taattgaatc accatcagta tgtgcatttg gaagatgata taaatcaatt ttatcatcac
      541 caaaataaaa agtaatagaa tcgttgtaag taatagtagg caatgcatta attggagctg
      601 gtttatattc tctattcaaa aaatcaacaa attgttcagt gcttaagcgc ttcctaacat
      661 tttcatgagc tattacaata gatccgttat taacgagatt ttcattacct ccggtatgat
      721 cttgatgcca gtgagtatta ataacaaata ttactggttt atcggtaatc ttattataaa
      781 ttgcagatag tattttgcca gtcaattgtt caaattgtga atcgattagt aagactccat
      841 cgtcaccaac tgacaatcct atattacctc ctttcccaac cagcaaatag gttccattat
      901 tatccaattg attgacaata atttctacat ctttgaactt ttgggcttga cttgaaggaa
      961 cattgtctat catgaataac atacacaata ccaaaataga ataagaaaga gggaatttaa
     1021 taattcttga atatccatct atatttttaa atacaaaaaa gaacttcata aaaaaattat
     1081 aaatgtgatg gaataaatag ttttgataaa aatgaactac acaaatgaag aatttattac
     1141 taatatttta attaaaaaaa taaatacact aattagattt tgtataacat aattttatta
     1201 tactttgatc caaagtaaaa gtattattta ctctgataat acattaataa tacaacctgt
     1261 aatctaattc aaatacatag ttattaagtt tataataatt actatattga atagtaaatc
     1321 tttaagaaga taatcgactt aaattaaaat attcaaattt tattttgttt tttttatatc
     1381 tctcagtatt gctttttcta tttgtcactt gtattaaaga tgatattatc ctcaccatct
     1441 aaggtgaacc atatcggaac tacatgcggt gtctcatttt tgttaattgt tccgagttct
     1501 ctataaaagt tcctggcaaa agaaaacttc tgatttcata tatatttatt attttaaaaa
     1561 aaccatttat gcactaacaa atttgtgtca aatcatttga ttatctcccc gtttgcaagc
     1621 taacgaaaaa gttttagtat acacaaaacc taaattcaaa cgagaattat tctagataaa
     1681 gcacaacatg ctatactact actccagaga atgggtatta acaccttgca tgaacattct
     1741 ccggatagtg tggatttaat ccaaactaac ttttggatca tatttattat agtttgttat
     1801 aaattattct ctatggttat tctataacaa ataaaattgt aatgaactac aaaagtgtat
     1861 caaaatcatt tgactcttaa tattttaata ctcaaatact gttttttatt catcttagaa
     1921 ctaagaagtt aatcaaggaa aaatacggac aaaaaatatt ccaatggtag aggatctact
     1981 ttttatttta tgcttttgat aattaataga gataggggtg taatcaatcg gaagtgatgt
     2041 ctaataacaa tgactaaaag aattttatta gtagatgatg aagcggatat catttttacg
     2101 atgaaggata ttttagaaga agagggattt aaagtagata catttaatga tcctactata
     2161 gctttagaat cttatagaag caacttatat gatctaataa ttctcgatat aaagatgcca
     2221 aaaatggatg gatttcaatt atatagtaaa ttaaaagaaa aagaccctca agtaaaaatt
     2281 tgctttttaa cggctagtga aatgttctat gaaaaattta gaaaaacacg attagtcttt
     2341 actaaaacaa ttggtgaaga ctactttatt caaaagccaa tcaatactga agatctaatt
     2401 agaaggttaa cttctataat ggataaaaaa tgaggatgag aaaatagaag tcttttatca
     2461 aataacatta agtttaccgt tacctatgca atagatgcaa tcgcaaaaga aataaaataa
     2521 taagttataa aacagattaa tacaagttta cagatcccat gtcatatccg aagctctcaa
     2581 agcttcaatg ttagttattg aactcattca ttcataagac tcaaatatga attggtaaaa
     2641 gttatctcta aaataaattc atccagactt caattgtttt tctatctttt ttatatattc
     2701 actaacatta attagcgtct aatttttcaa taaaagcttt tactactgca gtccatgcat
     2761 caccaatatt tattttatta taacctcctc caccaagagc aactattctt ccattactat
     2821 gcttgtgagc aagactgtgt aatttactag ctgcatattt atgactctta tgggtcaaat
     2881 ttaaatgagt taatggatcg tgttttagtg aatctgcacc gcattgtaag ataattaact
     2941 ctggttttgc tttgttatca ataaaatctt ctactctttt aaaagatgca ataaaatctt
     3001 gatctgttga atcaggattt aatggaatat ttaatttcgt acctctagca tcacctgtac
     3061 cagtttccca ttctgatccc gtaccaggat agagaaactt tccgtcttca tggatgtctg
     3121 caatgtatag aagaggatcg tcctcaaatt cataatagac accatcccca tggtgtgcgt
     3181 ctatatcaat gtaggctatc ttcttaatac catactttct tctagcttcg attatagcaa
     3241 ttccaatgtc attaaaaata caaaaccccg ctgcagcagt tctttttgaa tgatgtagtc
     3301 caccaatagg attaaatgca tgttctatcc cttttttttt tctaattact atatcaaatg
     3361 cttttaaagt tgatcctaca acgtacgaag atgcttcaaa tacccctcta aaagcaggag
     3421 tatcacccgt atctaaatat ccgttaccag tttttccagc cttcttaaca aggtccacat
     3481 aatctttgtc gtgaaaggat cgtatggatt cttccaaagc caattctggt tcttcaatca
     3541 aaatttctgg agttttatct aaacccattt gctggaattt ggaccaaaaa tcatagattc
     3601 tttcattgtt ccaaggatgt aatcttccga atccatattt tgagagccct tcaccaaata
     3661 atatagctgt caaacacatt aacaaataat aaacaacaaa cgcaataaaa gaatcctcaa
     3721 acaaagatag ttattgtgta atataatcat aaacttttta ggcgccgaaa atattaatat
     3781 gattatgaag aaagttacat tacaaggatt agaaagacat tataattcat aaaaaattat
     3841 tttattttgt caaaaaaagt taattatatc tacatatttc taaaaataga tgaaagcatc
     3901 aatattagta ataataatga taacactgat gataggtgta atagcaattt caggaaccca
     3961 acaaacagct tttgcagaaa acaaggctaa agagtattgt tctgattatg gtggagaatg
     4021 ggataatggc aaatgtaaaa ttgaagatga tgaagataaa gcagattatg aggatgatgt
     4081 atgcgatgat gccgaagatt ctaaaaaata taaaaaaata tgtaaccctt gaatgaatta
     4141 aaattaatta attgacaact cctattattt ttgaataaat acattattgt tgtgattttg
     4201 gactatttag tacctttttt tcaaaattat tgtaaatata agtaacctat tatcatttga
     4261 gaaagtatca agaagccaaa attgatatat actatggctt ccaaattata ttagtataca
     4321 tttatgtatt taaggatagt aatataaata tgaaatacag aagtagaaca gaaatagtag
     4381 caatgatact agacgctgca aatggaggag caaccaagac aagaattatg tacaaggctt
     4441 ttttaagtta tgctcaatta aaagaatatc tttctgtttt agttgaaaat aacctaatag
     4501 aatatcttga tggaattcaa gtctataaga ctacagaaaa aggattaaaa tttctaaaaa
     4561 tgcatagtga aattggagaa ttattaccaa ctactataaa agaataataa ttaactctca
     4621 gtattgatag aatttattta tactaaagat tctaatcaat tgctatatat tttatctaaa
     4681 ttgatctaaa atcattacac aatttagaaa ggacttttta attttgattt aatcaactct
     4741 gcttgtcttt ttaaatcttc ttcttcatgg atagtttcaa aaattttctg tagttactag
     4801 aaatttcaat aatttagtat aataatttat taatttatat tttattatgt tattgtctgg
     4861 tatacactgt aaaaacaaaa atacaaagat agtgctcaac aattagtttt gagttttctc
     4921 caaaaggtgt gttaatgacg ttaatagatt tgatctagtt aaaatcacat tttagtttct
     4981 tattttaaca aaatcactgt ttgtactatc taaaaattca aagtgcagtt atttcactgt
     5041 gttattttta ccattcaact acaaatctta tggtaaataa ttaaattaag ttattaaaca
     5101 tctaaatagc agtttacgga ttccaactac atttcaaact cgaagccctt atacttcaca
     5161 aaattaagtt ggaaaatata agcgtgtata gcctatattt tattttttga ctggataatc
     5221 ctattatagt ttttgttctc attaatttat atgagtaatg ccaataaaat ggataaacca
     5281 agaaatccca tcccaacaat agatattata ctatctgaaa attctaatag caataaggtc
     5341 ttattaataa aaaggaacaa agacccattc aaggattatt tctcattgcc aggaggattt
     5401 gttaatgaag gtgagaaagt agaagatgca gtaagaagag aggcagaaga ggaattatta
     5461 gttaaggtag aaccaattaa tattttagga gtttattctg atcctaatcg agatccaaga
     5521 ggacacatta tgtctataac ttttatagct aaaatcatta agggcaatct aaaggaggga
     5581 gatggtgttg cagagttaag atgggttgaa atcaacaaat tagaaaatat aaaacttggt
     5641 tttgaccatt caaaaatact atctgactat gagacatggc tgaagaataa agaaacatac
     5701 tggtcttcaa aggagaatat tgggtaaata ttttctactg ttaataaaaa aatttcaaag
     5761 attaagttta ctattgattt ttgttaaata tataatataa aagaaaatca aagtatgatt
     5821 gctattagac tgcaaagttg tgtccaaaaa cagttgaccc tcctccattt gtgcacctac
     5881 gaataagtta agtagtatat gaacaaattt taaaaatata attttagatg aattattttg
     5941 agctatttat cctgagaatg attattaaca ccttgcatga acattcttca aatagtgtgg
     6001 attgaaatcc aaactacctt tttgaatttt ataacgaatt gcataatcat aaataaaatg
     6061 ttctaattaa gaaaatataa aaaaaagaaa tttcttttag ggaactttat tatataaaca
     6121 tattatatta atatcattat agataatagt atttattaat ttcagtggtg aaatcagaga
     6181 gatctaaagt agaaactaat gctttaaatt cattatgtaa ccaagctgtt ttataatgag
     6241 aattagaagc tttctatgtt taattttagt tatagaaaaa gagagtaatt tctctagaat
     6301 aattatgtac taaggtaaac aatattgtta atatgttgat taaaatatta ggatatcttt
     6361 attctgatca aaattcaaaa aaaatacaac aatagcaata tatttatatt tatctgtcaa
     6421 attttgaggc gccgaaaaaa tagtattata aaaattaata gtcatttaaa gcctagcaat
     6481 cttaaataat ttatattata taactttgac ttttttttaa tcaatcgtaa atttatgtag
     6541 gatatccatt tcctggttta tattctataa atttggtttt acaattactg cattcataga
     6601 gaatcaaaac ctgattttca ttacgttcta gttgattgag atatactaat ttttgaatac
     6661 aagtggaaca tttcattttt acttactata atatctaaag aattagaata ttaaaagatg
     6721 tttatcttca taaactaaat ttaaacagta tacaaatttg gtaaccacga gttttatcgt
     6781 agtttgttta gctccaaaaa atttttatat caatttctta ttttatttat tatagtttcc
     6841 tacaagagca taagcataaa atctatcaga cgaagaagac aatcatcact atatagttgc
     6901 cctgacgtac ctgctaatct tgatatggaa tttcttacgt cagtaactaa aattgtactt
     6961 gttactattc tgcaaattac tagacaagtg ccatcgtttt agttggtatt tatttgcaat
     7021 ggattattca gatggatcac aaaattgaat tataatttct attagaaaat attgtatttt
     7081 taatcttaca cataatgaag atttataaaa catataatat tttgtaattt ctaaagatac
     7141 aattctccta ataacattag agcactaaaa cgattcattt ctttcctata taatatttga
     7201 ttagcaatat acattttatg tctatagcat attagaatac ttagttatat gtaatctaat
     7261 aaaattatat gttatttata ctcattgttt ggggaaatta ataaatgcta atatttagcg
     7321 taataatact ggtaataaca tttttacaca catctataac aaatattaga aagataactg
     7381 gtgtgattct atcaaggtat tttaatttaa gtagacagaa ttagaaatca ccttactaca
     7441 aatacatgac aatgagcata tgttactact cctgatgcag tactacccaa caatattctt
     7501 ttaaatccag tacttccact ctttcctaat ataatcatat cagtattttc tttttcagca
     7561 taatcaacta ttgctccaac gatagacatg ggagttgtta taacatcagt tgtaattttg
     7621 ttaatattat aaccttgatt tttaagatca tcaattattt tattaaacca tttttctgct
     7681 tcttttttat attgatcaag gtggtgggga gtaaccattc caaaaactcc tgatgaatct
     7741 ccatgtttta tttgtgatgg aacaacagat aaaagaatta tttcagcatc ataagtggat
     7801 gcgaaatcag ttgcatatat ggcagaagtc aatgaagatg gagatccatc aatcggaact
     7861 aagatttttg aaaatttaat tttttttgta ttggaagttg acaatcaatt cacctaaaca
     7921 ataaaaaacc tattaactat taatatttta caaatttctt taactataat ttaggttaag
     7981 atttaatcca gtcattataa tagattttca tagggctttg ttaacttttt taattatatt
     8041 ttatattacc tttaattgta aaataaataa ctattgtatc aaatattttc tataactaat
     8101 gcttaagttt atactaatgt cggatgaaaa ataaccgata aagtgttgtc aacattaaat
     8161 aatttttcga attatacggt tatatttata tactagaaat tttgtgccaa aaaacagtta
     8221 actcttcttc gtttgcgtgt ctactgaaag tttatgataa taacggagtg gtgcaatatt
     8281 ttaatatatt taggtataaa aaatgaaaaa taagttgaag ttagttactt actggttctt
     8341 tttccgggtt ttttcgttgt tcatcatata tatcagctgt ttttccgccc agatcttcac
     8401 tatctttttt cttttttcta aatctatcaa atagtcccat aagaatataa tctttcttca
     8461 tgtaaataaa gacatctatc tcagataaac taaagaaaag gataatagag aacgtttttt
     8521 gatgaagtaa aaggaggaaa atatactctg cctaccaagc cttatttgcc aatctgctcc
     8581 ttgagaggct acgcttatta aataacatgg ctatatgctg acaacagcca taggtaaaat
     8641 tcttccataa aaacaataga attaaagaaa atcatcgtta tttcttttaa ataaacaatt
     8701 aaaggggaat ataaaaatcg gaacacctca tcatagattc agattattag taaatcaaaa
     8761 accggttaaa aaataatcga atttatattc tgttctagta gtttaaaact tattaaagta
     8821 cataatacaa ttattgaaac tacttaatta atttccactt gacatttgat tgcatataac
     8881 aaattatagt tgtcaatttg tttgtttttt ataacatttc gagagattct tttattaata
     8941 acaattaata aacattaatc ccaataacaa atatgactca taatattgca aacataaccg
     9001 aacttattgg tagttcagat aaaggatggc aagaggcagc caaagttgcc ctagacgagg
     9061 caacaaagac gatacgtgga attacaggaa ttgaagttac aagtaagact gctacggtgg
     9121 atcctaatac aggtaatatt actcaatata aaactacaat aaaagtagca tttggtgtag
     9181 aacgttaaga atttgtaatt ttacataaaa tttttttatt aataatcttg ttatcttatt
     9241 tttattgtat atctataagg aactgaataa caaaactttt gattaatata aaattctttt
     9301 gtaaaaatga atgactcaat gcgataatcc caaagattat cgaactacaa aattgtgtca
     9361 aaatcatttg cttatcttaa gaacctagtt gtcaataaat ttttaattgt gttctgatag
     9421 tatttttgtt aaaagttatc taaaaaagat aataatccaa cttaaaagat caatatttat
     9481 attctcaacc ttatctgttt ttaaattatg tttaaaattt gttgaacatt tacatcgaaa
     9541 aattcatcaa atcaaaaaga aatatgtgtt ctctcttctt cttcttactg ctgaagagat
     9601 ggatcaagta aaacttgagg aatatgaaaa tacatgatta gaagtttgaa tgtgattaat
     9661 aaaataattc ttatacattt gtaaactcta gaatatatat ctcataaatt caaatttcta
     9721 gaatggatgg agaaaagaaa agacgacgaa aagagataga aaaacaaaac acaacaacaa
     9781 aaacagcagt aatccataat cgttcagcgc ggttcattct agttttactt agtagtactc
     9841 ttttggtggt gatgttctcc gaaactatgt tactgccggc tattccggat ataatgcaag
     9901 gttttaacat accatatagc actgcagctt ggatatttag tgcatatctc attgtagctg
     9961 ctgttatgac accaatcgca ggtaagtttt cagatattta tggtagtaag aaagtgctac
    10021 ttatcttgct tgtgctgtat ataataggtg ttgttgcagg aggattttcc gacaatatat
    10081 tttttcttct ggctagtaga gtaatccagg gcgtcggctt ggctgcggtt cctgtggctt
    10141 ttagtattat tagagattcg tttcctccag aaaaacttgc tatgcctgtg ggagtgttca
    10201 gttcagcagc gtcgggcggt tcagttgttg gattactgat aggtgcgaac ataatccaga
    10261 attttggatg gcatgccact ttcttcttca tagccccagc agcagtgatt gtaacgttat
    10321 tgattgccag atttgtccct aatgatgata agccaaagcc gaagcagtac ctaccgtcat
    10381 ctggaaacga taacaatagt aataaaaaac atgctaccaa tggtagaaag tccatgatag
    10441 atatcaaggg cgctatatca ctttcagcta cgataacatc ttttctgatt gcattaacat
    10501 tcatagaaaa tagtaactca ttaaaaaatt cgatggagat tgcaggaatt tcttttatcg
    10561 catcagcaac ttctttgatt ctctttatct ttatagaaaa aaggacaaag atgcccttga
    10621 ttgatttgtg gttgttaaaa caaagaatct tgttgccatc ttatatcata atgatagtta
    10681 ctgggaccac aatttttctg gtatatccta cgatagtgca attagtaaga agccctaacc
    10741 ctgctggttt cggtggagat gctgtatatg cggcttacat ccagcttcca tttatgttag
    10801 tatttttatt attctctagt gcaacatcct acattatatc taaactagga aaagtaatgc
    10861 ccacggtgtt aggaggtacg atcagcattg caggagccat tggtttgttg atattccact
    10921 ccacaggact cgtggtatct ataaatctca caataattgc gatcgggcta gcactgacaa
    10981 tcactgcaac gtggaacttg atcgtttctt cagctccaaa ggagtttatg ggaatatcga
    11041 caggaatagg agcactactt tcattcatag gaatggctat aggtcctgca ttagctggaa
    11101 catatatgca tacatttcat atgtcgatag atggtattgc tggtgtatac ccttcaccag
    11161 aatcgtttaa tatgatcttc tttacagctg cgttgctgtc aactgtgtcg ttgacttttt
    11221 ctttattgtt aaagagaaga acatcacgaa acacggaaat tattacataa ttaaggcaca
    11281 aatattaact aacgttatcc aaaacaatac ccattttatg ttgctatatc tttttaatct
    11341 aaattttatt tgaaaagaaa ataagttatt tctaatactt tataagaaaa taatcatatt
    11401 ctaggcttaa gtgctttatt actcatgtat ctaattaaat ttacttcctt taataatact
    11461 atatatgaag ctctgtaaat gataataata ttgtgtatcg tatatacagt ttttcatact
    11521 ccttaatttt ataattaaat gcatatatta catttaagat aaccatctcg atgttttaaa
    11581 agttgttgat atcaaatttt agtctaatat tttctttgtt tctactattt tgggttaata
    11641 gtattatgtt tttataatat caagatcaat ttcttcaaaa ccaaaaaaag gtaaaatata
    11701 aaaaacaata tctactattt tagtgcttat gaaatacaat caaatatata aagaaaataa
    11761 ttttactaaa ttactttgat aagttagcta ataaatcaaa taccaaaaaa tctttttgta
    11821 tttgatctgg tcaaatcttg gtttgtacga tttaactgtt tcaagtaaat cattgttatt
    11881 ttttattcca tcttctcgca gaaatactaa agaagtcatt atctctaaat catctgtgtt
    11941 atagtcatat attttttcat ataatttctc cgtttttaat tttaagttct gatccaaaaa
    12001 cttgattata gtatctaaat attttcttcc tgaatctgtt aatctatata taaaaatttg
    12061 ttttgaatta tttcctcttt catttttctc atctactata ttgttctgaa ctaaaatatc
    12121 gagttgtctt tttacccatt cagaataagg tccatattga taaaaaatat aatcgttaat
    12181 aatattccaa tatgattgtg tttcgagtat gacaaatcaa gtgatgattg attgtttgag
    12241 agatgagata tttggtaccc ttattatagt gaatattgta tttagtattg tttgtaaacc
    12301 tatgttgaga aattaatcaa caatctattg tataaaataa ttcatttgac aatattagtg
    12361 cattgggaaa agaattttta tattatttaa ctagtaatga gcctataagt taacaaaaca
    12421 ttattttgtt atttcaatat cttttgcatc atcttctatt tctagacatt ccttttctat
    12481 ttggatagtt gaaaaataaa aatcaaattt ttcttaacat ttttgttcaa ctcttaagat
    12541 atcctgtgtt ttagttgtat catctgtttt aatatgtgtc gaaaatatat ttactcatga
    12601 agttatggtc caagcgtatc tgtgaaaatg gttcctataa agaaaatgct gaacagtttg
    12661 tggattcaga aaatttctga aatatgaata acgaaaatgc atatcaagtt aaggaatatt
    12721 taattcatta tatcaggatc agtattaggc ttttctatta ttattagatt tttaatgata
    12781 gtttttatat tttattttag gaattaaatc gtaactttcc tgagtaacat tttctccaat
    12841 catcatggct aatccaattt tgttgctttg ttgctttcta tacttttgca atgatgttat
    12901 aacgctttga gtaagatttt tatcaatagt accctctgta attttaatta atatacttcc
    12961 ataatcctga atagtatttt ttaaagaatc tgaatgtatg ggtttaattg ttgatttaac
    13021 tattaaaatg tcaaaagtaa tatcataaac gctaacatca tgaaaatcat tatgactagc
    13081 tactattgca cattactata caaggtccat tatgttagtt tgcttattat tgtattgctg
    13141 cagctcctgc acttactgca gtactgcagt aagttattat ttgaaggata attacaatag
    13201 agtatgccac tttatgattg tccgcactgt aaacttactt ctaatagaaa atacaatatg
    13261 aaaaagcata tcgagaggaa acatccgggg tcagaaatac cgaataatct gttatcagtc
    13321 agaggtacca atttaaaata tggtagatat gtctgcctcc actgtaaact tacttctaat
    13381 agaagataca atatgaaaaa gcatatcgag aggaaacatc ctgggtcaga aataccgaat
    13441 aatctgttat cagcaagcta caattacaat aataacggat atatccatag aggaccacaa
    13501 aatctcgatg tatattttag agctaaacat aaatcttcag taccattcga cgccacttca
    13561 tatataaata atccatttga tgaggacatt ttaccaattc tcgacactca taaaagtaat
    13621 aaaaattctc attccatatt ttgggaaatg ttgcgatccg ttcaattatt gaatgatctt
    13681 ccaactgagt acatattacc taaaagagat ttttatctgt acaatccaat gttgaatgtg
    13741 aatttttcga atcattataa aaatcagccg cgaccatctg ttgacctgat atcttataac
    13801 aaagagattc tttttaggat ccaaagatgc aataattgta ctacagtgat tcttgttaag
    13861 ttttttggtt ttgataacat taaaccaata attgattgta gatgcgataa catatgtaga
    13921 tcgcaaaaac aaaataaaga aatatcttat ttaacaatca aggaatttct tataaaatca
    13981 attgtctcat ttatcgataa agagatgtat tcaaaaatat acttgaagtg tattaaaatt
    14041 ccttcaaata tctataccaa attaattgtt gaaaaaaaac cattttttct taatgataaa
    14101 gaggttaata ttccttcttg gatgttaaca cctatcatgc ataaagaatt tgttgatcta
    14161 ggagtgatag atgatgacaa ttgggcttat cgtctaacaa acaataatga aaaaaacatt
    14221 gaaataacca agcttgaaat gatggaattc attaattatg gagattccac ttttggacta
    14281 ttcagattcc acaaagacaa tatttcc
//
LOCUS       DASVEV010000091        10590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_805882, whole genome shotgun sequence.
ACCESSION   DASVEV010000091 DASVEV010000000
VERSION     DASVEV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805882"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VF222_04410"
     CDS             <1..669
                     /locus_tag="VF222_04410"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7141700.1"
                     /translation="IRVERTLEQGPPIDLSTLKCQQYLRRHINNKTKLVVLCVDLVGS
                     TKLTLSLTPPELISIINIFSTEMSMIISGYGGYVLKYVGDAVIGIFPADFDAQKACLN
                     SINCAKTMLKVLTQVINPVFYNKLPLIEIKIGIDLGDSMVVLYGKNVDIAHIDIIGSG
                     ISIAAKITALAKPNQILISENIFETVLAVLPDISLKKLDIGEKNWDYNNIKTNKILNV
                     YVLE"
     gene            complement(1000..1539)
                     /locus_tag="VF222_04415"
     CDS             complement(1000..1539)
                     /locus_tag="VF222_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7141701.1"
                     /translation="MILRILNASLLGLLFGFLFQYSFALIEPEELFVFAYQKNTIQDN
                     LSNISVNDSTYLKGYLIIDQYKMFVDIALTDKQKQEGLSIKNSLNENEGMLFFLEEPS
                     KASFWMKNMHFPIDIIWLDANFSIVHIEKELPPCTLAFYCPSYKPLKESLYVLETIDG
                     FANDHHLKVGDKIDFHLLE"
     gene            complement(1595..2011)
                     /locus_tag="VF222_04420"
     CDS             complement(1595..2011)
                     /locus_tag="VF222_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141702.1"
                     /translation="MYSLLFFIAISLLTIIIVQTFYSFLQSGIEIQLFIVSITIELIF
                     VFVIIKVYDELGCKWENVKTIPLLKKKKEYQTTVQNNYYYNNNEDSKTSFCTNCGIPL
                     SYHGNYLNNENNPKFCKNCGYLLPRNILGGYKQKTD"
     gene            2129..2350
                     /locus_tag="VF222_04425"
     CDS             2129..2350
                     /locus_tag="VF222_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141703.1"
                     /translation="MTFYSGVSLNAFSSLPNDLKKNPLKTMTPANAVDAITLSIGRLS
                     KKVTIKEIPAAIVANGNHRFTASSFLCIG"
     gene            complement(2461..2628)
                     /locus_tag="VF222_04430"
     CDS             complement(2461..2628)
                     /locus_tag="VF222_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141704.1"
                     /translation="MIKGSHQNRYIQKAPSSFIEQSECTHCGGNGWIYEFYNDPVSWR
                     SCPQCCEEESG"
     gene            2870..4045
                     /gene="purK"
                     /locus_tag="VF222_04435"
     CDS             2870..4045
                     /gene="purK"
                     /locus_tag="VF222_04435"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0034028 - 5-(carboxyamino)imidazole
                     ribonucleotide synthase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7141705.1"
                     /translation="MSALLDELSIHNQRKIGIIGGGQIGKMIAQEAKRMSIQVIILDP
                     SKYCPASTICDKLIVADFKNEDKIRELAQYSDVITYEIELANSKALKNLELQNYPIFP
                     SPHSLYLIQNKFRQKTFLNENNLPVAKFKKIDSANDLLLVAKEFGYPFMLKACEDSYD
                     GRGNYLIKNKDDIFPAYNSFENREIFAEKFVDFKKEISMMVARNKRGQIESFPIAENI
                     HNNNVLDTTIVPSTISLKIKEAAEIIAKKVVICLNDIGIFGIEMFVSKNDEIFINEIA
                     PRPHNSGHYSIEGCSISQFEQHIRAILNFPLLKPELLRPTVMKNILGPPNFSGNYRIR
                     GLSHLLSIPSTKIHIYGKRVTRPSRKLGHVTCTGPTLDEAIYRCSDAKNSLEIIEDK"
     gene            4054..4566
                     /gene="purE"
                     /locus_tag="VF222_04440"
     CDS             4054..4566
                     /gene="purE"
                     /locus_tag="VF222_04440"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7141706.1"
                     /translation="MSKREVAIIMGSDSDLLVMKDAAEILETFDISYDLTIVSAHRTP
                     ERMVQYALDAAKKGIKVIIAGAGGAAHLPGMIASLTPIPVIGVPIKGLSLDGLDSLLS
                     IVQMPPGVPVATVAINGGNNAAILACQMLGIENKILFDKIEKYKIDIKNKVLSKASKL
                     EQSGWKEYLS"
     gene            complement(4602..4979)
                     /locus_tag="VF222_04445"
     CDS             complement(4602..4979)
                     /locus_tag="VF222_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141707.1"
                     /translation="MTFKKIYLTIAVIPVVLVTIFIVNNITFSNRNDFDLDIDALKEE
                     QSLFIHTRVTITNTGKQPLTNIKVNYGNKTETVSLLEPGHSFPLSPPAGSNLQRIIVT
                     SDQGINITKEYRTPIKMPGMMGS"
     gene            complement(6424..7134)
                     /gene="bluB"
                     /locus_tag="VF222_04450"
     CDS             complement(6424..7134)
                     /gene="bluB"
                     /locus_tag="VF222_04450"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEX7141708.1"
                     /translation="MLFSNSLQTKDRKQITKYEKECFYKVLFSRRDVRSHFIKKNIPL
                     STLLRIINAAHHAPSVGYSQPWNFILVKEEKTRKKIKDSFLKEHERAIKLLEDNKIKQ
                     NKYKSLKLEGILESNINLCVTYDYTRFAPFIIGKTSIPETGIYSVCCAIQNLWLAARA
                     EEIGMGWVSIISNRELKKILNIPKYIKPIAYLCLGYVSEFSHKPDLEKSKWLKRLALN
                     EIIYLEKWGRKVNKKNQI"
     gene            complement(7249..7845)
                     /locus_tag="VF222_04455"
     CDS             complement(7249..7845)
                     /locus_tag="VF222_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7141709.1"
                     /translation="MKLADSKKDQFRRLARDQGYRSRSAFKLKQINESYRILNKGQCI
                     VDIGCAPGGWMQIALSAVGTKGKVIGVDIKKIDPLANAFVIQGSIEEEKIWDSILTIS
                     NSRVDVVLSDLSPNVSGNWELDHARQIDLTRSALNLSRKILKKGGKGVFKVFQGDMLN
                     ELIIELKKGFNKVIITKPNASRQVSSEIYLICIDYKSL"
     gene            7984..9501
                     /locus_tag="VF222_04460"
     CDS             7984..9501
                     /locus_tag="VF222_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7141710.1"
                     /translation="MPINNGREYIESLRNRNLKVYLFGKLVKEPVDHPMIRPSINAVA
                     ETYDIANADPSLASAESSLSGRRVNRFLHIAENTTDLVNQNKMQRKLGQLTGTCFQRC
                     VGMDALNSLYSTTFEIDEKYNTTYHGRLKEFIKKIQEENHVIGGAMTDVKGDRSLSPS
                     QQEDPDLFVHVTKRDENGVYITGAKAHQTGCINSHWILVMPTMRLRDEDKNYAIVGAV
                     PADAEGITYIYGRQSCDTRSMEEGDLDAGNSKYSGQEAMIIFDNTFIPNELIFMDGEF
                     DYAAMLIERFTCYHRRSYVCKTGLGDVLIGAAASISDFNGVANASHIKDKLVEMTHLN
                     ETIFGTGIASSFQSYKTKAGNYQNDDMLANVCKHNVTRFPYEISRLAQDIAGGLVVTM
                     PSEKDFRSPETGPLLEKYLKGRKGVSIEKRVRILRLIENMTLGRNAVGYLTESMHGAG
                     SPQAQRIQIARQMQLEYKMKLARNLANIPESEAEELGKEHSDYFDRIFGLKKEAS"
     gene            9542..10519
                     /locus_tag="VF222_04465"
     CDS             9542..10519
                     /locus_tag="VF222_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7141711.1"
                     /translation="MKNTVLDIVDLNKYFRLRTGIIDVISFKTSKKIKVIDNINLKLC
                     KGEILVVAGESGSGKTTLAKLIMRSIDPDSGKIIFDNRDITKLKGKELLKIRSKIQMI
                     FQDPYSSLDPRMKIFDIVKEPLEVHKKKISKNEIKECIDSTLKKVNLDPEEVSPKYPH
                     MLSGGQRQRVSFARALVLKPVLIIADEPVSMLDVSIRFQILELMKNLRDKENVSFLYI
                     THDLSTSQYVGDNIIILYKGQIIEQGNIHEVLRHPLHPYTKALISAIPTFNDYQEIKI
                     KTELTSSGKCGCKFLSKCLYSSEECSKTEPELIEANHSHFIRCYNYESI"
BASE COUNT         3699 a         1608 c         1653 g         3630 t
ORIGIN      
        1 attagagttg aaaggacctt agaacaagga cctccaattg atctatcaac attaaaatgt
       61 cagcaatatt taaggcgaca tatcaacaat aaaactaaac ttgttgttct ttgtgtagat
      121 ctggtcggat ctacaaaatt aactttgtcc ctgacaccac cggaattaat ttcaattatt
      181 aatattttct ctactgagat gagtatgata atttctggat atggaggtta tgttctaaaa
      241 tatgtaggag atgcagttat aggtatattt cctgctgatt ttgacgcaca gaaagcatgt
      301 ttaaattcca tcaattgtgc aaaaactatg ctaaaagttc ttactcaggt tattaaccct
      361 gttttttata ataagctacc tcttattgag attaagatag gtattgactt gggtgactcc
      421 atggtagtat tgtatggaaa aaatgtcgat atagctcata ttgatattat tggttctggt
      481 atcagtattg cagccaaaat aaccgcctta gccaagccaa accaaattct tatcagtgaa
      541 aatatttttg agactgtctt agcagttcta ccagatataa gtttaaaaaa attagatatt
      601 ggtgagaaaa actgggatta taacaatatc aaaacaaata aaattttaaa tgtatatgta
      661 ttagaataac tgatattatt ccattgctta tatttgtcca atgaatatta tagaaaatac
      721 atttaacaag tctcgcgacc ttcaatattt taataaaaga tttgtagatt aaaatcttga
      781 agtacaaata atttgatgat tattattatt tattatgatt atttaaggaa aaaatattat
      841 tactaaattc tttctaaatc ttccatattt tttttaatgt tacgggaaac agaagctcca
      901 aacattagag aaaatgtaga ttatggagaa aaataaaatt cagaaagatt aataaacaat
      961 attaatagat agttttgaaa taagtaggaa atgtccaatt tattccaata agtgaaaatc
     1021 aattttatct cctaccttaa gatgatgatc gtttgcaaac ccatctattg tttctaaaac
     1081 atataaagat tctttaagtg gtttgtaact tggacaataa aatgctaagg tacatggtgg
     1141 gagttctttt tctatatgta caatagaaaa gtttgcgtct aaccaaatga tatcgatagg
     1201 aaaatgcata tttttcatcc agaatgatgc tttactaggc tcttccaaaa agaacaacat
     1261 tccctcgttt tcattcaaag aatttttaat ggataatcct tcttgttttt gtttatcagt
     1321 aagagctata tctacaaaca ttttgtactg gtcgataatt aaatatcctt ttaaatatgt
     1381 tgaatcattg acagaaatat ttgataagtt gtcttgtatg gtgtttttct gataagcaaa
     1441 tacaaataac tcttctggtt ctattagtgc aaaggaatat tgaaagagga atccaaatag
     1501 taaacctaac aatgatgcat ttagaattct taggatcaac atttatttac taaatataaa
     1561 aaaataagta atttaaccat tatctattca aatattagtc agttttttgt ttatatcccc
     1621 caagaatatt tctaggaagt aaatagccgc aatttttaca aaactttgga ttattttcat
     1681 tgttcaaata atttccgtga tatgataatg gtattccaca attagtgcaa aaactagttt
     1741 ttgagtcctc attgttattg tagtaatagt tgttctggac tgttgtttgg tattctttct
     1801 ttttttttaa aagaggtata gtcttaacat tttcccattt acaacctaat tcgtcatata
     1861 ctttgataat aacaaaaaca aatattaatt ctatagtgat agaaacgata aatagctgaa
     1921 tttctatccc actttgcaga aacgaataga aagtctgaac tattataatc gtaagtaaac
     1981 ttatagctat gaaaaataat agtgagtaca attggcttga gattttaacc atttaatata
     2041 attaacaacg caaagaaact aaaaataatc ctatgatcaa ttttttaaat catttgataa
     2101 aaacttgttt tcaacaattt attcaaaggt gacattttac tctggtgttt ctttaaatgc
     2161 attttcatct ttgccaaatg acctaaaaaa aaatccgcta aaaacaatga ctccagccaa
     2221 tgcagtagat gcaattaccc tctcaattgg aagattgagt aaaaaagtaa caataaagga
     2281 gataccagcc gcaattgtag ctaatggaaa ccatcgcttc actgcctctt cttttctttg
     2341 catagggtga agtataaggt ccttcttgtt taatttatct tttcttaaaa gaattaagaa
     2401 aaataatata atttaatcct catcattctg gggattaaac cgaatttaaa aaaatactct
     2461 ttatccactt tcttcttcgc aacattgggg acatgatcgc caagacacag ggtcattata
     2521 gaactcatag atccatccat ttcccccaca gtgagtacat tcagattgtt cgataaacga
     2581 tgatggtgcc ttttgaatgt atcgattctg atgggaacct tttatcatac aatattctag
     2641 aaggttcttc taattaaaca ttgtgaaaaa ttttagttaa tataatagta gaacatttgt
     2701 agttaaatca acctattctt ttgaagaata ttacatcaat ataaataaat aaatattttt
     2761 ctttatcatt aataatgtgc ttaagctttt ttaaatttaa tagtaattgt agtttacatt
     2821 ctttgttgat aagtttttaa cagtctttta caattaagat tactgcttta tgtctgcttt
     2881 actagatgaa ctctccatac ataatcagag gaaaataggt attattgggg ggggacaaat
     2941 aggaaaaatg atagcacagg aagctaagag aatgtcaatt caagtaatta tattggaccc
     3001 tagtaaatat tgtcctgctt caactatatg cgataaacta attgtagcag attttaaaaa
     3061 tgaggacaaa attagggagt tggcacaata ttcagacgta attacttatg aaatagaatt
     3121 agctaactca aaagccttaa aaaatcttga attacaaaac tatccaatct ttccatcccc
     3181 tcactctctc tacctaattc aaaataaatt tagacaaaaa acattcctaa atgaaaataa
     3241 tttacctgtc gcaaaattta aaaaaataga ttcagctaat gacttgttat tagtagcgaa
     3301 ggaatttgga tacccattta tgctaaaggc gtgtgaagac tcctacgatg gaagaggcaa
     3361 ttatcttatt aaaaacaaag atgacatttt tcccgcttac aattcttttg agaatcgtga
     3421 gattttcgcc gaaaaatttg tagattttaa aaaagaaata tcaatgatgg ttgcaaggaa
     3481 taaaagagga caaattgaat cttttcctat agcagaaaat attcacaaca ataatgtgct
     3541 agatacgaca atagttcctt caaccatttc tttaaaaatt aaagaagctg ctgagattat
     3601 tgcaaaaaaa gtagttattt gtttgaatga tataggtata tttggaatag aaatgttcgt
     3661 ttccaaaaac gatgaaatat ttataaacga aattgctcct cgtccacata attctggtca
     3721 ttatagcatt gagggttgtt caatttctca gtttgaacaa catattaggg ccatattaaa
     3781 ttttccttta ttgaagcccg aattgttgag acccaccgtt atgaaaaata ttttaggtcc
     3841 acccaacttt tcaggaaact atagaattcg agggttaagt catttattat ctattccctc
     3901 tactaaaatt cacatttatg gaaaacgtgt aactcgtcca agtaggaaac taggacatgt
     3961 tacttgtact ggtccaactt tagatgaggc tatttataga tgctcagatg caaaaaattc
     4021 attagagata attgaagata aataacatac gaattgtcga aacgtgaagt tgccattatt
     4081 atgggaagtg actctgatct cctggttatg aaagatgctg ctgaaatatt agaaactttt
     4141 gatatttcat acgacttaac tattgtttca gcacatagga ctccagaacg aatggttcaa
     4201 tatgctcttg acgcagcaaa aaaaggaata aaggttatta tcgcgggtgc aggaggtgct
     4261 gcccatctac caggcatgat tgcctcatta acccctattc ctgtgattgg tgttccgata
     4321 aagggtttaa gtttagatgg attggattct ttgttatcta ttgtacaaat gccccctggg
     4381 gttcctgttg ctactgtagc aataaacgga ggaaacaacg cagctatctt ggcttgccag
     4441 atgttaggaa tcgaaaataa aattttattt gacaaaattg aaaaatataa aatagatatt
     4501 aaaaacaaag tactatctaa agcaagtaaa ctagaacaaa gtggatggaa ggaatacttg
     4561 tcataataaa aattgtttta ataaaaaaat tttattccct tttacgaacc catcattccg
     4621 ggcattttaa ttggagttct atattctttt gtaatattaa tgccttggtc cgaagtaaca
     4681 attattcttt gaagattgct acctgctggt gggctaagtg gaaaagagtg tcctggttct
     4741 agaagtgata cggtttcagt tttgttccca taattaacct tgatatttgt aagtggttgt
     4801 tttccagtat tagttattgt aactctagta tgaataaata atgattgttc ttcttttaaa
     4861 gcatcaatgt ctaaatcaaa atcatttcta tttgaaaagg taatgttatt tactataaaa
     4921 atagtaacta gtactactgg tataacagct atagtaagat aaattttttt aaacgtcatt
     4981 aataataatt atcaccatag aaatattatt tttcgcattt agtgaaaaga tataaaaagt
     5041 attttcaatg attgcatatg tgaggttttg aacgatatat gattatgggt caagtcaaag
     5101 agaactttag taataacgct tgtacgatga acaatactaa acctaccact agtaaatgta
     5161 tcgttttact tgttataaga tccaaatcct aagaatcact atattttgct atatcgcctg
     5221 aaaatctttt ttataggtgt ccaaccatgc cattgagtta aaattcttgg atttttatac
     5281 caatctgggg gctatactat tagtagaacc ttattgaata tttgctttcc ttccttctat
     5341 gtcattaact ttttatctat ggagaatcct cgacccacaa tacaaatgag tataagctac
     5401 agcttttgta tccccttccc atttgaataa atgttggtga aaaatggata acttagcaaa
     5461 gattagaaca actcgaagtt tttagtggat ttccagatgt ttggtataat gagggaatta
     5521 ttcaatcgaa ctatggtcta gtatttaatt atatcaactt cattaatcta cataaactgt
     5581 tccctgtaag tttattcatt ttcaccataa tatgatggtc atggtgtttc gaatacctac
     5641 cgggtttaag taacgtctaa atgtagaatt atggctgaaa tgttttattt gtgaaatgaa
     5701 tgcaagctag aagatacacc cattgttata gagggagtta actaactaat gcaaatgaga
     5761 attgatttgc cacttattac tgaatagtgt ggtattagta aatatcctcc agtttccttc
     5821 attctgcagc agcaaggata taaatggtac attctgtaca tcgattaagt cctcaggttt
     5881 tctattacaa gaatcttaat ttctctcctg cctcctctag aatccttgtc atggttgtat
     5941 gatagtgttt gttattgttg ttatttacta ttaataaata ataattgtaa aaacaaggaa
     6001 ggaagaaaat ccttttttag taacatttat gggataaata ctattcatat tttgaattat
     6061 tttaccagga atcaaaaatt ggtggttaat acatgaatat gcttttagtt taaagtacat
     6121 ttcttaaaaa atgtaacaaa aagaaaatat ttttatgtaa atttgtattc caaaaaaaaa
     6181 gtcatttcat gcataaacaa tagtaattaa tatcaataaa taacaagtga tatcaactgg
     6241 tagtagcaaa tatcaagaaa taaactaaaa aaaataacat aatgtatgta gtacgatcat
     6301 ttatatatca attatttact gatgaatatg caacatggat tattattaac caatcgagaa
     6361 aaatggaatc tagtaaaaaa ataattaatt cttatttaaa atagtggggt ctcttattat
     6421 tctttaaatt tgattttttt tattaacctt tcttccccat ttctctagat atataatttc
     6481 gttaagagcc aatctcttca accactttga tttttccaag tcaggcttgt gtgaaaattc
     6541 tgaaacatat ccaagacaga gataagctat gggttttata tatttaggaa tatttaatat
     6601 cttttttaat tctctattcg agatgatact cacccaaccc atccctattt cctctgctct
     6661 tgcagcaagc cataaattct gaattgcaca acatacacta taaattcctg tttcaggaat
     6721 tgaagtcttt cctattataa atggtgcaaa tctagtataa tcataagtaa cgcataagtt
     6781 gatgtttgat tccaaaatcc cctctagctt cagagatttg tatttatttt gttttatctt
     6841 gttatcttct agtaacttaa ttgccctctc atgttctttt aaaaaagaat ccttaatttt
     6901 tttccttgtt ttttcttctt ttaccagtat gaagttccaa ggttgtgaat atccaaccga
     6961 tggtgcatga tgtgcagcat taataattct caataaggtt gataatggga tgtttttttt
     7021 gatgaaatga gaccgaacat cccttcttga gaatagaact ttgtaaaaac attccttttc
     7081 atattttgta atttgttttc tatcctttgt ttgtaaacta tttgagaata acaatatcca
     7141 ttttttatca atgcattata agtaggttca aagttaatgt gaaaatattt ttttaagaaa
     7201 gtcaaccaaa ttctataatt tctaaagatg aaaataatac aacacaaatt acagtgattt
     7261 ataatcaata caaattaggt atatctcact actgacttgt ctacttgcat ttggttttgt
     7321 tattataact ttattaaacc cttttttcag ctcaataatt agttcattaa gcatatcacc
     7381 ttgaaaaact ttaaaaacac ctttgcctcc cttctttaaa attttacgac ttaaattcag
     7441 agcacttcta gttaaatcta tttgtcttgc atggtctaat tcccaatttc cacttacatt
     7501 gggtgacaaa tctgataaca caacatcaac acgtgaattt gaaatcgtca gaatagaatc
     7561 ccatatcttt tcttcttcaa tactaccttg aataacaaag gcattagcta atgggtcaat
     7621 tttttttata tctactccta ttaccttacc ttttgttcct accgcagata atgctatttg
     7681 catccagcca cccggagcac aaccaatatc tactatgcat tgacctttat tcaatattct
     7741 ataagattca ttgatctgtt tgagcttgaa ggcggatcta cttctgtatc cttgatctct
     7801 ggctaatcgt ctgaattggt cttttttaga gtctgctaac ttcatatcta cattaaacta
     7861 aattaaaaat aaacttctta taatattacc tatatctcta ccgaaaacac ttaaattttt
     7921 ctaacgcact tttggaaatg ccgataatat ataaataata tccaaacgtt atatatataa
     7981 tatatgccta tcaacaatgg tcgagagtat attgaaagtt taaggaatag gaaccttaag
     8041 gtgtatcttt ttggaaaatt agtaaaagaa cccgtggatc atcctatgat tagaccatca
     8101 ataaatgctg ttgcagaaac ttacgatata gctaatgcag atccatcgct tgcatctgct
     8161 gaatcctctc tttctggtcg acgagtgaac aggttcttac atattgccga aaatactaca
     8221 gatttagtca accaaaataa aatgcagcga aagcttggtc agctgactgg aacttgtttt
     8281 caacgatgtg taggcatgga tgctttaaat tctttatatt ccactacatt tgaaattgat
     8341 gaaaaataca atactacata tcatggaaga ttaaaggaat tcataaaaaa aatccaagaa
     8401 gaaaatcatg tcataggagg agctatgaca gatgttaaag gggacagaag tctgtctcct
     8461 tcccaacaag aagatccaga tttatttgtt catgttacga aacgtgacga aaatggtgtt
     8521 tatattaccg gggcaaaagc tcatcaaact ggttgtatca attctcattg gattttagta
     8581 atgccaacta tgaggttgcg tgatgaagat aaaaattatg caatagttgg cgctgtgcca
     8641 gcagatgctg aaggaataac gtatatctat ggaagacagt cctgcgatac aagaagtatg
     8701 gaggagggtg atctagatgc aggtaattct aaatattccg gccaggaagc aatgataatt
     8761 tttgataata cattcattcc taacgaactt atctttatgg atggagagtt tgattatgct
     8821 gccatgctta tagagcggtt tacttgttat catcggagaa gttatgtatg taaaacagga
     8881 ttaggtgatg tactcattgg tgcagcagca tctatttctg attttaatgg tgtagccaat
     8941 gcctcacata ttaaggataa attggttgaa atgactcatc taaatgaaac aatatttgga
     9001 acaggtattg cctcctcttt tcaatcatat aaaactaagg ctggtaatta tcaaaatgac
     9061 gatatgcttg ccaatgtttg taaacataac gtaactcgct ttccttatga aataagcaga
     9121 ttagcgcaag acatcgctgg aggattagta gttaccatgc cttctgaaaa agattttcgc
     9181 agccctgaga ccgggccact tttagagaaa tatctgaaag gtaggaaagg agtttctatt
     9241 gagaaaaggg tacgaatctt acggctcatt gaaaatatga ctttaggaag aaatgcggtt
     9301 ggttatctga cagaatcaat gcatggggca ggctctcctc aagcacagag aatccaaatt
     9361 gctcgtcaaa tgcaattaga atataaaatg aaacttgcaa gaaacttggc aaatattcct
     9421 gaaagcgagg cagaagagct aggcaaagaa cactctgact attttgatag gatatttggg
     9481 ttaaagaaag aagcatctta accaaattca aattttttgt ttcacaagtg tacaaattaa
     9541 attgaaaaac acagtgctag atatagtaga tttaaataaa tattttagat tacgtactgg
     9601 gattattgat gtaattagtt tcaaaacctc taagaagata aaagttatag ataatatcaa
     9661 tttaaaactc tgtaagggcg aaatacttgt agttgcagga gaatctggtt caggaaaaac
     9721 aactctagcg aaacttataa tgcgatcaat tgatcctgat tcgggtaaaa taatctttga
     9781 taatagagat attactaaac tcaaaggaaa agagttacta aaaataagat ctaaaattca
     9841 aatgatcttt caagatcctt attcttcttt ggatccacga atgaagatat ttgatattgt
     9901 taaagaacct ctagaggtac acaaaaaaaa aatttcaaaa aatgaaataa aagaatgtat
     9961 tgattctact ttaaaaaagg taaatttaga tcctgaggag gtatctccta aatatcctca
    10021 tatgttatcg ggaggccaaa gacaaagagt tagttttgca agagctcttg ttcttaaacc
    10081 tgttttaatt atagcagacg agcctgtctc aatgttggac gtttctatac gttttcagat
    10141 acttgaatta atgaaaaact taagagataa agaaaatgtt tcctttttat atattacaca
    10201 tgatctctct acttcacaat acgtcggaga taatataatc atcttatata aaggacaaat
    10261 aattgagcaa ggcaatattc atgaagtctt acgacaccct cttcatcctt acactaaagc
    10321 tttaatatct gcaattccaa catttaatga ttaccaagaa ataaagataa aaactgagct
    10381 cacatcatct ggtaaatgtg gctgtaaatt tttatctaaa tgtctatatt caagtgagga
    10441 atgttctaaa actgagccag aattaattga agcaaatcat tcacatttta ttcggtgtta
    10501 taattatgaa tccatttgac ctacctttat ttctaatcgt ttaagttaaa gggatttcat
    10561 ctaattcctt tttatgatat ttatacttga
//
LOCUS       DASVEV010000092         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1389769, whole genome shotgun sequence.
ACCESSION   DASVEV010000092 DASVEV010000000
VERSION     DASVEV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389769"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF222_04470"
     CDS             complement(<1..278)
                     /locus_tag="VF222_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141712.1"
                     /translation="MKLDAYTKSTLVFLIVLSLSISIIHFENTKAQTIGSTDPKELLK
                     EFKTGKDAIQNDIQTNFNQLINEVKSQLNFSSVDSHLSLASQNFINGR"
     gene            472..1062
                     /locus_tag="VF222_04475"
     CDS             472..1062
                     /locus_tag="VF222_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7141713.1"
                     /translation="MKKSPKWVRAAQIILGALAILLAIVALTNSGLTILLILIAIILV
                     ITGFEKTITGLFIAHNLRFFTIGLGILTIILAGLALTYPIAAVRVLLLILGFSLMVDG
                     FSRIADGLTNKSDKRLIRGFTIAVGILAIIISVGINIIPFWGKLFISKLIAIDLIIIG
                     VQIIISGTVNVEAGKKKVKEKVKKIFQRQEQSEEKK"
     gene            complement(1444..1680)
                     /locus_tag="VF222_04480"
     CDS             complement(1444..1680)
                     /locus_tag="VF222_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141714.1"
                     /translation="MKKSVIAIQSCVITTIVYALLAPIFFEMDNAFGEGEYFVVVYDD
                     YNKDQMMSTQQLVETKECKSPCPSTAEMCIAMCA"
BASE COUNT          741 a          251 c          301 g          726 t
ORIGIN      
        1 attctcccat ttatgaaatt ttgagaggct aatgaaagat gtgaatcaac agaactaaaa
       61 tttagttgtg atttaacttc attaattaat tgattaaaat ttgtctgaat gtcattttga
      121 atagcatcct ttcctgtttt gaattctttt aataattcct tcggatcagt tgaaccaata
      181 gtttgtgctt ttgtattttc gaaatgtatt atagaaatgg aaagactgag aactattaaa
      241 aatactaaag tagattttgt atatgcatct aacttcataa cgttaaaaat tcaatacttg
      301 tatatatttt cgttggttaa ataattgctt atgtatgaat aaatggtata tttaatgatg
      361 agatttgtat tatataatgt aatcctgctt catcggttaa ttcttaagag aatattttaa
      421 ttattggaaa tatacaactc tactaaataa aggtctgtaa taacggtaac tatgaaaaaa
      481 tctccaaaat gggtgagagc agctcaaatc atacttggtg ctttagctat cttacttgca
      541 attgttgcac taacaaattc aggtctaaca attttgctca ttttaatagc tataattttg
      601 gttataacag gatttgaaaa aactattact ggattgttta ttgctcataa cttacgattc
      661 ttcactatag gattaggaat attgactatt attctagcag gattggcatt gacatatcct
      721 atagcagctg ttagagtact tttattaatt ttgggatttt cattgatggt agatgggttt
      781 tcacgaatag ctgatggatt gaccaataaa tctgacaaga gactaattag aggatttacc
      841 atagcagtag gaatattagc cataataatt tctgttggta ttaatatcat tcctttctgg
      901 ggaaaattat ttattagtaa attaatagcg atagatttga taattatagg agtacagata
      961 attatatctg gaactgttaa tgtagaagcc ggaaaaaaga aggtaaagga aaaggtaaag
     1021 aaaatctttc aacggcaaga acagagtgaa gaaaagaaat agtacctaaa aggaaatttc
     1081 ttgtaaatta tctttactag tagggatgct ataaaatctg gtctttttat atatgaacca
     1141 ggttatagat aatgcccgta gttttctcga attcatatac ttccctttat tatctatcct
     1201 ctatctctta tctcagtata gatgtgaaag aatgcactag tatccttgaa ggttttgtaa
     1261 aaagcaatga gcatattatt caaggataaa taaagtaggc cgtctcttcc ataattcctt
     1321 taagtttgaa tattgagatt gtaaataaaa ttaggactat taagacacag gaatctatta
     1381 ctaatttact agttcagaaa agataaaaat agaattaaca aaaaggaaat aaaaataggt
     1441 tttttaagca cacattgcaa tacacatttc tgcagtagat gggcaaggag atttacattc
     1501 tttagtttct actaattgtt gagtagacat catctgatct ttattataat catcataaac
     1561 aacaacaaag tattctcctt ctccaaaagc attatccatt tcaaaaaata taggggctag
     1621 aagtgcatat acaattgtag ttatcacaca tgattgaatt gcaataactg attttttcat
     1681 atatttattt ttcctattat atatacggca tatttgaata agagtatttt aatacatgaa
     1741 tagacaaata tttctataaa tgttttcgaa aaaagttatt tttggctata aacaaaaata
     1801 aaaatatatc caaatagaat tagctcatat agatataaaa ttataattgt attcctctag
     1861 cggtatttta ttaaggttag taacaaaaaa ttcaactaat catttttcct aattgatttt
     1921 aaaataaaaa ttagcaattg gaaatcataa taaacattaa ctaatctaat ttagtaatac
     1981 tatgttagca agtgtaccta agcacttcaa ttatatttt
//
LOCUS       DASVEV010000093        20009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1002621, whole genome shotgun sequence.
ACCESSION   DASVEV010000093 DASVEV010000000
VERSION     DASVEV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002621"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..444)
                     /locus_tag="VF222_04485"
     CDS             complement(70..444)
                     /locus_tag="VF222_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141715.1"
                     /translation="MQKWTKQTTTIKIKNQTKVYQLAVSGNNVYVTWYDFTPGNEDIF
                     FAVSSDNSQTFSTPINLSNNTGNSFTPQIAAIGNNVYVTWGDGTPGNNDIFVITNTQP
                     FGTPVNISNNPGSSANPQIATS"
     gene            896..1099
                     /locus_tag="VF222_04490"
     CDS             896..1099
                     /locus_tag="VF222_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141716.1"
                     /translation="MNNAGIKITTLIIMTVLTGLGMLLAYPTITISAQENMTNPDQNM
                     TNPDQNMTALGSGTISGAGRGAG"
     gene            1138..2544
                     /locus_tag="VF222_04495"
     CDS             1138..2544
                     /locus_tag="VF222_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141717.1"
                     /translation="MNSPLNFFTLHIKKLLNFGTKNIHIVIYVLIAVLSLDTLLNQTG
                     DLIGQPLKTFGGIVLFITLSIITMIGQIFVLQFVAKKSEEIRKKNGKIRLMHRFVSIS
                     QYTIITIFVFLILEIIINQNYLPISSMLVTLISYMLSTILMAIFTYVFLSWYKTNRNS
                     VLVLVYGLSFATAAIASFAILLVFTYQFSEKLFISILPTSPGTFLSTEEGSIWKIFAK
                     TYQYNDMASFFLKWGGTALILYHYSYKIGRAKYWFLLSLPLLYFSTLMVYHLHIYEPH
                     EEFEQLIFYGVVALNSTFGGILFYIAFKLTVKNFKGNELFASYLIMAGYGFMLFFSGT
                     QSVLTNMAYPPFGFSTVSSYGLGSYLILVGLSLSAISISKDEQLKEMIKNSTMAESKF
                     LHSIGMSESERENKLLKDIVIKAKQQKENLEKDTGIELSISEEDIKEFVREVEVNQND
                     KDKDKDNENKEDEEKGNQ"
     gene            complement(2692..3039)
                     /locus_tag="VF222_04500"
     CDS             complement(2692..3039)
                     /locus_tag="VF222_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141718.1"
                     /translation="MSDKSFIDKAKDTIGDVTEKASDTMGKVTKLQKQSKLKAGYIER
                     DEFKNSNFQKGFFCYNCIYWINMTGGKCMMVNDKGPDLFGKVSDVIAPHGCCSIYTPN
                     FDEINKQKVVIVD"
     gene            complement(3048..3326)
                     /locus_tag="VF222_04505"
     CDS             complement(3048..3326)
                     /locus_tag="VF222_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141719.1"
                     /translation="MSSNFSSTQKIPQQATEHNKLTKVTSGYMEIEEFKDVDTHKGYF
                     CYNCDYFIKPEHCMIVTDEGQDLDGNISGKILPYAICSLWTPNEKEIK"
     gene            complement(4217..4618)
                     /locus_tag="VF222_04510"
     CDS             complement(4217..4618)
                     /locus_tag="VF222_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141720.1"
                     /translation="MIIDDDKDITNLFAIFLEYNGYIIDAYTNPVEAFNNFKKNSHDL
                     IILDLKMPRIDGMTLYHKIKEIDNNVIICFTTADINYIQQLQKSIIDIEKIVLYKPVL
                     LKDLKNKIEWLLSSQEVHNSNRPTTTMMMIL"
     gene            complement(4877..5131)
                     /locus_tag="VF222_04515"
     CDS             complement(4877..5131)
                     /locus_tag="VF222_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141721.1"
                     /translation="MDTILKSIIKRQIPFLIGGTITGSIMAYYYGFLFTIIVNSIMWY
                     VISYLVSKYYYKSKGINDQKYLIQYGLSKIKSRNKTVKTT"
     gene            5834..6043
                     /locus_tag="VF222_04520"
     CDS             5834..6043
                     /locus_tag="VF222_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141722.1"
                     /translation="MSHITDANKWREESLKGLLCTKFQCYNKPRNQCSECSIYYCNEH
                     VTSHIHTDTFQDLQREQEQDIDKTR"
     gene            complement(6296..6418)
                     /locus_tag="VF222_04525"
     CDS             complement(6296..6418)
                     /locus_tag="VF222_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141723.1"
                     /translation="MSDNKINNCLNCGSSLNNSYYSSNFCSGQCKDANIRHIAG"
     gene            6875..7294
                     /locus_tag="VF222_04530"
     CDS             6875..7294
                     /locus_tag="VF222_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141724.1"
                     /translation="MPNLDPWINQNEEFMAATNTINKFENHLLSIKDEHQKRIRPNLH
                     PTYNERHQKIIEMIDWSLEKYKAAVATNIKKKNQQNKEEVIIDNIIEELDKKRDIARN
                     KMKKALLKDEVSIYSLEENTLDYVLFIICELTGNKLY"
     gene            complement(7495..7752)
                     /locus_tag="VF222_04535"
     CDS             complement(7495..7752)
                     /locus_tag="VF222_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141725.1"
                     /translation="MIYNKLFLILSTVAILPIVLTLVTITIPTFAQSEGTNETMQNAG
                     ESANKTGESMQQNASGMGSKITEGAKNMAENIGEGLKDLTK"
     gene            complement(7755..7943)
                     /locus_tag="VF222_04540"
     CDS             complement(7755..7943)
                     /locus_tag="VF222_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141726.1"
                     /translation="MICIITITMENDKDIKIETEEEVGLDDKIKAGARALGKKVEDPD
                     RDTGEEYEAEKGKERVVD"
     gene            complement(8381..8644)
                     /locus_tag="VF222_04545"
     CDS             complement(8381..8644)
                     /locus_tag="VF222_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7141727.1"
                     /translation="MGNRSRTEIESNIFDAANGGTTKTKIMYIAFLSYGQLKEYLSIL
                     IENNLIEYLDGTHQFKTTEKGLKLLKMHNEMAQLLQATTIKKE"
     gene            9046..9678
                     /locus_tag="VF222_04550"
     CDS             9046..9678
                     /locus_tag="VF222_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141728.1"
                     /translation="MSTKEDKINETNLNRAKFNTDNTNTTTNFIERTNRDYNNTLNQQ
                     QDAINKTLDNTLNNVKRTTDEATREIPRYTQRIAEYQEQTIQTVKDIASDFIEAEKQV
                     IGSFQSQVDRNSGNTNGLWNWFNPQRIAENHAKMVNNFTSYLLNTSNLINNALASNMR
                     VYNTALEQTRDNLKACAKTNTNFIQSVNRQNRDGNTSNNNNNINERPTRY"
     gene            9825..9974
                     /locus_tag="VF222_04555"
     CDS             9825..9974
                     /locus_tag="VF222_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141729.1"
                     /translation="MSIEYRKRLLTITYSEPEKTTGPSGVIPIAVLKDESNVEISVLV
                     FSFYY"
     gene            complement(10208..10933)
                     /locus_tag="VF222_04560"
     CDS             complement(10208..10933)
                     /locus_tag="VF222_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141730.1"
                     /translation="MSILVTLFSIATINNIFSNVMAQGYDTYGDSYSTYPTKDKKIAC
                     QTGQFEGFFVESVEFCKLTIAQGPPGQNATQVTINNIRDILTNQTLQCVLNTSVDPAS
                     IDCALPPKSTTPTTLSVSLTIKCTPDDSNPRSVQACNRITTEILPNIYNIDITGNHPN
                     PNRFNGSPVPVIVTLGPDVFLVFVTVSTQVIDTIIDNIQSDLNVNIFGPTEHFIGHCP
                     SGEIFAGESLTCNIEERLTVASN"
     gene            12344..13006
                     /locus_tag="VF222_04565"
     CDS             12344..13006
                     /locus_tag="VF222_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX7141731.1"
                     /translation="MNYNQRNNSFTNHHDFQHYVLLYNDTKSRDDYLINYINQGLNKN
                     ELCIYASVGLRDKDTLKFVKRIVDYEKNISENNLILVDLAPFYIAALTYDFRVFKETA
                     NEFSKKIKNRENKNIRFSSDGAGLLYDNKHFGLCESIESWWQHKRPFEGIVLCPYQKS
                     NLEKYPFNLHKDNAIGKQHDNFIDAESILNKDRIANKKEINSSKEINNKEDRKEYGGS
                     NR"
     gene            13006..14052
                     /locus_tag="VF222_04570"
     CDS             13006..14052
                     /locus_tag="VF222_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141732.1"
                     /translation="MPPPSSSQQSTINSIGNAHIMLLYESDENRNNILIDYINEGLKN
                     GCFCFYASVDIDNSKNMSLIDSLSSRIINYEENIQNENLQFINFRPYYESALKGDLTL
                     FEELKRDLENILYKRISEGKKDKILFFADAASHLSETMNFSQSIDLEKWWQDVSLEWR
                     RNNKDITIVCPHPNYIFKDVSLDYTKFKITDSHDMTIDIQDKHSLQYVYKLIKNNRQI
                     IRILIAEPEPDIRYLYREYFNGLELEVEIVENGSKCIEYLLDSKDKDKGEEEGFDMVI
                     LDSHLPDTNGVEVLKTIREKMPYQRIVFTTTHPLSKINPVVDPFGIEEDDILVKPFSF
                     TKLLSTIKPRTTTK"
     gene            complement(14111..14527)
                     /locus_tag="VF222_04575"
     CDS             complement(14111..14527)
                     /locus_tag="VF222_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7141733.1"
                     /translation="MELSKILVAVDGSKYNDKAFEYASSIIQNHKQQLLLVHIIENVG
                     NIGYSNFNQLKQDSQQILQKYKTKAESNGLKSSLKIIEEQGNSPAEKILDIAETEKVD
                     AIIVGTKGQKTSQQFLLGSTSYKVAHYSKCTVIIVK"
     gene            complement(14728..14925)
                     /gene="pgk"
                     /locus_tag="VF222_04580"
     CDS             complement(14728..14925)
                     /gene="pgk"
                     /locus_tag="VF222_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7141734.1"
                     /translation="MGIFEIELFRNGTNNIAKSMALAFWRGSKTVIGGGDTLSALKKA
                     EVSELEVTHVSTGGRALNKIK"
     gene            complement(15133..15822)
                     /locus_tag="VF222_04585"
     CDS             complement(15133..15822)
                     /locus_tag="VF222_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX7141735.1"
                     /translation="MRWSIEDFVYGGTDGVVTTFAIVTGAFGASLPVSVILILGFANL
                     FADGFSMSIGNYLSARTHKEIIEKERRKEEWSIDNLTEEEREEIREIYRNKGFKDELL
                     EDLVKVITSKRKVWVDTMMREEIGSIDDKKNPRDTAITTFVAFNVLGLIPLIPFIIIY
                     AIGILTISASTAFIYSIVFTGISFFVIGIVKGKIVTKSVLQSGLHTLAIGGVAAIVAY
                     TVGYILGIYIE"
     gene            complement(16142..16972)
                     /locus_tag="VF222_04590"
     CDS             complement(16142..16972)
                     /locus_tag="VF222_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141736.1"
                     /translation="MANNQILLILPILLALTIHVATTEAFAQPGSIPTLQHIDATYAV
                     SIVPGAAQKENIHHYYPPQIAVPTGTTVGWFNNDFGQPHTVTSGQPKATDEGSIFNSG
                     IMPATANSLFQFTFTQPGEFLYHCIIHPWRVGAVTASDAFFTGEGFKIDIGSGAVWDI
                     TTHPRVLLDIEPQTIPLDRNTPITYNVTISEDENNNDKILFSKLFTTAGQSLPLELIS
                     AEGNETISYGPDFSSTGAYHVQSDFKKGSTYPISVEIISINSKPVESPIKVLFDFRTA
                     "
     gene            17357..17779
                     /locus_tag="VF222_04595"
     CDS             17357..17779
                     /locus_tag="VF222_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7141737.1"
                     /translation="MSKKIETVEEFASVQEVAIKMKDKNISSLIVVDSFGKSKGLVTE
                     RDLVRKICVKDVCPSKVIIKEIVSLPLVTINPNESPSTAIDIMLQHNIRHLVVVDDSM
                     EINKPIGIVTPRDLTRYQEFTRDDTNNDNLEKILEYFI"
     gene            18315..19238
                     /locus_tag="VF222_04600"
     CDS             18315..19238
                     /locus_tag="VF222_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141738.1"
                     /translation="MLVLKYILLHKLNSITNLNFLKNSIINLNRYINSNVLIQDVDSL
                     LQKIQNPITEFVTLDEIDSLNRIVDEFTLDSFTHSYFKNRNFSVKLRKLWSLLEEIYI
                     INPAEMVNCVNNLTEKRVPANFSNIEKNKFINEMTNKFIQKTRKTNIVSDFLFDQVKY
                     DKEHHQLDVLSICGMLLSFVLNAESHLYFVIENIKKTQTVLNTTYDIEEIFSLESKVI
                     QNTKSVIDIHALRNAVSHGAFTVYYDYDIREYFVNFEGILNSYTYNKKYSGLELLILY
                     SNYDKLKDIQELFIRIAFLKATLKLFFLEKH"
     gene            complement(19613..19759)
                     /locus_tag="VF222_04605"
     CDS             complement(19613..19759)
                     /locus_tag="VF222_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141739.1"
                     /translation="MYNRKWSFIVTVIVILSIPITILSSEESSEIGQEIEDNIIRQFT
                     LIDK"
BASE COUNT         7224 a         2854 c         2787 g         7144 t
ORIGIN      
        1 aaatttagtt aactagataa ataaaaaaaa tataagagaa agaatacgat aataatttct
       61 ttattcttat caacttgttg ctatttgtgg attcgcagaa cttccagggt tattgctgat
      121 gtttaccggc gtaccaaatg gttgagtatt agttataaca aagatatcat tgttgccagg
      181 agtgccatca ccccatgtaa catagacatt gttgcctatt gctgctattt gtggcgtgaa
      241 agaatttcca gtattgttac tcaagtttat tggagtacta aatgtctgac tattgtcgct
      301 gcttactgca aaaaagatat cttcgttgcc aggagtgaaa tcataccatg taacatagac
      361 attgttgcct gatactgcta actggtatac tttcgtctga ttcttgattt tgatagtggt
      421 ggtctgtttg gtccattttt gcataaaatt tgatagactg tcatgtgacc aattttgatg
      481 ttatattggt tttgtaaaat ttcctttatc ctggcaggat agtacctatg ttgctttctg
      541 atttgcaata tcttttgttc tacttttttg tatattctat ggatattatg tggtgttctg
      601 gacctttcat ttagtccatc aaaaccattt ttttgatatc tattccaata tctgtagaaa
      661 cttgttctgc aaatgttaat tgtcttacgg atatctgtta tcttccatcc atataatttt
      721 ggtctgatac accatcatct tatctttaca gaatcttttc tatactacac ctggtgtaat
      781 actcctcttt acttgtagta tttgtaggta aaagagtgta acagatgtta tgggataata
      841 caattattga aaaatcaaca atatgtttat atccatattt tcatattttg aggtattgaa
      901 taacgcgggt attaaaataa cgactttaat cattatgacg gtattaacgg gtttagggat
      961 gttacttgca tatccaacaa taaccatttc cgcgcaagaa aacatgacca atcccgatca
     1021 aaacatgacc aatcccgatc aaaacatgac cgcactaggt tctgggacta tatctggtgc
     1081 aggaagagga gctggttaac acaaaatcat atataacaac cagtctctga atgactaatg
     1141 aattctccat taaatttttt tactctccat atcaaaaaac tgcttaattt cggtacaaaa
     1201 aatatacata ttgttatcta tgttcttatc gctgtcctat ccttagacac attgttaaat
     1261 caaacaggtg acttaattgg tcaaccgcta aaaacctttg gaggaattgt tcttttcatc
     1321 accttatcaa taataactat gataggtcag atatttgttc tacaatttgt agctaaaaaa
     1381 agcgaagaga ttaggaaaaa aaatgggaaa ataagattaa tgcatagatt tgtttctata
     1441 agtcaatata caataattac catatttgtg tttcttatct tagaaataat aataaaccag
     1501 aattatttgc cgatatcctc aatgctagtg actctgataa gttacatgct aagtactatc
     1561 ctgatggcca tatttactta cgtatttctt tcatggtata aaactaatcg caattcagtt
     1621 ttagtactcg tatatggatt atcttttgca actgctgcaa tagcatcatt tgcaatactg
     1681 ttagtattta cctatcaatt tagtgagaaa ctttttatct ccatccttcc cacatctcct
     1741 ggaacttttc tttctactga ggaaggttca atctggaaaa tctttgccaa aacttatcaa
     1801 tataatgaca tggcatcttt tttccttaaa tggggtggaa cagcattaat tttatatcat
     1861 tattcttaca agattggacg agcaaaatac tggttcctac tcagtctacc gcttttgtat
     1921 ttttccactt taatggtgta tcatcttcac atctatgaac cacatgaaga atttgaacaa
     1981 ctaatattct acggtgtcgt tgctttgaat tcaacatttg gagggatttt gttttacata
     2041 gcattcaagc ttacagtcaa gaactttaaa ggcaatgaat tatttgcaag ttatttaatt
     2101 atggctggat atggatttat gttattcttt tctggcaccc aatcggttct aacaaatatg
     2161 gcatacccgc cttttggatt ttccactgtt tcctcatacg gtcttggatc atatctgata
     2221 cttgtcggtc tttcattatc ggccatctca atttcaaagg acgaacaatt aaaagaaatg
     2281 attaaaaatt caacaatggc tgaatccaaa tttcttcata gcattggaat gtcagagtcc
     2341 gagcgtgaaa ataaattgtt aaaagatatc gtgatcaaag ccaaacaaca aaaggagaat
     2401 ctagaaaaag acacaggaat agaattatcc atcagtgaag aagatatcaa agaatttgta
     2461 agagaagtag aagtcaatca aaacgacaag gataaggata aggataatga gaataaagag
     2521 gatgaggaaa aaggtaatca ataattctaa gaataataac agaaacataa ttagatcctt
     2581 tgcataatta aaaagcttac agctatattc ctcatttctg caatgattag caccttagag
     2641 aaagaagaat agactacttt ctttttttta acccaaaaag gaagtttatt attaatctac
     2701 aattactact ttttgtttgt taatttcatc aaaatttggg gtatatatgc tacaacagcc
     2761 atgtggtgca attacatctg aaaccttacc aaataaatct ggtcctttat catttaccat
     2821 catgcatttt cctccagtca tatttatcca ataaatacaa ttataacaga aaaatccttt
     2881 ttgaaaattc gaattcttaa actcatcacg ttcaatataa cctgctttga gtttactttg
     2941 tttttgtaat tttgtcacct tccccatagt atctgatgct ttttctgtaa catctccaat
     3001 agtgtctttt gctttgtcaa taaaactttt atcagacatt tctattttca ctttatctcc
     3061 ttttcatttg gtgtccataa agagcatatt gcatatggaa gtattttgcc agaaatatta
     3121 ccgtctaaat cttgaccttc atctgttaca atcatacaat gttcgggttt tataaaatag
     3181 tcacagttat aacagaaata tcctttatga gtatctacat ctttaaactc ctcaatttcc
     3241 atatagccag aggtaacttt tgttaattta ttatgttcag tagcctgttg aggaatcttt
     3301 tgtgtggatg aaaaatttga agacattata ataactgtaa atattatata gtttataaca
     3361 attatgactt gctactttaa tataatctat tattaattaa gacaatcgaa aaattaactg
     3421 taaaagatag aagtgaaccc gagaaaacta cgggcccgtc gggagtaata ccgatagcgg
     3481 tgctaaaaga ggagtccaat gtagagctat cagtacttgt atttagcttt tattcttaga
     3541 aacttatttt aaaatatata taaaaaaagt ttctttaata ttttgtcact tatcttatat
     3601 tcaagatttt ctgttatgga tagcattttt ttggattaat atgaatatca tttgataaaa
     3661 agaaattcct aatttgaatg cctcaaaatc tatgtttctt aaaatgtatt ttattgattc
     3721 attaccagat gaagatagta caaattcatc tataattttt ttgaattttt catcatgatg
     3781 gtgttgaaac attgactttg atttgactta tctacatagt tattaagaca ttggtagaaa
     3841 tgttagtact aatgcctagc atgtagcata atgtctattc ttataattct taaatcaaat
     3901 tttcatagca tttcatttta tttaaatcat ttgccaattc aagtaaggga ttcaatacta
     3961 aaaaatattg taattccaat ataactcaaa taaaataatg agtactttct ataacctata
     4021 tattgataaa aagccatagc catcgtagta ggagccaagg aaatgtagaa ggctatggta
     4081 tgccaaaggt atttggaatg acaggttttt tgtttgtcgt cgagtaaata tcagtgttaa
     4141 ttataatatt taataattga agataattta ttggtaaaag aagaaacaag acacagctta
     4201 ctactaatga ttgatatcaa agaatcatca tcattgttgt tgttggtcta ttactattat
     4261 gtacttcttg gctagataat aaccattcaa tcttattttt taggtctttc aataatactg
     4321 gtttgtataa aacaattttt tcaatatcaa taatactttt ttgtaattgt tgaatatagt
     4381 ttatatctgc agtggtaaaa caaattatta cgttattatc aatttcttta attttgtgat
     4441 ataatgtcat tccatctatc cttggcattt tcaaatcaag tataattaaa tcgtgactat
     4501 tttttttaaa gttgttgaat gcttcaacag gattggtata tgcatctata atatatccat
     4561 tatattctaa aaatattgca aataaattag taatgtcttt atcatcatct attatcatta
     4621 ttcttttgtt tttattagaa agtatctcaa ttcactgcta tagtattcaa tatactatat
     4681 tagcgttgga gacaataatt aactattttt tatcaataac taaaagaaaa tttacatgaa
     4741 caacaaaaaa tttatttgat tagattcttg tagaaatatt ctacatggat aatggaatgg
     4801 gactcactaa tttttgtact tcatctttca aggtttttaa aagtataggc ttttcaatta
     4861 ttttatcaat gcttagctaa gttgtcttta ctgttttatt tctggatttt attttagata
     4921 gaccatattg aataagatat ttttggtcat tgattccttt ggatttgtaa taatatttgc
     4981 taactaaata ggaaatcaca taccacatta tactatttac tataatagta aataaaaaac
     5041 cataatagta tgccataatg cttccagtaa tagtaccacc aattagaaaa gggatttgtc
     5101 tttttattat tgattttaaa attgtatcca aactactatt atatatacaa caccctataa
     5161 aagtagtaat agcagcaatt tttcagtctt tttcctttgt tttgataaag cttactagac
     5221 cattaatttc taaacatgac aatttaatgt cacaaatatc atataagaat tgtagataat
     5281 tctattccta ttaaatattc atattaaata atacatttta tttgtttaac taaattttat
     5341 cttattacaa agaaaccaaa ttaaattgac taatctatat cttaattaaa atcaattact
     5401 tttttctaag ttcccttaat ggtagaattg gtgtgtgaag gtggaaaagc agctggcggt
     5461 agcggcagaa atgatgatta agaatgagaa tatctaaaat gattaataaa taattatctt
     5521 atcttctatt ttgtcatcat tgttcaaact ttcttaatta tccattgtta ttactattca
     5581 cttacttgtt agttcactat tacatttaat aacagagctg atttgaacaa atggtaataa
     5641 agataaacct atacgggtga gacaaggctt gaataagcat aagatgagaa tataatacaa
     5701 aaaattgttg atacaaattc tgttctatgt gtgataatcg aacaaaccca atatagtaaa
     5761 atcattaagg taatattcga atagaaatct tcatttaaag tcaaatatac tatgtgtaaa
     5821 attttaacat gttatgagtc atattaccga tgctaataaa tggagggaag aatccttgaa
     5881 ggggttactt tgtacaaaat ttcaatgcta taacaagcca agaaatcaat gttctgaatg
     5941 ttcaatttat tattgtaatg aacatgtaac atcacatatt catactgata ctttccaaga
     6001 tttacagaga gaacaagaac aagatataga taaaactaga taatgctttt tttacctttg
     6061 aattaataat ttctaaaatt aataaattga gaagaatcaa ttacacaatt acataagaag
     6121 gcttgaatgg caaaaaaaac ctactcttaa agagataaaa aattgtatag atactaagca
     6181 attataattt atttctctta tttttcttct cttatttttc ttctcttatt tttcttacta
     6241 gtttagaaaa attgaaaaaa taataatata aatattaaaa tcttttttag gtattttatc
     6301 ctgctatatg tcttatgtta gcatctttgc attgtccaga acaaaaatta ctactgtagt
     6361 aactattgtt taaagatgaa ccacagttaa gacaattatt tattttatta tcgctcaaac
     6421 tatatcttta caccaccatc tttatcaatc acaatgcaat atcaagtaca ggctattctt
     6481 tagtaacatt ctgttttgct aattgtgtac atacttctaa atgattatgt tcttctttca
     6541 atatctttct aacttgattt ccaaattaaa tttaggttta aataacaaaa ggaaaaggtt
     6601 cattgaggct catgtataac atgtcctcta gtttggtttt tcagtccaaa gaactccaaa
     6661 cagaacctct tgatatacat ttagacaata tagttaatat ctttatttgt tctttaccgg
     6721 taattaggca ctagaagact tgttattttt gtataatctc atttcctagg attgaaaaaa
     6781 ctaaatgttt gtatccttac aagtctagcc tttccctctc tccctctctt aatgtatcta
     6841 tcaaattgat aaataccttt tttgtgtatg cattatgcca aatcttgacc catggataaa
     6901 tcaaaatgag gaatttatgg cggctacaaa tacaataaac aaatttgaaa atcatttgct
     6961 atctattaaa gatgaacatc aaaaaaggat acgacccaat ttacatccta cctataatga
     7021 acgtcatcaa aagattatag aaatgataga ttggagtttg gaaaaatata aagcagcagt
     7081 agccaccaat ataaaaaaga agaaccaaca gaataaagaa gaagtaataa tagacaatat
     7141 catagaggaa ctggataaaa aaagagatat agctagaaat aaaatgaaaa aagctctact
     7201 aaaagatgaa gtgtcaattt atagtttaga ggaaaatact cttgattatg ttcttttcat
     7261 aatctgtgaa ttgacaggta ataaattgta ttaaaataaa aatagaatgt tcatacaagg
     7321 taataattcc tatcctctgt ataataatag atacaaatat catacaacaa aattcttatt
     7381 cactgttgta ggattctacc actactactc cgattggtca gcatattatt acacattgga
     7441 gtaaaggaat tattaaaaaa atttgattct acattcttta attgatttaa aaaattactt
     7501 tgttaaatct tttaatcctt caccaatatt ttcagccatg ttttttgcgc cttcagtaat
     7561 cttggaaccc ataccactag cattttgttg cattgattca cctgtcttat tagctgattc
     7621 acctgcattc tgcatagttt cattggttcc ttctgattgt gcaaatgttg gaatagtaat
     7681 tgtcactaaa gtgaggacaa ttggaagaat tgctactgtt gataatatta gaaacaattt
     7741 attgtatatc aatatcaatc aactactctt tctttgcctt tttctgcttc atattcttcg
     7801 ccagtatctc tatcagggtc ttctactttt ttacctaatg ctctagctcc tgcctttatt
     7861 ttatcatcta accctacttc ttcttctgtt tcgattttta tgtctttatc gttttccatt
     7921 gtaattgtta ttatacatat catgtatata ttcacttaga tttaaaaggc caaacccaag
     7981 ttatcatgta gacttcatat atatgaaaga tttacaaaac acaaatctaa attactgttt
     8041 tttctaacta aattcaacta caatttcttt ctataatcgg aataatcaaa gtaaattcca
     8101 aatataataa attattgatt gcttattcaa tataaaatct tggtattcgt cgtatctatt
     8161 tccatgaaaa tctcaagata attcaattat ggattatcaa gaatcaaagt tgatgaatcc
     8221 cgagaaaagt atgggcacct cgggtagagc tttctatgta gcgggattct actaccttgt
     8281 atttagcttt tattgttaga aacttatttc taaataaatt taagggaatt ataacaatta
     8341 atatttggat gttatagtat ctaaaaaaat attaaattaa ttattctttt tttattgtag
     8401 tagcttgtaa caattgtgcc atttcgttat gcattttaag taactttaat cctttttctg
     8461 tagttttgaa ttggtgagtc ccatcaagat attctattag attgttttct atcaagatag
     8521 aaaggtattc ttttaattgc ccataactta gaaaagctat gtacataatt tttgtcttgg
     8581 ttgttcctcc atttgcagcg tcaaatatat tactctctat ttctgttcgg cttctatttc
     8641 ccatattatt agaaatgcga tttacttatt ttaatataac ctcataggaa aaggtaagtt
     8701 aattatagta aaacattcaa catattattc gtaaataaaa aatataagaa atgttcatgt
     8761 cttaactcaa aattgtatta tgaaataatt attatcttat ttaattttgt caacaattca
     8821 ttctaataaa aatctattat cttaatcctt ttgataaata atataaatat tttctaattg
     8881 aaaaactaaa tctgaataat gaatttggta aatgtagctt ttgtataatc acaaaaaagc
     8941 tttactagtc atatcgtata aaattaagat gattctatct atagaacatg attatgcatg
     9001 gatatcttta ccttctctta tataccttgt aacgtatttg ttaatatgtc aacaaaagaa
     9061 gataaaatta atgaaacaaa cctaaataga gctaaattca atacagataa cactaataca
     9121 accaccaatt tcattgaaag aacaaataga gactacaata acaccttaaa ccaacagcaa
     9181 gatgcaataa acaagacact agacaacacc ttaaataatg taaaaagaac aactgatgaa
     9241 gctacacgag agattccaag atatacacaa cgtattgctg aatatcaaga acaaactatt
     9301 caaactgtaa aagatattgc ttctgacttt attgaagcag aaaaacaagt aataggctct
     9361 tttcaatcac aagtagatag aaatagtgga aataccaacg gattatggaa ctggtttaat
     9421 ccacaaagaa tagcagaaaa tcatgcaaaa atggtaaata actttacaag ttacctgcta
     9481 aatactagca atctaataaa caatgctcta gcatcaaata tgagagtata caatacagca
     9541 ctagaacaaa cacgtgataa tctaaaggct tgtgcaaaaa caaacaccaa tttcatacaa
     9601 tcagttaata gacaaaatag agacggaaat actagcaaca acaacaacaa tatcaacgaa
     9661 agaccaacta ggtattaatt ttaacctagt atataatatg aaattttgaa ttattgtacc
     9721 taatcaatct tcatagtatt ttattaccaa gttaagatat tatcacttta taaatattct
     9781 taataaatga aattcacgtt tgtatattta ggtattcaac tacattgagt attgaatata
     9841 gaaaaagact acttactatc acatatagtg aacccgagaa aactacgggc ccgtcgggag
     9901 taataccgat agcggttcta aaagatgagt ccaatgtaga gatatcagta cttgtattta
     9961 gtttttatta ttagaaactt aatgtttttg aaaattcaga ataaatatca aatctattta
    10021 ttttaggatg ttaacaagat ttcacttctc aaaatcaatt aaaactaatg tatatactaa
    10081 ttatacccat ctaacaatat ttctaaagta tagatagata ggttaagtaa ttgattttaa
    10141 agaaaaaaaa tgttatttca ttgatgtctt ggtacccaac ccacgccttg gaaattattt
    10201 gcaatggcta gttactagca actgtaagtc gctcttctat gttacaggtc agtgattctc
    10261 ctgcaaaaat ttcaccacta ggacaatgtc ctataaaatg ttcagttgga ccgaatatat
    10321 ttacattcaa gtcactctga atattgtcta ttatagtatc aataacttgt gtagatactg
    10381 ttacaaacac aagaaaaaca tctggaccca atgtaactat tacaggaaca ggtgaaccat
    10441 tgaaacgatt aggatttgga tggttaccgg taatatcaat attataaata ttaggaagta
    10501 tttctgtcgt gattctatta catgcctgga cacttctggg attagaatca tctggtgtac
    10561 atttgatagt taaactcaca ctcaaagtag ttggtgttgt cgatttcggt ggtaatgcac
    10621 aatcaattga tgctgggtca acacttgtat tcaatacaca ttgtaatgtt tgattggtta
    10681 gtatgtctct gatgttgttg attgttacct gtgtagcgtt ctgtcctggg gggccttgtg
    10741 ctattgttaa tttacagaat tcgactgatt caacaaaaaa tccttcaaat tgaccagttt
    10801 gacaggcaat tttcttgtct ttagttggat atgtactata actatctccg taggtatcgt
    10861 atccttgagc cattacattt gaaaagatgt tgttaattgt tgctatactg aatagtgtta
    10921 ctagtataga catcagtcca ataattacta acttgtgcat tactttagaa tattaaaaaa
    10981 atatatttgt actgcgaata atagtagagt aagaaattct ataaagcaga ataggtaacg
    11041 actattaagt ttctcattaa ctaattattt tagagataga acagactaaa atcttaaatt
    11101 tataattgac ttttatattc tacgtaaagg cttacacaaa aaatatgggt tatggattgg
    11161 tacgcgtgtt gttgttctca atgatatcaa tccataactt atcgaaaatc taggaattaa
    11221 gatattgtta ttatctaaat atatgtaata atattttatt attaacagta aaaaaatttc
    11281 tcagtatatt tttcaagaat taaaaattat ccttatgagc tcgtccggag tagaatagtc
    11341 catgtagtaa ggttctatta cattgtattt agcttttatt attagaaact taattattat
    11401 attgttgaaa attcagaaat agacaaagga ttatcaaaat acagtataga taaaatacta
    11461 aaatatagaa tatcatttga acaaaagaaa tcaatagaga ttcctttaaa aatagttcag
    11521 tcaatctatt taaaatataa atgagacata accaattttt cggttacgga gaaatcccta
    11581 ttatttttgg tgggtaattt aagaaaatat taacaagaat atatcctata acaataattg
    11641 aaaaacccga gaatgcatag gaaaaggcgt ttaataaagt tttattttta tttattttta
    11701 atacagaaat taatgacatg aaaactaact cataattata tacttataat attggataaa
    11761 tataacaagt atattggtat tagtattttt tatgtttaaa ggatatataa tttaattata
    11821 aatgattctt ataataatat attctacaaa agaaaaaata atagatttag ctctcgcgaa
    11881 attgatatgt gtagattaaa agtatcatta acataatttt atcatttatg atatgaaaaa
    11941 tgaaatttta ttattattac taaatcaatg taaaaatcta ttttaccttg tttctcatta
    12001 atctcaaaag atgagcagga cggcccataa gactgtaaaa gcccagctaa atggtgcagc
    12061 aatcaaaaga taaacagcca aacctaccat taagactatt cctatgatgg acacatatgg
    12121 gaagaatggt gttttataac cataatcaag cttgtctcca tacaaagttg aagccataac
    12181 ctactactat tgagaaacga tgttaagatt aaagacttgt ggtttagaag gtaaaatgtg
    12241 atatttgaac aacaatccgt tcctattttt tataattaga attgattaga aggagataat
    12301 aatgataata aactttatat ctgaatagac caataatttt atattgaatt ataatcaaag
    12361 aaataattct tttaccaatc atcatgactt tcaacattat gttttattat ataatgatac
    12421 aaaaagtcga gatgactatt taattaatta tattaatcaa ggtttaaata aaaatgaact
    12481 ttgtatttat gcatctgttg gtttaagaga taaagatacc ttaaaatttg taaaacgaat
    12541 tgtagattat gagaaaaata tcagtgaaaa taatctaata cttgttgatt tagctccttt
    12601 ctatattgcg gcattaactt acgattttag agtatttaaa gaaactgcaa atgaatttag
    12661 taaaaaaatc aaaaataggg agaataaaaa tatacggttt tcaagtgatg gtgctggttt
    12721 gctatatgat aataaacact ttggtctatg tgaatcaata gaatcatggt ggcaacataa
    12781 aagaccattt gaaggcattg ttctatgccc atatcaaaaa tcaaatcttg agaaatatcc
    12841 ttttaacctt cacaaagata atgctattgg aaaacagcat gacaatttta ttgatgctga
    12901 aagtatttta aataaagaca ggatagcaaa taaaaaggaa ataaatagta gtaaagaaat
    12961 taacaataaa gaagatagga aagaatatgg aggttcaaat cgttaatgcc gccgccatca
    13021 tcatctcaac aatcaacaat caattcaata ggcaacgcac atataatgtt actatatgaa
    13081 agtgatgaaa atcgtaataa cattcttata gattatatta atgaaggcct aaagaatggc
    13141 tgtttttgtt tttatgcatc tgtagatata gacaattcta aaaatatgtc gttaatagat
    13201 agtttatctt ccagaataat aaattatgaa gagaacatcc agaacgagaa tctacaattt
    13261 atcaatttta gaccttacta tgaatctgcg ctcaaaggag atttaacact atttgaagaa
    13321 ttgaaaagag atttagaaaa tatattgtat aagcgtattt ctgaaggaaa aaaagacaaa
    13381 atactattct ttgcggatgc agcaagtcat ttatcggaga ctatgaattt tagccaatcc
    13441 atagatttgg aaaaatggtg gcaagatgtt agcttagagt ggagaagaaa taacaaagat
    13501 attacaatag tatgtccaca tcccaactat atctttaagg acgtatcatt agattataca
    13561 aagtttaaaa ttactgattc tcatgatatg acaattgaca tacaagataa acattcttta
    13621 caatatgtat ataaactgat taaaaacaat agacagataa taagaatact gattgcagaa
    13681 ccagagcctg atatacgtta cctttacaga gaatatttta atggtcttga attagaagta
    13741 gaaattgtag aaaatggtag taaatgtatt gaatatctat tagattcaaa agataaagac
    13801 aaaggagaag aagagggatt tgatatggtc atattggatt cacatcttcc agatacaaat
    13861 ggagtagaag ttttaaaaac tattagagaa aaaatgccat atcaaagaat tgtatttact
    13921 acaactcatc ctttaagtaa aataaatcct gtggtcgatc catttggaat agaggaagat
    13981 gacattctag taaaaccatt cagttttaca aaattacttt ctacaatcaa accaagaaca
    14041 actactaaat agacatttag tcaaagaatt aaatgttgta ctagtatagt tgctgttaaa
    14101 atgatacttt ttattttaca attattacag tacattttga ataatgggca accttgtacg
    14161 aggtactacc taatagaaat tgttgggatg ttttttgtcc tttggtaccc actataattg
    14221 catctacttt ttctgtttct gcaatatcta agattttttc agctggacta tttccttgtt
    14281 cttctataat tttaagcgaa gattttaatc cattactctc tgcttttgtt ttatattttt
    14341 gtaggatttg ctgactatct tgttttaact ggttgaaatt agaatatcct atattaccta
    14401 cattctcaat tatatgtact aaaagtaact gttgtttatg attttgaata atacttgatg
    14461 catattcaaa tgccttgtcg ttatatttag aaccatctac agcaacaaga atttttgata
    14521 gttccaatat ttactatata tacaatactt ttatttttta ttcaaaaaaa tacaatattg
    14581 attaagtata ttaaaatgat atttcgtttt tctgcttatc tattgtataa caataactaa
    14641 caataatatg cgataaataa tcaataatgg ataacatctt ataaactgat tattttattt
    14701 atagaattct ttttataatg tttcaaatta cttaatctta tttaatgctc ttcctcctgt
    14761 tgagacatga gtcacttcta gctctgaaac ttccgctttt tttaatgcac taagagtatc
    14821 tcctcctccg attactgtct ttgaccccct ccaaaaagcc aaagccatac tctttgcaat
    14881 attattagtt ccatttctaa aaagctcaat ttcaaatatt cccataggtt ccaatccaca
    14941 ctatatctat tttcgataaa atataattct cctttatatg agtagtggcc gtttgataat
    15001 aatgaatgga gaatgaattc tacgaaacga ttagtttgtg gcataatgct ttgtcgtaac
    15061 tcaccaataa aacaatgtcc tagatgttta attcattatt gaaactcatt gtaatatcta
    15121 ttacaataat atttattcaa tatatatacc taaaatatat cctactgtat aagctactat
    15181 tgctgctact cccccaatag ccaatgtatg taatccagat tgtaatactg attttgtgac
    15241 aatttttccc ttcactattc ctatcacaaa aaatgaaatt ccagtaaata ctattgaata
    15301 gataaatgca gtactagcgg atattgttaa aattccaata gcataaataa ttataaaagg
    15361 aattaaagga atcaatccaa gcacattaaa agcaacaaaa gtggttatgg cagtatcccg
    15421 tggatttttc ttgtcatcaa tactcccaat ttcttctctc atcatagtgt ctacccagac
    15481 ctttctcttt gatgttatta cttttaccaa gtcttctaac aactcatctt taaatccttt
    15541 atttctataa atctctctta tttcttctct ctcttcttct gttaaattat caattgacca
    15601 ttcttctttt cttctttctt tttcgataat ctccttatgt gtccttgcag atagataatt
    15661 ccctatagac attgaaaacc catcagcaaa tagattagca aaacctagaa ttaatataac
    15721 cgatacaggt aatgaagccc caaatgctcc tgtcacgata gcaaaagttg ttactactcc
    15781 atcagttcca ccataaacaa aatcttcaat agaccaacgc atagaattct tgtgatatta
    15841 taaattaata tttaaaagtg tatttctaat taaccattaa ataggacatg gtagctataa
    15901 agatagttta tataaaaaga aatttctcaa tagatctata ttaaagaatg attggtcacg
    15961 tctcagagtt aattaaaagt taattaaaat ggttttacaa ttgttttgcg ttatcagctc
    16021 ctgtatttga caatttatat gtattgtatt attttcagat aggggtttct tttactttag
    16081 tattttattg aatagttgtg tagaactaaa taaaaaaata gaaaaataat aatgattaaa
    16141 attaagcagt cctaaagtcg aataaaactt taattggact ttctactggt ttactgttaa
    16201 ttgatattat ttcgacactt atcggatatg tagaaccctt tttgaaatct gattggacat
    16261 ggtaagctcc agtactacta aaatctggcc catatgaaat agtttcatta ccttctgcag
    16321 aaattaactc aaggggaagt gattgaccgg cagtcgtaaa taatttagaa aataatattt
    16381 tatcattatt attttcatct tcactgatag taacattata agtaattggg gtatttctat
    16441 ctagtggtat tgtctgtggt tcgatatcta ataagactct tggatgagtt gtaatgtccc
    16501 atactgctcc tgaacctata tctattttaa aaccttcgcc tgtgaaaaat gcatcacttg
    16561 cagtcactgc acccactctc catggatgaa taatacaatg atatagaaat tcaccaggtt
    16621 gtgtaaatgt gaattgaaac aaagagttag ctgtagctgg cattatgcct gaattaaata
    16681 tggagccttc atctgttgct tttggttgtc cactggttac tgtgtgaggc tgtccaaagt
    16741 cattattaaa ccagcctaca gtagtgcctg taggaacggc aatttgtgga ggataataat
    16801 gatgtatgtt ctctttttga gcagcaccag gaactatgct tactgcatag gtagcatcaa
    16861 tatgttgtaa tgtagggata ctaccaggtt gggcaaaggc ttccgttgtt gctacatgta
    16921 ttgtcagagc taacagtatt ggcaatatca atagaatttg attattagcc atattaatca
    16981 tctatagaaa acaaatataa atacacatct attatttgag caatccacgt atcaattttt
    17041 tcttaaacaa ttggtaggat agaggataat aaagaatctc tgaaatatta tgtaatatat
    17101 ctaaagatac aatttctgat aagatagatt aatccagttt tgcaattatt taaaattcga
    17161 ttacatatat tactatatta aaaaatagta aaagtaattg atgtcaaaga atcatcattg
    17221 ttgttttatc atttaatttt tgactataat tttacgaatc tattctagtt gttgttagga
    17281 ttcgagcatt tccaataaaa gacttaaatg gtcagattta atatgttagt tcatgcaaac
    17341 aaccattgct gaaatcatga gtaaaaaaat agaaactgtt gaagaatttg catcggttca
    17401 ggaagttgca ataaagatga aagataagaa tataagctca ttgatagtgg ttgatagttt
    17461 tggtaaatca aagggattag ttactgaacg tgatttggtt agaaaaatct gtgttaaaga
    17521 tgtttgtcct agcaaagtaa taattaaaga aatagtgtct ttaccactcg tcactattaa
    17581 tcctaatgaa tcgccatcaa cagcaattga tataatgtta caacacaata tccgacattt
    17641 agtggttgtt gatgatagta tggaaataaa taaaccaatt ggtatagtga ccccgagaga
    17701 cttaacaaga tatcaggaat ttacaagaga tgatactaat aatgataacc ttgaaaagat
    17761 attagaatat tttatataat atcatttgaa tatttgggta ggttgaattt tatacttcct
    17821 actccacatt ctatgaataa aggttctagt cccaatatat ttaacttatg gtctaaatat
    17881 ttttaattct agaattttat cttaatttct actaataaaa taaagttcta taaatataat
    17941 gaattatata aatagatatt tgttaaataa taagtcataa aatgagtatt agaaatatca
    18001 atatcaatac aattgtttgt tgtcagtaat attttgtaaa tattcgagtc ttatcaatct
    18061 ttttcttctc gtctaatttg atgaggtgta gaagttaatt tttcatttag aactatttca
    18121 tagctttcga taaaatttaa cttctaatat gctgatttac ttttttttaa cattactttc
    18181 aaattgatga acccgagaaa agtacgggcc cgtcgtgagt tgaatgaaca cggtttcagt
    18241 cttaaattgc tatatttata attatatttg gaaaatagtc tttgttttta aatatagatt
    18301 aattagttct attgttgtta gtattaaaat acattctatt acacaaacta aattcaataa
    18361 caaatttaaa ttttttaaaa aattctatta tcaatttaaa caggtacatt aattctaatg
    18421 tattaattca agatgtagat agtcttttac aaaagattca aaatcccatt actgaatttg
    18481 ttacacttga tgaaattgat agcctaaatc gaatagttga cgagtttact ttagattcat
    18541 tcactcactc ttattttaaa aatagaaatt tctccgttaa attaagaaaa ttgtggagct
    18601 tattagaaga gatatatatc ataaatccag cagaaatggt taattgtgta aataatctta
    18661 cagaaaaacg tgttcctgca aactttagta atattgaaaa aaataaattt ataaatgaaa
    18721 tgacaaataa atttattcaa aaaacaagaa aaactaatat cgtatctgat ttcttatttg
    18781 accaggttaa atatgataaa gaacatcatc agttagatgt attgtccata tgtggtatgt
    18841 tattatcttt tgtactgaat gcagaatctc atttatattt tgtaatagaa aatataaaaa
    18901 aaacacaaac agtattgaat acaacttatg atatagagga aatattttca ttagaatcta
    18961 aagtgattca aaataccaaa tcagttatag atattcatgc tcttcgtaat gcagtttctc
    19021 atggtgcatt tactgtttat tatgactacg atataagaga atattttgtt aattttgaag
    19081 gaatcttaaa ttcatatacc tataacaaaa aatatagtgg attggaactt ttgattttat
    19141 actcaaatta tgataaatta aaagatattc aagaattatt tattagaatt gcatttctta
    19201 aagctactct aaaattattt tttctagaaa agcattagaa ttagcaataa ttttatgaac
    19261 aatattacag aatgctctat tttaaaatat acacttaaac agcaggttcc taaaattgag
    19321 ttatatttaa attctaaata atgtcatcaa aaaattatag aatgatagat tggacttaaa
    19381 tatacagcag cagccaatat aaaaaatatg aactagaatg aagaacaagt aataatagat
    19441 aatattattg aagaacttga tagaaaaaga gaagtagcta taaataaaaa gaaaaaagct
    19501 ttactaaaag atgaaagttt ggagtatgga gaagaagaag atattattga ttatgttctt
    19561 ttcataatcc gagaagggac aggtaaattg tattaaaaga aaaataaaag atttatttat
    19621 caatcaaggt aaattgacgt attatattat cttcaatttc ttgtcctatt tcgctagatt
    19681 cttctgaact tagaatagtt attggtatgg aaagaatcac tataacagtt acaataaaag
    19741 accatttcct gttgtacatt atagaatacc aacttctaat gattatagaa tatataaaca
    19801 atattctaaa aaattaacta aactgatatt tctgaattct ttaaataatt ttactaagtg
    19861 gatttaacat catcatttct ttgaatttgc tatttcttgg ttttcctctt aatttatatc
    19921 cacaacatgg gcaataaaat ccctgccaat taataaaaat ttgacaaacc tgacaacgtt
    19981 tctgtcctat tgtataacgt tgtccactt
//
LOCUS       DASVEV010000094         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1586238, whole genome shotgun sequence.
ACCESSION   DASVEV010000094 DASVEV010000000
VERSION     DASVEV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586238"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF222_04610"
     CDS             <1..698
                     /locus_tag="VF222_04610"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX7141740.1"
                     /translation="SSKQEGAVLNVYAMLDLIKSLKWITNNSNITILSDQEVDSKLIF
                     NKNGSNLYDILILGHQEYVTQDEYDNLKKFVSNGGTMILLDGNAFYAQVKHDTSTNQI
                     TLVKGHDWAFNGKSAWKSVDERWKNETSQWVGSNYICALCRVSYLDNPFGYLHHEENY
                     ITNPSDIILLNYPVSYSETNIPKKIVVATYELNYKKGKVIAIGLFSDDIISNGYFDRF
                     FDSLLVKYAFHNN"
     gene            complement(1023..1940)
                     /locus_tag="VF222_04615"
     CDS             complement(1023..1940)
                     /locus_tag="VF222_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7141741.1"
                     /translation="MQQQSENWIILVALWIINGSSFLAIKVAIDTIQPLLSAGLRFSI
                     AGLILFTLFFLRRHHHYEKISREQWKHALVIGAALFLGGQGLLTWGTQYLDSGITGLL
                     NSTIPLWIAIISFLIYRKQMAKSIILGLIAGFAGLMLLIGPSLGAGTLNPIGVIALII
                     SSIAWALGSLYSSKIQLPVSIFASSGMIMLSGGLMLIIVSIMLGEYRDLDLLQISGQS
                     LIAQIYLIGIITVVGFTDFYWLLRVTTPSLANTFAYVSPVIAVFLGWLLLHESITILT
                     IIAMGVILAGVALMVTTPGKRKEHQQIQK"
BASE COUNT          754 a          308 c          298 g          682 t
ORIGIN      
        1 tctcttcaaa acaagaaggt gcagttttga atgtatatgc aatgcttgat ctaataaaaa
       61 gtttaaaatg gataacaaat aattccaata ttacaatact atccgatcaa gaggtggatt
      121 cgaaattaat tttcaataaa aatggatcta atctatatga tattctaatt ttaggacatc
      181 aggagtatgt cactcaggat gaatatgata atttaaaaaa atttgtttct aacggaggaa
      241 cgatgatcct cttggatgga aatgcatttt atgctcaggt caaacatgat acaagtacaa
      301 atcaaataac acttgttaaa ggtcatgact gggcttttaa tggaaagtct gcatggaaaa
      361 gtgtagacga gagatggaaa aatgaaacct ctcaatgggt aggtagtaat tatatatgtg
      421 ctctttgtcg tgtatcatat ctagataatc cttttggata tttacatcat gaagaaaatt
      481 atataaccaa tccatctgat attattttgt taaattatcc cgtttcttat tctgaaacta
      541 acatacctaa aaagattgta gtagcaacat atgaactaaa ttataaaaaa ggaaaagtta
      601 ttgctatagg attattctca gatgatataa taagtaatgg atattttgat agattctttg
      661 atagcttgtt agttaaatat gcatttcata ataattaata tgaagtcatt gcgataataa
      721 taaagtatca aactaaatat gataacataa cttttggaga attgtaaaat cgatacctaa
      781 taatatattt atctaataca tcagcctgta ctcatcttga aatcttctgt gaatgtagaa
      841 aagtcagaat catacttttt aacattttcc attaacatcc agttccaaga acaaatatta
      901 ttattccata cgtttcaaat ggctaacaat acatatataa ttgaatagat aaaatctatc
      961 tgcattatat tgtttgttat atgattggat taaaacaatt aattaatatt tgatattccc
     1021 tttcattttt gtatttgttg atgttctttg cgttttcctg gtgttgtaac cattaatgct
     1081 acccctgcca gaattacacc cattgctatt atagtcaaaa tagttattga ctcatgaagt
     1141 agtaaccaac ctagaaatac tgctattaca ggacttacat aagcaaatgt gttggctaga
     1201 gagggagtag taactctaag tagccaatag aaatcagtaa atccaactac ggttattatt
     1261 cctattaaat aaatttgagc tattaaagat tgaccagaaa tttgcaacaa atctaaatca
     1321 cgatattctc ccaacatgat acttacaata ataagcatta aacctccact aagcattatc
     1381 ataccagacg aagcaaatat gctaactgga agttgaatct tggacgagta aagtgaacca
     1441 agagcccagg ctatagagct aataataaga gctattactc ctataggatt aagtgttcca
     1501 gctcctaaag atggaccaat tagcaacata agtccggcaa atccagctat taatcctaat
     1561 ataattgatt tagccatttg ttttctatat attaaaaatg agattattgc tatccaaaga
     1621 ggaattgtag agtttagcag tccagtaatt ccagaatcaa gatattgagt tccccatgta
     1681 agtaatcctt gaccacctaa aaataatgca gctcctatta ctaaagcatg cttccattgt
     1741 tctctgctaa ttttttcata atgatgatgt ctacgcaaaa aaaataatgt aaaaagaatt
     1801 aatcctgcta ttgaaaatct aagtccagca cttaatagtg gctgaatagt atcaatagca
     1861 actttaatag ctaaaaaact tgaaccattt attatccata gcgctactag aattatccaa
     1921 ttttctgatt gctgttgcaa attaaatcat cttcgaaaat aaaaaggcat attattgctt
     1981 tgaatcatat ttgaaaaaat attatcgaaa attattattc ttcttttgaa ttgttctatc
     2041 at
//
LOCUS       DASVEV010000095         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1197526, whole genome shotgun sequence.
ACCESSION   DASVEV010000095 DASVEV010000000
VERSION     DASVEV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197526"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..355)
                     /locus_tag="VF222_04620"
     CDS             complement(134..355)
                     /locus_tag="VF222_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141742.1"
                     /translation="MGFGEIAANTKKNQQNEAILIDNIIEELDKKRDIAINKMKKALL
                     KDEVSVYRLEENTLDYILFIIREGTGRLH"
     gene            complement(337..540)
                     /locus_tag="VF222_04625"
     CDS             complement(337..540)
                     /locus_tag="VF222_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141743.1"
                     /translation="MPNLDSWISESSNKNEEHITTKTINEFENHLLSIKAEHQERIRP
                     NLHPTYNERHQKIIEMIDWVLEK"
     gene            1269..3254
                     /locus_tag="VF222_04630"
     CDS             1269..3254
                     /locus_tag="VF222_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141744.1"
                     /translation="MKIRSELIVYFLVISLIPLIIIGIFNYISAEQLLKKEILNKLEN
                     IATLGEKNIENVIEENLENIKLISTSLQLKIELDKYNNNIDRNKSQISMNNIINPIKS
                     AIKNVEDISIRDLTGMIVASTNNSAIGKNHTTPDKLFIQDKLQNDVSIFFKDKDNKIK
                     LYVGSPLIHDGKLIGLVTAIYNLDNLLSIFQTYEGLGKTGEFILVQKNDNGDGVLFMN
                     PLRFDSGTAFNLNISKDQINTPTIQSLLKNEKTFTDTLDYRGIPVLAVTRYLEGVDWG
                     LVTKIDKLEAFTPLENLKYVTIINGILVGILVIIASLIIGKSISNPIQKLMAASKNIS
                     QGKFDKSISIIGRSEEIKELSNQFDNMRQNILYTNNHLNDLVDERTQKLKKGVVELKD
                     KEEALKESNKNLLSVNEKLSLQSKSQMEFVNITAHELRTPMMPIITLTETLYSKIKKE
                     NKTQKKNPSKENEKKQEFLEVILRNCYRLYRITEDILDVTKIESQTLKLNKEMIELNE
                     IIRNVVNDFKKIINEKRYGSIQVRIVYEPSKDIILVNADKGRLNQLLSNLLDNALKFT
                     KEGNIIIAAKKQKDDEVIVSIKDSGTGINPDILPRLFTKFATKSEQGTGLGLYISKNI
                     TEAHGGRIWAEGNPEGGSTFYFTLSIVKSDKIEESEK"
     gene            3262..3648
                     /locus_tag="VF222_04635"
     CDS             3262..3648
                     /locus_tag="VF222_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141745.1"
                     /translation="MTTLKKILVVDNEADITYALENALENYGYSIDSFNDSMLALNSY
                     KSNFYDLVILDIKMPKIDGFELYNKIKEKDPNVIICFLTASELFYEEFRKTRYMLGEK
                     LGDEYFIQKPIKTDDLVQKMNVLTNK"
     gene            3737..3952
                     /locus_tag="VF222_04640"
     CDS             3737..3952
                     /locus_tag="VF222_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141746.1"
                     /translation="MILIIIKHYGNSSTTGNDYIIFGQGDGLIPLLGAVPRGFINVGI
                     SDNCHTLTFYLNNKNMTGQNKYYYQNR"
     gene            4074..>4320
                     /locus_tag="VF222_04645"
     CDS             4074..>4320
                     /locus_tag="VF222_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141747.1"
                     /translation="MKNFSSKGIRFSGDSIKSCVGFIDLVDSTKNTITISNLESIRKY
                     YSTFINSISEYVKSYNGKVIKNIGDCLLFYFPKTTDFK"
BASE COUNT         1682 a          569 c          641 g         1428 t
ORIGIN      
        1 ataatagaat ctttaacctt ttctttaatt gccttaatca tttgaggata taaccactct
       61 ttatctactt ttgaaatttg tgggtcataa aaaatcacga cattaactat ctatttttcc
      121 ttttattttt attttaatgc aatctacctg tcccttcacg gattatgaaa agaatataat
      181 caagagtgtt ttcctctaat ctataaacag atacctcatc ttttagcaga gcttttttca
      241 ttttgtttat agctatatcc ctttttttat ccagttcttc aataatatta tctattagta
      301 ttgcttcatt ctgttggttc ttttttgtat tggctgctat ttctccaaaa cccaatctat
      361 catttctata attttttgat gacgttcatt ataggtagga tgtaaattag gtcttattcg
      421 ctcctgatgt tcagctttaa tagatagtaa atgattttca aattcattta ttgtttttgt
      481 agtaatatgt tcttcatttt tattactact ttcactaatc catgagtcaa gatttggcaa
      541 tttttacaaa atacacaaaa aggtcattta ttaatttgat agatacatta agagagggaa
      601 agagggaaag gctagacttg taaagataca aacatttagt tttttcaatc ctagtaattg
      661 tgattataaa aaataacaac aacaaaacta ataccaagtt ataatgatag gataataaaa
      721 aatatataac aactacataa aattaggttt acaagaatta ataaatacaa taattacgat
      781 gataatacta caacaaaata tctcccatat ctcaaccaac caatcaatca catcacttta
      841 tttgtcatag tcggaacatt tcgtaataac caatccagcc taatagcata actactctta
      901 ttaatcttgg ttaataattt gatataaatg aggatcatat atttgattat agtctttggt
      961 aatttatcat agcaatatag acgtattatg gaaaaattat tgaatcaata ttagttaaag
     1021 gataagattt atcgagttct cagcagtgaa attgtcaata ttgtagggtt ttataattat
     1081 catttatcag ttttaagtaa cacatgtgaa aaaaggaaaa tgtggaagct ataatcatag
     1141 atgcagagaa aaaaataggc tagtaaatca ttgaaagatc ctacaagatt taataggaga
     1201 ggtgattcaa ctgcttatat attcataggc tcttttatat ctcataataa tgaaataata
     1261 agctgaatat gaaaattagg tccgagctta ttgtttattt tttagtaata tctctaattc
     1321 cactgattat tatcggcatt tttaattata ttagcgctga gcaactgctt aagaaggaga
     1381 tattgaataa actcgaaaat attgctacat taggggagaa aaatatagaa aatgtaattg
     1441 aggaaaacct tgaaaatata aaactaattt ctacaagctt acaacttaaa atcgagttag
     1501 ataaatacaa taacaatatt gatagaaaca aatcacaaat ttcaatgaat aatataataa
     1561 atcctataaa atcagcaatt aaaaatgttg aggatatctc tatacgtgac ttaactggca
     1621 tgatagttgc ttctacaaat aattctgcta taggtaaaaa tcatacaacc cccgataaac
     1681 ttttcatcca agataaactt caaaatgatg tatctatctt ttttaaagat aaggataaca
     1741 aaataaaatt atatgtgggt agtcccctta tacacgatgg caaattaata ggtttagtga
     1801 ctgccattta taatttagac aacttattat cgatatttca aacttatgaa ggattaggta
     1861 aaactggcga atttatttta gtacagaaga atgataatgg cgatggtgtt ctttttatga
     1921 atccattacg atttgattcc ggtacagctt tcaacttaaa tattagtaaa gatcaaataa
     1981 atacaccaac aattcaatca cttttaaaaa atgagaaaac atttacagac acgttggatt
     2041 atcgtggtat accggtgttg gctgtaacta gatatctaga aggtgttgat tggggtctag
     2101 ttacaaagat agataaatta gaggctttta ctcctcttga gaatctaaaa tatgtgacta
     2161 ttattaatgg tattttggta ggtattttgg tgataattgc atccttgatc attggtaaat
     2221 caatttcaaa tcccatccag aaattaatgg ctgcatcaaa gaatattagt caaggaaagt
     2281 ttgataagtc tattagcatt ataggtcgta gcgaggagat caaagaatta tctaatcaat
     2341 ttgataatat gagacaaaat atactttaca caaataatca tctaaatgac cttgtagatg
     2401 aaagaacaca aaaattaaaa aaaggagtag tagaattaaa agataaggaa gaagcattaa
     2461 aagaatctaa caagaacctt ctttcagtta atgaaaaatt aagtcttcaa agtaaaagcc
     2521 agatggaatt tgttaatata acagctcatg aactccgaac tccaatgatg cctattatta
     2581 cccttacaga aacactttat agtaaaatca agaaagaaaa taaaacccaa aaaaagaatc
     2641 catcaaaaga aaacgaaaaa aaacaagaat tccttgaagt cattcttaga aattgttaca
     2701 ggttatatcg aattacagaa gatatactag atgtaacaaa gatagaaagt caaaccctta
     2761 aattgaataa agaaatgatt gagttaaatg aaattataag aaacgtcgta aatgatttta
     2821 aaaaaattat aaacgaaaaa agatatggga gtattcaggt tagaatagtg tatgagccaa
     2881 gcaaagacat tattcttgtt aacgcagata aagggagatt aaatcagtta ctttctaatc
     2941 ttcttgataa tgctcttaaa tttactaaag aaggaaacat aattattgct gcaaaaaagc
     3001 aaaaagatga tgaagttatt gtgagcataa aagacagtgg cactggtata aatcctgata
     3061 tattaccacg attattcaca aaatttgcga ccaaatctga acagggtacc ggattgggat
     3121 tatatatttc caagaatata acagaagctc atggcggtag aatatgggca gagggcaatc
     3181 ctgaaggtgg atctactttt tattttactt tgtctatagt aaaatctgac aaaatagagg
     3241 aatctgaaaa atgaatataa aatgacaacc ttaaaaaaaa tccttgtagt agataacgag
     3301 gcggatataa cttatgcatt agaaaatgca ttggaaaatt atggatatag catagattcc
     3361 tttaacgact ctatgttggc gttaaactct tacaagagca atttttatga tttagtgatt
     3421 ctagatatta aaatgccaaa aattgacgga tttgaattat ataataagat aaaggagaaa
     3481 gatccaaacg tcataatctg ctttttgacc gcaagtgaat tgttttatga agaattcaga
     3541 aaaacacgat atatgttagg tgaaaaactc ggtgacgaat attttattca gaaaccaatc
     3601 aaaaccgatg atctggttca aaaaatgaat gtattaacga ataaataaaa aatagataat
     3661 agattgactt taaaaaagta tcataattaa aacatacttt ttaggcgccg aaaaattagt
     3721 ttttatcgaa tttattatga ttctaattat catcaagcat tatggcaact cttccactac
     3781 gggaaatgat tatataattt ttggacaagg cgatggatta attccgctat tgggtgctgt
     3841 accacgagga tttataaatg tgggaatatc tgataattgt catactctaa ccttttatct
     3901 gaacaacaag aatatgactg gacaaaacaa atattattac caaaatagat agataaaatt
     3961 agataatagg attagtattt ggataaatct taagttttaa aaatcaagac atttgaataa
     4021 attttttcct accaaatgtc ctatataata ccaatctgat ctaataaata ccattgaaaa
     4081 atttttcttc taaaggtatt agattttctg gcgattcaat aaagagttgt gtaggtttta
     4141 ttgatttggt agattcaact aaaaatacca ttacaatatc taatttagaa tcaattagaa
     4201 aatattattc tacatttata aattcaatat cagaatatgt taaaagttat aacggaaaag
     4261 ttatcaaaaa tattggtgat tgtttattat tctactttcc caaaactact gattttaagg
//
LOCUS       DASVEV010000096         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1004564, whole genome shotgun sequence.
ACCESSION   DASVEV010000096 DASVEV010000000
VERSION     DASVEV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004564"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1118)
                     /locus_tag="VF222_04650"
     CDS             complement(144..1118)
                     /locus_tag="VF222_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX7141748.1"
                     /translation="MTKLKFGLTIPQGWWSDLPLNDPIKQYEFSKKISRSADRLNFYG
                     LYAYDHFIPHHEYNINNSFFECFTLLSSISSITKKIRLGQIVTCNSYRNPALLAKILS
                     TLDVISKGRVELGIGAGWYQNEYSSYGYNFPSNVERIKQLDESISIIKAMWKYERASF
                     QGNYFSINEAICKPHPIQKPHPRIIVGGTGEKYLLKIVAKHADTYNNPFGSPLEIRKK
                     LTVLKEHCNDLRKSYNDIELSVLLPCIIQESEKDVLMRINRWKKKNENISAFLERKSG
                     GYTIGTPEKILLGLQQYIDLGITHFILNFIGIEEDMLHLFDSRVIKKL"
     gene            complement(1145..2743)
                     /locus_tag="VF222_04655"
     CDS             complement(1145..2743)
                     /locus_tag="VF222_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX7141749.1"
                     /translation="MNQNIKIAKQFHETTKHSPVTIYFNRHYLDWENKPFPFKLYLNK
                     PKVNLPSTFPYPIEDSIECLKQQTNLNLNEEKQIDLKTLSQILFFSAGITRKIKLNDN
                     VYYMRAASATGALYPIEIYLICQNLQNLDAGVYHFCPGDFTLTQLRKGDFRSLLFTAT
                     GNNKKVFQAPITLIFTSISWRNSWKYQARSYRHWFWDAGVILANFLALCNNNHLSSEI
                     ILGYQDKLINNLLGLQDQKEVAIALASIGSGCNSDYYNQLNSSELSALNCETKPISKY
                     EETVYPEIWKIHESSYLSTMTEITNWTLKNKDDRNLHNTGKVPYDKISIINTTNIGNL
                     ILQRGSTRRFSKKPITLSQLQTILYLSSISVPFDFITNEQSLIDIYFIANSVTNLQSG
                     MYYYNKLENNIEQLKEGDFREQSGHLCLDQKLFSDASVVFFLLSSMDKILKYLGNRGY
                     RAIQLEGGICAGKVYLTSYSQGLGASGSTFYDDEVTETFLPHSYQKSTIIAIGLGIPA
                     YVSKPGKIYTNVLNKEDYSKKFNY"
BASE COUNT          931 a          417 c          420 g         1087 t
ORIGIN      
        1 ttgtaaaaac tattgctaaa tatataatta ttataacaag atctaactat ttacttaata
       61 ttatataata tttatagtta ttatatgaga tttagaatga atgtgaatta atcattgatg
      121 ctaatcaata atgattatca acttcataat ttcttgataa ctctggaatc aaataaatgc
      181 agcatatcct cttctatacc tataaaattt agaataaaat gtgttatccc taaatcaata
      241 tattgttgaa gtcctaacaa aattttttcg ggtgttccaa ttgtataacc tccgcttttt
      301 ctttccaaaa aagcagaaat attttcattt tttttcttcc atctatttat tctcattaga
      361 acatcttttt ccgattcttg aataatacaa ggaagcaata cagataactc aatatcgtta
      421 taactttttc tcaaatcatt acaatgttct tttaaaactg taagtttttt tcttatctcg
      481 agtggactac caaacggatt attatatgta tcagcatgct tcgctactat tttcaagagg
      541 tatttttctc cggttccacc tacaatgatt cgaggatgag gtttttgtat agggtgaggc
      601 ttgcaaatag cttcattaat tgaaaaataa ttaccttgaa atgaggctct ttcatatttc
      661 cacattgcct ttattatgga tatggattca tccagttgtt tgattctttc cacgttagaa
      721 ggaaagttat atccatatga ggaatattca ttttgatacc aaccagctcc tattcccaat
      781 tcaacacgtc ctttggaaat aacatccaga gttgaaagga tctttgctaa taatgcagga
      841 ttacggtaag aattacaagt aacaatttgt cccaatctaa tttttttggt aatagaagaa
      901 attgacgata acagtgtgaa acattcaaaa aaactattat ttatgttata ttcatgatgt
      961 ggaataaaat gatcataggc atataaacca tagaaattta gtctatctgc tgatcttgaa
     1021 atttttttag aaaattcata ttgttttata ggatcattta acggtagatc tgaccaccag
     1081 ccttgtggga ttgtcaatcc aaactttaat ttcgtcatta ttttattctt tgttattact
     1141 gttgttaata attaaatttt ttagaatagt cttctttgtt taacacatta gtatatatct
     1201 ttccaggttt agatacataa gcaggaatac ctagacctat agctattatt gtactttttt
     1261 ggtatgagtg aggaagaaaa gtttctgtta cttcatcatc ataaaaggtt gaaccggaag
     1321 cgcccaatcc ttgagaatat gaagtaaggt atacttttcc agcacatatt cctccttcta
     1381 gttgtatagc tcgataacct ctatttccta agtattttaa tatcttatcc atacttgata
     1441 aaagaaaaaa tactacactc gcatcactaa ataacttttg atctagacat aaatgtcctg
     1501 attgttccct aaaatcacct tctttgagtt gttcgatatt attttctaat ttattgtaat
     1561 aatacatacc agattgtaaa ttcgtgacag aatttgctat aaagtaaata tcaatcaggc
     1621 tttgttcatt ggtaataaaa tcaaaaggta cactgatact agaaagatat aaaatagttt
     1681 gtagttggct cagggttatg ggtttttttg aaaatcttct tgttgatcca cgttgtaaaa
     1741 ttaggttccc aatattagta gtgttaataa tactaatttt atcataagga acttttcctg
     1801 tgttgtgtaa atttctatca tctttatttt tcaaagtcca atttgttatt tctgtcatgg
     1861 tactgagata agaagattca tgtattttcc atatttctgg ataaaccgtt tcttcatatt
     1921 ttgaaatggg tttagtttca caattaagag ctgaaagttc actggaattc aattgattat
     1981 aataatcaga attgcaaccg cttccaattg aagctaatgc aatagctact tctttttgat
     2041 cctgtaatcc tagtaaatta ttaatgagtt tatcttgata tcctaaaata atttcggatg
     2101 ataaatgatt attgttacat aaagccaaaa agttagcgag aattacacct gcatcccaaa
     2161 accagtgtcg atatgaacgt gcttgatatt tccatgagtt tctccaagat attgacgtaa
     2221 aaattaaggt aattggagct tgaaatactt tcttgttatt tccagtggct gtgaatagta
     2281 gtgatctaaa atcgcctttt cttaactgag ttaatgtaaa atctcctgga caaaagtgat
     2341 acacacctgc atctagattt tggagatttt gacaaattaa ataaatttca attgggtata
     2401 aagcacctgt agccgaggct gccctcatat aatatacatt atcatttaat tttatttttc
     2461 gtgtaattcc tgcagaaaaa aaaagtatct gagataaagt ttttagatct atttgtttct
     2521 cttcatttag attcaaattt gtttgttgtt tcaaacattc gatagaatct tcaattggat
     2581 atggaaatgt agatggtaaa tttactttag gtttatttaa atatagttta aagggaaacg
     2641 gtttattttc ccaatctaga taatgacggt taaaatagat agttacagga gagtgttttg
     2701 tggtttcatg aaactgtttt gcaattttta tattttgatt caaggatata tgctaaatta
     2761 atatcataaa aaaattattt aaagaggttt gttacttgtc cccatatctg atctaagaaa
     2821 ttttttccta tttccatggg atctttcgta ggtgt
//
LOCUS       DASVEV010000097         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_616605, whole genome shotgun sequence.
ACCESSION   DASVEV010000097 DASVEV010000000
VERSION     DASVEV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616605"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VF222_04660"
     CDS             <1..563
                     /locus_tag="VF222_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7141750.1"
                     /translation="TLTLSMKIVLTITTLIAILAIGLLGISNSSSNIGNVFAQMDPEN
                     PPVMEEDKNISIMTMSTNQSEPEMMNWTGTIDVESTIGEAFKSKVNVNMIDAIKAALV
                     NVGLNSTVKEAELTHVQNYLVYNIMVIDDDDMKKYIVIVDPGNGEVLKKKEITWCDEH
                     DKMKYGNEKGDNYGHDNKMMMGDKKY"
     gene            788..1246
                     /locus_tag="VF222_04665"
     CDS             788..1246
                     /locus_tag="VF222_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7141751.1"
                     /translation="MNFPTDKKENPKEKENVDFAAILTGREEVPVVDTLDTALATFQS
                     REEKEDLKYSVKVTDTDKIKEVHIDIGKPVEKADIVAELYKSETPSSEVMGNLCEGNI
                     NSQQLKGPLKGKNTQELIKKIEKGEAYVDIVTEDNPNGKVRGKIKKLTSS"
     gene            complement(1340..1630)
                     /locus_tag="VF222_04670"
     CDS             complement(1340..1630)
                     /locus_tag="VF222_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141752.1"
                     /translation="MIYNRLSLVLITTGFLTLILILTTFSVSTFALSNGTSQTEQNIV
                     KETNQTLISPSSNETGQAIPIQKNATDLGGNITKGAKNLVAKIGEGIENLSK"
     gene            complement(1780..2013)
                     /locus_tag="VF222_04675"
     CDS             complement(1780..2013)
                     /locus_tag="VF222_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141753.1"
                     /translation="MRNKSILITLFLIHPLQGSFAQINNTSIDQLENTTTTITTEDSI
                     VEDKLEQVQNHYNDAANIMEDLVAKEEPKKSSV"
BASE COUNT         1009 a          330 c          423 g          967 t
ORIGIN      
        1 atacgctaac tttatcaatg aaaatagttt taacaattac aaccttgatt gctattttag
       61 caataggttt gttaggtatc agcaatagta gtagcaatat aggaaatgta tttgctcaaa
      121 tggatccaga aaatccacca gttatggaag aagataagaa tatttcaata atgacaatgt
      181 caacaaatca atctgaacca gagatgatga attggactgg aacaattgat gtagaatcga
      241 ctataggaga agccttcaaa tcaaaagtaa atgttaacat gatagatgca ataaaagcag
      301 cgctagtaaa tgttggatta aattctacag tcaaggaagc agaattaact catgtacaaa
      361 actatctagt ctacaatata atggttatag atgatgatga tatgaaaaaa tatatagtca
      421 tagtagatcc aggcaatgga gaagttttga agaagaaaga aattacatgg tgtgatgagc
      481 atgataaaat gaaatatggc aatgaaaaag gtgacaatta tggtcacgac aataaaatga
      541 tgatgggaga taagaaatac taaataaata attgactttt aataattaaa atttaaatta
      601 ctactatttt tttgattatc ttaacttgct ttttttcctg ctttggcaca ttcttctaga
      661 tagatttctt tacattaatt agatgtagtt gtatgataga agaatattat acttccagaa
      721 ttctaaaatt tgattagtaa tcaacttctg aaactattat aagaaatgtt atataatttt
      781 aactacaatg aattttccta ccgataaaaa agaaaatccc aaggaaaagg aaaatgtaga
      841 ttttgctgca atacttactg gaagagagga agttccagtg gtagatacat tggatacggc
      901 tttagcaacc tttcaatcaa gggaggaaaa ggaggattta aaatattctg ttaaagttac
      961 agatacggat aaaataaaag aagttcatat tgatatagga aaaccagttg aaaaagcaga
     1021 tatagtagct gagttgtata aatctgaaac tccatcctct gaagttatgg gcaacctatg
     1081 tgaaggaaat ataaattcac aacaactaaa aggtccgcta aagggaaaga atacccaaga
     1141 attaatcaaa aaaatagaga aaggagaagc ctatgtagat atagtaacag aggacaatcc
     1201 aaatggcaaa gtaagaggaa aaattaaaaa attaacatct tcctgagtta aacttctcgt
     1261 gaatcttatt ttcttacaga aataattact ctataagtaa tatcttgtca gtaaaaaata
     1321 atttaagata aaattgaatt tacttggata aattctctat tccttcacca atctttgcaa
     1381 ccaagttctt tgctccttta gtgatgttgc ctccaagatc agtagcattt ttttgtatcg
     1441 ggattgcctg accggtctcg ttggaagatg gacttatcaa cgtctgattt gtttctttta
     1501 ctatattctg ttcagtttga cttgtgccat tagataatgc aaacgttgaa acactgaacg
     1561 ttgttagaat aagtattaag gtaagaaatc ctgttgttat caatactaaa gataatctat
     1621 tgtatatcaa ttgtcattct tttaaattct attttatata aaaatagttg ttattataac
     1681 attagatatc tctatcaaaa tattttgtaa gatatactat ttaagaagat caagataata
     1741 atcgattgaa gaaatctttt tgactaacta tatattcaat cacacactac ttttctttgg
     1801 ttcttctttt gctactaaat cttccataat atttgcagca tcattataat gattttgaac
     1861 ttgttctaat ttatcttcta caattgaatc ctccgtagta atagtagttg ttgtattttc
     1921 taattggtca attgatgtat tatttatctg tgcgaaagat ccttgtaaag gatgtattaa
     1981 aaataatgtg attaaaattg atttatttct catttgtaat tatgaatatt acattttact
     2041 tcaacatttc ttatcgttgt tatttttttg aaaaattata ttttttatca ttggaataag
     2101 gaattcaatc attttcattt cataaaatat ctttgggatt gttgtagtaa atttttttat
     2161 catttttata gactacatat ataatagcta ataatttgag atatttttct ttagataaaa
     2221 aaaccataaa ccatctaatg aacaatctgt ctagaaagct atgacgaaat ggtaagataa
     2281 gtgtttttca tggtttggtc atcaatggtt ttgtcgttaa tgtaaatcaa tggggataca
     2341 tcaattcaaa agaaatgatg ttgttaagta atagatgcta agataatgtt taacacatat
     2401 atctaacatc tatacagttt ttaggattta catccctact tctgtaggtt aatatgttct
     2461 acaaagtata taataatttg acacaattta tcttattgtt aagcaacatt taacatataa
     2521 taattataat agttttttat tatgttaact atacttaacc attatgagat atgaagataa
     2581 ttcttatgct atttctattg agaatgtttc agaagaatta gatgatcatt ttaagaattt
     2641 tggaaaatat gctagtgatg tgaattataa tctatttggg tattgcaatt tttgtaatac
     2701 tagaatagat gaatttggtt actgtgctt
//
LOCUS       DASVEV010000098         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1394257, whole genome shotgun sequence.
ACCESSION   DASVEV010000098 DASVEV010000000
VERSION     DASVEV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394257"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VF222_04680"
     CDS             <1..961
                     /locus_tag="VF222_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX7141754.1"
                     /translation="VTLNIPFIIYGNIVLEYSSLESVHVALNRLAEQGNVIGLISEIE
                     INMKEDKRKYPLFKKVPFYIKDSLEDYKKSSSEGLVLEYKDESSLKMLQNCRKEFDGP
                     ILVDVSDVFPLYIKTILEEGADGIIVDTEKVTKQNNRYRGKHAIAVIYDARNAINDYY
                     IGRAEKNDNDDDDDEDDNAILVVAGDVNSAGKIVKAAALGANVVGYSTSMLIAHSHMY
                     SEKPADVSSIAENVYRHMIGTKGEIKGIPAALGYSDFHNLSPSDLRTSSIQASIQGDI
                     PIEGIDRTYIEMIEEVINEYVSETGIKLNSLETQEVLRSMVRD"
     gene            965..1840
                     /locus_tag="VF222_04685"
     CDS             965..1840
                     /locus_tag="VF222_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141755.1"
                     /translation="MCGICAVMLENRKKDFPIGCLLYKMMESQQIRAQDSTGVAIFNN
                     GSTKEFYRIDYFRKNDGVYNTFTEKVPPERLDKTLYDLTQDPNIVIASFSKKMNLLKD
                     IGLVRDLESKYKIRSIQGTHGIGHLRIATSSRVTPDNAHPFSTIVMPDIAVVHNGEIT
                     NYAKLRSLLELQGYRLFTRCDSEVIAVFIADQLLRHGDLEKANYELIKKADGPFTYIV
                     STPDSLALVRDKFGARKGIFGYNPGNNDYPSFWAMATDLSALDSVGATFYVETPEPGI
                     PKMFYNEMKIRSIPN"
     gene            2485..3393
                     /locus_tag="VF222_04690"
     CDS             2485..3393
                     /locus_tag="VF222_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141756.1"
                     /translation="MYILDIDEIRSIADSIGSNTSNLDEQFERLDFILYEKYRINLPK
                     GLKKASLEKFRDIISSPLLYERAVNSILKRKIIEGENKITIKNPKALYRIGILSFREP
                     VKLDLVVEGSVGNFFAAGCNFNGTWTVKGHAENGLADKGYSGKIVVDGLATELAGQNN
                     QATEDSNGVDILVRKGCMERAMGQARGGSLITFGAGYNSGLYMSGGVLLNLGAPGKSF
                     GPGMVGGTIYSPIGTTVGEGAAIRNMSKTDYKVIFSVLKKFETELEIKGLDKFSPENN
                     ILNITNNGNSEEYNFRNFIKINSTEL"
     gene            4005..4232
                     /locus_tag="VF222_04695"
     CDS             4005..4232
                     /locus_tag="VF222_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141757.1"
                     /translation="MLSILLLVSIDILSYNHIALGSSDSNGDNDDNDNDNDNNNDNDN
                     DNNNGGEKEEKEKDNDHDNDDVPFILPVPFP"
BASE COUNT         1592 a          514 c          802 g         1373 t
ORIGIN      
        1 agttactttg aatatacctt ttataatata tggaaatatt gttcttgagt atagttcttt
       61 agaatctgtg catgtagcat taaaccggct tgctgaacaa ggaaatgtaa tagggttgat
      121 ttcagaaatc gaaataaata tgaaggaaga taaaagaaaa tatccattat tcaaaaaagt
      181 accattttat ataaaagata gtttggaaga ttataaaaaa tcttcttcag aagggctagt
      241 tttagaatat aaagatgaat cctctttgaa gatgctccag aattgtagaa aagaatttga
      301 tggaccaata ttggtagatg tgagtgatgt ttttcctttg tatattaaaa caatactaga
      361 agagggagct gatggaataa tagttgatac cgaaaaggta acaaaacaaa ataatagata
      421 cagaggaaaa catgcaatag cagtgattta tgatgcaagg aatgctataa acgactatta
      481 cataggaaga gcagaaaaaa atgataatga tgatgatgat gatgaagatg ataatgccat
      541 attggtagtt gcaggtgatg tgaacagtgc aggcaagatc gtaaaagcag cagcacttgg
      601 agcaaatgtc gtaggatact ctacaagtat gttgatagca cattctcaca tgtattctga
      661 aaaacctgct gacgtcagtt ctattgcaga gaatgtatat cgtcatatga taggaacaaa
      721 gggtgaaatt aaaggaattc ctgctgcttt aggatacagt gattttcata atctatctcc
      781 ttctgatttg cgtacatcaa gtatacaggc aagcatacaa ggtgacattc caattgaagg
      841 aatagataga acttatatcg agatgataga agaggttatt aacgagtacg ttagtgaaac
      901 gggaataaaa ctcaattctt tagaaactca agaagttctt cgatctatgg taagggacta
      961 aaatatgtgt ggaatatgtg ctgtgatgct agaaaatcgt aaaaaggact ttcccattgg
     1021 gtgtcttctc tataaaatga tggaaagcca gcagataagg gctcaggata gtacaggtgt
     1081 ggctatcttt aataatggat caacaaaaga gttttacaga atagattatt ttagaaaaaa
     1141 tgacggtgta tacaatacat tcacagaaaa agtacctcca gagagattgg ataaaacttt
     1201 gtatgactta actcaagatc caaatatagt aatcgcaagt ttttcaaaaa aaatgaacct
     1261 actaaaagat attgggcttg taagagacct tgaatccaag tataaaataa gatcaattca
     1321 gggaacccat ggaatagggc atttgagaat agcaacgagt agtagagtta ctccagataa
     1381 tgcacatcca ttcagtacta tagtgatgcc tgatatagca gtggtacata atggggaaat
     1441 aactaactat gccaaattaa ggtctttact agaacttcaa ggatatcgtt tatttactag
     1501 atgtgattcg gaggtgattg ccgtatttat tgcagatcag cttttgaggc atggtgatct
     1561 agaaaaggca aattatgaat taataaaaaa ggcagatggt ccatttacat atatagtttc
     1621 tacaccagat tctcttgcct tggtaagaga taaatttgga gcaagaaaag gtattttcgg
     1681 ctataatcca ggcaataatg actatccatc tttctgggct atggctactg atttgtctgc
     1741 tttggattca gtaggagcaa cattttatgt agaaactcca gagcctggaa tacctaaaat
     1801 gttttacaac gagatgaaaa tcagatccat acctaattaa ctgaggtact ttattttaaa
     1861 attttttttg agacaataaa ttgaatttta ctgtgtgttt taaaatccta tgttttgatt
     1921 ccttataaat ttaaagagta gatacctata tacaatactt gtccatacaa aggatacaaa
     1981 gattctattc cttctcttga gaaaatagac ttgtgattaa ggggtaaatt tgctgttctg
     2041 aagattcttg tttaattaat gtctttaaca ttactctgta agtttatata agatcttgta
     2101 ctatgtccag tttttttata atgtattgtg atatgttcta aatatttatt catatggata
     2161 aattatttta ttgtgattac tataggagtc tttaaacagt ttgttaaaat atataaaaat
     2221 tatggtagac ctttaaaata ttgtttattt atgtagaaac atatcacaat ttattttata
     2281 agttaaaaaa ttatgcaaat ggtctgatat ctaatgtttt catctttttt tatatggaat
     2341 caaaatttat tctctctagc agcaaatgag ttaggataaa aaagaaagaa ttttacaaga
     2401 taattaagta tagatggtta tatcaatgta gtttgataga ataaaagata tatctataac
     2461 tttgctaaat attggagata aatagtgtat attctagata ttgatgaaat aagatcaatt
     2521 gctgattcta taggttctaa tacatcaaat ctagatgagc aatttgaacg actcgatttt
     2581 atattatatg aaaaatatag aataaatctt cctaaaggac taaaaaaggc ttctttagaa
     2641 aaattcaggg atattatcag ttcaccattg ttatatgaaa gagcagtaaa ttcaatacta
     2701 aaaaggaaaa taatcgaagg cgaaaataaa attacgataa aaaatcctaa agccctttat
     2761 agaattggaa ttctcagttt cagagaacca gtaaaacttg atctagtagt agaggggagt
     2821 gttggaaatt tttttgcagc tggttgtaat tttaatggaa cttggacagt aaaaggtcat
     2881 gctgaaaatg gattggctga taaaggttat agtggtaaaa ttgtagttga tggtttggct
     2941 acagagcttg caggacaaaa caatcaagct actgaagata gtaatggtgt ggatattcta
     3001 gtcagaaaag gatgcatgga aagagctatg ggtcaggcta gaggcggaag tttgataacg
     3061 tttggagcag gatataattc aggtctgtac atgtcagggg gagtattact aaatttggga
     3121 gcccctggaa aatcctttgg tcctggaatg gttggtggta ctatttattc tcctataggt
     3181 accacggttg gggaaggagc agcaattcga aatatgagta aaacagacta taaagttatt
     3241 ttttctgtgc taaaaaaatt tgaaaccgaa ttggaaataa aaggactaga taaattttcc
     3301 cccgaaaata atatattgaa cattacaaat aacggtaata gcgaagagta taattttaga
     3361 aattttataa aaatcaactc gactgaacta taacttgata gaatgttttg ttggtatatt
     3421 tgagttttct caagttgtaa ttgaataaat aaaaataaga atcctattct attggtaata
     3481 gattaaaaat taactctaac gtaaatacaa ttgagccaat attgggggat tttcattctt
     3541 ttatgttttg taaaattatt tgatagaaca acagtaaaaa aaaataatga aaattgtatt
     3601 agatattata aaaaagatag aaactatctt gaagtaaaaa agattagatt ttttaatccg
     3661 agtttaaaaa tcgaggagta tctcacgatt agatttcccc accattaggg agttattcgg
     3721 tccagttgac tttttattac ttgagaaaaa tacttgaatg taaattggta atactggaat
     3781 ctccttgaaa aacccctcgt acaaatatga aagaggaaca tggcatgttg gaattgattt
     3841 aagtgaaaag aaaaatgatt tatgttatag taaaatatgc acgaatttag ttccctacta
     3901 aagatagaaa gagagatcta tacataatga acttaatact aatatattca aaattatata
     3961 catttctatt gaacattgct tacaagaaaa aatactgtgt atttttgcta agtattctac
     4021 tattagtaag tattgatatc ttatcttaca atcacattgc gctaggcagt agcgatagta
     4081 atggtgataa cgatgataat gataatgata atgataataa taatgataat gataatgata
     4141 ataataatgg tggagaaaaa gaagaaaagg aaaaggataa tgatcacgat aacgatgatg
     4201 ttccatttat acttccagta ccatttcctt agggttgttt tgataatttt aaatactttt
     4261 gatattaaat tcctaccttg t
//
LOCUS       DASVEV010000099         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_34143, whole genome shotgun sequence.
ACCESSION   DASVEV010000099 DASVEV010000000
VERSION     DASVEV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34143"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            660..1190
                     /locus_tag="VF222_04700"
     CDS             660..1190
                     /locus_tag="VF222_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141758.1"
                     /translation="MISKQKCVCIACGRGFTRRTSGNRHNFNLHSGMSIIVESSLYYI
                     GISQGKYPTPDPTTAIRTRFCTPLNPNYPTHFNIDDKPIFNNFFKDNAAIIDSIIEKY
                     VDKLKPFLSEEEINKFVIDCIIRPITSTYINNKEELNRQVKMLNNVVGYIRIKRRLGD
                     KTALIFPDIKFEKMKI"
     gene            1695..2384
                     /locus_tag="VF222_04705"
     CDS             1695..2384
                     /locus_tag="VF222_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141759.1"
                     /translation="MFLLTNNNSDTSKKFFPYFSLLCIAASFFVMFIHIDLNQERNTV
                     ITRAWADDINGTENADNITGTINQDTIKGLKGNDTIYGKEAGDDISGGDENDTIYGNE
                     GRDWLRGGSGNDHIEGAKGNDMLFGDRGNDTLVGGPGNETITGGPNKDIFICGTATDT
                     ITDFNIRQKDTTPENDCENIIGITKNNDSLSQQNPNPEIPTIQEKMNSSVNTTNDKKS
                     PDEGLFFGLFK"
BASE COUNT          936 a          309 c          378 g          765 t
ORIGIN      
        1 agagtatttt atagaaatta acaatttagg aaactctgac atattagttg atggttatgc
       61 gtttactaaa ggaagtaatt tatcgttgat tggccaaata atgttaataa ttactggagt
      121 agtcatagct gttctaggta tgaggacaag acagtcataa atcaaaaaac ctcttgatat
      181 aattctatat ctcaaattgt agatatagta aatgaaagat tctatttgta tcaaacccgg
      241 tatatatgat agataagata gatgacgatt ttcttatgaa aaagaatacg attatggtct
      301 tttttgatta ctgtataaaa tagccctaga agttgaacgt caaattaatg caaattatgc
      361 aaatggagat aatttctcat ttacaattgt agatgaaggt tctaaaggta aattaggtaa
      421 tttgaatcct actaaaacag taacctaaat cctagtgatt attacttgga agagaacaat
      481 tttacattta ttgtaaatgt tggtactaca gacactgcgg aggctgtttt ctctatttca
      541 gcaaaggaat tatgagacca tcagaaagaa gtacactgta gagcactgta tagcactaac
      601 aacctgaact ttttttgtca tttttattaa gcttaacaac agtattgtta ttgtaactaa
      661 tgataagcaa acaaaaatgt gtatgcatag catgcggacg gggatttact agaaggacta
      721 gtggtaatag gcacaatttt aatttacatt ccggaatgtc tataatagtt gaatcttctt
      781 tatattacat tggcatatca caaggaaaat atcctactcc agatcctaca acagctatta
      841 gaacaagatt ttgtactcct ttaaatccaa attatcctac ccatttcaac atcgatgaca
      901 aaccaatatt taataatttc tttaaagata atgctgctat catagattcc ataatagaaa
      961 aatatgttga taaactaaaa ccatttctaa gtgaggaaga aataaataaa ttcgttatcg
     1021 attgtattat ccgtccaata acttctacct atattaataa taaagaggag ttaaatagac
     1081 aagttaaaat gcttaacaat gtagtgggat atatcaggat taagagaaga ttgggagata
     1141 aaactgcgct gatttttcct gatataaaat tcgaaaaaat gaaaatctaa tggaaaaaag
     1201 tttaaagtac aatccctacc aatgacgtca tcagttctta caacaaatag tgaaataatt
     1261 catttgtgat ccactaaacc attaaatgga agttgcaaat gagtaggata aagatatctc
     1321 atttcgacct aaggaataac attatattga ataaataatg agaaattgat ttatatattt
     1381 atttaggcac aatttagatt ttactttatc atccaaactt tccattcaaa acataccatt
     1441 taataggaaa taaatttcat tctcgaatat atctttttta gaattttaaa aactagtata
     1501 aattataaac ggttattata tgcatagtaa agaaagattg ctaatgcttc ataaatttaa
     1561 tactatatca attttatgga attatcttgc aatactcttt taaatcgatt ttttagtttt
     1621 tactatacta aaaaaagatg aaaattaaaa gaaaaattct taattgtaat actacgaaat
     1681 tttcgagtaa taaattgttt ttattaacca ataataattc agatacttca aaaaaattct
     1741 ttccttattt ttctttactt tgtatagctg cttcattttt tgttatgttt atacatattg
     1801 atctcaatca agaaagaaat actgttataa caagagcatg ggcggatgat attaatggaa
     1861 ctgaaaatgc agataacatt acgggtacaa taaatcaaga tactatcaaa ggattgaaag
     1921 gaaatgatac aatatatggc aaagaagcag gagacgacat tagtggtggt gatgaaaatg
     1981 atacaattta cggaaatgaa ggaagagatt ggctacgagg tggaagtggg aatgaccata
     2041 tagaaggagc aaaaggaaat gatatgttat ttggtgatag aggaaacgat acactagtag
     2101 gaggtccagg caacgaaacc attactggtg gtcccaataa agatatattc atctgtggaa
     2161 cagctacaga cacaataaca gattttaaca ttagacaaaa agatacaact ccagaaaatg
     2221 attgtgaaaa tatcattggt attacaaaaa ataatgactc tttatctcaa caaaatccta
     2281 atccggaaat tcctactatt caagaaaaaa tgaatagtag tgtaaataca acaaatgata
     2341 agaaaagtcc agatgaagga ttattttttg ggttatttaa ataggtaa
//
LOCUS       DASVEV010000100        10981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_423952, whole genome shotgun sequence.
ACCESSION   DASVEV010000100 DASVEV010000000
VERSION     DASVEV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423952"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VF222_04710"
     CDS             <1..1176
                     /locus_tag="VF222_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7141760.1"
                     /translation="TTAFAISFLSGISVIAQNNNNAFSKEEEKTTTRETVVTGGHTTG
                     YNTIRTTGDVLDPINADERTDLTIDPMKYLREFNYGKVSTTDNGTTLREFTIIAQDRN
                     LEVSPGVFMDAWTFNGTIPGPTIRATEGDLVKIKFINNGSKPHTMHFHGIHQSHMDGV
                     FEIIAPSGSFTYEFTAEPFGLFLYHCHMQPLEEHISKGLYGVYIVDPKTPRPQADEMV
                     MMMNGYDNDFDTENNFYTVNGIPNYYMHHPIQIEKDKLIRVYLVNILEFDQINNFHLH
                     ANLFQFYRTGTSLTPNEYTDIVTFSQGERGILEFSYKYPGPYMFHAHKTEFAEKGWTG
                     LFLVKEDPSESDLTAFTKGEKAVYPAEVSNNTFTTQTKTKDSQSTVVSNRESSQGGS"
     gene            1182..2537
                     /locus_tag="VF222_04715"
     CDS             1182..2537
                     /locus_tag="VF222_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7141761.1"
                     /translation="MESNENKIDRNNRKTSRKKMIVIALIPLIVLSGMLVFLLGPGMS
                     ILNAGIPLPEITIEKIEFQKDKIVAIIRNTGPEGVEIAQADINDRMIPAAIEPSKTLS
                     RLAEAKVIVPFSWIAGVPYEVGVTSSDGTRFSKNVEAAALAPTPNVEQASYFALIGIY
                     VGVIPVLIGLLWFPFIKRLGMNKYKFFLSLTAGLLVFLGIDALVESNEIVAQNIAGAF
                     NGQVLIAMFTIITFLVLLYTSEKLVERAKIKSDTNKSKSNLVTYSSSSSSTTTEGEQK
                     HQQQQQQKHQQQQQQQQQLAKPFAIAMMIAIGIGLHNFGEGLAIGAAVLLGEVALSTF
                     LIVGFTLHNTTEGLAIVAPMAKSGSKVTLRKLITMGLIAGVPTIIGAWIGGFLYSPIA
                     AIIFLSIGAGAIFQVVFSITSWMSSNTSEPRKAMLSTPMIAGFAAGMAIMYVTSLLIP
                     S"
     gene            2583..3056
                     /gene="mntR"
                     /locus_tag="VF222_04720"
     CDS             2583..3056
                     /gene="mntR"
                     /locus_tag="VF222_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HEX7141762.1"
                     /translation="MLRKLKGDSNSGINRLESIRDAHTGKKENEDRTDRMEDYLEVIY
                     ELVQQKGYATTIDISQFLNVSSPSVTKMVKRLDETGYLKYEKYRGICLTQQGIDVAMN
                     IHQRHSLLAEFLKIIGVDEDIANRDAEGIEHHLHPQTLKKLEEFVMIVKKNHDDF"
     gene            3949..4230
                     /locus_tag="VF222_04725"
     CDS             3949..4230
                     /locus_tag="VF222_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141763.1"
                     /translation="MSFEGISFGKLALIFIVAGVVLVLAIMGSSATNLFRQSVTEDAT
                     VEIKHEGNCVVEASDKIPRTIVNCPYNVNDTLVITYKPQRPTIENYESK"
     gene            complement(4332..4802)
                     /locus_tag="VF222_04730"
     CDS             complement(4332..4802)
                     /locus_tag="VF222_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX7141764.1"
                     /translation="MYLCCYVSFVFVLLDLSLRNTSKALVIFRDEKKRNYVSVWNRIQ
                     RSASYQIYRRTRVSAFIIDETVIQIGSQHFWLWLCIEPVHRFMLGIYSSEEKNMLVAE
                     KFIRSLVDKYGRDPGSTKGGTWYPEAFNVIGLKHKQSCLISSCVISDTILLFIR"
     gene            5382..6269
                     /locus_tag="VF222_04735"
     CDS             5382..6269
                     /locus_tag="VF222_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141765.1"
                     /translation="MIISKYIKKISITILLTSFAIICGGVGSLVFENSFAHNFSPDES
                     AHFLTLVDKIKVESSLVANSSGNNNQSSAIEHAEYALNNYDTHTNDEISERNERVANE
                     LNSTLHQLLDQVQSSNDKSQISQSVETINAILEEATSVRIDQEQLNNSTIQALVFANI
                     VNSALQSYGTAFNIGIDLTNMSNLHTKSMENNTLHKEGNNHEFQNVSHKTIIENFSSY
                     ESAISFSDYTLKKFKNEIKTSVTENNTTTQNYLKKLEDGLLELNTVIKNKDNPMKAME
                     IVHTKIHPNLQILFNLKSK"
     gene            6670..7062
                     /locus_tag="VF222_04740"
     CDS             6670..7062
                     /locus_tag="VF222_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141766.1"
                     /translation="MKNNKFSSLILLVVLSFLNVLCYQAVDANELIVDIEPKCGKLKD
                     HRINMTVNGFNKNGNVYWEFIKSNGYMDYYGYFYTNATGGFNDYTIADGLLPDNYTLR
                     FFDDKNNDYVKDKNGLEMNLTYIIPCIK"
     gene            7453..8121
                     /locus_tag="VF222_04745"
     CDS             7453..8121
                     /locus_tag="VF222_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141767.1"
                     /translation="MYSDSNLYSVEAKQSTISDHEFHGYLEQMIGHLDASILNVHNNN
                     SVLAVAHALHPIEEIVDVINSKLIQTNETLTKDFSSKLNKYVQTVRSDNLNKIIIEKR
                     ELEKLLNDALDQSISLEKRNNRDYILNVTAELITIASEEYTESMNQGRIINLLEFQDG
                     QQFFNRAITLLNKSQASLSDNDKNYLTGLNELEKGMDSKIDPSEIDMNVKDMLCEIKK
                     GLYC"
     gene            complement(8512..8844)
                     /locus_tag="VF222_04750"
     CDS             complement(8512..8844)
                     /locus_tag="VF222_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141768.1"
                     /translation="MAVKFANKFQNDILDKLVFMLDLSVIGNEHVKRGLLFSAVNTSH
                     DVAKKNRSNILLIGDPGVGKSKLLKRTSKVVPNRRYESGENSSGKNLNGIIEKFDDNT
                     QILGIFSR"
     gene            complement(8853..8993)
                     /locus_tag="VF222_04755"
     CDS             complement(8853..8993)
                     /locus_tag="VF222_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141769.1"
                     /translation="MFLSRIDKEDNNNLDNNILNSYIVVNPNEIKNTTKMEQEGNQLF
                     II"
     gene            complement(9282..9527)
                     /locus_tag="VF222_04760"
     CDS             complement(9282..9527)
                     /locus_tag="VF222_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141770.1"
                     /translation="MIKGQIQSEVIEDVQAELDKSGDSTIKRTFTFDRDTDVGKIQIG
                     DRYHACVASEDLRPPEGSECEKRVVKHFDKPNSLPAR"
     gene            complement(9626..9748)
                     /locus_tag="VF222_04765"
     CDS             complement(9626..9748)
                     /locus_tag="VF222_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141771.1"
                     /translation="MTKFDCNEGDLLNIKKPTEINLFTTGSQVYAKSQAVYEKI"
     gene            complement(9883..10146)
                     /locus_tag="VF222_04770"
     CDS             complement(9883..10146)
                     /locus_tag="VF222_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141772.1"
                     /translation="MIKRGYLRYIYIFIAISFAVNILSIYSPNESVFAKKDKDADNEK
                     DKDQLVVNARINLNNIDMKNTEFIWVTAFINGEDFNKIFLFHL"
     gene            complement(10612..>10981)
                     /locus_tag="VF222_04775"
     CDS             complement(10612..>10981)
                     /locus_tag="VF222_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7141773.1"
                     /translation="GMAWFKQMQNKVWFQLSVTDMQGVTQAHIHTGKVGENGPVVVTL
                     YKSDTPQPINGKLSYGNITANVLEGPMKGKQLSDLATAMSNGSTYVNVHTEKYPNGEI
                     RGQIMMANSTSDAIMMRTMK"
BASE COUNT         3925 a         1610 c         1657 g         3789 t
ORIGIN      
        1 actacagctt ttgctatttc ttttcttagt ggaatttcag tcattgcaca gaataataat
       61 aatgcctttt caaaggagga agaaaaaaca acaacaaggg aaacagtagt gactggcgga
      121 catactactg gatacaatac cattagaact acaggtgatg ttttagatcc aatcaatgca
      181 gatgaaagga ctgatctgac tatcgatcca atgaaatatc taagagagtt caattacggt
      241 aaagtatcga caacagacaa tggaaccaca ttacgcgaat ttactataat agctcaagac
      301 aggaatcttg aagtatcacc aggtgtattt atggatgcat ggacatttaa cggaacaatt
      361 ccaggtccaa caattagagc aacagagggt gatctagtta aaatcaagtt cataaataat
      421 ggatcaaaac ctcatactat gcatttccat ggcattcatc aatctcatat ggatggagta
      481 tttgaaatta ttgctccaag tggaagcttc acttatgaat ttacagcgga accatttggt
      541 ctttttcttt accactgtca tatgcagcct ttagaagagc acatatctaa gggtctttat
      601 ggtgtttata ttgttgatcc aaaaacgcca agaccacaag cagatgaaat ggttatgatg
      661 atgaatggat atgataatga ctttgatact gaaaataact tttacactgt aaacggaatt
      721 ccaaattatt acatgcacca tcctatccaa attgagaagg acaaattgat acgagtatat
      781 ttggttaata tattagaatt tgatcaaata aacaattttc atttgcatgc aaatctattt
      841 cagttctata gaactggaac cagcttgacg ccaaatgagt atacagatat agtgacattt
      901 agtcagggtg aaagaggaat actagaattc tcctacaagt atccaggtcc ctatatgttc
      961 cacgctcaca agacagaatt tgcagagaaa ggatggactg gcttgttctt ggttaaagaa
     1021 gacccgtctg agtcagattt gactgcattt actaaaggtg aaaaagctgt atatccagct
     1081 gaggtaagta acaatacttt tactacgcaa actaaaacaa aagattctca atctacagtt
     1141 gttagtaata gagaatcttc acaaggaggt agttaataca aatggaatct aatgagaata
     1201 aaatcgatag aaataacagg aagacgagtc gaaaaaaaat gatcgttatc gcattaattc
     1261 cattaatagt actgtctggc atgcttgtat ttcttcttgg tcctggtatg tcgatattaa
     1321 atgcaggaat acctttgcct gaaatcacaa tagaaaagat agaattccaa aaagataaaa
     1381 tcgtagcaat tatccgtaat actggtccag aaggagtaga aattgcacaa gcagacatta
     1441 atgatcgtat gattccagct gcaatagaac caagtaaaac cttgtcaaga ctggccgaag
     1501 caaaggttat agtacctttt tcgtggatcg ccggcgtacc atatgaagtt ggagtaacta
     1561 gttctgatgg tacacggttt agcaagaatg ttgaagcggc tgcattagca ccaactccaa
     1621 atgtagaaca agcatcatac tttgcactta ttggtattta tgtaggagtt atacccgtgt
     1681 tgataggtct tctctggttt ccatttataa aaagacttgg catgaacaag tacaaattct
     1741 tccttagtct tactgcagga ttgcttgtct tcttgggaat agatgcacta gtagaaagca
     1801 acgaaattgt agcacaaaat attgcaggag cttttaatgg tcaagtattg attgctatgt
     1861 tcacaatcat tactttcttg gtattgctat atacatctga aaaacttgtt gaacgagcca
     1921 aaattaaatc tgataccaat aagtccaaat ctaacttggt aacatattct tcttcttcct
     1981 cttctactac aacagagggt gagcaaaaac atcaacaaca acaacagcaa aaacatcaac
     2041 aacaacagca acaacaacaa caattagcaa aacctttcgc tatagcaatg atgattgcaa
     2101 taggaattgg attacataac tttggagaag gtttggcaat aggtgctgct gttctattag
     2161 gagaagtagc cctcagtact tttctaatag ttggttttac attacacaac actactgaag
     2221 gattggcaat agtagcacca atggccaaaa gcggtagcaa agtcacatta cgaaaactga
     2281 taacaatggg tttgatagct ggtgttccaa caataatagg agcatggatc ggagggtttc
     2341 tttattcccc tattgctgca ataatattct tgtcgatagg tgctggtgca atatttcaag
     2401 tagtattttc catcacttcc tggatgtcat caaatactag tgaaccaaga aaggctatgc
     2461 taagtacccc tatgattgca ggatttgcgg caggaatggc aataatgtat gtaacatcat
     2521 tgctgatacc atcttaacta attgttagta actaaaatat acataaggat ggtaaataaa
     2581 aattgttaag aaagttgaaa ggtgactcta attcaggtat caatcgtctt gaatcgattc
     2641 gtgatgctca taccggaaag aaggaaaacg aagaccgtac tgatagaatg gaagattacc
     2701 ttgaagttat ttatgaattg gtacagcaga aaggttatgc tacaaccata gatatttctc
     2761 aatttcttaa tgtaagttct ccaagtgtaa ctaaaatggt aaagcgattg gatgaaactg
     2821 gctatctcaa atatgaaaaa tataggggga tttgtctaac ccagcaaggg attgatgtgg
     2881 ccatgaatat tcatcagaga catagtttgt tggctgaatt tttaaaaata attggagtag
     2941 atgaagatat tgcaaataga gatgctgagg gtatagaaca tcatttacat cctcaaactt
     3001 tgaaaaagtt agaagaattt gtaatgattg taaaaaagaa tcacgatgat ttttaaatat
     3061 tgcatccatc ttcctattaa taggattatt aacattatta atttatacac atgtgatata
     3121 gtcatttaat ttattttaga gtcctaaaaa tgggtgttag gattaacttt aacacccatg
     3181 taattgaagc tcaagccaag cttaagtaaa taccatacaa aaaataattt aaaatacatt
     3241 gtgtttaatt attagagaat tattttagtg acgaagaccc aacatttttt acaagcttat
     3301 ctaatctaca gagaatgttt acaaaaccat gtaattaagt tttaagcata tgttaatctc
     3361 aattttagaa atggactttt cctcttattc atttccgata ttatttgtat taactgtata
     3421 acatttcata tcgggaaaat gtatcatcgt ataacattat tgaaagccat ataaagcgtt
     3481 aataataaaa taaaactgcg atcccatgat tagtatcctc aattcgaaac atattttgta
     3541 acttcattta caaccttaac attaactaag aaataagttt acttttgata gtctaatctt
     3601 atactttatt ccaacattgt attttactaa tagaaaaata gcacaactat taaaatgtta
     3661 aaatacaatc gagtaatgta attgaaattt cgtaattata atgtaataga attaatgggc
     3721 attataatat taatctctcc tatactacac aattctaatt ttcgtggaga agataatagt
     3781 aatagaggat acacttttat caatgaaaat tttttacaaa attcttaaag attctaatgc
     3841 ctcagaggtt gtattaaatt atcaaattcc ttggaataat tcagaataat taagtcaatc
     3901 cttatcaata aatatattaa attaaattgc tattaaatta tcataataat gagtttcgaa
     3961 ggtatttcat ttggtaaatt agcacttata ttcattgttg caggagttgt attagtcctg
     4021 gcaattatgg gttcatctgc aaccaatctt tttcgtcaaa gcgttacaga agatgcaaca
     4081 gtagaaatta aacatgaagg aaattgtgtt gttgaagcat ctgataagat accaagaaca
     4141 atagtaaatt gtccctataa tgtcaatgat accttggtaa ttacctataa accacaacga
     4201 ccaaccatag agaattatga gtcaaaataa aagataatta attgatctca ttttctttgt
     4261 atatgaaatt taaaaaataa tatttataaa aaatagtttt acagataaaa attgtttgaa
     4321 attagaaaaa attatctgat aaagagcaaa attgtatcag agatcacaca agaagaaatt
     4381 agacaagatt gtttatgttt taatccaatt acattgaatg cttctggata ccatgtacca
     4441 cctttagtag aacctggatc tcttccatat ttatcaacaa gagatctaat gaatttttca
     4501 gcaactagca tatttttctc ttccgaactg tagattccaa gcatgaaacg gtgtactggt
     4561 tctatacaaa gccataacca gaaatgctga ctgccaatct ggataacggt ttcatctatt
     4621 ataaaggcag atactcttgt gcgtcggtag atttgatatg aagcagatct ttgaatccta
     4681 ttccaaacag atacataatt tcttttcttc tcatctctaa atatcactaa tgctttagat
     4741 gtattacgta aacttaaatc tagaagtaca aatacaaagg aaacataaca acaaaggtac
     4801 atgttctatt tctttcaaat atcctaatga ccaatagatt ggaataggac atttatagct
     4861 aaaatctatt tctctaaatt aacatagccc taaaagatat gcgacgaacc atcaagagca
     4921 taattatatt tgattatctt ctccaaaaat gtctcttgta agacattcaa atcagtatat
     4981 atattagaat tacttttgtt ttaaaaaaat aaagatgagt taagtgaagg caagattgta
     5041 tttacattta ttcttcttca tatgatgcat attcgtttcc gtatttgttg tcattatcat
     5101 aagtgtattc atatccgtat tgctcatcat atttgttgtt gtcgtactcc ttatactcct
     5161 catctttttt atcattcttg attaaattct atttgagaat aattatttat ctaatataat
     5221 taaatgaata aattacttta ctatttgttg tttttgtctt ggtctttctg acatatttta
     5281 taagtacatt atctcttgta ttatttttaa catttttata acattatctt tattttagat
     5341 gccaaaattc ttattatatt aaaatacaat aataatttaa aatgatcatt agcaaatata
     5401 ttaagaagat ttctataacc atattactca catcttttgc aataatttgt ggtggtgttg
     5461 gctctcttgt ctttgaaaat tctttcgctc ataatttctc tccggatgag agtgcacatt
     5521 ttctaacact tgttgataaa ataaaagttg aatcatcact tgtagcaaat agtagtggga
     5581 ataacaacca aagttcagct atagaacatg ccgaatatgc tctcaataat tacgatactc
     5641 atactaacga tgaaatttca gaaagaaatg agagagttgc aaatgaatta aatagtacat
     5701 tacatcaatt actagaccaa gtccagtcat ctaatgacaa atcacaaatt agtcaatcag
     5761 tggagacaat taatgcaatt ttagaggagg ctacatctgt aagaattgat caggagcaat
     5821 tgaataattc tactattcaa gctctggtat ttgcaaatat tgtaaactcc gctcttcaaa
     5881 gttatggtac tgcatttaac atcggaattg atttaacaaa tatgtcaaat ctgcatacaa
     5941 aatcgatgga aaataatact ttgcataaag aaggtaataa ccatgaattc caaaatgtgt
     6001 ctcataaaac gattattgaa aatttctcga gttatgaaag tgcaataagt ttttctgatt
     6061 atactttaaa aaagttcaag aatgaaataa aaacatcagt tacagaaaat aatactacaa
     6121 ctcaaaatta ccttaaaaaa cttgaagatg gattattgga attaaatact gtaattaaaa
     6181 ataaggacaa tccaatgaaa gcaatggaga ttgttcatac taaaatacat cctaacttgc
     6241 agatcctttt caacttaaaa tcaaaataat attttttaat ttaattatag gtaaatcttt
     6301 attattgtga ataatgaaat tagaacacat attattataa tccaagacca aattcttatg
     6361 atttggaacg gaaatattga tttatacaaa atatcttcct ctatcaatct tatagcatat
     6421 tcagaggatg atgattatgg tttagataac aattttatta catgccaaat attattatgg
     6481 agaagaaaaa agataatcat tttaagtata cagcaataga tattctattg atatataaca
     6541 tataggctgt caaaagcaac tttaacacct atcattcaaa acaaagttat atttacaata
     6601 taaatcaaac taagatgatt ctatttagta tatatattta aattaagatt caatatcaaa
     6661 agaagtacag tgaaaaataa taaattttca tcattaattc tattagttgt tttatcattt
     6721 ttaaatgttt tatgttacca agcagtagat gctaatgaat taattgtaga tatagaacca
     6781 aagtgtggaa aattaaaaga tcatagaatt aacatgactg taaatggttt taacaaaaac
     6841 ggtaatgtat attgggaatt tataaaatct aatggttaca tggattatta tggatatttt
     6901 tatactaatg ctacaggagg atttaatgat tatactatag cagatggtct tttaccagat
     6961 aattatacac ttcgtttctt tgatgataaa aacaatgatt atgttaaaga taaaaatggg
     7021 cttgaaatga atctaacata tattattccc tgtattaaat aatctagaca ttctatccaa
     7081 gtagatgtta ataaataata cggatcgtaa tattttcggg gaaatgttaa aaatgtatct
     7141 aataaataaa tagttatttt ttttcttatt gttttttact cattcaaaat gtataggcca
     7201 attacagcaa caaccggagt tatcaaatac tatgatcaaa gtaatttcat aaacgaattg
     7261 gaagtacctg ttgacaataa caatagaggc ttaaagggaa taatcattaa tttggaagat
     7321 aatgtttggt tttatcatat tagaattatg catagactaa aatatacatc ttaattttga
     7381 atatacacaa tgattttgaa ttttagctta acaatatttg taatcccaat atgctttacc
     7441 attttttcct tattgtattc cgatagcaat ttatattctg tcgaggcgaa acaatccacc
     7501 ataagcgatc atgaatttca tggttatcta gaacaaatga taggacattt agatgcttca
     7561 attctaaatg ttcataataa taatagtgta ttagctgttg ctcatgcatt acatcctatc
     7621 gaagaaattg tagatgtaat aaacagtaaa cttattcaga caaatgaaac attaacaaaa
     7681 gatttctcaa gcaaactaaa caagtatgtt caaactgtaa gatccgataa tctaaataaa
     7741 attattatag agaaaagaga attagaaaaa ttacttaatg atgcattaga tcaatctata
     7801 tctttagaaa aaagaaataa tcgagattat atactcaatg ttactgcaga attgataact
     7861 attgcatctg aggaatatac agaatcaatg aatcagggaa gaatcattaa tttattagaa
     7921 tttcaggatg gtcaacagtt ttttaacaga gctattactc ttttaaataa atcacaagcg
     7981 tccttatcag acaatgataa gaattatctt acaggcttaa acgagctgga aaaaggtatg
     8041 gattccaaaa tagatccaag tgaaatagat atgaatgtga aagacatgct ttgtgagata
     8101 aaaaaaggac tttattgtta gttaagttaa ttatttgagg attgatggca attcttttga
     8161 taggtaacat atagaggtat tgttttgagt atgatgtctt agtgacacaa ttatattatc
     8221 ggataggcta ttgatccgta attaatgatc tattctagta ggattttgga tttgataatt
     8281 tgatttcatg gtccaaatca aaatatgaaa tatggtataa tacatatgtt attatgcttt
     8341 tattattcta atataatttt tagtaagaat ctggtttgat tagaccaaaa taaagcaaaa
     8401 ttttgtatgt gtattctacg atgattttca tataatagac gaaataaaga aagcataggg
     8461 agtgtcgaga tagtaacagg tagaaatttt tgacttaaag ttgattaata tttatctcga
     8521 aaatattcca agtatttgag tattatcatc gaatttttca ataataccgt ttagattttt
     8581 accagatgaa ttttctccac tttcatatct tctatttgga actacttttg aagttctttt
     8641 caatagcttt gatttgccaa ctccagggtc accaattaat aatatattac ttctattctt
     8701 ttttgcaaca tcatgtgatg tattaacagc agaaaataat agtcctcttt ttacatgctc
     8761 gttccctatc actgaaagat caagcatgaa gacaagtttg tctagaatgt cattttgaaa
     8821 tttatttgca aattttacag ctatttaatg atttaaataa taaataattg atttccttct
     8881 tgttccattt tagtggtatt ttttatctca ttaggattca caacaatata tgaatttaaa
     8941 atattattgt ctaggttatt attatcctcc ttgtctattc tacttaaaaa catcttcact
     9001 ctataatttg tttttataag tcttcttatt ataaaaatat ttacattaac aataaatttg
     9061 tattcttgct gttcaactgg ggatgacccg aattgattaa ttgttaattc aatacaaatt
     9121 tttttgtgaa ttgtgttttt ccctatgttt tgtaatctgg ataaaagatt gcagatgttg
     9181 tccttatgag ggacaattta tttggagttg tctattctct taaattagat agatgaaatt
     9241 atatgaaaaa atgagtaatt aatatttcaa aaaggtagta cctagcgagc aggcaaggaa
     9301 ttaggcttgt caaaatgttt aactactctt ttttcacatt cagaaccctc tggtggtctt
     9361 aggtcctcac tagcaacaca tgcatgatat ctatctccaa tttgtatttt gcctacatca
     9421 gtgtctctat caaaggtgaa tgttctcttt attgtactat ctccactttt gtcaagttct
     9481 gcttgaacat cttctataac ttcagattgt atttgaccct ttatcatggc acccattacc
     9541 aactttttag tatctctttt atctgccaga ggagaaagaa attttatttt tactctatcg
     9601 ccatttgagc caattacatt actattcaga ttttctcata aactgcttgg ctttttgcat
     9661 atacttgtga accagttgta aacaaattaa tttctgttgg tttttttata tttagtaaat
     9721 ctccttcatt acaatcaaat ttagtcaaag aattatcgtc ttttaattca ccgtcaactt
     9781 gatatgcaca tataaaaaat tcatttgggg tatctgcctc aactatatca tcatttctaa
     9841 catctgtttt taattctaca tctaattttt tcttactttt gtctatagat gaaataggaa
     9901 tatcttgtta aagtcttcac cgtttataaa tgccgtaacc catatgaatt cagtattttt
     9961 catatctata ttgtttaaat taatacgggc attaactact agttggtcct tatctttttc
    10021 attatcagca tctttatctt ttttagcaaa aactgattca ttaggagaat atatggatag
    10081 gatattaaca gcaaacgata ttgcaataaa aatatatatg tatctaaggt aacctctttt
    10141 tatcatctta atttgttact aactatccag tatatttaat attttcgtat gaaaattcta
    10201 taatgtaata cagtataaaa ttatgaattt gttttaaaaa aaattgctaa tacatccata
    10261 actattattt tgtccatact atatcaatat cattatcaaa agatggttaa tgagtatgat
    10321 agtgttactt ttacaatcat tgatgatgaa taattgtcat agccttaact tgagatttac
    10381 aatgccggtc caggctatga agatatatta agtagttttc ctatgacgtg taatcaatat
    10441 ctaatgatag caacagtatt tgtcggtgag aaaaaaaaga ttatcttaga gattggaaaa
    10501 gtaaataata acttactatt agttgttgtt aaaaatcaga taataaattg gttacaactg
    10561 attcatttaa acataatttt cttagttatt aaaataaaaa atagaaaaga attatttcat
    10621 cgtcctcatc atgattgcat cactagttga atttgccatc attatttgtc cacgaatttc
    10681 accgttagga tatttctcag tatggacatt tacatatgta cttccgtttg acattgcagt
    10741 agctaggtca gatagttgtt tacccttcat tggtccttct aacacatttg cagttatgtt
    10801 tccgtatgat aacttaccat ttattggttg tggagtgtcc gatttgtata atgttacaac
    10861 aacaggacca ttctcaccta cttttccagt atgtatgtga gcctgtgtga ctccttgcat
    10921 atcagttaca ctaagttgaa accatacctt attttgcatt tgtttaaacc atgccatacc
    10981 a
//
LOCUS       DASVEV010000101         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_34562, whole genome shotgun sequence.
ACCESSION   DASVEV010000101 DASVEV010000000
VERSION     DASVEV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34562"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF222_04780"
     CDS             complement(<1..291)
                     /locus_tag="VF222_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141774.1"
                     /translation="MSSSSIIKKTSTATGFIKAIESGSKHLLMKRFTLRFPEQKLKFV
                     GDGYQFDPKRGVGIAGSRGRHILFHEKCTGCQLCAITCEGIAEAIEMVKVPEK"
     gene            complement(296..445)
                     /locus_tag="VF222_04785"
     CDS             complement(296..445)
                     /locus_tag="VF222_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141775.1"
                     /translation="MKEREFTSATKSIITFLANYIMNMNHIFIVPINLTGIIDKKGTL
                     GGGLQ"
     gene            complement(827..2005)
                     /gene="dinB"
                     /locus_tag="VF222_04790"
     CDS             complement(827..2005)
                     /gene="dinB"
                     /locus_tag="VF222_04790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7141776.1"
                     /translation="MDDKKQWRGKAILHVDINSFYSSCEEIKDPSLIGKPHAVIMTPQ
                     DNNSITKGAVTTCSYEAKKLGIKSAMPLYKALELCPNLILHSVDKKFYEKMSNEVMKI
                     LEDYADIFEQASIDEAYLDCTIKISNINITVEEYAQEIKKSIKEKCSGLLTSIGIAPT
                     KSVAKIASDYQKPDGLTIVNFNELKNFLSPLDVETISGIGIKTQKILKEQMNIKTIGQ
                     LSNTNVQLLIERFGKKIGTWMWQVANGLDNEPVLPRGNHISLSNESTLEFVTEDKEII
                     KRSLYELIDELYERIKNNKYQFRTVGIKLMRTDFSIETREKSYIKYQSERKSIESIIE
                     EILGKFNLEDDNLVIPSKKITKKFLPIRKVGLKVSNLTTREKNSKDKEYKQKTLLDYC
                     "
BASE COUNT          655 a          370 c          299 g          892 t
ORIGIN      
        1 tttttcagga acctttacca tttctatagc ttcagcaata ccttcacatg ttattgcaca
       61 gagttgacat ccagtacatt tctcatggaa gagaatatgt cgtcctctgc tccctgcgat
      121 tcccactcct ctctttgggt cgaactggta tccatctcca acaaacttca atttttgctc
      181 tggaaatcgt aacgtaaatc ttttcataag aagatgtttc gaacctgatt ctatagcttt
      241 aataaatcca gttgctgttg acgttttttt aataatagat gatgatgaca ttttattact
      301 gtaaacctcc tcccaaagta ccctttttat cgataattcc tgttaaatta ataggcacaa
      361 tgaaaatatg attcatattc attatataat tagccaaaaa cgttattata cttttagtag
      421 cgcttgtaaa ttccctttct ttcattctat cgtaaaatat ttgtcagcgt catgataaac
      481 gtcgtgttcg ttaaggggga tacggccatg tctgtatcat gtatatatcc tgtttcttgt
      541 ccagtgttct ctggaccatc tttatctgat agtcgggatt actgttctaa ggtgaaccct
      601 actaatgaca tttactatta ggatttataa tgaatagtaa gagtaagaat aatgattctc
      661 taattatttg acctttatac tctacaaaca aagtggttga ctcatatcaa aactgcatat
      721 tcaagacttg aaccttgggc tatggtttta ttatcaaaat gaaaaatttc taaatataac
      781 aattaattta tgcacattta tagaaagctt agtttgattg taatggctaa caataatcta
      841 ataatgtctt ttgtttgtat tctttgtctt tactattttt ttctcttgtt gtcaaatttg
      901 atactttcaa tcctactttc cttatgggta aaaatttttt agtaattttt ttcgaaggaa
      961 tgaccaaatt atcgtcttcc aaattaaatt tacctaagat ctcttctatt atggattcaa
     1021 tactttttct ttcgctttga tatttgatat aagatttttc tctagtttca atactaaaat
     1081 cagttctcat taattttatt ccaactgttc taaattgata tttgttgttc tttatccttt
     1141 cataaagttc atcaatcaac tcatacagag atcgctttat tatctcttta tcctctgtaa
     1201 caaattctaa tgtagattca ttgcttaatg atatgtgatt tccgcgtgga agaactggct
     1261 cattgtctaa accatttgca acttgccaca tccatgttcc tatcttcttt ccaaatcttt
     1321 ctatgaggag ttgaacattt gtatttgata gttgacctat agttttgata ttcatttgtt
     1381 cttttagaat tttttgtgtt ttgattccta ttcctgaaat tgtttcaaca tctaatggac
     1441 taagaaaatt tttcaattca ttaaagttta ctatagtaag accgtcaggt ttttgataat
     1501 cagatgcaat ttttgcaaca gattttgttg gtgctatacc tatagatgtt aataagccac
     1561 tacatttttc ttttatagat tttttaattt cttgagcata ttcttccact gttatattaa
     1621 tatttgaaat tttaattgta cagtctaaat acgcttcatc tatacttgct tgttcaaaaa
     1681 tatctgcata atcttctaat attttcataa cctcattaga catcttttca tagaattttt
     1741 tgtctactga atgtaatatt aaattaggac ataattctaa tgctttgtat aacggcattg
     1801 ctgattttat tccaagtttt ttagcctcat aagaacatgt tgttactgca ccttttgtaa
     1861 tgctgttatt gtcctgaggt gtcattataa ctgcatgagg ttttcctatt aatgatgggt
     1921 ctttaatctc ttcacaagat gagtaaaatg aattaatatc gacatgcaaa attgctttac
     1981 ctctccactg ttttttatca tccatatcca taccctgtta ttactcaaat gattggtatt
     2041 agattaggat taaactttcg tttatgtata aaaaaaatct ttctatgata ggcttaataa
     2101 atgtaaattt gtaatattat attgggaatt ataattagat aattacccag aagaaacagc
     2161 ttttaaacct atttagtata caaacaatgc ctaccagggt catgttggac cacagg
//
LOCUS       DASVEV010000102         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1200080, whole genome shotgun sequence.
ACCESSION   DASVEV010000102 DASVEV010000000
VERSION     DASVEV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200080"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF222_04795"
     CDS             <1..229
                     /locus_tag="VF222_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX7141777.1"
                     /translation="MSPKMIGVTVEDGAIIAARAVIKPGVTIGKNAVVGMGAVVTKDV
                     PPETVVIGNPARIKYSRKEYDQKKAEWEQTV"
     gene            complement(848..1411)
                     /locus_tag="VF222_04800"
     CDS             complement(848..1411)
                     /locus_tag="VF222_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141778.1"
                     /translation="MSLILGSGLAAFTSNSASAQNYGYQDENSYNSYSDSSSSSYGDT
                     YSKYPTEDKNHECRTGPFEGFFTSSVEFCKDGKFDDRKGHDRDRDNRTVTIDTYTVVG
                     NTSVGFAGTNSSVISSTALCDVGDPATGGSFVTAGNSTIRASQALANETGWIATAEVF
                     GNATIPGAPLGSVTSDVECLDNPPLRP"
BASE COUNT          688 a          298 c          324 g          735 t
ORIGIN      
        1 catgagtcca aaaatgatag gagtgactgt agaggatgga gcaataatcg ctgctagagc
       61 agtaataaaa cctggggtta ctattggaaa aaatgctgta gttggtatgg gtgctgttgt
      121 aacaaaagat gtgccacctg aaacagtcgt tattggcaat cctgctagga ttaaatattc
      181 cagaaaggaa tatgatcaaa agaaagcaga atgggaacaa acggtttaga tatatataaa
      241 gaagttaagg tcaaaattgg tcacaatagt caaactattc gtagcccatt ttttttataa
      301 aaaaaataga aataataata ctatagaagc aggcagcaag gagatattcc ttgacttaat
      361 gatattggaa ttacttggag gagaagtcca cctaatataa tttagattgt acaatcatat
      421 tcagtcatat tgaactagga tatctcttcc ttaattattt agttataata gatattttat
      481 gttcattgca ctccataact tcaaaagaat ttatcacttg ttttaagact acctgttctt
      541 gcatacctac ttttaatttt aatattctca ctttatttaa gagctttgat agctaacttt
      601 attaaattct catattggga gaacagcagg tttacaacag ttaaaattta tttgataatg
      661 agaatcttgc aaagattgtt ccttatgtta agacaaaact aattcgtaaa aatatgttac
      721 ttcgactata taaattctaa aaaactattc gattcaatat taaaagcagg aaggattaat
      781 tttaatgatt atcaattgac tagtacttgt tttattataa tttaaaaaaa aataaaaaag
      841 ggggttttta aggcctcaat ggaggattat ctagacattc tacatctgat gttacgctac
      901 ctagcggtgc tcctggtatt gttgcattac caaatacttc tgcagttgca atccatccag
      961 tctcgtttgc caaagcctga gatgctcgta tggtagagtt accagcagtt acaaatgatc
     1021 cgcctgttgc aggatctcct acatcacaaa gagcagtaga gctaatgact gaactgtttg
     1081 taccagcgaa gccaacagaa gtattaccta caactgtgta tgtatctatt gttactgttc
     1141 tattatctct atctctatca tgaccttttc tatcatcgaa tttaccatct ttacagaatt
     1201 ctactgaact tgtaaagaat ccttcgaatg gacctgttct acactcatgg ttcttatctt
     1261 cggttgggta tttgctatag gtatcgccat atgatgatga tgatgagtca gaatagctgt
     1321 tgtatgaatt ttcgtcttgg tatccgtaat tttgagcaga ggctgaattg ctagtgaaag
     1381 cagctaaccc tgaacccaaa attaagctca ttaatagagc cataggaaga tataatttgt
     1441 acatcggaat ctatattcgt ataatattaa tatgcagtaa gtaggatcta ttactaacaa
     1501 aattatcaga attttaaaat aatcaaagtc ttgcaactat aaacgtgtat atgattatgt
     1561 tctattaata atagatttag atatttattt ttattataaa acaatgaaat ataaaatttc
     1621 attttcattt gcgcaagtga tgaatctagt taagttattg tgtctcttga agttaagagt
     1681 gatattatca atacattatt actaagaaaa tattacaaat ttatagctag cgacagaatt
     1741 cgtattgtac agctttgact attatcattg gtattgttgt aattcatatt attattatta
     1801 ttattattat ttcaacaata aaaaattgat aagttctgtt ctataccatt ttttattttt
     1861 ggcttatttg tatctttaaa gcagaatata tttcatattc aactctgtaa ttcatatctc
     1921 ttttctattg tatttaagat tcgttcgttc ttcttatttc gtatcttaat aataaaacac
     1981 tactaggcta tcgattcagc taatttttta gtttcattag aactttaagg tgtattttat
     2041 actat
//
LOCUS       DASVEV010000103         7672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1395636, whole genome shotgun sequence.
ACCESSION   DASVEV010000103 DASVEV010000000
VERSION     DASVEV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395636"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VF222_04805"
     CDS             <1..744
                     /locus_tag="VF222_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141779.1"
                     /translation="DKPVGPPDDKPVGPPDDKPVGPPDDKPVGPPDDKPVGPPAKYSN
                     DIKHKINNEISAITKIIQKYRIGHINKKIIYSAIGIIAVAVIIMIFSQESDFKISPPK
                     FNQAAVTEAKALEISLKNVSVTNIDNNIIKMNTIFDVYNPNEGTLILENINYNVNLDK
                     IRIASGDIGQRPEGFVDSQEGIYPIIGNGTVTLKDEKTIEKDNRIDNVWNKIANKSSS
                     FQVNGSFAYKQTSSFQAHGGEIDFNMVYP"
     gene            819..938
                     /locus_tag="VF222_04810"
     tRNA            join(819..857,880..894,919..938)
                     /locus_tag="VF222_04810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:854..856,aa:Gly,seq:tcc)
     gene            complement(1328..1615)
                     /locus_tag="VF222_04815"
     CDS             complement(1328..1615)
                     /locus_tag="VF222_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141780.1"
                     /translation="MKQLVIAIPFVMTFIYALTLPIEQYIVFGEENSTNSISERIILP
                     YTIQTTTTTTTAVQQDGESDENEPITDLPVIDTPCKSPCPPTAEMCIFMCK"
     gene            complement(1941..2582)
                     /locus_tag="VF222_04820"
     CDS             complement(1941..2582)
                     /locus_tag="VF222_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7141781.1"
                     /translation="MAAGKVIGGIVLIILGIVFFGPSHWANTATYYDLEICNSLTGEP
                     GQGLTQGNYQYCQNMIIVNSISLVGMIVGGIFVIIGLVYVIIGAIRGKQDKKKVKEIK
                     DSNINANSSKELKEIRGEVKKVAQSSMSAPNTDNPIIDNKNNNNDYISRLEKLAEIKK
                     NGIITQEEFNILKVDIIKKFGNASIETTTKSDSKVTNSDSNVTTKNSEEDRVL"
     gene            2832..3575
                     /locus_tag="VF222_04825"
     CDS             2832..3575
                     /locus_tag="VF222_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141782.1"
                     /translation="MTVLILLHSNNGVSYSEEEELSNQTINKNNTNFDISTLVFELGS
                     SPFNLTYADWTAKWWQWAYSIPQNIHPAYDDTGKFCKEGQKEPVWFFPGTYQHSVVRY
                     CETPYGVGILFPILNSECSIAEYPTMKTKDELSNCAKKIQDTIVPEFTTLNSIKIPNL
                     ENYRIQSDIFSFTLPENNILNLPPESTKAVSDGNWVFLKPLPPGYYELKFKGNIKTTS
                     NETNGSTEEDEFAEPIGWNYTTTYMLTVK"
     gene            complement(3628..4236)
                     /locus_tag="VF222_04830"
     CDS             complement(3628..4236)
                     /locus_tag="VF222_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141783.1"
                     /translation="MILLDNETECLPKFISAQKLLEDKFIIEFTSNNDKNNRKIRKTR
                     TIELTIGGDIACFVKPHSDFVCDTIVRGIVPKRHNGLDSPTVFVLITDNKFDFYNITE
                     IADKKYRILNRALERIIVKRVFTIHQLAHFLIIDLEKDIKKYKSKLVVITGDFFLSDS
                     QISKGDKDWLYPQMIKAIKKVRDSIVLVFSPTNLSNLMNYGG"
     gene            4794..5177
                     /locus_tag="VF222_04835"
     CDS             4794..5177
                     /locus_tag="VF222_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX7141784.1"
                     /translation="MFFLFLLIGAAIWKFTHRTKERRYFSAEVKRQVLRDQNYKCAIC
                     KRSTGVWDYDHTDGNRSNNKISNCQALCPNCHAKKTRGLLKAKEKSQSRVRIILIIIV
                     IILFFVVLYYYNMQKNDILLKSEIY"
     gene            6104..6391
                     /locus_tag="VF222_04840"
     CDS             6104..6391
                     /locus_tag="VF222_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141785.1"
                     /translation="MKNQSVLTISFVLIFFIYALTLPIEQYIVFGEENSTNSISERII
                     LPYTIQKTTTIAVQQDGESDENEPITDLPVIDSPCKSACPPNYKMCIYTCL"
     gene            complement(6527..6862)
                     /locus_tag="VF222_04845"
     CDS             complement(6527..6862)
                     /locus_tag="VF222_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141786.1"
                     /translation="MKKKIDYEEVRKYWTEFLRLQNMKDLYNIYRDNEYHLDYLLKIY
                     YFMLRNNIPKKDCEIVLRNVDNVINLNNSVSNLKLECETWQRVKNNHDNYPLEPLPKI
                     NWNYSNYHF"
     gene            7068..7415
                     /locus_tag="VF222_04850"
     CDS             7068..7415
                     /locus_tag="VF222_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141787.1"
                     /translation="MGKNGHKIIVVTTITILVTLAVLPSSVFAENGVIQIQMRGGGIG
                     DDLKISAYTDQNSWEWRESFTIGDTLAKQVNGVSSGDIINVCVINVKDGREDCNSGTF
                     NSNGKVEINIDFG"
BASE COUNT         2771 a         1062 c         1102 g         2737 t
ORIGIN      
        1 gacaagccag ttggaccacc agatgacaag ccagttggac caccagatga caagccagtt
       61 ggaccaccag atgacaagcc agttggacca ccagatgaca agccagttgg accaccagca
      121 aaatatagta atgatattaa gcataaaatt aacaacgaaa tatcagcaat tacaaaaata
      181 attcaaaagt atagaatagg tcatataaat aaaaaaatta tatattctgc tattgggatt
      241 attgcagtag cagttataat aatgatattt tcgcaagaat ctgattttaa aattagtcct
      301 ccaaaattta accaagcagc tgtgacagaa gcaaaagctt tagaaatttc actgaaaaat
      361 gtttcagtta caaatattga taataatatt attaaaatga atacaatatt tgatgtttat
      421 aatccaaatg aagggacttt aattcttgaa aatattaatt ataatgtcaa tttggacaag
      481 atacgcattg catccggaga tattggacaa cgaccagaag gatttgtcga ttcccaggag
      541 ggtatttatc ctataattgg caatggaact gtaacattaa aagatgaaaa aacaatagaa
      601 aaagataaca gaatagataa tgtatggaat aaaattgcta acaaatcttc tagttttcaa
      661 gtaaacggga gttttgcata taaacaaaca tctagttttc aagctcacgg aggagaaatt
      721 gattttaata tggtatatcc ataaattttg atacgaaata attcataatt atttgatggg
      781 aagatttaat atctattgat tttcttaaat aatgaattgc ggtcatcgtc tagtttggtt
      841 aggacactgg ccttccatgc atatatacca tgcgaagtta gcccgcaacc cgggacttta
      901 ttcaaagtaa agcgggaatt caaatcccgg tgaccgcata tacaatggga attcatagac
      961 tagcttttgt tattctatag atgtaatcaa gaaagaagag aagaaggaga agaaggaaaa
     1021 tagttaaaaa atgtaatcat gttcagttaa actatccact atagtaaata acaagattag
     1081 ccttgttcct aacagagaaa attcctctat aatatatgat ttctataact atatgcagga
     1141 aaagaagtga agatgaacaa taattaacac tgaagtaatc aagaattttc agcttctttc
     1201 aatcttttat cagttacatt tttaacgtaa taataatttt ttctgtatat ttaataacaa
     1261 aataatattt ccttgattat cattaggaat gtgttaataa atagcaaata aaaaaaatta
     1321 attaatttta tttacacata aagatacaca tttctgcagt aggtggacaa ggagatttac
     1381 atggggtatc aatgactggt aaatctgtaa ttggttcatt ttcgtctgat tctccatctt
     1441 gttgtactgc tgttgttgtt gttgttgttg tttggatagt ataaggtaga ataattcttt
     1501 ctgatatgga gttggtactg ttttcttctc caaaaacaat gtattgttca ataggtaaag
     1561 tgagtgcata aatgaatgtc attacaaaag gaattgcaat aactaactgt ttcatagtac
     1621 tatttcacta taatatatat atcattcaat taataaaaag attttaatat aaaattagtc
     1681 aataatatct ataacatttt tacatattca taagtttaag tttatagaat aaaacttgta
     1741 taagatttag taacaaaaaa tactatgaca tattttcgga attgatttca taataaaaat
     1801 tagcaaatga ataacacaat aaccatcctc taataaaatt tagtattgtt gtcattacag
     1861 atatacttta gcattttaca atatatataa gctttaggtt ttattgaata ataataaaaa
     1921 tatttaattt tttgtttttg ttataatact ctatcttctt cagaattttt agtagtcaca
     1981 ttagaatcac tatttgtgac cttagaatct gatttagtag tagtttcaat agaagcatta
     2041 ccaaatttct ttattatatc aacttttaaa atattaaatt cttcttgtgt tataattcca
     2101 ttttttttta tttcagctaa tttttctagc cttgatatat aatcattatt gttgtttttg
     2161 ttatctatta ttggattatc tgtattaggt gcagacatag atgattgagc tactttcttt
     2221 acttctcctc ttatttcttt caattcttta gatgaattcg cgttaatatt ggaatccttt
     2281 atttctttaa ctttcttctt atcttgtttt ccacgaatag cgcctattat tacgtatact
     2341 aatccaatta tgacaaatat tccgccaact atcataccga ctaatgaaat tgaatttact
     2401 attatcatat tttgacaata ttgatagttt ccttgagtaa gcccttgtcc aggttctccg
     2461 gttaatgaat tgcatatctc aaggtcataa tatgtcgctg tatttgccca atgagaaggg
     2521 ccaaaaaaga cgattcccaa aataattaaa acaataccgc ctattacctt tcctgctgcc
     2581 atgatacttg tctttgaaag tcattattta tctatttcat atgataggag tttttgaaaa
     2641 agcagatgaa acgtaatact gttggaaaat aaaactactc aactattttc aattgttgtt
     2701 gcaatttcaa tatccaaatt tattaaaaat aatttaatat tgacatagca tcgagaaaga
     2761 aaaagttctt aatatatctt aaaataaaaa ataagaatat gataaaattt atcctaattc
     2821 taagttcatt tatgacagtt ttaattcttc tacattcgaa taatggagtt tcgtatagtg
     2881 aagaagaaga actctctaat caaactatta ataaaaataa taccaatttt gatatttcta
     2941 cattagtttt tgagcttggt tctagtcctt ttaatcttac atatgcagat tggactgcaa
     3001 aatggtggca atgggcatat tcaattccac aaaatattca tcctgcatat gatgatactg
     3061 gaaaattctg taaagagggt cagaaagaac ctgtctggtt tttcccaggt acatatcaac
     3121 attcagttgt acgctattgt gaaacccctt atggtgtagg aatactcttt cctatattaa
     3181 attctgaatg ttcaattgct gaatatccaa caatgaaaac aaaagatgaa ttaagtaatt
     3241 gtgctaaaaa aattcaagac acaatagttc cagagtttac aacacttaac agtataaaaa
     3301 taccaaatct agaaaattat cgtattcaat cagatatttt tagttttacg ttaccggaaa
     3361 ataatatttt gaatttacca ccagagagta ctaaagcagt ttctgacgga aattgggtat
     3421 ttttaaaacc tttacctcct ggatattatg aacttaaatt taagggaaat attaaaacta
     3481 caagtaatga aacaaatggt agtacagaag aagacgaatt tgcagaacct ataggttgga
     3541 attatactac tacatacatg ttaacggtaa aataataatt taaatcctgg gctaaataga
     3601 atctcttgta agaaaattaa tttgaattta tccaccataa ttcattaaat tggacaaatt
     3661 tgtcggagaa aagacaagaa caatagagtc tctaaccttt ttgattgctt taatcatttg
     3721 aggatatagc cagtctttgt ctccttttga aatttgtgaa tctgaaagga aaaagtcacc
     3781 tgttataacc actaattttg atttgtattt ttttatatct ttttctaaat ctattatcaa
     3841 gaagtgtgcc agttgatgta ttgtgaaaac cctttttact ataatacgct ctaatgccct
     3901 atttaatatc ctatattttt tatcagctat ttctgttata ttgtaaaagt caaatttgtt
     3961 gtcagtaatc aaaacaaata ctgttggaga atctaaacca ttatgacgtt ttggcactat
     4021 acctctgaca atagtatcac aaacaaaatc tgaatgaggt ttaacaaaac aagctatgtc
     4081 acctcctatt gttagttcaa ttgttcttgt ttttcttatc ttcctgttgt ttttatcatt
     4141 attagatgta aattcgatta tgaatttatc ttctaagagt ttttgagctg atataaattt
     4201 aggtaagcat tctgtttcgt tatctaataa tatcaactac aacaaaccaa aacatcattc
     4261 tatttatttt agcaatctag gtgtagagag ggagagagag tagatttgta aagttaattt
     4321 tcattttatt tttcttctga ttttattgat tacttcactt ctacggtgaa aatatccttt
     4381 agtcttctct gtttatatct taaacctaga ttataccata ttatgattag gatattgttc
     4441 catcactcta tcatagcatt ctatatccct atttccttgt aattcgtctg gaaatctctc
     4501 ccagaaccat tctaaaaaag tttgcatgat taatgacttt gatacgtaaa agaaaatgat
     4561 tttaaagaat ttactcattt ttatctataa ctttcatagt atctttatat aataacaata
     4621 tcatttacag attgattcgc tctcgcatgt tatgggtttt attattaaca atttttttat
     4681 ttatttttat aatagtttcc gttgtatata ataatattat tttcagtcaa actgatactt
     4741 ttgaagaacc aatttcaaag gatttcgcac atgaggagga gtttattgtt atcatgtttt
     4801 ttctttttct tctaattgga gctgctattt ggaaattcac tcatagaact aaagagagac
     4861 gctatttttc tgcagaagta aaaagacaag tattaagaga ccagaattac aaatgtgcta
     4921 tatgtaaaag aagtacagga gtttgggatt atgaccatac ggacggaaat aggtcaaaca
     4981 acaagatatc caattgtcaa gcattatgtc caaattgtca tgctaaaaag acacgtggtt
     5041 tgctaaaggc taaagaaaag tctcaatccc gagtaagaat cattctcatt ataattgtaa
     5101 ttatattatt ttttgtagtt ttatattatt ataatatgca gaagaatgat attttattga
     5161 agagtgaaat atattagata ttacataatt aaaatagaga aaattaattt agcatgattt
     5221 tagctgtatc gaatttaata attgccttag gctattgtca gaagaatatt tcaataacgg
     5281 tcatttgaaa atgttttacc atattttttg ataaattttg tttctgttag ttcatcagat
     5341 gtttaaatca gtcagttctt gattttccgt tggtagtaaa atgggtgcaa gatttctcaa
     5401 acacccattg aatttggtta taacgatttg atggagctag taacacagta gggtatgata
     5461 aaaaatgttt ggtttcagtt ggtaaactat aaagctaata agtttgtaat agtataaatg
     5521 cttcaaggtt taaggcaatg aatacttaaa atcacagacc atttttaaat gaatcaaatt
     5581 taaggaaaaa tattatcaag tggcttgaca aagaatgaga gttacaaaat ttcttaacta
     5641 atagtcctta tgccaaactt atagaccaca ttactacatc tgttaatagt aaaaaagatt
     5701 tgaaagacta aacggaactt taagagaacg tgtaaaggtt caaagaggtt gtaaatccat
     5761 aaattgctat tgcagaagga caaagaatat actacaactt tgtaaaaaca catcaagcat
     5821 tagagggaaa aataccagag acaaaatacg gtatcaaaat tgacagtaat aacaaatgga
     5881 gaataatagt tcgtgtattg attatctgta ttctaccaac tgagttccaa caaggtttta
     5941 aaccatctga tgaaccaaca gaaccaaata atatatttaa caattaattg agattgtaaa
     6001 ttctaacata tttcatttag cagatttaaa tgttaatctc actgaagaat ttatttaaaa
     6061 tatttttatt aggagaatgt aatagctatg ttattgaaat agtatgaaaa atcagtcagt
     6121 ccttacaatt tcttttgtgc taatattctt tatttatgca ctcactttac ctattgaaca
     6181 atacattgtt tttggagaag aaaacagtac caactccata tcagaaagaa ttattctacc
     6241 ttatactatc caaaaaacaa caacaatcgc agtacaacaa gatggagaat cagacgaaaa
     6301 tgaaccaatt acagatttac cagtcatcga ttctccatgt aaatccgctt gcccacctaa
     6361 ttataaaatg tgtatctata cgtgcctata aaaataatat atatatatat atatatatat
     6421 tcatattatt tttattgaat taaaataatt ctcattcaat ttggttaata taaataatta
     6481 acaactcact aaaatgagta ttatattaat aaagtattgt agaaaattaa aaatgataat
     6541 ttgaataatt ccaattaatt tttggtaatg gctctagtgg gtagttatca tgattatttt
     6601 taactcgctg ccatgtttca cattctaatt taagatttga aacagaatta tttaaattta
     6661 taacattatc tacatttctc aaaacaattt cacaatcctt tttgggtatg ttatttcgta
     6721 acataaagta gtatattttt aacaaataat ctaaatgata ttcattatct ctgtatatgt
     6781 tatacaagtc tttcatattt tgtaatcgca aaaactcggt ccaatacttt ctgacctctt
     6841 cataatctat tttctttttc aaatttgttc aaaagaattt tagacattcc taataagctc
     6901 ctaaaaaaga agttaataaa ttgtatcact attgttatca tttgttattt tatgttatca
     6961 actaattgtc actgttatca tctattttga attaatcaga caatatccta aatttgaaac
     7021 cgattgttat ataaaatatt atatacataa tttgataaga tttgctaatg ggaaaaaatg
     7081 gtcataaaat tattgtagta acaacaataa caatactagt cacgttagct gttcttccat
     7141 ctagtgtctt tgcagagaat ggagtcatcc aaatacaaat gcgtggggga ggaataggag
     7201 atgatttgaa aatatcagct tatactgacc aaaattcttg ggagtggaga gaatctttca
     7261 ctataggtga caccttagct aaacaagtga atggagtttc tagtggagat ataattaatg
     7321 tatgtgtaat aaacgttaaa gatggaagag aagattgcaa ttcgggtact tttaacagca
     7381 acggtaaggt agaaataaat atagattttg gttagttagt tagttactat ttaaatatta
     7441 ttttattttt tataacatta gattcttatt tttgcacggc tatagacatt actctctaga
     7501 aataaatgta atttcacaac gcagtatcat gatgaaaagc tcctcgttta tcaatcgtta
     7561 catctactgt cccgtcatac tttggcttga ctgctaattg tgctggtgta tatatcaaat
     7621 gttctttagt aaatgatgat aattcaaaat catcagtcat atacaatgca ta
//
LOCUS       DASVEV010000104        10190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_35801, whole genome shotgun sequence.
ACCESSION   DASVEV010000104 DASVEV010000000
VERSION     DASVEV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35801"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF222_04855"
     CDS             complement(<1..888)
                     /locus_tag="VF222_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX7141788.1"
                     /translation="MASLDNNNNKFKQEIEVKGHLIDSMILTSIFDNVMDLKGEFRVL
                     EFTVGKNKTDPSYARLIVEGKDEKHLNQILESIYRAGAQPISTQNVLLEPAPNDMVMP
                     DLFYSTTNKPTQIFYDGIWIDVKNIMMDKCIVIDTHNKTAECKMIRDIKKDDMIVIKE
                     RGIRIIPDERPRQGVDIFQFMSSTTSSERPTQHIIKKVAYDIYEVAARQKGKIVVVGG
                     PVIVHSGASQSLSKLIEMGYIHGLLTGNALAVHDIENALLGTSLGMNIDDGTLAVRGH
                     RNHMQSINEVFKAGSIKNMV"
     gene            complement(933..1259)
                     /locus_tag="VF222_04860"
     CDS             complement(933..1259)
                     /locus_tag="VF222_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141789.1"
                     /translation="MQYFTALKLGEKRVKQSQDLLNLYTGDAAMPALALKDTKTNVWF
                     PVGEENLIAVFKEASGYVIVICDKNGIAKTIAQWFSEEDKNNIVSRIKKDHSLSEYTG
                     KLSLPI"
     gene            complement(1272..1826)
                     /locus_tag="VF222_04865"
     CDS             complement(1272..1826)
                     /locus_tag="VF222_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7141790.1"
                     /translation="MNDYKFSNLIDYILKLKTIKRTGWISKAKILKAESVADHSYSLT
                     ALSMVFSDLLGLDTEKVMKLCIIHDLAESIIGDYMPEEISILEKHIKEDNAMKVIISS
                     FPNKIAILYSELWKEYTLNQTKEVHLVKQLDKIEMFLQANQYFRNGYSFEFLSPFLKI
                     LDNIEESDSQLTLANILKHLDKNL"
     gene            complement(2121..2693)
                     /locus_tag="VF222_04870"
     CDS             complement(2121..2693)
                     /locus_tag="VF222_04870"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7141791.1"
                     /translation="MKIYTKSGDKGLTGLIDGKRIPKSDIRIIAYGSVDELNSYIGLS
                     ISHLSQHKTNNNNNQSFSDIIMTLNRIQNELFIIGSDLADPDLSKSTSLRVQSTMITI
                     LENDIDNYEKELSPITYFILPGGCVESSTLHIARSITRRAETNVSKLLLEDVINNLVL
                     IYLNRLSDLLFVLSRTVNKRFQISDIAWKS"
     gene            complement(2775..2945)
                     /locus_tag="VF222_04875"
     CDS             complement(2775..2945)
                     /locus_tag="VF222_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141792.1"
                     /translation="MNNSPNPEPNQVIETAEERKQRFLLEFDQPFKCDKCNERFKKKK
                     YLREHKAEVHSY"
     gene            3121..3227
                     /locus_tag="VF222_04880"
     tRNA            join(3121..3151,3185..3227)
                     /locus_tag="VF222_04880"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3188..3190,aa:Phe,seq:gaa)
     gene            3269..3433
                     /locus_tag="VF222_04885"
     CDS             3269..3433
                     /locus_tag="VF222_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141793.1"
                     /translation="MESIIQIFRRIFCSHDDSFSFRVYNDFDFSEYNKCTKCGKTLGY
                     FKGFDEDLRG"
     gene            3441..3647
                     /locus_tag="VF222_04890"
     CDS             3441..3647
                     /locus_tag="VF222_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141794.1"
                     /translation="MVLRINLQILIISWWLGAATALLSFLLPIYTHHIIVGGIGWSII
                     FLSTILIIYEIKKIKEEDKRTKSL"
     gene            complement(3654..3908)
                     /locus_tag="VF222_04895"
     CDS             complement(3654..3908)
                     /locus_tag="VF222_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7141795.1"
                     /translation="MPTAYILVNCTLGSEEKIISELTNLPDVKEVRGTYGVHDVFVKV
                     RSSDTESMNNTITNKIRKVPGITSTVTLVVIEEQGGKESL"
     gene            4062..4970
                     /locus_tag="VF222_04900"
     CDS             4062..4970
                     /locus_tag="VF222_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141796.1"
                     /translation="MRLPLKDSDYFVTRVNETVKTIDEEFFNFLKPKLQMKKVGVMLG
                     DGTITNAETFDPAEITNEFRRILQSLKDWTFTDISDSKTEDLYRLYFQITCNVDKFYI
                     YGYFGIQFHALHYYKLDKEVIKIQKELIKLNQDVSDSFISLADKGNTIIEEELRRQGL
                     DNLSFEELFTKIYDNSNLMDNLEQKVLKLEKRFPVVQDSENKREQLISELNDMIIKLY
                     HIIPIKIDYNRLMQGEEGVVSYFEIEIIKNKKTGKREPYLDTKKFNKEITEIVTSRLS
                     EIVSSLKELRTNANKKVDEDLKNIPG"
     gene            5058..6299
                     /gene="ilvA"
                     /locus_tag="VF222_04905"
     CDS             5058..6299
                     /gene="ilvA"
                     /locus_tag="VF222_04905"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX7141797.1"
                     /translation="MNNELNLETKNLPTYDEIIKGRELLQNFVGKTPLYKSNTFSNLI
                     GTNIFLKNEAFQKTGSFKVRGALYKVNSLSEEEMKYGIIAASAGNHAQGVAYAATIRK
                     IPCTIVMPMSASPAKIAATRSYNARVILKGADYDEAWIAAKEIARNENKTIIHAFDDP
                     EVIAGQGSIGLELLEDLPDLDEIYLPVGGGGLAAGVAIAIKSKNRNIKIIGVESKSFP
                     AMKESLEKGSLQTKKGEFTIADGISVKSPGKLTFDIAKKYLDDIVLVDDDSIVKTMFL
                     LMERSKLVVEPAGAASLAYLLSNGHKNRNKKIVSILSGGNVDMYLLGQVVAKGLLQMN
                     RLLKIFIQLTDKPGALKTVVDGISDLSINIVEVVHDRLSANISAGTAGVYLSLELENA
                     KHCELLISFMKKKGIAFQIIT"
     gene            complement(6358..7407)
                     /locus_tag="VF222_04910"
     CDS             complement(6358..7407)
                     /locus_tag="VF222_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX7141798.1"
                     /translation="MGKANKPQRIFGTVRAFSERGTMLSKHDLESFSDSKTLEEFVTR
                     LKNTSYVDSLSKIQQPITSEKIENALREDLFNFHSSMAKISKGDLVNAYYKKYIIWNL
                     KIILKSKALGKTYDDIFPHINMQAEELIGKRDIIIKALVAKDLDETVSALQTSEFGNE
                     ISSAAQLYKENSNIQIFELYLDKIYYKGLVSAFANIGKPADLQPLVSLDLDYYNVLST
                     IRAKYWKLPEEHIKSLRIATTSRVNEDVLNKMIHADSVKQAISEFSKTVYKDIVPNQG
                     NENDTTIISQIESSFEELIHRKSMALYGRIFSYAPMTAAIKLKMTEIKNLVAIAFGIE
                     QKMNPKNIIDRLLLI"
     gene            complement(7502..7870)
                     /locus_tag="VF222_04915"
     CDS             complement(7502..7870)
                     /locus_tag="VF222_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141799.1"
                     /translation="MESNVSTVERIINTLSEFETNVENINSSIEELKKRMLSHSNSEI
                     EQLREKIIKLANEEAKGILDNAKKESEKESLLIQEQAEKNYNLIKNNIEKNHDKAIDK
                     VISMILGNTDNDESNFGGRT"
     gene            complement(7940..9202)
                     /gene="truD"
                     /locus_tag="VF222_04920"
     CDS             complement(7940..9202)
                     /gene="truD"
                     /locus_tag="VF222_04920"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX7141800.1"
                     /translation="MIKSVPEIDFHAGMECYCTNFPGINGTIKETNEEFQVSEIIDYT
                     FLKPPYFSSVQNNEHKFPLYLLEKHNIDSNHAIIEIKKQSGLKLRIMGIKDAKAETSQ
                     YASCEQTRNLPKYIITPKTKLTLLGFLRTPLSKSALIGNRFKIKINQTSDNNIQPFLL
                     ELNKIANFYGLQRFGSERMVTHLVGKALLERNYNKAIEYLLSYTSKYDSKFSKEIREK
                     SRDPKNYQKLIKILPKGMDLERLILDTLVKGKDSIAVIRSVPINIRRLFVQAFQSYVF
                     NKCLSNALLSNEDIQKCKDGDLCFEIEKPLVFGKIRRFREGIDSNNDVIPAIRLVGYN
                     YQPGKNRFDHITKRILSDENISPSYFYLKDLQELSIQMGFRQTLLCCSEFSHFDRLDL
                     SFKLPKGSYATTLLRELIKPVDPIKAGF"
     gene            9299..9688
                     /gene="hsp14"
                     /locus_tag="VF222_04925"
     CDS             9299..9688
                     /gene="hsp14"
                     /locus_tag="VF222_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HEX7141801.1"
                     /translation="MGIATYMAKEVMKEINNKSKEFYEFILPPVDMIEDSNDLLVIID
                     LPGFNKNDITLRINKNILSIKANKPDNEYRGTVYLKHRPTKIDKKVILPFLVDEDEEI
                     TGTANYSDGIITLRIPSKNLGNRILIS"
     gene            complement(9767..>10190)
                     /locus_tag="VF222_04930"
     CDS             complement(9767..>10190)
                     /locus_tag="VF222_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7141802.1"
                     /translation="ETTHSLKLNQSIYADATYVESIQNNPDKVLVDSRERLNFLSEHI
                     PSAKNIPYTMLGNDNHILRSVDELKRFVENRGIPQDSEIITYCGSVGTLSGLAFYAFK
                     LAGIKNIKLYSKSFKDWKSLGKPKIEFKDANYWDLSAE"
BASE COUNT         3569 a         1461 c         1470 g         3690 t
ORIGIN      
        1 taccatattt ttgatggaac cagctttaaa aacttcatta atagattgca tatgattcct
       61 atgaccacgc acagccaaag ttccatcatc tatattcata cctaaagaag ttcctaaaag
      121 tgcattttca atatcatgaa cagctagtgc atttccagtc agtaatccgt gtatatatcc
      181 catttctatt agtttagata atgattggga ggctcctgaa tgaactatta ctggtcctcc
      241 cacaactact attttccctt tttgtcttgc tgctacttca taaatatcat atgcaacttt
      301 tttaataata tgttgagtag gtctttcgct tgaagtagtg ctgctcatga attgaaatat
      361 atcaactccc tgacgaggac gttcatcagg tataatacgt atacctctct ctttgattac
      421 aatcatatca tcttttttta tatctctaat cattttacac tctgctgttt tattatgtgt
      481 atctataact atgcacttat ccatcataat atttttgaca tctatccaaa taccatcata
      541 aaaaatctgt gttggtttat tggttgtact ataaaataag tcaggcatga ccatatcatt
      601 tggggcaggt tctaacaata cattttgtgt agaaatcggt tgagctcccg ctctgtaaat
      661 agattctaat atttgattaa gatgtttttc atctttacct tcgacaataa gtctagcgta
      721 actaggatca gttttattct taccaacagt gaattccagt actctaaatt ctccttttaa
      781 atccattaca ttatcaaaaa tactagttaa aatcattgag tctataagat gtcctttgac
      841 ttcaatttcc tgcttaaact tattattatt attatctaat gacgccaaat tgtaatatag
      901 atagatatat tttttaggta attaagcatt aatcaaattg gaagagaaag tttcccggta
      961 tattcggata gactatggtc tttcttgatc cgtgatacaa tattattttt atcttcttct
     1021 gaaaaccatt gtgcgatagt tttagctatt ccatttttat cgcaaattac aataacataa
     1081 ccactggctt ctttgaatac agcaattaag ttttcttctc caacaggaaa ccatacattt
     1141 gtttttgtat ccttcagagc taaagcaggc attgctgcat ctccagtata taaatttaac
     1201 aaatcctgcg attgtttaac acgtttctct cctaacttga gagctgtaaa atattgcata
     1261 acaagaatat attataaatt cttatctaaa tgttttagta tatttgcaag agtaagttgg
     1321 gaatcagatt cttcaatatt atcaaggatc tttaaaaacg gagataaaaa ttcaaatgaa
     1381 taaccatttc taaaatactg attagcttgt aagaacattt ctatcttatc tagttgcttt
     1441 acaagatgta cttcttttgt ttgatttaat gtatattctt tccataactc tgagtaaagt
     1501 attgctattt tatttggaaa agaagatatt attactttca ttgcattatc ttctttgata
     1561 tgtttttcta aaatagaaat ttcttcgggc atataatctc ctattatgga ttctgccaag
     1621 tcatgtatta tacacagttt cattactttt tccgtatcaa gacctaataa atcagaaaaa
     1681 accattgaca aagcagttaa tgaatagctg tggtctgcaa ccgattcagc ttttagaatt
     1741 ttggctttgg atatccatcc tgttctttta atagttttta acttaagaat atagtctata
     1801 agattactga atttataatc attcatttta ccttcataat tatatatcaa ttaaattaaa
     1861 attattcata tcttattatt attatagtat aataataaaa aatagaggta agacagacaa
     1921 gattatagga aagagttcta aatttagtaa atgaaaatat gtcattgcta tgataataaa
     1981 tggatttaga tacaaatcaa atagatatgc taagcctgac gtaacaatag ctaacattac
     2041 agttataatg attattatta taacattttg tatttgtccc atttaaagac actactttcc
     2101 atttctacta aataaatgat ttaagatttc caggctatat ccgatatttg gaatctttta
     2161 tttactgttc tacttagtac aaaaagcaga tccgaaagtc tattcaaata tattagaacc
     2221 aaattgttaa ttacatcttc caacaataat tttgaaacgt tggtttctgc tcttcgtgtg
     2281 atgcttctag caatatggag agtagaggat tcaacacacc ctcctggaag aataaaatag
     2341 gtaataggag ataactcttt ttcatagtta tcgatatcat tttccaatat tgtaatcatg
     2401 gtagattgaa ctcttaaaga agtagacttt gaaaggtctg gatcagctaa atcagaacct
     2461 ataatgaata actcattttg aattctatta agcgtcatta taatatcaga aaaagattga
     2521 ttattgttat tattagtttt atgctgtgac aaatgagata ttgataaacc gatatatgaa
     2581 ttcagttcat ctactgagcc ataggcaatt attcttatat cactctttgg aattcttttt
     2641 ccatcaataa ggcctgttaa tcccttgtct ccggatttcg tatatatttt catatataat
     2701 aaatctgaaa ccagagaaat aatatcctat actttaactg acgaattgat taattaactg
     2761 acgaattgat taatttaata agaatgtact tcagctttat gctctcttag atatttcttc
     2821 ttcttgaatc tttcattgca tttgtcacat ttaaaaggtt gatcaaattc caagagaaat
     2881 cgttgtttcc tctcttctgc agtttcgatt acctgattgg gttcaggatt aggagaatta
     2941 ttcattatga tttctcctag aaaataattt ttaatcttta tttagtttta gaaaaatagt
     3001 tatgttagat tgattatgta aaaaattata tatttttttt atgaactttt tttattaatt
     3061 tacaatagca atacctacaa aatagaatat atattgagtc aatttaaatg attattcaat
     3121 gccgtcgtag ctcagcctgg gagagcactc gacttgattg attatctaat tattcaaggg
     3181 tgaagctgaa gaccgagctg tcgtgagctc aaatctcacc gacggcatta atttatatta
     3241 tatcagagaa tttagaaatg tcgtattatt ggaaagcata atacaaatat ttagaagaat
     3301 attttgttcg catgatgatt ccttttcctt tagagtgtat aatgattttg attttagcga
     3361 atataacaaa tgcacaaaat gtggtaaaac acttggctat ttcaagggat ttgatgagga
     3421 tctaagagga tgatttttga ttggtactaa ggattaatct tcaaatccta ataattagtt
     3481 ggtggttggg tgcagctaca gcattgctct catttttact tccgatatat actcatcata
     3541 ttatagttgg aggaattgga tggtcgataa tttttttaag tactatacta ataatttatg
     3601 aaataaaaaa gataaaagaa gaagataaaa ggactaaatc actataaaac agattaaaga
     3661 ctttcttttc cgccttgttc ctctattact acaagagtaa cagtagatgt aatgccaggc
     3721 acttttctta ttttgtttgt tatagtatta ttcatagatt ctgtatcact agatctaacc
     3781 tttacgaaaa catcatgaac tccataagtt ccccttacct cttttacatc tggcaaattt
     3841 gttaattcac tgataatttt ttcctcagaa cctaatgtac aattaactaa aatatatgct
     3901 gttggcatag cttaatttag ttttattaat attttagttt tgtttttttt agcaatgtat
     3961 aaccgatagg atgataaatt tttagttaaa ttagcaataa aattaataat tattacacaa
     4021 tattacaaat ctatatgtat tgaaaattat aatttaaaaa catgaggtta cctctgaaag
     4081 atagtgatta tttcgttaca agagttaatg aaactgttaa aaccattgat gaagaattct
     4141 ttaatttttt aaaaccgaaa ttgcaaatga aaaaagttgg tgtaatgtta ggggatggga
     4201 caataacaaa tgcagaaact tttgatcccg ctgaaattac aaatgaattc agacgaattt
     4261 tacaaagtct aaaagattgg acttttacgg atatttccga ttctaaaacc gaagatttgt
     4321 atagattgta ttttcaaata acatgtaatg ttgataaatt ctacatatat ggatattttg
     4381 gaatacaatt tcatgcttta cattattaca aattagataa agaagttata aaaattcaga
     4441 aagaattgat aaaattaaat caagatgtat cagactcttt tatttcactt gcggacaaag
     4501 gaaataccat aatagaagag gaattacgaa ggcagggatt ggataaccta tcatttgaag
     4561 aattatttac taaaatatac gacaatagta atttaatgga caatcttgaa cagaaagttt
     4621 taaaactaga aaaacgattt cctgtagttc aagacagtga aaataaaaga gaacaattga
     4681 tttcagaatt aaatgatatg attattaaat tatatcacat aatacctata aaaatcgact
     4741 acaatagact aatgcaagga gaggaaggag ttgtatcata ttttgaaatt gaaattatta
     4801 agaataaaaa aaccggaaaa cgcgagccat atcttgatac taaaaaattt aacaaggaaa
     4861 tcactgaaat tgtaactagt agattaagtg aaatagtatc ctcattgaag gaattaagaa
     4921 ctaatgctaa taaaaaagtt gatgaagacc taaaaaatat accaggatga gatttactaa
     4981 taatagtaga tataacaata gtataaagaa atagtaaatt ataattacaa tagatatatc
     5041 taatctaaga tataataatg aataatgaat taaatttaga aacaaaaaac ttacctactt
     5101 atgatgaaat aattaaaggt agggaattat tacaaaattt tgtagggaag actcctcttt
     5161 ataaatcaaa tactttttct aatttaattg gtacaaatat tttcttaaaa aatgaggctt
     5221 ttcagaaaac tggatcgttt aaggtaagag gagcccttta caaagtcaac tccctctctg
     5281 aagaggaaat gaaatatgga ataatagctg cttctgcagg aaatcatgca caaggtgtcg
     5341 cttatgcagc aactattaga aagattccat gtacaatagt aatgccaatg agtgcatctc
     5401 ccgcaaagat tgcggctacc agatcatata acgcacgagt tatcctcaaa ggtgctgatt
     5461 atgacgaagc atggatagca gctaaagaga tagctcgaaa tgaaaataag acaattattc
     5521 atgcatttga tgacccagag gtaatagcag gacaaggttc aataggctta gaattattag
     5581 aggacttgcc agatttagat gaaatttatc ttcctgtagg tggcggtgga ttagccgcag
     5641 gagttgcaat tgctataaaa tcaaaaaaca gaaatatcaa gataattgga gtagaatcta
     5701 aatcttttcc agcaatgaaa gaatctcttg aaaaagggtc acttcaaaca aaaaaaggag
     5761 aatttacaat tgcagatgga atatcagtga aatctccagg aaaattaacc tttgatatag
     5821 caaaaaaata tttggatgat attgtattgg ttgatgatga ttcaattgtt aaaactatgt
     5881 tcttattaat ggaacgatcc aaactagtag tagaacctgc aggtgctgca agtcttgcat
     5941 atttactgag taatggacat aaaaatagaa ataagaaaat agtatcaatc ttatctggtg
     6001 gtaatgtaga tatgtacctg cttggacaag tggtggcaaa aggtctttta caaatgaaca
     6061 gattgttaaa aatatttatt caattaacag acaagccagg agcattaaag acagtagtag
     6121 atggtatttc agatttaagt ataaatattg tagaggtagt tcacgatagg cttagtgcca
     6181 atatatcggc aggaactgca ggtgtttatc taagtttaga attggaaaat gcgaaacatt
     6241 gtgaactttt aattagcttt atgaagaaaa agggtattgc ttttcaaata attacataat
     6301 agataaaata aataataata atttaaataa ttaattatca aatataaaag agatctatta
     6361 aattaataat agtcgatcta taatattttt gggattcatt ttttgttcaa ttccaaaagc
     6421 aatagctact aggtttttaa tttctgtcat ttttagttta atagctgcag tcataggtgc
     6481 atagctaaat attcttccat acaatgccat tgattttcta tgtatcaatt cttcaaagct
     6541 cgattcaatt tgtgaaatta tagtggtatc attttcattt ccctgattag gaactatatc
     6601 tttataaaca gttttggaaa attctgatat tgcctgtttt acactatctg catgtatcat
     6661 tttatttaga acatcttcat tcactcgaga ggttgtggca atccttaatg atttaatatg
     6721 ttcttctgga agtttccagt attttgccct aatagtgctt aaaacattat aatagtctaa
     6781 atcaagagaa actaaaggct gaagatcagc aggcttacct atattagcaa aagcgctaac
     6841 caagcctttg tagtaaattt tatcaagata gagttcaaaa atttggatat tactattttc
     6901 tttgtatagt tgagcagctg aagaaatttc attaccgaat tctgaggttt gtaatgcaga
     6961 tacagtctca tcgagatctt ttgcaactag agctttaata ataatatccc tttttcctat
     7021 tagttcttct gcttgcatat ttatatgcgg aaaaatatca tcataggtct ttcctaaagc
     7081 tttacttttt aaaattattt tgagattcca gatgatgtat tttttataat atgcatttac
     7141 aagatctcct ttagatattt tggccataga agaatgaaaa ttgaaaagat cttctcttag
     7201 tgcgttttca attttttcgg aagttatagg ttgttgaatt ttactcaaag aatcaacata
     7261 tgaagtattt tttaatcgag ttacaaattc ttctaaagtt ttggaatcag aaaatgattc
     7321 taaatcatgt ttagatagca ttgtaccacg ttcagaaaaa gctctaactg taccaaaaat
     7381 tctttgaggt ttgttagctt ttcccaattt tagtcatcta ccctttataa cactacttta
     7441 ttaattaaat atttttttat gataacaaga aagaaatatg tataaacaat agctataata
     7501 tctaagttct gccgccgaaa ttagattcat cattatctgt atttcctaaa atcatactga
     7561 ttaccttatc aattgccttg tcatgatttt tttcaatatt gttttttatc aggttataat
     7621 ttttttcagc ctgttcttgg attaagaggg attccttttc agattctttt ttagcattgt
     7681 ctaaaattcc ttttgcttct tcatttgcta atttaataat cttttctcgc aattgttcta
     7741 tttcgctatt agaatgagac agcatacgtt tctttaattc ttcaatacta gaattaatat
     7801 tctcaacatt agtttcgaat tctgaaagag tattgattat tctttccacc gtggaaacgt
     7861 tggattccaa ttcatcaaag ttgcaattta aaggtatatt aaacccttcc ttttaattaa
     7921 tcaaattact ttcaagtttt caaaatcctg ctttaattgg atctactggt tttattaatt
     7981 cacgtagtaa agttgtggca taagatcctt ttggtaattt aaatgacaga tcaagcctat
     8041 caaaatggct aaattcacta caacatagca aagtctgtct gaatcccatc tgtatactta
     8101 attcttgcaa gtcttttaaa taaaaataac ttggcgaaat attttcatcc gataaaattc
     8161 ttttagtaat atgatcaaaa cgattttttc ctggttgata attataacct actaacctaa
     8221 ttgctgggat aacatcatta ttggagtcta ttccttctct aaaccttcta attttaccaa
     8281 aaactagagg tttctctatt tcaaaacata aatctccatc tttacacttt tggatatctt
     8341 cattggacaa aagagcatta ctgagacatt tattaaaaac gtaagactgg aaagcttgaa
     8401 caaaaagtct tctaatattt atcggtactg atcgaataac tgcaatggaa tctttacctt
     8461 tcactagtgt atctaaaatg agtctttcta aatccattcc tttaggcaaa attttaatca
     8521 gtttttgata attcttagga tccctacttt tttctcttat ttcctttgag aacttggaat
     8581 catatttgga agtataagag aggagatatt caatagcttt attataattt ctctcaagta
     8641 atgctttacc aactaaatga gttaccattc tttcacttcc aaatctttgt aaaccataga
     8701 aattagcaat tttatttaat tctaataaaa aaggctgtat attattatca gaagtttgat
     8761 ttatttttat tttgaaccta tttccaatga gtgcagattt ggataatgga gtacgaagga
     8821 aacctaataa ggttaatttt gttttaggtg ttataatata tttaggtaaa ttccttgttt
     8881 gttcacaact agcatattga ctagtttctg cttttgcatc ttttattccc attattctca
     8941 gttttaaacc tgattgtttt ttaatttcaa ttatcgcgtg atttgaatca atattatgtt
     9001 tttccaataa ataaagtgga aatttatgtt cgttattttg aaccgaagaa aaatatggtg
     9061 gttttaaaaa tgtataatct attatttcag atacctgaaa ttcttcatta gtttccttta
     9121 ttgttccatt tatacctggg aaatttgtac aataacattc cataccagca tgaaaatcta
     9181 tctcaggtac agattttatc acaaaattaa cctataccaa attaaatata aacaatacaa
     9241 cagttatatc aatcaacaat aaataaagga tttatattca aatagcatat taataattat
     9301 gggtatcgca acatatatgg ctaaagaagt tatgaaagaa attaataata aatcaaagga
     9361 gttttatgaa tttattttac ctcctgtaga tatgatagaa gatagtaatg atttacttgt
     9421 aattatagat ttaccaggat ttaataaaaa tgacataact cttagaataa ataaaaatat
     9481 actctctatc aaggccaata agcctgataa cgaataccga ggtacagttt atttaaagca
     9541 tcgacctaca aaaatagata aaaaagttat attgcctttt ttagttgatg aggatgaaga
     9601 aattactgga actgctaatt attctgatgg aattataacg ttgagaattc ctagcaaaaa
     9661 tttgggaaat cgaatactga tttcctaata aatatattag agaacttaat cctttgatac
     9721 aaaattaaaa ttttaacaaa ttctttataa ttttttgttt atttatttat tcggccgata
     9781 aatcccaata attagcatct ttaaattcaa tttttggttt tcctaatgat ttccaatctt
     9841 taaatgattt agaatataat ttaatatttt tgatacctgc taatttgaat gcataaaatg
     9901 caagaccaga taaagttcct acactaccac aataagtaat tatttccgaa tcttgaggaa
     9961 tccctctatt ttcaacaaaa cgttttaatt cgtcaacgga tcgcaaaata tgattatcat
    10021 tacccaacat cgtgtaagga atattttttg cgctaggaat atgttcagat aaaaaattaa
    10081 gtcgttctct agaatcaact aataccttat caggattatt ttgtatagat tcaacatatg
    10141 tagcatctgc ataaatagat tgatttaatt taagagaatg ggttgtttct
//
LOCUS       DASVEV010000105         6987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1396143, whole genome shotgun sequence.
ACCESSION   DASVEV010000105 DASVEV010000000
VERSION     DASVEV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396143"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VF222_04935"
     CDS             <1..519
                     /locus_tag="VF222_04935"
                     /inference="COORDINATES: protein motif:HMM:NF021571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2299 family protein"
                     /protein_id="HEX7141803.1"
                     /translation="LKMNNELDVKEVAFNNIIQWLSDIGVIIEKKEVNKIPESKIQFE
                     LLISTGRGYRYPFRITFTNLLKDVFVVSSNISLTKEDTQSIQAMKKRDREQIFMDLRK
                     LIFPLHVNIETPFPRIFLYREITLDSISTNKQFLIDEVYNFRNAMELVKIRFDELYYS
                     FYPEGSRTDYSN"
     gene            834..1655
                     /locus_tag="VF222_04940"
     CDS             834..1655
                     /locus_tag="VF222_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX7141804.1"
                     /translation="MRNKISIINNLVNHQMFNYIITVVILLQAIVLAFETFSEFNNYI
                     SLFEYINAFVLSIFIIEAILKMVALYPHPYNYFKNGWNILDFFIIVVSLLPLTGGFTT
                     VARLIRLLRVTRLTNRSKEMSVMVMTIIKSLPSMINIFLLLSLLFFIYGIAGYHLFST
                     IDSEHWGTLPKSMITLFKILTLEGWVEIMAPVTDVNPLNGIFFVSFIIIGTFIVINIF
                     VAIIVKKSEEAYKHIQQEFSHSVTQNEILEELKEIKRMMENLEKRIPKDKEKNTT"
     gene            complement(2023..2760)
                     /locus_tag="VF222_04945"
     CDS             complement(2023..2760)
                     /locus_tag="VF222_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141805.1"
                     /translation="MDTDALEIFNTLLLSVTVSSAFLIGAILSIFIHYPERIRADLAA
                     FSAGIFFATIAFSLIDEAIREGSFITMVVGFVVGAVVFSVINRILQTRYKSKTKNKNT
                     SSSKTVIIGTFLDSFPETIFIGVIIALNLPELMSAVFALFLGNLTATMEGAKRMVDDS
                     KHNAKIFKQWLYVFVIVAIGGPLGWYLEKPLSPDQLSMIISFAAGALMAFITEELIPQ
                     AYKRVELHIGLSSSFGFIIALALFHYM"
     gene            complement(3335..4276)
                     /locus_tag="VF222_04950"
     CDS             complement(3335..4276)
                     /locus_tag="VF222_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141806.1"
                     /translation="MGPNFNKKNNSSTYENEIKNVGDSETYKNNENPIIISPKKSKGS
                     RMTIKPSLMHQEGSFSTKVDHLFESVKEKTSDYFDKVKIKQHDINSFAAGAAAANVQE
                     ANEVHNQQEFVIPVLEEKYTLSKNTVLEDIKIEKQWKTHNEKIQVPVSYEKLLVNDKE
                     LDSFSKENIFTQIKDKILDFVYVEGSTNENKIENEVNEETEHSEQEKPMEKYHQHSKN
                     EKQIQQVRGERVSLFDEEKANFTNDKNNNTIENDNTQKVIPLYAEEIIITKRKVKVGE
                     IIITKRKVTEVKKIDLDIVKEKVTVDYANGRKENITE"
     gene            4446..5186
                     /locus_tag="VF222_04955"
     CDS             4446..5186
                     /locus_tag="VF222_04955"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7141807.1"
                     /translation="MESDSKDIPWNEIIKKEAVGINNEDFGKVQEVGSVSILTKKGFL
                     NTEFFDIPKDLVERYDGEKVYFKISEEEARNSSKRNSSITTEKEEEDYSINQSKITTE
                     DENNNLSTHSTSIDSKNNDIVIPLMSEELVITKKEVTDEVTITKEPTKETKTEQVQLM
                     HEEISIERRPVNNLTDTANTSTTKEDIPPIESKTEIKIPLKREEVEVTKKPYVKEEIV
                     VKRKPVTETRQISEEIITEQIVEPGNIE"
     gene            complement(5468..6115)
                     /locus_tag="VF222_04960"
     CDS             complement(5468..6115)
                     /locus_tag="VF222_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7141808.1"
                     /translation="MNPTIDWNDVIKKEARGINDEDLGEVQLVEDNYVLVQRGIINKE
                     KFYIPKDQAESYDGDVLKFKFSEQELSQYQDEPLSTRDVKSNSDITQENIQEEEKTDE
                     ESVPLTEERLDVSKVSQENQATITKKPVTETKTEQVQLMHEEISIERRPPSGQTEAHS
                     PIQSQEDIKIPLKREEVEVTKKPYVKEEAVIKKKAFTDNKEITEDVTSEELDTSR"
     gene            complement(6283..6828)
                     /locus_tag="VF222_04965"
     CDS             complement(6283..6828)
                     /locus_tag="VF222_04965"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEX7141809.1"
                     /translation="MVSPLIPIAFSQEKIIELENKDYDVKIEYPQSWNVREGDIEPGD
                     FNTQIAIFEPQGDAGIHRSDEYNCADVCIVLYIDNSLRESNLAIETYLDQSINSLKDD
                     LNNFEVIYYDTTNTKLDEHQAYKLVYQEENKDKKYTTVQSGVMYDNQVYIIEYKAMED
                     YYEQYLPVAEEMIKSLKLPDK"
BASE COUNT         2460 a          978 c          877 g         2672 t
ORIGIN      
        1 ttaaagatga ataacgaact agatgtaaaa gaagttgctt ttaacaatat aattcaatgg
       61 ttatctgata ttggtgttat aatagaaaaa aaggaggtaa ataaaattcc tgaatcaaaa
      121 atacaatttg aattacttat atctacagga agaggatatc gttatccttt tcgtatcact
      181 tttactaact tactaaagga tgtatttgta gttagttcaa atatttcact tactaaagaa
      241 gatacacaat caatacaagc tatgaaaaaa agagatcgtg aacaaatctt tatggattta
      301 agaaagttaa tttttccctt acatgtaaac atagaaactc cattcccaag aatatttcta
      361 tatcgagaaa ttacccttga ttctatttct actaataaac agtttctaat tgatgaagta
      421 tacaatttta gaaatgctat ggaattagta aaaataagat ttgatgaatt atattattcc
      481 ttttatcctg aaggttcaag aacagattat tcaaattaac ttgtttgaag tagtataagt
      541 ttttcataaa tagtttatta ctacggctat ttttacccca tacaacccaa ttaacttttt
      601 tattaattag tttatagtat aaatcaatta tttttgcaat tttgggaata atctttctct
      661 tagactattg gttaatggaa aatttttata tgtaacatat ttattagtat attggtttct
      721 aaagtttata aaatattagg tctactttac ttaattgtta tattatttat atttcaacag
      781 cgactatatt gtctttgata attatataac aaaaggagac tagactatta aaaatgagaa
      841 ataagattag tataataaac aacttagtta atcatcaaat gttcaattat atcataacag
      901 ttgttatact actacaagcc atagtattgg catttgaaac attttcagaa tttaacaatt
      961 atatttcctt atttgagtac attaatgcat ttgtcttatc tattttcatt attgaagcaa
     1021 tattaaagat ggttgcactt tatccacatc cgtacaatta ttttaagaat gggtggaata
     1081 ttttagattt ctttataatt gtcgtttcac tgctacctct tacaggaggt tttactacgg
     1141 ttgctagatt gatcaggcta ttacgagtta cacgtctcac aaatagatca aaagagatgt
     1201 cagtaatggt tatgacaata atcaaatcct taccaagtat gattaatata tttttacttc
     1261 tatctcttct cttttttatt tatggaattg caggatatca tctattcagt actatagatt
     1321 cagaacattg gggaacatta ccaaagtcca tgataacatt attcaagatt ctaacattag
     1381 aaggatgggt tgagataatg gcaccagtaa cagatgttaa tccattaaat ggaattttct
     1441 ttgtaagttt tattattata ggaacattta ttgttatcaa catatttgtt gctattattg
     1501 taaagaaatc tgaagaagcg tacaaacaca tacaacagga attttcacat tctgtgaccc
     1561 agaatgaaat actagaagag ttaaaagaaa ttaaaagaat gatggaaaat cttgaaaaaa
     1621 gaatacctaa ggacaaagaa aaaaatacta cttaatatgt aaaaataata agaattattt
     1681 tggtagaata tttagaattt accataaaat ttgttattag ttcttagaac taaatgtaaa
     1741 accataagtt aatctaatga aaaataatat aaatgagagt gagaaattat tggagtcaaa
     1801 caccaaaata aataaaattg aagcaaaaat tcatataaat ttcaattcca ttttttttat
     1861 tgaataaagt taataaaata atggtatcta attaaatcaa ttgatgacta atttgtatat
     1921 attatgttaa atcatcattt tagaatttat atatggaatt gcttgttgtc tttaattatt
     1981 ctgtaaatga tttattcaat taaaaatgtt aaagtatatt tgttacatat aatggaataa
     2041 agctaaagct ataataaatc caaaagagga agataatcca atatggagtt ctactcgttt
     2101 atatgcttga ggtataagtt cttcagtaat aaaagccata agtgcaccgg cagcgaagct
     2161 tataatcata gataactggt caggacttaa tggtttttcc aaataccacc ctaatggacc
     2221 gccaatagca actataacaa aaacatataa ccattgttta aaaattttag cgttatgttt
     2281 tgagtcatca accattcgtt ttgctccttc catagttgct gttaaattac ctagaaatag
     2341 tgcaaaaact gcagacatta attccggaag atttaaagct atgatgacac caataaatat
     2401 ggtttctgga aagctatcga gaaatgtccc tatgataacg gttttagatg agctagtatt
     2461 cttgtttttg gttttagatt tataccttgt ctgaagtatc ctgttgatca cactgaaaac
     2521 aaccgcacca actacaaaac caactaccat agtaataaaa gagccttctc ttattgcttc
     2581 atctattaat gaaaaagcta ttgttgcaaa aaaaattcca gccgaaaagg cagctaaatc
     2641 cgctctaatt ctttctgggt aatgaataaa aatggaaaga attgcaccaa taagaaaggc
     2701 gctagaaact gttacagaca gtaataacgt attgaaaatt tctaaagcat cagtatccat
     2761 atacatacta tttaaaatta attaccaatt tgaactaata aaaaagtatt aatttcttct
     2821 atcaggttta ctgcttctta atatactatt ttttcttttt gtttatctcc attacaacat
     2881 tcattagata ttttcaataa tctcatattc tgatatttcc tgcgtgctag aacattttta
     2941 ttaaattata tctcaatcga ttataatact taaaattgca tcaacaacta tatctataaa
     3001 aagatagaat atataataaa tttacttatc tataattttt attttctata ctttactcac
     3061 gatcagaatc tcctattttt agtagcttct ttttttgatt ctttcttaat attctcagag
     3121 acatgtgtga gtgtctcttc aatatcattt ttcttatcat tattgtctca tctttatttt
     3181 cataattcat cttaatacta tatcatcaat aacatttaat agagtattac ctaaagttaa
     3241 atattcttta taaaataaat gacacttcat ctgattctat attttttatc acaaaaataa
     3301 acgtagattt atctaaatat agtaatgatc tttgttactc tgtaatattt tcttttctac
     3361 cattagcata atcaaccgtt actttttctt tgactatatc taaatcaatt ttcttaactt
     3421 cggttacctt tcttttagtt attattattt ctccaacttt tacctttctt ttagttatta
     3481 ttatttcttc agcatacaaa ggaattactt tttgtgtatt gtcattttct attgtattat
     3541 tatttttgtc attggtaaaa ttagcctttt cctcatcgaa taaagatact ctttcacctc
     3601 ttacttgttg tatttgtttt tcattttttg aatgctgatg atatttctcc ataggcttct
     3661 cctgttccga gtgttctgtc tcttcattta cctcgttttc aatcttgttt tcattggtac
     3721 ttccttcaac gtatacaaaa tctagaattt tgtccttaat ctgagtaaaa atattttcct
     3781 ttgaaaatga atcaagctct ttatcattaa ccaataattt ttcatatgat actggtacct
     3841 gtatcttttc attgtgagtt ttccattgtt tttctatttt aatatcttct aatactgtat
     3901 ttttagaaag tgtgtatttt tcttctagaa ctggaataac aaattcctgt tgattatgta
     3961 cttcatttgc ttcttgtaca ttagctgctg ctgctcctgc tgcaaatgaa ttaatatcat
     4021 gttgttttat cttgacctta tcaaagtagt cagaggtctt ttcctttacc gattcaaata
     4081 aatgatctac tttagtagaa aaggatcctt cttgatgcat taaagatggt ttaattgtca
     4141 ttcttgagcc ttttgatttt tttggtgaaa ttataatagg attttcatta tttttatatg
     4201 tttcactatc accaacattt tttatttcgt tttcatatgt ggaagaatta ttctttttat
     4261 taaaatttgg tcccattact aacacttaca taatataact taagtatgtt ctaactttta
     4321 agataaaatt gaaatatatt ttctaaattc atatgctaac catatgattt aacaaatcta
     4381 ttaaataaat agctgaaata cactattata tcaattgata tattatatgt tgaagttaga
     4441 taattatgga gtctgattca aaagacatac cttggaatga aattataaaa aaagaagcag
     4501 taggaattaa taatgaagat ttcggtaaag tacaagaagt tggttcagtt tccattttaa
     4561 caaaaaaagg ttttcttaat accgaatttt ttgatattcc taaggattta gtggaacgtt
     4621 atgatggcga aaaagtttat ttcaaaataa gtgaagagga agcaagaaat tcctctaaaa
     4681 gaaattcatc aataactact gaaaaagaag aagaggatta ttctattaat caatcaaaaa
     4741 tcacaacaga ggatgaaaat aataatctat ctacccattc tacttctata gatagtaaaa
     4801 ataatgatat tgttattcct cttatgtcag aagaactagt tattacaaaa aaagaagtaa
     4861 cagatgaagt aactattact aaagaaccaa caaaggaaac taaaacagaa caagttcaac
     4921 ttatgcatga agaaataagc attgagagaa gaccagttaa caatttaaca gatactgcta
     4981 atactagtac aacgaaagaa gatatacctc caatagaatc aaaaacggaa ataaaaatac
     5041 cgttaaagag agaagaagta gaagtaacaa aaaaacccta tgttaaagag gaaatagtag
     5101 taaagagaaa accagtaact gaaacacgtc aaataagtga agaaataata actgagcaga
     5161 tagttgaacc aggcaatata gaataataaa tatttaaaat atttatctta aaatatagag
     5221 gtacaattct ataatatgtt attatataaa tgttattata taaatcaatt atttatgtaa
     5281 agatatacct aaataaaaag agaacaaatt acaaatctat ctctacaaaa tattttaata
     5341 caaagtaaac agaaattgaa aaaaagtgaa tactaaaata aaaattaggt aaactatgtt
     5401 tgttaatatt actaaaaata aagataaatg ttaatgtatt atttttccta gtttactaat
     5461 ctataattta tctagaagta tctagttctt cagatgtgac atcctccgtt atttctttat
     5521 tgtcagtaaa agccttcttt tttattacag cctcttcttt aacatagggt ttttttgtta
     5581 cttctacttc ttctctcttt aacggtattt ttatatcttc ttgtgattgt attgggctgt
     5641 gtgcttctgt ttggccactt gggggtcttc tctcaatgct tatttcttca tgcataagtt
     5701 gaacttgttc tgttttagtt tctgtaactg gttttttagt aattgttgct tgattctctt
     5761 gagaaacctt agacacatcc aatctttcct cagttaatgg tacactttct tcgtccgttt
     5821 tttcttcttc ttgaatgttt tcttgagtaa tatcagaatt acttttgaca tcacgtgtag
     5881 atagtggctc atcttgatat tgactcaatt cttgttcaga gaatttgaat tttaatacat
     5941 caccatcata actttctgct tgatcttttg gaatgtagaa cttttcttta ttaattatcc
     6001 ctctttgtac taatacataa ttatcttcaa ctagttgtac ttcacccaaa tcttcatcat
     6061 taatacctct tgcttctttt tttataacat cattccaatc gatagtagga ttcataataa
     6121 atattacact ctataagtta tttaataatt gtagaaattt atatgaattt tatctgctct
     6181 acttatagta aattagcaca ttgattattt taaattttta tttaataatt actctagatg
     6241 gttttactaa aggtcctatt atggaaccag ataataacta aatcatttat ctggcaattt
     6301 caaggattta atcatttcct ctgcaactgg taaatattgt tcatagtaat cttccattgc
     6361 tttatattct atgatataca cttgattatc atacataact ccactttgta ctgttgtata
     6421 ttttttatct ttattttctt cttgataaac cagtttgtat gcttgatgtt catctaactt
     6481 tgtatttgta gtatcataat atatgacctc aaagttatta aggtcatctt ttaacgagtt
     6541 tattgattga tccaaatagg tttctattgc taaattactt tcacgaaggc tattatcaat
     6601 atataataca atacatacat ctgcacaatt atattcatca cttctatgta ttccagcatc
     6661 tccttgaggt tcaaatattg caatttgtgt attaaaatca cctggttcaa tatcaccttc
     6721 cctaacgttc caactttgag gatattcaat tttaacatca taatctttat tttccaattc
     6781 aattattttt tcttgagaaa aagcaatcgg aattagtgga gaaaccataa ttatcagact
     6841 caatataaat agtaaaaaag aaattttcat ttgttgatgt attagagttg agtatttaaa
     6901 ttagcgatta tttaataatt tattgttttg ttaatttttt tgatatcata ttttaattgg
     6961 attcataaga atatcatgaa ctttaat
//
LOCUS       DASVEV010000106         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_35911, whole genome shotgun sequence.
ACCESSION   DASVEV010000106 DASVEV010000000
VERSION     DASVEV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35911"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            590..886
                     /locus_tag="VF222_04970"
     CDS             590..886
                     /locus_tag="VF222_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141810.1"
                     /translation="MQNSFPMREWHVKNMEKTIIKHVKGLSPDATKFERRMYKRYGGI
                     HKVQMRIKYDIKHGVNNEEVILFLQKIRTGMEFSDLLKSDGSLDRLNEIESYFI"
     gene            974..1180
                     /locus_tag="VF222_04975"
     CDS             974..1180
                     /locus_tag="VF222_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141811.1"
                     /translation="MVCKGICLRHKAIKPSNIGRYLAGQKRCQVCQVFIKWGGFWCPC
                     CGYRLRTRPRNKKYKSRLKLEQEL"
     gene            complement(1947..2270)
                     /locus_tag="VF222_04980"
     CDS             complement(1947..2270)
                     /locus_tag="VF222_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX7141812.1"
                     /translation="MSEKQPFFTAEISIVPITTNDNTSMSKEIALVYKAINQIQGLKT
                     ILTAMGTQIESNKLDNILEAIKISHTTLRNIGIERIISSIRIDERPDKNNTLDGKINS
                     VKIKI"
     gene            3036..3476
                     /locus_tag="VF222_04985"
     CDS             3036..3476
                     /locus_tag="VF222_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7141813.1"
                     /translation="MNQQTKSVYNFAPLPLRIIAGIGFMMHGLPKLLDIPNTQNSFVN
                     MGLPPELAIIIGLLEFIGGLAILLGIFTRIAAGLLAIDMIGAILSVKLSKGFIGGYEL
                     DLLYLAIMISLMLTGPGNFSIEKNVLKREIFPKASKDSINEKKN"
     gene            4160..>4750
                     /locus_tag="VF222_04990"
     CDS             4160..>4750
                     /locus_tag="VF222_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141814.1"
                     /translation="MKFKNNSEKDGIEDDLEKLLDIVIKLVSGLDNKSKVALKELLVE
                     IEMIYTKIIDILLPFYELSNNTIFQNEFRSRYMNYNEIFQKDLKYIDKSSSIIKTKVE
                     YLIENHGWRSDKIEKVMQVFGKKEDNSKKEELLKELEKMIDNYYSNNKKPFYKTSEIL
                     LKQLNKELDDVNSSLDRGDITTARMKLHLFFEESEFS"
BASE COUNT         1843 a          574 c          599 g         1734 t
ORIGIN      
        1 aattaattca tctacattga ccttaatatt agatacttta ttaataaaat gaaaatagat
       61 ctttgcctga taaataaaat tgtatacaac atcgtgattt atataacatt attttatagt
      121 atattaagaa aattaaatat cactaaaatt ataatttata aatatctatt tactgtgatt
      181 ttcctttaca gtaaattaat attttaatca ttatgaagtt atttgctttt acaatttagt
      241 aaactaaatt ctaaactcta tacgatacaa tttgtattct ttcttcttta taataaaatt
      301 aatttatctg agaatattta ttatttttta tttttcctca agatcaggtt tgacaataaa
      361 tctcaatctc ccatcaacca agttgtatat cttattttaa tttgtattaa ggaatagtta
      421 tttttatttc taatagtaag atttttttat tcatattctt ttatcagttg actaaaatta
      481 ggtcatatgc ttatttaata taaatacata atactatttt gttaaaatat gcataaatta
      541 ataggttgta ttatatacgg ctttattgta ttgtacattg atttttttga tgcaaaatag
      601 ttttcctatg agagaatggc atgtaaaaaa catggaaaaa acaataataa aacatgtaaa
      661 gggactatca cctgatgcta ctaaatttga acgaaggatg tataaacgat atggtggaat
      721 acataaagtt caaatgagaa taaaatatga cattaagcat ggcgtaaaca atgaagaggt
      781 tattttgttt ttacaaaaga taagaactgg aatggaattt tctgatcttc ttaaaagtga
      841 cggttcttta gatagactaa atgagattga gtcatacttt atttaatcaa attcatgtaa
      901 attcaatctt aaattatttt atttaagttt aacataactg attctagatt actaaaacta
      961 ggagtgacta aaaatggtat gtaaaggaat atgtttgcgt cataaagcaa taaagccctc
     1021 aaatattggt cgttacctag ctggacaaaa acgctgccaa gtttgtcaag tttttattaa
     1081 atggggtggt ttttggtgtc cttgttgtgg ttatagatta aggactaggc ccagaaataa
     1141 gaaatataaa tctagattaa aattagagca agaactttaa tagggtatca taagaataaa
     1201 taaataaatg tataaataaa taaataaata aattaagttt ctttataatt ctatataaat
     1261 ttatcctgct ttaccttata gtttaaagtt taagtttccc catactatat tctgtatgaa
     1321 ctttgactat ttcaaaaaaa ataataataa tgctttatta tagatactat aatacaaata
     1381 taaaaataat tcaaaattat tacatatact atgcaataac ttattcaata actattaata
     1441 atatcgctat agacaattat aatataaaat aataagttgt atactagaga tgactattaa
     1501 actatactta atgaaggata aatagatcaa tagttatatt ttttataata taagtctaat
     1561 aatattaata ccgtaattag aaatatcata tttttctatc aaagaatcaa tacccttcta
     1621 taaaataatc atctatataa ggtgagacat aaatattttc tattaaattg tctaaattta
     1681 tttatttgtt tccagggata taaattggca agcctttttc attatttaaa aaattttttc
     1741 agaaacacct tatagaaata ctacttaagc cttaaaatcg cgaagctaca actacaccaa
     1801 tactatcaat tacaatagga ttggcttttt ttgtaattgt attataaaat aatatataaa
     1861 aatattttta aacaaaaaat atttagaatc tttattttta taataacaaa taatataata
     1921 aaaattcttt catcttatgt tgttttttaa atcttaattt ttacagaatt tatctttccg
     1981 tcaagagtat tgttcttatc cggtctttca tcaatcctaa ttgaagatat tatcctttca
     2041 attccgatat ttcgaagtgt tgtatgggaa attttaatgg cttctagaat attgtccaac
     2101 ttgtttgatt caatttgagt acccattgca gtaagaattg ttttcaagcc ttgaatttga
     2161 ttaatagcct tatatactaa tgctatttct ttactcatac ttgtattatc attagttgta
     2221 ataggtacta tactgatttc tgcagtaaag aatggttgtt tttcactcat actattcaac
     2281 tagatatata ttattattgt tattaaattt tctttaatac tacttagatt atattgaaat
     2341 aaaacattta atattattat atgaattttt ttttatgcta aactttcata gatctaaaat
     2401 aaatcaattg agtaacattt aacacaataa tagtctattt tatgatatta ttttactatt
     2461 aacattggaa ttttttcaat acaaaaataa ttgaagggaa gtttgtaaat ttatgactca
     2521 acaaatggac aattaactac tactactact actcattcaa cttctgttgt taaacgacct
     2581 tactttgagt attatgttat ttcttcaagg gaggtagaaa ttgtgcctat taaaaaggaa
     2641 agacaaagct aaacattcag tttgagtctt aactataatt gttttgaaaa atgggtgtta
     2701 aaggggagag agagaacctc caacacccgg ggaagacgac aaatacacaa atcaggttat
     2761 tggtaaatac catccaaaat agtgagttaa aaaacttgtt atccgacgtt atttgagagc
     2821 catttcgaac caactaagag tttacagaac ctattttaca atgaaattat aataatttta
     2881 tttatattat acattgtcac atacattttt aaccttcccc taaccatcac ctggttgatt
     2941 tcttgtaatt tacatcatta gtactatata ccagataggt ctcttttatt ttgaaatgtc
     3001 taccttaaaa tatataggaa tttaaaataa tatccttgaa tcaacaaacg aaaagcgttt
     3061 acaattttgc acctctacca ttgaggataa tagcaggaat tggatttatg atgcatggcc
     3121 tgcctaaatt gcttgacata ccaaatactc aaaattcatt tgtgaatatg ggattaccac
     3181 cagaattagc aatcattata ggattattag aattcatagg cggtttggct attctactag
     3241 gtatctttac aagaattgca gcaggattac tagcaataga tatgataggt gctattctat
     3301 cagtaaaatt atcaaaagga tttattggcg gatatgaatt agatttgtta taccttgcta
     3361 taatgataag tttgatgtta acaggccctg gtaatttctc aattgagaaa aatgttctta
     3421 aaagagaaat ctttccaaag gcatccaaag attcaataaa tgaaaagaaa aattagatag
     3481 ataaaatcaa agtattttat tttatttttg taagacagta attcatgtag aaaagagatg
     3541 taaatctaat aatacataga tatctagatt cattcattac aaattaaaag atcaatgaaa
     3601 tatagtcaaa tccgctacca atagtaaatg gatggcaatc atttgtacag ccacaatagt
     3661 tgatatacat aaagcaacag caatatgctt tgataattca ataaccctaa ttttttaaaa
     3721 acatttctct aaatattatt attctataaa gtatatattt atagtaatat aaaaattgtc
     3781 attattaaag aaaaaattcc tattggtaca aatatgatgt ttcatagagc caaaatacta
     3841 tagctaatac cttctacata acactagtga agaaataaca atcataatat gttaaattaa
     3901 aatagttatt gtacatacta taatgcatta caaggttcgt cgtctacatt tcacatatag
     3961 agcaatcata tatttacaat ctggcaacta tccaaaataa caacagatac aatgaagatc
     4021 gtgatgtgtg aaaagatatt atcatcttct ttttcttctt tttaccttat tcagaggtat
     4081 aagacacata tttacagcaa tagattctat actaatattc aaaaacttat taaattcctt
     4141 caggtattat tataatatat tgaagtttaa aaataattca gagaaagacg gtattgaaga
     4201 tgatttagaa aagttattgg atattgtaat caaattagtt tcaggtttag ataataaatc
     4261 aaaagtagca ttaaaggagt tattagtaga aatagaaatg atatatacaa agatcattga
     4321 tatactctta ccattttatg aacttagtaa taatacaata tttcaaaatg agtttagatc
     4381 tagatatatg aattataatg aaatttttca aaaagatctt aaatatatag ataaaagttc
     4441 cagtataatt aaaactaagg tagaatatct aattgaaaat cacggttgga gatccgataa
     4501 aattgaaaaa gttatgcaag tttttggaaa aaaagaagat aattctaaaa aagaggaact
     4561 tttaaaagaa ttggaaaaaa tgattgataa ttattactct aataataaaa aaccttttta
     4621 taaaacatcc gagatactgc taaagcaatt aaataaggaa ttagatgatg ttaattcttc
     4681 gctagataga ggagatataa ctactgctcg tatgaaacta cacttatttt ttgaagaatc
     4741 tgaatttagc
//
LOCUS       DASVEV010000107         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_231085, whole genome shotgun sequence.
ACCESSION   DASVEV010000107 DASVEV010000000
VERSION     DASVEV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231085"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1305
                     /locus_tag="VF222_04995"
     CDS             82..1305
                     /locus_tag="VF222_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7141815.1"
                     /translation="MSDDILDNIFENAVKGNSLIKTRKILTIDYIPHKLQFRDEETTT
                     IAQTLSVSLKGARPSNLLLFGKPGTGKTAVVKNVINKFKEKSTELGVEVIVVFINAKI
                     ANTAYKVLYEIAEEMNINKTDKKNQVYFTGLSLGEATNRILDFIKKRQLHVIFVIDEI
                     DSLVDKNGDDILYNFTRANEQFQEVGFISLIGISNSLTFKDKLDPRVRSSLSEEEIVF
                     NPYKIHQLQKILSERVKLAFHDDVVTEGTINLCAAIAGKDNGDARKAIDLLRVAAEIA
                     EIDKDTKVYEKYIRLAQHKIEKDTNFEIIKNSTTHTKLVLLAIAKSKNGNTGEVYEIY
                     SSLCKHIDNESLTQRRITQIINELDQLGLISTNIINQGRYGRSQKIKITIPLSTLKEA
                     LFDDHLLSTITTTID"
     gene            complement(1295..2887)
                     /locus_tag="VF222_05000"
     CDS             complement(1295..2887)
                     /locus_tag="VF222_05000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HEX7141816.1"
                     /translation="MSFQKEISNALSYITAQGYQIHPEAFNILKNLDSNIMEIIQKVV
                     KFKKSHNDTNIILIEDIGNFCTIKISNELDSLTTSDSKFQDNTIKTINEIQEFKIIST
                     QKITTDKSNYRESFKIIYDSADYIDPQEGINGYISLFKSRFHQSLKILSTRPESKHIH
                     KISSIKRKIKQKNNNYLENNLSLNGNTSIIAGLVMSKRSKKNNIELAIDDLTGIINTI
                     ALNEKTIKLVSKLTLDQMVMLEIENKGINYVIKKVISPDIPDHIPNKGKSDEYVVLIS
                     DLHIGSKYFKENEFLKFLNWLNSSSSENEIVSKIKFLCIGGDLIDGVGIFPNQEKELN
                     DSTIYQQMCHLTNLLEQIPKKIQVFVIPGNHDLGRRALPQPAIPKKDAEILYTWKNFT
                     MLGNPSYIELNGVKILMYHGQGLDDIIATIPGLSYSNPAEAMKTLLKARHLSPIYGQR
                     TPISPEKDDMMVITEIPDIFHSGHVHVMDVQNYRGTLIVNSGTWQDQTPFQQTMGIMP
                     TSGIAIAVNLSTLRPFQIDFSQ"
     gene            2984..>3477
                     /locus_tag="VF222_05005"
     CDS             2984..>3477
                     /locus_tag="VF222_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX7141817.1"
                     /translation="MKKRLYYLAVGMLVFLISYSIGAKINIEKEQSSLIKEQFETKIQ
                     GIDEVGIFLNNVLIALSMFIPGIGIGIGIFSGFGTGLVFNAMALENTTLQNISPLAIL
                     VTPFGIMEIFSYGLAISRSGMLIYDLFKKKSWKLLLKPTIIEIVIVVSILIVAAFIEW
                     NMIK"
BASE COUNT         1250 a          465 c          513 g         1249 t
ORIGIN      
        1 aatataataa catgttaata taaatttgaa atgcaataga aagtaagggg taaggggata
       61 attttatatg gtgagttatt tttgagcgat gatattttag ataatatatt tgaaaatgca
      121 gtaaaaggaa actcgttaat caaaactagg aaaatcttaa caatagacta tataccacat
      181 aaattacaat ttcgtgatga agagacaact acaatagctc aaactttatc tgtatcgtta
      241 aaaggagcaa gaccatcaaa tttactgcta tttggtaaac cgggaacagg aaaaactgca
      301 gtagttaaaa atgtaattaa caagtttaaa gaaaaatcaa cagaacttgg ggtcgaggtc
      361 atcgtggttt ttattaatgc taaaatagct aatacagcat ataaagttct ttatgaaata
      421 gctgaagaaa tgaacataaa taaaacggat aaaaaaaatc aagtttattt tactggtcta
      481 tcgcttggag aagcaacaaa tagaattctt gatttcatta aaaaaagaca attacatgta
      541 atttttgtta tagacgaaat cgattcactt gttgacaaaa atggtgatga tatactttat
      601 aactttactc gagcaaatga acaatttcaa gaagttggtt ttattagtct cattggaata
      661 tcaaatagct taacattcaa ggataaattg gatcctagag tgagaagtag cttaagtgaa
      721 gaagagattg tatttaatcc ttataaaatc catcaattac aaaagatatt atctgaacga
      781 gttaagttag cctttcatga tgatgttgtc actgaaggaa caataaattt atgtgcagca
      841 attgcaggaa aagataatgg tgacgctaga aaagcaattg atttgcttcg cgtagcagct
      901 gaaatagcag aaatagataa ggatactaaa gtatatgaaa aatatattcg tctagcacaa
      961 cataaaatag aaaaagatac taattttgaa ataattaaaa attctactac tcataccaaa
     1021 cttgttttac tagcaatagc gaaatcgaaa aacggcaata ctggggaagt ttatgaaatt
     1081 tattcctcat tatgtaaaca tatagataat gaatcactta cacaacgtag aataactcaa
     1141 attataaatg aacttgatca acttggttta atttcgacta atatcattaa tcaaggcaga
     1201 tatggtcgct ctcaaaaaat caaaattacc attcccttat ctacacttaa agaagcatta
     1261 tttgatgatc atctactatc tacaataaca acaactattg actaaaatca atttgaaatg
     1321 gacgtaatgt ggaaagatta acagctattg cgattccaga ggtaggcatt attcccatag
     1381 tttgttgaaa tggggtttga tcttgccatg tcccagaatt tacaatcaat gttccacgat
     1441 aattttgtac atccattaca tgtacgtgtc cagaatgaaa tatgtccggt atttcagtaa
     1501 ttaccatcat atcgtccttt tctggtgaga ttggtgtcct ttgaccatat attggagaca
     1561 aatgtcttgc ttttagtaat gttttcatcg cttcagcagg attagaataa cttaatcctg
     1621 gtattgtagc aataatatca tccagtcctt gaccatgata catgagaatt ttaacaccat
     1681 ttaactcaat atagctagga ttacctaaca tagtaaaatt tttccaagtg tataatattt
     1741 ctgcatcctt ttttggtata gcaggttgag gtaaagctct tcttccaaga tcatgatttc
     1801 ctggaataac aaatacctga atcttttttg gaatctgttc taggagatta gttaaatgac
     1861 acatttgttg atatatggta ctatcattta gttctttttc ctgattggga aatattccaa
     1921 ctccatcaat taaatctcct cctatacaga gaaatttaat ttttgaaacg atttcatttt
     1981 ctgacgatga cgaatttaac caatttagga attttaaaaa ttcattttct ttaaaatatt
     2041 tactacctat atgcaaatca gaaattagaa ccacatattc atccgatttt cccttattag
     2101 gtatatgatc aggaatgtca ggggaaataa cttttttaat aacataattt attcctttat
     2161 tttcaatttc caacattacc atttgatcca atgttaattt tgatacaagt tttattgtct
     2221 tttcatttaa agcgattgta tttataattc ctgttaaatc atctatagct aattcaatat
     2281 tattcttttt agatcgttta gacattacca gaccagcaat tatggaagta tttccattta
     2341 acgaaagatt attttctaaa taattgttat ttttttgttt aatttttctt tttatggaag
     2401 atattttatg gatatgttta ctttctggtc ttgtagataa aatttttaaa gactgatgaa
     2461 atctactttt aaataatgaa atatacccat taataccttc ctgtgggtca atataatctg
     2521 cagaatcgta aataatttta aatgattctc tataatttga tttatcggtt gtaatttttt
     2581 gtgtagatat aattttgaat tcttgaattt catttattgt tttgatagta ttatcttgga
     2641 actttgaatc tgaagtagtt aaactatcaa gttcattgga aatctttata gtacaaaaat
     2701 ttccaatatc ttctataaga ataatattag tgtcattgtg gcttttctta aatttaacaa
     2761 ctttctgaat tatttccatt atatttgaat caagattttt taaaatgtta aatgcttcag
     2821 gatgtatttg atatccttgt gcagtaatgt aagacaatgc atttgatatt tctttctgaa
     2881 aagacaacta caaactatta cgatagaatc atcttattta attagattgc tattataagt
     2941 tcaatacaaa aatattataa agttaaataa tatatcataa aaattgaaga aacgtcttta
     3001 ttatttagct gttggtatgt tagtttttct tatttcttat agtattggtg ctaaaataaa
     3061 tattgaaaag gaacaatcat cattaataaa agagcaattt gaaacaaaaa tccaaggaat
     3121 agatgaagtt ggcatttttc tgaataatgt tctaattgcc ttatccatgt ttattccagg
     3181 tattggtata ggaattggaa tattttctgg gtttggtaca ggattagttt ttaatgcaat
     3241 ggctttagaa aatacaacat tgcaaaatat ttccccacta gcaattcttg ttactccatt
     3301 tggtataatg gaaatctttt cttatggtct agcaatctca agaagtggaa tgcttattta
     3361 tgatcttttt aaaaaaaaat cttggaaatt acttcttaaa cccactataa tagaaattgt
     3421 aatagtagta tcaattttaa tagttgcagc attcattgaa tggaatatga taaaaca
//
LOCUS       DASVEV010000108         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1202951, whole genome shotgun sequence.
ACCESSION   DASVEV010000108 DASVEV010000000
VERSION     DASVEV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202951"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF222_05010"
     CDS             <1..281
                     /locus_tag="VF222_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141818.1"
                     /translation="LPNLILEKSLTLKLDDLNSKLALFANDNEFTKLQNEKLNIEKKL
                     NETKENFLPNLILEKSLTLKLDDLNSKLAFLTDNRYCSDLEKHIPDQN"
     gene            913..2172
                     /locus_tag="VF222_05015"
     CDS             913..2172
                     /locus_tag="VF222_05015"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141819.1"
                     /translation="MKENINVVFWNLGNLFDIQPNEISSNLEFTPNKGWNEKVRDIKI
                     QNLTNIIKNTFNNGPDLIGVCEIENAFLAEKLIDELKKSLGRDDYQIAGTNIDGKLKP
                     YHKGPDLRGIDTCLIYSNKIFEQLDGHGYMINFRFPTRDIFYAKLRVLKNNAKLNVLV
                     NHWPSRRGRDDYFELMDTEYSRYMAAEHCGRIVDSILKYPKMEIEKFPNNVDSIQEGK
                     TVLEKLEERWNINILVMGDFNDEPFDKSIMKYLYATPDKNLLRKWNRIFRLAKEDFKN
                     RDKTYKQIYLEQPSVLFNCMWNLYPNGSLYFRKTNSFNLFDQFIISRGLLYGKQGLKM
                     NLASVNINTQGMTLGENLSESKFEQENNYDERLIPSEFKQRPMSFEFDRTKNDINNSS
                     IIGNENKSRQPTGYSDHFPVTCTIDIL"
BASE COUNT          984 a          326 c          328 g          848 t
ORIGIN      
        1 ttctacccaa tctgatacta gaaaaatctt taacattaaa actagatgat cttaattcta
       61 agcttgcatt atttgctaat gataatgaat ttacaaagct acaaaatgaa aaacttaaca
      121 ttgaaaagaa actgaatgaa actaaagaaa attttctacc caatctgata ctagaaaaat
      181 ctttaacatt aaaactagat gatcttaatt ctaagcttgc attcttgaca gacaatagat
      241 attgtagcga tcttgaaaag catataccag atcaaaacta attcaaaata attttttgat
      301 taatttaaca ttattgttat tattcattct aatttctaaa ctaactatat ataaaaaaat
      361 taaagtagta tatatttcgt taagcagcat aaaatttgaa aatatctata aagttaccaa
      421 attaaattaa tacaagttat taatctatta cttaatattt ttatgaatag attaacctaa
      481 tccatcaatt tttttggata atccattaac agataatcgt acctaaaaat attacttttt
      541 taccacccta attcaataag tttttaaatt aaactgcttt tactattgat tgctactata
      601 gataatagtt tgtcacaacg gattcacggg tgttattgta gcctctaaca cccaaaaatc
      661 aataatattt ttatttttta ttgtgcatct atattattac acatctatac catctaagat
      721 gatgtaaaca tagtgggtgt tataaggcaa atctaacatc catttttcaa aataccaact
      781 gatttccaac aactgattta aacaacggat gattcaacag aacacaaaat tatatactat
      841 tataaaaata aaacttcaat cttgaaataa tgcctgtaaa ttttatatat ttaataaaat
      901 cttattatta aattgaagga aaatattaac gtcgttttct ggaatctagg aaatttattt
      961 gatattcaac ctaatgaaat ctcatcaaat cttgaattta ctccaaataa aggttggaat
     1021 gaaaaggttc gtgatataaa gatacaaaat ttaacaaata taattaaaaa tactttcaat
     1081 aacggtcccg atttgatagg cgtatgtgaa attgaaaatg cttttttagc tgaaaaacta
     1141 atagatgaat taaaaaaatc attgggtaga gatgactacc aaatagctgg aacaaacata
     1201 gatggtaaat taaaaccata tcataaaggg ccagacctga gagggattga tacatgttta
     1261 atttattcta ataagatctt tgagcagctt gatggtcatg gatatatgat aaattttaga
     1321 tttcctacac gagatatttt ttatgcaaag cttagagtat taaaaaataa tgctaaacta
     1381 aatgttttag taaatcattg gccatcaaga agaggcagag atgattattt cgaacttatg
     1441 gatacagaat attctagata tatggcagca gaacattgtg gaagaatagt tgactctatt
     1501 ttaaaatatc cgaaaatgga aatagagaaa ttcccaaata atgttgattc aattcaggaa
     1561 ggaaaaacag tattagaaaa acttgaagaa agatggaaca taaatattct agtcatgggt
     1621 gattttaatg atgagccatt tgataaaagt attatgaaat atctgtatgc aacaccagac
     1681 aaaaatttac tacgtaaatg gaatagaatc tttaggttag caaaagaaga ttttaaaaat
     1741 agagataaaa cgtataaaca aatttactta gaacaaccat cagttttatt taactgtatg
     1801 tggaatcttt atcctaacgg aagtttatat tttcggaaaa caaattcatt taatctattt
     1861 gaccaattta taatatcgcg aggcttatta tatggaaaac agggattaaa aatgaacctc
     1921 gcctctgtta atataaatac tcaaggtatg acattaggtg agaatttatc tgaaagcaaa
     1981 tttgaacaag agaataatta tgatgaaaga ttgattccat cggaatttaa acaaagaccc
     2041 atgtcttttg aatttgatag aacaaaaaat gatattaata atagcagtat aatcggaaat
     2101 gaaaataaat caagacagcc aacgggatat agtgatcatt ttcctgtaac ttgtacaatt
     2161 gatatattat agaacattat tttttattat attcattgaa cctttaaggg aagtagtatt
     2221 aacatttatt tttttcacaa tttaattgca ttatatttta tattcatgat attgtatatt
     2281 gtttaggaat tacaataatt gaaaatgcta aattttaaaa tttattccct tttctgtcag
     2341 atgtttagtt aaagcgtcca tataactttg attttccatt tcaaggctaa ggaatactcc
     2401 tgccattcct gtaggtatac tagaacttaa tctatcatgc actacttcga ctatatttat
     2461 tcctaaatca gctatactat ccaata
//
LOCUS       DASVEV010000109         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1009149, whole genome shotgun sequence.
ACCESSION   DASVEV010000109 DASVEV010000000
VERSION     DASVEV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009149"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1128
                     /locus_tag="VF222_05020"
     CDS             463..1128
                     /locus_tag="VF222_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141820.1"
                     /translation="MSGIELAAELGYGVPWLKLILTIALVIATAFFIYVLLSTKFNHV
                     ALKGFDKAKYELNKYHAERYWGIFVAGILIWFWVLGYPWMPPVAFEKGLNETQDVHIV
                     KITAGQWYWKIEDGGYYNNNNNNDQSKTDLASNQPREIQVKAGETVKFVAHSQDVNHG
                     FSILSSSNQMDVPLMQMQVVPGYENIFYYTFKEPGVYTIRCLEYCGWSHPYMVSSITI
                     SSV"
     gene            1131..2867
                     /locus_tag="VF222_05025"
     CDS             1131..2867
                     /locus_tag="VF222_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7141821.1"
                     /translation="MVKIKKDSTILEEYDHEHGPTPLYKISKQLADLTKMFIISSVTF
                     FLIAGSLAIIMRMIQSDLNIMGNQQQTMGLFYAAVTAHGQIMFFGFASMMTVGLSYYL
                     VSKFAKKPLYSFKIAIISYVMLNSGNILLIVSAIMFFGGGWYNLLPLAFHPQNEGWTI
                     FSSILFLLADTLIAIGLTIFCINIVATVLKGKIAAGVQKNESEDNSFFKAPVDLNDSG
                     RVDLLPMENLSPAIRWVSILGISSWFPRKYRKKVPAVSIVVVGIFVNAVALLSGSIGF
                     FTQLAIGFTYLLNPNFEPNWLFAKDAWWFFGHPIVYFTLFSFLAAAYYYIPKFTKKTV
                     PYDKWAYRPWPFYFIFTLMVFSHHSYMDMPNPVFVQIIATIASQAIIFPSGLTIMTIM
                     MYILRSNIRWNISSFFIVSGIIGWVYGGFTGAETGWWGTDVYLHNTLNIVGHIHLVLL
                     MGSILLGIGLIYAIIADLTKKKLGNILGLVHLLTTLIGGFGLALMFTYLGFLGFIRRE
                     AVIPDQFSWALPWLLFFALTVGIGQIIFAFNLSKTLIRKQKPEEYEIVKTQEKRSIEE
                     NHTITSDDVHKL"
     gene            2897..3616
                     /locus_tag="VF222_05030"
     CDS             2897..3616
                     /locus_tag="VF222_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7141822.1"
                     /translation="MNLLFKNLKKNSRSIVTITILAFLLIISFQEWILVLLENDLGYH
                     MIFEHAIFFSIGYLSVNASELLIKYVKINVDKKKDKKNYEIVLSKFIQYWTLFLSTIF
                     FNLNKKWIILFIFIVILMLIWHIPDIFDYATFNPNIHILQHFSFIIVGILLFLSIRLS
                     YESNSIFYLLSSIGMMVLSGLILATTNDIIYTAYSIDSHHISGNYMFILTILIGLVVF
                     PFYLIQRTLFHIRQSTKNQEK"
BASE COUNT         1457 a          545 c          656 g         1462 t
ORIGIN      
        1 aaagcaacac tattaataaa aatggcagat aaagcagata ttgctaaaac tattcctact
       61 ccaaatataa cctgagatag aatgttattc aattatacta taagatcata gcaatattta
      121 aaaagatttc taatatatag acaaataata aaatatcaag taaatttttt tcttatttca
      181 gttttgatga ctatattgtg gaaaaattta ccgaaggaga tgataaaatt aaagcgaata
      241 gctcctaaat aatacaaata atttattcaa ctttattcct agctgtagat ttatcattaa
      301 cttaagagga gcctctatat gcaaaggcaa ttgactgaat aatatctata aagaatttaa
      361 tttcattgat tatattattt atcattgttt aaattttatt tgtttttatt aggaagataa
      421 aaaaactttt tatattttga aataactatt ataataattt taatgagtgg catagagctt
      481 gcagctgaat tgggatatgg agttccatgg ttaaaactca ttttaactat agcactagta
      541 attgctacgg ctttttttat ttatgtttta ctttctacaa aattcaatca tgtcgcattg
      601 aagggttttg ataaagcaaa atatgaactt aataaatatc atgcagaaag atattgggga
      661 atttttgttg cagggatatt gatttggttt tgggtgttag gatatccttg gatgccacct
      721 gttgcgtttg aaaagggatt aaatgaaact caagatgttc atattgtaaa aataacagct
      781 ggtcagtggt attggaaaat agaggatggt gggtattata ataataataa taataatgat
      841 caaagtaaaa cagatcttgc ttcaaatcaa cctagagaaa tacaagtgaa agcgggagaa
      901 actgtcaaat ttgtagctca ttcacaagat gtcaaccatg gttttagtat tttgtctagt
      961 tcaaaccaga tggatgtccc acttatgcag atgcaagtag ttccagggta tgaaaacata
     1021 ttctactaca cctttaaaga acctggtgtt tatacaatac gatgtctaga atactgtgga
     1081 tggagccatc catatatggt atcaagtata acgattagct ctgtgtaaat atggtcaaaa
     1141 taaaaaaaga cagcaccatt ttagaagagt acgatcatga acatggtcct actcccttat
     1201 acaaaatctc caagcaactt gctgatctca caaagatgtt tatcatatca tcagtaacat
     1261 tttttcttat tgcaggaagt ttggcaataa taatgagaat gattcaatca gatctcaata
     1321 ttatgggtaa ccaacaacag acaatgggtc ttttttatgc agcagttaca gcccacggac
     1381 aaataatgtt tttcggtttt gcatcaatga tgacagttgg attgagttat tatttagtga
     1441 gtaaatttgc taaaaagcca ctttatagtt ttaaaatagc tataatttca tatgttatgc
     1501 ttaattcagg gaacatatta ttgatagttt cggcaataat gttttttgga ggtggatggt
     1561 acaacctttt acctctagct tttcatccac aaaatgaggg atggactatt ttttcctcaa
     1621 tattgtttct tttagcagac actctcatcg ctattggtct aactatattt tgcataaata
     1681 tagttgcaac tgttttaaaa ggcaagatag cagcaggtgt tcaaaaaaac gaatcagaag
     1741 ataatagttt ttttaaagct ccagtagacc ttaatgattc aggcagagtc gatctcttac
     1801 ctatggaaaa tctatctcct gctattagat gggtatcgat tctaggtata agttcctggt
     1861 ttcctagaaa atatagaaag aaagttccag cagtatctat tgttgtggta ggaatattcg
     1921 ttaatgcagt agcactcctt agcggaagta tagggttttt cactcagctt gcgattggtt
     1981 tcacatatct cttaaatcct aattttgaac caaattggct atttgcaaaa gatgcatggt
     2041 ggttctttgg tcatcccatt gtatatttta ctctattttc gtttcttgcg gctgcttatt
     2101 actatatacc aaaatttaca aaaaaaactg ttccctatga caaatgggct tatcgcccct
     2161 ggccttttta ttttatattt actctaatgg ttttttcaca tcattcctat atggatatgc
     2221 caaatcctgt tttcgtccaa ataattgcta cgattgcgag tcaggcgata atttttccat
     2281 caggtttgac tatcatgact attatgatgt atatccttag atcaaatata agatggaata
     2341 tttcctcttt ttttatagta tcaggaatta ttggatgggt ttatggtgga tttactggtg
     2401 cagaaacagg ctggtggggt acagatgtat atttacacaa tactttgaat atagttggcc
     2461 acatacattt agttttgcta atgggtagta ttctattagg aataggattg atttatgcga
     2521 taatagcaga tttgaccaaa aaaaaattag ggaatattct tggtttagta catcttttaa
     2581 ctacgttaat aggaggattt ggacttgcat taatgttcac ttatctagga tttttaggat
     2641 tcattagaag agaggctgtt atacctgacc aattctcatg ggctctgcca tggcttttgt
     2701 tttttgcatt aacagtagga ataggccaaa ttatctttgc atttaacttg agtaaaacac
     2761 taattagaaa acagaaacca gaggaatatg aaattgtaaa aacacaagaa aaaagatcaa
     2821 tagaagagaa tcacactatt actagtgatg atgttcacaa attataaaat aagatatatg
     2881 gtaatgatga aatcaattga atcttttatt taaaaattta aaaaaaaatt caagatcaat
     2941 tgtcaccata acaatattgg cttttctatt aataatatct tttcaagaat ggattcttgt
     3001 acttttagaa aatgatttag gatatcatat gatatttgaa catgcaatat ttttttcaat
     3061 aggatatctc tctgttaatg cttcagaatt actaattaaa tatgtcaaga taaatgtgga
     3121 taaaaaaaag gataaaaaaa attatgaaat tgtactctca aaatttattc aatattggac
     3181 actttttcta agtacaatat tttttaactt aaataaaaaa tggataattt tatttatttt
     3241 tatagtaatt cttatgttaa tatggcatat tccagatatc tttgattatg caacttttaa
     3301 tccaaatatt catatattac agcatttttc atttattatt gtaggaattt tattatttct
     3361 ctctatcaga ctctcttatg aatcaaattc tattttttat ttattatcat caataggaat
     3421 gatggtttta tcgggattga tattggctac taccaatgat attatttata cagcctactc
     3481 tatagatagt catcatatat caggaaacta tatgtttatt ttaacaattc taattggatt
     3541 agttgtattt cctttctatc taatacagag aactcttttt cacattaggc agagcacaaa
     3601 aaatcaggaa aaatgaacct gtaaggataa cttgaacttc acaaataaaa ttttagttaa
     3661 tggattttat attactatac ttgagaatat ctaaactaga taataattag attaataaaa
     3721 aatacctaat ggaagctaga aagaataatt tcaattagat gaatagccta tacaaagacg
     3781 ctagttaatc cgtgattttt agctattaaa agaattgata gtagatggta gatgcctaaa
     3841 actaaaaaga actacatttg atatgaatga aaagataagt aatgtagaaa ttttacacta
     3901 aagaaaaatg taaatgataa taatactata aatcaaaatg tcaaatttat ctttcaatca
     3961 acaaaagaac caataatggt atttgctgat aaaagagaga gagaatttac caatgattag
     4021 taatttgata aagactttaa tgaagagagg attgaccttt acatatcata atatttctag
     4081 aatagttagt tctttagtat tttgatattg tgtaatagtt
//
LOCUS       DASVEV010000110         7634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_38084, whole genome shotgun sequence.
ACCESSION   DASVEV010000110 DASVEV010000000
VERSION     DASVEV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38084"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF222_05035"
     CDS             <1..259
                     /locus_tag="VF222_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141823.1"
                     /translation="TVTGDVAQAQTFNAVSPPPLAEATQAETGDGKDGDGKDGDGKDG
                     DGNDGDGNDGDGNDGDGNDGDGNDGQGGGNDNNNDGQGGGN"
     gene            complement(575..1066)
                     /gene="leuD"
                     /locus_tag="VF222_05040"
     CDS             complement(575..1066)
                     /gene="leuD"
                     /locus_tag="VF222_05040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019913.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7141824.1"
                     /translation="MTILKGKIHKYNKNNIDTDVIIPGPYLKIHDHKELGKHAMEGLD
                     PNFSEKICEGDFLLTGKNFGCGSSREHAPIALHEVGIRAILSPSFARIFYRNAVDGGY
                     LLPIEIDPILIEKISDKDEIEIDLNTNTIKNITSSETFNIKPFPEIISKIVKAGGLLY
                     FKE"
     gene            complement(1063..2337)
                     /locus_tag="VF222_05045"
     CDS             complement(1063..2337)
                     /locus_tag="VF222_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7141825.1"
                     /translation="MTITEKILARASNKNSVTPDDVVIANIDKVMVHDVSGPGVIKVF
                     SEWEKKGINLDHIWNPDRVWISEDHFVPASDRISANNITLLSNWTKKYKIRKHFKYGL
                     GQYGICHTLSHEEGLVLPGEIYVGGDSHTNTTGAMGSLAVGLGHTDIAYVLMNGKIWF
                     KVPETILFKIKGDKPEYIMAKDIILKIIGDIGTDGANYKAMQFTGETVDKMSMEERLT
                     LTNMSTEAGAKNGIIDPDETTFEYLKTRTDKEFIPVKGDVDAAYSNTFTYEIENLDPV
                     IAKPYSPENICAVGDLEGRIIDKAYIGSCTGAKLEDLRMAARILKGKQVKIRTEVLPA
                     AQSIYLKAIKEGLISIFLEAGAVVGPPTCGACCGAHMGVLAKDEICISTTNRNFPGRM
                     GHVESQTYLASPLVVAAAALNGKITDPRNEMS"
     gene            2426..3190
                     /gene="cofE"
                     /locus_tag="VF222_05050"
     CDS             2426..3190
                     /gene="cofE"
                     /locus_tag="VF222_05050"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX7141826.1"
                     /translation="MIFVKPIKIKTKKKIFDLFDTIISSKCEFTNGDVLVLSSKFVAM
                     SEGSLIDLQKIRPSYRAKKLAKLYQMDPTFVEVVIRESDVIFGGLPGFLLTMRNGILA
                     PNAGIDKSNVPSNNVICLPMDPFISAENLRMEFLVQLGIRVGIVISDSRLMPTRIGTT
                     GVAIGCSGIEPVEDQRGKKDLFGHIMKYTLKATADSLATMGTFVMGESNESVPLVLIR
                     DSNISFTDRRLCWQDVAIDYQTDIYFRGIQNFDSII"
     gene            3281..3799
                     /locus_tag="VF222_05055"
     CDS             3281..3799
                     /locus_tag="VF222_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141827.1"
                     /translation="MSFLKGLQESIKSEDIQFDHLSLLVKIKKDEIIKTLILEGFSKV
                     KFENKKPGQIGNGFTKKLKKPWEIHIRLLYMHQGLIAIQGEVEISRRYIQHIRSVRAP
                     VIYEIESILKKHRIEYKIWNDKVRDYITNIIENHQIILNSPKLPAMPWLLMIIFGSMI
                     GSLHGLKLFGIF"
     gene            3850..4971
                     /locus_tag="VF222_05060"
     CDS             3850..4971
                     /locus_tag="VF222_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7141828.1"
                     /translation="MGIPEKIKELEDEIHKTQINKATEFHIGILKAKIAKLKRELDEN
                     RHGKTVSSGKGSSGYDVRKEGDATVVLIGFPSVGKSTLLNSLTNANSKIASYQFTTLT
                     AIPGMMEYRGAKIQVIDLPGIIKGAASGKGMGKKVLSVARNADLVLIVVDVFQPKHFS
                     ILVKELAEIGIKVNKKPPNVHIHKTDKGGISVSIDQDSQVSESMVREMCRIYGIHNGR
                     ITIRDPNLTTDDIIDALAANRVYPHSLIILNKIDLIDSNTLNAMIPFLGEAVEPVSAN
                     SNLNIEMLKESIFNKLEFIRIYMRPKGKETDYEEPLIVRNGTRVKDICAKLHRNMLRD
                     FRFALVSGKSAKFEGQKVGLDHVLLDQDVLTIIKKINAI"
     gene            complement(4979..5521)
                     /locus_tag="VF222_05065"
     CDS             complement(4979..5521)
                     /locus_tag="VF222_05065"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7141829.1"
                     /translation="MLENKDKKFSLIKDIAIIVIGVVLIWFAVKVVFNVDNPFYVVSS
                     GSMIPVLNVGDILIVKDGNTFNSLKVGDIIVFNRPQGGDRVIVHRILEISDRFGEKVI
                     VTKGDANDGIIPGTDFPIREKDYIGTVAYTVPKIGLVLKYLNPPVNYIIIAGIIGILI
                     YTNIKKKNDEKGKIEGNNPL"
     gene            complement(5668..6072)
                     /locus_tag="VF222_05070"
     CDS             complement(5668..6072)
                     /locus_tag="VF222_05070"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX7141830.1"
                     /translation="MSILLTVHIDGASRGNPGPSGIGIIVFRNNTLILSYGEFIGFNT
                     NLLSEYLALKRALQFAVIIDKEIRIHTDSMTIVQHRKSKERIRRKELKILSREINNLE
                     KKFKKISYKYVPRERNTTVDLVAKKAIDYHIN"
     gene            6445..7017
                     /locus_tag="VF222_05075"
     CDS             6445..7017
                     /locus_tag="VF222_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141831.1"
                     /translation="MPKRRFKPTKPTKKIGKKQLLLPPVMIGIATFAGIIIMQLSPPP
                     QVLGICLKAHNVETFNIYPVVQIFEDDKQKYLPEGMGKEIKDGKECIHLIHTDEIGDR
                     IHIEYIRPIRLTIDDFMQLYSVDNKTITVLNNETGSFKKEVLPLENYNIQYSYFSEHN
                     EFTQISNSTLMPPFTKDMVVKIELKSKMWK"
     gene            7080..>7634
                     /locus_tag="VF222_05080"
     CDS             7080..>7634
                     /locus_tag="VF222_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7141832.1"
                     /translation="MGNKKAVVIKGDGTGPELVKCMTDVLKSCNSTIELIPVEAGSEF
                     WKRNQTNSYITSEVWKLLSESDACFKGPTTTMPDPNAPRSVAVSIRQKFELYANIRPI
                     KTYKTLQLDLDFVCVREATEGLYAGIEFKTSDDSAVAIRKITRKGCERVVKKAFELCK
                     ELDFKKIFVITKRNILKETDGIFVS"
BASE COUNT         2628 a         1233 c         1217 g         2556 t
ORIGIN      
        1 taccgttacc ggtgatgtcg ctcaagctca aacatttaat gcagtaagtc caccaccact
       61 agcagaagca actcaggcag aaacaggtga tggtaaagat ggagatggta aagatggaga
      121 tggtaaagat ggagatggta atgatggaga tggtaatgat ggagatggta atgatggaga
      181 tggtaatgat ggagatggta atgatggaca aggcggcggc aacgacaaca acaatgatgg
      241 acaaggtggc ggtaactaga acaatatatc atccagacca ttattttctt taatttttat
      301 tcttaatttt ttattcgtaa taattatttc tttagctaac gttataactc taacctaacc
      361 tttttttaaa taaatatttc ttctcttatc tccttttgta aattaatttt tgactttttt
      421 cgattactaa aaagtcatag atcatattat tacaaagttt acaaatttac aaacaatgct
      481 gtatttagaa catttataag tttaaattta aacattccaa agcgttagag aaaaagagtt
      541 tatgtgattt tcgcttctta tgccctcttt tgatctattc cttaaaataa agtaaccctc
      601 ctgcctttac tatttttgag attatttcag gaaatggttt gatgttaaat gtttcactgg
      661 atgttatatt ctttattgta ttagtattaa gatcaatttc tatctcgtct ttatctgata
      721 ttttttcaat caaaatagga tcaatttcta taggtaaaag atagcctcca tccactgcat
      781 ttctgtagaa tatcctcgca aaggaaggag aaagtattgc ccgaataccc acttcatgta
      841 atgctatcgg agcatgttct cggctagagc cacaaccaaa attcttccca gttaataaaa
      901 aatctccttc acatattttt tcggaaaaat taggatccaa gccttccatc gcgtgttttc
      961 ctaattcctt atgatcgtgt atttttaaat aaggtccagg aattattacg tctgtatcta
     1021 tattattttt attgtatttg tgaattttac ctttaagaat agtcacgaca tttcattcct
     1081 tggatctgta atttttccat ttagagctgc tgcagcaact actaaaggcg atgctaaata
     1141 tgtttgtgat tcaacgtgtc ccattcttcc tgggaaattt ctgttagtag tactaataca
     1201 aatttcatct tttgcaagta cacccatatg tgcaccacaa caagcaccac acgtaggagg
     1261 accgacaact gcacctgctt ccaaaaatat acttattaat ccttccttaa ttgcttttaa
     1321 atagattgac tgtgcagcag gtaacacttc tgtcctaatt ttaacttgtt ttcctttgag
     1381 gatccttgct gccattctta aatcttctaa tttagcgcca gtacatgatc caatatatgc
     1441 tttatcaatt attcttcctt ctaaatcgcc gacagcacag atattttcgg gtgaatatgg
     1501 cttagctata actggatcga gattttctat ctcatatgta aatgtattac tatacgcggc
     1561 atcgacatct cctttgactg gaataaattc cttgtcagtt cgtgtcttta gatattcaaa
     1621 tgttgtctca tcagggtcaa taattccatt cttagctcca gcttctgtac tcatattagt
     1681 gagggttaat ctttcttcca tagacatttt atccacagtc tctccagtaa attgcatcgc
     1741 tttgtaattt gcaccatcgg taccgatatc acctattatc ttcaatatta tatctttagc
     1801 cattatgtac tccggtttat ctccttttat cttaaaaaga attgtttccg gtactttaaa
     1861 ccatattttt ccattcatca atacatatgc aatatctgta tgacccaatc caacagcaag
     1921 tgatcccatt gcaccagtag tatttgtatg agagtcgcca cctacgtaaa tctcaccagg
     1981 caaaaccagc ccttcttcat gagataaagt atgacaaatt ccatactgac ctaatccata
     2041 tttaaaatgt tttcttattt tgtatttctt agtccagttg gacaataatg taatattatt
     2101 agctgaaatc ctatcagaag caggaacaaa atgatcttcg cttatccata ctcgatctgg
     2161 gttccaaatg tgatcaagat ttataccttt tttttcccat tctgaaaaaa ctttaattac
     2221 accaggacca gacacatcat ggaccataac tttatctata tttgctatga ctacatcatc
     2281 aggagtaaca ctatttttat tggaggctct agctaaaatt ttttcagtaa tagtcattcc
     2341 catttcttta tactaaataa agttaacaat tcgtgaataa aaacgttcta aaaattaata
     2401 acgttctcta ctttaaataa taatattgat atttgtaaaa cctattaaaa taaaaactaa
     2461 aaaaaaaata tttgatctct ttgatactat tatatcctca aagtgtgagt ttacaaatgg
     2521 agacgttctt gtattatcaa gtaaattcgt tgctatgagc gaaggttctt taatagatct
     2581 gcaaaaaatt agacctagct atagagccaa aaagcttgca aaattgtatc aaatggatcc
     2641 aacgtttgta gaggtagtaa ttagagaatc agatgtaatt tttggagggt tacctggatt
     2701 cttattaact atgaggaatg gtattttagc accaaatgca ggaattgata aatcaaatgt
     2761 tccttctaat aatgttatat gtcttcctat ggatccattt atctctgctg aaaatttgcg
     2821 aatggaattc ctcgtacaat tgggaattag ggttggaata gtcatttccg atagtagact
     2881 aatgcctaca aggatcggta ctactggtgt agctataggt tgttcaggaa tagaaccagt
     2941 agaagatcaa aggggtaaaa aagatctttt tgggcacatc atgaaatata cacttaaagc
     3001 aacagcagat tctttagcta caatggggac gtttgtaatg ggagaaagta atgagtcagt
     3061 tccattagtg ttaatacggg attcaaatat ttcctttact gatagaagac tatgttggca
     3121 agacgttgct atcgattatc aaacagacat ttattttcga ggcatccaaa actttgattc
     3181 tattatataa tatcttattt tcatagcatt ttaatataac catgatctcc aaattttcaa
     3241 taaccatatg tctgaattcc ttacgagata tccaaaaaaa atgagctttt taaaaggatt
     3301 acaggaatca atcaagtctg aagatatcca gtttgatcat cttagtctct tagtaaaaat
     3361 aaagaaagat gaaattatta aaactcttat cttggaggga ttttccaaag tcaagtttga
     3421 aaataaaaaa ccaggacaaa taggtaatgg atttacaaaa aaattaaaaa aaccctggga
     3481 aatccatatt agactactat atatgcatca gggattaatt gcaattcaag gagaagtgga
     3541 aatctcaaga agatatattc agcatatcag atcggtccgc gctccagtaa tttatgaaat
     3601 agaatctatt ctaaagaaac acaggataga atataaaatc tggaatgata aagtacgtga
     3661 ttacataact aatattattg aaaatcacca aataattctt aattcaccaa agttaccagc
     3721 aatgccttgg ttattaatga taatctttgg ttcaatgatt ggttctttac atggactaaa
     3781 actctttgga atattttaaa gaaagaaatt cttttatgat cttaatttga tattatttat
     3841 aatccaacaa tgggaattcc agagaaaatc aaagaattag aagatgagat acacaaaaca
     3901 caaataaaca aggctactga attccacatt ggaattttaa aagcaaagat agccaaactt
     3961 aaacgtgaac ttgacgaaaa taggcatgga aagacagtct caagcggaaa aggtagttcg
     4021 gggtatgacg ttagaaaaga aggagatgcc acggttgttc tcattggatt tccgagtgtt
     4081 ggaaaatcta cattattaaa ttctttaact aatgcaaatt caaagatagc atcatatcag
     4141 tttactacac taaccgctat tcctggcatg atggagtaca gaggagcaaa aatacaagtg
     4201 attgacttac ctggaattat taaaggtgca gcgtctggga aaggaatggg taaaaaagtt
     4261 ctctctgttg ctagaaatgc tgatttagta ttgattgttg ttgatgtatt ccaacccaaa
     4321 catttttcta tattagtaaa agaattagcc gaaataggta taaaggttaa taaaaaacct
     4381 ccgaatgttc acatacataa aactgacaaa ggaggaattt cagtcagtat cgatcaagat
     4441 tcacaagttt cagaatctat ggttagagag atgtgtagaa tctatggaat tcataatggt
     4501 agaattacaa taagagatcc taacttgact accgatgata taatcgatgc tttagctgca
     4561 aatagagtat atcctcattc cctaataatt ttaaataaaa tcgatctgat agattctaat
     4621 acattaaatg ctatgattcc ctttctaggc gaagccgtcg aacctgtttc agcaaattcg
     4681 aacctgaata ttgaaatgct taaggaatcg atttttaaca aattagaatt cattagaatt
     4741 tacatgagac ctaaaggaaa ggaaaccgat tatgaagagc ctttaattgt aaggaatgga
     4801 acaagagtaa aggacatttg tgccaaatta catagaaaca tgctccgtga cttccgattt
     4861 gcattggttt caggaaaaag tgcaaaattt gaaggacaga aagtaggact ggatcatgta
     4921 ttgttagatc aagatgtatt aacaataatc aaaaagataa atgctatatg aatacctttt
     4981 aaagtggatt gtttccctca attttacctt tttcatcatt cttcttttta atattcgtat
     5041 aaattagtat tcctattatt cctgctatta ttatataatt tacaggtgga ttaaggtatt
     5101 tcaaaactaa tccaatcttt ggaacggtat aggctactgt tccaatgtaa tctttttcgc
     5161 gaataggaaa atctgtccct ggtattatac catcgtttgc atcacccttt gttactatta
     5221 ctttctcccc aaatctatca ctaatctcta aaattctgtg aacaataact ctgtctcctc
     5281 cttgaggccg attaaaaaca ataatatcgc ccacctttaa gctattaaag gtatttccat
     5341 cttttactat caatatatca ccgacgttca gaacaggaat catgcttcca ctagaaacta
     5401 cataaaatgg attgtctaca ttgaatacga ctttgacagc aaaccatatc aaaactactc
     5461 cgataactat tattgcgata tcttttatga ggctaaactt cttgtcctta ttttcgagca
     5521 attctaatta aatttcaatt tatttgttac ataaactttt agattaaatg ttatatatgg
     5581 cagctcagtc aaatgccttt attacatatg attttcaata ttaactctga ttcttcatcg
     5641 ctgcctaaat aataagccta agcctgatta attaatatga tagtctatgg ccttttttgc
     5701 aactaaatct acagttgtat ttctttctcg tggtacatat ttgtaagaaa tttttttaaa
     5761 ttttttttct aaattattaa tctctctcga aagaattttc agttctttcc ttctaattct
     5821 ttccttcgat tttctgtgtt gtacgattgt catactatcc gtgtgaattc taatttcctt
     5881 atcaattata actgcaaatt gtaatgctct ttttagggct agatattccg ataaaagatt
     5941 tgtattaaat cctataaatt caccataact taatattaac gtattatttc gaaatacaat
     6001 aattcctata cctgaaggtc caggatttcc tctcgaggct ccatcaatat ggacagtaag
     6061 caatatagac aaatacttta tgaactaatt tattattttt catattctat tttgaatata
     6121 ttatgaagat tcattcaaaa ctagattaga aatctcgatt attgatatgg agtaattttt
     6181 tgtttttatc aatatcaata atttttataa agtgaaataa acctataacc cttattttga
     6241 ggagattaca ttcatagttt cccctgtaga gagtttaaat tctagaccaa atatctattg
     6301 acgcaacaaa aaagatatgg atcagactgt tgagatgtaa taaatattca gattgttaca
     6361 atgattaact atttatcaga atatatataa ggattggaga atgtagaaag actctaatat
     6421 tgagtttcta cgatttaatt tagaatgccc aaacggcggt tcaagccaac aaaacctaca
     6481 aaaaaaatag ggaaaaaaca attactatta ccacctgtta tgataggcat agcaactttc
     6541 gcaggaataa taataatgca attgtcacct cctccacaag ttttgggcat ttgcttaaaa
     6601 gctcataatg ttgaaacctt caatatttat ccagtagtgc aaatttttga agatgataag
     6661 caaaaatacc ttcctgaggg tatgggaaag gaaataaaag atggaaaaga atgcattcat
     6721 ctcattcaca cagacgaaat aggagataga atacatattg agtatattag acctattagg
     6781 cttacaatag atgattttat gcaactttac tcagttgata ataaaacaat aactgttctt
     6841 aataacgaaa caggatcatt taagaaagaa gtactcccgt tagaaaatta caatatccag
     6901 tattcttatt tctctgaaca taatgaattt acacagattt ctaattctac attaatgcca
     6961 cctttcacta aggatatggt agtaaaaatt gaattaaaat cgaaaatgtg gaagtaagaa
     7021 tggagttacg atccatataa aatataaata gaaacgtcca aatgaaaatt tgattatttt
     7081 tgggaaataa aaaggctgtt gttataaagg gagatggtac cggcccagag ttggtaaaat
     7141 gtatgactga tgttttgaaa tcatgtaaca gtactataga gcttatacct gtagaagctg
     7201 gatctgaatt ttggaaaagg aatcaaacca attcttatat cactagtgaa gtttggaaat
     7261 tactatcgga gagtgatgct tgctttaaag ggcctactac tacgatgcca gatccaaatg
     7321 ctcctaggag tgtagccgtg agcattagac agaaatttga actttatgca aatattagac
     7381 caataaaaac atataaaact ttacagttag atttggattt cgtttgtgta agggaggcaa
     7441 ctgaaggttt gtatgctgga atagaattta aaaccagtga cgattccgct gttgctatta
     7501 gaaaaataac cagaaaagga tgtgaacgtg tggttaaaaa agcatttgaa ttatgtaagg
     7561 aattagattt taaaaaaata tttgttatta ctaaaagaaa tattttaaaa gaaaccgatg
     7621 gaatatttgt ttca
//
LOCUS       DASVEV010000111         8467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_38286, whole genome shotgun sequence.
ACCESSION   DASVEV010000111 DASVEV010000000
VERSION     DASVEV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38286"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VF222_05085"
     CDS             <1..880
                     /locus_tag="VF222_05085"
                     /inference="COORDINATES: protein motif:HMM:NF044723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141833.1"
                     /translation="YLVMANAIRKLVDEEMESTIRGMYYATLSTVGDEKNKEKFWRSQ
                     EDSDDAIKAVELITGIPREEFSVTSKPKGMISGPITLNVGGDIIDCNLGSRATSQLIP
                     TNISDVEIVDVKADLVMVVEKDTVLNNIRKSGFIQKYNAILLTGSGEPDRATRMMVKT
                     LNEKWKKPVVIFADADPWGLGIALRYKIGSESLSYDSDRLVAPNVKVLGMMFSDIYDY
                     NIPEVARLSVTAEDINRANDMKKKPWLQEKQWQKELSLFLKKKEKCELDAFFKHGFKY
                     LAEQYLPKKLREAGVI"
     gene            980..1165
                     /locus_tag="VF222_05090"
     CDS             980..1165
                     /locus_tag="VF222_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-domain-containing protein"
                     /protein_id="HEX7141834.1"
                     /translation="MLEAKCPKCEKKAEVNEQMTEVNCSYCSTKIPYEEYLDIMKEKA
                     LNMGIEFHNNWDKNPYS"
     gene            complement(1197..2456)
                     /locus_tag="VF222_05095"
     CDS             complement(1197..2456)
                     /locus_tag="VF222_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HEX7141835.1"
                     /translation="MHWKDTLPDWYVREYGYQPNVNIGTAGHVDHGKTTLIEAITGIW
                     TSAHSEELRRGITIKVGYADAAFYKCKSCPSPECYSTNPQCNNCGQESELSRVASFVD
                     SPGHESLMANMLSGAALMDGAILVVAANEAVPRPQTREHMLALEVLGIKQIVIVQNKI
                     DLTEKEEALKNYDQIKDFVDNSVAKNAPIIPVSAQHNLNIDILIEAIEKNIHTPHRNR
                     QTFPIMHVLRSFDINKPGMPVGSIHGGAIGGALIQGEFNVADEIEMRPGNINNKGKYS
                     PIYSKISSLGTGAGLVSNVKPGGLVSIGTILDPSLVKSDSLIGSVVGKPGTLPEDIYD
                     VSIDTYLFDTAVGTQEPVKVEPIKKNEQLRINIGTLATTALVTNTKEHKIEINLKKPV
                     CIIESSRVALSRKIGERWRLIGSGVTK"
     gene            complement(2523..2924)
                     /locus_tag="VF222_05100"
     CDS             complement(2523..2924)
                     /locus_tag="VF222_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HEX7141836.1"
                     /translation="MVNFKIVISDKLGKSLSHELKDKDAQPFLGSKIGDTFDSSLVGL
                     AGGKLQITGGSDKSGTPMRSDLHGGVKKYVLLHTGVGMRDTTAGKRVRKLLRGNMITE
                     EIYQLNCILDGVNIVSSQPENIETSNTETTK"
     gene            3001..3138
                     /locus_tag="VF222_05105"
     CDS             3001..3138
                     /locus_tag="VF222_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141837.1"
                     /translation="MNSEQEKEIPICPICERKMIAIQACHLRCNNCGAELTCSDKGHF
                     W"
     gene            3793..4185
                     /locus_tag="VF222_05110"
     CDS             3793..4185
                     /locus_tag="VF222_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141838.1"
                     /translation="MAARKNTKPKKETPKETIVIKPTKEIKETKSVPITVELNILKVL
                     INERPLIPLLRVLKKNKELHTRELLITMGSWGYGQSLMKRATNLGLIERKVKKEKSRG
                     RGKPRIYNALTNKGREVVKLADRIGYEK"
     gene            4274..4651
                     /locus_tag="VF222_05115"
     CDS             4274..4651
                     /locus_tag="VF222_05115"
                     /inference="COORDINATES: protein motif:HMM:NF037434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141839.1"
                     /translation="MNILCDTSFLMVLVTKPIKLLDSIVIRYGKINFLVPDVVIEELK
                     ILVNKASYKRSQIAKTVLEVTKRFEIVNTNKSNYSDDSIIDYAINYKCAVVTMDKNLI
                     KRLISSKVMVFSLRNNKLIIMNP"
     gene            4694..5353
                     /locus_tag="VF222_05120"
     CDS             4694..5353
                     /locus_tag="VF222_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7141840.1"
                     /translation="MEINNIKLYWLGHDGFKLITPNKKIIYIDPYKISEVHHKKNDAE
                     IVLISHNHFDHLSIEDLRHIINENTKIIAASECIEKLNQEGFKNVKNVEPGQTIEEKD
                     ISIKIVPAYNINKNFHPKDDRKVGFIITFDKMVRIYHSGDTDKIPEMSEYKPDVALVP
                     VSGVYVMTAEEAADAVNNLLKPSKLTIPMHYGTIVGSLDDAIKFKKLVKICGVEILPQ
                     E"
     gene            5747..6295
                     /locus_tag="VF222_05125"
     CDS             5747..6295
                     /locus_tag="VF222_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7141841.1"
                     /translation="MVKTESSQKLKTGQKAPDFNLIGVDGKLYSLNDFNSKTLLIIFI
                     CNHCPFVKASIKDIIDLQSNFNKDELQIVGINSNDPNYEEEGFDNMKKFSKEYTINFP
                     YLIDETQEIAKTYGAVCTPDPYLFDQEKRLVFHGKLNDSQSPERTPTREIMKENIQKV
                     LKDEKIVKDFDPSIGCSIKWKG"
     gene            6341..8323
                     /gene="uvrB"
                     /locus_tag="VF222_05130"
     CDS             6341..8323
                     /gene="uvrB"
                     /locus_tag="VF222_05130"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX7141842.1"
                     /translation="MDKKDLFEFPLKSAYSPDGDQPYAIDQLQEGIFLGSQKSVQTLL
                     GVTGSGKTFTIANLISRIKKNTLVISHNKTLAAQLYSEFKDFFPSTNVGYFVSFYDYY
                     QPESYIPQTDTYIEKDTAVNETIEKMRLEATAMLMSGEPTIIVSTVSCIYSLGSPSEW
                     ERNALTITEGDMMNRKEIIKRLIESRYERNDFSLTPGNFRVKGEIIDIIPGYSNDIVR
                     ITLFDQEIEKISMHDATTLKKIRDLNRITIFPAKHYITDKKVRENAISMINKELKEWL
                     PKLEGELEKERLASRTKYDLEMINELGYCSGIENYSRHFDGRKEGEPPYCLLDFFGDD
                     FLLVIDESHVTLPQLRGMYNGDHTRKEMLVAYGFRLPSSFDNRPLKFDEFERYLRKVI
                     FVSATPGPYELKSSFKVVEQLVRPTGLVDPIVEIRSSKNQIEELIFEIQKRVTKNQRT
                     LVTTLTKRMAEDLAEFLSKKNIKVRYLHSEIEGLERTELIRQLRLGEFDVLIGINLLR
                     EGLDIPEVSLVVILDSDKEGFLRNDISLIQTFGRAARNIDGTVIMYADKITKSMENAI
                     AETNRRRKKQIEFNKLHHITPISISKPIVKDKNSQTQREYFKSMSKNDLRKLSVETEA
                     LMNKYAEELEFEKAIQLTEKLKKINQEIGIIEVSRS"
     gene            8320..>8467
                     /locus_tag="VF222_05135"
     CDS             8320..>8467
                     /locus_tag="VF222_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7141843.1"
                     /translation="MIDKLIVRGAKQHNLNNINIDIPKNKIVIFTGLSGSGKSTLAFD
                     TIYAE"
BASE COUNT         3175 a         1203 c         1376 g         2713 t
ORIGIN      
        1 atatttagtt atggctaatg caattcgaaa gctagttgat gaagaaatgg agagtactat
       61 tagaggtatg tattatgcaa cattaagtac ggtaggagat gaaaaaaata aagaaaaatt
      121 ttggagaagt caggaagatt ctgatgatgc tattaaagca gtagaattaa ttactggtat
      181 tcctcgagaa gaattttcag taacttctaa accaaaaggt atgatatctg gtcctattac
      241 tctaaacgtt ggtggagata ttatcgattg caatcttgga agtagggcaa cgtctcagtt
      301 aattccgaca aatatttcgg atgtagaaat cgtagacgtt aaagcagatt tggttatggt
      361 agtagaaaaa gatacggttt taaataatat aagaaaatcc gggttcatcc aaaaatataa
      421 tgcaatactt ttgactggtt caggagagcc agacagagca accagaatga tggtaaagac
      481 attaaatgaa aaatggaaaa aacctgttgt gatatttgca gatgccgatc cttggggttt
      541 aggtatagct ttacgctata aaattgggag tgagtcttta agttatgata gtgataggtt
      601 ggtagcaccg aatgtaaaag tgctaggaat gatgttttca gatatatacg attacaatat
      661 ccccgaagtg gctaggttat cggttactgc tgaagatatc aatagagcaa atgatatgaa
      721 aaagaaacca tggttgcaag aaaaacaatg gcaaaaagaa ttaagtttat ttctgaaaaa
      781 aaaggaaaaa tgtgagttag atgcattttt taaacatgga tttaaatact tagctgaaca
      841 atacctccca aagaaactta gagaagcagg agtcatctaa taaataactt ttatgaaatc
      901 tgttatattt ctatctaatt agacgtgatc tttcagaata atcttaaaca tttttaagaa
      961 gtaaatcact tttaagaata tgctagaagc taagtgtcct aaatgtgaaa aaaaggctga
     1021 ggttaacgaa caaatgacag aagttaattg ttcttattgt tcaacaaaaa ttccatatga
     1081 agagtatcta gacataatga aggaaaaggc acttaatatg ggtatagaat tccataataa
     1141 ttgggataaa aatccctatt cttagtaggt attggaaaga aaccttttaa ataaaattat
     1201 ttcgttactc cagaacctat tagacgccat ctttctccaa tctttctact taaagcaact
     1261 ctgctactct ctataataca aactggtttt tttaggttaa tttctatttt atgttctttg
     1321 gtatttgtaa ctaaagcagt agtagctaaa gttccaatat ttattctaag ctgttcattt
     1381 tttttaattg gttcaacttt cactggttcc tgagttccaa ctgcggtatc gaaaagatat
     1441 gtatctattg aaacatcata gatatcttca ggcaatgttc ccggtttacc tactacagac
     1501 ccaataagtg aatcactctt tactaaagac ggatctaaaa ttgtaccaat cgacactaat
     1561 cctcctggct tgacgttact aactaatccc gcaccggtac ctaaactgga aatttttgaa
     1621 taaataggag aatatttacc tttgttatta atattacctg gtctcatttc aatttcatca
     1681 gctacattaa attcaccttg aattaaagct ccaccaatag cacctccatg aatcgaccca
     1741 acaggcattc ctggcttgtt tatatcaaat gaacgtaaaa cgtgcattat tggaaatgtt
     1801 tgcctattcc tatgaggagt atggatattt ttctctatgg cttcaattaa tatatcaata
     1861 tttaaattgt gttgagcaga aactggtata ataggagcat tttttgctac actattatca
     1921 acaaaatctt ttatttgatc ataatttttt aatgcttctt ctttttctgt aagatctatt
     1981 ttgttttgga caattacaat ctgtttaatt ccgagtactt ctagagccaa catatgttct
     2041 cgagtttgtg gacgaggtac agcttcattg gcagctacta caagaattgc accgtccata
     2101 agagcagctc ctgacaacat attggccatt aaactttcat gacctggcga atcaacaaaa
     2161 ctggctactc tcgaaagttc actttcttgt ccacagttat tacattgtgg gttagtagag
     2221 taacattcag gggatggaca ggatttacat ttataaaaag cagcgtcagc gtatcctact
     2281 tttatagtta tacccctcct tagttcttcg ctatgggcac tagtccaaat tccagttatg
     2341 gcttcaatta aagtggtttt tccatgatca acatgtcctg ctgtacctat atttacatta
     2401 ggctggtaac cgtattcacg aacataccaa tcaggaagag tatctttcca atgcaatagc
     2461 gaataaagat ttattgaggg atattaaggt aattgataaa tattttataa agaaactttt
     2521 gtttactttg tggtttcagt attgctagtt tcaatatttt ctggttgaga tgaaacaatg
     2581 ttaacaccat ctaaaataca gtttagttga taaatttcct ctgttatcat atttcctctc
     2641 aataattttc taactctttt tcctgcagtg gtatcacgca tacccacccc agtatgtaat
     2701 aaaacatatt tttttactcc accgtgaaga tcagacctca taggagtgcc agatttatcg
     2761 ctaccaccag ttatttgtaa tttgcctcct gctaatccta caagagaaga atcaaatgta
     2821 tcacctattt ttgaccctaa aaatggttga gcatctttat ctttaagctc atgtgataaa
     2881 cttttaccca atttatccga aataactatt ttaaaattaa ccaatgaacg atctaaaagt
     2941 aatattatta attaaccttt tgcaaaaaag ttaaaatttg atctagcatt atattcaaaa
     3001 atgaatagtg aacaagaaaa agaaattcca atttgtccca tatgtgaaag aaaaatgatt
     3061 gctattcagg cttgccattt aagatgtaat aattgtggag cagagctaac ttgttctgat
     3121 aaaggtcatt tttggtgagg aatttatctt acaatatata aattctatta taatttaatc
     3181 aaatataagt tttgcaatca ctttgcacta gaatgttcat acttagttta tttataaata
     3241 ttcaaatttt cttccttttt ctgtacatat tttttctttg aaacgatttt catcaaacaa
     3301 tataattaat tgtaaattga gcacttctcc attactttaa attataataa atgtcctaat
     3361 tgtcattgat attatattat aaatcaaaga ttcgctatgt caaaccagag aattactttc
     3421 atcatataaa cttctattta atctgacaat aatattgatt tactatatat ttattcatca
     3481 ctatgaccat tgacttagcc tctaagtttt tgtaaaaaat ggagattcta caatgaattc
     3541 cttttgcact aattcatggt tgatgtatta acatttctga agaatcgtta acaggactat
     3601 tacaagatat tatcgaataa cattctacta taataatgga tacagattgt gaacaaaata
     3661 tttaagttat atatagattt agagtattaa aacatataac ttatactatt atttggtttt
     3721 aaatgatata aaaatcaata ggaaacaaaa tgattaaata tttgtatttt gtaaggtcac
     3781 atcggtagta aaatggcagc aagaaaaaac acaaaaccaa aaaaagaaac tcccaaggaa
     3841 acaatagtaa tcaaacctac taaggaaata aaagaaacaa aatcagttcc tataacggta
     3901 gaactaaata tacttaaagt tctcataaat gaacgtcctc ttattccatt attgagagta
     3961 ctcaaaaaga acaaagaatt gcatacaaga gaattactta ttacaatggg aagttgggga
     4021 tatggacaat ccttgatgaa aagagccaca aatttaggtt tgattgaaag aaaggttaaa
     4081 aaagaaaaat ccaggggacg agggaaacct aggatatata acgctctgac caataagggg
     4141 cgagaagtgg taaaattagc agatagaata ggctacgaaa aataaaatcc atccaaataa
     4201 attttctttt ttttatattt taaacatttc tcactcctaa caattataat aattaagtca
     4261 ttgaggaata ttgatgaata tactttgtga cactagtttc ttaatggttc tagtcacaaa
     4321 accgataaag ctgttggata gcattgtaat acgttatgga aaaattaatt ttcttgttcc
     4381 cgatgtagta atagaagaac taaagattct tgtcaataaa gcatcctata aaagatctca
     4441 aattgcaaaa accgttcttg aagtaacaaa aagatttgaa attgtcaata caaataaatc
     4501 aaactatagt gatgatagta tcatagatta tgcaattaat tataaatgtg ccgttgtgac
     4561 tatggataaa aatcttataa aaagactgat atcgtcaaag gtaatggtat tttcattacg
     4621 aaataataaa ttaatcatca tgaatcccta atttaataaa caactttaaa ttcttataca
     4681 aactacaatt tgtatggaaa taaataatat aaaattatat tggttgggac atgatgggtt
     4741 taaattaatc actcccaata agaaaataat atatattgat ccttataaaa tttctgaagt
     4801 acatcataaa aaaaatgatg cagaaatagt attgatttct cataatcatt ttgatcatct
     4861 tagtattgaa gatttaagac atataataaa tgagaatacg aagataattg cagcttccga
     4921 atgtattgaa aaattaaacc aagaaggatt taaaaacgta aaaaatgtag aacctggaca
     4981 gacaattgaa gaaaaggata ttagtattaa aattgttcct gcatataata tcaataaaaa
     5041 ttttcatcca aaagacgata gaaaggtggg atttattatt accttcgata aaatggtaag
     5101 aatatatcat tcaggtgata ctgataaaat acctgaaatg agcgagtata aacctgatgt
     5161 tgcccttgtt ccagtttcag gagtatatgt aatgacagca gaagaagcag cagatgctgt
     5221 taataattta ctaaaacctt ctaaattgac tatccctatg cattacggaa caatcgtagg
     5281 cagcttagac gacgcaataa aatttaaaaa gctagttaaa atatgtgggg tagagatttt
     5341 acctcaagaa tagaacaatt gtttttgcaa atctaaatat ttctttataa tattcgataa
     5401 cttaaacaaa ctataatata gatttactag ttatttatca ttctctgtat tcacttcaca
     5461 acgagtataa ttagattagc ttctatggaa tgttttttga taagtattat aatttggttt
     5521 gtggttatta atgattctta ataatataca aatgagccac ttatcaaatg ttatatttgg
     5581 aattaaatat taggaatcta ccttcaaaaa tttgtccaat ctggaatgaa gggttagatt
     5641 cattttatgc caaaaagata taactttagg tttgaaattt ttgtatattt tataatacat
     5701 tagtaaaacc cgaagattta tgtgagagta taaaaatgga atgaacatgg taaaaactga
     5761 atcttcccag aaattgaaaa caggccaaaa agctccagat ttcaatttaa ttggtgttga
     5821 cggaaaactt tattctttaa atgattttaa tagtaaaact ttattaataa tatttatctg
     5881 taatcattgt ccatttgtaa aggcaagtat taaggatatt atagacttac aatctaattt
     5941 caataaggat gaacttcaaa ttgtaggaat taatagtaac gatcccaact atgaagaaga
     6001 aggatttgat aatatgaaga aattttcaaa agaatacacc attaattttc cgtatttgat
     6061 tgatgaaact caagagatag caaaaacata tggcgctgtc tgtacacccg atccgtatct
     6121 gtttgatcaa gaaaaaaggc ttgtttttca tggaaaatta aatgattctc aaagtcctga
     6181 aagaactcca acaagagaaa taatgaaaga aaatattcaa aaagtattaa aagatgaaaa
     6241 aattgtaaag gattttgatc cctctattgg ttgttcgata aagtggaagg gttaaatata
     6301 ggacatcctc ttttatttat ccttttaagg taatcgttat ttggataaaa aagatttgtt
     6361 tgaatttcca ttgaaatcag cctattctcc tgatggggat cagccatatg caatagatca
     6421 gttacaggag ggaatttttc ttggaagcca aaagtcggtt caaactttgc ttggagttac
     6481 tggtagtggc aaaactttta caattgcaaa tctcatttct agaattaaaa aaaatactct
     6541 tgtaatctct cataataaaa cgctagcagc ccagctttat tccgaattta aggatttttt
     6601 tccctctact aatgttggat attttgttag tttttatgat tattatcagc ctgaaagtta
     6661 tattccccaa acagatacct acattgaaaa ggacacagca gtaaatgaaa ccattgaaaa
     6721 aatgaggtta gaagcaacag caatgttaat gtctggtgag ccaacaatca ttgtttctac
     6781 ggtttcctgt atctactcat taggttcacc atcagaatgg gaaagaaatg cactaacaat
     6841 aacagaagga gatatgatga atcgaaagga aataattaag aggttaattg aatcccgata
     6901 cgaaagaaac gatttctcgc ttactccagg aaattttcga gttaaaggtg aaattattga
     6961 tataatccct ggttactcta atgatattgt tagaataaca ctttttgatc aagagatcga
     7021 aaaaataagt atgcatgatg cgaccacact aaaaaaaatt agagatctaa ataggattac
     7081 aatatttcca gctaaacact acataactga caaaaaagtg agagaaaacg caataagtat
     7141 gataaacaag gaactaaaag aatggcttcc taaattagaa ggagaactgg aaaaagaaag
     7201 actggcttca aggacaaaat acgatctgga aatgataaat gaactgggat attgttctgg
     7261 tatagaaaat tattctcgtc attttgatgg tagaaaagaa ggagaacccc catattgttt
     7321 attagacttt tttggagacg attttttact cgttatagat gaatcccatg taacacttcc
     7381 gcagttaaga ggaatgtata acggagacca tactagaaaa gaaatgctag ttgcgtatgg
     7441 atttagactt cctagttcat tcgataatcg cccattaaaa tttgatgagt ttgaaagata
     7501 tttaagaaaa gtaatttttg tatccgcaac tcctggtcct tatgaattaa agtctagctt
     7561 caaagttgta gaacaattag ttagaccaac aggattggtc gatcctatag tagagattag
     7621 atcatcaaaa aatcaaattg aagaattaat ttttgaaatt cagaaaagag ttacaaagaa
     7681 ccaaagaaca ttagtaacaa cattaactaa aagaatggct gaagatttag cagagttctt
     7741 atcaaaaaaa aatattaaag taagatattt acactcagaa attgaagggt tagaacgtac
     7801 tgagttaatt cgtcagctaa ggttgggtga attcgatgtt ctaattggta tcaatttatt
     7861 aagagaagga ttggatattc cagaagtttc tctagttgtt atattagatt cagataaaga
     7921 aggtttttta agaaatgata tcagtcttat acaaacattt ggaagagctg ctagaaatat
     7981 agatggaacc gtaataatgt atgcagataa aattacaaaa tcgatggaaa atgcgatagc
     8041 agaaacaaat agaagacgaa aaaaacaaat agaatttaat aagttacatc atataacacc
     8101 tatctcaata tcaaagccaa tagttaagga caaaaattct caaactcaac gtgaatattt
     8161 taagtccatg agtaaaaatg acttgagaaa gctatctgta gaaaccgaag cattgatgaa
     8221 taagtatgct gaagaacttg aatttgagaa agccatacag ctgacagaaa aattaaaaaa
     8281 aataaatcag gaaattggaa taatagaggt cagtagatca tgatagataa gttaattgta
     8341 agaggtgcaa aacaacataa tttaaacaac ataaatattg atatacccaa aaacaagatt
     8401 gtaatcttta ctggactttc aggatctggt aaatctacac tagcatttga tactatttat
     8461 gcagaag
//
LOCUS       DASVEV010000112         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_38457, whole genome shotgun sequence.
ACCESSION   DASVEV010000112 DASVEV010000000
VERSION     DASVEV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38457"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VF222_05140"
     CDS             <1..853
                     /locus_tag="VF222_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7141844.1"
                     /translation="IVLNDEVKKRTKALRESNRQLSVSNQEIALANEELKVHDKMQKE
                     FINVASHEIKTPIQAILTFSQLLQRYPERQNEFTPAISRNSLRLQRLSNDILDVAKIE
                     SRSLKLNKELFDLNQVISNIIDDNKTIIVKENHKVKLLFNPSKEILLVEADKERIAGV
                     ISNLLSNAIKFTKEGEIFVSTEKKDNNNNNNNDHSYALVTVKDTGPGIDPEILPKIFS
                     KFITKSFEGLGLGLYISKNVVEAHGGKIWSENINNNNKNGYLGAIFYFTLPIINSNNS
                     SNNNIDK"
     gene            885..1250
                     /locus_tag="VF222_05145"
     CDS             885..1250
                     /locus_tag="VF222_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141845.1"
                     /translation="MSENKKILLVDDEADIALAFSLGLESHGFSVDAFTSSIKALANF
                     KADYYTLALLDIKIPEMDGLELYTKIRDKDKKIKVCFISAYDVDYHAVKEYSRCIIKK
                     PITIDDLINKINKEMQEKK"
     gene            1706..2065
                     /locus_tag="VF222_05150"
     CDS             1706..2065
                     /locus_tag="VF222_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141846.1"
                     /translation="MSQAEWFTTYLQYENLKDLLSVILYTSQSFMTITPLLYYISYKN
                     KEILFIHTGIIGGIVSHYHVIQEKPNKKYVELNKMTGDFKFVDNFSSSNNQSLYIPIV
                     KLVKSTLRFPIDNLEED"
BASE COUNT          966 a          305 c          356 g          825 t
ORIGIN      
        1 tatagtactt aatgatgaag taaagaagag aacaaaggca ttaagagaat caaatagaca
       61 actttcagta tcaaatcaag aaattgcatt ggctaatgaa gaattaaagg ttcatgataa
      121 aatgcaaaag gaatttatca atgttgcaag tcatgagata aagacaccta ttcaggctat
      181 tctaacattt tcacagctat tgcaaagata ccctgaaaga caaaatgaat tcactccagc
      241 gataagtaga aattccctta ggctccaaag actttccaac gacatattag atgttgctaa
      301 aattgaaagc cgatcgctaa agttaaacaa ggaattgttc gatctaaatc aagtaatatc
      361 aaatattata gatgataata agactattat tgtaaaagaa aatcacaaag tcaagttact
      421 ttttaatcct tcgaaagaaa ttttattagt agaagccgat aaggaaagaa ttgctggagt
      481 aatctccaac cttctaagta acgctattaa atttactaaa gaaggtgaaa tatttgtttc
      541 tactgaaaag aaagataata ataataataa taataatgat cattcctatg cgctagtgac
      601 agttaaggat accggtcctg gaattgatcc agaaatatta cctaaaatat tttcaaagtt
      661 tattacaaaa tcttttgaag gattaggtct aggattatat atatccaaaa atgtggtgga
      721 agctcatggt ggtaagatat ggtccgagaa tatcaacaac aataataaga atggctatct
      781 tggtgctata ttttatttta ctttacccat aatcaacagt aataattcat caaataacaa
      841 tatagataaa taaagaagga tggattagta aacataataa aataatgagc gaaaataaaa
      901 aaattttgct cgtagatgat gaagctgata tagctttggc ttttagtctc ggattagaat
      961 ctcatggttt ttcagttgat gcatttacta gttccattaa agcattagct aatttcaagg
     1021 cagattatta caccttggca cttttagata taaagatacc cgaaatggat ggtcttgaat
     1081 tgtatacgaa aataagagat aaagataaaa aaataaaggt atgttttata tcggcatacg
     1141 atgtggatta tcatgcagtt aaagaatatt caaggtgtat aattaaaaaa ccaataacaa
     1201 tagatgatct gataaataaa attaataaag aaatgcaaga aaaaaagtag aattagtaag
     1261 agattctaat catttaaaat agtgattgtg agattattat tattattttt attattatca
     1321 tcatcaaaga aattttctat aacgctttta ttgttaaatg gtaatatgaa actctgttat
     1381 ggttaatttc agaattataa ttattgtatg aaatattatc acgtatatct tgtcaggtaa
     1441 ataagttttg taaatcagaa tgtaatcttg aacataagaa atagataatt gtttcattct
     1501 gttacctgag ctagaggggg agagaggatt gtttgaaaat aatacatcaa caataatatc
     1561 ttaatcctac ctaaattttt ttctcgatct aaattgcttt aaaaaacaaa caaattaaca
     1621 atattcttgg aatgtaatta ttcttttatg aaagtagata agtatcaaaa gtctaaaata
     1681 atctagtgac attacttatt atcatatgtc acaagctgaa tggtttacta cttatttaca
     1741 atacgaaaat cttaaagatc tgttaagtgt tatactttat acctcacaat cctttatgac
     1801 cataacccca ttactttatt atatatccta taaaaataaa gaaatactgt ttattcatac
     1861 aggaataatt ggaggcattg tttctcatta tcatgtcatt caagaaaaac cgaacaagaa
     1921 atatgttgaa ttaaataaaa tgacaggaga cttcaagttt gtagataatt ttagtagtag
     1981 taataatcaa tcactttata ttcctattgt aaaattggta aaatcaacac ttagattccc
     2041 aatagataac ttggaagaag attaaagtat aattacaatt aaaatcgcat agtactatat
     2101 tatttactta ccatatcttt actttatacc agatataatg gtattaatag taagatagaa
     2161 gtgcagttcc ctctttgaaa tcgttttatt agaaacctcg aataactttg atttagtttt
     2221 tcgttaattt tgttgctaat tgttacataa cttttacaag attagttatt acaggtattc
     2281 tgaaagtgtt agttaaaatt ataagattaa cctaaatagt cataaagata aaggttctca
     2341 ttttatcaat tctcttctat tgttagatac atattttcat tttggcaaga tacatactta
     2401 gccgatcttt agataaaata ttaatattga ataaagccct attttataaa aa
//
LOCUS       DASVEV010000113        11036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_38783, whole genome shotgun sequence.
ACCESSION   DASVEV010000113 DASVEV010000000
VERSION     DASVEV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38783"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VF222_05155"
     CDS             complement(<1..848)
                     /locus_tag="VF222_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141847.1"
                     /translation="MKLTIVSNIVKDKIILKNNQITYSLGGPPCYCGLIAKQFGANVR
                     LVTKFGEDFNKNELLFFKNSNFEVNNNSLSQSPTTKFILKVNGSNRELYLLDKCDPIL
                     ISDLEQINTDCWIISPVLDEVPYTVLEYLINNYNNDFLMLDPQGYTRAIDTNSKVILH
                     ESLDPLFFKNIDAIKLDPQELFCFSGGNDINSLRKLKVNNNIRYALYSEKQRIHMLNE
                     DKHYWLDINKVNTSDSTGAGDILASAFTCALIKERDPLWAFSFGVGAVMASLKTKKNG
                     IEKIPK"
     gene            1101..1487
                     /locus_tag="VF222_05160"
     CDS             1101..1487
                     /locus_tag="VF222_05160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7141848.1"
                     /translation="MTKNYNNYHKSLETGKYINIQTYKKNGQAVSTPVWFISKDNRIY
                     FRTSNKSGKFKRIKNNNNVKFALCNFRGKIKGEWHNGLAKIDPDINRLIFSKINEKYG
                     IFAHVMNLLYKIKKMDIIILCIESRD"
     gene            complement(2138..3721)
                     /locus_tag="VF222_05165"
     CDS             complement(2138..3721)
                     /locus_tag="VF222_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7141849.1"
                     /translation="MTNNDDTPSRNKMNKGDKNIFNNDRDLAFKEKKRIVITSMIASA
                     SLAILKLIIGFSTNSLGILSESFHSGLDVLAALMTFYAIRIVIKPPDSKFTYGYAKIE
                     SLSSLAEILLLFLVAGYIFYEGLERIFFKSIEPDITFFSFAIMIISIIVDYWRSKTLF
                     RVARKYGSQAIEADALHYKADMISSSIVIVGLCTVLFFKIPNADAYTALIIACMIIYT
                     SLGLGKRTLDVLLDKAPKGINHMILESISGLEGVNRAHDVRVRNVGTAVFIDLHIEVP
                     RTSTNDKAHKIATNVEDKIRKIIPNSDVLVHVDAIESESETLTDIIRLIASETDGIKN
                     VHSIYFSQLPSYSNKQMNKNYPETNSNEGDYNELINNKITKEFRIKNINSNRLLHLYL
                     DVQVDSGLDLRAAHNIIESFESQVRKEIPVIQHITTHIEFETEEIKTIGIEKEVNSSY
                     IEKIRKSCFKIKGVVDCKDIGIVDMDGDQHITLTITIHSFNPSNLSIYDAHQIATDVQ
                     QIIMKDMGASKVVVHTEPL"
     gene            4249..4797
                     /locus_tag="VF222_05170"
     CDS             4249..4797
                     /locus_tag="VF222_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141850.1"
                     /translation="MEFINAQNNSTIILENEASKKSVQEKPKVEVSIVGTPNNDKIRG
                     GDGNDEIDGGEGDDIIYGKEGDDEFDGGNGDDILYGDEGEDTLDGGNGDDKLVGGIDT
                     DELKGGSGADLFLCDEDDKVIDFNSVDNDRSDGPCELIDKGLSPNNNENANNNDDFTN
                     GDLDGGEEEEDDDDLSSLGPIF"
     gene            complement(5167..6480)
                     /locus_tag="VF222_05175"
     CDS             complement(5167..6480)
                     /locus_tag="VF222_05175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7141851.1"
                     /translation="MESSFEELGVSTPIVKALNEYGYINPFPIQQESIPIFLKGLDMI
                     GQAHTGTGKTAAYALPILTKIQTRIPIQALILVPTRELALQVTNEINKFSKYIGIRTI
                     SIYGGQSIAIQQEQLRKGVQIVVATPGRLIDHLKRGSIQLDNVKFVVLDEADRMLDMG
                     FIEDIKFILFYVNEDRQTCLFSATMPSEIINLAQEYMHDYLEIRLNKDEISLDTIEQS
                     YLIVKEHEKFKHLCTIIQNRNKKQQTIIFVSTKQRTEKVFHYLKDDGFKTTMIHGDLS
                     QKQRNSAIYRFRNGMEDILVATDIVARGIDVPAVGHIINYDVPEDPDTYFHRIGRTAR
                     AGGSGIAISLVSNERMDSFTRIMRKTKQNMHKLNDEMGIEIPTIQRSPYRTYYRDRSY
                     NNYNNSGNNSSGNTTRYGYGNNYRGSGYRRSPYGNYRNNNDRREE"
     gene            complement(7372..8583)
                     /locus_tag="VF222_05180"
     CDS             complement(7372..8583)
                     /locus_tag="VF222_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7141852.1"
                     /translation="MKDQIDRLNNNDNENHNEFKEEPLFTEKEDNPSHLMNELPSNEL
                     IEYEKALELLDKLLMVNPDDPKLYYEKGFFFEIAQKYHAAIKSFEQAIHLDKNYFEAW
                     YRKGLALNNLEKYHEALKSFDQALIIKPKNINALNEKGYSLNKIGLYEQAAESYEKVL
                     KTNPDDIRALNNKGVALANSNHAKDAIYYYDLALNIKPDDFNALTNKGVALASLGKYE
                     DALYYYDLALSIKPYDINALNNKGVALAGLGRFDDALYYYDSAINIDSAIPKTYYNKG
                     ITLANVSQYEEAIECYDQVLRIDPDFIDAYYNKAVALGSLNKYQDAILYYDEVIYRNS
                     EYITAYYGKGYSLDCLQRYEDAIKCYDQVIRINPDYVDAYYGKGKALEKLGMTSEATK
                     YLTIAKKLKTF"
     gene            9404..10285
                     /locus_tag="VF222_05185"
     CDS             9404..10285
                     /locus_tag="VF222_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7141853.1"
                     /translation="MKIAAILSDYDGTLCPTTSLSNALPADLYNIILEISKVIPVCIV
                     SSKDFHYLDSCVSFALIISSIMGIETFLLQKNKKDFNYNFVNYEEISDPIELKNKITS
                     YSLPRYRIKYKEKLIQNSHMLEDLSSIISKNFHDIKILKKYTHKEKLLAGVSFDYRHL
                     LKWESYKMSIEPRIIDKISEFLNINSLPLNTLHIQTYLTHPFLDIYSIDCNKGDVVTD
                     VRTLLNLGDNKNILYLGDSENDNSAFRKADLSITIKSDERLNTKLDSHYSLEFNELSY
                     FLIKLYKENFNFTSMSI"
     gene            complement(10467..>11036)
                     /locus_tag="VF222_05190"
     CDS             complement(10467..>11036)
                     /locus_tag="VF222_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7141854.1"
                     /translation="VDFFINFLKKFLDILNIPDVIIIGSSFGGLLALEYAIKYNNMVK
                     KLVLVSPAGMMRYVSPTLKHYISAALYPTFENVLRAYKEMVYDSTSATEESILDFINR
                     MNLQNSKYAFMSTLMGFNDHPNLNDRLKIDIPTLIIWGRYDKLIPVRYAKKFKIPNSR
                     IVIMDHCGHYPHQEKTEIFTKTVMKFLDN"
BASE COUNT         3872 a         1564 c         1486 g         4114 t
ORIGIN      
        1 tttttaggga ttttttctat tccattcttt tttgtcttta atgaagccat tactgctcca
       61 accccaaagc taaaagccca caagggatcg cgctccttta ttaaggcaca agtaaatgca
      121 gatgcaagaa tatctcctgc acctgtcgaa tctgaggtat ttactttatt tatatcaagc
      181 caataatgtt tatcctcatt taacatatgt attctttgtt tttctgaata aagagcatat
      241 ctaatattgt tatttacttt taatttcctg agagaattta tatcattccc accagaaaaa
      301 caaaagagct cctgaggatc caactttatt gcgtcaatat ttttaaaaaa taatggatct
      361 aaactttcat gcagaataac tttactattt gtgtctatag ctctagtata tccctgtgga
      421 tctaacatta aaaagtcgtt attataatta tttattaaat attctaggac ggtatacgga
      481 acctcatcca atactggact aataatccaa caatcagtat taatttgttc taaatcagaa
      541 atcaaaatag gatcgcattt atctaataaa tataattctc tattcgatcc attaactttc
      601 aaaataaatt ttgttgttgg agattgtgaa agagaattat tatttacttc aaaattggaa
      661 tttttaaaaa atagtagttc gtttttattg aaatcctctc caaatttagt aactagccta
      721 acgttagctc caaattgttt agcgataaga ccgcaataac aaggaggacc tcctaaactg
      781 taagtaattt ggttgttttt caggataatt ttatccttta ctatatttga aacaattgtt
      841 agtttcattt ttatctttat atttatttta ttaaatttta actattgtaa atatggttgt
      901 ttaacattag agatgtaaca gcagaataat tcataataaa catttgattt aatctatttg
      961 gaattgagca ttattaattt aagtatattt ttgatgatac cgtttgagaa ttattttgat
     1021 ttcatcatac gttcaatata attaacccct attatattag taaattataa taataaaagt
     1081 gataataata gacatagcca ttgaccaaaa attataataa ttatcataaa tcacttgaaa
     1141 ctggaaaata tatcaatatt caaacctata aaaaaaatgg acaggcagta tcaactccag
     1201 tttggttcat tagtaaagat aataggattt actttcgtac tagtaataaa tctggaaaat
     1261 ttaaacgaat caaaaataat aataatgtta aatttgcatt atgcaatttt cgtggtaaaa
     1321 taaaaggaga gtggcataat ggattagcaa aaatagaccc tgatattaat aggttaattt
     1381 tttcgaaaat taatgaaaaa tatggcattt ttgctcatgt aatgaacctc ttatacaaaa
     1441 tcaagaaaat ggatattatt attttatgta ttgaatcgcg agactgaata tacaatagag
     1501 gttacaaaaa cacgaattat atcaataatt aattgtaata tattccaatc aaaaaaattt
     1561 gcaaagaatt catctatcct aattactata tcggaattta tcaatagaat acaacttatt
     1621 aatagataga ataattttaa tggctctcaa agtgcatttc aatcgtcaaa tcaatttgaa
     1681 gaatctaaat atagattgtt tatacttcct actatgtttt atctatacta atcaatagta
     1741 ttaatctgaa tatttttaat attatcgatt atattttcta gatgatgaac caaaacataa
     1801 tctgttttaa gagagaatat gcattgtaaa agactaataa atataataga atgttaataa
     1861 tcatcttaca attcgaataa acctcatccg tgtagaattt gaatcctcaa tgagaatgca
     1921 aattattatt ttttgctaac cagtataaat gttaataatg taaatccttg agagggtgag
     1981 ctaaacgcag tcacgcagaa agaaaatata attaacagta aaatgtaatt attattaaat
     2041 gtgctgtttc cctattatat tctatatttt aattcttata gaacattttg tatactaatt
     2101 ccctccttat gcttttcatt aacggtagaa ccaaaatcta taatggctcg gtatgtacga
     2161 caactttaga agcgcccata tccttcatta ttatttgttg gacatcggta gcaatttgat
     2221 gagcatcata tattgataga ttgcttggat tgaaagaatg aattgttatt gttaaagtaa
     2281 tatgttgatc tccatccata tccactattc caatatcctt acaatcaact acacctttta
     2341 ttttaaaaca ggatttacga atcttttcta tgtatgaaga attaacttct ttttcaattc
     2401 caattgtttt aatctcttct gtttcaaatt caatgtgagt ggtaatatgt tgtataactg
     2461 gaatctcttt cctcacttgt gactcaaaac tttcgataat attatgagcg gccctcaggt
     2521 ctaatccact atctacttga acatctaaat atagatgtaa caatcgatta ctatttatat
     2581 ttttgatcct aaactcttta gtaattttat tgttaattaa ttcattataa tcaccttcat
     2641 ttgaatttgt ttcaggataa tttttattca tttgtttatt tgaatatgat ggtaattgtg
     2701 agaaatatat tgaatgtaca tttttaattc catcagtttc tgaagcaatt aatcgtatta
     2761 tatcagttaa ggtttctgat tcagattcta ttgcatctac atgaactagc acatctgaat
     2821 ttggaattat ttttctgatt ttgtcttcca cattagtagc aattttatgt gctttatcat
     2881 tagttgatgt tctgggtact tcgatatgaa gatctatgaa aactgcggtt cctacatttc
     2941 tcactctgac atcgtgtgct ctgttgactc cttctagtcc agaaattgat tccaatatca
     3001 tgtggttaat gcctttagga gccttatcta aaagaacatc tagagtacgt tttcctagcc
     3061 caagagaggt gtatattatc atacacgcaa ttattaatgc agtatacgca tctgcgtttg
     3121 ggattttaaa aaacaagact gtacaaagtc ctacaataac aatcgaagaa gagatcatat
     3181 ccgctttata atgcagtgca tcagcctcaa tggcctggct accatatttg cgagcgactc
     3241 taaacaaagt ttttgatctc caataatcaa ctataattga aataatcatt attgcaaaag
     3301 aaaaaaatgt tatatctggc tcaatacttt taaagaatat tctctccaac ccttcataga
     3361 aaatataccc tgctaccagg aacagaagca atatttctgc aagactggaa agactctcaa
     3421 ttttagcata accatatgta aattttgaat caggaggttt aattacaatt ctaattgcat
     3481 aaaaagtcat aagagccgca agtacatcca ggccagaatg gaatgattct gaaagaattc
     3541 caaggctatt agtagaaaat ccaataataa gtttgagtat agcgaggcta gctgatgcta
     3601 tcatagaagt tatgactatg cgtttttttt ctttaaatgc taaatctcta tcgttattaa
     3661 aaatattctt atcaccttta ttcattttat tgcgtgaagg agtgtcatca ttattagtca
     3721 cgactcatat gatccttatt caaattaaat ataaatagta tttaaaatct tcaataaact
     3781 atgaggtttc tattgaattt ctttaaaaaa caatttttta ttttttattt tatatagaaa
     3841 atcaagtgaa aaatcatata agaatgtgac aatatgctta accatatcgt tataagtaat
     3901 ttttagaaaa aaccataaaa agtctagaat tatcaacaaa ttaaaacata aattagacat
     3961 atagaaggat tatttattgt atcttttatt aaataacata ttttgaaaaa aaattagcag
     4021 taatagaata ttatacaata aatcttaata gaatactctt agtaaggttc atcataattt
     4081 attaatactt ataagataat attaatttct aataaccaat taaatacaat ttgaaataaa
     4141 tttttttgaa aaatattgaa atgtataaat tggtattaat actataaatt tatgagattg
     4201 gtccggtttg gtttcttttt tatactattc tttttgaacc ttgttacttt ggaatttatt
     4261 aatgcgcaaa ataactcaac cataattctg gaaaatgaag catctaaaaa aagtgtccag
     4321 gaaaagccaa aagtagaagt atcaattgtg gggacaccaa ataatgacaa aattcgtgga
     4381 ggggatggca acgatgagat tgatggcgga gaaggtgatg atatcattta cggtaaagag
     4441 ggtgacgatg aatttgatgg aggaaatggt gatgacattt tatatggtga cgaaggagag
     4501 gatactctcg atggtggcaa tggcgatgac aaattagttg gagggataga cactgatgaa
     4561 ctgaaaggag gaagtggagc agatttattc ctatgtgatg aagatgacaa agttatagat
     4621 tttaactctg tcgacaatga cagaagtgat gggccatgtg aattaattga taagggttta
     4681 tctccaaata ataatgaaaa tgctaataat aatgacgatt tcactaatgg tgatctagac
     4741 ggtggggaag aagaagaaga tgatgatgac ctttcatctt tgggacccat attctaaaga
     4801 agttataata tttgatgatg aagcaatttc gaaactaaaa caaatactcc taatgttatt
     4861 tatatataga ttaaaaaaag cgcatcttat tagacaataa ttcgtttatg attttataat
     4921 atagaatacc aatatattca tattaacgat gatgatttaa taacggttgc acttaattcc
     4981 taataggcta ctgttatgta tatatttttt taacaattct agtatatata attattaatt
     5041 aaccagaaca atagtatacc ttatatcatt tataaataaa acttttctgc aaaactagat
     5101 atataaatca attaaaaaaa agatatatgt attatataca ttggttttta gtttctatta
     5161 gatgaactat tcttctcttc tatcattgtt gttgcgataa ttaccataag gactacgtct
     5221 atatccacta cctctataat tatttccata gccatatctt gttgtgtttc cagagctgtt
     5281 gttaccacta ttattgtaat tattgtaaga tctgtcacga taatatgttc gatatggcga
     5341 tctttgtatg gtcggtattt ctattcccat ctcatcatta agtttatgca tattttgttt
     5401 ggtcttcctc attattcttg taaatgagtc cattctttca ttagatacca gtgatattgc
     5461 gattccgctc cctcctgctc gagctgttct accaattcta tgaaagtatg tatcaggatc
     5521 ttctggtaca tcataattaa tgatatgacc tacagcagga acatcaatac ctcttgctac
     5581 tatatcagtt gcaacaagaa tatcttccat tccatttcta aatctgtata tagctgagtt
     5641 tctttgtttt tgagaaagat ctccgtgaat cattgtagtt ttgaatccat catccttcaa
     5701 ataatggaat actttttctg ttctctgctt ggtggagaca aaaataatag tttgttgttt
     5761 tttgtttcta ttttgtataa tggtacacaa atgtttaaat ttttcatgct ctttgacaat
     5821 caaatatgat tgctcaatag tatcaaggct tatctcatct ttatttagtc ttatttctaa
     5881 atagtcatgc atatattctt gcgcaagatt tataatttca gatggcatag tcgctgaaaa
     5941 tagacaggtc tgtctatctt cattgacata aaaaagtata aacttgatat cctctataaa
     6001 acccatatct agcatcctat cagcctcatc taaaactaca aattttacat tatctaattg
     6061 aatcgatcct cttttaagat ggtcaatcag tctacccggt gttgccacaa caatttgaac
     6121 cccttttctt aattgttcct gctgtattgc gatactttgt cctccgtaga ttgatattgt
     6181 tcggattcct atatattttg aaaatttatt aatttcattt gtaacttgca atgctaattc
     6241 tcttgttggg actaaaatta atgcttgaat aggaatacga gtttgtattt tagtcaatat
     6301 cggaagagca taggctgctg ttttaccagt gcctgtatgt gcctgtccta tcatatctaa
     6361 accttttaga aaaattggta tagattcttg ctggatagga aatggattta tatatccata
     6421 ttcattgagt gctttgacaa ttggagtact aactcctaat tcttcaaatg atgattccat
     6481 tttatttttt cacttttaaa ttgcacaaaa gcacggaaaa aatgagaagt aatgagtatt
     6541 aacttaacaa atctgccgta tttctcttgt aaatttatat ctgttaagct gcaatataag
     6601 gtatactaat atctcaattg aattatacct aaataccatt ataattacat tataattata
     6661 gagtagaaaa aatttacaaa aataattcta aatattctgg tttaccatat taattttata
     6721 ttatattgat cttttttata taactataag aaataaacta atactaagaa aactgtgaga
     6781 atagtttctg cagaaaagat gattttattt tattaatgca tttagagtag aattatattt
     6841 acttctacaa ttattttcta tttaacattt caagaaatta attattttaa ggtttttatt
     6901 taaattgatt ggacaatttt taagaattag atatagtttg gtaattcaaa taattggaaa
     6961 tactcaataa tatcaaatcg tataacgtat ttgcaactta atacgttagt tagatttcta
     7021 ttatattagt atttaattta agaatttaaa tctcatccga atgatcatct ttaacaaaat
     7081 taggtattac atatcattaa taccaaaagt ttgaaaacat atattacatt taaatttctc
     7141 ttttccaggg ttattagaaa taatctctaa ttgacttttg gttttacatc tagggcattt
     7201 tccatagaca gtttttcggg ctatacgaat ttttttcgtt tagctatata tatatcctaa
     7261 gtaaaaacta aattaataaa ttcagcatat tttaaaataa tattcataca tttttttaca
     7321 tggatatatg taaaaacttt attctggata aaagaaataa atatatcgaa gtcaaaatgt
     7381 ctttagtttc tttgctatag tcaaatattt tgttgcttct gaagtcattc caagcttctc
     7441 aagagccttt ccttttccat aataggcatc cacataatca ggatttatcc ttatcacttg
     7501 atcgtaacat tttatcgcat cctcatatct ttgtaaacaa tccagtgaat aaccttttcc
     7561 ataataagca gttatgtatt cactatttct ataaattact tcgtcataat aaagaattgc
     7621 atcttgatat ttatttaaac taccaagtgc aactgcctta ttataatagg cgtcaataaa
     7681 gtccgggtct attcttagta cttgatcgta acattctatt gcttcctcat attgtgaaac
     7741 atttgctaat gtaattccct tattgtaata cgtcttggga atagcagagt ctatgttaat
     7801 agcagaatca taatagtaga gtgcatcatc aaacctgcct aaacctgcaa gtgcaacacc
     7861 tttattattt aacgcattaa tatcatatgg tttaatactc aatgccaaat catagtagta
     7921 tagagcatct tcatatttac ctagactagc aagtgcaaca cctttattgg ttaaagcatt
     7981 aaagtcgtcg ggtttaatat ttaaggccag atcatagtaa tatatcgcat ccttagcatg
     8041 attactatta gctaaggcaa cacctttgtt gtttaaggca cgtatatcat cagggttggt
     8101 tttcaaaact ttttcatatg attctgcagc ttgttcataa agaccaattt tgtttaaaga
     8161 atagcctttt tcattcaacg cattaatatt tttaggctta ataattaatg cttggtcaaa
     8221 ggattttagt gcttcatgat acttttctaa attgttaagt gcaagaccct ttctatacca
     8281 cgcttcaaaa taatttttat caagatgtat tgcttgttcg aatgatttta tagctgcatg
     8341 atatttctgc gctatttcga agaagaatcc tttctcataa tataatttag gatcatcagg
     8401 attcaccatc aacagtttgt ctaaaagttc aagagctttc tcgtattcta ttaattcgtt
     8461 agatggtaac tcgttcatta agtgtgatgg attgtcttct ttttctgtaa ataaaggttc
     8521 ttctttaaat tcgttatgat tctcattgtc attattattt aatctatcta tttgatcctt
     8581 caataataat tatattattt aaataatcgt atttaagcaa tgctatattt tgtcatgttt
     8641 attttatttt agatatttaa aatagccctt aaaattcaag tgtgaaaaaa taaatgtctt
     8701 aaaaatggtg tatacaatat ctttcaaaca aatttttgaa atcttgtttt aattttgata
     8761 tttttatgaa ttttatattt tttaatattg ttcttaatat taatggtttt taagtttata
     8821 ataaaattaa actatatgat gtataaagaa tctattttga gtctctattt ataataataa
     8881 gaaaaattcc ataattactc ttgatggatc catattaata ttatttatgt tgatttttta
     8941 tttttattct tttgacagcg ggttttaaaa aaaatgttgg ccaaagtaat atagaacttg
     9001 tatactttaa tatgatgaga aagccaaaat tactgtcata gcgttataaa gacattttca
     9061 tatagtcaaa gatatattta aaaagtgttt agaagtaaaa atgtatagta taaatttcta
     9121 aatattttaa gtccctatta aagaacatat ttgtgccatc aattaactca ggatattaga
     9181 aggtggttaa atcaaatgta taagtttttt atttatcact ggagcatcag ttgattaaac
     9241 ctttctctat tggaacgaga ataaattaca ctattatctt ttcttaatct cataataaaa
     9301 tcttttaaag tggttgtctt atttataact gtaaaaaaat aagataaaaa aatgaaaaat
     9361 aatctaataa atagtatata tttgataaag aacatataaa tatatgaaaa tcgctgcaat
     9421 cctttctgac tatgatggta ctctttgtcc tactacttct ctaagtaatg cactcccagc
     9481 agatctttat aatatcattc ttgagatttc gaaagtcatc cctgtgtgca tagtatcaag
     9541 caaagacttt cattacttag attcatgtgt tagctttgct ctaattattt cttctataat
     9601 gggtattgaa acatttcttc tacaaaaaaa caagaaagat tttaactata actttgtcaa
     9661 ttatgaagaa atctccgatc caatagaatt aaaaaataaa attacatctt atagtttacc
     9721 tcgatatcgt attaaatata aagaaaaatt aattcagaat tcacatatgt tagaagattt
     9781 atcctccatt atttctaaaa attttcatga tataaaaata ttaaaaaaat acactcataa
     9841 agaaaagtta cttgcaggag tttcttttga ctatcggcat ttattaaaat gggaaagtta
     9901 taaaatgagt attgaacctc gaataattga taaaatttct gagtttttaa atataaattc
     9961 acttccactc aataccttgc atatccaaac ctatttaact caccctttct tagacatata
    10021 ttctatcgat tgtaataaag gagatgttgt aacggatgta cgcacgttat taaatttagg
    10081 tgataataaa aatattttat atttaggtga ttcagaaaat gacaattctg cttttagaaa
    10141 agcagatctc tcaataacta taaaatcaga tgaaagatta aatacaaaat tggattccca
    10201 ttatagtctt gaatttaatg agttaagcta ttttcttata aaattatata aggaaaattt
    10261 taattttact tctatgtcaa tttaaaaagt caataatatt aatgataaaa aaattataat
    10321 ctctgaataa acaaataata aacaaaaaat tacatagtag gctcacttaa ggctttaaag
    10381 gtaataaaaa ataatatatt cccccggaca tagctttgtt tcagtttttg tcaaaacata
    10441 aaatattagg taataaaatt ccaatttcaa ttgtctaaaa atttcattac tgttttagta
    10501 aaaatctctg ttttttcttg atgaggatag tgtccgcaat gatccattat aacaattcta
    10561 ctattcggaa ttttaaattt ttttgcgtat ctaaccggaa ttaatttatc atatctcccc
    10621 caaataatta aggtaggtat gtcaattttt aatcgatcat tcaaatttgg atgatcatta
    10681 aatcccatca aggtagacat aaatgcatat tttgaattct gtaagttcat tctattgata
    10741 aaatcgagaa tagattcctc agtagcactt gttgaatcat acaccatttc tttatatgct
    10801 cttaaaacat tttcaaatgt tggatataac gccgcagaaa tataatgttt taaggtaggt
    10861 gaaacatatc tcatcatgcc agcaggagat actagaacta gtttttttac catattgtta
    10921 tattttatag catattctaa tgcaagtaat cctccaaatg aagatcctat tatgataaca
    10981 tcgggtatat ttagtatatc taggaatttt tttaagaaat taataaaaaa atctac
//
LOCUS       DASVEV010000114         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1204240, whole genome shotgun sequence.
ACCESSION   DASVEV010000114 DASVEV010000000
VERSION     DASVEV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204240"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..595
                     /locus_tag="VF222_05195"
     CDS             455..595
                     /locus_tag="VF222_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141855.1"
                     /translation="MKSRKKLTSLMKQKKDQKLKPEVDLSEFNHNTRMTLNDNNEYVL
                     RY"
     gene            complement(943..1365)
                     /locus_tag="VF222_05200"
     CDS             complement(943..1365)
                     /locus_tag="VF222_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141856.1"
                     /translation="MDFNVSVGDDDQDTRILAPWFLNCLNRLSGSNTAKFILKQQVFA
                     QTPMNGFKADPEKYKKYIDLKGSPDYNSVVEPIIRFLERNRSLRISPVNPAKIAITPI
                     GVDKCNEPTDLEWSYNHTFYPIEDDDDIAKNPGHRNYK"
     gene            complement(1760..1894)
                     /locus_tag="VF222_05205"
     CDS             complement(1760..1894)
                     /locus_tag="VF222_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141857.1"
                     /translation="MPVKLSTTRNKIDLIDNTENRFLVKQYHTFMINFNFALLFIVFD
                     "
     gene            complement(2368..2703)
                     /locus_tag="VF222_05210"
     CDS             complement(2368..2703)
                     /locus_tag="VF222_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141858.1"
                     /translation="MDKKPVISDTYIPPTDAERTELIMVRVTPSEKELFETLTSILAK
                     LDVDGKCTKVIKNNSLSDFVRMSLLVVSNFYLYNIFQDSRVISRLVSNKAKKEFIAFR
                     KKYMNKFSP"
     gene            complement(2747..2965)
                     /locus_tag="VF222_05215"
     CDS             complement(2747..2965)
                     /locus_tag="VF222_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141859.1"
                     /translation="MLSSGISATFTLFITIYTATIINVLFAFTAFTPSQFTRNSLILV
                     NQEEKNKGISKGFVIRLITFMERFLFKI"
     gene            complement(3015..>3269)
                     /locus_tag="VF222_05220"
     CDS             complement(3015..>3269)
                     /locus_tag="VF222_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141860.1"
                     /translation="CFSLNAIIPDTIYNTNPGIPHSCIVSIRITPHKISANPTTFVFF
                     IQKTIKRPASLIAIISTEISTASAKKNPKIGIPIRGLIKK"
BASE COUNT         1175 a          456 c          495 g         1143 t
ORIGIN      
        1 aattgctgtt ctaaaagtaa ctgcaagcat cattttatag tccatattga atgcatttcc
       61 tacaatttct gataatacta attctatagc cataccctag attatttggt aatagacaat
      121 atttatttaa tattagtaaa aaggcataac ccacctctgg ggagagatac aaggctatgg
      181 ctttgttgaa gatgaatcct ataatttgcg tttaagattg tagataaatc tatgttatat
      241 tatttaagaa attggagact acgatagagt tagttgttag ttaaaaccat agccctctta
      301 tgagtccaaa ccaaagaaag aagaaggcta tggtgacgtt agtcttgcct ttcggagtgt
      361 ttaatcaatc gatattatat tttaataatt tgagactatg gtattctaat tggtttatca
      421 acagcggcaa tattacgagt catagattct cgtagtgaaa tcaaggaaaa agttaacttc
      481 acttatgaaa caaaagaagg accagaaatt aaagccagaa gtagatttaa gtgaattcaa
      541 tcataatact cgtatgacac taaacgataa taatgaatat gtattaagat attgaattct
      601 aacagtttat gaagcagcag aatttacagg agaacaggca atctacggat ctaagagcat
      661 tgataatact atgcataatt ataccagatt atcatctgaa gaaaaataga aagaattaga
      721 gaagtcattg gctaaaagaa aatgtataaa atagagtata ctagaaattg atgaaaaatt
      781 catagaaaga tgtaaatcta taggcaagac tccaaaatat caatacctac tcttacaatt
      841 gatattacaa tttcaagaag atataataga agtgaaaaag aagtttagta attttgtttt
      901 atctataatg actcgaacta attagtaatc agttgactat atttatttat agtttctatg
      961 tcctgggttc ttagcaatat catcatcatc ttctatagga taaaatgtat gattataact
     1021 ccattctagg tctgtaggtt cgttgcattt atcaacacca attggtgtaa ttgcaatttt
     1081 tgctggattg acaggagaaa ttcttaagga tcgatttctt tctaaaaaac gtataatagg
     1141 ctcaacaacg ctattatagt ctggacttcc ctttagatct atatattttt tatatttctc
     1201 tggatctgct ttgaaaccat tcattggtgt ttgtgcaaaa acttgttgtt ttaatataaa
     1261 tttcgcagta ttacttccag ataatctatt caaacaatta agaaaccagg gtgctaaaat
     1321 tctggtatct tggtcgtcat ctcctacact aacattgaaa tccattcaac tattaatcct
     1381 gcatgtatta tataaaaaag aaggtcaatt taagcagcgg gagagaagaa tgaagtgaaa
     1441 aagaagtttg gtatttttgt tttaactttc aacaccatag gttaatcaaa aattttgttc
     1501 ttattctgtt ggctaacaag gtgttattat caataaataa tttgttgtat taataagagt
     1561 atcaagatag gtaattaaaa aaataatgta tcaacaaaaa aataagaatg aaaataagaa
     1621 atacaattgt tattgtggag cagcatttgt tactttaaaa gaatattata aacataatca
     1681 ttactaccat ccagaatatc attaggagta gttagaaatt caaagttctc tactacctct
     1741 gcttctaaac tttcattttt taatcaaata caataaaaag aagagcaaaa ttaaagttta
     1801 tcataaatgt gtgatattgt ttgactagaa aacgattctc tgtgttatca attagatcta
     1861 tcttatttcg agtagttgaa agttttacag gcatctagaa agatctccta ctcttcttat
     1921 cgtaaaatgt atgtgtgtta acttatttaa tcccaactta ttagtgggcc taaaggaatc
     1981 acttacagat ctatactatt cctaaaattg atgaattact tgtaataata aaattattga
     2041 tatttgatag atagattcat tcccaatagc ttaaatatat tattgcattt gcacaaatca
     2101 aatttaaacg agaaaatgtg ttgattctca aattttctac accttctaac catgagccat
     2161 caaaagtctt tacattagga taaccaagaa gatatttcgg aacaaaccaa gtttatgatg
     2221 ctctttcacc aataccacaa taagtgcaat tctatagttg ggagtaatac cgaaagcagt
     2281 tctaaaagag gagttcaatg tagaaatact actaacatgt atttagctat tattgttaga
     2341 aacttattta aaatattttt tggaatatta aggtgagaat ttattcatgt attttttacg
     2401 aaatgcaata aactccttct ttgccttgtt actcacaagt ctacttatca cacgagagtc
     2461 ttggaaaatg ttatacaaat aaaaattaga tactactaat agactcatac gtacaaaatc
     2521 tgatagtgaa ttattcttta ttactttagt acatttccca tcaacatcaa gttttgccaa
     2581 tatagatgtt aacgtttcaa agagttcttt ctcagatggg gtcactctaa ccatgattag
     2641 ttcggtcctt tctgcatctg taggtggtat ataggtatcc gatataaccg gttttttatc
     2701 cattatttta ttttataaaa cgtccatata tcagatatgg tcactcttaa attttgaaca
     2761 aaaatctttc cataaatgta ataagccgta ttacaaatcc tttagaaatt cctttatttt
     2821 tttcttcttg attaaccagt attaaagaat tcctagtaaa ttgtgaaggc gtgaaagcag
     2881 tgaatgcaaa tagaacatta attatagtag cagtataaat agtaataaat aaagtaaaag
     2941 ttgcacttat accagaagat aacattaatt actacaattc ttagtcttat tgttccttta
     3001 ttggtgattt ttgtctattt ttttattaat cctctaattg gaattccaat cttagggttt
     3061 ttcttagctg atgcagtact tatttctgtc gaaataattg caattaagct ggcaggtctt
     3121 tttattgtct tttgtataaa aaatacaaaa gtagttggat tagcactaat tttatgcgga
     3181 gtaattctaa tactaacaat acagctatgg ggtattccag gatttgtatt gtatatagta
     3241 tctgggataa tggcattaag agaaaaaca
//
LOCUS       DASVEV010000115         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1401145, whole genome shotgun sequence.
ACCESSION   DASVEV010000115 DASVEV010000000
VERSION     DASVEV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401145"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2225..2605
                     /locus_tag="VF222_05225"
     CDS             2225..2605
                     /locus_tag="VF222_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141861.1"
                     /translation="MKTIYYLIIGLSIVILVVLFFIIKEQNKKPYSLEIDAIKDKTDI
                     SGTMYRVKVTNSGTNPLTGIKVTLGKGDIQNMDLLEPGQSFFFYPKPETNISIVKVTT
                     TEGIEKESDYRSPLKGIGLPGSGR"
     gene            complement(2993..3154)
                     /locus_tag="VF222_05230"
     CDS             complement(2993..3154)
                     /locus_tag="VF222_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7141862.1"
                     /translation="MSAIFEGTLVKWGTSVGLVIPKPIRDGMKLSAGDKIKLILQENR
                     LCIEKIEKG"
BASE COUNT         1320 a          518 c          481 g         1209 t
ORIGIN      
        1 gtaaaaaata ttctctgttt agagctattt ttgatgatat tactataaaa ttacaaagag
       61 acgttataac tatagaagct atcccaacag ttgatttagc tgaattaaca gcaaatgaat
      121 tttttgagat atgatataat aatttttttt atttatattt gtttgatcat aatgaacctg
      181 ctcattcatt ttccatttcg gtaaatataa tttataggtt tttcatgttt gtaaatgtga
      241 tagaaaacaa atatacgcca gcagcatgca atagtaattt taattataca gatgtatagt
      301 ttaaagttca ttttcggctg gaatcggacg aaactttgat tttgatgcat tcagaacaaa
      361 tataattact aactccggta acgatataac cgttcagagt gtagctgtgt gctttgacaa
      421 tccaccattg agaccttaac ttcctccttt ttatttttta taatattcga agattatctt
      481 ttaatatatt catcaattat aattattatt tttcaattga aaatatatct aaaatattta
      541 aatgttagaa caaaataatt taaaataagt ttctaataat gaaagctaat tacaatgtag
      601 tagaatccta ctacatggac gaatccactt ccgtctaact ttccttaaga catagataac
      661 cctactgtta aaaggtgaac ccgactaacg atatttacta tttaggttta taatgaaaag
      721 acatagtaag aatattcatt ctttaattag tacttgacct tttatactct acaagcaaat
      781 ccctatgtct tcttggaata atactggaat cgagttatag ttttgttaag atgaaaattt
      841 gtctatatat caatttattt agtgcaaagt tccaatcctc tgaacctgga aatcaagaat
      901 tttatttatc tgaacatatt agttattgtt taggattttt gatgaaattt ttagacgatg
      961 aaaaatcagt aaattaaatc aaaaagatca ttactaatac atcattagtt ccaaaaatgg
     1021 gattaattga ctacatcaag gtagataaaa aaatcattta aacatcatta gttccaaaaa
     1081 tgggattaat tgactacatc aaggtagata aaaaaaattt actaatgtaa tcattaaagt
     1141 tgctgaaaaa tccttaaata gacttttttc tatagaatag ttgaggatag tttgcaacct
     1201 ccccggtgat taaatcccac cgagttatca cactgggaca accaattatt gggctatcca
     1261 tcaccgttga acaacaacaa attacacaag aatgtaatta ctcctgctac tcgtaatagt
     1321 agcaggagta tcctactacc gttaaacaac aacaaattac acaagaatgt gtaatttttt
     1381 actagcagga ataattgcag tattacttta tatgctatct atttaaacct atatttataa
     1441 aacgttaaac aacaaccact tacagaataa taggaatatt tttagcgatt gtactagtcg
     1501 cacgaataat tcactttttt tctctatcag ttatggtcgg tagtatacaa gtcgaattag
     1561 aagatgaatt ggatagccaa caagaaatcc tatggaaatg ataattacga gctaaaagac
     1621 ctaacatatg gtaaagcaac agctattaca aatctacaga tagcactaat ataataataa
     1681 taaattgtat caatacaata tccatatctt taataacata attagtcttg ctgacttttc
     1741 tcaacaataa aacaaatgaa tgtagataga tacattctca tggatattta cttctataca
     1801 tatgatacaa attgtatggc aaatatttga tttaattata tgaattatct tatcgtaagt
     1861 caatttactg attctataaa taatattgga taagacgaat gttatgtgat gttagagaga
     1921 aatacgttgc caaaaagcta aatactatct agtagaaccc aactacatgg acgaatccac
     1981 taccgatggg ccagtagttt tctgggttca ctatatttga tactaataaa ccaagtagtc
     2041 ttttgacacg aggttctata ttcatccttt cttgtcatta tctataattc actaaatctg
     2101 acaaatgtag ttattttttg ctaattctct tctctattct cacatataat aaaatagcta
     2161 attaacctaa ctgtatatta caaagagttt ttatcaatac tttaaacata tacatacaac
     2221 ataattgaaa acaatttact atttaattat aggactttca atagtcatac tagtagtttt
     2281 attttttatt ataaaggaac aaaataaaaa accatactca ttagaaattg atgctattaa
     2341 agataaaacc gatatttcag gtactatgta cagggtcaaa gtaaccaatt caggaacaaa
     2401 ccctcttaca ggaataaaag tcacattggg aaaaggggat attcaaaata tggatttact
     2461 ggaaccaggc caatcatttt tcttttatcc taaacccgag actaatataa gtatagtaaa
     2521 agtcacgact accgaaggaa tagaaaaaga gagtgattat cgctctccgt tgaaaggaat
     2581 aggactacct ggaagtggaa gataataacc ataatatttt aagaatatag gaaaagtaaa
     2641 ataatactct ttgtttcaag aattattcaa aacctaaaag ctaactatat ctttgacaat
     2701 aatatatgac tatgacagaa tcaatagtag tctgtagaac cgagcgtgca aatcccgccc
     2761 actccatcgc tcttaagttg aaaggtttcg ataggatttg ttctaaaaat gaaggatgtc
     2821 aacaaattct ttatctaatt tattagcaaa atttaattta tggtaacatt tttgttaaca
     2881 tgaataatgt ttacttattg attaagttgt aataatgtta acatttcaga agtatggtaa
     2941 catagatatt ttccatgtta caatcaaagc aattgaaaag tagttaatga tattagcctt
     3001 tttctatttt ttctatgcat aatctatttt cttgcaaaat caacttaatt ttatcacctg
     3061 cacttaattt cattccatct ctaattggtt ttggaattac taatcctacc gatgtccccc
     3121 atttgaccag tgttccttcg aagattgccg acataggatt atattaaatt taaaaaatat
     3181 aaatgtagat atatatctaa atatagatta aaatatagat tatctgtata ttaagataaa
     3241 tattattatc attaggtaaa ataataataa taaattggga ttaggaaaaa ggaggaaatg
     3301 ataatcctag tattatttgt aaatgaagaa aaaagctatt acaataatac tactactatt
     3361 gccatttcta ttatctgcta ggttgtggaa tttttcccaa tcccaatcac tgaataacaa
     3421 attctggtag atggatgcta ttgccgtctc tatactaata gtggatgaac acacaggcat
     3481 tcaatggcat tagataccat gtgaaaatag acattattac tatagaga
//
LOCUS       DASVEV010000116        11759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_817862, whole genome shotgun sequence.
ACCESSION   DASVEV010000116 DASVEV010000000
VERSION     DASVEV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817862"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..482
                     /locus_tag="VF222_05235"
     CDS             270..482
                     /locus_tag="VF222_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141863.1"
                     /translation="MIGISLFLLWKKNWILSKDLKLNNEDENNKDDNHDINNQLKKKE
                     KIKEVYKLTNTKIRNFLILEIIHNCC"
     gene            594..1139
                     /locus_tag="VF222_05240"
     CDS             594..1139
                     /locus_tag="VF222_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141864.1"
                     /translation="MRVLNIAIDADGSPSGYISWHNHFNKQSKIRSIKPSLKNEKFGI
                     QRVEMLAIFFAISDNLKAFKKIKRTKQIVIIRSDSRSTIEQLNNKALINDEILQRIYG
                     SIKRMLQKICFVIVFDHLTRAKNRAGRILENIFRKNIKYIKKKRNAYKIERQCLHIKY
                     KESTSKYENTFTNLTKSVLIH"
     gene            complement(2179..3765)
                     /locus_tag="VF222_05245"
     CDS             complement(2179..3765)
                     /locus_tag="VF222_05245"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7141865.1"
                     /translation="MPFVFHYWDHNMGNHLLKYVILPSIFLSIIALGSVNSNLWVTSA
                     IHHFYIELFGTLLGAILAFYYISRAQTLNDKFSLFIGIGFLVNTLIDLFHVIVSFVYM
                     NDMIFLKYFIPQTWFAGRLFLSAMLSIAIIKYISFSSYFLDTKQQASSKLNKEEKLTK
                     FLLFYLVIFTLFASIVAISSLFVVFPFSVIDYLPLHRPYEIFPLALFLLAVYYFYKNK
                     IYKNNDVFYKSIIVSIVFDIFGQIIMSSSAHHFDTAHNIAHILKDASYFINIIGLALS
                     GVQYNNRLRETNEILSIQYQKVKESEKMKDEFINIAAHELRTPIQPILGLTEIMYSKV
                     KDEDQREILDIIIRNAKRLKRLTDNVLDVTKIESQSLVFNKEKLNLNTVISDVLKEYV
                     NKNITQEMAKIVYDFKLKNDIIVEADKDRLSQVIHNLLDNALKFTYYNQMIYVIVALH
                     KDKEQVIVSVKDTGEGISDEILPNLFSKFKTYSSSGTGLGLYICKNIIEAHGGKIWAE
                     NNSVGKGATFLFTLPIVDNL"
     gene            complement(4104..5276)
                     /locus_tag="VF222_05250"
     CDS             complement(4104..5276)
                     /locus_tag="VF222_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141866.1"
                     /translation="MKNFSSKGIRFSGDSIKSCVGFIDLVDSTKNTITMEGLDYIRRY
                     YSTFINSVSDLVKSSSGRVVKNIGDCLLFYFPKTSNDKNENSFREVIECALEILDKRY
                     SINDELSKQHLPPFNFRISIDYGVVDLALVGDYSQIDLFGSTLNLCSKINSSPSLSIP
                     NEIIIGDNFYRILKSFSAIVKDFNFSNNGDYKLTETTGYPTYNVKRKSYSSSSSSSYS
                     SPLSTTNINDTTNSYHDKQLSDSIFENSLDDSHYISSKEKGLFSHNKNNNNKRIILVD
                     DEQDILFTYRAFLKDYNYDVTSFTDPSAALNYIRDHKNFNDVLVVLDIRMKNLNGFQL
                     HQQIKSIDPTIKILFVTALDILDELLSIVPGVSKDQIMRKPVDRKIFTNTVKKLIN"
     gene            6510..6995
                     /locus_tag="VF222_05255"
     CDS             6510..6995
                     /locus_tag="VF222_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7141867.1"
                     /translation="MGRTAKEIVKANIEEVISDLNKAYADEWLAHYQYWLTAQWIRGM
                     DADTLKQVLLQQSADELMHAETLANRLIQLGGTPVMQFDQLIATAKCGYKEPPSDPAN
                     LKQVVQDVLEAEACAIESYNELSEKYRETDIVTKEIFEELLEDEVKDEEEWEKLGANI
                     E"
     gene            7397..8281
                     /locus_tag="VF222_05260"
     CDS             7397..8281
                     /locus_tag="VF222_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141868.1"
                     /translation="MTFPSRYEPKKKNLTDNTDVINNPTISNNNKDNEIDHVPTTIGD
                     AVKKVIETGKEVVIQGPQTAGELTGVKESEFEEEKTGKILKQGEKDQTNLDKVSKDSK
                     LEIDVIESATETSLLSNEEKIILNPNESETDEEAIITTVTTIPTEGVNVEAQTEFTVP
                     LKDKEENIEVETDVQVNPTASPSSGSSFESTNIKKPNLSLNDVSFEQKSTSLPESDKD
                     TINPKILLEKEDQTLQQQQQQTRKDFSSNPFITYSLLWQHIVSDWVSFYDEYLKNLIK
                     FNGFWFEKSDNSSSSSQQ"
     gene            complement(9297..9467)
                     /locus_tag="VF222_05265"
     CDS             complement(9297..9467)
                     /locus_tag="VF222_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141869.1"
                     /translation="MEKVFSTLRSFIDTTGKTKFCITCGGMATQEALFDVGDGVTLIE
                     KYCDTCAKKEVQ"
     gene            10634..11290
                     /gene="hsp20"
                     /locus_tag="VF222_05270"
     CDS             10634..11290
                     /gene="hsp20"
                     /locus_tag="VF222_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HEX7141870.1"
                     /translation="MSFPNDIDPYNFFRNLFGGSGGRRRREGRGFGGWNFDDMFREFD
                     DMRRETERIFSEQFKNIENKVPKDLIKEYETPEGGKVREVGPIVYGYSMTIGPDGKPK
                     IQEFGNLKSFSGRGSFEQPSLSAEREPLIDISTTDKEVKIVAEMPGISKEKIKIDAYD
                     KYVEIKSEDPNRKYHKKIEVPEDIDLESGKSNYNNGVLEIIFQKREQQKPKGRQINIE
                     "
     gene            complement(11382..>11759)
                     /locus_tag="VF222_05275"
     CDS             complement(11382..>11759)
                     /locus_tag="VF222_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7141871.1"
                     /translation="LTYDYLKDTSKSIKILMRIVGAIVVIAIIISYLSNDPVVAAAIG
                     TISGLVVGFASQNIIGNIIAGLYLALLRPFKLGDEITAFSNTGVIYDIGLISTKLKTT
                     DNKTVLVPNSSMVTTTIILNKRP"
BASE COUNT         4532 a         1546 c         1647 g         4034 t
ORIGIN      
        1 ctatttgaga ctaagtggaa ctgatgacat ttccgatatt aaattgtcat atttagaaat
       61 taatggccaa gtaagtttta tcaaaaaaaa gaaagtggaa caagtaacaa gttgaatcct
      121 agtaaataac cataatttta cataatacaa aggtagttta attaaccact ttcacttttg
      181 ataggtattt ttagcatgga aggatttgat ttaaataatc ttactttaat tccaaggcgt
      241 tttgctatat tggaaatggt tatggctaca tgataggaat ttctttattt cttctttgga
      301 aaaagaactg gatattatct aaagatttga aactcaataa tgaagatgaa aataataagg
      361 acgacaatca tgatataaat aatcaattga aaaagaagga aaaaataaaa gaggtatata
      421 aattaaccaa tactaaaatt aggaattttt taatacttga gataattcat aattgctgtt
      481 aaagagaatt ctacggtttg cttggatatt cttacacgaa aattatacat aaaaaaatta
      541 tttcaacgtt ttgcgtaatg actatataat atattttata gatataatgt agattgcgtg
      601 ttttaaatat agcaattgat gcagatggtt ccccctctgg ttatatttcg tggcataatc
      661 actttaacaa acaaagcaaa ataagatcta taaaaccttc cttaaaaaat gagaagtttg
      721 gtattcaaag agtggaaatg cttgctatat tttttgcaat ttctgataat cttaaagcat
      781 ttaagaaaat aaaaagaaca aaacaaatag tgattattag aagtgattcc cgttcaacta
      841 tagaacaatt aaataacaaa gccctaataa atgatgaaat acttcaaaga atttatggat
      901 ctatcaaaag aatgcttcaa aagatttgtt ttgtaatagt atttgatcat ttgacaaggg
      961 caaaaaatag agcaggaagg attttagaaa atatttttag aaaaaatata aagtatatca
     1021 agaagaaacg taatgcatat aaaatagaaa gacaatgtct acacattaaa tataaagaat
     1081 ctacttcaaa atacgaaaat acatttacta atttaacaaa atcagttttg attcattaaa
     1141 caaagtcaat aaattttttc tccagaaaaa atagttttta ttttagttac ttatttttct
     1201 cttattttac ttcataacta ctaaaggagt ctctttgctg gataacatac aaaagaataa
     1261 gtttacatat aatcgtatcc atttgtttta attctacttt aatttattat aaatcataag
     1321 acaaaaacga atcactgaca attaactata cacaattacg accattcttt tagtttggaa
     1381 caatagttta tacaatatca tcactatact ggtcatatat tatttgcttt tgatcctgga
     1441 attgataata cataataatg aagaaatagg aacaatagtt ttaaattata ggtttatatc
     1501 aattgcaaaa actattctat catttacgat gaatgaaatt aacctatttc tattttaatc
     1561 tagtggaaga gtgaatacaa atgttgcgca ttttccatct ggtaattatt ccctgttata
     1621 cttccttcat gtgatgctat aatatttttg aaatgtataa acctagtcca gtacctgctt
     1681 ctgattttgt ggaaaatttt tcgtcattgt ttctacttta ttttataacg aggtagccag
     1741 ttcatctgat tatagctctg tggtatcttc gatacttggt aatgcgatat tatttatttt
     1801 gttggaatta gctatgaaga agtagtaaga agatattgaa ataaagaata aaaaaatgat
     1861 taatgttatt actataagta tacgagatga ttgaaagtat ttaaaaaatg ataatgacat
     1921 aatgtggaga aaattttcta ggtattaaaa gttaatattt atttatactt aaatcaaatt
     1981 aaggttttaa ttatgacttt ctcaaataac ttgattgtac ctggaaccct agatacagaa
     2041 cattagaaaa cgaaattgta gaaattttag atagagtcaa tacgaaatat tcaatatcaa
     2101 gatctagttc cgatgtttcg cttacttaat agattgtcga atatttatta tcattacaaa
     2161 ggtattgttg ttctgccttt atagattatc gacaattggt aatgtaaaca gaaaagttgc
     2221 tcctttacca acagaattgt tttctgccca tattttccca ccatgtgctt ctataatatt
     2281 cttacagata tatagtccca atccagtacc ggaactagaa taagttttaa attttgaaaa
     2341 caaattaggc aatatctcat ctgagatacc ttctccagta tccttcacac tgactataac
     2401 ttgttctttg tctttgtgta aggctacaat aacatatatc atttggttgt aataagtaaa
     2461 ttttaaggca ttatctaaaa gattatgaat aacttgagat agtctatctt tatctgcttc
     2521 tacaataata tcatttttta gtttaaaatc atatactatt tttgccattt cttgtgtgat
     2581 atttttgttt acatattctt ttagaacatc agatatcact gtatttagat tcaatttttc
     2641 tttattaaaa actaaagatt gactttctat cttcgttaca tccaatacat tatcagtaag
     2701 ccgttttaat cgttttgcat ttctaattat tatgtctaat atttctcttt gatcttcatc
     2761 tttaacttta gaatacataa tctctgtcaa tccaagtatc ggttgtatag gggttctcaa
     2821 ttcatgtgca gctatattaa taaattcatc cttcattttt tctgattcct tcactttttg
     2881 atattgaata ctaagtattt cattggtttc tcttaatcta ttattatatt gtacacctga
     2941 taatgccaat cctataatat tgataaaata actagcatcc ttcaaaatat gtgctatatt
     3001 atgggcagta tcaaagtggt gtgcagaaga tgacattatt atttgtccaa aaatatcaaa
     3061 tacaatagaa acaattatac ttttataaaa gacatcatta tttttataga ttttattttt
     3121 atagaaataa tagactgcta ataaaaataa tgctaacgga aaaatttcgt atggtcgatg
     3181 taaaggaaga tagtctatca cagaaaatgg aaaaacaacg aagagagagc taatggctac
     3241 aatagaagca aataaagtaa atataaccaa ataaaataaa aggaattttg ttaatttttc
     3301 ttccttattt aattttgaag aggcttgttg ttttgtatct aaaaaataag atgaaaaaga
     3361 aatgtacttt attattgcta tagacaacat agcacttaaa aacaagcgcc ctgcgaacca
     3421 tgtttggggg atgaaatact tgagaaatat catatcattc atatatacaa atgaaactat
     3481 aacatgaaac aaatcaatta aagtattcac gagaaaacct ataccaataa atagactaaa
     3541 tttatcattt aacgtttgag cacgagatat ataatagaat gcaagtattg ctccaagcaa
     3601 tgtaccaaat aattcgatat aaaaatgatg tatagctgat gttacccata aattagaatt
     3661 gactgaacct agcgcaatta tcgataggaa aattgaaggc agtatgacgt atttaagtaa
     3721 atggtttccc atgttatgat cccaataatg aaagacaaat ggcaaattga acttcaccct
     3781 ctcttttttt ctttttttaa gcacgaaaaa aatacaagca acttgagaaa accatagata
     3841 tttttttgta tcaatttgtt atggttatac atctagtaca gattagaaag catcgtagag
     3901 gatataagaa tatctattca tttggacaaa acatagaata aacaatatct taaaattagt
     3961 ctatatttta tcatcaataa gataaataac atgagaaaat aaatgttttt aaactctaat
     4021 aaaaaacgtg tttatagaaa aagaaaattt agatgacaat ttaggtttta ccgttctatc
     4081 atgattttta atgtaatagt tatttaattt attaattttt ttacagtatt tgtaaatatc
     4141 tttctatcaa caggttttct cataatttgg tcttttgata ctccaggaac tatacttaat
     4201 aattcatcta atatatctaa agctgttaca aacagaatct taattgttgg atctatagat
     4261 tttatttgtt gatgtaattg aaatccattc aggtttttca ttcgaatatc aagtactact
     4321 agtacatcat tgaagttttt atgatctcta atataattga gtgctgcaga aggatctgta
     4381 aaagaagtta catcataatt gtaatctttt agaaaggctc tataagtgaa aagtatatcc
     4441 tgttcatcat cgactaaaat tattctttta ttgttgttat ttttgttatg cgaaaataaa
     4501 cctttttctt tactactaat ataatgacta tcatctaaag aattttcaaa tatggaatca
     4561 cttaattgtt tatcatgata agaatttgtt gtatcattaa tatttgttgt actaaggggt
     4621 gaggagtatg atgatgagga tgatgatgaa taactttttc ttttaacatt ataggtagga
     4681 tatccagtag tttctgtaag cttataatca ccattatttg aaaaattaaa atccttaaca
     4741 atagcagaaa aagatttcaa aattctgtag aaattatctc caataatgat ttcattcgga
     4801 atagacaatg aaggagatga atttatcttt gaacaaagat tcaatgttga tccaaataaa
     4861 tctatttgac tataatcacc aactaaagcc aaatctacta caccataatc tatactaatc
     4921 ctgaagttga atggtggtaa atgttgttta gacaactcat cattaatact ataacgcttg
     4981 tctaatattt ccaatgcaca ttcaataacc tctctaaaag aattttcgtt tttatcatta
     5041 gaagttttag ggaagtaaaa tagcaaacag tcaccaatat ttttaacaac tcttccacta
     5101 ctacttttaa ctaaatcaga gactgaattt ataaatgtag aatagtatct tctgatataa
     5161 tctaaaccct ccattgtaat ggtattttta gtcgaatcta ccaagtcaat aaagcctaca
     5221 cagcttttta tggagtcgcc agaaaatcgg atgcctttag aagaaaagtt tttcattaag
     5281 tcatacctct actaatagta attatgtgaa ataaatagta caaatgtaat agaagcataa
     5341 aagctgttat atttatattc atgacctgac tatcgttatc attttataaa aatatatttg
     5401 acaaagtaac gatttcgcta tagaacccct gggctctcta ctcatagtga atgaagcaag
     5461 tgtaacgact ttttataaga ttgtatgtta ttttatctca tcaactaaaa aaactgagca
     5521 aaatatttgg tttgtaaata aatgtgttag aaataatatg agtagtaatt tagattacaa
     5581 ttacaattaa tgtaatgaaa ataaaatttt tcggcgccga aaaattatca tcttaatata
     5641 ttgaaggatt gatataaata acaataacaa gaatgtaata gcgatctctt cttaactacc
     5701 gacttcttta tgaactagct tataccatat taattgtaaa agatatctat tttgttttat
     5761 tataattaat cttgtgataa tgtaactaat tctattttta gatctgttct attttcaatt
     5821 aaatatttaa tacttttttt ttgatgattt gtttttctat ataatattta ttattttaat
     5881 gacgtaaatc tcattatcta ttaaagtaga cacagattat tgttaattca tttttagtaa
     5941 aataaaatta tataattatt tcaataaaaa aaaccctaaa gaaatttgaa atttatttat
     6001 taaattcaat ccttagataa tttattgaag aatcatgaat caaataacta cctgtcttta
     6061 cattcaagaa tatgttatca gtaatgataa catatttaaa ggatcttttt tagcaatagt
     6121 agattttata aaaaaaagga aagacaaata atattgtata catctcatga taaataatta
     6181 atatattttt gaatattggt tttagtagat ttttataatg ataatggaac aactagtaca
     6241 tttttataaa gtactttgta catatattat atgacataga atttatttta ttcttttttt
     6301 aagaaattaa taaatgaatc gcttttctaa ataaatcgga atagatcaga tactaaagtg
     6361 aagaggaaaa tataacattc tagttatgtt atataataaa aatttagtta ctttttcccc
     6421 ggtctctttg ttgaaattaa atgtaaagaa tgatatttgt aaggacatat gataaatcct
     6481 taactaatat gttagtagtt attatataaa tgggtagaac agctaaagaa atagttaaag
     6541 caaatattga agaagtaata tcagatctta acaaggctta tgcagatgag tggcttgctc
     6601 attatcaata ttggttgaca gctcagtgga ttagaggtat ggatgcagat acattaaaac
     6661 aggtactatt gcaacaatca gcagatgagt taatgcacgc tgaaacattg gcaaatagat
     6721 taatacaatt aggtgggaca cctgtgatgc aatttgacca acttattgct acagcaaaat
     6781 gtggttataa agaaccacca tcggatcctg ctaatttaaa gcaagttgtt caagatgtgt
     6841 tggaagcaga agcatgtgct atagagtctt acaacgagct aagtgaaaaa tatcgagaga
     6901 cagatatagt cactaaggaa atttttgaag agttgctgga agatgaagtg aaagatgaag
     6961 aagaatggga aaagttagga gctaatatcg aataagtaat aaagacatat tcttttctcc
     7021 tttttattaa tctgagaaaa attataacct acaggcgaaa tcccattaat cagaaattga
     7081 attggaacaa agaaaaaccc gcatttttaa taactcgcct tacaattcat tgattgttct
     7141 atctttgcta atataataca acctgtttct ttgaaatcta taataattca aatcttgtta
     7201 tttaaataaa gttaattaaa tccaccatca atacttaata tgggatatat actaaatctt
     7261 ttagccccta ttcatctctg atgatttgaa taaatcaatt ataaaatcta tataagttta
     7321 atctcgtatt tttgcaaata gctccctaca tattgtagaa aaaaggttta tgaaacagat
     7381 atgatatgat atagttatga cctttcctag tagatatgaa ccaaagaaaa aaaatctaac
     7441 cgacaatact gatgttataa acaatccaac tatctcaaac aataataagg ataatgaaat
     7501 tgaccatgta ccaacaacta ttggtgatgc agttaagaaa gttatagaga ctggtaaaga
     7561 agtagttatt caaggtcctc aaactgcggg agaacttaca ggcgtaaaag aaagcgagtt
     7621 tgaagaagaa aaaactggca aaatactcaa acaaggtgaa aaagatcaga ccaatttgga
     7681 taaagtaagc aaggattcca aattagagat tgatgtgatc gaatctgcaa ctgaaactag
     7741 cttattatct aatgaagaaa agataatttt aaatcccaat gagtcagaga ctgacgaaga
     7801 agctattata acaacagtaa ctacaatacc gactgaagga gtaaatgtag aagcacagac
     7861 agaattcact gtccctttaa aagacaagga agaaaatata gaggtagaaa ctgatgtcca
     7921 agtaaatcct accgccagtc ctagttcagg ttcctctttc gaatctacaa atattaaaaa
     7981 acccaatctt tctctcaatg atgtatcatt cgaacaaaag agcacatctc tcccagaatc
     8041 tgataaagac actataaatc ctaaaatact actagaaaaa gaagatcaaa cattacaaca
     8101 acaacaacaa caaactagaa aggatttttc atctaatccg tttattacat attcactatt
     8161 gtggcaacat attgtatcag attgggttag tttctatgac gaatatctaa aaaatcttat
     8221 aaaatttaat ggattttggt ttgaaaagtc tgataattca agctctagtt cacaacaata
     8281 acaataattt tttcaactcc cataagggaa taattaaaaa attttataat gttaatatta
     8341 taaacttcct aaataattta gttaacctat gtaatatcta tataaaattc aataaaaaac
     8401 tacgataaga acttgcaatc accatttgaa aaattttatt tatagttaat attaaattta
     8461 attggcttaa cttttgttaa tgtggtgaaa gcgttaaaaa ttatgaattt cttttaccat
     8521 aaaaataaat gcaaatagca ttgaagatat aaaaatgata ttacataaat tgttagcata
     8581 acgatatttg aaccttacaa ttaattttct actttattac tatttatatg ctagaatgtc
     8641 ctaacgatta gtaactaaaa tgtcaagtta aaaaatgaaa atagtaaaag gtaaaatagc
     8701 aataacaata taatatacga agctgaaaaa aaactttaaa caaacaaagg caacagatag
     8761 aaaatactgc gtttaatatt gtagatacaa caaataaggt taccgatact atcaatgaat
     8821 ctcaaaaaat aaataatcag aatatggaaa aaattcaata cataaccaaa tacccactac
     8881 aaacaattaa tacagtccaa tcaatactaa gtgatgatat acaatttcaa aagaatatac
     8941 ctttctttta gcttttttta gcttatgcaa tatcgattat taatgttata tacaaataaa
     9001 aataaaatac accaaccgca cagttatgga atatttcata gattagagaa tagatttcca
     9061 aattttattg caatgacaat cgcttaagag tagttactga ctaatctcca tgaggataga
     9121 ttttaaatat cagagtttga tattataata taataactag taggccagaa ttaaaaaaaa
     9181 gacggcatga tattaatttt tatagattag aattccaaga aagctgaaaa aaatctcaat
     9241 caggaagtgt atcagatgat caaatgatta tattggatgc accttgtaaa aaggtgttat
     9301 tgtacttctt ttttagcgca agtatcgcaa tatttttcga tgagggtgac accgtcacct
     9361 acgtcaaata atgcttcttg agtagccatc ccaccacaag ttatgcagaa tttggtcttt
     9421 ccagtagtat caataaatga tcttagcgtt gaaaatactt tttccatttt attggtttcc
     9481 tctccttgaa aatgaatctg atgttaataa cgatactgca gttataccta tcacatcaac
     9541 catgaatgta ctattgttat tcatattatt atgttgattt ttatggtata tgaacttgcc
     9601 taatataata tcagttatag atagctgtta tccggtatag ataatcttga ttataattta
     9661 ggttaaagtt tttttgtaac tgtaacttga atctatattg atgatggaga attggtatat
     9721 aacttccttt tctttttgat tttgtaattt atttcacaat cacaaatttg agattaaatt
     9781 attatatcat atgagccacg tccaaaaaat gtaatgtact attaagagat ttgaaaaaat
     9841 aaaggaattc aagaatcagc atatcaaaaa agcattagag ttagataata aaggttcgaa
     9901 ttctagttag gttaatacgc tcaaaagact aaatggatta ttgttaaaaa ataacatttc
     9961 tattatatta attatccact ttattagtaa aatttttcaa ttcagataag tatatagtag
    10021 tataaagatt tttataaatg ttaaagcaat cgatttcaga tattttattt ctcccgagtt
    10081 tgtcacatat actgagtttt tgattatgag atccattgaa gactgtatca aagaaatagt
    10141 tttaatatga ttgatctcgt tacgattatt attacagata gcatatttag taaagatcaa
    10201 taacatattg atattattta tactagtata aaatattaat gataaggcaa cactatcacg
    10261 actggtaatt ttacctaaac ttactggctg gactatttat taaactaaac tgtataagat
    10321 atatatccat aagatgtaat ttccttagtt taatcgctcc tcccaccata aaatagaata
    10381 tttgaagtac taattgactt ccagcaaaat tgttaattag aaaagcattt tagagattta
    10441 actaaataga aaaaacatgg aatattaatt tgaacatcta ttaccacaaa atctaataga
    10501 cacgtaaact catcactagg acaaagaata gctttgtttt attttctgat tttactattt
    10561 gcttaaatac attagttgtt aatctagaat tcattttata taactttaat acaaggtaat
    10621 gtcttattat catatgagtt ttccaaatga catcgatcca tataatttct ttagaaattt
    10681 gtttggtggt agtggaggac gcagaagacg tgaaggaaga ggttttggtg gatggaactt
    10741 cgatgatatg ttcagagaat ttgacgacat gagaagagaa acagaaagaa tattttcaga
    10801 gcagtttaaa aatattgaaa ataaagtacc aaaagatcta attaaagaat acgaaacacc
    10861 agaaggcgga aaagttagag aggttggtcc tatagtatat ggatactcta tgacaattgg
    10921 tcctgatgga aaaccgaaaa tacaagaatt tggtaatcta aagtcgtttt cggggagagg
    10981 atcctttgaa caaccatcac tttcagcaga aagggaaccg ttaatagata tctcaactac
    11041 agataaagaa gtaaaaattg ttgcagagat gcctggaata agtaaagaaa aaataaaaat
    11101 agatgcatat gataaatatg tagaaattaa aagtgaagat cctaatagaa aatatcacaa
    11161 aaaaatagaa gtaccagaag atattgatct agaaagcggc aagtcaaatt ataacaatgg
    11221 agttcttgaa ataatattcc aaaagagaga acaacaaaaa ccaaaaggca gacaaataaa
    11281 tatagaataa ccgattaatt gaattgatac aatactaagc ttatttttat gattttcaca
    11341 ttattgcata aatgaaaaat aaaaacagta taatcacacc tctacggtct tttatttaat
    11401 ataatagtgg tagtaaccat agaagaatta ggaactagaa cagttttatt gtcggtagtt
    11461 tttagtttgg ttgagattaa gccaatatca tatattactc ctgtattact aaatgcagta
    11521 atctcatctc ctaatttaaa tggcctaagt aaagccagat ataaccctgc gatgatattt
    11581 cctattatat tttgagaagc aaaacctact accaaaccgg atatagttcc tattgctgca
    11641 gctacgacgg gatcattact taaataagaa attataattg ctattactac tattgcgcct
    11701 acaatcctca tcagaatttt tatagatttt gaagtatctt ttagataatc ataggtaag
//
LOCUS       DASVEV010000117        18871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1012096, whole genome shotgun sequence.
ACCESSION   DASVEV010000117 DASVEV010000000
VERSION     DASVEV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012096"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..1630
                     /locus_tag="VF222_05280"
     CDS             527..1630
                     /locus_tag="VF222_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7141872.1"
                     /translation="MSEDFVKVSDTKDIRPSHMKEVQLDGENICIVNVEGKYYAIGSI
                     CTHEGGPLADGTLEGYEVECPWHGSKFDVRTGEVINPPASEPEPVYQVKVDGNNILIK
                     KQGKSKSPQIELTLLEKDKFDSTDVMSFKFTKHNDDQIDNKTVNYIAGQFAFFDIGEV
                     YNDPKGPIRHFTISSSPTENFIMFSTRIRNSPYKKRLSTLEKGAKVKLRGPEGQFVLH
                     RDYSKYAVFLSGGIGVTPFRSMIKYATDTQLPVKITMFDSNRDRNNILFKKEFDDWAS
                     INKNLKIIYTISEEKEQHNEQSPLSIANDWDGEYGRIDKPMILKYIDTNVLNNSKFYI
                     CGPPSMLKAMQALLQEDLEIPKDRIKVEEFTGY"
     gene            complement(1823..2812)
                     /locus_tag="VF222_05285"
     CDS             complement(1823..2812)
                     /locus_tag="VF222_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141873.1"
                     /translation="MIKKNNDQSLLKIVVTIFIPVFFIFSLVISIQVFGEEDANNKQN
                     ATLALTDYNFIATGDWYCNEETKKTINNILDTHPELIITTGDQVKESPSAACWMKMSE
                     PIKNKMKIAIGNHDAEFANIYKQIVNYHQLKNPYYSHDFKNVHFISMSTEHPYEEDSK
                     QYKFIKSDLEKSTKNPNIDWIVVHQHKPLYSTKQDKQEAEQLRDVYEQLFQKYDVDLV
                     ISSHNQYYERTYPILYNENYEKTTNKKAEPQPIITDHSQSEYPPTDGIVFLTVGTAGD
                     ELDPIKEKHDFYVIQVSKFGFLNIEIENKGKTLVGAFHANDGGIIDQFKLNET"
     gene            complement(3967..5025)
                     /locus_tag="VF222_05290"
     CDS             complement(3967..5025)
                     /locus_tag="VF222_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141874.1"
                     /translation="MIVLFPTMALAEDKSKMNNNLTSSLSTTSINTTNVDFATNVEFI
                     KGHLSAAISNKQAGNNELTQTHTFHPITEIYTLIRDQLSSADNKLNKTLIDSLNNLTS
                     IVFTSQPAEFVDSATFVKNQFDKALVTVIPENEIANIKFNMSTLINLLKTAAAEYEIG
                     IDNGTITNIAEYQDAQGFVSSANELFKKTTSNLNQTMANEFTKIKDSLYKLNTSISNR
                     DDERQVSTIINNITNTIPNILNVRADDLAILASTDTESSELINNIRTLLDQIIEKAKN
                     GNYQNAESLVIEAYLDNFEFLEGEIAKHDESLMKDTEVLLREQLRNLIQGKAGIEDIQ
                     EMANKINSKLNEIEKIIQ"
     gene            complement(5691..6209)
                     /locus_tag="VF222_05295"
     CDS             complement(5691..6209)
                     /locus_tag="VF222_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141875.1"
                     /translation="MLLKNSQNSIVRETNIILKYEKRKMKYMVKNFQLMIMINSKNSL
                     YAISSGILMMMILLSPLHTIKAQEENEESDPLTILSNIRNLLDKSITELNTGNYTGAS
                     ELVDIAYIDNYEYIEDPLKELDNDLMEETEIMIREDFASAIEDKKSLDEITTLHNAIK
                     SNLDIAEKLFKK"
     gene            6481..7719
                     /locus_tag="VF222_05300"
     CDS             6481..7719
                     /locus_tag="VF222_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7141876.1"
                     /translation="MSTLFQWISIDGKLLLCARIVRTFAYGFLSVILAIYLKLIGFDD
                     LLIGIILSATLINSVIFTLFASFYADRLGRRNVLLLYAIMMSISGAIFFITENPLALI
                     IGALIGTLNITGSETSAFLSIEQSILPQTIKDNRKRNTLFGFYNMAGTFAMGAGILIV
                     NLPILIQNEFEFDQIYAIKLLFLFYSLLGILIIGIYLKLSSSIEIKKEKLFKPLSKTL
                     TPKSKKIVAKLSGLFAIDSFAGGFAIQSIVSFWFFTKFDIDLSTISYIFSIGSVLTAF
                     SYLIAAKIADKIGLINTMVFTHIPSNILLILLAVAPNLEIAILFYMVRMALSQMDVPT
                     RQSYIVAVVEEDERTASAGITNLSRNIAQAISPSITGYIIGFLSLSAPFIIGGLLKIL
                     YDITLYINFRKIKPAEEEQD"
     gene            8680..8904
                     /locus_tag="VF222_05305"
     CDS             8680..8904
                     /locus_tag="VF222_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141877.1"
                     /translation="MFNKLFLLSLFLILPLQTSFAQINQTSIDQVDNTTTVTTEDSIV
                     GDKLEQVQNQSNGVGKIIEELVTEEPLEKE"
     gene            9021..9203
                     /locus_tag="VF222_05310"
     CDS             9021..9203
                     /locus_tag="VF222_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141878.1"
                     /translation="MSNTINEETCYYATYAKINGYPTEKECSNPATRHIENNATSQQF
                     PVCEEHYQLVSKQSES"
     gene            complement(9219..9446)
                     /locus_tag="VF222_05315"
     CDS             complement(9219..9446)
                     /locus_tag="VF222_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141879.1"
                     /translation="MSNSLGLLQGSIKEIQSNTSFYLILYRSRRKGSWFPKRYSPKTG
                     LRPLSLLDAASDYEDDTKWFSYECNHYPMTA"
     gene            9597..10028
                     /locus_tag="VF222_05320"
     CDS             9597..10028
                     /locus_tag="VF222_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7141880.1"
                     /translation="MFRSNIVTNYITLTSEAENDIINITNDVERILIESHLKNGILTL
                     FIVGSTAAITTIEYEPGLKKDFPRLLERIAPKEIEYEHDKTWHDGNGHAHVRASLIGP
                     SLTIPFINGQISLGTWQQIVFVEMDTRGRERKIVVQLIGND"
     gene            complement(10275..10736)
                     /locus_tag="VF222_05325"
     CDS             complement(10275..10736)
                     /locus_tag="VF222_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141881.1"
                     /translation="MMIRDSLSNTVFSHSNSNDKNNAGLGKNKQWELSSRNIVKKPIK
                     FNELCPIWSKKLSLGLDRVDKFLIERDSKYCIVGEAWKFSGRYNGYYFALLIPLVGCW
                     TCIKYAHKFAKISKNKNKKEALIRSDFEPLIDYFMKHWNETHLNKKYLINK"
     gene            complement(11637..12614)
                     /locus_tag="VF222_05330"
     CDS             complement(11637..12614)
                     /locus_tag="VF222_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141882.1"
                     /translation="MNNKLSKSSPTMLIIILAGIAIVSASSYSIDEAMAFPHASLIID
                     PKEEEHANDIRVVLGHTNEPAYGKLPGIHDGKHNLEVDLSDDATTLPIPNATLFADKY
                     YFKNIASFQRAESLEDADAIELNVPISAVFGQPGTFYNRQVIDAGIYGYTIRGTINYY
                     DVAQVPLENATKFCSIPGEDLTKFDTDGWNGSYGCPQNIKSIFFPPASKYPDNNYNNY
                     PPPQYDQYDDGYGNYQQQQQQYDNNNNDYGYVKEDYKQYDNNNNNNNNYDDKRDNGRD
                     DKKNDKKDDSKEYDKNYDNNDNNYDYQYRYEYTDDNGEYGNEYASYGEE"
     gene            12789..13631
                     /locus_tag="VF222_05335"
     CDS             12789..13631
                     /locus_tag="VF222_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141883.1"
                     /translation="MLPQKSTILAVMMATLLSLGSLSLMPLDVYASHSYEAGVQGDAI
                     PDNTHIDLSQLTLPSAGVFPLYDSSPNFVSGHFLYRAPCDPGTKIPIVSVIAGHIDES
                     ANLTHVDFVPLYYVGHASPLPGICVYHAHLPDPLEGGSPRVTDVDLVNFSPRNVTFNP
                     GDAVDVNVQRTLGSIGNYYANDVKLPLELAGGNNKVLDLNAHVAATANNVNAQQTLSN
                     LVQLNADYMKSPFEFTGGNNQILNLNEENDNNNNDNNNDNNNDNNNDNEDENDNDNED
                     QEDE"
     gene            14524..14685
                     /locus_tag="VF222_05340"
     CDS             14524..14685
                     /locus_tag="VF222_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141884.1"
                     /translation="MEDSTVIASWTRMKTNSAIKNSLKPPKIHFNAFDTTFVAILLRT
                     LTETGSCSI"
     gene            complement(14849..15382)
                     /locus_tag="VF222_05345"
     CDS             complement(14849..15382)
                     /locus_tag="VF222_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7141885.1"
                     /translation="MSTINHKRKTNSSQDKIVKYVNEIPGIRYRELLRKTGLSNGVLS
                     YHLRSLDNSGKIRVNRVNNRVTRYFSYDVSSHESYVIGLLRQETTRKIILYMLENGAC
                     GFNDILTHARKVPSTISWHMGRLKAANIVKVRKQNEFNYYEIGMDRKILQDLLSKYTR
                     SFTEKIVEDYVDMINEF"
     gene            complement(15386..15802)
                     /locus_tag="VF222_05350"
     CDS             complement(15386..15802)
                     /locus_tag="VF222_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141886.1"
                     /translation="MLRNYNSPGTFYNKPRLLVLIASIVISLSVVPFILPHLFHQHMI
                     YHILMHLVALIISQFLAVVSIMAYLKSRTTRILFMTLGFVTLVIAEYVYLLNSTEDFH
                     AMFIPQVNIELSHFILLIMIIFFGISFLKSPQVKQV"
     gene            15979..16611
                     /locus_tag="VF222_05355"
     CDS             15979..16611
                     /locus_tag="VF222_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141887.1"
                     /translation="MQNIKKYSIIAILTLSVVAGIFSNIPLSYAQNDTMSMSNMTEED
                     ATTTEAGHSAEEYEKEYNETSTVRDSVTELLQDKVIPGGDFLHLYDSTPYHILNGHVA
                     LKAPCGDDSTTPIQILIGSAPNMTVTTLENIAPLSTPGEQCLYHADLIPSGNVTIITD
                     IALKNPTEEDMEFPPTATVVVGINEITKGEHGHSESEEEHAAATEAGQTE"
     gene            16715..16858
                     /locus_tag="VF222_05360"
     CDS             16715..16858
                     /locus_tag="VF222_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141888.1"
                     /translation="MLLVGLLFPLSINSVSSKDEEFVEIPAEEYYDPADYGLKPYSSP
                     GTG"
     gene            17478..18410
                     /locus_tag="VF222_05365"
     CDS             17478..18410
                     /locus_tag="VF222_05365"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7141889.1"
                     /translation="MSIFLVINSYNVQGAEEYQFKIKFGSKGVDNGKFIQPIGIAVDK
                     EDNIYVGDFARHSNIIQKFSDNGTFISSGGTLGNSPGFFTNPVSLEIDRNNGFLYISD
                     FGNPNFAIQKFALNGTFLKSFGEFGLGDGKFINPGGIGLDSQGNVYVTDFGENSNVQK
                     FDKNGNFLQSIGSHGIKQGQFNNPTDVAVDSIGNIFVTDSGNERIQKFDKNGNFVMTF
                     GTFGHNDRQFDNLLGIEIDENDNIFVTDSENHRIQKLNKDGVFLTSFGKFGSGDGEFI
                     HPSGIDIDSKGNVYVTDERNYRVQIFSPVTSYFN"
     gene            18602..>18871
                     /locus_tag="VF222_05370"
     CDS             18602..>18871
                     /locus_tag="VF222_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141890.1"
                     /translation="MSTKRHDRKEEESNETHSTTTTEQQFQREQQQAVNKALDETRDN
                     IKKTTNEARKEIPRYTQIVNDYQENTIQAIREITDNYVESQKDIIV"
BASE COUNT         6742 a         2660 c         2745 g         6724 t
ORIGIN      
        1 ttcttgaaat atgtcatcat atggttccac aaaccaccat tgctgctttt cttcattaac
       61 ttctatttga gaaattttca atactgtcat ttagttttca attgtttatc agtgtcattt
      121 tatataaaat tttaattttc tgtctgaaat ccttttttag tctatatttg attcatattc
      181 gattgagaca aatacaaaat atttttcaag aaattttctg aacggcaagg aacaagatag
      241 ttaataggat gaaataaaaa aactatacaa tattaaaaaa aatgatattc catatcataa
      301 tgggtcagtt ttaccttaac cgaaaccaca tatttgataa atacagtaaa gggaataatg
      361 aagatttaaa gactaaccag atactatgcc accatatgtg ctactttatc tactcattcc
      421 cgctaaagtg gaaaaataat tgatccaagt gttggatcat aattgtgtct gcaagtttag
      481 ctagaaaacc atatagcctt ttaacattat aatgaaaatg atcgtattga gtgaagattt
      541 tgttaaggtt tctgatacaa aggatattcg accttcacat atgaaagaag ttcaacttga
      601 tggagaaaat atctgtatag tcaatgttga gggaaaatac tatgctattg gcagtatttg
      661 tacgcatgaa ggtggtcctc ttgccgatgg tactcttgaa ggctatgaag ttgaatgtcc
      721 atggcatggg tccaaatttg atgtaagaac aggtgaggtt ataaaccctc ctgcaagtga
      781 accagaacca gtctatcaag taaaagtaga tggaaataac atattaatta agaaacaagg
      841 taaaagtaaa tctccccaga ttgagctaac actattggaa aaagataaat ttgatagtac
      901 tgatgtaatg tcttttaagt ttactaaaca taatgatgat caaatagata acaaaacagt
      961 aaattatatt gcaggtcaat ttgccttttt tgatataggt gaagtctata atgatccaaa
     1021 aggtccgata cgacatttta ctatttcatc ttctccaacg gagaatttta taatgttcag
     1081 cactagaatc agaaattcac catataaaaa gagactttca actttagaga agggtgcaaa
     1141 agtcaagttg agaggtccag aaggacaatt tgtattgcat cgggattatt caaagtatgc
     1201 tgtatttctt tctggaggca taggtgttac tccatttaga agtatgataa agtatgctac
     1261 agatacgcaa ttgccagtaa aaataacaat gtttgattcc aatagagatc gtaacaacat
     1321 cctattcaaa aaagagtttg atgattgggc aagcataaat aaaaatttga agattatcta
     1381 taccattagt gaagaaaaag aacaacacaa cgaacaatca ccattatcaa ttgcaaatga
     1441 ttgggatgga gagtatggaa gaatagacaa accaatgatt ttaaagtaca tagacaccaa
     1501 tgtactaaat aattcaaaat tctatatctg tggtccgcct agcatgctaa aagctatgca
     1561 agctttgctc caagaggatt tagaaattcc aaaagacaga atcaaagttg aagagtttac
     1621 aggttactga ttactgatga tgatgactac acaccggata atttcgaaat gaagaaatct
     1681 taattagaaa taaattttat gcctttggaa acaaataata tgtatttctt tcaaatatgt
     1741 atttctttca gagttggcat tattattaat aaagaaattt aggatacatc ttctacaatg
     1801 attagacaag aatcaaaaaa tattatgtct cgtttagttt aaactggtca attatccctc
     1861 catcattggc atgaaatgca ccaactaatg tcttgccttt attttctatt tcaatattta
     1921 aaaatccaaa tttacttacc tgaataacat aaaagtcatg tttttcttta atagggtcta
     1981 attcatcgcc tgcggttcct accgttagaa atactattcc atctgttggc ggatattctg
     2041 attgtgaatg atctgtaatt attggctggg gttctgcttt cttattagta gttttttcat
     2101 aattttcatt gtacaagata ggatatgttc tttcatagta ttggttatga cttgatatta
     2161 caagatcaac atcatatttt tgaaaaagtt gttcatatac atctctcagt tgttctgctt
     2221 cttgtttgtc ctgttttgtt gaataaagag gtttatgttg atgaacaact atccaatcaa
     2281 tatttggatt ctttgttgat ttttctaaat cacttttaat aaatttatat tgtttgctat
     2341 cttcctcgta aggatgttct gtagacatac ttataaagtg aacattttta aagtcatgtg
     2401 aataatatgg gttctttaac tgatgatagt taacaatctg tttatatata ttagcaaatt
     2461 cggcatcatg atttcctatt gcaattttca ttttattttt gataggctct gacattttca
     2521 tccaacaagc agcagatggg gattctttga cctgatctcc agttgtaatt attagttcag
     2581 gatgtgtatc aagaatatta tttattgttt tttttgtttc ttcgttacag taccagtctc
     2641 ctgttgctat aaagttataa tccgttaagg caagggtagc attttgttta ttgttggcat
     2701 cttcttcacc aaaaacctgt atactaataa ctaaagaaaa tataaaaaat acaggtatga
     2761 aaatagtaac cacaattttt aaaagagatt gatcattatt tttcttaatc attttaatca
     2821 cttgttaaat aaatgctata gagttgagaa cataaataaa aattttgtcg taattctata
     2881 tagtgaaaat gtttcaaaag tagaacatgt atgattaaat ttcaaaattt gagccacaca
     2941 tttcttacgg attggcttat taagcatagt gtatcaaaaa tctttaactt tctatcttaa
     3001 tctttttaaa atcagtaaaa aatgtggaaa ccagacgaga tataaaagat atatagtttt
     3061 ttcaccttct ggaaaccatc agttgttcaa attatagaaa attaaataaa ttgtcatagc
     3121 ctaaacatga tgatccccaa taatgagagc gtaaaatagt ggatgtttag attgtaacag
     3181 ggtttgccaa tggttagatt atctaagatt ttatcacaat taaataaatt taatatttat
     3241 ttattattca atttaccatt ctcaactata attataataa atttcttttt cattgtttag
     3301 tatctttata taaatagtca ttttctctcg cgagaatgaa gttatagttt attagaagaa
     3361 ataaatatat aataattgat aatgctcact tttattaaat atttagattc tatatggata
     3421 aataaaaagg aacgaagttt agagttagat aataataata atcaaaatta aatatgttct
     3481 tttaatgatt tctatatttt tttcttattg tgaacaatta ttttattgaa aacttttaga
     3541 taaaaatcaa aagagattca ataatattat acctttttgc tagtagtaat aatttatata
     3601 cagcgtctag atctttccgc ttgacttaat gttaaagttg acgacattaa tttatcaatc
     3661 tttttaatat cattttgaat tttttgcttt taaattagat attatttcaa aaattgtatg
     3721 gttcataaat atatttacat ttaatatatt atcaatttag taagaaaatt agcaattagc
     3781 aatctcatat ttggtttcag aaattgatat aattaaagag tttgctcata gcatgtagta
     3841 taaggataat aaatcagtaa acaatgctgg aattttgcta ctttattata aaatctgaaa
     3901 aacatatttt gagaagaata ttacaataaa caaatgatat agaaatagtt aaaattaata
     3961 caaattttat tgtattattt tttctatttc atttaatttg ctatttattt tattagccat
     4021 ttcttgtata tcttctatac ctgcttttcc ttgtataaga ttgcgcaatt gttctctcaa
     4081 aagtacttca gtatccttca taagtgattc atcatgtttt gctatttcac cttctaaaaa
     4141 ttcaaagtta tctaaatatg cttcgattac taaactctca gcattctgat aattgccatt
     4201 ctttgccttt tcaataattt gatctaacaa tgtgcgtata ttattaatta actctgatga
     4261 ttctgtatct gtagaagcaa gaattgcaag atcgtctgct cgtacattta gtatatttgg
     4321 tatagtattt gttatgttat taattattgt agagacttgt ctttcatcat ctctattact
     4381 aatagatgta tttaatttat aaagtgaatc tttaattttt gtaaattcat tagccattgt
     4441 ttgattcaaa ttacttgtag ttttcttaaa taattcatta gcagatgaaa caaatccttg
     4501 agcatcttga tattccgcaa tatttgtaat tgtaccatta tcaattccta tctcatattc
     4561 tgctgctgca gttttgagta aattaatcaa ggtactcata ttaaatttaa tatttgcaat
     4621 ttcattttct ggtataaccg taactagtgc tttatcgaat tgattcttta caaaagttgc
     4681 agaatccaca aattcagcag gttgagatgt aaaaactatt gatgtcagat tattcagtga
     4741 atcaattaag gttttattta atttattatc tgcagatgat aattgatctc taattaatgt
     4801 gtatatttca gttattggat gaaatgtatg tgtctgtgta agttcattat ttcctgcttg
     4861 tttatttgaa attgcagcag ataaatgacc tttgataaat tctacatttg tagcaaaatc
     4921 tacatttgta gtatttattg aggttgtgga caaggaggat gttaaattat tattcatttt
     4981 agatttgtct tcagccaatg ccattgtggg aaataagaca atcattaaaa atgcaatata
     5041 atagacatta agtattttca taattaggct gatgttatta tatatattta aaactattgt
     5101 ctattctctg gtaagaaatt tacagttaag ttaattccca ttccaactat atagtaaaac
     5161 tttcattgat attccaatat ataacctcat tttcatagtt atatattttg cagatagtta
     5221 aatttttaac tgataaatat attattagaa aggtaactat taatttcctt taatttagag
     5281 taaataacgt ttttattttc atcaatgatc ttaacaaata tattattttc aatttttatg
     5341 attcacttga gtttcctgaa gtcaagtata ttttttggta tactatcaaa gtattacatc
     5401 tattctattt aacgacaata aaaatagtat ttgttaaggg taatgattat ttttagacaa
     5461 taatatttgg tgtttaagaa ataggtaact attattattg attatcacat ttatcatttt
     5521 tgtgatcttc ctcctctgtt tttttttata aataataaat tcatattatt attttagcat
     5581 ttacttcaaa agatttctct tatggttttt ccaaaatcta ctttatacca tacatatttt
     5641 aactttcatt aatttcctca agaaaattta atagaataaa agaaataaag ttacttttta
     5701 aataattttt ctgcaatgtc taaattagat tttatagcat tatgtaatgt agtaatttcg
     5761 tccaaagact ttttatcttc aattgcgctc gcaaaatctt ctcttatcat aatttctgtt
     5821 tcttccatta gatcattatc aagttccttt aaaggatctt caatatactc ataattgtcg
     5881 atatatgcaa tatctactaa ttcagatgca ccggtataat ttcctgtatt taattctgtt
     5941 attgatttat ctagcaaatt tctaatattt gataaaatag ttaatgggtc actttcttca
     6001 ttttcttctt gtgccttaat agtatgtaag ggtgacaata aaatcatcat cattaagatt
     6061 cctgaactaa tagcatacaa actatttttt gaatttatca ttatcattaa ttgaaagttt
     6121 ttaaccatat atttcatttt tcttttctcg tatttgagaa taatatttgt ttctcttaca
     6181 atagaatttt gagaattctt taagagcaac gcattatatt tctattctat cataaaaatg
     6241 tgtatatctt ttataaaatt tggaatcaca aaagcatttg tgttcaagga tagttcttta
     6301 ctcataatta taattttacg attattcgca ctgaattttt ataagatact attaacaaat
     6361 ctaaagaaga taattcgaaa atttataaat taaattttct ttaacattat attctgattt
     6421 tttctgattt atataggata tataatagtc aattagaagt caatatcatg gagatagaag
     6481 atgagtactt tatttcaatg gatatcaata gatggtaaat tacttctatg cgcaagaata
     6541 gtaagaacat ttgcatatgg ctttttaagt gtaattcttg caatatattt gaagttaatt
     6601 ggcttcgatg atcttctgat tggcataatt ctttctgcca cattgataaa tagtgttatt
     6661 tttacgttat ttgctagttt ttatgctgat cgtttaggaa gaagaaatgt tctattatta
     6721 tatgctatta tgatgtctat ttcaggtgcc atctttttta taacagaaaa tccgctagca
     6781 cttattattg gtgccttaat agggacactt aatattacag gttcggaaac aagtgctttt
     6841 ctttctattg aacaatcaat tcttcctcaa actatcaaag ataatagaaa aagaaatact
     6901 cttttcggat tttataatat ggcaggaaca tttgcaatgg gagcaggaat tttgattgtc
     6961 aatttaccca ttctaattca aaatgaattc gaatttgatc aaatttatgc aatcaagtta
     7021 ttgtttctct tttactctct tttaggaata ttgataatag gaatttatct aaaactttca
     7081 tcttctatag aaattaaaaa agaaaaatta ttcaaaccgc tatccaaaac tttaactcca
     7141 aagtcaaaga aaattgttgc taaactatca ggattatttg ctatcgattc ctttgctgga
     7201 ggttttgcta ttcagagtat agtttcattt tggtttttta ctaaatttga tatagatctg
     7261 tcaaccattt cttatatttt ctcaataggc agtgtactta cagcattttc atatttaatt
     7321 gcggccaaga ttgcagataa gataggtttg attaatacaa tggtctttac tcatattcca
     7381 tctaacattt tattaatatt acttgctgtt gctcccaatc ttgaaatagc aattttattt
     7441 tatatggtaa gaatggcttt atctcagatg gatgtcccaa caagacaatc atatattgta
     7501 gcagtagtag aagaagatga aagaacagcc tcagcgggaa taactaactt atcaagaaat
     7561 atagcgcaag cgattagtcc atcaattaca ggttatataa ttggattttt atcattatct
     7621 gctcctttta taataggagg attgctaaag atattgtatg atataactct ttacataaac
     7681 tttagaaaaa taaaaccagc ggaggaagaa caagactaga atttggcaat agattcaaaa
     7741 aataaaggtt tcacattctt taatatccat ttttcaaaat ctattctaaa acaaatagtc
     7801 ttttatctaa ctttgttgat gtagatatag gcagtgtgaa taactgctgg gttaacaatt
     7861 agagccatac tgctattcag tactagtgag tgtggctcat ttttataata cccacttttc
     7921 aaaacagcta aatgaggaac aagctgaaat ttagctttct agttttgaaa gtactaaaat
     7981 ataaccataa agatttagct aataattctg ttcgcagata cctcattacc agaaagaaaa
     8041 aatcttcaat taacactaga aatattcata gatttttttt aaaaccatat attgttattt
     8101 ctgatttaat tgaaactaaa agcattatct aggaatcaga aattgtattt ggactataat
     8161 aattcataat agagaatata caataacaaa aaaattgaga atttcgaaaa atgttactta
     8221 ttgttattgc caaatagata ttcggaaaaa taaaaaagga atggcataag gtgaattcat
     8281 agtctagacc tatggtccac caaccccgtt gattagttca gactatgaca atttaataac
     8341 aaatgaaaaa agaaagttct ttattattac caacaaccag gaaatgtcaa agcatcatac
     8401 ttggcacaat attcttccgt ttgggaagaa ttctctgtaa atatgtcagg aactattatg
     8461 atatcttact gcaccactca tcggtacttc aatagtagca ttactgttaa gaaatataaa
     8521 attagtaata aagagaaatt aaaaatgtaa gagaggtaga tgaagattta atagaacatt
     8581 tggaaaaaaa taattccttc ttctaatgat aaatatattt ttttcaaaaa acaacaacga
     8641 taaaaaatgt taaagtaaaa gataagatac atagttacaa tgtttaacaa attattttta
     8701 ctttcacttt ttttaatact tcctttacaa acatcttttg cacagataaa tcagacctca
     8761 attgaccaag tagataatac tactactgtt acaacggagg attcaattgt aggagataaa
     8821 ttagaacaag ttcaaaatca atccaatggt gttggaaaga ttatagaaga actagttaca
     8881 gaagaaccac tagagaaaga gtagcagata aatgagttta aattccaaaa aaaatttgtt
     8941 aactggcttt tttattgtac taacgaatta gaactaaatc tttattaact agatttttga
     9001 tcggaaaaag aagattaaaa atgagtaaca ccataaatga ggaaacctgt tattatgcaa
     9061 cttatgccaa aataaatggg tatccaacag agaaagagtg ttctaaccct gctacaagac
     9121 atattgaaaa taatgctacg tctcaacaat ttcctgtatg tgaagaacat tatcaactgg
     9181 tatcgaagca gtcagaatcc taattgcaaa cttctaaatt atgctgtcat tgggtagtga
     9241 ttacactcat aggaaaacca tttcgtatcg tcctcatagt ctgaagcggc atctaataaa
     9301 ctaagaggtc tgagacctgt tttaggacta tatcgctttg ggaaccatga acctttcctt
     9361 ctgcttcggt acaaaatcag ataaaaactc gtattacttt gtatttcttt tattgatccc
     9421 tgcagtaatc ctaatgaatt tgacatcttt ggttttgatt tgcaagtgat agttttctag
     9481 tagtattata ataatatggt tgtgatgatt tgaaggttgt atattgactt tttattgcta
     9541 tttacatcta catgtttgtt taaattctta aattaactat ctatatcttg agtaatatgt
     9601 ttagatcaaa tatagttact aattacatta cattgacttc tgaagctgaa aatgatatta
     9661 taaatattac aaatgatgtg gagaggatac taatagaaag tcatttgaaa aatggtatac
     9721 tcacactttt tatagtgggt tctaccgctg caataactac catagaatat gaaccaggtc
     9781 taaaaaagga ttttcctaga ctgttagaaa gaatagcccc caaggagatt gaatacgaac
     9841 acgacaaaac atggcatgat ggcaacggtc atgcacatgt aagggcatca ttgatcggac
     9901 ctagtttaac tatacccttt attaatggtc aaatttcttt aggaacatgg cagcaaattg
     9961 tatttgtaga gatggataca agaggaagag aaaggaaaat tgttgttcaa ctaattggaa
    10021 atgactaaga taattaaagt gtagtataaa tcaaattaat tgtacagatc aatatatgaa
    10081 tcatggttct gctcaaaaaa ggtacattgc taataattaa aaaactttgt gtaaaaattt
    10141 tatttagatg tttagatttt tacaatacca aaaagagagc gttgaaaata aacaataaaa
    10201 tgctagaacc ctaaaaaaat ttcgtcaatc attaaaattc ttttgtgaac tgattccttt
    10261 cttactttgt attatcattt gtttattaaa tattttttat tcagatgtgt ttcattccaa
    10321 tgtttcataa agtagtcaat taaaggctca aaatcgcttc ttattaaagc ttctttttta
    10381 tttttattct ttgaaatctt tgcaaatttg tgcgcatatt ttatacacgt ccagcatcca
    10441 acaagcggaa ttaaaagggc gaaataatat ccattatatc ttcctgaaaa tttccacgct
    10501 tcacctacaa tacaatactt tgaatccctc tcaatcagga atttgtctac tctatctaat
    10561 cctaaagata attttttaga ccaaatagga cataattcgt taaatttaat gggtttcttt
    10621 acaatgtttc ttgatgataa ctcccattgc ttattttttc ctaagccagc attattttta
    10681 tcattactgt tactatgaga aaaaacagta ttagataaac tatcccgtat catcatattg
    10741 tataattgaa aacatatact ataacgattt ataattttgc tgtctcaatt ttagtttctt
    10801 tacacaacct tttgaggttt tattacgata acttatttta atatttacaa tgtcaaataa
    10861 tatttaatag atataatgct aactaatacg caaaaaatct ttaatttgcc acaaaagaca
    10921 tcccactaaa gacatctaaa tgaaagaagg gagtttagga taacaaaaaa ttattttctt
    10981 ttttgtgtct ttataaataa atttattatt tattatttat ttataatttt tccttattga
    11041 aataatattc atataactga gataaaaata atcttcaaca cgtctctcac attatttatc
    11101 taataaacaa catatataat ctaggtctag ttcaactatc gaataggcta tctgtaacaa
    11161 ttttgaaatt attattgtat cattttagat cttactgata aagggtataa aaaactttgt
    11221 tgatataaat tatttgtagt acatattagt atacattcgt cgaatactcc acaaacttta
    11281 ttgcttttca tttttaataa ttctttgttg tattggtagc acctactcca atatgatttc
    11341 gtaatagcca gacaagcatt aagtccaagt ccattataat gtaacagttg agtaaaagaa
    11401 aaaaattatt tttactcaac tattacatga aataaaaata gaattatatc tatagttagt
    11461 atgaattgat ttacagtgtg agaataatga taatcatttg agaagatata cttttttatt
    11521 aatttttatc tccattttta aatatgggtc tcttgtacac attaaaaatc aatatatatt
    11581 agaattaatt cagttttaaa aataagagga tttaagtgaa gataggaggc tagtatttat
    11641 tcttctccat atgacgcata ttcgtttcca tattcgccat tatcatcagt gtattcatat
    11701 ctgtattggt aatcatagtt gttgtcgttg ttgtcatagt ttttgtcgta ctccttacta
    11761 tcgtcttttt tatcattctt tttatcatct ctgccattat ccctcttgtc atcatagttg
    11821 ttgttgttgt tattgttgtt gtcatattgt ttgtaatcct cttttacgta accatagtcg
    11881 ttgttgttgt tgtcgtattg ctgctgctgc tgctgataat tgccatatcc atcatcatat
    11941 tgatcatatt gaggaggtgg ataattgtta taattattat caggatactt gctagctggt
    12001 gggaagaaaa tactttttat attttgtgga caaccataac ttccattcca accatcagta
    12061 tcaaatttag tcaaatcttc tcctgggatg ctacagaatt ttgttgcatt ttctaatggc
    12121 acttgtgcaa catcataata gttaattgta cctcttattg tatatccata aatgccagca
    12181 tcaataacct gtctattata gaaggtacca ggttgaccaa atactgcact gattggtaca
    12241 tttagttcaa tagcatctgc atcttcaaga ctttctgcac gttgaaaact tgctatgttt
    12301 ttgaaatagt atttatctgc aaaaagtgtg gcattaggaa ttggtaatgt agttgcatca
    12361 tctgataaat caacttctag attatgtttt ccatcatgaa tgcctggtaa cttgccataa
    12421 gcaggttcat ttgtatgtcc taatactact ctaatatcat ttgcatgctc ttcctcttta
    12481 ggatctatta taagacttgc atgaggaaat gccatagctt catctattga ataagaactt
    12541 gcagaaacga tggcgattcc agcaaggatt attatcaaca ttgtcggtga agacttggat
    12601 aatttattat tcataccaaa acatttcatt ccagagttaa aaatagatgg ttacattttg
    12661 tataataata tgaatataaa atagacaaat aaatattatc tagaaatttt ctctaaatat
    12721 aaataactat cgtagtatac tattgatgta tcatatttct tttatattct attatcattt
    12781 ttaaaatcat gttaccacaa aaatctacaa tattagcagt tatgatggca acactattga
    12841 gtttaggatc attgtctttg atgcctttag atgtatatgc aagccatagc tatgaagcag
    12901 gagttcaagg agatgctata ccagataata cacatatcga tttgagtcaa ttaactctac
    12961 catcagcagg agtgttcccg ctatatgact ctagtcctaa ttttgtttct ggacattttc
    13021 tatatagagc tccctgtgac ccaggtacaa agataccaat agtatctgta attgcaggtc
    13081 atatcgacga atcagcaaat ctaacacatg ttgactttgt tccattatat tatgttggtc
    13141 atgcgtcccc tctaccaggt atctgtgtat atcatgcaca tcttcctgat cctctcgaag
    13201 gtggctcacc aagagttaca gatgtcgact tggtaaactt ctcacctcga aatgttacat
    13261 ttaatcctgg agatgcagta gatgtgaatg ttcagcgaac actaggcagt ataggtaatt
    13321 attatgcaaa cgatgtaaaa ttaccacttg agttagcagg aggaaataac aaagttctag
    13381 atttgaatgc acacgtagca gcaacggcaa ataatgtgaa cgctcaacaa actctaagca
    13441 acttagtaca gctcaatgct gactatatga aatcaccatt tgagttcacg ggtggaaata
    13501 accaaatatt aaacttgaat gaagaaaatg acaacaacaa caatgacaac aacaatgaca
    13561 acaacaatga caacaacaat gacaatgagg atgagaatga caatgacaac gaagatcaag
    13621 aggatgaata gacaagtaat aatttaaaaa ctcaaaattt tttttacttg taaatatttt
    13681 aagttaatat tgatcccaac ttttatatat tttctataga tgatttttat ggtcatataa
    13741 taatagtaga taatattgta cattcatcga atgtaccaca agattattaa tgttcaaata
    13801 ataaagttaa acagacaaat ggataaataa attatccggt tgtttagtag tttcatttca
    13861 tgagtgaccc aataagggtc acttttatat attactagaa taattgtaga ataaatcaaa
    13921 tgattttctt cgataaacat atataattgg gttatatact cctacagatt ctttgtttca
    13981 aaaattgtaa tttataataa tacctcaata gattcaatct tagaataaac tgttggagtt
    14041 ttgtatggat gtacttaaga aaactatgag tggtagaatg atgatgatga tgatgcaagc
    14101 cgagatacta aattcagaag acctaaaaga aagagataga tagcttccaa attgttagtc
    14161 tagctcacta gacaaaaaac catacaaata aattaaaagc tcttattaga gaaagaaaaa
    14221 aaatttattc taaaccgaga agatttagta ttgaaatgag atagtttatc ttatagttgg
    14281 tttaatccaa tatttcatca actattaaaa gttatttttt gataaaaata agtcataaat
    14341 attaattcta aaaccaacag ttttttatct tactatattg tattttacat accaaaacct
    14401 gagctctcta ttgtcatctt ttctgggtgt tggaggtttt gtctctatag cctttgacac
    14461 ccatatttat ttaccaacaa tcaggcgatg ctaaaaatat actttgtaca gcattcttct
    14521 ttattggaag attcaactgt cattgcatca tggactagaa tgaaaacgaa cagtgcaata
    14581 aaaaatagtc tgaaacctcc aaaaatccat tttaatgctt ttgatacaac gtttgtagct
    14641 atactcttaa gaacattaac tgagactgga tcctgttcta tataattaat atagaacgtg
    14701 tattttttga aatatgaatg ctgagtttgc gaagattgtt attgtcttgt aatactcttt
    14761 tttcaaaaat ccaactgcca gacttgataa gttaataatt ttaggtttag aaaactcttt
    14821 tgattttaaa aacgaaatta atgtactact agaattcgtt aatcatatct acataatcct
    14881 caacaatctt ttctgtaaaa ctacgtgtgt atttagatag tagatcttga agtattttcc
    14941 tatccattcc aatttcataa taattaaatt cattctgttt acgaaccttg acaatattag
    15001 cagcttttaa tcttcccatg tgccacgata tagtagaagg aacttttctt gcatgtgtca
    15061 ggatatcgtt aaatccacat gctccatttt ctagcatata taagataatt tttcgagtag
    15121 tttcttgtcg caaaagccca attacataag attcatggct ggaaacatca taagaaaaat
    15181 accttgtaac tctattatta actctattta ctcgaatctt tccagaatta tcaagggatc
    15241 ttaaatgata agataaaacc ccattagaaa ggccagtctt tcttaacagt tctctgtatc
    15301 ttattccagg aatttcatta acatatttaa ctattttatc ttgacttgaa tttgtttttc
    15361 tcttatgatt aattgtagac atactttata cctgcttcac ctgaggactt ttcaaaaaag
    15421 aaataccaaa gaatataatc attattaaca agatgaaatg agaaagctca atattaacct
    15481 gaggaataaa cattgcatga aaatcttctg tcgaattcaa taaatataca tattcggcaa
    15541 taactaaagt cacaaatccc aaagtcataa acaagatacg agttgttctg gatttgagat
    15601 atgccattat agatacaaca gcaagaaatt gagatattat caatgcaaca agatgcatta
    15661 gaatatggta tatcatatgt tggtgaaata agtgaggtag aataaacgga acgacagaaa
    15721 gagaaataac aatacttgcg attaatacaa gtaatcgagg tttattataa aatgttccag
    15781 gactattgta attccttaac aacaaagaaa aataccaata attgacataa atgtattccg
    15841 gtacaacttt agaatttaag gtcaaattgt tagtagtcta taatataaca gatatagaat
    15901 aaataaccat tttctggagg agtattattc gaagcttgta ctacaaagta taaaagtaaa
    15961 aaattataag aaaatttaat gcaaaatata aaaaaataca gcattattgc gattttaact
    16021 ctatcggtgg tagcaggaat ttttagtaat attccactat catacgcaca aaatgacaca
    16081 atgtcaatgt caaatatgac agaagaggat gctactacta ctgaggcagg ccattccgct
    16141 gaagaatatg aaaaagaata taatgaaacc agtactgtga gagattcagt tacagaatta
    16201 ttacaagata aggttattcc aggaggagat tttttgcatc tttatgattc tacaccatac
    16261 cacattttga atggacatgt tgctttaaaa gctccttgtg gagatgatag tactactcca
    16321 attcaaatct tgattggttc tgctccaaac atgacagtaa caactcttga gaatatagct
    16381 ccgctatcaa ctccaggaga acaatgtcta tatcatgctg atttgatacc tagcggcaat
    16441 gtaacaataa taacagatat tgcattaaag aatcctacag aagaagatat ggaatttcca
    16501 ccaactgcaa cagttgtagt aggcataaat gaaataacta aaggagagca tggacattct
    16561 gaaagtgaag aagaacatgc tgctgctaca gaggcaggac agacagaata agaagaatta
    16621 atagcaacat caaaaaaaat aattttcctt tttttattca attttcttag gctttaaaat
    16681 gttaaataaa aataaaatta tctaaattac tcttttgcta ttagtaggat tattatttcc
    16741 tctctcaata aattctgtct catcaaagga tgaggaattt gttgaaatac cagcagaaga
    16801 atattatgac cctgccgatt atggattaaa accttattca tcacctggaa ctggataagt
    16861 tgaaaatgaa aatggatttt acaaggaaaa tgctgagcag tttgcaaatt caaaaaatta
    16921 aatctttaga tacaataact ttagtaacat attaaatatt cttatttgag agtattgaat
    16981 cttctttttc ccattgcata tgacaagcag atataagtat aattcttaat aataatatca
    17041 atctttccaa ataaattaaa ataattttaa atttcaaact tcctaagtga aaattaaatc
    17101 aaatctttat cctgtcataa ttatcagtat ttgaaattga tacataaata taaagttgcc
    17161 aaaaattcga atgaaaagat caacaaatac caagaggtcc acatgaaata tatctgtcta
    17221 aaagatttag gaacacaaaa gaagtattta atcactatta cattaaaatc ccttttaatt
    17281 gcttataata attataatgc ttatcttgta aataatataa aaaaaagaaa taacctttac
    17341 tttttagatt cttagagaat tactttgtat ggatagagaa gaatcaaatt gaggagtaat
    17401 gattttaata tatctatgtt ttgtaatata taatgaacat agaaaaatca ttgtttctat
    17461 taggcttaat atcattgatg tcaatttttt tagtaattaa ttcgtataat gtacaagggg
    17521 cagaggaata tcaatttaaa attaaatttg gttcgaaagg agttgataat ggtaagttta
    17581 ttcagccgat aggcattgca gtagataaag aagataacat atatgtagga gattttgctc
    17641 gtcattcaaa tatcattcag aaattttcag ataatggaac ttttatcagt tctgggggga
    17701 cattaggaaa cagtccagga ttttttacta accccgtgag tttagaaatt gatagaaata
    17761 atggattttt gtatatatca gattttggaa atcctaattt tgcaattcag aaattcgcac
    17821 ttaatggtac ttttttgaaa tcttttggtg aatttggact tggagatgga aagtttatta
    17881 atccgggggg aattggactg gattctcaag gtaatgtgta tgtaacagac tttggtgaaa
    17941 atagtaatgt tcagaaattt gataagaatg ggaacttttt gcagagtatt ggatcccatg
    18001 gcattaaaca aggacaattt aacaacccca cagatgttgc ggttgattct ataggcaata
    18061 tttttgtaac agattcaggt aatgaaagaa ttcagaaatt tgataagaat gggaactttg
    18121 taatgacatt tggaactttt ggacataatg accgccaatt tgacaactta ttaggaatag
    18181 aaatagatga aaatgataat atttttgtta cagatagtga gaatcataga atacaaaaac
    18241 tcaataaaga tggagtattt cttacttcat ttggcaaatt tggatcagga gatggagaat
    18301 ttatccaccc atcgggaata gacatagatt cgaaaggaaa cgtgtacgta acagatgaaa
    18361 gaaattatag agtccaaata tttagtccag taacatctta ttttaattag ggtcaaccaa
    18421 ttttacggtt tatccgataa atctaaaatc ccttcttcac tttttattaa agaatataaa
    18481 attagagaca aaaatacgtc aactcatcaa tgtacactac tattattagt gcatgttatt
    18541 agtaatccac accattgtat actattagtt atatgctatc atgtatacta attatatata
    18601 tatgtcaaca aaaagacacg atcgtaaaga agaagagtca aatgaaacac attcaacaac
    18661 aacaacagag caacaatttc aacgagaaca acagcaagca gttaataaag cattagatga
    18721 aactagagac aatatcaaga aaacaactaa tgaagcaaga aaagaaattc cacgttatac
    18781 acaaatcgtt aacgattatc aggaaaatac aatacaagct attagagaaa ttacagataa
    18841 ttatgtagaa tctcaaaagg acattatagt t
//
LOCUS       DASVEV010000118         4380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_41097, whole genome shotgun sequence.
ACCESSION   DASVEV010000118 DASVEV010000000
VERSION     DASVEV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41097"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..868
                     /locus_tag="VF222_05375"
     CDS             350..868
                     /locus_tag="VF222_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7141891.1"
                     /translation="MTMPKGWKNEQTTTSKKENFNEQKEQSSSKNEQTSSTIITVDDK
                     IIIQELIAKHNIALDNKNIDEWTNSWTDDGVWSTPFGEAKGKTELKNMINQVTNEFAS
                     GKRHLSTNIIIEDAPNNMASAQSYLIVTEAKKTPEVVASGTYIDTLKKDANDKWKFVQ
                     RRLEIDLVTDSN"
     gene            complement(936..1109)
                     /locus_tag="VF222_05380"
     CDS             complement(936..1109)
                     /locus_tag="VF222_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141892.1"
                     /translation="MPNLDPWVNQNEKHMATKTITNKFENHLLSIKAEHQKRIRPNLH
                     PTYNERHQKIIER"
     gene            complement(1688..2002)
                     /locus_tag="VF222_05385"
     CDS             complement(1688..2002)
                     /locus_tag="VF222_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141893.1"
                     /translation="MSTSINWTELVRDRKGVRTKDNNPCGNIIGNDEKNIIIEDGAMR
                     QHIYKVPKSSIESYNGAELRLNISYGELETDEVKDDDKGMLESITDSVKDKTKSLKKH
                     ID"
     gene            2852..3469
                     /locus_tag="VF222_05390"
     CDS             2852..3469
                     /locus_tag="VF222_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141894.1"
                     /translation="MSTRKEEKTNETIAVNEPKNTTKFNKDNNNFNERTNNTLNQQQD
                     AINKTLDNTLQNVKRTTDEATREIPRYTQRIAEYQQQTIQTIKDIASDFIEAEKEAIG
                     SFQSQVDRNSSGNVNSSSNGLWDMYNPQRIAENYAVMVNNFTSYLLNTSNLINNALAS
                     NMRVYNTALQQTRDNVKACAKTNTNYIKAVEEQSVNSYNKRVNTN"
     gene            3610..4008
                     /locus_tag="VF222_05395"
     CDS             3610..4008
                     /locus_tag="VF222_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7141895.1"
                     /translation="MAEPNSDIQFLYFLFTKQYGFSISDVKVVQNDNKCLEIIHSSRE
                     DNNNDNNNDDYDIFIVDTHLRDTSGFEVARKIRNRLPHKRIILTTTNSLDNISSMIDS
                     IGIKSQDVLFKPFSFSDLVSILKEPGMSYK"
BASE COUNT         1685 a          649 c          634 g         1412 t
ORIGIN      
        1 tgtagttttc tcgggttcac tatatttcat taacatagag tttatctttt cattcccctc
       61 agaaactata attttaataa taatgcttaa atgagttctg taaacatata gctgaattgt
      121 attgcttcca aaaagcgtcg gataaatgta gctaaaataa ttctataata aataaactta
      181 aacagttttt aatctatctt aggcgatatt atttgctcaa tatttactgt gggcttcaat
      241 tacctggatg ttaatgacca ccttgacatt catttttaat acaattcgtc aggtaggatt
      301 aatgataata gtcttttaaa ttaaatatca gttgtattaa ttggaaccta tgacaatgcc
      361 taaaggatgg aaaaatgaac aaactaccac ttctaaaaaa gaaaatttta acgaacagaa
      421 agaacaaagt agttcaaaga atgaacaaac aagttcaaca ataataactg ttgatgataa
      481 aataattatt caagaattaa ttgctaaaca taacattgct cttgataata aaaatataga
      541 tgagtggact aacagttgga cagatgatgg agtatggagc acaccttttg gtgaagcaaa
      601 agggaaaaca gaactaaaaa atatgattaa ccaagttact aacgaatttg cgagtggtaa
      661 aagacattta tcaacaaaca ttataatcga agatgctcca aataatatgg ctagcgcaca
      721 atcgtaccta atagtgacag aagcaaaaaa aactcccgaa gtagtggcgt ctggcactta
      781 tattgataca ttaaagaagg atgcaaatga taaatggaaa tttgtacaac gtagattaga
      841 aattgacctc gtaacggatt caaattaata taaagagagt ctaatgactc ttttatctga
      901 tactaaatca aaaatttaaa taaaattgta tttttttacc tttctataat cttttgatga
      961 cgttcattat aggtaggatg taaattgggt cgtatccttt tttgatgttc agctttaata
     1021 gatagcaaat gattttcaaa tttgtttgtt attgtctttg tagccatatg tttttcattt
     1081 tgatttaccc atgggtcaag atttggcata atgcataaac aaaaaaggta tttttcaatt
     1141 tgatagctac attaagagag ggagagagat gttaaaggag acttgtaaag atacaaacgt
     1201 ttagttgtgt caatccgagt aattgagatt ataagaaaaa taacaagtct tctagtgcct
     1261 aattaccggt aaagaacaaa taaagatatt aactatattg tctaaattta tatcaagagg
     1321 ttctgtttgg agttctttgg actgaaaaac caaactagag gacatgttgt atacattcat
     1381 gagcctcaat gaacctcctg ttttgttact ctgatagtcc aactatatat ctctgatgta
     1441 ttaagaaaaa attattaatt gttaaatcaa tttaagataa atttgatgat aataaaagct
     1501 aaatataaca ttgtaatagc accctactac atgtggcgct atactcccga ccatcttgct
     1561 ggatatgtgt atgaatccaa ctaataatat tttttaaatt taataatatt taaaatgaat
     1621 aattaaaagg aaatttttta gaataataat aatagatata ataattcact gattatcata
     1681 atctatttta gtctatatgt ttttttagcg atttagtttt atctttgact gagtctgtaa
     1741 tcgattccaa cataccctta tcatcatctt ttacttcatc agtttcgagt tcaccatatg
     1801 aaatgttcaa tcttaattca gcaccattgt agctttctat ggatgatttt ggaaccttgt
     1861 aaatgtgttg acgcattgca ccatcttcga ttattatatt tttttcatca tttccaatta
     1921 tatttccaca tgggttattg tctttagttc tgactccttt tctatctcta actaattcag
     1981 tccagttaat tgaggttgac atattaaatc cttatatttt gtaatatata gccatttcaa
     2041 tcataactat taagataaat taaagtgacg agattttttt tgttgtactg aagatatttc
     2101 atattcggaa tcttaagtgt ttcaaatatg ggaaaatatg gatacacacc aatcttgtaa
     2161 atttaaaaaa gttaagggag gaatttataa tatttagaaa attcaaactg aaagagagaa
     2221 aaatagtgat caaaccgaaa gagaagaaat gagggaggag actcatcatc tagaggatga
     2281 taaacaggag gaggaagatg ataaactgga agaagagcaa tatgaagatg aataacccgt
     2341 caatgtttaa ctaaaaccta gtccggctaa gacaatagag atttcttgta gtgttttaca
     2401 tacgtctctt ttcactcatt tcacctacct gccctttcaa tttcctactt aggtactact
     2461 atttagaatg ttttccttta aaaagtactt gatttattac gaaaacattt attagataac
     2521 agttttcggc atcaaaattg ttgaaaaaat caacactctt ttattctacc ttatattgtc
     2581 ataatgcacc atcattcaat caagaaatct gcttgagcat tatgttagtg ggtgttaagc
     2641 taggcaattc taacacccac ttttcaaaac agctatatgt tgtcaatcat aatcattggt
     2701 tttagctgaa gcctctctct acttgttatg attgtaatta taaatatatt accttcaatt
     2761 atacaattaa tatatcaaat attaataaaa tatgactatt aaaaagacac tgttacataa
     2821 gtttatatac tttagtaacg tatatatata catgtcaaca agaaaagaag aaaaaacgaa
     2881 tgaaactata gctgtaaatg aaccaaaaaa tacaactaaa tttaacaaag ataacaataa
     2941 tttcaatgaa agaacaaata acaccttaaa tcaacaacaa gatgcaataa acaagacact
     3001 agacaacact ttacagaatg taaaaagaac aactgatgaa gctacacgag agattccaag
     3061 atatacacag aggattgctg aatatcaaca acaaactatt caaactataa aagatatagc
     3121 ttctgacttt attgaagcag aaaaagaagc aataggctct tttcaatcac aagtagatag
     3181 aaatagtagt ggtaatgtaa actcctcatc caacgggtta tgggatatgt ataatccaca
     3241 aagaatagca gaaaattatg cagtaatggt aaacaacttt acaagttacc tattaaatac
     3301 tagcaatcta ataaacaatg ctctagcatc aaatatgaga gtatacaata cagcacttca
     3361 acaaacacgt gataatgtga aggcttgcgc aaaaacaaac accaattaca taaaagcagt
     3421 agaagaacaa tcagttaata gctacaataa aagggtaaat actaactagt acttaacagc
     3481 tatttattat tatcatcagg tacaacagac agtatctgaa gaactagcac accatacaat
     3541 aatgattgat ttaaatcaat ccacaacaga aaaccaaaaa aagaaagaaa aaaacctaaa
     3601 cgaatcctta tagccgaacc aaactcagat attcagtttc tttattttct ttttacaaaa
     3661 caatatggtt tttcaatatc cgatgtgaag gtggtacaaa atgacaacaa atgcttagaa
     3721 attatacatt ctagtagaga agacaataac aacgataaca acaacgacga ctatgatatc
     3781 tttatagtag acacgcatct cagggatact tcaggctttg aagttgctag aaagattcgt
     3841 aatagattac cacataaaag aataatatta actacaacca attcattaga taatataagc
     3901 agtatgatag attcaattgg tataaaaagc caagatgtgc tttttaagcc ttttagtttt
     3961 tctgacttag tttctatttt aaaagaacca gggatgtcat acaaataatc ttattaacaa
     4021 aactttttca tgttaccata ctagatgtga ccgtcccaga tgttgccatt aaactactaa
     4081 ttttattctc taaattttgt tgtgtttatt attctatgtt atcatatggt ttgaattggt
     4141 caaggagttt cttattagaa aattaatctg acattattaa aataagtttt tattactatt
     4201 gaaaggtaaa ctaaataact atgtctaatg gtacaagttc aaaaagttct tctaaaaagc
     4261 ctactaaaaa aatcacaaca gcagcgaagg aactaaaaga aacaaaatca gttcctatca
     4321 ccgtagaatt aaacatactt aaagttctga taaatgaacg tcctcttatt ccattattta
//
LOCUS       DASVEV010000119         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_41199, whole genome shotgun sequence.
ACCESSION   DASVEV010000119 DASVEV010000000
VERSION     DASVEV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41199"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1739
                     /locus_tag="VF222_05400"
     CDS             378..1739
                     /locus_tag="VF222_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141896.1"
                     /translation="MNKNNYSYRNYFIFGIVIPLIFTLCIFVGNDLDNDAFGVEKEPP
                     MVYDQNLQITQLTDKLQFPTGIDFLGENDILVIEKNTGQVKRILNGEILNEPVLDVNV
                     ASVSERGLLGIAILNAKVVTNQYPTTNEESQENNLNNEIKVFLFYTETKDIDNGDILG
                     NRLYKYDFIDGKLVNPQQLLDLPFLPGPSHNGGVLDLGPDNNLYLVVGDINREANLEY
                     MTLAQNIEDGQVPDGRGGVLRLTVDGDPIIDDDGFSLGDSGLLNFYYGYGIRNSFGIG
                     FDPVTGILWETENGSNYGDEINVILPGFNGGWRQVLGLSTEYENFKNKEFDKSKLILF
                     NGKGTYYEPVFTWVDTIAPTAITFINSKIFGEEYENDLLVGSVKQGRIFHFNLNETRT
                     GLDLPPEQLVDKMADVDEELEPVTLGRNFGIITDIEINPYDGKIYVVDGQSKAGKIYQ
                     IFK"
     gene            2202..>2624
                     /locus_tag="VF222_05405"
     CDS             2202..>2624
                     /locus_tag="VF222_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141897.1"
                     /translation="MKYQNIVIYLIIPLALLSLPSLLENVQAQTNSTNQIAPQQNQNM
                     NLTTQALMKVNIFELKDALMKANLAIVDGNLKEALTGVREIESQLLQIEPMPTKFLDN
                     IHKAINAIARSDIDKALNTLTGIQITIIKAENQIFKAAV"
BASE COUNT          989 a          341 c          411 g          883 t
ORIGIN      
        1 tttttaatat gtaatgaggt aatttgattt atacagattg taaagtatct attgaaatct
       61 aaaagatata aaaaaaaata ttctaaaact atggagatct atcgatttaa ctggtcccac
      121 cttatagaag ggttcaaatc ccattggccc cattctataa gtgggttcat atactttgaa
      181 tatgatctta aacaaataat aaaaaagcgt taataatttt atgcgtatag ttttattatc
      241 tatttataga atttatactt aaattcaaaa attttcgaat taataattac aggtttcatc
      301 attaatttgt atttcaagtc taaagaattg aactaacatt tttaaatcaa aaatttataa
      361 ataattcttg taacagattg aataaaaata actattctta tcgtaattat tttatttttg
      421 gaattgttat tcccttaatt tttactctgt gtatttttgt aggtaatgat ttagataatg
      481 atgcgtttgg ggttgagaaa gaaccaccaa tggtctatga ccaaaatcta caaataactc
      541 aacttacaga taaactacaa tttccaactg ggatagattt tctaggggaa aatgatattt
      601 tagtaattga aaagaataca ggacaggtta aaagaatact taatggtgaa atcttgaatg
      661 agcctgtttt ggatgtgaat gttgcttcag taagtgagcg tgggttatta ggtatagcaa
      721 ttttgaatgc aaaagttgtt acaaaccaat atccaactac taatgaggaa tctcaagaga
      781 ataatttgaa taatgaaata aaagtatttt tattctatac agaaacaaag gatattgata
      841 atggcgatat cttgggaaat agactataca aatatgattt cattgatgga aaattagtta
      901 acccacaaca gctattagat ttaccttttt taccaggacc atcacataac ggtggagttc
      961 tagatttagg gccagacaat aatttgtacc ttgttgtagg agacattaat cgtgaagcca
     1021 atctagaata catgacttta gcacaaaata ttgaagatgg tcaagtacct gatggacgtg
     1081 gaggagttct gcgtctaaca gtagatggtg atccaattat agacgatgat ggtttttcgt
     1141 taggagattc tggcctttta aatttctatt atggatatgg aataagaaat agttttggta
     1201 ttggttttga tccagtaacc ggtattttgt gggaaacaga aaacgggtct aactatggag
     1261 atgaaattaa tgtaattcta ccaggattca atggtggttg gagacaagtt ttgggattat
     1321 ctacagaata tgaaaatttc aaaaataaag aatttgataa aagcaagtta attcttttta
     1381 acggaaaagg aacatattat gaacctgtat ttacgtgggt agatactata gctccaacag
     1441 caattacatt tataaattca aaaatttttg gtgaagaata cgaaaatgat ctcttggtag
     1501 gttcagtaaa acaagggcga atatttcatt ttaatctaaa tgaaactaga acaggattgg
     1561 atctgcctcc agaacaattg gtagataaaa tggcagatgt tgatgaagaa ttagaaccag
     1621 ttacattagg gaggaatttt gggataatta ctgatataga aataaatcca tatgatggta
     1681 aaatttatgt agttgatgga caatcaaaag ctggtaagat ttatcaaata tttaagtaat
     1741 ttgcaaatat tatgtaacca atgtaaaaac catattatca ttattactaa tgataatata
     1801 catttaatga tttatcttct ggagttgcat tcgcaattat aggaatagaa gcttcaatca
     1861 cagtcattat aaatgctttg acaactttca atttcattaa agcttacttt tttatacctg
     1921 aaaagtttta gccaaactta actttctaga acctagacta atatatgatc aatattgata
     1981 acagtggaaa tataaagatc tattttgtat tatataccta gacaaaaatc tttactactt
     2041 ctatactagt agaataatcc ttacttttat ttaattttta aaatatttta atctataaat
     2101 aaacttgggt tatataattt atgtagtaat attctagaaa aacaatttat aaatgatttt
     2161 attcataact catataaaca atctcctaca tttttattca aatgaaatat caaaatattg
     2221 ttatctattt gataattcca cttgctctct tatctcttcc gtcattgcta gaaaacgtac
     2281 aagctcagac caattcaacc aaccaaatag caccacaaca aaatcaaaat atgaatctca
     2341 ccactcaggc attaatgaaa gtaaatatct ttgaattaaa ggatgctttg atgaaagcaa
     2401 atttagcaat agttgatgga aatctcaaag aagcactaac aggtgttaga gagattgaat
     2461 cacaattgtt acaaatagaa cctatgccca ctaaatttct agataatata cataaagcta
     2521 tcaatgccat agctagatca gacattgata aagcattgaa tactcttact ggaattcaaa
     2581 ttactataat aaaagcagag aaccaaatat tcaaagctgc agtg
//
LOCUS       DASVEV010000120         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1401615, whole genome shotgun sequence.
ACCESSION   DASVEV010000120 DASVEV010000000
VERSION     DASVEV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401615"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF222_05410"
     CDS             <1..198
                     /locus_tag="VF222_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141898.1"
                     /translation="DAIKFLQRSIGLEKSNLGIPKGLINDLCKGFKIYKINKNRLKNI
                     DNQRLIHDFLFKDMILFESAN"
     gene            182..946
                     /locus_tag="VF222_05415"
     CDS             182..946
                     /locus_tag="VF222_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141899.1"
                     /translation="MKVQISSPVISKLISYPKLDLYEYHKRISDLYKIGVKDAILEGP
                     TRVCELKILGKGHSGIVLKVNGYSEKNMALKIRRLDSRRKDSLNEVKNQKLANLIEIG
                     PQIIDNTDDLILMELITGKGISDWLDDPSNFNLDNSKKIITMVNEILEQCYRLDVLNL
                     DHGELTRIDNHVMVSEYNQISIIDFESSSTKRKPSNVTSASQALLLSGGLISKKICKF
                     IKIKDHKYLLNSLINYKKRKTRQNFEAILALVNGKR"
     gene            1043..1351
                     /locus_tag="VF222_05420"
     CDS             1043..1351
                     /locus_tag="VF222_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141900.1"
                     /translation="MTEKSGSIESSNDTIPFPTLTILYLPADASEVVEEVSQKYYNMT
                     SEDCVGYFHDGVKREYKKLTIWSQGIDSLWFNAIIARIKEVAKVEQVPIVSGGIMYTI
                     "
     gene            1461..1793
                     /locus_tag="VF222_05425"
     CDS             1461..1793
                     /locus_tag="VF222_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141901.1"
                     /translation="MENITTTTTVFKIKQLVDNGSNVTLSLSEDVELETVSQQQMILE
                     AVDRTVTDDEVKQQIKPLLEAILRSQPQTVIKTYPKTIIQITMPKRRYEMMGNPQVGE
                     TLQVDIKK"
     gene            1943..3262
                     /locus_tag="VF222_05430"
     CDS             1943..3262
                     /locus_tag="VF222_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7141902.1"
                     /translation="MQGKKFDIIIIGGGILGVSIGYFLTVNSDANVLIIEREKNVSSH
                     TSSRNTGKVHAPFLYDPIKKKIFARISFLGYNMWETYSNRKMMHFKQDGVLEIAKEEK
                     DIKVLKKYVEWGIKNGLEEKDILFLNDQETRTIEPNVRCYGSIYCKRDASVDYGLLTT
                     TLKNDYLLAGGKLLNLHRVIGIKHQKDNCSIEMVTKDRERIIIDSKYLINTAGGHSLD
                     IAHQFEIGLDLFDLHFRGEYWLAPHEYEKLTTHSIYSVPKQQNFPFLDPHWIIKADGH
                     CEIGPNAVPVFGPYSYNLIDNFKNFLPKTKEILFKKGLWSLFYNREFLSLLTNEIAGS
                     LSKRIMIQRVKQFLPALDTKKFNTRGTAGIRSLLIDEKGNFIPDTLLLKNSLSLHILN
                     YNSPGATGALPLSAMIVQELANEKLINKITKNNQLWNIEDIYSKIKF"
     gene            3410..3483
                     /locus_tag="VF222_05435"
     tRNA            3410..3483
                     /locus_tag="VF222_05435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Pro,seq:cgg)
     gene            complement(3906..>4950)
                     /locus_tag="VF222_05440"
     CDS             complement(3906..>4950)
                     /locus_tag="VF222_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141903.1"
                     /translation="KVFESFVRFYSLPSGNEFDPTLIFGLIFPIFYGLMIGDVGYGLV
                     ILLVSLWVIRRVQDKKSNLTIMPKFLRNFAKTILRPSQMVKLAKAMIPGCIIAIVLGF
                     CFDLYFGFHLNGYLFSYLNNQFGLNLPSDGAFLNPIGTFGLRKLLLFSGYVGLGMVSF
                     GLVLGILNSMREHQIKHIISKVGWLLFGWGIALVGLAMINHENINPLQNVQGVGYFVL
                     LFGGIGMMFYGEGLRALMELPSIISHILSYTRIVGILLASVILAHVIDFIFLKSLDNS
                     IALSILGIMIFLIGHTFNIIIGVFEPGIQGARLIYVEFFSKFYHGAGRPFKPFGNKRR
                     FTTGGYDIEMSKK"
BASE COUNT         1912 a          760 c          751 g         1527 t
ORIGIN      
        1 gatgcaatca aattcttgca acgatcaatt ggattagaga aaagtaattt aggaatcccc
       61 aaagggttga tcaatgattt atgcaaagga tttaaaatat ataaaataaa caaaaataga
      121 ttaaaaaata tagataacca gcggctcatt cacgattttt tatttaaaga tatgatttta
      181 tttgaaagtg caaattagct caccagtaat ttcaaagtta atttcatatc caaaattaga
      241 tctgtatgaa taccacaaga gaataagtga tttatacaag attggagtta aagacgctat
      301 cttagaaggt cctactaggg tatgtgaatt aaagattttg ggaaaagggc attccggtat
      361 agttttgaaa gttaatgggt attctgaaaa aaatatggca ttaaaaataa gaagactaga
      421 ctcccgtaga aaagactctt tgaatgaggt caaaaatcag aaattagcta atctaataga
      481 aataggtcct caaatcattg ataatacaga cgatttaatt ttaatggaat taataactgg
      541 aaaagggatt tctgattggc ttgatgatcc ttctaatttt aaccttgaca acagcaaaaa
      601 aattataact atggttaatg agatattaga gcaatgttat agattagatg ttttaaattt
      661 agatcatgga gaattaactc gcattgataa tcatgtaatg gtttctgaat ataatcaaat
      721 atcaataata gattttgaaa gctcatctac taaacgaaaa ccatccaatg ttacatcagc
      781 tagtcaagct ttacttttga gtgggggatt aatttcaaaa aaaatatgta aatttattaa
      841 aataaaagat cacaaatatt tattgaatag tttaataaat tataagaaaa gaaaaactag
      901 acaaaatttt gaagccatat tagctctagt aaatggaaaa agatagtcat cataaaaact
      961 ctaatgttta ataacataaa tagaaacaag tatgtattat tcgaaaatct cttattacag
     1021 cacctatttt attttaatca aaatgactga aaaaagcggt tctatagaat cttctaacga
     1081 tactattcca ttcccgacat taacaatttt gtatttgcca gccgatgcgt cagaggttgt
     1141 agaagaagtt agtcaaaaat attataatat gacttcagaa gactgtgttg gttattttca
     1201 tgatggagta aaacgagaat acaagaaact tacaatatgg agccaaggaa ttgacagttt
     1261 atggtttaat gctataatag ctcgaattaa agaagttgcc aaggtagagc aagtacctat
     1321 cgtatctggc ggaataatgt atactatata aaccgctaaa gatatttttt catatattac
     1381 tatatttatc tataaactaa ttatctcgtt gttcaataac ataataaaat tatatatctg
     1441 aagaaattta aaagaataag gtggagaata taacaacaac tactactgta ttcaaaatca
     1501 aacagctagt tgataatggt tcaaacgtta ctctttcttt gagtgaagat gtggaattag
     1561 aaacagtatc gcaacaacaa atgatattgg aagcagtaga tagaaccgta acagatgatg
     1621 aagtaaaaca acaaatcaag cctttattgg aagcaatttt aagatcacag ccccaaactg
     1681 taattaaaac ataccccaaa actatcattc aaattactat gccaaagaga agatatgaaa
     1741 tgatgggaaa tcctcaagtt ggagagacac tacaggtaga cataaagaaa taatattcat
     1801 catttttatt aaatgtaata ttttttatat tctatttaat ctctgctcca cctaatttca
     1861 taggtaatca ttataaaaga gaaaattatc aattttcaat ttcaataaca tcatattata
     1921 tattcattat agcctatcaa cggtgcaagg caaaaaattt gatattatca ttataggtgg
     1981 aggtatccta ggagtatcta tagggtattt tttaactgta aactcggatg caaatgtttt
     2041 aatcatagaa cgtgaaaaaa atgtttcaag tcacactagt tctagaaaca caggtaaggt
     2101 acatgcacca ttcttatacg atccaatcaa aaaaaaaatt tttgcaagaa tatctttttt
     2161 aggttataat atgtgggaaa catattctaa tagaaaaatg atgcacttta aacaagacgg
     2221 agttctagaa atagcgaagg aagaaaaaga tatcaaagtt ttaaaaaaat atgttgaatg
     2281 gggaataaaa aatggattag aagaaaagga tatactcttt ttaaatgacc aagaaacaag
     2341 aactattgaa ccaaatgtaa gatgttatgg atcaatatat tgtaaaagag atgcaagtgt
     2401 tgactacggt ctattgacga ctactttaaa aaatgattac ctcttggctg ggggaaagtt
     2461 actaaatttg catagagtca tcgggattaa acatcagaag gataattgtt caattgaaat
     2521 ggtcacaaag gatagagaga gaataattat cgattcaaaa tatcttatta atacagctgg
     2581 tggtcattca cttgatattg ctcatcaatt tgaaattgga ttagatttat ttgatttaca
     2641 ttttaggggt gaatattggc tggctcctca cgaatatgaa aaactcacaa ctcatagtat
     2701 ttattcagtt ccaaaacaac aaaattttcc atttttagac ccccactgga tcataaaagc
     2761 agatggacat tgtgaaatcg gaccaaacgc tgtccctgta tttggaccat actcatataa
     2821 tttaattgac aattttaaaa actttcttcc caaaacaaaa gaaattttat ttaagaaagg
     2881 attgtggtct cttttctaca atagagaatt cttatcatta ctaacgaatg aaattgctgg
     2941 ctccttgtca aaacgtatta tgattcaaag agtaaaacaa ttcttacctg ctttagatac
     3001 taaaaaattt aataccagag gtactgctgg aattaggtca ttacttatag acgaaaaagg
     3061 taactttatt cccgatacat tattgttaaa gaactcttta tcactacaca ttttaaatta
     3121 caattcaccg ggtgcgaccg gggctttacc tctttcagca atgatagtcc aagaattagc
     3181 caatgagaaa ctcataaata aaataacgaa aaataaccag ttatggaata tcgaagatat
     3241 ttattctaaa atcaagtttt gatgctatga ttgagtaaca attaaaaaat tttaattgat
     3301 tcagaaacta ttcaaagagt aaaaataaat tagcaatatc aatcaatata ttatctttgt
     3361 tattatttta gttaagttac tcacctcaac ttttaaatgc aaacaattgg gggttgtggt
     3421 ctagtttggt aggataccag cctcgggcgc tggggatcgt cggttcaaat ccggccaacc
     3481 ccattcacat ttcacgtagc gtctaataat aatattatat tagatgaatt aaaaagataa
     3541 atgcaaatta tgtaacattt gcctttaatc tcaaaatcaa aactaactaa gtggattaat
     3601 gttctgatgg catgtattac ttcatgaaaa gctcagacga tatactacta attataatac
     3661 ctacattcaa ttaataatac tttattcttt atgatcctgc aaggtcatat ctgctgaatc
     3721 aacctagtaa aaatgaattg caaatgtgct atagcatttg tgctacatct cctaagatta
     3781 cctactacta ccaacctctt taattatata ttggtatgac catcaatcac aaatcatttg
     3841 taaattataa ataataatgt actaatggtt gtatcatcat agataaaaag ttacaaacgt
     3901 gaatttcatt tcttagacat ttcaatatca tacccaccgg tagtaaacct tcttttgttt
     3961 ccaaatggtt tgaatggtct tcctgctcca tgataaaatt tggaaaagaa ttcaacataa
     4021 atcaatcttg ctccttgtat tccaggttca aacactccaa tgataatatt aaaagtgtga
     4081 ccaatcaaaa agatcataat tcctaatata ctgagagcta ttgaattatc taaagatttg
     4141 aggaagatga aatcaataac atgagcaaga ataactgatg caagcaatat tccgacaatc
     4201 ctggtataag aaagtatgtg actgattatt gatggcaatt ccataagtgc tctgagtccc
     4261 tcaccataaa acatcattcc tattcctcca aacagtaata caaaataacc aactccttga
     4321 acattctgca agggatttat attttcatga tttatcatcg ccaagcctac cagagcaatt
     4381 ccccaaccaa ataatagcca tccaactttg ctaataatat gttttatctg atgctccctc
     4441 atactgttaa gtatccctag tacaagccca aatgaaacca tacccaatcc aacatatcca
     4501 ctaaatagca aaagtttacg cagaccaaaa gttccaatag gattcaaaaa tgcaccatca
     4561 gatggcaagt ttaacccaaa ctggttattc aaatatgaga acagataccc atttaggtgg
     4621 aatccgaaat aaaggtcaaa gcaaaatccc aatacaattg caataataca acctggaatc
     4681 atagctttag ctagcttaac catctgtgaa gggcgtagaa tggttttggc gaaatttctt
     4741 aaaaattttg gcataatggt caggttactt tttttatcct gcactcttct gattacccat
     4801 aaagaaacca gaagtatcac taatccatat cctacatcac caatcattaa accgtaaaat
     4861 ataggaaaaa ttaaaccaaa aatcaaagta gggtcaaatt cgttaccaga aggtaaagag
     4921 taaaatctta caaaggattc aaaaactttg
//
LOCUS       DASVEV010000121         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1402039, whole genome shotgun sequence.
ACCESSION   DASVEV010000121 DASVEV010000000
VERSION     DASVEV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402039"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF222_05445"
     CDS             complement(<1..555)
                     /locus_tag="VF222_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX7141904.1"
                     /translation="MATSSYSASFGRLQSLSINFISQNILQNLLHAKDVAEMTRILEP
                     TWYGPEIERAASLYSLPELLEVALNRHIVEINKMALESTPFSGRDAIRAYLLKWDIHN
                     IELILSSKIIGRAITETEPFLVSSRNFPAGISAGNISHDEMKIILSQAGVEGVVNHLV
                     KYRYGTILMQHLDTYQKTGDLGPMM"
     gene            complement(558..1121)
                     /locus_tag="VF222_05450"
     CDS             complement(558..1121)
                     /locus_tag="VF222_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HEX7141905.1"
                     /translation="MSLDTFIQEIEERKTRKINLLDITLTEKKTRIKHTMESTLKEMQ
                     QHYADEAKAKSQKEAAKIVEAARLKAKKILFDAINENMDSTFNTIREELKSYSQKPDY
                     KITIKKMIKYAKMKLNSQDIIIHYRIDDNIILNGMKIITGSPIQTIGGILAENKNGTM
                     EIDLTFEELLRTHEDDIQNFLLERLMK"
     gene            complement(1143..1487)
                     /locus_tag="VF222_05455"
     CDS             complement(1143..1487)
                     /locus_tag="VF222_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141906.1"
                     /translation="MIRYNGSKLSIIAIIAGLALVGFTLSSNQVFAAEPSTSSSEGLT
                     KPLLAIAAAIAIAGGLIGTGNAQQGIGAAGMGIIAEKPEKFGQVLFFFVIPETLWIIG
                     FVLGIILLLGIL"
     gene            2335..2475
                     /locus_tag="VF222_05460"
     CDS             2335..2475
                     /locus_tag="VF222_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141907.1"
                     /translation="MKVNKKLTRNGPDVYSWRDMVKGSVIVIGAFSIFYTFIMYVTGG
                     LF"
BASE COUNT          952 a          432 c          412 g         1127 t
ORIGIN      
        1 catcatcggt ccaagatctc ctgttttttg ataagtatca agatgctgca tcaatatggt
       61 tccatatctg tattttacaa gatgatttac aacaccttct actccagcct gtgataaaat
      121 aattttcatt tcatcatgag agatatttcc tgctgaaatc ccagcaggaa aatttcgact
      181 tgagacaagg aaaggctcag tttcagtaat agcacgacct ataatctttg aggataaaat
      241 taattcaatg ttgtgaatat cccattttaa tagataagct ctgattgcat ctctaccgct
      301 aaatggtgtg gattcaagag ccattttgtt aatttcaaca atatgtctgt tgagggcaac
      361 ttcaagaagc tcaggaagtg aataaagaga tgcagctctt tcgatttcgg gtccatacca
      421 agttggttcc agaatccgag tcatttcagc aacatctttt gcatgcagta aattttgaag
      481 gatattttga gaaataaaat ttattgatag ggattgtaat cggccaaacg aagctgaata
      541 cgatgacgta gccatactta cttcatcaat ctctctaaaa gaaaattctg aatgtcatct
      601 tcatgggttc ttaagagctc ctcaaaagtc agatcaatct ccatagtccc atttttgttt
      661 tctgctaaaa ttcctccaat agtttgaatt ggagaacctg taattatttt cattccattt
      721 aaaatgatat tgtcatcaat acgatagtgg attatgatat cctgactatt caatttcatt
      781 tttgcgtact ttatcatttt tttaatggta attttataat ctggtttttg actatagctt
      841 ttcaattcct ctcttattgt gttaaacgta gaatccatat tttcattaat tgcgtcaaat
      901 aagatttttt ttgcctttaa tcttgctgcc tcaactattt tggctgcttc cttttgtgat
      961 tttgctttag cctcatctgc ataatgttgt tgcatttcct tgagggtact ttccattgta
     1021 tgcttaatcc ttgttttttt ttctgtcaaa gtaatatcaa ggaggttaat tttccttgtt
     1081 tttctttcct cgatctcttg gatgaatgta tccaagctca tgatttattc atactcctac
     1141 tactataata taccaagtaa tagaatgatt cctagaacaa aaccgatgat ccaaagagtt
     1201 tcagggatga caaagaagaa aagtacctgt ccaaactttt caggtttttc tgctattatt
     1261 cccattcctg cagcaccaat accttgctgt gcattgccag tacctattag acctcctgct
     1321 attgcgattg ctgcagctat tgccaacaat ggctttgtta atccctcgct tgaagaggtt
     1381 gagggttcag cagcgaatac ttgattagaa gatagtgtaa atccgacaag cgccaatcct
     1441 gcaattattg caataataga caattttgaa ccattatatc gtatcattat tagctatggt
     1501 taattataat tagacattta taagagtttt cttaatcttg taactctttt tattatttat
     1561 ttagaatact aaagtcttgc catcttgtaa gtgatttcat aaattttaca gcaaacgtgt
     1621 atataatata aattataaaa tatccttgac gttttaataa gtataactaa tcaatacaat
     1681 atgtatctga gaagtagttt tatgaaataa tttttaatag ttagttacgc atcgctttac
     1741 taaagggtta gattgcaact aattcaaact gaaggtagat aataactttt attcatttat
     1801 aatttgtatc ttggattttt tgatttcatt aaataaatct attaagaatc tcattttaaa
     1861 tatgaaaaag tatttgttta ttgatgcatc ttttctatat gtctcgtaac aaataatatt
     1921 ctaacaacat tggtctacaa tatatctcta tattttatta aatattaaaa tgcagtatta
     1981 tgatcaggtt tgacttaaat attttatttt gagataatat caaataatat tataaatagt
     2041 tgtgttgact attcgagcaa gtgaaaattc aaattggacc tttgaataat aagtcaagaa
     2101 gcttaatgaa agatttgatc ttaactttgt aactataata caaagcagat actcttacaa
     2161 aaaatgattt tatattataa catctctaaa tcttaaataa ttaaatatta ttataaatga
     2221 attgttttct aaattttaca aattaatccg atataaacca aaataaatta gtttaattga
     2281 ttatttttaa atatttagat atattttaaa tataggtgta taatatatat aattatgaag
     2341 gtaaacaaga aattaactag aaatggacca gatgtctaca gctggcgaga catggtaaaa
     2401 ggttcggtta ttgtgattgg agcatttagt attttctata cattcataat gtatgttacc
     2461 ggcggattat tttaataaat tacttcttaa ttactaattt ttttatcgga aaagaattgt
     2521 aaagtaccat tttttaatga aggtctttcc gttctatttt gattttcttt aaaatctaac
     2581 ttttttggat tttacatttt gaatacatta agagattact attcgattaa attggattgt
     2641 agttcctcca gattataatt tagaaaattt atttaatgga tgttacttta cgctctaaat
     2701 ttcttaaatc tctttacaaa tttatcctta gagcatctaa aaccatttta aagagcattt
     2761 gatattataa aagtgtaaat tctaaactac gtattctatt attttaaggt tacagatcat
     2821 ttattttaaa taattttctt ttttattcag atggtcaact ataataacca agtcctagaa
     2881 atatatgcat atgtatatgt atacgttttc atttatagtt aca
//
LOCUS       DASVEV010000122        13357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_431458, whole genome shotgun sequence.
ACCESSION   DASVEV010000122 DASVEV010000000
VERSION     DASVEV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431458"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF222_05465"
     CDS             <1..500
                     /locus_tag="VF222_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141908.1"
                     /translation="QKYNMKVACVVDMPVEIGISKILGMEIIEAGSVNDYVTKAKVTA
                     KKLQNYDMIYVHIKGPDEFGHDGDALGKKQNIEKIDSDFFGTLLRELEISNAVIVVSG
                     DHSTPCIKKSHTDDPVPLLISTKILKNTKIQRFTESYAKTGVIGMISGSDVLKKTLEI
                     MNAKK"
     gene            complement(524..931)
                     /locus_tag="VF222_05470"
     CDS             complement(524..931)
                     /locus_tag="VF222_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7141909.1"
                     /translation="MLLPSEIEAKSLIPAIRAMLAKKLTIEYKMKEEFVAKVLGVTQA
                     AISNYIRGTRGDTDLINKLSSILEIMRKIDDIAQDLVANRVYTPSTMAKFVELCNFMR
                     YSFIICDVHHKMEANIDEQVCELCKDNLIGSKF"
     gene            1109..2197
                     /locus_tag="VF222_05475"
     CDS             1109..2197
                     /locus_tag="VF222_05475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7141910.1"
                     /translation="MLLKTKFGFTQGLNVARLGLDESQVITACQMADRLDFDSIWAMD
                     HTNVPQWKNAVVNDAWLLLAAIGALTTNVELGTCVTDAIRRHPSTIALSTVTLDRLTN
                     GRGMLGIGAGEAQNVVDFGIEFNKPVTKLKEQLEVIEKLFHSDPDNRVDYSGQYYKLT
                     NACLQAKSIRKPRPPVYIAAGAPKTLELCARYGDGWIPIGYTPELFKTHANVIHEHAK
                     NFGRDLSHFQFANDVDVYFTDDGEEAWNKMKNAVKVSLYKPELLKVHNLAQDSEFDFR
                     KYFTEYTMDKPELMEQMKKAALKIPDSVARTAIGVGQADEVIEMLERFIKAGTNHFII
                     RFWGDGYFKNIELFGKKVIPYFRAQEQK"
     gene            complement(2252..3316)
                     /locus_tag="VF222_05480"
     CDS             complement(2252..3316)
                     /locus_tag="VF222_05480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7141911.1"
                     /translation="MHSRIRSMDHGLLLENNETRILLDPSKVESNDFVFISHAHSDHV
                     YRKPRNKSKIKTITSVETEAIANARGYEITNAIYEEKDLELIDSGHILGSKGLLAFNE
                     IFYTGDISIRKRAFLKLPKKIPQVDTLIIESTFGRPEYIFPDHREIIHQANKLISDAY
                     DKGHPVILMGYPLGKAQLLTNFFGHWDPFFSYDSIEVINTVYRNMGITLRNTTSFTEA
                     QKNEKIDTKYPWVMLAPLMNGNSKFLKYMKEKYNTITIGFSGWACSQRYKYMMGIDHA
                     LPLSDHCDFNELIQVVKKCNPKKIITIHGFAVEFAKILNNMGYNAMPIFQKTNKNKKI
                     AKEPNPRRKKDIQILDYFIK"
     gene            3477..4091
                     /gene="purN"
                     /locus_tag="VF222_05485"
     CDS             3477..4091
                     /gene="purN"
                     /locus_tag="VF222_05485"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7141912.1"
                     /translation="MAILISGRGSNMNAILSSHAQSELKNIQPSVVISNKKEAPGLKI
                     AKEKFGIATEVILSHGKKGWEYDQEIDSVLKNYGVYPSNGIICLAGYMRILSTEFVSK
                     YKLRILNVHPSLLPSFKGLHAQKQALDYGVKISGCTVHLIDDDVDTGPIIIQECVPIY
                     DSDTEEDLSNRILQLEHKLYPLAINLISDGKLKLDGRKVHLLKK"
     gene            4124..4438
                     /locus_tag="VF222_05490"
     CDS             4124..4438
                     /locus_tag="VF222_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141913.1"
                     /translation="MYNSFVPNDFLINHTLKLHYFVPSERKLWTIVGKNNEYWLDPEL
                     DYCSCKHYYYKTLSGKEKCQHLKIFNELLKNNNYDKIKFSDDEYSNFLTILIKDILKI
                     LK"
     gene            4535..5803
                     /gene="purD"
                     /locus_tag="VF222_05495"
     CDS             4535..5803
                     /gene="purD"
                     /locus_tag="VF222_05495"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7141914.1"
                     /translation="MKTNILIVGSGGREHALGWKLSHSPSVAMVYYCPGNGGTINNLS
                     YSITDFSKIKSFAIKNNCIVIVGPEEPLTKGIVNELSDSIRIFGPTMEAAKLEASKSF
                     AKKFMKQHNISTAKFNTFSDPEKAKDFVYSTDKSLVIKADGLAAGKGVVVCDNKEDAL
                     NAIDSIMIKKNFGNSGDVIVIEERLFGEEISFIGISDGDTIIPMASAQDHKRIYDNDN
                     GPNTGGMGSFSPSNIISEDLFNDIINRIMKKTVHSMKRNSNPFVGFLYAGLMIEKDTQ
                     KPYVLEYNVRMGDPECQPIMMRMKSDLFDYIKCSISGTLHTLPPINWYENPAVCVVMA
                     EKGYPSNYETGNVIHGLNSSFGHNIKIFHAGTIRNNTNDILTYGGRVLGVTAIGIDFN
                     NARSDAYKAVKKIHWGKDSEYYRKDIGLKA"
     gene            complement(5811..6389)
                     /locus_tag="VF222_05500"
     CDS             complement(5811..6389)
                     /locus_tag="VF222_05500"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7141915.1"
                     /translation="MKKNFLTKYQIRQEILRIRNSLSETEIVSNSNIISNKVISTKEF
                     KKAQSIGTYYPIRSEVKTFEIIKYSLATKKTIGLPRIIDSNRIAFFKIMENSFEDIKL
                     TKGKYGILENLISDSILEEMDLLIIPGIAFDLQGNRIGYGKGYYDRFLSTRKVSYIMG
                     LAFENQIIKKIPTTENDIPVDVLVTENRIIFV"
     gene            complement(6395..7243)
                     /locus_tag="VF222_05505"
     CDS             complement(6395..7243)
                     /locus_tag="VF222_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX7141916.1"
                     /translation="MIINGLEVSSMIKEELIIEIQSLKKRSVFPCLSTILVGDNPASA
                     IYVRNKHRACSEVGIKTKDNNIHSSIKEKNLIELIKSLNEDQSVHGILLQLPLPDHIN
                     QFNVINAIEPQKDIDGLTYYNAGLLLNKKPSFIPCTPLGVMELLKYYSVELKGKDAII
                     VNRSILVGKPLALLLLDKDATVMICHSNTKNLNEKLMNSDIIITAVGNRKLFKLTADF
                     VRNDAVVIDIGISRANGKVIGDVDYENVSKKASLITPVPGGVGPMTIAMLLKNTVLAA
                     TRLMSR"
     gene            7399..8178
                     /locus_tag="VF222_05510"
     CDS             7399..8178
                     /locus_tag="VF222_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141917.1"
                     /translation="MTKATPINSHAALLLEYVEKGGEKKKFLVISDLHLGSETNLIKK
                     GINIDSHGIARDILKEIDELKALHSLNGIVILGDLKSSITQITKNEWETIPIFLKELS
                     LDKDIFLIPGNHDVNINLLAPESVIVCNSSGLIIDDILLIHGHTIPEKTKFNINKIIM
                     GHIHPIFINSNSVVHGKRLWIYLKVKKESIFGLKTKGTIEIIIVPTFNKYFYSLSYHK
                     ESSSISPLMRQLIKKDAILSGLLFTLEGSLIGETNEIKDLF"
     gene            complement(8188..11361)
                     /gene="carB"
                     /locus_tag="VF222_05515"
                     /pseudo
     CDS             complement(8188..11361)
                     /gene="carB"
                     /locus_tag="VF222_05515"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
     gene            complement(11500..12666)
                     /gene="carA"
                     /locus_tag="VF222_05520"
     CDS             complement(11500..12666)
                     /gene="carA"
                     /locus_tag="VF222_05520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7141918.1"
                     /translation="MPILYPKYTGFSAKLLLENGKSFSGIGFGYPSQTAGEIVFNTGM
                     VGYIESLTDPSYSGQILSMTYPLVGNYGVPDKNIRDEFGIPKYFESEKIQPKGLVIHS
                     LSNVASHWSCVKTLDEWLYEEKIPGIYGIDTRELTKIIRQKGVMMGVLTVSDKPIDKI
                     DFRKIINLENYNTKNFMSSVSSNDVKIYGETNNEVLVLLDTGAKYSIIRHLLRIGFKV
                     VQVPWDSTYEKILSYNPVGVIISNGPGDPKMCKQTINTAKKLIDTSIPTLGICLGNQI
                     LALSAGADTYKLKYGHRGQNKSCINKENSLSYITSQNHGYGIDPTSLTSTDFQPWFYN
                     PDDKTIEGIIHNSKPIIAVQFHPEASPGPNDCNYVFDKFKEIVNLNKRAKLPNK"
     gene            complement(12741..>13357)
                     /locus_tag="VF222_05525"
     CDS             complement(12741..>13357)
                     /locus_tag="VF222_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141919.1"
                     /translation="EEDDDESNRNTSQEEDDDESNRNTSQEEDDDESNRNTSQEKTRE
                     VFLKKSNLLSTNQLSGDKTIGNSIDNENNKLISQFSELNSSFRSDLQIPSVEANSKLE
                     DRKPIANAGLDQVIMSDQNIVLDASASFDPEGKMSSYLWKPIENSEDNPNTSNSMIYS
                     FPIPEDMEGNTLEFKLMVMDENGQEDTDTVKFLINEEDKEESEP"
BASE COUNT         4374 a         2169 c         2017 g         4797 t
ORIGIN      
        1 atcaaaaata taatatgaaa gttgcttgcg tagtggacat gcctgtagag attggtatct
       61 ctaaaatttt aggaatggaa attattgaag caggaagtgt aaatgactat gtaaccaaag
      121 caaaagttac tgctaagaaa ttacaaaatt acgatatgat ttatgtacat attaaaggtc
      181 cagatgaatt tggacatgac ggggatgcct tgggaaaaaa acaaaacatt gaaaaaatag
      241 atagtgattt ttttggaact cttttgaggg aattagaaat ttcaaatgct gtcatagttg
      301 tttccggaga tcattcaact ccctgtataa aaaaaagtca tacagatgat cctgttccgc
      361 ttctcatatc aactaaaatt ttaaaaaata ctaaaattca gagatttaca gaatcatatg
      421 ctaagactgg agtgatcgga atgataagcg gatcagatgt tctaaagaag actttagaaa
      481 ttatgaatgc aaaaaaataa ccttggaaaa attctttttt tatttaaaat tttgagccaa
      541 taaggttatc tttacataat tcgcatactt gttcatcaat attagcctcc attttatgat
      601 gaacatcaca tataataaat gaatacctca taaaattgca taactcgacg aatttagcca
      661 ttgtgctagg agtatataca cgatttgcaa ctaaatcttg agcaatatcg tcgattttgc
      721 gcatgatctc caaaatagat gaaagtttat taataaggtc tgtatcacct ctggttcctc
      781 taatataatt actaatagca gcctgggtaa ccccaaggac tttagccacg aattcttcct
      841 tcattttata ttcaatagtt aatttttttg ccaacatggc tcgtatagca ggaattaagg
      901 atttagcctc tatttcagaa gggagtaaca tctaattcta atagaaagaa aaattatata
      961 agttttcttt aattgttatt ggccaaaaat gaaattatat gctattttct gaaaatggtt
     1021 aaatataact ctaatttttt ttaccttata tggttataaa taacctgtta gtatccttta
     1081 acatatttgt caaaatgtgg aggttaaaat gttattgaaa acaaaatttg ggtttacaca
     1141 aggtctgaat gttgctagat taggattgga tgagagtcag gtaattactg cgtgccagat
     1201 ggctgataga ctagattttg actccatctg ggcaatggat catacaaatg ttcctcaatg
     1261 gaaaaatgcc gttgtgaatg atgcttggtt attgcttgcc gcaataggag ctctcacaac
     1321 gaatgtcgaa ttaggtacat gtgttacaga tgctataaga cggcatccat caacaattgc
     1381 tctttctaca gtgacattgg atagattaac gaatggaagg ggtatgttgg gaataggtgc
     1441 aggagaagcg caaaatgtgg tagattttgg aatagaattc aataaaccag tgactaaatt
     1501 aaaggaacaa cttgaagtaa tcgaaaaact ttttcactct gatccagata atagagtaga
     1561 ttatagtgga caatattata aattaacgaa tgcatgttta caggccaaaa gcatacgaaa
     1621 accacgacca ccagtgtata tcgctgcagg tgcgccaaaa acactagaat tatgtgctag
     1681 atatggtgat ggttggattc ccataggata tactcctgaa ctattcaaga ctcatgctaa
     1741 tgtaattcat gaacatgcca aaaattttgg tagagatctt tcgcattttc aatttgcaaa
     1801 tgatgtggat gtgtatttta ctgatgatgg tgaagaggcc tggaacaaga tgaaaaacgc
     1861 agttaaggtc agtctttata agccggaatt attaaaagtt cataacttag ctcaggactc
     1921 tgaatttgat tttagaaaat attttacaga atatacaatg gataaaccgg aactaatgga
     1981 acagatgaaa aaagcagcat taaaaattcc cgatagtgtt gccaggactg ctataggagt
     2041 aggacaggca gatgaagtaa tagaaatgct ggaacgtttt ataaaagctg gaactaatca
     2101 ttttataata agattctggg gagatggata tttcaaaaat atagaattat ttggaaagaa
     2161 ggttattccg tattttagag cacaggaaca aaaataacca agttattatt tttctttgta
     2221 ttattgttta aatataataa ctagaaggaa cctatttaat aaaatagtct aatatttgaa
     2281 tgtctttttt tcttctcgga tttggttctt tagctatttt tttattctta tttgtttttt
     2341 gaaatattgg catggcatta tatcccatat tgttaagtat ctttgcaaat tctactgcaa
     2401 atccatggat tgttattatt ttttttggat tgcatttttt tactacctga attaattcgt
     2461 taaaatcaca gtgatcactc aaaggaaggg catgatctat tcccatcatg tatttatatc
     2521 gctgactaca agcccagcca ctaaacccta tggttatggt attgtatttt tctttcatat
     2581 attttaaaaa ttttgaattt ccattcatta atggtgctaa cattacccaa gggtatttgg
     2641 tatctatttt ttcatttttt tgtgcttctg taaagcttgt tgtgtttctt aatgttattc
     2701 ccatatttcg atataccgtg tttattactt ctatgctatc gtaagaaaaa aaagggtccc
     2761 aatgaccaaa aaaatttgtt aacaattgag cttttcctaa cggatatccc atgagaataa
     2821 ctggatgacc tttatcataa gcatccgaaa taagcttatt tgcttgatga attatttctc
     2881 tatgatccgg aaaaatgtat tcaggccttc caaatgtaga ttctattatc agagtatcga
     2941 cttgaggtat ttttttaggt aacttaagga atgctcgttt tcttattgat atatccccgg
     3001 tataaaatat ttcgttgaaa gctagtaatc ctttcgatcc taatatgtga ccggagtcta
     3061 tcaattctaa atctttttct tcatatattg catttgtgat ttcataacct cttgcatttg
     3121 ctattgcctc agtctctact gaagtaatag tcttaatttt tgatttattt ctaggtttac
     3181 gatatacatg atcagaatga gcatgagaaa taaatacaaa atcattactc tcaaccttgg
     3241 aaggatctaa caaaatcctt gtttcattat tctctagtaa caatccatga tccattgatc
     3301 ttattcgaga gtgcatgaca agctattctg taccatatat tttaattaac tgtttagatc
     3361 tttgtgatgt atcatcagtg attaccttag atgctattat ctatatgagt ccataaacac
     3421 aaacttcaaa agaagtgtac attaaagatc atttagtaat aaatattatg cttaaaatgg
     3481 ctatattgat ttctggaaga gggagtaata tgaacgccat cttatcatct catgcacaat
     3541 cagaattaaa aaatatacaa cctagcgtgg ttatatcaaa taaaaaagag gctcctggat
     3601 taaaaattgc aaaggagaaa ttcggtatag caacagaggt tattttaagt catggaaaaa
     3661 aaggttggga atatgatcaa gaaatagatt ctgtattaaa gaattatggt gtgtatcctt
     3721 caaatggtat tatctgtctt gccggctata tgcgaatatt gagtacagag tttgttagca
     3781 aatacaaact tagaattctt aatgtacatc catcactgtt accctcattt aagggtctac
     3841 atgcccaaaa acaggcgctt gattatggag taaaaatttc tggctgtaca gttcacttga
     3901 tagatgatga tgttgatact ggaccaatta ttattcaaga gtgtgttcct atttacgata
     3961 gtgacacaga agaagattta tctaatcgaa tattacaact agaacataag ttatatcctc
     4021 ttgcaataaa ccttatttct gatggtaaat taaaattaga tggacgcaaa gtacatcttt
     4081 taaagaaata atttaatata tccaaatggt tcgaatatta ttaatgtata attcttttgt
     4141 tcccaatgat tttttaatta accatacact taagcttcat tattttgttc ctagcgaaag
     4201 aaaattatgg actattgttg gaaaaaataa tgaatattgg ttagatccag aattagatta
     4261 ttgctcatgt aaacattatt actacaaaac gctttctgga aaagaaaaat gtcaacattt
     4321 aaagattttt aatgaattgc taaagaataa taattatgat aaaataaaat tttcagatga
     4381 tgaatattct aattttctta caatactcat aaaagatatt cttaaaattt taaaatagaa
     4441 gaatagatgg ttatattgaa aaaactttag gtatattatt attttatttt aaaaaattat
     4501 atagaaatat atcacattta atatcactta cgaattgaaa acaaatatac ttattgtagg
     4561 gtctggagga agagaacatg cattaggatg gaaattatcg cacagcccat ctgtagctat
     4621 ggtatattat tgtcctggaa atggagggac aataaataat ctttcttatt ccataaccga
     4681 tttttcaaag ataaaatcat ttgcaataaa aaataattgt attgtgatag tgggtccaga
     4741 agaaccatta acaaagggaa ttgttaatga attgtcagat agtataagga tattcggacc
     4801 caccatggaa gcagccaaat tagaagccag taagtctttt gcaaaaaaat tcatgaaaca
     4861 acataatata tctacagcta agtttaatac tttttcagat cctgaaaaag ccaaagattt
     4921 tgtatacagt acagataagt cattagtaat aaaagcagat gggcttgctg cgggaaaagg
     4981 agttgttgta tgcgataata aggaagatgc tctgaatgct atagattcta taatgataaa
     5041 gaagaatttt ggaaattctg gtgatgttat cgtaatcgag gaacgtctat ttggtgaaga
     5101 gatttcattt atagggatat cagatggtga tactattatc cctatggctt cagcccaaga
     5161 tcataaacga atttatgata atgataatgg cccgaacacg gggggcatgg gaagtttttc
     5221 cccgtccaac attatatcag aagatctttt caatgatata ataaatagaa ttatgaaaaa
     5281 aactgtacat tctatgaaaa gaaatagtaa tccatttgta ggttttctat atgctggttt
     5341 gatgattgaa aaagatactc aaaaaccata tgttttagaa tataatgtac gaatgggtga
     5401 tccagaatgt caaccaataa tgatgagaat gaaatctgat ttatttgatt atattaaatg
     5461 ttcaatctcg ggaactctgc acactttacc tcctattaat tggtatgaaa acccagcagt
     5521 ttgtgtagtc atggcagaaa aaggatatcc atcaaattat gaaacaggaa acgttattca
     5581 tggattgaat tcttcttttg ggcacaatat taaaattttt catgctggaa caatacgcaa
     5641 taatactaat gacatcctta cctatggagg aagagtctta ggagttacag cgataggaat
     5701 agatttcaat aatgctagat cagacgcata taaagcagta aaaaaaatac actgggggaa
     5761 agatagcgaa tattaccgca aagatattgg tttaaaggct tgaatagaat tcagacaaaa
     5821 ataattctgt tctccgtgac taatacatca acgggaatat cattttcagt agtaggtatt
     5881 ttctttatta tctgattttc aaatgccaac cccataatgt agcttacttt tcttgtagaa
     5941 agaaatctat cataatatcc ttttccataa cctattctat ttccttgcaa atcaaatgca
     6001 attccaggaa taatcagtaa atccatttct tctaaaattg agtcagatat caaattctct
     6061 aatattccat attttccttt tgttaatttt atatcttcaa aagaattctc cataatttta
     6121 aagaatgcaa ttctgtttga gtcaataatt cttggtaaac caatagtttt ttttgtggct
     6181 aatgaatact taattatttc aaatgttttc acttcagatc taattggata gtatgttcca
     6241 attgattgag cttttttaaa ttcctttgta gaaatgacct tattactaat aatattacta
     6301 ttgcttacga tttcagtttc agaaagagag tttcttatcc ttaaaatttc ttgacgtatc
     6361 tgatattttg ttaagaaatt tttcttcatt atttttaccg actcattaat ctagtggctg
     6421 caagaactgt attttttaaa agcatagcta tcgtcatcgg tcctacacct ccaggaactg
     6481 gagttatcaa agaagctttt ttagaaacgt tttcataatc tacatctcca attactttcc
     6541 catttgcacg tgatattcca atatcaatta ctacagcatc atttctcaca aaatcagctg
     6601 tgagtttaaa taatttcctg ttgcctactg cagtaatgat tatgtcggaa ttcattagtt
     6661 tttcattaag gttttttgta ttagaatgac atatcataac cgttgcatct ttatcgagta
     6721 aaagtaatgc tagaggtttt cctaccagaa tacttcgatt tactataatt gcatcttttc
     6781 cctttaattc aacactatag tatttgagta attccattac tcctaatgga gtacaaggaa
     6841 taaaggatgg ttttttgttt agtaacaagc ctgcattata atacgttagt ccatctatgt
     6901 ctttttgagg ttcaattgca ttaataacat taaattgatt aatatgatct gggagaggta
     6961 gttgtaataa gattccgtga accgattgat cttcattcaa tgactttatt aactcaataa
     7021 gattcttttc tttgattgat gaatgtatat tattatcttt tgttttaata cccacctcag
     7081 agcatgctct atgtttattt cttacatata ttgctgaagc aggattatct cctactaaaa
     7141 tagtagacaa gcagggaaaa acactccttt tctttaaact ttgtatttct ataattaatt
     7201 cttcttttat cattgatgaa acctcaagcc cgttgattat caacttaagt tatacaaaat
     7261 taacttattt ataaaaccta tcgagttaag aaactacaat atttgactca gaattattta
     7321 tttgttcttt tcaagttaaa agtaaataca agaattataa tgtgaaaatg ataaatagat
     7381 tataacagta atattgatgt gactaaagcc actcccataa attctcatgc agcattatta
     7441 ttagaatatg tagagaaagg cggtgagaaa aaaaaatttt tagtaatatc tgatcttcac
     7501 ttaggttcag aaacaaacct aataaaaaaa ggtataaata ttgattctca tgggatcgca
     7561 agagatattt taaaagaaat tgatgaatta aaagctttac attcattaaa tggcattgta
     7621 attttgggcg atctaaaaag ctctatcaca caaattacaa aaaatgaatg ggaaacaata
     7681 cctatttttc tcaaagagtt atctttagat aaggatatat ttttaattcc aggaaaccat
     7741 gatgtaaata taaatctact tgcacctgag tcagttatag tttgtaattc cagcggatta
     7801 attattgatg atatattgtt aattcatgga catacaattc ctgaaaaaac aaaatttaat
     7861 ataaacaaaa ttatcatggg tcatatacat cctattttta ttaattccaa tagcgttgtt
     7921 catggaaaaa gattatggat atatctaaag gtaaagaaag aatctatttt tggattgaaa
     7981 acaaaaggta caatagaaat aataattgtt cctactttta acaaatattt ttattcttta
     8041 agttatcata aagagtctag ctctatatca ccactaatga ggcaattaat aaaaaaagat
     8101 gccatactct caggattatt gttcacctta gaaggatcgc ttataggtga gactaatgaa
     8161 ataaaagatt tattttaatt aaattactta attatctatc tttaagtatt ttttaagagg
     8221 ttcgatagta ggcgaatttg tttgtagcca ctggagagtt tttataaatg acgaggctgc
     8281 ctcaaatgta gtcatcactg gaattcccat ttctacagct ttccttctaa tataatattc
     8341 atcatttaac atattcaggt atttttccat tgtagaagta gacggaatgt taatgataaa
     8401 atctatctta tgttgatata aaaaatcaat aatatttggc tttctttcag gttcattgat
     8461 tttataaact ctttgaatat ctttaattcc tttcatttct aagaaatcag cagtatgttc
     8521 tgtagccatg attttataat ccattgaggt cataagggat ataactggca gtaatttttc
     8581 cttatttttc tgtcctccta tggtaattaa tgaagttcct tctattggta aagaataacc
     8641 cgctgctatg tacgctttac ttaatgcatc ataaaaactt cttccaaaac atgctacttc
     8701 ccccgtagat tgcatttcaa ccccaagcac aatgtccgct ccttctaatt gcatgaatga
     8761 aaattgaggg actttaatag caaatccact tgttttcagc cataattctt catgaatatt
     8821 aggtaatggc ttattcaaca tcgctcttgc ggctaagtca ataagattta tccccacata
     8881 ttttgaaaca aatggcatcg atcttgatgc tcttatattc gcttcaataa ccaaaacttc
     8941 ttcattttta actagaaatt gaatattgaa aggacctgaa acattcaacg ctcttacgat
     9001 tttattagaa taatctgtta ttatatccat tatatttctt gttaatctcc atggaggtat
     9061 acacatcatt gcatctcccg agtgaatacc tgcattatct atatgttcta ttatagaacc
     9121 aacaattatc ttatcttttg aaccaacacc atcgacttca acttcagaag catcttgata
     9181 aaatttactt attactattg gatgatctgg acttacgtca gttgcattct ctataaattt
     9241 ttctaactgc ttatcgtccc agaccacttt catagctgca ccactaagaa catatgaagg
     9301 cctaaccaat acagggtatt gaactgattc cgcaaaagtc tttgcctcag ctagatttgt
     9361 aaattttttc catggaggtt gtctaatctt taacgaatct aaaagtgttc caaacttttc
     9421 tctatcttcg gctctatcga catcataact cgtagttcct attatctttg caccactctt
     9481 tgccaaactc tgtgttaaat tatttgcagt ttgacctcct acacatgtta ttataccatc
     9541 tgatttctct ttttcataaa tatccatgac tctttccaaa gtaatttctt caaagtataa
     9601 tcgatcagaa acatcaaaat ctgttgaaac ggtttcagga ttacaattga tgattgagac
     9661 ttcattaata ccattgtttt ttaaacccat aaccatattc accgtgcccc aatcaaattc
     9721 aacactactt ccaattctat acggacctgc tcccaaaact attgcatttt tacctttctt
     9781 tttaattttg acatcgtcat aatctccacc atatgtaaga tatagataat tggtctgggc
     9841 aggccattct gcagcgaggg tatcaatttg ttttattaca ggttcaattc caaaactttt
     9901 cctaagattt ctcatttgtt gttcatctaa attgagatag taagctaatt gttgatcgga
     9961 aaagcctaat cgttttgctt ctcgaataag attttcttga aagttattag gtgatttttt
    10021 aaattttttt tccatttcta tgatattttt gattttgttt aaaaaccatg gatcgatact
    10081 agataattca tttatccttt caattgtcat cccaaaataa attgctttaa ctaaatctga
    10141 taaaatattg tcattaggat tcaaaagcga atattctata ttttctaatt cttgtatttt
    10201 tttttcttct aatctatgat tgccaacaat tccaattttc ccggtatcac acattctaat
    10261 tgctttttgg attgcttcct caaatgttct tcctatagcc attacttcac caacagattt
    10321 cattgcagtt ccaagtgtag tattcacttt atcaaattta ttgaaatccc atcgtggcat
    10381 tttcactaca atataatcta aagaaggttc gaacaaagct gtagtttttt tagtaacttt
    10441 gttaattagt tcatccaaag tataaccaag tcccaatttg gcagccatgt atgctaaagg
    10501 ataaccagtc gctttgctgg ctaaagcaga tgatctcgat agtctggcgt ttatttctat
    10561 tgcgcaataa tcttcggaag caggatctag accaaactga atattacatt cacctattat
    10621 accacattca cttgtagccc gcatagcggc tgaacgtagc atatggtatt cataattatt
    10681 aagtgtctgc gacggagcta ctactatatt atcgccagta tgcactttca tagctaatat
    10741 attttccata ttacaaacga ttatgttatt ccctttgtga tctctcatta cttcatattc
    10801 tatttgtttc caatgaccaa catatttttc aattagaact tgtttactca tgcttaaaga
    10861 aatacctcta tgtacaattt catgaagctc atattcattg tgcgctactc ctcctccttt
    10921 ccctcccaag gtatatgcca ctcttacaat aactggataa ccaatatttt ttgcaacttt
    10981 tattgcttct tctacagaat aggacggtga gctaggtaat acagttacat tacttttttg
    11041 cattgcttct ttaaagagct gtctatcttc tgttctctct ataccttcga ttggtgttcc
    11101 aagaacttta gtcccatatt tattcaatat tccatcttgg aataatgcgg ttccacaatt
    11161 aagtgccgtt tgtcctccaa atgataacat tatagaatcg ggtctttcta tttctaagat
    11221 tttggaaacg tattctttag taataggtaa taaatacact acatcagaga atctagtatc
    11281 tgtttgaatt gtagcaatat tgggattaat taaaatggtc tttacttcgt cttctttgag
    11341 agctttgaga cattgagaac cagagtagtc aaattagcgg ttagaatcct aactttcgcc
    11401 tgcttctccg attttgattg caccactacc cagtactata acttttttta tattttctct
    11461 tcttggaatt tttttcacct cttaatttga tatttaagtt tatttattag gtaattttgc
    11521 tctcttattt aaattaacaa tttctttaaa tttatcaaaa acataattac aatcattggg
    11581 gcctggcgat gcttctggat gaaattggac ggcaattatt ggtttactat tatggattat
    11641 accttctata gttttatcat ctggattgta aaaccacggc tgaaaatctg ttgaagttag
    11701 acttgtagga tcaattccat atccatgatt ctgactcgtt atataagata aactattttc
    11761 tttattaata cacgatttat tttgtcctct atgtccatac tttagtttat acgtatctgc
    11821 accagctgac aacgcaagta tttgatttcc caaacatatt ccgagggtgg gtatagacgt
    11881 atcaatgagc tttttagctg tgttaattgt ctgcttacac atttttggat cccccggtcc
    11941 attacttatt ataactccta caggattgta agataaaatt ttttcataag ttgaatccca
    12001 tggaacttga acaactttaa aacctattct taataagtgt ctaattatac tgtatttagc
    12061 tccggtatct aataagacta aaacttcatt attagtttca ccataaattt ttacatcgtt
    12121 ggagctaact gatgacataa aattttttgt attataattt tctaagttta ttattttcct
    12181 aaaatcaatt ttatcgattg gtttatcaga tactgtcaaa actcccatca ttactccttt
    12241 ctgacgaatt atttttgtta gttcgcgtgt gtcaatgcca tatatccctg gtattttttc
    12301 ttcgtataac cattcatcta acgttttcac acaactccag tgactagcca cattcgataa
    12361 agaatgaata acaagtcctt taggttgaat tttttcagat tcaaaatatt ttggtattcc
    12421 aaattcatca cgaatattct tgtccggaac accataatta ccaactaaag gatatgtcat
    12481 acttaagatc tgcccactat aagatgggtc tgtcagactt tctatatagc cgaccatgcc
    12541 agtgttaaat acaatttctc ctgctgtttg actaggatat ccgaatccaa ttcccgaaaa
    12601 tgatttccca ttttctaata ataactttgc actaaaaccg gtatatttag gatatagtat
    12661 tggaattttt tatctataat aatacaataa aaacaaaatt taagttttgc taaaatatag
    12721 cattattttt aatatttttt ttacggttca ctttcttcct tatcctcctc atttatcaaa
    12781 aattttacgg tatcagtatc ttcctgacca ttttcatcca tcaccattag cttaaattct
    12841 aatgtgtttc cctccatatc ttcgggaatt ggaaatgaat aaatcattga attacttgtg
    12901 tttggattat cttctgaatt ttctattggt ttccataaat aactactcat tttaccttcc
    12961 ggatcaaaac tagcagatgc atccaaaaca atgttttgat cgctcatgat aacctgatcc
    13021 aatcctgcat tagctattgg ttttctatct tcgagttttg aattcgcctc aacagacggt
    13081 atttgcaggt ctgatctaaa cgaagaattc aattcagaaa actgagaaat taatttatta
    13141 ttctcattat caatcgaatt tcctattgtt ttatcgccac ttaattggtt cgtagatagt
    13201 agatttgatt tctttaaaaa tacttctctt gtcttttcct ggctagtatt tctattgctt
    13261 tcgtcatcat cctcttcctg gctagtattt ctattgcttt cgtcatcatc ctcttcctgg
    13321 ctagtatttc tattgctttc gtcatcatcc tcttcct
//
LOCUS       DASVEV010000123         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1402970, whole genome shotgun sequence.
ACCESSION   DASVEV010000123 DASVEV010000000
VERSION     DASVEV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402970"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1330..1983)
                     /locus_tag="VF222_05530"
     CDS             complement(1330..1983)
                     /locus_tag="VF222_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141920.1"
                     /translation="MHKSVVFTFGVLITSLVMLSVMPIVNNNNSILNAAMAQEYDKYG
                     DSSYSQYPTDDKKYECRTGPFEGFFVSSVEFCKHVKFEDKKDRDNKVGPQGLPGINGT
                     NGVNGTQGPPGITQLNNNNTYTIIAPIIQSEVPGGDDPDTVGEGFAVCDIGDFAISGG
                     YILNDQSPNTDNGALFSVVTDSLLGDNTWKVRTFSTGADIDVGGNVTAKCFDNPPPH"
     gene            2341..2643
                     /locus_tag="VF222_05535"
     CDS             2341..2643
                     /locus_tag="VF222_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141921.1"
                     /translation="MATNTITNKFENHLLSIKAEHQKRIRPNLHPTYNERHQKIIELI
                     DWTLEKYKEAAADTKKNQENKEVLIDNIIEELDENRDIARNKMKKAMLKDEVSIYC"
     gene            complement(2731..2871)
                     /locus_tag="VF222_05540"
     CDS             complement(2731..2871)
                     /locus_tag="VF222_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141922.1"
                     /translation="MTGKNPITGKSNTLTKISGLALYNSRANPINLKAGNMAALSTTL
                     SK"
     gene            3840..>5059
                     /locus_tag="VF222_05545"
     CDS             3840..>5059
                     /locus_tag="VF222_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141923.1"
                     /translation="MFVNSISVYLLLVLTINLHFTINTVVAEPEILSSGFKVKQILIG
                     NFESSSMAFLDEKNILVLDRDNGRVYLVNVYDSTILEEPVLDVDVSTNGFRGLLGIAI
                     DNKIFGSLTHPYVYLYYTESQTSEDGSDENKNNIVEPAGNRIYRYEFKDNKLLNPKLI
                     LNIPSLPGPHNAGGVIEIGPDNNLYILVGDLTSAFNKKYKNQAINDLDGKLPDGRGGI
                     LRINLADISSNNNDNDFYIVHDNGIIGNDYPQNLYYAYGIRNGFGIDWDPITGNLWDT
                     ENGPFFGDEINLVMPGFNSGWSKVQGIWKPEGDKMGKILLEPSPSILVDFNGKGYYSP
                     PEFIWKKSVGPSAIKFLTSKLYGSEYENDLFVGDVNLGNLYHFELSGDRTSLEFQSKI
                     LKDKIANSHDEIDDS"
BASE COUNT         1894 a          666 c          804 g         1695 t
ORIGIN      
        1 ggttttggaa gctcttctat ataacagatt ttattataat ttcaggagct ttattgtggt
       61 ataataagtt tcatatttca caatcaaaga aaattaaaat tgaataatca taataatgag
      121 gtatatttgt atatatattt agcagaaatg attacatcag aataataccg atagtggttc
      181 taaaagagga gtccaatata gaaatactac taccatgtat ttagctttta ttattagaaa
      241 cttattttaa agttatttgt gaaatattaa agtatgtgaa ttcatattac tttttcattt
      301 gaaccacaag tagcaataga agtacccatc gtaacatcag tgaggttatt agcatacact
      361 agactaacac ttcctaatag tttgaggtaa attatttaat tgtcctaatc tgaacaaaca
      421 aataagaaga tgaattggtt tacagatgtt atccagacta tgacaaatga cttcaataat
      481 tttgcctatg gtacaaaaaa aatgatataa aggatattaa taaatttctg aaaatcttat
      541 taaacgctca ttaaaagttt gaagttaatt aattatacta tatcatagcg taataacgtt
      601 cactaatata taacattcaa caacaacagt attccagtat atagtcctta cagcataaca
      661 tagaatacgg taaagtatac aagttttact agaagattat ttaaaggtcg ccaatgttaa
      721 ttaagttgtt aactgttatg cataacgaag aagaatgatg gtggaataga attccatacg
      781 aattaaaatg taaaggagaa atagaatgct atgatgaaat aaatgaactt catccaaaat
      841 atccagctat aatttataat tatgttctaa aatataaaca aaggttaaag aagattgtat
      901 caacaattaa agtgaaataa ctaagagata tcaacaacaa taataataaa attataacac
      961 acaatggaaa agcacgttat atcatgaatt gataaaatac cttctatcaa ggtattagag
     1021 ataaagttta agttaactaa caaagtaaaa tataaaattg gttttagaat ataaggtatg
     1081 tgaattcgtt agaaatttgc tcaggagaga gtaataccga tagcggttct aaaagaggag
     1141 tctattgtag agataccaga atcttgtata tagcttttat cgttagaaac ttattttaga
     1201 ataatttttg tgaaccttca aaaatttttt agatatatat tttgaaatga agataacaat
     1261 tgaaatccat tattgattaa aagataaatt tcgaatatta taaaaaataa aaaaaagtta
     1321 aggcatatct caatgtggtg gtggattgtc aaaacatttt gctgtgacat taccaccaac
     1381 atctatgtct gcccctgtac taaatgtcct aactttccag gtattatcac ccaataatga
     1441 gtcagtaact acagaaaata gtgctccatt atctgtattt ggtgactgat cgtttaagat
     1501 ataacctcct gatattgcaa aatctccaat atcacatact gcaaaccctt ctccaactgt
     1561 atctggatcg tcacctccag gtacctctga ttgaatgata ggtgcaatga tagtatatgt
     1621 attattatta tttaattgtg taattcctgg tggaccttgt gttccattaa ctccattcgt
     1681 tccgttaatt cctggtagac cttgtggacc tactttatta tccctatctt ttttatcttc
     1741 aaatttaaca tgtttacaga attcaacaga acttacaaaa aagccttcaa atggacctgt
     1801 tctacactca tatttcttat catcggttgg gtattgacta taagaactat ccccatattt
     1861 gtcatattct tgagccattg ctgcatttaa aatgctattg ttgttgttga cgattggcat
     1921 tactgataac attaccaatg aagttatcaa aactccaaag gtaaaaacta ctgacttgtg
     1981 catatttctt gtttcaaaca aaaatatatt tgaactgcga ataatagtag aataagatat
     2041 tctataaagc agaataggga atgacaatta aatttcttat taactaatca tattattcaa
     2101 aataatgcgg aaaaatgtta caaattctaa actttttgtt gttgttattt ttataatctc
     2161 aattacttga attaaataac taaatgtttg tatccgtagg agtctagcct ttccctctct
     2221 ctccctctct taatgtatct atcaaattga taaataacct ttttgtgtat tttgtaaaaa
     2281 ttgccaaatc ttgacccgtg gataaattaa aacaatagta ataaaaatga agaacatatg
     2341 atggctacaa atacaataac aaataaattt gaaaatcatt tgctatctat taaagctgaa
     2401 catcaaaaaa ggatacgacc taatttgcat cctacctata acgaacgtca tcaaaagatt
     2461 atagaattga tagattggac tttggagaaa tataaagaag cagcagccga tacaaaaaag
     2521 aaccaagaga ataaagaagt attaatagat aatatcatcg aggaactgga tgaaaatagg
     2581 gatatagcta gaaacaaaat gaaaaaagct atgctaaaag atgaagtgtc tatttattgt
     2641 taagaggaaa atactatgga ttatattctt ttcatgattc gtgaattgac aggtaaattg
     2701 tattaaaaaa ataaaaaaaa ttatggcttc ttatttagac agtgttgtac ttaatgcagc
     2761 catattacct gccttcaagt ttatgggatt agctctacta ttataaagtg ctagtccact
     2821 aattttcgtc aatgtgttac tctttccagt tattggattc tttccagtca tttctccatc
     2881 taattgaatt gtaaacaatg cttgattaga acttattttt ttaaaatatt ccgtattaag
     2941 attataaatt aaaatttacg gttctgttga ttctaaaggt gtttaaaatg ggtgttaaat
     3001 taggagccgc aataacaccc actatgttta catcatctat gaatagatag tatagatgtg
     3061 caataatata tgtacaataa aaaataaaaa gattaaagat ttttgggtgg taaaatttta
     3121 tttaagctgc tggtttattt gagtctaggc ttctttgtag agttgtctaa aagtatcata
     3181 ggcaatgcca aagcattatt attaaaatta taatattgaa tgaaatgaaa agataaactc
     3241 tatgctaatg aaatgtagtg ggccctagaa aactacgggc ccatcgggag ttgaatagcc
     3301 atggtttcag tgttcaatac ctaacaaaat ttaatattag taatttttag atattttatc
     3361 tacaagtaga aggatttcct atcaatcttt atctttaact atttgtctgt gtataataaa
     3421 tggcatcttt caaatgtaaa atctgcaata tgaactttga agataaaaga cgtttagaaa
     3481 atcataaaaa agtacatgga agaaaatcaa aagtgtccga atatggtaat cctgaattta
     3541 atatagaccg tctaagagga taaaaataga agaaaaagat gaataatatc caagtgtgtt
     3601 gtattgcaca aaccttgata gattaatagc atttgtgaga atctctcgtc aggagttgaa
     3661 tggtcatggt ttcaatgttc aatatcacaa atagaattaa ctattaaaag acaacactta
     3721 caattttgat aatagtattc tattaatttg aatgctaaga gaaccatgcc tgtctgaatg
     3781 aaggactttt tgcctcttaa aattatattt aaattgatct aacatagcaa tagtaaaaga
     3841 tgtttgtcaa ttccatttct gtttatcttt tattagtctt aacgattaat cttcacttta
     3901 ccatcaatac tgttgttgct gagcctgaaa tactatcttc aggttttaag gtaaagcaaa
     3961 ttttgatagg aaattttgaa agtagcagta tggcttttct agatgaaaaa aatatcctgg
     4021 ttctcgatag agacaatggt cgtgtatatt tagtaaatgt atatgattca acaattcttg
     4081 aagaaccagt cttagatgta gatgtttcaa ctaatggatt cagaggatta ttaggaatag
     4141 caattgataa caagatattt ggatcgctaa cacatcctta tgtgtatctt tattatacgg
     4201 aatcacaaac aagcgaagat ggaagtgatg aaaataagaa taatattgta gaaccagcag
     4261 gaaatcgaat ttatagatat gagtttaaag ataataaact acttaatcct aaactaattc
     4321 taaacattcc atctttacca gggccacata atgctggagg tgtaattgaa ataggtccgg
     4381 ataacaattt gtatatactt gtaggtgatt tgactagtgc atttaacaag aaatacaaaa
     4441 atcaggcaat taatgatttg gatggtaaac taccagatgg tagaggtgga atacttcgta
     4501 taaatttagc tgatattagt agtaataata acgataatga tttctatatt gttcatgata
     4561 atggaataat aggcaatgat tatccacaaa atctgtatta tgcttatgga attagaaatg
     4621 gttttggtat agattgggat ccaattactg gaaatttatg ggatactgaa aatggcccat
     4681 tctttggtga cgaaataaat ttagttatgc ctggatttaa tagcggttgg tcaaaagttc
     4741 aaggtatttg gaaaccagag ggagataaaa tggggaaaat attgttagaa ccttctccat
     4801 ctattttagt agattttaat ggaaaaggat actatagtcc ccctgaattt atttggaaaa
     4861 aatctgtagg accaagtgct ataaaattct taacttctaa actgtatgga tcagaatacg
     4921 agaatgatct atttgttgga gatgtgaatt taggtaattt ataccatttt gaattaagcg
     4981 gtgacagaac atctttagaa tttcaaagta aaatattaaa agataaaata gcaaatagtc
     5041 atgacgaaat agatgattc
//
LOCUS       DASVEV010000124         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1208476, whole genome shotgun sequence.
ACCESSION   DASVEV010000124 DASVEV010000000
VERSION     DASVEV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208476"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1074
                     /locus_tag="VF222_05550"
     CDS             433..1074
                     /locus_tag="VF222_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7141924.1"
                     /translation="MININLITYILVLISLIFSVPFSYLQEDNGPDALQDIPDSSPTV
                     KPKVDVTIEGTANDDKIRGGSGDDKITGEEGYDTLEGGDGSDEIDGGEGDDMIKGGRG
                     EDKINGDEGNDRIEGHQGDDEIDGGDGNDSIKGGKENDQIQGGAGNDKLEGGEGIDEI
                     EGGDGSDSFICDIFDKIIDFNSQEGDTMSGQCNSDQKSAVNNNLNNFSNNLLG"
     gene            1393..>2219
                     /locus_tag="VF222_05555"
     CDS             1393..>2219
                     /locus_tag="VF222_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX7141925.1"
                     /translation="MCGIGGLISKDSTDIIPLTKTMMTCMNKRGPDGTGVLMDKTVYQ
                     FESPEKIKSNNSNTKLALGHIRLAIVGDKTQIQPFQSCDGKLVMEHNGEIYNYKMLKK
                     RLQKEHTFVSNTDSEVVIHLLEHYYKENGFSDLLDAIKKTVADIDGIYAIAIKAQEEN
                     KIYLVRDRLGIRPLYYGQNDNVLGFASERKGLWQIGIREPTISLLPGHCVILSDKISS
                     PIKIAEPPVNFSNKPTLTNLNDAIFAYKKALYNSIKKRSQDLSRIGIIFSGGIDSVLV
                     "
BASE COUNT          830 a          272 c          392 g          725 t
ORIGIN      
        1 tattgccttt catctcaaaa tgaaaacctt tttaataaag aatgataaga ttatctctta
       61 acattacaat taatttggta tttgtatgaa ctagagtttt aatacaaatg atcaataaag
      121 ctaaagaagt tagaacaata aggtagttaa atgtaatgaa catatatatt agaaatataa
      181 tagtgaattg gttatagttt atctatttat aacaacatag ataatgtaga aatttttcgg
      241 caattactac tttattgtaa gttttctttc acaaaagaca attatagctt attaaattcc
      301 aagtctatag aaagcgaacg aagaactcgt ttagtagaac taacttccac tactttcaaa
      361 taactttttt ttaggaaatc tttttaaaaa attattaatg tgagaatata ataccatact
      421 agatcaaaga tcatgattaa cataaatctg attacctata ttcttgtctt gatttctcta
      481 atattctccg ttccattttc atatcttcaa gaagataatg gacctgatgc tttacaagac
      541 attcctgaca gttctcctac agttaagcct aaggtagatg ttactataga aggaacagca
      601 aatgatgata aaataagagg aggaagcgga gatgataaaa ttactggaga agaaggatat
      661 gataccttgg aaggaggtga tggatctgac gaaattgacg gtggagaagg ggatgacatg
      721 ataaaaggtg gaagaggaga agataaaatt aatggtgatg aagggaatga taggatcgaa
      781 ggccatcaag gtgatgatga aatagacggt ggagacggaa atgattctat aaaaggcgga
      841 aaagaaaatg atcaaatcca aggtggggca ggaaatgata aactggaggg cggagaaggg
      901 attgatgaaa tcgagggtgg agatggatcg gatagtttca tttgtgatat atttgataaa
      961 ataattgatt ttaattcaca ggaaggagat acaatgtctg gccaatgtaa ttctgatcaa
     1021 aaatcagctg ttaataataa tttaaacaac ttttctaata acctacttgg atagtcaatt
     1081 acatactaaa tgagcttgaa gatttatttt tcttacttta ttttattaat ttaacgttat
     1141 atttgatcaa aatcttgtta tctttgtaat tttattaaaa aaatttatgt atctctgtat
     1201 tattttactt attattataa tttatgaaaa ttatttgtaa ttttttttcc aactaaaata
     1261 tgtgttcttt aattataatt tagagtaggg atctaaaata taatgtgggt ttaaattatt
     1321 tatatatcct aaattaggtt acatataatt ttgtttataa agatttatta agcatgagaa
     1381 caatattaaa ttatgtgtgg aataggcgga ctaatcagta aagattctac tgatattata
     1441 cctctaacca aaacaatgat gacatgtatg aacaaaaggg gtccagacgg gactggagta
     1501 ttgatggata aaacagttta tcaatttgaa tcaccagaaa aaataaaatc taataactct
     1561 aatactaaac tagctttagg tcacattcgc cttgcaatag tgggagataa aactcaaatt
     1621 caaccatttc agagttgcga tggtaaattg gtaatggaac acaatggaga gatttacaat
     1681 tacaaaatgc ttaaaaaacg actacaaaag gagcatactt ttgtatcaaa tacagatagt
     1741 gaagtagtaa ttcatttact agaacactat tataaagaaa atggtttttc tgacctttta
     1801 gatgcaataa agaaaacagt cgcggatatc gatggaatat atgctattgc tataaaagct
     1861 caagaagaaa ataaaattta tctcgttaga gatagattgg gtataagacc actttattat
     1921 ggtcagaacg ataatgtgct aggttttgct tccgagcgga aaggtctttg gcaaataggg
     1981 ataagagagc ctactatctc attattgcct ggacattgtg ttattttatc tgataaaatt
     2041 tcttcaccta ttaaaatagc tgaaccgcct gtcaacttct ctaataaacc tactctaact
     2101 aatcttaatg atgcaatatt cgcctataag aaggctttat acaattcaat aaaaaagaga
     2161 tctcaagatc tgtctagaat tggtataatt ttttctggtg gtatagatag cgtactagt
//
LOCUS       DASVEV010000125         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_433109, whole genome shotgun sequence.
ACCESSION   DASVEV010000125 DASVEV010000000
VERSION     DASVEV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433109"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VF222_05560"
     CDS             complement(<1..898)
                     /locus_tag="VF222_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141926.1"
                     /translation="MRLQYIGLSFIIGSAIILYFYGQQDLSYFLNFAYSETSEILNLS
                     NEIINSTFPQIKTHNNNIYVVWSGNVGHGVNNEIQSDIFFTKSENKGNSFGEPINLSN
                     NPGSSFNPQSEISSNNMYVVWEDDTLSDAETHGLSANTSILFTKSKDNGTTFLPAVPL
                     SYINADSSNPDITTLNGNTVVVVWQDNLDESSKILFKKSISNNNNNNEFSDQQNISNN
                     QENNFEISPQIITSNNTINIIWDDFSPKEESSHIIKRSSIDGGNTFGHVMQLSNYSEF
                     AINDISNVYNNNVYIVWQGNFKG"
     gene            1611..1976
                     /locus_tag="VF222_05565"
     CDS             1611..1976
                     /locus_tag="VF222_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7141927.1"
                     /translation="MLHYQISVLDIQNVTGIHIHQGKSEEKGDVIVNIYEPKEKILLN
                     QNGTNLSQIESSSITIGGNTQSFFIASGTINNSDLKGPLLGKNIADLINLMKNGDTYV
                     NVHSESHPDGEIRGQIMKS"
BASE COUNT          704 a          279 c          263 g          759 t
ORIGIN      
        1 gtcctttgaa atttccttgc catactatat acacattatt attgtatacg ttacttatat
       61 cattaattgc aaattcagaa taattactta attgcataac atgcccaaaa gtattaccac
      121 catcaatact acttcgtttt attatatgtg aactttcttc ttttgggcta aaatcatccc
      181 atatgatatt aatcgtatta ttggaagtta ttatttgtgg tgaaatctca aaattatttt
      241 cttgattgtt acttatattt tgttggtctg agaattcatt attattatta ttattagaaa
      301 tacttttctt gaataatatt ttgctagatt catctaaatt atcttgccat actacaacta
      361 cagtgtttcc attgagggta gtaatatctg gatttgatga atctgcatta atgtaactca
      421 gaggaacagc aggcaaaaat gttgttccgt tatctttgct ttttgtaaat aaaatactag
      481 tatttgcact tagaccatgt gtttctgcat ctgacaatgt atcatcctcc caaacaacat
      541 acatgttgtt ggaagatatt tcggattgcg ggttaaatga gcttccagga ttattactta
      601 aattaatagg ttctccaaaa ctattgcctt tattttcact ctttgtaaaa aatatatctg
      661 attgaatctc attatttact ccatgaccta cattaccact ccatacaaca taaatattat
      721 tattatgagt tttaatttgt ggaaatgtag aatttataat ttcattgctc agattaagaa
      781 tttctgaagt ttcagaatat gcaaaattta gaaaatatga taaatcctgc tgaccgtaaa
      841 aatataatat aattgctgaa ccaataataa aagataatcc tatatattgt agtctcatag
      901 tagtctatag ttattaccta ccaagtcttt aatattacta ttagacatat ttgccattgg
      961 agctgcttta ctaatgttat tcatggttgc attttgtgat ccctgtgcat atacttctag
     1021 attgtatcaa tctatgactg taattcctat tgtataaatt ataattatta atgctataga
     1081 atagttgaac ctatttttta gcatatttgt ttataaaatc tagaaagtta tatttaagta
     1141 taataatttt ttacaatttg ttattgatta ttttttaata atttttcaat taaagtaatc
     1201 acactatttg atgtattctt tctagaagac ataaaataat tacaagctat tttctcatgt
     1261 acttttatga taccttttat aagataatcg aaagaagaac atatagttta tatgatttcc
     1321 tacaattagg aacctttaaa atatagattt gttgtagagt attaaatatt taatttaacg
     1381 ttgttaacga aaatataaat aaaactaatt ctaacttttg cttgccatga ttaaactaaa
     1441 aaaagatggc tttcaaacca tattcttttc aatggttctg gccacctcaa taattttatt
     1501 tggaagtgcg acattcgttt ttagccaaac tccaaatgag ggaaaaccta ttgatcacag
     1561 caatataaat caaaatttca acttcttttg agcgacttca taataacaca attttgcatt
     1621 atcaaataag tgttttagat attcaaaatg taactggaat tcatattcat caaggtaaat
     1681 cagaagaaaa aggtgatgtt atagtaaata tatacgagcc caaagaaaag atattactaa
     1741 atcaaaatgg aacaaatctg tcccagattg agtctagttc aataacaatc ggtggaaata
     1801 cacagagttt ctttattgct agtggaacaa taaataattc agatcttaaa ggacctttat
     1861 taggaaagaa tatagctgat ttaataaact tgatgaaaaa tggagatacg tatgttaatg
     1921 ttcattcaga atcccatcca gatggagaaa ttagaggtca gataatgaaa tcttaaatta
     1981 atcacgtttt ctatttattt ttaaa
//
LOCUS       DASVEV010000126         6477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1015018, whole genome shotgun sequence.
ACCESSION   DASVEV010000126 DASVEV010000000
VERSION     DASVEV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015018"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /gene="thsB"
                     /locus_tag="VF222_05570"
     CDS             complement(<1..1434)
                     /gene="thsB"
                     /locus_tag="VF222_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX7141928.1"
                     /translation="MSIQQGSAGGVPVLILKEGSTQTKGRDAQKNNITAAKLIAEIVR
                     SSLGPRGMDKMLVDTLGDVTITNDGATILKEIDVQHPAAKMMVEISKATDNEVGDGTT
                     SVVVFAGALIEKAEDLITNDVHPTIIVDGYRKSAQKAIEILNKIAQKIDGGNEKSDLL
                     KIAKTAMQTKLVSKEAPELAELVVKAVLQVSDKPDSKYKVDIDDVKVEKKAGGSLRDT
                     TLIQGIVLDKEVVHGGMPKRIEKAKIALLNCALEIEKTEFDAKINISSPDQMKMFLEE
                     ENRMLKSMVDKIKNTGANVVICQKGIDDIAQHYLAKSGILTVRRVKESDMTRLARATA
                     ARVVNNIDDLLPDDLGNADLVEERKVETDKWVFVEGCKHPKSVTILIRGGSQRVVDEA
                     ERSIHDAIMVTKDVLEKPFIVAGGGSPEAYVATKLREWTSTLSGREQLAAEKFADALE
                     VIPITLAENAGMDPIDTTTDLRAKQSKG"
     gene            complement(1567..2049)
                     /locus_tag="VF222_05575"
     CDS             complement(1567..2049)
                     /locus_tag="VF222_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7141929.1"
                     /translation="MSDKKTVSALVTGGEASAGPPLGPALGPLGINVLQVVNTINDKT
                     KDFSGMKVPVKVEVDPETKKFTVEVGIPPTTALIAKEAGITKGSGTSGKDYVGNISME
                     NIVKIARMKIDISYAIDLKNASKEIIGSCVSSGIMIENKPAKEIYADVEKGQFDSLFN
                     "
     gene            complement(2140..2796)
                     /locus_tag="VF222_05580"
     CDS             complement(2140..2796)
                     /locus_tag="VF222_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HEX7141930.1"
                     /translation="MKNKKNTDDNIDDIIEFNNDNYDKDTNELESNNTDTLSKDEKTK
                     NVKKVNSTINSQSDPSIKNNQNVDNQDSDSKFFAIRITGGQENVVANILQNKINSKRI
                     SLRSILILENFKGYVIVEAPDSNIAYDALYGVRHVRGQIRGDLPFSDIEKYLVKKPVV
                     TELSIDDTVEVIAGPFKSMKAKIIRVDYEKQEATVVLLDSPYQIPVTVDANYLKKTSG
                     "
     gene            3108..4088
                     /locus_tag="VF222_05585"
     CDS             3108..4088
                     /locus_tag="VF222_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7141931.1"
                     /translation="MDLLDRNILNEIQWCFPLKEKPFLEIATHHNILENEVMKRIHNL
                     KQMGLIRQINAIFDTRRLGYKSALIAFAVKPEKLDYIANEINKLSGVSHNYERNHEFN
                     IWFTLAVPPEKDMKEILDKMSSLDGVIKYRLLPTLKMYKIGVKLDMVNNDSSNPKPTE
                     TIKELNPEKSRLTDQDKEFIRELQKDIEIIPEPFKKSAEKLGITQKELFKKSKEFEEN
                     GIMRRFAAILRHRDAGFTANGMIVWKVPEEAVNKVGYKLAEFPQVSHCYRRPTYPDWK
                     FNIFSMIHARSIEAAEKIAEEISHIIKIYDYKILFSSREFKKERVKYFEE"
     gene            4158..4595
                     /locus_tag="VF222_05590"
     CDS             4158..4595
                     /locus_tag="VF222_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141932.1"
                     /translation="MLSSEIQSPKIESIKSLDIVDLAIMSQLAYSFKEEFLKYAQVDS
                     FTSPDPFKNEDSNTYLVLIDRENIDRIVSMIIILNDSYNSRIPLEKIMSDRLKIIQIS
                     KEKALLLKNEMMPKDTNNFYQFRQDGRVIGYIMFAFQICGQHY"
     gene            complement(4605..5279)
                     /locus_tag="VF222_05595"
     CDS             complement(4605..5279)
                     /locus_tag="VF222_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7141933.1"
                     /translation="MKLIKKLEIQSKENIINFLNNQAVGRFATIDINGFPQIIPMNFV
                     YVQAEKYRDNSCVLKQDSIYMHSYRFGEKIDNIKRNSNTGFEVDQHLAFLPSYYFHPS
                     DASQADTLYKSVVIKGQSYIIEDNEEKALALNSLMEKYQPEGNYEKLNSTMYSVQEVT
                     VIKLIPNNMKGKYKIGQQWTPSYRLKIAENILTREKENAKLIFEVMGIEILENKNLKI
                     KKEPVL"
     gene            complement(5361..6092)
                     /locus_tag="VF222_05600"
     CDS             complement(5361..6092)
                     /locus_tag="VF222_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HEX7141934.1"
                     /translation="MSFRIVVDERERNSRVPKFLIEDGAQIDFARLSVGDYIIAPEIA
                     IERKTIHDLINSIYDGRLFIQCYDLTQFYSKPILIIEGNILNLQVESSTENQINEIEI
                     SFEKILQVYDALATIALIFRIPVIHSPSANYTSKFLIILLNKAIKSGITINGPLIKRI
                     KKGNHMQLQQLSILSSLPGVGDKLAIRLLKKFKTPSRAINASVSELALIPGFGKARAE
                     KVKKILNHEFNEDITKNQQGTLNFE"
     gene            complement(6154..>6477)
                     /locus_tag="VF222_05605"
     CDS             complement(6154..>6477)
                     /locus_tag="VF222_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HEX7141935.1"
                     /translation="LQQLQQNLQAIMMQKQQIELEIVETERALEELSKTTTSDSIYKA
                     AGPLLIKSEKDTMEKELTEKKELANTRVMVLGKQELRVKENLKEVENKINQMLKGTQS
                     TTTQK"
BASE COUNT         2126 a         1040 c          870 g         2441 t
ORIGIN      
        1 acccttgctc tgttttgctc taagatcagt tgttgtgtct atagggtcca ttcctgcatt
       61 ttctgctaat gtaataggta ttacctcaag agcatctgca aatttttcag cagccaattg
      121 ttctcgtcca gataatgtac ttgtccattc tctaagtttt gtagcaacat aagcttcagg
      181 agatccacct ccagcgacta tgaatggttt ttccaaaaca tcttttgtaa ccatgattgc
      241 atcatgaata gaacgttctg cttcatctac gactctctga gatcctcctc ttattaaaat
      301 agttacagat tttggatgtt tacaaccttc aacaaatacc catttatcag tttcaacttt
      361 tcgttcttca actaggtcag catttcctaa atcatctggc agtagatcat ctatattgtt
      421 cactacacga gccgcagtag ctcttgctaa tcttgtcata tcactttctt taactctacg
      481 aactgtgaga ataccagatt ttgctaaata gtgttgagct atatcatcga tccctttttg
      541 acaaattacc acattagcac cagtattttt aatcttatca accatacttt ttaacatcct
      601 gttttcttct tcaagaaaca ttttcatctg atctggagag ctaatgttta tctttgcatc
      661 aaattcagtc ttttcaattt ccaaagcaca gtttaataat gctattttcg ctttttcaat
      721 tctcttaggc attcctccat gaactacttc cttgtctagt actattcctt gaattaatgt
      781 agtatctctt aatgatccac ctgctttctt ttctacttta acatcgtcaa tatctacttt
      841 atattttgaa tcaggcttat ctgatacttg caaaactgcc tttactacaa gttcagctaa
      901 ttctggagct tcctttgaaa caagtttagt ctgcattgct gtcttggcta ttttaagtaa
      961 atcactcttc tcatttccac catctatttt ttgtgctatt ttattaagaa tttcaatagc
     1021 tttttgagca ctttttctat atccatctac tataatagtt ggatgaacat catttgttat
     1081 aagatcctcc gctttttcaa tcaatgcacc ggcaaatact actacagacg tagttccatc
     1141 accaacttca ttatccgttg ccttgctaat ttctaccatc attttagcag caggatgctg
     1201 tacatctatt tccttaagaa tagtagcgcc atcatttgtg atggtaacat cacctaatgt
     1261 gtctactaac attttatcca taccacgtgg accaagactt gaacggacaa tttctgctat
     1321 tagtttagca gctgttatgt tattcttttg tgcatctctt cccttcgttt gagtggatcc
     1381 ttcttttaat attaaaactg gtactccacc agctgaacct tgttgaattg acattctatc
     1441 atctctccta aaattaattt ataacaatgt gtcagaactg gggatatttt aattttctgt
     1501 aatacaattt ttattatatt agattattca ttaattataa taaatattca gtaaatcaaa
     1561 caattattaa ttaaataagg aatcaaattg acccttctct acatcagcat aaatttcttt
     1621 agcaggttta ttttcaatca ttataccgct actgacacat gacccaatta tttcttttga
     1681 tgcatttttc aagtcaatag cataagatat atctatcttc attcttgcaa ttttaactat
     1741 attttccatg ctaatgtttc cgacataatc ttttcctgaa gttccagacc ccttcgtgat
     1801 accagcttct ttggctatta atgcggtagt tggaggtata cccacttcta cagtaaattt
     1861 ttttgtctca ggatcaacct ctactttaac aggaaccttc attcctgaga aatctttagt
     1921 tttatcgtta atggtattta caacttgcaa gacattaatt ccaagtggtc ccaatgcggg
     1981 tccaagaggg ggtcctgcac tagcttctcc tccagttact aaagcggaga cagttttttt
     2041 atcgctcatg tcaaaaacaa tatttttccg aaatataaat cgtcatcata tgtaattaaa
     2101 attattttta tgtttacact ttatatataa tagtataaac tatccacttg ttttttttaa
     2161 atagtttgca tctacagtaa caggaatttg atatggagaa tcaagtaata ctacagtagc
     2221 ttcttgtttt tcataatcaa cacgtataat ctttgctttc atcgatttaa aaggtccggc
     2281 tataacttct actgtatcat ctatgcttaa ctctgttact actggcttct ttacaagata
     2341 cttttcaata tcactaaaag gaagatctcc tcgtatttgt ccacgaacat gcctaactcc
     2401 gtatagtgca tcatatgcaa tatttgaatc aggagcttca acaataacat aacccttgaa
     2461 attctccaag ataagaatag aacgtaaact tattcttttt gaatttattt tattttgtaa
     2521 aatatttgca acgacatttt cttgaccacc tgtaattcta attgcaaaaa attttgaatc
     2581 agaatcttga ttatctacat tttggttatt tttaattgat ggatccgact gactatttat
     2641 agtagaatta acttttttta catttttagt tttctcatct ttagataaag tatctgtatt
     2701 attagattct agttcattag tatctttatc ataattatcg ttattgaact ctataatatc
     2761 atctatatta tcatctgtat tttttttgtt tttcattaat tataatacaa tattttacga
     2821 ttacagtata ttataaattt attttatatt tttaattatt agggatgaat aaaaagataa
     2881 aagtactttt attgttaata aatagtattg ctattaacaa ttaaatataa attgtttaaa
     2941 tccctgagaa tgtaatatta tagatattat aaactaaaat ctacagacat ttcaaattat
     3001 acattattat atttaaataa taataatgat atgataaatg cataagtatt taatagatat
     3061 tatacataat tcaacaaaaa ttgaaaacat ctatacaagt aaatcaattg gatttactcg
     3121 atagaaatat tttaaacgaa attcagtggt gttttcctct taaagaaaaa ccatttttag
     3181 agatagctac tcatcacaat atattagaaa atgaggttat gaaaagaata cataacctca
     3241 aacagatggg attgataagg caaattaacg caatctttga tactagaaga ttaggataca
     3301 agagtgcact aatagctttt gcggtaaaac ctgaaaaatt agattatatt gcaaatgaaa
     3361 taaacaaact ttctggtgtt agtcataatt atgaaaggaa tcatgaattc aacatatggt
     3421 tcactttagc agtacctcca gaaaaggaca tgaaagaaat tttggataaa atgtcttctc
     3481 ttgatggagt tataaaatat aggcttttgc caacattgaa aatgtataaa ataggcgtca
     3541 aacttgacat ggtaaataat gattcctcaa atcctaaacc tactgagacg attaaagaac
     3601 ttaatccgga aaaaagtaga ttgactgatc aagataaaga gtttataaga gaactacaaa
     3661 aagatataga aataattcca gagcctttca aaaaaagtgc agagaaactt ggaataacac
     3721 agaaagaatt atttaaaaaa tcaaaggagt ttgaagaaaa tggaataatg agacgttttg
     3781 ctgctatctt gagacataga gatgcaggat ttacagcaaa cgggatgata gtctggaagg
     3841 tgccagaaga agcagtaaat aaagtagggt acaagttggc agaatttcca caagtaagtc
     3901 attgttatag aagaccaact tatccagatt ggaaatttaa tatatttagt atgatacatg
     3961 cgagaagtat agaagctgct gaaaaaattg ccgaagaaat ttcacatata attaaaatct
     4021 atgactataa gattttattt agttcaagag aatttaagaa agaaagagta aaatattttg
     4081 aggaataatc aatttgataa tattttgaat acttatttaa taaaataaaa tatataacaa
     4141 atacttaaaa ggtaattgtg ttatcttctg aaatacaatc ccctaaaata gaatccatca
     4201 agtctttgga tatagtagat ctagcaatta tgagtcaact tgcctattct ttcaaggagg
     4261 agtttctaaa atatgctcaa gttgattcct ttacttcccc tgatcccttt aaaaatgaag
     4321 attccaatac ttatttagta ttaatcgata gagaaaatat agatagaata gtttcaatga
     4381 ttattatcct taatgattcc tataattcaa gaataccact agaaaagata atgagtgaca
     4441 gactgaaaat aatacaaatc tccaaagaga aagcattgtt actgaaaaat gaaatgatgc
     4501 caaaagatac aaacaatttt tatcagttta gacaagacgg tagggttatt ggatatatta
     4561 tgtttgcatt tcagatatgt gggcaacact attaaatgtg aaactcataa tactggttct
     4621 ttttttattt ttaaattttt attttctaaa atttcaattc ccatcacttc aaaaattaat
     4681 ttagcatttt ccttttctct agttagaata ttttcagcta ttttcaatct ataggacgga
     4741 gtccattgtt gtccaatctt atattttcct ttcatattgt tggggattaa ttttattaca
     4801 gttacttctt gtactgaata cattgttgag ttaagttttt cataattacc ttcaggctga
     4861 tatttttcca ttaatgaatt taaagctagt gctttctctt cattatcttc aattatatat
     4921 gactgtcctt ttatgactac acttttatac aatgtatcag cttgtgacgc atcagaagga
     4981 tggaaataat aggatggtaa aaaagccaaa tgttgatcta cttcaaatcc tgtatttgaa
     5041 ttccttttga tattatctat tttctcgcca aatcgataag aatgcatgta tatactatct
     5101 tgtttaagaa cgcaagaatt atctctatat ttttctgctt gaacatacac aaaattcatc
     5161 ggtattatct ggggaaagcc attgatatct atagttgcga atcttccaac tgcttgattg
     5221 tttagaaaat taataatatt ttctttggat tgaatctcaa gtttttttat tagtttcatt
     5281 agaattttat tttataataa ctttataaag agatttaaga taaaatgaat aatttatatt
     5341 ctaagattat gaaatataat ttattcaaaa tttaatgtcc cttgttgatt ttttgtgata
     5401 tcctcgttaa attcatgatt aagaattttt ttcacttttt cagctcttgc ttttccaaaa
     5461 cctggaatca aagctaattc agatactgat gcatttattg ctcttgatgg tgttttgaat
     5521 tttttaagca atcttatggc taatttatct cctacaccag gtaatgatga aagtatagat
     5581 aattgttgta attgcatatg gtttcctttt tttattctct taatcaacgg cccattaatt
     5641 gtaataccag attttattgc tttattcaat aaaataatta aaaattttga agtgtaattt
     5701 gctgaaggag aatgaataac tggtattcta aaaattaatg caatagttgc cagagcatca
     5761 taaacctgta aaattttctc aaatgaaatt tcaatttcat taatttggtt ttcagtagaa
     5821 ctttcaactt gtagattcaa aatatttcct tctattatta aaataggttt agaataaaac
     5881 tgggttaaat cgtaacattg aatgaaaagt cttccatcat atatcgaatt aattaaatca
     5941 tgtatcgtct ttctttcaat agctatttct ggtgctataa tataatcccc tactgaaagt
     6001 cgagcaaaat ctatttgtgc accatcttca attaagaatt taggaactct actatttcgc
     6061 tctctttcat ctactacaat acgaaaagac acaaataaaa ttaaatattc ggattattgt
     6121 ttctaactaa aaattacact ataaaaaata gctttatttt tgtgttgttg tagattgtgt
     6181 tccttttagc atttgattaa tcttattttc gacttcttta agattctctt ttacacgtaa
     6241 ttcttgtttt cctaacacca taactctggt atttgctaat tctttttttt cagtaagttc
     6301 tttttccatt gtatctttct cagattttat taataaaggt cctgctgctt tataaataga
     6361 atcagatgta gttgttttac ttaattcctc caatgctctt tctgtttcaa caatttctag
     6421 ttcaatttgt tgcttctgca tcataatagc ttgcagattt tgttgaagct gttgtaa
//
LOCUS       DASVEV010000127         6314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_44861, whole genome shotgun sequence.
ACCESSION   DASVEV010000127 DASVEV010000000
VERSION     DASVEV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44861"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF222_05610"
     CDS             <1..708
                     /locus_tag="VF222_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7141936.1"
                     /translation="LEMYRPDFSGFLWTTILCLDNKNPEIAKEFHPQACSIIPGAFQP
                     IIKDTKRTGIISQYHPQFNEQDLKIKVVKQELVKNPVQFENYKIIVSFGRGIKENPED
                     NIKLIEKLAKLLDAEIGISLPISKKIFHLSEKYDSIYITPNRVVGTSGRKVTPVIYIA
                     VGISGAIQHLDGMKESDFIIAINPDENSPIKDVCDVFIKGRMEDVIPVIIEELGKKSG
                     QLKEKEKEIQVNTYGKF"
     gene            695..2575
                     /locus_tag="VF222_05615"
     CDS             695..2575
                     /locus_tag="VF222_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HEX7141937.1"
                     /translation="MENFDVTIVGGGSAGLAALKHLSSLGKQSILLEAGKEVGSKNVS
                     GGILYSKKSNNGKIYNVEDIYGESFLTESPYERLITKYILNVTSKNKVFSIDLTESHN
                     YQTNFGYSVLLNKLNSWFAKEAQSAAEKLGGGIITGVHVRALRWDENRSKIIVETDEL
                     EEFETKVLIAADGVNSEIAEMVHAREKFSPSELYQGVKVLVKLPEKIIEERFDITNSD
                     EGAAHLFAGDVSLNLIGGGFLYTNRDSLSLGGVYHYDSLLKNPIEPSEIVNAMLRNPL
                     VSELIKDDVPLKEIDKTLPKEEQVRKSFAVTKLLNNWNELRQLYYSHNLKEKAIKDTK
                     YNSEDEIKSKLDNLTHKLTDEYNIKFKTNYIELEYTTKLVPDGKRGRMKKPYHKNILF
                     IGDAAGRGLFFGPRIEGLNVGIDDAARASIAIARSLDKNNFDSNYLGEFYSLSVEESP
                     YTVDMKEIDKQYLSVILNSTRKKIAPDVLGLKHKILLNLISNDGLRNFFITLINKYGY
                     TRLLSIIESEDVYFKIPIKIADKLGTVNSSTYTPQIPTIQDRVAKLCYQEDTISHIKI
                     QNSGKNFLKKMVILCPTKCYSQEGDEVILQHEGCIECGTCSKETEWRHPRGEKGVIFR
                     YG"
     gene            complement(2876..3181)
                     /locus_tag="VF222_05620"
     CDS             complement(2876..3181)
                     /locus_tag="VF222_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141938.1"
                     /translation="MDSKKKEIRIKSFQTQLFDELINQVNVWLSSKQDNTRNTFEFID
                     MKYYCNESMPSDNDFHYTAILIYKDSQEKLIKEDQEKNNSTNEEEFIAEKKKLEYRK"
     gene            complement(3451..3948)
                     /locus_tag="VF222_05625"
     CDS             complement(3451..3948)
                     /locus_tag="VF222_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX7141939.1"
                     /translation="MPIDPAFPKNHKVIGKHKNEDGRFHFVWGPGRSDAESSSHEQVQ
                     ESYKTRGEEYVPLGVHGTFVGVDWDSCIADGACIEACPVQVFQWYRSEHDLPAVEMTN
                     ATSAGTGENHDREGRKDYTDKSDPVREHDCIWCMACVTVCPTTAIKVDEANLEVHKKT
                     AETAS"
     gene            complement(4289..6172)
                     /locus_tag="VF222_05630"
     CDS             complement(4289..6172)
                     /locus_tag="VF222_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7141940.1"
                     /translation="MNGESKDLTTADIVAEALIDWKVDVVFGIPGDGINGFIEALRVR
                     QDKIKFVLVRHEESAALMSCAYAKYTGKIGVCVATSGPGAIHLLNGLYDAKADNVPVI
                     AITGSTFSDLMNSSFLQDVKLLQLFSDVASYNTMIHLPEQAEMAVDIACRTCLARKGV
                     GHINIPIDVQEKKLKGDYSKHKVPGHTSDIFDSFALPDKKLIEKAAKVLNKGNKIVIL
                     VGQGALNASREVVAVAQKLNAPIIKALLGKAVIPDEHPLNLGGLGMLGTEPASEAMSE
                     TDTLLMIGTSFPYMEYLPKPGQAKGIQIDMKPEKIGLRYPVEIGLVGDSKTVLSELLP
                     LLKDRTTKNNINDDDMKNNNNNIGISSVSLNFLQSMQKSMEKWKDILKEQSNRDDIPI
                     KPQVIANAVSEELEDNAIVSVDSGNNTVWAARFLKMRNGMKFSVSGNLATMACGLPYA
                     IAAKIAFPERQSIAFVGDGGFTMLMGEFATAVQYKLPIKIIILKNNLLGMIRWEQMAF
                     LGNPEYAVEFTPIDYTKFAEASGGKGYTIKKIDEVKSVMGMAMNKKNEFPTIVEAYID
                     PFELPFPPKINMEFPARVAESFVRGQPYTREIGTALSTDHIPERFNKFQSHLKQKQSK
                     EKN"
BASE COUNT         2080 a         1055 c          977 g         2202 t
ORIGIN      
        1 ttagagatgt accgacctga tttttctggg tttctttgga ctacaatctt atgtcttgat
       61 aacaagaatc ctgaaattgc aaaagaattt catccacaag catgcagtat cattcctggg
      121 gcttttcaac ctattataaa agacacaaaa agaaccggaa taatatctca atatcatccc
      181 cagtttaatg aacaggatct caaaataaaa gtagtgaaac aagaacttgt caaaaatcct
      241 gtccagtttg agaattacaa aattatcgtt agctttggta gaggtatcaa agaaaatcca
      301 gaggataaca ttaaacttat agaaaaatta gctaaacttc tcgatgctga aatcggaata
      361 tcattaccta tatcaaagaa aatattccat ttaagtgaaa aatatgattc tatttatata
      421 actcctaata gagtggttgg aacgagcggt agaaaagtta cacctgtaat ttatatcgct
      481 gttggaatta gcggagcgat acagcattta gacggcatga aagagtctga ttttatcatt
      541 gctataaacc ccgatgaaaa ttcaccaatt aaagatgtat gtgatgtgtt tattaaagga
      601 cgaatggaag atgtaatccc agttattatc gaagaattag ggaaaaagtc tggacaatta
      661 aaagaaaaag aaaaagaaat ccaagtgaat acatatggaa aattttgatg taaccatagt
      721 tggaggcggt tctgcaggac tagctgcgct taaacacctg tctagcttag gtaaacaatc
      781 tattttgtta gaagctggga aggaagttgg ttccaaaaat gtttcaggag gaattttgta
      841 ttctaaaaaa tcaaataatg gaaagatcta caatgtcgag gacatttatg gagaaagttt
      901 tctcacggaa tctccttacg aacgactaat tactaaatat attttgaatg tcacttcaaa
      961 aaataaagtc ttttcaatag atctaactga atctcataat tatcaaacaa atttcgggta
     1021 ttcagtactc ttaaataaat taaattcctg gtttgcaaaa gaagctcaaa gtgcagcaga
     1081 aaaactaggg ggaggaatta ttacaggagt acatgtaaga gctctaagat gggatgaaaa
     1141 tagatcaaaa ataatagtgg aaactgatga acttgaagaa tttgaaacaa aagtactcat
     1201 tgctgccgat ggtgtaaact cagagattgc agaaatggta catgcaagag aaaaattctc
     1261 cccaagtgaa ttatatcaag gcgtaaaggt tctagtaaag ctaccagaaa aaataattga
     1321 agaacgtttt gatataacca attcagatga aggagccgct cacctttttg caggagatgt
     1381 atcattaaat cttatcggtg gtggatttct ttataccaac agagactctc tttctctcgg
     1441 tggtgtgtat cattatgatt ctttactaaa aaaccctata gaaccctcag aaattgtgaa
     1501 tgcaatgtta agaaatcctt tggtaagtga attaatcaaa gacgatgttc ctttaaaaga
     1561 gatagataaa actcttccaa aagaggagca agtaagaaag tcttttgctg ttacaaagtt
     1621 actaaataat tggaatgaat taagacaact ttattattct cataatttaa aggagaaagc
     1681 tatcaaagat acaaaataca actcggaaga tgaaataaag tcaaaacttg ataatttgac
     1741 tcacaaactt actgatgaat acaatatcaa atttaagact aattatatag aacttgaata
     1801 tactaccaaa ttagttccag acggtaagcg aggaaggatg aaaaaaccat accataaaaa
     1861 tatattattt ataggtgatg ccgctggtcg aggtctattc tttgggcccc ggatagaagg
     1921 attgaatgta ggaatagatg atgcagctag agcttccatt gctattgcta gatctcttga
     1981 taaaaataat tttgattcta attatttggg agaattctat agtctctccg tggaagaaag
     2041 tccatatact gtggatatga aagaaattga taaacaatac ctaagtgtaa ttttaaatag
     2101 tacaagaaaa aaaatagcac ctgatgtttt gggattaaaa cataaaatac ttttaaatct
     2161 tatatctaat gatgggctaa ggaatttttt cataaccttg attaataaat atggatatac
     2221 acgattactt tcaattattg aatctgaaga cgtatacttt aaaattccca ttaaaattgc
     2281 ggacaagttg ggaacagtaa attcttctac ctatactcca caaataccca ctatacaaga
     2341 tagagtcgca aaattatgtt accaagagga tactatatct catataaaaa ttcaaaattc
     2401 tggcaaaaac tttcttaaaa aaatggtaat actctgtcca actaaatgtt acagccaaga
     2461 gggtgatgaa gttatattac aacatgaggg atgtattgaa tgtggtacct gttcgaaaga
     2521 aactgaatgg agacatcctc gtggtgaaaa aggtgtaatt tttagatatg gctaattttc
     2581 caatttttta tcttttttta taaaaataga ttaaattctt attatgtaag taatgtattc
     2641 tgatctaaag tttatattat tattaaaaca aatttttttt tcgtaaggga tatacatagg
     2701 atcatatttt attatatgtc tattactatt acctatttta attcatagca tttagacaat
     2761 agattatcct attgattttt gcatgtatta caatatcacc tgttaaaaat tttctcttag
     2821 attgatcatt tctttttttt tactaatttt tcagaatcct ttgttgtcat ttattttatt
     2881 ttcgatattc taatttcttc ttttctgcaa taaattcttc ctcatttgtt gaattatttt
     2941 tttcttggtc ttctttaatc aatttctctt gagaatcttt ataaattaat attgcagtat
     3001 agtgaaagtc attatctgaa ggcatagatt cgttacaata atatttcata tctataaatt
     3061 caaaggtatt tctggtgtta tcttgcttgg aacttagcca gacattcact tgatttatca
     3121 attcatcaaa taattgtgtt tgaaaggatt taattctaat ctcttttttt tttgaatcca
     3181 tcatttctaa atattcccca ttttatatat agaatgttag ttgttgttta tgaatttttt
     3241 ttgattttct tttataatta ttaggacatt aatatttgat gtagtattat aagacgacat
     3301 cactcttttt ttatacatcg taaattatat ttctataaat ctgataacat tgtgctaatt
     3361 ggacgaacca gattatataa aacctagttt ttatttaagt gaaaagaaaa cacttgatta
     3421 gaaaaataat agttagagca tgactaaatt ttaactagct gtctctgctg tttttttgtg
     3481 cacttctaaa ttggcttcat ctacctttat tgccgtagtc ggacaaaccg ttacacaagc
     3541 catacaccag atacaatcat gttctcttac tggatctgat ttatcagtat agtcttttct
     3601 tccttctcta tcatgatttt cacctgttcc tgcgcttgta gcatttgtca tttctactgc
     3661 aggtaaatca tgttcactcc tgtaccactg gaaaacttgt actggacatg cctctataca
     3721 ggctccatct gcaatacatg agtcccaatc tacccccaca aaagtaccat ggacacctaa
     3781 cggaacatat tcttctcctc tggttttgta tgattcttga acttgttcat gggatgagga
     3841 ctctgcatca gaccttcctg gaccccatac aaagtgaaat cgaccatctt catttttatg
     3901 cttccctata actttgtgat ttttgggaaa tgctggatct attggcataa ttataatcac
     3961 tttctatttc ttaaaagtgt taggggttat tcattctcaa atttagataa tgtgaatttt
     4021 atcaaataat attcttgcta aacataattt tacatcttgt taacaaaaga ctagaaggta
     4081 ttatattttg gtaatttcac accaaacttg tcatatatta gttctcctat gatctccata
     4141 gtaaaacaca aataattata tcctatttat ctccctcaac cttacccaat tatctcctat
     4201 tataatttga atatcttttt ttgataatta aattgtagta ggacgagaac atgaaaaatt
     4261 tttaatgtca ttacttggaa agatgcattt aatttttttc cttgctttgc ttttgtttta
     4321 aatgtgactg aaatttatta aatctttcag ggatatggtc cgtagatagt gccgtaccta
     4381 tttcccttgt atatggctgt cctctcacaa atgattctgc aacacgcgcg ggaaattcca
     4441 tatttatttt tggaggaaaa gggagttcaa atggatctat gtatgcttca actatagtag
     4501 gaaattcatt ttttttattc attgccatac ccattacaga ttttacctca tctatttttt
     4561 ttattgtata tcctttacct ccacttgcct cagcaaattt tgtatagtct ataggtgtaa
     4621 attctacagc atattcagga tttccaagaa atgccatttg ttcccatctt atcattccta
     4681 ggaggttatt cttcaaaatt ataattttaa ttggtaactt gtactgaact gctgtagcaa
     4741 attctcccat taacattgtg aaccctccat ccccgacaaa tgctatggac tgtctctcag
     4801 gaaatgcaat ctttgcagct attgcatatg gtaatccaca ggccattgtt gccaaattac
     4861 cagaaactga aaatttcata ccgtttctca ttttcagaaa acgtgcagcc caaactgtat
     4921 tatttccact atctacagag acaattgcat tatcttccaa ttcttccgaa actgcatttg
     4981 ctataacctg aggtttgatt ggaatatcat ctctgttgct ttgctctttt agaatatcct
     5041 tccatttttc catcgatttt tgcatagatt gtaaaaaatt caaacttaca gaagaaatac
     5101 ctatattatt attattattt ttcatatcgt cgtcgtttat attatttttc gttgttctgt
     5161 cttttaataa tggaagcaat tctgacaaaa ctgtttttga gtcaccaact aaccctatct
     5221 ccacaggata ccttagtcca attttttcgg gcttcatatc aatctgaatc ccttttgcct
     5281 gtcctggttt gggaagatat tccatatagg gaaatgatgt gccaatcata agtaacgtat
     5341 cagtttcact catagcctca cttgcaggct ctgttcccag cattcccaat ccacctagat
     5401 ttaagggatg ttcatcagga ataactgctt ttccaagaag tgctttgatt attggcgcat
     5461 ttaatttttg tgctactgcc acgacttctc ttgaggcatt taatgcacct tgcccaacta
     5521 aaataacaat tttgttacct ttatttagta ctttagctgc tttttctatt agttttttat
     5581 ctggtagtgc gaaagaatca aaaatatctg aggtatgacc aggtacctta tgtttggaat
     5641 aatcaccttt cagctttttc tcttgaacat ctatcgggat atttatgtga ccaacacctt
     5701 tgcgagcaag gcaagttcgg catgcaatat ctacggccat ttctgcttgc tcaggcaaat
     5761 gaatcattgt attgtatgat gcaacatctg aaaaaagttg taatagtttt acatcctgaa
     5821 gaaaactaga attcattaaa tctgaaaaag tgctaccagt aattgcaatt actggtacat
     5881 tatcggcctt agcatcgtat agaccattta agaggtgaat tgcccctggc ccggacgttg
     5941 caacacatac accgatcttg ccagtatatt tagcatatgc acaggacata agtgcagctg
     6001 attcttcatg tcttacaagt acaaacttta ttttatcttg tctaactctt aaagcttcaa
     6061 taaatccatt aattccatct ccgggaatac caaaaacaac atcaactttc caatcaatta
     6121 atgcttcagc tacaatatcg gctgttgtca gatccttaga ctcaccattc atgcttgata
     6181 ttaaatgtta ataatagtat ttaacaaagt tttatttttt attaaggggt aaaacaccga
     6241 gaatttgact tttgcatact tttttaatgt ttaatttctt tgaataaaac taaaagtaat
     6301 agagctaaat ctca
//
LOCUS       DASVEV010000128        11643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_822378, whole genome shotgun sequence.
ACCESSION   DASVEV010000128 DASVEV010000000
VERSION     DASVEV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822378"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF222_05635"
     CDS             <1..621
                     /locus_tag="VF222_05635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7141941.1"
                     /translation="NSVTNLIYNLGYFGIFIAAFIETIFPIIPSELIFPLAGYVVHSQ
                     GLGIEHVIIFGLAGSLGSTLGAIIIYFLALKLGRRIVLKIGKYILISESKLEKSEVWF
                     QKYGKVAVLLGRLAPGVRELISIPAGLSRMNFVEFTFFTFIGSFLWSLSLTMIGYYLG
                     NAWDKFSLESSKAFHIISLTIILSLAVILLYKFFKKRKRINQMKKK"
     gene            complement(626..1660)
                     /locus_tag="VF222_05640"
     CDS             complement(626..1660)
                     /locus_tag="VF222_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7141942.1"
                     /translation="MSNSINTPSKKVLVIGLGQLGLPVAKYIYDKGFDVYGYDINSES
                     MRRAQEKIGIKTTTSFRNFDIYIICVSTHNASDISSPQIEGLFSVINKISMEANNGSL
                     VSVESTIPHGTSKKVFELLEHRLHVVHAPHRWYSEEEEKHGVNQLRIIGGVNPCCLKL
                     GLEFYAGPSNCAAQETSDNEILNHYNEEENTNRYSNESPPNFIANSSSVPNNQQINTQ
                     VYIQSLEIPMHTVCTIEIAELTKIIENSHRYLQIAFAEELYLYCQANNILFSELRDAL
                     NTKWNVEILEPRGGIGGHCLPKDTKMFLNSSKMRSKILQSAMEVDSLYRNFMTRREEK
                     LMTSVENLKN"
     gene            1912..2706
                     /locus_tag="VF222_05645"
     CDS             1912..2706
                     /locus_tag="VF222_05645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7141943.1"
                     /translation="MSLIISTQELFELILAKQKKLRIVDTRSFNEYLNGHIPGAINIE
                     LMQYHWNDTSKQGLKGFNKQMRQLLSNIGASKKTLLVYYDNISGPSAARGVWLSLYFS
                     HKEVAMLDGGFTTWKKENKPIETETQPLRYFEIDKTIDETILADFRTITQAIKHNKKN
                     QIIDSRSFKEFSGEQIRAIRGGHIPSAVNIDWKENLSIDKFKSLAEIKKLYSNFSKNQ
                     EIITYCQGGYRAANTFVVLRELGFKNVKLYLGSWNEWGNNTTLPLE"
     gene            3822..4280
                     /locus_tag="VF222_05650"
     CDS             3822..4280
                     /locus_tag="VF222_05650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7141944.1"
                     /translation="MNKKDSKIQVKELEDDHLINSFFDTLSNLTEIGIDVYNKEFSRK
                     IFEKIKKADNIKIFVAIKDSDIVGSITAIIEQKFVHNGGKICHIEDVVTRKGFEKLGI
                     GSALVEKVLELAIQEKCYKVILNCSEYNSKFYEKLGFYKHDVGMRYNINI"
     gene            5082..5900
                     /gene="murI"
                     /locus_tag="VF222_05655"
     CDS             5082..5900
                     /gene="murI"
                     /locus_tag="VF222_05655"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7141945.1"
                     /translation="MNDLYKSIIMNYSPIVVFDSGIGSMSVIREIKRELPNENLIYFA
                     DKTNFPYGKKSQNDLFKIVHNTIKYLERFRPKLIIIASNTPSIQIIDEIRSLTTIPLM
                     GVKPPFKKAIKITKKKHIGIMATESSINSKELDKLIKSEIPQDIFVSKYNASSLIPLI
                     EDGTFLRNRKKIEHFIIQILGNIDNIDVITLSSTHLPFIIDQLNQLYPHVEFLDPAEL
                     IVKEVKKFLLNNSLNNKLKNGKMRILVSDNKQEFQEILRQIGRREKIEEVFWTF"
     gene            5980..8097
                     /locus_tag="VF222_05660"
     CDS             5980..8097
                     /locus_tag="VF222_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7141946.1"
                     /translation="MKRKIRLGIDVGGTFTKAIAIDIASGDFIGKTTVSTTHFSERGV
                     AEGIIIALRDLIKNYNIERSEIELISHSTTQAVNALLEGDTAKVGIIGMGVGLEKSNV
                     IKRTNIKNVELSLHKYLYTCYRFIDTSKYLDLNEIISTIASLKNEGAEVLVVSEAYGV
                     DDPSNEKYVMKYSDIPITAGHELTGIYGLEIRTLTAAINAGIMPKAISTAQYVENVVR
                     NENITCPIMIMKGDGGVTDIDTFKNKPILTILSGPAASVAGALLHSHILHGVFIEVGG
                     TSTNISIIKNGKPEIRYVTIMDHPTCIRSVDVRVAGVAGGSLVRIANNRIIDVGPRSA
                     HIAGLRYSCFVEPQELENARIITFKPNETDPDDYVCIETSNNIKIAITNTCAANALGL
                     INNEDYAFGNKRSAIKSLSLLGEKIGVNYQKTAEMILDFSLKKILHIMDPMIKEYHVD
                     EKKIILIGGGGGASILVPYIATKCKFNHEIAKNAEVVSSIGVAAAMIYEEIEKTIDTP
                     DSNDVSNIIENVKKRALDRGAVPESLSIQTEFVNERTLLRAIATGNVELDIGKNNRKE
                     IDEKEALDIASSILESNHVQPILNMKNYFGFTCETIKKQFFLKKRKNPIVILDKFGRI
                     RLMLDNAKVIEGSKKLKNEIDSMIRQLMDKSNEELAPQVHILDDMKIVDFSSLISPEH
                     LIDALKNEIDKANTDKITIIIKI"
     gene            8444..9337
                     /locus_tag="VF222_05665"
     CDS             8444..9337
                     /locus_tag="VF222_05665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7141947.1"
                     /translation="MFENSVVIAGPASIDLARSISRNLNVNIIEPELKIFSDGESKLR
                     IPSVTNKHCIIVQSLYPPPDRHIIQLLMVIHKCKKDNASKITVIIPYMAYARQDKAFL
                     EGEIISIAVIAQVIENFGVDEVITVDIHNEFSLSYFSINIKNVTAIPILVDYIKNNIT
                     IGNSFIVSPDAGGIKRAEKVAQLLNLPILCLKKKRDKNTGFVSINENIGMEVEGMDAI
                     LIDDMISTGGSIVKACEVLKKQKMRNITVVCTHAILLDNAFEKIMNSGVKEIISTNSI
                     PSFCSKVDISIMLSNILVNAN"
     gene            9383..10351
                     /gene="rnz"
                     /locus_tag="VF222_05670"
     CDS             9383..10351
                     /gene="rnz"
                     /locus_tag="VF222_05670"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX7141948.1"
                     /translation="MVELKIVFLGTSAAIPTYERGLSSIAIVRGDEIIIFDAGEGMQM
                     KFIRSKLGMNKKMKIFITHIHGDHCLGLLGLLQTLSLMGRTKPIEIFGEPRLQEFILI
                     NQKILNFNVNYHITVHTIRKEGILVEEKDYLVKACLADHSILAFSFILEENNRPGIFN
                     IEHAQQLGIPEGQLYSLLQRGTDIEYKGTKLFAREFTGPSRPGRKVGISGDTRPSKKL
                     KIFFKDCDVLVFESTFTTQERDKAIERFHSTALEASSLAKIAKVKKLILTHFSSRYTN
                     LKLIEMEAKINFKNIYIAHDLDVFNVPYPEKELTKKNGKAKEEKIN"
     gene            complement(10348..10773)
                     /locus_tag="VF222_05675"
     CDS             complement(10348..10773)
                     /locus_tag="VF222_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141949.1"
                     /translation="MSKKIKLSLIMYSVIGSMMFMNLGLFQEGLAQNSTHSSLTQTND
                     RDSQDVNNPTTEKDNGLANVPLVINITKNSNATDTVIDFIINQVNSSVVDISILEEVA
                     KDRIPLLIDTLKNTNASSIAAEYVLNTTKEGIFTNSTSN"
     gene            complement(11400..11597)
                     /locus_tag="VF222_05680"
     CDS             complement(11400..11597)
                     /locus_tag="VF222_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141950.1"
                     /translation="MTNMFDPKDYSSRIIDQIRKTINKSDESYERIICQICNRICYNK
                     KDLKIHLKKEHPDLPSPQTRT"
BASE COUNT         4341 a         1541 c         1816 g         3945 t
ORIGIN      
        1 aattctgtta caaatttaat ttacaacctt ggttactttg gtatttttat tgcggcattc
       61 atagagacca tttttccaat aataccaagt gagctaattt ttcctttagc tggatatgtt
      121 gttcatagtc aaggtttagg aattgaacac gtgatcatct tcggattagc tggttcacta
      181 ggttcaactc tcggggctat tataatttat tttttagcat taaaattagg aagaagaata
      241 gtcttaaaga ttggaaaata tattctaata agcgaatcta aactagaaaa atctgaagta
      301 tggtttcaaa aatatggaaa agtggctgtc ttactaggac gacttgcacc tggagtaaga
      361 gaattgatat caattccagc agggttatct agaatgaatt ttgtagagtt tacatttttt
      421 acttttattg gatccttttt gtggagttta tcgttaacta tgattggcta ttatttaggg
      481 aatgcgtggg ataaattttc tctagaatca tcaaaagcat ttcatattat atcattaacc
      541 attatcctat cgttagccgt tattttgctt tacaagttct ttaaaaaaag aaaaagaatt
      601 aatcaaatga aaaaaaaata acttcctaat tttttaggtt ctctactgaa gtcattaatt
      661 tctcctctct cctagtcata aagttgcggt ataaagaatc aacttccata gcagattgaa
      721 gaattttact tctcattttc gaagaattaa gaaacatttt tgtatctttc ggtagacaat
      781 gtcctccaat cccccctcta ggttcgagta tttcgacatt ccatttagta tttaatgcat
      841 ctctgagctc agaaaaaaga atattattag cttgacaata taagtaaagt tcttcagcaa
      901 atgctatttg taaatatcta tgagaattct ctattatttt agttaactct gctatttcaa
      961 ttgtacaaac tgtatgcata ggaatttcta aactttggat ataaacttgg gtatttattt
     1021 gctgattatt cggtactgaa gagctgttgg cgataaaatt tggcgggctc tcgttggaat
     1081 atctatttgt attctcctct tcattataat ggttcagaat ttcattgtca gaggtttctt
     1141 gagccgcaca gttagaaggc ccggcataaa actctaaacc tagctttaag caacatgggt
     1201 ttactcctcc aataatacgt agctggttaa caccatgttt ttcttcttct tctgaatacc
     1261 atctatgagg agcatgtaca acatgtaatc tatgctctaa taattcgaat actttttttg
     1321 aagtaccatg aggaatggta ctttctacag aaacaagaga accattattt gcttccatag
     1381 atattttatt tatgactgaa aataaacctt caatttgtgg agaagaaata tcagaggcat
     1441 tatgagtcga aacgcagata atataaatat caaaatttct aaaactagtt gtagttttta
     1501 ttccaatttt ttcctgggct ctccgcattg attcactatt aatatcatat ccatacacat
     1561 caaaaccttt atcataaata tacttagcaa caggtaaacc caattgacct aaaccaataa
     1621 ctaaaacctt ctttgatggt gtatttattg aattcgacat ctgtaagaaa actcttattt
     1681 tttattatat agcttggcga aaagagatcc ttgtaatttc tactaatgtt tcgtacgtga
     1741 ataaattatg aagaaaattt ttagtagttt gtataattaa ttatagataa catttcataa
     1801 taacttaaaa cttgaaaaaa gtacttaaac agtaataaaa agtctctgtt acatattaat
     1861 cttaagattt cactatcaaa cattatatta gaagataaaa taatagaatg attgagttta
     1921 attatttcta cacaagagtt atttgaatta attttagcaa agcagaaaaa actaaggatt
     1981 gttgataccc gatcctttaa tgaatatctt aatggacaca tcccaggagc aattaatata
     2041 gaactaatgc agtatcactg gaacgatact tcaaaacaag gccttaaagg ttttaataaa
     2101 cagatgagac agttattgtc aaatatagga gcatctaaaa aaacattatt agtttattat
     2161 gataatattt ctggaccgtc tgcagcacga ggtgtttggc tttcacttta tttttcccat
     2221 aaggaagttg caatgttgga tggaggattc accacttgga aaaaagaaaa taaacctata
     2281 gagacagaaa cacaaccttt gagatatttt gaaatcgata aaacaattga tgaaacaatt
     2341 ttggctgatt ttagaacgat tacccaagcg ataaaacata acaagaaaaa tcagataatt
     2401 gattcacgat ctttcaaaga gtttagtgga gaacaaatcc gagcaattag aggtggtcat
     2461 atacctagtg ctgtcaatat agactggaag gaaaacctat ctattgataa atttaaatct
     2521 cttgcagaaa taaaaaaatt atactctaat ttttcgaaga accaagagat cataacatat
     2581 tgccaaggag gctatagagc cgcaaatact tttgtcgtat taagagagtt gggttttaaa
     2641 aatgtcaagt tgtatttggg ttcatggaat gaatggggaa ataatactac acttccactt
     2701 gaataagtaa cttaaaaaaa aggtatttac ttatttaatt gtgatgattt attctttata
     2761 atgctataat gaatatatag tgctatctta tctatgtaaa aaggtattca gtatatgata
     2821 ggaaacgtct atcagttttg aaattgctga cctctataat gggaatagaa atttaactaa
     2881 tagttttaaa ctttgtatta aaaatctgaa tgtagaatgt tacattaaaa aaattttaaa
     2941 atttaactta tctataaaaa gaaaattctg ttactgcctt tattggtcca tctggatgag
     3001 gtaaaagtac gctaataagg tgtattaata gaatgcatga aatgactcct ggtgcgtatt
     3061 ctaaaggaga aatttaggaa gataaaacaa atatctatga taaaaaagtc aattcagtaa
     3121 tgataaaaag acacgtagga atggtatttc aaaagcctaa tctctttcca actcagtata
     3181 ttttttaatg ttgccacagg tttaaaatta aatggtctta aagataaaaa atcaaaacta
     3241 aattgtcttt tttaactacc attatcacga aataatgatt actccaaaat tgcaaccaat
     3301 acaataatag tttacaaata aaataattag ttaatgtagg aataatatat gctattattt
     3361 tcatacattg aaattgttct ttcactatag tgacatgatg tagtaatttt ggtgaacaaa
     3421 gagaatcatc attatttaat tgctatgaag ttgatatggc aaatttaaaa tgtcataatc
     3481 tgatacctat aatacatcat tggatttcgc taaaaaaacg gtagtaagag acatgccttg
     3541 cattctgcct attgggatat atcgtttaat acgcatttaa aaataattaa gttaataata
     3601 gacatatttt ataaggaact tatttacaat cttatataat tttaggttaa acaattcaga
     3661 attgcaatcc tgtatgataa agattactat tctaaccaaa taatcaaact atatgattga
     3721 ttaatactat acttttagtt acaaataata gttttatgat tttttttaaa tttggtaaaa
     3781 gaattttatt aatgatattt ataatatagc aaatgataaa aatgaataaa aaagactcta
     3841 aaatacaggt aaaagagttg gaagatgacc atcttatcaa tagttttttt gacactttat
     3901 caaatttaac agaaattggt atagatgttt ataataaaga attctctcga aaaatttttg
     3961 agaaaattaa gaaggcagac aatatcaaaa tttttgttgc tataaaggat tcagatatag
     4021 taggatcaat tactgcaatt atagaacaaa aatttgttca taatggagga aagatttgcc
     4081 atatagaaga tgttgtaact cgaaaaggct ttgaaaaatt aggaattggt tctgctcttg
     4141 tagaaaaagt attagaatta gcaattcaag aaaaatgtta taaggtaatt ttaaattgtt
     4201 ctgaatacaa ctcaaagttt tatgaaaaat taggttttta taaacatgat gtgggtatga
     4261 gatataatat caatatttga aacaaattaa ttttctatac tatagtagtc aatttctaaa
     4321 attgattcaa atggttaaat taacactatt cataaagtat aattttaatt agcgttgtat
     4381 tgcgttaggt gacatcgaaa gtattatagt atatattttt gtatttagtt ttgttttaaa
     4441 tagttcaaca taccaggtga ctgcaatagt tagttatcac ttttttgtta cttatggatt
     4501 agtatctata taagataaat attgctctat atattttatt aacaaatagc gaaattagca
     4561 tagtttgttt acaactaact aagataaatc ttccaatatc tacttacaaa ttctctttca
     4621 cctttctcga tctaaaataa atttagaacg ataaatatta tagacgtaaa agtattgtat
     4681 tggaaacaag aacaaatcgc tgttatattt tgtttcatca atatactgct gaataaggtt
     4741 cagatagaat caataaattt ctctgaaaaa ttgacagaag aaccattaaa gaacctcaat
     4801 actgtaatat tttcttaatt taattgtacc taatccattt tttttaaaac ttttggtcct
     4861 cctgtcacat cagtaaaatg ttcgaactta tactagagag ataggagaat taatcatcga
     4921 agaaaatgat atatcgatca gaaaccttaa aaaaaaaggt ttaatgaata gaattaatat
     4981 tagattatct tttttactgt tcaaattttg ttacattata tgtaattttt cttgatgaaa
     5041 aaaactattt ggatatctag tatattcttt gaagaattat attgaatgat ttatataaat
     5101 ctataataat gaattattct cctatagttg tatttgattc tggtatcgga tcaatgtcag
     5161 tgataagaga aataaaaaga gaacttccca atgaaaatct aatttatttt gcagataaaa
     5221 caaactttcc atatggaaaa aagtcacaaa atgacctatt taaaattgtc cacaatacga
     5281 ttaaatattt agaaaggttt cgtccgaaat tgataataat tgcttcaaat acaccttcta
     5341 tacaaattat tgatgaaatc agatcactta ctactattcc cttgatgggt gtaaaacctc
     5401 catttaaaaa ggccataaaa ataaccaaaa aaaaacacat aggaataatg gcaacagagt
     5461 cttcaataaa cagtaaagaa ttagataaat tgataaagtc tgaaatccct caagatattt
     5521 tcgtttctaa atataatgca tcttctctaa tccctttgat agaagatgga acttttttga
     5581 gaaataggaa aaagattgaa cattttataa tccaaatact tggaaatatt gacaatattg
     5641 atgtaataac attatcgagt acacacttac catttataat agatcagcta aatcagttat
     5701 atccccatgt agaattccta gatcccgccg aattaatagt taaagaggta aaaaaatttt
     5761 tattaaataa tagtctaaat aataaactta aaaatggcaa aatgagaata ttagtttctg
     5821 ataataaaca agaatttcag gaaattttaa gacaaattgg tagaagagaa aaaatagaag
     5881 aagtcttttg gactttttaa atataaccaa aagaataaat atatattaat agattcacag
     5941 ggtaaagtca gtttaggcat acctaacgtg tattagtcaa tgaagagaaa gattagatta
     6001 ggaatcgatg ttggtggaac ttttacaaaa gctatagcta tagatattgc atcaggggac
     6061 tttatcggta aaacgactgt ctctactaca catttttccg aacgaggtgt tgcagaagga
     6121 ataattattg cactccgaga cttaataaaa aattataata tagaaagatc tgaaattgaa
     6181 ttaatttcac acagcacaac ccaggccgtc aatgcattac ttgaaggaga tactgcaaaa
     6241 gtaggaatta tcggaatggg tgtaggattg gaaaaatcta atgtaattaa aaggacaaac
     6301 ataaaaaacg tagaactttc acttcataaa tatttataca cttgttatag atttattgat
     6361 acgtcaaaat atctcgattt gaatgaaata atttccacta ttgcttcact taaaaatgag
     6421 ggggcagagg tattggttgt tagtgaagca tatggagtgg atgatcctag caatgaaaaa
     6481 tatgtgatga agtattctga catccctatt acggcaggtc atgagttaac aggcatttac
     6541 ggattagaaa ttagaacatt aactgctgct attaatgcgg gaataatgcc aaaagcaatt
     6601 agtactgcac aatatgtaga aaatgtagtt agaaatgaga atataacctg tcctattatg
     6661 ataatgaaag gagacggagg agtaacggat attgatacct ttaagaataa acctatttta
     6721 acaatattat ccggcccagc tgctagtgtt gctggagctt tattacattc tcatattttg
     6781 catggagttt tcatagaggt aggtggaaca tctacaaata tttctattat aaaaaacgga
     6841 aaacctgaaa ttagatatgt aactataatg gaccatccta cctgtatacg ttcggtggat
     6901 gttagagtgg ctggggttgc aggtggttct ttagttagaa ttgctaataa ccgtattatt
     6961 gatgtgggac cgaggtctgc acacatagcg ggactaaggt attcctgttt tgttgaacct
     7021 caagaattag aaaatgctag aataataaca ttcaaaccaa atgaaacaga tcctgatgat
     7081 tatgtatgta ttgagacatc aaataatata aaaattgcga taactaacac ttgtgcggcc
     7141 aatgctttag gcttaataaa taatgaagac tatgcatttg gaaacaaaag atcagcaatt
     7201 aaatcgcttt ctttacttgg tgaaaagatt ggagtaaatt atcagaaaac tgcagaaatg
     7261 atattagatt tttctctaaa aaagatttta catataatgg acccaatgat aaaagaatac
     7321 cacgtggatg agaagaaaat tatacttatt ggaggaggag gaggagcgtc aattttagtt
     7381 ccctatatag caacaaaatg taaatttaat cacgaaatag ctaaaaatgc cgaagtggtt
     7441 tcttctatag gggtagcagc tgctatgatt tatgaagaga tagaaaaaac aattgataca
     7501 cctgattcaa atgatgtttc taatattatt gagaacgtta aaaaaagagc tcttgataga
     7561 ggtgctgttc cagaatctct ctcgatccaa actgaatttg tgaacgaacg cacgttacta
     7621 agagcaatag ccacagggaa tgttgaatta gatattggta agaacaatag aaaagaaatt
     7681 gacgaaaaag aggcgttaga tatagctagc tctatcttgg aaagtaatca tgttcaacca
     7741 atattaaata tgaagaatta ttttggattt acatgtgaga caataaaaaa acagttcttc
     7801 ctaaaaaaaa gaaaaaatcc aattgtcata ttagataaat tcggaagaat aagattaatg
     7861 ttggataatg caaaggttat cgaaggaagt aaaaaactaa aaaatgaaat agattcaatg
     7921 atacgccaat tgatggataa atcaaatgaa gaattggctc ctcaagtaca tattttagac
     7981 gatatgaaaa tagttgattt ttcgagttta atctctcccg aacatcttat tgatgcatta
     8041 aaaaatgaaa tagataaagc aaatacagat aaaataacaa ttataataaa gatataattt
     8101 attcgttaat taataatacg attttttttc tccaaattaa ttatttaact atacgttagc
     8161 tcaaaattat tattgttcca attatttctt aatatcacaa aggcattttt aatcatgata
     8221 tacaaattat tgtggcatgg ataattacat tttttgatcg atcatatttt atattagatt
     8281 aattattata tcatgtcaat taccaggata ttgttgaata caatccaggg atctttcgct
     8341 tatatggatg atttagttac ggtataataa ataacttttc taaatattca ctgatccttt
     8401 ataaaaaagg tttttatgat tggtccagat ttgttaatat catttgtttg aaaattcagt
     8461 ggtaatagca ggtccggctt ctatagactt ggctagaagt atatcaagaa atttgaatgt
     8521 taatattatt gaacccgagc taaaaatatt ttctgatgga gaaagcaagc ttcgtattcc
     8581 atctgttact aataaacatt gtataatagt tcaatccctt tatcctcctc cagatagaca
     8641 tataattcaa ttattaatgg ttattcataa atgtaaaaaa gataatgctt caaaaataac
     8701 agtaataatt ccatatatgg cgtatgcaag acaagacaaa gcatttttgg aaggggaaat
     8761 tattagtatt gctgtaatag ctcaagtaat agaaaacttt ggagtagacg aagtaattac
     8821 agtagatatt cacaacgaat tttctttatc atatttttca ataaatatta aaaatgttac
     8881 ggctattcca attcttgtag actatataaa aaataacata acaattggaa attcttttat
     8941 cgtctcacct gatgcaggag gaataaaaag ggcggaaaaa gttgctcaat tattaaactt
     9001 accaatattg tgcctcaaaa aaaagagaga taaaaataca ggatttgttt ctataaatga
     9061 aaatattgga atggaagttg agggaatgga tgcaattttg atagatgata tgattagtac
     9121 gggaggaagt atagtaaagg cttgcgaagt attaaaaaaa caaaaaatga gaaatattac
     9181 tgtagtgtgt actcatgcaa ttcttttaga caatgccttt gaaaaaatta tgaattcagg
     9241 agtgaaagaa atcatttcta caaattcaat tccaagtttt tgttcgaaag tagatatatc
     9301 tataatgttg tcaaacatat tagttaatgc taattagtct gattgagaac aacgttatta
     9361 atcatttaaa aagacgattc acttggtaga attaaaaata gtatttttgg gaacttctgc
     9421 agctattcca acctatgaga gaggcttatc ttccattgcc attgtgagag gagatgaaat
     9481 aataattttc gatgcaggag aagggatgca gatgaagttt atcagatcca aattaggaat
     9541 gaataaaaaa atgaaaatat tcattacaca tatccatgga gatcattgtt taggactttt
     9601 aggtctatta caaacccttt cgttaatggg cagaacaaag cctattgaga tattcggtga
     9661 gccaagatta caagaattta ttttgataaa tcaaaagata ctcaacttta atgttaatta
     9721 tcatattacc gttcacacaa tacgaaagga agggatcttg gttgaggaaa aagattatct
     9781 tgtaaaggct tgtcttgcag atcattctat tcttgctttt tcgtttattt tagaagaaaa
     9841 caatagacct ggaatattta atattgagca tgcacaacaa ttgggtatcc ctgaaggtca
     9901 actgtatagc ttattacaac gtggaactga tatagaatat aaaggaacaa aactatttgc
     9961 aagggaattt actggccctt ctcgtccagg tcgtaaagtt ggaatatctg gagatacacg
    10021 cccctcaaag aaattaaaaa tattcttcaa agattgtgat gtattagttt ttgaatctac
    10081 ttttacaact caagaaagag ataaggctat tgaaagattt cattccactg ctttagaggc
    10141 gtcttcatta gctaaaatcg caaaagtaaa aaaattaata ttaactcatt ttagttctag
    10201 atataccaat ctaaagttaa tcgaaatgga agcaaaaata aatttcaaaa atatctatat
    10261 agcacacgac cttgacgtat ttaatgttcc gtatccagaa aaagaattaa ctaaaaaaaa
    10321 tggaaaagca aaggaggaaa aaataaatta attactggta ctatttgtaa atattccttc
    10381 ctttgttgta ttaagcacat attcagcagc aatactagaa gcattagtat tctttaatgt
    10441 atcaattaac aaaggaatac ggtccttagc aacctcttct agtatagaaa tatctacaac
    10501 actactattt acctggttga taataaaatc aataacagtg tctgtagcat ttgagttttt
    10561 ggtaatgtta atgactaagg gtacattagc caaaccatta tctttttcag tagttgggtt
    10621 attgacatct tgcgaatctc tatcattagt ttgagttaga cttgaatgtg ttgaattttg
    10681 tgctagccct tcctgaaaga gtcccaaatt cataaacatc atggatccaa tcacactata
    10741 cataattaaa ctaagtttaa tttttttgct cataataata caactgatct cgaaatttat
    10801 taatatttct aatgtatttt tttataattg gtaacataaa ttttttttac taatactata
    10861 caaaaattga ctagagaata agattatata accaacggat attttctttt cttttttact
    10921 agaatttatt gattttattg aattcctata agattttagg aaaggattat taaataatat
    10981 aaaaaattaa atcagccaac attcccccct ttgttagtaa aggtttttac ttgtaataat
    11041 gtaaaaaaaa taaattttta gaactatttt attaaactta aaccttattg ttcatacctt
    11101 ataagttcaa tggtatccgt tcgaacaaaa tatattataa ttatgagctt gtttcaatac
    11161 taaataaata cgagcgtcga gctaaatctt gctcatattc cttcgggtga agtattttta
    11221 tataaataat ccctaggacc gaccttataa atgcaaaacc tagttctgtc ttatatttgg
    11281 gagctatcat atccaattaa aaattcctaa tcatatttta tgtatgttat ctatatgata
    11341 agcagttttt atgtaaccct caatgcgtga taaataacaa ttataattga ataaataatc
    11401 tatgttctcg tttgtggtga tgggagatca ggatgttctt tctttagatg aatttttagg
    11461 tcttttttgt tatagcaaat cctattacaa atttgacaaa tgattctttc ataggattca
    11521 tcagacttat tgatggtttt tcttatttga tcgataattc ttgaggaata atcttttgga
    11581 tcaaacatat ttgtcattag gcaatttcac tttataaagt tattcttatt tatttacttt
    11641 tag
//
LOCUS       DASVEV010000129         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1211210, whole genome shotgun sequence.
ACCESSION   DASVEV010000129 DASVEV010000000
VERSION     DASVEV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211210"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(987..1712)
                     /locus_tag="VF222_05685"
     CDS             complement(987..1712)
                     /locus_tag="VF222_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7141951.1"
                     /translation="MTYNSVLKNTFEVIEAERGLQELRLFKHHWPIKSWDTYKMIKII
                     SGTNRESFILDVGCYESPILPMLKRLGFINLYGCDLVLKLDNNTTLKKNSPSVYHEDY
                     KPIIEMYNDKSYQLSIRNIEDTNYSDQMFDYVTSLSVIEHGVNIEKYFREMSRIIKSN
                     GYLLTSTDYWPDKLVNNKNVLSNGTPDNIFSRYEIENLLEIADKNGLKLIEPIDFEYK
                     DKVVRWNSIGLDFTFIFFAMRKE"
     gene            2286..2741
                     /locus_tag="VF222_05690"
     CDS             2286..2741
                     /locus_tag="VF222_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141952.1"
                     /translation="MAVNTSKNSDDYKRKIDKLTSNNHNNIKTDQSTSEKVDPDERDT
                     NKESKMQVKSESIKDKISRKISKVIRETKSDKDERKTSLFGNDPSGNIKSIVLGLFAL
                     IGFIILVYSIFVAVMGEPLASVADTVFIAALIASFTLIGTIVSHLFLKK"
     gene            3842..4009
                     /locus_tag="VF222_05695"
     CDS             3842..4009
                     /locus_tag="VF222_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141953.1"
                     /translation="MTTTTVSSAGEEEQKYEEKFEIEITTQKGSSIKIETSSYNKLDS
                     LIEKAFTTIND"
BASE COUNT         1629 a          584 c          640 g         1458 t
ORIGIN      
        1 aattgccaaa gtagctttct ttttagcttc aacggatgca agttatatta caggtacgac
       61 agtttatgtt gatggtggct tgacactatc acaataaata aagagtaaaa ataatcatta
      121 acttttgttt ttatattaat aacaattgct atacactata agaaattaaa gtgtattatt
      181 catttgctaa tatatagaca acaatatatg cattttatgt tcattgaata agttaaagtt
      241 gtaccagaaa tgaagaccat agaagaaata taatatttta atgccaataa ttcatcctat
      301 catcaccata tatttggttt agaacagaac aagaacaaaa acgtgaggac aaaggtcgac
      361 tccttgccat ctacttagaa tataccacct agaaagatag atgtctatta tatataatca
      421 gaaagcagaa caatatctac tactttgtga aattaaacca tcagaatgcg aatggtgttc
      481 ctgcctggat aattctacga gacatgtcaa gtgtaaacat ctatggagtt tagaatttgt
      541 aataaggatg ggaacttaag atactgacaa gttcccagca gatgctaaaa gagatactac
      601 tacaataagt agtatacttt ctaatgaaat agtagaaatt gccctagtaa tagccagaat
      661 caaacgttat gaaactattg acgagttggc agaggccctc ataaaagacg aaattgaaat
      721 gtttagagtt actagagaca ataggaagac tatttggcga ttatatagat agattaattc
      781 atcctcatat cgacgacgat gaaaatagta tcagaaaacg agaataagaa taagaataag
      841 aagatgaagt ttcaaatgaa gaataatatt aattataata attataataa ttgtgaccca
      901 gcctcgaata aaattctgaa gactttattg aattttaatt gcttcaaata ttgtgatcta
      961 aatagaattt ttgctgaaga aaagtttcat tcttttcgca tagcaaagaa tatgaaagta
     1021 aagtccaaac ctattgaatt ccatcgaaca accttatctt tatattcaaa atctatcggt
     1081 tcgattagtt ttaatccatt tttatctgcg atctctagca aattttctat ttcatatctg
     1141 ctgaaaatat tatctggtgt accattagaa aggacatttt tattgttaac taatttatca
     1201 ggccaataat ctgtagatgt caaaagataa ccattacttt ttataattct actcatttct
     1261 ctaaagtact tttcaatgtt aacaccatgt tcgataacgg ataaagaagt gacatagtca
     1321 aacatttgat cactgtagtt tgtatcctct atgtttcgga ttgaaagttg gtacgatttg
     1381 tcattgtaca tttcaattat tggtttataa tcttcatgat atacagaagg agaattcttc
     1441 tttaaagttg tattattatc taatttcagc acaagatcgc aaccataaag attgataaat
     1501 cctaatcttt tcaacatagg taaaatagga gattcataac atcctacatc caatatgaaa
     1561 gattctctgt ttgtaccgct gattatttta atcattttat atgtatccca tgatttgatt
     1621 ggccaatggt gcttaaacag tctaagttcc tgaagaccgc gttcagcctc aattacctca
     1681 aatgtgttct ttaaaacgga attataggtc atctatgtta tctgtggaat tcgtccaaga
     1741 tttaaaaata ccgttgttta ttcgtaaaag agtacaactc aacaacctta ttgattctag
     1801 tattactcac ataaaatcta tatttaaaaa tttttggtat attcttaata ataataataa
     1861 tcattctaaa tgttattatt ttttcaactc ctaagaatta atagtattta gtgtaaatga
     1921 ctatgcattt tcgatatata tagaaatttt tgaaatatat gaatattgtg agaaaaaagt
     1981 ataaaagaga acaacatagt attagaatca atagtacaga cacatgtgtc taatatttta
     2041 tattcacttt tgatgcttga gactggatat atcaatggag tattagaata gaaaaaaata
     2101 aactcaatat tacaataata aataaactaa gataatttga gaatactggt tcgaacaacc
     2161 tatagccttc ttcattttcc ttcttggcca atcttctaga ggtttgtagt ttttactaaa
     2221 aagtttattt ttaaaaataa agttaataaa ttgtttttat actattaaat agaatactat
     2281 ttgaaatggc tgttaatacc tctaaaaatt ctgatgatta taaaagaaaa atagataaac
     2341 taacttctaa taatcataat aatattaaaa cagatcagtc tacttctgaa aaagttgatc
     2401 ctgatgaaag agacacaaat aaagaatcaa aaatgcaagt aaaaagtgaa tctattaagg
     2461 ataaaatatc tagaaaaata tcaaaagtta taagagaaac aaaaagtgat aaagatgaaa
     2521 gaaaaacatc attatttgga aatgatccta gtggtaatat taaatcaata gtattaggat
     2581 tattcgcttt aattggattt attattcttg tttattcgat attcgtagca gtcatgggtg
     2641 aaccactagc atcagtagca gatacggtat ttattgctgc attaattgcc tcttttactt
     2701 tgataggtac aatagttagt catttattct taaagaaata acttttttta atttaaatat
     2761 ctataaaaat aagaattact atataatact atttggaaaa tatgagtatt aatcctactt
     2821 gtttgcttta attttgattg ctatgatatt aatattagga ataatatttc aaagtggtca
     2881 atttttacct gctgatgctc aagaattacc aacagatcaa agtcaactgc aggatttact
     2941 atcaaaagtc tagattatgt ttatcatggt aatatggatc atatcggtta atattttact
     3001 taatggaata aatcttgcat aaggagaatt tattttactc tatgattcaa ctccctttgc
     3061 atctaaaggt catatagcag taaatttacc atgcaatccc aataatcctc tttagcccat
     3121 tttcggtatt cttataggtc tagtacctga cctggttgtc ttgcctcttg gatattgaac
     3181 aataatcaaa gccatgcaag atatgtctat catagacaat ttggatttgg agacccttga
     3241 ctgatctagt tttacaatac gtacgtgatg atgattcaat aaaactagaa gatcctcact
     3301 ctatagtaat ctcaactcat gaatcctata accaccactg aatcgttttt tgaacgtcaa
     3361 cattacgact aattaataaa ggactaccat gaataggaaa gaatctatac agggaagttg
     3421 ataattaggt gattgtctag aaaagttagg taatctaata ccttttaatt tcatgacaat
     3481 agattgaagg aaattttatt aaactctaat aatatctttt tatcaaaata attgatatat
     3541 tcttgtatga gatgtagaat agagagtaaa attacttata cactttcact tgtaatttta
     3601 aatcaagaat caggtaatca agtaaatcat acaaaagttt tttgcaaaaa atgttcagat
     3661 ttaatatata gtttgttttg taaaaaattt tatcaataaa aatgatgaga ttgaaattaa
     3721 gagtatctat gtgatccaaa ttctcaatga agcagtgaac ttctgaaata gaagaggcta
     3781 ctttgatctt cttaaatagt atattttaca cctttttgat agatagtctg aaaatgaaga
     3841 tatgacaaca acaactgtat catcagcagg agaggaagaa caaaaatatg aagaaaaatt
     3901 tgaaatagaa attacgacac aaaaaggttc gtctataaag atagaaacgt cttcttataa
     3961 taaactagac tctttaatag aaaaagcatt tactactata aacgattaag tagtgaagaa
     4021 tcaggagatg tagaacagaa actacaatat caataatctt aaattcttac tatttctaag
     4081 gatgtaaaaa atctatttga aagaatgtgc agcagcagga atagaagcta gttaagataa
     4141 tacaagttac aaatactcat aattattata taatgaggta tggtgtgtaa gggaatatgt
     4201 tcgggccata aagcaattag gccctcaaat ggtcagcgtt atttgatagg acaaaaacgc
     4261 tgtcaagttt gtcaaatttt catcaattgg gaaggtgtat tttgcccttg t
//
LOCUS       DASVEV010000130         6293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_435725, whole genome shotgun sequence.
ACCESSION   DASVEV010000130 DASVEV010000000
VERSION     DASVEV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435725"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF222_05700"
     CDS             <1..354
                     /locus_tag="VF222_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141954.1"
                     /translation="IEAEKQVIGSFQSQVDRNSNGVWDLYNPQRIAENYSVMVNNFTS
                     YLLNTSKLINNALASNMRVYNTALEQTRDNLKACAKTNTNYIQSVSGNSQNRDGNTSN
                     NNNNNNNINERTTRY"
     gene            complement(679..846)
                     /locus_tag="VF222_05705"
     CDS             complement(679..846)
                     /locus_tag="VF222_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141955.1"
                     /translation="MVIITGNLLVSDSQISKEDKDWLYLQMIQAIKKVVANSIILVFS
                     PTTLSNLVNYR"
     gene            complement(865..2862)
                     /locus_tag="VF222_05710"
     CDS             complement(865..2862)
                     /locus_tag="VF222_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX7141956.1"
                     /translation="MVEPLERPLSSYIHIQFVTADENSSVAEAVKILQPKNVEIILVT
                     SSSDGGKYVGIVTDSDILEKVVMKGEDSDLISTKSIMTSPIITLPSSSTVRQAIQLMR
                     THKIKHLPVTDSTNKENIIGTVTQEYLAEVIRISVIEKTFRGYRKVIREHYKPIFANV
                     AIILQFAGLLMVVPALLGTVLGEWQSSIGIYLAFVGMSLTGYILNTLGEKSPLNLKQS
                     SIVVVLSFVLLSLFGSLPYMYINPFWDDIDFFSLFASSFLESTSGFTTTGISTITHPE
                     DLPDSFSFYRSFTEWVGGLSFVYLVMALYYPETKLAGMKSLMSSGILRFKQLLTTISI
                     IFVIYAIIIVLLLFFLGHTDLLNSISLAFATLTSGGFIPTATVLTLENPITLIVIMGG
                     MILAALPFAFHFGIFSKEVEATREVKEILIYITIIACAIFLFMFIEKSFSEKEWLSSV
                     FHVISASTTTGFQFIDLSLLSVNGKILLIVIMLIGGTAFSTAGGIKIARLMLIIEKII
                     HKKKHASTTLMYKNNHATIPSFISATAIQFRRRIKPISNLHIAKENNKQMNCEVPLDK
                     YKFNILSDKAFKEALFVIVLYILFTFITAICIYYIANNSPSFIDAVFETASTISNTGF
                     STGITTMNLDSISKLILSFNMIMGRFEIIAILYIFISKLRR"
     gene            4551..5606
                     /locus_tag="VF222_05715"
     CDS             4551..5606
                     /locus_tag="VF222_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141957.1"
                     /translation="MHKSACKFGLSLMASLIVLSTVAAASFGNTNLFENAMASEKYSN
                     KYENSNGYANYESYGANYYQQPNYYYTQDRQYEDKQNSYTHDYNYNDKYNKYPTKENN
                     YECRTGPFEGFFVSSVEFCKSDNRKDNGYDDSDKNLQIFPANKVAELGDRWWQWIVSL
                     NNVTDPNPFTETGQAGCDVGLQDDGRLLFLVGSPSDPPLSGTFPVHDCKIKEGTSILF
                     PVVNVFCANSETDPPRNESQTRKCANEAQDRAFDLQLEINGKEIQNLERQYRVDSPPG
                     GFPFTSVPGNPANAIVGSSIGVSDGVWILLKHLPPGEHTITFSGKLDLTGLIPVEGEI
                     IVDAGATYNLKVIPKYY"
BASE COUNT         2323 a          931 c          900 g         2139 t
ORIGIN      
        1 attgaagcag aaaaacaagt aataggctct tttcaatcac aagtagatag aaatagtaat
       61 ggtgtatggg atttgtataa tccacaaaga atcgctgaaa attactccgt aatggtaaat
      121 aactttacaa gctaccttct aaatactagc aaactaataa acaatgctct agcatccaat
      181 atgagagtat acaatacagc acttgaacaa acacgtgata atctaaaggc ttgtgcaaaa
      241 acaaatacca attacataca gtcagtatca ggtaatagtc aaaatagaga cggaaatact
      301 agtaacaaca acaacaacaa caacaatatc aacgaaagaa caactaggta ttaattttaa
      361 catagtataa aaactgaaat tttgaattat tgtacctaat caatctttat tttattttat
      421 tttattttat tttattttat ttttatttta agacatgaaa ggtcaatctt agattcaggc
      481 tatactattc catccatttc aaacaaatta agttactaaa aagctaaata caagacaccg
      541 atatctctac attggactcc tcttttagaa ccgctatcgg tattactccc gacgggctcg
      601 tagttttctc gggttcacta tatttgatag taataataaa tcaagtagtc ttttgacaag
      661 ttagctattc atccaaattt atcgataatt cactaaattt gacaaggttg taggagaaaa
      721 gacaagaata atagaatttg caacaacctt tttgattgct tgtatcattt gaagatataa
      781 ccagtctttg tcttcttttg aaatttggga gtctgaaacg agaaggttac ctgttataat
      841 taccaatttt gatttgtatt tttattatct cctcaacttt gaaatgaaaa tatatagtat
      901 agctattatt tcaaatcttc ccattatcat attaaaagat aatattaatt tagagataga
      961 atccaaattc atcgttgtta ttcctgtgga aaatcctgta ttggaaatag tagaagctgt
     1021 ttcaaataca gcatcgataa aacttggact attattagct atataataga tacatattgc
     1081 agtaataaag gtaaataaga tataaagaac tataacgaac aaagcctctt taaaagcttt
     1141 atcagaaaga atattaaatt tgtacttgtc taaaggaact tcacaattca tttgtttatt
     1201 attttctttt gcaatatgta gattggaaat tggcttaatt cttctacgaa attgaattgc
     1261 ggttgctgaa ataaatgaag gtatggtagc atgattattt ttatacatca acgtagtgga
     1321 tgcatgtttt tttttgtgaa taattttttc tataattagc attagtcttg ctatttttat
     1381 accaccagca gtcgaaaatg ccgttcctcc tatgagcatt attacaatta acaaaatttt
     1441 accgttaacc gataaaagag ataaatcaat aaattggaag ccagtggttg tagaagcact
     1501 aattacatga aatacagaag aaagccattc tttttctgaa aatgatttct ctataaacat
     1561 aaataaaaaa atagcgcagg caataattgt tatatatatt aggatttctt taacttccct
     1621 tgttgcctca acctctttac tgaaaatacc aaaatgaaac gcaaaaggta atgctgcaag
     1681 tatcattcca cccataataa caattagagt aataggattt tctaaagtta gaactgtagc
     1741 agtcggtata aatccaccac tagtgagtgt agcaaacgct aacgatattg aatttagtaa
     1801 atctgtatgt ccaagaaaaa ataacaataa aacaataatt attgcatata ttacaaagat
     1861 aatacttata gtagtaagta actgtttaaa tcttaatatt ccactactca tcaaactttt
     1921 catccctgct aattttgttt ctggataata caaagccata actaaatata caaaacttaa
     1981 tccacctacc cattctgtaa aagagcgata aaatgaaaaa ctatcaggta aatcttcagg
     2041 atgagttatt gtagaaattc cagtagtcgt aaaacctgat gtactttcca agaaagaact
     2101 tgcaaaaaga gaaaagaaat ctatatcatc ccaaaatgga ttgatataca tgtatggtag
     2161 actaccaaag agacttaaca atacaaaact caatacaacg acaatagatg attgttttag
     2221 attgagtggg cttttttctc ctaatgtatt aagaatatat cctgttaagg acattcctac
     2281 aaatgccaag taaatgccaa ttgaagattg ccattcacct aaaaccgtgc ctaataatgc
     2341 aggtacaacc attagtaatc cggcaaattg caatataatg gctacatttg caaaaattgg
     2401 tttataatgt tctcgtatta cttttctata ccctctaaaa gttttctcaa taactgaaat
     2461 tcgaattact tctgcaagat attcctgtgt tactgtccca ataatgtttt ctttatttgt
     2521 actatcagta acaggcaggt gttttatttt gtgagtacgc attaattgaa tagcttgtct
     2581 tactgtagat gaactaggta atgttattat tggagatgtc attatagatt tggtagatat
     2641 taaatcagaa tcctctcctt tcattacaac tttttctaaa atatcactat ctgttactat
     2701 tccaacatat tttccaccat ctgaggatga tgtaactaga ataatttcaa cattctttgg
     2761 ttgcaatatt ttaactgctt cagcaacaga agaattttca tcagcagtaa caaattggat
     2821 atgaatatac gatgataggg gacgttctaa tggttcaacc attaacattc atcattctat
     2881 aattaattat tctaacttta tattccaagt ataaagattt atttatttaa aaatatttaa
     2941 aataatgtta atattgatgc ttttttgata ttaccaagat taagatttta aactattctc
     3001 aatttctaat aaataatgta gttagattgt gagttggaaa taatggccaa atttgtagtt
     3061 cattcactca tctaatcgaa ctcggttaca accttacctt agttagaatt catgaaataa
     3121 ctttattaaa aaatttttta atttggataa aaaagaaagg aatgataata atattatctt
     3181 gttaactttt tattataaga atgtctataa aataaatcag aagctattat ttaaaataag
     3241 tttctaacaa taaaagctaa atacatgata gtactatctt tacattggac tacctttttg
     3301 aaccgctatc ggtattactc ccgacgtatt aacaatcttt aacataattt gtaataataa
     3361 acaactatat taattatgat tatgattact aaataatgcg taatataata ttaaaaaaac
     3421 aactagttca atattttaac aattgtattg atagcaggaa catttgctgc tatatctccg
     3481 tcatctttta ttacaggagt gaatgcaacc caatgtggtg aagatgtaga atcttgtttt
     3541 gaacgacttc ttacataatc tcagtttgaa aagttgtcag cgaaattagc ctcgccagaa
     3601 ggaatacata tagacatact aggagtagat tatccaacac ttagaagttt gaagacatat
     3661 gttcggcgct tggaggttta gattcagaag gtttatctac ggccgtaaat gaacttattc
     3721 ctcagatttt tgattcaatt caactaccga ttccatctaa tgctcgtgta atagtctaat
     3781 gtatagttga tgctatcaat aggtaagtat cagatataat gtagatgtat tctcactgtc
     3841 actcaagata ggcataatat caaaagtcaa aaaaacttca aaatattctt ttttaaattt
     3901 ttaattgaaa tattaaacta tcatagtaag tcatgccact gaactattag tttaacttta
     3961 tctcatttta actcattctc atttgtattt ctaatagaat aactaactat tttaacaata
     4021 tgtttctaat aataaaagct aaatactatg taatagaacc ccactacacg gagggcacta
     4081 ctctcaatga gctacatgaa tatgtagtgg aaatctacta ttttaagctg aacgcgacta
     4141 ttgacattta ttattagaat tattatgaaa agttatatca agaataaaaa aagtcattta
     4201 aagtaaatat tatgtttagc aagtaattta tccataatct tgaggatggg tattaacatc
     4261 atcattaaca ttctccaggt atgtggaact gaatccaata ggaacattat atgttattaa
     4321 ataatttaca acttattttc acttgatatt atataacaaa ttttaatggg atttcacaga
     4381 ttatcactat attgtaagac cagatatgta ctatattttc cagtattaat attattaata
     4441 aataatagct attggattat acaataatca ttcactaagt aaaacatata gtatatgtta
     4501 ctgaatgatt ctactaactc taaatataat tagaaagtta gttaacaaat atgcataagt
     4561 cagcatgcaa attcggactt tctttgatgg cttcattgat agtgttatca acggttgcag
     4621 cagcatcatt tggtaacacg aatctttttg aaaatgcaat ggcatcagaa aaatattcca
     4681 ataaatatga aaattcaaat ggatatgcaa actatgaatc ctatggtgca aattattatc
     4741 aacaaccaaa ttattattac actcaggaca gacaatatga ggataaacag aactcttata
     4801 cccatgatta taattataat gataaatata ataaatatcc aacaaaagaa aacaattatg
     4861 agtgtagaac aggtcccttt gaaggatttt tcgtcagttc tgtagaattc tgcaaatctg
     4921 ataatagaaa agacaatgga tacgatgata gcgataaaaa cctacaaata tttcctgcta
     4981 acaaagtagc agaacttgga gatagatggt ggcaatggat agtatcatta aataatgtaa
     5041 cagacccaaa cccatttaca gaaacaggtc aagctggttg tgatgtaggt ttacaggatg
     5101 atggaagatt attgtttctt gttggttcac caagtgaccc tcctctaagt ggcacattcc
     5161 cagtacatga ttgtaaaata aaagaaggta catctattct atttcctgta gtaaatgtat
     5221 tttgtgctaa ttctgagaca gatccaccgc gaaacgaatc acaaactaga aaatgtgcaa
     5281 acgaagcaca ggatagagct tttgacttgc aattagaaat aaatggaaaa gagattcaaa
     5341 atctagagag acagtatagg gttgattctc ctcctggagg attccccttt acttcagtgc
     5401 ccggaaatcc agctaatgct atagtaggtt cttctattgg tgtatctgat ggtgtatgga
     5461 ttctattgaa acatttacca cctggtgaac atactataac ctttagtgga aaactagacc
     5521 ttacaggctt aattcctgta gaaggagaga ttatcgtaga tgcaggtgca acttacaact
     5581 taaaggtaat acctaaatat tattgacctc atctatgaca taaaaaagat atttagaaaa
     5641 tagttcattt ctaaatactc tacatttttt ttatattaaa ataagttact aataataaaa
     5701 gctgttggta gtagaatctt attatgttga ggattctaat gctaataaag tagcttgttt
     5761 aacccatgtt ttatatcttg tttttaaaat agataaaaaa atttaaatat ttattcctta
     5821 ttctattata ttagaatctt atttctaaat agatatcttt cttacggcat atgttataat
     5881 aaagtttata ttatatttcc ttttaaagta acgatatttt tagatgtgga aaatcttttt
     5941 ctattctaag gacaataata taatatgttg aaattaacaa aatccaatat ctgtatgata
     6001 ggaatgatgc taggaataac tgtaattggt gcgttgcctt ttattggatt ggcatgtaat
     6061 cattgttaga aggatacaaa cccaaagtat atccattcaa acaaaaccag aattacaaaa
     6121 cacccttaat attgaatagg taacacacca tctaaatttc ttatcctaca aaataatgaa
     6181 acatagtttt atttttaacc tttttcggcg ccgaaaaagt ctgatatttt tcttgatgat
     6241 gtgtattgtc tttaaattgt tattaatgca agaaaaagaa gataaaaaat ctt
//
LOCUS       DASVEV010000131        15252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_630098, whole genome shotgun sequence.
ACCESSION   DASVEV010000131 DASVEV010000000
VERSION     DASVEV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630098"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VF222_05720"
     CDS             <1..650
                     /locus_tag="VF222_05720"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7141958.1"
                     /translation="KGSRLYPKIERSEQFLQQHVGSKLKLVVLYVDLVDSTLMTRDFS
                     VDKLATIIQMFTQEMSVAASNFNGQILKYVGDAVIAYFPIEGNDSVSCSSAINCAFHM
                     ITIIEQAINPVLRRNNYDNLQVKIGIDASEHSIIQYVLGEKSYTDILGYGISMAAKLT
                     KLANPNQIITSHGIYICMHPSLRKRFSELEIAPRIWKYIDEKLQPGVWKSFDKMQ"
     gene            complement(1170..1460)
                     /locus_tag="VF222_05725"
     CDS             complement(1170..1460)
                     /locus_tag="VF222_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141959.1"
                     /translation="MRFLVRAQIPTEIGNKVVKNPKFLQELENYIHKVKAEASYFFEA
                     GGDRTFVFIVNIESAEMIPSIAEPLFQDIGAKVEFHPVMVLEDLKKAIEHLS"
     gene            complement(1597..2802)
                     /locus_tag="VF222_05730"
     CDS             complement(1597..2802)
                     /locus_tag="VF222_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7141960.1"
                     /translation="MNFTIFHQNSALYAADDGFFSVVPENYFDFEDDGQETSSSSTDQ
                     LQGNHQYDQQSTPSNENKYGKLTNSLSEGNYSNDINFIAAGDWNCNTETEKTIKKMTK
                     LEPELILGLGDYTFENISPQCWFDISEPINNKMKIAIGNHDLDYQSSYHQLLEHYNLQ
                     ESYYSFNFHNVHFLAISSEHPFEEGSKQYEFIKNDLEESLQDPSILWKIVFMHKPMYT
                     SATFDVEDSEELRDTFHELFETYKIDLVLSGHTQYYQRSLPLSYNNDNPTYPVVMDQN
                     NNNNNNNNNNNNNNKYSNNNGVIFVTAGTAGDDLHDIAYSLPYYVIQQGQFGFLNFDL
                     TNNGQTLISTFYDTDDNNILDKFIISKDITGKKTFSEENSEQYKNNNYFFTTHDEKSE
                     ISKKNNQLF"
     gene            complement(3004..4845)
                     /locus_tag="VF222_05735"
     CDS             complement(3004..4845)
                     /locus_tag="VF222_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7141961.1"
                     /translation="MIYDRDFFFKKRKEIGIISVVLIIISSFSLFFYQQNITEQKVKN
                     SIFAQYKDRQTESTQAISEHISSDLKLLKSILQGIADSSLLQQGLLYGDRIDKMMQEK
                     FSEVNNVTKVDTIFIADKDDIVSYEIVSEGTRSFINIDLSFREYIQETKNTLRSTFSD
                     GFKGIDTIERIALAVPIVNSSSGEFMGIIGAKIPTESFFSIYGNIQDINSQFLVVSDK
                     KGSLLAVGADKSLVGKNFFGEFVQNFTNHNPILMNLTQNLLQGIPGYAIYDYGRGEQL
                     TTNAPVFIQGKPLYFIQIVTPTTTIYSEINNVLFEEKLTMFSLIGGIIAAITALIIVL
                     IKWNSILNNEVKKRTKELQESNREIALANEELKVHDKMQKEFINVAAHELRTPIMPII
                     GLSELLYNKFITKNENNLKQEILKEYLQTLVRNAYRLQKIVEDILDVTKIESRIFRLN
                     TGLFDLNEVISNVVTDFENIIKNKNSKVQILYEPNRNKIFVNADKTRLTQAISNILDN
                     SLKFTQEGFIIITTRITNKDDKENKVIVIIKDSGIGIDNEILPRLFTKFVTKSDQGTG
                     LGLFIVKKIIEAHGGKIWAENNSNDIGSTFYFTLPIVQTSEIVIDRR"
     gene            complement(5763..6476)
                     /locus_tag="VF222_05740"
     CDS             complement(5763..6476)
                     /locus_tag="VF222_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141962.1"
                     /translation="MNRSSYFVIISFLFMLLLFSGTLSPVLGSNSVNIFPPGSKPYGL
                     TFAEHQKNFWKWALEIPANESPVNDRTGEKCTNGQSNSNSSVFYLSFNNGGISERTCK
                     VPSGKGLFIPIMQVEFSEKEVPNASIEDLRRTVTKDQDSVNSLYLKIGDKEYKYADLL
                     KYRTQTDVFEVVFPDKGIFGVIEGGVSKVIGDGFYIITEPLTKGNYSVHFKSSLICLD
                     PDCAEPNFAQDIKYNIVAG"
     gene            7045..7893
                     /locus_tag="VF222_05745"
     CDS             7045..7893
                     /locus_tag="VF222_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141963.1"
                     /translation="MISFNLITTGVYTGVKVSERPELTGEYVGGSGDLSFGKYLFLGN
                     VYRIFSFISFFSIWITTALLMINYKEKQSNAIVYWLILSIPLIYFLITYFYQYVIGNI
                     LTSYLEIDPTTVSIMLIAFLALSKPIGGLVFGIAFWKISRLMSYERNITTYMIISGFG
                     IFLIFAANQAATQIVSPYPSFGLSSITVLNIATFLMLLGIYNSATLVSANNNLRKFIH
                     NHALEPRLLHMIGKAEMEKEIQKTVKEITQDKDTLERYKEAEFEFDEKELEKYIDSVI
                     KEKKRT"
     gene            8007..8477
                     /locus_tag="VF222_05750"
     CDS             8007..8477
                     /locus_tag="VF222_05750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7141964.1"
                     /translation="MKLQYEIDINAPVDRIYEYYTNPDNIKQAWPQDIVKESESLSGS
                     KNEEGSEMKVKGEFMGRKDEMILEVTEKEQNKRLVTEQKEGPFKQWKSIQIFEKGNGN
                     NTHVRHVIDFELPTTGKIANFLSGDQAKNKIQQGLQQAAQTVKQKLESNSVSNA"
     gene            8527..8898
                     /locus_tag="VF222_05755"
     CDS             8527..8898
                     /locus_tag="VF222_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7141965.1"
                     /translation="MSNSVNNKIGYIEIPTDNIEETKKFYSNMFGWEYEKSEEGGESE
                     ENYWLIKNAGIKGAITSKREDNQTPTFYIMVESIDDFIAKSQKQGAKVVVDKKEISEG
                     FYATLQDQQQNTFGLWQSKSK"
     gene            9050..10687
                     /locus_tag="VF222_05760"
     CDS             9050..10687
                     /locus_tag="VF222_05760"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7141966.1"
                     /translation="MYKESSLQRKYQKSILIFRRDLRLKDNTALINALYSSNSVYCFF
                     ILDSNILLSNSLYSSFSLPIDPILLLKRKQRRIHLLRFLKESLLDLQKQFDKLDIEKE
                     KVGENNLSISSNLTFLLGDPVSTIKKIIQTDKEIEAIFVNRDYTPYSIERDRSIKEIC
                     RSFNIDFIDCPDILINEPEEILNSTNEPFKVFSHYYNKAIEKPLRKENSFDIKKYKIN
                     TISFDNINNSKKLKNFNSIERINNLEEFFSQIINEQSPGINNTTSLSSPTQLLLIGTR
                     KKYRELISALKYKFNKDNKQKEYINLLDNTASHLSPYLKFGICSIREVYSIIQKELGF
                     NHRLLRQLYWRDFYVYIGFHFPFVFTSPFQGRYRRKNKTNVIQWKNDPHEFEIWCNGK
                     TGFPIVDAGMRELNESGYMHNRVRLITASFLVKDLHIDWRYGERYFALKLVDYDPSIN
                     NGNWQWVASTGCDAQPFFRIFNPWLQQKKFDPNCNYIKKWIPELKDTPINLIHEWYNT
                     VNSTIDKSTKLSQIENYPRPIVEHQKEIMVTRELYNI"
     gene            complement(11341..12552)
                     /locus_tag="VF222_05765"
     CDS             complement(11341..12552)
                     /locus_tag="VF222_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX7141967.1"
                     /translation="MQTTITAAYHIGNVDNQSITSMVYNGSLGGPTLHVYPGDRIELN
                     LINNLHEPTNIHYHGLHVSPSNNSDNVFLQVAPGETQQYIVDIPIDHAPGLYWYHSHM
                     HTLAYGQVSAGLSGMIVIEGIEKLLPMPLQDIKKQTFAMRDFPVNSDPTAPIYRTVNG
                     EINPDVNITSGETQLWRLANIGSETFYKVGLPGHKFHVVAEDGYPVWKVWDTDNLVLP
                     SGKRFDVLVTPTGNGSLPLKALDYYPYPETTIATVNVQGNQKEPVKTIPSSLTSKEDL
                     NLMNITNNRVLTFSSNEEEQRFTINNKTFDHNRIDQKVKLGDVEQWRLINTDEDEHPF
                     HIHVNDFQVITVNGKPYDAHGLQDTVLIPGHGEVVIRIPFEDFVGKSVYHCHIMFHED
                     LGMMGAFEIVK"
     gene            complement(13203..13454)
                     /locus_tag="VF222_05770"
     CDS             complement(13203..13454)
                     /locus_tag="VF222_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141968.1"
                     /translation="MSNDIPLNDSNIQDIIKKEARGLGDDTDFGEVQEVLGEHVITKK
                     GTVGTDRFYFPKNLIERFDGDTVYFKITKDESKEYKRDY"
     gene            complement(14178..14462)
                     /locus_tag="VF222_05775"
     CDS             complement(14178..14462)
                     /locus_tag="VF222_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141969.1"
                     /translation="MQKSDKEEHNNRVYIGRIAVRLGKFRCQNYINEFNRCRNRATKL
                     AIIQLQQQQDKTPSLHQRIWICEDCFKVKFLNDNKFKDQLLQVINLSNKE"
     gene            complement(15063..>15252)
                     /locus_tag="VF222_05780"
     CDS             complement(15063..>15252)
                     /locus_tag="VF222_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141970.1"
                     /translation="EKLATAGEKSALYMRTIDDFAHWVTVNMMSNLDVEECTSILEDY
                     IKSNEHIIQRINKIMPLS"
BASE COUNT         5369 a         2274 c         2174 g         5435 t
ORIGIN      
        1 aaaaaggttc tagactttat cccaaaatag aaagatctga acagtttttg cagcagcatg
       61 taggttctaa actgaaactt gttgtacttt atgttgattt ggttgattct acattgatga
      121 cgagagattt ttcagtcgat aaattagcta caataataca gatgtttaca caggaaatgt
      181 ctgttgcagc ttcaaatttt aatggacaga tattaaaata tgtcggagat gccgtaatag
      241 catactttcc tattgaagga aacgattctg tttcctgtag ttccgcaata aattgtgcat
      301 ttcatatgat tactattata gaacaggcaa taaatccggt cttaagacga aataattatg
      361 acaacttgca agtaaagata ggtatagatg ctagtgaaca ttcaattatt caatatgtat
      421 taggtgaaaa atcctatacc gatattttag gatacggtat aagtatggca gcaaaattga
      481 ctaaattagc aaatcctaat caaattatca caagtcatgg aatatatatt tgcatgcatc
      541 catcactaag aaagagattt tctgaattgg agatagcgcc gagaatatgg aaatatatag
      601 atgaaaaact tcagccagga gtttggaaat ctttcgataa aatgcaataa taaaagactt
      661 attgtaagat catttcctat ttgctgaagc tcattctttg ctaatgttgc ttttattata
      721 aatttgtcaa actttggatt gtcagaaacc ttttgtaccg ataatatgtt ctaatatcat
      781 attgatttat gaaatcatct acatctcctt taaatccact aaaaactatc ggcaaactgg
      841 tcaaagaaat tgaagatgcg tatttcataa ggataaactt acatatcact ttccaacata
      901 ttctacattg aatttaatta atataaacaa tatgaatcaa tcttcaatta ttcagttatc
      961 aatgcattag aattcagaga tctagtatcg ttatctaata aagagaaaaa agcactgaat
     1021 tcgccaaggg ttattattca atgactactt tggataaagc tattaatgat tgattgattg
     1081 aagattataa aaatattatg ccatacctaa tttcttacca tcttttgtat cacaaaaagt
     1141 gctttagtac ttacatctat aacaaaactc taactcaaat gttcaattgc tttttttaga
     1201 tcttccaata ccattacagg atgaaattca actttggcac ctatatcctg aaataatggt
     1261 tctgctatag atggtatcat ttcagcactt tcaatgttaa cgataaagac aaaagttcta
     1321 tccccaccag cttcaaagaa atacgatgct tctgctttca ctttatgtat atagttctca
     1381 agttcttgta gaaattttgg atttttcacc accttattac ctatttctgt tggtatttgg
     1441 gctctaacta gaaatctcat agattggtta taattatgtt atatcttttt aaagtttcta
     1501 ttgagttaaa atactgaata gaactgttag acttattcga ttaatccaag aattattatc
     1561 ataatttact atattatcgt tctttagaat ttgatgttaa aataattggt tattcttctt
     1621 tgatatttct gatttttcat cgtgggtagt aaaaaaataa ttattatttt tatactgctc
     1681 agaattttct tcggaaaatg ttttcttccc tgtaatatcc tttgaaatta taaatttatc
     1741 taatatatta ttatcatcag tatcataaaa tgtacttatt agtgtttgtc cattgtttgt
     1801 caagtcaaag tttaaaaatc caaattgtcc ttgttgaata acataataag gaagagaata
     1861 ggcaatatcg tgtaagtcat ctcctgctgt tccagcggtc acaaatatga caccattatt
     1921 gtttgaatat ttattattat tattattatt attattatta ttattattat tattattctg
     1981 atccattaca actggatatg ttggattatc gttgttatag gaaagaggaa gagatctttg
     2041 ataatactga gtatgccctg atagaacgag atctattttg tatgtttcaa ataattcatg
     2101 aaaggtatct ctcagttctt ctgaatcctc tacatcaaaa gttgcagaag tatacatagg
     2161 tttgtgcatg aatacaatct tccataagat agatggatcc tgtaatgatt cttctaggtc
     2221 attttttata aattcatatt gtttgcttcc ttcttcaaat ggatgttcgg atgatatggc
     2281 taaaaaatgg acattatgaa agttaaagga ataataagac tcttgtaaat tataatgctc
     2341 aagtaattga tgataagaac tttgataatc taaatcatga ttacctatag caattttcat
     2401 cttattatta ataggttctg aaatatcaaa ccaacattgt ggagatatat tttcaaaagt
     2461 ataatcgcca agacctagta tgagttcagg ttctaacttt gtcattttct ttattgtctt
     2521 ttctgtttct gtattacaat tccaatctcc agcagcaata aaattgatat catttgaata
     2581 atttccttct gataaagagt ttgttaattt tccatacttg ttttcgtttg atggggttga
     2641 ttgttgatca tattgatgat ttccctgtaa ttggtcagtc gaagatgatg atgtttcttg
     2701 accatcatcc tcaaaatcaa aataattttc tggaactaca ctaaaaaatc catcatctgc
     2761 cgcatatagt gcgctatttt gatggaatat agtaaaattc aataagaaca ctaatagaaa
     2821 tagtgtaaaa ataaaatagt agttcctatt aactatcata atactactta acaaagttat
     2881 taatattctt atttaagatt tatagaaaac atttcataaa tatatttaat attaaatttc
     2941 tattattctt gtctttattc aatcatctat taataaaatt cttttagtca ttgttataag
     3001 aaattatcgc ctatctatta ctatttcact agtttggact atagggagtg taaaataaaa
     3061 agtagatcct atgtcattag aattattttc tgcccatatt ttaccaccat gagcttctat
     3121 aatcttcttt actatgaaga gaccaagacc agtgccttga tctgattttg taacaaattt
     3181 tgtaaatagt cttggtaaaa tctcattatc aatacctata ccactatctt ttattatcac
     3241 aattacttta ttttccttat catctttgtt tgttattcta gttgtgatta ttataaatcc
     3301 ttcttgagta aatttaagag aattatcaag tatatttgag atggcttgtg ttaatcttgt
     3361 tttatctgca ttaacaaata ttttatttct attaggttcg tatagtatct ggactttact
     3421 atttttattc tttataatat tctcaaagtc tgtgacaaca tttgatataa cttcatttaa
     3481 gtcaaataat cctgtattta atctaaagat tcgactttca atctttgtaa catctagtat
     3541 atcctcaaca attttttgta atctgtaagc atttcttact agggtttgaa gatattcttt
     3601 aagtatttct tgttttaaat tattttcatt tttagttata aatttattgt aaagtaattc
     3661 actaagacct ataataggca taataggagt tctaagttca tgagctgcta cattgataaa
     3721 ttctttttgc attttatcat gaacttttag ttcttcattt gctaatgcaa tttctctatt
     3781 tgattcttgt agttcttttg ttcttttctt tacttcatta ttaaggatgc tattccattt
     3841 tattaggact attatcaaag cagttattgc agcaatgata ccaccaatta atgagaacat
     3901 tgttagcttc tcttcaaaca acacattatt aatttctgaa taaattgtag tagtaggagt
     3961 aactatctga ataaaatata aaggcttacc ttgaataaag acaggagcat tagtagttaa
     4021 ttgttcaccc cttccataat cataaattgc atatcctgga ataccttgta atagattttg
     4081 tgtaagattc atcaaaatgg gattatgatt ggtaaagttt tgtacaaatt caccaaaaaa
     4141 attctttcct accaaacttt tatctgcacc aacagcaagt aaacttcctt tcttatcaga
     4201 gacaactaag aattgagaat taatatcttg tatatttcca taaattgaaa aaaaagattc
     4261 ggtaggtatt tttgcaccaa ttattcccat aaattctcca ctactactat ttactattgg
     4321 aacagctaat gcaattcttt ctatagtatc aattccctta aatccatctg aaaaagttga
     4381 tcttagagta ttttttgttt cctgtatata ctctctaaat gaaagatcta tatttataaa
     4441 acttcttgta ccttctgaaa cgatttcata actaacaata tcatctttat ctgcaatgaa
     4501 gattgtatct acttttgtca cattattaac ttcagaaaat ttttcttgca tcatcttatc
     4561 aatcctatcg ccataaagaa gaccttgctg taaaagactg gaatctgcaa ttccttgaag
     4621 tatcgattta agtaatttaa gatctgagct aatatgttca gatatcgctt gagtagattc
     4681 tgtttgacga tctttatatt gagcaaatat actattttta accttttgtt ctgttatatt
     4741 ctgttgataa aaaaataaac taaatgaaga gattattatt agaaccacag agataattcc
     4801 tatttccttt ctctttttaa agaagaaatc tctatcatat atcattataa actaattctc
     4861 tggttgggac ttgaaccttc gtccatcaaa tgtgggttct actatcaatt attaaaatat
     4921 ttaattctag ataagattag aaatgtatgc ttcaaccaat aaaataattc gtttaattcc
     4981 gtaccaataa tgtcaataac ttaatatttt tattcataat tataaaattg tcatagcctg
     5041 aacaagtaag cagtatagaa gaaggtttat agttactact aactaaacac atacatatct
     5101 atactatctg tcgtcgtcca gactatgaca aacatgtttc catatttgtt gagagaagtt
     5161 ttttttgaga gatggtattt cattcataac gcatattgta tttatcaact cttgtaaacc
     5221 tatgttgaga ctttaatcaa ctaactgtca atcaaacaat caatcaatat cattattctt
     5281 attcttagat aaatttgtaa cttgtaataa ttaatctgta aatttcctat cgtttaacaa
     5341 ccttaactaa tctaatagtt tgttaataga ttatatcctt ttacttttat tatttgcaca
     5401 acctctataa cctaaattta tttatctttt aatctgtgat tctcgcatta agactcaaaa
     5461 taaagtttat ttaatgttgt gtatggatat acaatattat tttaagtcac tcttaaaaaa
     5521 ataaatttga gaccaaattt tttaatactg acttcctctt aaaattattg ttacagaatt
     5581 agcatggagg ggatgggatt tgaacccgtt acttgcattc acgggtttag acaatctatt
     5641 aaatattcaa ctatatctaa agcctttcta agtgtaacag gattggaaag acaatgaaaa
     5701 aatgtaaaaa agaacctaaa atagaattat gataaaaaaa ggagagaatt ggtactatat
     5761 atctatcctg caactatatt gtatttgata tcttgagcaa aattaggttc tgcacaatct
     5821 ggatccaggc aaattaaact cgatttaaaa tgaaccgagt aatttccttt cgtaagcggt
     5881 tcagttatta tataaaaccc atcacctata actttagaaa caccaccttc aataactcca
     5941 aagattccct tatcaggaaa gacaacctca aaaacgtctg tttgagttct atatttgaga
     6001 agatctgcat atttatactc tttatcacct attttaagat acaaactgtt gacactatct
     6061 tgatccttcg ttacagttct acgcaaatct tctatcgatg catttggaac ttccttctca
     6121 gaaaattcta cttgcattat tggtataaag agaccttttc cagatgggac tttacatgtt
     6181 ctttcagata tacctccatt attaaaactc aaataaaaaa cagatgaatt ggaattggac
     6241 tgcccatttg tgcatttctc tcctgtacga tcgttaacag gactttcatt cgcaggtatc
     6301 tctaatgccc atttccaaaa atttttttga tgttcagcaa atgttaaacc atatggtttg
     6361 cttcctggtg ggaaaatatt tactgaattt gaacctaaaa ctggcgataa cgttcctgag
     6421 aatagtagca acataaacaa aaaagatata ataacaaagt aagatgatct attcatctat
     6481 ctatcatcac agtttagcag ctctctaata caaaaagatt atgtttgact taatgcttcc
     6541 tatctagtat aatcgttgct cacagatcat aaaaagtcgt ttagcaatag gaaatttctt
     6601 attcttatga ttgcattaat agtcactgtc ttgcttgata cttcagttgt taagatcaac
     6661 gatctcattg ataaatactt catttctctg caaagtaagt taatgttatt tgctgttaac
     6721 agttcattat gcctgctttt acaatttttg ataataaagt atttacagag cttgattaaa
     6781 agagatcaat taaataagat attaaaggtt caggcttttt ataaaatttc gttaatttct
     6841 ctatgcatat tagcaacttt gataggaatt ttgatatttc aacagttcca ttataattac
     6901 tactacacat cgatcagtat tttgataatt atgataagtt atggaacagc agcaggtttc
     6961 ctaatatggt tatctgtact atttttctca tggtataaat caaaccgtaa tttaatagtc
     7021 attttatatt ttatatcaat gttaatgata tcatttaatt taatcacaac aggtgtttac
     7081 acaggcgtca aggtgagtga aagaccagag cttactggag aatatgttgg aggttcggga
     7141 gatctatcat ttggtaagta tctattttta ggtaatgttt acagaatctt ttcatttata
     7201 tcctttttca gtatttggat tacgacagca ctattaatga tcaattacaa agagaaacag
     7261 agtaatgcta tagtatactg gcttatattg tctataccac ttatttattt tttaataacc
     7321 tatttttatc agtatgtcat aggtaacata ttaacctcat atctggaaat tgatcctaca
     7381 actgtttcta tcatgttaat agcattttta gcattaagta aacctatcgg aggattggtt
     7441 ttcggaattg cattctggaa gatatccagg cttatgagtt atgaaagaaa cattactacc
     7501 tacatgatta tttctggctt tggcatattt ttaatttttg cagcaaatca agcagcaaca
     7561 caaattgtta gtccctatcc atcttttgga ctttcatcaa taacagtttt gaatatagcc
     7621 acatttttga tgttgttagg tatttacaat tctgcaacac ttgtttcagc aaataacaat
     7681 ctacgtaaat ttatacataa ccatgccttg gaaccaagac tgttacacat gataggaaaa
     7741 gcagaaatgg agaaagaaat tcaaaaaact gtcaaagaaa ttactcaaga caaagatact
     7801 ttagaaaggt acaaagaagc agagtttgaa tttgatgaaa aggaattaga aaaatatata
     7861 gattctgtaa taaaagaaaa aaaaagaaca taaatagtaa tcaatcactc aactcgaaag
     7921 tatagacaag aaagtaaaat cctagagcct aactcttttg aaatttatta tataactatt
     7981 attaaggata ttgaaatctt tttcttatga aactgcaata tgaaatagat attaatgcac
     8041 cagtagacag aatatatgaa tattatacca atccagataa cataaaacaa gcatggcctc
     8101 aagacattgt aaaagaatca gaatcattat ctggttctaa aaatgaggaa ggctctgaaa
     8161 tgaaagtaaa aggagaattt atgggaagaa aagatgaaat gattttagaa gtaactgaaa
     8221 aagagcaaaa taaaagacta gtaactgaac agaaagaagg accatttaag caatggaaaa
     8281 gtattcaaat ttttgagaaa ggaaatggta ataatacaca tgtaagacat gttattgatt
     8341 ttgaattacc aacaacaggt aaaattgcca attttctttc aggagatcaa gcaaagaata
     8401 agatacaaca aggtttgcaa caagcagcac aaacagtaaa acagaaacta gaatcaaatt
     8461 cagtttctaa tgcataaaaa gccagaccta cagagtataa taaaaaaata agagggaaga
     8521 agaatcatga gtaattctgt taataataaa ataggatata tagaaatacc tacagacaac
     8581 attgaagaaa caaaaaaatt ctattcgaat atgttcggtt gggaatacga gaaaagtgaa
     8641 gaaggaggag aaagtgaaga aaactattgg ttaataaaaa atgcgggaat aaaaggagca
     8701 ataacttcaa aaagagaaga taatcaaact cctacatttt atattatggt agagtcaata
     8761 gatgacttta tagctaaatc tcaaaagcaa ggtgcaaagg tggtagtcga caaaaaggaa
     8821 atatctgaag gtttttatgc tacattacaa gatcagcaac aaaatacatt tggtttatgg
     8881 caaagcaaga gtaaataatt caattaaatt atgtctaatg acgaataata tattataact
     8941 tctttcttta aacaatagca ataacaaata catattgtta gggtggttag gcactaacat
     9001 aaactgttca tatataatat attataataa ataattaatc aattttcata tgtataaaga
     9061 aagcagctta caacgaaagt atcaaaaaag tattttaatt tttagacgcg atttacgatt
     9121 aaaagataat acggcattga taaatgcttt atattcttca aattcagttt attgcttttt
     9181 tattttagat agcaatattc tgttgtcaaa ctcattgtat tcttcttttt ctttaccaat
     9241 agatcctatt ttgcttttaa aacgcaagca aagaagaata catttgctaa gattcttaaa
     9301 agaatcattg ttggatcttc aaaaacagtt tgataaatta gatatagaaa aagaaaaagt
     9361 aggagaaaat aatttgagta tatcgtctaa tcttaccttt ttacttggag atccggtatc
     9421 tacaataaaa aagataatac aaacagacaa ggaaattgaa gcaatttttg ttaacagaga
     9481 ttatactcct tacagtatag aaagagatcg gtcaataaaa gaaatttgca gatcttttaa
     9541 tattgacttt attgattgtc cagatattct tattaatgaa ccagaagaga ttctgaattc
     9601 aactaatgaa ccatttaaag ttttttctca ttattataac aaggcaattg aaaaaccatt
     9661 aagaaaagaa aattcttttg atataaaaaa atacaaaatt aatactatta gttttgataa
     9721 tatcaacaac agcaaaaaat taaaaaattt taatagcatt gaaagaatta ataatcttga
     9781 ggaattcttt tctcaaatta taaatgaaca atctccgggt attaataata ctacttcctt
     9841 gtcatcacca acccaattat tgttgattgg aacaagaaaa aaatatcgag aattaatatc
     9901 agcattaaaa tataaattta acaaagataa taaacaaaaa gaatatatta atttattaga
     9961 caatacagca agccatcttt cgccttatct taaatttgga atatgttcca ttagggaagt
    10021 atattctata atccaaaagg aattaggttt caatcaccgt ttgctaagac aactatattg
    10081 gagagatttt tatgtctata taggatttca ctttcctttt gtatttacta gtccttttca
    10141 aggaagatat agaagaaaaa ataaaacaaa tgttattcaa tggaaaaatg acccacatga
    10201 atttgaaata tggtgtaatg gaaaaacagg ttttccaatt gtagatgcag gaatgagaga
    10261 actaaatgaa agtggctata tgcataatag agtaagacta atcactgctt cttttttggt
    10321 aaaagattta catattgatt ggcgatatgg agaaagatat tttgcattaa aacttgttga
    10381 ttatgatccc tctattaata atggtaattg gcaatgggtg gcttcaactg gatgtgatgc
    10441 tcaacccttt ttcagaatct ttaatccatg gttacaacaa aaaaagtttg atccaaattg
    10501 taattacata aaaaaatgga ttccagaact taaagacact cctataaatt taatacatga
    10561 atggtataat acagttaatt cgacaatcga caaatctact aaactctctc aaattgaaaa
    10621 ttatcctaga ccaatagtgg aacaccaaaa agaaattatg gttacaagag agttatacaa
    10681 tatttgatta attagcaata ataaattaaa acctattttt gcttatgctg ttttgcaagg
    10741 gctacaatat tttatgtctg agtataacta tttataattt caacacactt ctctcatttc
    10801 tttatttttt atctcgagat ttgagacgtg tgaaccaaca tctatacaat tttttatatt
    10861 cgccgagggt gggactcgaa cctcgaaccg gtagttcggg gtttgtcttt tctatgtaat
    10921 taataaaata ttctattggg ataaattaaa aaaaataaat gaaaaagata attttcaaat
    10981 cttagaatgt tgaaaaatta aatttattgt gtagtataaa tctataggct tagattggat
    11041 aaggatagaa aaaagattgg atcatattca actcagacaa aattgttttt tccaccaatt
    11101 caaaaatcta ttggcctagg cataattaca ggtgcagcag ataatgatcc atcatctatc
    11161 acaacttatt ctcaatcagg ggcaaaattt ggatttggtc ttctttggct agtaatattt
    11221 ctatatccgt tcattacagt aatggtcaac atttgcgcaa gaattggcat agtaacagga
    11281 aatggcttaa catcaattat atcaaaaata ttcaaaaaaa gttgtattga gatctgttat
    11341 ctatttaacg atctcaaagg cacccatcat gccaaggtcc tcatggaaca ttatgtgaca
    11401 gtgatagaca gattttccca caaaatcttc aaatggaatc cgaataacta cttcaccgtg
    11461 acctggaatc aacacagtat cttgtaatcc gtgagcgtcg taaggtttcc cattgactgt
    11521 tattacctga aaatcgttta catgaatgtg aaaaggatgc tcatcctcgt cggtatttat
    11581 tagcctccat tgctctacat ctccaagctt cactttttga tcgattctgt tatgatcaaa
    11641 tgtcttgtta tttatcgtga accgttgctc ctcctcgttt gatgaaaaag taaggactcg
    11701 attatttgtg atattcataa ggttcaggtc ttccttcgaa gttaaactcg atggaatagt
    11761 ttttacaggc tctttttggt taccttgtac gtttacagtc gcaattgtgg tctccgggta
    11821 tggatagtag tcaagtgctt ttaaaggaag agatccgttg cccgtcggag tcactaatac
    11881 atcaaaccgc tttccagaag gaagcacaag attatcagta tcccatacct tccaaaccgg
    11941 atatccatct tctgctacaa catggaattt gtggccaggc agtccaactt tgtagaaggt
    12001 ctcagatcca atgtttgcca aacgccatag ctgagtttct cctgatgtaa tgttgacatc
    12061 tggattgatt tctccgttga cagttcgata gataggcgca gttggatcac tattgactgg
    12121 aaaatctctc attgcaaagg tctgtttttt gatatcctgt aacggcatcg gcaataactt
    12181 ctctatccct tcaatcacta tcatgccaga taaaccggcc gaaacttgtc cataagcaag
    12241 agtgtgcata tgtgaatgat accagtacag acctggagcg tgatcaatag gaatgtcaac
    12301 aatatattgt tgtgtctctc cgggagcaac ttgaagaaaa acattatccg aattatttga
    12361 aggagacaca tgaagtccgt gataatggat attggtgggc tcatggagat tatttatcaa
    12421 attaagttca attctatcac ctggatatac atgcaaagtc ggtcctccaa gggaaccatt
    12481 atagaccata gaagtgattg actggttgtc aacattacct atatggtatg cagcagtgat
    12541 tgttgtttgc aaaacgccgt ctttactaaa tacatcttgg gattttgaaa agttcattcc
    12601 atccaaaggc gaaggttcag ctttggcaaa tgcgttatat tctaaaggtt caactgcaaa
    12661 catcatcaac ataaatatcg cgaaggaaaa agagtagatc ataccaccgg tattcagata
    12721 ggaccctatt ttgtggacca tttaagaaaa ttccaccaag tgttttgatc ttcctaaaca
    12781 attactttta tgttcaattc ctaaattgtt ctgtttatga ataaaataaa tttttttgtt
    12841 ctcactcatt tattgtaact aaatgtagtt gtgatggtag tatattaaca cattttccac
    12901 ttaaacacat ctaccaaaat tcaatccgtt tttaatattc aatcttattt tgttatagat
    12961 caatgatatc atatttttcc aagtagtaaa ttttcttctt agcaatatat aaattctgta
    13021 ttgaatatga attggatgtc tgacgaaaaa aatagaaaat caaacgatat gagaaaacca
    13081 attcgttttt tataattgga ttataaaata aactaattct cggattggga tttgaaccca
    13141 ttccttgcat tcacggatta atactaaaaa gtattatgaa ttttgtgttg gttaaaaatt
    13201 aatcaataat ctcttttgta ttcttttgat tcatctttgg ttatcttaaa ataaacagta
    13261 tcaccatcaa atcgttctat tagattttta ggaaagtaga atctgtctgt gcctacagtt
    13321 ccttttttcg ttattacatg ttctcctaaa acctcttgaa cttcaccaaa atcagtatca
    13381 tctcccagcc ctcttgcttc tttttttata atgtcttgta tattagagtc atttagagga
    13441 atatcatttg acatattctc tatggttcaa cattctatat aatgactaca attaaagtaa
    13501 aagattgttt tttaaataat ataaataaat gtctattgtt catttaaaat tttgaaaaca
    13561 agttttaatt aaaaatgatt tttataactt tggaaatttt tattagattt gtataatcac
    13621 aacattaata ataattttat tcctactgct gttgtgattc aatcatagca aacagaaaat
    13681 ttctttcgta ttctttcttt gattttatag aatttcagcc actaaacaaa acttttatac
    13741 actaatctac tccggccaga tttataaaaa tctacaaatt caacgataac tatctacaat
    13801 tttgtaataa atatatcagt aataatattg atgataaaga attgggatac agattaagga
    13861 ttttatttta acttgagatt tttattcata attataaaat tgtcatagct taaacaaaca
    13921 agcaacccag caactatact gggcttatac acacatatac atacttgtta ctactgctat
    13981 tgttttgttg tccagactat gataacaatt ttccatttgc tgagagattc cttattcttt
    14041 caagagatgg taacttcgat gataatgtaa attgtatata tcaatacgtg taaacctatg
    14101 ttgagagttc aatcaatgat ctattgttat tgttatacaa ataaaatatc aatcaatcaa
    14161 tattattctt cttattatta ttctttattt gataaattta taacttgtaa taattgatct
    14221 ttaaatttat tatcgtttaa aaattttacc ttaaaacagt cctcacagat ccagattcgt
    14281 tgatgaagac tcggagtttt atcttgttgt tgttgaagtt gtatgattgc gagtttagtg
    14341 gccctattcc tacacctatt aaattcattt atatagtttt gacatctgaa ttttcctaac
    14401 ctaactgcta ttctccctat atacacccta ttattatgtt cttctttgtc agatttctgc
    14461 atttcttaat attattattc aatattatgc tgaaaattgt ttcataattc ttaattttca
    14521 actcatatac tagcatatag ttctaattac tcaataaaaa acactagaga tatacaagat
    14581 ttatgactag tacattttaa ggggttgtat tctttaacaa tcttatgtga aataaaattt
    14641 tagtcattct gtacgactgg tggaatgatg atgataacaa tattagttcg catgaattag
    14701 aatgtaaaga agtagaaaat tatgataaag ttatggaatc acatattaat tatctaacta
    14761 tattctataa tcgtgcttta gtgtataagc aaaggctgaa gaagatttat ttagcaaaga
    14821 taaaaggaca ctcctagtaa agatctagga tatcactaga gtaatagaat tataaaatta
    14881 tatatataat ctaattctgt tttcaattat tgttataatt atagtattca attagataga
    14941 tagaatctca aaactcttac tcttactcca gagtagagat agacaaaacc taaaataata
    15001 taattataga cacttataaa ctttgatcta gacaataaat aaaaataaat aaattaaata
    15061 aattatgata aaggcattat tttgtttatc ctttggataa tatgttcatt actttttata
    15121 taatcttcca agatactagt gcattcttcc acatctagat tagacatcat attaacagta
    15181 acccaatgag caaaatcatc tattgttctc atgtagagtg ctgatttttc tcctgctgtg
    15241 gctaatttct cg
//
LOCUS       DASVEV010000132         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_47435, whole genome shotgun sequence.
ACCESSION   DASVEV010000132 DASVEV010000000
VERSION     DASVEV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47435"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VF222_05785"
     CDS             complement(<1..625)
                     /locus_tag="VF222_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX7141971.1"
                     /translation="MTQHRQTGITIISVLMIIGGLILLFTGITPNFTLNANGLNVNVI
                     PDSLSGLLAFHAQAEVRDTDIGSSEFGTDEKKFGFDKDFVYTSLNNNNTQGTPTTTTP
                     PSAPPPTCEVTVDTITRLGNAPFGIAYDSVNEKIYVTNSNDNTVSVIDITTNTVIDTI
                     PIGIEPFDIAYDRDNQRMYVSNGGDSTVSVINTTTNTVDPTPITVGSA"
     gene            1038..2522
                     /locus_tag="VF222_05790"
     CDS             1038..2522
                     /locus_tag="VF222_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7141972.1"
                     /translation="MQIILIVSFIIITIIATDENQLMISTSDSFAAAVSDRNIMTTTT
                     STFLKTSLQSSSQHDSIINSSSWNESNSSSSLSQSNSSSNKIKMPQITDSNLTVEIVV
                     DGLNMPTTMAFLGKDDFLILQKNGSLVRVIDGVVSDKTRLDFPVAKGFYQGLLGIAIT
                     NQSISTKNNETYVFLYYTEVSTTNNNTNINENSTRDGNSSTHILGNMVYRYEFVDNKL
                     INPKLLLKLPANSIENHGGYITIGPDNNLYIVIGEVVSEFDETAIQTLTQNYVNSTIV
                     DGRAGILRTTLDGNPVLDDKGHGLIGNTFPLNLYYAYGIHNGFGMDFDPITGILWNTE
                     PGHWINDEINIVRPGFNSGYGIVQGLSKYTPAAPIALVNFNGSGIYNEPEFVWTQKVV
                     PTGLKFLTSEKLGTQYHNDIFVGAFLDGRLYHFDLNQNRTHLSLPSKLSSQVLQNWNS
                     TGFEDIVFGTGFGGISSLNTGPDGYLYVISVEQGKVYRIIPINN"
     gene            complement(2935..>3255)
                     /locus_tag="VF222_05795"
     CDS             complement(2935..>3255)
                     /locus_tag="VF222_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7141973.1"
                     /translation="ITNNTIELQKNFANTFQSVFSKFIDNTSKSYWNNNLYPQRYTDV
                     YNKTNQNVTDNTVNVTRRINDIALASAETFNKSLEIAQKYYNETAQNYLNFVNKIEKS
                     YHNH"
BASE COUNT         1115 a          445 c          535 g         1160 t
ORIGIN      
        1 gtgcagaacc tactgtaatt ggagtagggt caactgtgtt tgttgtagta ttaattacag
       61 agacagtact gtcaccacca ttactaacat acattctctg attgtcacgg tcgtatgcta
      121 tatcaaatgg ttcaattcct attggaattg tatctataac tgtgtttgta gtgatatcga
      181 ttacagagac agtattgtca ttagaattag taacataaat tttctcgttt actgagtcat
      241 atgctatacc aaatggtgca tttcctaatc gagttatcgt atcaactgtt acttcacatg
      301 ttggtggtgg agctgaaggt ggtgttgttg ttgttggtgt tccttgcgta ttattattat
      361 ttagactcgt gtaaacaaaa tctttatcaa aaccaaattt tttttcgtct gttccaaatt
      421 cactactgcc aatatctgta tctcttactt ccgcttgtgc atgaaatgct aatagaccac
      481 tcaaagagtc aggaattaca ttaacattta atccattggc attgagagta aaattaggag
      541 taattcccgt aaataagagt attaatcctc caattatcat aagaactgat attatagtaa
      601 ttccagtttg acgatgttga gtcacataac aacaaagttt tcgttgaatt taagtattac
      661 ttgactaata taattatatc aattcaaaat gtagaaagag tcttttctat cgttatagtc
      721 aattaaataa ctcttttata taagggggtc atcagaagag aaatcttcca tgtattaaga
      781 ctctatttac ttgtatttag cttctattat tagaaactta ttttaaaatt tatgtttgaa
      841 aattgagaaa tttttgtatt attgttacgt ccaaatttct ttgaacttta ttaatttatg
      901 attaattatt aattgttaaa tagtagcagt tattttgatt cttattgttg aatatacatt
      961 tcatatttat aaaaaatgag gtttagaata gattgaggca caaaatatcg aataggattc
     1021 ttagtagagg ttttgctttg cagataatat taatcgtttc attcattatt attacaataa
     1081 tagcaactga tgaaaatcaa ttaatgattt ctacgtcaga ttcatttgct gcagcagttt
     1141 cagatagaaa tattatgaca acaactacat ctactttctt aaaaacaagt ttgcaatcat
     1201 catcacaaca tgattctatt atcaatagct ctagttggaa tgagagcaat tctagttctt
     1261 ctctttccca gtcaaatagt agtagtaata agattaagat gcctcaaata acagactcca
     1321 atcttacagt agaaatagta gttgatggtt tgaatatgcc tacaacaatg gcttttcttg
     1381 gaaaagacga tttcttgatt ctacaaaaaa atggaagtct ggttagagta atagatggag
     1441 tggtatcaga taaaactcga ctagatttcc cagttgctaa aggattttat caaggattac
     1501 ttggaatagc cattactaat caaagcatct caacaaagaa taatgaaact tatgtatttt
     1561 tatattatac tgaagtatct actactaata ataatactaa tattaacgaa aactcaacta
     1621 gagatggcaa tagtagtaca catattctag gtaatatggt ttatcgttat gaatttgtgg
     1681 ataataagtt aataaatccc aagttacttt taaaattacc agcaaattca atagagaatc
     1741 atggtggata tattactatt ggacctgata ataacttgta catagtaata ggtgaagtag
     1801 tcagtgaatt tgatgaaacg gccattcaga ctttgactca aaactatgtt aatagtacta
     1861 ttgttgacgg cagggctgga atactaagaa caacactaga tggtaatcca gttttagatg
     1921 ataaaggtca tggattaata ggaaatacat ttcctttgaa tctatactat gcatatggta
     1981 tacacaatgg gtttggaatg gattttgacc caattacagg catattatgg aatacagaac
     2041 ctggtcattg gattaatgat gaaataaaca tagttagacc tggtttcaac agtggatatg
     2101 gaatagtaca aggactaagt aaatatactc cagctgctcc tattgcgctt gtgaatttca
     2161 atggaagtgg aatttataat gagcccgaat ttgtatggac acaaaaggta gtacccacag
     2221 gtctcaaatt tcttacatca gaaaagcttg gcacacaata ccacaatgat atatttgtag
     2281 gggcattttt agatggaaga ttgtatcatt tcgacctcaa tcaaaatcga actcatttat
     2341 cgttaccttc aaaattgagt tcacaagtat tacagaattg gaattctaca ggttttgaag
     2401 atatagtctt tggaacagga tttggaggta taagcagttt gaatacagga ccagatggat
     2461 acctatacgt gatttcagtc gaacaaggta aagtctatag aataatacct atcaataact
     2521 aatttttttt tagctcgata tatgtcatat atattgatta agaatcgatt gtaatattca
     2581 ctttaaatta taccaatagc ggttcaaaaa ggggagtcta ttattgtaga tatactacta
     2641 acatgtattt agcttttatt attatcaaat tatctaaaaa ttgatttaac aattaatgaa
     2701 tttttattaa tatattaggg atatagttgg gctatcaaag taacaaaagg aagaggttca
     2761 ttgaggctca tgtaagacac atgtcctcta gtttggtttt tcagtccaaa gaactccaaa
     2821 cagaacctat tacaaaatag tagacaacat tatttaaata acttattata aattgttatt
     2881 tactttcttt actctttata atcaaatcaa atcaaaacaa aactaaaaaa aaaattagtg
     2941 gttgtgataa gatttttcta tcttattcac aaagtttagg tagttttgag ctgtttcatt
     3001 atagtatttt tgtgcaattt caagtgattt gttaaaggtt tctgcagatg ctaatgcaat
     3061 atcatttatt cgtcttgtaa cgtttacagt attgtcagtc acattttgat tagtcttatt
     3121 atatacatct gtgtatcgtt gtggatagag attattgttc cagtaagatt tggatgtatt
     3181 atctatgaat tttgaaaaaa ctgattgaaa agtattagca aaattctttt gtaattctat
     3241 agtgttgttt gtaat
//
LOCUS       DASVEV010000133        19682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_47776, whole genome shotgun sequence.
ACCESSION   DASVEV010000133 DASVEV010000000
VERSION     DASVEV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 19682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19682
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47776"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF222_05800"
     CDS             complement(<1..547)
                     /locus_tag="VF222_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX7141974.1"
                     /translation="MTSIIKAFAIDIDGTLTENGGGIIHLPAIQDLRTFEKLGYKIIY
                     VTGRSSVEAYMLSVFGGTTKIAVGENGGVVTISPKEHILLGNKEKCIDGYRILTKKLE
                     NVKIKNVFDRMTEVVLERSFDIELGKKILKENNLEVALSDSKYSFHINSRGINKATGL
                     AKALDILKIKPSETVTIGDSET"
     gene            complement(608..2044)
                     /gene="purF"
                     /locus_tag="VF222_05805"
     CDS             complement(608..2044)
                     /gene="purF"
                     /locus_tag="VF222_05805"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7141975.1"
                     /translation="MVTENCGVVGVFSLDQHNVIPILIDSLRALQHRGQESWGIAVPN
                     KKPFRQLGLVSTYADNFKNIIKNYKSNAGIGHVRYSTFGKSTIGNAQPLKVKDLCIAH
                     NGTIANVEELANMVGGCTFTPQTMSDTFIAAKRLVEHLSTRMNVKDSLKILKQEMIGS
                     FCFTFMTDNGSVYAARDTKGFRPLVLGFHKETNTYIIASESCAFSAVGAYLIRDVEPG
                     ELIKLSMNGLVTETFSSDKPHAHCAFEYTYFAHPVSIMEGVNIYAARKRMGEFLGKMF
                     PIPSADLVIPVPDSARPAALGYAQETGLPFEEGLVKDRHINKGTLRSFIEPFQSDRIE
                     INKGILPIKDIIDGKNVVIIDDSIIRGTSSASIIQTLREAGANKIYMIVTYPPVRFPC
                     YAGIDFPSMEELLANQKFLNNGQKLELNKKVSKLIGADKVFYNDTTNLAKAIGIPEEE
                     LCFTCSTGNYSSLGITPKFRTREEVKGE"
     gene            complement(2136..4364)
                     /gene="purL"
                     /locus_tag="VF222_05810"
     CDS             complement(2136..4364)
                     /gene="purL"
                     /locus_tag="VF222_05810"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX7141976.1"
                     /translation="MEILTKSELNYLNKELKRKPNSLEKEIVGAQWSEHCSYKSSKKY
                     ITLLPKKGERIIKGPGYDAGIIDIGNGDVITIHIESHNHPSAVEPYGGAATGVGGIIR
                     DIISMGTMPIAILNALRFGHINSLFSSNDIKPARGKNNESDSHSKWLLKNVVRGIADY
                     GNCIGIPTVGGEIEFDESFTNYCLVDVAAIGFGKKDNLIKNIIQEDDVLILAGSLTGR
                     DGIHGASFASKELAEENRSAVQIPDPFMEKLLIEVTREAIEKDILKAVKDLGGGGLSC
                     CLSETSANLNKGFDIDLCKVPVKYLDMEPQEIMISESQERMLYIISKNKLESFAKILK
                     KYDVPFSIIGKVKNHKNLVINKNGKTIAKMPSHLIAYAPLLNRKSKKPKYIDDLRFKK
                     TSLKVPNDLNKTILNMLANPTIASKNWVFQQFDHEIGLRTIVKPGITDSAVLKLNNDR
                     FLAAKLDGNSKLCYLDPYQGTLSCLSEARANIISSGGKPIGIVDHMQFGNPENPEIFW
                     TFQKSIEAIIDFSSFNKIPIVGGKVSFYNETKNGPIKPSPVMGMLGLIESRNCISKTS
                     PSPDDTLYIIGTTSDDMGGSEYYQYLNGLKGGSVPKLELGIDLLNSDVILNIIKKNLI
                     TCSHDCSKGGLSIALAEMAIASNCGINIDLDNVPNKCKRLDFLLFAETPSRFIIGTKD
                     EEKLETELSKRKNLTYEKIGKVTAFRANITFASKKKNIINLEMEIAKKYFENLSYIME
                     NY"
     gene            complement(4390..5082)
                     /gene="purQ"
                     /locus_tag="VF222_05815"
     CDS             complement(4390..5082)
                     /gene="purQ"
                     /locus_tag="VF222_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX7141977.1"
                     /translation="MLKIGIPIFPGSNCDRDVYHVLTNLYNVKTDMIWHKKDKIENYN
                     AIIIPGGFSFGDRLRAGIIAAHSPIIKEVKRLGNDGVPILGICNGFQILVECELLPGS
                     LIKNDCLKFTCKWIDLEVRNIDTPFTSTFKKNELFKIPIANGEGRFILNEEQLKEVKK
                     KNQIVFSYHNEDPNGSIESIAGISNDSGTIVGMMPHPERTCQVESTSFGLTNTAMKIF
                     DSLITYIKKISS"
     gene            5235..5651
                     /locus_tag="VF222_05820"
     CDS             5235..5651
                     /locus_tag="VF222_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7141978.1"
                     /translation="MIKDVLTISENDTALKAAQLMTEKGVSSLIVLSDDQPIGIVTER
                     DFIKKICLKELKLSNVKVGDMMSRIRTSASPDIPIDVAVQRMVNNRIRRLPIFENGKV
                     VGIITVTDLAKHLRTILLLNSTLSASHLSQSTLESY"
     gene            complement(5710..5979)
                     /gene="purS"
                     /locus_tag="VF222_05825"
     CDS             complement(5710..5979)
                     /gene="purS"
                     /locus_tag="VF222_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX7141979.1"
                     /translation="MSNKEFNVIVTIENKSFINDPEGETIYRDLILKNKYNSIKSVRT
                     AKVLKLIVDAKNPREATMRVRKMCEDLRIFNPIVSNCQVNITSDK"
     gene            6072..6146
                     /locus_tag="VF222_05830"
     tRNA            6072..6146
                     /locus_tag="VF222_05830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6107..6109,aa:Thr,seq:tgt)
     gene            complement(6173..6907)
                     /locus_tag="VF222_05835"
     CDS             complement(6173..6907)
                     /locus_tag="VF222_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7141980.1"
                     /translation="MFTIYQILIIAYTTGILWLRRNPLSLIFTAITPFSLLFVLFIIS
                     DGQYTQFAISGSLVMALVGYGLALGQDITYYKTEYKIQDMFVASPVSPLTYMTGLALS
                     EILFGFPALLILLFLIFYIGISWFYVPLLIFIILIVWISTSSMGFFLSSHMLHMKNAT
                     QIISFVNIFLTILPPVFYSIYKLPDTLQYVSYIVPTTHASLMVQYAMGFDTPANWSVL
                     IGISVLLVYAIGFIALAKAHAIWRDT"
     gene            7040..7915
                     /locus_tag="VF222_05840"
     CDS             7040..7915
                     /locus_tag="VF222_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7141981.1"
                     /translation="MKLLHQGKVKDVYYNGNNNENKNTLLFVFSDRISAFDVKMKQEV
                     PKKGEILCKFAKFWFDNLNVEHHMIKTIDANKMLVEHLSMIPIECVVRGYFYGSLVDR
                     YNNNKIDWKNSQDFQILSHNFDPVIASKLSEPLFDPTTKSLDHDIPITKNEVISSNIL
                     SKNDFDFLKNKSIELYLKMSKIINLAGFLLADVKFEFGFNRNGVIVLGDSLGPDEYRI
                     WKNDYYQKGSIQESYDKQILRDWLTTMGFKREIDESKSYNKKPAPPHLPSSLIEKISK
                     RYILAYEMITGDKIR"
     gene            complement(7930..8364)
                     /locus_tag="VF222_05845"
     CDS             complement(7930..8364)
                     /locus_tag="VF222_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HEX7141982.1"
                     /translation="MITEEIKFYGHPNVLATHPRTIEITKDNYLTKEGNCIVGINANK
                     ACSDFNPKLRNLIMKDNVPIKFKITVSNQSFEINGFGNSKLTLSSPHDIVIRTSNFIC
                     TRTASIKCDKASVHVPREIILLLQDAKTHGSLLISVNDYESA"
     gene            8424..9167
                     /gene="psmA"
                     /locus_tag="VF222_05850"
     CDS             8424..9167
                     /gene="psmA"
                     /locus_tag="VF222_05850"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HEX7141983.1"
                     /translation="MLPAAAGYDRAITVFSPDGRLYQVEYAIETVRRGTLALGIKCKD
                     GVLLAVEEKTRKLQISDVTQKIFQIDDHIGVAAAGYIPDARAQVDHARFFAQSNKLIY
                     DEPVDVEIVAKNIADMSQQLTQYAGVRPFGVALIIAGVDTTGGELFLTDPSGTFIGYD
                     AIAIGQGSDQVNEFLEKYYTQNTSLEEAGKLALESINMVSEEKTGISHIKMSLIENES
                     KIMRKVAEEEIDKFAKIAKENPTNPKEKP"
     gene            9212..9874
                     /locus_tag="VF222_05855"
     CDS             9212..9874
                     /locus_tag="VF222_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEX7141984.1"
                     /translation="MTLQMGTKAPNLHVSEWVQGLPTNIDKNIGKVVLVEVFQVNCPG
                     CFMYALPNAIEIYKNNNREDVVVLGLATAFEDYDKNTLDNLKLLLSSGKVIGETFNAL
                     NQYRQLVDGDKLNYKIPFPVAMDMLRTNTQDFSDNKVIDFIESTIDNYNSYSEPDKKV
                     LFERVKQYLKNKKYSALTFDEYKLQGTPSAILIDKKGNLRNVFFGVNNALNGLIKQLV
                     NE"
     gene            9871..10788
                     /locus_tag="VF222_05860"
     CDS             9871..10788
                     /locus_tag="VF222_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7141985.1"
                     /translation="MTIKDRISVAIPDSSLSDEKGLREKTLKIGKFARTFSIFGVEKI
                     VIYKDPTETRTKSDMFLLHLILEFLNTPQYLRKSIYPVNSDLSYIGLLPPIRAPQHTN
                     KVRIKDVKVGEIRVGVLHDKNSMLKKMDRKTFTKNDKSIINFNKERFDFYIDVGLDYP
                     IPFLGEGRDGEKVVVKFVNSYPHLKVIKASGRDLEKLYFGYSIVKVNSLEDFLKKLDE
                     KTFVIFTSKNGRIFKSVEFQFKQSINNFENLLIVFGSPSKGIKEIYTNYHTTKNSMYL
                     NMFPDQKTQTIRLEEAILGTLTLFNHFLL"
     gene            10875..11888
                     /locus_tag="VF222_05865"
     CDS             10875..11888
                     /locus_tag="VF222_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7141986.1"
                     /translation="MGHRKYSSPRRGSLAFYPRVRAKSEEARLRNWPEHSDNASLLGF
                     AGFKAGQIQVMTIDDKEKTPNFGKQLDNSSTVIATPPINIIGIRCYKKDLYGEKAFID
                     AYSKDLPKGLERKFKTKYHEKSFETLESSLDSIFSFFAIASVFPKKANLSQKKPFVFE
                     IGVSGKDNKTKYEYLKGILGKEIKISDVFQNGQYIDVSGITRGKGVEGPITRFGVKRK
                     QHKSRKSVRALGTLGPISPAVVMYSVPRQGQRGFHQRTEYNKRILLVSSNGSSQTTLV
                     ASKPTINPPGGFKHFGLVTTDYIVVRGSIPGVPKRLVKMRQPIRNTTKKVIEPKVIEV
                     IAK"
     gene            11885..12691
                     /gene="rplD"
                     /locus_tag="VF222_05870"
     CDS             11885..12691
                     /gene="rplD"
                     /locus_tag="VF222_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7141987.1"
                     /translation="MSIINIIEMDGSNKSTVTLPGVFQTPYRPEVIQKVYNHLNSYTF
                     QRQGRYPAAGQMVSAESRNTGLGIARIARARGEGFPRAGQAAGVASVRHGRLAHPPVS
                     WKNIYKKVNKKEKLLALCSAIAATTNTELIQRRGHKIKNDLQLPIVVSNEIESIVKSK
                     DLEKILLKLGLEEDLKRTFIRRNKSVHKNGTNRRSALSVLIIVSNAEKIGRLNNSLPG
                     ITVKSVKSISVLDLAPGSKPARLTIYSENAIKELNNLKPTSNIISEMIAQ"
     gene            12688..13014
                     /locus_tag="VF222_05875"
     CDS             12688..13014
                     /locus_tag="VF222_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7141988.1"
                     /translation="MKKNLFTLKSTRSSKQKKKISQLQKDRPLIIEPYITEKSFNLIE
                     KEKKLTFIVDEQADKKSIKQELHDLYEAEIIEVNTARTIDGKKAFAKFKDLDSARDLA
                     TKLGLV"
     gene            13086..13493
                     /locus_tag="VF222_05880"
     CDS             13086..13493
                     /locus_tag="VF222_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7141989.1"
                     /translation="MVREFKFRGYNQDQLKSISIESLLPLLNARQRRSLNKRVGKYMN
                     DQKRTLREEIKLARDGKLKNKLKTHVRDMIILPDMIGLSISVHNGKEFFDVIIKSEMV
                     GHYLGEYAITNKRVQHGSPGVGSSRSSLYVPLK"
     gene            13501..13977
                     /locus_tag="VF222_05885"
     CDS             13501..13977
                     /locus_tag="VF222_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7141990.1"
                     /translation="MPNYSYAYQNFDKSRQVRSALREKDISHKHAREIALAIKNLSIE
                     KARDYLDAVLRKEIAVPFRRYNNEVAHRSNIRDGFSAGRFPSKATHEFLKLLDNLESN
                     AEYKGMDLDRLKIINIVVHKGTRLKRFTPRAMGRASPKFDTLVHVEMVAQEGVITE"
     gene            13974..14798
                     /locus_tag="VF222_05890"
     CDS             13974..14798
                     /locus_tag="VF222_05890"
                     /inference="COORDINATES: protein motif:HMM:NF003219.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7141991.1"
                     /translation="MNAAKNVMSSNYKNMELDEYLKETLRETGYGGVDIQKTPIGTRI
                     TLYVTRPGLVIGRKGSGIKDLTSKLEQRFGLNNPQISVMEIEVPELNPQIMANRIAQL
                     VERGTAFRRASMWTMNTVMGAGALGVEISIAGKLRSERAHFEKHSSGVVPKSGNAADK
                     IVKLGITDVLTKMGLMGIQLRIALKNEVPPDFELIESKQENLQNSDPQLDQGTDNEAN
                     KNTIQENTTRKNSSERNNQNIKRQQQKQIPKSKDIKDNSMNQNKVKEKGSVPTNTK"
     gene            14795..15013
                     /gene="rpmC"
                     /locus_tag="VF222_05895"
     CDS             14795..15013
                     /gene="rpmC"
                     /locus_tag="VF222_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7141992.1"
                     /translation="MSRIKLTTLREMNDQDLEDKLLELKADLAKLRSEGAKGTLKKES
                     SSIRWARRDIARILTLINERKIKKRVKI"
     gene            15010..15282
                     /locus_tag="VF222_05900"
     CDS             15010..15282
                     /locus_tag="VF222_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HEX7141993.1"
                     /translation="MRSIENILYHEWIGLKIKLLECSQSSLTNLSGQIIFETKNMLIV
                     RTLNGIKKIPKKIILKCILYLPSTACFIRGNQLIGRPEDRILKIKR"
     gene            15292..15627
                     /locus_tag="VF222_05905"
     CDS             15292..15627
                     /locus_tag="VF222_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7141994.1"
                     /translation="MVRNIGIMVSLPKKTCEDQHCPFHGSLSVRGRLYTGIVESNKSP
                     KMIVVSRQYNRLVKKYKRYERSKSKVHAFLPDCIDVNEGQKVKIAECRPLSKTVSFVV
                     VEGDNKDGI"
     gene            15617..16051
                     /locus_tag="VF222_05910"
     CDS             15617..16051
                     /locus_tag="VF222_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7141995.1"
                     /translation="MASKSRAVSAKGASEFRPYITRALPVTAELVCADNTGAKVLRIV
                     QVSKYHGRHSRMPSGAVGDLVTVTVKKGPAQLRKQIFGAVIIRQKYPIRRLSGLRLTF
                     EDNAAVLVTPEGEIKGTDIKGPVAAEAAEKWPRIANLASSIV"
     gene            16069..16557
                     /gene="rplX"
                     /locus_tag="VF222_05915"
     CDS             16069..16557
                     /gene="rplX"
                     /locus_tag="VF222_05915"
                     /inference="COORDINATES: protein motif:HMM:NF028216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7141996.1"
                     /translation="MTNKNTTLNIKKHILDKHIRSPLSENLRKEYNKRNARVITGDTV
                     KVLRGEYKNVEGKVEKVKTRRSTLFIEGIQREASKGGKVKVQIHSSNVIITSFNLHDK
                     NRSNVIRKVKTANLEKKGINIKPNITKTRIKRNLNVKKVLKPQQKRITDSNQSQDNKG
                     EK"
     gene            16617..17345
                     /locus_tag="VF222_05920"
     CDS             16617..17345
                     /locus_tag="VF222_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HEX7141997.1"
                     /translation="MANKGGRTTVKRQLAPVFWDINRKQGRFIVRTYPGTHAKKYAYS
                     IGVILRDVLKVTKNMHEAKKIVNAGKVKVDNVIRYDVNFAVGLMDTIELVSSQQKVYR
                     LVPSNSLLLFPIEISDNEKTLKLVKIKTKNIIKGGKIQYGFHDGKSVISENVYSVGDT
                     CLIKVPELEIIETLKLQSDCLAILTRGDNAGSIGKIQEIKEGTFSLPKRVIISVDSRL
                     LEIPIDMIMVISERDNNNPKIKVN"
     gene            17348..17875
                     /locus_tag="VF222_05925"
     CDS             17348..17875
                     /locus_tag="VF222_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7141998.1"
                     /translation="MSENNNDSNEKMKRIDIEKIVINIGVGKSGDPFERAKMALEELT
                     GQKPSPRGAKKSVRDFGIHKGEPIGAMVTLRRQIALDILKRIFLAKSNSFKRTSIDNN
                     GNLSIGIKEHIDIPGIKYNPDIGIFGMDVCISLKRPGYRISKKKHPDKIGKNHRITKE
                     DSINFFQKNFGVEAI"
     gene            17875..18054
                     /locus_tag="VF222_05930"
     CDS             17875..18054
                     /locus_tag="VF222_05930"
                     /inference="COORDINATES: protein motif:HMM:NF004424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX7141999.1"
                     /translation="MPKPREYSETGRKKVPHGKGTRWCKRCGAFNGLIQKYDIMLCRQ
                     CFRDVAINLGFKKYE"
     gene            18153..18545
                     /locus_tag="VF222_05935"
     CDS             18153..18545
                     /locus_tag="VF222_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7142000.1"
                     /translation="MPSLNIVANLFNTLYNNEIRRKKECLVIPTSKFASEILRVMQKY
                     RYIGEFELIDDNRMGKFRIQLLAKINKCGIITPRYGVKKNEFLNWERQFLPAYNLGIL
                     LVSTSKGIMSHTDAQSQGLGGVLIGYVY"
     gene            18535..19089
                     /locus_tag="VF222_05940"
     CDS             18535..19089
                     /locus_tag="VF222_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7142001.1"
                     /translation="MSTSKEVNQSGEVIIPESVKVTKEKNTIIVEGKNGKISKDFTKV
                     PITLVFENNKIIIHPPGQRRKDLALINTVKSIISSMIKGAETGFTYKLKIIFSHFPIS
                     VKVKDKKVIVENYFGERSSRISKIVGLTQVVVTGEDVIVKGPSLEDVSQTATNIESST
                     KIKNKDQRVFLDGVYLYAKENGMN"
     gene            19256..19359
                     /locus_tag="VF222_05945"
     tRNA            join(19256..19283,19314..19359)
                     /locus_tag="VF222_05945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19320..19322,aa:Asn,seq:gtt)
     gene            19591..>19682
                     /locus_tag="VF222_05950"
     CDS             19591..>19682
                     /locus_tag="VF222_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7142002.1"
                     /translation="MKAVILAGGLGKRLKPLTNDRPKPMIEVLNV"
BASE COUNT         7138 a         2970 c         3321 g         6253 t
ORIGIN      
        1 cagtttcact atctccaatt gttactgttt cagaaggttt aattttcaaa atatctaatg
       61 ctttggcaag acctgtagcc ttattgattc ctctactatt aatatgaaaa gaatatttgc
      121 tatcactcag agctacctca agattatttt ctttaaggat tttctttcct aattcaatat
      181 caaaacttct ctctaataca acctcagtca tacgatcaaa aacatttttt attttaacat
      241 tttccaattt ttttgttaaa attctatacc catcaataca tttttctttg tttccaagaa
      301 gaatatgctc tttcggactg atagtaacca ctccaccatt ttctccaaca gctatttttg
      361 tagtccctcc aaatacagac aacatataag cttcgactga agaccgtcca gtaacgtaga
      421 taattttata tcctaatttt tcaaaggttc taagatcttg aatagctgga aggtgtataa
      481 ttcctccacc attctcagtc aaagttccat caatatcaat tgcaaaagct ttaattattg
      541 aggtcatagg aaataatatt gagttaggaa tttaaccttc tcttttttaa aaatataata
      601 gtgtatttca ttctcctttt acttcttctc ttgttctaaa ctttggtgtt attcctagag
      661 aactataatt cccagttgaa catgtaaaac ataactcttc ttcaggtatc cctattgctt
      721 tagcaagatt tgttgtgtca ttataaaata ctttatctgc accaattaat ttactaactt
      781 ttttattaag ttctagtttt tgtccattat taagaaattt ttgatttgct aatagttcct
      841 ccatagaagg aaaatcaatt cctgcatagc aaggaaaacg cacaggagga tacgtgacta
      901 tcatataaat tttgttagct cctgcttctc tcaaggtttg aataatagag gctgagctag
      961 tccctctgat tatactgtcg tcaattatta caacattttt tccatcgata atgtctttta
     1021 taggaagaat ccctttattt atttcaattc ggtccgattg aaatggttca ataaaacttc
     1081 ttaatgtacc tttattgata tgcctatcct taactaagcc ttcttcaaag ggtagccctg
     1141 tttcttgtgc ataacctaat gcagcaggcc ttgcggaatc aggtactgga atgactaagt
     1201 ctgcagaagg aattgggaac atcttaccca aaaattcccc cattcttttt ctagctgcgt
     1261 aaatattcac accttccata atacttacag gatgagcaaa ataggtatat tcaaaagcac
     1321 aatgggcatg aggtttatct gaagaaaacg tttcagtcac taaaccattc atgcttagtt
     1381 taattaattc tcctggctcg acatctctta ttaaataggc tcctactgca gaaaaggcac
     1441 aggattcgga tgcaataata tatgtatttg tttctttatg gaatcccaaa acgaggggtc
     1501 taaagccttt tgtatctctt gccgcataaa cagatccatt atcggtcatg aaggtgaaac
     1561 aaaaggaacc tatcatctct tgctttaaaa tttttaaaga atcctttaca ttcattctag
     1621 tagaaagatg ctctacaagt cttttagctg ctataaacgt atcactcatt gtttgaggag
     1681 taaacgtaca acctccaacc atattcgcca attcctcaac atttgcaatt gttccattgt
     1741 gcgcaataca caaatcttta acttttaaag gttgggcatt ccctattgta ctcttgccaa
     1801 aagtagaata tctaacatga cctattccag cattagattt gtaattttta atgatattct
     1861 taaaattatc tgcataagtg gaaaccagac ctaattgtct aaatggtttc ttattgggaa
     1921 cagctatgcc ccatgattct tgacccctat gttgaagagc ccgtaaagaa tcgattagaa
     1981 tcggaatgac attatgttga tcaagagaaa aaacacctac aacaccacaa ttttctgtta
     2041 ccatttcaat acaccattac ttatccatta aaatatttta atcatatgtt tagatacgaa
     2101 atcttttaat caaaacaaat actcattgct ttagttcaat aattttccat aatatatgag
     2161 agattttcaa aatatttttt tgcgatttcc atttccagat ttataatgtt ttttttctta
     2221 gaagcaaatg taatgttagc tctaaaagcc gttacttttc ctattttttc atatgttaaa
     2281 tttttacgct tagaaagttc tgtttcaagt ttctcttcat cttttgttcc aataataaat
     2341 ctagatggag tttctgcaaa aagaagaaaa tctaatcttt tacatttatt aggaacgtta
     2401 tctaggtcta tattgattcc acaattagaa gcaattgcca tctcagccaa agctatagag
     2461 agaccaccct tcgagcaatc atgagaacaa gtaattaagt tttttttaat aatgtttaaa
     2521 attacatctg aattaagtaa atctatgcct aattccagtt ttggaacaga cccccctttt
     2581 aatccattta agtactggta atattcagaa ccacccatat catctgaagt tgttcctata
     2641 atgtataagg tgtcatctgg actaggactc gttttggaga tacaattcct actttctata
     2701 agtccaagca ttcccattac tggtgagggt ttaatgggac catttttagt ctcattataa
     2761 aaactaactt tgccaccaac aattggaatc ttattgaaac tgctaaaatc aataattgct
     2821 tctatacttt tttgaaaggt ccaaaaaatt tcaggatttt caggatttcc aaattgcata
     2881 tgatcaacta ttcctattgg ttttcctccc gaggaaatta tatttgctcg agcttcagaa
     2941 agacaggata acgtgccttg atatggatct aaataacata atttggaatt tccatctagt
     3001 tttgcagcca aaaatctatc attatttaat tttaggactg cagaatcagt aattcctggc
     3061 ttaacaatgg ttcgtagtcc gatttcatga tcaaattgtt gaaataccca attcttggaa
     3121 gcaatagttg gattagctag catattaaga atggttttat ttaggtcatt tggaaccttc
     3181 agtgaggttt tcttgaatct taaatcatca atatatttag gctttttact ttttcgattc
     3241 aacaatggag catatgctat caaatgtgaa ggcatttttg caatagtttt tccgttctta
     3301 tttattacta ggtttttatg atttttaact ttaccaatta tggagaaggg aacatcatat
     3361 tttttaagaa ttttagcaaa actttctaat ttatttttgc taataatata taacattctt
     3421 tcctgtgatt cagagatcat aatttcttga ggttccatat caagatattt tacaggaact
     3481 ttacacaaat caatatcaaa acctttattt aaattagcgg aagtttcgga aaggcagcaa
     3541 gacaatccac ctcctcctag atccttaaca gcttttagaa tatccttttc aatagcttct
     3601 ctggttactt caattaataa tttctccata aatggatctg gaatttgaac tgctgatcta
     3661 ttttcttctg ccaactcctt tgatgcaaac gatgcaccat gtatcccatc tctaccagtc
     3721 aaactgcctg ccagaatgag aacatcatct tcttggataa tatttttaat caaattatct
     3781 ttttttccaa atcctattgc tgccacatcc actaaacaat aattggtgaa agattcatca
     3841 aattctatct ctcctcctac tgtaggtatt cctatacaat ttccataatc agctatccct
     3901 cttactacat ttttcaataa ccatttagaa tgagaatctg attcattatt ttttcctctt
     3961 gcaggtttta tatcattaga tgaaaagagg gaattaatgt gtccaaatct taatgcattt
     4021 agtatagcaa ttggcatagt tcccatagaa ataatatcac gaattatacc tccaactcca
     4081 gtagcggcgc ctccataggg ttctacagct gatggatggt tatgactttc gatatgtatg
     4141 gtaattacgt caccattacc tatatcaata attccggcat cgtatccagg gccctttata
     4201 atcctttccc ctttctttgg tagtaaggtg atatattttt tagatgattt ataagaacaa
     4261 tgctcagacc actgcgctcc aacaatctcc ttttctaaag aatttggttt acgctttaat
     4321 tctttgttta aataatttaa ttcagattta gttaatatct ccaaatacta tctatgttca
     4381 ttagactatt tatgagctta ttttttttat atatgtaatt aatgaatcga aaattttcat
     4441 agccgtattt gtcaatccaa acgacgtcga ttctacttga caagttcttt caggatgggg
     4501 catcattcct acaatagtgc ctgaatcgtt actaatccca gcaatagatt cgatagaacc
     4561 attaggatcc tcattatggt aactaaaaac tatttgattt ttttttttaa cctcttttaa
     4621 ctgctcttca ttcaaaataa atctgccttc tccattagca ataggaattt taaataattc
     4681 attcttttta aatgtagaag taaaaggagt atcaatgttt ctaacttcca aatctatcca
     4741 tttacaagta aactttaggc aatcattctt aattagtgaa ccagggagta attcgcattc
     4801 aaccaaaatt tggaatccgt tacatattcc tagaataggg actccatcat tgcccagtct
     4861 tttcacttct ttgattatag gactatgagc agcgatgatt cctgctctta atctatcacc
     4921 aaatgaaaaa ccacctggaa tgattattgc attgtaattt tctatcttgt ctttcttatg
     4981 ccagatcatg tcagttttta cattgtataa atttgttaaa acatgataaa catctctatc
     5041 acaattacta cctggaaaaa ttggaatccc aatttttagc attcgaatat tctactaata
     5101 cctccaggga attaatattt ttggatctat tttagatatt tccaagaaaa aaatattctt
     5161 taaattgata gaattataag gtttttcatt ttattatata tttcattctc atgattgaat
     5221 tagtaagaga tataatgatc aaagatgtgc tcacaatatc tgaaaatgat actgccttaa
     5281 aagctgcaca attaatgact gaaaaagggg taagctcttt aattgtttta tctgatgatc
     5341 aacccattgg gatagttaca gaaagagatt ttatcaaaaa gatttgtcta aaagaattaa
     5401 aactttcaaa tgtaaaagta ggggacatga tgtctagaat tagaacttct gcatcccctg
     5461 atatcccaat cgatgttgca gttcaaagaa tggtcaataa taggattaga agattaccaa
     5521 ttttcgaaaa tgggaaagtt gtaggtataa tcactgtaac tgatttagcc aagcacctgc
     5581 gtactatatt acttttgaat agtacacttt cagcaagtca tttatctcag agtactctgg
     5641 aaagttattg aataataata tgttgtcaat tttttttaga tgtaatcttt tgaaagatct
     5701 taattaaaat tatttatcag acgtaatatt aacctgacaa ttacttacaa tgggattaaa
     5761 tattcttaaa tcctcgcaca tttttcttac tctcattgtt gcttccctag gatttttggc
     5821 atctacaatt aattttaata cttttgcagt tcttacagat ttaatactat tgtatttatt
     5881 ttttaaaatt aaatcacgat aaatagtttc tccttccggg tcatttataa aagatttatt
     5941 ttctatagta acaataacgt tgaactcttt atttgacaat tagatttcct gattattatt
     6001 gttacttaaa tacattttaa ttattggcaa agaattaatt atagtgttta taaaaaatcg
     6061 atgatattaa tggtcatgta gctcagcctg gccagagcga aggttttgta aaccttaggt
     6121 cgtgggtcca aatcccacca tgacctacga gttaaaatta atttagtctt catcaagtgt
     6181 ccctccagat tgcatgtgct tttgctaggg caataaaacc aatagcgtat actaacagta
     6241 ccgatatacc aatgaggacc gaccaatttg cgggggtatc aaatcccata gcatattgaa
     6301 ccatcaagga agcatgagtc gtaggaacaa tatatgaaac atattgaagt gtatctggga
     6361 gcttataaat tgaataaaaa acaggtggta atatggttaa aaaaatatta acaaaagata
     6421 taatttgagt cgcatttttc atatgtaaca tatgcgaaga taaaaaaaat cccatactag
     6481 aagtagagat ccacacaata agtattataa aaataagtaa tggaacgtaa aaccacgata
     6541 tacctatgta aaaaattaaa aataataaaa ttaatagtgc tggaaatcca aataaaattt
     6601 ccgatagtgc tagacctgtc atataggtta atggcgatac aggagatgct acaaacatgt
     6661 cctggatttt atattctgtt ttataatatg taatatcctg acccagtgct agtccataac
     6721 ccactaaagc cattaccaaa cttccgctta tagcaaattg tgtatattgt ccatcactga
     6781 tgataaataa aacaaatagt aatgaaaaag gagtaattgc agtaaaaatt agtgagagag
     6841 gatttcttct caaccataat ataccagttg tatatgctat aattagtatc tgataaattg
     6901 taaacatata agcattattt tttgaactaa ttttgttata ttttaaattg cggttctcgt
     6961 tataaaaaat catattagtt aatatttgtt ttttataatc gataattata aaaagatatt
     7021 ttttgttata ttaatactat tgaaattatt gcatcaagga aaagtcaaag atgtatatta
     7081 taatggtaat aataatgaaa ataagaatac tctgctattt gtcttctctg atagaatatc
     7141 tgcatttgat gttaaaatga agcaggaagt tcccaaaaaa ggggaaattt tatgtaaatt
     7201 tgcaaagttt tggtttgata atctcaacgt cgaacaccat atgataaaaa ctattgacgc
     7261 caacaaaatg ctagttgaac acctttctat gataccaatt gaatgtgtag tcagaggtta
     7321 tttttacggt agtctagttg atagatataa caacaataaa attgattgga aaaattctca
     7381 agactttcaa attttgtctc ataattttga tccagtaata gcttcaaagc tatctgaacc
     7441 attatttgat ccgactacta aatccctcga ccacgatatc ccaattacta aaaatgaagt
     7501 aatttcctct aatattttat caaagaatga ttttgatttt ttgaaaaata aatccataga
     7561 actttattta aaaatgagca aaattataaa cttggcgggg tttttacttg cagatgtaaa
     7621 atttgaattt ggttttaata ggaatggcgt aatagtattg ggagattctc ttggtccaga
     7681 tgaatataga atatggaaaa atgattatta tcaaaaagga tctattcaag aaagttacga
     7741 taaacaaatt ctaagggact ggctaactac tatggggttt aagagagaaa ttgatgaatc
     7801 taagtcttat aataaaaaac ctgcacctcc tcatttacct tcgagtttga tagaaaaaat
     7861 ttctaaaaga tatattcttg catatgaaat gataactggg gataaaatta gataatataa
     7921 gagatgtcat tatgcactct cataatcatt tacggaaatt agtaaggatc catgagtctt
     7981 agcatcttgt agtaacaata ttatttctct aggtacatga actgaagctt tatcacactt
     8041 tatcgaggca gttcttgtgc atatgaaatt actagttcta attacaatat catgagggct
     8101 gctcaacgtt agtttgctgt ttccgaatcc gtttatttca aatgattgat tgctaacagt
     8161 tattttgaat tttattggaa cattatcctt cataatcaag tttcgtaatt ttggattaaa
     8221 atcgctacaa gccttgttag cattgattcc cacaatacaa tttccttctt ttgttaaata
     8281 attgtctttt gttatctcaa ttgttcttgg atgtgtggca agaacatttg gatggccata
     8341 aaacttaatt tcttcagtta tcaatttgta agtgcgttaa taaggtaaat ttaaattaaa
     8401 cactggcaag tagtaatatc gaattgttac cagcagcagc aggttatgat agagctataa
     8461 cagtattttc tcctgatgga aggttatatc aggttgagta tgcaattgag acagtaagaa
     8521 gaggtactct tgctttagga ataaaatgta aagatggagt tcttttggca gttgaagaaa
     8581 aaacaaggaa attacaaatt tctgacgtaa ctcaaaaaat cttccaaatt gatgatcata
     8641 taggagtagc tgcagcaggt tatattcctg atgctagagc acaagtagat catgctagat
     8701 tttttgctca gagtaataaa ttaatttatg atgaaccagt tgatgtagaa attgtcgcca
     8761 aaaatattgc agatatgtct caacaattaa cacaatatgc aggagtgaga ccctttggtg
     8821 ttgcattaat cattgcagga gtggatacaa ctggaggcga attattttta actgatccaa
     8881 gtggcacatt tattggatat gatgcaattg ccataggtca aggaagtgac caggtaaatg
     8941 agtttttaga aaaatattac acacaaaata cctcattaga agaagcaggg aagttagctt
     9001 tagaatcaat taacatggta agtgaagaaa aaacaggaat ctctcatata aagatgtcct
     9061 tgatagaaaa tgaaagcaaa attatgagaa aagtagctga agaagagatc gacaagtttg
     9121 ccaaaatagc caaagaaaat cctacaaatc ctaaagaaaa accatagtaa atagatacgt
     9181 tttacgaatg tttatattta ttttggttta attgacctta caaatgggta caaaggctcc
     9241 aaacttacac gtttcggagt gggtacaagg attgcccacg aatattgata aaaatattgg
     9301 aaaagtcgtt cttgttgaag tattccaagt taattgtcca ggatgtttta tgtatgcact
     9361 tccaaatgca atagaaatat acaaaaataa taacagagaa gacgtagtag tattgggatt
     9421 ggctacggca tttgaagatt atgataaaaa tactctcgat aatttaaaat tattattatc
     9481 ctcgggaaaa gtaattggag aaacattcaa tgcattaaac cagtaccgtc aattagttga
     9541 tggtgataaa ctaaattata aaattccttt tcccgtagct atggatatgc tacgtacaaa
     9601 cacccaagat ttttccgata ataaggtcat tgattttatt gaatccacta ttgacaacta
     9661 taattcttat agtgaacctg ataagaaagt cctatttgag cgagtaaaac aatacttaaa
     9721 gaataaaaaa tattctgctt tgacttttga tgaatacaaa ctacagggta ctccttctgc
     9781 aatcttgatt gataagaaag gcaacttgcg gaatgttttt tttggagtta ataatgctct
     9841 caatggtttg ataaagcaat tagtgaatga atgacgatca aagatagaat ctccgtagcc
     9901 atccctgatt cttctttatc cgatgaaaaa gggttgagag aaaaaacttt aaaaattggc
     9961 aagtttgcga gaacattttc tatttttgga gtagaaaaaa ttgttattta caaagatcca
    10021 accgaaactc gaacaaaatc agatatgttt cttcttcact tgatccttga attcttgaat
    10081 acaccacagt atctaagaaa gtcaatttat ccggtgaata gtgacttgtc ttatataggt
    10141 ctgcttcctc ctattagagc tccccaacat acgaacaagg ttagaatcaa ggatgtcaag
    10201 gttggtgaaa taagagtagg agttctacac gataaaaatt ctatgcttaa aaaaatggat
    10261 agaaagacat tcactaaaaa tgacaaatcc atcattaatt ttaataagga acgttttgat
    10321 ttttatatag atgtaggact agattatccc atccctttct tgggggaagg tagagatgga
    10381 gaaaaagtag ttgtaaaatt tgttaattca tatccccatt taaaagtcat taaggcatct
    10441 ggaagagatt tagaaaagct ttattttgga tacagtatag taaaagtaaa ttcacttgaa
    10501 gattttttga aaaaacttga cgaaaagacg tttgttatat ttacatcaaa gaatggaaga
    10561 atattcaagt ctgttgaatt tcagtttaaa caaagtatta acaattttga aaatttgttg
    10621 attgttttcg gttcacctag caaaggtatt aaagaaatat atactaatta ccacactacg
    10681 aaaaattcaa tgtatctaaa tatgtttcca gaccagaaga ctcagactat aaggttagaa
    10741 gaagcaattc ttgggacttt aacattgttc aatcacttcc ttctttaaac caaagtgttt
    10801 gactttagtt atagtatcca tcgctatgaa aatataaatt tattaatggg attagaaggt
    10861 ttgttgctgt atccatgggt catagaaagt atagctcgcc aagacgagga agccttgcat
    10921 tttatcctag agtaagagcg aagagtgagg aggctagatt aagaaattgg cctgaacaca
    10981 gtgataatgc ttctctttta ggttttgctg gatttaaggc aggccaaata caagtaatga
    11041 caatagatga caaagaaaaa actcctaatt ttggtaaaca actcgacaat tcatcaacgg
    11101 taattgcaac tccaccaatt aatattattg gtataagatg ttataaaaaa gatctttatg
    11161 gagagaaagc gtttattgat gcttattcaa aggatttacc gaaagggtta gagagaaaat
    11221 ttaagactaa atatcatgaa aaatcttttg aaacacttga atcatcttta gattcgatct
    11281 tttctttttt tgcaattgca tcagtttttc ctaaaaaagc aaatctatct cagaaaaagc
    11341 catttgtttt tgagataggt gtctcaggaa aagataacaa aacaaaatat gaatatttaa
    11401 aaggtatttt aggaaaagaa attaaaatat ctgatgtctt tcaaaatggt caatatatag
    11461 acgtatcagg aattactagg ggtaaaggtg tagagggtcc catcacccgt tttggtgtaa
    11521 agcgaaaaca acacaaatct agaaaaagtg tcagagctct tggaacatta ggtcctatat
    11581 ctccagcagt ggtaatgtat tcagtaccaa gacaaggaca aagaggcttt caccaaagaa
    11641 cagaatataa taaaagaata ttattggtat catcaaatgg gtcttcccaa acaacattag
    11701 tagcttcgaa accaacaata aatcctccag gaggtttcaa acattttgga ttagttacta
    11761 ctgattatat tgtggttaga ggatcaatac ctggagttcc aaagagatta gttaaaatga
    11821 gacaacctat aaggaatact actaagaagg ttattgagcc taaagtcatt gaggtaattg
    11881 caaaatgagt ataataaata taatagaaat ggatggaagt aataaaagta ctgtaaccct
    11941 accgggggtt ttccaaactc catatcgccc cgaagtaatt caaaaagttt acaaccatct
    12001 taattcctat acctttcaaa gacagggtag atatcctgca gcaggacaga tggtaagtgc
    12061 agagtcccgt aatacgggat taggaattgc aagaattgca agagcacgag gagaaggttt
    12121 tcctagggct ggacaagctg ccggggtagc gagtgtaaga catggtagac tcgctcatcc
    12181 tcctgtttca tggaaaaata tctataaaaa agtaaataaa aaagagaaat tattagcttt
    12241 atgttctgct attgcagcaa caacaaacac cgaattgatt caaaggcgcg gccataagat
    12301 aaagaatgat ttacaattac caattgttgt ttcaaatgaa attgaatcta tagtgaaatc
    12361 aaaagattta gagaaaattt tgctaaaatt ggggttagaa gaggatttaa aacgtacatt
    12421 tattcgaaga aacaaatcgg tccacaaaaa tgggacaaat cgtagatctg cattaagtgt
    12481 cctgataatt gtaagtaatg ccgaaaagat aggaagattg aacaattctc ttcctgggat
    12541 tactgttaaa tctgttaaat caattagtgt attagattta gctccgggtt ctaaaccggc
    12601 gagattaact atttattctg aaaatgctat taaagaactt aacaatttaa aacccacatc
    12661 aaatataatt tcggagatga ttgctcaatg aagaaaaatt tgttcactct aaaatctact
    12721 agatcctcta aacaaaaaaa gaaaatttca caattacaaa aagatagacc attgattatt
    12781 gaaccatata ttaccgaaaa gtcattcaat ttgatagaaa aagaaaaaaa gttaaccttt
    12841 attgtggatg aacaagccga taaaaaatct ataaaacaag agttacatga tctttatgaa
    12901 gctgaaataa tcgaagttaa tacggcaaga acaatagatg gaaaaaaggc ctttgcaaag
    12961 tttaaggatt tagattctgc tcgcgacctt gctacaaaac ttggattggt ttaagaaaat
    13021 gaaaatgatt gttttttatt ctctcggtag atttaaaaat aagccattgg aggtataata
    13081 tcagattggt tagggagttt aagtttcgag gttataatca agatcaatta aaatcgatat
    13141 caatagagtc actcttacca ttgttaaatg caagacaacg aagatcattg aacaaaagag
    13201 tcggaaaata tatgaatgat cagaaacgaa cattgagaga agagatcaaa ctagcacgtg
    13261 atggaaagtt aaagaataag ctgaaaacac atgttagaga catgattata ctgccggata
    13321 tgataggtct ttcaattagt gtacacaatg gtaaagaatt ttttgatgtt ataattaaat
    13381 cagaaatggt aggacattat ttaggagaat atgctattac aaataagaga gttcaacatg
    13441 gctcacctgg agtcggttca tcacgatcta gtttgtatgt tcctttaaag tgattgattt
    13501 atgccaaact attcttatgc atatcaaaat tttgataaaa gtagacaagt acggtctgcc
    13561 cttcgtgaaa aggatatttc ccataaacat gcccgtgaga ttgcattagc aattaagaat
    13621 ctttctattg agaaagctag agattatctt gacgcagtat tacgaaaaga aatagctgta
    13681 ccgtttcgaa gatacaataa cgaagtagca cacaggtcaa atattcgaga tggattcagc
    13741 gctgggcgtt ttccatcaaa agcaactcat gaatttttga aacttttgga taatctagaa
    13801 agcaatgcag aatacaaagg catggacctt gataggttaa aaattatcaa tatagttgtt
    13861 cataaaggaa cacgactaaa gagattcacg cctcgtgcta tgggtagagc aagccccaaa
    13921 tttgatacac ttgtacatgt tgaaatggtc gcacaagagg gtgtaataac agaatgaatg
    13981 ctgcaaaaaa tgtaatgtct agtaattata aaaatatgga gctagacgag tatctaaaag
    14041 aaacacttag ggagacaggt tatggaggtg ttgatattca aaaaactcct ataggaacaa
    14101 gaataacatt atacgtaacc cgtcctggat tagtcatagg acgaaaaggc tcaggaatta
    14161 aagatcttac ctctaaatta gaacaaaggt tcggattgaa taatcctcaa atttcggtga
    14221 tggaaataga ggttcctgag ctcaaccccc aaattatggc caatagaata gctcagcttg
    14281 tggaaagagg aactgcattt aggagagctt ctatgtggac aatgaatact gtaatggggg
    14341 ctggagcatt gggtgtggaa attagtattg caggaaagtt aagaagtgaa agagcccatt
    14401 ttgaaaaaca ttcatcaggt gtggtaccaa agagtggcaa cgctgccgat aaaatagtca
    14461 aattgggcat aactgacgta ttaactaaaa tggggttaat gggaatccaa ctgcgaattg
    14521 cattaaaaaa tgaagtgccg ccagactttg aacttataga gtcaaaacaa gaaaatttac
    14581 agaatagtga tccacaatta gatcaaggca ccgataatga agccaataaa aatacaatac
    14641 aagaaaatac taccagaaaa aatagttcag aaagaaataa ccaaaatata aaaagacaac
    14701 aacaaaaaca aattcctaaa tccaaagata taaaagataa ctctatgaat cagaataaag
    14761 taaaagaaaa agggagtgtg ccaacgaata ctaaatgagt agaataaaac taacaactct
    14821 tcgagaaatg aatgatcagg atcttgaaga caaattatta gaattgaaag ctgatcttgc
    14881 taaattaagg tcagaaggag caaaaggaac attgaaaaaa gagagttcta gtattcgatg
    14941 ggctcgaaga gatatagcaa gaattcttac actcatcaat gaaagaaaaa ttaaaaaaag
    15001 agtcaagata tgagatcgat cgaaaatatc ctttatcatg aatggatcgg gttaaaaata
    15061 aaattattgg aatgttcaca atccagctta acaaatttat ctggtcaaat catttttgaa
    15121 actaaaaata tgctgattgt aagaaccttg aatggaatta agaaaattcc aaaaaaaatt
    15181 atactaaaat gtattttata tcttccttca accgcttgct ttataagagg taaccaattg
    15241 ataggaagac cagaagatag aattttaaag attaagagat gataatgaaa tatggttaga
    15301 aatataggta ttatggtttc acttcccaaa aaaacatgtg aagatcaaca ttgtcctttc
    15361 catggaagct tatcagtaag gggaagatta tacacaggta ttgtagagag taataaatcg
    15421 ccaaagatga tcgttgtctc cagacaatat aacaggttag ttaaaaaata caaaagatat
    15481 gaaagaagta aatctaaagt acatgctttt cttcctgatt gtattgatgt taatgaagga
    15541 caaaaagtca aaatagctga atgtcggcct ctttctaaaa cagtatcttt tgtagtcgtt
    15601 gagggagata ataaagatgg catctaaatc aagagcagtc tcagcgaaag gtgcatctga
    15661 atttcgtccc tatattacaa gggcactccc tgtcacagca gagcttgtat gtgctgataa
    15721 tactggtgcc aaagttcttc gaattgttca agtatcgaaa tatcatgggc gtcattcaag
    15781 aatgccctca ggggccgtag gcgaccttgt aactgttact gttaagaaag gtccagccca
    15841 attaagaaaa cagatttttg gggcagtcat aattagacaa aaatatccta taaggaggct
    15901 aagtggctta agacttacat ttgaagacaa tgctgctgtt ttagtgacac cggagggtga
    15961 aattaaagga actgatataa aaggaccagt tgctgctgaa gcagcagaaa aatggccaag
    16021 aatagcaaat ctggcttcat caattgttta agattatagt gattgtttat gacaaataaa
    16081 aatactaccc tcaatataaa aaaacatata cttgataaac atattcgttc ccccctttca
    16141 gaaaatttac ggaaggaata caataaaagg aatgcgcggg tgatcacagg agatactgta
    16201 aaggtattaa gaggagaata caaaaatgtt gaaggtaaag tagaaaaagt aaaaactaga
    16261 agatcaactc tttttattga agggatccaa agagaagcct caaaaggagg taaagttaag
    16321 gttcaaatac attcttctaa cgttattatt acatctttta acttgcatga caagaatcga
    16381 tctaatgtaa ttagaaaagt taaaacagct aacctggaaa aaaaagggat aaatattaaa
    16441 cctaatataa ctaaaactcg aataaaaaga aacttaaatg ttaaaaaggt tcttaaacca
    16501 caacaaaaaa gaataacaga ttcaaaccag agtcaagata ataaggggga aaaataatga
    16561 tgataatgtt agaaaataaa atgtttttag tggaacaaat ggagctagta taaataatgg
    16621 ctaataaagg aggaagaact actgtaaaac gacaattagc tccagtattt tgggatataa
    16681 atagaaaaca agggcgtttt attgttagga catatcccgg aacacatgca aaaaaatatg
    16741 catattctat aggtgtaatt ttacgagatg tgttaaaggt cacaaaaaac atgcatgaag
    16801 ctaaaaaaat tgtcaatgca ggtaaagtca aagttgataa tgtaatcaga tacgatgtta
    16861 attttgccgt aggattaatg gatacaattg agttagtttc atctcaacag aaggtttaca
    16921 gattagttcc cagcaattca ctgttattat ttccaataga aataagtgat aatgaaaaga
    16981 cactaaaact tgtaaagata aaaactaaaa atattattaa gggagggaaa attcaatatg
    17041 gtttccatga cggtaaaagt gtcatttctg agaatgttta ttcagtcggt gatacatgtc
    17101 ttataaaagt accagaatta gaaattattg aaactctaaa attacaatct gattgtcttg
    17161 ctatattaac acgtggtgat aatgctggta gtataggaaa aatacaagaa ataaaagaag
    17221 gaacattttc tcttccgaaa cgtgtaataa ttagtgtgga ttccagattg ctagaaattc
    17281 cgattgatat gattatggtt attagtgaac gcgataataa taatccaaag ataaaagtga
    17341 attaaagatg tctgaaaata ataatgattc aaatgaaaaa atgaaaagga tagatatcga
    17401 aaaaattgtg ataaatatag gcgtagggaa gtctggcgat cctttcgaaa gagctaagat
    17461 ggcgttggaa gaattgacag gtcaaaaacc tagtccgagg ggagccaaaa agtctgttag
    17521 agattttggt attcacaaag gtgaaccaat tggagcgatg gtgactttaa gaagacaaat
    17581 cgcattggac atcttaaaaa gaatttttct cgctaaatca aattctttta aaagaacttc
    17641 catagataat aatgggaatc tttcgattgg aattaaagaa catatagata tacccggaat
    17701 aaaatacaat cctgatatcg gaatttttgg aatggatgta tgtatctctt tgaaaagacc
    17761 tggttataga atttccaaga aaaaacatcc tgacaagatc ggtaagaacc atagaataac
    17821 aaaagaagat tcaataaatt ttttccaaaa aaattttgga gttgaagcga tataatgcct
    17881 aagccacgtg aatattctga aacaggtaga aaaaaagtac cacatggtaa aggaacacga
    17941 tggtgcaaaa gatgtggcgc ctttaatgga ttaattcaaa aatatgatat aatgctttgt
    18001 agacaatgtt ttagagatgt agcaattaat ctaggattca agaaatatga ataaaatata
    18061 tattgatatc gaaaaaagaa agaaaataga gagagaagca aagtatgata ataataaata
    18121 ggattaatat tgtgtgggag aacgaattct aaatgccgtc attaaatatt gtcgcaaatt
    18181 tgtttaatac tttatacaac aacgaaatta gaagaaaaaa agaatgttta gttattccta
    18241 catctaaatt tgccagtgaa attctcaggg ttatgcaaaa gtatcgatat ataggagaat
    18301 ttgaactgat cgatgacaat agaatgggga aatttagaat tcaattatta gctaaaataa
    18361 acaagtgtgg aattataact cctaggtacg gtgtaaaaaa gaatgaattt ctcaattggg
    18421 aaagacagtt tttacctgca tataaccttg gaatattatt ggtatcaaca agtaagggca
    18481 taatgtctca tactgatgct caaagtcaag gattaggagg tgttcttata gggtatgtct
    18541 actagcaaag aagttaatca atctggagaa gtcataatcc cagaaagtgt caaggttacc
    18601 aaggaaaaaa atacaataat tgttgaaggt aaaaatggca aaatatctaa agattttact
    18661 aaagtcccta taacactagt ttttgaaaat aacaagatca tcattcaccc tcctggtcaa
    18721 cgaagaaaag atttggcgtt aataaacact gtaaaaagta ttatttctag tatgattaaa
    18781 ggagcagaaa ctggatttac ttataaactc aaaataattt tttctcattt tccaatatca
    18841 gtcaaagtaa aagataaaaa agtaattgtt gaaaattatt ttggagaaag atcttctcgt
    18901 atatctaaaa ttgttggttt aactcaagtc gtagtaacgg gggaggatgt tatagttaaa
    18961 ggacccagtt tagaagatgt ttcacaaact gctactaata tagaatcttc taccaagata
    19021 aaaaataaag atcagagagt atttttagat ggcgtttatc tatatgccaa agaaaatgga
    19081 atgaattgat acattttaac tcttcccaac gttaatctat aatttttaaa cttagataga
    19141 atgcaaaaca ttattgggtg tataatttta atagtgtaaa ttaaatctga ttcgaagatt
    19201 tgtttattgc aataagaaaa catttttatt ttgataaaaa aaatgatagt tgaatgccgc
    19261 tgtagctcag catggtagag caaatttaaa gcgaaatacg ctttagagct tagctggctg
    19321 ttaaccagtg ggtcatcagt tcgagtctga tcagcggcgt ttattttttt taataacaac
    19381 aagatcactt ttacactatt cttcattgca taaaagattg tattgatgtt atgttacctc
    19441 atttatatat ttattatcgt aagattaaat tacaataaga tcagggaaag aaacatttta
    19501 tcctttttaa tatcatctca ttgttatata aaaaaatagt atatatttca ttcattaatc
    19561 tttaataatc tgattcttga ttctattttg atgaaagctg ttatacttgc cggtgggtta
    19621 ggtaaaagat taaaaccttt aacaaatgat cgacccaaac ccatgataga ggtactaaat
    19681 gt
//
LOCUS       DASVEV010000134        28623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1019317, whole genome shotgun sequence.
ACCESSION   DASVEV010000134 DASVEV010000000
VERSION     DASVEV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 28623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28623
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019317"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..99
                     /locus_tag="VF222_05955"
     tRNA            26..99
                     /locus_tag="VF222_05955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60..62,aa:Ile,seq:gat)
     gene            complement(456..1853)
                     /gene="gvpD"
                     /locus_tag="VF222_05960"
     CDS             complement(456..1853)
                     /gene="gvpD"
                     /locus_tag="VF222_05960"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD P-loop
                     domain-containing protein"
                     /protein_id="HEX7142003.1"
                     /translation="MNKNYDTSFKIPDDLLQFLNNETYSLLIKGEPGAGKTALCLTIL
                     KALNIKNNFFYISTRVSPNKLFYYFNWINKYLKIKSSNSHNKSSKNINSLEPFFEDAR
                     LDEPESLFERITNQLMDVRSPLIIVDSWDGIASFMDRESRLNNERVLQTWLERAGGRL
                     ILVNEGNELTTLDYLVDGIITLKFNQFSHIILREMNLTKLGGIDIKKNSFLYTLKNGI
                     FKTYESSNHYDHILDHILSSPKNKSITHKKIVKIDSGHFSLNKDLNGGFTKKSIVLLQ
                     HDPNIEIRYTILFLFNFITNCISNSHMLLLDNKIARDTTSILNFIRSNIVDANDYDKF
                     VKLIDFNLDTNSDNLDNTLNTDLLINPISEETKNIKSNSMIFGILNFDRYKNIDEYKL
                     INTLKDKLDLSFLLFQTGIKSELTPRLFDYKITIKNFNDIVCIHLTHPYVRVYGLELV
                     NIENSNFSIRIDPLI"
     gene            complement(1950..3158)
                     /gene="dph5"
                     /locus_tag="VF222_05965"
     CDS             complement(1950..3158)
                     /gene="dph5"
                     /locus_tag="VF222_05965"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HEX7142004.1"
                     /translation="MKSTKELYFICPECITFRINKIRGNNSELNTGMLCFVGLGINGY
                     SGLTLLAKETLQKCKFIYIERFTSYIDDDDIRQLELLLNTDNKRTQIIPRWFIEDARA
                     IISQSKDDSVAILTYGDPFMATTFTELYVRAKKNLIEIKIIHGASGISSLIGEIGLHN
                     YKFGRTTTIMSDPMSAISVYNIIYENLRVGNHTLILTEYNNNNNNNNNNNENDFFFLD
                     PSRAIDMLLEVEENINYKIISDELFIIVASRIGNKDQNIIGGKIKSIKKIDFGKGPHS
                     LIVTGHLHFTEIDSITSLYPNKDPPLDNTQFFRHIAITMLEKYLPKAKNALKNLKSYI
                     ESERNSSAILDEQHKGMYHVLENAEYYLLDAERFFNQHKFELGILSIGYAEGLIDALR
                     FQKNINPWLS"
     gene            complement(3097..4212)
                     /locus_tag="VF222_05970"
     CDS             complement(3097..4212)
                     /locus_tag="VF222_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7142005.1"
                     /translation="MVSSNYAIELLKTSINLYTPSRSEGPLANLLKDKCVNELGFEQA
                     HIDSVGNIIATKGSGYPRILLCGHMDTVPNKVPVRIENGFLYGRGASDAKAPLISMLL
                     AASEFPKQNGTIIFAGVVDEEGNATGSKQLVKNKLDIDYAIFGEPSGIENITISYKGR
                     LALKLICDVLDSAHASAPWLAKNSIEEVYNFWNAIKSTLHTNYEKEKRSNSVSCCLTE
                     ILGGSSHNVTPQKCKITMDIRIPNGTTCKQVFYSIKTLIAKISKENGIKTNFIIEDFT
                     EPFEADFSSPLVRSLVLSILDICKKRPALLRKTGTGDMNIIGNAFKIPVVTYGPGDPH
                     SSHSINEHVSIEEYLLGIEVYKRALFHMSRMHNIQNK"
     gene            complement(4220..5095)
                     /gene="lysX"
                     /locus_tag="VF222_05975"
     CDS             complement(4220..5095)
                     /gene="lysX"
                     /locus_tag="VF222_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX7142006.1"
                     /translation="MTEGRNTSSSLTLLYDTIRWEEKAIYEAARKKGIEIKKIDCKDI
                     VLDLNTLSCEYTNQTIIQRCVSYFKNLHSTAALEGLGANVINPLNTAVTCGNKLFTHM
                     LLQKKGIRTPKVITAFSPNSALSALENFGYPVIIKPTIGSWGRLIALLRDKDAAKAVF
                     EDREHMFPLYHVYYLEEFVSRPPRDIRAIVVGDVVVAAIYRYSESDEWKTNMALGGRA
                     ENCPVTDELEEICIKSSQAVGGKIVGVDLMESSKEGLLVHEVNNTTEFKNTVKITEIN
                     IQDMIVDYSLKYQIN"
     gene            complement(5092..5259)
                     /gene="lysW/argW"
                     /locus_tag="VF222_05980"
     CDS             complement(5092..5259)
                     /gene="lysW/argW"
                     /locus_tag="VF222_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HEX7142007.1"
                     /translation="MVKCEECDAEIKIPEDSIEGEIVSCPDCGSSYELVKIEGGFTTK
                     PAQVVGEDWGQ"
     gene            complement(5267..6475)
                     /locus_tag="VF222_05985"
     CDS             complement(5267..6475)
                     /locus_tag="VF222_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7142008.1"
                     /translation="MILKSDDPNYYAHQYNNVGHEKKKIKILDSTLREGEQHPGVSFT
                     IKQRIQIAWMLDSFGVDQIEISPVVSSDHLEATKTIIKQGLKADIVAHVRAIKSDVDI
                     ALKTNSTWIATYLGISDIHLSAKLKISRDEAKRRAIEVVEYIKSHGLKCRFTMEDASR
                     TEPSFLLEMCKEMNRLGVERISIPDTVGIMRPHGMYNLVKMVYDKIDTSKSSLDVHCH
                     NDVGLALANALSGCEAGADQIHTTIDGFGERTGIPSLAEASIALTLIYGSENNLRLHM
                     LKDLSRTISQYTSLEIPESKPIVGDSAYKHKAGTHLAAILRNPAAYEIIEPRLVGNRR
                     KIVFGELAGKNGAAYLLSLLGLDTSIREAENLAKGLKELRMGDVLELYLDEKLERKIL
                     NEETFNVKKE"
     gene            complement(6577..7008)
                     /gene="lysM"
                     /locus_tag="VF222_05990"
     CDS             complement(6577..7008)
                     /gene="lysM"
                     /locus_tag="VF222_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator LysM"
                     /protein_id="HEX7142009.1"
                     /translation="MLNNIDSFDEKIIKILQKDARKPFVEIANTIGLSESAVRRRIKN
                     LTDNNVIKKFTIEINNKGKTSAITLISVASSSDTSTVTSKLLNLEGVDVVYEITGQYD
                     IAAIISAPEISEINSYIDEVRKIEGVSDTNTVIILKTLKNT"
     gene            complement(6998..8176)
                     /locus_tag="VF222_05995"
     CDS             complement(6998..8176)
                     /locus_tag="VF222_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7142010.1"
                     /translation="MSEDNYLGNLYQRFPINISKGKGATVWDVSGKEYIDCMGGYGVA
                     LIGHCNDRVVNAIKKQSEKLITCHMSLYNNVRLEFLEKISKISPKKLSKVFFSNSGAE
                     STESALKFSRKFTGKSGIIAMTGGYHGKTFGALSVTHNEKYRKSFQPLLEGVKFVPYS
                     NPSKIEESLDTSIGTVIIEPIQGETGIIVPSDGVLQQIRKICDQNDLVLIFDEIQTGL
                     GRTGRMWAGEHWSTTPDIMCLAKGIAGGLPMGLTLCKPEILDAMKVGEHSSTFAGNPL
                     SCSAGIATIESIIEESLVENAAKIGNIFKNGLFQLKENHRIVRDVRGLGLMLALELRF
                     DIKDMLFDGIKEGLLMLYSGRNIIRLLPPLVLDDVKVAKTLSIMDKLLTKEEERKDVK
                     "
     gene            complement(8173..8943)
                     /locus_tag="VF222_06000"
     CDS             complement(8173..8943)
                     /locus_tag="VF222_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate/[LysW]-glutamate kinase"
                     /protein_id="HEX7142011.1"
                     /translation="MDGLHSSTISDLKNILKQDKVVLVHGGGKDVTIIGNKLGKEQKF
                     IVSPGGVKSRYTDKETAEIYTMVMSGKINKTIVRLLLNNGISAAGISGIDGSTIEALR
                     KKKLVVVNDKGRKMMIDGGYTGKINKINTLLIEKLLEGNFVPVVSPIALSEETEFLNV
                     DGDRAAAYIAGGISADKVIFITNVNGLILNDELIKEMTYDKAKESLPKIGHGMEKKVM
                     ACIESLDMGVKEAIIASGDVENPISSALFHTNCTVITK"
     gene            complement(8970..10022)
                     /gene="argC"
                     /locus_tag="VF222_06005"
     CDS             complement(8970..10022)
                     /gene="argC"
                     /locus_tag="VF222_06005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7142012.1"
                     /translation="MKVGVIGASGYVGGELLRLLVTHPKVTVDVVTSRQHVGEYVHRA
                     HPSLKGFIDLTFSSMDINKIADSCDLVFVSVPHGNANKIVKNLYERDIKIIDLSADFR
                     LKNPNDYIKWYGWEHPFPDLLSKSVYGVPELHREEIKKAKLVSCPGCMAVTSLLALKP
                     LIENDLIDTDHIVVDSKIGSSGAGAQANAGTHHAMRYGVIRPYKPAKHRHTGEIIQEL
                     CRLSKKVIKVSMSPHAVNLVRGILTTNHTFLTKTIGELDLWKIYRQSYKNEPFIRFIK
                     DKKGLYKFPDPKFLIGSNFCDIGFDIDEDNNRVVALSASDNLMKGAAGSAIQNMNVMF
                     GFDEMDGLKLTPLTPV"
     gene            complement(10106..10963)
                     /gene="lysX"
                     /locus_tag="VF222_06010"
     CDS             complement(10106..10963)
                     /gene="lysX"
                     /locus_tag="VF222_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX7142013.1"
                     /translation="MELSIIYDKLRFEEKELYDKATNRGITTHLIDAKPISFSTDRNQ
                     NNTINGDIFLQRCLSHYRGLYITYCLEFLRHKVINKYDVNEICGNKLRTSLILAKSEI
                     PSPKTYFTFSAESFKELTNKLGFPLVLKPLVGSWGRGVFPIRNEETVNIIMEMREEND
                     SAFSRIYYVQEMINRPPRDIRCIVAGDQIITAVYRYSAINEWRTNVARGGHTGLAPVT
                     KELEDIVIKASNAVGGGILGVDVMEDENKGFVIHEINSNVEFRGASQVSHVDIANSMI
                     EYVLTVYKK"
     gene            complement(10974..11159)
                     /gene="lysW/argW"
                     /locus_tag="VF222_06015"
     CDS             complement(10974..11159)
                     /gene="lysW/argW"
                     /locus_tag="VF222_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HEX7142014.1"
                     /translation="MSNRMKIECKDCGGELNILEDVVVGEIVSCPDCGAEFEVTSNSA
                     GKVDFKPAESVGEDWGE"
     gene            complement(11149..12366)
                     /locus_tag="VF222_06020"
     CDS             complement(11149..12366)
                     /locus_tag="VF222_06020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7142015.1"
                     /translation="MVKDKCILAYSGGLDTSVCIKYLQLLHNLDVITVTVDCGQNDDF
                     KEIENRSKEIGAIQHYYVDAKEEFVNEYITHCIKANGLYENQYPLGTALARPLIAKKV
                     VEISNKENAKTIAHGSTGKGNDQVRFDITIKSFNPNLRIIAPIRDMNLTRDIEIQFAK
                     EHNIPISTEVKKYSIDMNIWGRSIEGGYLENPFVEPPEDCFKFIKFNNSEVCYLEIEY
                     EKGLPITADGKRMNLIDLINYINFKAGSCGVGIVDHIEDRIIGIKSREVYECPAAIVL
                     IQAHQDLEKMVLTSHELRFKHIVDQQWSWMTYSGLWLDPLLNDLDKFIDATQNRVTGK
                     VKMRMQNGSTRIVGRESKFSLYKNDLATYSSDSIFDQQLAKGFVELWGLQSITANSIA
                     INLNKKENKRNEQ"
     gene            12514..13431
                     /locus_tag="VF222_06025"
     CDS             12514..13431
                     /locus_tag="VF222_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX7142016.1"
                     /translation="MYRRYTIDEIKNKVIETLANHEEGMSSMELADAIHVNRMTITKY
                     LNVLSAIGLIKMKKIGSINIWTRESGVDDIEFPLNYIHIQQKFLTALLGYNKKESSKI
                     LLNIIYSNTEVLKVLKEIIIPSLNTINGLYNRGRLGKTELIYLNNLLFDLLIVIEIHY
                     KNIDHNKNIHNIFIAGNEDQILYSKILSLVSEVSNCDTIYLGNIGQHIDPFFDIDLQS
                     HITRFWSHKKGTKIIFLCCSDESSLKFLFSTSDYIKRKMTEIIKIVLFVNPEILNLTK
                     DLKFDFITSDLNLLIIWLEELIKQIRTKI"
     gene            complement(13467..14303)
                     /locus_tag="VF222_06030"
     CDS             complement(13467..14303)
                     /locus_tag="VF222_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7142017.1"
                     /translation="MVNFSITIDSFTKIESLENTIKSLVELGIRNIEVSGDPSNSYSR
                     KNSELFNTFSVSVLGVTGKWTGSKGMIPPILLTSDPQILSYSKNYIKECIKICNFFGG
                     SIFNVCLLSDYDLKIDQNHEMIPNKEKKKLLKRSVTVLSELTNFSKDYGVHLLLEPLN
                     RYSTPFCCNTADAIFVTSNLNNDYFSILLDTYHMNIEEKNFSDSIKKSKSFLKHVHFA
                     DNNRKMPGLGHINFNPILKTLKKIQYNNYIGLEPMVDRNYKVEIIQGLKFLNKLCNKY
                     HI"
     gene            complement(14326..15657)
                     /locus_tag="VF222_06035"
     CDS             complement(14326..15657)
                     /locus_tag="VF222_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX7142018.1"
                     /translation="MIEHVIVIDIVGLEEKHLNSKLLPTISALAEKGESSKMKPVFPA
                     VTSTVQTSFLSGEYPNRHGIISNGFMDRNTYNVLFWEQYNSLVQVPRIWDFIKSKNIN
                     FKTAVLFWQNTLYANSDIIITPKPIHLENEMKMWCYSKPVGYYEEIMKQIGEFDLSSY
                     WGPLASSLSSEWITKAAQYTIEKSRPNFMFTYIPHVDYSAQRFGKESNQVSDDLVLAD
                     SIVEKIIDSTKKSKTYENTQFIIFSEYSFNNVNCAIPINIILRNNGLLNVRKIGDKEY
                     VDFEFSQAFAVVDHQIANIYLKSPQDKERIVNILKKISDIDIIITGVEKKFFNIDHER
                     AGDIIVVANRDKWFSYYWWYDENMAPSFTRMVDIHRKPGYDPLELFFDSKKRSIPFDT
                     GLLKASHGRPVNMTTQEGYSLYASNIRSNLKKDEMNSLDVVQIGKYLMNLL"
     gene            complement(15752..16645)
                     /locus_tag="VF222_06040"
     CDS             complement(15752..16645)
                     /locus_tag="VF222_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX7142019.1"
                     /translation="MSSTIINYFRLVRIPNVFTTFSNILLGYIFFTSIDYFDYFVILK
                     LISISAFLYIGGMILNDYFDIKIDKKERPGRPLPSNKISKKNALILAFIAFSYSLIIS
                     FIMGWYTFIITVIMVTLIFSYNRFSKKTIWGSINMGIIRSLNVLLGASQSLFLINGGM
                     FETGFIIPLLSEFLYVCAITILSRNETKDFFFTLWNNIPFIIIYVLIFSLCIFIILGA
                     FNYQSLIPLIIFSCFIIYSHIGLFKKIATTPKSVSQLITMIVFLDIIFITDTLGVYYS
                     LILALVLIIPVIFLSRRLYMT"
     gene            17436..18446
                     /locus_tag="VF222_06045"
     CDS             17436..18446
                     /locus_tag="VF222_06045"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7142020.1"
                     /translation="MKIQAYAAHQIAGQLEPFEYELGPLKSDEVEVDVEYCSICHSDL
                     HMLDNEWGITQYPFVPGHEIIGRVSAIGNMVEHLQIGQYVGVGWRSRSCLICDQCTSG
                     HHNRCLKAEDVIVGRYGGFANKVRCQAVWAFPLSANIDIKTAGPLFCGGITVFNPIVQ
                     NNIKPPNHVAVVGIGGLGHLAIKFLKAWGCEVTAFSSNPEKENEVRQFGAHYFLNSKD
                     PESLKLSVNRFDMVLVTTNVELDWNSYLNTLRPGGKLHVVGAVPSLKVDVFPLISGEK
                     SIGGSPIGSPATINAMLDFCSRHDITPVTEEFPLSQVNEALAHLKAGKARYRIVLRND
                     IK"
     gene            complement(19460..19858)
                     /locus_tag="VF222_06050"
     CDS             complement(19460..19858)
                     /locus_tag="VF222_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7142021.1"
                     /translation="MKGYKTNIEKDALDNNNFRTVLYTSKHVQLVLMSLRPQEEIGEE
                     IHYENDQFFRIEGGHGKCIIDDNEYEIKDGDAIVIPTGSKHNIINIDTQTDLKMYTLY
                     APPHHKDGILRYTKKEAEENAPEFDGKTTE"
     gene            20301..20477
                     /locus_tag="VF222_06055"
     CDS             20301..20477
                     /locus_tag="VF222_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142022.1"
                     /translation="MPDCEMCGTFTAKSYNVDLRITSNFDREEEPFERYSIVCSDCRN
                     KILNVFPKVEDEFF"
     gene            complement(20645..21367)
                     /locus_tag="VF222_06060"
     CDS             complement(20645..21367)
                     /locus_tag="VF222_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7142023.1"
                     /translation="MKNNDQPKNISNKSDKTKSDVKHKYLVEIVGTFILVYAIASAAT
                     VYSDSGQLGVIGIGLVHALVLTAIIYAIGYRSGAQVNPAVTIGLLVARKIGDKEAALY
                     IICQIIGGVIGAAVVYLMFGSAMSASVTLPSENNVMRAFVLETVMTFTLVYVVLATTT
                     AKNFRIAPMAGVAIGFTLGFNVIFGGSITGGSLNPARSFGPALMTGNFDYNWLYWIAP
                     ILGGIIAAGVYKALHKDTDLPE"
     gene            complement(21640..21888)
                     /locus_tag="VF222_06065"
     CDS             complement(21640..21888)
                     /locus_tag="VF222_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142024.1"
                     /translation="MRDLYNRKSQELQNIDPTLLYRFITAANKEYIENLKTNNPNIEK
                     NIIHKPFWLNELRTTIHSFLSNKEKQQKEDKDKLLMLI"
     gene            complement(21975..22076)
                     /locus_tag="VF222_06070"
     tRNA            complement(join(21975..22019,22037..22076))
                     /locus_tag="VF222_06070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22038..22040),aa:Leu,seq:caa)
     gene            22673..23674
                     /gene="egtD"
                     /locus_tag="VF222_06075"
     CDS             22673..23674
                     /gene="egtD"
                     /locus_tag="VF222_06075"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX7142025.1"
                     /translation="MSRSYNKNNKKTILPEDFAKEVKNGLERKKKKLNPKFFYDKRGS
                     ELFEQICVQPEYYLTLTEYKIIIENINSLLKYYDNKDICIVELGSGSSKKTKILLNYF
                     LKKQDGNLHYFPIDISQEMLYKSSLKLQSDLPKIINHPIASDYFEGIRTVTKFIGSQK
                     NIPNKKLILFLGSSLGNFEPKDAIIFLRNIREMIDKEDTLLIGFDLQKKKNILEAAYN
                     DGEGITAKFNLNILERINKELDGEFDLENFGHLAFYNQVKNRIEMHLISKVKQKVKVN
                     KLNRVFDFEKGETILTENSYKYSLKSIEQLASKSHLRVERNFLDKNEWFNLALLTPF"
     gene            complement(23682..25025)
                     /gene="egtB"
                     /locus_tag="VF222_06080"
     CDS             complement(23682..25025)
                     /gene="egtB"
                     /locus_tag="VF222_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX7142026.1"
                     /translation="MTKVINKVVEDLISEFTEVRKLTNDLFKTLSIEDAVIQSDPFGS
                     PPNWHIGHVTWFFHKILLKYGEKLNLENINLDYLNSYYQKFEKILPKYDRGRFPRPTV
                     SESLHYRKYLDKKVLLFLEKIEAFNSVTKEIHYDLQLANQHEMQHQELMVYDFQNYFE
                     RFPDSNDKYCPFKINDHFDKKAQSKRHNKEMVKIPGGIFDMGFYGDGFSYDNELPEHK
                     VYIYPYEIDLTPVSNSDFINFIEEGGYERYEYWLSDGWDVVQEFKWNSPLYWFKKDDV
                     WMKKDFFGTSTINPDEPVINVSYYEADAYARWAKKRLPTEAEWEKAASWNEDLKRKTR
                     YPWGDEILPLPVHANLLESFLWRPSLCGSYEKGKSFFGCHQMIGDVWEWTSSEYSLYP
                     GFKSRFSEYTDKWAINQKVLRGGSFATPRRQIRNSYRNYFKPHERIQFSGFRCAK"
     gene            25173..25973
                     /locus_tag="VF222_06085"
     CDS             25173..25973
                     /locus_tag="VF222_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEX7142027.1"
                     /translation="MVKSLVENSFKSIDEGLYNITNIDYSHEPLNLKKNILDLKAKNK
                     IPIITEIKFSSPSKGVIMNREGTDLLSISREMIKAGAAGLSVLTQKYLFNGSLNNFVM
                     IRQDTSIPMLMKDIIVSEIQLDCAKKIGADYILLIMNIFDKNLAEGDLDKFVRYAHKK
                     GLNILFEVHHEDEFNEILKFNLEKNDLIGINNRNLDNLKIDLEITEKLLKKFDKGKNV
                     IVSESGINNTNQIKQLKNAGADAFLIGTSIMEKNKQIAIKIKELVNVI"
     gene            26041..27240
                     /gene="trpB"
                     /locus_tag="VF222_06090"
     CDS             26041..27240
                     /gene="trpB"
                     /locus_tag="VF222_06090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7142028.1"
                     /translation="MKRYNFPFNGKFGNFGGKYIPETLVPAIEELEESYKKFTNDPEF
                     KKELKDYLENYAGRPTPIYLAKNLTEYAGGAKIYLKREDLLHGGAHKTNNTLGQCLLA
                     KKMGKKRIIAETGAGQHGVGTALASAVLGLKCEIYMGLKDTERQKLNVFRMKLLGSQV
                     HPVKSGTQTLKDAINEALRDWITNVENTYYVIGSVVGPHPYPMIVRDFQSIIGKEIKK
                     QIRKITGTLPNAIVACVGGGSNAIGSFYPFIGDQEVSLYGIEAEGSGINTGKHSATLS
                     AGSTGILHGMQTYLLQDKMGQISPTHSISAGLDYPGVGPEHSFLKEIKRVHYKSCTDE
                     DAIKAFMILSEKEGIIPALESAHAISYGMKLAKKMKKSETIVITLSGRGDKDVEVVQN
                     YLNGIRD"
     gene            27244..28083
                     /gene="trpA"
                     /locus_tag="VF222_06095"
     CDS             27244..28083
                     /gene="trpA"
                     /locus_tag="VF222_06095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7142029.1"
                     /translation="MHKSKSRPSKENRIDSKFNELKSKNQKGLICYVVAGYPDIKTSE
                     YIITSLINGGADIIEIGIPFSDPIADGSTIQDAIQSSLISGTTPDMCLELASRIRKTF
                     PNIPLIIMTYSNILYKKGYIQFAEKAKENGIDGLIIPDIPIEESKEYLHTAQNTGLST
                     IFLVSPNTSEKRLKMISRICTGFLYAISIYGTTGERKGFDEYTIESIKRVKKVTAADE
                     LPLAVGFGISNPQHVKYMIDAGADAVIIGSAIIKKIKEIEDKKLLFNTLNKFIYELKN
                     SCR"
     gene            complement(28121..28447)
                     /locus_tag="VF222_06100"
     CDS             complement(28121..28447)
                     /locus_tag="VF222_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142030.1"
                     /translation="MSVGDFVLACSIDDSPSYFTYDNDDTMIIIQSKSSALASHSDFY
                     YIEPYIELLISHESLHFVIKRIEGTRESEMLDDLEVIVYRNTKNYQVTINNMLFASDN
                     SGLVLE"
BASE COUNT         9866 a         4528 c         4094 g        10135 t
ORIGIN      
        1 caccaacact agtgtgtgct aaattgggcc gatagctcag cttggtggag cgttcgactg
       61 ataatcgaaa ggtcgagggt tcaactccct ttcggcccat gattttaata atacaaacac
      121 aaagccttac cgcaaagata tggtaaagta atacaataat aaaactttga tattcaaaat
      181 tttttaaaca aattccattt ccatctattt tttttaaaag ataaatttat cctatatgtg
      241 tagacgtatt ataatgcagt gcgacttgac tgcatatcaa acttctcata gttaatagaa
      301 tctgcctcat tttttttcag gacgaagggt tattttctca tatcatttat cgcaattttt
      361 ttcttcagaa taatgagtaa ataatttgag aaagatgaat ataacgctcc tttctattat
      421 aatattatat taattgtaag aaatgtttaa atgatttata ttaatggatc tattctgatc
      481 gagaaatttg aattttctat attcactagt tcaaggccat acactcgtac atatggatgt
      541 gttaaatgga tacatactat atcattaaaa tttttaattg taattttata atcaaataac
      601 cttggagtta attcagactt aattcctgtc tgaaatagaa gaaaagaaag atctaactta
      661 tctttcaagg tattaatcaa tttatactca tctatatttt tatatctatc aaaatttaaa
      721 atgccaaaga tcattgaatt tgatttaata ttcttcgttt cttcagaaat aggattaatt
      781 aataagtcag tgtttagagt gttatctaaa ttatcagaat ttgtatccaa attaaaatca
      841 attaacttaa cgaatttgtc gtaatcatta gcgtctacta tgttcgatcg gataaaattt
      901 aaaatagaag tagtatctct ggcaatttta ttgtctaata gtagcatatg ggaattacta
      961 atacaattag tgataaaatt aaacaaaaac aaaatagtgt atctaatttc tatgtttgga
     1021 tcatgttgta ataatactat agatttcttg gtgaatccgc catttaggtc tttgttaagt
     1081 gaaaaatgac cagaatcgat tttaacaatt tttttatggg taatagattt atttttaggt
     1141 gaactaagaa tatgatccaa aatatgatca taatgattac ttgattcata tgttttaaaa
     1201 attccattct ttaaggtgta taaaaatgaa tttttcttta tatcaatccc tcctaacttt
     1261 gttaaattca tttctcttaa aatgatgtga ctaaattgat taaatttgag ggttataatg
     1321 ccatcaacaa gataatccaa agttgttaat tcgtttcctt catttactag aattaatctt
     1381 ccccctgctc gttctaacca tgtttgtaaa acacgttcat tattcagtct tgattcacga
     1441 tccatgaatg aagctatccc atcccagcta tctactatta tcaatggaga ccttacgtcc
     1501 atcaattgat ttgttatacg ttcaaaaagg gattctggtt catctaaccg tgcatcttca
     1561 aaaaaaggtt ctaaactatt tatgttttta gaagatttgt tatgactatt cgaagattta
     1621 attttaagat atttatttat ccaattaaag taataaaata atttattagg tgatactctt
     1681 gtagaaatat aaaagaagtt attcttgata tttaatgctt taagaattgt caagcatagt
     1741 gctgtttttc ctgctcctgg ttctcctttt atcaataatg aataagtttc attatttaaa
     1801 aattgtaata gatcatctgg aattttaaaa gacgtatcat aatttttatt cattgcccca
     1861 accccacttt agttaataat tattctgtta taataatagt tctccaaatc aaaatataaa
     1921 aaatatcctt aaaataatga ggtattaaat taagataacc acggattgat atttttttgg
     1981 aaacgtaatg catctattaa tccttctgca taccctatac taagtattcc taattcaaat
     2041 ttatgttgat tgaaaaatct ttcagcatcc aataaataat attcggcatt ctctaataca
     2101 tgatacattc ctttatgctg ttcatcgagt atagcagaag aatttctttc actttcaata
     2161 tacgatttca aatttttcaa agcattttta gcttttggta aatatttttc taacattgtt
     2221 attgcaatat gtcgaaagaa ttgagtatta tccaagggag gatctttatt gggataaaga
     2281 gaggttattg aatcaatttc tgtaaaatgg agatgacctg ttacaatgag agaatgaggt
     2341 cctttgccaa aatctatttt tttaatagat ttaatttttc ctcctatgat attttgatct
     2401 ttatttccta ttctagatgc cactataata aataactcgt cagatataat tttataattg
     2461 atattctctt cgacttcaag aagcatatct atagcccttg atgggtcaag aaagaaaaag
     2521 tcattttcat tgttgttgtt attattatta ttattattat tatattcagt taggattaaa
     2581 gtatgatttc ctactctcag attttcataa ataatattat agacacttat tgcagacata
     2641 ggatctgaca taattgttgt agtccttcca aatttgtagt tatgtaatcc aatttcaccg
     2701 atcaaagatg aaattccaga agcaccatga attattttta tttcaattag attttttttg
     2761 gctcttacat aaagttctgt gaaagtggtt gccataaaag gatcgccata agttaatatt
     2821 gctacactat catcttttga ttgagagata atagctcttg catcttcaat aaaccaacga
     2881 ggaattattt gtgtacgttt attatcagta tttagtaaaa gttctagttg cctgatatcg
     2941 tcatcatcaa tataacttgt aaatctttct atatagataa acttacattt ttgtagagtc
     3001 tcttttgcta acagagttaa tccagaataa ccatttattc caagtccaac gaaacataac
     3061 ataccggtat ttaattcgct attattacct ctaattttat ttattctgaa tgttatgcat
     3121 tctggacata tgaaataaag ctcttttgta gacttcaatt cctaaaaggt attcttctat
     3181 acttacatgt tcattaatac tatgagaaga atgcggatct ccaggaccgt atgttactac
     3241 gggaatttta aaagcatttc caatgatatt catatcacct gttcctgttt ttcttaataa
     3301 tgcaggtctt ttcttacaga tgtcaagaat agagagaaca agagacctaa ctaatggaga
     3361 ggaaaaatca gcttcgaatg gttccgtaaa atcttcaata ataaaattag tttttattcc
     3421 attttctttc gatatttttg caatgagagt ttttatagaa taaaaaactt gtttacaagt
     3481 ggtcccattt ggaattctaa tatccatagt tattttacac ttttgaggag tgacattgtg
     3541 actagatcca cctaaaattt ctgtaagaca acaactaaca gagttacttc ttttttcttt
     3601 ctcataattc gtatgtaaag tactttttat cgcattccaa aaattataca cttcctcaat
     3661 agagtttttt gctaaccaag gcgcacttgc atgtgcactg tctaaaacat cgcatattaa
     3721 tttaagagct aaccttcctt tatatgaaat tgtaatattt tctataccac taggttcacc
     3781 aaatatggca taatctatat ccaacttatt tttaacaagc tgcttactac ctgttgcatt
     3841 tccttcctca tcaactacac cagcaaatat tatagtacca ttttgtttag ggaattcaga
     3901 ggcagccaaa agcatagaga ttaagggagc tttagcatcg gatgcacctc taccatacaa
     3961 gaagccattt tctatcctta ccggaacttt atttggaaca gtatccatat ggccacaaag
     4021 caatattctt ggataaccac ttcctttggt ggcaataatg tttccaacgc tatctatatg
     4081 agcttgttca aaacctagtt cattaacaca tttatccttt agtaaattag ctaatggacc
     4141 ttcagatcta gatggagtgt aaagattaat tgatgttttt aaaagttcta ttgcataatt
     4201 agatgagacc aatgtacatc taattaattt gatattttaa agaataatct acaatcatat
     4261 cttgaatatt gatttcagta attttgactg tatttttgaa ttctgttgta ttatttactt
     4321 catgtaccaa tagaccttct tttgaacttt ccattaaatc tactcctaca atctttccac
     4381 caacagcttg agaagattta atacaaatct cttccaattc atctgtaact ggacagtttt
     4441 cagctcgacc tcctaatgcc atattagtct tccattcatc agattcagaa tacctatata
     4501 ttgcagcaac aacaacatca cctacaacta tagctcttat atcacgaggt ggcctagaaa
     4561 caaattcttc tagataatat acatggtaaa gaggaaacat atgctctctg tcttcaaata
     4621 cagcttttgc tgcatcttta tcacgtaata atgctatcaa tctaccccag cttcctattg
     4681 taggcttaat tataacagga tatccgaaat tttctaatgc tgaaagtgca gaattgggtg
     4741 aaaaagctgt tattactttg ggtgttcgta tacctttttt ttgtagcaac atatgggtaa
     4801 ataatttgtt tccacatgta acagcagtgt ttaatggatt aattacgttt gctcctaatc
     4861 cttctaacgc tgcagtagaa tgaaggttct tgaaataact aacacatctt tgaatgatgg
     4921 tttgattagt atattcacaa gagagtgtat ttaaatctaa aacaatatct ttacaatcaa
     4981 ttttctttat ttcaattcct ttctttctgg cagcttcata aatagccttt tcttcccatc
     5041 ttatagtatc ataaagaagt gtgagagaag aagatgtatt ccttccctca gtcactgtcc
     5101 ccaatcctcg ccaactactt gagcgggttt tgtagtaaat ccaccttcaa tttttaccaa
     5161 ttcatagctt gatccacaat ctggacagga cactatttca ccttcgattg aatcttctgg
     5221 tattttgatt tcggcatcac attcctcaca ttttaccata ttttattcac tcctttttta
     5281 cattaaatgt ttcttcatta agtatctttc tttctaattt ctcatcaaga tacaattcta
     5341 atacatcacc cattctaagt tcttttaacc ccttggcaag gttttctgct tcacgtatac
     5401 tagtatctag gccgagtaaa gataacaaat aagccgcccc atttttccct gcaagttctc
     5461 caaacacaat ctttcttctg tttcccacta gacgaggttc aataatttca taggcggcag
     5521 gatttcgtaa aatcgctgca agatgagttc cagccttatg tttatatgca ctatcgccta
     5581 ctataggttt agattctgga atctccaaac ttgtatattg tgaaatagtc cttgagagat
     5641 cctttaacat atgcagtctt aaattgtttt cagatccata tatcagggtc aatgcaatgg
     5701 aagcttcggc aagcgatgga atccctgtcc tttctccaaa accatctatc gtagtatgaa
     5761 tctgatctgc cccagcttca caaccagaca atgcattagc taatgctaat ccaacatcat
     5821 tatgacaatg tacatcaaga gaactttttg aggtatctat tttatcatac accattttga
     5881 ctaaattata cataccatgt ggacgcatta tacccacggt atctggaatg cttattcttt
     5941 ccacacctaa tctattcatt tctttacaca tctctaagag aaaagaaggt tcagttctac
     6001 tagcatcctc cattgtgaag cgacatttta acccatgtga ttttatatat tcaactactt
     6061 caatggccct cctttttgcc tcatctctag aaattttcag ttttgcacta agatgaatat
     6121 cagatattcc cagataggta gctatccaag ttgaattagt tttgagagcg atatctacat
     6181 ctgattttat tgctctaaca tgagcaacaa tatcagcttt taatccttgt ttgataattg
     6241 tttttgtcgc ttccaagtga tcggaggaca ctaccggact tatctctatt tgatctactc
     6301 caaatgaatc taacatccat gcgatttgaa tcctttgttt aatagtaaaa gaaactcctg
     6361 ggtgttgttc tccttctctt aatgtagaat caagaatttt tatttttttt ttttcatggc
     6421 ctacattatt gtattgatgc gcataatagt taggatcatc tgatttaagt atcattttaa
     6481 cgatattatt acacctttcg tatgtgatat aaatgttaac gctaaaaaat gaaagtaaaa
     6541 ttctgaatat ttactgcagt aattaaaata tagatatcat gtatttttta aagttttaag
     6601 aattatcacg gtatttgtat ctgaaacgcc ttcaattttt ctaacttcat cgatataaga
     6661 attaatttca gaaatttctg gagcactgat aattgctgca atatcatatt gtccagttat
     6721 ttcataaaca acgtcaactc cttctaaatt taataattta gacgttactg tagatgtgtc
     6781 agagcttgag gcaacagaaa tcagagttat tgcgctagtt tttcctttat tattgatttc
     6841 aattgtgaat tttttaataa cgttattatc agttaaattt ttaatccttc tccttactgc
     6901 tgattcggat aaaccaatag tattagctat ttctacaaac ggttttctag catccttttg
     6961 aagaatcttt attatttttt catcgaatga atcaatatta tttaacatct tttctttcct
     7021 cttctttagt taataattta tccataatgc ttaaggtttt tgctaccttt acgtcatcca
     7081 gtaccaatgg tggcaataat cgaattatat ttctccctga gtacaacata agaaggcctt
     7141 ctttaattcc atcaaataac atatccttaa tatcaaaacg taattcaaga gctaacatta
     7201 aacctaatcc gcgtacatct ctcacaattc tatgattctc tttaagttgg aacaatccat
     7261 ttttgaaaat attcccaatt tttgcagcat tctcaacgag actttcttct ataatagatt
     7321 ctatagtagc tatacctgca gaacacgaaa gtggattacc tgcaaaagtt gaagaatgtt
     7381 ctccaacttt catagcgtct aggatttcag gtttacatag agttaaaccc attggtaatc
     7441 ctccagctat accttttgct agacacataa tatccggagt cgttgaccaa tgttcaccag
     7501 cccacattcg accagttcgt cctaatccag tttgtatttc atcaaaaatt aatactagat
     7561 cattctggtc acaaatcttt cttatttgtt gtagaacacc atctgatgga acaattattc
     7621 ccgtttcacc ttgaattggt tcaataataa ctgtcccaat agaggtatcc agtgattctt
     7681 caatttttga tggattactg taaggtacaa attttactcc ttctaacaat ggttgaaatg
     7741 attttcgata tttttcattg tgtgttacag ataatgcacc gaaagttttt ccatgatatc
     7801 ccccagtcat ggctattatt ccacttttac ctgtaaattt acgagaaaac tttagagcag
     7861 attcagtaga ctcagcaccg ctattgctaa agaaaacctt agatagtttt tttggagaga
     7921 ttttcgatat tttttctaga aattctaatc taacattgtt atagagtgac atatgacaag
     7981 taataagttt ctctgattgt tttttaattg catttactac acgatcgtta caatggccaa
     8041 ttaatgcgac accataacca cccatacaat caatgtattc ttttccagaa acatcccaaa
     8101 cggtagctcc ttttcctttt gatatattta ttggaaatct ctgataaaga tttccaaggt
     8161 aattgtcttc actcattttg ttatcaccgt acaattagta tgaaatagtg cagaagaaat
     8221 tggattctca acgtcacctg aagctataat tgcttccttg acacccatat ctaaagattc
     8281 tatacatgcc attacttttt tctccattcc atgtccaatt tttggtaaag attcctttgc
     8341 tttatcatac gtcatttctt ttatcagttc atcatttaga attaaaccat taacgttagt
     8401 gataaaaatt actttatcag cggatattcc ccctgcgata taggcagcag ctctatctcc
     8461 gtcaacattt aaaaattcag tttcttcact taaagctatt ggagaaacaa ctggcacaaa
     8521 atttccttct aacaactttt caattaatag tgtattgatc ttgtttattt ttcctgtata
     8581 acctccatca atcatcattt ttctaccttt atcattaaca actacaagct ttttctttct
     8641 taatgcttcg attgtagatc catcaattcc cgatatccct gctgcactta tcccattatt
     8701 taaaagcaat ctgacgatag ttttatttat ttttccagac ataaccatgg tatatatttc
     8761 tgcagtttcc ttgtcagtat atctactttt tacacctcca ggagaaacaa taaatttttg
     8821 ttcttttccc aatttattac caataatagt gacatcctta cctcccccat gaacaagaac
     8881 aaccttgtct tgttttaata tatttttaag gtctgaaata gttgatgaat gtaatccatc
     8941 cacaatgcta ccaccaattt taattacgat cataccggtg ttaatggagt taattttaag
     9001 ccatccattt catcaaaacc aaacatgaca ttcatatttt ggattgctga gcctgctgca
     9061 cctttcataa gattatcaga agccgagagt gcaacaactc tattgttatc ctcatcaata
     9121 tcaaaaccaa tatcacaaaa attggagcca attaagaatt ttgggtctgg aaatttatat
     9181 aatccctttt tatcttttat aaatcttata aatggttcat ttttgtaaga ttgcctatag
     9241 attttccata gatctaattc accaattgtt ttagtcaaaa aagtatgatt agttgtcagg
     9301 atacccctaa caagattaac agcatgaggg ctcatactca cttttataac ctttttagat
     9361 aatctacaaa gttcttgaat aatttcacct gtatgtctat gtttggctgg tttataaggt
     9421 cggattacac catatctcat cgcatgatgt gtgcctgcat ttgcttgagc acctgcacca
     9481 gaagagccaa ttttactatc tacaactata tgatctgtat caataagatc attttcaata
     9541 agcggtttta gtgctagtaa ggatgtcact gccatacatc caggacatga aactaatttg
     9601 gcttttttaa tttcttcacg atgaagttca ggaactccat agactgattt tgaaagtaaa
     9661 tctggaaaag gatgttccca tccataccat tttatataat cattaggatt tttcaagcgg
     9721 aaatctgcag aaagatctat aattttaata tctcgttcat ataaattttt tacaatttta
     9781 ttcgcatttc catgtggaac agaaacaaat accagatcac atgaatcggc aattttattg
     9841 atatccattg atgaaaaggt aaggtctatg aatcctttta aacttggatg agccctatgg
     9901 acatattctc caacatgttg tctagatgta accacatcaa ccgttacttt gggatgagtt
     9961 accaataatc gaagtaattc tccgcctacg tatcccgacg cacctataac tcctactttc
    10021 attgttatga actatagatc agaatattgc tgcatcttat gaattttttt atcatttgaa
    10081 gtaaagacaa atattaattg aatttctatt ttttataaac tgttaagaca tactcaatca
    10141 tagaatttgc tatatctacg tgagatactt gagatgctcc tctaaattca acattactat
    10201 taatttcatg tataacaaaa cctttatttt catcttccat aacatctaca ccgagaatac
    10261 ctcctccgac tgcattggat gccttaatta caatatcttc taactctttt gtaactggag
    10321 caagccctgt atgacctccc ctcgcaacgt tagttctcca ttcatttata gcagaatatc
    10381 gatatactgc ggtaataatt tgatctccag caacgataca tctaatatct ctagggggtc
    10441 tatttatcat ttcttggacg taatagatcc tagaaaatgc tgaatcattt tcctctctca
    10501 tttccatgat aatatttacc gtttcttcat ttcgaatagg aaaaacccct ctcccccaag
    10561 aaccaacaag tggttttaaa actaaaggaa atccaagttt atttgtcaat tctttaaaag
    10621 attcagcgct aaaagtgaaa tatgtttttg gagaaggaat ttcagattta gccaaaataa
    10681 gagatgttcg taatttatta ccacaaatct catttacatc atatttgttt attactttat
    10741 gcctcaaaaa ttcaagacaa tatgttatgt ataaacctcg ataatgactt aaacatcgtt
    10801 gaagaaaaat atcaccgtta atagtattat tttgatttct atctgtacta aaagaaatag
    10861 gtttagcatc tattaaatga gtagttattc ctctattagt agccttatcg tatagttctt
    10921 tttcctcgaa tcgtagttta tcataaatta tagaaagttc catattttca aacttattct
    10981 ccccaatctt ctcctacgga ctctgctggt ttgaaatcta ctttacctgc agaattagaa
    11041 gttacttcga attcggcccc acagtctgga caggaaacga tttcgcccac aactacatct
    11101 tcgagaatat ttaattctcc tccacagtct ttgcattcaa tcttcattct attgctcatt
    11161 tcttttattc tcttttttat ttaaattaat tgctatagaa tttgctgtaa tagattgcaa
    11221 tccccataat tcaacaaaac cttttgctag ttgttgatca aaaatagagt cagaactata
    11281 cgttgccaag tcattcttat ataaagaaaa tttagattct cttcctacaa tccgagtgga
    11341 accattttgc attcgcattt ttacttttcc tgtaactctg ttttgtgttg catctataaa
    11401 cttatccaaa tcattaagca atggatcaag ccataggcca gaataagtca tccaactcca
    11461 ttgctgatct acaatatgtt tgaaccgcaa ttcgtgagag gttaatacca ttttctcaag
    11521 atcttgatga gcttgaatta gaacaattgc tgcaggacat tcataaactt ctctagactt
    11581 tataccgata attctatctt ctatatgatc aacaatacct acaccgcatg atcctgcctt
    11641 aaaattaata taatttatca gatctataag attcatcctt ttgccatcag ctgttatagg
    11701 tagacctttt tcatattcta tttccaaata acaaacttct gaattattaa atttaataaa
    11761 tttgaaacaa tcttctgggg gttcaacaaa aggattttcg agatatccac cttctattga
    11821 ccgtccccat atattcatat caatactata tttcttaact tcagtagata tgggaatgtt
    11881 atgttctttt gcaaattgaa tttctatatc tcttgttaga ttcatatctc taattggtgc
    11941 aataattctt aaatttggat taaatgattt tatagttata tcgaatctaa cttgatcatt
    12001 tcctttacct gttgaaccat gcgctattgt tttggcattt tctttatttg atatctcaac
    12061 tactttcttt gcaataagag gccgtgctaa cgcagttccc aaaggatact ggttttcata
    12121 caagccgttg gcttttatac aatgagtgat atattcattt acgaattctt ctttagcatc
    12181 tacatagtaa tgctgaatag ctcctatttc tttagaccta ttttcgattt ctttgaaatc
    12241 atcattttgt ccacaatcta ctgttacagt aataacatca agattatgta aaagctgcaa
    12301 atattttata catactgatg tatccaatcc acccgagtat gctaaaatgc atttatcttt
    12361 aaccatcgtt agccgatgcg aatatagtta ttatatttat atttttgtat tcaataagtc
    12421 gttaaattag ttgcacatct atattttgca aacaatataa aaattttgat ataaaatgat
    12481 aaatgccctt ttatctagta tataattaaa actttgtata gaagatacac aatcgatgaa
    12541 ataaaaaata aggtcattga aacattggct aaccatgaag aaggaatgtc tagtatggaa
    12601 ttggctgatg ccattcatgt taatagaatg acaataacaa aatatcttaa cgttttatca
    12661 gcaatcggtt taataaaaat gaaaaaaatt ggttcgatta atatatggac aagagaatca
    12721 ggtgtagacg atatagaatt tcctttgaat tatattcaca tacaacagaa atttttaact
    12781 gctttgcttg gatataataa aaaagaatca agtaaaattt tacttaatat tatttattca
    12841 aatactgaag ttttaaaagt tttaaaagaa ataatcattc cctcattaaa tactataaat
    12901 gggctttata acagaggtag gttaggtaaa actgaactta tttatttgaa taacttatta
    12961 tttgaccttt taattgtaat tgaaatacat tataaaaata ttgatcataa taaaaatatt
    13021 cacaatattt ttattgcagg aaatgaagat cagatattat attcgaagat attatcatta
    13081 gtctcggaag tttcaaattg tgatactata tatcttggaa atattgggca acatatagat
    13141 cctttttttg atattgattt acaaagtcat attactagat tttggtcaca taagaaaggg
    13201 acgaaaatta tttttttatg ttgttcagat gaaagttctc taaaattctt atttagcact
    13261 tcagattata taaaaagaaa aatgactgag attataaaaa tagtattatt tgtaaatcca
    13321 gaaatattaa atttgacaaa ggatttgaaa tttgatttca ttacgtcaga tctaaattta
    13381 ttaattatat ggttagaaga attgattaaa caaattagga ctaaaattta atatacttct
    13441 tcaaaaataa tctatagaag ataatactat atatggtatt tgttacaaag tttatttaaa
    13501 aattttaatc cttgtatgat ctccacttta taatttctat ccaccatagg ttccaaacca
    13561 atgtaattat tatattgtat cttctttaat gtttttaata taggattaaa attaatatgc
    13621 cctaaccctg gcatttttct attgttatct gcaaaatgaa catgttttaa aaaagatttt
    13681 gattttttga tactatcaga aaaatttttt tcttcaatat tcatatgata agtgtccaat
    13741 aaaattgaaa aataatcatt attaaggttg gaggtaacaa atattgcatc tgcagtatta
    13801 caacaaaagg gagtagaata cctatttagt ggttctagca aaagatgtac tccataatcc
    13861 tttgaaaaat tagtaagttc tgataatacg gttactgatc ttttgagtaa tttctttttt
    13921 tctttattgg gaatcatttc gtggttttga tctattttca gatcataatc agacaatagg
    13981 caaacattaa aaatagaacc tccaaaaaaa ttacaaattt ttatacactc tttgatataa
    14041 ttttttgaat aagataggat ctgtggatcg gaagtaagga gaattggagg tatcattcct
    14101 tttgagcctg tccatttacc agttacacca agaactgaaa cgctaaaagt attgaaaagt
    14161 tctgaatttt ttcttgaata agaatttgaa ggatcaccag aaacttcaat attgcgtata
    14221 cctaattcta ctagactttt aattgtattt tctaaggatt ctatttttgt aaatgaatca
    14281 atagttattg aaaaattaac caattaaatc actatacaag tatagctata gtaaattcat
    14341 aagatatttt ccaatttgaa ctacatctaa tgaattcatt tcgtcttttt tcaagttaga
    14401 tcgaatattt gatgcataaa gtgaatagcc ttcttgtgtt gtcatattaa ctggtcttcc
    14461 atgggatgcc tttagtaagc cggtatcaaa aggaatggat ctttttttag aatcaaaaaa
    14521 taattccaaa gggtcataac caggtttcct gtggatatct accatcctag taaaagaagg
    14581 agccatattt tcatcatacc accagtaata actaaaccat ttatcacgat tagctacaac
    14641 tataatgtcg cctgctcgtt catgatcaat attaaaaaat ttcttttcca ctccggttat
    14701 tataatatca atatccgaaa ttttttttaa aatatttaca attctttctt tatcttgagg
    14761 agatttgaga taaatatttg ctatttgatg atcaactacc gcgaatgctt gactaaattc
    14821 gaaatcgacg tattctttgt ctccaatttt tctcacattt aaaaggccat tatttctcaa
    14881 aataatattt atagggattg cacagttaac attattaaaa gagtattctg aaaaaataat
    14941 aaattgtgta ttttcgtaag ttttactctt ttttgttgaa tctattattt tttctacaat
    15001 actgtctgcc aaaactagat catcagaaac ttgattactc tcttttccaa aacgctgagc
    15061 agaataatca acatgtggga tatatgtaaa cataaaattt ggtctgcttt tttctatggt
    15121 atattgagcc gcttttgtaa tccattcact tgataagcta gacgctaatg gcccccaata
    15181 cgaagaaaga tcaaattctc caatttgttt cattatttct tcataataac ccactggttt
    15241 agaataacac cacattttca tttcattttc taaatgaatg ggttttggag ttataatgat
    15301 atccgaattt gcatataaag tattttgcca aaaaagaaca gctgttttaa aattaatatt
    15361 cttacttttt ataaagtccc aaattcgtgg tacctgtact aaactattat attgttccca
    15421 aaataataca ttataagtat tccgatccat aaagccatta gaaattatac catgtctatt
    15481 gggatattct cccgacaaaa agcttgtttg tactgtagat gtgactgctg ggaaaacagg
    15541 cttcatttta gaagattcac ctttttcagc taatgcagaa atagttggta gtaatttaga
    15601 attaagatgt ttttcttcta aaccaacaat atcaataaca ataacatgtt caatcataaa
    15661 cagatttaaa caatgagtaa tttaaataat atgttaaaca tagtccagaa gctgaaaagg
    15721 tttagtgtct ttaaaataat ataaaattta ctcaagtcat atacagtctt cttgataaaa
    15781 aaataacagg aattattaat actaatgcta aaataaggct ataataaacg cctaaagtat
    15841 cggttatgaa aatgatatct agaaaaacta tcatagttat taactgagat acagattttg
    15901 gagtagttgc aattttttta aacaaaccta tatgagaata aattataaaa caagaaaata
    15961 ttatcaatgg aattaaggat tgataattaa atgcacccag aattataaag atacataaag
    16021 aaaatatgag aacatatata attatgaatg gaatattatt ccaaagagtg aagaaaaagt
    16081 ctttcgtttc attccgactt aaaatagtaa tagcacatac atataaaaat tcggataaca
    16141 atggaataat aaaacctgtt tcaaacatac ctccatttat aagaaatagt gattgactag
    16201 ctcctaaaag gacatttaag cttcttataa ttcccatgtt aatagatccc caaattgtct
    16261 tttttgaaaa tctattatac gagaaaatta aagtaaccat aattacagta attataaaag
    16321 tataccatcc cataataaac gaaataatta gagagtatga aaaagctatg aatgctaaaa
    16381 ttaaagcatt ttttttagat attttattgg agggtaaagg acgcccaggt ctttctttct
    16441 tatctatttt tatatcaaaa taatcgttta gtatcatccc tccaatatat aaaaaagcag
    16501 atattgaaat caattttaaa ataacaaaat aatcaaaata gtctatgcta gtaaagaaaa
    16561 tataacccaa tagaatgttt gaaaaagtag taaaaacatt ggggatacgt actagtctaa
    16621 aataatttat tatagtagat gacatattct taatcattaa ctcccattaa aagttacaac
    16681 aaaaagacaa aatgacatgg tgtaaaaatc tgtactattc aacaataaaa tcctacttat
    16741 tagaataatc ttttaatttc atattaaaat cagatgaaat gaagcaatga tagactagtt
    16801 ggatgatgat cttcttctgt tccgcagagg atatttacaa catgggcacc tatttccatc
    16861 atattttata taaatagcac attctgtaca tcgtttaaaa ttttgagcat ataaagagta
    16921 ttttcctcct tttttgcttc caaatctact acaatatcct ttacagctca aatatactga
    16981 atgtaatggg gattgaatta attattttga tgtttacatt gcaatcgttt gtattacatt
    17041 tcatttcact atactcataa ttttaaattt agttattcaa attgtaagta ctatgaaaga
    17101 ttgagtaaat tcctttaatt attgttggtt acctacttct cttgatttca tcagtgttat
    17161 caatatactc acgattaagg ttaaaaatat gatctcagaa aaatattgat agtgataatg
    17221 atttcaaaca cagaattttg tcattatgaa tgaaagaaga tgtcattttt ttctatactc
    17281 ttatattgat aaaattctat ataatttcaa atatggttac gtgcagatct atataagatt
    17341 aaagaagaaa tttcaaaagt aacgtgtgta aagtctataa aacctcagct tatccatact
    17401 tcaaaatatt taagcatgta aagataagtt atactatgaa aattcaagca tatgcagctc
    17461 atcaaatagc tggacaactt gaaccatttg agtatgagct tggtcctcta aaatctgacg
    17521 aagtagaagt agatgttgaa tattgtagta tatgtcacag tgacttgcat atgcttgata
    17581 atgaatgggg gataacacaa tatccatttg ttcctggaca cgaaattatt ggaagagtat
    17641 ctgcaatcgg gaatatggta gaacatttac aaattggcca atatgtagga gtaggctggc
    17701 gttctcgttc atgcttaatt tgtgaccaat gtacaagtgg ccatcacaat cgctgtttaa
    17761 aagccgagga tgtaatcgtt ggtagatatg gtggttttgc aaataaagta cgatgtcaag
    17821 ctgtatgggc ttttccattg tctgcaaata tagatattaa aactgcaggt ccattatttt
    17881 gtggaggaat aacagttttc aatccaattg ttcaaaataa tataaaacca ccaaatcatg
    17941 tagcggtagt tgggataggt ggcttgggac acttggcgat taaatttctc aaggcctggg
    18001 gttgtgaagt tacagctttt tcatccaatc ctgaaaaaga gaatgaggtc agacagtttg
    18061 gagctcatta tttcttaaat tcaaaggatc ctgagtcttt aaaactctcg gtaaatagat
    18121 ttgatatggt attagttact acaaatgttg aacttgattg gaattcatat ctaaatacac
    18181 ttcgacctgg tggtaaatta cacgttgtag gagcagtacc ctcattaaag gtggatgtat
    18241 ttccattaat ttctggggaa aagtcgatcg gcggctcacc cattggtagt ccagcaacaa
    18301 ttaatgccat gctagatttt tgtagtcgtc atgacattac ccctgttaca gaagaatttc
    18361 cactatctca agttaatgaa gccttagccc atctaaaggc tggcaaagca cgttatcgta
    18421 ttgtattaag aaatgatata aaatagataa aggtattatt gcgaaagagt agtatttcat
    18481 actgtttatt caaagttttt aacaatttca tatattttcc caataaaata tccctttgtc
    18541 agatccattg tattttaaat ctgtaatgtg ttttctgaac cctttattct atctaatttg
    18601 gtttttctat tgtacaatct ctcattttgt atccagaatg attggcttat gttgttttcg
    18661 tagagtaagt atttctactt tcgggcaacg agccaaggat tcaatggagg aggtgggatt
    18721 tgaatgctcg gttgaatcct attttttata gaattcatga atgactttaa taattgaaaa
    18781 ctaggaaata acctttacta tgtagttctg caaatacatt agtcgatttc atgatcctat
    18841 aaaaattgtc ataaaccaga ttaagaaaaa aaattgctat gacatctaac ttttctaaat
    18901 gtctcacaat cggaaagaca aggagctgat ttacaaacaa gaatagaaga attacaaata
    18961 ataaatcagt cattgagaga gaacgacaag gtaaaagaag atgcattggc taatatatct
    19021 gatcaactat tagttttaag taaaagagta caacaattgg aaagaaaaca aatggcataa
    19081 tctgtaaatt atatcgacaa aaagtaaatt gagggattta atacaataat gtatggaatg
    19141 tttgttttca gtattggacc atgctactct aagatgtaaa ggaatggttt gaccttcgaa
    19201 agttgctttg attcgtaaag tacaacttgc atttttattt gattcagtgc aagacttaca
    19261 agcatctata cattactgat attttgaatt ctgaagtatt tgttgactgt cgtctattct
    19321 agaacagtta ctataaacaa taataaaaaa tcatattttc catagaacat cgtagtagga
    19381 atttcaaaat ggaatgttgt tagagttcac ccatcattta gacaattatt catttgaatt
    19441 ttcttttcca ttccacgaac tattcagtgg tttttccatc aaattctggt gcgttttctt
    19501 ctgcttcctt tttagtatac cgtaagatcc cgtccttatg atgaggagga gcatagagcg
    19561 tatacatttt cagatctgtt tgcgtatcaa tattgatgat attgtgtttt gaaccagttg
    19621 gaattacgat tgcgtcacca tcctttattt cgtattcatt atcatcgata atgcattttc
    19681 catgaccacc ttcaatacga aagaattgat cattttcgta gtgaatttct tctccaattt
    19741 cttcttgggg tcgtagactc ataagtacaa gctgaacatg tttggaagta tacaatactg
    19801 tcctgaagtt attgttatct aaagcgtctt tttcgatatt tgttttatat cctttcatta
    19861 tctttgttct atttgagtta aatcatataa tattatataa taatattgtt cccatatcaa
    19921 aattcaaatc aattagttct tatattattt ataaaacaag attattcaat gtttatgaat
    19981 atataaacca tttaaattga gaaattattc tattatctat atataatttg ttttctatta
    20041 ttctatatgt cattacaaga ttctaatgaa aaaaaataca attgtcaatg cggagaatct
    20101 ttttgttcac tcgaagaatt aaatgaacat actgacttca taattcaaca ttttatttaa
    20161 aaatttaata tgaacagtct ttaataataa tataacaact caagtaatac aagaagcact
    20221 tactgaatac ttgtcaaaaa aagaaaggaa gaacgcataa agagactaat atataatata
    20281 accatatata ctaatttaaa atgccagatt gtgaaatgtg tggaactttt actgctaaat
    20341 cttataatgt tgatttaaga ataacatcta actttgacag agaagaagaa ccttttgaaa
    20401 gatatagtat tgtttgttca gactgtagaa ataaaatatt aaatgtattt ccgaaagtag
    20461 aagatgaatt tttttgaaag tataatataa taactctaaa tgatttgcat cttcgttttc
    20521 aagatgggta ttgtaactta tgataataca tacttattat cttgacagtc tactactaga
    20581 ttcgctggta gtattattag aaaatggata ggtagcaaaa aaaattattt gattattaat
    20641 gaaactactc aggaagatcg gtatctttgt gtaatgcttt atagacacct gcagcaatta
    20701 ttccacctaa gattggagca atccagtaaa gccagttata atcaaagttt ccagtcatta
    20761 aagcaggacc aaatgatcta gcgggattaa gcgaacctcc agttattgaa cctccaaaaa
    20821 taacattaaa tcctagtgtg aagcctattg caactcctgc cattggtgca attctgaaat
    20881 ttttagcagt agtagtcgct aatactacat ataccaaagt aaatgtcatt actgtttcta
    20941 atacaaatgc tctcataacg ttattctctg atggtaaagt tacacttgca gacattgccg
    21001 aaccaaacat caaataaact actgcagcac ctattactcc tccaattatt tggcatatta
    21061 tgtataatgc tgcttcttta tctccaatct ttcttgctac taatagtcct attgtaacag
    21121 caggattaac ttgtgcacca gatcgataac ctatagcgta tatgattgca gttaatacta
    21181 atgcatgtac taatcctatt cctattactc ctaattgtcc actatcagaa tatacagtag
    21241 cagcagatgc aatggcataa actaaaataa aagtacccac aatttctact aaatatttat
    21301 gttttacatc tgactttgtt ttatcacttt tatttgagat attcttaggt tgatcattat
    21361 ttttcacaaa ctagacgcta atttagtctt gataagtatt gatacagttg tatagtgatt
    21421 cttcatatca agttattcta atctaattaa aaaatcagtt attcaacaat aatagtaaat
    21481 ttttacctta actatataac cccacgagta gtacgactga agtgggactc gaaccttcat
    21541 ccataaaatg tgggttctgc tatctagtat taaaatattg ggttatttta aaaaaaattc
    21601 gaaccttcac taatattact tctacattct attttttggt catattaaca tcagtaattt
    21661 atctttgtct tctttttgtt gtttttcttt attagacaaa aatgaatgaa ttgtagttct
    21721 aagttcgttt aaccaaaacg gtttgtgtat gatattcttt tcaatattag gattatttgt
    21781 ctttagattc tctatgtatt ctttatttgc agcagtaata aatctatata atagagtagg
    21841 atcaatattt tgtaattctt gagattttcg attatagaga tctctcattt cgtatttagg
    21901 atcattcgct tgatgttgtt tgccaagaat aaggttttca gtttcagttt taggacaacg
    21961 aaccgaggat tcaatggagg aggtgggatt cgaacccacg aacccctaaa ggacgggatt
    22021 tatcattaat atttatcttg agtcctgcgc gtttgaccag gcttcgcgac tcctccaccc
    22081 aaactaataa tgtactgaga tattagaatt atcatattca gttataaata tataaaatta
    22141 ttatttataa taattattat agataaattg aactcagatt aatttaagaa cgaagatagt
    22201 atagtaaatc aaacaggtta cttcagggta gtattgtcaa cattcttccg cttctttgga
    22261 atagatacca ttttaagaat ttaaatcccc tttaattttt tttaaataaa gtatatttac
    22321 ccacattaac tttagtttaa tacatagata cctgaaatac ttaacacatt cataatatag
    22381 cttaatttcc ttactataga taataatgtc ttttaatagt ttattacttt aaataaatca
    22441 caatactctt aaagtcattg taatcaattt ctcttgaaat attagattac aagaataata
    22501 ctactctaac agctttaact atatatgtat caagtataaa aattcaataa aaagatatct
    22561 agaataaatc ttctatttta caaacttctt atttgtgtat atacaaatca ttctatttta
    22621 ctttaattaa ttttccaata aattaataaa cataaagatg atttcattaa tattgtcacg
    22681 atcatacaat aaaaacaata aaaaaactat attaccagaa gacttcgcta aagaggtaaa
    22741 aaatggatta gaaagaaaaa agaaaaaatt aaatccaaaa tttttttatg acaaaagagg
    22801 ttcagagctt ttcgagcaaa tttgtgtaca accagaatat tatttaacat taactgaata
    22861 taaaattata atagaaaata ttaattcttt attaaaatat tatgataaca aagatatctg
    22921 catcgtagaa ttaggaagtg gaagttcaaa gaaaacaaaa attttattaa attacttttt
    22981 aaaaaaacaa gatggaaatc tccattactt tccaattgac atttctcaag aaatgttata
    23041 caaaagctct ttaaaattgc aaagtgattt accaaaaatt atcaatcatc cgatagcatc
    23101 tgattatttt gagggaataa gaacagtaac aaaatttatt ggttctcaaa aaaatatccc
    23161 caataaaaaa ctaatattat tcttaggatc aagtttaggg aattttgaac caaaagatgc
    23221 aataatattt ttaagaaata taagagaaat gattgataaa gaagatacat tattaattgg
    23281 gttcgattta caaaagaaaa aaaacattct cgaggctgca tataatgatg gagaaggaat
    23341 tactgcgaaa tttaatttaa atattttaga gagaataaat aaagaattag atggagaatt
    23401 tgatctagag aattttggac atttagcatt ttataatcaa gtcaaaaata gaattgaaat
    23461 gcatttaatt tcaaaggtta aacaaaaagt taaagttaat aaacttaatc gagtttttga
    23521 tttcgaaaaa ggagaaacca tacttacaga aaactcttac aaatactcat tgaaatcaat
    23581 agaacaatta gctagtaaaa gtcatttaag agtagaaaga aattttttgg ataaaaatga
    23641 atggtttaat ctagcattat taaccccatt ttaaaacaat actattttgc acatcggaat
    23701 cctgaaaatt gtatccgttc atgaggcttg aagtagtttc tataactatt tcgaatctgt
    23761 cgtctagggg tagcaaacga tccgccacgt aatacttttt gatttattgc ccatttatcg
    23821 gtatattcag agaatctaga cttaaaacca ggatataatg aatattcaga acttgtccat
    23881 tcccaaacgt caccgatcat ttgatggcag ccgaaaaaac tcttcccttt ttcataggaa
    23941 ccacacaaag aaggtctcca aagaaaagat tctaacaagt tagcatgtac aggcagggga
    24001 agaatttcat cgccccatgg atatcttgtt tttcttttta aatcttcatt ccaactagct
    24061 gccttttccc attctgcttc ggtaggtaat cttttctttg cccatcttgc atatgcatca
    24121 gcttcataat agctaacgtt aattactggt tcatctggat taatagtgga tgttccaaag
    24181 aaatcttttt tcatccaaac atcgtctttt ttaaaccaat agaggggaga attccatttg
    24241 aattcttgaa caacatccca tccatctgaa agccagtatt catatctttc ataaccacct
    24301 tcttctataa agtttataaa atcactgtta gatacaggag tcaagtcaat ttcataagga
    24361 taaatatata ccttatgttc tggtaattcg ttatcatatg agaaaccatc accataaaat
    24421 cccatatcaa aaattcctcc aggtattttt accatttctt tattatgtct tttactttgt
    24481 gcttttttat caaaatgatc atttatttta aaaggacaat atttatcatt tgaatcagga
    24541 aatctttcaa aataattttg aaaatcatat accatcagtt cttgatgttg catttcatgt
    24601 tgatttgcta gctgtaaatc atagtggatt tctttagtta cagaattaaa tgcttcaatt
    24661 ttttcaagaa ataacaagac ttttttatct aaatattttc tataatggag actttctgaa
    24721 acagtaggtc gtggaaatct acctctgtca tatttaggca atattttttc aaacttttgg
    24781 tagtaagaat ttaaatagtc taggttaatg ttttctaagt ttaatttttc gccatacttt
    24841 aatagtattt tgtgaaaaaa ccatgttaca tgtcctatgt gccaattcgg aggactacca
    24901 aaaggatccg attgaataac ggcatcttca atacttaatg ttttaaaaag atcattggtt
    24961 agctttctga cttcggtaaa ttccgagata agatcttcta ctactttatt tattaccttc
    25021 gtcataggtt gtataataat attcgatgca ataaatttta ctttttttta cagattatta
    25081 ttgatttaca caatagatat aatagcaatt actgattgtt atttaggtgc gtaattcaga
    25141 cgagtaaatt tttttcttct aatagtgaaa atgtggtaaa gagtttagtt gaaaattctt
    25201 ttaagagcat agatgaagga ttatacaata taaccaatat tgattattct catgaacctc
    25261 ttaatttaaa gaagaatatt ttagatttaa aggcaaaaaa caaaatacca attatcactg
    25321 aaataaaatt ttcttcccct tcaaaaggag taattatgaa tagagagggt acagacttgt
    25381 taagcatatc aagagaaatg ataaaagctg gtgcagcggg tctttcagtc ttaacacaaa
    25441 aatatttgtt taatggatca ttaaacaact ttgttatgat tagacaggat acatcgattc
    25501 caatgttaat gaaagatatt atagtaagcg aaatacaatt ggattgcgct aaaaaaattg
    25561 gagcagatta tattttacta attatgaata tttttgataa aaatcttgct gaaggtgatt
    25621 tagataaatt tgttagatat gctcacaaaa agggcttaaa tattttattt gaagttcacc
    25681 atgaagatga atttaatgaa attctaaaat ttaatttaga aaagaatgat ttaataggaa
    25741 ttaacaatcg taatctagat aatttaaaaa ttgatttaga gataacagag aaattattaa
    25801 agaaatttga taagggaaaa aatgtaattg ttagtgaaag tggaataaat aataccaatc
    25861 aaataaaaca actgaaaaat gcaggagctg atgcattctt gattggaact agcataatgg
    25921 aaaagaataa acaaattgct ataaaaataa aagaattagt taacgtcatt taatgatttt
    25981 tactacaaga aacaacaaac gttacaatta ttatgcttgt tttgtttcat ttagtatata
    26041 atgaagagat ataactttcc gtttaatggt aaattcggta attttggcgg aaaatacatt
    26101 cctgaaacat tagttcccgc tatagaagaa ttagaagaat catataaaaa atttacaaat
    26161 gatcctgaat ttaaaaaaga attaaaggac tatttggaaa attatgcggg tagacctact
    26221 ccaatttatt tggcaaaaaa tcttacagaa tatgccggtg gagctaaaat ttatttaaaa
    26281 agggaagatc tacttcatgg aggagctcac aaaactaaca atacattggg acagtgcttg
    26341 ttagcaaaaa agatgggtaa aaaaagaatc atagctgaga ctggtgcggg tcaacatggg
    26401 gttggtactg ctcttgcctc agcagttctt ggccttaaat gtgagatcta tatgggctta
    26461 aaagatacag aaagacagaa actcaatgta tttagaatga aattattagg atctcaggta
    26521 catccagtaa aatctggaac acagacactt aaagatgcaa ttaacgaggc tttaagagat
    26581 tggataacaa atgtagaaaa tacgtattat gtaatcggct cagtagtagg ccctcaccct
    26641 tatcctatga ttgtaaggga ctttcaaagt ataatcggga aagaaataaa aaaacaaata
    26701 agaaaaatta ctggtacttt accaaatgcc attgtggcat gtgttggagg tggtagtaat
    26761 gcaattggtt cattttatcc atttataggt gaccaagaag tgtctcttta tggtattgaa
    26821 gcagaaggga gtggaataaa tacaggtaag cattctgcaa cattgtcagc tggttcaacg
    26881 ggaattctac atggaatgca gacctatctt ttacaagata aaatgggtca aataagtcct
    26941 actcatagta taagtgctgg gttagactat cccggggtag gccctgaaca ttcgttcttg
    27001 aaagagatta aaagagtcca ttataaatca tgtacagatg aggatgcaat caaggctttt
    27061 atgattcttt cagagaaaga aggaataatt cctgccctag aatcagctca tgcaataagc
    27121 tatggaatga aattagctaa aaaaatgaaa aaatcagaaa caatagtcat tacattatca
    27181 ggtagaggtg acaaggatgt tgaggtagta caaaactacc ttaacggaat tagagattaa
    27241 aatatgcaca agagtaaaag tagaccatca aaagagaata gaatagatag caaatttaat
    27301 gaattaaaga gtaagaatca gaaaggactt atttgttatg tagttgctgg ttatcccgat
    27361 ataaaaacat ctgagtatat cataacttca ttaatcaatg gaggggctga tattatagaa
    27421 atcggaatac cattttctga tcccattgca gatggctcaa cgattcaaga tgcaattcag
    27481 agttcactta tatctggtac taccccagat atgtgcttag aattagcttc aagaataaga
    27541 aaaacttttc caaatattcc tttaataatt atgacatatt caaacatttt gtacaaaaag
    27601 gggtacattc aatttgcaga aaaagctaaa gaaaatggca ttgacggtct tataattcca
    27661 gatataccaa tagaagaatc taaagaatat ctacatacag cacaaaatac aggattgtct
    27721 acaatttttt tagtatcacc taatacttca gaaaaaagat tgaaaatgat tagcagaatt
    27781 tgtacaggat ttttgtatgc tatatcaata tatggcacaa caggagaaag aaaaggcttt
    27841 gatgaatata ccatcgaatc aataaaaaga gtaaagaaag ttactgcagc agatgagctt
    27901 cctctggcag tagggtttgg aataagtaat cctcaacacg taaaatatat gattgatgct
    27961 ggagcagatg ctgtaattat cggaagtgca attataaaaa aaattaaaga aatagaggat
    28021 aaaaagttac tgtttaatac cttaaataaa tttatttatg aactaaaaaa tagttgtaga
    28081 tgataacaat attataataa taataaacta tatcagaccc ttattctaat actaaacctg
    28141 agttatcaga agcaaacaac atattattta ttgtgacttg atagtttttt gtatttctat
    28201 aaactattac ctcaagatcg tctaacattt ccgattcacg agtgccttct atccttttta
    28261 taacaaaatg aagtgattcg tgggatatta gtaattctat atatggttca atgtaataaa
    28321 aatcagaatg actggcaaga gcgcttgatt ttgattgaat aattatcatg gtatcatcat
    28381 tatcgtatgt aaagtagctt ggagaatcat ctatagaaca agccagtaca aaatcaccaa
    28441 cagacattaa attatcatta taattctaat agattatttt atacgtctat cgcttagaaa
    28501 tctaatctaa atatcacata tatattttat aagaaattaa tgcagcagca gcagacgcct
    28561 ttaatatatt taaagtgtaa aatattctgc ttctggatga tgaataaaaa tggcagcagt
    28621 tga
//
LOCUS       DASVEV010000135         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_631649, whole genome shotgun sequence.
ACCESSION   DASVEV010000135 DASVEV010000000
VERSION     DASVEV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631649"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VF222_06105"
     CDS             complement(<1..711)
                     /locus_tag="VF222_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142031.1"
                     /translation="MSSEEKTSHRLTLKYFIFSMVIAIIIANVIVIFSEEYSNKHSVS
                     LWTLNITAATASILCIIVIGKYGIHGLHGKSYLFLALGVISWFSADFTLLYNYYALGL
                     EEQRLVTITDALWFAGYGFLALHLFIVIRSLHIKIKSKLVIIISIITVIFISYNLVTI
                     LSYDFHTDDDFIAIIVTILYPILDFILIVPSSIILVTLRKDYEYNITWSLFSLSLLVN
                     SIADDGYVKDFVTGNSENL"
     gene            complement(1226..1426)
                     /locus_tag="VF222_06110"
     CDS             complement(1226..1426)
                     /locus_tag="VF222_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142032.1"
                     /translation="MKILLFLITTNIVIMMLSVFIDLAFAEPNDVVVIEKPKFCNSAN
                     LSYYYTLEKFQEEYKYPCVTYM"
     gene            1689..>2510
                     /locus_tag="VF222_06115"
     CDS             1689..>2510
                     /locus_tag="VF222_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7142033.1"
                     /translation="MVNTNRIISSTTIVVALIAIIMVVTSSRGGGQFFIFSDIVYGQS
                     STNTTTKGNTSNNSTTLPVLLIHGYMEDAAVWNKWVDLLKKDGISAYPITFKQSDDKC
                     GSSAEHAKELSNIIGQIKDETGKNKVNIVGHSKGGLDARVYLANNTKDVVNLIMIGTP
                     NAGSPLAESSEICSPAVYDLRPGAAATEVKMNPNTKYYTIAGDWNPKSGNCQLTLALP
                     MEESSSSTLPKPNDGIVPLSSVESQDYFINLGHSKSCHSNLMSEYEYGLAKDVIVE"
BASE COUNT          961 a          363 c          349 g          837 t
ORIGIN      
        1 tagattttca gaattgccag ttacaaaatc tttaacatat ccatcatctg ctattgaatt
       61 aaccagtaat gataatgaaa aaagagacca tgtgatatta tactcataat ctttacgtaa
      121 agttaccagt attattgaag aaggtactat taatataaaa tctaaaatag gatacaaaat
      181 ggttactatg attgctataa aatcatcgtc agtatgaaaa tcataagaaa gaatagtaac
      241 taaattataa ctaataaaaa tcactgtaat aattgatatt ataataacaa gtttagattt
      301 tattttaatg tgtaaagaac gtattactat aaataaatgt aaagcaagaa aaccatatcc
      361 cgcaaaccat aaagcatctg taatagttac aagtctttgt tcttctaatc ccaatgcata
      421 ataattatat aaaagtgtaa aatctgcaga aaaccatgat ataacaccta gagcaagaaa
      481 cagatatgat ttaccatgaa gtccatgaat tccatattta ccgataacaa taatacataa
      541 aattgaagca gtagctgcag ttatgtttaa tgtccaaaga gaaacagaat gcttattact
      601 gtattcttct gaaaatatta caataacatt tgcaatgatt atagctatta ccattgagaa
      661 aataaaatat tttagtgtta atctatgact ggtcttttct tctgaagaca aaggttgttg
      721 agaatatgat attgtcttgg tattaaggat tcatttgtaa tttaattaat aatataattt
      781 ccaaatagaa attaaaagat taaaggaaaa ttaggtagtc catgtaaaaa caacaatcca
      841 atgtggtagt aatatagtat attctaaatt gtcaaaataa ttctaaaata actttctaat
      901 aataaaagct aaaatataat gtaatagtat ccctacaata gactccactt ttagaaccgc
      961 tatcggtatt actccctcct tgagatattt gagtttctat aactttctgg taataccaat
     1021 ccagttctca attaagtttc tataataaaa actaaataga tagtattatc tctacaatag
     1081 actcttattt tataaccgct attagtatta attttaacag aaataatatt atctcgttat
     1141 attttattta tcaaaatatc ctaaacaatt atttcttgtt taagtaactt caaatgaatt
     1201 taccaacgtg agaagttaca gttttttaca tatatgtaac acatgggtat ttgtattctt
     1261 cttggaattt ctccaatgta taataataac ttaaatttgc agagttacaa aactttggtt
     1321 tttcgataac aacaacatca tttggctcag caaatgctaa atctataaaa acagataaca
     1381 tcatgattac aatatttgtt gtgattaaaa ataacaggat tttcacttca acactattaa
     1441 gctaaagatt attataaatt ttcaaattct tatttcataa tgagataact acacatatat
     1501 taaatcctac tgagcttatt cattcatatt ttaattcatt tcatataatt tatcaaatac
     1561 taaaaagcta aatagtatgt aatagaaccc tactacgtgg actgtttcat acctttcttt
     1621 taatattata tattaaaaac ttgctttgga cttgtaatac taataataga taataccact
     1681 aaatattaat ggtaaatact aatagaatta tttcatctac tactattgtt gttgctttaa
     1741 tcgcaattat aatggtagtt actagtagta gaggtggcgg acagttcttt atttttagtg
     1801 atatagtata tggtcagtct agtacaaata ctactacaaa aggtaataca tcaaacaatt
     1861 ctactacact tcctgttctt ttaatacatg gatatatgga agatgctgct gtttggaata
     1921 aatgggtaga tttactcaaa aaagatggca tttctgccta tcctataaca ttcaaacaat
     1981 cagatgataa gtgtgggtca tctgcagaac atgcaaaaga attatcaaat ataattggac
     2041 aaatcaaaga cgaaacaggg aaaaataaag tcaatattgt tggtcacagc aagggaggat
     2101 tggatgcaag agtatacttg gctaacaata caaaagacgt tgtaaatctt atcatgattg
     2161 gaacaccaaa tgcaggctct ccgctagcag agtcaagcga aatttgtagt cctgctgtat
     2221 atgatttaag acctggtgca gcagctactg aggtaaaaat gaatccaaac accaaatact
     2281 atactattgc aggtgattgg aatcctaaat caggaaactg tcagctaaca ctggccctac
     2341 ctatggagga atcaagctct tctactctgc ccaaacctaa tgatgggatt gtgcctttat
     2401 caagtgtaga atcacaagat tattttatta atcttggaca cagcaaaagt tgtcattcta
     2461 atctaatgag tgagtatgaa tatggattag caaaagacgt aattgttgaa
//
LOCUS       DASVEV010000136         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_440644, whole genome shotgun sequence.
ACCESSION   DASVEV010000136 DASVEV010000000
VERSION     DASVEV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440644"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF222_06120"
     CDS             complement(<1..300)
                     /locus_tag="VF222_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7142034.1"
                     /translation="MAVLIPAALVSFFIGITGIIFMPDEIKNKSLDFDRGTIKIDDSV
                     IDVEIAESEAERQRWLMFRNEEFPLNSAMILVYPKSDLYALWLLNIQYNLDILWFN"
     gene            complement(374..>2277)
                     /gene="acs"
                     /locus_tag="VF222_06125"
     CDS             complement(374..>2277)
                     /gene="acs"
                     /locus_tag="VF222_06125"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7142035.1"
                     /translation="KITLEKNNFQAKRAQSLQNIEAFWAEQAENLVWNRQWNKTLEWN
                     PPFAKWFIGGLLNASINCLDKHINSEVKNKAAIIWEGENGENITITYYQLYQKVNQFS
                     NALKNLGIRKGDRITIYLPMVPELPIAILACSRIGAIHNVIFSGFSSTALVDRINDAK
                     SKVIITADGGFRRGNIIELKKIVDEAIVNTPSIQNVVVLRRTGKHVEMKNKDVWWHDI
                     IQNQSHYCEPELVESTHPLFILYTSGTTGKPKGVVHSTGGYLTHIYSTAKWVFDFNQQ
                     DIFFCSADIGWITGHSYIVYAPLMHGVTEILYEGTPDYPQPDRYWSLVEKYGITILYT
                     TPTALRMYRKYGNKIPNSFNLSTLRLLGTVGESINPEVWMWYFTTIGKEKCPIVDTWW
                     QTETGGIMISHCTGIDIMPMKPGSAMFPVPGVDLAIVNDEGNTLLQNQKGYLVIKKPW
                     PGMLMTLWEDDEKYKNNYWKFQKGYYFAGDYAIMDDDHYFWLLGRSDDVIKISGHRLG
                     TIELESTFISHTSVSEAAVTSKSDKTKGESIVAFLVLKKDCIESDQLRKEIIEHIRKN
                     IGAIATPNEVYFVNKLPKTRSGKIMRRLLKSIASGLTIGDTTTLEDDTSIEEIQKIYN
                     ELNDIIKIN"
BASE COUNT          708 a          423 c          302 g          844 t
ORIGIN      
        1 attgaaccac aaaatatcaa ggttatattg aatattcaat agccataatg catagagatc
       61 agattttgga taaactaaaa tcattgcaga gtttaacgga aattcctcat ttctgaacat
      121 tagccatctt tgtctttcag cctctgattc tgctatttct acatctatta ctgaatcatc
      181 tatcttgatt gttcctctgt cgaaatccag tgatttattt ttgatctcat ctggcataaa
      241 aattatgcct gttattccaa taaaaaaaga cacaagagca gctggaatta agacagccat
      301 cttgcgaacc aactaaagta atgtaatgaa tctatgattt gaattttact tttagtaatg
      361 ataaaaaaat taattaatta atttttatta tatcgtttaa ttcgttataa attttctgaa
      421 tttcttctat tgatgtatca tcctctagag tcgtagtatc tcctatagtt agaccgctag
      481 ctatagattt taacaatcgt ctcattattt ttccacttct tgtttttggt aacttattta
      541 caaagtatac ctcattagga gtagctatag ctcctatatt ttttctaata tgctcaatta
      601 tttctttacg taattgatct gattcaatac aatctttctt tagtactagg aatgctacaa
      661 tagattcacc ttttgtctta tcagatttac tagtgactgc tgcctccgat acagacgtat
      721 gtgatataaa agtactttcc aattcaatag tacctaatct atgtccagaa attttaataa
      781 catcatctga tcgacctaaa agccaaaaat aatgatcatc atccataata gcgtaatcac
      841 cagcaaaata gtaacctttc tgaaacttcc agtaattatt tttgtacttc tcatcatctt
      901 cccataaagt cattagcata ccaggccatg gctttttaat aactaaataa cctttttgat
      961 tttgtagtaa agtatttcct tcatcattta caatggccaa gtctacacct ggtacaggaa
     1021 acattgctga tcctggtttc attggcataa tatctattcc agtacaatga ctaatcatta
     1081 ttccacctgt ttctgtttgc caccatgtat ctactatagg acatttttcc tttccaattg
     1141 ttgtaaaata ccacatccat acttctgggt taattgattc tcctaccgtt cctagtaatc
     1201 gaagagttga taaattaaat gaatttggta ttttattacc atattttcta tacattctaa
     1261 gagcagtagg tgttgtatac aaaatagtta ttccatattt ttcaacaagt gaccaatacc
     1321 tatctggttg tggataatca ggagttccct catacaatat ctcagttact ccatgcatta
     1381 agggtgcata aactatatag ctatgacctg ttatccaccc tatatctgca gaacagaaaa
     1441 aaatatcttg ttgattaaaa tcaaaaaccc attttgctgt agagtaaata tgcgtcaaat
     1501 aacctcctgt actatgaaca actccttttg gcttaccagt tgttccagat gtataaagta
     1561 taaaaagagg gtgtgtactt tctactaatt caggttcaca ataatgtgat tgattttgaa
     1621 ttatatcatg ccaccagaca tctttatttt tcatctcaac gtgctttcct gttcttcgta
     1681 atactacaac attttggatt gaaggagtgt tgacaatagc ttcatcaaca atttttttta
     1741 attcaattat atttcccctt ctaaatccgc catcagcagt aattatgacc ttagatttag
     1801 catcatttat ccgatccacc aatgcggtgg aactaaaacc agaaaaaatt acattgtgaa
     1861 ttgcaccaat tcttgaacaa gctaaaattg cgataggaag ttctgggacc atcggcaaat
     1921 agattgtaat tctatctcct tttcttatac caagattttt taaggcattt gaaaattgat
     1981 taactttttg ataaagctga taatatgtta ttgttatatt ttctccattc tctccttccc
     2041 atattatggc agccttattt ttgacttcag agttgatatg tttgtctaag cagtttattg
     2101 atgcgtttaa taatccacca ataaaccatt tagcaaatgg aggattccac tccaaagtct
     2161 tattccactg tctattccat actaaatttt cagcttgttc tgcccaaaat gcttcaatat
     2221 tttgtaatga ttgtgctctt ttagcttgaa aattattttt ctctaatgta attttca
//
LOCUS       DASVEV010000137        13631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1022549, whole genome shotgun sequence.
ACCESSION   DASVEV010000137 DASVEV010000000
VERSION     DASVEV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022549"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..2036
                     /locus_tag="VF222_06130"
     CDS             336..2036
                     /locus_tag="VF222_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7142036.1"
                     /translation="MELIQISNITKKISTTKKTIRHTQSICPDCNMILDAEVFERDGK
                     VYMTKTCPSHGECEELYFGSVDMYKKFSTYWKDGKGAHAPNVMVDKCACPTNCGLCTN
                     HLSHSGLSNMIVTNRCDLTCWYCFFYVKKGLEGAYLYEPNYEQIRAMMKTLKAERPIA
                     GNSIQVTGGEPMLRDDIVDVIKIMKEEGVDHVQLNTNGIKLALSPETMREVRNAGVSN
                     LYLSFDGVTPRTNPKNHWEIPYTLESARKCGMTVVFVPTVIKSINDHELGGIIRFAQK
                     NMDVVHAVNFQPVSLTGRMGKKEREKYRITIPDCIERIEEQTNGEISTDSWFPVPSCM
                     PMTDVIEAFSKKPKYELSIHFACGAGTYVFEDVQTKKLIPLTSFVDIKGLLEYFEEKA
                     DELRSGANRYWAMLDVMRKLNQFVDRSKQPHGLNLAKMFSSILLKRNFDAVGSWHVRS
                     LFLGMMHFQDKYNEDLERLQRCDIHYLTPDLRVIPFCAFNVIPEWYRDRIQKKYSIPV
                     EEWEKTHGQTLEAGLYRGLMRRGKPEAQMGCAVSEMHKAAAESEDHRAVDLRTGMAGA
                     "
     gene            2161..3021
                     /locus_tag="VF222_06135"
     CDS             2161..3021
                     /locus_tag="VF222_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7142037.1"
                     /translation="MSTMKYVLLEPMGDVAIIKINRPEALNAMNVDVIWELSKTIDIV
                     GVDDSIKTVIITGAGERSFCAGADISYMVNITPIDAEKYASSAQSVLNKIEKLEKPVI
                     AAINGFALGGGCELALVCDIRLCSNNAKIGQPEVTIGIPPGWGGTQRLLRIIGPAKAK
                     EMIFTGKMINAEEAYKIGLVNEVIQLTKEELPEAPPEGDEVKRKEYEKELAKLLNKKL
                     IEKSIFMAREITNNSYNAIKTSKYLINKGMDIDIESGLRMEIYGWALCFAHDDRKNLM
                     SAFLNKGKKK"
     gene            complement(3023..3982)
                     /locus_tag="VF222_06140"
     CDS             complement(3023..3982)
                     /locus_tag="VF222_06140"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7142038.1"
                     /translation="MNSFRALAPSSTANLGPGFDIFGLALDLFVDEIIITKKQEQNGR
                     ITISTIKQNEGMKIPFDNENNSAALVVKKMMEDYRIKDNIDIQITKGVPPGYGLGSSA
                     ASSVATAYAFDKLFDLEINLIELINYSAEGERASAGAKHYDNVASSMLGGFIIVRNFP
                     HIEFIRFEPPEDLYLVVGIPNIPVPKKKTEVARSILPLSSSLDNIINNISNASTIVAG
                     FAYKDVKMIAKGIDDKIVEPIRKKTIPAYDKVKENAINAGALALTISGAGPSVISILN
                     TDRHSHHVMKAVKEGFAESNIESKVYLCRPSKGAKHQGNVSLS"
     gene            complement(3994..4752)
                     /locus_tag="VF222_06145"
     CDS             complement(3994..4752)
                     /locus_tag="VF222_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX7142039.1"
                     /translation="MKAIYITGTAGSGKSLLTSNLLQWYIDHDNSPITLNLDPAVSEL
                     PYIPDVDIRDHIDINKIIETYGLGPNGAIIMSHDLIGTKIQDIQLEVDELNPDYILID
                     TPGQIELFAYRSSGNYFISNFQADSKVTIFIMDGVLVSSPINFVSLSLLSASINLRLK
                     TSQINVLSKRDLIIDNLENILDWAHSDTALLKALDKENNQEFSLLSKDVRKIISQNGL
                     NQELVAISNLTMNGLIELSSVLSRILNQGEEIEY"
     gene            4876..5910
                     /locus_tag="VF222_06150"
     CDS             4876..5910
                     /locus_tag="VF222_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7142040.1"
                     /translation="MNWRLIAVIVSFLPIILLFIFSPISVTDIFSVGIAPFIMAVLFS
                     LSKVFLQAYRFKYFVDKFLEYKIASSFKLISVRIGSEFVTNTTPSYIGGEIVRIAWLT
                     KNKVPAGIAAWIATMEIIADVFVGTILALFAGIIALYNGAILIGTIIILISSFIFGFW
                     LIVVIFSAHYNIQLPKFITKLVKRFLSHERSDKIINYTNSAISDLCTMSRENFNSRRT
                     IKIFFVGMVLTFFAFACLGASFLVLGGLIESDLGLYDSILATAASTELGSLPITVGGS
                     GLTEIGLWAYVSDLNNLPSINEVLNDSHLDVIISWRIASYHIPLMIMWIVLMRLTVVK
                     KIPSRTKQKV"
     gene            complement(5969..6406)
                     /locus_tag="VF222_06155"
     CDS             complement(5969..6406)
                     /locus_tag="VF222_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142041.1"
                     /translation="MPRTKSSEKSSEKVGIPQWIEEEIKSIEFTESEIINRTGYILEI
                     YLEDYKLDIQLYEALPDGRTIVEGINVPSSQDTSKFMKGFVYEFKIKMARGKLTDKTI
                     EFLKSQFTLEMDSIYQFELMELQLMDVESEENLQQSLDDTDDL"
     gene            complement(6469..7086)
                     /gene="rnhA"
                     /locus_tag="VF222_06160"
     CDS             complement(6469..7086)
                     /gene="rnhA"
                     /locus_tag="VF222_06160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF041175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX7142042.1"
                     /translation="MYDDYNVQIKKLTCTFDGMCQPVNPGGVACYGFTIQEKNNNRIY
                     QIYGDGGIAYEPFSKESSNNVAEYIALVKLLEWLLANGYNNKFYVLIQGDSQLIINQL
                     CGKYKVKSEKLKIYYEKTKTLMKRFKNLQIEWITREKNNEADALANKAYIDFLDNNYA
                     ELNTKLKPHFATDQQIKLLSDLKIKPSKYLSRIEANRFLKKYNKK"
     gene            complement(7166..7726)
                     /locus_tag="VF222_06165"
     CDS             complement(7166..7726)
                     /locus_tag="VF222_06165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7142043.1"
                     /translation="MIEYDTLNKIALDIKNCKLCFLCSTRKNTVPGEGGFLKKLMIIG
                     EAPGYHEDQSGRPFIGAAGKILRNALSKNDIKLEDVFITNIIKCRPPNNRPPYINEKK
                     ICMQYLERQIFLLQPKIICLLGNVVSSYLLDIKSITNERGRILQKNDLKYFITFHPAA
                     TIYNRNLLNIFEQDIKKLAQYISPNY"
     gene            8378..9049
                     /locus_tag="VF222_06170"
     CDS             8378..9049
                     /locus_tag="VF222_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142044.1"
                     /translation="MIIENCNFPDNLLYDPDNFVWVDVSNENFIKIGVIPILSSISGK
                     LQSIKLKKEGTYIEKGKSLGSLESPKYFGIVRSPLNGKIKEINESILSKPKQVNDSPY
                     ELGWLSTIVPSNFDNDLRGLKKIGECYIQFKSLIDEFHVRCFKAYPDYEMYELGTECA
                     ATLTKLDELLTKIDIGAIVYLASDDITADLELTRWSKERSQSILEIRKEGLIFHFLVR
                     KEKNN"
     gene            complement(9055..9897)
                     /locus_tag="VF222_06175"
     CDS             complement(9055..9897)
                     /locus_tag="VF222_06175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7142045.1"
                     /translation="MEYANPNVLVDTQWVEDNLDDNNVRIAEVDYDPKANYNLGHIPK
                     SILFDWKRDINDPLTRNILSKDDCSILLQKAGINDDTTLILYGDFNNWFAAFAFWVFE
                     YYGLNNIKIMNGGRKKWLEEDRPISKDVPSFPMGNFKPTEPNPSLRAFLRQVRESLES
                     DKTKRIDVRSPQEFSGEITAPAEYPTEHAQRGGHIPGAVNIPWAKAVNDDGTFKSADE
                     LTKLYQTAGITPEKEIITYCRIGERSSHTWFVLKHLLGYPDVKNYDGSWTEWGNMIDN
                     PIEK"
     gene            complement(10064..10564)
                     /locus_tag="VF222_06180"
     CDS             complement(10064..10564)
                     /locus_tag="VF222_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142046.1"
                     /translation="MKINNIKLKAKIDHIFGICCYHLLPIEQEYYMGRGKIIAICTLA
                     SLDLLKEIKNNNEIMEKISIVGRLLSENKGIDEIIRFTKKNTLLKYILVCGEDVKGHL
                     PGQTLISLKENGIDINKRIMKSSGAYPFLTCSTQDVESFRNQVNIINKKGLKDINKIK
                     DIVLNI"
     gene            complement(10597..11550)
                     /locus_tag="VF222_06185"
     CDS             complement(10597..11550)
                     /locus_tag="VF222_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142047.1"
                     /translation="MQNKQTILQNWSNENVPINEIIDNIFFLINLDDVKMKKNVIEIS
                     RNTFHATPSEKWFYALKIISVYSVKNNVIFSQSQLYDLLTIMSRIIDEKKKDLVNTIT
                     FNIYRDIMENYNKLNENIKLIFPVIIVNLMYIIKYGQDIEGNSVNLFAINNYYQNSKI
                     NLEDELKKILSFKLESSNSIQDIQDNKLFEILEKLTGRNGKEIQNAFQNLIEPDRQKI
                     ENVCRNYHKLQEYSELIETDGFIKIIEKEAGRKLSVSQLQHAANSIKKTKIYLENILD
                     GSFVPKGKHGINHVKHNLEYGFQLMGIIQSKRRKIIIDNNN"
     gene            11695..12231
                     /gene="cobO"
                     /locus_tag="VF222_06190"
     CDS             11695..12231
                     /gene="cobO"
                     /locus_tag="VF222_06190"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7142048.1"
                     /translation="MEAENGLVVIYTGKGKGKTTAALGIVLRAVGHGFKVLMIQFIKG
                     EWYYGELSSSKRLTPEFEMIAAGKGFIGILDDNHDFMEHRKSAEDAFILAKEKILSKK
                     YDIIILDEINYAINLKLLSTNQVLELINIRPKQTSIVLTGNYAPNEIIKVADLVTEMN
                     EIKHPYQQGIKAKRGIDY"
     gene            12293..13102
                     /locus_tag="VF222_06195"
     CDS             12293..13102
                     /locus_tag="VF222_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HEX7142049.1"
                     /translation="MEDQKNLPEEGDIVIANVKQVTNHGAYVSLEEYDNRTGFLHISE
                     IATGWIRNIERYVRANQKIVLKVIRVNKTRSEVDLSLKQVSGEEKKSKIIEVKKNEKA
                     NAFMAIIKSKANLSDEQIKQIEEKILTKYDYVYDIFEATTKKGKEVLLTLELSENVIN
                     IIEEESKKIPIPHVEIRGILEISSQKSNGVEIIKSILSTAETTKGNSKIMISYIAAPK
                     YRIVVAADNFKIAEKSINNSIEKIKSAIEKENGHFNFIRQESKKTQQQLVH"
     gene            13104..13274
                     /locus_tag="VF222_06200"
     CDS             13104..13274
                     /locus_tag="VF222_06200"
                     /inference="COORDINATES: protein motif:HMM:NF016057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030515 - snoRNA binding [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleolar RNA-binding Nop10p family protein"
                     /protein_id="HEX7142050.1"
                     /translation="MKHLLRKCLMCKIYTLKSQCSYCSSMTVDPTPAKFSPEDKYILY
                     RLPESYNKKDTN"
BASE COUNT         4663 a         1935 c         2084 g         4949 t
ORIGIN      
        1 tactacatga atatattgat aaatatccta atccagagga agcgtctaaa catacaatgg
       61 aagcttatgt tactatgcgt cactttgaag atgctgttag aaaggtaaga agccagagag
      121 atatgaaacc tggaatgaaa gcaagtcatt tacaatacgg ttaaaattat cgagataact
      181 attttattaa aaataatttt ttaaaaataa aaaattgaaa taatagaata gatattatgg
      241 atacttaacc taatcacctt aggattgata taattatatt tatatattaa aagaatttag
      301 attgatcaat taaaaggtaa aaaggaagaa tagttatgga attaatacaa atatcaaata
      361 ttacaaaaaa gatatctact actaaaaaaa ccatcagaca tactcaaagt atatgtcctg
      421 attgcaacat gattctcgat gcagaagtat tcgaaagaga tggcaaagtc tatatgacta
      481 aaacatgtcc gtcacatgga gaatgtgaag agctatattt tggatctgtg gatatgtata
      541 aaaaattcag tacgtattgg aaagatggca agggtgcaca tgctccaaat gttatggtag
      601 ataaatgtgc ttgtcctaca aattgtggtt tgtgtacaaa ccatttatcc cattctggac
      661 tgtctaatat gatagtaact aacagatgtg atttaacatg ttggtattgt tttttctacg
      721 taaaaaaagg tcttgaaggc gcatatcttt atgaaccaaa ttatgaacaa attagagcaa
      781 tgatgaaaac tttaaaagca gaaaggccca ttgcaggaaa ctcgatacaa gttactggtg
      841 gtgaaccaat gcttagagat gatattgttg acgttattaa aataatgaaa gaagagggag
      901 ttgatcatgt tcaattaaat actaatggta ttaaattagc actttctcca gaaacaatga
      961 gggaggtacg aaatgctggt gtgagtaatc tttatctatc ttttgatggt gtgactccgc
     1021 gtactaaccc taaaaatcac tgggaaattc cctatactct agaatcagca agaaaatgtg
     1081 gaatgactgt tgtttttgtt cctacggtta ttaaatcaat aaacgatcat gaattaggtg
     1141 gaataatacg atttgctcaa aagaatatgg atgtagtaca tgctgtcaac tttcaaccag
     1201 tttcacttac aggaagaatg ggcaaaaaag aacgagagaa atatagaatt actattcctg
     1261 attgcattga aagaattgaa gagcagacta atggcgaaat ttccactgat tcctggttcc
     1321 ctgttccatc ttgtatgcca atgacagatg taattgaagc atttagcaaa aaaccaaaat
     1381 atgaattatc gattcacttt gcatgtggtg ccggtacgta tgtatttgaa gacgtccaaa
     1441 ctaagaaatt gattccactt acatcatttg tagatataaa aggattatta gaatatttcg
     1501 aagaaaaagc agatgaattg cgttcaggtg caaaccgata ttgggcaatg ctagatgtaa
     1561 tgagaaaatt aaatcaattc gtagatagaa gtaaacagcc tcacggacta aatctagcaa
     1621 agatgttttc cagtattctg ctaaaacgta attttgacgc agtaggctct tggcacgtta
     1681 gatcattatt tttaggaatg atgcattttc aggataaata taatgaagat ctggaaagac
     1741 tacaaagatg tgatatacat tatttgactc ctgaccttag ggtaattcca ttctgtgctt
     1801 ttaatgttat acccgagtgg tatcgtgata ggatacaaaa gaaatatagt ataccagtag
     1861 aagagtggga aaaaacacat ggacagactt tagaggctgg attataccgc ggattaatga
     1921 gacgtggcaa accagaagca caaatgggtt gtgctgtatc agagatgcat aaagcagctg
     1981 ccgaaagtga agatcatcga gcagtagatt taagaactgg catggctgga gcttagatct
     2041 tcttttttct ttaagttaat gttctagtta ttataattat ttccaaggaa attagttatt
     2101 cttaacaagc ctcttactat ttaataaagt ttaattgatg ttagttattg gaataagaat
     2161 atgtctacaa tgaaatatgt tctcttagaa cctatgggtg atgtagcaat tattaaaatc
     2221 aacagacctg aagcattgaa tgcaatgaat gtagatgtaa tttgggagtt atctaaaaca
     2281 atcgatattg tgggagtaga tgatagtata aaaactgtca ttataactgg cgctggagaa
     2341 agatcatttt gtgcaggtgc agatatatct tatatggtta acataactcc tattgatgcg
     2401 gaaaaatatg cctcatctgc tcaatctgtg ttaaataaaa ttgaaaaatt ggaaaaaccc
     2461 gtgatagctg caattaatgg ttttgcatta ggtggaggat gtgaactggc actagtttgt
     2521 gatattagat tatgttcaaa taatgcaaaa attggtcaac ctgaagtcac gataggaata
     2581 ccaccaggat ggggaggaac acaaagattg ttacgtatta ttggtcccgc taaagcaaaa
     2641 gaaatgattt ttactggaaa aatgataaat gcagaagaag catacaagat aggattagtt
     2701 aacgaggtga ttcaattaac aaaagaagaa ttacctgaag cccctcctga gggagatgaa
     2761 gtaaaacgta aagaatacga aaaggaattg gcaaaacttt taaataaaaa attaattgaa
     2821 aagtctattt ttatggctag agaaattaca aacaatagtt ataatgctat taaaacaagt
     2881 aaatatttga ttaataaagg tatggatatt gatatagaat ccggtctgcg aatggaaata
     2941 tatggatggg ctctttgctt tgcgcatgac gatagaaaaa atctaatgtc tgcattcctt
     3001 aataaaggta aaaagaaata acttatgata acgatacgtt accttgatgt ttagctcctt
     3061 tactcggtct acataagtaa accttgcttt caatatttga ttcggcaaat ccttctttaa
     3121 cagctttcat tacgtggtga gaatgcctat ccgtattcaa tatcgaaata actgaaggac
     3181 cagcaccact aatagttaga gctagtgcac ctgcattaat agcattttcc tttaccttat
     3241 cataggctgg aatcgttttt ttcctaatag gttcaacaat tttatcatca attccctttg
     3301 ctatcatttt tacatcttta taggcaaaac ctgccacaat ggtacttgcg ttagaaatgt
     3361 tatttattat gttatccaaa gatgaactca aaggtagtat acttctggca acttctgttt
     3421 tcttttttgg aactggtatg ttgggaattc caactacaag ataaagatct tcaggtggtt
     3481 caaatctgat aaattcaata tgtgggaaat ttcttactat tataaatcct cctaacatag
     3541 aactagcaac attatcataa tgtttagctc ctgctgaagc tctttctcct tctgcagaat
     3601 aattaattaa ctcaattaaa tttatttcta gatcaaataa tttgtcaaaa gcatatgctg
     3661 tagcgactga tgatgctgca ctacttccta aaccatatcc tggaggaact cctttagtga
     3721 tttgaatatc aatattatct tttattctat aatcttccat cattttcttg acaaccaatg
     3781 ctgcagaatt attttcgtta tcaaacggga ttttcattcc ctcattttgt tttattgtag
     3841 atatagtaat tcttccgttc tgttcttgtt tcttagttat tattatttca tctacaaaga
     3901 gatccaaagc aagcccaaat atatcgaaac caggccctaa attagctgtg gatgaaggtg
     3961 cgagtgctct aaaggaattc atttcttgta catttaatat tcaatttctt ctccttgatt
     4021 aagtattctt gataaaactg aagataattc tattaaacca ttcattgtca gattagatat
     4081 agccaccaat tcttgattta atccattttg agaaataatt tttctgacat ctttgctcaa
     4141 taaagaaaat tcctgattat tttctttatc taaagctttt aataaagctg tatcagaatg
     4201 agcccaatcc aagatatttt ccaaattatc tattattaaa tctctttttg ataatacatt
     4261 tatctgtgag gtttttaatc taagattaat cgaagcagaa agaagtgata aggaaacaaa
     4321 gttaattggg cttgaaacta ggactccatc cattataaaa atagtaactt tactatctgc
     4381 ttgaaaattt gaaataaaat aatttccact agagcgatat gcaaatagtt caatttgtcc
     4441 gggagtatca attaaaatat aatctggatt taattcatca acttctaatt gaatgtcttg
     4501 aatttttgtg ccgatcaaat catgtgacat tattatagca ccatttggtc ctaacccata
     4561 agtttctata attttattaa tgtctatatg gtctcgaata tctacatctg ggatataagg
     4621 taattcagat acagccgggt ccaaattaag ggtaataggg gaattatcat ggtcaatata
     4681 ccattgtaag aggtttgaag taagtaagga ttttcctgaa ccagctgttc ctgttatata
     4741 aatggctttc atttattttt gtatttaaat ataattattt aattttttat aatttattct
     4801 aaaagtgtga ggtttaatta aattagtaaa ttgattgatt attatataac taaattatct
     4861 tagtaatcag ataagttgaa ttggagactc attgcagtaa tagtaagttt tttaccaatt
     4921 attttattat ttattttctc tcccatatct gttactgata tctttagtgt tggaatagct
     4981 ccatttatta tggctgtttt atttagtctt tcgaaagttt ttcttcaggc atatcgcttt
     5041 aaatattttg tagataaatt tttagagtat aagatagcat catcctttaa attaatctct
     5101 gtaagaatag gaagtgaatt tgttaccaat acaacacctt cttatattgg aggtgaaatt
     5161 gttagaattg cctggttaac taaaaacaaa gttccagctg gaattgcagc ctggattgct
     5221 acaatggaaa tcattgcaga tgtttttgtt ggaacaattc ttgcattatt cgcaggaata
     5281 attgcgttgt ataatggcgc aattcttatc ggtactataa ttatcctaat ttcttcattt
     5341 atttttgggt tttggttgat agttgttatt ttttcagccc attataatat tcagcttccc
     5401 aaatttataa caaaattagt gaaaagattt ttgtcacatg aacgttcaga caaaattatt
     5461 aactatacaa attctgcaat tagtgattta tgcacaatga gtagagaaaa cttcaattca
     5521 agaagaacaa tcaaaatatt ctttgttgga atggttctta cattttttgc atttgcttgt
     5581 ctcggtgcat catttcttgt acttggaggt ctcatagaat ctgatctggg actgtatgac
     5641 tctatcttgg ctactgcagc ttcaactgaa ttaggaagtt tacctataac tgtgggggga
     5701 tcaggattaa ctgaaattgg attgtgggca tacgtctcgg atctaaataa tttgccatca
     5761 attaatgagg tcttgaatga ctcgcattta gatgtaatta tttcttggcg aattgcatct
     5821 tatcatattc ctctaatgat aatgtggatt gtgttaatga gacttacagt agtaaaaaaa
     5881 attccctcaa gaactaaaca aaaggtttga acaataatcc tttttataaa aactatagag
     5941 atttttcaat tttaaattca atatttaatt ataaatcatc ggtatcgtca agtgattgtt
     6001 gtaaattttc ttcagattcc acatccatta actgtaattc catcaactca aattgatata
     6061 tagaatccat ttctaacgta aattgagatt tgaggaactc tatagtttta tcggtaagtt
     6121 ttcctcgagc cattttaatc ttgaattcgt aaacgaaacc cttcataaat tttgatgtat
     6181 cttgagagga tggtacattt atgccttcta ctatagtcct tccatcaggt aatgcttcat
     6241 aaagttgtat gtctaatttg tagtcctcca aataaatttc caaaatatag cctgttctat
     6301 ttataatttc agattctgta aattctattg atttaatctc ttcttctatc cattgtggaa
     6361 ttcctacctt ttctgaagat ttttctgaac tctttgttct tggcattcaa aatatacaat
     6421 aaacaaacta gataaatgct ttgcataaat tgaattaaat aaaaaatatc attttttatt
     6481 atatttttta agaaatctat ttgcctcaat tctacttaag tatttagagg gtttgatttt
     6541 taaatcactt aatagtttaa tttgctgatc cgttgcaaaa tgtggtttta atttggtatt
     6601 taactcagcg taattgttat ctaaaaaatc gatatatgct ttatttgcta aagcgtcagc
     6661 ctcattattt ttttctcttg ttatccattc tatttgtaga tttttaaacc ttttcattaa
     6721 tgttttagtt ttttcataat agatctttaa tttttctgac ttaactttat atttaccgca
     6781 aagttggtta ataattaatt gagaatctcc ttgtatgagt acataaaatt tgttattgta
     6841 gccgttggca agtaaccatt ctaataactt tactaatgct atatactctg caacgttatt
     6901 actcgattcc tttgaaaaag gttcataggc aatacctcca tctccataaa tttgataaat
     6961 tcgattatta tttttttctt gaatggtaaa accataacat gctactccgc caggatttac
     7021 aggttgacac attccatcaa acgtacaagt taattttttt atttgaacat tataatcatc
     7081 atacatccaa ttgctattat aattatctat tatttaataa tagatcaaaa ggatatctta
     7141 atttgagttc aataattttt ctttattaat aatttggaga tatgtattgt gcaagttttt
     7201 ttatatcttg ttcaaatata ttcaaaagat ttcgattata aattgtagcc gctggatgaa
     7261 aagtaataaa atattttaag tcgttcttct gcagaattct tcctctttca tttgtaatcg
     7321 atttgatatc aagaagatat gaactaacta catttcccaa taagcatatt atttttggtt
     7381 gtagtaaaaa gatttgtctc tctaaatatt gcatgcatat ctttttttca ttgatgtagg
     7441 gtggtctatt gtttggaggc ctgcatttta ttatatttgt aatgaataca tcttctaact
     7501 ttatgtcgtt ctttgataat gcgtttctta atatctttcc tgctgcacca ataaaaggtc
     7561 ttcctgattg atcctcatgg taacctggtg cctcacctat tatcattaat ttcttaagaa
     7621 aaccaccttc accaggaacc gtgtttttcc tagtactaca caaaaaacat agtttacaat
     7681 tttttatatc taaagctatt ttatttaggg tatcgtattc gatcatatca atagtgaata
     7741 accatattat ttatttcgat tcaaaaatta gaatattaat aacattaatt tactgctaat
     7801 aaatactatt tattcaacta aatctttatt taaatattct acaattaacc tcttttctaa
     7861 attctaatac ttactctgat ttcataacag tataatctat tccataattc tatgaaatta
     7921 ttatgccatc atcgtttttt attatttctt attataagat ataattgacc tatatctagt
     7981 aaattgattg attgatttca tgtttctttt tatatactat ttattagaat ataagtaatt
     8041 aaaccctttt ttgtttcagt gaaatttcat tgtaaatact gtataaaaat gataaaataa
     8101 atatactata attgcatcaa acaaaaattt tcttgttctg aacatttaat tagtaaataa
     8161 atcaatccac ataactttat tattattatt gttaatgaag ttatataaat cgatttgaga
     8221 tattatataa agataaattt attttagaca aaattaaact ggttacatta tcaatagtta
     8281 tattttacat gttcttaaat atatatttta tttacgatta ctaaaattat ttagaataat
     8341 gaaaagaata aaattagtta tagaatgctt ttgatttatg attattgaaa attgtaattt
     8401 tcctgataac ttattatatg atcctgataa ttttgtatgg gtagatgttt caaatgaaaa
     8461 cttcataaag ataggagtca tacctatctt atcatcaatt tcaggtaaat tacaatcgat
     8521 taaactcaaa aaagaaggta cttatattga gaaaggtaaa agtttaggct ctctagaaag
     8581 cccaaaatat tttggaatag tacgatctcc ccttaatgga aagataaaag aaattaatga
     8641 gtctatacta tctaagccca aacaagttaa tgatagtcct tatgaactcg gttggctatc
     8701 aacaattgtt ccgtcaaatt tcgataatga tcttagaggt ttaaaaaaaa ttggtgaatg
     8761 ctacattcaa tttaaatccc ttatagatga atttcatgtg cgttgtttca aggcctatcc
     8821 agattatgaa atgtatgaac ttggtacgga atgtgcggca actcttacaa aattggacga
     8881 gctattgact aaaattgata tcggcgcgat tgtttatttg gcttcagatg atatcaccgc
     8941 ggatctagaa ttgacgagat ggtctaaaga gagatctcaa tcgatattag aaataagaaa
     9001 agaaggtctc atatttcact ttctggtcag aaaagaaaaa aataattgaa ttttttattt
     9061 ttcaatcgga ttgtcaatca tatttcccca ttctgtccat gagccatcat aattttttac
     9121 atctggatac ccaaggagat gtttcaaaac aaaccatgtg tgggatgaac gttctcctat
     9181 tctacaatat gtaattattt ccttctctgg agtgataccc gcagtctgat ataattttgt
     9241 aagttcatca gcggatttga atgtaccatc atcatttact gctttagccc atggtatatt
     9301 tacagctcct ggaatgtgtc ctcctctttg ggcatgttct gtaggatatt cagcaggtgc
     9361 agttatttct ccactaaatt cttgtgggct tctaacatct atcctttttg ttttgtctga
     9421 ttctaaagat tctcttacct gacgaaggaa tgctcttaga gaaggattag gttcagtagg
     9481 cttgaaattt cccataggga aactggggac gtctttagaa attggtctat cttcctcaag
     9541 ccattttttt cgtcctccat tcatgatttt tatattgttc aagccataat attcaaaaac
     9601 ccagaatgcg aaagcggcaa accaattatt aaagtcgcca tataatatta gggtagtatc
     9661 atcatttatt ccagcttttt gtaataatat tgaacaatca tctttagata gtatatttcg
     9721 tgtaagagga tcatttatat cccttttcca atcaaataaa atactctttg gtatgtgacc
     9781 gagattataa tttgcttttg gatcatagtc gacttcagct attctgacat tattgtcatc
     9841 taaattgtct tctacccatt gtgtatctac taaaacattt ggatttgcat attccatgaa
     9901 tctaaatgag caaaacactg ttataaattt attataacta ttaagaaacc taaaccatta
     9961 gttgaataga attgtttttt cccaagagtt ctttataatt gtataataat cttttgccat
    10021 tttactaaaa cataaatcaa atatctatat cagcgaaggt aatttaaata ttaagaacta
    10081 tgtctttaat tttatttata tcctttaacc cctttttgtt gataatattt acttggtttc
    10141 taaaagattc aacgtcctga gtagaacaag ttaaaaatgg ataggcacca gacgatttca
    10201 taattctttt atttatatct attccatttt cttttaatga aattaaagtt tgtccaggta
    10261 aatgcccttt aacatcttct ccgcaaacca aaatatattt taggagtgta ttttttttgg
    10321 taaatctaat tatttcatcg atccctttat tttcagaaag taatcgtcct actattgata
    10381 ttttctccat gatctcatta ttgtttttga tttctttgag caaatccaga ctcgctagtg
    10441 tacaaatagc tattattttt cctcttccca tataatactc ttgttcaatg ggaagtaaat
    10501 gataacaaca tattccaaaa atatggtcta ttttggcttt aagcttaata ttattgattt
    10561 tcacgtacaa ttttctataa atatttaatt aactttttaa ttattgttat caataataat
    10621 ttttcttctt tttgattgaa taattcccat taattgaaaa ccatattcaa gattgtgttt
    10681 gacatgattg attccatgtt tcccttttgg cacaaatgag ccatccaaaa tattctccaa
    10741 atagattttt gtttttttga tagagtttgc tgcatgttga agctgactaa cggataactt
    10801 tctaccggct tctttctcta taatcttaat aaatccatct gtttctatta gttcactgta
    10861 ttcctgaagt ttatgatagt ttctacaaac attttctatc ttctgtctgt caggttctat
    10921 taaattttgg aaagcattct gtatttcttt tccattcctc cctgtgagtt tttctaatat
    10981 ttcaaataat ttattatcct gaatatcttg aatactgttg ctgctttcta gtttaaatga
    11041 taatattttt tttaattcat cttctaaatt gatttttgaa ttttgataat aattgttaat
    11101 agcaaataga tttacagaat taccctcaat atcttgtcca tattttatta tatacatcaa
    11161 atttacaatt ataactggaa aaataagctt tatgttttca tttagtttat tataattttc
    11221 cataatatct ctatatatgt taaatgtaat tgtatttact agatcttttt tcttttcatc
    11281 gatgattcta gacattattg ttaaaagatc atatagttga gattgtgaga aaatcacatt
    11341 atttttaaca gaataaacag atattatctt taaggcatag aaccattttt ctgatggagt
    11401 tgcatggaaa gtatttcgtg aaatctctat gacatttttt ttcattttta catcatctaa
    11461 attaattaga aaaaaaatat tatctattat ctcgttgata ggtacattct cattactcca
    11521 attttgtaat attgtttgtt tgttttgcat agctatagaa ggattaatta aattacattt
    11581 ctaaaaatat aaacttttca tacattgttc aggtaaattg acataatact attgattaat
    11641 tatatgtaat gagaaagaaa aatataattt agattttttt atgcttctat aacaatggaa
    11701 gccgagaatg gacttgttgt gatttacaca ggtaaaggaa aaggaaaaac tactgctgcg
    11761 ttagggattg tattgagagc agtaggtcat ggctttaagg ttttgatgat ccaatttata
    11821 aaaggtgaat ggtattatgg agaactttcg agctctaaaa gattaactcc tgaattcgaa
    11881 atgatagcag caggaaaagg tttcattgga atattggacg ataatcacga ttttatggag
    11941 catagaaaat ctgcagagga tgcgtttatt cttgccaagg aaaaaatatt atcaaaaaaa
    12001 tatgatatta ttatcctaga tgaaattaat tatgcaataa atctaaaact tctttcgaca
    12061 aatcaggtac ttgagttaat taatatacga ccaaaacaga catcaattgt gttaactggt
    12121 aattacgctc caaatgaaat tatcaaagtt gcagatctag tcacagaaat gaatgaaata
    12181 aaacatcctt atcaacaagg tatcaaagct aaaaggggaa ttgattacta atttataaaa
    12241 ttgagaaata cttgacaaaa taaatatagc ttgtattgga gttaaataaa agatggaaga
    12301 tcagaaaaat ctccctgaag aaggggatat tgttattgct aatgttaaac aagttaccaa
    12361 ccatggagca tatgtttctt tagaagaata tgataatagg actggatttt tacatatttc
    12421 tgaaatagca acaggttgga taagaaatat tgaaaggtat gttagagcta atcaaaaaat
    12481 tgttctaaaa gttattagag tgaataaaac ccgatcggaa gttgatttat cactaaaaca
    12541 agtatccggt gaagaaaaaa aatcaaagat tattgaggta aagaaaaacg aaaaggctaa
    12601 tgctttcatg gcaataataa aaagtaaagc taatctttct gatgaacaaa taaaacaaat
    12661 cgaagaaaaa attttaacta aatatgatta tgtttatgat atttttgaag ctacgacaaa
    12721 aaaaggaaaa gaagtattat taacactaga attatccgaa aatgtaatta atataattga
    12781 agaggaaagt aaaaagatcc ctattcctca tgttgaaata agaggaatat tggaaatttc
    12841 atcacaaaaa tccaatggtg ttgaaattat taaatctatt ttaagtaccg cagaaactac
    12901 aaaaggtaat tcaaagatta tgatttcata tattgccgcg cctaaatata gaatagttgt
    12961 tgctgctgat aattttaaaa tcgctgagaa atccattaac aattctatag aaaaaataaa
    13021 atctgcgatt gaaaaagaga atggtcactt taattttatt cgtcaagaat ctaaaaaaac
    13081 acaacaacaa ttagttcatt aaaatgaaac atttattgag aaagtgttta atgtgtaaaa
    13141 tttatactct taagagtcaa tgttcatatt gttcatctat gactgttgat ccaactccag
    13201 ctaaattttc acctgaggat aaatatattt tgtatagact tccggagtct tataataaga
    13261 aagatacaaa ttaagcaaat agatgtgaat tattgtttca ctatttttat aaattctttt
    13321 tattatgttt gtaaatatat aataattatt cccgtattct attttatgat tttctttagc
    13381 tactaggatc ttaaatttat taaattataa tatttattag ttgagtcttt ggtaaaatta
    13441 tataaagaaa aaaagagatg gaattttttt gtctagctta cttaccaata catataccac
    13501 ttggatttgc agtcatacaa ttgttttgat catatgtatt gccattggtg tttactggta
    13561 acccattagc attgtttaca tcgattgtat tcaaaagaac attgttaaag ttaacattgt
    13621 tttggttact t
//
LOCUS       DASVEV010000138         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1022584, whole genome shotgun sequence.
ACCESSION   DASVEV010000138 DASVEV010000000
VERSION     DASVEV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022584"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF222_06205"
     CDS             complement(<1..225)
                     /locus_tag="VF222_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142051.1"
                     /translation="MSQINAITKDSLALKFHHSTEQDILLAISTKGMWVTKMLFKQVE
                     ENDLVRNARKELERSRIISIQQLDSERIAIL"
     gene            398..694
                     /locus_tag="VF222_06210"
     CDS             398..694
                     /locus_tag="VF222_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142052.1"
                     /translation="MEPIDKIYYIRALLGILAGVIIAFSISPGTNQGTILGTIIITSL
                     FLYIISYWISKKIIPNVPKTEKRKFITNGIFPFLFLLVMFMIIAYTGLHQNLAT"
     gene            702..1292
                     /gene="cbiT"
                     /locus_tag="VF222_06215"
     CDS             702..1292
                     /gene="cbiT"
                     /locus_tag="VF222_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HEX7142053.1"
                     /translation="MFWNSKTPGIPDEFFLREEEVPITKEEIRSIIISKLRLKETNSV
                     IDIGCGSGSITVELCLQCQKGTVFGVDLDLQAISLTKKNLEKFKVHADIRHGNATDIL
                     ESLPFVDGIVIGGTTGQTEELIDQSISRLNSGGRLVITSILIETMFKAIKTINESNFL
                     KDIDFTQIIILKGRRTTTGTMMLARNPVMIISATKI"
     gene            1353..2078
                     /gene="cobI"
                     /locus_tag="VF222_06220"
     CDS             1353..2078
                     /gene="cobI"
                     /locus_tag="VF222_06220"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX7142054.1"
                     /translation="MKLFCVGCGPGDPELLTIKALNLIKEADVIFVPTSKSDKPSIAL
                     SIVAKYINKSTKIINLVFPMIKDKNSLKEYWKKNTSEISEMVRTGKKTLYLTVGDPSL
                     YSTWIYIHRELKNNYKDIEIEIIPGITSIFAFAAESKISLVEGDEHLSVVPACYDLNK
                     VKNTVKSSDTIVFLKDGRYFDSVIDMLSETGFSDESQIAIAQDVSTKENILEIKQLKD
                     IKGKKQPSQTYFSIMVVKRNDRK"
     gene            2065..2868
                     /gene="cobM"
                     /locus_tag="VF222_06225"
     CDS             2065..2868
                     /gene="cobM"
                     /locus_tag="VF222_06225"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX7142055.1"
                     /translation="MIESNTVYFVGSGPGDPELITIKAKKLLEKADLVIYSGSLLNPE
                     ILQYAKKEVKLYDAALLNRDEIFTLLKDITKQGKLAIRFHDGDPALFSTIREQIDKFE
                     KEGINCKVVPGISATFGAASSMNLELTLPGITQTLIITRAEFRTPVPEIERISELAKH
                     GSTMIFYLSVHLIKEIVEELLKGGRYTLDTPAAVVYRATWKDEKIIKGKLKDIAEKTK
                     KEKIIKTALIIVGNVLEPIEYQLSKVYDKEFTHGYRRGKKITNNNNNKD"
     gene            complement(2911..3573)
                     /locus_tag="VF222_06230"
     CDS             complement(2911..3573)
                     /locus_tag="VF222_06230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX7142056.1"
                     /translation="MQKKESKRASKISLRANIHGFFLTVPLDSNEMTLQNFLQDKQNW
                     ITKTYDYYRKFNPNFGKKYDDKFHIYYLGEKYSVDIVKDVVNNTIISSSINKITFHVL
                     NKKNYKRYIKNWYLKQTSMVINERIKIFSPLMNVRFANIKIKDNSTRWGSCSSKGNLN
                     FSLYLVAFPMDVIDYVVVHELAHLKEFNHSKKFWEIVRNIDHDYKEKISFLKKYGNFV
                     VL"
BASE COUNT         1387 a          552 c          594 g         1293 t
ORIGIN      
        1 caaaatagct attctttctg aatccaattg ttgaatggat attatgcgtg atctttcaag
       61 ttcttttctt gcatttctaa ctaaatcgtt ttcttctact tgtttgaata acattttagt
      121 aacccacatt cctttggtag agattgccaa aagaatatcc tgttcagttg aatgatgaaa
      181 ttttaatgct agagaatctt ttgtgattgc atttatttgg ctcaagtagt agccaccgct
      241 tgtcagcctc aaattgacat aattaacaat ataacttaac tctatggaag acaactccaa
      301 tgctaattta aaggataata gctaaatatt aactacggat ttagcaagaa tcagatcgga
      361 agaaattaaa ccccgtatta ttaattgaac ttaatatttg gaacccattg ataaaattta
      421 ctatatccga gccctattgg gaatactcgc aggtgttatt atagcattca gtatatctcc
      481 cggtactaat caaggaacta tacttggtac tattattata acgagtctgt ttttatatat
      541 tatttcatac tggatatcaa aaaaaattat tcccaatgta cctaaaacag agaaaagaaa
      601 atttattact aacggtattt ttccttttct tttcttgtta gtgatgttta tgataatcgc
      661 ctatacaggt ttacaccaaa acctcgccac gtagctggaa aatgttttgg aacagtaaaa
      721 ctcccggtat tccagatgaa ttttttctca gagaggaaga agtacctatt actaaagaag
      781 agattcgttc cataataatc agcaaactta gactaaagga aactaattca gttattgata
      841 tagggtgtgg aagtgggagt ataacagtag aactttgtct tcaatgtcag aagggaacag
      901 tctttggagt ggatttggat ttacaagcca ttagcctaac taaaaaaaat cttgaaaagt
      961 tcaaagtcca cgctgacata agacatggaa atgcaactga tatactagaa tccctacctt
     1021 ttgtagatgg tattgttata ggagggacta caggacaaac cgaggaatta attgatcaat
     1081 ccattagtag gttaaatagt ggtggacgac tagttattac ctcaatcctg attgaaacca
     1141 tgtttaaagc tattaaaaca attaatgaat caaattttct taaggatatt gattttacac
     1201 aaataataat tttaaaagga cggcgaacta caaccggtac aatgatgttg gcaagaaatc
     1261 cagtgatgat aatttccgca actaagatat aatattattt tcttttaaat gtattttatg
     1321 atttaaatta gtcagttttc aataacaatc aaatgaaact tttctgtgtt ggttgtggtc
     1381 caggggatcc agaattatta accataaaag ctttaaattt aattaaggag gctgatgtca
     1441 tttttgtgcc tacttctaag tcagataagc ctagtattgc attatctatt gttgctaaat
     1501 atattaacaa atccaccaaa attataaatc tggtctttcc tatgattaaa gacaagaatt
     1561 ctcttaaaga atattggaaa aagaatactt ctgaaatttc agaaatggtc agaactggta
     1621 aaaaaacgtt atacttaacc gtcggtgatc catcacttta cagtacatgg atttatattc
     1681 atagagagtt aaaaaataat tacaaagata ttgaaataga gatcattccc ggaattacat
     1741 ctatatttgc ttttgcggct gaatcgaaaa taagtttggt agaaggtgat gaacatttgt
     1801 cagttgttcc agcttgttat gatttaaaca aagtaaaaaa tacagtgaaa tcatctgata
     1861 ctatagtatt tttaaaagat ggaagatatt ttgatagtgt tatagatatg ttatcagaaa
     1921 caggctttag tgatgaatct cagatagcta tcgctcaaga tgtatctacc aaggaaaata
     1981 tccttgagat caaacaatta aaagatatta aaggtaagaa acaacccagc caaacatatt
     2041 tttcaattat ggtagttaaa agaaatgata gaaagtaata ctgtctactt tgtcggatct
     2101 ggtccaggcg atccagagtt aataacaatt aaagcaaaaa aactattaga aaaagctgat
     2161 cttgtaatat actcaggatc cttattgaat cctgaaattt tacagtatgc aaaaaaagaa
     2221 gtaaagttgt atgacgccgc attattaaat agagacgaaa tttttactct gctaaaagat
     2281 attactaaac aaggaaaatt agcaataaga tttcatgatg gagatcccgc cttgttcagt
     2341 actattagag aacagattga taaattcgaa aaagaaggaa ttaattgcaa ggtagttcct
     2401 ggcatctccg cgacatttgg tgctgcatct tccatgaact tagaattaac attacctgga
     2461 atcactcaaa ctttaatcat tacaagagct gaattcagga ccccagttcc agaaatagaa
     2521 agaatttctg agttggcaaa acatggaagt actatgattt tttatttaag tgttcatctt
     2581 ataaaggaaa ttgttgagga gttattaaaa ggaggaagat atactcttga tacaccagcc
     2641 gctgtggtat atcgagctac ttggaaggat gaaaaaataa taaaaggaaa attaaaagat
     2701 attgcagaga agacaaaaaa agaaaaaata attaaaactg cattaataat agtgggaaat
     2761 gttcttgagc ctatagaata tcagttatca aaagtatacg ataaagaatt tacccatgga
     2821 tatagaaggg gcaaaaaaat tactaataat aataataata aagattagtt agtgaatttg
     2881 ttataatgtt aaatgctttc ataatttctt ttacagaaca acaaagttgc catatttttt
     2941 taaaaaagaa attttttctt tatagtcatg atctatgttc ctaacgattt cccagaattt
     3001 tttagaatga ttaaattctt tcaagtgtgc gagctcatga acaacaacat aatctattac
     3061 atccattgga aaagcaacta aatataagct gaaatttaaa tttcctttac tagaacagct
     3121 cccccaacgt gtagaattat cctttatttt tatattggca aatctgacat tcatcagtgg
     3181 actaaaaatt tttattcttt catttattac catagatgtt tgtttaagat accaattttt
     3241 tatgtatctt ttataatttt ttttattcaa tacgtgaaag gtaattttat taatagaaga
     3301 agatattatc gtattattta caacatcttt aactatatct acactatatt tttctcctaa
     3361 ataataaata tgaaatttgt catcatattt ctttccaaaa tttggattaa actttctata
     3421 ataatcatat gtttttgtta tccaattttg tttatcctgt aaaaaatttt gtaaagtcat
     3481 ctcattagag tctaagggaa ccgttaaaaa aaatccatgt atatttgccc ttaaagaaat
     3541 tttagatgcc cttttagatt cttttttctg caagtaaata tattctgttt tggatactgt
     3601 aaaagatatt aacatagaga gaaaataata aaaattttaa gtcattttaa aaactcttat
     3661 tattatctaa aatgaactag tgaatctttc attccaggtt tcatatctct ctggcttctt
     3721 acctttctaa cagcatcttc aaagtgacgc atagtaacat aagcttccat tgtatgttta
     3781 gacgcttcct ctggattagg atatttatca atatattcat gtagta
//
LOCUS       DASVEV010000139        25382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_828530, whole genome shotgun sequence.
ACCESSION   DASVEV010000139 DASVEV010000000
VERSION     DASVEV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 25382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25382
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828530"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VF222_06235"
     CDS             <1..472
                     /locus_tag="VF222_06235"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7142057.1"
                     /translation="RNSEILKFKDNGEFIKKWGFEGKQNGGEFIKPHGIVLDSKNNVY
                     VTDMGNSRIQKFDSDGKFITMWGSKGLGESKFSYSIPGIGIDSSEKIYVVDRGESIIQ
                     KFDSDGKFITMWGSKGSDVGEMNKPEDIAINPSNKNAYVTDTMNSRIIEFTLKN"
     gene            complement(938..1633)
                     /locus_tag="VF222_06240"
     CDS             complement(938..1633)
                     /locus_tag="VF222_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142058.1"
                     /translation="MQQIRIGIIVLATLIALVLFSFLITNTILNSSDVFVTIFQEQNS
                     NSKIKDAKEDTKLVALHNARVPEIQDYHDILSAEVNKVNDNKILFSMELAGDANTNEK
                     YETVYLWLIYINTIDGISAPTFDKEFQLYTLIIPNFGADSNFRNKAGWYLAIFNNTNN
                     FYTLPLSKISDMPTNKVQVFIDPIFIGNPSHFNYLVSSMIRVNNTFLGKSPDYLVDFA
                     PDNYTSFWQQWFR"
     gene            2683..3063
                     /locus_tag="VF222_06245"
     CDS             2683..3063
                     /locus_tag="VF222_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142059.1"
                     /translation="MDSDIDWNDVIKKEARGNNDEDFGEVQDIQGNYILIQRGTTNKE
                     EKFYIPKDRAESYDGIILKFKFSEQELSNYQDEPPLQDTEINTNKMEEEIIVQDADFK
                     NKAKAVKNKKKETDESHNPLTIEK"
     gene            3421..3786
                     /locus_tag="VF222_06250"
     CDS             3421..3786
                     /locus_tag="VF222_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142060.1"
                     /translation="MKISELCKMIEDSIHSGKYPLQDQQQKHFMNLVEVINRSDSEDL
                     KSSDIKIEIRIQNLYTINNYVPNIQHLPGVIEIDILDSFKMLCRRLEKVVVDIPGNNY
                     SKDMTMSLDRLHKNKDKNG"
     gene            complement(3876..4697)
                     /locus_tag="VF222_06255"
     CDS             complement(3876..4697)
                     /locus_tag="VF222_06255"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142061.1"
                     /translation="MYINLEEGFKIRYDEYGKDKQKHILFIHGLGSSPIVWRDIPQAL
                     SEHFHTIAVDLIGFGESDKPELNYTISYFALFIKNFLRQIGITDQDKITIIGHSLGGY
                     IAVEYATENKEHIEKLVLIDSSGMLDGPTPLLEQYLDAAMETDPNLRYIKVKKVFENL
                     LAIPSRLLPIIVDIFISVIEKPGAKHAFESAFRNSTTTSLNLEKLKQIKNIPCLIIWG
                     EKDNLIPIDHIHKFGQILKDAELLVIDDAGHSPFIEKTAIVYQKLLTFLFNNKFN"
     gene            complement(5965..6564)
                     /locus_tag="VF222_06260"
     CDS             complement(5965..6564)
                     /locus_tag="VF222_06260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7142062.1"
                     /translation="MYRKKDSNSTLAKIAIIVGSVRQNRQGIKVARWMEEKLKNREHI
                     VYFIDPLELNLPLLDKMYKEIINPSENLKQLRNQIVEADGYMPITPEYNHSTSAAMKN
                     TLDYFLEEYYFKPSAIVSYSVGGFGGVNAVQHLRLIFAELGAPSISSSFSISKVQDVF
                     DDDGRLRDQLYEKRVVRFIEEFEWYIEAFKKQRTKGTPY"
     gene            complement(7114..8019)
                     /locus_tag="VF222_06265"
     CDS             complement(7114..8019)
                     /locus_tag="VF222_06265"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX7142063.1"
                     /translation="MTINSWPALPLEEWKNTYATLHMWTQIVGKIRLALAPPRNHWWN
                     TTLYVTPRGLTTSSMPYNDRLLQIDFDFIDHMLVIETNEGSIKRIPLRPRSVAEFYKE
                     TMAALQLLGMPVTIWTTPVEVPDRTPFEEDFKHVDYDPEYAQRVWRILAQGSRILSEF
                     SSRFIGKVSPVHFFWGAFDLAVTRFSGRTAPLHPGAPNMARFVAVEAYSHEVSSCGFW
                     PGGGPIDEPVFYAYAYPEPQGFKDYPIQPPEAFYHTGMGEFLLPYDVVRTSDTPDKVL
                     LSFLQSSYEAEAMCAKWDRHTLERQ"
     gene            complement(8326..8787)
                     /locus_tag="VF222_06270"
     CDS             complement(8326..8787)
                     /locus_tag="VF222_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX7142064.1"
                     /translation="MSVIDEDKEKKMVLSEPIIKIIKDKNFAFLGTIMRDGRPQVSPI
                     WIDIDDDNNIILINTAQGRIKQKNISRDPRVSLSLVDKNNPYSMVTIQGTVIEQTTLG
                     ANEHIDKLAKKYLNIDRYPSHSSSIQRVICKIKPEKMYYLPPRYTEFIGKS"
     gene            complement(9234..9902)
                     /locus_tag="VF222_06275"
     CDS             complement(9234..9902)
                     /locus_tag="VF222_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142065.1"
                     /translation="MIKSNDFGFNHIFINSFSNGSNNKKYDDRNKKLTLVLLHGTGGN
                     EEDLIFLGKKIEPNASILSPRGKVLENGMPRFFRRLSEGVFDLEDLRIRSHELADFIQ
                     KCSLHYKFDLENTIAIGFSNGANMSVSMLFLRPEVLQGAILFRAMVPFIPDPLPNLSS
                     KKILLSAGLEDPIVSRNETENLFRLFQKTNAIITLKWQPSSHNLIQEDILVAKPWISY
                     NFPA"
     gene            complement(9899..10900)
                     /locus_tag="VF222_06280"
     CDS             complement(9899..10900)
                     /locus_tag="VF222_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7142066.1"
                     /translation="MNNKNQISGLHHVTAICSNPQKNIDFYTTLLGLRLVKVTVNFDD
                     PTTYHLYYGDEVGHPGTILTFFPWSDAPKGHRGTGQAITTSFLIPEKSIDYWKDRLKK
                     NDICFGGPKKRFDDEQVLTLYDPDGLELELVAHSSAKENDERFWKDGPISYENGIRGF
                     YSVSLSEEGYELTSEILNELGYKMIENEGNRFRFQQVDSKSTGAKVLDVLCLPYSYPG
                     MIGIGTVHHIAFSTSSDENQKMIRKNIIKKGLNPTPVIDRTYFHSVYFRELGGILFEI
                     ATDPPGFTIDQKIEDLGSRLVLPTWLEPVRESLEKVLPKIELSKNNKDNSIISDQQQ"
     gene            complement(11100..11231)
                     /locus_tag="VF222_06285"
     CDS             complement(11100..11231)
                     /locus_tag="VF222_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142067.1"
                     /translation="MTKITLDELKKGEDSSNLSTFQPNIQERWIYSVGSYDDDGGEN"
     gene            11506..11946
                     /locus_tag="VF222_06290"
     CDS             11506..11946
                     /locus_tag="VF222_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142068.1"
                     /translation="MKVEKTTSSCFEMHFDSKTEELKTNRTHNHPKNYYTLRYFYNSN
                     KTFFHLFMHTVVYDKITNENIEKQGILLRSWNPITKRGERYVFKKGEEGDGDLNLKKV
                     YLPNTKTIFKFSKSEDIKEESYKLAEEIISVCPHKDIELKSSND"
     gene            complement(12410..12979)
                     /locus_tag="VF222_06295"
     CDS             complement(12410..12979)
                     /locus_tag="VF222_06295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7142069.1"
                     /translation="MERINWKGNEIILDAGCGSGRLTKILSTKIPNGKVFAIDLDSSM
                     IKLANERLGKISNVKFIQANICEIELQDKVDVVFSNAVLHWISNPRKVFGHFWQILKP
                     NGKLLIQCGGYGNFTKTLPVFNQVRKSQEFCNYFYNSKGEEMLKEPWYFAKAEDTETI
                     LKEIGFSNIEVFLEKKLLNFMIKKITFFS"
     gene            13237..13647
                     /locus_tag="VF222_06300"
     CDS             13237..13647
                     /locus_tag="VF222_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142070.1"
                     /translation="MVLKDAVNSDILIKLYRIYDSHRDAILWFLEELDVDSYEEYIQK
                     YGGSSIQRSYFIAVCGFFELSGVIISHRMIDPDIYFDMFNPTPFWNKAASAVEGMRKK
                     RPHIYENFEMLNNKRLGWTKKREYDRKKISHKIK"
     gene            complement(13921..14340)
                     /locus_tag="VF222_06305"
     CDS             complement(13921..14340)
                     /locus_tag="VF222_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7142071.1"
                     /translation="MSKQNKDNLNDPHIRKLFEDKNFVFLSSLMKDGSPHVTPTWTDI
                     ENGNILINTAIGRIKQKNISRDPRVALAISDQNNPYDMVTIRGKVIEQISGDAAEEHI
                     DKLAKKYIGKDKYPGRSPGEKRVLLKIKPEKIFHMKQ"
     gene            complement(14947..16629)
                     /locus_tag="VF222_06310"
     CDS             complement(14947..16629)
                     /locus_tag="VF222_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7142072.1"
                     /translation="MDKKNLAIGSTVSIGNYLIERLFNHGIRHVFGVPGDFVLGFFNQ
                     LMNSKIKVINMCDEQGAGFAADAYARINGLGVACVTYCVGGLKIVNPTAEAFAEKSPL
                     VIISGSPGIKERRKDPLLHHKVKNFDTQFKVFEEITVASVLIDNPPTAAQEIDRMLNS
                     ALSYKRPVYIELPRDIVSVDIFVDDSSNNGAEFDQYQEKSDPIILREALNETIDMINS
                     SKRPVILAGVEIQRFGLQNELLHLIEKLKIPVSSTILSKSVISELHPLYLGVYEGAIG
                     HDSVREYVESSDCLILLGALMTDIDLGGFTAKIDQRRTICINSEKFSVRYHNYEDISL
                     EDFIINLIESQDIKQRVNIINIPQSHNLQKFYAIKGQKITINRMFQCINSFLKDDMIV
                     LADIGDALFGGTDLVIHRKTEFLSPAYYASMGFAVPASIGTQLANPKLRPIVIVGDGA
                     FQMTGMEISTALRFNLNPIVIVLNNGGYGTERSILDGSFNDILTWQYSLIPQVVGGGK
                     GFIVETEEQFIDALLCAKSNTESFSILDIHLDSNDKSPALRRLAETLKKTAT"
     gene            17175..17474
                     /locus_tag="VF222_06315"
     CDS             17175..17474
                     /locus_tag="VF222_06315"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX7142073.1"
                     /translation="MVKNTGKEKEEGKRSKNDNSDTKEEDLSEGTKKRIDELPSHAQE
                     IFKEAHSSALEQYKDPDKRRGGKKESAEEVAHKVAWSAVKKKYKKDKDDEWVKKK"
     gene            complement(17596..18996)
                     /locus_tag="VF222_06320"
     CDS             complement(17596..18996)
                     /locus_tag="VF222_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7142074.1"
                     /translation="MKKFDLIVIGSGSGLDVANAAATEKGLKVAIVEKNKLGGTCLNT
                     GCIPSKLLIHSADIIEIIKKADTFGITVRDYSIDFQKIINRVNKIVDRDANEIKKGLQ
                     ISKNPRLFLQECRFIGEKNILIANDEVITSDTILIASGTRPNIPNIEGLKNVEYITSN
                     EALSLKQQPKTLTIIGGGYIACELAHFFGSLGTKINIIQRNDLLIPQEDTDISTRISD
                     IFSKRYNIYLGYNAEEVSYDSKETNGKNKTEIINVIAKNKFGDSVKLSSDQLLIATGR
                     IPNSDMLNIEKSGVKINEKGFIVVDEYLETNIKGIFALGDAVGKYQFKHNANNEAQYA
                     YNNIVHPDKKILVNYTAMPHAIFTNPQIAGVGYTEQQLTKEKIEYEKSIYPYIQTGMG
                     QAIEDKEGFVKFLIDKRNRTILGCHIMGTDAAILIHEVLVAMRMDKDEGKIDNITRTI
                     HIHPSLSEVISKAAYI"
     gene            complement(19247..19450)
                     /locus_tag="VF222_06325"
     CDS             complement(19247..19450)
                     /locus_tag="VF222_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142075.1"
                     /translation="MFYHYNDYRFQKIIDEFILSSTDDEHLSYAIRSIDYHARKLGIS
                     FYHMMFTLIQKDSSENKRKEMVK"
     gene            complement(19591..20298)
                     /locus_tag="VF222_06330"
     CDS             complement(19591..20298)
                     /locus_tag="VF222_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7142076.1"
                     /translation="MNNNNIDYSIILTSYKFDMERPSTSQIIDILKNKDYKLGIVAGF
                     TIDNHNDEYFRKCREWLKDGLIKGIKLYCGYEYYYPYDKRYQRIYDICIEHKVPVMIH
                     TGDTFSNISKVRFAHPLNIDDIAVDNPELTIIMCHLGNPWILDCQEILYKNKNVYADI
                     SGLVIGNFKLYDQRYYMYKIRELLCYLDMPHRLLYGSDWPISNMDSYIKFVDKLKLNK
                     QSRDLLLFKNSHNIFRL"
     gene            20743..21393
                     /locus_tag="VF222_06335"
     CDS             20743..21393
                     /locus_tag="VF222_06335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7142077.1"
                     /translation="MTELLHPHHTGVKVRNTIKSDIPKIIDLQKQSFPFLAKYGNIWK
                     TEELESHLDIFPEGQFVTVEPDGTIVGSASTLIVSFDPEYKEHTWMDITANGMFTNHS
                     YDGDSLYGADISSHPKYRHEGIGSMLYDTRKDLTMKMNLRRMIAGGRLFNYCEYAGKM
                     SALEYAEKVIRRELRDPVLSFELDNGFKFIKILPDYFDDVRSLNYASFIEWLNPKI"
     gene            21452..21712
                     /locus_tag="VF222_06340"
     CDS             21452..21712
                     /locus_tag="VF222_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142078.1"
                     /translation="MKLESEGIKAIKEIFTNGLYANNNIKIQRVLENLRSKDDDNIES
                     NNNSNNNSNISSSSSDNNEMSTGLNEDEEELTDEDIDNNKKF"
     gene            complement(21932..22546)
                     /locus_tag="VF222_06345"
     CDS             complement(21932..22546)
                     /locus_tag="VF222_06345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7142079.1"
                     /translation="MLDIKSSQHYVNSILTNRLLINDHNVIVINTKNSLIAGAFGINS
                     TIWPINTKGIILINSDYLDQGQFTEKEIQFILAHETAHIYKNHSLNTLMWNIIERLLK
                     NLDYNAPLIVETFKGLLTLLSPNNLPPNAEDLKNNEYEADMIALNYVTGDLRSAISCL
                     QKLSNGDLNSPSHTWELFDLKLPAMTLRQRIDVLKERYYSFRTL"
     gene            23058..23978
                     /locus_tag="VF222_06350"
     CDS             23058..23978
                     /locus_tag="VF222_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX7142080.1"
                     /translation="MFVSIQQQAFTETAESIWSIGKSMPTPRTEVTATIIDDNIYVIG
                     GFDKSGTVLDTVEVYNIKNDSWKTIAPLPQPVHHAAVTTFNGSIYVIGGYTNNNWLPS
                     AKLYIYDTKNDTWIEGSSMPTARGALTAVFIDGILYAIGGEGENRIMDTNEAYNSKTK
                     TWIAKSSMPTARHHAASAVVDGNVYVIGGRIEGSLPITNVNVNEKYDVKTDIWTTLDP
                     MPSERSGISAGSINNTIYVFGGEDITKTYNNNEKYNIKDNKWESKEPLPTARHGLSAV
                     SVNDKIYVIGGGPEPGLNVTNVNEIFNTRE"
     gene            24313..24447
                     /locus_tag="VF222_06355"
     CDS             24313..24447
                     /locus_tag="VF222_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142081.1"
                     /translation="MLKTTISDLGYADYSEWFIGHAGSTYWRKKDNEKAEIFKKIEQW
                     "
     gene            24958..>25382
                     /locus_tag="VF222_06360"
     CDS             24958..>25382
                     /locus_tag="VF222_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142082.1"
                     /translation="MKYSDDWKFDKDKQTEIGPELANLLNKEVDKFVNPDFNHFHLFQ
                     LVPYIENDGYESISDGNTVFSVSLYTNMSSLEDYKVYQNMTGLADTRVYTNLSSLGDK
                     VFPNMSSLEDYKVYQNMTGLADTRVYTNLSSLGDKVFPN"
BASE COUNT         8955 a         3709 c         3661 g         9057 t
ORIGIN      
        1 aagaaactct gaaatcttaa aatttaaaga taatggtgaa tttataaaaa aatggggttt
       61 cgaagggaaa caaaatggtg gcgagtttat aaaacctcat ggaattgttt tggattcaaa
      121 aaataatgta tatgtaacag atatgggaaa ttcacgaatt caaaaatttg atagtgatgg
      181 taagtttata acaatgtggg gttctaaagg tttaggagaa agtaaattct cctatagtat
      241 accaggtatt ggaatagata gttctgaaaa aatatatgtt gttgatagag gtgaatccat
      301 aattcaaaaa tttgatagtg atggtaagtt tataacaatg tggggttcta aaggttcaga
      361 tgtaggggaa atgaacaaac ctgaagatat agcaataaat cctagcaata agaatgcata
      421 tgtaacagat acaatgaatt caagaattat tgaatttact ttaaaaaatt gatataatct
      481 aaatttagaa tattaatagt aattaatata aaattacttt tttaattgaa gtagatttat
      541 cttttttctt aaatagaatg ttgatgttgt tctggattta ttttccctct attttctaat
      601 cttaaaattg tataaacccc agtttactac ttccaaatat ataccatgct cgaatacctt
      661 catttgatca cctattaagg tttactaata tgtaaacaaa attaatccaa aagtaaaatt
      721 ttgtttgata ttaggtcaaa gttagaaaaa taatcaaaaa attaaaacat tatgttacct
      781 tctcatgtta taattagaat taaatatttc atttgagaag acgtaggcac taatcgctat
      841 aatttgttta ttcacttcag ctatttttga atgaaggtga tatgactaaa taaataacat
      901 ttattttatt ttaacaagtt taatgagtaa ttcttcatta tctaaaccat tgttgccaaa
      961 atgatgtgta attatcagga gcaaaatcaa ctaaataatc tggtgactta ccaagaaatg
     1021 tattattaac tctaatcatg gacgacacga gataattaaa atgtgaagga tttccaatga
     1081 aaatgggatc tataaatact tgaaccttgt ttgtaggcat gtctgaaatt tttgataaag
     1141 gcagagtata gaaattgtta gtattattga aaattgctaa ataccaacct gctttatttc
     1201 taaagttaga atctgctcca aaatttggaa taattagtgt atataattga aattctttgt
     1261 caaaggtagg agcagatata ccatcaatag tattaatata aattaaccat agatatactg
     1321 tttcatactt ttcatttgtg ttagcatctc cagctaattc catagaaaat aaaattttat
     1381 tatcattaac tttatttact tcagcagaaa ggatatcatg ataatcttga atttcaggaa
     1441 ctcttgcatt atgcagagca acaagttttg tatcctcctt ggcatctttg attttactat
     1501 tgctattttg ttcctgaaat attgttacaa acacgtcact gctgtttaat atagtattag
     1561 ttattagaaa tgaaaataat actaatgcta ttaaagtagc caatacaatg atgccaatac
     1621 gaatttgttg cattttgatt aattagatct tattttaata cttaatttta gcgtacaatg
     1681 ggtttatagc atatgatctt gatgtatcaa acgacgtatt attgatgata tactaagaat
     1741 tatagtttct tatgtaggtt aagagtaacg taaaatccaa aaaatagttt tcaactttgt
     1801 tttcattgtt aataaataaa ggttttaaag atttaattgt aacgaactac ttttaattgt
     1861 tgtttacata gtcggattta ttatcaatta aaaggtaaag tttaatttca caaaataggt
     1921 cctgagtact tatcatactc gaaagccctc taatccttaa attttagtta cagataaaac
     1981 ctctttaagt aattataaaa ctattccata atattttaaa taaaaaaggt atattggcac
     2041 tcacgcattt tttttaatta ttattagtta ggagaccaat aataagggta gtttatatat
     2101 agaataaatt cttatttata taacctatta tataattcta tattgaagtt tatatcgatc
     2161 gtattaagtc taataatcgc tgggatattt ttaaaaatat tatcatattc ttgtaagaat
     2221 aaagatgaga agacgaagat gatgacaacg acaataaaga aaagaaagga aaaattcaca
     2281 taaactaggc gaataaaatc gtagattaga atcgtataaa caaagaatgg ctcatcaaga
     2341 cactattttg gaacatatta tctgagatag gtgaacctat gatagaacac caaaaccatc
     2401 ataggataat gttatcatag tctgatcttc tagttccctc tccaattttt tagttcaggc
     2461 tatggcaatt tattattaat ttaataaata tatcgaattc ttatatactg ttggtacact
     2521 taattgataa aagaattcat taaaaaatcg tctttgtatt cagtcataga caccattgtt
     2581 ctaatctatt gtacgatttg ggttcatcca ctattgaaga gtatgacaaa aattaattga
     2641 tttatatgga attattaaat aactgaaaat atataacaag ttatggattc tgatatcgat
     2701 tggaatgacg ttattaaaaa agaagcaaga ggtaataatg atgaagattt tggtgaagtc
     2761 caagatattc aaggtaacta tatattaatt caaagaggaa caactaacaa agaagaaaag
     2821 ttttacattc caaaagatcg agcagaaagc tatgatggta taattttaaa attcaaattc
     2881 tctgaacaag aattgagcaa ctatcaagat gaaccacctt tacaagatac tgaaattaat
     2941 actaacaaaa tggaagaaga aataatagtt caagatgcag attttaagaa caaggcaaaa
     3001 gcagtaaaaa ataaaaaaaa agaaactgac gaatcacata atccattaac aatagaaaaa
     3061 taggtatcca atttccaagg aaaatcaagt acaagtagga gttccaaaaa aaaggtcata
     3121 tgttaaagaa gagattgtag taaaaaaatt gttgttgaca aaaaaagaga taattaaaga
     3181 tcctatatcg gaagaacaag atacattaaa tatagataaa gaatagaaaa tacagagaca
     3241 agataaatat aaaaatatta tttaatttac tgatttcttt taaagtaata atatggatta
     3301 ataatattaa aagtgtagat gatatgaata tagtaagacc tacatttatt tttcattttt
     3361 taaatataga aataatgatt ggtataaatt aagcattatg aaatcattat taaaatatct
     3421 atgaaaattt cagaattatg taaaatgatt gaagactcga ttcattctgg aaaatatcca
     3481 ttacaagatc aacaacagaa acatttcatg aatttagtag aagttataaa tagatcagat
     3541 tctgaagatc tgaaaagcag tgacatcaaa atagaaatca gaatacaaaa tctatacact
     3601 ataaacaatt atgtacctaa tattcaacat ctaccaggag taattgaaat agatattttg
     3661 gattctttta aaatgttatg tagacgctta gaaaaagtag tagtagatat acctggaaat
     3721 aattattcca aggatatgac aatgtcctta gataggttac ataaaaataa agacaaaaat
     3781 ggttaaagaa cagaatataa atagctgaaa accgatccca actataatca tactcgaaac
     3841 catataagtg aaaatttagc accgaagaat tatttctaat taaacttgtt gttaaataaa
     3901 aatgtcaata acttctggta aacaatagct gttttctcaa taaatgggga atgaccggca
     3961 tcatcaatca ccagaagttc agcatctttg agaatttgtc caaacttgtg aatgtgatcg
     4021 atgggtataa gattatcttt ttcaccccat attattaagc aaggaatatt tttaatttgc
     4081 tttaacttct ccaaattaag agaagttgtt gtactatttc tgaaagcaga ctcaaaggca
     4141 tgctttgctc caggtttttc aattactgaa ataaagatat ctacaataat tggtaataat
     4201 cttgatggta ttgccaataa attttcaaac acctttttca ctttaatgta tcttaaatta
     4261 ggatcagttt ccattgctgc atcaagatat tgttctagta atggagtagg tccatccaac
     4321 atgcctgatg aatctatcaa tactagcttt tcaatatgtt ccttgttttc tgttgcatat
     4381 tctactgcta tatatcctcc taaagaatga cctattattg ttattttatc ttgatcggtt
     4441 attccaatct gtctaagaaa attttttatg aatagagcaa aatagcttat tgtataattt
     4501 aattcaggtt tatcgctttc accaaatcct attaaatcaa cagctatagt atgaaaatgt
     4561 tctgataacg cttgtggaat atctctccat acaataggag atgaaccaag accatgaata
     4621 aataggatat gcttttgttt atcttttcca tattcatcat atcttatttt aaatccttct
     4681 tctaaattga tgtacattta taaaatgaat ataataggaa atttggttaa tataattgat
     4741 tagcaagaga gagaaaatat tactttacaa aagtaacaat tcctctagac taatagttaa
     4801 attaattaat taaataaaat taaaaaatta aattgacttt tataaaagcc atagccatct
     4861 aaggagcaca tctcataaca atagaaggct atgatgaact aatttatttt ctttttcttt
     4921 ggactgtact aactttcatg atacatacta tttaatcatt agagacaaat caattatttt
     4981 attattgata atagcaaaaa gccatagccc tcgatgagcc aaggatatat agaaggctat
     5041 ggtatgtcga agttctttgg aatgactggg tttttcgaag taaatatcaa tgttaattat
     5101 attatttaat aattgaagac aatttaattg ccatagcctg aatggatagg agtagacttt
     5161 ggatagatag aatgtccaga agaggataac aacaatgaca atgccaatta caggatttgc
     5221 actagtagtt ttttactact aactgcttga taattctata atagtaataa taataatttt
     5281 gtatttatca atacatacta gtaaatctat cttgagagtt taatcaataa actattgtta
     5341 ttatataaaa taattttata tgttattagt aataaaaata aaaaaattaa ggaattatat
     5401 ctgcacataa tgcatagcat agatagttgc attctttctc attatttgaa tatagaccga
     5461 gttcgaaatg atatctctgt ggaaatgctg taatagattc tgtattctct actcgaatgt
     5521 taagatttcc ctcaagatca actggaagaa atattctaat tggacatcgt atactgaatt
     5581 ccttgtggcg cttaggatca attgggcttt gttgacctgt tggaccttag gttttgttta
     5641 gtttacagaa ttctactgat tcgacaaaga accattcata ttggcctttt tgacaaacaa
     5701 tttttgtctt ttgtaggata tttactgtaa ccttcttgaa tgaaaaacaa tcaaatgctg
     5761 acaaagccct acatcttttt tatttataag gagtgtaaat atagacattt tgctactgac
     5821 ccgtctaagt tgttgctata tacaaaagtg tttttcctat tttattacct ctatttaata
     5881 gcatgtttat atcggttttt tgattcggat cttgtttcgt tttttaacgt atatttataa
     5941 aaataatata gtatagtagc gttctcaata tggagttcct ttggttcttt gttttttgaa
     6001 tgcttctata taccattcaa attcttcaat aaatcgtact actcttttct catagagttg
     6061 atctcttaat ctaccatcat catcaaatac atcttgaact ttggatattg aaaacgagga
     6121 agaaatggat ggcgctccta attctgcaaa tattaatcta agatgttgta cagcattaac
     6181 tcccccgaat ccaccaacag aatatgacac gattgcagaa ggtttaaaat aatattcctc
     6241 aagaaaataa tcaagtgtgt ttttcattgc tgcagatgta ctatgattat attctggagt
     6301 tataggcata taaccatctg cttcgacaat ttgatttctc aattgtttta aattttctga
     6361 tgggtttata atttccttat acattttatc taaaagtggt aaattaagtt caagggggtc
     6421 tataaaatag acaatatgct ctcgattttt caatttttct tccatccaac gagcgacttt
     6481 aataccttga cgattctgtc ttactgatcc aactataata gctatcttag ccaacgtact
     6541 attactatcc ttttttctat acaattttaa ctttttttaa caaaattaat tatttcaaga
     6601 gaaaaagtta ctttatcctt tcaaagagat atagatagag aggtcgtaag agcaatccag
     6661 gataaagctt atctttataa ataaattaga aatcaaaaga caatattcta aaatgttgaa
     6721 agttgttttt gatcccactc acgcagtctt ctcgttacct atgataagaa ataaacaaca
     6781 tttgtacaga gggtggcttg aaatttaatt tgcaacatat gatttttgag agtttttata
     6841 aaactttgct aaaaatatta attgagtaat aaatttcatg atagttatga tttattaatg
     6901 tcggttattt tatcttctac ttcccatttt tactgataga ttataacaat gacattatca
     6961 atctggctca aatttagtta cagaaaagaa gtgaacccgt ttagttatac aaactcttca
     7021 ggagatattt tccttttatt tcatatatac cttctctaga gtgaaacata aatgaaacca
     7081 taggtgacaa aaacacaaaa tatgatttac catctactga cgctccaatg tatgacgatc
     7141 ccacttggca cacattgctt ctgcttcata actactctgc aagaaagaca acaatacctt
     7201 atcaggtgtg tcggaggtcc gaacaacatc atatggcaga aggaactctc ccattcctgt
     7261 atgatagaac gcctctggtg gctgaatagg atagtccttg aatccttgag gttcaggata
     7321 ggcataagca tagaaaactg gttcatcgat aggacctccg cctggccaga aaccacaact
     7381 acttacttcg tgggaatatg cttctaccgc cacaaaacgg gccatatttg gtgcaccggg
     7441 gtgcaaaggc gctgttcgtc cagaaaagcg ggttactgcc agatcaaaag ccccccagaa
     7501 aaaatgtact gggctaactt tgccaataaa tcgggaacta aactcagata atattcgact
     7561 tccttgcgcc agaatgcgcc atacccgctg tgcatattca ggatcatagt ctacatgttt
     7621 gaaatcctcc tcaaatggag tgcgatccgg tacttcgacc ggtgttgtcc agatggttac
     7681 aggcatcccc agtaattgca aggcggccat agtttcttta taaaactcag ctactgatct
     7741 aggtctgaga ggaattctct tgatagaacc ttcatttgtt tcgataacaa gcatgtgatc
     7801 aataaaatca aagtctatct ggagtagtct atcattatag ggcatagagg acgttgtgag
     7861 tccgcgaggt gtaacataca gagtagtatt ccaccagtga ttcctgggtg gagcgagtgc
     7921 gagtcggatc ttgccgacta tttgtgtcca catatgcaat gtggcatagg tgtttttcca
     7981 ctcttccaac ggcaatgctg gccatgagtt tatagtcatt aattctcctc ctttaggcac
     8041 caatcctgat gatcacatat aatagggtaa tgatctttgg tactgcaagt tcgactaata
     8101 atatttatga gcatgatctt agcttgtatg atggctatgg ctatcagggg tttaagtatt
     8161 ctatgtaggg aattattatt attgttcatt tgtatcatat tcataagact attttattat
     8221 tattcttaat tattaaaaca tctagttaaa cggacataat taaattcaat attttatatc
     8281 aatgtctact atatgatttt taaagtagtt tcttataaaa tagacctaag actttccaat
     8341 aaactctgta tatctgggag gtaaatagta cattttctct ggtttaattt tacatattac
     8401 ccgttggatt gaagatgaat gacttggata tctatctata ttaagatatt tttttgcaag
     8461 tttatctata tgctcattag ctcctaatgt tgtctgttca atgacagttc cctgaattgt
     8521 taccatacta tatggattat ttttatcaac aagggataga gatactctag gatctcttga
     8581 aatatttttt tgttttattc ttccctgggc tgtattaatt aaaataatat tattatcatc
     8641 atcaatatcg atccatatag gagatacctg aggtctacca tctctcatta ttgttcccaa
     8701 aaaagcaaaa tttttatctt ttattatttt tatgatcggt tcgctgagta ccattttttt
     8761 ttctttgtcc tcatcaataa cactcatatt tttaaattat tatcaaatgt tgttccatat
     8821 aggtattttt aacggctcac acttgatagt gataataaaa ttattacata tactatatac
     8881 taattagatt gagctttatt gcacataata attcagaata catcaaatac aataagttag
     8941 acttaaagaa gcttcaaaaa cagattacca aaaatattta aagaattttt ttggtaatat
     9001 caaaggaacg gtcaaaagat tgaataatat gcaattctgg agaagataga taatggtaga
     9061 aaaccatcat tataaccttt tggagagcat ggatatatgg atgttattat tctaaaggta
     9121 acaatatttt atcggctcta gtagaaaatc aaactatttt agagtaaatg ccagcatgat
     9181 cagattagaa aatagtaatc tttgaaacaa attaaattgc attttaattt tatttaagct
     9241 ggaaaattat acgatatcca tggttttgca actagtatat cctcttgaat tagattgtga
     9301 cttgaaggtt gccatttcaa tgttatgatt gcattagttt tctgaaacaa tctaaataga
     9361 ttttctgtct catttctcga aactattgga tcttctaatc ctgcagataa tagtattttt
     9421 ttgcttgaaa gatttggtaa aggatcagga ataaaaggaa ccatagccct gaaaagaatt
     9481 gcgccttgaa gtacttcagg acgaagaaaa agcatactaa cagacatatt tgctccattt
     9541 gaaaatccaa ttgcaattgt attttctaaa tcaaatttat aatgcaagga acatttttgg
     9601 ataaagtctg caagttcgtg ggatctaatt ctaagatctt ctaaatcaaa aactccctca
     9661 gatagccttc taaaaaatcg tggcatacca ttttccaata cttttcctct tggacttaat
     9721 atcgatgcat taggttcaat tttttttcca aggaaaataa gatcttcttc attgccaccg
     9781 gtaccatgca ataatacaag tgtcaatttc ttatttctat catcatattt tttgttatta
     9841 gaaccattag aaaaagaatt aataaatata tgattaaatc caaaatcgtt tgatttaatc
     9901 attgctgctg gtcacttatt attgaattat ctttattatt tttggatagt tctatctttg
     9961 gtaatacttt ttctaaactt tcccttacag gttcaagcca cgttggtaac acaagcctac
    10021 tgcctaaatc ttctattttt tggtctatag taaatccggg aggatctgta gcaatttcaa
    10081 ataaaatacc acctagttct ctgaaatata ctgaatgaaa ataagttcta tcaattacag
    10141 gtgtaggatt taaacctttt tttataatat ttttcctaat cattttttga ttttcatctg
    10201 aggaagtact aaatgctata tgatgaacag ttcctattcc aatcattcca ggataactat
    10261 atggcaaaca aagaacatct aacactttag ctcctgtgga ttttgaatct acttgttgaa
    10321 agcgaaaacg gttaccttca ttttctatca ttttgtatcc taattcattt aaaatttctg
    10381 aagtaagctc atatccttct tcagataagc ttactgaata aaatcctctt ataccgttct
    10441 cataagagat tggcccatct ttccaaaatc tttcgtcgtt ctccttggct gatgaatgag
    10501 caacaagttc caattcaaga ccatcaggat catatagcgt taatacttgt tcatcatcaa
    10561 agcgtttttt aggacctcca aaacaaatat cgttcttttt gagacgatcc ttccagtaat
    10621 caatagattt ttcaggtatt agaaaagatg ttgttatagc ttgaccagta cctctatgac
    10681 cttttggagc atcagaccat ggaaaaaatg ttaaaattgt tccgggatgg cctacttcgt
    10741 ctccataata tagatgatat gtagtaggat catcaaagtt tacagttact ttaacaagcc
    10801 taagtcctaa taatgtagtg taaaaatcaa tatttttttg tggattacta caaatagcag
    10861 taacatgatg taacccagat atctggtttt tgttattcat ctgtgtatgt atgttctatc
    10921 ttactcctcc cttttctttt tttctcatta aaaattttta tctataaagt ggaaactact
    10981 gggaattctt atcaatatca aatattttaa ctattttcta cttatctatc actacactaa
    11041 atctttcatg ctaacgatta ttttcgtgcc aggttttgat ataaaagttc caatatattt
    11101 taattttctc cgccgtcgtc gtcatatgag cccactgaat agatccatcg ctcttgaata
    11161 ttaggttgaa acgtggacag attagaagag tcttcgccct tttttaattc gtcaagcgta
    11221 atttttgtca tttttacctt tcaaaaaaaa ttgaaaatca ttagtaatat ctaataattt
    11281 tagatcatct aataaagatt ttatcccaaa aatcaacagt cttttatcct accttcttat
    11341 atcattaatc tctcacaaca tattcttgag caatcaactc tcatggatgt tagaggtttc
    11401 ctccctttaa cattctatta taattaattt agtttctatt ataatatata cccactatcc
    11461 tttttacagc atatttaaat ccccgtattg tgtattaaat attatatgaa agttgaaaaa
    11521 accactagtt cctgttttga aatgcatttt gattcaaaaa cagaagaatt aaaaacaaac
    11581 cgtacacaca atcatcctaa aaattactat actttaagat atttctacaa ttcaaataag
    11641 accttttttc acctctttat gcatacagtt gtttatgata aaataacaaa tgagaatata
    11701 gagaaacaag gaatattgtt aagaagttgg aatcctataa ccaaacgagg agaacgctat
    11761 gtctttaaaa aaggagaaga aggagatggt gatttgaatt taaagaaagt ttatttaccg
    11821 aatacaaaaa caatttttaa attttctaaa agtgaagata taaaggaaga aagttacaag
    11881 ttagcagaag agataatatc agtgtgtcca cataaagata tagaattaaa atctagcaat
    11941 gattaattaa aatcattata aatagatagg tcgtaatccg cagtacataa gaaaagtcta
    12001 atatttttta tattttataa tttaatacaa taagttcaat ttaccataat atctaactta
    12061 ttccaatagt tgatgaaagt cattctatct ctgcattttc tgaatgctta acattatatg
    12121 atttaaaaca tttaattcta atattgtata ttttaaaatc ctttcatttc tgaaaaattg
    12181 tgtttaaata gatttaaaaa gtttttgaat atcaaaaata aattttgtag acagtaaatt
    12241 caggctttaa tatactattt ttttgcatga atgtttaacc taacataatc caatttccat
    12301 tgtaattctt taaagctagt tttaatttca tgagctattg ctttagcgaa cctaattttt
    12361 aaactgtcat tgtataaata ttctaaatat ggtcgtagaa caatcgtttt tatgaaaaga
    12421 aagtaatctt ctttatcatg aaatttaaca actttttttc taggaatact tcaatattac
    12481 taaagcctat ttcttttaag attgtttctg tatcctctgc tttcgcaaaa taccatggtt
    12541 ccttcaacat ttcctctcct ttactgttgt aaaaataatt acaaaattct tgtgactttc
    12601 ggacttgatt gaatacaggc agagtctttg taaagtttcc atatcctcca cattgaatta
    12661 atagtttgcc atttggtttg agaatttgcc aaaaatgtcc aaacaccttt ctgggatttg
    12721 agatccaatg taatacagca tttgaaaaca caacatctac tttatcttga agttctattt
    12781 cacagatgtt tgcttggatg aatttaacat tcgaaatttt tcctagtctt tcatttgcaa
    12841 gttttatcat cgatgaatca agatctattg caaaaacttt cccgtttgga attttagttg
    12901 ataatatctt ggttaatctt ccagaaccac aaccagcatc aagaattatt tcgtttcctt
    12961 tccagtttat acgttctatt acctctaatc cctatgattc tattacgtca gagatcttat
    13021 cgtaagttga tgcatcccag tcatatttca aacagaggta ttggtattag tatttatata
    13081 aactaacata aaattgtgtc aaaatcgttg actacctgcg cgcctcttct catagagtag
    13141 tttgaaatct ttctagagat cagaattgga gtaataaaag tacattcaac tactaattaa
    13201 tgaatcattt atctactttt acatatataa tttattatgg ttttaaaaga tgccgtaaat
    13261 tctgatatac taataaaatt atacagaatt tatgatagcc atagagatgc aatactttgg
    13321 tttttagaag aactagatgt ggatagttac gaagaataca tacaaaagta tggaggatct
    13381 tcaatccaaa gatcttattt tattgctgtt tgtggatttt ttgagttatc aggtgttatt
    13441 ataagccata gaatgataga tcctgacatt tactttgata tgttcaatcc cactccattt
    13501 tggaacaagg cagcatctgc agtagaagga atgagaaaaa aaagaccaca tatttatgag
    13561 aattttgaaa tgttaaataa taagagatta ggttggacaa aaaagcgaga gtatgatagg
    13621 aagaaaatat ctcataagat taaatagaaa agatcatatc aatttagttt agtaaatgca
    13681 attgttttgc tgataaattt taaaatataa tacgttaaca atatttaaat atatttagga
    13741 tatataataa actagtaacc atagacaaac aaataagagc aatatatatt attttagacc
    13801 ttgatgcaat taacaaaaat tttttaacga taacagaaat atccctacta caaagtctat
    13861 aagtaatgtg aatagagaac taaattacta tactataata tacttaaatg aatttgagag
    13921 tcattgtttc atatgaaata tcttttcagg ttttatcttt aaaagtactc ttttttctcc
    13981 cggagatctc ccaggatatt tatctttgcc aatatatttt tttgccaact tatctatatg
    14041 ttcttcagct gcatccccac taatttgttc tattactttt cctctgatcg taaccatatc
    14101 atatgggtta ttttggtcag atattgctaa tgcgactcta ggatcgcgtg aaatattctt
    14161 ttgtttaatt ctgcctatcg ccgtattaat aagaatatta ccattttcaa tatctgtcca
    14221 agtaggtgtt acgtgaggag agccatcttt cattaatgaa gacaaaaaaa caaaattctt
    14281 atcttcgaaa agttttcgaa tatgcggatc attaagatta tccttgtttt gtttactcac
    14341 aattaaatac ccacattatc tctaataact gttctataaa attgtgtcaa aaaaacagtg
    14401 gagtccagtt ggttgaacac ctacgataag gttatagtta tctaatgaag tggagagaga
    14461 tactcagtca tttttaaatt ttagcaaata ctaacaaaaa acacggtaaa ttactatttc
    14521 ttgcttaata ttgtattatt taaattaaac aaaatcgttt tcagtattga aattactaat
    14581 aaagattagc agagacttta gagtcaataa agggtaatta ttataatcat aaatatttag
    14641 agaaaaatta ggattattgt caaaatgatt tcattataaa accctagcct tctaaaaaaa
    14701 caactgaaaa ttcttcaatt ctttaagaag ttttggtata attattttgt taattaagcc
    14761 ttgatttata tttatttcta acataatttg ttttaatgat ttcatattat ttgaatttta
    14821 tattttagtt tatattttat tattatcaat ttaaaaaggc tttataacat agtgtttata
    14881 tgttgtctaa ttttacttta catttaataa tttctgatct taataaagaa ccctacttta
    14941 ctgtacctat gttgcagttt tttttagagt ttctgcaagc cttcgtaatg caggggattt
    15001 atcattagaa tctaggtgaa tatccaaaat actgaaactt tcagtattac ttttggcaca
    15061 aagcaaagca tctataaatt gttcttctgt ttctacaatg aatcctttac ctccaccaac
    15121 tacctgtgga attagactat attgccaggt taggatatca ttaaaactac catctaaaat
    15181 agaacgttct gtaccatatc caccattatt taatacaatc acaataggat ttaaattgaa
    15241 tcttaatgct gttgatattt ccatacctgt catttgaaaa gcaccatctc caactataac
    15301 tataggtcgt aattttgggt tggctagttg agttccaata cttgcaggta ctgcaaaacc
    15361 catagaagca taatatgcag gagacaaaaa ttctgtttta cgatggatta ctaaatccgt
    15421 acctccaaat aaagcatcac caatatctgc caagacaatc atatcatctt ttaaaaatga
    15481 attaatgcac tgaaacatac gattaattgt aattttttga ccttttatag cataaaattt
    15541 ctgaagatta tgtgactgtg gaatattaat aatattgaca cgttgtttaa tatcctgaga
    15601 ttcaattaag ttgataataa aatcctctaa acttatatct tcataattat ggtatctgac
    15661 agaaaatttt tcactattta tacaaatagt tcttctctga tcaatctttg cagtaaatcc
    15721 tccaagatca atatctgtca ttaaagcacc caaaagaatc aaacaatcac tagattctac
    15781 atattcccta acagaatcat gtcctatagc accttcataa actcctaaat ataatggatg
    15841 aagttcgctt ataacagatt tacttaaaat agttgatgat acaggtattt tgagtttttc
    15901 aataagatgc agcaattcat tttgtaatcc aaacctctgg atttctactc ctgctaagat
    15961 aacaggtcgc ttagaagaat taatcatatc aatagtctcg ttaagcgctt ccctcagtat
    16021 gataggatca ctcttttcct ggtattgatc aaactcagca ccattattcg aactatcatc
    16081 cacaaaaata tctactgaaa cgatatcacg aggtagttcg atatagactg gtcttttata
    16141 acttaaggct gaatttaaca ttcgatcaat ttcttgtgca gcagttggag gattatctat
    16201 tagtacagat gcaacagtta tttcttcaaa taccttaaac tgagtatcaa agttctttac
    16261 cttatgatga agcaagggat cctttctacg ttcctttatt cctggagacc cactaataat
    16321 tacaagaggt gatttttcag caaaagcctc tgctgtagga ttaacaatct ttaaccctcc
    16381 aacacagtac gtaacacaag caacacctaa tccattaata cgagcatagg catctgctgc
    16441 gaatcctgct ccttgctcat cacacatatt aattacttta attttactat tcattagttg
    16501 attaaagaat ccaagtacaa agtctcctgg aaccccaaaa acatgtctta tgccatgatt
    16561 aaatagtctt tctatgagat aattccctat gctaacggta ctaccaattg caagattttt
    16621 cttgtccaaa ttctatatta tagaatattg cttatcatct ataagcaatt atttagtaat
    16681 taaaagttta aataaataat atacattctc attggaatta cttaacaaat gagtattcac
    16741 ttttcttttt acttccagtg aatccacttt gagaactatt atcataatga ggagctgatg
    16801 gatgtgacca aatttgacct aaaaggtatt gctaagatta gatctctata gatgggcttg
    16861 agttaaatcc gaacaaaaaa atcagctttt gataaatcag agttatcaaa tatggttttt
    16921 ttaagataag cgcgcgatga aagttaccat cattgaaatt catcccacta atatcagtat
    16981 catccaaatt tagaaaactt aggttaattt tgttggtcgt taatctcaag atatgaataa
    17041 ctttataata tttcctagat gtatatatta atccttgatt ttaattcaat aatactgatt
    17101 caaatatact tattaaagtc gattgtaaat tcaaatacat taatttttaa ttagtttttt
    17161 gatctttatt tattatggtg aagaatacgg gtaaagaaaa agaagaagga aaaagaagta
    17221 aaaatgataa tagtgacaca aaagaggaag atctttctga aggtacaaag aaaagaatag
    17281 atgaacttcc ttcccatgct caagaaatat ttaaagaggc tcatagtagt gcattggaac
    17341 aatataaaga tccagataaa agaagaggag gaaagaaaga gagtgcagaa gaagttgcac
    17401 ataaagtagc ttggtctgct gtaaagaaaa aatataaaaa agataaagat gatgaatggg
    17461 taaagaaaaa ataaaaattt accttttttg ttaattcttt tatatcgagg ttacacgcaa
    17521 tgaactaatt ctttaaatta aatattttta ttctaaatct tttaaaatta tctatatatc
    17581 aacatgataa tattatcaaa tataagctgc tttacttatt acttcagata gtgatggatg
    17641 tatatgtata gttcttgtaa tattatcgat ttttccttca tctttgtcca ttctcattgc
    17701 aaccaatact tcatgaatta aaattgcggc atcagttccc ataatatgac atccaagaat
    17761 tgttctattt cttttatcaa ttagaaattt tacaaatcct tctttatcct ctattgcttg
    17821 ccccattcct gtttgaatat atggataaat agatttttca tattctatct tttcttttgt
    17881 taattgttgt tctgtatatc caacacctgc aatttgagga tttgtaaaaa tagcatgagg
    17941 cattgcagtg taatttacaa gtattttttt gtcaggatga acaatattat tatatgcata
    18001 ttgtgcttca ttattcgcat tatgtttaaa ttgatatttt ccaactgcat ctcctaatgc
    18061 aaaaattcct tttatatttg tctctagata ttcatccaca actatgaaac ctttttcatt
    18121 tattttgact ccactttttt ctatatttaa catatcagaa tttggtattc ttcctgttgc
    18181 aattagtaat tgatcagaac ttaatttcac agaatctcca aatttgttct ttgctattac
    18241 attaattatt tctgttttat tcttaccatt tgtttcttta gaatcataag atacttcttc
    18301 agcattatat cctaaataga tattatatct ctttgagaat atatctgaaa ttctagttga
    18361 aatatctgtg tcttcctgtg gaattaaaag atcattgcgt tgtattatat tgattttagt
    18421 acctaatgaa ccaaagaaat gagccaattc acaagcaata tatcctcctc ctattatggt
    18481 aagagttttt ggttgttgtt ttaagcttaa tgcttcattg cttgttatat attctacatt
    18541 ttttaagccc tctatattag gaatatttgg tcttgtccct gatgcaatga gaattgtgtc
    18601 tgaagttata acttcatcat ttgctatcaa gatattcttt tctcctatga atctacattc
    18661 ttgtaaaaaa agtcgaggat ttttacttat ttgtaatcct tttttaattt catttgcatc
    18721 tctatcaaca attttattaa ctctgtttat tattttttga aaatcaattg aataatccct
    18781 cactgtaatg ccaaaagtgt ctgctttttt tattatttca attatatctg cactatgtat
    18841 aagtaatttt gaaggtatac atcctgtgtt aagacaagta cctcctagtt tatttttttc
    18901 aacaatggca acttttaacc ctttctctgt tgctgctgca ttagccacat ctaacccaga
    18961 accagagcca ataacgatta aatcaaattt tttcatatat gagatgatat attttattat
    19021 ttttaataga tgttgtcata aagtggagtc aattcttaat tttttcctgt gctattttag
    19081 attaatcaaa gtattttatt ttagtactaa aagataatca atatttgtaa ggataattct
    19141 tatagttata tgttctacta ttttcctatt attatcggta atcctgtttt aactacaact
    19201 tttgttacat ctctctttta tcaaacatat tatttatctt atatcttcac ttaaccattt
    19261 cttttctttt attctcactt gaatcttttt gaattaaagt aaacatcata tgataaaaag
    19321 agattcctaa ctttcttgcg tgataatcaa tacttcttat agcatatgat aagtgttcat
    19381 catcagttga agataaaatg aattcatcta tgattttttg gaatctgtaa tcgttataat
    19441 gatagaacat tgatctggtg tgagcaatat actttttaaa ggctttataa aaattttagc
    19501 gagttaaggg tatgatagta atttaattca atttttcatt cattttattc atattagatt
    19561 gggagttcca ttacaaaagt aaatcaagaa ttatagtcga aaaatattat gagaattttt
    19621 gaataataaa agatcacgtg attgtttatt aagtttaagt ttatccacaa actttatgta
    19681 actatccata ttggaaatag gccaatcact tccatacaac aaacgatggg gcatatcaag
    19741 ataacataat agttccctta ttttatacat gtaatatctt tgatcatata atttaaaatt
    19801 tccgattacc aagcctgaaa tatctgcata gacattttta tttttgtata gtatttcttg
    19861 acaatctaat atccaaggat ttcctaaatg acacataata atagttaatt caggattatc
    19921 aacagctata tcgtctatat tcaatggatg agcaaagcga accttagaaa tattggaaaa
    19981 tgtatctcct gtatgaatca taacaggaac tttatgttct atacaaatgt cataaattct
    20041 ttgatatctt ttatcataag gataataata ttcatatcca caatatagtt ttataccttt
    20101 gattaatcca tcttttagcc attctctgca ttttctaaaa tattcatcat tgtgattatc
    20161 aattgtaaat ccagctacta tcccaagctt atagtcttta ttttttaaaa tatcaataat
    20221 ttgagatgtt gatggtctct ccatatcaaa tttatatgat gttaaaataa tagaataatc
    20281 tatattgtta ttattcatag tttttagcag ttctgtgagt ctgtctttta atgatgaaat
    20341 tgttttactc ttgtgaatat tactataata atttaggtga acatgacaat ctataattga
    20401 catatatgct ttaatttatt tgaatttaga tttatacttt gaattttaat agttttatta
    20461 atttattgtt attatgatag atattagaaa ctggtataat aaattaaaac ccgttgccta
    20521 gaagatttgg tatacataac ataccgttta ataaattatt atcattaata gagcctagta
    20581 tattgtaatt catttgaaga aaataaatcc aattttacct aagacaaaaa tattggctac
    20641 atattccaga tttagatatt atatttctat ttttgttttc ttattgacaa tatttacaat
    20701 caaacttaaa tttggttatt aatcttattt atacttgtta ttatgactga actattacac
    20761 cctcatcaca ccggggttaa agttagaaat actataaaaa gtgatattcc aaagattata
    20821 gatctacaaa agcaatcatt tccatttctt gccaaatatg gtaatatatg gaagacagaa
    20881 gagctcgaaa gtcatcttga tatttttcct gagggccaat ttgttactgt cgaaccagat
    20941 ggcacaatag taggttcagc aagtactctt atagtgtctt ttgatccaga atataaagaa
    21001 cacacatgga tggatattac tgcaaatggt atgtttacta atcattctta tgatggtgat
    21061 agtctgtatg gggcagatat ttcatcacat ccaaagtata ggcatgaagg tataggaagc
    21121 atgctttatg acactagaaa agatttgact atgaaaatga atttaaggag aatgattgca
    21181 ggaggaagat tgtttaacta ttgcgaatat gcaggtaaaa tgtcagcctt agaatatgca
    21241 gagaaggtaa ttagacgaga acttcgagat cctgtattat catttgaact tgataatgga
    21301 tttaaattta taaaaatact tcccgattat ttcgatgatg tacgttcttt gaactatgct
    21361 agttttatag aatggttaaa tccaaagatt tagttctggc ggcgagaaat cacgctgtgc
    21421 agaagtagca gcaaatatag cagtagatgt cttgaagtta gaaagtgaag gtatcaaagc
    21481 aattaaagaa atttttacaa acggattgta tgctaacaac aatatcaaaa tacaaagagt
    21541 attggaaaat cttcgatcaa aagatgatga taacatagaa tctaataata atagtaataa
    21601 taacagtaac attagcagta gtagcagcga caataatgaa atgtcaacag gattaaatga
    21661 ggatgaagaa gaattaactg atgaagatat tgacaacaac aagaaatttt aattaattct
    21721 gaatgaataa atcaaatatt agtacaataa caagccgaca attagatttg tataaattat
    21781 taagtaatag acaaaaacat aattggtgag ttatacctct acagaatgac aagaataata
    21841 gaagaaaaca gagattatat ttatatagac aggtctattg attctttatg tttatttgta
    21901 ctggaacgtg taaactagtc aaattagaaa tttaaagcgt tctaaatgaa taatagcgtt
    21961 cttttaatac atcaattctc tgccttaacg tcattgctgg cagtttcaaa tcaaacagtt
    22021 cccaggtgtg tgaaggtgaa tttagatcgc cattagacaa tttttgtaga catgaaattg
    22081 cactccgaag atctcccgtt acataattta atgcaatcat atctgcttca tattcattat
    22141 tttttaaatc ttctgcatta ggcggaagat tattaggact aagtaatgtt agtaatccct
    22201 tgaatgtctc tacaattagt ggtgcgttat aatctaaatt tttcagaagc cgttctatta
    22261 tgttccacat caaggtattt aaactatgat ttttatagat atgcgctgtt tcgtgagcaa
    22321 gaataaattg aatctctttt tctgtaaatt gtccttgatc taaataatct gaattaatta
    22381 aaataattcc tttagtatta ataggccaaa tagtagaatt aataccaaat gcacctgcaa
    22441 taagggaatt tttagtattt attacaataa cattgtgatc gttaattagt aaacgattag
    22501 tcaatatcga atttacataa tgttgtgacg atttaatatc taacattcta tttattatat
    22561 gttaaagtat tatataatag gaagagttaa gacttaagat aaaaaaacag tcaaatacga
    22621 atattatatt ctattccact gatcaaacca aaattgagtc tagcaattca ttcaatgttt
    22681 tcttgcttca tctttctcca tttcatctaa aaatttgttt ttaattttgt atgattcaat
    22741 acaaatatcc ctgattattg tggtttagtt tcagataaaa taatacttca taccaatgca
    22801 caacataata aacctttttt tagtatgagt ttgaaggtaa gaataacaga tatcaaagat
    22861 atataaaata aggttattga aatagttcaa aaataataaa agaaatagtg aaccttgtcg
    22921 ttccaattgt agaacattta gaaatattac attaaaaaga taatcttatt tactaaagaa
    22981 aattatattt taatatgaaa tatactttat ttatattatc agtttttccg ttactgttgt
    23041 tttttttgat tctcttaatg tttgtatcaa tacaacaaca agcatttaca gaaactgctg
    23101 aatctatctg gtccattgga aaatctatgc ctacacctag aacagaagtt actgctacta
    23161 tcattgatga taatatttat gtcataggtg gatttgacaa atctggcaca gtattagata
    23221 ctgtagaagt ttataatatt aagaatgatt cttggaagac aatagcacct ttaccacaac
    23281 ctgttcatca tgccgctgta accacattta atggcagtat atatgttata ggaggatata
    23341 caaataataa ttggctgcct tcagccaaat tatatattta cgataccaaa aatgatacct
    23401 ggattgaggg atcctctatg cctacggcta gaggtgcatt aacagccgtg tttatagatg
    23461 gaatacttta tgctatcggt ggtgaaggcg aaaatagaat tatggatact aacgaagcat
    23521 acaattcaaa aaccaagacc tggatagcaa aatcatcaat gcctactgca aggcatcatg
    23581 ctgcatctgc agttgttgat ggaaatgtgt acgtaattgg aggaagaata gaaggatctt
    23641 tacccattac caatgtgaat gttaatgaga agtatgatgt taaaacggat atatggacca
    23701 cgcttgaccc aatgccttca gaaagaagtg gaattagtgc aggatcaatt aataatacta
    23761 tttatgtatt tggtggtgaa gatattacta aaacatataa caataatgaa aaatataata
    23821 ttaaagataa caaatgggaa tctaaagaac ctctgcctac tgctcgtcat ggattgtcag
    23881 cagtgtctgt taatgataaa atatatgtaa taggaggagg acctgaacct ggattgaacg
    23941 tgactaacgt taatgaaatt tttaatacta gagaataaaa atctcttgta ctttctaata
    24001 atatgattat tttaaaaggt aatctttgaa aaagtaatcc tgttaattag gtaaaacatg
    24061 gattaataat aaattattat tcctacactt ttatcattca acctgctatt tccgttctag
    24121 gtgtagacat agatttttct atagtccaaa atgattctcc ttcaattctg cataaatagt
    24181 ttgaaaatca aatgatgtag tattatcatc tcgtttcctc atttggaaaa acactagata
    24241 ggattggaat ggggtctaag gacgaatcaa atgaaaatag aagaaagata actttacatt
    24301 cttttagacg ctttgttaaa aaccaccatt tcagatttgg gatatgcaga ttattctgaa
    24361 tggtttattg gccatgctgg atctacatat tggagaaaaa aagataatga aaaagcagaa
    24421 atattcaaaa agatagaaca gtggtaagga ttatatttga ttatcgaaat tttccaagtt
    24481 atagaatctt tatattgcat tttcttacga gttctgcttt cctgataatc cacccattat
    24541 caaataacga tagtgggcaa tctactgact aaatgtagat tcatatttaa tgttaatact
    24601 tatgaagaaa atccaactag taacaataat acaattatta aattcaagtt aaacgacgta
    24661 tctaatgaag tcatcaataa tagatttaag gatgaaccat atccaactgg ttattacaaa
    24721 aaaataaaaa caaggattga taatttcagt ttttactaat tgttcttaaa atatgaaata
    24781 ttattggtat ctaaaaacaa atctttttta taggttttct tttgatataa tatattgaaa
    24841 ctaatatcaa atatacagac tatatattac attttgatgt tagtttttat atcattattt
    24901 aactaccaac aggtgtacgc tgtactagaa tatataaatg aaaaacacgc tgtcaatttg
    24961 aaatattctg acgattggaa attcgataaa gacaaacaaa ctgagatagg tccagaactt
    25021 gctaacttat taaataaaga agtggataaa ttcgtaaatc cagattttaa tcatttccac
    25081 ttatttcaat tagttccgta tattgaaaac gatgggtatg aatcaatatc agatggtaat
    25141 actgtttttt ccgtatccct atatactaat atgtctagtc ttgaagacta caaggtatat
    25201 cagaatatga ctggtcttgc agatactaga gtatatacta atctgtccag tttaggtgac
    25261 aaagtatttc ctaatatgtc tagtcttgaa gactacaagg tatatcagaa tatgactggt
    25321 cttgcagata ctagagtata tactaatctg tccagtttag gtgacaaagt atttcctaat
    25381 at
//
LOCUS       DASVEV010000140         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_828590, whole genome shotgun sequence.
ACCESSION   DASVEV010000140 DASVEV010000000
VERSION     DASVEV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828590"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..697
                     /locus_tag="VF222_06365"
     CDS             290..697
                     /locus_tag="VF222_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142083.1"
                     /translation="MKNNPFFMIFVIIITTITGTSLFIWTQNVDASSNIPCCGTMTLY
                     NKTPMSVQISLQIGDPKYDATCTADPNSSCTAKTANADYDTIKVGIPNLSNSAWEKEF
                     NGINGQCDYYVSGSDGNYKAQINCPIPDDKKHK"
     gene            1261..1410
                     /locus_tag="VF222_06370"
     CDS             1261..1410
                     /locus_tag="VF222_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142084.1"
                     /translation="MSVMDGFDLYDKLLKISIKVEDTPTKEFKICFMTSSVINYKVLA
                     GIHRN"
     gene            complement(1524..2129)
                     /locus_tag="VF222_06375"
     CDS             complement(1524..2129)
                     /locus_tag="VF222_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142085.1"
                     /translation="MTNYTIQPFSNSKKLSIFVFFATSLLLTSTYSYAAECMSDVMAN
                     KTKQNIQEKSTESIVKSKQQQEQRINNNILSPQSSQQLQKENEQKTENIRVITTLLNA
                     ADQLTGLAKVTVTFENENNEQITFQSNNKTITAEEEIVFPLTLTKPITEKEQQPSTMD
                     VCAVTEGVENECDTVLLVENQTKPYKVNLELINFEEVEPIN"
     gene            complement(2251..2595)
                     /locus_tag="VF222_06380"
     CDS             complement(2251..2595)
                     /locus_tag="VF222_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142086.1"
                     /translation="MTLNIQKTRQLITHSDEEKELVKKIESITKSLRKQYFKNSLVTL
                     SKKNIENARTICNYIIAKQTEINIKNSTKESRIKVLVWLSNFLEDKISFKEMTKQDIL
                     NYLNILCSDYLK"
     gene            complement(2702..2785)
                     /locus_tag="VF222_06385"
     tRNA            complement(2702..2785)
                     /locus_tag="VF222_06385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2749..2751),aa:Ser,seq:gct)
BASE COUNT          925 a          400 c          415 g         1102 t
ORIGIN      
        1 aattcctgat ttgcttttcc atgttctggt tttgatttta tagataattt tatttcgttt
       61 ccatttattt gaaattcaaa gaatgttgcc gtcccgggat ctgtgaagag tgagaaagtt
      121 ttaactaaga gtagaatcga ctgttagaat atagttctcg actctcattt aattctctat
      181 taccgtctac gagaacttca aattatgcta tccctgaaat gaaataatat tatttgcaac
      241 aatatggggt tgagataacc ttataaatac aataaatcaa ggcatcataa tgaaaaataa
      301 tccttttttc atgatatttg taatcatcat aacaaccata acgggaacat ctttgtttat
      361 atggactcaa aatgtagatg cttcctctaa tattccctgt tgtggaacta tgactttata
      421 taataagacg ccaatgtctg ttcaaatatc tttacagata ggtgatccta aatatgatgc
      481 tacttgtact gctgatccca actcttcctg tacagcaaaa acagcaaacg cggattatga
      541 tactattaaa gtaggaatac ctaatcttag caattctgca tgggaaaaag aatttaatgg
      601 tataaatggt caatgtgatt attatgtctc cggttctgac ggaaactata aggcacagat
      661 taattgtcca atcccagatg ataaaaaaca taaatgacaa taataatgca agagcattag
      721 aagagatgtt actaaaaata ccctatcatt ttgattttca tattctacta tttatagata
      781 aaaatcttaa ctaataaaca ggagactatt accattattc ataagatatg gttctatatt
      841 ctagtattaa aatagttgat tagttaaaaa aaatagatgt ttgttatgat atataatttc
      901 aagttattaa caatattatc attctaaata actaattctc agattgggac tcgaaccctg
      961 caactacatt cgcgggttta tgctattaaa atatacaaaa agattaaatg aaatttttag
     1021 attgtgttaa agcattttct taaaattatt gttacagaat tggcatggag gggatgggat
     1081 ttgaagcctc ggttcgatgt cttgcttttt gagccttcat aaattatctt ttaccaatat
     1141 gaaataataa ctttatgcta attaataata ttcaaaatat tagtttcaat aaatgatcca
     1201 caattagccg aagttaaatt taaacctcag aactatgctc ttgtacttgt tggttttaga
     1261 atgtcagtaa tggatggatt tgatctttac gataaattac ttaaaatctc aataaaagta
     1321 gaagatacac caacaaaaga atttaagatt tgttttatga cttcatcagt aataaattac
     1381 aaagtgttag caggaataca tcggaattag gagaagagtg ttatgtttca aaagaggttc
     1441 tcaaagaagt ttttataaag tatgtctatt cgttaacttc ttaatattat gataaaaaat
     1501 gaaattattt attgaaatga attctaatta ataggttcta cttcttcaaa gttaatcaat
     1561 tctaaattaa ctttatatgg ttttgtctga ttttctacta gtaagacagt atcgcattca
     1621 ttttccactc cttcagttac tgcacagaca tccatagttg atggctgttg ttctttttct
     1681 gttataggtt tcgttaatgt taaaggaaat actatttctt cttctgcagt tattgtctta
     1741 ttattgcttt gaaatgtaat ttgttcatta ttttcattct caaaagttac tgtaactttt
     1801 gccaatcctg ttaactggtc agcagcattt aatagagtag taattactcg aatattttct
     1861 gttttttgct cattttcttt ttgcaattgt tgtgaggatt gtggtgatag aatattatta
     1921 tttattctct gttcttgttg ttgtttagat tttacgatgc tttcagtaga cttttcttga
     1981 atgttttgtt ttgttttatt tgccataaca tcagacatac attcagcagc ataactgtat
     2041 gtagatgtca ataacaaaga tgttgcaaag aatacaaaaa tagaaagttt tttcgaattg
     2101 gagaagggtt gtattgtata atttgtcatt aaataaatct acattttatt ttatatatgt
     2161 attatagtgt ttattgactg tttatttgat aaattgatcc ttttttactc tatttctttt
     2221 ttttatatta tctaatatta attgctaaca ttatttgaga taatcagaac ataatatgtt
     2281 taaataattt aagatatctt gttttgtcat ttctttaaag gatattttat cttcaaggaa
     2341 attggataac caaacaagaa cttttatcct gctttcctta gttgaattct ttatatttat
     2401 ctcagtttgt tttgcaataa tataattaca gatagttcta gcattttcta tatttttctt
     2461 agataaagta actaaactat ttttaaaata ttgttttcga agtgatttcg taattgactc
     2521 tatctttttt actaattctt tttcctcatc actatgtgta atgagttgtc ttgttttctg
     2581 tatattaaga gtcaaaatta tttatcatct ccttcttttc ttctctttcc ttgttgttta
     2641 tctaacgata agtatctcgt atcaaatctt ataacatttt tggaggaacc cccaagataa
     2701 gcgcggggag tgggattcga acccacgagt tctttcgaac atgggattag caatcccacg
     2761 ccctaccagg ctaggcgacc cccgctcaaa gatagtactt gtatgaaata tactactgtt
     2821 taaaaaataa agacatatta at
//
LOCUS       DASVEV010000141         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1217595, whole genome shotgun sequence.
ACCESSION   DASVEV010000141 DASVEV010000000
VERSION     DASVEV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217595"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..214)
                     /locus_tag="VF222_06390"
     CDS             complement(92..214)
                     /locus_tag="VF222_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7142087.1"
                     /translation="MKGKKVYDMALDGSNGSFYMNIHTLENPNGEIRGTFTIPP"
     gene            complement(361..483)
                     /locus_tag="VF222_06395"
     CDS             complement(361..483)
                     /locus_tag="VF222_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142088.1"
                     /translation="MFVIVITTKLPGSEEVPPVQTNATGMAEISAYTVAEDTIT"
     gene            473..619
                     /locus_tag="VF222_06400"
     CDS             473..619
                     /locus_tag="VF222_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142089.1"
                     /translation="MTNNNDKDIKIVTGEDVGKDDKIKAGVNSLAKKPFRETSAEYKA
                     DSLE"
     gene            complement(982..1191)
                     /locus_tag="VF222_06405"
     CDS             complement(982..1191)
                     /locus_tag="VF222_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142090.1"
                     /translation="MAISLPVILLLGLVESSTEDAGSGRIIFIVMFILGIAYLVFWIW
                     QIFNARKLARKFNELVRTNGGKEPW"
     gene            1211..1339
                     /locus_tag="VF222_06410"
     CDS             1211..1339
                     /locus_tag="VF222_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142091.1"
                     /translation="MMIPETNIPNPFPIFPTNIWPSPSRANIPPINAMSAAVPGLL"
     gene            1911..2837
                     /locus_tag="VF222_06415"
     CDS             1911..2837
                     /locus_tag="VF222_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX7142092.1"
                     /translation="MDIKPQNILDYHGSNAIFMVWNFKKDLDTKDTFERICKLVINLN
                     NSADTRFPDSGASCVMGIGYNAWLQLHLPIPIPKELENFVPIVGEKHTAVSSKGDLHF
                     HIRGNGSSVCYDMAYAVSDVLEPVAISIEEIHGFRYWDGRSILGFVDGTENPHGQERA
                     FFGLIGDDDPVYKGGSYLFVQKYTHDLNAFEELSTEEQEKVFGRYKLNDIEMPDNIKP
                     SNSHIALANVGDEFKIIRDNMPFGNISTNEMGTYFIAYASTFSTVKKMLNNMFIGSPK
                     GNYDKLLDFSTPKTGTLFFVPTLDMLDDFSSG"
     gene            3276..3482
                     /locus_tag="VF222_06420"
     CDS             3276..3482
                     /locus_tag="VF222_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142093.1"
                     /translation="MRAITSKRENNQTPTFYIMVESIDDFIAKSQKQGAKVIVNKQEI
                     SEGFYATIQDQQKNTFGLWQSKNK"
     gene            3723..3923
                     /locus_tag="VF222_06425"
     CDS             3723..3923
                     /locus_tag="VF222_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142094.1"
                     /translation="MFQHHNDDKYKKIIDEFILSSSDNESIKYILRNVDFEAFKLGIS
                     FYQMIFILIQKNAVHNRKSQIR"
BASE COUNT         1715 a          736 c          703 g         1572 t
ORIGIN      
        1 tctaagacag taatgccgct ctcttgaggc ttcctgctcg caacatcaat cttgtatcta
       61 ttcctgcaga ttattaagat tcggtgctat actatggtgg aatagtaaat gtaccacgga
      121 tttctccatt aggattttct aatgtgtgaa tattcatata gaatgaaccg ttagaaccat
      181 ctaatgccat atcatatact ttcttgcctt tcattggtcc ctctagttta tcatctgtaa
      241 ttgtaccgga ttctgatacc tagttcttag gagggtcata tttgaacagt gtaaatacta
      301 taggcccatt ttcaccttcc ttagtcctgc tgtgagatcc ttaatattca tagcattcac
      361 ttatgttata gtgtcttcgg ctacagtata tgctgatatc tcggccatgc ctgttgcatt
      421 ggtttgaact ggagggactt cttcactacc tggtaactta gtggtaatta caatgacaaa
      481 caataatgac aaagatataa aaattgtaac aggtgaagat gtaggaaaag atgataagat
      541 aaaagcaggt gtaaattcct tagccaaaaa accttttaga gagactagtg cagagtataa
      601 agcagattca ctagaataaa gatatatatt ttattttatt gttatattaa gactaattaa
      661 tctattactc tagtgatata tcatagatat atatagagaa tatcattcat actgttttac
      721 tatattttat acaaagagat attttcaaaa atgataaata ataatttagt tattcttttt
      781 tgcatcagaa aaaaatcaaa aaaaattatt ttatcgattt aagccatgcc aaaaaaatta
      841 aacaaactga caattaagat gataaaaagt aacaaataat actacttcct attaacccat
      901 taaattcata atatattgta tttttttcta accaagttct tagattggga ataaagaata
      961 gaaaagtttt ggtttagatt ttcaccatgg ttcttttcca ccatttgttc gtactaattc
     1021 attaaatttc ctcgccagtt ttctagcatt aaatatttgc catatccaaa atactaaata
     1081 tgcaatacct aagataaaca taacaataaa tattattcta ccactgcctg catcttcagt
     1141 agatgattca acgaggccta acaataatat aactgggaga gagattgcca tgatgacaaa
     1201 cagaacgtag atgatgatac cagaaactaa tattccaaat ccttttccaa tctttccaac
     1261 aaatatatgg cctagtccta gtagagcaaa tattcctccg ataaatgcaa tgagtgctgc
     1321 tgttcctgga cttttgtagg gcagttggtc tacaattggt ctattatgcg taatagaacc
     1381 tacttgttgt tgttgaagat caattccaca ctttgaacat tatttaattt cttcatttaa
     1441 atttaatggc tcaccacaat tgctgcagaa ttttggtgta ctagtcaatt atatacaata
     1501 cgattaccta ctaataaaga aaaactttaa ggataataaa aaaatttagt aatttattta
     1561 gtcatttcta tgcacacatt tctatgcaca tttctgcact agaggggcaa ggagatttgc
     1621 attcagtttc tacttcttct attgcttgtt gactataaga cgtcatagta gtcttatgat
     1681 ggttaacaac aaaattctat ccttctccaa gagcattatt agattcaaaa aatataggac
     1741 atagtaaggc atacactatt gtagttatca caacatactg agacggaatt gcaataaagt
     1801 tttccatata ctttccttca atatatataa taaatacaat gtccttaagt taacaaagcc
     1861 atcaaaaatc tgcaaacttt aaatgaatac ctaatatatc tttatttaat atggatatca
     1921 agccacagaa tatattagat tatcatggta gcaacgccat atttatggtg tggaatttta
     1981 aaaaagattt agacaccaaa gatacttttg agaggatttg caaattggtt atcaatctaa
     2041 ataattcagc tgatacccgt tttcctgatt ctggggcaag ttgtgtaatg gggatcggat
     2101 ataatgcctg gcttcagctt catcttccta ttccaatccc taaagaatta gagaactttg
     2161 tacctattgt tggtgaaaaa cacacagcag tttcttccaa aggagatttg cattttcata
     2221 tcagagggaa tggtagtagt gtatgttatg atatggcata tgcagtgtct gacgttttgg
     2281 aaccggtggc aataagcata gaagaaatcc atggtttcag atattgggac ggtcgttcca
     2341 ttttaggttt tgttgatggt accgaaaatc cccatggaca agaacgggca ttctttggtc
     2401 tgatcgggga cgatgatcct gtttataaag gaggaagtta tctttttgtt caaaaatata
     2461 ctcatgattt aaatgcattt gaagaattat ctactgaaga acaggaaaaa gtatttggca
     2521 ggtataaatt gaatgatatc gaaatgccgg ataatataaa accttcaaat tctcatattg
     2581 ctttggccaa tgttggcgat gaatttaaaa ttattcgcga taacatgcct tttggaaaca
     2641 tatccaccaa tgaaatgggc acatatttta ttgcttatgc cagcacattc agtactgtaa
     2701 agaaaatgct gaataatatg tttattggct ctcctaaagg aaattatgac aaattattgg
     2761 atttcagcac acccaaaacc gggactctat tttttgttcc aacgttggat atgttagatg
     2821 acttttcatc gggttaaatt ttaatttttg ataggctaca gctttgtttg aatttcgaaa
     2881 gttaagaaat tcattcatca ttgcccggct aaaatattcc tgaagtgact gcatcaatca
     2941 ttttgataat ccaacaaggc taaaggaagg aatacccttt attttttgca taacttggat
     3001 taatgcaacc aaaagcctgt aaaaaaacca aagcattaca acattctaat atatccttga
     3061 agcaattaaa tgaggaactc aaaaggcaag atattatgta aaaagagttc ataaatatag
     3121 cagcacatga attgcgtaca ccagtccaat caattgttgg tacctagaaa tggtcaaaag
     3181 ttttcctgaa aattttaaaa aatatttaga accattagag cgaaattcac aaagactata
     3241 tcgattaaca gaagatattc tagatatagc aagaattgag agcaataact tcaaaaagag
     3301 aaaataatca aactcccaca ttttatatta tggtagaatc aatagatgac tttatagcca
     3361 aatctcaaaa acaaggagca aaggtgatag tcaacaaaca ggaaatatct gaaggttttt
     3421 atgctacaat acaagaccaa caaaaaaata catttggttt atggcaaagc aaaaataaat
     3481 aactgaatta aaaataattt aaaaataagt tctaataata aactgaattt taattctaca
     3541 ttttctgtca gatatacaaa aagcataacc acctgaactc aatacctttt tttataggtc
     3601 ttccttatta ttattatgag gatgatgcaa aatgtatatc ggttataaat aatttcaaat
     3661 gttaggcatt agtcaccaat gtttctaaaa gctcttaata actataataa taaaatcaaa
     3721 tcatgtttca acaccataat gatgataaat acaaaaaaat tatagatgaa tttatactat
     3781 cttcatcaga taatgaatca ataaaataca ttttaaggaa tgtagatttt gaggcattca
     3841 aattaggaat ttctttttat caaatgatat tcatattaat ccaaaaaaat gctgttcata
     3901 acagaaaatc tcaaatcaga taaatgacaa atactaaaga aattttgata tttatatatt
     3961 ttaacaataa gttctaacaa taaaagcaaa atactatgtg atagtaactt taatggactc
     4021 cctttttaga acctctatcg gtattactcc ctcctctaat tttctaacaa attccaaata
     4081 tttgaatatc aaaccatttt aaaataagtt tctaataata aaagctaaat agtatgtagt
     4141 agaaccccaa tacatggagg gcactactcc cgacggaccc gtagttttct cgggttcatc
     4201 aatttgacag taaggttaaa aaaaaagtaa atcagtatat tagaagttaa attttatcga
     4261 aagctatgaa atagttctaa atgaaaaaat tagcttctac accttatcta attatacgag
     4321 aagaaaaaga ttgacaagac tcgaatattt ataagatatt actcacaaca aacaattgta
     4381 ttgatagtga tatttctaat actcatttta tgacttatta ttcgacaaat atctatttat
     4441 ataattcata gtatttatag aactttatta tattagtaga aattaaggtg aaattcgaga
     4501 attaaaatat ttagaccata agttaaatgt attggtacta gaacccttat tcatcgaatg
     4561 tggggtagta agtataaaat tcaacctacc caaatattca aatgattata taaaatattc
     4621 taatatcttt tcaaggttat cattattagt atcatctctt gtaaattcct gataccttgt
     4681 taagtctctc ggggtcacta taccaattgg tttatttatt tccgtc
//
LOCUS       DASVEV010000142         6434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1023690, whole genome shotgun sequence.
ACCESSION   DASVEV010000142 DASVEV010000000
VERSION     DASVEV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023690"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..158)
                     /locus_tag="VF222_06430"
     CDS             complement(12..158)
                     /locus_tag="VF222_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142095.1"
                     /translation="MSSSNTTNKTAPSAIRCEICGLPFETLAAKNEHIKLEHIEHKPP
                     SGVG"
     gene            complement(772..1452)
                     /locus_tag="VF222_06435"
     CDS             complement(772..1452)
                     /locus_tag="VF222_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142096.1"
                     /translation="MITQDGKPFGEGILGSEYPLNLYYAYGVKNSFGIDFDPVTKKPW
                     LTENGPKFGDEINLAEPGFNSGSDKIFGIWRVNQEGKKLQSDSNEGGGIVGNNPEDLV
                     YFTGKGHYSPPEFIWDKSTPPTALLFVNSRNFGTDYENDMFVGSVDGGIFHFNLNDNR
                     TGLLLTGILEDKIAADETEFADILFAEGFSIITDLKQGPDGNLYVVSGIKQSKSEKYG
                     AVYRIVPS"
     gene            complement(1960..2502)
                     /locus_tag="VF222_06440"
     CDS             complement(1960..2502)
                     /locus_tag="VF222_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7142097.1"
                     /translation="MSLLTNYDRVFEFIYNNPGSHFRKIKKELDLSIGTIQYQVNKLE
                     KDGKIVSIRHRFYKFYFPNGVFQEHEKEILQVLNNASLRNVLLLIIERKNPSKHEITK
                     FLNISYSSVNWHLEQLMSYDMILENRDGKVVRYSMNNNVNYVPEIIKLLKSHYKSIWN
                     SWANRLAEIFLLLSVDEDRK"
     gene            complement(2944..3804)
                     /locus_tag="VF222_06445"
     CDS             complement(2944..3804)
                     /locus_tag="VF222_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142098.1"
                     /translation="MIIIVFSISLLSLSVINTIGIVDATTANNKEKDDDNTKNEKGTF
                     KLKIKLNLNNVPPDIQKLKLVSYVNGEVKEQYIDLSKDKSKIKENTLALNLSYDKSND
                     VSSIIVTDQYFVCAYALNDKIPKQKGVSFYDCDEGNVGSTDSSTAHLFFTLNKFNESV
                     NFNKISGQGKNPLANEVKINVKVPLEDATDANQISVVGMIGGEYKVETLNVEQALNKQ
                     ENNEGDILDVPFVFNRTTEVGQIQLGDKFFGCVTGEELSPQHSHCEKRTLKDFEKGNQ
                     LYSRKDNNFK"
     gene            4258..>6434
                     /locus_tag="VF222_06450"
     CDS             4258..>6434
                     /locus_tag="VF222_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142099.1"
                     /translation="MNKKNEIYIMVPFILAFLLFSIFNNNNNNTFAQSPAAAPSSIEG
                     IKVYDPNLKIELVANGFDFPTTMAFLGPNDFLILEKSGAVKRVTDGVVVKKPLIEVNA
                     SLKDERGLLGIAISQLKNPISQVSTQEDPKITHNIFLYYVECKNHSLECSNNIYRYQM
                     DIKNNALINPKLLLSIPSFPDPSHIGGIIRIGHDDNLYVTVGSFQNTGVPPTIKNKAL
                     NFEDGNNFDGRGSILRITQEGKPVNDGGGNIIGDTYPLNLYYAYGIRNSFGLDFDPLT
                     GKLWDTENGPYFGDEINLVEPGFNSGSDKIFGIWKNDGFDNKQREGKSKSKSENYVIV
                     NETHPDDLIYTGKGHYNLPKFMWDKTVAPTALLFLNSKTLGPNYENDMFIGSVKDGKL
                     FHFELNNLRNGLNLEGVLSDKVATNKDEFGSIIFAEGFNYITDLKVGPEDGYLYIVSG
                     FKSKQEKTEKVLKTGTVFRIVPIETTIKEDNEEIEKPPLERVFPKINILNGLSPKLEE
                     TPKIDTNILNQLAPKPSKTPKLDVKAWDDLMKQTQGATIDEKIVKLTGDEQQQMQSNN
                     LTEKNINQTSKITDDFKPGDQIVLQGSASSVSKEYPDDDEPQAHQVAVLLPPRDDKSI
                     YSGTITLTTTKPLEILSLNDYQLNKNILSNMSESFGTFTTIPSSANVSNSYIATTIAT
                     LEKKNDFGYDLSIPFLGNSLELHNTKDEPFIAVYTVRADILKS"
BASE COUNT         2249 a         1014 c          937 g         2234 t
ORIGIN      
        1 tattattatt attaaccaac tccacttggg ggtttatgtt caatgtgttc taattttata
       61 tgttcatttt ttgcagctaa agtctcaaaa ggaagaccac atatctcgca tcgaatcgca
      121 gaaggcgcag ttttattagt ggtattagaa gatgacatag attccatcta ttacctgata
      181 acgcactcga agttaataat agtttagaat aaaatcattt tcattgatat gcacaagcaa
      241 aaaatacaga taaaatttaa taatgattat tattgcagaa aataagatta aaaagtttaa
      301 ccatcagaaa gagaggctaa tttttttctg acttacgatt tagattatct actactctaa
      361 ctatatctac atagacttgt ttcctcctct ctctatttta tatcaagtct ttgttggtgt
      421 ggttgtcttt caataacaaa cttttattta ttgttacttt atatttcctt cacataagtt
      481 gcccataata agggatgatt gcgtctaaga tgtatagaac tcagcaatta tgttttcgct
      541 ttctcaaatg ttttctatat cagtacgaac tcatttagtt tatcaatagg tgtgactaac
      601 agagtatttt ctatccttct tatatcatct caagtgaaag gtggctatac cgtaatcact
      661 tgttaagtta ggacactttc ttctagtagg tgatacatca aaatcatatt ttactttact
      721 ttacttcaag agtgaaacta tcgattatgt attggtggtg gtggtggtgg cttatgatgg
      781 tactattcta taaacagcac catatttttc tgattttgat tgctttattc ctgataccac
      841 atagagattg ccatctggac cttgcttcag gtccgtaata atactaaaac cttctgcgaa
      901 tagtatatca gcaaactctg tctcatctgc agctatttta tcttctagaa tacctgttaa
      961 aagcagccca gttcgattgt catttaaatt aaaatgaaat attccaccat ctacagaacc
     1021 cacaaacata tcattttcat aatcagttcc aaagtttctg gaattcacga ataatagtgc
     1081 agttggaggt gtcgatttat cccatatgaa ttctggaggg ctataatgtc cttttccagt
     1141 aaaataaact aggtcttcag gattattacc tacgattcca ccaccttcgt tgctatctga
     1201 ttgtagcttt ttaccttcct gattaactct ccaaatgcca aatattttat cagaaccact
     1261 attgaagcct ggttctgcca aatttatttc atcaccaaat ttagggccat tttctgtaag
     1321 ccatggtttc ttagtgacag gatcgaaatc aattccaaaa ctatttttta ctccataggc
     1381 ataatacaag ttaagaggat attcacttcc aagtattccc tcaccaaagg gtttgccatc
     1441 ttgtgttatc accaaaactc cagccctacc atctgcaggt ttaccatcta caaaattctg
     1501 agcactattt tttatgttat agtaactaaa acataattac catcattcaa agacaaagaa
     1561 ttattagaat tactccatat aaaaaaaatg ttcaaaaaat atttgttaat aaatcgttct
     1621 aatttgtcaa gtcatgttaa tacaaaaata ttaacttttc acaagagaca catatagtgt
     1681 tataaggtgt gtagtataac ctcaatcgca ataatgaatg ataaggaaga ttgaaaaatg
     1741 taaaaaaaga tgatatatca attaattagg atagaataac caaatacact tcaagtcata
     1801 acattcacat tccgtatctt atttgacaaa catataacag ggaaggagat ggacttgatc
     1861 gaaattgatg agtaatcaat tttgctttgc taatataact ttaaaaaagg tatttcaaat
     1921 aataatatag tggattgtag atagtagaaa aattatatat tatttacgat cttcgtcaac
     1981 agatagtaaa agaaatattt ctgcaagcct gttagcccaa ctattccaga tacttttata
     2041 atgacttttt aataatttta ttatttcagg aacgtaatta acattattat tcatagaata
     2101 tcgtacaact tttccgtctc tattctctaa aatcatatca taagacatta attgttcgag
     2161 atgccagtta actgatgaat atgatatatt caaaaatttc gttatctcat gtttacttgg
     2221 attctttctc tctataatta ataaaagtac attacgtaat gatgcattgt ttaaaacttg
     2281 taaaatctct ttttcatgct cttgaaaaac accattagga aaatagaatt tgtaaaagcg
     2341 atgtcgtatg gatacaatct tgccatcttt ttctagtttg tttacctgat attgaatggt
     2401 tccaattgat agatcgagtt ccttctttat ctttcgaaaa tgagatccag gattattata
     2461 tatgaattca aatactctat catagttagt taataatgac attcaactac acccaaccaa
     2521 atattataat aaaatataca tttgattata tataaagcat tatacataca aagacataga
     2581 gagatataga taacattagg ataaaattaa tgtatgtaat accttaaaat tgattttata
     2641 taaccatttc taataacaaa ttgctcatag ctagaaaaaa cagtttactc attcacaaca
     2701 atactttgta aacataaaaa aatgaaaata ttataaattt atcaaactat actagaaaga
     2761 ttgttaaaat attattaaat tgaaagtttc atctgaaata tgcaaaatta accgttaaaa
     2821 aatatataaa ttttatttct ctctttggtg tttagttaga gattcacatc aatactaata
     2881 caaatttatt tataagaagc ccttggcaat aatcgtggat gtatcgaaaa atgaaaattt
     2941 gttttattta aaattattgt cttttcttga atacagttga ttaccttttt caaaatcttt
     3001 caatgttctc ttctcacaat gtgaatgttg tggagataat tcttcaccag taacacatcc
     3061 aaaaaatttg tcacctaatt ggatttgtcc tacttctgtt gtcctgttaa acacaaaagg
     3121 tacatccaat atatcacctt cattattctc ttgtttgttc aacgcttgtt ctacatttag
     3181 agtttcaacc ttgtattctc ctcctatcat acccacaaca cttatttggt ttgcatcagt
     3241 tgcatcttca agtggaactt ttacattaat ttttacttca tttgcaagag gattttttcc
     3301 ttgtccactt attttgttaa agttaacact ttcattgaat ttgtttaatg taaagaataa
     3361 atgagcagta cttgaatcag tagaccctac attaccttca tcacaatcat aaaatgaaac
     3421 tcctttttgt tttggtattt tatcatttaa tgcataggca catacaaaat attgatctgt
     3481 gactataata gaagatacat cattactctt atcatatgat aaattcaagg caagtgtatt
     3541 ttcctttatt ttgcttttat cttttgataa atctatgtac tgttccttta cttctccatt
     3601 aacatatgaa accagcttta atttttgtat gtctggtggg acattgttta aatttaattt
     3661 aatttttaat ttaaatgtcc ctttttcatt tttggtgttg tcgtcgtcct tttctttatt
     3721 attagcagtc gtggcatcca ctattcctat tgtattaata acagataacg ataataaact
     3781 gatggaaaaa acaataataa tcaagcagta atataattta taactagagt tgaacaataa
     3841 tttatttcat ttgaagatct atttaaattt ctcttaaatt tatttatgaa tacttattat
     3901 aacaatatta agaaaattct tctgcctcaa ttagcattaa cctctataat caaccattag
     3961 gccgtcaaat atcccttatc aaaataaaat gtttataaca aaattcatta gacttagtca
     4021 gttatggaca gaacttttct agggttataa taaaatttac ttcttagtca tagattcaaa
     4081 taatagtttg aagtttttaa tttacggtat tgatatagtg atgcgttttt ttttgagaag
     4141 catgtagtta taagcagaat aatgttcttt attcccattt tgaaaataat aagctaaatt
     4201 tattatccct taatgtactt gaagatttat agatttagcg cacgatagta tcattatttg
     4261 aataaaaaaa atgagattta cattatggtc ccttttattc ttgccttttt actctttagt
     4321 atatttaata ataataataa taatactttc gcgcagtcgc ctgctgccgc tccctcatct
     4381 attgaaggca ttaaagttta cgatcctaat ttaaaaatag aattggtagc taatggattt
     4441 gattttccta ccaccatggc atttttgggt cccaatgact ttttaatact agaaaaatca
     4501 ggagcggtta aacgtgttac agatggtgta gtagtaaaga agccactaat tgaggtgaat
     4561 gcaagtttga aagatgaacg aggtttatta ggaatagcta taagccagct taaaaatcct
     4621 attagtcaag tatctaccca agaagatcct aaaataactc ataatatctt tctttattat
     4681 gttgaatgta aaaaccattc attagaatgt agtaataaca tttacagata tcaaatggat
     4741 attaaaaata atgccttgat aaatccaaag ttattgttaa gtattccatc ttttcccgat
     4801 ccctctcata ttggtggtat aataagaata ggccatgatg acaatctgta tgtaacagtg
     4861 ggaagctttc agaatactgg tgttcctccg acaataaaaa acaaagcact aaactttgaa
     4921 gatggtaata actttgatgg aagagggagt attctacgta taacacagga aggcaagcct
     4981 gttaatgatg gtggtggtaa tataatagga gatacctatc cactaaattt gtattatgct
     5041 tatggaataa gaaatagttt tggactagat tttgatccat taactggtaa attatgggat
     5101 acagaaaatg gaccgtattt tggtgatgaa attaacttgg tagaaccagg attcaatagt
     5161 ggttctgata agatatttgg gatatggaaa aatgatggat ttgataacaa gcagagagaa
     5221 ggtaaaagta aaagtaagtc tgagaattat gtcattgtaa atgaaacaca tcctgatgat
     5281 cttatttata caggtaaagg acattacaat cttccaaaat ttatgtggga taagactgta
     5341 gcaccaactg cactattatt cttgaattcg aaaaccttgg gacctaatta cgaaaatgat
     5401 atgtttatag gctcagtcaa agatggaaaa ttgtttcatt ttgaattaaa caatcttcga
     5461 aacggtctaa atttggaggg tgttttatcg gataaagtag ctacaaataa agacgaattt
     5521 ggtagtatta tatttgctga gggatttaat tacataacag accttaaagt aggtccagag
     5581 gatggatatt tgtatattgt ctcaggtttt aaatcaaaac aggaaaagac agaaaaagta
     5641 ttgaaaactg gaactgtttt taggatagtg cctatcgaaa ccactattaa agaagacaat
     5701 gaggagatag aaaagccgcc tctagagaga gtatttccca aaataaatat tttgaatggc
     5761 ctgtctccca aattagaaga gactcctaaa atagacacaa atatacttaa tcaattagcc
     5821 ccaaaacctt ctaagactcc caaattagat gttaaagcat gggatgatct tatgaaacag
     5881 acacaaggag cgacaataga cgagaagatt gtaaaactca ctggtgacga acaacaacag
     5941 atgcaaagta ataatctcac tgaaaaaaat atcaatcaaa catcaaaaat cacagacgat
     6001 tttaaaccag gtgatcaaat agtacttcaa ggtagcgcgt catcagtttc aaaagaatat
     6061 ccagatgatg atgaaccaca ggcacatcag gtagcagtac ttcttcctcc aagagatgat
     6121 aaatcaatat actcaggaac tattacttta acaacgacta aacctcttga gatactctct
     6181 ttaaatgatt atcaattaaa caaaaatatt ctttctaata tgtcagagtc attcgggact
     6241 tttactacta taccttcttc tgctaatgta tcaaactctt atattgcaac aacaatagcg
     6301 actcttgaga agaaaaatga ttttggctat gatttgtcga tccctttctt agggaattcc
     6361 cttgaattac acaataccaa agatgaacca ttcattgcag tatataccgt cagagctgat
     6421 atattaaaat ccga
//
LOCUS       DASVEV010000143         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1607844, whole genome shotgun sequence.
ACCESSION   DASVEV010000143 DASVEV010000000
VERSION     DASVEV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607844"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF222_06455"
     CDS             <1..397
                     /locus_tag="VF222_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7142100.1"
                     /translation="TGTGLNTQHWRFIVVEDKDNLNKLAEDSTSGKWVTDANFAIIVL
                     TNPKYNYHMIDAGRVLQSMQLAAWNFGVGSGLFTGIREQNFRKDFSIPEEMNITIVVG
                     FGYPAKKNLTGKRKNRLPLLDLVYYEKYG"
     gene            complement(637..873)
                     /locus_tag="VF222_06460"
     CDS             complement(637..873)
                     /locus_tag="VF222_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142101.1"
                     /translation="MHLPKNTGKYLILIGLILIIAGIVFTMQSKSIVGPPSSFMYKNP
                     EWTVNGYAIITLGTIIIVVGIILWQIGMKKSIAD"
     gene            1182..2414
                     /locus_tag="VF222_06465"
     CDS             1182..2414
                     /locus_tag="VF222_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142102.1"
                     /translation="MSKSIENEESLHLSVKEAFNRGVIRGQSQSNFFEFFDFIGNPFD
                     PSFLITNQKQIVKSTRVIINKLAERIGACHKNQRHLILVGPECSGRSTILKIMENFLN
                     KGFNENFSLYVNAQDRWSGFTTAEDGNDDHFDRIDNFQQWVKEVDFQNSRIILVDDAD
                     SFISHAVQYCNAIKLDGFRVPTFIYCITNVTYSYVINNERLSKIFGDVFWLGIREEGE
                     IKKIILESTKNTLKSNTKQLTFFDDYSLDQIVNHSFGLPGLTSFFILSCLKTAYQANI
                     NKIERRLIKRVADSEGFTLAKKIVERNLNLDGTKYKILVEILRQYYIDGERAERKKII
                     SQFSNMARSTLAYHFKDLINENVIQQDRIGLRVYYTIQKPIRCALELIDLGLAEIETR
                     TESDIKEMVNIDQSIGDG"
     gene            2418..3464
                     /locus_tag="VF222_06470"
     CDS             2418..3464
                     /locus_tag="VF222_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142103.1"
                     /translation="MQTFNNQGDEIEFSSIFPFRISHISPSSSSFIKQINKENNYNYG
                     NSNFDSSFKILTEHLLKEPFHFEPNNYQSGYAIPSILNLKVVVIPYYEIIDGNITQNV
                     TGKENLGEVTKQTTTIKAQKLKYIIDASTFANSKIILVCKVKTVDQARDFLSDYLKAI
                     GFKESNDTWNFVYNESDTIMQQLLLSLYDYWPTNESQTTSIKIGGKTISANYSGRGKY
                     DLRLEEVNEEIKNRIINGDHIENITLKPPIGFTGTKLRKSVPKISINDQGVISCRANI
                     NYENFVIIKWFIEETINIIKQLNKVEQPKASYIKLEDFIIPEIAQATDSKLPICDNSL
                     KINNNEKILRGMND"
     gene            3461..>3972
                     /locus_tag="VF222_06475"
     CDS             3461..>3972
                     /locus_tag="VF222_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142104.1"
                     /translation="MINNHSNWHFNHTDILDNNNIRNVNSGCDVLDFNSSNTNLPRGN
                     IFHKDVGNDYTLTSKDVIRKINPVVLPRVSAIANIGLCERAAYNISFFGMESDNNFTA
                     EGVIGNAIHRITLRSMMEIIELLKNGSRNAINSSTNIITKKSKAKEIFIRNAERDIKI
                     NWKRFMLSNI"
BASE COUNT         1517 a          543 c          640 g         1272 t
ORIGIN      
        1 aacaggtaca ggtcttaata ctcaacactg gcgatttata gttgtagaag ataaagataa
       61 tctcaataaa ctggcagaag atagtacaag cggaaaatgg gtaacagatg caaattttgc
      121 aataatagtt cttactaacc caaagtataa ttatcatatg attgatgcag gacgagtatt
      181 gcagagtatg caattggcag catggaattt tggagtgggt tcagggttat ttacaggtat
      241 aagagaacaa aattttagaa aagactttag tattccagag gagatgaata ttactatagt
      301 agtaggtttt ggatatccag cgaaaaagaa tttgacgggt aaaagaaaga atcgcttacc
      361 ccttcttgac ctagtctatt atgaaaaata tggataaatc aaaaaaagat acgaaaagga
      421 gaattagata attactgcta tcatcatcat catcatcatc atcaatatcc attcataaaa
      481 aaactcgaaa tttccagcat tcttttccta atcttttgac catattcaaa atctctatag
      541 cagtataaat tttctggatc gattatgata tctttggcaa ttacatgaac cattttttag
      601 atattcaata attgaaagta atttaattcc catctcttag tcagctattg actttttcat
      661 tcctatttgc catagtatta tgccaactac aataattatt gtacctaagg ttattatagc
      721 ataaccattg acggtccatt caggattttt atacatgaaa gaagatggag gtccaacaat
      781 tgacttactt tgcattgtaa agactattcc ggcgataatt agtatcagtc ctataagaat
      841 tagatatttt ccggtatttt ttggaagatg catatctttt ttttaagtaa aaaaaataag
      901 agtctttgta attgtgaaaa tttaaaaaat ttcattgtta tattttaaat gattaattca
      961 tatggtatta atgaattgtc atttcaaatg gtatctccaa tctttaacaa taacgtcact
     1021 acatggacat tggcctgaat atggttaaga atgtagtata gatgtatgta aaagaaacca
     1081 tttatctaaa cattagttgt tacacaaaaa aatagactat tattatagaa atttttatat
     1141 tcatctagtt ctataaagtc aagagagtcg aaatagtcaa attgtctaaa tcaatcgaaa
     1201 atgaagaaag tctacattta tcggtcaaag aagcatttaa ccgtggcgta ataagaggac
     1261 aatcacagtc aaactttttc gaattttttg actttattgg caaccccttt gatccaagtt
     1321 ttttaataac aaatcaaaaa cagatcgtta agagtacaag agtaatcata aataaattag
     1381 ctgaaagaat aggtgcttgt cataaaaatc aaagacatct tattttagta ggtccggaat
     1441 gctcgggtag atcaactatc ctaaaaataa tggaaaattt cctaaataaa ggttttaatg
     1501 agaatttctc tctatatgtt aatgcacaag atagatggtc tggatttaca acagcggaag
     1561 atggaaatga cgatcatttt gatagaattg ataattttca acaatgggta aaagaagttg
     1621 attttcaaaa tagtagaata attcttgttg atgatgctga ttccttcatt tcacatgcag
     1681 tacagtattg taatgctata aaattagatg gattcagagt tcctactttt atatactgta
     1741 ttacgaatgt aacatattct tatgtaataa ataatgaaag actaagcaaa atatttggag
     1801 atgttttctg gcttggtata agggaagaag gagagataaa aaaaattatt ttagaatcta
     1861 caaaaaatac attaaaatca aataccaaac aattaacgtt ctttgatgat tattccttgg
     1921 accaaatagt taatcattcg tttggattac cagggttaac aagtttcttt attctatcat
     1981 gcttaaagac tgcctatcaa gccaatatta acaaaataga aagaagatta attaaaaggg
     2041 tagcagatag tgaaggattc actttagcca aaaaaatagt agagcgaaac ttaaatttgg
     2101 atggtactaa atataagatt ctagttgaaa ttctaagaca gtattatatt gacggggaaa
     2161 gagcggagcg aaaaaaaatc atatctcaat tctcaaatat ggcaagatct actcttgctt
     2221 atcatttcaa agatttgata aatgagaatg taatacagca agaccgtata ggacttaggg
     2281 tatattatac tatacaaaaa cccattagat gtgccttaga actaatagat ttgggtttag
     2341 cagaaataga gacaagaacg gaatctgata tcaaagaaat ggtgaatatt gatcaaagta
     2401 ttggagatgg ataagaattg cagactttta ataatcaagg cgatgaaata gaattttcat
     2461 ctatttttcc atttaggatt agtcatatct ctccttctag ttctagtttt attaaacaga
     2521 taaataaaga gaacaattat aattatggta attctaattt tgactcttca tttaaaatct
     2581 taacagagca tcttttaaaa gaaccttttc actttgaacc taataactat cagtcaggtt
     2641 atgctattcc ttctattcta aatctcaaag ttgtggtaat tccttattat gaaattattg
     2701 atggaaatat aactcagaat gtaactggta aagaaaactt aggtgaagta actaaacaga
     2761 ctacaaccat taaagctcaa aagttaaagt atattattga tgcaagtacc tttgcaaata
     2821 gtaaaataat tttagtttgt aaagttaaaa cagtagatca agctcgtgac tttctttccg
     2881 attatttaaa agctatagga tttaaggaaa gtaacgatac ttggaatttt gtttataatg
     2941 agtcagatac tattatgcag caattacttc tctcacttta tgattattgg cctacaaacg
     3001 aaagtcagac aacgtctata aaaataggag gtaaaacaat ctcagctaat tattccggaa
     3061 ggggaaagta cgatttaagg ttagaagaag ttaatgaaga aataaagaac cgtattatta
     3121 acggtgatca catagaaaat attacactaa aaccaccaat aggttttaca ggcactaaat
     3181 taagaaagtc cgtaccaaaa attagcataa atgatcaagg agttatcagt tgcagagcaa
     3241 atatcaacta tgaaaatttt gttataataa agtggtttat tgaagaaact atcaatataa
     3301 taaaacaact aaataaagtc gaacaaccaa aagcatcata tatcaaactt gaagacttta
     3361 ttatccccga aatagctcaa gccacagatt caaaactgcc gatttgtgac aattcattaa
     3421 aaattaacaa taatgaaaag attctcaggg gcatgaacga ttgatcaata atcattcaaa
     3481 ttggcatttc aaccatactg atatacttga caataataat atcaggaatg ttaattctgg
     3541 ttgtgatgtt cttgatttta attcttcaaa tacaaacctc cctaggggaa atatctttca
     3601 taaagatgtt ggcaatgatt atactttaac ttccaaagat gttataagaa aaataaatcc
     3661 cgtcgtactt cctagggtga gtgctatagc taatattggt ctctgtgaac gtgctgctta
     3721 taatatctct ttctttggca tggaatcgga taataatttt actgctgaag gagtaatagg
     3781 taatgcaatt catagaatca ctttaagatc tatgatggag attatagaat tactaaaaaa
     3841 tggtagtagg aatgccatca attcttctac aaatatcatc acaaaaaaat ctaaagctaa
     3901 agaaattttt attagaaatg cggaaagaga tatcaaaatc aattggaaac gttttatgct
     3961 ttcaaatata ga
//
LOCUS       DASVEV010000144        14398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1218795, whole genome shotgun sequence.
ACCESSION   DASVEV010000144 DASVEV010000000
VERSION     DASVEV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218795"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF222_06480"
     CDS             <1..666
                     /locus_tag="VF222_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142105.1"
                     /translation="VGKLPLLLWIIFSLPLIFYLIGKAPGFFSGEGLSQVDEQYRYFF
                     RLLFRIGTIAGNVLFGLAFFMAVRSLVSSKLKDYLIIAAIGDTMVGISLSTSAIQPTY
                     GVAAHSLVLLSSYLFSIGLYLSAISISQDNSLRKSIKGSMSDLIGNIGSAQMEQEIEK
                     KVLKLIQKQRKEMEEQTGELSYEVTEDDVRGYIKLVVDQRKKYTSPLDKKRFNKDGTK
                     FSS"
     gene            1024..1767
                     /locus_tag="VF222_06485"
     CDS             1024..1767
                     /locus_tag="VF222_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX7142106.1"
                     /translation="MSAENKMMFPGYSQTIENDKEGNLSINQISPCSMRIETNFKLET
                     NLNCYNDGLIVSGSGITIDLNGKTITGPGKTLEYSGIVILGNDINVKGPGTITNFQNG
                     VNVINSNTIDINSIILQNNQFGISSIDGSYIEMNENNVNLNSVGISSYSNNNLVLKNN
                     LITDNQLSGITLVNTTQSVMEENNVKGSGIGLFLDQPSNQNQINYNNLMYNTVDINNA
                     NGLAINLNGNQYYQNKCIVSEPAGLCLQS"
     gene            complement(2226..2702)
                     /locus_tag="VF222_06490"
     CDS             complement(2226..2702)
                     /locus_tag="VF222_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142107.1"
                     /translation="MQIDLYIILSSLFAGFVSTGIMTILEIPFWKRWGLTGILEWHEN
                     QILTCKFFRLASNNLHFWGIFLLHFLNGSLGGIGLLLAFILIPTLKEIPFLPLGLLYG
                     FFLWILTLIPIHKPITGIDPWNHPLGKGPAVLSLVGHTIYGITLVTFFLTFIFSIK"
     gene            complement(3000..3296)
                     /locus_tag="VF222_06495"
     CDS             complement(3000..3296)
                     /locus_tag="VF222_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142108.1"
                     /translation="MTDVQNGVNFDEYYLSCGHIQNIDKQDNSKNEESKPFDIDDKKR
                     MVEDTVNINHTTCNICGLTARTMKELADHTYHAHANKGEYNKKSSEQNIDPSPA"
     gene            complement(3562..3975)
                     /locus_tag="VF222_06500"
     CDS             complement(3562..3975)
                     /locus_tag="VF222_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7142109.1"
                     /translation="MMKDIENANYQFYNAFENVSINMMENIWSHNDNCICIHPGWEMF
                     VGWLAIRESWMTIFANTENLKFTITNTKIRLYEEIVSVITCVENIKMILNERKVHTGV
                     VATNIFEYDGNQRRWLIVHHHGSPISDYLPPTNSY"
     gene            4385..4885
                     /locus_tag="VF222_06505"
     CDS             4385..4885
                     /locus_tag="VF222_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7142110.1"
                     /translation="MSVSSIIENAFKIDFNTIFAVIFSTAIVALLIVFVIRWLGNKGM
                     GQLSTIELIIILGLGEAVGQAMVNPSQTSIPQGFTVVIIAIAIFKLYDYLASRYNKFS
                     KVIVPDPILLVKDGKVIDESMNKARISRKELESYMRLSGTDDISTIKSSHLEINGQVS
                     FVKKEV"
     gene            complement(5597..5752)
                     /locus_tag="VF222_06510"
     CDS             complement(5597..5752)
                     /locus_tag="VF222_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142111.1"
                     /translation="MKPRINPGMVAITIFITPPLYVSRIMDSANIGRKAAAATKGSAM
                     DILYGFE"
     gene            6003..6545
                     /locus_tag="VF222_06515"
     CDS             6003..6545
                     /locus_tag="VF222_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142112.1"
                     /translation="MKNQNLFIYSILAIGLISVPSLLDTVQAQVNMTNTTSSSVQNQT
                     AQTQANQTAQNSQNQTISKETQALMTLDIPVIKDQLMNAKTSLLNNNTEEALTGISGV
                     EIQLLLLPNKPSFVGDIQTIKNSIAKPDINKALDDLTKVQTDILKAETEALKAELANP
                     QLSAAQQNNDAQDEDDNENN"
     gene            complement(6889..7113)
                     /locus_tag="VF222_06520"
     CDS             complement(6889..7113)
                     /locus_tag="VF222_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142113.1"
                     /translation="MDYIIPNTKYNITIIIPMTRTIEPMTYSSLAGSEGTNLFNTNKI
                     IPIIINAIPVSNDTISISIYLKEFISLIED"
     gene            complement(7461..7685)
                     /locus_tag="VF222_06525"
     CDS             complement(7461..7685)
                     /locus_tag="VF222_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142114.1"
                     /translation="MKFFYSLLVYAVSIVITLSIIIVFVSGQSNSEELKIYTYYEGKF
                     LVHYPSNYIVESLENTFNTTKKTFATSRAL"
     gene            8130..9290
                     /locus_tag="VF222_06530"
     CDS             8130..9290
                     /locus_tag="VF222_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142115.1"
                     /translation="MKHKLFNINKINKINIILVVFSIPFFLFSIDSAYSIKNDNNEIK
                     DEEFDYIFDPGEYIDDDNEEQEKNTNTNNVNNQDNLAQQSNKSSKIISTNEDINLVNK
                     SNTENTINPTYVDQNNDDDNFSDKEKVVVKARINVANIEKKGFLSIAAFINGQEVLKN
                     VTLDELDPSKQTLNVNLAVNKETDIVKAGDRDEFFVCAYHIKDLLKEYNSLIYFDCDE
                     GDLLGPDTATPSISRLFSPNSLVYRESQNVFNNSSNKYSQLLSTDNDNINRNQQQQQI
                     EDKKDEKIKIKIYAPLEDRKNTQKLKIAVMIKGQIKSAVIDNVQEEISKIGGYTISRT
                     FTFDRDTNIGKIQIGDRYHTCVSSQDLNPPEGTECEKRIVKNIGRENVLYAR"
     gene            9829..10551
                     /locus_tag="VF222_06535"
     CDS             9829..10551
                     /locus_tag="VF222_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142116.1"
                     /translation="MKIQNLIIYSILAIGLISVPSLLDTVQAQITMTNTTSSVQNQTA
                     QTTPNQTAQSANQTAQSANQTAQSANQTAQTTANQTAQTTANQTAQTTANQTAQTTAN
                     QTAQSANQTISKETQALMTLDIPEIKDNLMDSKDALANNNTEEALTIITDIENELLVL
                     ESKPPFVDNIQKIKDSISKSDTNKALDDLTKVETDLLKAENEVFKAQLANPQLKVSQQ
                     DKDDNQDGNGDEDNQDEEDENN"
     gene            complement(10794..11801)
                     /locus_tag="VF222_06540"
     CDS             complement(10794..11801)
                     /locus_tag="VF222_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142117.1"
                     /translation="MSYSNIIETNCNKHAMLLYHSDDMRNKISINCINKGLERGQLCI
                     YASVDAYNNESSFNISNLSSKISNYQENIQDGNLRIINFKPYYESAISGDLTPFRELK
                     SELESTLSQRVADGKRNEILVIADAACSLSQHKHFGECIVLERWWQDVHDAWIKNNLN
                     ITVICPHPGIILYDGLFGKIKNKISDCHTLTIDVEEDHLNKQTSDNFSKKNKPIRVLI
                     AESDTDMGILYTEYFEFIGLEAVIVGSGNKCLNYVLQGIDNDFDMIIVDTHLRDISGI
                     EVAKKIRDGIPNQRIVFTTTYLKHEVSNVINQIGIDIEDCITKPFKLSTLLSVINQTQ
                     Q"
     gene            complement(11811..12017)
                     /locus_tag="VF222_06545"
     CDS             complement(11811..12017)
                     /locus_tag="VF222_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142118.1"
                     /translation="MISSFSYFSAYDIGKYLTPYQKLPIYNYPYNLQKNAIIGEKSDV
                     IISTEYSSIEKLAIINKILRATRH"
     gene            12721..13761
                     /locus_tag="VF222_06550"
     CDS             12721..13761
                     /locus_tag="VF222_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142119.1"
                     /translation="MTFNSVMLTLTLLIWLFLLISTTNNNNNDLFDFFPNKAYGHGLS
                     KDESLPIDISGRQIAIEGLIEPSFLNDDGNQQPTFTVRIFDKTTNDTISDINLRIIVN
                     FKNQTIIDQQFHSLDGIVSANLIPSKDSVTHEITKKEQQQPLSKNDPVQVSLTNPATI
                     KSKLLSDGGLYDIAVILEKTSKGIKIESDKQINLFISIGKTFPFVISESANSRSNNNN
                     DNGNLTLQVKTFYDEIQDFSFNNENSKISFKMPFKWDINYVNQILTLHEEILVPKSYT
                     PLSTVSSFTGTLNGMKIPTNTILIDDYSDKNNRIVHVVITTFKLKEFAKQIIEEGGNS
                     YAIFELEPIKTV"
     gene            13960..>14398
                     /locus_tag="VF222_06555"
     CDS             13960..>14398
                     /locus_tag="VF222_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142120.1"
                     /translation="MKSTIRSIILGLNVLLVLAIATSVYNSQFLLNNNSIMAYAQQTM
                     GMPPSGMGMGQQQPGPGQYTGGTISSIQNDEKDTPAWILSGIWKGALTNTDKSNQDFS
                     TPNVSNNNDNLPTATFESEIDMVMLNGSALHQHSIYNFTLADIM"
BASE COUNT         5362 a         2064 c         1985 g         4987 t
ORIGIN      
        1 gtaggcaaac tcccattgtt actttggatc attttttctc ttccattgat attttatttg
       61 attggaaagg ctccgggttt tttttctggt gaaggtttat cacaagtaga tgaacaatat
      121 cgctatttct ttagactttt atttagaatt ggcactatag ctggaaacgt tctctttggt
      181 ctcgcctttt ttatggctgt aagaagtttg gtctcatcaa aattaaaaga ttatcttatt
      241 attgccgcaa taggtgatac gatggttggt atttcacttt ctacttctgc tatacagccg
      301 acatatggag tagctgctca ttcgcttgtc ctactctctt cgtatttgtt tagtattgga
      361 ttatatcttt cagcaatatc aatatcacaa gacaactctc taagaaaatc aattaaagga
      421 tctatgagtg atttaatagg taatatagga tcggcacaga tggaacaaga aatagaaaag
      481 aaggtactaa agcttatcca aaaacaacgt aaggaaatgg aagaacagac tggtgaattg
      541 tcctatgaag ttactgagga tgacgtcaga ggttatataa aattagtagt tgatcaaaga
      601 aaaaaatata catccccatt agacaagaaa agattcaaca aagacggtac aaaattttct
      661 tcctagttac agctatcata ctgtacacat ctacatatag tctttaggaa gaattttatt
      721 accttctgta aattacgtta taatatgatt gatattagaa gtaacattca gactcttttt
      781 ggtcaaattt gtttcacaaa ccactgaatt atatagtata agaagccagt aattataatt
      841 cttatgtcat gcctatatta ttcactaatt ttaaatattt attgatataa gagatactca
      901 aagaagaatt ttataacttt tagattgaga ataaatggta attaataaat agttttattg
      961 aattattttt aacatatgaa tttaatttta attattttct tgatttcaac atcgactata
     1021 attttgtcag cagaaaataa aatgatgttt cctggttatt cacaaactat agaaaatgat
     1081 aaagaaggaa atctttcaat taatcaaata tcaccatgtt caatgagaat agagaccaat
     1141 tttaaactgg aaactaacct taactgttat aatgatggac tcattgtatc aggaagtgga
     1201 attacaattg acttaaatgg aaaaaccata acgggaccag gaaagacttt agaatatagt
     1261 ggaatagtga ttcttggaaa tgatatcaat gtaaaaggtc caggaactat aaccaatttt
     1321 cagaatggag taaatgttat taattcgaat actatagaca taaattcaat tattcttcaa
     1381 aacaatcaat ttggaatttc ttctatcgat ggctcttata ttgaaatgaa cgagaataat
     1441 gtaaacttaa attctgttgg tatttcttct tattctaaca ataatttagt tttaaaaaat
     1501 aatttgataa ctgataacca actgtcagga ataactttag ttaatactac tcaatctgta
     1561 atggaggaaa ataatgttaa aggatcaggt attggcctat ttttagacca accaagtaat
     1621 cagaatcaaa taaattataa taatttgatg tataatacag ttgatataaa caatgctaat
     1681 ggtttagcaa taaatttgaa cggtaatcaa tattatcaaa ataaatgtat tgtatcagaa
     1741 ccagcaggac tatgcttaca atcttgatta agaaggaata atcgaatttt attcttctaa
     1801 ttacaactat attgattata taatcaaatg gaatgtatcc agtaatgata acgaaacttt
     1861 caccttgttt taatacatat attttaagtt ctaatataat gaataaatat ttcttgtcat
     1921 aaggaagagt tgtttatgca aaaatatatt aaattatcca agttattctt agaaatataa
     1981 tcgaaatgca agttcccata tcatagtttt atttgattag atgtaatgct caatatcttc
     2041 tgcttaggtg tattatgtta tataataatt tgatgggtta ttttctgaaa tctttggtta
     2101 agaatataac aatgtacact gaaagtaaaa attttaatga acttactctg tcgatattat
     2161 cctcaatata gaaatcatac aaaaaaatat agtaattatg gtattataac gttaaaacta
     2221 cttttttatt taatactgaa tatgaatgtg aggaagaatg ttactaatgt tattccataa
     2281 attgtatgac cgaccagact caacacagct ggaccttttc ctaatggatg attccatgga
     2341 tctattccag taatgggttt gtggattgga ataagagtaa gtatccaaag gaaaaaacca
     2401 tataataatc ctaatggtaa gaaaggaatt tcttttagag tcggaatcaa tataaaagct
     2461 agtaatagtc caattccacc aagacttcca tttaaaaaat gtaacagaaa tatcccccaa
     2521 aagtgaagat tatttgatgc aagtctaaaa aacttgcatg taagaatctg attttcatgc
     2581 cattctagaa tacctgttaa tccccacctt ttccaaaatg gaatctccaa aattgtcata
     2641 attccagttg aaacaaaacc agcaaataaa cttgacaaaa taatatataa gtcgatttgc
     2701 aaatttaact ttcacatcca ggattaattt ctattggaaa attagttgtg acacatatac
     2761 acatttaatc tgttttacat taattatatt atattaaaat tttttgcaga aaaaaccgac
     2821 cttctggctt tatgaataaa atatatagaa gacattttcc ctcatggaca aggatacgaa
     2881 ctacccttaa agataaagta gtaaaatctg aatttccata aaaccaaaaa agattttaat
     2941 gtcttgaaat tcaaatataa ggattaagta attaaaaata tactttgatt actctcttct
     3001 cacgccggag atggatcgat attttgttca gatgattttt tattgtattc ccctttatta
     3061 gcatgtgcat gatatgtgtg atccgcaagt tctttcattg tgcgagctgt caagccacaa
     3121 atattacatg tagtatggtt gatattaaca gtatcttcta ccattctttt tttatcatca
     3181 atatcaaaag gtttgctttc ttcattcttc gaattatctt gtttatctat attttgtata
     3241 tgaccacagc ttagataata ctcatcaaaa ttaactccat tctgtacatc agtcacatgt
     3301 acatttctct tacaagtatt gcagatgatc attgagtcat tgggaggcac ataaagtaca
     3361 aggtatttct ttaatataaa gatattctca ttaataagat tctaaattac tatttttaaa
     3421 tcattatttt ttttatattt ataccttttt caaattttac cattaatctc ttttgttaga
     3481 taaataaata tgatatatta cataaggtac aaaatactat tgttatattt tatacaatgt
     3541 aaagacactt ttgaaatgat ttcaatatga atttgtaggt ggtaagtagt cggatatagg
     3601 cgagccatga tgatgcacaa ttaaccacct tctctgattt ccatcatact caaaaatatt
     3661 agttgccact acaccagtat gcaccttcct ttcattcaga atcatcttta tattctctac
     3721 gcatgtaatt acacttacaa tctcttcata taatctgatt tttgtgttgg taatggtaaa
     3781 tttaagattt tctgtattag caaatattgt catccaactt tcacggattg ctagccaacc
     3841 aacaaacatt tcccagccag gatgtataca gatacaatta tcattatgac tccatatatt
     3901 ttccatcata ttaattgaga cattttcaaa tgcattatag aattgataat tggcattttc
     3961 tatatctttc atcatacagt taattaattg gttttaacta tgaatgttgg tattaagtat
     4021 agttggaaat ttgaaggaga aatatgttat ctcttcaatc cttcgataat cttcattatc
     4081 cttaaaatcc gtaaattccc tctaatttaa tatattggat ttccaaaaaa aagatctcgt
     4141 atttattctt tacaaatttt ttggtttata ataatattat ttagtataaa atatttaaat
     4201 tataaaaaaa cttgcaaatc tcataactaa tcatataaat agaaaaattc taaaaattat
     4261 atagttaatt tcttctatca tttacctaat cttaaaaata aatcactctt aaaatattca
     4321 gctttttttt attttatatt ttatttaata taaacattat aaatggaagg tacattttag
     4381 ttatatgtcc gtatccagca ttatagaaaa tgcgttcaaa atagacttta acactatctt
     4441 tgcagttatt ttcagtacag caattgttgc acttttaatt gtatttgtaa tacgatggct
     4501 tggaaacaaa ggaatgggtc aattaagtac tatagagttg ataataatac taggattagg
     4561 agaagcagta ggccaagcca tggtaaaccc gagtcaaact tctattcctc agggatttac
     4621 agttgttatt atagcaatag caatattcaa gttgtatgat tacttggcat caagatataa
     4681 taaatttagc aaagtcatag tacctgatcc aatattgtta gtaaaagatg gtaaagttat
     4741 agatgaatca atgaataaag caagaatttc aagaaaagaa ttggaatcct atatgagatt
     4801 aagtggaact gatgatattt ctaccatcaa atcatcacat ttagaaatta atggtcaagt
     4861 gagttttgtt aaaaaagagg tatagaagta aaataagttt cagtaatatt ttttgaattc
     4921 gtctaaaagg ctatacaatt ttttatatgt aaccatttgt tttctgaatt cttattatag
     4981 ttattatagt tattgtttat tgattatatt gcataaataa tgatcacatg actacatata
     5041 ctattttaga ctctttgctt tattgtttat tgctagtgtt tctcttcttt tagggtctgt
     5101 agtagattgc tctataattt gtcaaagaat gcaattgctt caataatgac atttgaaaga
     5161 ggagtagtgt gtattgcaga tttacttttt cattaataga tgaattcttt agttgactaa
     5221 atctattcta atagtcatag taggaattat agttggtgga ctatctccta tacaatagcc
     5281 tttagacaat atcaaaaaaa gatgacatgg tgcggatcga gatgatggta agagtacttt
     5341 acggttagca tattagttgg ttggctctgt gatagagaat atcccacaga acatggcttt
     5401 agtgattctt tgataacaag aagtaccata gatataatat caattgtagc aatttttatt
     5461 tcgattagat tttttgaaga atctcgtcaa ctgtatatga aaacaaatgg aaagattaga
     5521 tagaaaataa ttcctctttg gtcaatagaa gttttcatat caagaatatc ggtggtaact
     5581 gacttgagta tcgattctac tcaaatccat atagaatatc catcgcactc ccttttgttg
     5641 cagcagctgc cttccttcct atgttagctg aatccatgat cctcgaaaca tataatggag
     5701 gagtgataaa aatagtaatt gccaccatgc ctgggtttat tcttggcttc atattgggaa
     5761 aattgaagat ggtaattaat aattctctat aatgctaaat actaaagcaa ttaattgata
     5821 tacagaagaa tagaacgatt acttaaagac atacttatat cctgtaacct tataaaagaa
     5881 tatacaaaat tttaattaaa tatatttggt ttagtaatat ttatagaacc atatcataaa
     5941 aattaaaatt gagtatttaa tcaataaaac cttataaata atatagtatc ttatcattca
     6001 gaatgaaaaa tcaaaatttg tttatctatt caattcttgc aattggatta atatctgtac
     6061 catccttgct cgatacagtg caagcacaag tcaatatgac aaacactact agttcatcag
     6121 tacaaaacca aactgctcaa acacaagcga accaaactgc acagaattca caaaatcaaa
     6181 caatcagtaa agaaacccaa gcattaatga cattagatat acctgtgatt aaagatcagt
     6241 taatgaatgc aaagacatca cttcttaata ataatacaga agaagcatta acaggaattt
     6301 caggtgtaga aatccaattg ttattgttac caaacaagcc atcttttgtt ggcgacattc
     6361 aaacaattaa aaattctatc gctaaaccag atataaataa ggcattagat gatctcacca
     6421 aggttcaaac tgatattcta aaggcagaga ctgaggcatt aaaagccgaa ctagccaatc
     6481 cacagttgtc ggctgcacaa caaaataatg atgctcaaga cgaagatgat aatgaaaaca
     6541 actaacaata caatccaaca ttttttttat tttgattgtc gcttatacct tgaccataga
     6601 ttttaatcgc cattagcacc attaactagc tgatatagta atatcgcaaa tattttataa
     6661 ttttgtctct ttaatacaaa taggtataat caattatttc cttgttgcag ttcactatta
     6721 gtgcttaaca cttatggatt acagaaagat ggatgtaaat ttcaaaaatg tagagatgac
     6781 acaaatctat gaccttatgt atatctgtgc tacgtacgct gatggatcat tccttattta
     6841 cagtgcctaa aataatgttt agataaatta tagctaaatt tctttctttt aatcttcaat
     6901 aagggatata aactccttta gatatataga aatagaaatg gtatcgttag aaacaggaat
     6961 tgcattaatt ataattggaa taattttgtt agtattgaat aggttcgttc cctcgcttcc
     7021 agcaagacta ctgtacgtca taggctcgat tgttctagtc ataggaataa ttatggttat
     7081 attatatttt gtatttggaa ttatataatc catataatat aacaagaatt ttttttattt
     7141 gtacatttat agttacagta atgaaatgga gatttttttg tttgttagat attgaatcga
     7201 aataacctct atagataaat ttaatatgca tccttcttca tcatggtttt gaaaagtaga
     7261 aggtcaaatg aatttcaagg caaaattcct tcgtaataga tctactcatt gttcttacaa
     7321 atgattagaa gagattggac cataattgga gtattattcg gataagagtt tattgattta
     7381 aggtggatat caatcacata gacaggttaa aacatcttaa ctatatgatt aattacaaat
     7441 aactggtcct ctcactaaat ctataatgct ctagatgtgg caaatgtttt tttagtcgta
     7501 ttaaacgtat tctctagcga ttccacgata tagttagaag gataatgaac tagaaatttt
     7561 ccctcatagt atgtatagat ttttaattcc tcggagttag attgtccaga gacaaatact
     7621 ataatgattg ataaagtaat gacaatagaa acagcataaa ctaataaaga atagaaaaat
     7681 ttcatgtaaa tatcttctat ttattcttct tttaaatgtt attttttgct tatctaatat
     7741 agtaagttta cataagaaaa ggcttcagaa tgaccttaat tttacggata gagtataata
     7801 aatttcacaa gaaatcattg aaataatttt atagatatta ttaaataaag aatatataaa
     7861 tgatatattc tgtttattta actttttaga ctttatatta tatagaatta tatctaaaga
     7921 ttacaccaca tatttttttt tatatattta ttagttataa aaagtaataa gtaaagataa
     7981 atgagaggga gaaattatta ttcactagat ttcatcaagt tatttacaat ctgtatagtc
     8041 actcatttac attttaaatt aaatatattt tattttcgac tatcggaata ttattatttt
     8101 ttattaaaaa taatggagga aacaattaat tgaagcataa attatttaac atcaataaaa
     8161 tcaataaaat aaatatcata ttagttgtat ttagtattcc attcttttta ttttcaattg
     8221 actcagcata ttcaataaag aacgataata atgagattaa agatgaagaa tttgactata
     8281 tctttgatcc tggcgaatac atcgatgatg acaatgaaga acaagaaaaa aataccaata
     8341 ccaacaatgt aaataatcaa gataatttgg ctcaacagtc caataaatct tcgaaaataa
     8401 ttagtaccaa cgaagatatt aacctggtaa ataaatcaaa tactgaaaac actataaatc
     8461 ctacttatgt agatcaaaat aatgatgatg ataatttcag tgacaaggaa aaagtagtag
     8521 ttaaagcacg tataaatgtt gcaaacattg aaaaaaaagg gtttttaagt atagctgctt
     8581 ttatcaatgg acaagaagta ctaaaaaatg tgacattaga tgaattggat ccatcaaagc
     8641 aaacactcaa tgttaattta gcagtaaata aggaaacaga tattgtaaaa gcaggtgatc
     8701 gtgacgaatt ttttgtatgt gcataccata tcaaagattt gctaaaagaa tataattctt
     8761 tgatttattt tgattgtgat gaaggtgatc tcttgggtcc agatacagct acaccttcta
     8821 tatccaggtt attttctccc aattctcttg tttatcgaga aagtcaaaat gtcttcaaca
     8881 atagtagtaa caaatattcc caactgctct ctacagataa tgataacatt aatcgaaacc
     8941 aacaacaaca acaaatagaa gataaaaaag acgaaaaaat aaaaatcaaa atttatgctc
     9001 ctctcgaaga cagaaaaaat acacagaaat taaaaatagc tgttatgata aaaggccaaa
     9061 ttaaatcagc agttattgat aatgttcaag aagaaattag caaaattggt ggatatacaa
     9121 taagcagaac atttacattt gatagagata ccaatatagg aaaaatccaa ataggcgata
     9181 gataccatac ttgtgtttca agtcaagatt taaatcctcc tgaaggtaca gaatgtgaaa
     9241 aaaggattgt aaagaatatt ggaagagaaa atgtgttata cgctcgataa attaataaat
     9301 aattttatgt tattatatgt ttagaaatat cacattatat aataaaaaat agatcataat
     9361 agatttatac tctgaaaaat acaaactagg taattcggtt tcctttaaca taaatatttc
     9421 taaaatcatc aatgacgata ctatgtcgaa acacaaatgt tcatcattcg tgcttttaga
     9481 ttccaatgtt ttggagaaaa atacttacct ccataaatga tagcggtatc catttttcag
     9541 tactgccaat attatataaa aagaaagcct agcaaaagag gaactaattc tggagtttaa
     9601 taaggttagt ttgattctga taacaataca atactgtgtc ataataatat attaaatcat
     9661 acttcgggca acatctatta caatgatgag ttagtaaata ctaatattat ttttgttgga
     9721 taatatgtaa taaaatcatt gagtaaaatc tttacattac tttattaaat tattcattac
     9781 tataaaaata acaagtttta taaatactag tgctttttat ttaataaatt gaaaattcaa
     9841 aatttgatta tctattcaat tcttgcaatt ggattaatat ctgtaccatc attgctcgat
     9901 acagtgcaag cacaaatcac tatgacaaac actactagtt cagtacaaaa ccaaacggca
     9961 caaacaacac ccaatcaaac tgctcaaagt gctaatcaaa ctgctcaaag tgctaatcaa
    10021 actgctcaaa gtgctaatca aactgctcaa acaacagcga accaaactgc tcaaacaaca
    10081 gcgaaccaaa ctgctcaaac aacagcgaac caaactgctc aaacaacagc gaaccaaact
    10141 gctcaaagtg ctaatcaaac catcagtaaa gaaacccaag cattaatgac actagatatt
    10201 ccagagatta aagataattt aatggattca aaggatgcac tcgctaacaa taatacagaa
    10261 gaagcattaa caataattac ggacatagaa aatgaactct tggtgttaga aagtaagcca
    10321 ccttttgtag acaacataca aaaaattaaa gattctattt ctaaatcaga tacaaataag
    10381 gcattagatg atcttaccaa ggtcgaaact gatcttctaa aggcagagaa tgaagttttc
    10441 aaagcccaac tagccaatcc acaattaaag gtttcacaac aagataagga cgataatcaa
    10501 gacgggaacg gcgacgaaga taatcaagac gaagaagacg aaaataacta acaattctta
    10561 atttttttta tgtatactga attagaaaca taggataatt tgcaaataga gaaattatta
    10621 cgttttatca aaatagttgt tttattaaaa tcacgatttt cacattgatt atcaaattga
    10681 ttaatacttg ttcatacttt atgtataatt gaaatcgtat taaaaatagt tgttatttaa
    10741 aaattaatta aataataaaa gagtatattt taagctatat caacaaattt ctcttattgt
    10801 tgagtttggt ttattacaga gagtaatgtc gataatttga aaggtttcgt aatacaatct
    10861 tctatatcaa ttccaatttg atttataaca ttgcttacct catgttttag ataggtggtg
    10921 gtgaacacaa ttctttgatt aggtatacca tctcgaattt tttttgcaac ttctatacca
    10981 gaaatatctc gcaggtgtgt atctacgata atcatatcaa agtcattgtc tattccctgt
    11041 aaaacatagt ttaaacattt attaccagat cctactatga cagcttccaa tcctataaat
    11101 tcaaaatatt cagtatacag aattcccatg tcagtatcag attctgcaat aagtactcgt
    11161 ataggtttgt ttttcttact aaaattgtct gatgtttgct tgtttagatg atcttcttct
    11221 acatcaattg ttagagtatg acaatcactg attttgttct ttatttttcc aaataatcca
    11281 tcataaagga taattcctgg gtgaggacat attacagtta tgtttagatt attttttatc
    11341 caggcatcat gaacatcttg ccaccatctt tctaatacga tacactcccc aaagtgttta
    11401 tgttgagata atgaacatgc agcatctgca atcaccaata tttcatttct tttaccatca
    11461 gccacacgct gactcaaagt agattctaat tcacttttta gttctctaaa tggagtcaaa
    11521 tctccagata tagcagattc ataataaggt ttgaaattga taattcgtaa attaccatct
    11581 tgaatattct cttgatagtt tgatatttta gaagaaagat tggatatgtt aaaactgctt
    11641 tcattattgt atgcatcaac ggatgcataa atgcagagtt gacctctttc tagtccttta
    11701 ttgatacaat ttattgaaat tttattacgc atatcatcgc tatggtataa taacatagca
    11761 tgtttgttac aatttgtttc aattatattt gaatatgaca ttttatctag ttaatgcctc
    11821 gtcgctctga gtattttgtt tataatagcc aatttttcaa tagagctata ttctgtagaa
    11881 ataattacat cagatttttc cccgattata gcatttttct ggagattata aggataatta
    11941 taaataggta atttttgata aggagttaaa tatttcccaa tatcatatgc actaaaatat
    12001 gaaaatgagc taatcatcat ataaacaata tttatgattt aacaagatat atatgcattt
    12061 agaatcattt ttaacaaatt cgaatagagt tttttcataa aagtacaatc ttctcaaatt
    12121 tggtttattt atttatttat cttttcttaa ttaaattctc tccaacttta tctttatcgc
    12181 attcaaatga cattgttata gatcttaagt tactttatat aggatgaaat tattctccaa
    12241 tttctttatt ataaaattta tctttagaca tttatttatc atccatgcag agttaaataa
    12301 aaggtgttaa aaacagataa aaagtagtat tataattcat tcatcaataa ttaaaggatt
    12361 taagattatc actatcgggt taaaatttac tttctaacaa atacactaaa ccgttaaata
    12421 tagatagatt aagaattagt aaatggtgtt aaaaaaacaa ctacaaattt attattctaa
    12481 tgcatgttta tacctttaat tgttaaaatg ttctccattt ttgtaataaa tacagctctt
    12541 tactttgcaa tgtgtaccca gctattccct tataattaat acctacttaa cacctttttg
    12601 tatgcatcaa taaaaccaat gaaatctttt accactacta ttcaactgat acgatcataa
    12661 tcaaatttaa aataatattt ccgtaacatt ttattttgag ctgtgattat atttttctct
    12721 atgacattta attctgttat gcttactttg acgttgctaa tatggttatt tttattgata
    12781 tctactacta ataataataa taatgatttg tttgattttt ttccaaacaa agcatatgga
    12841 cacggtttga gtaaagacga atctcttcct attgatattt ctggaagaca aattgcaata
    12901 gaaggtttaa tagaaccatc gttcttaaat gatgatggta atcagcaacc tacgttcaca
    12961 gttcgtatct ttgataaaac aactaatgat actataagcg atattaatct tagaataatt
    13021 gtaaacttta agaatcagac tatcatagac caacaatttc attccttaga cggaatcgtt
    13081 tcagcaaacc ttattccttc taaggattcc gtaactcatg aaataacaaa gaaagaacaa
    13141 caacagcctt tatcaaaaaa tgatccagtg caagtaagtt taacaaatcc agctacaatt
    13201 aaaagtaaac ttctctctga cggtggatta tacgatatag cagttattct tgaaaaaaca
    13261 agtaaaggaa ttaaaataga atcggataaa caaattaatt tatttataag tataggaaaa
    13321 acttttcctt ttgtaataag cgaatctgca aatagtagaa gcaataacaa taatgataat
    13381 ggtaacctta ctttacaagt taaaaccttt tacgatgaga tccaagattt ttcctttaat
    13441 aatgaaaatt caaaaatttc atttaaaatg ccttttaaat gggatataaa ttatgtaaac
    13501 cagattctaa ctttacacga agagatatta gtcccaaaat cttatacacc attatctaca
    13561 gtttcatcat ttacaggtac tctgaatggt atgaaaattc ctacaaatac aatattaata
    13621 gatgattatt ctgataaaaa taataggatt gtacatgttg ttattacaac ttttaaatta
    13681 aaagaatttg ctaagcaaat tatagaagaa ggaggaaatt catatgctat atttgaattg
    13741 gaaccgatta aaacagtata agtaatatca atattacaac tttccatacc acaaaaagtt
    13801 gaaaaacatt cattgctgcc tagttaatac atataagata atcgtagttt gtataaaaaa
    13861 aatagatggg taaagacaca actaactatg atttttactt acaaatcact ttaaaaaatc
    13921 taaattatta tatataacgt atattctttg tttattacat tgaaatctac tattagatcg
    13981 attattttag gactaaatgt tttattggtc cttgcaatag caacttctgt ctataattct
    14041 caatttctct tgaataataa cagtataatg gcatatgcac aacaaactat gggaatgcct
    14101 ccgagtggaa tgggaatggg acaacaacaa ccaggcccag gccaatatac tggaggaaca
    14161 atttccagta ttcaaaatga tgaaaaagat acacctgctt ggatattatc cggcatatgg
    14221 aaaggtgctt taactaatac tgataaatca aatcaagatt tctcgacacc gaatgttagt
    14281 aataataacg ataaccttcc aaccgctacc tttgagtcag aaattgatat ggtgatgcta
    14341 aatggatctg cattgcatca acatagtatc tataacttta cacttgctga cattatga
//
LOCUS       DASVEV010000145        24503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_830499, whole genome shotgun sequence.
ACCESSION   DASVEV010000145 DASVEV010000000
VERSION     DASVEV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 24503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24503
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830499"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF222_06560"
     CDS             complement(<1..630)
                     /locus_tag="VF222_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7142121.1"
                     /translation="MSLKFDLFNILIGKENDSDYKNKPVIIQGMTGSFGSTHTKLMKD
                     YGTNIVAGVTPGKGGQSFEDIPVYNTVAEAVNNNSIEISGIFVPAPFFLSAAKEAIDN
                     GIKLLVAVPEHVPIIDSIKILEYAKKRGARMIGPNTPGVIVPEIMKVGIMPAKPFTKG
                     STVVFSRSGTLMYEVSYTLSNAGYGQRFCLGLGGDPINGTNLIEAFELIK"
     gene            complement(649..1800)
                     /locus_tag="VF222_06565"
     CDS             complement(649..1800)
                     /locus_tag="VF222_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEX7142122.1"
                     /translation="MLKNKNIKAQKINLLVKMKLLEYQGKELFKNYGIKVPNSYLARN
                     IEEAKRGSERIGFPFVLKSQLTVGGRGKAGAILKCKNEKELIEKFEELINKEVNGEKP
                     RTILLEEMVDIKREFYLSIFLNRTKRCYSIIASSQGGIEIENVDNNIIIDIPDVNGIS
                     SELSSEVADKMNLNNSEKNTFIDLIQKLSKLIYEKEAELAEINPLAMLDGGKFVALDA
                     KVIIDDNALFRHDDIKKYEELSELESQANKNGFSFVELDGDMAIIGNGAGLVMSTLDM
                     VSDAGGKAGAFLDFGGRATTESIYQALKVISKVDKIRAILVNLFGGIVRTDLVAQAIL
                     NAYNDGTIKVPVFARITGAESEKARELLKNSKAGLFNTVEEAINAAVKY"
     gene            2381..3424
                     /locus_tag="VF222_06570"
     CDS             2381..3424
                     /locus_tag="VF222_06570"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7142123.1"
                     /translation="MDLESKLSLITSEPTEEIVTINELRNLLQTISRPKHYIGLEISG
                     KLHLGSLILPGFKINDLIKANFNTTVFLADWHTFINNKLEGNWTKIDELSKYYSEAFR
                     FFCPGVNIILGSELYVEFEDYWKNFMQFCKHITLSRTMRSLTIMGRNANENLDFSQFL
                     YPAMQSTDIHALDLDLVHSGTDQRKIHMLMREVFPKLKWKVPISLHHHLLPGLSEPVK
                     LGLTEDSSEDLRISSKMSKSKPMSGIFIHDDDKTIETKIKKSFCPIGVSENNPILEII
                     RYIIFHRFNDFIIERPSKYGGTISYGNYSDLKEDYDKQKIHPQDIKQSTSSYLCKLIE
                     PIRNHFKDNEPLL"
     gene            complement(3505..4281)
                     /gene="cobJ"
                     /locus_tag="VF222_06575"
     CDS             complement(3505..4281)
                     /gene="cobJ"
                     /locus_tag="VF222_06575"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX7142124.1"
                     /translation="MSKGKLYVVGVGPGNHDHMTYRAKQVIEESEIIVGYDTYVSLVE
                     DLIEGKEVYRYAMTQEVDRANQAIEFAENGKIVSLVSSGDPGIYGMIGLIYEILAEKG
                     WKKESGMYVECVPGVSSLNSCGALVGSPLMTDFAVVSMSDLLVPWEMIVKRVEAAAIG
                     DYVTVIYNPASKKRIHQLRDTRDILLKYRKPDTPVAIIKGAYRDSQEIVLTNLQDMLN
                     YSDMIGMITTIIIGNSSTYKYNNMMINPRGYKSKYQLVKE"
     gene            complement(4330..5730)
                     /locus_tag="VF222_06580"
     CDS             complement(4330..5730)
                     /locus_tag="VF222_06580"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7142125.1"
                     /translation="MRIVMKFGGSLLDNEKKILNVVNIIKSYYYLDYEIVVVVSALYG
                     ITDKISELCDAIKKINRNSLELFLKDMENVHINLVEKVIHKEKDKHELLYLLKDLLNE
                     FRDVLLGISILGDIIPKYRDYLFSFGERLSTPIVSFALKSAGINSVYMNGKEVGILTD
                     SNFGDAKPLMDTTKLRVHHKIEPLIKQRIIPVVTGFIGCDQNGNTTTIGRGGSDYSAT
                     IIAASINANEVWLLGDVNGLMTADIKIVKNARVLEEISFSEALELSMFGSKYMHPRAL
                     EPVMDSKIAVRIRNANNIDHPGTLITQIPTLAYNKIVKSITVIRNTALIDVSGGGLIG
                     SPGTAAKIFDALAKNHVNIMMISQTPSESSITIVVKKQDLDKAIMTLELNFLGKLIKR
                     IDVSENVAVIAVVGSGMRGIKGVAAKTFGAVAKRDINVIMITQGSSELNLAFVIDDKN
                     CEEAVNILHQEFINIK"
     gene            complement(5817..6569)
                     /gene="pcn"
                     /locus_tag="VF222_06585"
     CDS             complement(5817..6569)
                     /gene="pcn"
                     /locus_tag="VF222_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HEX7142126.1"
                     /translation="MTFLAKTKTPDEWKAITSAISTLVDEATFEASVEGISFRGMDPS
                     HVALIDIYWPNSSFEKYECDSLIKFGVRIDEFSKLIKRSEKRDSIELSINKESMLILK
                     IQNGYKKEYKMRLIESASSSTPLPKLNFNSKIILTASAFDKILSDIQVLSEYITIDCK
                     SENIRFLGRGDSGEADIFLEPKNEGLEELTSTEDSLGTYSIDYLIKITKAISAVGGSV
                     IAEYSSKMPLRLEFTVANLGRIHFYLAPRIQD"
     gene            complement(6660..6989)
                     /locus_tag="VF222_06590"
     CDS             complement(6660..6989)
                     /locus_tag="VF222_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HEX7142127.1"
                     /translation="MKFCPHCETRMRLKFEENQVACPKCGFTSSQQKSTVTVNVGNIT
                     TQEQSALRILDGENTTQTLPTISIDCPKCNNNLAVWWMLQTRSADEATTQFYRCTKCS
                     HTWRNYS"
     gene            complement(6991..7314)
                     /locus_tag="VF222_06595"
     CDS             complement(6991..7314)
                     /locus_tag="VF222_06595"
                     /inference="COORDINATES: protein motif:HMM:NF025040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HEX7142128.1"
                     /translation="MWYDLYPKYVLDYLMQAEITTFSNNEIELKLKDEDISIMYIIQH
                     HILKEKDIEFAGIVMKHPLIKEYLMKIKSKENPMDILDKSIKSADEFLKDISHLIESS
                     EQKVL"
     gene            7327..7848
                     /locus_tag="VF222_06600"
     CDS             7327..7848
                     /locus_tag="VF222_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142129.1"
                     /translation="MTEKINSLDNIVLTNITSKTNSKEQLSFNLSLKINGKIRDLFTQ
                     SLWEKAYNKFDNTFRITINVDLKYKSKVIYSVKFVRKAVFFWTRNPKIHHRIWIFIVK
                     DDSPFYPESEDEAKSLLFDIDNIVEIKLPAIIDEVSTLYANVAVSWGKHNFTDPTKLV
                     GKSNEEEIHVVNS"
     gene            7921..8067
                     /locus_tag="VF222_06605"
     CDS             7921..8067
                     /locus_tag="VF222_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142130.1"
                     /translation="MAKFDGIKGQELLDIEIQGNELTFIFKDNRYLFVKNEKDKMICD
                     SLPE"
     gene            8390..9727
                     /gene="asnS"
                     /locus_tag="VF222_06610"
     CDS             8390..9727
                     /gene="asnS"
                     /locus_tag="VF222_06610"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX7142131.1"
                     /translation="MNLNIKKDSIMLNIRTAELKKDDYIGKYISIKGWIHRIRKQKEN
                     TFLLIRDDRGGIIQCIIESSKTNNLTIESSVEIFGILQKDSRAPEGGYELKADRLNIF
                     SISKNDYPIGEYQSLDLLLEKRHLTIRTRRMTMVSKIRSSILYFSRKWFTDNDWIEVT
                     APVIVKGSVEGGSTLFKIKYFDEDAYLSQSAQLYLEAMIFSLGPVWSMTPSFRAEKSR
                     TVRHLTEFLHLEAEAPWISLEDLIKLQEELISFVIQNILKNNKEEFDFLKRDVSDLEK
                     ISPPFDRITYEKAIDKLRKEEFKIIEKGSSRKIEYGDDLSIESERILTINSSKPLFVI
                     GYPLQIKPFYVKEEISKQGLGIAADLLAPNGFGEVTSGGLREDNLQKLENRMVLEGLQ
                     KSNYDWYLDLRRYGSVPHGGFGLGIERLMRWILDITDIKDTLPFPRTIRRIYP"
     gene            9837..10643
                     /locus_tag="VF222_06615"
     CDS             9837..10643
                     /locus_tag="VF222_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7142132.1"
                     /translation="MHNNLPEKTESLFFELAGDLRLSMLLKLSKHKYRLSQLSTELDA
                     TMQEAHRNIIRLVESGLVFKDGEGDLGLTSYGKSIVFLIPGYEFLLQNKQYFIEHSIE
                     ELPLKFIQRIGSLQNSEIVLGVMANLQRWKTLYWNSQVYIKEIMSQVPLDLIETVSNK
                     IQTGIKFSYIFSKDTIIPKGRGELLQKIGWVNQISKGMVERKMINKVTIMTIFNEQEA
                     CLLFPNLKGEPDLNIMFYSKDRNFHEWCKDFFDYQWEMAGPFDEKKLNET"
     gene            10701..10931
                     /locus_tag="VF222_06620"
     CDS             10701..10931
                     /locus_tag="VF222_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7142133.1"
                     /translation="MPGAFVLLNTELGSEDSIVKELRNLEGVKEVFQVYGVYDIIAKV
                     ESDTMDKVKEIITWKLRKLPGVKSTLTMIVME"
     gene            11018..11188
                     /locus_tag="VF222_06625"
     CDS             11018..11188
                     /locus_tag="VF222_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142134.1"
                     /translation="MGFSNILKRKKGKEANEIKCKNCNIVFQDKEHLSRHNKKAHGKN
                     DDFLPSSNPFQV"
     gene            11217..12218
                     /locus_tag="VF222_06630"
     CDS             11217..12218
                     /locus_tag="VF222_06630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7142135.1"
                     /translation="MFPIHDDTPRLHGRPYVNYSLIALNVIIFIWEVISTNYFSNELK
                     VNEIFFTYGTVPDRVFQGDYFTLITSMFLHGGVVHIIGNMVFLYIFGDNIEDRFGHFK
                     YLILYLMWGIAAGVIHSFYAVSVGGGNIPAVGASGAISGVLGAYLVLFPRAKIFTVIT
                     AFFITTVRIPALAYIPVWFILQVVFSIFNPEGGVAYLAHIGGFVVGAGVSFLAKSFFP
                     SVFQGHIGVSSIYSKKSSKPYSRPYNQDNTRHSISQPDIIEGNNYYEILVELKGLRNI
                     QNLRAIYEKSTHSLQIFSEGAPKPIVSIPLREDMHISQIQDVSYINGILRIRMLKVN"
     gene            12306..12917
                     /locus_tag="VF222_06635"
     CDS             12306..12917
                     /locus_tag="VF222_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7142136.1"
                     /translation="MSEQPQARGNDKISRRDFLKLLAAAGTVLTFTPFVDWGKFLPNP
                     SGSTTEKSKVQLPDGSQANVKTFKVNHSEAIIYPISEDAALNKEAFRTWQFIRLPPEL
                     GGEKNDASAFRVFSMVCLHLWCLWKYWPGEGRKRGECPCHGSLYDPLTGAAFAGPAAL
                     QSPPSNVLPKLDLEADKDGYLWILPPNWNVNGNGIVGYGRFIA"
     gene            12979..14451
                     /locus_tag="VF222_06640"
     CDS             12979..14451
                     /locus_tag="VF222_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7142137.1"
                     /translation="MGLKFTFPTRFVSPLGFLGMLTFVLFIILGISGALLMLWYEPIL
                     DRAWDSVENINDTIPYGFHLRNIHYHASNAMVMMAILHMYYQYFSGRYKIRNEILWVT
                     GILIGVLTILEAFTGYDIIFSERAELAISIAASLTNSIPIIGPDMRDAFFGSGFHDFV
                     LRFYAFHVFLLPIALIGLMTVHFPRFLVFDVPMVMAITGAIMITGGVFPVDMGLKFDP
                     NVPPGITVPEWYLTGLYAFLRSQYDKFVTGVAWPGLFIFTLLIIPFIDKYKKFSWKDR
                     PLISAIGITSIAQIVVTTYWGFYIDPDRTKSLLDRLVIDPIFLYSVMILLVPLSFGFT
                     YLMIKLAKNAEANAKRQPRKKESVISLPEKWILFVFIALIGFQIYLNIAAYYALLNGM
                     KNISLFIIGIMMLVFAGMFHIYRHGRSNANNPPPPSIPQTDRIKSLQYKKRALVESDN
                     DKPGLESSENSIGQIKSNGILNKVFKVARKNKNSLEKRWK"
     gene            14521..14997
                     /locus_tag="VF222_06645"
     CDS             14521..14997
                     /locus_tag="VF222_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7142138.1"
                     /translation="MERSQQFSSIGGLTIIFFIIAITISLAFYQFVFLKELNLKPSVP
                     EEVANPPQPLKLTIAEGASLPTNPQFYVPSIARGSLGIDNKLIWENLDTTPHTVTTDN
                     DYHDPLSGKFDSMETIGLVPPGGTFEFVFTEDGEYPYHCEPHPWMTGTVTIVKNFS"
     gene            complement(15009..15377)
                     /locus_tag="VF222_06650"
     CDS             complement(15009..15377)
                     /locus_tag="VF222_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142139.1"
                     /translation="MQKKKELSLTIEENEIHPNQEIHGSIEVNYNGRFDTIVINSQIE
                     NSNDICNYIELNGKKINHPYARLSIYKKELEEKRIIDFIAITQHVPKNFFSKVKFRTS
                     IIQEHKEVANDILFLQIKKV"
     gene            complement(16300..16449)
                     /locus_tag="VF222_06655"
     CDS             complement(16300..16449)
                     /locus_tag="VF222_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142140.1"
                     /translation="MYQTLLLAKKTSKDDYTTHMRFVLLGMGVVGAIGFTIKLIGEFL
                     TNKPV"
     gene            complement(16580..17017)
                     /locus_tag="VF222_06660"
     CDS             complement(16580..17017)
                     /locus_tag="VF222_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7142141.1"
                     /translation="MKILTELTRDASISVPQLSKNLNINSSVLYSRIKRLSKRNLIKK
                     FTIMVNENMLGLNIKATVGINRDPKRKDHIHKELLTIAEVRSLSEVTGRFDMIVNVNA
                     STLEDLHNIVIERIGKIEGIQNTETFVEMQRTEKDPDYSEQVY"
     gene            17235..17738
                     /locus_tag="VF222_06665"
     CDS             17235..17738
                     /locus_tag="VF222_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142142.1"
                     /translation="MFGLGKIKTGDYVFAVEPESESKRIILGRVTSVNNNSLLILGSY
                     IKPLGLLSKRQKGLLGKRSQEVLNDPDPNNCIFILIDRIETGNFYGNIDQSINKVSWI
                     NEKRFFVLEGWIKENMADLFADVIYSQSEDEKHHFRAILIEKMNSLYDRELKEHVYAV
                     ARGAKIL"
     gene            17791..18108
                     /gene="rpl12p"
                     /locus_tag="VF222_06670"
     CDS             17791..18108
                     /gene="rpl12p"
                     /locus_tag="VF222_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HEX7142143.1"
                     /translation="MEYVYAALLLHKLKQDITEDNVTNVIKATGSAPDTVRIKALVAA
                     LGDVDIEAALKAAPMTMATAAAAPPATGAAATETTTKEEPEEEKSEKKEEEALEGLSS
                     LFG"
     gene            complement(18381..19454)
                     /locus_tag="VF222_06675"
     CDS             complement(18381..19454)
                     /locus_tag="VF222_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142144.1"
                     /translation="MAENNIFTMLFWLGELTLASWILSYGAEHLSEKFGAKFVGRVLL
                     SIATTLPEIAIVIYAAAQGSFGIAIGSGLGSNLLMMTLGLAIMLIIATTRLSKAPLKH
                     VDVSTFKNDMIFLIVAAVISFVLFLDGFNYLDGFIFVGLFAGYIILSLHEMRVENKDK
                     VSDKNVSLNISNNNNNNNKKLIVEQNISNDNNDNKKFIKAIGTFLLGAIGILFGAEPF
                     IHSLEGVSVEFGISAVVLAVIISPIAGEMPEKISMMILARKGAAGASIAIANVLGSKI
                     LNNTLLLAVAVFAAMYSFGFNKVIELTEILYYQIILVTIVTIVAVLPMMFRKKIGLRF
                     GFVLLSMYIICILIQFLFPQNMH"
     gene            complement(19562..19705)
                     /locus_tag="VF222_06680"
     CDS             complement(19562..19705)
                     /locus_tag="VF222_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142145.1"
                     /translation="MVTCAAVPVFLVILTAPHPTQFITVMEEGFLDIQPYNVITKFPF
                     IQS"
     gene            19896..20564
                     /locus_tag="VF222_06685"
     CDS             19896..20564
                     /locus_tag="VF222_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7142146.1"
                     /translation="MTSDVELKDLIEHARRDYKKRNFDQSLDLTIVLKDIDVKKGFSF
                     NEVVHLPNKPSRQATVCVVASGEMGSRAKRAEADRIIELDELDRLGTNKKEAKKLAKA
                     YDFFLSDTSLMATVGRSLGQVLGPKGKMPTPIPYGAPIESILNRLRGSVRIRTRNQLN
                     ISTKIGDEKMDDKQLIQNANTILSLLEKKLPQGDKNIRNTSVKFTMGNCISLASLSNK
                     TKGG"
     gene            20568..21449
                     /locus_tag="VF222_06690"
     CDS             20568..21449
                     /locus_tag="VF222_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7142147.1"
                     /translation="MSSDQIIKKYPQKKQHMYQELQELPTKYNVIALSKMNKVRATQL
                     MPLRKKFHGQINIKIIKNKVAQRAFEKVSKISGIEDLSKELEGQCALMFTNISPFKLN
                     LIFSENKVFLPAKGGDIAPKDIIVPAGNTGIPPGPVLSEFKESKVQTKIDQGSIAVTK
                     DTIVVKSGEVISQKLASLLSKLDIKPIEAGVLVNFAISEGLQFREKDLQLDVKEYVKQ
                     LQISFSSALNLAIEIVYFAPETISSLLSLAYQKSLNVASKAGYVSKDTAEIILNNAQT
                     NAHGLVDALKVKGYVPQ"
     gene            21547..23181
                     /locus_tag="VF222_06695"
     CDS             21547..23181
                     /locus_tag="VF222_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7142148.1"
                     /translation="MSIQLKVHPSFFKEFATKTWVSPYEIVKELVENAFDEDATQVLI
                     TILTSGSIIIEDDAGMDVEGIEKFLLLGSPHKKIESISPKLKRSRTGRYGTGRLSFLT
                     SFESMKIKTKKNNYNKSLVIDENSLNQLFSGNSLLNELNEKPLSRNGTELNLINAKMN
                     IDPLKLYKEIRKLAILRHPMFQVFIKQSDRFTEWDLEKSDIVKAQEIQGYRIPVNLDN
                     GKIIGEIVVARRPLIEEEKGIGVMIGNHIVVRSNFGFDAKLNRITGLLRCDALTSRFA
                     DKSALIEDDAYYNFNTYVKQFIVDEVIPKLTEYEDVLITKEETRIYREIDKVLGQAVI
                     ENLESVELEEEEYETVEEKVTVPNADRLTINSSITKVKIQDNKIPTDEDYENNLSVQS
                     SKIREQAHTSILDHQTIHSEIEPHYFNPNERTQTIPNLQTSNNLDKMEIKKIKTLKRT
                     FALKKIGYKVIPYEDASDSRYGFTNENVVFVNKANSAYKAESERGDEFLLRHIIRLVA
                     EAIAEEKHPEGKDALELQNRLIGEAIKIHNLHSRKK"
     gene            23242..23838
                     /locus_tag="VF222_06700"
     CDS             23242..23838
                     /locus_tag="VF222_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX7142149.1"
                     /translation="MAYQYFPGATAVGISFNNGVLLASEQRVSFGNFLVNKNTKKTFV
                     LTENVGAACAGMVADMNVLARQVAALSKIRKMEIRREVPTNSVAKLMSVIMFERRYFP
                     LLTQVIVGGYDDKPRIYTLDPLGSLLPDEYAAVGSGAEMALGILDAQYSSQMNEDDAK
                     NLAIKSIRAAIQRDAASGDGIDILTISNRGKNEVSLKV"
     gene            23923..>24503
                     /locus_tag="VF222_06705"
     CDS             23923..>24503
                     /locus_tag="VF222_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7142150.1"
                     /translation="MDARNIGLRNIEVADTKISSIDGENGKLIYRGYDILDLVNHSTF
                     EETAYLLLFGELPDKNQLDEFSYKLLQARKLPDSLIANLENRPKTARPMDVLQSCVPS
                     LGDFDESISSDTKDDNIRRAISLISKIPSIVAAWNNVRNGQEIVEPDQELKHSANFLY
                     MVFGRKPTFEEVKVFDICLILHAEHSFNASTFAA"
BASE COUNT         8403 a         3864 c         3888 g         8348 t
ORIGIN      
        1 ctttataagt tcaaaggctt caataagatt tgttccattt atgggatctc ctcctaaccc
       61 aagacaaaat ctttgtccat atcctgcatt agatagagta tatgatactt catacatgag
      121 agttccactt cttgagaaaa ctactgtcga acctttagtg aagggtttag caggcataat
      181 acctaccttc attatttctg gtactatgac tccgggagta tttggcccaa tcattctggc
      241 acctcttttt ttagcatatt ctaaaatttt tatggaatct attataggaa catgttccgg
      301 aactgcgact agtaacttta ttccattatc tatcgcttct tttgctgcac ttaaaaagaa
      361 tggtgcaggt acaaaaattc ctgaaatttc tatggaattg ttatttacag cttcagcaac
      421 tgtattgtaa acaggtatat cttcaaagga ttgacctccc ttgcccggtg taactcctgc
      481 cacaatgttt gttccatagt ctttcattaa ttttgtatgg gtggacccaa aactaccggt
      541 cattccttga attatgacag gtttattttt ataatcacta tcattttcct taccaattaa
      601 tatattgaat aaatcaaatt taaggctcat ttttattcac tttttctatc agtatttgac
      661 agctgcattt attgcttctt ctacagtgtt aaataaccca gctttactat ttttaagcaa
      721 ttctcgagcc ttttcagatt ctgctccagt tattcttgca aaaacgggga ctttaatagt
      781 accatcatta tatgcattaa gaatagcttg tgcaactaga tctgttctta ctattccacc
      841 aaataaattg acaagaattg cacgaatttt atctacttta cttattactt ttaatgcttg
      901 atatattgat tcagttgtag cccttccacc aaaatctaaa aatgctccag cttttcctcc
      961 agcatcacta accatatcca aagttgacat tactaagccc gccccattac caatgattgc
     1021 catatctcca tccagctcca caaatgaaaa tccattcttg tttgcctgcg attctaattc
     1081 tgacaattct tcatactttt ttatatcgtc atgtctgaat aatgcattat catctatgat
     1141 cactttagca tccaatgcaa caaatttccc accatctagc atggctaatg gattgatttc
     1201 tgccaattca gcttcttttt cataaattag ttttgataac ttttgaatta aatctataaa
     1261 agtattcttt tcagaattgt taagattcat tttatctgca acttcagatg ataattctga
     1321 tgaaatccca ttaacatcgg gaatatcaat tataatattg ttatctacat tttctatttc
     1381 tatacctcct tgggaagaag caatgattga ataacacctt ttagttctat taagaaaaat
     1441 agataaataa aactctcttt ttatatcaac catttcttca agtagaattg tcctgggctt
     1501 ttctccattt acttccttat tgattagttc ttcgaatttc tctatgagtt ctttttcatt
     1561 tttacatttt aatattgctc cagcctttcc tcttccacct actgttagtt gtgattttaa
     1621 tacaaatgga aaaccaattc tttcagaacc tcttttggct tcttcaatat ttcgtgccaa
     1681 atacgaattc gggactttga tcccataatt tttaaaaagt tcctttcctt gatattctaa
     1741 taatttcatt tttacaagga gattaatttt ctgagccttt atattcttat tttttaacaa
     1801 atatcaattt tatcgccttc ccaaatacca tttcgatact atacataaaa tagagagaag
     1861 aataagggat tacgatgata gaatttttaa gagattctta taaatatatt ttttgtatta
     1921 tgtcattttt gacgttttac tccaattttt aaatttgtat tgccactatt tttcaagatt
     1981 tctctctatt acctaataaa aactggaagc tatcagtaac caatgtattt tttatcatta
     2041 aattaataac aacaaacgat atgaaatata taaatacaag aataaatagg aaggaaatat
     2101 attttgaaac tatatacgta acttaagttt caataattga gtttctaaca ccttagatgt
     2161 gtaattgatt gattgactga ttgattgatt gattgattga ttgatcagac tcataaagaa
     2221 aaaagactgc ttcaaaataa aaactgaact gctaaaattt aataatattc cactttcaat
     2281 aacatttatg taactctatt aaaaatttca ctattattcc agctttaatt aatcagaaaa
     2341 gatgtaaaat ttaattactg tttacatatg gacagtctat atggatttag aatcaaagct
     2401 atcacttata acttctgaac cgacggaaga aattgttaca ataaatgagt taagaaatct
     2461 tttacagact atttcaagac ctaaacatta tataggctta gaaatttcag gtaaacttca
     2521 tcttgggagt cttattctac cagggtttaa gataaatgat ttaataaaag cgaatttcaa
     2581 tactacggtc ttcctggctg attggcatac ctttatcaac aataaattag agggaaattg
     2641 gactaaaata gatgaactat ctaaatatta ttctgaagct ttcagattct tttgtcctgg
     2701 cgtaaatatt attcttggaa gtgaacttta cgtggaattt gaagattatt ggaagaattt
     2761 catgcaattt tgtaaacaca ttactctatc aagaactatg agatccttga caattatggg
     2821 aagaaatgca aacgaaaatc tcgatttttc tcaatttctt tatcctgcta tgcaatcaac
     2881 agatatccac gccctcgatt tagatctggt acattccggt acagatcaaa gaaaaataca
     2941 tatgcttatg agagaagttt tcccaaaatt gaaatggaaa gttccaatca gtttgcatca
     3001 ccatttactt ccaggactct ctgaacctgt caagctagga ttaaccgagg atagttcaga
     3061 agatttaagg atttctagta aaatgagcaa aagtaaacca atgagtggaa tattcattca
     3121 tgatgatgac aaaacaattg aaaccaaaat taagaaatca ttttgtccta ttggtgtttc
     3181 agagaataat ccaattcttg aaataataag atatattata tttcaccgtt tcaacgattt
     3241 tataattgaa cgtccttcaa aatatggagg aactatatct tatggtaatt attcagattt
     3301 aaaagaagac tatgacaaac aaaaaattca tcctcaagat ataaaacaat ccacctcatc
     3361 atatctgtgt aaactaatag aacctataag aaatcatttt aaggacaatg aaccattatt
     3421 ataaacgttc aaagaaatgg aagtaattaa gtataggtgg ttactcactc acgattaaaa
     3481 tttctctttc actactattt ttcactactc tttgactaac tggtattttg atttatatcc
     3541 cctaggattg atcatcatat tattatactt gtatgtagat gagtttccta ttattatagt
     3601 agttatcatt cctatcatat cactataatt taacatgtct tgtaaatttg ttaaaactat
     3661 ctcttgacta tcacgatagg ctccttttat tattgcaaca ggagtatctg gtttcctata
     3721 ttttaaaagg atatcccttg tatcccttag ttgatgaatt ctttttttgc ttgctggatt
     3781 gtatattaca gtaacataat ctccgattgc agctgcttct actcttttta ctatcatttc
     3841 ccatgggacc aatagatcac tcatactaac tactgcaaaa tcagtcatta atggagaacc
     3901 aactaatgct ccacaggaat ttaatgaaga aacgcccggt acacattcaa catacatacc
     3961 actttctttc ttccatcctt tctctgctaa aatttcataa attaaaccaa tcattccata
     4021 tatccctgga tcacctgaag aaactagaga aacaattttt ccattctcag caaattctat
     4081 tgcctgattg gctctgtcta cttcttgtgt cattgcatat cggtaaacct cctttccttc
     4141 aattagatcc tctacaagag aaacatatgt gtcatatcct actatgattt cactctcttc
     4201 aattacctgt tttgctctat atgtcatatg atcatgatta cctggcccaa cacccacaac
     4261 atataatttt ccttttgaca acttgtatat actattaaaa acaaaattta tctttttgct
     4321 gatggtggat catttgatat ttataaattc ctgatgaaga atattaactg cttcctcgca
     4381 atttttatca tcaatgacaa atgcaagatt aagttcagaa gatccttgtg ttatcattat
     4441 tacattaata tctcttttag ctactgctcc aaaggtttta gctgctaccc ctttaattcc
     4501 tctcattcct gatccaacta ctgcaataac ggcaacattt tcactaacat caattctctt
     4561 aattaatttt cccaagaaat ttaactctag cgtcataata gctttgtcta aatcttgttt
     4621 ttttacgaca attgtaatac tggactcaga aggggtttga gaaatcatca taatattgac
     4681 atgatttttt gctaatgcat caaaaatttt tgccgcagtc ccaggagaac ctattaatcc
     4741 acctccacta acatctataa gtgctgtgtt tctaattaca gttattgatt tgacaatctt
     4801 attataagct agagtgggaa tttgggttat taaggtccca gggtgatcga tattatttgc
     4861 atttcttatt ctcactgcaa ttttagaatc catgacaggt tctaatgctc ttgggtgcat
     4921 atactttgaa ccaaacatgg ataattctaa agcctccgaa aatgaaatct cttcaaggac
     4981 tcgagcgttt ttaacaattt ttatgtcagc tgtcatcaat ccattcacat cacccaataa
     5041 ccaaacctca ttggcattta tacttgcagc aattatagtt gctgagtagt cagagcctcc
     5101 tctcccaatc gtggttgtat taccattctg atcgcaaccg ataaagccgg taacaacagg
     5161 aattatcctc tgttttatta atggctcaat tttatgatgt actctaagtt tggttgtatc
     5221 cattaatggc ttagcatcac caaagttaga gtctgttaag attcctactt cctttccatt
     5281 catgtatact gaatttatgc cagcggattt taaagcaaaa gatactatag gggttgatag
     5341 tcgttctcca aaagaaaaaa gataatctct atattttgga attatatctc ccaaaatgga
     5401 aatgcctagt aaaacatccc tgaattcatt taataaatct ttaagcaaat ataagagctc
     5461 atgtttatct ttctccttgt gtatgacttt ctcaaccaga tttatgtgga cattttccat
     5521 atccttaaga aacaattcaa gtgaatttct attaattttt ttaatggcat cacataattc
     5581 tgatatttta tccgtaattc cataaagagc agaaactaca actacaattt catagtctaa
     5641 ataataatat gattttatta tatttactac attgaggatc tttttttcgt tatccaatag
     5701 tgatcctcca aatttcataa caatacgcat atacttgatg agtattctgg ttcctagatt
     5761 aatgcttcgt taacagccag cataacccta aagagagaga atttaaagta ttattattaa
     5821 tcttggatac gtggagctag gtaaaagtgt attcttccaa gatttgccac agtaaattct
     5881 aatcttagag gcattttgct agaatattca gcaatcacag acccaccaac tgcacttatt
     5941 gcctttgtga tctttattaa ataatctata ctatatgttc caagactgtc ttcagtagat
     6001 gttaactctt ctaaaccttc attctttggt tctaaaaata tatcggcttc acctgaatca
     6061 cctctgccca aaaatcttat attctctgat ttgcaatcaa tagttatgta ttctgacagt
     6121 acttgaatat ctgataatat tttatcaaat gcagaagctg ttaatattat ttttgaatta
     6181 aaatttaatt ttggtagagg agtcgaactc gaggcacttt caattaatct cattttatat
     6241 tctttcttat atccattttg tattttgaga atcaacatac tttctttgtt tatggataat
     6301 tctattgaat cccttttttc agatcttttt attaatttag aaaattcatc gattctcacc
     6361 ccgaacttga ttaatgaatc acattcgtat ttttcaaagg acgaattagg ccaataaata
     6421 tcaattaatg ctacgtgtga aggatccatt cctctaaagg atatcccttc aaccgatgct
     6481 tcaaaagttg cctcatctac gagggtagat attgcagatg taatagcttt ccattcatct
     6541 ggagtttttg tcttagctaa aaaagtcaaa ttaatacaat gttctggcta acattagaca
     6601 attttaaatc ttcctattat aaagtcaaga aattttctag tgatgtaggt tacctaatgt
     6661 taagaatagt ttctccaagt gtggctacat ttcgtgcaac ggtagaattg ggtagtggct
     6721 tcatcagcag accgagtctg gagcatccac cacactgcca aattattatt acatttggga
     6781 caatctatac taatagtagg aagagtctgt gttgtatttt caccgtccaa tattcttaaa
     6841 gcactttgtt cttgtgttgt tatgttacca acattaactg taacagtcga tttttgttga
     6901 gaactagtaa atccgcattt aggacaagca acttgatttt cttcaaactt caaacgcatt
     6961 cttgtttcac aatgtgggca gaatttcaat ttaaagcacc ttttgttcag aagattcaat
     7021 caaatgtgaa atatctttta aaaactcatc agcggatttg attgacttgt ctaaaatatc
     7081 cattggattt tctttgcttt ttattttcat gagatactct ttgatcaagg gatgcttcat
     7141 cacgataccc gcaaactcta tatctttctc ctttaaaata tgatgttgga ttatgtacat
     7201 aatagagata tcttcatctt tcaactttag ttctatctca ttattagaaa aggttgtaat
     7261 ttctgcttgc attaaatagt caagtacata tttaggatat aaatcatacc acatggtaat
     7321 taatcagtga ctgaaaaaat aaattcatta gataatattg ttttaaccaa tataaccagt
     7381 aaaactaatt caaaggaaca attatctttt aatttaagtt taaaaataaa tggtaaaatt
     7441 agagatttat ttactcaatc attatgggaa aaagcatata ataaatttga taacaccttc
     7501 aggataacaa taaatgtgga cttgaaatac aaaagcaaag tcatatactc tgtaaaattt
     7561 gtaagaaaag cagttttttt ttggactagg aatccaaaaa ttcatcatag aatatggatt
     7621 tttatcgtta aagatgacag ccctttctat cctgaatcag aagatgaagc caagtctctc
     7681 ttatttgata tagataatat cgttgaaatt aaattaccag caattatcga tgaagtatct
     7741 actctttatg ctaatgtagc tgtttcttgg ggaaaacata actttactga tccaacaaaa
     7801 ctagtaggga agtctaacga ggaagaaata cacgtagtca attcgtaagc tgatatattc
     7861 tttttagttt tatattaata atagaaattt ttaaaaatgt taatctacaa ttaatatagt
     7921 atggcaaaat ttgacgggat aaaaggacaa gagctattag atattgagat acaaggaaat
     7981 gaactaactt ttatatttaa agataatcgc tatctatttg ttaaaaatga gaaagacaaa
     8041 atgatttgtg attcattacc agaataactt ttcttcattc tctactttta tattgagatt
     8101 cactaattca tatttgatga gagagattta tctataattt ttgtttaaaa aatacacttt
     8161 acatatgtaa tcttgtaaga aaattagtct cagttttttt tacaatcata gatttatagt
     8221 tagtcagtcc atgatagaaa tatggccatt atttaggagt gaactttaat gtcctttata
     8281 caataaaaat gcattattat tattgagaga tattatttag aatactaatt ccttgacaat
     8341 atggttacat gattctctga ttctacaaac tatatctaag taaaacataa tgaatttaaa
     8401 tattaaaaaa gactcaatta tgttgaatat aagaactgct gaactaaaaa aagatgacta
     8461 tattggaaaa tatatttcta ttaaagggtg gatacatcga atccgaaagc aaaaagaaaa
     8521 tacattttta ttaattcgag atgatagagg aggcatcatc cagtgtatta ttgaatcatc
     8581 aaaaacaaat aatttaacta tcgagtcttc tgttgaaatt tttggtattc ttcaaaaaga
     8641 ttcccgcgca ccagaaggag gttatgaatt aaaagcggat agacttaata tttttagcat
     8701 ttctaaaaac gattatccaa taggagaata tcaaagcctt gatcttcttt tagaaaaacg
     8761 acatcttact ataagaactc gacgcatgac tatggtttct aaaattcgtt cttctattct
     8821 ctatttttca agaaaatggt ttaccgataa tgattggatt gaagtaacgg ctccagtaat
     8881 agtaaaggga tcagtcgaag gaggttctac attatttaaa ataaaatatt ttgacgaaga
     8941 tgcctatctt tcacaaagtg cacaattata tttagaagct atgatctttt ctcttggtcc
     9001 agtatggtct atgactcctt cgttcagagc tgagaaatcg agaacagtta gacacttaac
     9061 tgaatttctt catttagaag cagaggcacc atggatatct ttagaggatc ttataaaatt
     9121 gcaagaagaa ttaatttcat ttgtcatcca aaatattttg aaaaacaata aagaggaatt
     9181 tgattttttg aaaagagatg tatcagattt ggagaaaatt tcacctccat ttgatagaat
     9241 tacttatgaa aaagcaatag ataaactacg aaaagaagaa tttaagataa tagagaaagg
     9301 atcgtctaga aaaattgaat atggtgatga tcttagtatc gaatcagaaa gaattctaac
     9361 aatcaactct tccaaaccat tatttgttat tggatatcca ttgcaaataa aaccatttta
     9421 tgttaaagaa gaaatctcaa aacaaggatt aggaattgca gcggatttac ttgcacccaa
     9481 tggttttggt gaagtaacat ctggcggttt aagagaagat aatctccaaa aattggaaaa
     9541 tagaatggtt ttggaaggtt tacaaaaaag taattatgat tggtatctgg atttacgcag
     9601 atatgggtca gtaccccatg gaggctttgg cctgggaata gaaagactaa tgagatggat
     9661 tctcgatatt actgatataa aggatacttt gccttttcca agaactatta gacggatata
     9721 tccttaaact ttttactatt ttgttaaata aacaattacc tttgactata tttatttaaa
     9781 attaatttct tagatttatt caagtttaaa tctttggaaa gaaatactat aataaaatgc
     9841 ataataacct tcccgaaaaa accgagtctt tattctttga attggcagga gatttgaggc
     9901 tttctatgtt attaaagctc tctaaacata agtacagatt atctcagttg tcaactgaat
     9961 tggatgcaac tatgcaagaa gcacatagaa acataatcag attagttgag agtggattgg
    10021 tttttaaaga tggagaaggt gatttaggat taacttctta tggtaaatcc attgtttttt
    10081 taattccagg atatgaattt cttctacaaa ataaacagta tttcattgaa cattcgatag
    10141 aagaattgcc attaaaattc attcaaagaa ttggttcttt acaaaatagt gaaatagtat
    10201 taggagtaat ggcaaatcta cagcgctgga agactcttta ttggaactca caagtctata
    10261 ttaaagaaat tatgtcacag gtgccgttag atttaattga aacagtaagt aataagattc
    10321 agaccggtat taagttttct tacatttttt caaaagacac aattattcct aaaggaagag
    10381 gagaattatt acagaaaata ggttgggtta accaaatttc taaaggtatg gtagaaagaa
    10441 agatgattaa taaggttact ataatgacaa tattcaatga gcaagaagct tgtttgctat
    10501 ttccaaatct gaaaggcgaa ccagatttga atattatgtt ctatagtaaa gatcgaaatt
    10561 ttcatgaatg gtgtaaggat ttttttgatt accagtggga aatggctggc ccatttgacg
    10621 aaaaaaaatt aaatgaaacc taattacccc tttactattt tttcagcatg tttttatata
    10681 tcattttatt ataaaatgtt atgcctggtg cttttgtact tttaaatacc gaattgggaa
    10741 gtgaagattc catcgtaaaa gaactgagga atttagaagg agttaaagaa gtatttcaag
    10801 tctatggtgt atatgatatt attgcaaaag tagaatctga tacaatggat aaggttaaag
    10861 aaattattac atggaagttg agaaaattac cgggagttaa atctacactc acaatgattg
    10921 taatggagta aatttttttt ttatcagtat gatgtataga tctaatgaat tgtagaatta
    10981 atataattta aaaagaactt tgtatgtgaa ctatattatg gggttttcta atattttaaa
    11041 aagaaaaaaa ggtaaagaag caaatgagat aaaatgtaaa aattgcaata ttgtttttca
    11101 agataaagag catctttcac gacataataa aaaagctcat ggaaagaatg atgatttttt
    11161 accaagtagt aatccttttc aagtctagta cgctactatt agatccagca tagtaaatgt
    11221 ttcctattca tgatgatact cctcgtcttc atggacgacc gtatgtaaat tactctctta
    11281 tagctttaaa tgttattata tttatatggg aagtaataag tacaaattat ttcagtaatg
    11341 agttaaaagt caatgaaatc ttttttactt atggtaccgt tccagaccgt gtttttcagg
    11401 gggattattt tactttaatt acgtcaatgt ttcttcatgg tggcgttgtt catataatag
    11461 gaaatatggt atttttgtat atatttggag acaatattga ggatagattt ggacacttta
    11521 aatatcttat tttgtatttg atgtggggta tagcagcagg agtaatccat agtttctatg
    11581 cagtttcagt cggcggtggt aatattcctg cagttggtgc ttccggagct atatctggag
    11641 ttctaggagc ttatttggtg ctttttccta gggcgaaaat atttacagtt ataactgctt
    11701 tttttattac aacagttaga attcctgctc ttgcttacat acctgtatgg tttatccttc
    11761 aggtagtatt tagtatattt aaccccgaag ggggagtggc atatttagct catattggag
    11821 gttttgtagt aggagcaggt gttagtttcc tagcaaagtc gtttttccct tcggtatttc
    11881 aaggtcacat tggagtgagt agtatttatt caaaaaaatc ttccaagccc tattctagac
    11941 catataatca ggataatact agacattcga tctctcaacc agatattatt gaggggaata
    12001 attattacga aattttagta gaactgaaag gtttgagaaa tatccaaaat ttacgagcta
    12061 tatatgaaaa aagcacacat agtttacaaa ttttttcgga gggagctcct aagcctattg
    12121 tatctattcc acttagagaa gatatgcata tatctcaaat tcaggatgta tcctatatca
    12181 atggaatcct tagaatcaga atgttaaaag ttaattaggg ctttgtctta gttatacact
    12241 agatgtgtta aaatgttctg cattggaaca aagatttata atatattttt tcatctgtaa
    12301 tggtaatgtc tgagcaaccc caggcaaggg ggaatgataa aatttcacgt agagattttt
    12361 taaaattatt ggcagcagct ggtactgtgt tgacatttac accttttgtg gactggggga
    12421 aatttttgcc taatccaagt ggttcaacaa ctgaaaaatc taaagttcag ctacctgatg
    12481 gttctcaagc taatgtaaaa acctttaagg ttaatcattc tgaagctata atttatccta
    12541 tatctgagga tgctgctctc aataaagagg cattcaggac atggcaattt atacgacttc
    12601 cacctgaact ggggggagag aagaacgatg cttcagcctt tcgggtcttt agcatggttt
    12661 gcttacatct ttggtgtcta tggaagtatt ggcccggtga gggacgaaaa aggggtgaat
    12721 gtccatgtca tggcagcctg tatgacccac taacaggagc ggcctttgca ggccctgccg
    12781 ctcttcaatc accaccttca aacgttctac caaaactcga tttggaagca gataaagatg
    12841 ggtatttatg gatattgcct cctaattgga atgttaatgg aaatgggata gtgggatatg
    12901 gccgcttcat tgcatagaga aaacagtcta gttagactat tccggtggat ctacaccggc
    12961 gttgatagaa caattttcat gggcttaaaa ttcacatttc ctactcggtt tgttagtcct
    13021 ttaggctttt taggaatgct tacttttgtc ctcttcataa ttctcggtat atcgggtgct
    13081 cttctaatgt tatggtatga accaattctt gacagagcat gggatagcgt tgaaaatata
    13141 aatgatacta taccatacgg tttccatctc agaaacattc actatcatgc atctaatgct
    13201 atggttatga tggcaattct tcatatgtat tatcagtatt ttagtggtag gtataaaatt
    13261 agaaatgaaa tactttgggt tactggaatc cttatagggg tattaaccat tttagaagcc
    13321 tttaccggat acgacattat ttttagtgaa agagcagaac ttgctatttc tatagctgcg
    13381 tcccttacta attctatacc tattatcggg cctgatatga gagatgcatt ctttgggtcg
    13441 gggtttcatg actttgtatt acgcttttat gcattccatg tatttttact tcccattgca
    13501 ttgataggtt taatgactgt acatttccca cgttttcttg tttttgatgt tccaatggtt
    13561 atggctatca caggagcaat aatgattact ggaggagttt tcccggtgga tatgggttta
    13621 aagtttgatc ccaatgtacc acctggaata actgtacctg aatggtatct cacaggattg
    13681 tatgcttttc ttagatctca gtacgataaa tttgtaactg gtgttgcttg gccggggctt
    13741 ttcatattta cattacttat catacctttc atagataaat ataaaaaatt ttcttggaag
    13801 gatagaccac ttatatctgc aataggaatt acaagtattg ctcaaattgt agtgactacg
    13861 tactggggtt tctacatcga tcctgatagg acaaaatctc tattagacag attggtaatc
    13921 gatccaattt ttctctatag tgtaatgatc ctcttagttc cattatcatt tggatttaca
    13981 tatcttatga taaaattggc taaaaatgca gaagctaatg caaaacgtca gccccgaaag
    14041 aaggagagtg taataagtct ccctgaaaaa tggatattgt ttgtatttat agcattgata
    14101 ggatttcaaa tctatttgaa tattgcagca tattatgctc tacttaatgg tatgaaaaac
    14161 atatcgttgt tcattattgg tattatgatg ctagtttttg caggaatgtt ccatatttat
    14221 agacatggac gatccaatgc aaataatcca cctcctccct ctataccaca gactgatagg
    14281 attaagtctt tacaatataa aaaaagagca ttagtagaat ctgataatga taaaccggga
    14341 ttagaatcat ctgaaaattc cattggtcaa ataaagagca atggaatttt gaataaggtt
    14401 ttcaaagtcg ccagaaaaaa taaaaattca ttggaaaaaa ggtggaagta gaggttagta
    14461 acaatcctaa aaaaaataaa aaatttaaat taacgaaact atctactatg ttatattagt
    14521 atggaaagat cacagcagtt tagtagtatt ggcgggttaa ctataatatt ttttatcata
    14581 gcaataacta ttagtttggc attctatcag ttcgtctttc ttaaagaatt aaatctcaag
    14641 ccttccgttc cagaagaagt agcaaatcct ccacagccat tgaaattaac catagctgaa
    14701 ggtgcgagtc tgccaactaa tcctcaattt tatgttccct ctattgcccg agggtcttta
    14761 ggtattgata ataaattaat ttgggaaaat ctagatacta ctcctcatac cgtaactacg
    14821 gataatgatt atcatgatcc tttgtctgga aagtttgact caatggagac aataggactt
    14881 gttccaccag gagggacctt tgaatttgta tttacagagg atggagaata tccatatcat
    14941 tgtgaaccac atccttggat gactggtact gtcacaatag ttaagaattt ttcatagaat
    15001 cttttctttc atactttttt tatttgaaga aacaaaatat catttgccac ttctttatgt
    15061 tcttggatga tagaagttct aaatttaact ttagaaaaaa agttttttgg tacatgttgt
    15121 gtaatggcaa taaaatcaat aatccttttt tcttccaact cttttttgta gatagaaaga
    15181 cgtgcatatg gatgattaat tttttttccg tttaattcga tataattaca aatatcattt
    15241 gaattttcaa tttgcgaatt tattacgata gtatcgaatc ttccattata attcacttca
    15301 atactgccat gaatttcttg attaggatga atttcatttt cttcgatagt aagagataat
    15361 tctttttttt tttgcatagt tataaagaat tctttctgat aattgtatat atttattcag
    15421 aataatctaa tttaaaaatt aaacatatag aataaaatat ttgaaataaa gtaataatct
    15481 ttaaatttgc taaagctttt tcctgttgtg aagggctcgt agctcagcat ggacagagcg
    15541 cctgccttct attcagtctg agaagaaagc cggaagtcga gggatcgaag ccctccgggc
    15601 ccgctatgat gttttcctta tttagtagaa taaaaagaca cctaagacaa cgaatagaaa
    15661 aaaagcaaaa atcagcagca tcaacattgg agtgagtaat catttcttaa tatacaccta
    15721 taatatcacg aactacattt gtttctatta tgttgcgttt tacttcattt tgtaaaattg
    15781 accctctata gagaagatga cgaaaaaaac tataagttac ctggctgaaa atgcaagaca
    15841 tatttaagga ccttttttta ttagaaagat tatatcaaaa acaaacgtat atagaacaat
    15901 agacagaaat aaacaaaatt gtataatcaa atatacctat ttaaccttaa taaaaaatta
    15961 ttgaaaatca aatatagagt tatatgaaga aatcaactat tttataatat gtttttttat
    16021 aaaaaaatta tcaagtctca tctgacgatt tagtatatat tatttatttc ataaaccagg
    16081 aaaaaataaa atgggttatt attagcactc taccatgctt tacatatttt ttcattatag
    16141 ttataaataa actacatttt agatggattt ttggactaag tggggttaat ttgcttatac
    16201 tattttcagt atcctaaata cagagtgaat tcaaaagaaa tagacatcag gaaaacctat
    16261 ccaaaatagt tttattctat tataaatatt tttaaataat tatactggtt tattagttaa
    16321 gaattcacca attaatttaa tggtaaatcc aatcgcaccg actactccca tccctagtaa
    16381 tacaaatctc atatgggttg tataatcatc tttagatgtt tttttcgcta ataaaagagt
    16441 ttgatacatg tcaagaagcc tccttcttaa ataagaaatt atatttagta ttttcataat
    16501 attacctttt actgcaagac agagtatttg aatttaactg tataaattaa cttttaatat
    16561 ttaaaaattt taagtcaaat taatatactt gctcagaata atctggatct ttttcagttc
    16621 tttgcatttc aacaaatgtt tcagtatttt gaattccttc gattttacca atcctctcaa
    16681 ttacgatatt atgcaaatct tctaacgtac tcgcattgac atttacaatc atatcaaatc
    16741 tacctgtaac ctctgataag gatctgactt ccgcaatagt gagtaattct ttgtgaatgt
    16801 gatcctttct ttttggatca cgattgatcc ctactgttgc ttttatgttg agaccaagca
    16861 tgttttcatt gaccatgata gtaaattttt taattagatt tcttttggaa agacgtttta
    16921 ttctactgta taagaccgaa gaatttatat tcaaattttt acttaactgc ggaaccgaaa
    16981 tactagcatc acgagtgagt tcagtcagta ttttcatatc gagttcatca aacctttgca
    17041 agttatatcc ggaatacgtt ataaatctag attaataaat gtaattatat attaaagatt
    17101 taatttaaat tgttattatt ttatattaat tttttcaatc tcattccaat atgttataat
    17161 attttatatt aattttacaa tagataataa tgttatagaa aataatatat aattttagct
    17221 tgtaacatcg aataatgttt ggactaggta agatcaagac tggagattat gtctttgccg
    17281 tagaaccgga aagtgaatcc aaaagaatta ttttaggaag agttacatcc gtcaataata
    17341 attcgctctt aattttggga tcttatataa agcctttagg actcctctcg aagagacaaa
    17401 aaggattact aggaaaaaga tctcaagagg ttttaaatga tcccgatccc aataattgta
    17461 tttttattct aatcgataga attgaaacag gtaattttta tggtaatata gatcaaagta
    17521 taaataaagt aagctggata aatgaaaaaa gattttttgt attggaaggt tggattaaag
    17581 agaatatggc agatctgttt gcagatgtta tttattctca atctgaagac gaaaaacatc
    17641 atttccgtgc tattttaata gagaaaatga attctcttta tgatagagaa ttaaaagaac
    17701 atgtctatgc tgttgctaga ggtgcaaaga ttttgtaaca agttgaaagt tttatattat
    17761 ggttttataa taatccgttt ttgataacta atggaatacg tgtatgcggc attattatta
    17821 cacaaattga aacaagatat tactgaagat aatgttacta atgtaattaa agcaactggt
    17881 tctgcccctg atactgtcag aataaaggct cttgtagctg cattgggcga tgtggatatt
    17941 gaagcggcat taaaggctgc cccaatgact atggcaacag cagcggcagc acctccagcg
    18001 acaggagcag cggcaactga aacaactaca aaggaagagc ctgaggaaga gaaaagcgaa
    18061 aagaaggaag aagaagcact cgaaggttta tcatcattat ttggataaac ttttttatta
    18121 tttatttttg attttcattc cttgctattt tattacagtt tacacagttt gccatcaaaa
    18181 tttatttaaa atcccaaatg tttccgaata aatgtaagtg cggacgatta ctcaagtcat
    18241 ataaaacatg caggccactc gtctgcgatg aaattttttt atgcgagaag tgtcactttg
    18301 ataccaatga ttgtatatgt aatttggtaa aataaaatat ttacattatt atactttatt
    18361 ttgtcatagc gataaatatt ctagtgcatg ttttgtggaa atagaaattg gattaatatg
    18421 catattatat acatagataa tagaacaaaa ccaaatctta atcctatttt ttttctaaac
    18481 atcataggta gaacagctac tattgtgaca attgtaacta gaataatttg ataatacaaa
    18541 atttctgtca attcaattac tttattaaat ccaaatgaat acattgccgc aaagactgct
    18601 actgccaata ataatgtatt attaagtatt ttagaaccca acacatttgc aatagctatg
    18661 gacgctcctg cggccccttt cctagccaat atcatcattg aaatcttttc gggcatttct
    18721 ccagcaattg gactaattat aaccgccagc actacagcgg atattccaaa ttctacagaa
    18781 accccctcca aagaatgaat aaaaggttct gcaccaaaaa gtatccctat agcgcctaac
    18841 aaaaaagttc caattgcttt gatgaatttc ttattatcat tattatcatt tgaaatattt
    18901 tgctcaacaa ttaatttctt attattgtta ttattgttat tgctgatatt cagactgaca
    18961 tttttatcag agactttatc tttattttct actctcattt catgtagtga taatattata
    19021 taccctgcaa agagaccgac gaaaatgaac ccatcaagat aattaaatcc atctaaaaat
    19081 agcacaaagc ttattacggc agctactatc aaaaaaatca tatcattttt aaaagtacta
    19141 acatcaacat gtttaagcgg cgccttcgat aatcttgttg ttgctataat caacataatt
    19201 gctaaaccta atgtcatcat caacaagtta ctccctagtc cagaaccaat agcaatacca
    19261 aaagacccct gcgctgcagc ataaatcact atagcgattt ctggcaaagt agttgctata
    19321 cttaataaga ctcggcctac gaatttagca ccaaattttt cagaaaggtg ttccgcccca
    19381 taggatagta tccaacttgc aagagtcagt tctcccaacc aaaaaagcat agtaaagata
    19441 ttattttctg ccattttggg gataagaatt agtataagtt attatctgat tatagccatc
    19501 tttttgcaaa atctttatca aatgaggttt gatagaaaat aaattttgtc ccattcatcc
    19561 atcaactttg gataaagggg aatttagtga tcacattata tggttgaatg tctaagaatc
    19621 cttcttccat aaccgtaatg aattgagtag gatgaggtgc agtcaatatt acgaggaaga
    19681 ctggaactgc ggcacaagtc accatagtca gtaagattaa ccaaagagca gtcatttttt
    19741 cggccatata caaaaatgat tcgacgtgtg atatataaat tttataaaat tttttgtttt
    19801 caaaaataaa ataaaatatt attacactac aataacttta ttttttattc taaacgattt
    19861 taagtagctg attattttat ctttatacat ttaccatgac aagtgatgtt gaacttaaag
    19921 atcttatcga gcatgctaga agagattata aaaagcgtaa cttcgatcaa tctctagatc
    19981 ttactattgt cttaaaagat attgatgtaa aaaaaggatt tagtttcaat gaagttgtac
    20041 atttaccaaa taaaccaagc cgtcaagcca cggtatgtgt agtagcatca ggtgaaatgg
    20101 gatcaagagc aaagagagca gaagcagata ggataataga gcttgatgaa cttgatagac
    20161 ttggcactaa taaaaaagaa gctaaaaagt tagcaaaggc ttatgatttc tttttatctg
    20221 atacatcgtt aatggctaca gttggtcgtt ccctaggtca agttctgggg cctaaaggta
    20281 aaatgccaac acctatacca tatggtgcac caattgaaag tatattgaat aggttaaggg
    20341 gttcagtaag aattagaact agaaatcaac ttaatatctc caccaaaata ggagatgaaa
    20401 aaatggatga taaacaactt attcaaaatg caaatactat attgtctctt ttggaaaaaa
    20461 aattacctca aggggataag aacattcgta atacttcagt caaatttaca atgggtaatt
    20521 gtataagttt agcatcatta tctaacaaaa ctaagggtgg ttaaaacatg agttcagatc
    20581 aaataatcaa aaaatatcct caaaagaagc aacatatgta tcaagaatta caggagcttc
    20641 caacaaaata taatgttatt gccttatcaa aaatgaacaa agtcagggct acacagttaa
    20701 tgcctttaag gaaaaaattt cacggtcaaa ttaatattaa aataatcaaa aataaagttg
    20761 ctcaaagagc ttttgaaaaa gtaagtaaaa tttcaggcat tgaagattta agcaaagaat
    20821 tagaaggtca atgtgcacta atgtttacta atataagccc tttcaagctt aatttgattt
    20881 tcagtgaaaa taaagttttt ttacctgcaa aagggggaga tattgcacct aaggatatta
    20941 tagttccagc aggtaatact ggtattcctc caggacctgt cctctctgaa tttaaagaat
    21001 cgaaagtaca aactaaaatc gatcaaggca gtattgctgt aacaaaagat acaatagtag
    21061 ttaaatcagg agaggtcata tcacaaaaac ttgcttctct cttatctaaa ttagacataa
    21121 aaccaattga agcaggagta ctagtcaact ttgcaatttc tgaaggtctt caatttagag
    21181 aaaaagattt acagcttgac gttaaagaat atgtaaaaca attacaaata tctttttcgt
    21241 ctgctttgaa tctggcaata gaaatagtct attttgcacc tgagacgata tcttctcttc
    21301 tatctctagc atatcaaaaa tcacttaacg tagcatctaa agctggatat gtttctaaag
    21361 atacagcaga aataatacta aacaacgcac aaacaaatgc tcatggttta gtcgacgcct
    21421 taaaagtaaa gggctatgtc cctcaataaa tttttctact tttatcaatt tttaaaatga
    21481 tttactttca ttgtatatat acaaacacaa taaataaagg caagaaagtc attttactat
    21541 gatataatgt caatacaatt aaaagttcat ccatcctttt ttaaggaatt tgccacaaag
    21601 acatgggttt ctccatatga aatagttaaa gaattggtag aaaatgcttt tgatgaagat
    21661 gctacacagg tgttaattac cattctcact agtggatcta taataataga agatgatgca
    21721 ggtatggacg tagaaggaat tgaaaaattc ttattattag gatcgcctca taaaaagata
    21781 gaatcaattt ctccaaagtt aaagagaagt cgaacaggac gatatggaac aggaagactc
    21841 tcttttctta cttcatttga gagcatgaaa atcaaaacca aaaaaaataa ttataacaaa
    21901 tctcttgtta tagatgaaaa ctctctaaat caactatttt cgggtaattc tttattaaat
    21961 gagctaaatg aaaaacctct ttctagaaat ggaacagaat taaatctaat taatgcaaag
    22021 atgaatattg accctttgaa attatataag gaaatacgta aattagcgat tcttagacac
    22081 cccatgtttc aagtatttat taaacaatcc gatagattca ctgaatggga tctagagaag
    22141 tctgatatag taaaggccca agagatacaa ggctaccgca taccggtaaa tcttgataat
    22201 ggcaaaatta ttggtgaaat tgtagttgca agacgtccat taatagaaga ggagaaagga
    22261 ataggtgtga tgattggaaa tcacattgtt gttagaagta attttggttt tgatgcgaaa
    22321 ttgaatcgaa ttacgggatt actaagatgt gatgctctaa cttctagatt tgcagataaa
    22381 tctgcattaa tagaggatga tgcttattat aattttaaca cgtacgtcaa acaatttatt
    22441 gttgatgagg taatacccaa attgactgaa tatgaagatg tattgataac taaagaagag
    22501 acacgaatat atcgtgaaat agacaaagtt cttggccaag cagtaattga gaacttggag
    22561 tctgtagaat tagaagaaga ggaatatgaa acagttgaag agaaagtaac cgttcctaac
    22621 gcagatagat taactataaa ctcatcaatt acaaaggtca agattcaaga taataaaatt
    22681 ccaacagacg aggattacga aaataatctc tctgttcaaa gctccaaaat tagagaacaa
    22741 gctcacacta gtatactaga ccatcaaact atccatagtg aaattgagcc tcactatttt
    22801 aatcctaatg aaaggacaca aactattcct aatttgcaaa catctaataa tttagataaa
    22861 atggagataa aaaaaataaa aactcttaaa cgaaccttcg ccctaaaaaa gattggatat
    22921 aaagtaatcc catatgagga cgcaagcgat tctcgatatg ggtttactaa tgaaaacgta
    22981 gtttttgtaa acaaagctaa ttctgcatac aaagctgaat cagaaagggg tgatgaattt
    23041 ctcctgagac atatcattag attagtagcc gaagctatag ctgaggagaa acatccagaa
    23101 ggaaaagatg ctttagaatt acaaaatcgt ctcattgggg aagcaattaa gattcataat
    23161 cttcatagta gaaaaaaata agtgtactgc gttaaattta atagaaaaga ctaaatctgg
    23221 ctcttatttc atataccaca aatggcgtac caatactttc ctggcgcaac agccgtagga
    23281 atctcattta acaatggtgt tttattggca tctgagcaac gggtttcttt tggaaacttc
    23341 cttgtgaata agaacactaa aaaaactttt gtactgactg agaatgtagg agcagcttgt
    23401 gcaggtatgg ttgccgatat gaatgtcctc gctcgacagg ttgctgcatt atctaaaatc
    23461 cgtaaaatgg aaataagacg tgaagttccc actaattcag tagcaaaact aatgtcggta
    23521 attatgtttg aaagacgata ctttcctttg ttaactcaag ttatagttgg aggttatgat
    23581 gataaacctc gtatatatac tcttgatcca cttggatccc tacttcctga tgaatatgca
    23641 gcagtaggaa gtggtgccga aatggcttta ggtatattgg atgcacaata tagttcccaa
    23701 atgaatgaag atgacgctaa gaaccttgct attaaatcta ttagagctgc tattcaaaga
    23761 gacgcagcta gtggtgatgg gatcgatatt cttactatca gtaatagagg aaagaatgaa
    23821 gtatctctaa aggtctaatt acttaaatcc gatggctaat caaccattgc tatcttcagc
    23881 attcttttaa tatacaaatc tcagaggtaa tgatgaatta atttggatgc aagaaatata
    23941 ggcttacgga atattgaagt agcagataca aagatttcat ctatcgatgg tgaaaacggt
    24001 aaattaattt atcgaggtta tgatattctt gatttagtaa atcattctac atttgaagaa
    24061 acagcatatc ttttgttgtt tggagaactt cctgataaaa accaattaga tgaattttca
    24121 tataaattac tacaagcaag aaaattaccg gattcgttaa ttgcaaattt agaaaataga
    24181 ccaaaaacag cgcgtcctat ggatgttcta caatcatgtg ttccttctct tggagatttt
    24241 gatgaaagta tttcctctga taccaaggat gataatataa gaagggcaat atctcttata
    24301 tctaaaattc cttcaatagt tgctgcatgg aataatgtaa gaaatgggca ggagattgtt
    24361 gaaccagacc aggaacttaa acattctgca aactttcttt atatggtatt tggacgcaag
    24421 cctacttttg aagaagtaaa agtttttgat atttgtttaa tattacacgc cgaacatagc
    24481 tttaatgcgt cgacttttgc agc
//
LOCUS       DASVEV010000146         9465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1219292, whole genome shotgun sequence.
ACCESSION   DASVEV010000146 DASVEV010000000
VERSION     DASVEV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219292"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1331)
                     /locus_tag="VF222_06710"
     CDS             complement(291..1331)
                     /locus_tag="VF222_06710"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7142151.1"
                     /translation="MNHLSSYADFPTEINKIENCKNEKLIDKAISNSNGKNINNNVNV
                     TSLNPLEKFHHIKKFDSNGYLLDSWGNKGTGKGEFLHPHGIASDSNSNVYVSDAVLCN
                     IQKFDSNGNYLLKFGKKGSGPAEFIQPESIAIDKNDNVFVVDYATHHIQKFDSDGNFI
                     TMWGSKGNGTNQFMKPWGIGIDSKGDVYVGDQENPVIQKFDNDGNFIKKWGSYGSKTG
                     QFVHIHDVTVDSKDNVYVTDGRNNSRVEKFDSNGNFITMWGSKGSKEGQFIEDHGIAV
                     DKSEYVYVVDTRNVRIQKFDSNGNFITMWGSLGCKDDQFLIPHDITIDPKGNIFVSDS
                     GNVHFRERYVCN"
     gene            1521..3173
                     /locus_tag="VF222_06715"
     CDS             1521..3173
                     /locus_tag="VF222_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7142152.1"
                     /translation="MVKIANDVKFQKIRLNDRSNYNSRYRPITFLAILGLMILFGIIV
                     YQNSEVLAIEEETSSNNNYSFSEIFKKVNQSVVQVSTTTDPNPNQLGVKLGSGFIYDQ
                     DGHIITNYHVVNGGNEFLVTFPDGSIYSCELVGFDPYSDLAVIKVIVPQEKLIPLTIG
                     NSKNLVIGQHVAAVGNPFGLSGSMTEGIISGLGRQLPSLPTESLPPLFGGNEITLRSS
                     SFSIPDIIQTDAAINPGNSGGPLLNIKGEVIGINSAIFSNTGEYAGVGFAIPSNTIQK
                     IVPSLIEIGSYDHPWLGIIGVDITPTIAEAIDLNLTDSKGFLITDVNANSPADKAGIQ
                     GGDRIANIDANREIDLGGDILIGIDGNPVRKIDDLLTYLEREKRVGEKVTVDLIRNGE
                     TLEKTLTLEKRPNSEDIFQIGNQQLSLGVTGIEVNPLLAEALNLTDSKGFLVTSVRAN
                     GPADKAGIQGGYKVEIINNIPYTVGGDIIIGIDNKTINTVQDIKNYINSKKEGDVVNL
                     KVIQDGEIKNIDAKLEVLPITSSDAKPFNKFNPFGEDREEDS"
     gene            complement(3332..3469)
                     /locus_tag="VF222_06720"
     CDS             complement(3332..3469)
                     /locus_tag="VF222_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142153.1"
                     /translation="MSKFKFVGRISMMGNNKVIWIPKEFHDQIIDLEGKQIRITIDDK
                     I"
     gene            complement(3836..4084)
                     /locus_tag="VF222_06725"
     CDS             complement(3836..4084)
                     /locus_tag="VF222_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142154.1"
                     /translation="MNAIVMTSILAVALIAGIPIIYENVLAQQPGNATQAQQPGNATQ
                     AQQGEEIIITEGEITPADKDKDDGDKDKDKDDGDKDKE"
     gene            complement(4279..4737)
                     /locus_tag="VF222_06730"
     CDS             complement(4279..4737)
                     /locus_tag="VF222_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142155.1"
                     /translation="MAKVQHYRGLDTNLDRLYDNIKQELQDQKDLEIVSEYKGIMNSV
                     PLRSIVAVNKSPKVWAGSLREIHVSITGDPNDYAVEVASGAWFGSLVWTGAVGLVIGG
                     PIGLAAGATVGGIMAYEFEKHMWNKILDVVKKESEKQPTLDSIDHYYHSS"
     gene            complement(4975..6060)
                     /locus_tag="VF222_06735"
     CDS             complement(4975..6060)
                     /locus_tag="VF222_06735"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX7142156.1"
                     /translation="MPINKINISENQSSLPLNEIKNLVGVGEISSSNNTASTLPNQTI
                     YSNNVNLIGKNASQYVFSKNKSEIHSPENNSHTLEFEKSKIALVKPTFTDAAYHNAFY
                     VFYRMYENVPANTNVTTNLNLLTSKLSSNQEGSISNVYAMLDLIKNLKWITNNSNITI
                     LSDQEVDSKLIFNKNGSNLYDIIILGHQEYVTQDEYDNLKEFVSNGGTMIFLDGNALY
                     VQVKYDKSTNQITLVKGHAWAFNGKSAWKSVEERWKNETSQWVGSNYLCATCHISYLN
                     NPFGYFHHEENYITNPTDIILLKYPFIDPGDNSYKNIVVATYEHNYNKGKVIALGLYS
                     DDIINKGVFDRFFDSLLVKYKLYNSQT"
     gene            complement(6925..7224)
                     /locus_tag="VF222_06740"
     CDS             complement(6925..7224)
                     /locus_tag="VF222_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142157.1"
                     /translation="MNLLSRISFGKTITIVTAILVLASAGFQFYHGTQAYAQETNSSI
                     EFPKESITKILNFVDKANAGLAEGDLELVSQNLGEIETEIRDQLSEINVPLNGTS"
     gene            complement(7731..8672)
                     /locus_tag="VF222_06745"
     CDS             complement(7731..8672)
                     /locus_tag="VF222_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7142158.1"
                     /translation="MNNILSQVIFGVGIVLAISALSAIFINSVALDNSNKQSSATSQT
                     TIQPVSPNENGTTPTQSSSKTNNVITSINIAKGSAAEQVKQYFDPNPAEISDNSKITW
                     INNDITIHTATASDGSFDTGLIQAGSSESEIVKGEGNVPYGCTIHPWMRASLNIVGSN
                     TKNNTESNIINANSEVLKTNASTTDQSQSKSISIPTVNEHSKETSQTEKPFFFLFNTF
                     LPAFNVTEYPHDQFSLKTLEVNQNDNLTIYFYNMEAPMGDRHSFIINAPYNINLDLGQ
                     GKNGSTSFTANEPGIFRYYCQYHQPTMNGQLVVLPNT"
     gene            9046..>9465
                     /locus_tag="VF222_06750"
     CDS             9046..>9465
                     /locus_tag="VF222_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142159.1"
                     /translation="MSGIELAAELGYGVPWLKLILTIALLIATAFFIYVLLSTKFNHV
                     ALKGFDKAKYELNKYHAERYWGIFVAGILIWFWVLGYPWMPPVAFEKGLNETQDVHIV
                     KITAGQWYWKIEDGGYYNNNNNNNNNNNNNQSKTDLAS"
BASE COUNT         3300 a         1314 c         1379 g         3472 t
ORIGIN      
        1 aaatataaat tttttatatc attaaaggat tttttaggga acgtatcaca ataagattat
       61 taagagtgta aaataaagaa ttaacgacca taggtattac ttataatgtt tagaggataa
      121 taaatttaat cattattaaa ttgattatat agagtgatat ttatctacaa taaatattat
      181 tttctatcta ctaaaaaagt tttaataatg ataaattaga aaattcaata ttccaaatcg
      241 gggaaacttt ctaatttttg ttgattggaa caaatttaca tttaaattat ttagttacat
      301 acatatcttt ctctaaaatg gacatttcca ctatcgctta caaaaatatt tcctttggga
      361 tcaatagtta tatcatgagg tattaaaaat tgatcatctt tacatccaag ggaaccccac
      421 atggtaataa agttaccatt actatcaaat ttttgtattc ttacatttcg tgtatctact
      481 acatatacat attctgattt atctactgct atcccatgat cttcaataaa ttgtccttct
      541 ttacttcctt tagatcccca catggtaata aaattaccat tactatcaaa tttttcaact
      601 cttgaattat ttctaccatc agttacatat acattatctt ttgaatctac tgtaacatca
      661 tgaatatgaa caaattgtcc tgtttttgat ccatatgacc cccatttttt tataaaattt
      721 ccatcattat caaatttttg aataaccgga ttttcttgat ctccaacata aacatcgcct
      781 ttagaatcaa tacctatacc ccaaggtttc atgaattgat ttgtcccatt tccttttgat
      841 ccccacattg taataaagtt accatcactg tcaaattttt gaatatgatg tgtggcatag
      901 tcaactacaa acacattatc gtttttatca attgcaatac tctctggttg tatgaattct
      961 gctggcccac tacccttttt accaaactta agcaagtaat taccattact atcaaatttt
     1021 tgaatattgc atagtacagc atcacttaca tatacattac tgttagagtc agaagcaatc
     1081 ccatgaggat gcaaaaattc tcctttgcca gtgcctttat tcccccaact gtcaagtaaa
     1141 taaccattag aatcaaattt tttgatatga tgaaattttt ctagtgggtt aagactagta
     1201 acgttaacgt tattattaat attcttgcca ttagaattgg aaatcgcttt atctatcaat
     1261 ttttcatttt tacaattttc aattttattt atttctgttg gaaaatctgc atatgatgat
     1321 aaatgattca tgttatttat tagtgtatat gttataacaa agatcaataa aaataagact
     1381 agcttattat ctaatatttt atgtatcatg ttaatatcaa taatatatta tattacgatc
     1441 tataaataaa tacaaataat ggtattactt tctccattta tagaatcaac tcttattaac
     1501 aatatagttt taccttttat atggttaaaa ttgcaaatga tgtcaaattt caaaaaataa
     1561 ggttgaacga cagaagtaat tataattcaa gatatagacc tataacattt ctagcaatct
     1621 tggggttaat gatacttttt ggcattattg tttatcaaaa ttctgaagta ttagcaatag
     1681 aagaagaaac ttcaagtaat aataattatt ctttttctga gatatttaaa aaagtaaatc
     1741 aatcagtcgt acaagtatcc actactactg atcctaatcc taaccaatta ggggttaaac
     1801 ttggatctgg ttttatctat gatcaagatg gacatataat tactaattat catgtagtta
     1861 atggtggaaa tgaatttctt gttacttttc ctgatggatc aatatatagt tgtgaattag
     1921 taggttttga tccttatagt gatcttgcag ttattaaggt tatagtacct caagaaaaat
     1981 taataccttt aacgattgga aattctaaaa atttagttat aggacaacat gtagccgctg
     2041 tgggtaatcc atttggatta tctggttcca tgaccgaagg aattatcagt ggtttaggta
     2101 gacaattacc ttctttacct acagaatcac taccaccatt atttggagga aatgaaatta
     2161 cattacgatc atcatctttt tctatacctg acataatcca aactgatgcg gcaatcaatc
     2221 caggaaattc aggtggtcca ctgttaaata taaaaggaga ggtaattgga ataaattctg
     2281 caatattttc taatacggga gagtatgctg gtgtaggatt tgctattcct tctaatacaa
     2341 ttcaaaaaat tgttccttca ttaattgaaa ttggttctta tgatcatcca tggcttggaa
     2401 ttataggtgt agatattaca cctacaatag cagaagctat agatttgaat ctaactgatt
     2461 caaaaggatt tttaatcaca gatgttaatg caaatagtcc tgcagataaa gcaggaatac
     2521 aaggaggaga caggatagca aatatagacg ccaatagaga aatagactta ggaggagata
     2581 tccttattgg aattgatgga aaccctgtta gaaagataga tgatcttctt acatatttgg
     2641 aacgtgaaaa acgtgtagga gaaaaagtta ctgtggattt gattagaaat ggagaaactt
     2701 tggaaaaaac tttaactttg gaaaaaagac caaattcaga ggacatattc caaatcggca
     2761 atcagcaact ctccttagga gtaacaggga tagaagttaa tcccctatta gcagaggcac
     2821 taaatttaac tgattcaaaa ggatttttag taacaagtgt tagagcaaac ggtccagcag
     2881 ataaagcagg aatacaagga ggatataaag ttgagataat aaacaatatt ccatatacag
     2941 taggtgggga tataataatt ggaatagata ataaaactat caatacagtt caagatatca
     3001 aaaattatat taatagtaaa aaagaaggag acgtagttaa ccttaaagtt atacaagatg
     3061 gagaaattaa aaatattgat gcaaaattag aggtacttcc tattacatca agtgatgcaa
     3121 aaccttttaa taaattcaat ccatttggtg aagatagaga agaagattct tagagatttc
     3181 cctaataatc atctttcatt aagatctaaa aaaacaatgc tcacaaaaat ttaatggcat
     3241 gacaatagta aaacaaatga aagaaagtgt caaattttaa tagattaaaa atttcaatca
     3301 ggttagttat tagatgcata taatttagtc actaaatctt atcatctatt gttattctta
     3361 tttgctttcc ttctaagtct attatttggt catgaaactc ttttggaatc caaattactt
     3421 tattattacc catcatactg attctaccaa caaatttaaa tttggacaat tgaattagag
     3481 attaattcaa cactagatat ataacagtat actatagcca aattatggtc ttgttatgta
     3541 ggtatattaa gttgtgctac aactaaagat cttattatat attctatact atgtgaaatt
     3601 taataatttt taaccaattt gatagaacaa aagaaggaaa tttaagaaaa agaaagtgtg
     3661 gagagattat tttcaaattt tatttatgtc aacgaagaag ataaaagaat tactgttgct
     3721 ataatttaaa caaaaaagga agcatattat ttagaataag atcacacgtc aatgttaaat
     3781 acaatatcaa ataaaaaact aaagaatcta gtataattct ataattctat aaatattatt
     3841 ctttatcttt atcgccgtcg tctttatctt tatctttatc gccgtcgtct ttatctttat
     3901 cggctggagt aatttcccct tcagttatta tgatttcttc accttgttgg gcttgagtag
     3961 catttccagg ttgttgggct tgagtagcat ttccaggttg ttgtgctaat acgttttcgt
     4021 atattattgg tattcctgca attaaagcaa ctgcaagaat tgatgtcata acaatggcat
     4081 tcattttcga atccatatta catttagtta agtcttttat ataaatttta tttaaaattt
     4141 gttcattaat ttcctacact tataccatat ttactaattt gtactaatat ttgttgataa
     4201 taatcatttc tagctatata aaaataaaca agtaatttta gatataaaat tttgtatatg
     4261 acagattaag aaataatatc aactactatg ataatagtga tcaatggaat cgagtgtagg
     4321 ttgtttctca ctttctttct taactacatc tagaatctta ttccacatat gcttttcaaa
     4381 ttcatatgcc attattcccc caactgtagc tccagcagca agaccgatag gtcctccaat
     4441 tactaatcct acagcacccg tccacacaag acttccgaac catgcacctg atgctacttc
     4501 gacagcataa tcatttggat cacctgtaat ggatacatga atctcacgta aagatcctgc
     4561 ccataccttc ggagatttat ttacggctac aatactacgt aaaggaacag aattcataat
     4621 acctttatat tcggaaacaa tctcgagatc cttttggtct tgtagttctt gtttaatgtt
     4681 atcgtaaagt ctgtctaaat ttgtatcaag tcctcgataa tgttgtactt ttgccataaa
     4741 aataaggtag tcaagtatta tttaagcgtt aaaaagattt atgctttatc tttatcattc
     4801 attaattttt gatataataa gttacaatat tgtagattga taataattct ttactagtaa
     4861 cttttgtaaa ttgattgtat ataaatggat gatagatctg taatattgaa ttttaaccag
     4921 aaaaaaatta tattgtcatg tttagtttga tatttaatta ttatagtaat atcttcatgt
     4981 ttgactattg taaagtttat atttaactaa taagctatca aaaaatctat caaaaactcc
     5041 tttgtttatt atatcatctg agtataaccc taaagcaata acttttcctt tattataatt
     5101 atgttcatag gttgctacga ctatattttt gtatgagtta tccccaggat caataaaagg
     5161 atattttaac aaaataatat cagttggatt ggttatataa ttttcttcat gatggaaata
     5221 tccaaaagga ttatttagat atgatatatg acaagtagca catagataat tactacctac
     5281 ccattgagag gtttcatttt tccatctctc ttctacactt ttccatgcag actttccatt
     5341 aaaagcccaa gcatgacctt taacaagtgt tatttgattt gtacttttat catattttac
     5401 ctgaacatac aatgcatttc catccaaaaa gatcatcgtt cctccgttag aaacaaattc
     5461 tttcaaatta tcatattcat cctgagtgac atactcctga tgtcctagaa ttataatatc
     5521 ataaagatta gatccgtttt tattgaaaat taatttcgaa tccacctctt gatcggatag
     5581 tattgtgata ttggaattat ttgttatcca ttttaaattt tttattagat caagcattgc
     5641 atatacattc gaaattgagc cttcttgatt agaagagagt ttgcttgtaa gtaaattgag
     5701 attagtagta acatttgtat tagctggaac attttcatac attctataaa agacataaaa
     5761 ggcattatga taagcagcat cagtaaaagt gggtttaaca agagctattt ttgatttttc
     5821 aaattctaaa gtatggctat tgttttctgg agaatgtatt tcacttttat tcttggaaaa
     5881 aacatattga gaggcatttt tgccgattag gtttacatta ttagaataaa tagtttgatt
     5941 tggaagagtg gacgctgtat tattactact acttatttct cctacaccaa caagattttt
     6001 aatttcgttt aatggtaaag atgattgatt ttctgaaata ttgattttat taataggcat
     6061 tgattctgct tttaaataac taaaatatgt cggaagcaat tgttgaatat tgtttgattc
     6121 tataaattga taaggtatat tgttattatg attaatatac aatattgtga ataaaaaaaa
     6181 gatgccaaaa atgtttagta gactaatttt tagccttttc atctttattt taatctatgt
     6241 tactatagac attatgtatt tataaatata tattatattt tacatgttta atactataca
     6301 tcatacttgt aaaagagaat caaaacggat tcagtaatgt ataaccacgg tattagaaag
     6361 tacctatatg gaaaacatga ggtatatacg ggaaagaaat gaagtgatta ttacaaggtt
     6421 ttgtataatc aaagaaataa atttgaaaca attgtatctt tgacaaaaag acttttttga
     6481 gaatccaata aataaattgc atatgttgat gataatttcc aattaaacaa tctttttgtt
     6541 ctactcgcac gatttactct ctatatgaga ttaacaaata gaattctaat tattcttttt
     6601 atggtaaatc ttgtttgttg gattggcata gcattagatt attataatga taaaattctt
     6661 aatataggtg tacatttaat cctatttagt attattttta ctattggatt tattttagct
     6721 ttaagacaat ttactaatga agaaaacata tagatgtaat ctaattaata ttctcatatg
     6781 ccaaaaaggg atctttaaac tacaaacctt acaaccactc atttttatgt attgatatta
     6841 tccattagca ttgagtcgac ttttaaataa tattggaatt gttgaatatt atttgcttcc
     6901 aataaatgat aagatatatt actactatga agtaccattc aatggaacat ttatttcact
     6961 taattgatct cttatttcag tttcaatttc tcctaggttt tgtgatacta actcaagatc
     7021 accctctgcc aatcccgcat tagctttatc tacaaagttt agtatcttgg ttattgattc
     7081 ttttgggaat tctatactac tattggtttc ttgagcatat gcttgagttc catgataaaa
     7141 ttgaaagcct gcagaagcta gtactaaaat ggcagtaact attgttattg ttttaccgaa
     7201 actaattctc gatagtagat tcattagaaa ttactttaga attcaaatat ttaaaaatat
     7261 cgttataaaa tattaaaatt cttgaaactt atgtttttag attctagttt ttccaactac
     7321 tttaatctca aatattgtag ttacttctct ttaattagtc cagatgatac tccaaaaact
     7381 acattgtttt actaatagaa gttaagagcc tatccaaaaa tagatcatca gggatgatga
     7441 acccatttag ttaatgggac caagcagata tgaaaccaca ttatattact gcctaaaata
     7501 gagaagttaa tttgaagatg acaagatttt ggggcgtcga aaaattttgt tgttgttgtt
     7561 gttgctagta ttcacttttg aaattagtta tttatttttt tgtgtatacc aatatagttt
     7621 attattctgt tctctctttg catttaggta atctttcata aagaaggtga tgataaatta
     7681 aagtgaatag ttcctaaata atacagaaaa tttcatataa ccaaaaaagg ttatgtgtta
     7741 gggagtacaa caagttgtcc attcatcgtc ggttgatgat attgacaata atatctaaaa
     7801 attccaggtt cgtttgcggt aaatgaggta gagccattct taccttgacc caaatctaaa
     7861 tttatgttat aaggagcatt tattataaat gaatgtctat ctcccattgg ggcttccata
     7921 ttgtaaaagt atatagtaag attgtcattc tgattaactt ctaatgtctt tagcgagaat
     7981 tgatcatgtg ggtattctgt tacattaaaa gctggtaaaa atgtattgaa taagaagaaa
     8041 aatggttttt cggtctgaga agtttcttta gagtgttcat taacagttgg aatagaaatt
     8101 gatttagatt gactctggtc tgttgtagat gcattagttt tcaaaacttc actattagca
     8161 ttgataatat ttgattctgt attatttttt gtatttgacc ctactatgtt aagggaggct
     8221 ctcatccatg gatgtatggt acagccatat ggaacattcc cttcaccttt tacaatttca
     8281 gattcagatg atccagcttg tatgagtcca gtatcaaaag acccatcaga agcagttgct
     8341 gtatgtatcg tgatatcatt gtttatccag gtaattttag agttatcaga tatttcagca
     8401 ggattaggat caaaatattg cttaacctgt tccgcagcag aacctttagc gatatttatt
     8461 gaagtaataa cattattcgt tttacttgaa gattgagtag gtgtagttcc attttcattg
     8521 ggagaaactg gttgtattgt agtctgggag gttgcacttg attgtttatt tgagttgtct
     8581 aaagcaacac tattaataaa aatggcagat aaagcagata ttgctaaaac tattcctact
     8641 ccaaatataa cctgagaaag aatgttattc aattatacca gaagatcata gcgttattta
     8701 taaggatttt ctaatatata gacaaataat aaaatatcaa gtaaattttt ttcttatttc
     8761 agttttgatg actatattgt ggaaaaattt ccccaaggag atgataaaat taaagcgaat
     8821 agctcctaaa taatacaaat aatttattca actttattca tagctataga tttttcgtta
     8881 acttaagagg agcctctata tccaaaggca atttgactga ataaacatct ataaagaatt
     8941 taatttcatt gattatatta tttatcattg tttaaatttt atttgttttt atgaggaagg
     9001 taaaaaaact ttttatattt tgaaataact attgtaataa ttctaatgag tggcatagaa
     9061 cttgcagctg aattgggata tggagttcca tggttaaaac tcattttaac tatagcacta
     9121 ttaattgcta cggctttttt tatttacgtt ttactttcta caaaattcaa tcatgtcgca
     9181 ttgaagggtt ttgataaagc aaaatatgaa cttaataaat atcatgcaga aagatattgg
     9241 ggaatttttg ttgcagggat actgatttgg ttttgggtgt taggatatcc ttggatgcca
     9301 cctgttgcgt ttgaaaaggg attaaatgaa actcaagatg ttcatattgt aaaaataaca
     9361 gctggtcagt ggtattggaa aatagaggat ggtggctatt ataataataa taataataat
     9421 aataataata ataataataa tcaaagtaaa acagatcttg cttca
//
LOCUS       DASVEV010000147        15264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1219298, whole genome shotgun sequence.
ACCESSION   DASVEV010000147 DASVEV010000000
VERSION     DASVEV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15264
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219298"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF222_06755"
     CDS             <1..612
                     /locus_tag="VF222_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7142160.1"
                     /translation="AIRKITRKGCERVVKKAFELCKELDFKKIFVITKRNILKETDGI
                     FVSAVEKIKKDFNTIETEEYYIDNVTQQLVKNPQRFNKSILLSTNLFMDIISECASGH
                     VGSIGNVYSGNYGDKYAMFEPAHGSAPKYAGQNKVNPVATICSGAWMEEYLGEKDIAK
                     AIFKATEDVINENKYVTYDLGGNASLSQMGEQISQRASKLLKK"
     gene            complement(635..1159)
                     /locus_tag="VF222_06760"
     CDS             complement(635..1159)
                     /locus_tag="VF222_06760"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7142161.1"
                     /translation="MYIPDVDSFLLIDSIQNYFGQSALEIGTGSGIVSKYLLNNFKNV
                     VSTDLDYNSLLYCKKQNNSKISLICCDATTAISSKFDLIVSNPPYLPNDAFHDKTIHG
                     GINGIEKTIHFIRSAISNLRKCGYIILIVSSLSDISKLDRFIFEKKINRKIINKKKIF
                     YETLYVYELSIINL"
     gene            complement(1182..1895)
                     /locus_tag="VF222_06765"
     CDS             complement(1182..1895)
                     /locus_tag="VF222_06765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX7142162.1"
                     /translation="MNWRKYYGQTFLIDLRVAREIIERSEVKSFEDVCEMGSGKGILI
                     PYLCQKAQFVKSFEVDYKLYNKIKILQSKFNNLEIINEDILKHIKPLKFDVFVSNIPY
                     SKSKEIFLWLATNKFDRATLMVQDEFSQKIQSKTGEKNYRAVSAISQYCFNIQPLFKV
                     DRKSFYPKPNVDSQVIKLTSKNKLLSKDTISKIHLIFSFKNKNVSKLNNKLNTNFNEI
                     NLRVKEMEPEKIIALSKLI"
     gene            complement(1892..2497)
                     /locus_tag="VF222_06770"
     CDS             complement(1892..2497)
                     /locus_tag="VF222_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HEX7142163.1"
                     /translation="MSLSDHYQDSKGNAFGSFHVPRRYEEYAHIIEFTIRGKSSTVKK
                     REGIIIQAIGEERLTLLELLGDPSQDFEIGERAYIGREGRNKIISVLGRLNYNDLSVS
                     AKNEIPIAIEKIVNIKEKRFVDYFNNAQPITPRVHGLELIPGIGKIYMKSIITEREKK
                     KFDSFTEIQQRVGVKDLQKLIAKRIVEEISGETRMNLFVRK"
     gene            complement(2521..2853)
                     /locus_tag="VF222_06775"
     CDS             complement(2521..2853)
                     /locus_tag="VF222_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4"
                     /protein_id="HEX7142164.1"
                     /translation="METLKKEVISLAEVKDILEKVETEEMDQIQRWTFDYVSKFAKSP
                     SQVSRTSINELVASCDLKEEEAVELANAMPTSIEELRAFTFGWKKLILTDTLNKILDI
                     LNKDKKVN"
     gene            complement(2865..3149)
                     /locus_tag="VF222_06780"
     CDS             complement(2865..3149)
                     /locus_tag="VF222_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142165.1"
                     /translation="MPRSHGSKRKSRSILTKSNILRGISYLLINYKIGDKVIVNIDPS
                     EHNTMPHRRYQGKIGIIKEIGKRIIKVSIAIGNKEKILQTKRNHLKPLSN"
     gene            complement(3192..4172)
                     /gene="radA"
                     /locus_tag="VF222_06785"
     CDS             complement(3192..4172)
                     /gene="radA"
                     /locus_tag="VF222_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HEX7142166.1"
                     /translation="MINKDNNSKKLELDTLDDIGPATKIHLVEAGIDSLKELIIRGPH
                     DVAEVTGMSMEKSIDLCNKARIKLEEVNMIEKTFVPATELYNKRKKIERISTGSKNFD
                     DLLGGGFEVNAITEIYGEFGSGKTQICHTSSVMVQQKRTEGGLEGGVIYIDTENTFRP
                     ERIVSISKARNIDYASVLENIVVAKAFNSAHQELIIHEIGKIIENHNIKLLVLDSAIS
                     HYRAEYLGRGTLSERQQKINRLMHVLIRISETYKIAVILTNQIQSVPDTIFGDPFRPA
                     GGNIIAHSSTYRIYLKKAGKNRIARIVDSPYHPEIEALFSLGEEGVTDPI"
     gene            4315..4992
                     /locus_tag="VF222_06790"
     CDS             4315..4992
                     /locus_tag="VF222_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX7142167.1"
                     /translation="MIVDLNLAGKNVIVIGGGVEGIRKVRGLLGQNCKITVISERLNS
                     YLEELSQEKKIKIVKMKITNANVLDSYRDIFLVLAATDNKELNRKIVEKGRSMMSFVY
                     AADDPQVSDFSYASIINIEGIMQIAISTSGKSPIMARKLRIKAERILRRTIKQSDIEN
                     TKLQEFARMAARPKIKTVIERKRFLYSLINDKNIQNLINENKIDEAKVAAIELLNHWS
                     DLKNDSR"
     gene            4979..6274
                     /gene="hemA"
                     /locus_tag="VF222_06795"
     CDS             4979..6274
                     /gene="hemA"
                     /locus_tag="VF222_06795"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7142168.1"
                     /translation="MTLVNKSFDLLNFRITYKEAPIHTLEKFAFKDINSAHETFLEHL
                     QMKECLILQTCNRVEIFAVIEEELDLQKIIGYWADHSKFSTEEFSKIVEIVRGEEVIR
                     HLLKLVAGLDSLVIGEDQILGQVKRSYEFSRSNNYLGSFLSIIFEKSIRVGSKVRLLT
                     GINKGRVSVGSVAVSLAEESIGRLDDKKILLIGSGEGASLIAKALKQRNVSFMNTSRT
                     FERARSFAESLSGIPILFEDALTSLNSMDIIFVSTTAPYYLVTHDRIKKTMETKNTSL
                     LIFDLSNPRTVEDGISQINNVSLFNLDQIGDIVQRNLQWRKEEIQSADKIIEEEMKNM
                     DLLLKRRKADPVVVSVFRNVDKIRKRELNKALSILGPKIGPQEGKIIEQFSHALLEGI
                     ISTPMNNLRRQIQNDKNDEQELIRLAAKLFNYENEANIQ"
     gene            6255..7271
                     /gene="hemB"
                     /locus_tag="VF222_06800"
     CDS             6255..7271
                     /gene="hemB"
                     /locus_tag="VF222_06800"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7142169.1"
                     /translation="MKQISNKSLANGSYFPNIRLRRLRKSKALRDLLQETRISSDDLV
                     FPLFVEEGLKYSKNVASMPDIQRIPPSKIVDVVSEILDLGIKAVVIFGIPANKDYAAK
                     SAYNEKGVVQKSIRTIKKHFGDRIVVISDVCMCQYTPSGHCGIVKEKKVDNDASIEVL
                     ANIAESHALCGADIVAPSAMMDGQVFAIRERLNDAGLNDTSIMAYSAKMASPLYTPFR
                     DLADSTPSFGDRKTYQMPFTNPLESLREIELDIKEGADMIMIKPALPYLDLICKARES
                     FTLPLCSYSVSGEYALIKAAAEKGWINETDVMIEFLSSIKRAGSDLIITYFAKKMAKV
                     LLEK"
     gene            7305..8585
                     /locus_tag="VF222_06805"
     CDS             7305..8585
                     /locus_tag="VF222_06805"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7142170.1"
                     /translation="MTSNRSEKLFQRAIKVIPGGVNSPVRYFKPYPFFVSSGLGSKIV
                     TADHETYLDYCMGYGSLIFGHSYLPIIESVKMQLDNGTLFCIPSEKEIHLAELMTKII
                     PCAEKVRLVNTGAEATMTAIRLSRAFTGRNKIVKFNGGYHGAYDYVLVKAGSGAANIP
                     SSAGSLQEISKETLIVQYNDFGELERLIEKESEHISCLIMEPVLANYGLIIPEKNYLT
                     QIRKITKKYGILLIFDEVITGFRLSVGGCSEFFGVTPDIATYSKSFGNGFPIAAICGK
                     KEIMDQLTPSGNVYQASTFAGNPVSVTAAITTVESLLKNKNIVYPTIARLCDTLVMGI
                     KDRVKDFDNYYTLNSIGSLFQIFFTAETVSTPLDILKSDQKLFENLFWDLLKKNIFIP
                     PSLYETCFISYSHCDEDIEKTIECYADSLNKYIK"
     gene            8587..9540
                     /gene="hemC"
                     /locus_tag="VF222_06810"
     CDS             8587..9540
                     /gene="hemC"
                     /locus_tag="VF222_06810"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7142171.1"
                     /translation="MTLKLLVGTRGSKLALAQTQIVIKSLSELNEPFQYEITPIKTKG
                     DLDNSKPLFYMDKKGIFEKEINELVVKGDIDFAIHSIKDVPAAIHPDLVIASIPKRES
                     VNDVLISKNRWKLNQLPAGSIIGTSSLRRAIQIISFRNDFKVIPLRGNIDTRIEKALK
                     GEYDAIVLAEAGLRRLGKEQYISERFDPKKFLPAAGQGALALVCKKNNRKLVRFLRKI
                     EHAPSRISINAERSFISKIEGGCRFPIAAYANLSPKNHKINFYAKIFSSDGLEHITIK
                     KIGDYDKPEIVGNMVANYLIEKGALRLAEGWDKSLNEWNKK"
     gene            9521..10282
                     /gene="cobA"
                     /locus_tag="VF222_06815"
     CDS             9521..10282
                     /gene="cobA"
                     /locus_tag="VF222_06815"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX7142172.1"
                     /translation="MNGIKNKKNGKVYICGAGPGDPSLITMRCWDLIKKCDVILYDRL
                     VSNGILKLIPKDKAKVYVGRTSGDPTTNQFKTNQIMLQYALEGKKVLRLKGGDPFIFG
                     RGGEEAEFLNENAIDFEIIPGISSAIGAAIYSGIPLTHRKYSSSIAIVTGHEDPMKEE
                     KTVRWDKIATSVDTIVILMGVERLESILKKLQKYGLSEETKIAVIEKGTLKDQKIILG
                     NFNNIKKKIENAAIKPPAVIIIGEVVSLSERIRWI"
     gene            10273..11133
                     /locus_tag="VF222_06820"
     CDS             10273..11133
                     /locus_tag="VF222_06820"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7142173.1"
                     /translation="MDLNDHRALDDTAKIIAITKSEGSISNKFIDQIEKRNCRLIVVK
                     TSKIVKCDSYIVTEIFNLISKRSSGYIIFLSSNAVDIFFEIATSLNVINEIVNDLNSK
                     FTVVAIGPSTRSALLKNRIVESKIPQDHSSLGIIDFLSKLKKDNYESKIIIPRSKKAD
                     NNLKEKLLHLGFDVDEFYIYNVIPADVNPGWIEFFNLLREEKIDSLIFTSPSNVDYFI
                     SIIQKYSSDLLPLVCKIKLILSIGPLTSKELFKHNLPFHESKNHSLEGLYETLYRDNK
                     NLNKTSNPFY"
     gene            11194..12594
                     /gene="sufB"
                     /locus_tag="VF222_06825"
     CDS             11194..12594
                     /gene="sufB"
                     /locus_tag="VF222_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7142174.1"
                     /translation="MTTEELNMDYSKYDFKDSTDLYVYTSKKGLSRGTVEEISRIKNE
                     PEWMLDFRLRSYDIFMKKPMPVWGGDLSKINFQNIFYYTKASEKQSKSWDDVPDSVRK
                     TFDKLGIPEAEKKFLAGVGAQYESETVYHNLREDLSKQGVIFSDTDTAVKEHPDILKK
                     YFGKVIPPEDNKFAALNSAVWSGGSFIYVPPNVKIDLPLQAYFRINAENIGQFERTLI
                     IADEGSEVHYIEGCTAPVYTTESLHSAVVELIAKKNAKIRYTTIQNWSKDVYNLVTKR
                     AYAYENARVEWIDGNIGSKLTMKYPGVYMLGRNAHAEVVSIAFAGSDQHQDAGAKAVH
                     LAPDTTSRITSKSVSKQSGRTTYRGLLHVAKGATGVKSNVRCDALLLDETSRTDTYPY
                     VEVNEDDATISHEASVGKIGEDQIFYLMSRGFSESDALSLIVGGFIEPFTKELPMEYA
                     VELNRLVKLEMEDSVG"
     gene            12632..14056
                     /locus_tag="VF222_06830"
     CDS             12632..14056
                     /locus_tag="VF222_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX7142175.1"
                     /translation="MYQLSLLEKNFDSYIENFKEPLWMKQTRYEAFKNYASLPLESSS
                     LYSKYSNANKLIADKVFLNDTNLETKSSSNDLDIFSDRIDEFSKTTSVLCKNSKILES
                     FVTDELEKKGIIILNISQALSDYSDLIQKYLNKNKLNYLEDKFLALSSAAFQNGLFIF
                     IPKNIVLEDPIRIVNYLSEDGLSSISRNIVVCEEFSKATLVQELYSPKLSSSKYQQTH
                     FELLESHIMPNAQCEIVTLQSMDYDAISFSNRKAYVERDGKMSWSLALFGSQLSRYKV
                     DSIMQGQGALAEDVEIIFGIGNQSFDITSNLIHVAPHTRGKVLVKSVMKDTSKSLFKG
                     MIKIHKNAKATESYLAGHAILLDKGAKSDAIPGLEIESNEVKATHSASVAQIDEKQIF
                     YLMSRGLSEELAKREIVGGFLEPISRKMGPTIRAWINYLIENKWLGKPLKLKIDEAME
                     QILEIEKSRYRETEDIFEKHYKYR"
     gene            14091..14405
                     /locus_tag="VF222_06835"
     CDS             14091..14405
                     /locus_tag="VF222_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX7142176.1"
                     /translation="MSNWVRVCNIDSLKPGELMDFDFNDKKILLVNIKNKIYASDRIC
                     THAYADLSMGFLNEEEKTVTCPLHMSAFNLKDGLPLNLPAEEPLTVYETKIDNNSVFI
                     FV"
     gene            14666..>15264
                     /locus_tag="VF222_06840"
     CDS             14666..>15264
                     /locus_tag="VF222_06840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7142177.1"
                     /translation="MKIGIIGLGLMGSNLCLRLIRKGFQVNVNNRDLSKSLLLKKKGA
                     KLFNTPKDVADYSDFIITCVTNFEAVNRIFFHENGVSETENKNVIVSDFSTVSPKESV
                     YCYQKFKENNLSMLSIPVMGGPNAALHGKLIPIVSGEETSFKKIKNVLEELGDPIFYI
                     GNKLGSANAIKLALNLNIGIIAMALSEGLLLADKYEIDP"
BASE COUNT         5383 a         2219 c         2509 g         5153 t
ORIGIN      
        1 gctattagaa aaataaccag aaaaggatgt gaacgtgtgg ttaaaaaagc atttgaatta
       61 tgtaaggaat tagattttaa aaaaatattt gttattacta aaagaaatat tttaaaagaa
      121 accgatggaa tatttgtttc agcagtagag aaaatcaaaa aggactttaa tactatcgaa
      181 acagaagaat attacataga taatgttacc caacaattag tcaaaaatcc tcaaagattt
      241 aataaaagta tattattaag tacgaactta tttatggaca taatatctga atgtgcttca
      301 ggacatgtcg gatcaattgg caatgtttat tctggcaatt atggggataa atatgctatg
      361 tttgaacctg ctcatggaag tgcaccaaaa tatgcaggcc agaataaagt aaatccggta
      421 gctaccattt gctcaggtgc ttggatggag gaatatttag gtgagaaaga tatagccaaa
      481 gctattttta aggctactga agacgttata aatgaaaata aatatgttac ttatgattta
      541 ggtggtaatg catcactctc ccaaatgggc gaacaaattt ctcaaagagc atcgaaatta
      601 ttaaaaaaat gattagactc aaatacatgg ctatctatag atttatgatg cttaattcat
      661 atacataaag agtttcataa aatatttttt tcttgttaat aattttccta ttaatttttt
      721 tttcaaaaat aaatctatca agctttgata tatctgataa agaagacaca attaaaataa
      781 tatatccgca ttttctcaaa tttgatatgg cagaacgaat aaaatgaata gttttttcta
      841 ttccatttat tcctccatga atggttttat catgaaatgc gtcatttggt aaatatggag
      901 gattacttac aatcaaatca aacttactag atattgctgt tgttgcatca caacatatca
      961 aagatatttt agaattgttt tgttttttac agtataacag agaattatag tctaaatccg
     1021 tacttaccac atttttaaaa ttatttaaga gatatttcga aactattcct gatccagttc
     1081 caatttctag tgctgattgt ccaaaatagt tctgaattga atctattaat aagaaactat
     1141 caacatctgg gatgtacatt taagttaatt tcaagtacgg ctcaaatgag tttggaaagt
     1201 gctattattt tctctggttc catttctttt actctgagat ttatttcgtt aaaattagta
     1261 ttaagtttat tatttaattt acttacattt ttatttttaa aagaaaaaat taaatgaatt
     1321 ttagaaattg tatcttttga aagtaattta ttttttgatg ttaattttat tacctgtgaa
     1381 tcaacgttag gtttggggta aaatgatttt ctatctactt tgaataatgg ttgaatatta
     1441 aaacaatatt gtgatatagc tgatactgct ctataatttt tttctccagt tttcgattga
     1501 attttttggc taaattcgtc ttgtaccatt aaagtagcac gatcaaactt gtttgttgct
     1561 aaccataaaa atatttcttt acttttagaa tatgggatat tagaaacaaa tacatcaaat
     1621 ttcagtggct ttatatgttt taaaatatct tcatttataa tttctaaatt gttaaatttt
     1681 gattgtagga ttttaatctt attgtataat ttgtaatcaa cttcaaatga ttttacaaat
     1741 tgggcctttt gacataagta tgggataagt atcccttttc ccgaacccat ttcacaaacg
     1801 tcttcaaaag attttacctc tgatctctct attatttctc tggctactct taaatcaata
     1861 agaaaagttt gtccataata ttttctccaa ttcattttct aacgaataga ttcattcttg
     1921 tctctcccga aatttcctct actattcttt ttgcaattag tttctgtaga tcttttacac
     1981 ctacacgttg ttgaatctca gtaaaactgt caaatttttt cttttctcgt tcagttataa
     2041 tacttttcat atatatttta ccaattcctg gaattaattc taatccatgg acacgcggag
     2101 taataggttg agcattatta aaatagtcga cgaatctttt ttccttgata ttaactattt
     2161 tctctattgc gattggaatt tcattttttg cagagactga aaggtcatta taatttaacc
     2221 tacctaaaac actgattatt ttatttcttc cctctctccc aatataggca cgttctccaa
     2281 tctcaaaatc ttgagatgga tcgccaagta attctaacaa tgttaatcgt tcttctccaa
     2341 tcgcttgtat tattatgcct tctctctttt ttacggttga agattttcct cttattgtaa
     2401 attctataat atgtgcatac tcttcatatc ttcgaggaac gtgaaaacta ccgaatgcat
     2461 ttcctttact atcttgataa tgatccgaca gtgacatgaa tattcttcct ttaaaatgct
     2521 ctaattaact tttttatctt tatttaaaat atctaaaatt ttatttaatg tatcggttaa
     2581 gatcaatttt ttccatccaa atgtaaaagc ccgtaattct tcaatagaag tgggcatagc
     2641 atttgctagt tctactgctt cttcttcctt taaatcgcaa gatgctacta attcatttat
     2701 actggtcctt gaaacctgtg aaggagattt agcaaatttc gaaacataat caaaggtcca
     2761 tctttgaatt tgatccattt cttctgtttc tactttttct aaaatatctt tgacctctgc
     2821 gagggatata acttcttttt ttaatgtttc cattcaagtc tcccctaatt acttaaaggt
     2881 tttaaatggt ttcttttcgt ctgtaaaatt ttttctttat tgcctatagc aatagatact
     2941 ttaataattc tttttccaat ttccttaatt attcctattt ttccttgata tcttctatga
     3001 ggcattgtat tatgttcact aggatcaata ttaacaatta ctttatctcc tattttgtaa
     3061 ttgattaata aatatgaaat acctctaagt atattacttt tagttaatat agatcgtgat
     3121 tttctcttac ttccatgtga tcttggcata ttatatgtga attacatcct taatttaata
     3181 ttaactgtat tctatatagg atcagttact ccttcctcac ccaatgaaaa caatgcttca
     3241 atttcagggt gatatggact atcaacaatt cgagcaattc tgtttttgcc agctttcttc
     3301 aaatatattc tatatgtact agaatgtgca ataatatttc ctcctgctgg ccgaaaagga
     3361 tcaccaaata tagtgtcagg gaccgattga atctggtttg ttaatataac tgctattttg
     3421 taagtttcag aaatcctaat taatacatgc attaatctat ttattttttg ttgcctttcc
     3481 gataacgttc ctcttccaag atattctgca cggtaatgcg aaattgctga atctaatact
     3541 aataacttta tattgtggtt ctcaataatt tttcctattt catgaattat tagttcttga
     3601 tgtgcactgt taaatgcttt tgcaactact atattttcta atacgcttgc atagtctata
     3661 tttctagctt tagagattga tactattctt tctggtctaa aggtattctc tgtgtcgata
     3721 taaattactc ctccttcaag cccgccctct gttctttttt gttgaaccat tactgaagag
     3781 gtgtgacaga tttgagtttt tcctgacccg aattctccat atatctcagt tattgcgtta
     3841 acttcgaatc ctcctcctaa caggtcgtca aaatttttag aaccggtaga aatccgttct
     3901 atcttctttc ttttattata aagctctgtt gctggaacaa aagttttttc aatcatattt
     3961 acttcttcta gtttgattct agctttatta cacaaatcaa ttgatttttc catactcatt
     4021 cctgtaactt cggcaacatc atgagggcct ctaattatca attcctttaa tgagtctatt
     4081 ccagcttcaa caagatgaat ttttgttgca ggcccaatat catctagagt atcaagttct
     4141 aattttttag aattattatc tttatttatc aattgagatc attttgaatt atatatatta
     4201 attcttgaac taaatatttt tgtgtattta tacaattcga ttctataact tctatttact
     4261 tgaaataatt taaataaagt aatcaatatt gtttcatcag cagtgaaatt ataattgatt
     4321 gttgatctaa atctcgctgg aaaaaatgta attgtcattg gaggaggagt agaaggaatc
     4381 agaaaggtca gaggattatt aggtcaaaat tgtaaaatca ctgttattag tgaaagattg
     4441 aattcttatc ttgaagagtt atcacaagaa aagaaaatta agatagttaa aatgaaaatt
     4501 acgaatgcta atgtattaga tagttatcgt gatatttttt tagtgcttgc tgcaacagat
     4561 aataaagaac ttaatagaaa aatcgtagaa aaaggacgat caatgatgtc ctttgtttat
     4621 gctgcagatg atccacaagt aagtgacttt tcatatgcct ctataataaa tattgaggga
     4681 ataatgcaaa tagcaatttc tacctcagga aaaagtccga taatggctcg aaaattgaga
     4741 ataaaagcag aacgtatttt aagaagaaca ataaagcaat cagatataga aaataccaag
     4801 ctacaggagt ttgcgagaat ggctgcaaga ccaaaaatta aaacagttat agaacgtaaa
     4861 agatttctat attcactaat taatgataaa aatattcaaa atttaataaa tgagaacaaa
     4921 atagatgaag caaaggttgc tgcgattgaa ttgttgaacc attggagtga tttaaaaaat
     4981 gactctcgtt aacaaatcat ttgatttact caattttagg ataacatata aagaagctcc
     5041 tatacatacg cttgaaaaat ttgcatttaa agatattaat agtgcccatg aaacttttct
     5101 tgaacattta caaatgaaag aatgtctaat tttacaaact tgtaatcgtg tagagatttt
     5161 tgccgttata gaggaagaat tagatttaca gaaaattatt ggttattggg ctgatcattc
     5221 aaagttctca acagaagaat tctctaaaat agtagagatt gtcagaggtg aagaagtaat
     5281 tcggcatctt ttaaaactag ttgcaggttt agattctttg gtaattggag aggatcagat
     5341 tctaggtcaa gtaaaaagat catatgaatt ttcccgatct aataattacc ttggatcttt
     5401 tctttcaatc atttttgaaa aatctataag ggtgggaagt aaagtgcgat tactcacagg
     5461 gatcaataaa ggacgtgttt cagttggatc tgtagcggtt agtctagcgg aagaatcaat
     5521 tggaagattg gatgacaaga aaatattgct tataggctca ggagaaggtg caagtcttat
     5581 agccaaagca ttaaaacaga gaaacgtttc ttttatgaat acaagtagaa ctttcgagag
     5641 agctaggtca tttgcagaat ctctttcagg tataccaata ctgttcgaag atgctttaac
     5701 ctctttaaat tctatggata ttatttttgt aagcactaca gcaccatatt acttagttac
     5761 gcatgatagg ataaagaaaa caatggaaac aaaaaatact tctttattaa tttttgatct
     5821 atcaaatcct agaacagtag aagatggtat ttcccaaatt aataatgtct ctctatttaa
     5881 tctagatcaa attggagata ttgttcaacg aaatcttcaa tggagaaaag aagaaattca
     5941 gtctgcagat aagataattg aagaggaaat gaaaaatatg gatttgttat taaaacggag
     6001 aaaagcagat ccggtagttg tatctgtttt tcgtaatgta gataaaatta ggaaaagaga
     6061 actgaataaa gctctttcta tacttggacc taaaatagga cctcaagaag ggaagattat
     6121 tgaacaattt tctcatgcac tcttagaagg aataatatct actcctatga ataatcttcg
     6181 tcgacagatt caaaacgata aaaatgatga gcaggaactt attcgcctag ctgcgaaact
     6241 gtttaattac gaaaatgaag caaatatcca ataaatctct agctaatgga tcatattttc
     6301 cgaatattag actaaggcgt ctaaggaaaa gtaaagcact ccgcgatctt ctacaagaga
     6361 ctcgtatctc gtcagatgat ttagtttttc cattatttgt tgaagaaggc ttaaaatatt
     6421 caaaaaatgt agcttcaatg ccagatatac aaagaattcc tccttcaaag attgtggatg
     6481 tagtttcaga aattttagat ctcggaataa aagctgttgt aatttttgga attcctgcta
     6541 acaaagatta tgccgcaaaa tcagcatata atgaaaaagg agtagttcaa aaatctatta
     6601 gaacaataaa gaaacatttt ggagatagaa ttgtagtgat atctgatgta tgtatgtgtc
     6661 agtatacacc ttcggggcat tgtggaatag ttaaagaaaa aaaggtggat aatgatgcga
     6721 gtatagaagt actcgcaaac attgcggaga gtcatgctct ttgtggagca gatatcgttg
     6781 ctccatccgc tatgatggat ggtcaagttt ttgcaattag agaacggctg aatgatgctg
     6841 gtttaaatga tacatctatc atggcttatt cagcaaaaat ggcgtcgcca ttatatacac
     6901 catttaggga ccttgctgat tctactcctt catttggaga tagaaaaacc tatcaaatgc
     6961 catttaccaa tccacttgaa tctttaagag aaattgaatt agatatcaag gaaggagctg
     7021 atatgattat gataaaacct gctcttccct atttggattt aatctgcaaa gcaagagaga
     7081 gtttcacact tcctctatgt tcatacagtg tgtccgggga atatgcctta ataaaagcgg
     7141 ccgctgaaaa aggatggata aatgagacag atgtcatgat agaatttctt tcctcaataa
     7201 aaagagcagg ttctgatctt ataataacat attttgcaaa aaaaatggca aaagtgttat
     7261 tagaaaaata aggttcttat aaatagattg taataattac atatttgaca tccaatcgat
     7321 cagaaaaatt atttcagcga gcaattaagg tcattcctgg tggggttaac agtcctgtac
     7381 gatactttaa accttatccg ttttttgtct cttcaggttt gggaagtaaa atagttactg
     7441 ctgatcacga aacatatctg gattattgta tgggatatgg gtctttaatc tttggacatt
     7501 catatttacc cattattgaa tcagtaaaaa tgcagctcga taatgggact ttattttgca
     7561 ttccatcaga aaaagagata catcttgctg aattgatgac aaaaataatt ccctgtgcag
     7621 aaaaagtaag attagttaat acaggggctg aagcaacaat gactgcaata cgattaagta
     7681 gagctttcac cggacgaaac aagatagtta agtttaacgg agggtatcat ggagcatatg
     7741 attatgtgtt agtaaaagct ggatctggag ctgcgaatat tccatcatct gctgggtcac
     7801 ttcaagaaat ttcaaaagaa actctgatag tccaatataa tgactttgga gaattagaaa
     7861 gactaattga aaaagaatca gaacatatta gttgtttaat aatggaacct gtcttggcga
     7921 actatggtct tataattcca gaaaaaaatt acttgaccca aattagaaaa attacaaaaa
     7981 aatatggcat tttgctaatc tttgatgaag ttataaccgg ctttaggtta tctgtaggtg
     8041 gatgcagtga attttttggc gtaacacctg atattgctac ttactctaaa tcttttggaa
     8101 atggatttcc aatagctgca atttgtggca agaaagaaat aatggatcaa ttaacaccat
     8161 ctggaaatgt ttatcaagca agtacatttg caggaaatcc cgtttcggta acagcagcaa
     8221 tcacaaccgt agaaagcctt ttgaagaata aaaatatagt atatccaaca atagctagat
     8281 tatgcgatac tttagtgatg ggaataaaag atcgcgtaaa ggactttgat aactattaca
     8341 ctctgaattc tattggatct ctattccaaa tattttttac tgcagagaca gtatctactc
     8401 ctcttgacat tttaaagtct gatcaaaagt tatttgaaaa tctattttgg gatttactaa
     8461 aaaaaaatat ttttatccca ccctcactct atgaaacctg ttttatttct tattctcatt
     8521 gtgatgaaga tatcgaaaaa actattgaat gttatgctga ttctttaaat aaatatatta
     8581 aataaaatga ccctaaaact acttgtaggt acaagaggaa gtaaattagc tttagcacaa
     8641 acccaaatcg taattaaatc tcttagtgaa ttaaacgaac ctttccagta tgaaattact
     8701 cctatcaaga caaaggggga tttagataac tctaaaccgc tattttatat ggataaaaag
     8761 ggtatttttg aaaaagaaat aaatgaatta gtagttaaag gagatattga ttttgctata
     8821 catagtatta aagacgtccc agctgctatt catccggatt tagtcatagc atctattcct
     8881 aaaagagaat ccgtaaatga tgttctaatc agtaaaaata gatggaaatt aaatcaactc
     8941 ccagccggtt ctatcatagg tacaagcagc ttaagaagag caatacaaat tatttcattt
     9001 agaaatgatt tcaaggtcat ccccttacgg ggaaatatcg atacaagaat tgaaaaagcc
     9061 ttaaaaggag agtatgatgc aattgttctt gctgaggcag gtttgaggag attgggaaaa
     9121 gaacaatata ttagtgaaag attcgatcct aaaaaatttt taccggctgc gggtcaagga
     9181 gcactagcac tagtgtgtaa aaaaaacaat agaaaactag ttagatttct tcggaaaatt
     9241 gaacatgctc catccagaat ctcaattaat gctgaaagat cttttatatc aaaaattgag
     9301 ggtggctgta gatttccaat agcagcatat gctaatttgt ctcccaaaaa tcacaaaatt
     9361 aatttttatg caaaaatatt ttcttctgat ggattggaac atattaccat taaaaaaatt
     9421 ggtgattatg ataaaccaga aattgtgggt aatatggttg ctaattattt aatagagaaa
     9481 ggggctttaa ggttagctga aggctgggat aaatctctaa atgaatggaa taaaaaataa
     9541 aaaaaatgga aaagtataca tctgtggagc aggtccgggt gacccatcac tcattacaat
     9601 gagatgctgg gatttaataa aaaaatgcga tgtaatatta tatgatcgct tagttagtaa
     9661 cggaattctt aaattaattc ctaaagataa agcgaaggta tatgtcggac gtacatcggg
     9721 ggatcctaca actaaccaat ttaaaactaa tcaaataatg ttacaatatg cattagaagg
     9781 aaaaaaagtt ttaagactta aaggaggtga tccttttatt tttgggcgag gaggcgaaga
     9841 agcagaattt ttaaacgaga atgctattga ttttgaaatt atccctggga tatcttctgc
     9901 aattggcgca gcaatttatt ccggtattcc acttacacat agaaaatatt cttcgtcaat
     9961 tgcaatagtt acgggtcatg aggatccaat gaaagaagaa aagactgttc gatgggataa
    10021 aatagcgacg agtgtcgata ctatagtgat tcttatggga gttgaaaggt tggaatctat
    10081 tctaaaaaaa ttacaaaaat acggtctttc ggaagaaact aaaattgctg taattgaaaa
    10141 gggaacccta aaagatcaaa aaataatact tggtaatttc aacaacataa aaaaaaaaat
    10201 tgaaaatgct gctataaaac ccccagccgt gattatcata ggtgaagtgg tatcattatc
    10261 agagcgaata agatggatct gaatgatcac agagcactag atgatactgc caagatcatt
    10321 gcaataacaa aatctgaagg ttctatttca aataaattca ttgatcaaat tgaaaaacgt
    10381 aactgtaggc taatcgtagt taaaacgtcg aaaatagtaa aatgtgattc ttacattgtt
    10441 actgagattt ttaacctgat ttcaaaaagg tcctctggat atattatttt cttaagctct
    10501 aatgcagttg atatattttt tgaaattgct acttctttaa atgtaattaa tgaaatagtt
    10561 aatgatttaa attctaaatt tactgttgtt gctattggac catctactag aagtgcactc
    10621 ttaaaaaata gaatagtcga aagtaagatt cctcaagatc actcatcttt aggaattatt
    10681 gatttccttt ccaaattgaa aaaagacaac tatgaatcca agataattat tcctcgaagt
    10741 aagaaagcag ataataatct aaaagaaaaa ctcttacatc taggttttga tgttgatgaa
    10801 ttctacattt ataatgtaat ccctgctgat gtaaatccag gatggattga attttttaat
    10861 cttcttagag aagaaaaaat tgactccctg atttttacaa gtccgtctaa tgttgattat
    10921 ttcatatcta tcatacagaa atactcttct gatctgttac ctcttgtctg taaaataaaa
    10981 ttgatattat ctattggacc tcttactagt aaagagcttt ttaaacataa tttaccattt
    11041 catgaatcca aaaaccattc attagaagga ctttatgaaa ctctatatag agataataaa
    11101 aacttaaata agacatctaa tcccttttat taatcctttt ttaatagtat ttggattgta
    11161 aaatttatat aacataacgc cgttatgaaa gatatgacta cagaagaact taacatggat
    11221 tatagtaaat atgattttaa ggactctact gacttatatg tatatacaag taagaaaggt
    11281 ctttcacgcg gaactgtcga agaaataagt aggattaaga atgaaccaga atggatgctc
    11341 gattttagat taagatctta tgatattttc atgaaaaaac ctatgcctgt atggggtggt
    11401 gatttatcaa aaattaattt tcaaaatata ttctattata caaaagcttc agaaaaacaa
    11461 agtaaaagct gggatgatgt gcctgactct gttagaaaaa catttgataa actcggaata
    11521 ccagaagcgg aaaaaaaatt cctagctggt gtgggtgctc aatatgaatc agaaactgta
    11581 tatcataact tgcgtgaaga tctatcaaag caaggggtaa tattttcaga tacagataca
    11641 gcggttaaag aacatcctga tatcttaaaa aagtattttg gaaaggttat accaccagaa
    11701 gacaacaaat ttgctgcatt aaatagtgcg gtatggtcag gtggttcttt tatttacgtt
    11761 ccacctaatg taaaaattga ccttccatta caagcttatt ttagaattaa tgcagaaaat
    11821 ataggacaat ttgaacgtac tttaattatt gctgatgaag gatccgaagt acactatatt
    11881 gaaggctgta ctgctccggt ttatacaact gaatccttac actctgctgt tgttgaatta
    11941 attgctaaga aaaatgcaaa aataagatac actaccattc agaattggag taaagatgtt
    12001 tataacttag taacaaaaag agcatatgcc tatgaaaatg ctcgagttga atggattgac
    12061 ggaaacattg gaagcaaatt gactatgaaa tatccaggag tttatatgct tggaagaaat
    12121 gctcatgctg aagttgtatc gattgccttt gctggatcgg atcaacatca agatgcagga
    12181 gcaaaggctg ttcatttagc tcctgatacc acgtctagaa ttacaagcaa gtctgtcagt
    12241 aaacaatccg gcagaacaac atatcgaggt ctattgcatg tagcaaaagg tgctactggc
    12301 gttaaatcaa acgttcgatg tgatgctctt ttacttgatg aaacttcacg aacagataca
    12361 tatccgtatg tcgaagttaa tgaagatgat gcgacaatct ctcatgaggc tagtgtggga
    12421 aaaattggtg aagatcaaat tttctatctt atgagtaggg ggttctcaga gtctgatgcc
    12481 ttgtcactta tagtgggcgg atttatagaa ccatttacaa aagaattacc catggaatat
    12541 gcagtagagt taaatcgact tgtcaaacta gaaatggaag actctgtagg ttagcaaata
    12601 cttatctttt tttgaacgtg atattcataa catgtaccaa ctatctttgc tagaaaaaaa
    12661 ttttgatagt tatattgaaa actttaaaga accactatgg atgaaacaaa ctcgatatga
    12721 ggcctttaaa aattatgcca gcctaccctt agaatcctct tcactttata gtaaatattc
    12781 aaatgcaaat aaattaattg ctgataaagt ttttttaaat gatactaatt tagaaactaa
    12841 atcctcctcc aatgatttag atattttttc tgatagaata gatgagttta gtaaaactac
    12901 tagtgtttta tgtaaaaatt caaaaattct tgaatcattt gtcacagatg agctggaaaa
    12961 aaaaggcata attattttaa atatttctca agctctaagt gattattcag atctaataca
    13021 aaaatatctt aacaaaaaca aattaaatta tctagaggac aaatttttgg ctctctcttc
    13081 agcagcattt caaaatggac tttttatttt tatccctaag aatattgtat tagaagatcc
    13141 tatcagaata gtaaattatc tttcagagga tggtctatca tcaatatcta gaaatatagt
    13201 agtatgtgaa gaattttcga aagctacttt agttcaagaa ttatattcac ccaaattaag
    13261 tagttcaaaa tatcaacaaa cacatttcga attactagag tcccacatca tgcctaatgc
    13321 tcaatgtgag atagttacct tacaatcgat ggactatgat gcaattagtt tttcgaatag
    13381 aaaagcctat gtagaaagag atggaaagat gtcatggtct ttagctcttt ttgggtcaca
    13441 attgtcaagg tataaagttg atagcattat gcaagggcaa ggagctttag ctgaagatgt
    13501 tgaaattatt tttggaatag gtaatcaatc ctttgacatt acttccaatt tgattcatgt
    13561 agctcctcat acccgtggta aagttttagt gaaatctgta atgaaggata cttccaagtc
    13621 tctttttaaa ggaatgataa aaattcataa aaatgctaaa gctactgagt cttatttagc
    13681 aggtcatgct atattattgg ataaaggtgc aaaatctgat gcaattccag gcttggaaat
    13741 agagtctaac gaagttaagg cgacacattc tgcctcggtc gctcagatag atgaaaagca
    13801 aatcttctac cttatgtcac gtggtctttc agaagagtta gcgaaaagag agatagttgg
    13861 tggtttttta gaacctatct caagaaaaat gggcccaact attcgtgcct ggattaatta
    13921 tttaatcgaa aataaatggt taggcaaacc gttaaagttg aaaatagatg aagcaatgga
    13981 acaaattcta gagattgaaa aaagtagata tagagaaacc gaagatattt ttgaaaaaca
    14041 ctacaagtat aggtaacaaa tattaacctt aatttttttg tagataaata atgtcaaatt
    14101 gggtaagagt ttgtaatatt gattctctta aacctggaga attgatggat tttgatttta
    14161 atgacaagaa aattcttctt gttaatataa agaataaaat atatgctagt gatagaattt
    14221 gcactcatgc ttatgctgat ctatctatgg gttttttaaa tgaagaagaa aaaactgtta
    14281 catgtcctct tcatatgtct gcatttaatc ttaaagatgg attaccactt aacttaccag
    14341 cagaagaacc attaacggtt tatgaaacta agatagataa caattcagtt tttatatttg
    14401 tctaatgtaa aatacacaca aaggtgtatt agtgcacaaa tttaaatggg ttaataaaat
    14461 attaaaataa tatttatgca aattaatcaa actgataaag caagttttta aatcctttga
    14521 aatcttaaac aaatactatc atgaatattc aaaaccgtac ttatttgcaa ttttttattg
    14581 ctctttattg taattgaaaa gatttattat tatgttttaa tgaaaataaa aatatttttt
    14641 tatccttata attaatgtta ttctattgaa aattggaatt attggattgg gattaatggg
    14701 tagtaatctt tgcttacgtc taattagaaa aggatttcaa gtgaatgtta ataatcgcga
    14761 tctttcaaaa tcgctattat taaaaaaaaa aggcgctaaa ctttttaata caccaaaaga
    14821 tgtagcagat tattccgatt ttatcataac ttgtgtaaca aattttgaag cagttaatag
    14881 aatatttttt catgaaaatg gcgtaagtga aacagaaaac aaaaatgtaa ttgtttcaga
    14941 cttcagtact gtttctccaa aagaatcagt ttattgttat caaaaattta aagaaaataa
    15001 tttatcaatg ttaagtattc cagtaatggg tggccctaat gcagcattac atgggaaact
    15061 cataccgatt gtatcaggag aagaaacatc atttaagaaa ataaaaaatg tcttagaaga
    15121 attaggtgat cctattttct atattggaaa taagctgggc tccgccaatg ctatcaaatt
    15181 ggcgcttaat ctaaatattg gtataatagc aatggcttta tcagaaggat tattattagc
    15241 tgacaaatat gaaattgatc ctaa
//
LOCUS       DASVEV010000148         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1219408, whole genome shotgun sequence.
ACCESSION   DASVEV010000148 DASVEV010000000
VERSION     DASVEV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219408"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..863
                     /locus_tag="VF222_06845"
     CDS             141..863
                     /locus_tag="VF222_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142178.1"
                     /translation="MSRLYYSERSSVSILVKIGFVLGINLLLLNQIVQILAQDEKINV
                     EPINMTNHITENLQDLKISFDSIKINNDHDLLYEGEWKIDAYVNGKKISLLSNSSMRV
                     ESGQTVVFPKGDKFMNIQIMDNQTLRIVTLGIEYDSKTDQVKNYLPDISSILDNQNVP
                     LSEDKDKSQFGMEPFTAFDRNDAIGIVAKEFTAKNNFGIGLNHDYCSESSGEEGDLYD
                     IVDTNCDFQLSFSIEANNKIEK"
     gene            864..1769
                     /locus_tag="VF222_06850"
     CDS             864..1769
                     /locus_tag="VF222_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX7142179.1"
                     /translation="MTYMYLSLSLLFILIALLCITLYDKEETITTTEWKLVPYVEAHT
                     GIEIKPDENGVLTVNYHGVQGKVYNPLTVASGALKYYSDYVKEIDKEQSINKFISSAD
                     WLVNNAKDKGVYSLWEYDYPWMFYGWVSPPYSSALAQAKGLQVLTLAYELTGNEKYLD
                     LAKKTIRSFLVDYEEGGFVTHESVGNNSLFLHILAKPGFPKTYVLNGHTNSLLSIWSY
                     YEKTQDPIAKTIFDKGINYLKGNLVKYDTGSWSYYDLMENWAMTDYHKGQIQQFKVLY
                     QITREPILRDYADRFEKYLKYSSVE"
     gene            complement(1962..3143)
                     /locus_tag="VF222_06855"
     CDS             complement(1962..3143)
                     /locus_tag="VF222_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7142180.1"
                     /translation="MPSDEIENVLILQGGGSLGAFACGVFKTFVENNIKIDIVAGTSI
                     GAVNASIISGSKQKDNPEKALEQFWLELAEGDVNSNSSSFTKWLLYGITNPLTSIEDH
                     ARNIPSSQIKSLSSFYKSAFFGNNKVFIPRWSPQYAFTDPEYFTPNKWTYLYDHSPLI
                     KTLEKYIDFSKLQPNGNPNSRLIITAVNVLTAEPLAFDSSSQQITPKHILAATAYPKY
                     YFPWVEVEKGVYAWDGSLLSNTPLREVIDASPVKNKLVFLVENYPKNIDYLPANLFEV
                     EHRTRDILFSDKTMHNIKMSKAFTVYLKLMDDLYNMLENNFNSQNKEDIKKFERIRKR
                     YKKISENHGAEIKKTYYISREEKEPHLYENADFSVDVIKSSIKDGERQTNQVINEMSK
                     K"
BASE COUNT         1296 a          511 c          587 g         1287 t
ORIGIN      
        1 aaatatgtag attttggact ttcgagagct aatgcttaaa ttgatgtttg taaggcaata
       61 atttaaattg attttgcata gaagtagacg aatattctat aatgaaagaa tttgatagta
      121 cagttaagag atttaatttt atgagcaggt tatattattc cgaaagaagt tctgtatcca
      181 tcttggttaa gattggtttt gtccttggta tcaacttgct gttattaaac caaattgtac
      241 agattctagc tcaagatgaa aagataaatg ttgaaccgat caatatgact aaccacatca
      301 cagaaaatct acaggatttg aaaatatcct tcgattcgat caagataaac aatgaccatg
      361 atttattata tgaaggggaa tggaagattg atgcttatgt aaatggaaaa aaaatatcct
      421 tgctctcaaa ttcaagtatg cgagtcgaaa gtggtcagac agttgtattt ccgaaaggag
      481 ataagtttat gaatatacaa ataatggata atcaaacttt gagaattgta actttaggta
      541 tagaatatga cagtaaaaca gaccaagtaa aaaattactt accagatatt tcaagcatat
      601 tagataatca gaatgttcca ttatcagaag ataaagacaa gtcacagttt ggtatggaac
      661 catttacagc ttttgatagg aatgatgcta taggtatagt agctaaagaa tttactgcta
      721 agaacaattt cggaatcggc ttaaatcatg actattgttc tgaaagttcc ggggaagagg
      781 gcgatctata cgatattgtc gacactaatt gtgattttca attgagtttt tctatagaag
      841 cgaataataa aattgagaaa taaatgactt atatgtatct ctctttatct ctgcttttta
      901 ttttaatagc cctgctttgt attacacttt atgataagga agagacaatc acaaccacag
      961 aatggaaatt agttccttat gtagaggctc ataccggaat tgagataaaa cctgatgaaa
     1021 atggggtgtt aacggttaat tatcatggcg ttcaagggaa agtatataat cctttaactg
     1081 ttgcaagtgg agcgttaaag tattatagcg actatgtaaa agagatagat aaagaacagt
     1141 caataaataa atttatcagt tcagctgact ggctagtaaa caatgctaag gataaaggag
     1201 tatattcttt atgggaatat gattatccat ggatgtttta tggttgggta tcacccccat
     1261 attcgtctgc tttagcccaa gcaaaaggac ttcaagtatt aacgttagct tacgaattaa
     1321 caggaaatga aaaatatctt gatctagcaa aaaaaacaat cagatctttt ttggtcgatt
     1381 acgaggaagg aggctttgta acccacgaaa gtgttgggaa taactctttg tttcttcata
     1441 ttttggcaaa acctggcttt ccaaagacat atgttttaaa tggtcatact aattctctat
     1501 tgtctatttg gtcttattat gaaaaaaccc aagatcccat agcaaaaaca atatttgaca
     1561 aaggaataaa ttatttaaaa ggaaatctgg tgaaatatga tactggcagt tggtcgtatt
     1621 atgacctaat ggaaaattgg gcaatgacag attatcacaa agggcaaatt cagcaattca
     1681 aagtgcttta tcaaattact agagaaccga tattgagaga ttatgcagat agatttgaaa
     1741 aatatctaaa atattcttca gtagaataat caatacctgt tagtatttta tagatataat
     1801 aatatttgaa catacaataa taataataat tcctttgttg gttttgttgg atattccagt
     1861 atgaccagaa acaactttaa aaataatgta tatttagtta tgtataataa tatataaaat
     1921 taataacaat aaattcatga tacaaaatta tacttttgta actatttttt agacatttca
     1981 ttaattactt gatttgtttg tcgttctccg tcttttattg aactctttat gacatctact
     2041 gaaaaatcag cattttcata aagatgaggc tctttttctt ctctcgatat ataatatgtt
     2101 ttcttaattt ctgcaccatg gttttctgat atttttttat accttttacg aatcctttca
     2161 aatttcttta tatcttcttt gttttgtgaa ttaaaattat tttcaagcat gttataaaga
     2221 tcatccatta gcttcaaata gactgtaaaa gcttttgaca tttttatatt atgcattgtc
     2281 ttgtcactaa acaaaatgtc acgcgttctg tgttccacct caaacaaatt cgcaggtaaa
     2341 taatcaatat tttttggata attttcaact agaaagacca gtttattctt tacgggtgaa
     2401 gcgtcaatga cttctcttag tggggtatta ctaagcaaac tgccatccca tgcgtagaca
     2461 cctttctcta cttctaccca tggaaaatag tatttaggat aagcagttgc agcaagtata
     2521 tgttttggag ttatttgttg actagaacta tcaaatgcta atggttctgc tgttaagaca
     2581 ttaactgcag ttattattaa acggctatta ggattaccat tgggttgtag tttgctaaaa
     2641 tcaatgtatt tttctaatgt ttttattagt ggagaatgat cgtataaata tgtccattta
     2701 ttaggagtaa aatattctgg atcggtgaaa gcatactgag gactccagcg tgggataaag
     2761 accttattat ttccaaaaaa tgctgattta tagaatgatg acagagattt aatctgtgaa
     2821 ctaggaatat ttcttgcatg atcttctatc gaagttagtg gattggttat tccatataat
     2881 aaccacttcg taaaagagga tgagttggag tttacatcac cctctgctag ttctaaccag
     2941 aattgttcta atgccttttc aggattatct ttctgtttac ttccagatat tattgaagca
     3001 tttactgcac caattgatgt accagcaact atatctattt ttatattatt ttctacaaat
     3061 gttttaaaaa caccacatgc aaaagcacct aaagaaccac caccttgaag tattagaaca
     3121 ttttcaatct catctgacgg catacaaaaa taacattcgc tataataatt tatatctaat
     3181 tgggctctta atttaagtag taaaagctat atctaacaat ccaaaatata ttgcaaatat
     3241 acatttaggc aatatacgta tgttaaatgg acaagtttta caaaataata agccgattga
     3301 tgtaatttac tataatacaa aagtagaaaa ataaatttcg tattactctt tctaaatatt
     3361 taatatttac tacctatgca taaaattata ggaaatctgt ttatctaata tcatatactt
     3421 ttaatatcca taaaaaacaa agaaaagaaa atattctata gaaataattg tttgtatatt
     3481 ttacttctct caggttttga tcttattctc tacaatttta tattttgcga gtatttatag
     3541 caataaattg aactagtatt aattaactaa taatgaatca tattcatatg aaataaatat
     3601 ttccaaggtc taatgtatta tgatattagt tacgatttat tttggtagat atataaacca
     3661 gcgaatatag ttgcagggtt c
//
LOCUS       DASVEV010000149         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1025539, whole genome shotgun sequence.
ACCESSION   DASVEV010000149 DASVEV010000000
VERSION     DASVEV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025539"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF222_06860"
     CDS             <1..190
                     /locus_tag="VF222_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142181.1"
                     /translation="ICSGHKAIRPSNGQRYLIGQKRCQVCQIFINWEGVFCPCCGYKL
                     RVTPRNRKLKLTLRNLKN"
     gene            401..631
                     /locus_tag="VF222_06865"
     CDS             401..631
                     /locus_tag="VF222_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142182.1"
                     /translation="MVKKKKEIKIIRKDSNKEDYTFVSSDNILNNVKDIESAITTHNE
                     KKSDNKFDERFEKKVHSKKVDNKGRKILGKSM"
     gene            complement(876..1178)
                     /locus_tag="VF222_06870"
     CDS             complement(876..1178)
                     /locus_tag="VF222_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142183.1"
                     /translation="MTVIALLANEFGYQLEIGDHTGKFIQDAEIKILHNRTSIKKKIL
                     NNTLITDDGKIVNAIGLISTVADSKVIGGTEHINIYFFYFISLNKYMYYLKTNNLI"
     gene            complement(1241..1495)
                     /locus_tag="VF222_06875"
     CDS             complement(1241..1495)
                     /locus_tag="VF222_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142184.1"
                     /translation="MYKKRLNINKLNILLLKVILDKTIDEVYLKRFNISLDKIIHSIE
                     NPDSKKIIKLQDYKVIYVLKKFDNYYLLIDGRSETGDEVK"
     gene            complement(1554..1700)
                     /locus_tag="VF222_06880"
     CDS             complement(1554..1700)
                     /locus_tag="VF222_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142185.1"
                     /translation="MTFSDSQISKEDKGWLYPQMIQSIKKIAADSIILVFSPIKLAGL
                     INYK"
     gene            complement(1765..1932)
                     /locus_tag="VF222_06885"
     CDS             complement(1765..1932)
                     /locus_tag="VF222_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142186.1"
                     /translation="MAIANPDIALAVNQLAYPPDYDPDIDYTDKECAKMFPNFSEGYY
                     DCTGGIPNPGP"
     gene            2218..2409
                     /locus_tag="VF222_06890"
     CDS             2218..2409
                     /locus_tag="VF222_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142187.1"
                     /translation="MIVWALISIIIITSFPFQISSVLFENNRHLDIKNIGQEVYAEDD
                     DEDDEDGSMIKNLMTTTYP"
     gene            2843..3034
                     /locus_tag="VF222_06895"
     CDS             2843..3034
                     /locus_tag="VF222_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142188.1"
                     /translation="MGVETDEKEDTKGRYKFPYSDFAKVHRCGVLAAENRAGQYKYND
                     IETAAAHLHGMIDAARNDV"
     gene            3744..4028
                     /locus_tag="VF222_06900"
     CDS             3744..4028
                     /locus_tag="VF222_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142189.1"
                     /translation="MNQKNSFNTSKKFNKHHHSEEFLLYKSINEKEALCPSCNHSLGR
                     HKIRIPHHCTEKLCNCKLSRLEAKTDYFKWFMKERRNQKNKESFLNFNDA"
     gene            4184..4642
                     /locus_tag="VF222_06905"
     CDS             4184..4642
                     /locus_tag="VF222_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142190.1"
                     /translation="MVEASIAEYFSIGGALGIIGSMFVVLYFSRKQMQILSKDIETKI
                     LNDMDESLRGITQIGVERPELIKVISNIPANYCSPEVSFAYYILYTYAHVFHMWKRGV
                     VNDNEWTGWLRYMKSAFEQGTIAETWKTIDARKWFDPDFEEFINKELAKK"
     gene            complement(4930..5448)
                     /locus_tag="VF222_06910"
     CDS             complement(4930..5448)
                     /locus_tag="VF222_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142191.1"
                     /translation="MSKRIENIKKNPKTKNMFVSGLFLPITIVFMVFVNIASICYVQV
                     YSQEQEQNQTQTNQTFIELTGKLIGTTYRWIDSNNTINPTLIINSGVNNQISVKSLKD
                     DSQEHDFIIEGITSSGDKEELVISDTVHDGSSSIVDFYPLDPHEYKNYKSFDYYCEFY
                     PETMRGNIKITN"
     gene            6515..>7074
                     /locus_tag="VF222_06915"
     CDS             6515..>7074
                     /locus_tag="VF222_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142192.1"
                     /translation="MNKTLIIYILATLIAFSSVTTITMQPIDISAQTQPAAPTQSPLQ
                     TQQQPSTQPLAISPSCGQVITQNVVLTSNLNCADSDGLIVGASDIVVDLNGHTISGPD
                     VDSDTKTSSKVGVMIPNMNNVVVHDGTIEGFQAGILMTGSQNVEVKGVVAKNNQIGLF
                     STGASILNAHLSIIMNNQIGIAGHST"
BASE COUNT         2684 a          970 c         1049 g         2371 t
ORIGIN      
        1 aatatgttcg ggccataaag caattaggcc ctcaaatggt cagcgttatt tgataggaca
       61 aaaacgctgt caagtttgtc aaattttcat caattgggaa ggtgtatttt gcccttgttg
      121 tggttataaa ttaagggtta cacccagaaa caggaaatta aaactaacat taagaaatct
      181 taaaaattag attttacttt gttagttagt ttaaaaaact ttatatttaa tatcttgata
      241 caaaaaatat tgcattttat caatccttga tataacatgc tttccattat atcacattta
      301 tcaaagtaat tagcaacatt cactaattca tttattcatc atttattttg caataacatt
      361 aattgataac ttgcaaatat ataatagata ggttaacaaa atggttaaga aaaaaaaaga
      421 aattaagata ataagaaaag attctaacaa agaagattat acttttgtta gcagcgataa
      481 cattctaaat aatgtaaaag atattgaatc tgcaataaca acacataatg aaaaaaagtc
      541 agataataaa tttgatgaga gatttgagaa aaaggttcac tcaaagaaag tagataacaa
      601 aggaagaaag attttaggaa aaagtatgta aggtcatcac cagtatatga ataatcatat
      661 tgtagaatat aagttaccaa aaagctaaat actatgtaat agaaccctac tacatggacg
      721 attccactct caaatcactt acaggtttct tggacacgct atctccgtca agataatata
      781 ccctcaagtg taacaggttc gaactgcata aataaggttt tatagtgtaa ttgaatccaa
      841 gaaaaataat cttattatta atttgaaagg taatatcata tcaaattatt tgtctttaga
      901 taatacatgt atttatttag gcttataaaa tagaagaaat agatatttat atgttcagtt
      961 cctccaataa ctttagaatc tgcaactgta gaaattaaac ctattgcatt gactatcttt
     1021 ccatcatctg ttatcaaggt attatttaat atcttctttt ttatgctagt acgattatgt
     1081 agaattttta tttctgcatc ttggataaat ttacctgtat ggtcgccaat ttctagctga
     1141 taaccaaact cattagctaa tagagcaata actgtcaatg gattttcaat tgatatattt
     1201 tgtactagtt gtttgccaac tatgaataca ctatttacct ttactttact tcatctccag
     1261 tttcacttct accatctatc aaaagatagt aattgtcaaa tttttttaga acgtatatta
     1321 ctttataatc ttgaagcttg attattttct tgctatctgg attctctatt gaatgtatta
     1381 ttttatctag agatatatta aaacgcttta aatatacttc atctatagtt ttatctaata
     1441 tgaccttcaa cagtaatata tttaatttgt tgatatttag ccgtttttta tacattcagt
     1501 aaaatataat taaatatctt ctttttgcta gttgtagctt actttaatat aatctattta
     1561 taatttatta aacctgccaa ctttataggg gaaaagacaa gaataataga atctgcagca
     1621 atctttttga ttgattgtat catttgagga tataaccagc ctttatcttc ttttgaaatt
     1681 tgggaatctg agaaagtcat ctcatcaatg gttacctttc taaatagaat atttttcaat
     1741 gaatatggat aaagaaaaaa atagttaagg acctggatta ggtattccgc cagtacagtc
     1801 ataatatcct tcactgaaat taggaaacat tttagcacat tccttgtctg tatagtctat
     1861 gtctgggtca taatctggag gataagcgag ctggttcact gctaacgcta tatctggatt
     1921 tgctattgcc atcattagta caattacaga tattatggag gttatggaag tggttaatat
     1981 tttgttcaat atataattat attaagaatg gttacatata aggacagtgc tttcttatta
     2041 tttttattat ataaatagtt ggagaccgtt gatcaaaata ttaatgcata gtattaaaga
     2101 taaatgtaaa taacatgatt agaaaactag aaaatagaaa cttaaaataa caaatctttt
     2161 atagtcaaga aatataaaac aatcattttt atgaaaataa aaaagtcagc attcgggttg
     2221 attgtatggg cattaattag cataattata atcacaagtt ttccttttca aataagcagt
     2281 gttttatttg aaaacaacag acatttagat ataaaaaata ttggccaaga agtttatgct
     2341 gaagatgatg atgaggatga cgaagacggt agtatgataa agaatctgat gacaacgacg
     2401 tacccttaga agaaccaata aatctactct aggaggatga actagaaaga gaagatgtag
     2461 acgagtactg tgatgcttcc gaagattata ataaacatcc aaaacagtgt gagaaactct
     2521 ataatatagt agaaaaattt gaagaaaaac atcaggaaac aacaacaaat gaagattaga
     2581 gtagtgaagt acagggacaa gaagaagagc tatctctact attcaaacat ttatcattga
     2641 tgcagaaata attaaaatta aaggaggtga ccatctgaca ataaagctga ataaacgggc
     2701 atttgagcat gcgaaacgac taatagaaaa aggaagctat gtgtttgatg aaaaagatat
     2761 ctggagcgag cataaaccgt ctgcacaaga agagaatgag tatattaaaa agtataattt
     2821 tgatgaatat gggaagtggt gtttgggagt agagacagat gaaaaagaag atactaaagg
     2881 acgatataaa ttcccttata gcgattttgc caaagtacat cgatgtgggg tgctggctgc
     2941 agaaaacaga gcgggacaat ataagtataa tgatattgaa acggcagcag cacatttaca
     3001 tggcatgatt gatgcagcaa gaaacgatgt ttagtagtaa gagtaaacct atataatatc
     3061 cgctatggat aaatatatcg tagtgattta tccttaccta aaccttttct acagaaatca
     3121 attcctcagg tatatcttta tcagattatc ctctaaacct ttttgaattt agtatcttat
     3181 aacttatcaa aatttagaac ttaataatat tttctaatct ttggtttcca gtaaacacta
     3241 atacgtacct attataataa acaaattgtt atctataata acgtgattaa actagaatgg
     3301 aggtaaacaa taatattgtg aacatcttca ctataatatt gaataaaaaa cttagtaaat
     3361 caattttaga tacctattca ctaattccct tcacaaaata ttttaccata gttcgtatat
     3421 ttaaaaattt tttcattttt tattaatata tgcacaattt gttggtaata agaaaaattg
     3481 ctaacactag agaatctatt gatataataa tactcagcaa aattttttcc ttatctttac
     3541 aactacaatc aagtggaaac tttaaagagt agtattgttg taactggtat atatcaattt
     3601 tatgaagtag tttagtaaat tgaacagatt tcaaatctat acgttgttta gaataatcat
     3661 tctttcctcc atctggttgg aaaacagaat gtatccaaat actgtatcag tatatatact
     3721 agaaaatcag ataggaacaa aaaatgaacc aaaagaattc attcaataca tctaaaaagt
     3781 tcaataaaca tcatcatagt gaagaattct tactttacaa aagcataaac gaaaaggaag
     3841 cactatgtcc tagttgcaac cattcattag gcagacacaa gattagaatt cctcatcact
     3901 gtactgagaa attatgtaat tgtaaacttt caagacttga ggcaaaaacg gattatttca
     3961 agtggtttat gaaagaaaga agaaatcaaa aaaataaaga atcgtttctc aattttaatg
     4021 atgcttaatt gttagggata tggataaaaa aagctaaata tgaaattgta ctatatctgc
     4081 ttcttctttt tttatctaac tgcatttcca actggtagga tttagattat acatctgaaa
     4141 agaaactctt atcaagatat cttttattta taagtaacaa gtaatggttg aagctagtat
     4201 agctgaatat ttcagcatcg gaggagcact tggaattata ggtagtatgt ttgtagttct
     4261 atatttttca agaaaacaaa tgcagatcct atccaaagat atagaaacaa agattctcaa
     4321 tgatatggat gaaagtttgc gtggaattac tcagattgga gtagaacggc cagaactgat
     4381 taaagtaata agcaatattc cagcaaacta ttgttcacca gaggtatcat ttgcttatta
     4441 tattctatac acatatgccc atgtattcca tatgtggaaa agaggagtag taaacgataa
     4501 cgaatggaca ggatggttgc gatatatgaa aagcgctttt gaacaaggta ctatagcaga
     4561 aacatggaaa actatagatg caagaaaatg gtttgatcct gactttgagg aattcattaa
     4621 taaagaatta gctaaaaaat aatgaaaaga agaattatct ctattagtct ttttgcaatc
     4681 tttatattcc caagactatc acttatatag tcaaattcca gttccacttt tattgtctta
     4741 taagatgggc caaaaatatt gtgtcaataa caattcacac acctctgagc ccaatgaata
     4801 tctatatgaa atcagataac tggaatatta tcttcattta gaactcccca cccatttttt
     4861 agaatacaat gacgatccca attgttaaaa tgtgactcta aatttcagaa ataaataata
     4921 caatagattt taatttgtta tttttatatt tcctctcatt gtctctggat agaattcaca
     4981 ataataatca aatgacttat aattcttata ttcgtgtgga tctaaaggat aaaaatcaac
     5041 tatactcgac gacccatcgt gaactgtatc acttataact aattcctctt tatcaccact
     5101 agaagtaata ccttctatta taaagtcatg ttcttgagaa tcatctttca aacttttgac
     5161 actaatttga ttatttactc ctgaattaat tattaaagta ggattaatag tattgttaga
     5221 gtctatccat ctataggtag taccaattaa tttacctgtc aattcgataa aagtttgatt
     5281 tgtttgtgtt tgattttgtt cttgttcttg agaataaacc tgtacataac atattgaggc
     5341 aatatttaca aataccatga aaactattgt aattggtaaa aataaacctg aaacaaacat
     5401 gttttttgtt ttaggatttt ttttaatatt ttctatcctt tttgacatta ttttagaata
     5461 ctaaagatag tttattatgg ttatggtaat tcaaaaaata ttttatactt tatctttttt
     5521 tagatatgaa tactattggg ttgaattact aagacgttca taatttcaat aaataaatat
     5581 aataaagaca ttagaataaa acttggatta cagacaaaaa tgaccgtcaa acctctgagc
     5641 cccttctata agtgggttca tatactttga atttgatatt agattttata aagaaggaaa
     5701 ggaggataat aaaggaaaga aaatgccatt gaaactatct aaaacaatag gtaaaattca
     5761 acacattccc aattccaaga aataaagaca ataaatgaat ttctagaatt atgagaagta
     5821 agggttcttc agaacatcat caaaataata atttaaaagt tataataaca tttagtaatt
     5881 tcataagatc ctccttttat ggcattaata aaagaaaaca aattttagaa tttttaaatc
     5941 aaaaagttaa aagataagat gatcatgaca agtgatggat ggatggataa ctaattagaa
     6001 caattactca atcgtttaag attatttttt aaatggttat acaatcatga tatgaaattg
     6061 accatgacaa atggtacact cgaatctgtt agagaataaa aaagcaaacg gatatcaata
     6121 tatttgattc cttattctca ttgttaaaga atctttattt atcagtagtc tgtattatta
     6181 ttattacatg aactactatc aattctctat aatgatatca attttagata ggcatttatg
     6241 ccattatatg cctaatgtgt tctttcattg ttattattaa ttgtcattat agagaatgat
     6301 agtaatagta atagcagtat tagtacatga attttttata atgcaacaag ttgaaattga
     6361 aatccttttt ctatgagtaa tgtatttttt aagaatggaa tggaatatgc cattaaatta
     6421 aagttattta aagtgattac cttcatagtt acagtaaaat tgataaataa aattattagt
     6481 ttagcataaa tattttattt ttaattaatc tgtaatgaat aaaacactta taatatatat
     6541 cctagcaaca ctaattgcat tttcaagcgt tacaacaata acaatgcaac ctatagacat
     6601 aagcgctcaa acccaaccag cagcacccac acaatcacca ctacaaacac aacaacaacc
     6661 atcaacacaa ccattagcca taagtccatc atgcggtcaa gttatcacac aaaatgttgt
     6721 attaacttca aacttaaact gtgcagatag tgatggtctt atagttggtg cgagtgatat
     6781 tgttgtagat cttaacggac atacaatatc tggtccagat gtagatagcg atacaaagac
     6841 ttctagtaag gtaggagtca tgattcctaa catgaacaat gttgtagttc atgatggtac
     6901 tattgaagga tttcaagctg gtatactaat gacaggatct caaaatgttg aagtaaaagg
     6961 agtagttgct aaaaataacc aaataggatt attcagtacc ggtgctagca tactcaatgc
     7021 acatttgtcc ataataatga ataaccaaat tggtattgca ggacactcta caca
//
LOCUS       DASVEV010000150         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1609734, whole genome shotgun sequence.
ACCESSION   DASVEV010000150 DASVEV010000000
VERSION     DASVEV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609734"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..330)
                     /locus_tag="VF222_06920"
     CDS             complement(16..330)
                     /locus_tag="VF222_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142193.1"
                     /translation="MSSSSFWRDFPVHKTLLLEKLEQVNSVCDSMILDYMRLVQTNDF
                     SYRILLPRNEENNKLAKTIGLSLQLEFTSQLRQRKIKTRMREIRYIHDKNHFGWIIIN
                     SD"
     gene            complement(381..809)
                     /locus_tag="VF222_06925"
     CDS             complement(381..809)
                     /locus_tag="VF222_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX7142194.1"
                     /translation="MSDDIYREIILDHYRNPRNKGKIENADVVIHDSNPLCGDQIDMY
                     LKVVEGQIKDIKFDGKGCAISQASASMLTEMVMDKPLTTAKDISKDEVLENIGLTNLG
                     PARIKCALLSLKVLKMGMVKYYSDKEPTTAEKMENDSNLF"
     gene            complement(806..2050)
                     /locus_tag="VF222_06930"
     CDS             complement(806..2050)
                     /locus_tag="VF222_06930"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX7142195.1"
                     /translation="MQLSELETNKIRNDFPILNRKINGKPFVYLDNASTTQKPLSVIN
                     KIVNYYSFYNSNIHRAVYDIAEEATNAYELTRKKVCKFINANSTQEIIFTRNATEAIN
                     LVSYSWGRKNIKTGDLIILSEQEHHSNIVPWQILAKEKNAEIKYLKIDERGNLNLNEL
                     EFYLNNNNNVKLISLCHMSNALGNIIPIDKIIDLAHAYGIPVFVDGAQSVPHLPTDVT
                     TLDCDFLAFSAHKMLGPTGVGILYGKKNILEEMDPFICGGDMIKEVHKENTFFNDLPY
                     KFEAGTPNIADVIGFSSALDYLNEIGMDNIRNHEIELTKYALEKMSELKQIQLYGDIN
                     IQNRGGIISFNLGDIHPHDLATILNEHCIAIRSGHHCAQVLMEKLEISATSRISFYIY
                     NTKEEINIFINALGKAIKLFKL"
     gene            complement(2141..>2377)
                     /locus_tag="VF222_06935"
     CDS             complement(2141..>2377)
                     /locus_tag="VF222_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HEX7142196.1"
                     /translation="GVYSDPTKDPRGHMLTVVFIVLVIRGNPSTGDDINEISWMPINK
                     INDLDIAFDHKLIINDYLKWKKEGGTYWTSKKRY"
BASE COUNT          753 a          388 c          342 g          894 t
ORIGIN      
        1 atatgcattt tttgttcagt cagaatttat tataatccat ccaaaatgat ttttatcatg
       61 tatatatcta atttctctca tcctagtttt tatttttcgt tgtctcaatt gagaagtaaa
      121 ttctaattga agactaaggc caattgtttt ggctaatttg ttattttctt catttcttgg
      181 taatagaatt ctataactaa aatcatttgt ttgtaccaat ctcatgtaat caagaatcat
      241 gctatcacaa acagaattaa cttgttctaa tttttctagt agtagagttt tatgtaccgg
      301 gaaatctctc caaaatgaag aacttgacat ataggggtta gtagatttta aagaaaaaat
      361 attatttaaa taaaactaag ttagaaaagg tttgaatcgt tttccatttt ttcagcagta
      421 gttggttctt tgtcagaata gtattttacc atacccatct ttaagacctt gagagatagt
      481 aatgcacatt ttatccttgc agggcctaag ttagttaatc ctatattttc taacacttca
      541 tctttggata tatctttagc ggtagtcaaa ggcttatcca taaccatctc agtaagcatc
      601 gatgcactag cttgactgat agcacaccct tttccatcga atttaatatc cttgatttgt
      661 ccttctacta ctttgagata catatcaatt tgatcaccac ataagggatt gctatcatgg
      721 atgacaacat ctgcattctc aatcttaccc ttatttctag gatttctata atgatctaga
      781 ataatttctc tatatatatc atcactcaaa gtttaaacag cttaatagct ttgcctaacg
      841 cattaataaa gatattgatc tcctctttcg tattataaat atagaaacta atcctagaag
      901 tagcagagat ctcaagcttt tccattaaca cttgggcaca gtggtgaccg gaacgaattg
      961 caatacagtg ctcatttaaa atagttgcta aatcatgtgg atggatatca cctaaattaa
     1021 aggatataat tcctccacga ttttgtatgt taatatctcc atatagttgt atttgtttaa
     1081 gttcagacat tttttcaagt gcatattttg ttaattcaat ttcatggttt ctgatattgt
     1141 ccatccctat ttcgtttaga taatctaaag cagaactaaa accaataaca tcggctatat
     1201 taggtgtccc agcttcaaat ttataaggta gatcgttaaa gaatgtgttt tctttatgaa
     1261 cttctttaat catatcaccc ccacagataa acgggtccat ttcttctagg atattttttt
     1321 tcccatacaa aatcccgact ccggtgggtc ctaacatttt atgagcggag aaagcaagaa
     1381 agtcacaatc aagagtagta acatccgttg gtaaatgagg aacagactga gcaccatcaa
     1441 caaatacagg aataccatat gcatgtgcaa ggtctatgat tttgtctatt ggtataatat
     1501 tgccaagtgc attagacatg tgacataaag aaattaattt tacattatta ttgttgttaa
     1561 ggtaaaattc taattcattt aaatttaagt tacccctttc atcaattttt aaatatttta
     1621 tttccgcatt tttttcttta gccaaaattt gccaaggaac aatattgcta tgatgttctt
     1681 gctccgataa aattatcaga tcccctgttt tgatattttt tctaccccaa gagtatgaga
     1741 ctaaatttat tgcttcagta gcattgcgag taaaaattat ttcctgagta gaattggcat
     1801 taataaattt gcatactttt tttcttgtta gttcataagc attagtagct tcttctgcta
     1861 tatcataaac agctctatga atattagaat tataaaacga ataataattt acaattttat
     1921 taataactga taagggtttt tgagtggttg atgcattgtc gagatagacg aatggtttac
     1981 cattaatttt gcgatttaga attggaaaat cattacgtat tttatttgtt tccaactcag
     2041 ataattgcat aatttattat tattatgtag taaatataaa tggatggaag caaaagaaat
     2101 atttccagat ttcttatttt aaagccattt ataacatata ttaatacctt ttcttgctag
     2161 tccaataggt tcctccttct ttcttccatt tcaaataatc atttattatt agtttatgat
     2221 caaatgcgat gtctagatca tttattttat ttattggcat ccaggaaatt tcattgatgt
     2281 cgtcccctgt agagggattt cctctaataa ctaatacaat aaatacaaca gtcagcatgt
     2341 ggcctcttgg atcttttgtt gggtcagaat atactcc
//
LOCUS       DASVEV010000151         8699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1414733, whole genome shotgun sequence.
ACCESSION   DASVEV010000151 DASVEV010000000
VERSION     DASVEV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414733"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF222_06940"
     CDS             <1..232
                     /locus_tag="VF222_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX7142197.1"
                     /translation="MSPKMIGVTVEDGAIIAARAVIKPGVTIGKNAVVGMGAVVTKDV
                     PPDTVVLGNPARIKYSRKEYDEKKRVWEELVT"
     gene            complement(380..1525)
                     /locus_tag="VF222_06945"
     CDS             complement(380..1525)
                     /locus_tag="VF222_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142198.1"
                     /translation="MPSSPHVVVYGLSTEGYNFASSIVSAGDKVSIIDESSKMGILLN
                     KDITRTYSTASDLIEDQPLLESESMDNVISSASYLFFFPKIRKIGHEVKIDISSKFID
                     AIKPINKNTSIVYPLPTGITGNEENIDILEHTTGMTVGENLYYYYMPLSSGPLLSSTT
                     ILGNFNSKRDIFLEDMLYKFTNQEINFVDILSAELAYVSRILRHYSGIASVLEVCKFA
                     TGYGIDNRIVDNFHKDLYLDDITNGLHDLRIIQSSLHGSNPLVYLVNGTIKSIEGYTK
                     NLIDKVRYILKKRDLKASKTKISISWTLDVNEMRGDKIDVYTLLETRLRDYIGDVERR
                     GKNQLENYPSDKTTISIACSKSDFHLLSKNNSAETIIIKANPFCEVV"
     gene            1704..2828
                     /locus_tag="VF222_06950"
     CDS             1704..2828
                     /locus_tag="VF222_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX7142199.1"
                     /translation="MRIWFDILTPKQIMFFKYFVDTLKEDDHEILCTGREYREAKELA
                     RIKKIDIQTVGRHGGNNKYEKLNTSTERAFLLSSLINSFKPDLTVSFSSPEASRVAFG
                     MGIKHYIFNDSPHATAVAKLTVPLADRLFCPWVIPLREWTKLGLEKDKVTQYKALDPI
                     VWLKKELQYNKRQEIAKIQKKLKIDKEKKTVLIRPEEIKASYIADKDVKSSIGLIDEI
                     VKKFSHEYNILILARYLDQVDFYNCRFENMAGTKIIQTVMDGNLLLNLADLFIGAGGT
                     MSAEAALMGKPVISITPINFYVEKYLIKMGLIKKVRNPATLTKSIKRILSQGAMVKNK
                     NTINNNKKLSHQDTIKKMSLKIIAEMEDPIEIFKKFLFKP"
     gene            2868..2963
                     /locus_tag="VF222_06955"
     tRNA            join(2868..2877,2901..2963)
                     /locus_tag="VF222_06955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2925..2927,aa:Gln,seq:ttg)
     gene            complement(3053..4576)
                     /gene="argH"
                     /locus_tag="VF222_06960"
     CDS             complement(3053..4576)
                     /gene="argH"
                     /locus_tag="VF222_06960"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7142200.1"
                     /translation="MYRSRQKENLDKDVLNFLSSLNEDINIISYDILGTQAHCLMLNK
                     IGILTSKEVSKILLSLSKIKFESKNNKTIPKNILSNELFLSEDIHELIESIVIQDIGI
                     DIGGKMHTGRSRNDQVILDVRMKLRDDVIDICELIINVIKSLIEKSKEHKNTIISLYT
                     HLQQAQIGTVSHYFLSYSFNLLRDLERISSSFDKINLSPLGSCAIGGTGIPIDRNYTA
                     SILGFRGLLENSIDATSSRDSMIEYLSFLSILMSTLSRIAEDFILWSTSEFNYIELSD
                     RYSSTSSVMPQKKNPDPLEILRSRTSIVMGMLISSLSIIKNLPSGYHRDLQDLKPLLS
                     NSTKIVKESLLIMKGIIDTLKVNKKNTYNSANKSYGVSLDIAEQIVIKYNIPFRKVHK
                     LVGALVQHAIKNENISLSQIPKEDLNNILNLTKIKIDVDELMLIIKDTTPEKSILFRK
                     SYGSPNPVQQNEMVNSIEKSLLVYCKVVSERKELLKTAFDNLEKEVILNTNIDNKKN"
     gene            4710..5054
                     /locus_tag="VF222_06965"
     CDS             4710..5054
                     /locus_tag="VF222_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX7142201.1"
                     /translation="MSQDMQQTRRLIFDELSKIVDPEIGVSIMELELIDKVDIEEKDV
                     NVDFHLTSPFCPAVFGFKIAQDVRDNIYKIEGIENVKINVSNHFMAEAINKQVNESKP
                     PAKEEVDHQNKQ"
     gene            complement(5302..6204)
                     /locus_tag="VF222_06970"
     CDS             complement(5302..6204)
                     /locus_tag="VF222_06970"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7142202.1"
                     /translation="MNIFKKDKRKEIKKNEEPLETSNTSGSNQSPVTLRVFRINAIKD
                     PNDVHHFDEFQVPVKRWTTVLDALLDAKSYQDSSIAIRYSCRMASCGSCGMKINGIPR
                     LACYTKISDLDTNTITCEPLPNFPYIRDLVTDFSQFFKHHRSVMPYLQNKKADIPDVN
                     ELSEYEQTPEDLDKFLQFSYCIKCGLCYSACPTVATDLKFPGPQALSQAYRYFADSRD
                     SDKSNRLKVIDTSHGIWRCHFAGSCSNVCPKGVDPALGIQLLRSHLLGFSNSEKKIAK
                     LQDRSSSSYMEEEEEKEEKEKKNN"
     gene            complement(6225..6647)
                     /locus_tag="VF222_06975"
     CDS             complement(6225..6647)
                     /locus_tag="VF222_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX7142203.1"
                     /translation="MTSSNIENNSNKNKNRTKWFSNITILRESQIMKIHYFTGVGALF
                     VVAIHIMMRLIMPFNMSLEFENVILNYKNIFYALLLESILILIAIHGFNGLRVILLEI
                     RQTLIWERAVNILVIVALIGIIAYGTRTIIIANGFSLG"
     gene            complement(6656..7138)
                     /locus_tag="VF222_06980"
     CDS             complement(6656..7138)
                     /locus_tag="VF222_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX7142204.1"
                     /translation="MTSTTSSAKRRSDENNKNDRTNREGVKGWLNPIIYGWERVSYWF
                     QRLTGIFLLVYFIGHVYETSSLVSGPDAWAAMLEMTQTIGGHLFLILVIGASTFHTIN
                     GIRLIFTQSGQGLGKPGRPDYPYDPTSINNKQRSGLWIAFILAAVAMLYGANVLFSGE
                     "
     gene            complement(7140..>8699)
                     /locus_tag="VF222_06985"
     CDS             complement(7140..>8699)
                     /locus_tag="VF222_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7142205.1"
                     /translation="TAAVLFEDEGDNIESHIYDTIKGSDFLADQDVAEKLVKTIPHEV
                     YQLDHWGMPWSRRKDGRIGQRKFGGYSFPRATYAQDKVGFYEMQTLYDTCLKYDNINF
                     YNEWYCTSILKEDNLFRGLTAIEMKNGNFIKIKSPAGIICTGGAGRIYSFSTYAYSST
                     PDGIDMAYRAGLAIKDMEFVQFHPTGILPSGILITEGARGEGGYLINNQNERFMKKYA
                     PEKLELAPRDVVSRSMIREIQEGNGFKHESGFDCLKLDLTHLGDEQIKERLAGIREIG
                     IKFSGIDVIDAPIEIRPVCHYMMGGIHTDIDGKTEMGGLWAAGEAACNSTHGANRLGA
                     NSTSECIVWGRLTGEQAAKFAIDKKGSTIDVSNDQVISEEKRIYDGIFRGRGEVNPYE
                     LRKELTDTLDNKAYVFRTEETLVSGLKRIRELKTSSWKHVDDKSKEYNTNFTNVMEID
                     AMLRTAEVVLMGAINRKESRGAHARLDYPNRDDVNFLKHTLAYYNSEDEPKLAWHPVT
                     FTRYAPVERKY"
BASE COUNT         2883 a         1441 c         1304 g         3071 t
ORIGIN      
        1 catgagtcca aaaatgatag gagtgactgt agaggatgga gcaataatcg ctgctagagc
       61 agtaataaaa cctggggtta ctattggaaa aaatgctgta gttggtatgg gtgctgttgt
      121 aacaaaagat gtgccacctg acacagtcgt tcttggcaat cctgctagga ttaaatattc
      181 cagaaaggaa tatgatgaaa aaaagagagt gtgggaagaa cttgtcactt agttaattag
      241 attaacaaga gatttaaata atgaccaatt taatctcaca aatcagatac cattctaaaa
      301 gttggcagaa tattactacg aaataataat aataataata ataataatta ttttattatt
      361 ttattacttt attagtatat tatactactt cacaaaaagg gttggcttta ataataatcg
      421 tttctgcact attatttttg gatagtaaat gaaaatcact tttagagcat gcaatagata
      481 tagtagtttt atcacttgga taattttcaa gttgattttt tcctcttcgt tccacgtctc
      541 ctatatagtc tcttaacctt gtttctaaca gtgtatatac atctatttta tctcctctca
      601 tctcattgac atctaaagtc caggagattg aaatcttagt tttacttgct tttagatctc
      661 tttttttgag aatgtatctt actttatcaa taagattttt agtataacct tctatgcttt
      721 tgatcgtacc attaacaagg tatacaagtg gattagaacc atgtaacgaa gactgaataa
      781 ttctcagatc gtgtaatcca tttgttatat catctaaata aagatcttta tgaaaattat
      841 ccacgattcg gttatctata ccatatccag ttgcaaattt gcaaacttct agaacacttg
      901 caattccaga ataatgacgt agaattctgg aaacataagc caattcggca gatagtatat
      961 caacaaaatt gatttcttga ttagtgaatt tatataacat atcttctaaa aatatatctc
     1021 tcttagaatt gaagttaccc aaaatagtag tactagacag aaggggacca gaactaagag
     1081 gcatataata gtaatataaa ttctctccaa ccgtcatgcc tgttgtgtgt tctaaaatat
     1141 ctatattttc ttcattcccc gttattcccg taggaagtgg atagacaatg gatgtgtttt
     1201 tattgatagg tttgatagca tcaataaatt ttgaagatat atcaatttta acttcgtgac
     1261 caatctttct tattttagga aaaaagaaga gataagacgc cgaagatatt acgttatcca
     1321 tagactctga ttcaagtaac ggttggtctt cgataagatc cgatgcagta gaatatgttc
     1381 tagtaatatc tttatttaat aagattccca tttttgatga ttcatcaata attgagactt
     1441 tgtcccctgc ggatacaata gatgaagcaa agttatagcc ttcagtactt aagccataaa
     1501 caacaacatg gggagaagag ggcactttat caatcagata taaataagag ctccttagta
     1561 aagttattca tttcttttag ctaataattc tacagtagaa aagacaattc ttattaaaac
     1621 acttattaaa atttactcta acaattatct cattgactaa aaaagaaaag tatattcaat
     1681 gatgttatta cgtatttata ttattgagaa tttggtttga tatacttact ccaaagcaaa
     1741 taatgttttt caaatatttt gtagatactt tgaaagaaga tgatcatgaa attttatgta
     1801 ctggaagaga atatagagaa gctaaagaat tagcaaggat aaagaaaata gatatacaaa
     1861 cagtaggtag acatggtgga aataacaaat atgaaaaatt aaatacaagt actgaacgag
     1921 ctttccttct ttccagttta ataaattctt tcaagccaga tttgaccgta tctttttctt
     1981 ccccagaagc atcaagagta gcgtttggaa tgggaataaa acattacata tttaacgatt
     2041 cacctcatgc tactgcagtc gctaaattaa cagttccact agctgataga ttattttgtc
     2101 cttgggttat tcctttgcga gaatggacaa aacttggttt agaaaaagat aaggtaactc
     2161 aatataaagc acttgatcct atagtttggt taaaaaaaga attacaatat aataaaagac
     2221 aagagattgc aaaaatccaa aagaaattaa aaattgataa agaaaaaaag actgtgttaa
     2281 ttaggcccga agaaataaaa gcatcatata ttgctgataa agatgtaaag agttcgattg
     2341 gtttaattga tgagatagtt aaaaaattct ctcatgaata taatatttta attctagcaa
     2401 gataccttga tcaggttgat ttttacaatt gtagatttga aaacatggct ggaacaaaaa
     2461 taatacaaac tgtaatggat ggtaatctat tactaaacct tgcagattta tttataggag
     2521 ctggaggaac aatgagcgct gaagcagcac taatgggcaa gcctgtcatt tccataactc
     2581 caattaattt ttatgttgaa aaatacctta taaaaatggg attaattaaa aaggtgagaa
     2641 acccagcgac tttaactaaa tctataaaaa gaatcctctc ccaaggtgca atggtaaaaa
     2701 ataaaaatac tataaataac aataaaaaat tgtctcatca agataccatt aagaaaatgt
     2761 ctttaaaaat tatagcagaa atggaagacc ccatagaaat attcaaaaaa tttctcttta
     2821 agccataaca ttataatcct agtgatttac acgacattat gcccggaagc ccggtggagg
     2881 aaacattatt ttccgccgac tgtagcggcc aagcataaag gcctttggag cctttgaccc
     2941 cagttcgaat ctgggccggg ctataaagta tttatttaat taaatttttt aattttctgt
     3001 ttatggaaac aaaaaataat tatataagag tatggaattc ttaaaattca atttaatttt
     3061 ttttattatc aatattagta tttaaaataa cctctttctc taagttatcg aatgctgtct
     3121 ttaacaattc ttttctttct gatacgactt tacaatatac taaaagtgat ttttcgattg
     3181 aatttaccat ctcattttgt tgcactgggt ttggagaccc ataagatttt cggaataata
     3241 ttgatttttc aggagttgtg tccttaatga ttaacattaa ttcatccaca tctattttaa
     3301 tttttgtaag attcaatata ttattgagat cctccttagg tatctgactt aatgaaatat
     3361 tctcattttt tatagcatgt tgtactaatg caccgactaa tttgtgtact tttctaaatg
     3421 gaatattgta tttgattaca atctgttctg ctatatccag ggatacacca tagcttttgt
     3481 ttgcagaatt ataggtattt tttttgttca cttttaaagt atcaataata cctttcatta
     3541 taagtaaaga ctctttaaca atttttgttg agtttgacaa aaggggttta agatcctgta
     3601 aatctctatg atatccagat ggaagattct ttattataga gagtgaagat attagcattc
     3661 ccataacaat tgaagttcga gatcgaagta tttcaagtgg atcaggattt ttcttttgtg
     3721 gcataacgct agatgtcgag ctatatctgt ctgatagttc aatataatta aattcagaag
     3781 tagaccataa aataaaatct tcagcaattc tactgagcgt actcatcaag attgaaagaa
     3841 aagacaagta ctctatcatt gaatctctcg aactagtagc atcgatagaa ttttctaata
     3901 atcctcgaaa acctaaaata gaagctgtat aatttctatc tattggtatt cctgttccac
     3961 caatggcgca agatccaagt ggcgataaat taattttatc aaaggaggaa cttattcgct
     4021 cgagatctct taagagatta aaagaatatg ataaaaaata atgagaaact gttcctattt
     4081 gagcttgttg taaatgagta tatagtgata ttattgtatt cttatgttct ttggattttt
     4141 ctattagaga tttaataaca ttgataatta attcacaaat atcaattaca tcatctctca
     4201 atttcatcct tacatccaaa atgacttgat catttctgga tcgccctgta tgcatttttc
     4261 ctcctatatc tattccaata tcttgaataa ctatggattc tattagttca tgtatatctt
     4321 ctgaaagaaa tagttcattt gaaagtatat tcttcggtat cgttttatta ttttttgatt
     4381 caaattttat tttacttaaa gataaaagaa ttttagatac ttccttcgat gtcaaaattc
     4441 caattttgtt taacattaga caatgagctt gagttcccaa aatatcataa ctaattatat
     4501 taatatcttc attaagagaa gaaagaaaat ttaacacatc tttatccaaa ttttcttttt
     4561 gtctcgagcg atacaaaata tcaatcatta taaagtgaaa aaagatataa tatattcatt
     4621 attcttcaac aataactatt gccacaatct gaaaataata tgtgagtatt taataaatag
     4681 ttaaatatgg tttttgatta ctttatataa tgtctcaaga tatgcaacaa acccgtaggc
     4741 taatttttga tgaattatca aaaattgtag atcctgaaat aggtgtatcc attatggaat
     4801 tagaattgat tgataaagta gatattgaag aaaaagatgt aaatgtggat tttcatttaa
     4861 caagtccatt ttgtcctgcc gtatttggtt ttaaaattgc tcaagatgta cgagataata
     4921 tttataagat agaaggaatt gaaaatgtaa agattaatgt aagcaatcat tttatggctg
     4981 aagctataaa taagcaggtg aatgaaagca agcctcctgc taaagaagaa gtggatcacc
     5041 aaaataaaca ataataataa gaaattctgt ttgttatttt taaatagtta gattactatc
     5101 tttatttttt atttatacta ttttttatta tattttgttt tattttattt tattttatct
     5161 tcttttatcg ttactacacg taaatttagt tgatagattg taaaagataa taatcgttgt
     5221 agtttagaac acggttagta gaagaaagaa actatagggc gacaaatgtt attttatcga
     5281 ctatattgtt aaattattta tttaattatt tttcttttct ttttcttctt tttcttcttc
     5341 ttcttccata taagaacttg aagatctatc ttgaagtttc gcaatttttt tttcgctatt
     5401 ggagaacccc aatagatgac ttctaagaag ctgtataccc aaagctgggt ctactccttt
     5461 aggacaaaca ttgctgcaag aacctgcaaa atgacatctc catattccat gactagtatc
     5521 aattactttc aatctatttg acttgtctga atctcgtgaa tcggcaaaat atcggtatgc
     5581 ttgagataat gcttgtggcc ccggaaattt tagatcggtg gctaccgtag gacaagctga
     5641 ataacataaa ccacacttta tacagtacga gaattgaaga aatttatcca aatcttcggg
     5701 agtctgttca tattctgaaa gttcatttac atcgggaata tcggcttttt tattttgtaa
     5761 gtatggcatg acactacgat gatgtttaaa gaattgcgaa aaatcagtaa ctaaatctcg
     5821 aatatatgga aagttaggaa gaggttcaca ggtaattgta tttgtatcaa gatcagaaat
     5881 ctttgtataa cacgccagtc tcggaatccc atttattttc attccacaag aaccacagga
     5941 agccattcta caggaataac gaattgcaat actactatcc tggtaacttt tggcatctaa
     6001 tagtgcatct aatacagtag tccatctttt tactggcact tgaaattcat caaaatgatg
     6061 aacatcatta ggatctttga ttgcattaat cctgaaaaca cgtagtgtta cagggctttg
     6121 attactgccg gatgtatttg atgtttctaa aggttcttca ttcttcttta tttcttttct
     6181 tttatctttt ttaaatatgt tcatattaca atagcaccct catattaacc caaagagaat
     6241 ccattagcga tgattatcgt tcgagttcca tatgcaatta ttccaatcag tgcaactatt
     6301 actaatatat tcactgctct ttcccaaatt agagtttgtc ttatttctag taaaataact
     6361 ctaaggccat taaagccatg tatggctatt agtatcaata tagattctag taataatgca
     6421 taaaaaatat ttttataatt taatataaca ttttcgaact ctagtgacat attaaatggc
     6481 attattaatc tcatcattat atgaatagca actacaaata atgcacctac tccagtaaag
     6541 tagtgtattt tcattatctg gctctctctt aaaatagtaa tatttgaaaa ccatttagtt
     6601 ctgtttttat ttttattgct attattttcg atattagagg atgtcatatg attattcact
     6661 ctccactaaa aagaacattt gccccatata gcattgccac agcggctaga ataaacgcaa
     6721 tccacaatcc cgaacgttgc ttattattaa tagatgttgg atcatacgga taatcaggtc
     6781 ttcctggttt tcctagccct tggccagact gagtaaatat caatcgtatt ccatttattg
     6841 tatgaaaagt actggctcct attacaagaa ttagaaatag atggcctcct atagtctggg
     6901 tcatttctaa cattgcagcc caggcatctg ggccgctcac taacgagctg gtttcataaa
     6961 catgaccaat aaaataaact aagagaaata taccggttaa tctttgaaac cagtaagaaa
     7021 ctctttccca tccatatata atcggattga gccatccttt gactccttct ctatttgtac
     7081 gatcattttt attgttctca tcactccgtc gttttgctga tgatgtagtc gatgtcatat
     7141 caatatttcc tctccaccgg agcatacctt gtaaatgtga ctggatgcca agctaatttt
     7201 ggttcatctt cagaattata ataggctagt gtatgtttta aaaaatttac atcatctcga
     7261 tttggataat caagtctggc atgtgctcct ctagactctt ttctgtttat cgcacccatc
     7321 aagactacct ctgctgtcct caacatagca tcaatctcca tcacattagt aaaattagta
     7381 ttatattcct ttgatttatc atcaacatgt ttccagcttg atgttttcaa ctctcgtatt
     7441 cttttcaatc cagaaacaag agtttcttct gttcggaaaa catatgcttt gttatccagg
     7501 gtgtccgtta attcctttct caattcgtag ggattaactt ctcctcttcc tctaaaaatg
     7561 ccatcataaa tccttttttc ttctgaaatg acttggtcat ttgatacatc aattgtagat
     7621 ccttttttgt ctattgcaaa tttagctgcc tgctctccag tgagtcttcc ccaaacgata
     7681 cattctgatg ttgaatttgc ccctaatcta tttgccccat gtgtactatt acatgcagcc
     7741 tctcctgcgg cccataaacc tcccatttct gttttcccat caatatcagt atgaatccct
     7801 cccatcatat aatgacaaac aggtctaatt tcaataggag cgtcaattac atcaatacct
     7861 gaaaatttta ttccaatctc tcggattcca gctagtcttt ctttaatttg ttcgtctccc
     7921 aaatgtgtta gatccaattt taaacaatca aaacctgatt catgtttgaa gccattccct
     7981 tcttgaattt ccctgatcat cgatcttgat acaacatctc tgggagcaag ctctaatttt
     8041 tctggagcgt attttttcat aaaacgttca ttctgattgt ttatgagata gccgccttct
     8101 cctctagctc cttcagttat taatattcct gagggtaaga tacctgttgg atggaattgc
     8161 acaaattcca tgtctttaat tgccagtcca gcccggtatg ccatatcgat accgtccggc
     8221 gtagaggaat aagcataagt agagaagctg taaattcttc ctgcacctcc tgtacatata
     8281 attccggctg gagactttat tttgataaaa tttccatttt tcatttctat cgcggttaat
     8341 ccccggaata aattatcctc cttaagtatg ctagtacaat accattcatt gtagaaatta
     8401 atattatcat attttagaca tgtatcgtat aaggtttgca tttcataaaa gcctacttta
     8461 tcctgagcat atgtagcacg ggggaacgaa taaccaccaa attttctttg tccaatacgt
     8521 ccatcttttc tccttgacca cggcatgccc caatgatcta attgatacac ttcatgaggt
     8581 attgttttta ctagtttttc agccacatct tgatccgcta aaaaatcact tcctttaatt
     8641 gtatcatata tatgagattc tatattatct ccttcatctt caaacaaaac tgctgcagt
//
LOCUS       DASVEV010000152         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1026146, whole genome shotgun sequence.
ACCESSION   DASVEV010000152 DASVEV010000000
VERSION     DASVEV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026146"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1420
                     /locus_tag="VF222_06990"
     CDS             503..1420
                     /locus_tag="VF222_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7142206.1"
                     /translation="MQAIEAYNLSKIYKGTHVPALDNISLKIDQGQIFTLLGRNGAGK
                     TTFVRICATQLLPTSGTLKVSGYDVISQAENIRKFISVVPQEGRPLRALTPWDHVYNW
                     LKIRGEEKSIAKIKTEKILERFELLKVKDKPAMNLSGGMKQKILVAMATAVDADLLFL
                     DEPTIGLDPVSRRQVWNLMQDLRKEGKTILLTTHYMDEAEILSDKIVILDNGKIVKEG
                     NINDLRKIIPQNIRMDISKKNIVDVEKLKDYGNVVEIGTDIIRLFTFESNIGELSNLA
                     IKNNMSFNISPITLDDIFVYLVGNTIKNN"
     gene            complement(1486..2094)
                     /locus_tag="VF222_06995"
     CDS             complement(1486..2094)
                     /locus_tag="VF222_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142207.1"
                     /translation="MSNKKKKNNENDDNKKKKSYFDISRKKRSKNFKTVILPIIAAIV
                     IIAVISMYVSSQNTLSVAGFGPLGSAHEHAAFLVKVNGQNIDFSQPKYQVQSDYIHVE
                     NGDGSTLHRHATNVTFADFLKSVKMNIDKKNNCLIFTNGTEYCDDNNNKLRTFINGNS
                     TKSISDYVLNNNDRLLVIYGNETDEQIAKALDELNKIKIQFN"
     gene            2684..3715
                     /locus_tag="VF222_07000"
     CDS             2684..3715
                     /locus_tag="VF222_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7142208.1"
                     /translation="MFFFISPIGLGHATRDVAIIHNLKFKIDNKVNFVTGSAAYAFLT
                     SYGLKAFNLYDPPKFEIESGKLRHSFTWLIKYLLYYNKCKEIATKIIDANIEKLIVSD
                     EDFASISIAEEKNLRRILITDIVKTSFTKGITSIIEKEMNKRLCKIIKKCDCVIIPDY
                     GCDTENFRYVGPIVREILESRETLRKRFNFTKKTIVVSVGGTDAGKYLIEKVIDSFRR
                     LKAKLDIDLIVVTGPALQINPAEDFHLLGYVPNLNEYIYASDLIVSLAGRSTMDESIV
                     YNIPGIFIPLKNHYEQEESAKRLGFEYKDILRLDKLIEEKLSSNSDIRKNNVSNGAKK
                     AAEIISSFI"
     gene            3767..5242
                     /locus_tag="VF222_07005"
     CDS             3767..5242
                     /locus_tag="VF222_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7142209.1"
                     /translation="MTEVIIAKFGGSTIGQNGDQIPTVIERIKAMRKEGKKIVAVFSA
                     PLMEHEGKVKSMTDIAINIGRKYASGQKVDTNILYRTYLNIAKKYLSQKYFEEFEFHL
                     EMFYNYVTTSLNHVTENRRFIDVTRSRILAYGGEITMSYLMDYVMRSNGLKCDHVDIE
                     KWPIITDDNFEAANFMIEESKEHKEHFLKLLEENEVISIGGFIGKTVDGLETTYERGG
                     SDRTAADIAIILYDEFKVQIDFEKDNIVLSADPKIVKEELESIRYLSYNEAKLAGMFG
                     MKILDPIAIKEIDDNNFDIPIVITSILNPSDITIIEKSLINRKHDFLDSVENNDDDSS
                     LIKIVTGKRNCAIVRMESIAASYLIASLEKDKQYHNFLKLSPFKVDDIEITRLLFLDA
                     DYVKRHERHFKAFYPKVEFAYGKGVVTLIGDMMWKMPKIVSTASRTVGDYDINILNLD
                     AQEETSRILIIVDDIDNNVANAIRAIHLQRNKSDLKITHNN"
BASE COUNT         2050 a          642 c          831 g         1794 t
ORIGIN      
        1 tgttattttg aagtttccgt caataatttg aataatatat ttattatttt taaaaaataa
       61 ttcaagtttg tcttctgttt ctttgatatc cacaagttct tgacctttca tatcagtaaa
      121 ttctaccata aatttatgac gagttactat tatttagatt atttgattgt ttataaataa
      181 atatatcaat atgaattaat tactacaaaa attatttaaa atactttatt tttagttcca
      241 tatgtaattc tggatctagc ctcttaatta tcatatccat tttttcttct tttctttcgt
      301 ctccttcttt agattgaggt gatcctacat accaaagaat tgaaagtcct gctacctgtc
      361 atataactgt aagaattctg attgccaatt ttccatagta tctcgaaaag tcatattgaa
      421 gtaaccagaa atttcaactg gttgagattc aaatatattg attgaaagta aatatattga
      481 tagaaagtaa atactaaaaa tattgcaggc aattgaagca tataatttat cgaaaattta
      541 taaaggcaca cacgtgccag ctttagataa tatctcatta aagatcgatc aagggcaaat
      601 ttttacatta ttaggaagaa acggtgcggg aaaaactacg tttgtaagaa tatgtgctac
      661 tcaattgtta ccaacctcag gtactttaaa agtttcaggt tatgatgtaa taagccaagc
      721 agaaaatata aggaaattta tctcggtggt tccacaagaa ggaagaccat tacgagcact
      781 tactccttgg gaccatgttt ataactggct caagattaga ggagaagaga agtctatcgc
      841 aaaaataaaa actgaaaaaa tattagagag atttgaattg cttaaagtaa aagataaacc
      901 tgcaatgaat ttgtctggag gaatgaaaca aaagattttg gtagcaatgg ctactgctgt
      961 tgatgcagat ttattatttc tagacgaacc tacaataggt cttgatcccg tttcaagacg
     1021 tcaagtttgg aacttaatgc aagatttgag aaaagaaggt aaaactatat tacttacaac
     1081 acattatatg gatgaagcag aaatattatc tgataaaata gttatactcg ataatggtaa
     1141 aattgtaaaa gagggaaata ttaatgattt acgaaagata attccacaaa atattcgtat
     1201 ggatatatct aaaaaaaata ttgttgatgt tgaaaaattg aaagattatg gaaatgtagt
     1261 agaaataggt actgatatta ttcgattatt cacctttgaa tctaacatag gtgaattatc
     1321 taatctagct ataaaaaata acatgtcctt taatatatca cctataacac ttgatgatat
     1381 atttgtatat ttagtaggaa atactataaa aaataattag agtcaattat tattcattta
     1441 acctaaagga tattaaaaga ttaaggaaat agctaaaaag aaaatttaat tgaattgaat
     1501 tttaatctta ttcaattcat ctaatgcttt agcaatctgt tcatcggttt cgtttccata
     1561 aatcactaac aaacgatcat tattattaag gacataatct gaaatagact tcgttgaatt
     1621 tccattgatg aatgttctta gtttattatt attatcatcg caatattctg ttccattagt
     1681 gaagatcaga caattatttt tcttatctat attcattttg acgcttttta aaaagtccgc
     1741 aaaagtaaca tttgttgcat gtctatgcag tgtactgcca tcaccgttct caacatgaat
     1801 ataatcagat tgtacttggt attttggttg actaaaatct atattttgac catttacttt
     1861 aactaaaaat gcagcatgtt catgtgcaga acctaaaggt ccgaaccccg caacagatag
     1921 agtattttga gaagatacat acattgaaat tacagcaata ataacaattg cagctataat
     1981 tggaagaata actgttttaa agtttttact cctttttttt cttgatatat cgaaatagct
     2041 ctttttcttt ttattatcat cattttcatt gttctttttc tttttattcg acatgataaa
     2101 ttgattgatt tgagtaatat cgtattatat agcactttcg gaaacatgga aaataaacta
     2161 tttttgagaa tataatttca aatttatagt aaatttttta caagtaatat gatcgtgatt
     2221 actgctatct ttcatttgat cttcatatta ggaattaata gtgatagtac attcaattta
     2281 agcaataatt tataataccc tgctccaatt tttattatat ttcaatcatt atttaaaatc
     2341 tatattaaat atttgtgtct aactgtagta ttatttcttt tacaatagga actatacact
     2401 atgtgaggta tattttaaat gatcatcttt aattaattta atataattaa agtactacta
     2461 gaaaattatc tcatgttact atgaccaaac atgtatcgtc aatcgtcatt atcaagttaa
     2521 agtttttttt tgattggaac aagtaaacac tataaacttt atttagtaaa acaattcgct
     2581 aatttacaag gtaaaaatag aatttaaaaa aaccaactaa tagagaaaaa ttaattaatt
     2641 tagacagtaa catacaaaaa gaaggaaaca aatgtataga taaatgtttt tttttataag
     2701 ccctataggt cttggtcatg caacaaggga tgtagcaata attcataatt taaaatttaa
     2761 aattgataat aaagtaaatt ttgttaccgg ctctgcggca tatgcatttt taacaagtta
     2821 tggattaaaa gcctttaatt tatacgatcc acctaagttt gaaattgaat caggaaaact
     2881 acgtcattca tttacatggt taataaagta tttgttatat tataataaat gcaaagaaat
     2941 cgcaacaaaa attatagatg cgaatatcga aaaactaatt gtcagtgatg aagactttgc
     3001 atcaatttcg atagcggagg aaaaaaattt aagaagaatt ctaattacag atatagttaa
     3061 gactagtttt accaaaggta taacatcaat aatagaaaaa gaaatgaaca agagattatg
     3121 caaaattata aagaaatgtg attgtgtaat aataccagat tatggatgtg atacagaaaa
     3181 ttttagatat gttggaccaa ttgtaagaga aattctggaa agtagagaaa cattacgtaa
     3241 aagatttaat tttactaaaa aaacaattgt tgtaagtgta ggtggaactg acgctggaaa
     3301 atatctgata gaaaaagtta ttgattcttt tagaagatta aaggcaaaat tagatataga
     3361 tcttattgtt gtaactggcc ccgctctgca aataaaccca gctgaagatt ttcatttgtt
     3421 aggatatgta cctaatttga atgaatatat ttatgcttct gatctaatag tatcattagc
     3481 gggtagatct acaatggacg aatcaatagt ttataacatt ccgggaatat ttatacctct
     3541 taaaaatcat tatgagcaag aagaaagtgc caaaaggcta ggctttgaat ataaagatat
     3601 attaagactt gataaattaa ttgaagaaaa acttagttca aattctgata taagaaaaaa
     3661 taatgtgagc aatggagcaa aaaaagctgc tgagatcata tcaagtttta tttagatgtt
     3721 atacattaat aatataacga aaattacaat aggataggta taacatatga ctgaagttat
     3781 tattgcaaaa tttggaggaa gtactattgg ccagaatggt gatcaaatac ctactgtgat
     3841 agaaagaatt aaggctatga gaaaagaggg aaaaaaaatt gtagctgttt tttctgcacc
     3901 attaatggaa catgaaggaa aagtaaaatc tatgacagat attgcaataa acattggtag
     3961 aaaatatgct tctggtcaaa aagtagatac aaatattttg tatagaacat atttaaatat
     4021 agccaaaaaa tatttatctc aaaaatattt tgaagaattt gaatttcacc tggaaatgtt
     4081 ttataattat gttacaactt cgttaaatca tgttactgaa aatagaaggt ttattgatgt
     4141 tactagatca agaatacttg catatggagg agagattaca atgtcatatc ttatggatta
     4201 cgtaatgaga agtaatggat tgaaatgtga ccacgttgac attgagaaat ggccgatcat
     4261 tactgatgat aattttgaag cagctaattt catgatagag gaatctaaag aacataaaga
     4321 acactttctt aaattactag aagaaaatga agtaatatct attggaggat ttattggaaa
     4381 aacagttgac ggacttgaaa caacatatga aagaggagga tctgatagaa ctgcagcaga
     4441 tattgcaatc attctatatg atgaatttaa agtacaaata gatttcgaaa aagataacat
     4501 tgtcctaagc gcagatccaa aaatagtaaa agaagaattg gaatctatac gatatttatc
     4561 atataatgag gcaaaattag ctggaatgtt tggtatgaag attttagatc ctattgcaat
     4621 taaggaaatt gatgacaata attttgatat ccctatagta attacatcaa tattgaatcc
     4681 ttctgatatt actataattg agaaatcttt gattaataga aaacacgact ttttagatag
     4741 tgtagaaaat aatgatgatg atagttcatt aatcaagatt gtaacaggaa aaagaaattg
     4801 tgctattgtc agaatggaaa gtattgctgc ttcttattta atagcatcac ttgaaaagga
     4861 taaacaatac cacaatttct taaaattatc gccgtttaaa gtagatgata tagaaattac
     4921 aagattgcta tttcttgatg cagactatgt caagcgtcat gaacgtcact ttaaggcttt
     4981 ttatccaaaa gtcgaatttg cttatggaaa aggtgtagtt acgttaattg gagatatgat
     5041 gtggaaaatg ccaaaaattg tttctactgc aagcagaact gtaggcgatt atgatataaa
     5101 tatactgaat ttggatgccc aggaggaaac atcaagaatt cttataatag tagatgacat
     5161 agataacaat gttgcaaatg ctataagagc aatacattta caaagaaata aaagcgattt
     5221 aaaaattact cacaacaatt aaactttatt agtattaaag aatgaaaatt aaatggacga
     5281 gagaaatatt atttggatcg atggtctttt atatgaa
//
LOCUS       DASVEV010000153        15406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_831686, whole genome shotgun sequence.
ACCESSION   DASVEV010000153 DASVEV010000000
VERSION     DASVEV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831686"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..260
                     /locus_tag="VF222_07010"
     CDS             48..260
                     /locus_tag="VF222_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142210.1"
                     /translation="MSNNKDIVTPAEEEYEENFKIEISTQKGSSIRVETSSYNKLDNL
                     IDKALTTYTRLTSEESGQVEQQQQYQ"
     gene            complement(305..832)
                     /locus_tag="VF222_07015"
     CDS             complement(305..832)
                     /locus_tag="VF222_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7142211.1"
                     /translation="MKIILATTTLIALLTIGIIGINNTIGNAFAQISSEDLPMEEMID
                     DETVTMTMSNQSEPMVNWTGTIEVESTIAEALKSKVNVNMIDAIKAAQENVGLNSTVK
                     EAKLTLAHNYLVYKIMVVDEDMKKYKFIVDPGNGEILMKKEITWYDDHAKMNCEGMGD
                     KSGHDYKMMMKDKKY"
     gene            1353..1838
                     /locus_tag="VF222_07020"
     CDS             1353..1838
                     /locus_tag="VF222_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142212.1"
                     /translation="MVEKELIEQKIGEAIGLEKAAQKAVDELKSKGLLKTEHEKKLMP
                     MKEEANKQQREMEELIEEIAENEGFDVENINSVSEETAQKASKMMETYLGEEPDTQEA
                     LEFLCLAEGAEVTHYEVLASVAKEVKSRKFGNQVRKILKEEHNHLEACTKLAKQNVTK
                     E"
     gene            2495..2743
                     /locus_tag="VF222_07025"
     CDS             2495..2743
                     /locus_tag="VF222_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142213.1"
                     /translation="MRDNYGGVLKPESIIKKEARGIDNTDLGEIKEITFDCIITQKDT
                     VNKDKFIIPKDLLERFHEDNILFQITEEETETYRRKTD"
     gene            complement(2985..3335)
                     /locus_tag="VF222_07030"
     CDS             complement(2985..3335)
                     /locus_tag="VF222_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7142214.1"
                     /translation="MKKIEIIIPHERIDNANRILNKFGIRDEMSFYQINGRREKTEVT
                     SVPVGRSIIMYTPKYGGRTKIEALVEDSLVKKLLEELLNKLTTGSVSDGKIFVYDASE
                     VYDIGSGKKTELNI"
     gene            4040..4447
                     /locus_tag="VF222_07035"
     CDS             4040..4447
                     /locus_tag="VF222_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7142215.1"
                     /translation="MLNRIEGITLLVSDIKRSVAFYRDILGLRVNKEGEDKVEFSRKD
                     STNTKLILELENSESNQNNKNSIIITFSVSDLNTIYNNLTQQKVNFHKKLSDDASGKN
                     TIILDPDGYLISLTEPASDEYAQIPYYHGFAPA"
     gene            4884..5381
                     /locus_tag="VF222_07040"
     CDS             4884..5381
                     /locus_tag="VF222_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7142216.1"
                     /translation="MDNILKIIIVTFLGILISHVFTVEFVNSQPVSMEGTSSNDKIIG
                     REGNDNIEGMEGNDALDGWFGDDEIEGGEGNDNLKGGKGNDKLYGDEGNDILKGYLGN
                     DSLVGGEGDDKLEGGKGGDLLEGGNGGDSFICDSDDVIVDFNHQDGDSIEGPCRYDYI
                     PKQLY"
     gene            complement(5665..5793)
                     /locus_tag="VF222_07045"
     CDS             complement(5665..5793)
                     /locus_tag="VF222_07045"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX7142217.1"
                     /translation="MEVMCKKCKAHLGHVFDDGPTPTGNRFCINSASLKFEKDRLK"
     gene            6136..7317
                     /locus_tag="VF222_07050"
     CDS             6136..7317
                     /locus_tag="VF222_07050"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX7142218.1"
                     /translation="MSDSNIPSNITSITDFPFLDIPASLVEEMLSKTTKISNDLVNSL
                     DTIRKKRHYFRSQLDSSEILGKDTEIKSHNILDSCGIDGAFAIERLLGTDLIAIATVG
                     VEGLMSSYQKGKWDSPNHNVFINPERHDPENMIAARGLMIEMEINLAANAPYDLIFLD
                     GSLTTGLIHMYKAIDFIEKHKSVTANQIRDNYKNFLISYKKILESKDNKRQWIGIPKY
                     TSRNDIGDIYDWPTDYDDRAILTLILNEGEYTLPKPFTLKENWHKKLPTIDNGKDDYE
                     LENLMEGVIDEIKRLHVIYYKPYKWSPAIRIEISSSVANDSNRFYALLQSIKSQCSIP
                     SIMEPYPLYLADRMVKHIAPAIPAYKQVLTRSMTEIEKNDESISDIIFIMHSYRTESG
                     M"
     gene            7323..9215
                     /locus_tag="VF222_07055"
     CDS             7323..9215
                     /locus_tag="VF222_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7142219.1"
                     /translation="MISHNINNNDNNKIKNNDSDLNCDIKLNQLLSNTEPIGTIGSPS
                     STSEISLNILQSAVTKKLIGELVIFRYLQESKSHYSLGQITEIELKNVLLEDPTIKSV
                     ARQRGLVNPISGIQDTHVGKLLCSAVFSNNDEENFFDTSLLGTVPATGTYIHITNDNI
                     LNSLLEKHLDKIFYLGYVYGSQTKLPMWFKHFGNGIEGAGEAYHLGIFGTTGSGKSTL
                     AKMILMAYAKYPEMGLFVFDPVGEFSKDAMFDNANGLNYQSKNNGFILNIKEILNSLG
                     KKFEIVNIRNLILDRWELFEEILYESSFFPNLSIKGENKRDAVNVLINEIKNRLHISL
                     TNLIELESFQQVLNILRNPNIQLLIYSTAEPRARLNTVLSEVDENYLYERFWKPICQL
                     FHYRPGAQTVDSLLKRFTNDEKPLVIVDLSLQSAHHLKLDYWNETIQALILKRILYGL
                     INLGEKSWEQNTSLNTLVVLDEAHRFARREKYENSDLENLRLTLLDAVRTTRKYGLGW
                     MFISTSISSVHKDILQQLRIMFCGFGLSIGSDLATLRELVSDHNALKLYQSFTDPATS
                     FSTKSRKYSFMSQGPVSPLSFAGTPLFLNVFNSSQTFVKENKLLSYTRKSISNSLTYT
                     QDNYDF"
     gene            9278..10546
                     /locus_tag="VF222_07060"
     CDS             9278..10546
                     /locus_tag="VF222_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142220.1"
                     /translation="MRIIHISDTHLGKRPKRTRASIINQEIKPIEDDFYNSWRRFIEE
                     IINLDKDEKPDIIIHSGDFFDTPSGTDPSPPPELARKVAAEAFKKLYENNIPIVIIDG
                     NHGRYMQYRISPLTEYTILFDNVYLFTYFDIRDSIRNQQPLFKDFPTLNLRIHAHPSL
                     ESNDLPQLLLKYEEWVYIQNNNINPDMINVFLAHGMIENHTLNSNFLMGDYDYIGLGD
                     NHKMQQINDNAWYAGSTELWSFNEETYQKGYLVIDIDDSSSKSKIKVSQKLLPQQRKI
                     ISDHVEIYPDDNNSKIIDRIKKVFEKNGLDTPYNYQTAARVKITIRGNRSYGSFYNIN
                     EISSYLNKITLNSNEYNIVEFILETPKYYDDTVIKQIKQDDTFIEYLIEDPEKEFKKY
                     ITSTRKEDLKKNNLDSNVLAKLFFESLKNQ"
     gene            10563..13121
                     /locus_tag="VF222_07065"
     CDS             10563..13121
                     /locus_tag="VF222_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX7142221.1"
                     /translation="MKIESKNFKPFDNISIPCEGYLGEGFFIIKGKNSMGKTSIIESI
                     LWGLLGEHLMAEKDRKSIIKTGESKCEVAITFEISDILYRVIRTIEVIRKRKSLQDLQ
                     NDLVKSSALLFKKEKNEDKFIQLCRGPNIVNQEIESLLGVYPEVIEKTIYIRQKEVDK
                     LALADPSELRELIKNLFGLNDFDDHIKKYLKNQINSINEEIKDLKIQIGSLLTEQNEW
                     EKQKQILEEKKEKLELKLQEIKNDKNTLISYPSQEKLYKIKVSIENIEKYKKDVDYRE
                     KMYNYKKENLLVQENRIKDLQDKIFLLDSKILKLSKELQEYPPKDSIRKASDILKGID
                     NDEKQIRNLISKSNQKLNIDIQNISINNVEELEKVKEKNQIELEKTDKERISILTIME
                     GHKKLFTSNQLLYEIQNNSIKYIQDRDKCPICSSKINDKNTLLFDLKRARENIFNKEN
                     SFKKEINQLEIKLKKIEAELDEKKGVRQLIEVIVTIVNGLIEKRFNFYDELNSISQVY
                     KKYDELNNLNSRIIDDLISSVSEIENNIFSSKELVKNFKERLESENIKLEKMKKEINI
                     FNKEKNDILLKLQSNKSNLQTYCDDIKVSNIHNLLANFNCNSIDELISKRLNITVRIE
                     EKAKTIENIHGDISYLTEEIEIRGKKIISLRVAEEKLKEKEYLLKHVLFLLGEVDGFI
                     STDIVEGRLSDVLMKNTNFYLIPFTEGRYEIANISSTMRKIKDRISHGLTLTLVDNKD
                     HITKNKEQLSGGDESALGLALRISISKLMSTIRPFKHVEKKVPMINFIIMDEPMTSLD
                     EFRRNVIMNILMKDQSFKQIFLISHAEINEEENNYHSIVINDNENSKRMVEYFH"
     gene            13508..13720
                     /locus_tag="VF222_07070"
     CDS             13508..13720
                     /locus_tag="VF222_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142222.1"
                     /translation="MHVFIVLLINLAGIVSIFYIKNTRIVGIALFIYGTLLLATATFF
                     GIPGFILFVIAGIMALKEKQVIKESK"
     gene            14402..>15406
                     /gene="cax"
                     /locus_tag="VF222_07075"
     CDS             14402..>15406
                     /gene="cax"
                     /locus_tag="VF222_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX7142223.1"
                     /translation="MKFTKSSIIYFLLVFVPISVILDLVHADHIIIFIIAVIALIPLA
                     KLIGDSTEHLSTHYGSTLGSLLNVTFGNAAEIIIAVVAINAGLIDLVKASITGAILGN
                     IMLIFGLSMIAGGIRKKEQLFSRENAGLQSTMIFLAIIGLAIPTVLSSTILKPAEIEN
                     QLKIQFLSDALAIILLSVYIAGIVFTFFTHKHLFVSPHMEEGNNNHNIDTTTKHWDKK
                     KAFFMLAISMVGVVVISEILVGSVEETSKQFGFGEVFVGAIIIGIVGNAAEHSSAIIL
                     ARKGKIDLSIGIAAGSGTQIALFVVPLLVIFGIILNQPFTLEFTIYELVTLFLAAIIL
                     N"
BASE COUNT         6116 a         1882 c         2176 g         5232 t
ORIGIN      
        1 ttcttgatct tcttaaatag tatatataac aaaatatttt gataaatgtg tcgaataaca
       61 aagatatagt aacaccagcg gaagaagaat atgaagaaaa ttttaaaata gaaatttcta
      121 cacaaaaagg ttcttctata agagtagaaa catcctctta taataaacta gataatctaa
      181 tagataaagc attaactacc tacacacgat tgacaagcga agaatctgga caagtagaac
      241 aacaacaaca atatcaataa ataaaaaaat agtgattagt tgaaatcact aaactccaac
      301 ctatttaata ttttttatct ttcatcatca ttttatagtc gtgaccggat ttgtcaccca
      361 ttccttcaca gttcatttta gcatgatcat cataccacgt aatttccttc ttcatcagaa
      421 tttctccatt gcctggatct actatgaatt tatatttttt catatcttca tctacaacca
      481 ttatcttgta gactagatag ttatgtgcaa gagttaattt tgcttccttg actgtagaat
      541 ttaatccaac attttcctgt gctgctttta ttgcatctat catgttgaca tttacttttg
      601 attttaatgc ttccgctatg gtagactcta cttcaattgt tccagtccag ttgaccatag
      661 gttcagattg attagacatt gtcattgtaa cagtttcatc gtcaatcatt tcctccattg
      721 gaagatcttc tgaacttatt tgagcaaatg cgtttcctat agtgttattt attcctataa
      781 ttcctattgt tagaagagca attaaagttg tagttgcaag aattatcttc atcgaaaatg
      841 ataggttcct ctaatttata acagttgggg ggatatattc tatatagaga ataatttgtt
      901 ttactattta atactaaaaa gataaattat aatttcattc tattctgttt acataataaa
      961 tattatcaat ttcaatatat tcatagagac atatatccaa atttttattg aaagaatttc
     1021 aattaaatca tctcttgaaa ttctcaaaag aaagatttta acttttaaaa tttttctttt
     1081 aatataaatc cgtaaataca aataatggat tcaaattcca tctcttttct ttcactatct
     1141 tcataggatt tacatatttt tataaaaaag aaaaatcaaa caattaacgt taaaagcaat
     1201 ggcttaaagt tgtgctaata ttggtaatga tatctttaga gattagataa aaagattgat
     1261 attatatata agaaacttct tctattatat tttccaaatt caatattaat atcgttatta
     1321 actttattgc aatataaaca gtgaaaatat agatggtaga aaaagaatta atcgaacaaa
     1381 aaatcggtga ggcaattggt ttagaaaaag cagctcaaaa ggctgtagat gagctaaaat
     1441 caaaaggatt gctaaaaaca gaacatgaaa aaaaactaat gcctatgaaa gaggaagcaa
     1501 acaaacaaca gagagagatg gaagaactga ttgaagaaat tgcagaaaac gaaggatttg
     1561 acgttgagaa tattaattct gtttctgaag agactgctca aaaagcatca aaaatgatgg
     1621 aaacctattt aggtgaagaa cctgatacac aagaggcttt agaattctta tgtttagctg
     1681 aaggtgctga agttactcat tatgaagttc ttgcttctgt tgcaaaagaa gtaaaaagca
     1741 ggaaatttgg aaatcaagtt agaaagatat tgaaagaaga acataatcat ttagaagcat
     1801 gcacaaaatt agcaaaacag aatgttacta aagaatagca tatacatatg aagaattatt
     1861 tattttcttc ataatctaat tattttaatt acaaattgaa ggcataacct catttggaaa
     1921 actcacacag aacaaacaaa aatatctggt taatgtttat cttgaattta ttataaatat
     1981 aataaaatct agagagatat ttaactttaa attttatttt taattccgaa tttaagtgtt
     2041 agatattata gttaattttt gtagtaatag ttccaatctt attattgttc taatattgtt
     2101 aaatacaata ctaaaaaaga tattttactt tatataaaca agctttaaat ttatatgtta
     2161 cctactgaag ttactattat attatgaatg aaagttaaaa gaattgctga gatatctcta
     2221 tgctaataga agatgatagg attaagatta aaggataata aatacattct agaattacaa
     2281 aaaaaagatt acagatattg aaaaaatagt aatttatcaa ccaatcttgt tggaagaact
     2341 tgtaaataaa gtttaattac tctatcaatt caacaaaagg ataacgttga ttattatata
     2401 gtaacaagaa ttagatactt ataaataatt actgacaatt tttatatatt tttagaatat
     2461 attccccttt atacaataat gaactataat aagtatgaga gataattatg gtggcgtttt
     2521 aaaaccagaa agtatcatta aaaaagaagc gagaggtatt gataatacag accttggtga
     2581 aattaaagaa attacttttg actgtataat aacacagaaa gatactgtaa acaaagacaa
     2641 attcatcatt ccaaaggatt tgctagaacg atttcatgaa gataatatat tgtttcaaat
     2701 aacagaagaa gaaacagaaa catataggag gaagactgat taggtctttt ttttatttta
     2761 ttaaaaccat taattttcat atctttaatg tagtcaagtt gataaaaatt ctttacatga
     2821 gcctgagtat tttaataatc cttacgtcat aacttaagtt gtgtacttta taaacgactt
     2881 aggaatataa agaactccaa aatcacttaa ggagctaaaa atctcgctga aaatattggc
     2941 gaattgtaag aataaatgaa agtttgattt gtacgagtaa ttttttatat attcaattca
     3001 gtttttttac cgcttcctat atcataaact tcagaagcat cataaacaaa tattttgcca
     3061 tcagataccg aaccagttgt taatttattt aacaattctt caagtaattt tttaactaaa
     3121 gaatcttcaa caagtgcttc aattttggtt cttcctccat attttggagt atacataatg
     3181 atacttcttc caacaggcac agatgtaact tcagtttttt ctctcctacc atttatttga
     3241 taaaaagaca tttcatctct gataccaaat ttattcaaga tacgattagc attatcaata
     3301 cgttcatggg gaataataat ctcaattttt ttcattgttt atacaaatac attattttat
     3361 aatacaatat tagaatgtaa gcgataatta acaatagaat actactacta ctatgtctta
     3421 ctatattttc ttaaatttat atatttttca acacttttac ttccagtcaa taaatagaat
     3481 ttcttatata aaatgaaaat atctcatata tcttactttt aacagttatt aatcgtagat
     3541 aaatttaata gttgaataat agaatatcta aaagaaatta taaactcatg agagaaacac
     3601 tcatcaaaaa atcaattgaa agaaattata atgtataaat cgttttgact tagttttaaa
     3661 tacaattact aatataatta atccgttaaa gatgactttt tgcaagagaa aaaattcatt
     3721 atctggccct attaattaaa acaagtatat gagtatgtat ttcttgtaat aaacaaatta
     3781 atacaatttt caaataaata atttaccctt ctatttctac tggaaaaata ttaagaaact
     3841 attgttacgc tatatacttt aacaattata atccggcatt aaaaaaagtt aaaccaataa
     3901 gtaaaaaaat ttagagttga cattaattga tgacagatct tgttaaactc ttttattgga
     3961 tatgcctttt agtaattttt tgatattatt gaattttaat aacattaaat ttaataaaaa
     4021 gaatgttgta gtgacaagca tgttaaatag gatagaagga attacactgt tagtttcaga
     4081 tataaaaaga tcggtagcat tttatcgtga tattctcggc ttaagagtaa ataaagaagg
     4141 tgaagataaa gtagaatttt cacgaaaaga ttctaccaat actaaactta tactcgaatt
     4201 agaaaattca gaatctaatc aaaataacaa gaattctata atcataactt tttctgtaag
     4261 tgatttaaat acaatatata ataatttaac tcaacagaaa gttaattttc ataaaaaatt
     4321 gtcagacgac gcatctggaa aaaatacaat catcctagat cctgacgggt atttaatctc
     4381 tctgacggaa cctgcttcag atgaatatgc acaaattccc tattatcatg ggtttgctcc
     4441 agcataaccc tttatttatt agatagtatc ttactactat tacataataa tttagatatt
     4501 ataaataaaa attatatata acgattgtaa tattattgtg gcaataattg aacaatagga
     4561 cctaattcaa atttgaatat atctagtaat tattaaagtg aaaatatgat atcaatatgg
     4621 cataaagagc aagaagatat aacaacaaat attattacta tatatagttg tacaatctat
     4681 tttcaattaa attattttga tcatttctta tattatcatt ttttagtata ttatataata
     4741 ttatgttgtt tgtatttttg tactttccta tagataaaaa agattataat ataagaaaat
     4801 cttgttaatt gtactctata aaatattaaa tatcttttat aattgtatat aaatataaat
     4861 gaattgatat tatatgatat aaaatggata atatattaaa aataataatt gtaacttttc
     4921 tagggatatt aatatcacat gtttttacag tagaatttgt taattctcag cctgtatcta
     4981 tggaaggaac atctagtaat gataaaatta tcggaagaga agggaacgac aatattgaag
     5041 gaatggaagg aaatgatgca ttagatggtt ggtttggtga tgatgagata gaaggaggtg
     5101 agggaaatga taacttaaag ggtggtaaag gaaatgataa gttgtacgga gatgaaggta
     5161 atgatattct aaaagggtac ctcggaaatg attcattagt aggtggagaa ggtgatgata
     5221 aattagaggg tggtaaaggt ggagatttac tagagggtgg taacggtgga gatagtttta
     5281 tttgtgattc tgatgatgta atagtagact tcaatcatca ggatggagat tcaatagagg
     5341 ggccttgcag atatgattat attcctaaac aactatattg attttccaat gcatagaaaa
     5401 attatttcct ctatatctac ataataaata aaaaacctaa ctttatatat aataacctta
     5461 gtcttttgta tttctattta taaatattat tttatcaatt ttataaccag gcacttttca
     5521 tatgaccaga tcgatatatt attaattttg tagcttcctt aaatataatg aatttagtga
     5581 taaaaatagt ctctttaact taaatttcaa gttattagat gaattatttt aaataatgat
     5641 attattaatg ttgtagcatt ttaattattt caatctatcc ttttcaaatt ttaatgatgc
     5701 agaattgata caaaatctat tcccagtagg agttgggcca tcgtcaaata catgtcctaa
     5761 atgagcttta cattttttac acataacttc aattctatgt ttaacctact atagtgtatt
     5821 tttactcatt taagttttct aaaagtttct ttaacaacct ggaaagatcg tgtagcaaat
     5881 gcagaagttt tataaatatt ttctgagatt gtttaaatga tatttcttat ggataatatt
     5941 tttttcaaaa tcgtttccaa atttcttgct aattactatc tatttaacca aaataatgaa
     6001 tgttattagc taaaaaatag ttgctgctgt ttatgtttta aattttattt gtgtctacga
     6061 taatttaaat ccttttattt tctttataaa aagattagca ttaacattaa ataaaattaa
     6121 aataagtaat attgtatgag cgatagcaat ataccttcta atattacttc tattactgat
     6181 tttccttttt tagatattcc tgcatcactt gttgaagaga tgttatcaaa aacaaccaaa
     6241 ataagtaatg atctagttaa ttcccttgat acaattagga aaaaacgaca ttattttcgt
     6301 tcacaattgg atagttctga gattttagga aaagatactg aaataaaatc tcacaacatc
     6361 ttagattctt gtggtataga tggagcattt gcaattgaac gtctcttggg aacagatctc
     6421 atagctatag ctactgttgg ggtagaaggt ttaatgtctt cttatcaaaa aggaaaatgg
     6481 gattctccta atcataacgt cttcataaat cccgaacgac acgatcctga aaatatgatt
     6541 gcagccagag gattaatgat agaaatggaa ataaatcttg cagcaaatgc accgtatgat
     6601 ttaatctttt tggacggatc tcttactacg ggacttatcc atatgtataa agctatagat
     6661 tttatcgaaa aacataaaag cgttacagca aatcaaatac gtgataacta taagaatttc
     6721 ttaatatctt acaaaaaaat tttagaatca aaagataata aaaggcaatg gataggaatt
     6781 cctaaatata cttcaagaaa cgatattgga gacatttatg attggcctac agattatgat
     6841 gatagagcta ttctaacttt aattttaaat gaaggagaat atacacttcc aaaaccattt
     6901 acattaaaag aaaattggca taaaaaactc cctactattg ataatggtaa agatgattat
     6961 gaattggaaa atttaatgga gggagttatt gatgagatta aaagattaca tgttatatat
     7021 tataagcctt acaaatggtc tcctgctatt agaattgaga tttcatcttc agtagcaaat
     7081 gatagtaata gattttatgc attacttcag tcaatcaaaa gtcaatgttc tattccctca
     7141 attatggagc cttatcctct ttatctggcc gatcgcatgg ttaaacatat tgcacctgct
     7201 attccagcat acaaacaggt attgacaagg agcatgaccg agattgaaaa aaatgatgaa
     7261 agcatatctg atatcatttt tattatgcat tcatatagaa cagaaagtgg aatgtgatag
     7321 atatgatttc acataatata aacaataacg ataataataa aataaaaaat aatgattcag
     7381 atttgaattg tgatataaaa ttaaatcaac ttttatccaa taccgaacca ataggtacta
     7441 tcggatctcc ctcatcaacg agcgaaataa gtttaaatat attacaaagt gctgtaacta
     7501 aaaaattaat aggagagctt gtaattttta gatatctaca agaatctaaa tcccattatt
     7561 ctttaggaca aattacagag atagaactca aaaatgtact cctagaagat cctactataa
     7621 aatctgttgc tagacaaaga ggtttagtaa atcctataag tggaattcaa gatacgcacg
     7681 taggaaaact gttatgtagt gcagtcttta gcaataacga cgaggagaat ttttttgata
     7741 caagtttatt aggaactgta cctgctacag gaacatatat acatataaca aatgataata
     7801 tactaaattc attactcgaa aaacatttag acaaaatctt ttatttagga tatgtatatg
     7861 gatcacaaac taaattacca atgtggttta aacattttgg aaatggaatt gaaggtgcag
     7921 gggaagcata tcatttaggt atatttggta caaccggttc tggtaagtca actttagcaa
     7981 agatgattct aatggcatat gctaagtatc ctgaaatggg tctttttgta tttgatccag
     8041 taggtgaatt ttctaaagat gcaatgtttg ataatgctaa tggattgaat tatcaatcaa
     8101 aaaataatgg ctttatttta aatattaaag aaatattaaa ttcattagga aagaaatttg
     8161 aaatagtaaa tataagaaac ttaatcttag ataggtggga gttattcgaa gaaatcttat
     8221 atgaatcttc attttttcca aacctgtcca ttaaaggaga aaataaacgc gatgcagtta
     8281 atgttttaat aaatgaaata aaaaataggt tacacataag tctaaccaat cttattgaat
     8341 tggaatcatt ccaacaagta cttaatatat tgcgtaatcc taatattcag ctattaatat
     8401 actctactgc tgaaccaaga gcaagactaa ataccgtcct ttcagaagtt gatgaaaatt
     8461 atttatatga aagattttgg aagcccattt gtcaactatt tcattataga ccaggagcac
     8521 aaactgtaga ctctctacta aaaagattta caaatgatga aaaaccttta gttatagtag
     8581 atctatcatt acaaagtgct catcatctaa aactagatta ttggaatgaa actatacaag
     8641 cattaatttt aaaaagaatt ttgtatggtt tgataaattt aggagaaaaa agttgggaac
     8701 aaaatacttc actaaatact ttagttgtac ttgatgaagc tcatagattt gcaagaagag
     8761 aaaaatatga aaattctgac cttgaaaatc taagacttac tctcttggac gcagtgagaa
     8821 caactagaaa atatggatta gggtggatgt ttataagtac ctcaatttca agtgtacata
     8881 aagatattct tcaacaatta agaataatgt tctgtggttt tggtttatca ataggaagtg
     8941 atttagctac tctaagagaa ttagtatctg atcataatgc acttaaacta tatcaatcat
     9001 ttactgatcc tgcaacttca tttagtacta aatcaagaaa atattctttt atgagccaag
     9061 gaccagtatc tcctctttct tttgctggta ctccgctttt tttaaatgtc tttaattcat
     9121 cacagacatt tgtaaaagaa aataaattac tttcatatac aagaaagagt atatctaata
     9181 gtttaactta tactcaggat aattatgatt tttaattgtt atattatttt catttgtaat
     9241 tttaagattt cattatttaa aattaaattt tgctaaaatg agaataattc atatttctga
     9301 tactcatctt ggaaagaggc caaaaagaac aagagcttca attattaatc aagagataaa
     9361 accaatagaa gatgattttt acaactcttg gagaagattt atagaggaaa taataaatct
     9421 tgataaagat gaaaaacctg atatcataat tcatagtgga gatttttttg acactccgtc
     9481 aggaactgat ccttcaccac ccccagaatt ggcaagaaaa gtagctgcag aggcattcaa
     9541 aaaattatat gaaaataata ttcccattgt tataattgac ggaaatcatg gtcgttatat
     9601 gcaatataga atttcaccat taactgaata cacaattttg tttgataatg tatatctttt
     9661 tacgtatttt gatataagag atagtataag aaatcaacaa cctttattca aagacttccc
     9721 aactttaaat ttaagaattc atgcacatcc atcattagaa tccaatgact taccacaatt
     9781 attattaaaa tatgaagaat gggtctacat acaaaacaat aatattaatc ctgatatgat
     9841 aaacgtattt ttagctcatg gaatgataga aaatcatacc ttaaacagca attttttgat
     9901 gggagattat gattatattg gactgggaga taatcataag atgcaacaaa tcaatgataa
     9961 tgcatggtat gctggatcaa cagaattgtg gagttttaat gaagaaactt atcaaaaagg
    10021 ttatttggtt atagatattg atgattcttc ttctaaatct aaaattaaag tgagtcaaaa
    10081 acttttacct caacagagaa aaataataag tgatcatgta gaaatttatc cagatgataa
    10141 taattctaaa attatcgatc gtattaaaaa ggtatttgag aaaaatggac ttgatacacc
    10201 ttataattac caaaccgctg ctagagttaa aataactata cgaggtaata gaagttatgg
    10261 ttcattttat aatatcaatg aaatatcatc ttatctaaat aaaataacat taaatagcaa
    10321 tgagtataac atagtagaat ttattctaga aacacccaaa tattatgatg atactgttat
    10381 taaacaaatt aaacaagatg atacatttat agaatattta atagaagatc cagagaaaga
    10441 atttaaaaaa tatattacat ctactagaaa agaagatcta aaaaaaaata atttagattc
    10501 aaacgtgctt gcgaaacttt tctttgaatc attaaagaat cagtagtgat attatgaaaa
    10561 ttttgaaaat agaatcaaaa aatttcaaac catttgataa tatttccata ccgtgtgagg
    10621 ggtatcttgg agaaggattc tttattatca aaggaaaaaa ttcaatgggt aaaacaagta
    10681 taatagaatc tattctatgg ggcctacttg gtgaacatct gatggcagaa aaagatagaa
    10741 aatccataat taaaactgga gaatctaaat gtgaagtagc tatcacattt gaaatcagtg
    10801 acattctata tagagtaata agaacaattg aagttattag aaaaagaaag tcattgcagg
    10861 accttcaaaa tgatttggtg aaatcttctg cattgctatt taaaaaagaa aaaaatgaag
    10921 ataaattcat tcagctttgt agaggtccaa atatcgttaa tcaagaaatt gaatcacttt
    10981 tgggagtata tccagaagta attgaaaaaa ctatatatat tagacaaaaa gaagtagata
    11041 aacttgcact cgcagatcca agtgaattaa gagaactaat caagaactta tttggattaa
    11101 atgattttga cgaccatata aaaaaatact taaagaatca aattaatagt attaatgaag
    11161 aaatcaaaga tttaaaaata caaattggaa gtttattaac agaacaaaat gaatgggaaa
    11221 agcaaaaaca aattttagaa gaaaaaaaag aaaaacttga attaaaattg caggagataa
    11281 aaaatgataa aaatacttta attagttatc cttctcaaga gaaattatat aaaattaagg
    11341 taagtataga aaatatagag aaatataaaa aagatgtaga ttatagagaa aaaatgtata
    11401 attataaaaa agaaaatcta ttagtacaag aaaatagaat aaaagatctt caagataaaa
    11461 tatttctctt agattcaaaa attttaaagc tttcaaaaga actacaagaa tatccaccaa
    11521 aagatagtat aagaaaagct agtgatatat taaaaggaat agataatgat gaaaaacaaa
    11581 taagaaattt gatatcaaaa tcaaatcaaa agttaaatat tgatattcaa aatatatcaa
    11641 taaataatgt agaagaactt gaaaaagtta aagaaaaaaa tcaaattgaa ttagaaaaga
    11701 ctgacaaaga aagaatttca atattaacta taatggaggg acataaaaaa ctttttacgt
    11761 ccaatcagct attatatgaa attcagaaca atagcattaa atatatacaa gatagagata
    11821 aatgcccaat atgtagtagt aaaattaacg ataaaaatac attgctattt gatctcaaac
    11881 gagcaagaga aaatatattt aataaagaaa attcatttaa aaaagagata aatcaattag
    11941 aaataaaatt aaagaaaata gaggcagaac ttgacgaaaa aaaaggagta agacaattaa
    12001 tagaagttat agttaccata gtaaatggat taatagaaaa acgtttcaat ttttatgatg
    12061 agttaaattc tatttcacaa gtatataaga aatacgatga actaaataat ttaaattcaa
    12121 ggataattga tgatcttatt tcatcagtat cagagataga aaataatatt ttttcatcaa
    12181 aagaacttgt aaaaaatttt aaagaaaggt tagaatctga aaatattaag cttgaaaaaa
    12241 tgaagaaaga aataaacata ttcaataaag agaaaaatga cattttatta aaacttcagt
    12301 caaataaatc aaatttacaa acatattgtg atgatataaa agtatcaaat attcataact
    12361 tattagcaaa ttttaattgc aatagtattg atgaactaat ttcaaaaaga cttaatatta
    12421 cagtaagaat tgaggaaaaa gctaaaacta ttgaaaatat tcacggggat atttcttatc
    12481 taacagaaga aattgaaata agaggaaaaa aaataatcag tttacgagtt gctgaagaga
    12541 aacttaaaga aaaagagtat ttgttaaaac atgttctatt tttactggga gaagtagatg
    12601 gatttatttc tacagatatc gttgaaggaa gattgtctga tgttttaatg aaaaatacta
    12661 atttttatct aatacctttt actgaaggta gatacgaaat cgctaatata tcatctacaa
    12721 tgagaaaaat aaaagataga atatctcatg gtttaacttt gactttggta gataataaag
    12781 atcacataac aaaaaataaa gaacaactga gtggagggga tgaatcagct cttggtcttg
    12841 cactacggat ttctatatca aaacttatgt cgactatcag acctttcaaa cacgtagaaa
    12901 aaaaagtacc tatgataaat tttattataa tggatgaacc tatgaccagc cttgacgaat
    12961 tccgtagaaa tgtaataatg aatattttga tgaaagatca gagctttaaa cagatctttc
    13021 ttatatctca tgctgaaata aatgaagaag agaataatta tcattctata gtaattaatg
    13081 ataatgaaaa ttctaaaaga atggtagaat acttccatta aaaattttat ttttttaaat
    13141 tatacacaca aatttgttat tgtaaactaa tagactgaaa attgacaatg agtgtaaatt
    13201 aaatggcaat aattgtagta taatataaga acaaaaatga cctctaaaga ggaaccccct
    13261 ccctttaaaa atcatcacaa tttacagaat ttgactatct atttcttttt ttgataatta
    13321 tatacaatat atcaaagttt aatttatccg ttatgaaatt aaatataaaa tgaaactata
    13381 caaaaaatta acaataatta acactatatt aggaataacg gtatgtatta taattttgtt
    13441 aatatacttt tttataaatt cattaattgg tatacctctt ttagggcttt ttttaggggg
    13501 tactattttg catgttttca ttgtattact tattaattta gcaggaatag tttctatatt
    13561 ctatataaaa aatacaagga tcgtgggaat agctttattt atttatggaa ccttattatt
    13621 agctactgct accttttttg gaataccagg atttatttta tttgttatcg ctggtataat
    13681 ggcattaaaa gaaaaacaag taataaaaga atcaaaatga tatcaaatat attacaaaga
    13741 gatgctattc caatcttgtt atataaattc taaaagaata aggaagagag taaatagatt
    13801 gactcaacca ctattaggta caagtaaata gtccatttac agagaataca aatggcaaca
    13861 tatgtagctc tatctcatgt tgtatctctt aattaaaagc ataatctcat tcaaagcagc
    13921 atttggctag tgaaattgcg gaagttgtag tgaattgtct ttatatagaa atgatttaat
    13981 ctattttatt agattaatta attatgatat aatagtttaa tgttatatat ttaattgcct
    14041 ttaccattta ataatatttt tataaaataa aaatcatgac tattttataa ataatgatct
    14101 aatatttgta gaatttctac tatttaattt aattaaaatt tatttaaaat tccacaatca
    14161 tttcttttat atatccatgt tggtaagtga gtggatagtt tacactataa aaatattaaa
    14221 caataaagtt aatattaata ttttctaatt aaactacaat ttagatagtt tttactatta
    14281 atagaatctt taccaataaa tcatgtaaat agcaataaca tacctagata gtattctaaa
    14341 cagtgtgata taattaataa acattgaaat aacagattat aatcattatc aaaaatactt
    14401 tatgaaattt acaaaatctt ctattattta ttttctcctt gtctttgtac ctatttcagt
    14461 tattttagat ttggttcatg ccgatcatat tattatattt attattgcag taatagcatt
    14521 aataccactt gcaaaactca taggtgattc aacagagcat ctttctacac attatggatc
    14581 tactctagga tctcttttaa atgtcacttt tggcaatgca gcagaaataa taattgcagt
    14641 agtagctatt aatgctggtc taatagacct tgtaaaagca tcaattactg gtgcaattct
    14701 tggaaatatt atgcttattt ttggtttgag tatgattgct ggggggattc gtaaaaagga
    14761 acaactattc agtcgtgaaa atgcaggact tcaatcaact atgattttct tagctataat
    14821 tggattggca attcccactg tcttatcaag tactatacta aaacctgctg aaattgaaaa
    14881 tcaacttaaa atacaatttt taagtgatgc cttagcaatt atattattat cagtatacat
    14941 agcaggaatt gtatttacat tttttactca taaacacttg tttgtttctc cacacatgga
    15001 agaaggaaat aacaatcaca atattgacac gaccaccaaa cattgggata agaaaaaagc
    15061 attctttatg ctagctatta gtatggttgg agttgtagtc ataagtgaaa ttttggttgg
    15121 atctgtagaa gaaactagta agcagtttgg ttttggagaa gtatttgttg gagctattat
    15181 aattggaata gttggtaatg ctgcagaaca tagtagtgct attattcttg cgagaaaagg
    15241 taaaattgat ttatccatcg gaatagcggc tggttcagga actcaaatag cactttttgt
    15301 tgtaccactc ttggtaatat ttggaataat tttaaatcag ccatttacat tagaatttac
    15361 aatttacgaa ttagttacat tatttcttgc agctattatt cttaat
//
LOCUS       DASVEV010000154        25059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_831841, whole genome shotgun sequence.
ACCESSION   DASVEV010000154 DASVEV010000000
VERSION     DASVEV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 25059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25059
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831841"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /locus_tag="VF222_07080"
     CDS             complement(<1..1920)
                     /locus_tag="VF222_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7142224.1"
                     /translation="MASDTRSNIHSSHSDVQRIDIVYGIQNIGTTYSQLYLHAEIKID
                     NCINYYNLLSYFELESIKQSIIEAKSRGVVLRYITEITKDNIKYCKELAKIVDELRHL
                     DGLKSNFVTSESQYIAIPVLEKSQLPTQIIYSNINPIVQQMQCVFNTFWYNAISAEQK
                     IKEIEEGEGHYGTKILENQEEIINKIIHLAETSSKLLIVSSYGGMQLIYNNFLTSYKK
                     LLDENKKREGKGIKWICNVEKQTLEIVKIFLDMGMQIRHVQNLPPMSFALGDREINAT
                     IEKMEHGKMVQSLLTSSEPLYVKHFLSIFEDLWNSGIDATNRIIELEEGIELEGIKII
                     RNPVVVQKIAFDLLKSAKEEILIIFSTANAFHRQEKAGSIELLTEIASKKGDVNIKIM
                     TPIDDKLKDIKKELENIETNEKVEDKKEKIVDISREKSYKLQKSNKIQIRFIERQQQT
                     TISILIVDRKYSLAVELKDDTKATTPEAIGLATYSNSKSTVLSYISMFETLWLQTELY
                     DQLKVNDKIQKDFINVAAHELRTPIQPIIAITDIVYSQSKGDKENRELLEIILRNAKR
                     LTRLTDDILDVARIESQSLQTQKEWFNLEDVIASLVSQYKTNIKKMDLTINFNFIREE
                     KKLFIKADKGRITQVL"
     gene            complement(2507..3847)
                     /locus_tag="VF222_07085"
     CDS             complement(2507..3847)
                     /locus_tag="VF222_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX7142225.1"
                     /translation="MDKPRKSRFPSNNINKSIVSLVLLIKSLRPSLISGAADNDPSAI
                     TNYSHAGAKFGFGLLWIVVFLYPFIAVIVGICARIGIATGSGLTSVISKKYSRKVAFL
                     IIALLIIGNIMNISADIGAMAASTALLFPHIPLIGFSILFSFIILLSVILIPYKKYAR
                     ILRYLTLTLLVYFITAIIIGGGNWNEILVSSLVPHIELTPEFMMMIVAIFGNALSPYM
                     LFWQTSEEAEESVASNKQKEMGFGKPVISNREIKLVKVDVALGMAFAVFVMWSIMVTT
                     AGSLHVHNIYEIQTAEQAAQALEPLVKAFPHSGEVSKIIFVIGIIGTGLLAIPVFAGT
                     CGYAVSELFGWKEGLSKQFNQAKQFYLIILICIIIGLIINYINPISPIQMLLYAATIN
                     GIILVPILIIIMRIANDKNILGNGVNGLCSNILGWFIIFIILCAVVTFVFLKFL"
     gene            complement(4552..4770)
                     /locus_tag="VF222_07090"
     CDS             complement(4552..4770)
                     /locus_tag="VF222_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142226.1"
                     /translation="MEVKIDKISLGVGLFFILVSAILLLFDSQTNSITAQPTENKKNE
                     NKNGAADLTTIKTKINLNNTDIKNHKIA"
     gene            5177..5446
                     /locus_tag="VF222_07095"
     CDS             5177..5446
                     /locus_tag="VF222_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7142227.1"
                     /translation="MITKRGTFEMLIPLCCTTKPVRYKQFRVLMKGISSKTMARRLKE
                     LEKMGILKRQAYNEITPRVEYKLTKKGQELVESIINLIQWMIKWS"
     gene            5772..6749
                     /locus_tag="VF222_07100"
     CDS             5772..6749
                     /locus_tag="VF222_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7142228.1"
                     /translation="MKLNGNELPKDSGIEWISNDKLTWINIEKPTREKMKMLELHYPF
                     HELNIEDCLSKIQIPKVDRYGDHVFVILHFPTMENTESFPRSSQLAIFVGYDYLVTIH
                     QGNLRALTDIFQICKINQNQRNSFMGNSSGYLFHSIIDLLVDDLLHILIKVEGNLDDI
                     EDLVFNERIAIAKEISHLRREITTLKRLVYPLKRTILTISREIQKFSEEDLTLYFDDV
                     KDHIDKVIEVLDESKETIEIFKDTDFMLSAEKTNKILAILTILFTLSIPATIVATYYG
                     MNVNLPGGIETGAATFLGPYTSFIIIILISIIPAGAMIYRFKRNGWLEF"
     gene            complement(7162..7626)
                     /locus_tag="VF222_07105"
     CDS             complement(7162..7626)
                     /locus_tag="VF222_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX7142229.1"
                     /translation="MPFNLTDMDVSIIKELLKDGRKSFREISRSTGISTPTVKARFDR
                     LVNVGFIKSVSPVFDFNKIDLRNVNPEIKQSVKREVNSIDQNTYNKIIKKNLEIHLEC
                     DFCKGPVSGKTHVLKFADIERFFCCSNCRTSYKEKYSGRIEAIKKRFKKDDE"
     gene            7713..9965
                     /locus_tag="VF222_07110"
     CDS             7713..9965
                     /locus_tag="VF222_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7142230.1"
                     /translation="MAKDPICGMFVEENETSIHHTKDGITYYFCSTQCLNEFLEPEKA
                     LKKLKIHVTISIALTIPIIILSLPHMIPQIGHVFPIEMMEYTNYILLALATPIQFWIG
                     WRFYRGFWDGVKARASNMDTLIAIGTTAAYLYSAIVTIVPGYFPFTAVYFETAAIIIT
                     LILVGRLLETKTKEKASDAVRKLLDLKPRTARVIRPVIKERNMTEISKKNNNNNNHSI
                     QSNINFDNSNLKSITKEFKEIEIPVEQIAEGDLMVIRPGERVPTDGLVIDGSSSLDES
                     ALTGESIPVDKTKGDEVIGATINKNGLLKVKATKVGQDTVLSQIITLVEQAKTGKAKL
                     ERMVDQVAKYFVPAIVMIAIGVFLGWYFIGGAGLTYSILAFVSVMIIACPCALGLATP
                     AALMMGAGKGAENGILYKGGEHIEIASKVNTIVFDKTGTLTLGKPSVTDIVSLTDIGE
                     QELLRLAAIAESGSEHPLAQAVIRKAKENGTPIANPDSFEAVSGHGLKATYSNHTIMI
                     GNRKMMEDSQIAVTESVDAKLAVFEKEGKTAVLVAIDNTLSGIIAISDTIKEKAKEAI
                     KALRFKGIEIIMLTGDNERTAQAVASKLNINRVIAEVLPHQKEEVISKLKTQEGKVVA
                     MVGDGINDAPALAKADLGIAIGSGTDVAKETGGIILIKDDIRDVVTALDLGKRTVAKI
                     KQNLFWAFAYNTGLIPIAAGALVPILGLGVFGWLPILAGLAMAMSSVTVVTNSLLLGK
                     YKPKYNIPQS"
     gene            complement(10019..10642)
                     /locus_tag="VF222_07115"
     CDS             complement(10019..10642)
                     /locus_tag="VF222_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142231.1"
                     /translation="MIINEKKYFLLSATVTLLFIIQSFHIEEGFGAVNYVPQDLDYGI
                     KFHYPDTWKYLPKYNIDTADDLSTTSFTIIHNSSIINDIDKSFNGYLITIKVIDDIPL
                     PTTTSAQQWARTALTDISEQPNKYKLIEPATPNGEFKTLPISQGKYNGYTFTIGDKTT
                     QKIKVKTVLVLDNKRYEFDYTSSNINNEIAYTYINDYKEILNSVKFY"
     gene            10872..11267
                     /locus_tag="VF222_07120"
     CDS             10872..11267
                     /locus_tag="VF222_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7142232.1"
                     /translation="MTSITYLSSTSVFAIPSGDQQKFTAQLSGDQEVPSIQTNASGMT
                     WFKPKQDRIEFELNVTNLQGITMAHIHNGKQGENGPPVVPLFKSESPTVLINGKLANG
                     NITASMLEGNWQVNNYLTYLLHCQTEPHM"
     gene            complement(11478..12179)
                     /locus_tag="VF222_07125"
     CDS             complement(11478..12179)
                     /locus_tag="VF222_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142233.1"
                     /translation="MKPPKFVIIVIAIAVLAGMLNVYNVNNEEVYAQNLTTNTLHGMT
                     QSANNETSYSSDNNNNSVLDSYQKSQTFIHGIVVKIDNVGYYFDGPADGANGTKDAPG
                     HYWWQDSPTHLYGLHYNIGPFGEESWWSSDAKGNELLYVVDVIIAPWTNTTAYEFAKQ
                     GYVHYHELIRTDNGEKHPELVAWFKHVAVPASFILDKGAMPEDKHIVSAGIDYRFLNN
                     YFMPYNPKVDHGHYE"
     gene            complement(12696..13133)
                     /locus_tag="VF222_07130"
     CDS             complement(12696..13133)
                     /locus_tag="VF222_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142234.1"
                     /translation="MKFNRDVIIQMVIGRKLFDEIDKPIGYKIVYENSFGVESESSWN
                     GIIKGYNGFPDGKNVGSGYSFEGKNRVIVSNWDGVFTTNDGDQLTFKGRDMNQEGKFV
                     VLRTYSTTSKKLSWINGLICLLEGTFNKNDKEFKSIGYEWIFQ"
     gene            13313..13846
                     /locus_tag="VF222_07135"
     CDS             13313..13846
                     /locus_tag="VF222_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX7142235.1"
                     /translation="MANNRYEKKTNSIREKIFQFVNESPGIRYRELLRITGLTNGVLS
                     YHLNFLDNSGKVRVNRVNDRVTRYFSHDVPIDEFNLIGLFRQPTTRKIIIYILESRTC
                     GFNDIVMHTKKVPSTISWHLSKLRDANIIKVRKQNEFKYYEIGINKLKLQELLSKYTS
                     SFTEKIIDDYVDMINEF"
     gene            complement(14201..14827)
                     /locus_tag="VF222_07140"
     CDS             complement(14201..14827)
                     /locus_tag="VF222_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142236.1"
                     /translation="MQLVYGHTEIKVGNYTLEAGWSIEPPLINNLNEIVISVLENDSP
                     VRNAMKDLSISINYGGINKKLNFIPSEESAGLYLADIIPSKLGTYSLTLKGAIGTQSI
                     NNDIQIEEVEDAKKLTFPISEGSSTNLENIGKQITPIMKDLASQIDETKEEINSTKEL
                     IEKMNDDDNSTKSEIERTNLLSYIATALSTSALILIASRGKIKKKRGE"
     gene            complement(15299..16354)
                     /locus_tag="VF222_07145"
     CDS             complement(15299..16354)
                     /locus_tag="VF222_07145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7142237.1"
                     /translation="MILPARNEQKYIEKCLDSLLKQDYSNYEIVVINDSSSDRTGEII
                     QKYSTKNSKIIFINAEAKPEGWTGKNWACYQGYIKSTGQLFLFTDADTTHSSSTISLA
                     VNNLLAKELDALTAIPKILANDFWTKITLPILWTLSISRYSALKANDPKTNVGYFFGS
                     FFIITKKTYEAVGTHKAVKEEIVEDGALGRKVKEEGFKLRVAHGEDHIQAVWARNSST
                     LWHGLRRLMVPLYKREKIKASLMVVATFLLLIYSLIVLPFSIIMALDEKDVTLTINNY
                     SLDLILLFLTIMSILLLIITNVLQLKYTIFQNSLYSLYFPLAGSFVFIAFLSSIINSG
                     KKDVINWRGRRYSIKEN"
     gene            complement(16796..18499)
                     /locus_tag="VF222_07150"
     CDS             complement(16796..18499)
                     /locus_tag="VF222_07150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX7142238.1"
                     /translation="MIKASDLFIRCLESEGVEYIFGLPGEETNDLMFSLLDSQIKFIL
                     VRHEQGAAFMADVYGRLTQKVGVCLSTLGPGATNLITGVASANMDRSPILAITGQTDS
                     NLRHKESHQNMDVVSMFKPITKWRWSIHNADVIAEIVRRAFKISIEEKAGAVHLELPE
                     DIAKCKSDIKPITKPRRTLRSRPHKGLIKNAVKMILSSKMPIILVGNGCIRGSASSYI
                     RKLVERTGIFSLNTFMAKGVISDKSERHLQTIGIKDADHALIALREADLIIAIGYDLV
                     EYSPKNWNANLDKKIIHIDFTPAEVETYYPPDIEIAADIGYTVDAILLELTKEKEKVQ
                     KEKKINNNNNQDSIDVPFFEIPDLFREIKKQITERIEKFRDDFAYPIKPTKLVIDVRN
                     TLDDNDIVISDVGAHKLWIAKTYNTYIPNTCLITNGFCSMGFALPGAIAAQLVRPNQK
                     IVVMCGDGAFLMNVQELETAVRLQLPIIVIIWCDSDFGMISLKQIHEFGRSAFTRFNN
                     PDFVTLAQSFGAIGYYVKSTEEFSKTLEKAKESTSVPVIIAIDVDSSRNQILLNDDSD
                     D"
     gene            complement(18799..19035)
                     /locus_tag="VF222_07155"
     CDS             complement(18799..19035)
                     /locus_tag="VF222_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142239.1"
                     /translation="MSGLSGLVCHPLFHLDVLNVIQHYALYTRTPNFKIPQMIEIKED
                     NIIRLESLQEFKHSSLILEAKDRKILLGIPLLKH"
     gene            complement(19225..20454)
                     /locus_tag="VF222_07160"
     CDS             complement(19225..20454)
                     /locus_tag="VF222_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7142240.1"
                     /translation="MNIFIGFAVMLFVVVSLGVNSNVIPSNIVSAYGIIIETPFEDNL
                     QHNNNNNNSSANDVLVFDYLSSLYAKEQKEKEDKFVILMFDRGYESIFSKAKPILDKF
                     GFKASIFIACDYIENGEGMNWNQVRQLDTEGYDIQSHGLKHTSLADLKSENEITSVIS
                     GGKECLQKNGFSPTVFQAPYNKGGEDPFIVDTIGNYFNLAFTGHSELMFLNCDGWENF
                     GYDEENYHGTTDCGPYFSDGTTTPTNKFAMKEWSHDRSHYKINNEKNQDPHGIEVSYS
                     LYLEFVRVVNSQTKFNEPGKINAIPIVGYHQITKDDDIATSPELFYLEMQYLYDNGFK
                     VISIDDLGYDKYQDRFYVKNVNTFASESPQFQDKGPTWTETIRTQTVADGSESPQFQD
                     KGPTWTETIRTPTWVNP"
     gene            21245..22687
                     /locus_tag="VF222_07165"
     CDS             21245..22687
                     /locus_tag="VF222_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142241.1"
                     /translation="MKKIKETKISSFASTLVYSRINLIAFFISVSVLMLLTLISIGDY
                     HDDYFPRPAYAAKAESNPEGPIIDDPNLVVQPVYQGLKSPTAMAFIATNDILVLEKDE
                     GTVQRIVNGKILPEPLLQVVVDSKDERGMLGIDISTKDMATIGTPPRPSPINVFLFFT
                     EAKLGEFPMGNRVYKYELTDDNTKLANPKLLLNLPALPDDSHVGGVVAVGPDNNVYLT
                     VGDQRPTAFNRIVYPDSHTKAQNYLNGIEPDGRSGILRITQDGGTVGGEGILGDRHPL
                     NKYYAYGIKNSFGLDFDPVTGNLWDTENGPSFGDEINLVKPGFNSGWANIQGFSTLDD
                     EWNKGEEVVTPLNPKDYNLIDFGGKGKYSSPEFTWDVAPTALQFLNSDKLGKQYENDM
                     FVGDIKNGNLYHFELDQQRTGLLLQGPLADKDASKDEINQAVFAQGFGGITDIDVGPD
                     GYLYVLTFGEEDGTIYKIVPAANGEKKIVS"
     gene            22710..23738
                     /locus_tag="VF222_07170"
     CDS             22710..23738
                     /locus_tag="VF222_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142242.1"
                     /translation="MAILRSEILTVSLIILSLIIMLITPSISIEKSGIAIKNHYAYSS
                     PPPSPSYSSTAAVTLGDFNFAAAGDWGCTSNTIDTVQNIIDQDPEFVLAVGDLSYDSS
                     AKCWFEIISPIENKTMIAIGNHDTDPSMLKEYMDFFGLKGQYYSFNYQNVHFIVMSTE
                     LPYEDGSEQYNFVNNDLSKVSLNPDIDWIVVDYHSLAYTSPANIGKGNSAEKELRDIY
                     HPLFIKYNVDLVLQAHNHNYQRSYPIIYNNTNSENPIITDTNNNNNYYDPKGMIFGTV
                     GTGGESLYPLTGQAPYVATQYVGFGFLNVDVINDGTTLSGKFFANDGTIKDQFTIIKS
                     VNISENTK"
     gene            complement(24054..24434)
                     /locus_tag="VF222_07175"
     CDS             complement(24054..24434)
                     /locus_tag="VF222_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142243.1"
                     /translation="MSTIVHPDDITKAKVVNMILNKKTEEALKNLSKFYKVIPPEITV
                     GTIKGKRKTVYAVYVQRESKIYCINSDVFYNPFIILHEFYHHIRTKGGIHRGSEKYAN
                     TYAKSFIDSYKKIIDQITTQKQQQ"
BASE COUNT         8692 a         3757 c         3716 g         8894 t
ORIGIN      
        1 taaaacctga gttattcttc ctttatctgc tttgataaac aatttttttt cttctctaat
       61 aaaattgaaa tttatagtaa ggtccatttt ttttatatta gttttatatt gtgatacaag
      121 acttgcaatt acatcttcaa gattaaacca ttctttttgt gtttgtaacg attgactttc
      181 tattcttgcg acatccaaaa tatcatcagt aagtcttgta agtctttttg catttctcag
      241 aataatttct agcaattctc gattttcttt atctccttta ctttgggaat agactatatc
      301 ggttattgca attataggtt gtatgggtgt tcttaattca tgagctgcta catttatgaa
      361 gtccttttgt attttgtcat tcacttttaa ttggtcatat agttctgttt gcagccatag
      421 agtttcaaac atagagatat aagataatac tgttgatttg ctattagaat aagttgctaa
      481 tcctattgct tcaggagttg tagctttagt atcatccttt aattcaacag ctaaggagta
      541 ttttctatca actatcagaa ttgaaattgt tgtttgttgt tgcctttcaa taaatctaat
      601 ctgtatttta ttggattttt gtagtttgta acttttttct cttgatatat ctacgatttt
      661 ttctttttta tcttctactt tttcatttgt ttctatattt tcaagttctt tttttatatc
      721 ttttaattta tcgtcaattg gtgtcataat tttgatattt acatcacctt tcttggatgc
      781 aatttcagtt aatagttcta tagagcctgc tttttcttga cgatgaaatg cattagcagt
      841 agaaaatatt attaatattt cttctttagc tgattttaaa agatcaaagg caattttctg
      901 aactacaaca ggatttctta taatttttat accttctaat tcgatacctt cttccaattc
      961 tattattcta tttgtcgcat ctattccact attccataaa tcttcaaaga ttgaaagaaa
     1021 atgcttaaca tatagaggtt cagaacttgt taaaagactt tgaaccatct taccatgttc
     1081 catcttttct atagtagcat taatctctct gtcacctaag gcaaaactca tcgggggtag
     1141 attttgtaca tgacgaattt gcattcccat atctagaaat atcttaacta tctcgagagt
     1201 ttgtttttct acattacata tccattttat tcctttacct tctctttttt tattttcgtc
     1261 aagtaatttt ttatatgaag ttaaaaaatt attatatatt aactgcatcc ctccatatga
     1321 ggaaacaatt aacaatttag aggatgtttc agctaaatga ataattttat ttataatttc
     1381 ctcttgattt tctaatattt tggttccata atgtccctca ccttcttcta tttcttttat
     1441 tttctgttct gcagaaatgg cattatacca aaatgtatta aaaacacatt gcatttgttg
     1501 tacaatcgga tttatattgc tatagatgat ttgtgttggc agttgtgatt tctctaaaac
     1561 gggaatggca atatattggg attcacttgt aacaaaattg ctttttaatc catctaaatg
     1621 tctaagctca tcaacaattt tagcgagttc tttgcaatat ttgatattat ctttagtaat
     1681 ttctgtaatg tatcttaata ccactcccct cgactttgct tcaataattg attgttttat
     1741 tgattcaagc tcaaagtatg agagtagatt ataatagttt atacaattat caatctttat
     1801 ctctgcatga agatataatt gtgaatatgt agtacctata ttttgaattc catatactat
     1861 atctattctt tgaacatctg aatgtgatga atgtatattt gaccttgtat ctgatgccaa
     1921 agatatatag ttatattaac tttataaata taaagtagta tggtaaggag taattaataa
     1981 cattttatta ttttcctaaa aggttccaaa aaattcttca ttatctaaag taatagaagt
     2041 gataggttta ttagttgatt ttatacatcg taatgaaata tttaacaacc accaactata
     2101 tcaataatta tacaaaataa tagtattaca cacaatagta aaaaaagaat attagtagtt
     2161 aatgatgaaa gggatattgg tttaacacta aagatacttt tagaaaaaaa tggttttatg
     2221 atagattagg attataatcc tatcaaggcg ttagaagaat tcaagccgat tttatgattt
     2281 attatttatt gatataaaac tgcccatcat gaatggatat catttataca ttgaaataag
     2341 taaaaaataa tgaaaccaca gatatgctca tgacagcaag aaatgcttaa cgatataaaa
     2401 acttcgataa tatctccttt gttatttatt gattattttt actctaataa ttctgttagt
     2461 gataatatat ttttaatatt aaattaaaca tcatagatta attgaattat aaaaatttta
     2521 aaaatacgaa tgttaccact gcacataata ttataaaaat aataaaccat cctagaatat
     2581 tagagcataa tccattaact ccatttccta gtatattctt atcattagca attctcatta
     2641 ttattattag aatgggaact aaaataattc cattaatagt agcagcatat aataacattt
     2701 gaattggact tattggattt atataattaa ttatcaagcc tataattata caaatgagta
     2761 taattagata gaactgtttt gcctgattaa attgtttact taacccttct ttccatccaa
     2821 ataattctga tacagcataa ccacatgttc ctgcaaaaac agggatggct agtaaacctg
     2881 ttccaattat acctattaca aaaattattt ttgatacttc gccagaatga ggaaacgctt
     2941 taactaatgg ttctaatgct tgtgctgcct gctctgctgt ttgtatttca tatatattat
     3001 gaacatgaag acttcctgct gtcgttacca tgattgacca cataacaaat actgcaaacg
     3061 ccataccaag cgcaacatca acttttacta gtttgatttc cctattagat ataactggct
     3121 ttccaaatcc catttctttc tgtttattgc ttgcaacact ttcttctgct tcttcagatg
     3181 tttgccagaa taacatgtaa ggcgacaaag cattgccaaa aatggcgaca atcatcatca
     3241 taaattctgg tgttagttca atatgtggca ccaaagatga tactaatatt tcattccaat
     3301 ttcctcctcc tataattatt gcagttataa aatagacaag taaagttaat gttagatatc
     3361 taagtattct agcatatttt ttataaggaa taagaatcac acttaataaa attataaatg
     3421 aaaacaatat tgagaaacca attagaggga tatgtggaaa taaaagtgca gtcgaagcag
     3481 ccattgctcc aatatctgcg ctaatattca ttatatttcc aatgataagt aaagcaataa
     3541 ttaaaaaagc aacttttctt gaatattttt tagatattac tgatgtcaat ccacttccag
     3601 ttgctatacc aattcttgca cagattccaa ctatgactgc aataaacgga tagagaaata
     3661 ctactatcca aagaagacca aaaccaaatt ttgcacctgc atgagaataa tttgttatag
     3721 cagaaggatc gttatctgct gctccagaaa tcaggcttgg tcgtaatgat ttgataagta
     3781 acaccaagga aactattgat ttattaatat tatttgatgg aaatctagac tttctgggct
     3841 tatccactac ttcatactag aatccaacta gtttattctt tattaaaata aagaatattg
     3901 tgtatcctga ttactaattt tcagcggatt taatattaaa aaataactgc tgcttcaatt
     3961 taatagaagt aaatattgca attgctacaa gtcgagagga aggttaatag tactacaaat
     4021 ttaacaacat agaatttttt aatatatcat ttaaatatac tttcacttca aaatttacat
     4081 tgatgtttta tattctaaat ataacttcgt aactcacaaa cttaaattcc atagattaag
     4141 agaccaagaa tcgtgtcaaa tttaattcac gaaccatgag ataaatacaa aaatgttgga
     4201 aaccttctta gcataataca atataataaa ttagtctcat aacttatttt tgatagaatc
     4261 ataagcaaag tttaactgta tctttattag tagaagataa acctatgttt ccttcattac
     4321 tatcatacaa tggaaggtga gagttagcag cagcagcatt cagtatatca tttaatacat
     4381 atccgtatac atactattca ttatcaaaca tcacttgaga aatatcatta cttttatgga
     4441 attttaaatc aatggttaag aaattatcgt ttaaatttat cttattttta ttttctttta
     4501 gatcaacata cttagcctgt ctgtccttca ccattaagat aaccaattat tttaagcgat
     4561 cttgtggttt ttaatatctg tattatttaa atttattttt gttttgatag tagtaagatc
     4621 agcagcacca ttcttgtttt cattcttttt attttcagtg ggttgagcag ttatagaatt
     4681 agtctgacta tcaaataata gtaatatagc tgatactaaa ataaaaaata atccaactcc
     4741 taaagaaatt ttatctatct ttacttccaa tatgatattt caaatactct ccaataatta
     4801 ttataaatat cacgattaat ttcattagag agataagtga ataaaattta acttttgcta
     4861 tttgactttt gcgatttgaa tatttacttt tattaaaatc tcctaattgt agtcgtaaaa
     4921 aaataaatta agagaattat tctcttctaa tttgatcttt aaatattttt tttattttct
     4981 tattttggta ttttttttct atgaataacc taatgtttac aaagtaaatt atttaaatta
     5041 taagacatat atacacggta gagttatggc tgaaacaaag atacaaaaat ggaaaaattt
     5101 accattttat gataattgca gttttgaagg ttataattca gaagaactaa tgcaagatac
     5161 agcataactg tgagatttga ttacaaaaag aggaacattt gaaatgctta ttccgttatg
     5221 ttgcacaaca aagcctgtta ggtataaaca atttcgagta ttaatgaaag gtattagtag
     5281 taaaacaatg gcaagaagac ttaaggaact tgagaaaatg ggaatattaa aaagacaagc
     5341 ttataatgag ataactccaa gagtagaata taaattgacc aaaaaaggtc aagaattagt
     5401 tgaatcaatt ataaatttaa ttcaatggat gataaaatgg tcttaataca acaaaatgaa
     5461 atcattattt acctctaata agaaaaacaa atgagattct gattttattt taactttaaa
     5521 atatatcaca tcactaaatt tatgatgttc atgccacctc tacaatgtat aaattgtatc
     5581 aagagacaga tttgaatatc ttgatttaaa aaaatgaaaa tcgttactga aaaatagtta
     5641 ttagataatt caacttaaaa ttttacaaca aatatggaca ttaactctat ataagttaat
     5701 ctttattcaa tattatgaat attgttaata tatatagagc atccttgact aattagcagg
     5761 tattatttat catgaaattg aatggaaatg aattgccaaa agactcagga atagaatgga
     5821 taagtaatga taaattaact tggatcaata tagaaaaacc aactcgtgaa aaaatgaaga
     5881 tgctcgaact ccattatcct tttcatgaac ttaatatcga agattgtcta tcgaaaattc
     5941 aaattcccaa agtagatcgt tatggtgatc atgtatttgt catactccac tttcctacaa
     6001 tggaaaatac ggaaagtttc ccacgttcat ctcaacttgc aatatttgta ggttatgatt
     6061 atttagttac aattcatcaa ggaaatttaa gagctttaac agatatattc caaatctgta
     6121 aaatcaacca aaatcaacgt aattcattta tgggaaattc ttctggatat ctatttcaca
     6181 gtattataga tctattagta gatgatttgc ttcatatttt aataaaagta gaaggtaatt
     6241 tagatgatat agaagattta gtgttcaatg agaggattgc tattgcaaaa gagatatctc
     6301 atctaagaag ggaaataacc actcttaaaa gattagtata tcctctaaag cgtacaatat
     6361 tgactattag tagggaaatc caaaagttct ctgaagaaga tctcactctt tatttcgatg
     6421 atgtaaaaga tcatattgat aaggtaattg aagtccttga cgaatcaaaa gagaccatag
     6481 aaatattcaa agataccgac ttcatgctta gcgctgaaaa gactaacaaa atcttggcaa
     6541 ttctaactat acttttcaca ttatccattc ctgcaacaat agtggcaaca tattatggaa
     6601 tgaacgtaaa tttacctgga ggcattgaaa caggtgctgc aacatttcta ggaccttaca
     6661 caagttttat aataataatc cttatatcga taatacctgc aggagctatg atatatcggt
     6721 ttaaaaggaa tggttggctt gagttctaat ttacccatag attacaggta taaaactatt
     6781 gatttagttt tattgaacag tataaattag aattataaaa acctgtaaat agatttcaac
     6841 tttaaattgt ttcaattata tcaatttggc tacatataac tttaacctga tttttaattt
     6901 tatgtatatt tattaatgtt ttacaattgt aaagcttacc acttatattt tcattctccc
     6961 ttataattat aagtaatggt aaaagatcta atttttggaa tgggacgtag atggaaaaag
     7021 accaaacata tttcagatgt aaaccgagga aaagtatatt gtgtcttcac attgcaaaga
     7081 taaatttgac aatgactcta aaatatatgg attttgatta attaattaat taattcaatg
     7141 ctacaagttc tgttaaatag actattcatc atctttctta aacctttttt ttattgcttc
     7201 tatacgtcca ctatattttt ccttgtatga tgttctacaa ttagaacaac aaaaaaatct
     7261 ttcaatatct gcaaacttga gaacgtgagt tttccctgat actgggcctt tacaaaaatc
     7321 gcattctaag tgaatttcta gattcttttt tattatttta ttatatgtat tctgatctat
     7381 tgaattgact tctcttttta cagattgttt tatttcagga ttgacattcc tcagatctat
     7441 tttattgaaa tcaaaaacag gtgatacaga tttgataaat ccaacattta ctagcctatc
     7501 aaatcttgct ttaacggtag gagtgctaat tcctgtagag cgtgatattt cgcgaaagga
     7561 tttacgtcca tcttttaata attctttgat tatagaaaca tccatatctg taagattaaa
     7621 tggcattgta tgcctagaat aatcttttaa tatttcaagt tttacaaatg taaaggttag
     7681 tccttataca tgtatttgta tattactcta acatggcaaa agacccaata tgtggaatgt
     7741 ttgttgaaga aaatgaaact tctatacatc atactaaaga tggaataaca tactattttt
     7801 gctcaactca atgtttaaat gagtttttag aaccagaaaa ggcattaaaa aaattaaaga
     7861 tacatgttac tataagtatt gcacttacca taccaataat tatacttagt ttgcctcaca
     7921 tgattcccca gataggacat gtctttccta tcgagatgat ggaatacaca aattacatac
     7981 ttttggcttt agcaactcca atacaattct ggataggatg gcgcttttat agaggatttt
     8041 gggatggagt taaagctaga gcatctaaca tggatacact tatagctata ggaactacag
     8101 ctgcctatct ttacagtgca attgtgacta ttgttccagg ttattttccc ttcactgcag
     8161 tatattttga gacagctgca ataataatta cactcattct agttggaaga ctacttgaaa
     8221 caaaaacaaa ggaaaaggct tcagatgctg taagaaaatt acttgattta aaaccaagga
     8281 cggctcgagt aataagacct gtaataaaag aaagaaatat gacagaaata agcaaaaaaa
     8341 ataataataa caataaccat agtattcaaa gtaacataaa ttttgacaat tctaacttaa
     8401 aatcaataac aaaggaattt aaagaaatag aaatcccagt agagcaaata gcagaaggcg
     8461 atttaatggt tattagacct ggtgaacgag ttccaacaga tggtttagtt attgacggaa
     8521 gttcctctct tgacgaatca gctttaacag gtgaaagtat tccagtagat aagacaaaag
     8581 gagacgaggt aataggtgcc acaattaaca agaatggtct tcttaaagtc aaagcaacaa
     8641 aggtaggaca agatacggtc ttatctcaaa taataacatt agtggaacaa gcaaaaacag
     8701 gaaaggcaaa attggaaaga atggtagacc aggtagcgaa atattttgtt cctgctattg
     8761 taatgatagc aataggagta tttttgggat ggtattttat aggaggtgca ggattaacct
     8821 attcaatcct tgcatttgta tctgtaatga tcattgcctg tccttgtgca ttaggtcttg
     8881 ctactcctgc tgcattgatg atgggtgcag gaaaaggtgc agagaatgga atattgtata
     8941 agggagggga acatatagag atagcaagta aagttaatac cattgttttt gataaaacag
     9001 gaactttaac tctaggaaaa ccatctgtta cggacatagt atctttaaca gacattggag
     9061 aacaagaatt actaagactt gcagcaatcg cagaatctgg ttcagaacat cctttagcac
     9121 aggcagtaat taggaaagct aaagaaaatg gtactccaat tgctaaccca gattcatttg
     9181 aagcagtttc tggacatggc cttaaggcaa catattccaa tcataccatt atgattggca
     9241 atagaaagat gatggaagat agccagattg cagttactga aagtgtagat gcaaaacttg
     9301 ctgtatttga aaaagaaggt aagacggcag ttttggttgc catagataat actctttcag
     9361 gtataatagc tatatcagat acaataaaag aaaaagccaa agaagcaatt aaagctttga
     9421 ggttcaaagg aatagaaata ataatgctta ctggagataa tgagagaact gcacaagcag
     9481 tagcatccaa gcttaatatt aacagagtaa ttgcagaggt acttcctcat caaaaggaag
     9541 aagtaatttc taaattaaag acacaagaag gtaaagttgt tgcaatggtt ggtgatggta
     9601 ttaacgatgc acctgcacta gcaaaagctg atttaggtat tgcaataggt tctggaacgg
     9661 atgttgcaaa agagacaggg ggaattattt taattaaaga tgatataaga gatgtagtaa
     9721 ctgcattaga tttaggtaaa agaacggttg caaagataaa acagaatttg ttctgggcat
     9781 ttgcatataa tactggatta atacccatag cagcgggagc attagtgcct attctgggat
     9841 taggtgtatt tggttggctt ccaatactgg caggtttagc aatggctatg agttctgtaa
     9901 ctgtagtaac aaactcgtta ctacttggaa aatataaacc aaaatataat atccctcaat
     9961 cttaaatttt ttgtaaaaaa atagaacggt gaaattaaca gttccgcatt gttaactatc
    10021 agtaaaattt aactgaatta agaatttctt tgtaatcatt aatatatgta taagcaattt
    10081 cattatttat gtttgaagaa gtatagtcaa attcatatct tttattgtct aaaactaata
    10141 cggtttttac ttttattttc tgcgttgttt tatctcctat tgtaaaagta taaccattgt
    10201 attttccctg tgaaattggt aatgtcttga attctccatt tggagtagca ggttctatta
    10261 gtttgtattt attaggttgt tcagaaatat cagtaagtgc agttcgtgcc cattgttgtg
    10321 cactagtagt agtaggaagt ggtatatcat caatgacttt tattgttata agatatccat
    10381 tgaatgattt gtcaatgtca ttgattattg aactattgtg tattattgta aatgatgttg
    10441 tactcaaatc atcagctgta tcaatattat atttaggtaa atatttccat gtatcaggat
    10501 agtgaaattt tattccatag tctaaatctt gaggaacata atttaccgca ccaaatcctt
    10561 cttctatatg aaaagattgt ataataaaaa gtaaagtaac tgttgctgaa agtaagaaat
    10621 atttcttttc gtttattatc attggattca agtaaaacta tagacttata actttataaa
    10681 ttgagcaact acattaagat attaattgaa taataactta ttaaataatt aattctaatt
    10741 atttccaaat ttcttcacct gatttatatc cagttgttaa atagtttaag gtaatttata
    10801 ttataataaa attaaataac aatataagag gaatatttgt tattgtaatc tctttatcat
    10861 taataacagc attgacatct ataacatatt tatcatctac atctgttttc gcaattccaa
    10921 gtggcgatca acaaaagttt actgctcaat tatctggaga ccaagaagtt ccatctattc
    10981 aaactaatgc atctggtatg acatggttta aaccaaagca agataggata gagtttgaac
    11041 ttaatgtaac taatttacaa ggaataacaa tggctcacat acataatgga aaacaaggag
    11101 aaaatggacc acctgtagta ccattattta aatctgaaag tccaacagta ctaataaatg
    11161 gtaaattagc aaatggaaat attactgcat ctatgttaga aggaaactgg caggtaaaca
    11221 attatctgac ctatctattg cattgtcaaa cggaaccaca tatgtaaatg tacataccga
    11281 gcaaaatcca aatggagaaa tccgtgggca aattcaccta tggtaaaaaa taaatttagt
    11341 ttactttaga aattaaatta aatatatatg tatatatgca catattaatt agaaacaata
    11401 aaagaattca aagattattt aattatttaa tattaaaaaa ttaaaaaaag ggtaaaagat
    11461 cccttctgtg tattatttta ttcataatga ccatggtcta cttttggatt atatggcata
    11521 aaataattgt ttaagaatct gtaatcaatt cctgcagaga ctatgtgttt atcttctggc
    11581 atagcgccct tatccagtat aaatgaggca ggtactgcta catgtttgaa ccaagcaact
    11641 aattcgggat gtttttctcc attatcagtt cttattaatt catgatagtg gacatatcct
    11701 tgtttagcaa attcatacgc agttgtattt gtccatggtg ctattataac atcaactaca
    11761 tatagtaatt cattaccttt tgcatccgat gaccaccaac tttcttctcc aaatggacct
    11821 atattgtagt gtaatccata cagatgagta ggagaatctt gccaccaata atgaccaggt
    11881 gcatcttttg tcccatttgc accgtctgca gggccatcaa aataatatcc tacgttatca
    11941 attttgacta ctattccatg gataaaagtt tgtgattttt gataactatc taatacagaa
    12001 ttattgttat tatcagatga ataacttgtt tcattattag cagattgagt cattccatgc
    12061 aaagtatttg tagttaaatt ttgagcgtat acttcttcat tattaacatt gtaaacattt
    12121 aacattcctg ccagaactgc aatagcaatt actataatca cgaatttagg tggtttcata
    12181 taatatacaa ctcaacaatt cctataaata agcctatttg ttaatacata gactggaaaa
    12241 taatattatt taaaaagatt aagttaattc gttaattaga gtcgaaatat ttaaaatcat
    12301 tctttgaaaa agatctgatt agtcaataat tataattaaa caaatggaaa ttgtattaaa
    12361 gtaaaaaata agaataattc aatctttaga tttcgaaatt attcaaatct tttagttagt
    12421 attaaaaatt gaagtaagtt ggaatgggga aaaagaagaa ttgataacta tgatattcct
    12481 actcctttac gctattcttt ggtactttta taaaaaacac atacatatac ttcttagatg
    12541 ggattagatt caaacttatc aagtgcaaaa gattctgttg agaactaccg caaggcaagt
    12601 ttgttctaat agtgaaatat tttatgaaat aggatgaaaa agtaatatta ataatatata
    12661 ttatatgcac aatgatgatt taataaaaaa aactattatt gaaatatcca ttcgtaacct
    12721 atacttttaa attctttatc atttttattg aatgttcctt ccaatagaca tattaatcca
    12781 tttatccaag atagtttttt agaagtagta gaatatgttc gaagaactac aaattttcct
    12841 tcctgattca tatctctccc tttaaatgtt aattgatctc cgtcattggt agtaaataca
    12901 ccatcccaat ttgaaacaat tactctattt tttccttcaa atgaatagcc tgaacctaca
    12961 ttttttccgt caggaaaacc attgtaacct tttattatcc cattccaact cgattcactt
    13021 tctactccaa aagaattttc atagactatt ttatatccaa taggtttatc aatttcatca
    13081 aacaattttc tccctataac catttgaata atcacatcac ggttaaattt caatattttg
    13141 aattgtacta gttattgaat tatatcaaca actataatct ttgtatgcat ataactgata
    13201 atcctttttt aaggttattc ctcacgttaa tatgaggttt ctcatctaat cttgttaata
    13261 atgattatat tttttgacat ttctttccta aaaagtcccc ggtgaattcg atatggctaa
    13321 taatagatat gaaaaaaaaa ctaattcaat tagagaaaaa atatttcagt ttgtcaatga
    13381 gtctccagga ataagataca gagaactttt aagaattaca ggactcacca acggtgtttt
    13441 atcttatcat ttaaatttct tggataattc aggaaaggtt agggtaaatc gagttaatga
    13501 tagagtaacc agatattttt ctcatgatgt tcccattgat gagttcaatt tgataggact
    13561 tttccgtcag ccgactacta gaaaaattat tatctatata ttagaatcta gaacttgtgg
    13621 attcaatgat atagtaatgc atacaaagaa agtaccttca acgatttcat ggcatttgtc
    13681 aaaattacgt gatgcaaata ttataaaggt acgtaaacaa aatgaattta aatattacga
    13741 gattggaata aacaaattaa aacttcaaga acttttatcc aaatatacaa gtagttttac
    13801 agaaaagatt attgatgatt atgtagatat gattaatgaa ttctaatatc ttggaatcgt
    13861 acttttgtat atctaatttc atttaactag aaaaggtaag ttacaactta aaataccccc
    13921 catcaataat ggaaatggtt taacaataac catgatccta ataatcatac aataaaaata
    13981 tgtctttaaa ttaacctatt ttttgtcata atttattttg tcatcaatca atccttagat
    14041 aatgcatctg caattcattt gacttgtcaa ccattcaaaa tgttcatctg tttgcttgtt
    14101 attttgcact tattcttatt acattatgag taattgaagt tattcaatta taaaaatgaa
    14161 ttcaaatcta acaaacaaca cttttcaaaa tggtgagaaa ttactcacca cgtttttttt
    14221 ttattttccc tctgcttgct attagtatta gagcagaggt acttaacgct gtagcaatat
    14281 agcttaaaag attagttctt tcaatctcag attttgtgga attatcatca tcattcattt
    14341 tttctattag ttcttttgtt gaattgattt cttcttttgt ttcatctatt tgactagcca
    14401 aatctttcat tattggagta atttgttttc caatattctc caaattagta gaagaacctt
    14461 cagaaatagg gaaagttaat ttttttgcat cttccacttc ttcaatttga atgtcattgt
    14521 ttatgctctg ggttcctatc gctcctttta gagtaagaga ataagtccct aacttggatg
    14581 gaattatatc ggccaaataa agaccagcac tttcttcaga tggaataaaa tttaatttct
    14641 tgttaatacc accataattg atacttatag agagatcctt catagcattt ctaactggag
    14701 aatcattttc caatactgat atcacaatct catttaaatt atttattaga ggtggttcaa
    14761 ttgaccaccc agcctctagt gtgtaatttc caactttaat ttcagtatgt ccataaacca
    14821 attgcatact aaaaccaacc gaatagaaaa atagaagaat gaaagatatt agtataagag
    14881 gatttctatg tatcatggtt atgattattt aaactcgtta ttttaaccta ttaattcaat
    14941 aaaatgttct atcataattt catattgtat acgtctatac tcatttcata tttatgtata
    15001 agatatacac aaaattgtaa gcataataag atttggttat gtatatctca ttaacaaaat
    15061 tataaaaccg taaaaaacag agcaggaaaa agtaatttca attaatagtt tagaaaacaa
    15121 taagaaaatt ttaatttaaa taattcttat gctaataata aattgaatgt cgactgaact
    15181 accactggat aatcattatt gatattatat atacatcaaa acaatacaga attagatact
    15241 aaaaatcaaa gtattttgat tactaaaact aatgacaact atgccaattg tttcattttt
    15301 aattttcctt aatagagtat cttctacccc tccaatttat aacatctttc tttcctgaat
    15361 ttataattga tgaaagaaat gcgataaata caaacgaacc agctaaagga aaatataatg
    15421 aatataaaga attttgaaat attgtgtact ttaactgtaa aacattggtt attattaata
    15481 ataaaatgga cataatagtt agaaacaata gaattagatc taatgaatag ttatttattg
    15541 tcagggttac atctttttca tctaatgcca taataattga aaatggcaat acaattaaag
    15601 aatatattag taaaagaaat gttgcaacaa ccatcagaga tgcttttatt ttctcccttt
    15661 tataaagagg aaccataagt ctacgtaggc catgccatag agtagaagaa tttcttgccc
    15721 ataccgcttg tatatgatct tcaccgtgag ctactctaag tttaaagcct tcttctttga
    15781 cttttcgtcc taatgcccca tcctctacaa tttcttcttt tactgcctta tgagttccaa
    15841 ctgcttcata tgttttcttt gtaattataa agaaacttcc aaagaaatat cctacattag
    15901 ttttgggatc atttgctttt aatgccgaat atcgtgatat tgaaagagtc caaagtatag
    15961 gtaaggtaat ttttgtccaa aaatcattag ctaatatttt gggtattgca gtgagagcat
    16021 caagttcttt agctagtaaa ttgtttactg ctaaagagat agtggaggaa gaatgagtag
    16081 tgtcggcatc agtaaaaagg aaaagttgac cggttgattt aatgtatcct tggtaacaag
    16141 cccaattctt tcctgtccaa ccttctggct ttgcttctgc attaataaaa ataattttgg
    16201 aattctttgt gctatatttt tggattattt ctcctgttct atcggatgaa gaatcattaa
    16261 taacaactat ttcataattg gaatagtctt gtttgagtaa gctatctagg catttttcaa
    16321 tgtatttctg ctcatttctt gcgggaagta tcacacttac tttgggaaaa acaatattct
    16381 cacaagattt taaaaataaa agttttggta ctccttttaa tgatttaata gtataaatca
    16441 gaaagtagat ccaactacta aagataatta cttgaataat aaatagaata attaatattg
    16501 tattatggaa aaccaatcat gcatcatgtg tatatcagtt gactagaata atagagtatt
    16561 tattataata ttacttcttg tatttttaca acattagata tcttgattaa caatagaatt
    16621 gattttattt ttttttgaat attatataat gttgaatgtt atttctaatt ttcataaaat
    16681 tctattaata tattgttaca tatcaaagtg actcgtattt aattttcatt acacaattct
    16741 ttctttcttt gttcttaatc tacgataata gggattataa taataaagat aacagctaat
    16801 catcagagtc atcattcaac aaaatttggt ttctagaaga atctacgtca attgctatta
    16861 taactgggac agatgttgat tcttttgctt tttccaaagt tttagaaaac tcttcggttg
    16921 atttaacata atatccaatg gctccaaaac tttgagcaag tgtaacaaag tctggattat
    16981 tgaatcgagt aaatgcactc cttccaaatt catgaatttg tttaagagaa atcataccaa
    17041 aatcagagtc acaccagata atgactatta ttggtaattg taaacgaaca gcagtttcca
    17101 attcctgaac attcatcaaa aaggcaccgt caccacacat gacaacaatt ttttgatttg
    17161 gtctgacaag ttgagctgcg atagcacctg gaagggcaaa acccatggaa caaaaaccat
    17221 tagtgatgag acaggtattt ggaatataag tattgtaagt ttttgctatc cataatttat
    17281 gggcccccac atcggatata acaatatcat tgtcatccaa agtatttctc acatcaatta
    17341 ctaacttggt aggttttatc ggataagcaa aatcatctct gaatttttca attctttctg
    17401 taatttgttt cttgatttct ctgaaaagat ctggtatttc aaaaaatggt acgtctatag
    17461 aatcttgatt gttattgttg tttatttttt tctctttttg tactttttct ttttctttag
    17521 tcagttcaag taaaatcgca tctacggtgt aaccgatatc tgcagcaatc tcaatgtctg
    17581 ggggataata tgtttctact tcagctggag taaaatcaat gtgaataatt ttcttgtcta
    17641 aatttgcatt ccaatttttt gggctgtatt ctactagatc atatcctata gcaataatta
    17701 aatcagcttc tctcaatgca atgagggcat gatctgcatc tttgattcct attgtttgta
    17761 gatgtctttc agatttatct gatattacac cttttgccat gaatgtgttc agtgaaaata
    17821 tcccggttct ttcaactaac tttcttatgt aggagctagc gctccctcgt atacagccgt
    17881 ttcctaccaa aattattggc attttagaac tcaatatcat tttaactgca ttttttatca
    17941 aacccttatg tggtcttgat cttaatgttc gacgaggttt tgttattggt ttaatatcgg
    18001 atttgcattt ggctatatcc tctggcagtt ctagatgtac tgctccagct ttttcctcga
    18061 ttgaaatttt gaaagctctt cttactatct ctgcaataac gtcagcattg tggattgacc
    18121 atctccattt tgttatgggc ttaaacattg atacaacgtc catgttttga tgagattctt
    18181 tatggcgtag gttggaatct gtttggcctg taatagccaa gataggagat ctatccatat
    18241 tcgcacttgc aacacctgtt atcaaatttg tggcaccagg acccaatgta gataggcata
    18301 ccccaacttt ctgagttaat ctaccgtaaa catcagccat aaaagcagct ccttgttcat
    18361 gtcttactaa gataaactta atctgtgaat ctagtagcga aaacattaaa tcattagttt
    18421 cttcaccagg caaaccaaat atatattcca ctccttccga ttctaagcat cttatgaata
    18481 gatctgatgc cttaatcatt attggttata gtatgataat atagtatttc attattgtta
    18541 ttgtttggaa tgacagaaat gaggtaaaac aatacaaatg agataaactg aattcgtaac
    18601 acgatcgtgt taatttcaaa ttcttataaa cgcatgaata tactagtaac atttcggatt
    18661 ataatatatg ttacctcatc tatatttagt gaaatactca tagtataatc ttgattatct
    18721 caaaatcatc ctatatagta ctttaaatgg gtaacatgta ggttccaaat cagaaaagat
    18781 attatacttg attttatgtt aatgttttaa taacggtatc ccaaggagta tctttcgatc
    18841 ttttgcttcc aaaataagag aagagtgttt aaattcttga agtgattcca gccttataat
    18901 attgtcctct tttatttcaa tcatttgcgg aattttaaag ttaggggtcc tagtgtatag
    18961 tgcatagtgc tgaatgacgt ttaaaacatc taagtgaaac aatggatgac aaaccaaacc
    19021 tgataggcct gacacttgat agaataataa gcaattgaat tttcttaatt tttattacct
    19081 ttttatgaga ttgctatacc aggcagttat tacagaacta tcacaggaaa tgaacctatc
    19141 tacaattcgg tttactaagg acttgtgttt gggaactaaa aattacctct tcaaacacta
    19201 tatttatggc ttcactcagg gtatttaagg attgacccaa gttggtgtcc taatggtttc
    19261 ggtccatgta gggcctttat cctggaattg tggtgattca gatccatcgg ctacagtttg
    19321 tgtcctaatg gtttcggtcc atgtagggcc tttatcctgg aattgtggtg attcagatgc
    19381 gaaagtatta acattcttta cataaaatct atcttggtat ttatcatatc cgagatcatc
    19441 tatagatatg actttgaagc cattatcata taaatactgc atctccaagt aaaatagttc
    19501 gggtgatgta gcaatgtcat catcttttgt tatttgatga tatccaacaa ttggaatagc
    19561 atttatcttc cctggttcat tgaatttagt ttggctatta actactctga caaattctaa
    19621 atatagagag tatgaaacct ctattccgtg agggtcttga ttcttttcat tgttaatttt
    19681 ataatgagat ctatcatgtg accactcctt cattgcaaat ttatttgtag gtgttgtagt
    19741 tccgtcagaa aaatatggcc cacagtcagt cgttccatga taattttcct catcgtaacc
    19801 aaaattctcc catccatcac aattaagaaa cattaattca gaatgtcctg taaaggctaa
    19861 attaaaatag tttccaatag tatctacaat aaatggatcc tctccacctt tattgtaagg
    19921 agcttggaaa acagttggac taaaaccatt cttttgtaaa cattcttttc ctccactaat
    19981 aacagatgta atttcatttt ctgatttgag atcagcaagg cttgtatgtt ttaatccatg
    20041 agattgaatg tcatatcctt cagtgtctaa ttgtctcact tgattccaat tcataccttc
    20101 tccattttct atataatcac aggctatgaa tatactggct ttaaaaccaa atttatccaa
    20161 aatcggtttt gcttttgaga atattgattc atatccccta tcaaacatca ggattacaaa
    20221 tttatcctct ttttcttttt gttccttggc atatagagaa gataaatagt caaaaactaa
    20281 aacgtcatta gcagaggagt tgttgttgtt gttgttgtgt tgcaagttat cttcaaatgg
    20341 tgtctcaatt attattccat aggcagagac aatattggaa ggtattacat tgctattaac
    20401 tcctaaagat accactacaa aaagcataac ggcaaaacca ataaaaatat tcacaaaaaa
    20461 gaactctcaa ttatcttaat atggagtgcg tagaaattct atatattata atatcggatc
    20521 ttattgtgaa attcttttac tataaagtct atagtaacca cataattaaa aatttagaat
    20581 ttaaagaaag gtaaattagc agtaatctca gagccacgac tccaatcatt tatctctgca
    20641 aaatcatttc tatatatttg aatattgatt gaccatgata tgtcgttcaa tttacactta
    20701 aaaaatagtc tattggaata tgatattttg ctatagaaaa tatgctatct caaaattaat
    20761 tgcggttctt tccgaatttt gaaatcaaac ttattctcat cgaatgtcaa aacattgtca
    20821 agtacaagaa tttgtttgta ctactgataa ttcttatttt cacatactgc tcaaacactt
    20881 ctaccatctc ctctttctaa actctgtata tgaagaatgt cttctgtggt acatagtttc
    20941 ttctgaatct gtttgttatt cttttactct cttttttaga agtttataat ctagtctatg
    21001 ttctattttc agtttggtaa ttgaattaac taatataatt agctgtatca taggtcactg
    21061 ttaaaacaac caatacagtt caacatccta tatttaaaat tgaatttatt ataggagaac
    21121 cagttcctga tggttgataa taacataaag ctcatggaag aacaactaaa aatattttac
    21181 gtaatgaatt catctatttc tcatataacc tttaatagat tatttaaatc aaaaaaaaag
    21241 gaaaatgaaa aagataaaag aaacaaaaat atcttctttt gcatccacac tggtatattc
    21301 tagaataaac ctgattgcat tttttatttc agtttcagtc ttaatgttgt tgactttgat
    21361 atcaattgga gattatcatg acgattattt tccaaggcca gcctatgctg ctaaggcaga
    21421 atctaatcct gaaggaccaa ttattgacga tccaaacctt gtcgtccagc cagtctatca
    21481 aggacttaaa tcgcctactg ccatggcatt tatagcaaca aatgatatat tggttttgga
    21541 gaaagatgaa ggtacagtac agagaattgt aaacggcaaa attttacccg aaccattact
    21601 tcaggttgtt gtagattcta aagatgaaag agggatgcta ggaattgata tttctacaaa
    21661 ggatatggct accattggaa ctccacctag accttcccct atcaacgttt tcttattctt
    21721 tacggaagct aaactaggcg aatttcctat gggtaaccgt gtttacaagt atgaattaac
    21781 agacgataat actaaactag caaatcccaa attgctttta aacttaccag ctttgccgga
    21841 tgattctcac gttggcggag tggtcgctgt tggacctgat aataatgtat acctaacagt
    21901 tggtgaccaa aggccaactg catttaatag gattgtttac cctgattctc ataccaaagc
    21961 tcaaaattat ttaaatggaa tagaacctga tggaagatcg ggcatactaa gaattactca
    22021 agatggtggg acagtaggtg gggaaggaat tcttggggat agacacccac tcaataaata
    22081 ttatgcctat ggaataaaga atagctttgg acttgatttt gatcctgtta caggtaatct
    22141 atgggataca gagaatggac cgagctttgg tgatgaaatt aatcttgtca aaccagggtt
    22201 taatagcgga tgggctaata tacagggatt ttcgacatta gatgacgagt ggaataaagg
    22261 agaagaagta gtcaccccgc taaatcctaa ggattataac ttgatagact ttggtggaaa
    22321 aggaaaatat agttctcctg aatttacatg ggatgttgct ccgactgctc tacaattcct
    22381 taattctgat aaattaggta aacaatatga aaatgatatg tttgttggag atatcaaaaa
    22441 tggtaatctt taccattttg aactagacca acaaagaaca ggactactac ttcagggacc
    22501 acttgcagat aaggatgcga gtaaagacga aattaatcag gcagtctttg cacaaggatt
    22561 tggtggtatt acagatatag acgtaggacc tgatggttat ctgtatgtac ttacatttgg
    22621 tgaagaggat ggtacaatat ataaaatagt accagctgca aatggtgaaa aaaagatagt
    22681 ttcatagata tggaagacgg tggataataa tggcaatact aagatccgag atattaacag
    22741 tatctcttat tattttatct ctaataatta tgctgataac tccatcaatc agcatagaaa
    22801 aaagtggaat cgctatcaaa aatcattatg catattcttc tcctcctcct tctccttctt
    22861 attcttcaac agcggctgtt actttaggag attttaattt tgctgcagca ggagattggg
    22921 gttgtacatc taatacaata gacactgtgc aaaatattat agatcaagat ccagaatttg
    22981 ttttggcagt aggagatctt tcatatgaca gtagtgccaa atgctggttt gagatcatat
    23041 cccccataga aaacaaaact atgatcgcaa ttggaaatca cgatactgac ccatctatgc
    23101 ttaaagagta tatggatttt tttggattaa aaggacaata ctattcattt aactatcaaa
    23161 atgttcactt cattgtaatg tcaacggaac ttccatatga agacggttct gagcagtata
    23221 actttgtaaa taatgatctt tcaaaagtat ctttaaatcc tgatattgat tggattgttg
    23281 tggattacca tagtttagcg tatacatctc cagcaaatat tggtaaaggc aatagtgcag
    23341 aaaaggaatt gagagatatt tatcatccac tattcattaa atacaacgtt gatctagtat
    23401 tgcaggcaca taatcacaac taccaaagat catatccgat aatctacaac aatactaatt
    23461 cagagaatcc tataataaca gacaccaaca acaataacaa ctactatgat ccaaaaggta
    23521 tgatatttgg tacagtcggt actggtggtg aaagccttta tcctttaaca ggacaagcac
    23581 cttatgttgc tacccagtat gtaggctttg gctttttgaa tgtagatgta ataaatgatg
    23641 gaacaactct tagtggtaaa ttctttgcta acgatggaac aataaaagac caatttacca
    23701 ttataaaatc agttaatatt agtgaaaaca ctaaataaat ttaaattaag gctaccaaag
    23761 aatagtaaca tagaaagttt tattttttat tttttttaga tctgagcctt gaattgaata
    23821 taacaatata tctcttataa gatgccatag taatataatt tcagatgaaa attagtttgg
    23881 atgactttta atcgtatgaa tatttttatt ttcgaactgt caacgaacaa attaggttta
    23941 acaagatctg gtaccagttg gttattactt gaaacgttga atatataatt cttgaagatg
    24001 gattctctgg atttgtatct gtcctgtcct atcacgagtt tcagttgcat tttctattgt
    24061 tgttgttttt gtgtagtaat ttggtctatt atttttttat acgagtcaat aaaacttttt
    24121 gcatacgtat tggcatattt ctctgatccc ctgtgtatac caccttttgt tcgaatatga
    24181 tgataaaatt catgtaagat tataaaggga ttataaaata catcggagtt tatacaataa
    24241 atcttgcttt ctctttgaac ataaactgca taaacagtct ttcttttccc ttttatggtt
    24301 ccaacagtta tttcaggtgg gataacttta tagaatttac tcagattttt taacgcttct
    24361 tctgtttttt tattaaggat catattgact actttagcct tagttatatc atcaggatgc
    24421 acaatagtgg acatgttata tatttcgcct tgaaatttta taagaataaa tatataaaat
    24481 aattactatt ctattattta aaataaattt attttagagg aatgtattgc agaaattgaa
    24541 taaatatata acgttgtatg tttaattata ggaatctgcg tgatactacc aaatcctata
    24601 aaattgactg ttcttagtac cattttcgat aatagttgtt ggcgctaaat aaatacataa
    24661 aaaccagtaa tgaagaccat aaaactaact actaataaac ccctttgctg tataattgaa
    24721 tatttttgta caagtttgaa gaatagcaga agaaaatatt tgattaggaa ttggattcaa
    24781 gatagttttc aacagttaga atatgtgtat gtatgtatgt aaatatagct tcacactata
    24841 caatcaatct attagaaaat tttttaataa aattaacatt attttggaga gatgaagata
    24901 gaaattatat aattgcataa aaatatcaat tactttatga atttaaagca tataccgcta
    24961 tataaaatgc agaatcatta caattatgag agataattat ggtaaaatta taaaatcaga
    25021 aaatctcatt aataagaaag caaaaggtat tgataatat
//
LOCUS       DASVEV010000155         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1610817, whole genome shotgun sequence.
ACCESSION   DASVEV010000155 DASVEV010000000
VERSION     DASVEV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610817"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF222_07180"
     CDS             <1..222
                     /locus_tag="VF222_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142244.1"
                     /translation="KSNDYKRDAQNDEIKSIVLGLFALIGFLILIYSIFMGVVGEPLA
                     SVADTVFVAAIIGSFTLVGIIVGSVFLKK"
     gene            complement(762..1076)
                     /locus_tag="VF222_07185"
     CDS             complement(762..1076)
                     /locus_tag="VF222_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142245.1"
                     /translation="MDSQFDFIQNIQETLKISKSSITAYNNETVNRLYLSRVKILKSF
                     LGQSNDNNNLDKLKKLILFEIQQLKKKKKKTIYRIETIRIIEEIDNLEFCLNAIVQDI
                     NK"
     gene            complement(1195..1845)
                     /locus_tag="VF222_07190"
     CDS             complement(1195..1845)
                     /locus_tag="VF222_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142246.1"
                     /translation="MSYGIFREIERLIEYIPIEIDTYGIGKLSSNTFIDSRYNFKDTD
                     RIATRLGILEVGRNTVSLRDIIKLLNKFLKDAEIEELVESPRRYRELQNSSSGLPLKN
                     LLFDFCLSALVGDTFDAKLFLTHSLYLTGLRRQLVILLSSVINDLGLVNNDNDIREML
                     TLLALLSEPNNIDVYFDINNEGFNKRYNTYLKEIGKLNYNINSNIPLAKISQEKKI"
     gene            2145..2498
                     /locus_tag="VF222_07195"
     CDS             2145..2498
                     /locus_tag="VF222_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX7142247.1"
                     /translation="MNTSILKNQKYQTCVDACNNCAECCEACCTECMSNTEMAGMMGR
                     CMMMCRDCADMCRMASMMMARGSEYVKQMCNMCADMCEACAMECEKMGGKMEICKECA
                     EMCRMCAQECRNMTR"
BASE COUNT          903 a          330 c          370 g          998 t
ORIGIN      
        1 aagtctaatg attataaaag agatgctcaa aatgatgaaa ttaaatccat agttttagga
       61 ctattcgcat tgattggatt tcttatcctt atatattcaa tatttatggg agtcgtaggt
      121 gaaccattag catccgtagc agatactgta tttgttgctg caattattgg gtcttttact
      181 ttggtaggta taattgttgg cagtgtattt ttaaagaaat aatatctttt attttattta
      241 ttaatcaata tactaatcaa ccttattatt aattctttga tacagaaatc aagttaaatt
      301 aaaaaaagtt tcctaggttt agtgaaatat tataatatgc ctataatttg tttacgttgt
      361 tttaaagtag attattttat taaaatatta aaccattgaa gttagtagaa ttccagatga
      421 gggttttgag agaattgaga gaactcacct agcctctaag tagggtggcg cattcaattt
      481 gcttattttt tcttatttat gaaaatattc ttgcatacta taatatagga acataagata
      541 ttttattgat agtaaagatg caaacaaaca ctagaatgtg aattttatac atattattca
      601 taaatcacag gatattataa aataatcgtt atggcaaggg tagtaaatct tatacaaaca
      661 ttcacatctg attgcctgtc aagttgaaat cagtatatca gacacatatg acttgaaaaa
      721 taggaataaa actatttatc agagttaatt tatttattta tttatttatt tatatcttga
      781 actattgcat tcaaacaaaa ttctaaatta tcaatttcct ctataattct aatagtttca
      841 atgcgataaa tcgtcttttt ctttttcttc tttaattgct gaatttcaaa taaaattagc
      901 ttcttaagtt tatctaaatt gttattatca ttactctgac ctaaaaacga ttttaatatt
      961 ttaacacgtg aaagatatag tcgattgact gtttcattgt tataggctgt tatggatgat
     1021 ttgcttatct ttagtgtttc ttgaatgttt tgtataaaat caaattgcga atccaaaatt
     1081 attcaattct cctgttttga ttctttgaac acggataggc tattacgttc ctttaataat
     1141 ttttaatata gaattctatt cttgatatat tatagtaaac cattgaatac tatattatat
     1201 ctttttttct tgactaattt tagctagtgg tatattagaa ttaatattat agttgagttt
     1261 acctatttct tttaaatatg tattatatct cttattaaat ccctcattat taatatcaaa
     1321 ataaacatca atattatttg gttcagaaag taatgcaaga agtgtcagca tctcccgaat
     1381 gtcattatca ttgttcacaa gtcctaaatc gtttattaca gaagaaagca atataactaa
     1441 ttgtctacgg agtccagtaa gatataatga atgtgtaaga aataatttag cgtcaaatgt
     1501 atcacctact aatgctgata aacaaaaatc aaataacaaa tttttcaatg gaagaccaga
     1561 gctactgttt tgtaattctc tataacgtct cggactctct acgagttctt caatctctgc
     1621 atctttgaga aatttattta aaagttttat gatatctctt aagctaacag tatttctacc
     1681 tacttctaga atccctaatc ttgtggcaat tctatctgta tctttaaagt tgtatctaga
     1741 atctataaat gtattagaac ttagttttcc tattccataa gtgtctattt caattggaat
     1801 atattctatc aatctttcta tttctctaaa tattccatat gacatttttt gttatcatta
     1861 ataatgttga gtgtattatt tattatcctt aggaaatatg taaattatta aaagtatatg
     1921 caaagcagaa cattggagat ataatttttc atacatttag ttctataatt tttcaccatc
     1981 acgtaagtta ttttaatttt atatgcaaat cttgcataga gaattatgca atatttacat
     2041 ataaatttaa taaatgagta atttatacta aatatatgtc atttttacat atatattaca
     2101 atttttaact caatccttat atactatgca gatagactaa aattatgaat acttcaattt
     2161 taaaaaatca aaaataccaa acttgtgtag atgcatgtaa caattgtgca gaatgttgtg
     2221 aagcttgttg taccgaatgt atgtctaaca ccgaaatggc aggaatgatg ggacgctgta
     2281 tgatgatgtg tcgggattgt gcagacatgt gcagaatggc ttctatgatg atggccagag
     2341 gtagcgagta cgtaaaacaa atgtgcaata tgtgtgcaga tatgtgtgaa gcatgtgcaa
     2401 tggaatgtga aaagatgggt ggaaaaatgg aaatttgtaa agaatgcgca gaaatgtgca
     2461 gaatgtgtgc acaagaatgt cgcaatatga cacgataaat aatataatat tgattataaa
     2521 acaaacttca gatgtctaag ataaaatcaa cactattttt ttctaattaa tagttttttt
     2581 atttaatcat agctatatgc t
//
LOCUS       DASVEV010000156         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_445566, whole genome shotgun sequence.
ACCESSION   DASVEV010000156 DASVEV010000000
VERSION     DASVEV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445566"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF222_07200"
     CDS             complement(<1..530)
                     /locus_tag="VF222_07200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7142248.1"
                     /translation="MDTTQKKGRRQNQAKINTQNKKKNINSNNTRNKSKIAKVNNYVS
                     NKAKIGKNVKIWHFAYVGDNTIIGDNVMIGSLAHIDYAVKIGNNTRIEGSVYIPPLTV
                     IGNNVFIAPGATFTNDPYPMSPKMIGVTVEDGAIIAARAVIKPGVTIGKNAVVGMGAV
                     VTKDVPPETVVIGNPA"
     gene            complement(556..954)
                     /locus_tag="VF222_07205"
     CDS             complement(556..954)
                     /locus_tag="VF222_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7142249.1"
                     /translation="MKKHMLDILACPIDKNYPLELIELNVKDLEKEFLKENNYPINNE
                     EEQNNFIKKNNRYKVNQENEIKDNEKVIVIIDGILYCKKCSRFYPIIDEIPIMLPDEL
                     REKENDLQFLKKWKDCIPEDILKNSNPWHL"
     gene            complement(961..2037)
                     /locus_tag="VF222_07210"
     CDS             complement(961..2037)
                     /locus_tag="VF222_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7142250.1"
                     /translation="MVEDIIHSPQRDFNGGNLIELGKEKDMMDDKSKNLHKNGSQNNN
                     AVKIGIIGVGGWGKNHSRVLHDFGVLTSICDMDKTKAQNLATRYNVNYYGSMDDMINS
                     ESLDACLVCTPTKTHYSVTNKLMEKGINVFVEKPLSYSSKECEEMIQIAKKNKISLTC
                     GYIERFNPAVQEVKKLLSDKIYGELLMIEFHRENRMPLHIKDVGIIYDTSVHDIDTAI
                     YLFGTKPQVVFARAGKIFHSFEDFSNIMLGFGKDRVAVISSNWITPKKVRNFSAVCTD
                     AIINGDFIKQEITIEDSLSTTTPRRQNLVEPLSLELRNFIEVIEGKRERPEVSPEEAT
                     NVTKIAEAALLSSNTGAPIYLNLG"
     gene            complement(2031..2954)
                     /locus_tag="VF222_07215"
     CDS             complement(2031..2954)
                     /locus_tag="VF222_07215"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7142251.1"
                     /translation="MKNKGAKYIWFDGEFIEWEKALIPINTHALHYGTSIFEGIRAYK
                     SNSNLHIFKLKEHMERLFRSASIYSIKINHSVDELVDGTVQLLRKVNMRESCYIRPLS
                     FVGVHGIDLNVTYESPTHTLIIVFPFAKYFNTEGIRVGVSSWRRIHDSSIPPVAKAGG
                     NYLNSILATQECKRNGYDEAIMLDKNGNVSEAPGENIFLIKNKTIFTPDMSSSILEGI
                     TRDTVIALAQDLGYTLIQRPISRSELYIAEEIFLTGTAAEIIGVVSVDGRNIGNGNEG
                     QITKKIRNTYNEIVTCNNNSEKYKGWTTEVW"
     gene            complement(3009..3635)
                     /locus_tag="VF222_07220"
     CDS             complement(3009..3635)
                     /locus_tag="VF222_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142252.1"
                     /translation="MEKLAALYLAHLNPFTKAHQEIIFGLQKNYVVYVFPVRFLKNGK
                     EMNTRSFPFPYDIRKSMIESVFPNEEKVIVSPNYTFFAPFIKYFPPLFSPSSWKIREQ
                     VLKPVREKKFISYTGDRVERYALRIYRFNPIKAKRLSISATQVKEDIYDEALSKRDNL
                     KNSQWKDKIPNPVIKIIEDNWSIVEKFANKKDETLKVVGVKLPRKGFF"
     gene            complement(3768..4037)
                     /locus_tag="VF222_07225"
     CDS             complement(3768..4037)
                     /locus_tag="VF222_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7142253.1"
                     /translation="MRDAIYIGKKPLMAYVTSTLIQLANQPKVTIKARGLSIGRAVDV
                     SQIILKRMENAGYTIGDVKIGSETVQSEDGRTRNVSTIEIVVTRS"
BASE COUNT         1202 a          720 c          578 g         1542 t
ORIGIN      
        1 ctagcaggat tgccaataac gactgtttca ggtggcacat cttttgttac aacagcaccc
       61 ataccaacta cagcattttt tccaatggta accccaggtt ttattactgc tctagcagcg
      121 attattgctc catcctctac agtcactcct atcatttttg gactcatggg atatggatca
      181 ttagtgaacg ttgcgcctgg tgcaatgaat acattgtttc ctattactgt taacggtgga
      241 atatatacag agccctcaat tctggtgtta tttccaattt taacggcata atcaatatga
      301 gcaagagaac caatcataac attgtctcct attatggtat tatcacctac atatgcaaaa
      361 tgccatatct ttacattctt tcctattttt gctttatttg atacgtaatt gtttactttt
      421 gcaattttac ttttgtttcg agtattatta ctattaatat tctttttttt attttgcgta
      481 ttaattttag cctgattttg acgacggcct tttttttgtg tagtatccat ttattttaga
      541 gcagctatga ataacctata aatgccacgg attagaattt tttaaaatat cttctgggat
      601 gcaatctttc cattttttta aaaattgaag gtcgttttct ttttctcgta gttcatcagg
      661 taacatgatt ggaatctcgt caataatagg ataaaaacga gaacattttt tacaatataa
      721 tattccatca attatgacaa taactttctc attgtcctta atttcattct cttggtttac
      781 cttatatcta ttattttttt ttataaaatt attctgctcc tcctcattat ttatcggata
      841 gttattctct tttaggaatt ctttttctaa atctttcaca tttaactcga ttaattccaa
      901 aggatagttt ttatctatcg gacaagccag tatgtccagc atatgttttt tcatttcaca
      961 tcatcctaaa ttaagataaa ttggagctcc tgtattacta gataaaagtg ctgcctctgc
     1021 aatcttggtt acgttagtag cttcttctgg agatacctct ggacgctctc tttttccttc
     1081 aataacctcg ataaaatttc ttaattcaag tgaaagaggt tcaactaaat tctgccttct
     1141 gggagtagtc gtggaaagag aatcttctat agttatttcc tgtttgataa aatcaccatt
     1201 gattattgca tcagtgcaaa cagcgctaaa gtttctaacc ttttttggtg taatccaatt
     1261 tgaagaaatt acggctactc tatcttttcc aaagcctagc ataatattag aaaaatcttc
     1321 aaaagagtga aaaatctttc ctgctcttgc aaatacaact tgtggtttag tcccaaaaag
     1381 gtatattgcg gtatctatat catgaactga ggtatcatag ataattccaa catccttgat
     1441 atgtaaaggc attctatttt cccgatgaaa ttcaatcatc aataactctc cataaatttt
     1501 atcagatagt aattttttta cctcttgaac agctgggtta aatctttcaa tatatccaca
     1561 tgtcagtgat attttattct tttttgctat ttgtatcatt tcttcgcact cctttgatga
     1621 gtatgataat ggtttttcaa caaatacatt gattcctttt tccatcaatt tgttggtaac
     1681 tgaataatgt gtctttgttg gagtacaaac aaggcaagca tctaaacttt ctgaatttat
     1741 catatcatcc attgaaccat aataatttac attataccgt gttgccaggt tttgtgcttt
     1801 agttttatcc atatcacata ttgacgttag gacaccaaaa tcatgaagaa ctcttgaatg
     1861 attttttccc caaccaccaa caccaatgat accaattttt acagcattat tattctgtga
     1921 tccattttta tgtaaatttt ttgatttgtc atccatcata tctttttctt ttcctaattc
     1981 tattagattg ccgccgttga aatctctctg gggagagtgg ataatatctt ctaccatacc
     2041 tcagtagtcc atcctttgta tttctcacta ttgttattac aggtaactat ttcattgtag
     2101 gtatttctta tttttttagt tatttgacct tcatttccat ttccaatatt tcttccatca
     2161 acgctaacaa caccaattat ctcagcagct gttccagtca agaaaatctc ttctgcaata
     2221 taaagttctg agcgtgatat tggtctttga ataagagtgt aaccaagatc ttgtgcgagt
     2281 gcaattactg tgtctctcgt aattccttct agaattgatg aagacatgtc aggagtaaat
     2341 atagttttgt tcttgatgag aaaaatattt tcgccaggag cttcacttac atttccattt
     2401 ttgtcaagca tgatagcttc atcatatcca tttcttttac attcttgtgt tgccagaatg
     2461 gaattaagat aattcccccc agccttagca acgggaggaa tagatgagtc atgaattctt
     2521 ctccatgagg aaactcctac cctaatacct tctgtattga agtattttgc aaaaggaaat
     2581 actataatta acgtgtgagt gggagattca taagtaacat ttagatctat accatgaact
     2641 ccaacaaatg aaagtggtct aatgtaacat gattccctca tgttaacttt tcgtaataac
     2701 tgtactgtac catctaccaa ttcatctaca gaatgattaa ttttaataga gtatattgag
     2761 gcggagcgaa aaagtctttc catatgctct tttagtttaa aaatatgtaa attgctattt
     2821 gatttataag ctctaattcc ttcaaagatt gaagttccgt aatgtaatgc atgtgtattt
     2881 atcggaatta atgctttttc ccattcaata aattcaccgt caaaccaaat gtattttgct
     2941 cccttgttct tcattttaag aaacgtattt ttaagaatta atataaaagt ttactataat
     3001 aacgagattt aaaaaaatcc ttttctaggt aattttactc caactacttt taaagtttca
     3061 tcttttttat tcgcaaattt ttcaactata ctccaattat cttctatgat tttgattaca
     3121 gggtttggaa ttttatcttt ccattgggaa ttctttagat tatctctttt actcagagcc
     3181 tcatcataaa tatcttcttt aacctgagta gcagaaatag aaagtctttt ggctttgatt
     3241 ggattaaatc tataaatcct cagagcatac ctttctactc ggtcaccagt atatgatata
     3301 aatttttttt ctctaactgg ctttaaaacc tgttctctaa ttttccagga agagggggaa
     3361 aaaagcggtg gaaaatactt gataaaagga gcgaagaatg tgtaattagg tgacactatg
     3421 actttttctt cgtttggaaa aacagattct atcattgatt ttctaatatc atagggaaag
     3481 ggaaagctgc gagtattcat ctcctttccg tttttcaaaa atcgtactgg gaaaacatat
     3541 actacataat ttttctgtaa accgaatatt atctcttgat gagcttttgt aaatggattg
     3601 agatgagcta aataaagtgc agcaagtttt tccataagcc aactaatact aaataatctt
     3661 gcttttaatt tgattaaaat taatgatatt aataattatt ataataattg tattactgta
     3721 aaaataacaa aaattaatgt tagttttcaa aaaactaact aactaaccta gcttcgtgtg
     3781 actactattt cgattgtaga gacgtttctg gttcttccat cttctgattg tacagtctct
     3841 gaaccaatct ttacatcacc aatagtgtag cctgcattct ccattcgttt caatataatt
     3901 tgtgaaacat caacggctct gcctatactc aatcctcttg ctttaatggt tactttaggc
     3961 tgatttgcta actgaattaa cgttgatgta acatatgcca tcaatggttt ttttccaata
     4021 tatattgcat ctcgtatttg ag
//
LOCUS       DASVEV010000157         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_446714, whole genome shotgun sequence.
ACCESSION   DASVEV010000157 DASVEV010000000
VERSION     DASVEV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446714"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..1362)
                     /locus_tag="VF222_07230"
     CDS             complement(394..1362)
                     /locus_tag="VF222_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142254.1"
                     /translation="MAIFQRYSKNPILTPNKDNWWESKAVFNCSVSFDGKNVHMLYRA
                     IGEYDHYISRIGYASSSDGFQFDRRHNIAIHPTEDYEKNGMEDPRITQIDDKIFVTYV
                     VLSEYVKNHPKIFSALAVTKNYDEFDKLGIITKDFDNNKDVIFFPDKFKVNSDFSNNS
                     SFLILHRPTSLSNPDYQLSRPSIWLSISDSESTLTNSMLLLKPEQDWENLKIGAGPSP
                     IKTKKGWLLIYHGVSTDKIYRAGAALLDLDDPRKVIARTKQPILEPVEDYEKIGDVNN
                     VVFPTGTVVIDKKLLLYYGGADRVCCVASASIDELIEHILKDSITS"
     gene            1521..2744
                     /locus_tag="VF222_07235"
     CDS             1521..2744
                     /locus_tag="VF222_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX7142255.1"
                     /translation="MNLKDDSINLHRMLKGKIEIQNRVPIENIFAKENSLINLIYTPG
                     VAYVASEIGENKDLAYDFTSKWNNVAIICDGTRVLGLGNIGPDAALPVMEGKSVLFKL
                     LGGINAIPLCIATQNKEEIIKFVKYIQPLFGAINIEDIESPKVLEIVERLKQEISIPV
                     FHDDQHGTAIITLAALINSLKLVEKQLEKIKVLILGAGSAGYGINKILNSAGCKNTIV
                     VDSNGAIYEGRKDLINNQFKSEIASNSNPAKIEGSLDSVIDGSDVFIGVSGKGNILNY
                     KVISTMNVDPIVFALSNPDPEILPPFALDAGAKIVATGRSDFPNQVNNAVIFPSVIRS
                     LLDLRVKLLDDNLMVNVAYAIANLVESQHLKPDYIIPQINDQRILPVVSQTLNRVIQK
                     TVTDNYEKETNDDEH"
     gene            2941..3657
                     /locus_tag="VF222_07240"
     CDS             2941..3657
                     /locus_tag="VF222_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX7142256.1"
                     /translation="MRNNSKKLLASVITSILFTIFAVSFFSIDDLKADAQSANATNYS
                     FEPFQSLQGSTYQDIPDSDSLNLTEFSLSSWFRTSPNYTEPGHIVNKGGMNTDEQGMN
                     LNYGLWINEENAVAGGFEAESGDDFILASSDKFNDGNWHYVVLTFNGTSLFLYIDGKL
                     QGNLTSIEEAPDQTGDQPLRIGANSLNEDKFFTGDVDEIRVWNRGLNMSEVENSYGNN
                     KFNTTGQKLFLSYGDYVNSK"
     gene            4025..4843
                     /locus_tag="VF222_07245"
     CDS             4025..4843
                     /locus_tag="VF222_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX7142257.1"
                     /translation="MYRNLYFAIKYSNLILSELLLVNITLIFDTVLLPLLGLLERFLS
                     IFRAKAHSVADRFEDPNEMLNYSFEKQHELINKLRRDITTVVTSKKRLEIQKAKLSTN
                     ISTLEQQARRSLESNREDLARLALERKNLLLTQIKELERQIGDIEKEQIKLEDAERRL
                     SSKIEEFKTRKEMIKAEYSAAEAQVKIKENMTGISEEMNDLGMVLNRVEEKTENMKAK
                     AQALDEMIDSGTLTDITTSYPAGNADIEKELDKVSIERSVEEELKKLKTEVGKT"
BASE COUNT         2038 a          745 c          921 g         1939 t
ORIGIN      
        1 attctttaat ggaaattggg tatgtgatga ttgttttcta aaacaaagag gaatagaatc
       61 agaatctata gactattcaa cagaagaaaa ggataaatct aaagatattg catcatctag
      121 agatgtatct cctaatactc ccactgcttg atttataatt ccttcaccta ttgtattgtt
      181 accaactaga tagacctaaa ttttatccat tatttgtcta taaataaaat ataagggtgc
      241 cactgcgata tatactagat tggaaatcct tttatttctg ttaattttta taaaaaatag
      301 taatgtccag agactgaaat acaacaagta tacttatata tgtttaaaat aaggtaaata
      361 aaattttaga tacaagctta taagaggtag tatctaggag gttattgaat cttttaaaat
      421 gtgttcaata agttcatcaa tactggcgct tgctacacaa catactcgat ctgcaccccc
      481 ataatataat aagagttttt tatcaattac cactgtacca gtcggaaaaa caacgttatt
      541 aacatctcct attttttcat agtcttctac tggttctaag ataggttgtt tcgttcgagc
      601 aataactttc cgtggatcat caagatccaa aagtgctgct cctgcgcgat aaattttgtc
      661 ggtacttaca ccatgataaa ttaatagcca tccttttttt gttttaatag gtgacgggcc
      721 tgcaccaatt ttgaggttct cccagtcttg ttctggtttt aaaagtaaca tagaattagt
      781 taatgttgat tcagaatcac ttatactcaa ccatatacta ggtcgactta gctgataatc
      841 aggattactc aaactggtag gtctatgtaa tatgagaaag gaactattat tagaaaaatc
      901 agagtttacc ttgaacttat ctggaaaaaa aattacgtct ttgttattat caaagtcttt
      961 ggtaataata cctaatttat caaattcatc ataatttttc gttactgcta gtgcagagaa
     1021 tattttggga tgatttttta cgtactctga aagaactaca taagtaacaa agattttatc
     1081 atcaatttgt gttattcgcg ggtcttccat gccgtttttt tcatagtctt ctgtcggatg
     1141 aatcgcaatg ttatgacgtc tatcaaattg gaaaccgtca ctgctacttg cataacctat
     1201 tcgagaaata taatgatcat attctccaat tgctcgatag agcatatgaa cattcttgcc
     1261 atcaaaggat accgaacaat taaacactgc ctttgattcc caccagttat ctttattggg
     1321 agttagtatc ggattttttg aatatctttg aaatatcgcc attaacgagc ctaattattg
     1381 ttatagtatc actataatta gtattcgggt gaatatttcg ggattgaaaa tagtaaactt
     1441 tcaactagtt aaattatttg tttatgcaat ttttttgtat tagaagaact attataatat
     1501 tctatattta atgttcttac ttgaatttaa aagatgattc tattaattta catcgaatgt
     1561 tgaaaggtaa aatagaaatt caaaatcgtg tgcctataga gaatattttt gcaaaagaaa
     1621 acagtctaat taacttaatt tatactccag gtgttgcata tgtggcctca gagataggag
     1681 aaaataaaga tttagcctat gactttactt caaaatggaa taatgtggca attatatgtg
     1741 atggaacaag agtgcttggt cttggaaata taggtcctga tgccgcgctt ccagtaatgg
     1801 aagggaaatc agttcttttt aaattattag gaggtataaa tgctatacct ctatgtattg
     1861 ccactcaaaa taaagaagaa ataataaaat ttgtaaaata tattcaacct ttatttggtg
     1921 ctatcaatat agaggatata gaatcgccta aagttctaga aattgtagaa agactaaaac
     1981 aagaaatttc tataccagta tttcatgatg atcaacacgg aactgctata attacattag
     2041 ccgcattgat aaattctctt aaacttgtgg aaaagcagct ggaaaaaatt aaagttttaa
     2101 ttttaggggc aggttctgcg gggtatggaa taaataaaat tcttaattct gctggttgta
     2161 agaatacaat agtagttgat agcaatggtg ctatttatga aggacgcaaa gatttgatca
     2221 acaatcaatt taaatctgaa atagcatcta actctaatcc tgcaaaaata gagggttcat
     2281 tagattctgt aattgacgga tcagatgtat ttataggagt ttcgggaaaa ggtaacattt
     2341 taaattataa agttatatcg actatgaatg ttgatccaat agtttttgca ttaagtaatc
     2401 cagatcctga aatattgcct ccatttgctt tggatgcagg tgcaaaaatt gtggccacgg
     2461 gtaggtctga ttttcccaac caagttaaca atgctgttat ctttccatca gtaattagat
     2521 ctttactaga tctacgtgtt aaattgttgg atgataattt gatggttaat gttgcatatg
     2581 ctatagcaaa tttagtagaa agccaacatc tcaaacctga ttatataata ccgcagataa
     2641 atgaccaaag aattctacct gttgtttctc agacgttaaa ccgtgtaatt caaaagactg
     2701 tgacagataa ttatgagaaa gaaactaatg atgatgagca ctaggaaggt aaatttttta
     2761 aatgaactaa tacaataaaa attttatttg tttatttctt gaactagttg tatgtttata
     2821 aaaaaattgc aatgtgtata gggaaagaga tcgtataatt attttatctt atataagtat
     2881 attaaaaaaa actgaataat tcatggtcat ctatttatac tttctatgct ttctcatgac
     2941 atgcgaaaca atagtaaaaa attactggca tcggttatca catcgatttt atttaccatc
     3001 tttgcggtct cttttttctc tattgatgac ctaaaagctg atgcacaatc agcaaatgct
     3061 acaaattata gttttgaacc tttccagtct ttgcagggtt cgacatatca agatattcca
     3121 gacagtgatt cactgaacct tactgagttt agtttatcat cttggtttag gaccagtcca
     3181 aattatactg aacctggtca tatagtgaac aaaggtggaa tgaataccga tgaacaagga
     3241 atgaatttaa attatggact ttggattaac gaagaaaatg cagtagcagg tggatttgag
     3301 gcagaatcgg gagatgattt cattttagca tcatcagaca aatttaatga tggaaattgg
     3361 cattatgtag ttttaacatt taatggtact tcattatttc tttatataga cggaaaatta
     3421 caggggaatc taacatctat tgaagaagca ccggatcaga caggtgatca gccattacga
     3481 attggagcca attcgttaaa tgaggacaaa ttctttacag gagatgtaga cgaaataaga
     3541 gtatggaata gaggattaaa tatgtctgaa gtagagaact catacggaaa caataaattt
     3601 aatacaacag gacagaagct gttcttatca tatggtgatt atgtcaatag taaataaaag
     3661 cctacttcta taaaataaac atatttatta tttttatttt ttaaaagtaa tgtttgtgat
     3721 gttatgttct gatctattgt tgatatatta attatgatat taaaattcta atggggaata
     3781 tcagaatgta aattttatac tatctttggg tgaggaagat atatatacta atgaattttc
     3841 atttattttg aaatattgtt gcattttgtt cctaaattta atcaatcaat cacattttgg
     3901 ttctctatat cggtttaatt gttttatttg tagtttaatc ataaactatc aggacttatt
     3961 attattgtct catctgaata agaatgcata atattagaat aatatggaat aaaatgaatt
     4021 tatattgtat agaaatctat attttgctat aaaatattct aacctgatac tatcagaatt
     4081 gctcttagtg aatataacat taatatttga tactgttcta ttacctttat tgggattatt
     4141 agaacgcttt ttatcgatat tcagagcaaa agctcattct gttgctgatc gatttgaaga
     4201 tccgaatgaa atgctaaatt attcttttga aaaacaacac gaattgatca ataaattaag
     4261 acgggatata acaactgttg taacttcaaa aaaacgactt gaaattcaaa aagcaaaact
     4321 tagtactaat atttctacct tagaacaaca agctcgtcgt tcactggaat caaataggga
     4381 agatttagcc agattagcac tagaaagaaa gaatttgctt cttacacaaa ttaaagaatt
     4441 agaaagacag attggtgata tagaaaaaga acaaattaaa ttagaggatg ctgaaagacg
     4501 gctttcttct aaaattgaag aattcaagac acgtaaagaa atgataaaag ctgaatattc
     4561 agctgcggaa gctcaagtca agatcaagga aaatatgacc gggatatcag aagaaatgaa
     4621 tgaccttggt atggtgttaa acagggtaga ggaaaagaca gaaaacatga aggctaaggc
     4681 acaggcatta gatgaaatga tagattctgg aacactaact gacattacaa catcttatcc
     4741 tgctggtaat gcagatattg aaaaagaact tgataaagtt tctatagaga ggtctgtcga
     4801 agaagaatta aaaaagttaa aaactgaagt cggtaaaact tagttattat tgtattgata
     4861 aaatagtaaa attgtataga tagaacggat cttattgtta gaatattggt aaatgacaat
     4921 tcaaagttaa ttcagaacta ttagacataa ttaaggtaat tgataactca ctagtagaat
     4981 ttgattgaaa atcaatttcc atgatcaact accataaact tactactcaa acagaactat
     5041 atgaaaaagt aacagaaata attagtttca tcgagctaaa tgaacaatca ttagatgctg
     5101 aaaaaaattt gtgagatctg atactataat cctagattcc gattctttta ttgaagaagc
     5161 aacaataagt tttagagaag aaggaataat tttacataag taggtttgta tttgaaattt
     5221 aaaaactttt aataaaaact tatcttataa tatcttttat tacttttaat tataattcta
     5281 ggattgagaa atctattaaa agtaacatat tattaagctt aggaaattaa aagcaaagta
     5341 taataataga aaaatgtaaa ttgttatcct attatggata aatcttttat taagaatgtt
     5401 cttattattg tgttattaat tattgtgggc ttaatactac taaaattttt gactcaatta
     5461 gttataatag ttgtgataat tgggggagct tatttattgt atagactata tttaaaaaat
     5521 aaacgtaaat tttaagaaat tcctttacat aattaaaaat ttattttttt aattatcttc
     5581 cttgcataaa atttgatttt tattaggaat ttagctttat ttatcttaat ctttctcaga
     5641 ttt
//
LOCUS       DASVEV010000158        11385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_446817, whole genome shotgun sequence.
ACCESSION   DASVEV010000158 DASVEV010000000
VERSION     DASVEV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446817"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF222_07250"
     CDS             complement(<1..314)
                     /locus_tag="VF222_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7142258.1"
                     /translation="MDKYLIRYYDIFNCSSDRPTILLLHGLGGSADRWTKIIPSLSSK
                     YRLIIPDIIGFGYSEKPHVEYTVDFFINFLKKFLDTLHVSDPIIIGSSFGGLLALEYA
                     IK"
     gene            complement(1296..2546)
                     /locus_tag="VF222_07255"
     CDS             complement(1296..2546)
                     /locus_tag="VF222_07255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7142259.1"
                     /translation="MKNNIKIIHEDLNAGGGSERLTITMMEALNEMGFNIDLETISTP
                     DWNQIKKWYGKFSLRIEKIKKIDLSNVFGSSECANEKKKQIALEEEKYTLIINTHGDI
                     LPYYNYTSHRKLKTKIITYCHYPLTPRLINHGSYKKFLMKFLQNSIVDYERNSHVKEK
                     ELLYRILSTYDLMMKNTLIVTNSQFSKQAIENYYGKVVLSILSPPVDVETYRKVALSN
                     LIRDNTIIVISRYSPDKKIETAIRIARILHKFFKNFKMILIGNIVKDNYDYYRYLQSL
                     ISMYRLNEHVKIQLNVPLDSVLKMMATSKILLHPTLEEPFGMSVAEGMSAGLIPVVPF
                     KGGNTEFVPSRFHFKTEEEAVKIIIDSMKISFEDRLKISKYPDIFSVNNFKSTLKTII
                     QNILEKELVISSPQLKNNNIINRN"
     gene            2988..3377
                     /locus_tag="VF222_07260"
     CDS             2988..3377
                     /locus_tag="VF222_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142260.1"
                     /translation="MKNSSRINYIFLLLSFGIVSTILSSPISAQTQNNSQNQSAGTAS
                     SGPYATGKPGIGSADELESITNDTQTSTSNDTTPMMEELGKETQAGNQTTEGQQQGNQ
                     TTEGQQQGNQSDNPLSNVGKAIGDLFK"
     gene            4013..4318
                     /locus_tag="VF222_07265"
     CDS             4013..4318
                     /locus_tag="VF222_07265"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142261.1"
                     /translation="MNPKYRSRIEIISSILEASVEGAKKSKVMHRSFVSNTQFKLYMN
                     HLIEKNLLSIDSESKLTITPKGLEFLDHYHTLESMLDLTIQKQSIKNDVYSQYLLPT"
     gene            complement(4577..4993)
                     /locus_tag="VF222_07270"
     CDS             complement(4577..4993)
                     /locus_tag="VF222_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142262.1"
                     /translation="MGNEGGTSGPTILWDEIKDKKVKSVDGEKLGRIEKISQNHLMIE
                     EGFLKKKRFWLPKFLADVYDGNHLWLDINKEEIKKRYYYDEEPASSEYDLNMSEFNTK
                     YGKSKSNASNDKIRLKEGTELKTKSKKGYKNIRDLK"
     gene            5107..6099
                     /locus_tag="VF222_07275"
     CDS             5107..6099
                     /locus_tag="VF222_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142263.1"
                     /translation="MTVGLSWDKLKGKNAKSIDNKEIGKIKEISQDYIQIEKGIVSKD
                     HFFVPKYFVEGYEDDDIIISLTEDEIKNKFMNKSNPSGDMLEDENYKKRKEFLKKKHP
                     NYENDIPEYYPVASGEDQDSTRGQGAQMSWDKVMEKEVKSSDDEEIGKIKSVGQYFVE
                     VEDGVISKKHYFIPKKYLQHYDGDTIYSSLEKEEIKQKYQRDSPPLETELQDIEKGDD
                     FQLIPFMAKEPGVEIKTQTMEEELKVPWEEIIHKHVRTTDNIDIGDVDKVGNEFIVVR
                     EGFANVHLYYIPKKYIIKYDGSSLWLNLSSTVASSKFERKNEPSAEEMEMLLNQ"
     gene            6572..7417
                     /locus_tag="VF222_07280"
     CDS             6572..7417
                     /locus_tag="VF222_07280"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7142264.1"
                     /translation="MVRHTKFSSYDGKTSLFSLPNTIKLKNPLLIAGFPGPGLVGSIS
                     INYIIKKLDMQQIFCFESEFIVPGVIFMDGKLHHPFRIYSNVDGTICALVCEAPIMIN
                     GMHSVLDALGKWAEDFGINDVFVIDGMAIRPVSNTEVNSQPTAKTERSPIILSSDESK
                     IIHSNSNKKNLNIESLIEGSTTFIGGIAGGLLSSSLSHNIHCSVIFIPTYSGIPDPDG
                     AALTIELLQKTIHNNNFKIDTAELRKEGEDMKHKLNNLIKSIKQQQEQSQLIQDVKHD
                     PIMYS"
     gene            complement(7404..7592)
                     /locus_tag="VF222_07285"
     CDS             complement(7404..7592)
                     /locus_tag="VF222_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142265.1"
                     /translation="MIYPSKGLFRGFISSSSDYGKTEVDHEHRMYAKKNNLNLHKIIT
                     YTFIQQSEKGDMQFIKNT"
     gene            7833..8030
                     /locus_tag="VF222_07290"
     CDS             7833..8030
                     /locus_tag="VF222_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142266.1"
                     /translation="MAKDNRKITNRGLASANDETRVRVAREGGKAPHNERGLQATDNE
                     TRVRVAREGGKASHSGGRKSG"
     gene            complement(8324..8758)
                     /locus_tag="VF222_07295"
     CDS             complement(8324..8758)
                     /locus_tag="VF222_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142267.1"
                     /translation="MENKNFINESTSSPPNNGITAAEQLKSSYKQFLAESAKLFPKVD
                     QKLLLDMIYLETTYKDWEGSVLLKIVYSSHSQVDTNKKKEEIYNKYQKMPDEVIEGRT
                     LRVKLIRMYIKNIEELITSDTDIEFITGSATLAPSDSYSDSS"
     gene            complement(8770..9021)
                     /locus_tag="VF222_07300"
     CDS             complement(8770..9021)
                     /locus_tag="VF222_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142268.1"
                     /translation="MESERNNIVVEDWQAVVSKPVYSSNGKDIGIVRAVQPEFLITSF
                     GSITQDRYLIPKTSIQSFTNGIIYINEDSDFVEKNFKFE"
     gene            9491..9856
                     /locus_tag="VF222_07305"
     CDS             9491..9856
                     /locus_tag="VF222_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142269.1"
                     /translation="MTFNETCFTDTEIYLNYEEHITLLPKMVSNTKKYKKTSKSNSNS
                     IELYDLIAIKFPGEGQILSGFITIKYRYNLKNGIITYSDIKYSDKRMERYLEEQPNVI
                     KNIDSYLKNKMLESELKSI"
     gene            complement(9900..10250)
                     /locus_tag="VF222_07310"
     CDS             complement(9900..10250)
                     /locus_tag="VF222_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142270.1"
                     /translation="MIIDDEIDITNLFKEILTNAGYHVEGFNDPLKALAECNINHDKY
                     VLIISDVRMPKMTGIELVKKIAEIDDNIKVILMTAFDVTGEELKEVEIEKFLNKPVHL
                     KKLVDIVKMSLSDE"
     gene            complement(10662..11057)
                     /locus_tag="VF222_07315"
     CDS             complement(10662..11057)
                     /locus_tag="VF222_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142271.1"
                     /translation="MIPYEFIYSFTNTVLHLDESIRWVGLTNREGLILNEKYREKLHP
                     LLTEEENEEYASNAISRQRTRSKFEQKIGKLIYAFGKYEKLNRATIPINGNYFLLLSI
                     DSDELNFDKIIMDKLLPLISETKEQFVSI"
BASE COUNT         4074 a         1654 c         1520 g         4137 t
ORIGIN      
        1 tactttatag catattccaa tgctagcaac cctccaaatg atgaacctat tataatagga
       61 tcagatacat gaagtgtatc tagaaatttt tttaagaaat taataaaaaa atctactgta
      121 tattcaacat gtggtttttc actatagcca aatccaataa tatcaggaat aattaatcta
      181 tattttgagg acagagatgg aatgattttt gtccatctgt cagcagatcc tcccaatcca
      241 tgtaaaagaa gaatggtcgg tcgatctgaa ctacaattaa agatatcgta ataccgaatt
      301 aaatatttat ccacctctac attcttactg aaatgagcat taaccaaatg tcatgattat
      361 tgatatggac tgttatttaa ttccaatgtt aaagcaacca taccaaatat ttaaaaattg
      421 tagaattttt aattttcctt ttagataatt agtccaatta aacataccga tagattcagg
      481 aaatagtata ttatatcata attataatta attaccattt gttgtatcca taaagtttac
      541 tactattagt atttaattta agataggcga atattttctc tatagctaat ttcaatgacg
      601 acatctctgt accaaaagga taagttctct aaccatcatg tactcctgta tttaaccagt
      661 ggcactaacg ggtttcagca atgaattcct cttcgaataa taaagaaatg ctatgatcac
      721 atttatcaga cttcatgaca ttaatctctt tacaaattat attttatttt cggttcaaaa
      781 taatagtcat agtaaatatt taccgttggt ccatgactct attcgggttt ttgattatta
      841 ttggagattt atatatctct atcctcccat ttacaaatct acgttatatt atatcttatt
      901 aaatatagtt tctaaacatg aattacagga ggattaatta aaattacaat tctaattatg
      961 atttgcccat gtggatacta tgaaaaaatt attctttacc aatacgaata gatagaagtt
     1021 tgattatttc tttgtaacct tattgtctag gcctaataca ataagatcat gaatttacct
     1081 aggacaattt gaatgttgag aatttataat gaatttcttc taaatatcct atggccactt
     1141 agctagagaa tatttcatga cctcttgtct agttgtattt ataaaatcaa ttagtttttc
     1201 actcagtttt tctcctgcga agacattcca aagtaactta attaaattta ttgcatttac
     1261 caataaggtt caaagaaata ataaaaaata tctaactaat ttctattgat tatattgtta
     1321 ttttttagtt gaggggacga tatgacgagc tccttttcta atatattttg gattattgtt
     1381 tttaaagtac ttttaaagtt gtttactgaa aaaatatcag gatatttact tatttttaat
     1441 ctatcctcaa atgatatttt cattgaatca attattattt ttactgcttc ttcttcggtt
     1501 ttaaaatgga atcgagatgg aacaaattca gtattacctc ctttgaatgg gacaacggga
     1561 attaatccag cactcattcc ctctgctaca gacataccaa aaggttcctc tagagttgga
     1621 tgtaataaaa ttttagaagt tgccatcatt tttaacacac tatcaagggg aacgtttaac
     1681 tgtattttta catgttcatt tagtctatac atgcttatta gagattgcaa atatctgtaa
     1741 tagtcatagt tatctttaac tatattccca ataagaatca ttttaaaatt cttaaagaat
     1801 ttgtgtaaga ttcgagctat tcttatggct gtttctattt ttttatcagg actataccta
     1861 gaaataacaa ttattgtatt gtctcttatt agattactca aagcaacctt tcgataggtc
     1921 tctacatcaa caggtggact taatatagat aagaccactt tgccatagta attttctatt
     1981 gcttgcttgc taaattgaga gtttgttaca attaaggtat ttttcatcat aagatcatat
     2041 gttgataata ttctgtatag taattctttt tctttcacat gggagtttcg ttcataatca
     2101 actatactat tctgtaaaaa tttcattaaa aattttttat aagaaccatg attgatcaac
     2161 ctaggagtta gaggataatg acaataggta attatctttg tttttaattt tctatggcta
     2221 gtatagttat aatatggtaa gatatctcca tgagtattta ttattaaagt atatttttct
     2281 tcttctaatg ctatttgttt cttcttttca tttgcgcatt cagaagaacc aaaaacattt
     2341 gaaagatcta ttttttttat tttttcgatt cttaatgaaa attttccata ccacttttta
     2401 atttgattcc aatcaggagt tgatatagtt tctaaatcaa tattaaaacc catttcattt
     2461 aatgcctcca tcatagttat ggttaggcgt tcacttccgc caccagcatt taggtcttca
     2521 tgtataattt taatattgtt tttcatgata ttagtaatac aatataatat attaactaca
     2581 attttttaat accaaataat taacgtatta attattgtac tattatacaa tgtttctttc
     2641 tatggtcaat aatgctaaat aacaaatgta agaaaatctt tccaaatata aatttaggtt
     2701 aactcataat agttgaaggt cattttgtat tgactatatc ctcttctgat ttaaattata
     2761 acaaccttta ttcatacctc atgacctgaa tgaagttaat aaattacaat tgaggaatat
     2821 gttttcattg gtcagcattg aatatttctt tttatattgt acaaacatac tttttcttgt
     2881 accctaatgc agagctcgta taggttgctt attataaaat aaattaacta caatataaaa
     2941 atatttctta tggtaattgt tttatagttt atatcactaa gattatcatg aaaaatagct
     3001 ctagaataaa ctatattttt ctcttattat cctttggaat agtatctact atcttgtcta
     3061 gtccgatatc agcccaaaca caaaacaatt cacaaaatca atcagcggga acagctagtt
     3121 ctggccctta tgctacggga aaaccaggaa ttggaagtgc ggatgaactg gagagtataa
     3181 ctaatgatac tcaaactagt actagtaatg acactactcc tatgatggaa gaattaggga
     3241 aagaaactca agctggtaat caaacaacag aaggacaaca acaaggtaat caaacaacag
     3301 aaggacaaca acaaggtaat cagtcagata atcctctcag taatgtagga aaagcaatag
     3361 gagacttatt caaataattt tttttcttaa acaatttttt atcataaata gctccaattt
     3421 tggattctca aagatattta ttgtatactc aataccgcag gctctgagct taatatatta
     3481 taattgtgac acattatcaa ctactaattc atatattcaa atataattaa tagattattt
     3541 ttcatcagaa aaaatctttc ttttcataac cacaacaata aacccccaac ctttattgtt
     3601 gactttttac acacgtttca tctcttttaa ttcattatta taaatctaat agtaattgta
     3661 agaattaaaa tattaacctc ctttcttaaa tctttttcta taaagatgtt aaaaactacc
     3721 tctattacca tttcacacct ttgattttat aaaatagata tacaatcaga tctcaaatta
     3781 taatatttta atgagataga acataattat aaacttaaaa atatgaatat agattgaact
     3841 agaaaaatta agtatagaac tatcataaca taatcaatag attagtattt tctacaatta
     3901 atacaatacg tcatagaata tctttagctt cataattata gaactttgag tcttagaaat
     3961 tagatttacc atattcataa ttcattatat actttcgtgt taataattaa ttgtgaatcc
     4021 aaaatataga agtagaatag agataatttc ttcaatacta gaagcatctg tagaaggagc
     4081 aaaaaaatca aaagtcatgc atagatcgtt tgtttccaat acccagttta aattatatat
     4141 gaatcattta attgagaaaa atttacttag tattgattcg gaaagtaaat taacaattac
     4201 acctaaaggt ttagaatttt tagatcacta tcatacttta gaaagcatgc tcgaccttac
     4261 gatacaaaaa caatctataa aaaatgacgt ttactcacaa tatcttctac ccacgtaatt
     4321 tctgacgata tctattgtca tccgtttttt ttagaaataa tgtaatgata attcacctat
     4381 cagaggaaaa taaaattgca aataaataat aaaaacagct tcttgaagaa aatgaattca
     4441 tgaattatca tatcaactta gatcagtcaa tatataatac aatatctctt ttatagaaga
     4501 attcatgaag aattaagaac gattattgtg tttacaataa ttgtacaagt ggctaaagtt
     4561 taacctctgc ccaaatttat ttcaaatcgc gtatattctt atagcccttc ttagatttag
     4621 tctttaattc cgtgccctcc tttaatctta ttttatcatt cgaagcattg ctcttacttt
     4681 taccgtactt tgtattgaat tcgctcatat tcaagtcata ttccgatgat gctggttctt
     4741 catcataata atatcgcttt ttgatttctt ctttgttaat atctagccat aaatggtttc
     4801 catcatatac atcagccaaa aatttaggaa gccagaacct tttctttttt agaaaacctt
     4861 cttcaatcat taagtgattt tgagatattt tttctattct tcccagcttt tcaccgtcaa
     4921 cagatttaac ctttttatcc ttgatttcat cccaaaggat agtaggacct gatgtacctc
     4981 cctcattccc cataatacct taattgatca acatgaaata taaagaatat attgatttgt
     5041 ttccagagtt ttggttttat taataaacta ataaattata tgttgtatag tttactattg
     5101 agaaaattga cagttggtct ttcgtgggat aaattaaaag gtaaaaatgc caaatctata
     5161 gataataagg aaattggaaa gataaaagaa atatctcaag attatataca gatagaaaaa
     5221 ggcatagtaa gcaaagatca ttttttcgtt cctaaatatt ttgttgaagg atatgaggat
     5281 gatgatatta ttatctcttt aacagaagat gaaataaaaa ataaatttat gaataaatcc
     5341 aatccttcag gtgatatgtt ggaggatgaa aattataaaa aaagaaaaga atttttgaag
     5401 aaaaaacatc ctaattatga aaacgatata cctgaatatt accctgttgc atcaggagag
     5461 gatcaagatt ctactagagg tcaaggagct caaatgagtt gggataaagt aatggagaag
     5521 gaagtcaaat cctccgacga cgaagaaata gggaaaataa aaagtgtcgg tcaatatttt
     5581 gttgaggttg aagatggggt aattagcaaa aagcattatt tcatccctaa aaagtatttg
     5641 cagcattatg atggtgatac aatatattct tcattagaaa aggaagagat aaagcaaaaa
     5701 tatcaaagag attcacctcc tctcgaaaca gagttacaag atatagagaa aggagatgat
     5761 tttcagttaa ttccttttat ggcaaaagaa ccaggtgttg agataaaaac acaaacgatg
     5821 gaggaggaat taaaagtccc atgggaagaa ataattcaca agcatgtgag aaccactgat
     5881 aatatagata taggagatgt agataaagtc gggaatgaat ttattgtggt tagggaagga
     5941 tttgcaaatg ttcatctcta ctacattccg aagaaatata ttataaaata cgatggtagc
     6001 tctttgtggt taaatctttc atcgacagtt gcgtcatcta aatttgagag aaagaatgaa
     6061 ccatccgcag aagagatgga aatgctgctt aatcaataag ttttatagaa ggatgaaaat
     6121 atagcattct acaagtttca cctatatttt ttacttaatc gcttatcgtg tcctaataaa
     6181 gataatagcg attttaatgt cctaactata ttattaattt cctgcaaccg ttgctttgca
     6241 acatgtatta aaaaaaaggt tcattcaaag attcgttttg tttatgaaga ctaaaaacaa
     6301 ttatgtggtc atctaattag tataggtcac aattacaaaa ttttttttcg ctaataaata
     6361 taattacttg tatttgttta atgacaatta agatactcta tcctatttga aattttaata
     6421 ttgaaagttt tactgatata ttcactagtt tctcttattc aatcacttta aaagtaaagg
     6481 ctagattgtc aaatataaag tgacatttaa ttaagatctc tcccgcttac tattaaatag
     6541 tatgtatcac atttttatat agaatattta aatggtacga catactaaat tttcttctta
     6601 tgatggaaaa acttccttat tttcattacc taatacaata aaattaaaga atcctttact
     6661 tattgctgga tttcctggcc ctggtttggt cggttcaata agtattaatt atatcattaa
     6721 aaaattagac atgcaacaaa ttttttgttt cgagtcggaa tttatagttc caggcgttat
     6781 ttttatggat ggtaaattac atcatccttt taggatatac tcaaatgtag atggcacaat
     6841 ttgtgcactt gtatgtgaag ctcctataat gatcaatgga atgcattcag tactagatgc
     6901 tcttggtaag tgggctgaag attttggtat taatgatgtt tttgttattg atggaatggc
     6961 tataagacca gtctctaata cggaagttaa ttctcagcct acagccaaga ccgaacgtag
     7021 tcctattatt ctctcaagtg atgaaagtaa aataatccat tccaatagta acaaaaagaa
     7081 tttaaatatt gagtctttaa tagaaggctc aactactttc ataggaggta tcgccggggg
     7141 tttattatct tcctctctct cacataatat tcattgttca gtaattttta tccctactta
     7201 ctcaggaatc ccagatccag atggtgctgc attgacaatt gaattattac aaaaaacaat
     7261 ccataataac aactttaaaa tagacacagc cgaattgaga aaagaaggtg aagatatgaa
     7321 gcataagtta aataacttaa tcaaatccat caaacaacaa caagaacaat cccagttaat
     7381 tcaggatgta aagcatgatc ctattatgta ttcttaatga attgcatatc tcctttttcc
     7441 gattgttgta taaatgtata agtaattatt ttgtgtaggt ttaaattatt ttttttagca
     7501 tacattctat gttcatgatc aacttctgtt ttcccataat cactgctaga actaatgaaa
     7561 ccacgaaata atcccttaga tggataaatc attaccatat agtctatgaa tctttttcaa
     7621 caaaatcatt atccttaggt gatcgaagat catctctcta gtttttgctt gaaattcttg
     7681 tattaattgg gtcttaatcc accttgcagt aattcttact acattctcaa taattatgat
     7741 gttgtcatta agatcataac atttaattca ttagaaaaaa tttctataat ttatctataa
     7801 ccttttaata aattgatgct taatcaaagg caatggctaa agataatagg aaaattacta
     7861 atagaggatt ggcatcagcc aatgacgaaa caagagtaag agttgcgcga gaaggtggta
     7921 aagctcctca taatgagaga ggattacaag ctaccgataa cgaaacaaga gtaagagttg
     7981 cgcgagaagg tggtaaagcc tctcatagtg gtggaagaaa aagcggttaa actgaattct
     8041 taaagacaaa taatgtaatt aaatataact cattaattat cataaatatg tttttttaaa
     8101 aatttttatt gagatattat gatataaatc taagtatgca aataaataac ccattcctat
     8161 cctggtaaaa tagttgataa ttattggtat tcttttatac catatttaat aaaatttctt
     8221 ccatcttcgc tgtataataa gaatttataa tgggtatatc tttgaggaca aatataaaat
     8281 aggtcgttga ataattatag acctaagtta attgaattac aatttatgat gaatcggaat
     8341 aggaatctga tggagccagg gtagctgacc cagtaatgaa ctcaatatca gtatcactag
     8401 taatcaattc ctctatattt ttaatgtaca ttcttatgag tttaaccctc aatgttctac
     8461 cctctattac ctcatcaggc attttttgat acttgttata tatttcctct ttctttttgt
     8521 ttgtatccac ttgagaatgt gaagaatata caatctttaa taaaacacta ccctcccaat
     8581 ccttatatgt agtttcaaga tatatcatat ctagtaataa tttttgatca actttaggaa
     8641 ataatttcgc agattctgct aagaattgtt tgtaagaaga tttaagttgt tctgcggcag
     8701 tgataccatt atttggagga gaagaagtag actcattaat gaaattctta ttttccattt
     8761 ggtatcatct tattcaaatt taaaattctt ttctacaaaa tccgagtctt cattaatata
     8821 gataattcca tttgtaaatg attggataga agtcttaggg ataagatacc tatcctgtgt
     8881 tattgagcca aaggaagtaa ttaaaaattc tggctgcacc gctctaacaa ttccaatatc
     8941 ttttccatta ctcgaataaa caggtttgct cactaccgct tgccaatcct caacaactat
     9001 attatttctc tctgattcca taaattaagc tagacaatat aaaatttaaa tgtttgaata
     9061 tatattatct ataatattca cgtttagaat catttaattc tagtaatgtt gggttgaaat
     9121 aatcacatcc ctgattgtaa attctagtat aaaaaaattt atcaacataa taaatatata
     9181 tcaaaatttt aaattgatca ttcgagtata taaagcatag aacgattgaa tgatttttta
     9241 attgacaaaa cgattaaaga attaatgact ctatttataa tttataatat taaatgtgag
     9301 aaatatttag ttttgatata tgtttatttt ttagactact taattgctat tatagaatgg
     9361 tattaaagag aatagagtca attgtaatga ttagatgttt aaccttcata aatctttaca
     9421 gaacgtttgg tataagtaca atttatctta gctgttaaat agtttatatg gctaatataa
     9481 agtattgagt atgacattta atgaaacttg ttttacagat actgagatct atttaaatta
     9541 tgaagagcat ataacattac tcccaaaaat ggtgtctaat actaaaaagt ataaaaagac
     9601 atccaaatct aattctaatt caattgagct ttatgattta attgctataa aatttcccgg
     9661 tgaaggtcag attctctcgg gatttataac aattaaatat agatataatt tgaaaaatgg
     9721 aataataact tattctgata taaaatatag cgacaaaaga atggagagat accttgaaga
     9781 acaacctaat gttataaaaa atatagattc gtatttaaaa aataagatgt tagaatcaga
     9841 attaaaatcc atttaatttc ttattgcagg tatacaattg agacaacgtt tgttagccct
     9901 tattcgtctg acaaagacat tttaactata tcaactagtt tctttaaatg aaccggttta
     9961 ttcaaaaatt tttcaatttc aacttctttt aattcttctc ccgtgacatc aaaagcagtc
    10021 attaagatta cttttatgtt atcatcaatt tcagctattt tttttaccaa ttctattcca
    10081 gtcattttag gcattcggac atctgaaatt atcaagacat atttatcatg atttatattg
    10141 cactctgcta atgcttttaa aggatcatta aaaccttcca catgatatcc ggcattagtt
    10201 aaaatttctt taaataaatt agtgatatca atctcatcat cgataatcat gattttttgt
    10261 ctcgacaata ttaatactga aaacgataat atgattaaca gtataattag ttattgcttg
    10321 aattaattta ttttttgacg atctataata atcacaatat taaaaaacga taattattaa
    10381 tgctatgtga tataacaata ttaaattaaa gaaattctta ttgcttgctt aaaataatta
    10441 gtaactttct ccatggttct tttaaaatag acaataataa tccatattaa gccaaccaac
    10501 tataatattt caaattaaat ttattcaatt aatataattc acttgtggtg tgaaatttat
    10561 ctagattact tatatatatg ctgattgatt acttttattt cttatattta aaaaattatt
    10621 tttaatattt attactatac aatacctgtt aatttttttt attaaataga tacaaattgt
    10681 tccttagttt cactaattaa aggtagaagt ttatccataa ttattttatc aaaatttagt
    10741 tcgtctgaat ctatacttaa aagcaaaaag taattaccgt ttataggaat agtagctcta
    10801 ttaagttttt catatttccc aaatgcatat attaatttcc ctattttttg ctcaaatttt
    10861 gatctggttc tttgtcttga aattgcatta gaagcatatt cctcattctc ttcttccgtt
    10921 aggagaggat gtagcttttc acggtatttt tcattaagaa ttaaaccctc cctattagtt
    10981 aaaccaaccc aacgaattga ctcatcaaga tgtaatacgg tatttgtaaa tgaataaata
    11041 aattcatagg gtatcatcta taattatctt cataagcaaa taaaaaaagt ttgttaataa
    11101 aacacatatc ttgtatagca cttaatacaa aattgttaat atttagaaac atgatatcat
    11161 ccatcattaa tttatcattt attaaaagat ccttaattga cacttagtaa aatgaattta
    11221 tcttactaca tatcagacct agaaaacaac cattttccta ctcttggaag tacatttagt
    11281 tgtgaatagc tactagcaat taatccaaca tgtccagttg gaaatttcat taatctattg
    11341 tcgatacttg agacgaggta gtttagtgcc gcactacctt ctgga
//
LOCUS       DASVEV010000159        11242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_57570, whole genome shotgun sequence.
ACCESSION   DASVEV010000159 DASVEV010000000
VERSION     DASVEV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57570"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1121
                     /locus_tag="VF222_07320"
     CDS             351..1121
                     /locus_tag="VF222_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger domain-containing protein"
                     /protein_id="HEX7142272.1"
                     /translation="MLLRVGYMRCYVCNGEDFLFKCNYCKEFFCTDHRLPINHFCRAI
                     DKFSEKRKTNLFPHNSIKSSFLDDFSKTINIRFSRTEIKHLLLATFLVLLVGMSLNNY
                     RYISFEFLVIFIIAFLFHELAHKFLANFYGSWAEFRAELYGLIVTAISAIPFIPFKFI
                     APGAVIVGISDNEKLGKVALIGPMTNLVMGFSFLLLSYLYPFSIYFSIGASFNAWISM
                     FNLLPFGVLDGQKIFTWNKVVWSFTMAITMILFVISYS"
     gene            complement(2130..2285)
                     /locus_tag="VF222_07325"
     CDS             complement(2130..2285)
                     /locus_tag="VF222_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142273.1"
                     /translation="MKHIEYIKKCARNLSQEFNMERKVIAFHLITLEKAGFVNSEFGI
                     IIKDLLL"
     gene            complement(2389..3513)
                     /locus_tag="VF222_07330"
     CDS             complement(2389..3513)
                     /locus_tag="VF222_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7142274.1"
                     /translation="MKFDSNQILNRIGKKKVSDFIESANIFNSNENISKILGTLIKIK
                     DYSVYFKNGNKIIAINLRDILSAREVHSQKLNSIAKSTPTIEEDSNIRRAAGLMSLYR
                     LRSLPVIEDGEIIGQITTKSIVKSMEHTDIKIRSTKIMTANPISIHENDLVLTAKNLM
                     IRHRIDHVPIVEDSLKGIITSFDIAKILLTADNNDNVYRGLTEHTRPLEFPAKGIADK
                     NTTTSSVDDSIAKVIHLLNSNNSTYSIVTLGNEIQGIITHRDIISLLGEKVEEDVPAY
                     IIGLPDDPFASELAKTKFNALVKFLKKTMPDLEEARCRIRLSTVRGKTKKYEIEVSIF
                     TPTNRYSYRNEGSDLATIFDQMKDSLKNKLTQKNQKHQRK"
     gene            4395..5366
                     /locus_tag="VF222_07335"
     CDS             4395..5366
                     /locus_tag="VF222_07335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7142275.1"
                     /translation="MSCIILPKSEKSSQLATKYKFKEWMIARYLTIVPETEKFLDYIN
                     NRVSNQYIRINTLKIDIDTIKKKLSEKDIKLEKTLLSEVFLIKESKYPIGATMEYLSG
                     YYYIQDLSSCLAVESLDIQDDDMVLDMAASPGGKTSFMAQKMNNRGTILALESERKRL
                     RKMMFNLSRCGVINTVLWNLKGEKIINYDLKFDKVLLDAPCSCDGIMQKDPIRKTTYS
                     KKTIQYCSLMQSQLIESALKVVKPNGLLVYSTCSLAPEENEFIINSMIDKFDITIESI
                     NYGVDSLLQFGGKIMDKSIQFTKRFYPHIHNTAGFFIAKIRLNNIAK"
     gene            5356..5895
                     /locus_tag="VF222_07340"
     CDS             5356..5895
                     /locus_tag="VF222_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142276.1"
                     /translation="MRNDMLRELTRQEKTIILREFNRWGIFNYLHDKEFAVEACENNL
                     KCLFIIPVVLKSLIFYPYTCYGGLPIGNLQKKKFIPSIAFFDIVAKNSNSFLYVIVDS
                     NGEKLILYGRDIFGNSILYASPNIEENSILLILNKSKELLGIGRSRFHITKIKENGKV
                     TINTLMDIGTYLRSENNQK"
     gene            complement(6199..6762)
                     /locus_tag="VF222_07345"
     CDS             complement(6199..6762)
                     /locus_tag="VF222_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142277.1"
                     /translation="MNIQKNGKSDNGILFIFISLSVFSATILSYIIHLKLDFLLMDII
                     IWIGCFFIYFIIFRRKIILSLHIIRNKIRKSTAWSPSFKLINGLCWAAPFGLGALIPS
                     IHEYLILAGIGLGNLSTFFIFMVKNNLKNLDQFIVGLISIISIGIIILLYNENIIDKS
                     SGDFLARILISVAYGIGGIYSSMIKSE"
     gene            6919..8232
                     /locus_tag="VF222_07350"
     CDS             6919..8232
                     /locus_tag="VF222_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142278.1"
                     /translation="MSPFFNIFKKKSEKERIKPKYPNTIDQIRTRETEERRTISIVEA
                     KDIIKKIDLSQTKILTGRINKIRHLTQKPIGNIKALISDLESTKIDVHESSFESVVVN
                     SKKMVINSIRKELSSNIPSPATLNDVIKLHERLDSMVNRFSEVSKSHKKVFNFFISKY
                     ADKLKTEFENISSLSMQCKYEIDNFEKERQPLQNCFDNIDALIQKIELIKLEEEKFKT
                     KQFQLDEMKIKIKKLNIEIKNLTDSKKYLESKDVVIKIQSLDLEKDNLHKELINAFSK
                     VSRAMNKYSYGLNKLIVQRIEIMTEQPWKIFDDLITYLNLIKEVKNAISKGKIIVKES
                     EKIESYLDNIINALPNYQEKEEKLNSEIKKLNSNKNMHVISKLNELKRKNASYNQELQ
                     NIELYLKDSESEVAKIKDEYEDLVVSIETYINQITKSKYHLSISN"
     gene            8627..8857
                     /locus_tag="VF222_07355"
     CDS             8627..8857
                     /locus_tag="VF222_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142279.1"
                     /translation="MNNFSKPVKIGVTILLIGVAVMAYGTYLNSDTISYYALLIIITG
                     LFIYLAYTFNFVSKSKVDHTTTEKEKGKKRKK"
     gene            9712..10350
                     /locus_tag="VF222_07360"
     CDS             9712..10350
                     /locus_tag="VF222_07360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX7142280.1"
                     /translation="MRVYIAATIIGIVLLMAIITFHADEAFAKKGDSSSKKKIELCCA
                     WGKALDDGILTFSIKNGGSDLAKIVKLAINDWEKALGGVDFKYVKDDTDADIEIDFKK
                     GKGKKVGKTVTFFDHLGLIAQVDISISKKSYGFTLDKQTLEHVAKHEMGHALGLGHAT
                     FQGTLMSPNVDEIITKISACELGSVKYANSWKFNGGKSPTSLDKDNFKCKKK"
     gene            10508..10861
                     /locus_tag="VF222_07365"
     CDS             10508..10861
                     /locus_tag="VF222_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142281.1"
                     /translation="MENNSLTKDIMNLDKKVRFAAVCDDSGEIKYGGQREGIPNLLSP
                     EETKRSNIQALARWSLRNSLASKIGKGQYAMAVYEKIKRITIPLENDHLLLVTTEIDA
                     DHEKIISEVMSLLKK"
     gene            complement(10930..>11242)
                     /locus_tag="VF222_07370"
     CDS             complement(10930..>11242)
                     /locus_tag="VF222_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142282.1"
                     /translation="LVNDLLSRQQKDPDSAPFYMVEMFTEDGTDEEAKREFIIRKTGM
                     APAIYDNGTHYVTNQRLTLEILNELAEPEDVIKITGDFTGNLGGVGASHSRREATETG
                     E"
BASE COUNT         4164 a         1497 c         1577 g         4004 t
ORIGIN      
        1 atattatgtg cttgaaacgc ttttctattg ctttttcatc tgccatacct agccaaacat
       61 gtaaactaga ggttttacaa atggtctatg aggaacaaat caatattgtc aggttcattt
      121 aatttcggtc aaatcaaacc atgcaaaatg gtttctaatc ctaactcgtt tcatttttgg
      181 cttctaaaaa tgccaatagt ctaatagcga ttagttctgt tctgaaattt ttcaagattg
      241 gtattttcca tgtttgatgt tgtttaaact aaattaaata cttcttaaat ttttcattta
      301 tttatacata tacaaggtta ttcagtattt ctcagcggca ctcatatatc ttgcttctta
      361 gagtaggata tatgagatgt tatgtatgta atggagagga ttttctcttt aaatgtaatt
      421 attgtaaaga atttttttgc acagatcatc gtcttccaat taatcatttt tgtagggcga
      481 ttgacaaatt ttctgaaaaa agaaaaacca acctatttcc tcataattcc ataaaaagta
      541 gtttccttga tgattttagt aaaactatca atatccggtt ctctcgaacc gaaataaaac
      601 acctacttct agcaactttt ttggtattgt tagtaggaat gtcattgaat aattaccggt
      661 atatctcctt tgaatttttg gttattttta taatagcatt tctttttcat gaactagccc
      721 acaaattttt agctaatttc tatggaagtt gggctgagtt tcgtgcagaa ctatatggtt
      781 taattgttac agcaatatct gctattccat tcataccgtt taaatttatt gcgccaggag
      841 cagttattgt tgggatttca gataacgaaa agcttggaaa agttgctctg ataggaccta
      901 tgacgaattt agtaatggga ttttcatttc ttttgctttc atatttgtat cctttttcaa
      961 tttactttag tattggagca agttttaacg cctggatctc tatgtttaat ttactaccat
     1021 ttggagtatt agatggacaa aaaattttta cttggaataa agtagtatgg tctttcacaa
     1081 tggccataac tatgatttta ttcgtaatat cttattctta atgaatttat atgtaatatt
     1141 atataaaaaa aaataatagc gaagatttat tttaagatcc tgtattaatt aaatttaaaa
     1201 aagatacata atacttgttt aaatttttat gtattataac tatattatga gtcgggagtt
     1261 tagaaataca aattacgaga aatatttaaa agataaaaat aaaatattac attttcattc
     1321 caaagaatat aatagtttta agagatcaat cttcaaatca agacaatatt tatatttaca
     1381 atagataaaa acatcatata gcgataaatt tgttaataga acatctattc atattctttt
     1441 tgtcaatcca tttcattgta accaaactct taaggattga agttagtaat ctttaaagtt
     1501 ttacaatata accgaaggaa tgagatgttt atacgaggag tcgaaaaggt cataattgtt
     1561 tgtggacctt gatagtaact atttaattgc tttaaaattt tctaattatc tatttgtgaa
     1621 atgtatcagt ataagttgag aattttatat gcatgactca taaagataac ggattggaaa
     1681 aataaaaatg gaatccaaaa aatgttaaaa ctttaatctt ctatagccaa agtgttttaa
     1741 tcactttagg gaaaaccaat tgtaatacct tatttgtcat gcataagcgt atagttgtat
     1801 tatacctaaa ataaaaagga attaattcaa ttctagttat ctaaaactgg tacaagttct
     1861 aagtgacagc tagagcaatt gtaattcttt ggattttttt catcaacatt aattaccatg
     1921 tattctttac atgttctaca atacattata gatcctgtag aagaaaaatc cattacactg
     1981 tataggtgaa acaagctatt aaatatgcct attttgggtt ctgtatactt accttatcca
     2041 taaaaaagtg ttaattgtcg tctcatttct ttaaaatctt tcagatattt caaatatctc
     2101 tttatctttt ggagtaatct cttaatactt tacagcagaa ggtcttttat tattatacca
     2161 aattcactgt ttacaaatcc tgccttttct aaagtaataa ggtgaaatgc tattactttt
     2221 ctctccatgt taaattcctg tgatagattc ctagcacatt ttttgatata ctctatatgt
     2281 ttcactttaa ttatttatcc taaaacgtac aatagcaata gttgaaaatg taaaggcgga
     2341 gaataagtaa aaaaagtata tactatactg tcagtagttc tatttttttt atttcctttg
     2401 atgtttttga ttcttttggg ttagtttgtt ttttaaacta tccttcatct ggtcaaaaat
     2461 tgtagcaagg tctgaacctt cattcctgta actatatcta ttagtgggtg tgaatatact
     2521 tacttctatc tcgtattttt tagttttacc tcggacagtt gataatctta ttctacatct
     2581 agcttcttcc aaatctggca ttgtcttttt aaggaattta acaagtgcat taaatttcgt
     2641 ttttgctagt tcagatgcaa atggatcatc aggtagacca attatataag ccgggacatc
     2701 ttcttctacc ttttctccaa gtaatgaaat tatgtctcga tgtgttatta taccttgaat
     2761 ttcattgcct aatgtaacaa tagagtaagt tgaattattg gagttgagca aatggataac
     2821 cttggcaatc gagtcatcta cactcgaggt agttgtattc ttatcagcaa tacccttagc
     2881 aggaaattcc agtggtcttg tatgttctgt aagaccacga tacacgttat cattattatc
     2941 tgctgtaaga agtatttttg caatatcgaa tgaagtaata attcctttca agctgtcttc
     3001 tactataggt acatgatcaa tcctatgtcg tatcattaga tttttagctg tcaatactaa
     3061 atcattctca tgtatgctaa ttggattcgc agtcattatt tttgtagatc taatcttaat
     3121 atctgtatgt tccatagatt taacaattga ttttgtagtg atttgtccta ttatttctcc
     3181 atcttcaatt acaggaagag atcgcaatct gtaaaggctc atcagccctg cagctcttct
     3241 aatattgcta tcttcttcaa ttgtaggagt actttttgca atagagttta atttttgaga
     3301 atgaacttct ctagcggaaa ggatgtctct taaattaata gctattattt tattcccatt
     3361 tttaaaatat acactataat ctttaatttt tataagagtt ccaagaattt tagagatatt
     3421 ttcatttgaa ttaaatatat tggcagattc aataaaatct gaaacttttt ttttgccaat
     3481 tctgtttaat atttggtttg aatcaaattt cattaattag ttagtttgag gtataaatat
     3541 taatagattc ttatttgaat gattaatacc aatgtatatt ttttaatttt tttaagtaga
     3601 taaatttgga aatctgaaat aaaccaacca ggcattatgt atcactatgt tatagagggt
     3661 tctacttaat ttagtgaact attggctttt gaccactatt tgttaatggc ttttatcaaa
     3721 ttatccaata gtctttctag acctgttgat aaattagtat aaatattttt aaccaataca
     3781 ctgtatataa ttatcctcct gtttaactgt aatttcatct acacatcaga tttacatctt
     3841 gcataatcct tacccatatc attttagtga tttgtagtat gaagaatgaa tgataatcac
     3901 atattaaatt aagtgactaa cataaattaa caatcttagt ccaacgtttg aatatactca
     3961 tttaattcta gaagtaaaaa ataatatttt ataaagaaag gaaagcaaca attaacgttc
     4021 tgataagtct attatatttg atcagtgcca aatagtagac agaagattca acgaacactt
     4081 taaagcgtaa tagagtataa tgaatacgtt ctacaattaa tttgtgggct aaaagattaa
     4141 tacaatccag ttttttgata ttgcccacga ggatacaaca actgagggca atggcatcaa
     4201 aaccaatatt taaaactcca atatttttac catttagttc aatcggtagt agagattttt
     4261 agattactct tattctttga cttatataat aaaatatttc taaaagattt tataaaaata
     4321 ataatagatt ataaaactac aatcatttta ttttattaag attaaaatat ttacctcttt
     4381 tatatttaaa attaatgtct tgtattattc ttccaaaatc tgaaaaatca tctcaattgg
     4441 caacgaaata taaattcaag gaatggatga ttgcaaggta ccttactatt gttccagaga
     4501 cagaaaaatt tcttgactat attaataata gagtatcgaa tcaatatatt cggataaata
     4561 cattaaaaat tgatattgat actataaaaa aaaaattatc agaaaaagat attaaattgg
     4621 agaaaaccct cctatccgaa gtttttctta ttaaggaatc aaagtaccct ataggagcta
     4681 ctatggagta tttatctgga tattattaca ttcaagacct tagttcatgt ctagctgtag
     4741 aatccttaga tattcaagat gatgatatgg ttttagatat ggcagcatca ccaggaggca
     4801 aaacctcctt catggctcaa aaaatgaata atagggggac aattcttgct cttgaatcag
     4861 aaagaaaaag gttaagaaaa atgatgttta atctatctag atgtggtgtt atcaacactg
     4921 ttctatggaa tctaaaagga gaaaaaataa taaattatga tttaaaattt gataaagtat
     4981 tacttgatgc tccttgttct tgtgatggaa taatgcagaa agatccgata agaaaaacta
     5041 cctattcgaa gaaaacaatc caatattgta gtctcatgca atctcaattg attgaatcag
     5101 ctctcaaggt agtaaagcct aacggattac tggtctattc tacatgttca ttagcgccag
     5161 aagaaaacga gttcataata aattctatga tagacaaatt tgatattaca atagagtcta
     5221 ttaattatgg tgttgattct ttattacaat ttggaggtaa aattatggat aaatccatcc
     5281 agtttacaaa aaggttctac cctcacattc acaatacagc cggctttttt attgcaaaga
     5341 ttagattaaa caatattgcg aaatgatatg ttaagagagt taacaaggca agaaaagact
     5401 ataatactta gagaattcaa cagatgggga atttttaatt atcttcatga taaagaattt
     5461 gcagtagaag cgtgtgaaaa caatcttaaa tgcctattta ttattcctgt agttttaaaa
     5521 agtttgattt tttatcctta tacttgttat ggaggattac caataggtaa ccttcaaaaa
     5581 aaaaaattta taccttctat agccttcttt gatattgtag ctaaaaatag taatagtttt
     5641 ttatatgtta ttgtcgatag taacggagaa aaattaattc tttatggtag agatattttt
     5701 ggcaattcaa ttctttatgc atcaccaaat attgaagaaa attcaatcct tttgatatta
     5761 aataaatcaa aggaattgtt aggaattggt agatctagat ttcatataac taaaatcaaa
     5821 gagaatggaa aagtaacaat aaatacttta atggacattg gcacttattt aagatcggaa
     5881 aataatcaaa aataaaaact attaattgtt tattttacct cacacattaa caaattaatt
     5941 aaaaattctc attataacta ctaagtaata ctaattaatt ttgttttctg taatgaaatt
     6001 gaattgtttt agacatttca tgctattctt ttttttaata tcatatttat taagagaatt
     6061 caatatattt actccgtgct acttaaaatc aaataaatta aggttatata aaaacaagaa
     6121 aatatttctt ttatgttaac atattaaagt attctattaa aaagtcacaa ttaatagtaa
     6181 atttatgaaa ttaaattgtt attcggattt tatcatagat gaatatattc ctccaattcc
     6241 ataagcaaca gatattaaga ttctagctaa aaaatcacca gaagatttgt caattatgtt
     6301 ttcattatat aataaaataa ttattccaat tgagattata gaaattaatc ctacaataaa
     6361 ttgatccaaa tttttaagat tattctttac cataaatatg aagaatgtag atagatttcc
     6421 taaaccaatt cctgccagaa ttagatattc atgaatagaa ggaattaatg ctcctaaacc
     6481 gaaaggagct gcccagcaca gtccatttat caatttaaaa gatggtgacc aggcagtact
     6541 ttttcttatt ttatttcgta tgatgtgaag agagagtatt attttacgtc taaatataat
     6601 aaaataaata aaaaaacaac ctatccatat tataatatcc attaacaaaa aatctaattt
     6661 taagtgtata atataactta atatagtagc tgaaaaaaca gataaagaaa taaagataaa
     6721 taaaatacca ttatcagatt ttccattttt ctgaatattc atagaaacct tgctaaacta
     6781 tctacctata aaagtatatt ttcttggata tcaaattact aattatatta aagaccttaa
     6841 aaaaataatt acgaacattt ggttaagtct tttgataaaa taaaaagatt tttcttattg
     6901 ttgttatcat attaattgtt gtcacctttt ttcaatattt ttaaaaagaa atctgaaaaa
     6961 gagagaataa agccaaaata tcccaataca atcgaccaga taagaacaag agaaactgaa
     7021 gagagaagaa caatttcaat agttgaagca aaggatataa ttaaaaaaat tgatcttagc
     7081 cagactaaga tcctgacagg gagaattaat aaaataagac atttaacaca aaaacctatt
     7141 ggaaacatta aagccttaat ttcagattta gaatctacga agattgatgt tcacgaaagt
     7201 agctttgaat cagtagtagt gaattcaaaa aaaatggtaa tcaattccat tagaaaggaa
     7261 ctttcctcaa atattccttc ccctgccact cttaatgatg ttatcaaatt gcatgaaaga
     7321 ctagattcaa tggtgaatag attttcagaa gttagcaagt cccataaaaa agtgttcaat
     7381 ttttttataa gcaaatatgc tgataaactt aaaacagaat ttgaaaatat atcttcatta
     7441 tcaatgcaat gcaagtatga aatagataat tttgaaaaag aaaggcaacc acttcaaaat
     7501 tgttttgaca atatcgatgc gctaatacag aaaattgaat tgattaaatt agaggaagaa
     7561 aaattcaaaa caaaacaatt tcaattagat gaaatgaaaa ttaaaattaa aaaattgaat
     7621 attgaaataa agaatttgac tgattcaaaa aaatatttag aatcaaaaga tgtagtaata
     7681 aaaatacaat ctctcgacct cgaaaaggat aatcttcata aagagttaat taatgctttt
     7741 agcaaggtga gtcgagcgat gaacaaatat tcttatggat taaataaatt aattgttcaa
     7801 agaattgaaa taatgacaga acaaccatgg aagatatttg acgatttaat tacttatttg
     7861 aatttaataa aagaagttaa aaatgcgatt agtaaaggta aaattatagt aaaagagtca
     7921 gagaaaatag agtcttattt agataatata ataaatgcat tacccaatta tcaggaaaag
     7981 gaagaaaaat taaatagtga aattaaaaaa ttgaatagta ataaaaatat gcacgttatt
     8041 tctaaattaa atgaattaaa aagaaaaaac gcaagttata atcaggaact tcagaacata
     8101 gaattgtatc taaaagattc tgaaagcgaa gtagcaaaaa ttaaagatga atatgaagat
     8161 ttagtagtct ctatcgaaac ttatattaat caaataacta aaagtaaata tcatttatca
     8221 atatctaatt aactgaaaaa aacagatgtt tatttaatga atttcttata aacttgtttc
     8281 aataattgct tgatatacaa ctaatgaatg tttatcacta tttattaaaa ggcttctacc
     8341 ttgatctatt cttattagag taagtaatat ttgatattca atttttaatt agattttaca
     8401 tttttaaaat ttttaacatc ctcaaataag aaactaattt tatgtttcaa tttttcccct
     8461 tatataacaa aaagaattta ttcattcaag gtgataatat tacaaatatt ttatcaatat
     8521 aagctggaat aatatttcag cgattacctt tgattagaca acgttattct aaaaatagaa
     8581 aatactataa gtctatataa taaataatta atgattaatg aaaatattga ataatttttc
     8641 aaaacctgta aaaataggcg tgacaatttt gcttatcggt gtagctgtaa tggcttacgg
     8701 tacttattta aatagtgata ccatttcata ttacgcttta ctaattatta ttactggtct
     8761 tttcatctat ctagcatata cttttaactt tgtaagcaaa tccaaggttg atcatactac
     8821 tacagaaaaa gagaaaggaa aaaaaaggaa aaaatgatat taccgctaaa gctacaaaga
     8881 gaatccaaaa ctttctgtag aaatctattt tttggatccg ttattttatt tatatatttg
     8941 cttacaactt gattaaatta taattagtat gatgcaaatg aagaatagtc gagctagtat
     9001 actgtttttg atcctatatt actttctctt cgtatacaat atttataacc ttaccaatga
     9061 gaaaatctaa tttagtaact gccacgtttg agatcaactt ttttttaaaa aaggataaat
     9121 attctgtgtc aagatagaaa caaataaacc gattaaattt ggcatcatta aaataaaatg
     9181 atagaaagtt aaagataaaa tgcaaggttt ttaaatatat gaactttatt agtgaagaag
     9241 aagactattt agtaaacatt gtactcagtt aattcgataa ttatgtaggt attataggaa
     9301 aatatgtata ctctaaaaat attttaaata tctagtagga tggttaataa caaggtaata
     9361 aataataatc tctcgtgaat aacttggggc ttatatttta cttaaatatc aaaaagatca
     9421 atattgaatg gataaggtag atatactatg tataaatttt acagtacaaa gtttcttatt
     9481 attatattat aatattatct ataatataaa tagagaatat acaagtgaac tagataaata
     9541 ttaaaaacaa aatataatca tatgcaacat tgtatagtga tagttttatg tagaaaagct
     9601 cgattttatc tttctatatc tttgacaaat gggaatttta ttaaagaaag gaaagaatct
     9661 actagaaggt acattgttag taatcatacc taaaataagt gatattttaa gatgagagta
     9721 tacattgctg caacaataat tggtatcgtt ctcctcatgg caataataac atttcacgcc
     9781 gatgaagctt tcgcaaaaaa aggtgattct tcttcaaaga aaaagattga attgtgttgt
     9841 gcatggggta aagctttaga cgacgggata ttaacatttt ctattaagaa tggtggatca
     9901 gacttggcga aaattgtgaa gctagcgata aatgattggg aaaaagccct cggtggagtc
     9961 gacttcaaat atgtaaaaga tgacaccgat gccgatatag aaattgactt taaaaagggt
    10021 aaaggaaaaa aagttggaaa aactgtgaca ttttttgatc atttgggact tatcgcccag
    10081 gtggatatat cgatctctaa aaaatcatat ggttttacat tagataaaca gactttagaa
    10141 catgttgcaa aacatgaaat gggacatgca cttggcttag gacatgcaac ttttcaaggt
    10201 actctcatgt ctcccaatgt agatgaaatc ataacaaaaa tatctgcttg tgaattggga
    10261 tcagtcaagt atgctaatag ttggaaattt aatggtggta agtcccccac ttctttagat
    10321 aaagataatt ttaaatgcaa gaaaaagtga ccctttgaaa agatattttt attaattatc
    10381 cagccttttt ctatatttca tattttaatc attatttgga agagctactt tctgtacagg
    10441 taaattattc ttcaaattat gtcataaatt aaacaaaact tataagatta atcatatatt
    10501 ctcaattatg gaaaataatt cactaactaa ggatattatg aatttggata agaaagtccg
    10561 ttttgcagct gtctgtgatg attccggtga gatcaaatac ggtgggcaaa gagaaggaat
    10621 tccgaattta ttatctccgg aagaaactaa aaggtccaat atccaggctt tagcaagatg
    10681 gtctttgcgt aattctcttg cctcaaaaat tggaaaagga caatatgcta tggctgtata
    10741 tgaaaaaatt aaacgtatca ctattcctct cgaaaatgat catcttcttt tagtaaccac
    10801 cgaaatagat gctgaccatg aaaaaataat tagtgaagta atgtcattac ttaaaaaata
    10861 gatttataat tatctttatt tttcttgtat caattcataa taaaattcac tcgatttaag
    10921 cctcatagat cattcgcctg tttctgttgc ctctctcctc gaatgtgatg cacctacccc
    10981 tcctaaatta ccagtaaaat caccagtaat ctttatgaca tcttctggct cagctaattc
    11041 attgagtatc tccagtgtaa gtctctgatt ggttacataa tgagtaccat tatcatatat
    11101 agctggtgcc attcctgttt ttctgattat aaattctctt tttgcttctt catctgttcc
    11161 atcctctgta aacatttcaa ccatgtaaaa gggtgcacta tcaggatctt tctgttgtct
    11221 tgataataaa tcattaacta ag
//
LOCUS       DASVEV010000160         8303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_834644, whole genome shotgun sequence.
ACCESSION   DASVEV010000160 DASVEV010000000
VERSION     DASVEV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834644"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..341
                     /locus_tag="VF222_07375"
     CDS             24..341
                     /locus_tag="VF222_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142283.1"
                     /translation="MFLIVPTVATAIFLGTVLTLSTFTISALAQAESMNQSMNNASAS
                     ANQAGESTGQSANQTGEAVQGNASEAGSNITEGAKNIGENISEGAKGLAETIGKGLQD
                     LAK"
     gene            1585..1812
                     /locus_tag="VF222_07380"
     CDS             1585..1812
                     /locus_tag="VF222_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142284.1"
                     /translation="MPLTVVATVLGVSTFIFTGYAQSEETNQTMQNAGQSANQTGGEL
                     QKNASDVGSKITEGGKDIVGKIGEGLENLGK"
     gene            2340..2639
                     /locus_tag="VF222_07385"
     CDS             2340..2639
                     /locus_tag="VF222_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX7142285.1"
                     /translation="MSNDTTDFNSEDIIKKEARGLGDDTDLNSEDIIKKEARGLGDDT
                     DFGEVQEILGEYIITKKGTVDKERYYIPKSLVERFDGETVYFKVTKEEAKQYKRD"
     gene            complement(2794..3285)
                     /locus_tag="VF222_07390"
     CDS             complement(2794..3285)
                     /locus_tag="VF222_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142286.1"
                     /translation="MKITEDIIAQYIISNNYEQPKIEVGNYYLKWNEKSYGIARKEIF
                     ELKGFLAKVIPYFDVIYQFINSIERPRSKEKFSLYNKLSPDIHFSHRNQNHLVSYDLY
                     MLPDGATFINRTEKIEIISKAYSNLATRRHVPKGNYSDLELFLKSKLGQLLFNRQIIV
                     RGK"
     gene            3741..4019
                     /locus_tag="VF222_07395"
     CDS             3741..4019
                     /locus_tag="VF222_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142287.1"
                     /translation="MSSNFSSTQKISQSATEHNKLTKVTSGYYEIDEFKDSDTHTSYF
                     CYNCEYFIKPNHCMIVTDEGQDINGKVSGTILPYAGCNLWTPNEKEIK"
     gene            4025..4357
                     /locus_tag="VF222_07400"
     CDS             4025..4357
                     /locus_tag="VF222_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142288.1"
                     /translation="MSDKNIFEKAGDIVGNVTKFKKQSKLTAGYVERDEFKDSNLKKG
                     PFCYNCIYWIDMTGGKCMIVDDKGPEGFGNVSNVIAAHGCCNEYEPNFDTILEKKEVV
                     IDNNKTST"
     gene            5662..6189
                     /locus_tag="VF222_07405"
     CDS             5662..6189
                     /locus_tag="VF222_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142289.1"
                     /translation="MSFQEDKKEIVDVKEKQQQKDHLSSIESNIGDYKSNVNFPSTFP
                     QEKNIAPPRNFDDLVNYPQTIKAMKEIKFNFLEEHKNIINTYYSIYSNRLQEILDCNL
                     NNLMSIEKYSKTYGKINRNLLDNQTNTIRIIDDIVVKNMDTFIKSIDLVHKFYFDVIQ
                     SYYNYIKKLKNPSYK"
     gene            6655..6846
                     /locus_tag="VF222_07410"
     CDS             6655..6846
                     /locus_tag="VF222_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142290.1"
                     /translation="MNKNIVSYTFLIALVVVITFTSLLSPFEEVVGQVDPSQEQIEPI
                     PPSNNTNQTAPIVTLQALY"
     gene            7205..7336
                     /locus_tag="VF222_07415"
     CDS             7205..7336
                     /locus_tag="VF222_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142291.1"
                     /translation="MCSVKADTSLPKDASASLTGSTRSRSPSTIIIDFAKAIFDNLF"
     gene            7905..>8303
                     /gene="nuoB"
                     /locus_tag="VF222_07420"
     CDS             7905..>8303
                     /gene="nuoB"
                     /locus_tag="VF222_07420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7142292.1"
                     /translation="MIKDLINPANFNLMVSKVSDVLVGALAEPLGYAINWGRIWSLWP
                     VHLETACCSVEFGATSSPRYDCERFGIIEAFGSLRQCDLIVVQGTVTRKMAPRLRMVY
                     DQMPDPKYVIAMGACSITGGLYIDSYNVLPG"
BASE COUNT         3060 a         1093 c         1236 g         2914 t
ORIGIN      
        1 attaacaaat gatatacaat aaattgtttc taatagtacc gacagtagca acagccatct
       61 tccttggaac agtgctcact ttatcgacat tcactatatc agcattagca caagccgaaa
      121 gtatgaatca atcaatgaat aatgctagtg cctcagcaaa tcaagcagga gagagtacag
      181 gtcaatctgc caatcaaaca ggtgaagcag ttcaaggaaa tgctagtgag gctggttcca
      241 acattactga aggagcaaag aacataggag aaaatattag tgaaggtgca aagggtcttg
      301 ctgaaactat tggtaaagga ttacaagatt tagccaaata atcttctttt cttttttaag
      361 accattcata attctgaatt gatatttctc ctgttatcta atcttccatc caaggtaatt
      421 cgtttatcag attaattttc aaaacattta tctctaaaac tgattcaata actatgtaag
      481 aattaatttt aaaatcaagg taataggatc tgacttaatg cgggatttca ctttacaggt
      541 acataatctt ctggagttta tttaatttta tttatagtac caacaagaag atgctaaaat
      601 gtagtacttt gtacattatt cttaagttta aaaagaagta ctatgaatgt ataatgaatt
      661 aaaataacaa ttaacatcat aataaaaaat agctatcaac catacaccat aaatatatca
      721 aaacttgaaa gttaatttgg gaataaattc aaagttcatt ccataaaatt cgaataagaa
      781 atgtcaatgg taattcaata gttgcaatga ctatcaaggt tctaattgtt tcattcattt
      841 ggcaatcaac gaagaattat aggtttctct caagctagtt attctctgat gtgaaattca
      901 acgttggatt aaatcatcta aactgttaaa aactactcaa ctgaaatata tgtaatttaa
      961 gaatggagat aagtgactaa aactatctaa cacacgttag tttagactaa aatctagatt
     1021 attttctgtt agattctctt caatctttta gatattactc ttagaattct taaatattgg
     1081 catattttat tcaaatatga ctagtatttt catcttaatt tagtattata aaaatgatgg
     1141 ttgaaatatt tgaaaattta aattatatat ttgctttaag taattttata taaactcata
     1201 tattagaaag ttgaagtttt aacggaatgt taaatatgtc gcatacttga tcatttactg
     1261 tgttgttata aaatattttt atttaatccc taagaatagc ctatgaattt gatttccagt
     1321 attctaaggt tttatatgtt aagcccaaga taattgcctt gcagtgaatt gttttttttc
     1381 actccacaca atgctagtaa ttttgaatat attttgcacc agggcaatat ttgaaaaacc
     1441 aataatgcat aatggagatg aataatttag tagcaacaat gaaacaatat tccacacttt
     1501 tttatagcaa tttgttacca aaaattttta tagtcaaaaa catataaaaa atttagtttg
     1561 aaatatacta aattatttgt aatagtgcca ctaacagttg tcgcaactgt gcttggggta
     1621 tcgacattca tctttacagg atatgcacaa tccgaagaaa cgaaccaaac catgcaaaat
     1681 gcaggtcaat ctgccaatca gactggtgga gagcttcaaa aaaatgctag tgatgttggt
     1741 tccaaaatca ctgaaggcgg aaaggatatc gttggaaaga ttggcgaagg actagagaat
     1801 ttaggaaagt aaaacaactt ttttctaatt tttttattat atttttagag ataataattc
     1861 ccatgaagat agaatacaga ttaaatatga tttacctttt ttagtggaaa gactggaatt
     1921 taaaagattt tcatatcaag tgaggaactt attgaatatt gctatgttta caagtgcatt
     1981 gtggtagcaa tttggtgaaa caattcgtga gaaacatatt gatattcata ttaaatactt
     2041 ttacgaaaat aagatttaaa agtcaatttc aaacattagt cttattatta agtttaaacc
     2101 ttattttagc accgctttcg atatcaaacc aaaaaggccg tgaggatata gaaaaatagg
     2161 aaatccataa agggcataat aatcatattt gtattgctat tcaattgttg agactatcta
     2221 agtttttatt aataatttta ttttaaaact tttaattttc ggttcacacc tgttatatag
     2281 taatactcta gattttaaaa taacacacaa gttaaatatg atgtgatact agcgattata
     2341 tgtcaaacga tactactgat tttaattctg aagacattat caaaaaagaa gctagaggat
     2401 taggagatga tactgatttg aattctgaag acattatcaa aaaagaagct agaggattag
     2461 gagatgatac tgattttggg gaagttcaag aaatattggg agaatatata atcactaaaa
     2521 aaggtactgt agataaagag agatattata ttcccaagag tttagtcgaa cgatttgatg
     2581 gtgaaacggt ttattttaaa gtgacaaaag aagaagcgaa acaatataaa agagactaac
     2641 tatacttatt ttctttattt tattataaat ataaaagtat taacaacaac ttctacctat
     2701 taaatagata atttttaaaa atccttattt acaacaacaa aattttttta cattatatgt
     2761 ataatatttg ttattgtaaa atttagttta ttactacttt cctcttacaa taatttgtct
     2821 attaaacaac aattgcccta atttcgactt caagaaaagc tccaagtcac tataattacc
     2881 tttgggcaca tgacgtctag tggcgagatt actgtaagct ttacttataa tttcaatttt
     2941 ttctgttcta tttatgaagg ttgccccatc tggcaacata tacaaatcgt agctaactaa
     3001 atgattttga ttacgatgcg aaaaatgtat atccggagat aatttattat aaagggaaaa
     3061 tttttcttta cttcttggtc tctctatgct gtttataaat tgataaatta catcaaaata
     3121 tggaataacc tttgctaaaa atccttttag ttcaaaaatt tcttttcggg ctatcccgta
     3181 tgatttttca ttccatttca aatagtaatt gccaacttcg atttttggtt gctcataatt
     3241 attagatata atatattgtg ctattatatc ttcagtgatc ttcaattagt cattaaatgt
     3301 tttttcaggt taataaattc tctttgccaa attcataatc aaatgtctct tgctgattgt
     3361 aaatcaaata tgactcaata atcatcttta tgaacagaag aaaaatgggt ttttgactac
     3421 attacttggt aattaaacta atttttgaaa ttcccgcgat tgtcactgtg cataatttta
     3481 tcgaaaaaat actttatcaa catatagtga tggtcggtta gatgataaat ataaaagaaa
     3541 aatacaaatt gctattatta caataactat aaaaattgaa tagtattatg attattattg
     3601 tcatttaata atagatcata ttaaggccat aaaaagtggt ataattacct attgatttgc
     3661 attttagagt aaatagatat gtctctagtg aaaaaacaag gcataattga tataaatgat
     3721 atagtaccta gggttattat atgtcttcaa atttttcatc aacacaaaaa atttcgcaaa
     3781 gcgcgactga acataacaaa ttaacaaaag taacatccgg ttattatgag attgatgagt
     3841 ttaaggattc tgatactcat acaagctatt tctgctataa ctgtgaatat tttataaaac
     3901 caaaccattg tatgattgtc acagatgagg gtcaagatat aaacggtaag gtatctggta
     3961 ctatacttcc atatgctgga tgtaatttat ggacaccaaa tgagaaggag ataaaataat
     4021 agatatgtct gataaaaata tatttgaaaa agcaggagat attgtaggaa atgtaactaa
     4081 atttaagaaa caaagtaaat tgacagcagg ttatgttgag cgtgatgagt ttaaggattc
     4141 taaccttaag aaaggtcctt tctgttacaa ttgtatttat tggatagaca tgactggagg
     4201 aaaatgtatg atagtagatg acaaaggacc agaaggattt ggtaatgtgt ctaatgtaat
     4261 tgctgcacat ggatgttgta atgaatatga gccaaatttt gatacaattc ttgagaaaaa
     4321 agaagtagtt attgataata acaaaacttc tacctaattg ttacgatagt aagagggagg
     4381 acgatctctt cacaataaca tattaaattt ttttaaaatt ccctttatat cttctacaat
     4441 taattattga ttatttatca atcatttttt tgatatttat ataatagtat taatttatat
     4501 caatatttta ttaccatgcc caaaaacaaa ggtatagaaa accttaagga aataaaaatg
     4561 aatatttttg aagagatgta tgagtataaa tcatcgtttt attttctata aatttagaca
     4621 ataaacctat tgtaaacgcc tgtgaggtaa aatgagatat accttaatta tctattaact
     4681 agttctctct tcgaaactaa ataaagggtg attataattc tagtgctatt tgtttttatt
     4741 taattcatga taaaaggagc agctttaatg tatttgagac cctcctcttc tgtatgataa
     4801 ttgtgataga cacggtaatg ctgctgataa agcagtagaa attgctaaat tggtaaagta
     4861 taaaattata attttagatg gtgttcctta gatagcatgt ctcggttcat aaaagtagtt
     4921 catttaatta aaatagtaaa ataatcattt ttcaatatag attgtacaat ttgattaaca
     4981 aatgtaattt gacatgaacg aaaaactata agacaaaaaa ggaaatataa taatatgatt
     5041 gtattatgta actgggtttc aaaatttttg aaagggttag gaagtgtttc tcgcaatctc
     5101 tgaaaaattt catctcttat ttttatggtc tctaaacttc aaagttcact ttcacatcta
     5161 aaaaaccaat gcattattat aatgcagaga aaaaaaataa ttaactttat taagatacag
     5221 gttcagtttt taacaattac catgacttat ttctattgaa gtcacttaca cgcatacata
     5281 tctatattat cataaaaaaa ggctattgat ataatcaaca cttactgatg tattctactt
     5341 gattaaattg atcagagaat ctatggcttt ttcaagaaag ttttgctaag tataatttac
     5401 agaatatctc taattcatca atagtattgc ctatctagta atataccaat ttcatttagc
     5461 ataaaatata ttttcaggtt tgatcttttg gaaaaaagaa ttcttcctcc ttttttgatt
     5521 atttggacat gaaacaacat taggttttag aagagttact tttaagaaac taaaattcca
     5581 tcaacttttc tcataaggtt ttgaagacta gttaataatt ccttttttaa tgtttttaag
     5641 taagaagata ccacaatgtc tatgtctttt caagaagata aaaaagaaat tgttgatgta
     5701 aaagaaaaac aacaacaaaa agatcattta tcatctattg aatctaacat aggagattat
     5761 aagagtaatg taaattttcc atcaactttt cctcaggaga aaaatattgc tccgccaagg
     5821 aattttgatg atttagttaa ttatccacaa acaattaaag ccatgaaaga aataaagttc
     5881 aacttcctgg aagaacataa gaatatcata aacacatact attcgatata ctctaatcgg
     5941 ttacaagaaa tattggattg taatttgaat aatttaatgt ctatagaaaa atattctaaa
     6001 acatatggta aaataaatcg aaacctcctt gataatcaaa ctaatactat taggataata
     6061 gatgatatag tagtgaagaa tatggacaca tttattaaat caatagatct tgttcataaa
     6121 ttttactttg acgtcattca aagttattat aattatataa aaaagctcaa aaatccatct
     6181 tacaaataat atttgtcact tattattatc ttttttctta acatttattt tttttaaaag
     6241 atttgtcata ctctattttc tggcatggct tcaattatta gatttattga atgtaactct
     6301 atctaatatc attatgtaaa gtatattcat aatgatctat tttgaatttt atgctgaatc
     6361 ctaaagtgag aagatataaa aatagtataa aattcagcaa attataagtt atcaaatatt
     6421 gtttgaaaat atcttattat attattaatg tgtatcttat actgatacta cttccaaata
     6481 taaagcaaat taaaaaatat ccgaatatag agcactgaac gatatctgat atcttagttt
     6541 ggtattacct cttaaagacg gtaagaccgt attagttatc tataaaattt caaactattc
     6601 aaaatagaga ggataaatcc tatatatgat aatgtgtatt taatattgat taatatgaat
     6661 aagaatatag tttcttacac atttttaata gctttagtag ttgttataac tttcacatcc
     6721 ttgttatcac catttgaaga agtagttggt caagtagatc catcccaaga gcagatagag
     6781 ccgatacctc cttccaacaa tactaatcaa actgctccaa ttgtaacctt acaagcattg
     6841 tactgaatga ctatttctag ggtttcagaa ttggttcaaa tttgtctact tgctaacatc
     6901 gacttaatgt acaatctcta acacggcttt agcgagagaa gatatacgtt agatcagagt
     6961 tattttaaac agttgttaac tttctccatt aatagtttta gtgggatagg tttttgaggt
     7021 agttcaaagt tgagcgagtt accttcaata tattcatgtg tattgataag aataattttt
     7081 attttaaagg tagattcttt gatccgtata cccaaattgc aaccattcaa ctgttccatg
     7141 tcaaagtaac ataccaacaa actatacttt ttttggatac ttttgaacat gagtgaatgc
     7201 cgttatgtgc tcggtaaaag cagacacatc attgccaaaa gatgcaagtg cctccctaac
     7261 gggctcgacg agatcacgct cgccgtctac tataataatt gattttgcta aagcaatatt
     7321 tgataacctg ttctgatgct atatgttgat gaatatacca gaaagtattt tctaatgttt
     7381 tttatggatc aatagaacaa agcatacaat atttgagtta gttttattaa agatacaata
     7441 aattatggaa atatacaaca ataacaatta attttctatt atcatcgata tattattatt
     7501 actttcttgt acactatagt gaaaaatgac ctgtaaagga atatgtcaac gtcataaagc
     7561 acttagacct agaaacggga aacgttactc attagggcaa aaacgttgtc aaacatgtga
     7621 tatttttatc gaatggcata atatctggtg tccttgttgt ggttatagat taagggtcaa
     7681 acccagaaac ggaaaattta agtcaatatt gtaaaacaat tataaattcg cgcagagaat
     7741 caacgatatt aacattatta gttattacat ttataatatt aagaaaaaca ataataataa
     7801 taataataat tataaggagt ttatagtgcc taaacaaaga aggaagtaat taatagatta
     7861 aatacctttg ttgtgtattt attaatagag gaactcgatg ttaattgatt aaagatctta
     7921 ttaatcccgc aaattttaat ttgatggtaa gtaaagttag tgacgtttta gtaggggctt
     7981 tagctgaacc attaggttat gcaattaatt ggggaagaat ctggtctctc tggccagttc
     8041 atcttgagac tgcatgttgt agcgtagaat ttggtgctac ttctagtcct cgttatgatt
     8101 gtgaaagatt tggaattatc gaagcttttg gttctctgag acaatgtgat ttaatagttg
     8161 ttcaaggtac tgtaactaga aaaatggctc ccagattgag aatggtatat gatcaaatgc
     8221 cagatcccaa atatgttatt gcaatgggag catgttcaat tactggaggc ttgtatattg
     8281 attcctataa tgttttgcct gga
//
LOCUS       DASVEV010000161         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_834888, whole genome shotgun sequence.
ACCESSION   DASVEV010000161 DASVEV010000000
VERSION     DASVEV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834888"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF222_07425"
     CDS             complement(<1..218)
                     /locus_tag="VF222_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142293.1"
                     /translation="MPLNFLLKTNDKTFHTFKYIILPSIFLSIIALVSTNQSLWITSP
                     IHHFYIELFGAMLAGILSVYYISRANNL"
     gene            complement(328..1410)
                     /locus_tag="VF222_07430"
     CDS             complement(328..1410)
                     /locus_tag="VF222_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142294.1"
                     /translation="MKLFSSKGVRFSGDSIKSCVGFIDLVDSTKNAITMDNLEHIRKY
                     YSKFINSTSAVVKSYTGKVIKNIGDCLLFYFPKTTDFKDMEVFKQTIDCGFKILDERY
                     IVNQELFKEHLPPFNYRITIDYGVLDLALVGDYSQIDLFGTTINLCSKINSSSLSIPN
                     EIIIGNNFYRILKSFPTIMNNSNFINNGEYRITESNKYSTYNIKRKNILELNDMEISN
                     KTSLSEKQQGSSIEDFNNKNDNKRVILVDDEQDILFTYKVFLETDYDVISFADPIFAL
                     NYIRNISNFNHLLIILDIRMKNLNGFQLHQQIKAIDPTIKILFVTALDILDEFSTIIP
                     GISKGQIMKKPVDKKLFTNTVKQLLN"
     gene            1733..2179
                     /locus_tag="VF222_07435"
                     /pseudo
     CDS             1733..2179
                     /locus_tag="VF222_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            2991..3740
                     /locus_tag="VF222_07440"
     CDS             2991..3740
                     /locus_tag="VF222_07440"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7142295.1"
                     /translation="MNKLEGKVAVITGGNSGIGLATAKRFVAEGAHVFITGRRQNELD
                     AAVSELGKNVTDVQGDVSNLVDLDRLYSIVKQQKGRIDILFANAGLGEFATLGQISET
                     HFDKTFNVNVKGLLFTVQKAISLFKDGGSIILNSSIAASKGVEGFSVYSASKAAVRSF
                     ARTWTVDLRHRQIRVNAISPGPIDTQAFSDLMQNEGQSRQLKKDLVSTVPLRRMGTPD
                     EVAKVATFLASDESSYVTGIELFVDGGAAQI"
     gene            complement(4298..4756)
                     /locus_tag="VF222_07445"
     CDS             complement(4298..4756)
                     /locus_tag="VF222_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142296.1"
                     /translation="MNRSRLKDYLLFNRNIIIGFVGAFLTSAGTSQVIAKITSPIINS
                     LISIAVETSIFLAIFGILFYLENKNKFVDEQGKRKESAKVKWVILKLVSTLSIAEIEY
                     NTVKPAIHFWLLSLDYQPFIASVIASFISIIGYLVVADSMAYFTRLFKKS"
     gene            4917..5078
                     /locus_tag="VF222_07450"
     CDS             4917..5078
                     /locus_tag="VF222_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142297.1"
                     /translation="MDISTNDVIKWKNVTTNISTYDDNTIVTDFDDKATDNDFIRQSV
                     YGTDESALS"
BASE COUNT         1846 a          826 c          820 g         1852 t
ORIGIN      
        1 cttaaattgt tagcacggga gatataatag actgagagta ttccagcaag catcgcacca
       61 aataattcaa tatagaaatg atgtatgggt gatgttatcc ataaagattg attagtagaa
      121 actagagcaa ttattgaaag aaaaattgaa ggtagtataa tatatttgaa tgtatgaaat
      181 gtcttgtcat tagtctttaa aagaaaattt aatggcaatg gattaatctg ctactccttt
      241 tagatttccg ttagatattt tatatgtaat ggatagaata gacatagaat tcctttttaa
      301 tatattattt taatactgaa tgtcccttta atttaataat tgttttacag tattggtaaa
      361 caacttttta tcaacaggtt ttttcataat ttgtcctttt gatatacctg ggattatagt
      421 tgaaaattca tctaatatat ccaaagctgt tacaaaaaga atcttaatgg taggatctat
      481 agcttttatt tgctgatgta attgaaatcc attcaaattt ttcattcgaa tatcaagtat
      541 tattaataaa tgattaaaat tggaaatgtt tcttatataa ttgagggcaa aaataggatc
      601 tgcaaatgaa attacatcat aatcagtctc taaaaaaacc ttgtaagtaa aaagtatatc
      661 ctgttcatca tcgactaaaa ttactctttt gttatcattt ttgttattaa agtcctcaat
      721 agatgaacct tgctgtttct cactaagaga ggttttgtta ctaatttcca tatcattgag
      781 ctctaaaata ttttttcttt tgatattata tgtagaatat ttattacttt ctgttattct
      841 atattcacca ttattgataa aattggaatt gttcataata gtaggaaaag attttaaaat
      901 tctataaaaa ttatttccaa taattatttc attaggaata gacaaagaag aagaatttat
      961 ctttgaacat aggtttatag ttgtaccaaa taaatctatt tgactataat caccaactaa
     1021 agctaaatct aatacaccat aatctatagt aatcctatag ttgaatgggg gtagatgttc
     1081 cttaaataat tcttgattaa caatatatcg ctcatctaat attttaaagc cacaatcaat
     1141 agtctgttta aagacttcca tgtccttaaa atcagtagtt ttgggaaagt agaataataa
     1201 acaatcaccg atatttttaa taacttttcc ggtataactt ttaactactg ccgatgttga
     1261 atttataaat ttagagtaat attttctaat atgttctaaa ttgtccattg taatggcatt
     1321 tttagttgaa tctaccaaat caataaagcc cacacaactc tttattgaat caccagaaaa
     1381 tcttactcct ttagaagaaa aaagtttcaa tcttaaaaac cttcataatt ctttaaaaca
     1441 ataagaatat ccgtaaaatg aatatcgact aaataagaat gaaaattctg ttccatttaa
     1501 ttaatcaagt cgtcatctta ttaaaagaag ttgatcaaat gagatcttca tttattttca
     1561 aatctatcta attttaaaac agtaatagca ataaatagaa ctgcaattgt cattgtctta
     1621 gtaataaaag atgattttag agagaatgaa aatttggaat catgtttaat gatattatta
     1681 ctataacaaa ttttttcggc gccggaaaaa tctttagttt tactttgaca aaattttcgg
     1741 cattaatgtc aaaggacttc tgttcactgt gcaaaaagcg cttccgcttt tacaggatcg
     1801 cggttcgatc attttgactg gtttcatagg tgcatacaaa ggaatgaagg cattaagtgt
     1861 ttaccgtgca accaaggtct ccatacgatc gttcgcgcac acctagactg agatctgaaa
     1921 cagcgcaaga tttgtgtcaa cgctctaaat cctgggccta tcgatacact tggtgttgat
     1981 ggcttggctc agagtggaga agagatcgag caattaaaga agagcctcat aaccgctatc
     2041 cccaatgggt agaatggata gcccttacga agtcgcaaaa gttgtttcat tgcttgcatc
     2101 ggatgaaagc agttttgtta ccggtatcga actgtctgtt gatagaggta tgacacaaat
     2161 ccaaattgtt atttgttaat gcctcttttt tctaaccttc tatttccatt caaaatttct
     2221 gatattgtag tttatgtttt tcaaagtgac atttctctga acaataatag tcattgcaca
     2281 tattacagaa aataagataa tcctgactct tcctgccaca attagcacaa caactagaca
     2341 tatccatata taataataga ttattatact atgtaaattt gtctataata ttgaaatatt
     2401 tattttaagt aaaatcattc attaatttaa aagtataatt agagcgactt tttaagctat
     2461 ttatataacg atttatcaaa aataaaattt tattataaat gaagatgtat attcaatttc
     2521 acattgatga gaaatctatt gggctataac ttttttttat aatataaaat tttgaaacat
     2581 gtttatattt ttaattgttg gtaaacataa ttaagtctat agatctattg tgccaaccac
     2641 tatctcatta attattatta ggaatggata tagtattaaa atttgtttag acatttactt
     2701 ccatttaatt taagatgttt aattattctt aacatattaa tataaagata ttctttgcat
     2761 atagaatgaa catatagatt atctttattt tgataacgat attggcaagt ataattatca
     2821 tattttaaaa tctatctatt gttttaattc agaataaatc atctattatc accttacatg
     2881 gtcattctcc ggttaatgta gaacttattc aaagattaac aagagccaaa ctagccgatt
     2941 aactcataaa aactcataag cttaaaacct actaaacaga agtaaaaatt atgaataaac
     3001 ttgaaggaaa agtcgcagtc attacaggcg gaaatagtgg tataggcctt gccacggcaa
     3061 agcggttcgt tgctgagggc gcacatgttt tcatcaccgg tcgccgtcaa aatgaacttg
     3121 atgcagctgt atcagagtta ggtaaaaacg tcaccgatgt tcagggtgat gtctcaaatc
     3181 ttgtagatct agatcgattg tactctatag taaagcaaca aaagggacgc atcgacattc
     3241 tgttcgccaa tgctggcctt ggggaattcg ctacattagg gcagatttct gaaacgcact
     3301 ttgacaaaac attcaatgtc aacgtcaaag ggcttctatt cactgtgcaa aaagcgattt
     3361 cgttatttaa ggatggtggt tcgatcattc taaattcatc aatcgctgca tccaaaggag
     3421 ttgagggatt tagtgtttac agcgcaagca aggccgccgt acgttcgttc gctcgaacat
     3481 ggacagtcga tctgagacac cgccaaatcc gtgtcaacgc catcagtcct ggtccgatcg
     3541 acacacaggc attcagtgac ttgatgcaga atgagggaca gagcagacaa ctcaaaaagg
     3601 acctcgtgag cactgtgccg ctgaggagga tgggcactcc tgatgaagtt gcaaaagtcg
     3661 ccacgttcct tgcgtcggat gagagcagtt atgtcaccgg tatcgaactg ttcgtcgatg
     3721 gaggtgcggc acaaatctag tgtctgaccc tctgcatttt tctatgctat tcataatcta
     3781 gtggtttcag taaaaggttt gtttaaaaat actgactaaa tatgttttct aaggttaaga
     3841 gtgttaaatt gtacagtcat tcaggcacta tattattgat ctcactataa tatgtaatat
     3901 aagtagtaaa gtaactcact cagtaaaaag ttttaaatca cttcttgcct tagttctttt
     3961 ttaatattct atcggaaaca ataggcgaag acttgaaatt ttagttattg aacccattta
     4021 gtcgaatgaa cgtagtagat acaaaccgag agtgaattaa ttcattattt agtattagca
     4081 acaaaaatat acgaaaagtc ttaagcaaga caatctatat tttaattatt cttagatata
     4141 ccaccttata caataagaca aagattttat cattatacat acgtaatctt taaaaatctg
     4201 aacaagaaat atagttgaat ttttttatat gaataaatct tcaaggcaaa agtttggaac
     4261 tttgtttatt taatattcag ttgcttgctc tgctatccta tgatttttta aataaacgag
     4321 tgaaatatgc catagagtcc gcaacaacaa ggtatccgat tatactgata aaacttgcaa
     4381 tgaccgaagc aatgaagggt tggtaatcca gactcaggag ccaaaagtga atagcaggtt
     4441 ttacagtatt gtattctatt tctgcgattg aaagtgtgga tactagtttt aagataaccc
     4501 acttcacttt tgcagattct tttctcttgc cttgttcgtc aacaaacttg tttttgttct
     4561 ccaaatagaa tagaataccg aatattgcta agaatatact cgtttcaaca gctattgaga
     4621 ttaaagaatt aataatcgga gacgtgattt ttgctatcac ttggctagtt ccagcgctag
     4681 taagaaatgc accgacaaag ccaataataa tatttctgtt aaataaaaga tagtctttta
     4741 atcgactcct attcaattaa ctcccctatc tgcttgaaaa aacatcttca ctcccatttt
     4801 acatccttgg caaaattcac tatttcttat agatcatttt ttctagagga cttaatatta
     4861 agttcacctt tctcatcaat agtcattcag gtaataatct caagctattt ggtacaatgg
     4921 atatatctac taatgatgtt ataaaatgga agaacgtaac gacaaatatt tctacctacg
     4981 acgataatac tatagttaca gattttgatg ataaagctac tgacaatgat tttataagac
     5041 aatcagttta tgggactgat gaatcagctt tgagctagaa tttgtatact ttaaagaata
     5101 gaataaacat ctataatact tttattaatc aaatttataa taatcatttt tctttttgtt
     5161 ctattttcta tctcaatcat tcttacacta ttatcttgga tacaatcact atcacaacct
     5221 aaagttaatt tttagataag gttttctagc aattaggttt agacaaatac ataatgatgt
     5281 tcgatattat taaaaataat aattttatat aatcctacta ctatagaatt tcaaagtata
     5341 tatt
//
LOCUS       DASVEV010000162         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1418396, whole genome shotgun sequence.
ACCESSION   DASVEV010000162 DASVEV010000000
VERSION     DASVEV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418396"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..843)
                     /locus_tag="VF222_07455"
     CDS             complement(154..843)
                     /locus_tag="VF222_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7142298.1"
                     /translation="MSTNNIDWNDVMKKEARGNDDEDFGEVQDIQGNYVLVQKGIINK
                     EKFYIPKDRAESYDGNILKFRFSKQDLNQYQREPPSIWDSDSTPETTTNERDTNEESI
                     PLTEERLDVSKKSQENQATITKKPVTETKTVEVPLTREEVSIERRPASGQTEAQSPIQ
                     SQEEIKIPLKREEAQVSKKPYVKEEAIVKKKAVTDKKEIREDVTSEELDTSKVDKESR
                     NLTIKISYLNN"
     gene            948..1931
                     /locus_tag="VF222_07460"
     CDS             948..1931
                     /locus_tag="VF222_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7142299.1"
                     /translation="MDIIDVNSFIFIFLSALQMNGTSVISPVSNTLNQLFSQIVESIP
                     KVIAAAIILLIGYIIGRVVGWAIKKVLEKMNFEKTIQKTTLGETVSRSGWSMTKIIST
                     AASWFIYLFFIVAAVNALQFTQLSEALSSIWLWIPNLIAFIIILVIGSIIADFVGNWI
                     QRELPKRGVPAGKAIGMGVKGILYAIIFVTAITQLQIGAGILNMVITALVWGIAAAIA
                     IGLGIALANALKDILPGVVHSTTNIHSTLKPGQKIKFERHSGTIKEVGAFQIILENDE
                     GQTVIIPTKSIIDKDIIVESGPAPETPEKKMQQMVEQAEKKEKEEEGDMTK"
     gene            complement(2330..>2618)
                     /locus_tag="VF222_07465"
     CDS             complement(2330..>2618)
                     /locus_tag="VF222_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7142300.1"
                     /translation="SKPKSSNEVFIGKKPLMTYVTATLVQLANEPTVFIKARGKSIPM
                     AVDVAQIIVKRMNTLGYKVASVKIGSETVKSQDGNTRNVSTIEVAVSRNSS"
BASE COUNT          852 a          393 c          397 g          976 t
ORIGIN      
        1 gcttcgtcgt gaaaatagac caaatatatc atccatgtcc atgttatatt ataaacaata
       61 aatcttataa tgctttttag cgtttattta tttctttatt atattattta agttggaatc
      121 atcattgtaa aatacaataa aaaaaatttt tatttagttg tttaaataag atatttttat
      181 agttaagttt cttgattctt tatctacttt tgaagtatcc aattcttcag atgtgacatc
      241 ttctcttatc tcttttttgt cagtaacagc cttctttttt actattgcct cttctttaac
      301 atatggtttt tttgatactt gtgcttcctc tctttttagt gggattttta tctcttcttg
      361 cgattgtatt gggctttgtg cttctgtttg gccacttgca ggtcttcttt caatacttac
      421 ttcttctctt gttagaggaa cttcaacggt tttagtttct gtaactggtt ttttagtaat
      481 tgttgcttga ttctcttgag actttttaga tacatccaat ctttcttctg ttaatggtat
      541 actttcttca ttagtatctc tttcattagt tgttgtttct ggagtactat cagagtccca
      601 tatagatggt ggttcacgct ggtattgatt taagtcttgc ttagaaaacc taaattttaa
      661 aatatttcca tcataacttt ctgctcgatc tttcggaatg taaaactttt ccttattaat
      721 tatccctttt tgaactaata catagttacc ttgaatatct tggacttcac caaaatcttc
      781 atcatcatta cctcttgctt cttttttcat tacatcattc caatcgatat tattagtact
      841 cataaatcat aatgacttta aaattattta agcatattag acaattttgc taaattagaa
      901 ttattaacgt agatattcat atttattatg ttgtacattt tataataatg gatataattg
      961 acgtaaattc atttattttt atatttcttt cagctttaca aatgaatggg acatcagtaa
     1021 ttagtcctgt ctcaaataca ctaaatcaac tattttctca aattgttgaa tctataccca
     1081 aagttatcgc tgctgcgata attcttctta taggatatat aattggtaga gtggtaggct
     1141 gggcaataaa gaaagtattg gagaaaatga actttgagaa aacaatacaa aaaacaacat
     1201 taggagaaac agtctctcgt tcaggatgga gtatgactaa aatcatatct actgcagcta
     1261 gctggtttat ctatttattc tttattgtgg ctgcggtcaa tgccctccaa tttacacaat
     1321 tatcagaagc actctctagt atttggttat ggataccaaa ccttatcgca ttcataataa
     1381 tattagtaat aggttccatt atcgctgatt ttgttggtaa ctggattcaa agagagcttc
     1441 ccaagagagg tgttcctgca ggtaaagcaa taggaatggg agttaagggt attttatacg
     1501 caataatttt tgttacagcg ataactcaat tgcaaatagg agctggaatc ctaaatatgg
     1561 taataaccgc tcttgtatgg ggaatagctg ctgctatcgc tataggatta ggaatagcat
     1621 tagcaaatgc tcttaaagat atacttcctg gagttgtaca tagtactacc aatatacatt
     1681 ctactctaaa accaggacaa aaaataaaat ttgaaagaca tagcggaact ataaaagagg
     1741 tgggagcatt ccagataatt ttagaaaatg atgaaggtca aactgtaata attccaacaa
     1801 agtcgattat tgataaagat atcattgtag aaagtggacc tgctccagaa actcctgaaa
     1861 agaagatgca gcaaatggtt gagcaagcag aaaagaaaga gaaagaagaa gagggagata
     1921 tgacaaaata ataaaggcag gtaactatac aaaacaatat ttattttact atcgtagtat
     1981 ctttgtcttt gatagagtac acattttaca atttattata atctacaatt gtaatttata
     2041 atttttaata taactaattt taaaatgatt gattaaaatt aatacagaca aacagattat
     2101 aaatttaaag aaacaaactc aaagaactgt ccttattact ggaactacaa gaggcatagg
     2161 taaataaata aactgcatta cttttattga aaaaggaatt aaatgtaata ttttttgttg
     2221 ttaatagttt aatatgatat tctcatctta acataataat gtaatttaag ttttataaaa
     2281 gatctatttt agtttagaaa tatccacgat agtttaaaaa taaaacaatt taagagctat
     2341 ttctagacac agctacttca attgttgaga cgttgcgagt atttccatct tgagatttga
     2401 cagtttcgga accaattttt accgaggcta ccttataacc taatgtattc attctcttta
     2461 cgataatctg tgcaacatct actgccatgg gtatactttt tcctcttgct tttataaaaa
     2521 cagttggttc gttagccagt tgaaccaatg tggctgtgac gtaagtcata agtggttttt
     2581 ttccaataaa tacctcattg ctagattttg gtttacta
//
LOCUS       DASVEV010000163         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_835649, whole genome shotgun sequence.
ACCESSION   DASVEV010000163 DASVEV010000000
VERSION     DASVEV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835649"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..353)
                     /locus_tag="VF222_07470"
     tRNA            complement(join(237..296,339..353))
                     /locus_tag="VF222_07470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Asp,seq:gtc)
     gene            616..831
                     /locus_tag="VF222_07475"
     CDS             616..831
                     /locus_tag="VF222_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142301.1"
                     /translation="MIYSNHLYLTSDIQAQIIVDESNESDERDESISIWEHEQPKVLK
                     VKFRNVNGHDNISKRKRKTSNKISRRE"
     gene            773..1720
                     /locus_tag="VF222_07480"
     CDS             773..1720
                     /locus_tag="VF222_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142302.1"
                     /translation="MIISAKEREKLVIKYLEENKSYKEIEDLLHISSRDISVIHKKAR
                     EKKEQEEKNEIITSVTSKALKLYKEEKSPLDVAITLGITAQQAQKLYIDYLSLTNSHQ
                     LAQIYQQLDEKLIPALVNLLYHIKKNEIMISEEEIVEAIRNINKIPRSRKEYNEISTE
                     IANLKRQRDTYINNKNFWKKQISNLTSNYDQLVAKSDFKRKEIKSLDKQIERKKELLD
                     DLKDSEYFEKVKQKIKDETNNILYSKKAFLKLAITTILETIKKDQEKNILIDNLLNYT
                     DNPPYPEEYLVYYQQKISEMADSLYDTISEIIVVNVTDP"
     gene            complement(2052..>2859)
                     /locus_tag="VF222_07485"
     CDS             complement(2052..>2859)
                     /locus_tag="VF222_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7142303.1"
                     /translation="IKDLLGTSEGASKISLGAGGDISRKIDIVAETAVLNTIKSNNIS
                     PVIIGEECGIVNLNKTSSSSSLSNNNNGFVIMDAVDGTTNAIRGIPFSCCSLAFASEF
                     KLSFVTDAVVLDLFTGDIYSASKQKGSFLNNKKINVRNEKDFSSITSLEDLKSIDVLI
                     GTNVSGVPLNILDEISRVISFSSHIRHFGANALELCYFARGFMDAYIDIRGKIRSTDM
                     AAAYLIVKEAGGKLYSPNGQDLDSELGLKNKISFYAVSNRRLFDLIKSSV"
BASE COUNT         1158 a          407 c          387 g          907 t
ORIGIN      
        1 gacttaaatt aaaaatactt atttatttac taaatcaaaa gcaatctaaa aaaatatagc
       61 tagagaattt gacagggtca atccggagtt taaatgtatt aaggaacata ttgtatcata
      121 atatctcaaa aatagaaata agatttacac aatgatataa caattcatta gcgaaaatga
      181 atagtctgga tctttccaag gtaaaagcca tataaagcct caatattgat tgtaagcgcc
      241 cgggccggga ttcgaacccg ggtcgcagga gtgacagtcc cacatactga accggaatat
      301 acgatgcaaa attaattaat aattcttata gagcatctct atactacccg ggcgcattaa
      361 agttgtttta taaaagcagt taaaatatct ttctttttag tatctatctg aactgataaa
      421 tgactatata aataatatat aataataaat attgcagatt atatttgcta atacttataa
      481 atttgataat aaactaaaat aatttttatt aattatgggt gaattgagtt actttacttt
      541 ttgagacaat gttttatcta tctatttact ttacgataat ccattaaata ttcttcttaa
      601 atgatatttt attttttgat atattctaat catttgtatc ttacctcaga tattcaagca
      661 caaataattg tagatgaaag caatgaaagc gatgaaaggg atgaaagcat atccatttgg
      721 gagcatgagc aacctaaagt tttaaaagta aaatttcgaa atgttaatgg acatgataat
      781 atcagcaaaa gaaagagaaa aactagtaat aaaatatcta gaagagaata aatcctataa
      841 agaaattgaa gatttgcttc atatttcttc tagagatata agtgttatac ataaaaaagc
      901 aagagagaaa aaagagcaag aagaaaaaaa tgaaataata acatcagtta catcaaaagc
      961 attaaaatta tataaagagg aaaaaagtcc cctagatgtt gccataacat taggtattac
     1021 cgcacaacaa gcacaaaaac tttacattga ttatttatct ctaacaaact ctcatcagtt
     1081 agcacagata tatcaacaac ttgatgagaa attaattcca gccttggtta acttattgta
     1141 tcatataaaa aagaatgaaa taatgataag tgaagaagaa atagttgaag caatcagaaa
     1201 tattaataaa attcctagat cgcgcaaaga atataatgaa atttccacag agattgccaa
     1261 tctcaaaagg caaagagata cctatattaa taacaaaaat ttctggaaaa aacagatttc
     1321 caatttaacc agtaattatg atcaacttgt cgctaaaagt gactttaaac gaaaagaaat
     1381 aaaatcattg gataaacaaa tcgagaggaa aaaagaatta ttagatgatt taaaagacag
     1441 tgaatatttt gaaaaggtta aacaaaaaat taaagatgaa actaacaata ttttatatag
     1501 caagaaagct tttttaaagt tagctatcac gactatttta gaaaccataa aaaaagatca
     1561 agagaaaaat attctaattg acaacttatt aaattatact gacaatccac catatccaga
     1621 agagtatctt gtttattatc agcaaaaaat ttcagaaatg gccgacagct tatatgatac
     1681 tatttcagaa attattgtag ttaatgtcac agacccctaa tatatattat tataaataag
     1741 tatggataat ataaattata atactattgg cttggacaat ttataactaa aacattattc
     1801 aaattaaacc tataacagta gattattaat aacatatttt ttaaagaata tatgatcatt
     1861 ataaaagtac aaattacaga tgaaaatatt acttaatagt tgaatgttaa agtggatgaa
     1921 aattgtttta atattaattt aaaaatttaa atttaaaaac atttattagt aaaaaaggtc
     1981 gaaatagagg aagttcatat taacaatcga tcaaaagaat attagattct atttaaataa
     2041 attcctcatt ctcatacaga actttttatc aaatcaaaaa gtcttctgtt actcactgca
     2101 taaaacgata ttttgttttt taatcctaac tctgagtcta aatcttgacc attaggagaa
     2161 tacaatttac caccagcttc ttttactatt aaataagccg ctgccatgtc tgtagatcgg
     2221 atctttcctc tgatatctat gtaagcatcc ataaatcctc gagcaaaata acacagttca
     2281 agagcatttg ctccaaaatg ccgtatatga gaagaaaatg aaataactct tgaaatttca
     2341 tctaaaatat ttagaggtac tccagataca tttgtaccta ttaatacatc aattgattta
     2401 agatcttcaa gtgaagtaat cgagctgaaa tctttttcat ttctaacatt tattttttta
     2461 ttatttaaaa aagagccttt ctgctttgaa gctgaataaa tatctcccgt gaacaaatct
     2521 agtactactg catcagtaac aaagcttagt ttaaattcac tagcgaaagc tagagaacaa
     2581 caggagaaag ggataccacg aattgcatta gtagtgccat ctactgcatc cattatcaca
     2641 aaaccgttgt tattgttaga caaagaggaa gaagaggacg ttttatttag attaactatt
     2701 ccacattctt ctccaattat tactggagat atattattgg atttaattgt atttagcact
     2761 gcagtttctg cgacgatatc aatctttcta gaaatatcgc ctccggcacc aagacttatt
     2821 ttactagcgc cttcacttgt tcctaacaaa tccttaatt
//
LOCUS       DASVEV010000164         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1615355, whole genome shotgun sequence.
ACCESSION   DASVEV010000164 DASVEV010000000
VERSION     DASVEV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615355"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..709
                     /locus_tag="VF222_07490"
     CDS             467..709
                     /locus_tag="VF222_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142304.1"
                     /translation="MANSLSFPDLEISRNKTMLSPESKYQELKQQWYFHPSSWYNRKK
                     GEGSYDGKGCNQFTGCIPECKFYPENGRLAENEEFD"
     gene            1061..1801
                     /locus_tag="VF222_07495"
     CDS             1061..1801
                     /locus_tag="VF222_07495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7142305.1"
                     /translation="MIRDKVVIVTGASSGIGYATALALSKAGAKVAAGARRVEKLQTL
                     KNEVQENGGEILIKKLDVTKKEDCDSFINLVTKTWNHIDILINNAGIMPLSFFKNLKI
                     SEWEQMIDVNLKGVLYCTAAVIPYMITKKTGHIVNISSVAGRMVFPAGSVYCATKHAV
                     TAFSEGLRQELSQRYNIKITCIEPGVVSTELPNTITDKSLEPFIESVKKMESIKAEDI
                     ANAIIYSLDSPQYLNINEILLRPLSQEK"
     gene            complement(2836..4449)
                     /gene="pckA"
                     /locus_tag="VF222_07500"
     CDS             complement(2836..4449)
                     /gene="pckA"
                     /locus_tag="VF222_07500"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX7142306.1"
                     /translation="MAKDSEKGNLKSLAMMKKFGFYPKKLYSQLSFPELVEYSIQRNE
                     AILSHTGSLSVETGKFTGRSPDDRYIVQDKMTTNTVNWGKVNHPISEENFNNIFERMK
                     KFVKNKEIFLFNGFVGSDEDVRLPISIVTDRAWQSIFANQIFIKPTKVDIEKYKPKVL
                     LVAFNDFKSIPKKDGTKSETFIVINLKRRIILIGSTSYAGEIKKSIFSLMNYYLPEQG
                     IFPMHCSANVGKNNDSALFFGLSGTGKTTLSADSNRFLIGDDEHGWSSKGIFNFEGGC
                     YAKCINLKKETEPQIWNAIKFGSVMENVVVTKDKRIPDFSNGSLTENTRVVYPLEFIP
                     GAVIPSLAGHPKVIVFLTADAFGVMPPIAKLSKEGAMYHFMSGYTSKLAGTERGITQP
                     KETFSQCFGAPFMPLHATKYAEMLGNKITQYNTKVYLINTGWYGGPYGIGKRIDLKYT
                     RSMVTAVINGSLMKRNSFKTHQIFNLDIPLECPGVPNELLDPVLTWDNQEEYFQAATK
                     LANLFIQNFEKFGEVPKKIIEAGPQKINL"
     gene            complement(4609..>5017)
                     /locus_tag="VF222_07505"
     CDS             complement(4609..>5017)
                     /locus_tag="VF222_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142307.1"
                     /translation="KLKQKIEKEMKIGSRIVTILSPPGLMLPSQVDYPFIMCKKPFTY
                     ASDLKQQIKSIYGNSCIDFTASWILSEKYIKKLETPTKYARFINILLSMIIWINSWNV
                     GVACEKEIPPPVETYIGILRTFFNIDLTEMIKK"
BASE COUNT         1728 a          776 c          759 g         1754 t
ORIGIN      
        1 tcttttcgag ttctgtaagc cagttattac ttaggaatct aaagaatgca aattcgctca
       61 taagataata tgcattatct tctagttaac tttatcttat ttgacttttt acatgacgat
      121 aaataatcaa tagaataaga atacttattg ttggaattaa atacattaag ttatgtaatt
      181 agtattactg gaattgataa tttagtcgag aactatttta aaaaggtata atcttacttg
      241 aatattagtc tctttcacgt agataatgaa gcctggcttg caacacacac cattcctccc
      301 taggatttta aaaattccct tctaaggttt ttatcaaata aataaatgaa ccttaacaaa
      361 aaaaggtaaa aaaagtatat gcgattattc gcaatttttc tcaagaatgt tgtaatagat
      421 caatcctacc ttcttcatat taagtagatt ttatatctat aatctcgtgg caaacagttt
      481 atcatttcca gatttagaaa tttctagaaa taaaacaatg ttaagtccag aatcgaaata
      541 tcaagaatta aaacaacagt ggtattttca cccaagttct tggtataaca gaaaaaaagg
      601 tgaaggtagt tatgatggaa aaggatgtaa tcagtttaca ggatgcattc cagaatgtaa
      661 attttatcca gaaaacggaa gacttgctga aaatgaagaa tttgattagt actattacta
      721 tactttctat tttcattttt ttatgaaata gaaatataat aatatctata ttttaataat
      781 ttttctaaat aaaatttgca agccctttaa aataggctat actcttggtt tcgaagaatg
      841 gtattcacac tccttaaacc attatttgaa ttcaaaacga aagtataaaa atttgattat
      901 tatgactaat attcatagta aatcaaagct aaaattgtgt gtttatcatt atttctctat
      961 cgtgactgcg ttgtataaga tgcctaagaa ttttactaac ttatttttaa tataataaca
     1021 aaagaaatta aaggtataca tttagtttta ctatataatt atgataagag ataaagttgt
     1081 aattgtaacg ggggcgagta gtggtatcgg ttatgcaact gctttagcct tatcaaaagc
     1141 tggtgcaaag gtggccgcag gagcccgaag agtggagaag ctacaaactt tgaaaaatga
     1201 agttcaagaa aatggtggtg aaatactcat taaaaagctt gatgttacta aaaaggagga
     1261 ctgtgattct tttattaact tagtaactaa aacatggaat catattgata ttctaattaa
     1321 taatgctggt attatgcctc taagtttttt taaaaatctt aaaataagtg aatgggaaca
     1381 aatgattgat gtaaatctaa aaggagtatt gtattgtact gcagctgtta ttccatatat
     1441 gattacaaaa aaaactggtc atattgttaa tatttcatca gtagcaggaa gaatggtttt
     1501 tccagcagga agtgtatatt gtgctactaa acatgctgtt acagcattta gtgaagggtt
     1561 aagacaagaa ctaagtcaac gttacaatat caaaataaca tgcattgaac ccggagttgt
     1621 ttctacagaa ttaccaaata caattactga taaatcttta gaaccattca ttgaatcagt
     1681 aaaaaaaatg gaatccataa aggcggagga cattgctaat gcaattatat attcacttga
     1741 ttcaccacaa tatctcaata ttaatgaaat attattaagg cccttgtctc aagaaaaatg
     1801 atttgttaag attcgttttg aatatattcg cgaaaagtaa ttcaaaacag agattgtata
     1861 caaaagttgt cataccaaac tagaaaatca agttaatttt gagttttatt tagtgaccaa
     1921 tgctaatcta aatgccaatt ttaacttaaa atcaatgtca cctgctgtga ataataataa
     1981 cacatttgag ggatacaatt accatatttt aacccttctg cttatgtttt ctttatgtat
     2041 ggggattaac attataactt aatataaata cttagattct attatccaat ttaccatctt
     2101 gaagagaaaa aaccgaagag acatagaacc ctataaatga tggactatac cagtttgtac
     2161 aattaatcca tatcaaaact agaaaaggaa aacctaacac aagttcgtga atccaattca
     2221 tcataatatg atatatctac cttatgaatt ttcattttat caatatttta tgaacttttg
     2281 taagtgcgat tgatgataaa tactatatta aatatagcgt tctatgctgg agtgatttaa
     2341 acttaatagt aaatatgaat ccaagtcaag cagaaaaata tatacctgcc aattggttat
     2401 gcccttggtg gtagatatca actagtacta gcatgtgatg tatgcattgc ttcatcaaat
     2461 gctaaaattg gccaaaatct ttaatatgaa ttaaatgtat tgctctgtat cgccaatgat
     2521 ttaatggtaa tataatgtta aaaaattatt tatctcgatt cgattttata ctctagagat
     2581 tgttataatc taaagctgtt aatacttgta ggtatactaa ttactaaaaa tctaaataat
     2641 taaaaaaatc tattcgataa aatcaaatat cggaaagagt aataaattgt atagctcgag
     2701 aagcccggtc gaatcagttt ttcttagaga gaactgatgc caagtctagc ggctaaactc
     2761 cttgtagggc tagggatttc aggctctgga tctctgaaaa gaaacctgga gacagcagtt
     2821 cgaatctgct ccgggctata aatttatttt ttgaggccca gcttcaatga ttttcttcgg
     2881 aacttctcca aatttttcaa aattttgtat aaataaattg gctagttttg tggctgcctg
     2941 aaaatactct tcctgattat cccatgtcaa tactgggtct aataattcat ttggtactcc
     3001 aggacactct aaaggaatat ctaaattaaa tatttgatga gttttaaagg aattcctctt
     3061 catcagagat ccatttataa cagcagtaac catagatctc gtatatttca aatcaatcct
     3121 tttgcctatt ccataaggtc ctccatacca accggtattt ataagatata cctttgtatt
     3181 atactgcgtt attttgtttc ctaacatttc tgcatatttt gttgcatgga ggggcatgaa
     3241 tggagcacca aaacattgtg aaaaagtttc tttgggttgt gtaattcctc gttcagtacc
     3301 ggctaatttg ctggtatatc ctgacatgaa atgatacatg gcaccttctt tactcagttt
     3361 agcaattggt ggcatcactc caaaagcatc agcagttaaa aatactataa ccttaggatg
     3421 acctgctaaa cttggaataa cagcacctgg aataaattct aaaggataaa caacacgggt
     3481 attctcagta agtgaaccat tagaaaagtc aggaatcctt ttatcttttg taacaactac
     3541 attttccatt actgacccaa actttatggc attccaaatt tgtggttctg tttctttctt
     3601 caagttaata catttcgcat aacaccctcc ctcaaaatta aagatacctt tagaagacca
     3661 cccatgttcg tcgtctccta ttaaaaacct attagagtca gctgataggg ttgtttttcc
     3721 agtccctgat agtccaaaaa ataaagcaga atcgttattc tttccaacat ttgcagaaca
     3781 gtgcataggg aatatacctt gttctggcaa ataataattc attaatgaaa aaattgattt
     3841 tttaatttct ccagcatagg acgttgaacc gattaagatt atccttctct ttaaatttat
     3901 aactatgaaa gtttcagatt tagttccatc tttttttggt attgacttga agtcattaaa
     3961 cgcaactaaa agtacttttg gtttgtattt ttcgatatct acttttgttg gttttataaa
     4021 tatttgattg gcaaaaatag attgccatgc cctatcagtt actatgctta taggaagcct
     4081 aacgtcctca tcagaaccaa caaacccatt gaataaaaaa atctctttat tttttacaaa
     4141 ttttttcatt ctctcaaaaa tattgttgaa attttcttca gagatgggat gattaacttt
     4201 tccccagtta acggtgtttg ttgtcatttt gtcttgaact atatatctat catcaggaga
     4261 tcttccagta aatttaccag tttcaactga taacgaacca gtatgagaca aaattgcttc
     4321 atttctttga attgaatatt ctacaagctc cgggaacgat aactgcgaat ataatttttt
     4381 tggatagaac ccaaattttt tcatcattgc taagcttttc aaatttcctt tctctgaatc
     4441 cttggccatt aggatacctt tacggattat tattagcatt atttaattga tttcctttta
     4501 caaagaacac ctctctacat tcatcttgag atttttattt aataaaatct ttattgactt
     4561 ctcatcacag aattatatag aaggcaatac taggtattgt gaacaattct atttttttat
     4621 catttctgtt agatcgatat taaaaaaagt tcgtaatatt ccaatataag tttcaacagg
     4681 gggaggaatc tccttttcac aagcaactcc aacattccat gaatttatcc agattatcat
     4741 actcaaaaga atatttatga atctcgcata cttcgttggt gtttcaagtt ttttaatata
     4801 tttctcagag agtatccagg atgctgtaaa gtcgatacac gaattgccat aaattgattt
     4861 tatttgttgt ttcaagtctg atgcataagt gaatggtttt ttacacatta taaaagggta
     4921 gtcaacttgg gatggaagca taagtccagg aggagataat atcgtaacta ttcgagaacc
     4981 tattttcatt tctttttcaa ttttttgttt tagttta
//
LOCUS       DASVEV010000165         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_836802, whole genome shotgun sequence.
ACCESSION   DASVEV010000165 DASVEV010000000
VERSION     DASVEV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836802"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF222_07510"
     CDS             <1..299
                     /locus_tag="VF222_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142308.1"
                     /translation="FGKIVYDLTFEYKCKLDSVIEDCWKFKALDLLDISDELTNDEKQ
                     KIIEHLFNQISQNKVEQMKKKEKEKENDIDPSNEKVLLPYIQYLQNRSLSSKKI"
     gene            370..1350
                     /locus_tag="VF222_07515"
     CDS             370..1350
                     /locus_tag="VF222_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142309.1"
                     /translation="MYSKNSVNEKLINLIENSGFIYRNQITDIVIRDIILNVLNRIGK
                     YYSKALIYQICSLYGLSQNELLSNYELFENSLTRVLGKTGYPIILRIKKELLKYLILN
                     KFEISTKDILDPSFNVPQIIDKLSESYNYQLISENLNNNRNHMILLSSNDDKHIEKII
                     TNLVQISKNIRDIKRVVVVLHDQFDEKRYFSSVPDIDIYYMNDIFSSSASSSSVEEFF
                     KDNNNKNDLINFEQFNNTNIQVFCFIDIQILRQFNNYKILKILEDKFEQLYNLNEIIQ
                     ICCIYDVSNFYTKEYEIKLLRDFINHHNMVIIDEPLVMYQKFDVKNDIVK"
     gene            1347..2144
                     /locus_tag="VF222_07520"
     CDS             1347..2144
                     /locus_tag="VF222_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142310.1"
                     /translation="MNLQSFYKFRKRFLFIVGLSLLSVILLISLSPENQKENVGNITN
                     PISIIIAASLSVLILYRQKTDGEIGKAFLMLTFGLVVWAVAEILFTYSGIITLELERN
                     IMLSDLLWLIGYGPLIYYSFKMYVLFHNYSSKKSIVVILIMMIAYLVYILPFISDTYS
                     SSSNEDLPLLYISISYLVLDLVFIFPASLVILNLGIFNNKNQGVLTFFPWMFVAMIII
                     GIADSIFAYTSVSGYYQLEWITTPIYTSGYLLMAFGLYWHYKFFIYK"
BASE COUNT         1044 a          308 c          339 g         1002 t
ORIGIN      
        1 catttggaaa aatagtttat gatttaactt ttgagtacaa atgtaaatta gattcagtta
       61 tagaggattg ttggaaattc aaagctctag atttgttaga tatatcagat gaattaacca
      121 atgatgaaaa acaaaaaata atagaacatt tgtttaatca aattagtcaa aataaggtgg
      181 aacagatgaa gaaaaaggag aaggaaaaag aaaatgatat agatccatct aatgaaaaag
      241 tgcttcttcc ctacatacaa tatctgcaaa atagaagttt atcttcaaaa aaaatttgaa
      301 tcctttttaa taatctaatc ttgatataca tattaaaaaa taataaataa ataaataaat
      361 aaataaactt tgtactctaa aaattcagta aatgagaaat taataaatct tatagaaaat
      421 agtggattta tttatagaaa ccaaataacg gatattgtta ttagagatat aatattaaat
      481 gttttgaaca gaataggcaa gtattattct aaagccttga tatatcaaat atgcagtctg
      541 tatggtttga gtcaaaatga attattatcc aattatgaat tatttgaaaa tagtttaaca
      601 agagttttgg gaaaaacagg atatccaata atccttagaa taaaaaaaga attattgaaa
      661 tatctcatac tgaataagtt tgagattagc acaaaagata tcctggatcc ttcctttaat
      721 gtacctcaaa taattgataa attatctgaa tcttataatt atcaattaat atctgaaaat
      781 ctaaataata atcgaaatca tatgatttta ttatctagta atgatgataa acatatagaa
      841 aaaattatta ctaatcttgt tcagatttca aaaaatatta gagatattaa aagagtagta
      901 gtagtattac atgatcaatt tgatgaaaaa agatatttca gctctgtacc tgatattgat
      961 atctattata tgaatgatat attttcttct tctgcttctt cttcttctgt tgaagaattt
     1021 tttaaagata ataataataa gaatgacctt atcaactttg aacaatttaa taacacaaat
     1081 attcaagttt tttgttttat agatattcaa atattacgcc aattcaataa ctataaaatc
     1141 ctaaaaatac ttgaagataa gttcgaacaa ttatataatc ttaatgaaat tatacaaatt
     1201 tgctgtatct atgatgtatc caatttttat acaaaagaat atgaaataaa actattaagg
     1261 gattttatca atcatcataa catggtaata atagatgaac cattggtaat gtatcaaaaa
     1321 tttgacgtaa aaaatgatat tgtaaaatga atttacaaag tttttataaa ttcagaaaaa
     1381 gatttttgtt tattgtaggt ttatctttat tatctgttat attactaatc tctttatctc
     1441 cagaaaacca aaaggaaaat gtaggtaata ttacaaatcc tatttcaatt attattgccg
     1501 cctcattgtc ggttttaata ttataccgac aaaagactga tggcgagatt ggaaaggctt
     1561 ttctaatgtt aaccttcgga ttagtagtat gggctgtagc tgaaattcta ttcacttatt
     1621 caggaatcat aacattggag ttggaaagaa atatcatgtt atctgatttg ctttggttaa
     1681 ttgggtatgg acctctaatt tactatagtt tcaagatgta tgttttattt cataattatt
     1741 caagtaaaaa aagcatagtt gtaattctta ttatgatgat cgcttattta gtctatattt
     1801 taccttttat atcagacact tatagttcct catctaatga agatctgcca ttattatata
     1861 ttagtatatc gtaccttgta cttgatttag tttttatttt tcctgcatct ttagtaattt
     1921 taaatttggg aatttttaat aataaaaatc aaggagtttt aacatttttt ccctggatgt
     1981 ttgttgcaat gattataata ggaatagcgg atagcatttt tgcctatacc tcagtctcgg
     2041 gatattatca attagaatgg ataacaactc ctatctatac ttctggatat cttctcatgg
     2101 cttttggtct ttattggcac tataaatttt ttatttacaa atgaaaaaaa tataaaattt
     2161 tgctatatct cctcctcctg ttctatcctt aatattttaa tttgtattac ataaataata
     2221 attgatattt aatatttata aaataacatc atttcatata gaaacataat ttcttctttc
     2281 gtcgttgaaa attactaatg cattagacat aatacataga ctaagtaaaa ttatattaga
     2341 aatattctac atattattta aaatagaata aaattaattc tttcatttca ttataacaac
     2401 aaatttaata taaaaattta attcaagaca tttacaactt aaaatattta ttaaaaacaa
     2461 gtttaattat atatattgat tacggcagaa agtgcttgcg tactaaccgc tgaaatgatt
     2521 ggaatagtat tttataatta ttctattgtt tctcaattcc agtagcatcg ttaataggag
     2581 agccttaaca atagtattca ttaaagcatt aaaaaaataa aagtccaatc acaatcttgt
     2641 gatagttgta attgcagtga tgtatgatag acgattaagt agtattttct ata
//
LOCUS       DASVEV010000166         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_450543, whole genome shotgun sequence.
ACCESSION   DASVEV010000166 DASVEV010000000
VERSION     DASVEV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450543"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..448)
                     /locus_tag="VF222_07525"
     CDS             complement(299..448)
                     /locus_tag="VF222_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142311.1"
                     /translation="MMISDHLKYTSLFKTYDPFNYCKDVGDKVCDHTLYGQTEEELFE
                     NVKED"
     gene            930..1115
                     /locus_tag="VF222_07530"
     CDS             930..1115
                     /locus_tag="VF222_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142312.1"
                     /translation="MEDCRCNKYDKTSDLLLDIFIVSSHLKLLRCVKCGGLIGEWTDK
                     ITPIKRSYSKKEQELLK"
     gene            1223..1486
                     /locus_tag="VF222_07535"
     CDS             1223..1486
                     /locus_tag="VF222_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142313.1"
                     /translation="MANKDSCGSHGTAYRKNKTFEECKEMALLNSKLLSSEINEQGEI
                     AWSRVLTVANNDELVYKLTLKYLRQRGFDIGNNKTPRVKLLVD"
     gene            complement(1747..>2161)
                     /locus_tag="VF222_07540"
     CDS             complement(1747..>2161)
                     /locus_tag="VF222_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142314.1"
                     /translation="FILLGAGMGIFTSPNRASIMNSVPANRRGVSASTGTTLFYVGRS
                     LSLGISFLIMTSILPAEQVKEIFIDFRNTEANMIKDNTNNNTNIPSTTTTITENKELI
                     ENKFLYSIHIIFFVSSIIVFISIIPATVKDRNIRR"
BASE COUNT          815 a          248 c          274 g          824 t
ORIGIN      
        1 gatatctaaa gataatcata gcagaagaat agtgaatatc taataactag ttcaaatact
       61 aaaataagaa aaaatgctaa actaaagata cctatccttt atcaattgtg tctagtaaaa
      121 atcaaatatt attattataa atgatttttg ataaacataa tattagtaat tattttagtg
      181 aagcagtttt aaatattata atgaatatta ataaataaat aattttaaat ttcttttatc
      241 aacttttgaa atgttctaaa ttgccaaaaa tttcttcttt ccaagtctct tctgtatatc
      301 aatcttcttt cacattctca aataattctt cttcagtttg gccatacaag gtatgatcac
      361 atactttatc tcctacatct ttacaataat tgaaagggtc atatgtttta aataaactag
      421 tatatttaag atgatctgaa atcatcatat caataataaa agttagttaa attttaaaaa
      481 tagattgaat ctatgatcga attttgcaat tacatatgac tacattatat atttttaatt
      541 taaactaaat ttaaaatcta atgaaaatgt tagtttatga tcaagatcat ttgccaacta
      601 atttaaagtt cctgcttgtt taaataatat tttggatttg attataaata aaaattttat
      661 agaatattcg tataagctaa ataaaagtaa cattaattga taattattta ttgatactca
      721 gcaagttgtt gaagattcat tcatttgtcc ttacataatt ggtgatggat gctgattgat
      781 ttatcaatca gatgtagaga tctagagata caagttcaag cacttaagtt gatcaataac
      841 aaatttagaa ctatgtcaat gtttgctatc tttgatccat agtttttaat caaaactatt
      901 tttaatttta ttggtcgtat atatatcaga tggaagattg tcgatgtaac aaatatgata
      961 aaaccagcga tttactttta gacatattca ttgtatcttc tcatttgaaa ttattaagat
     1021 gtgttaaatg tggcggatta attggtgaat ggacagataa aattactcca ataaaaaggt
     1081 catattcaaa aaaagaacaa gaactattaa aataatattt gaaagtgagt tttcaattat
     1141 ttgcattata aagactaacg tcttattatt tgtatttgaa aagaaaatta gatatgtatc
     1201 tatattcaaa taaatataat aaatggctaa taaggattcc tgtggctctc atggtacagc
     1261 ttacagaaaa aataaaacct ttgaagaatg taaagaaatg gctttactaa attccaaatt
     1321 attatcatca gagataaatg aacaagggga gatagcgtgg agcagagtac ttacagtcgc
     1381 aaacaatgat gaattagttt ataaattgac tttgaaatat cttcgacaaa gaggattcga
     1441 tattggcaac aataagactc caagagtaaa gctattggtt gattaaaata aatttttttg
     1501 tttatgtctt aagtttgatc aactcatgta aaattatata tctgatcatt taattatttt
     1561 ttgttaatga tgtattaaag aaattaaaga ggtcctgttc tcaatgaagt taagtcttaa
     1621 ttaagttttt ttgatttgtc tcattattaa aataaaagat gttctcattt ttgtaatttc
     1681 ttttcatttt ttaaagcttt atttaataaa aaagatgaaa taatattgtt ttattacttc
     1741 ttagatttat cttctaatat ttctatcttt aactgtagca ggaattattg aaataaaaac
     1801 tataatagaa gaaacaaaaa atattatatg tattgaatac aagaacttat tttcaattaa
     1861 ttctttattt tctgttattg ttgttgttgt tgaaggaata ttagtattat tattagtatt
     1921 atctttaatc atatttgctt cagtatttct aaaatctata aaaatttctt ttacttgttc
     1981 tgctggcaat atgcttgtca ttattaaaaa tgagatacct aaactcaaag accttccaac
     2041 ataaaataaa gtagtacctg tacttgcaga tactcccctt ctatttgcag gtacactatt
     2101 cattatggat gctctattag gagatgtaaa tatacccatt cctgctccaa gaagaataaa
     2161 a
//
LOCUS       DASVEV010000167         6929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_60613, whole genome shotgun sequence.
ACCESSION   DASVEV010000167 DASVEV010000000
VERSION     DASVEV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60613"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VF222_07545"
     CDS             <1..766
                     /locus_tag="VF222_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7142315.1"
                     /translation="FAVGEYVLGMLGWREYWLSNGLDVIKVNPKLAPIQSFLGTFGMT
                     GLTAYVGLLKIGELKQNDTIFVSAASGAVGSIVCQIAKINGCHVIGSAGSDEKVKWLV
                     DQAGIDYAFNYKQMNDISAELLKVCPDKIDIYFDNVGGKHLEAALDNMKTFGRIVFCG
                     MISQYNANSPIPGPSNLFLAVTNRLKLQGFIVRDHYDLLNEFHNAMTNWVSEGRIKWK
                     ETILEGIENAPKAFLGLFKGENFGKMLVKIGPDPAI"
     gene            1667..1840
                     /locus_tag="VF222_07550"
     CDS             1667..1840
                     /locus_tag="VF222_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142316.1"
                     /translation="MSNIAATYIGIVAGFALGGLVSWWIYNRQEKTAVKQQEILDHIV
                     NLEEKHDYILKKG"
     gene            1865..2167
                     /locus_tag="VF222_07555"
     CDS             1865..2167
                     /locus_tag="VF222_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142317.1"
                     /translation="MVIKQKRAITMIILFYVSLLMTPTVYAAIEKVEKYAADICIQTY
                     KGDWDGADCDIQEAEDEQQYYDDIASLEDSICEEVVKDVSPEDLIDLCKNLNVFEK"
     gene            2716..2868
                     /locus_tag="VF222_07560"
     CDS             2716..2868
                     /locus_tag="VF222_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142318.1"
                     /translation="MSENEETSSSERVKGDEYVLKKANQANLKEEEEKDRTVKVDTKE
                     DTTSEE"
     gene            3358..4110
                     /locus_tag="VF222_07565"
     CDS             3358..4110
                     /locus_tag="VF222_07565"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7142319.1"
                     /translation="MGNKFEGKIVVITGGNSGIGLATAKRFVSEGAYVFITGRRQNEL
                     DAAVLEIGKNVVGIQGDVSNLEDLDRLYNTVKDQKGHIDILFANAGIAQFAPLGEISE
                     EHFDNIFRINVKGVLFTVQKALPMFHDGGSIVLNSSIGSSKGVEESSVYSATKAAVRS
                     FARTWTVDLRHRQIRVNAISPGPIDTPIFSNLLQNEEQSEQFKKNIVNTVPMGRMGSP
                     DEIAKVVSFLASDDSSYITGIELFVDGGLAQI"
     gene            complement(4336..5853)
                     /locus_tag="VF222_07570"
     CDS             complement(4336..5853)
                     /locus_tag="VF222_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142320.1"
                     /translation="MKNIKEKDNKDQQKEIDTKISPYAWKTLSLLGIIAIMVMYAETM
                     LIPAIPDIITDLNISFGTSSWILTAYLISGAVMTPICASLSDIYGKKKILLIVVFIYL
                     IGTTIAYFVSDIVTFIIARAIQGIGISMFPIAFSIIRDCFPRKKISIGQGVITSMFAT
                     GAAVGFSIGGLVVHNYGWHFTFLTLIPPTILFIILVWKYIDVKKIAEYKQLNHNINKD
                     NAENLSTKVDIKGALYLSIAITSFLLALTLFENNISDSKIQSIIILLFIISLSTLATF
                     IIIERRTDQPLINFKLFTNKTILSANLIILLIGFTHFMIFQSIPILARNPEPLGFGLD
                     AVNTGNIQLPFAITFVLFGPASGIIISRMGSLKPVILGTIISSISFLFMLFFHTEEYY
                     ISVALAVFATGLSFTAIGAMNIIILFTPSNYIGTSTGLSVLIRILGSSIGPVIAALYM
                     QLNQSHIAMNESYFYPSSYSFDLIFLTATIASICSIVLAFYLKRITNEKITTVSD"
     gene            complement(6121..6507)
                     /locus_tag="VF222_07575"
     CDS             complement(6121..6507)
                     /locus_tag="VF222_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142321.1"
                     /translation="MEENQCESNCECVETQRSSGHHNDANECCYCQTTTANKTNSNSG
                     QCCSDDPIAAAKSLLERSFFTALREVHVEKLKKQIEREWGSTIDKAVDLTIKTVAKQW
                     QASLSKSAVNKEFYSELEKIFKSAKE"
     gene            6595..>6929
                     /locus_tag="VF222_07580"
     CDS             6595..>6929
                     /locus_tag="VF222_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7142322.1"
                     /translation="MTEQKLLDEDNNRETSQFMLDGMNCKCCPVFNTLSIIGKKFTLL
                     LLRNMIFLKQKRFNEFLNSIEEINPKTLSIRLKELERDRLIKREVFNETPVRIEYYLT
                     EKGKALQPI"
BASE COUNT         2410 a          978 c         1150 g         2391 t
ORIGIN      
        1 atttgctgta ggtgaatatg tcttaggaat gcttggttgg cgtgaatatt ggttatcaaa
       61 tggtttagat gttattaagg tcaatccaaa gttagctcca attcagtcat ttttaggaac
      121 atttggtatg acaggtctta cggcatatgt tgggctttta aaaattggtg agcttaaaca
      181 gaatgataca atatttgttt cggctgcatc aggcgcagta ggttctattg tatgtcaaat
      241 cgcaaagatc aatggttgtc atgttatagg aagtgctggc tcggacgaga aagtaaaatg
      301 gcttgttgac caagcaggaa tagattatgc ctttaattat aaacagatga atgatatttc
      361 agccgagctt ctaaaggtat gtccggataa aattgatatt tactttgata atgtaggtgg
      421 aaaacactta gaagcagccc ttgacaatat gaaaacattt ggcagaatcg tattttgtgg
      481 tatgatatca caatataatg ccaattctcc cattccaggt ccttctaacc tcttcttggc
      541 tgtaacaaat cgactgaaac ttcaaggatt tatagtaaga gatcattatg atttgctaaa
      601 cgagtttcat aatgccatga ccaactgggt tagtgaagga agaatcaaat ggaaagaaac
      661 aattttagaa ggcatagaga atgcaccaaa agcttttctt ggacttttta aaggagaaaa
      721 ctttggcaaa atgcttgtaa agattggacc agatcccgct atataagaca tggcaatttg
      781 tgtagtgaac tcctagtttc gccttgagtg ggaattaaag gcaagcatgg agtattgtca
      841 gtttctacta aagccgctac gataacctag cattattgat ttatttactt agaagttaaa
      901 agccatagcc ctcttctact actaatgagg agccagacaa catagaagaa ggctatggtg
      961 acgttagtct tgcctttcgg agtgttttat caaactatat tatagtattt aataattaga
     1021 tataatttaa ttgtcatagc ctgaactggt agaagaatcc atctgatgat tcagactatg
     1081 acaacaaaca tttggttaca cttgatcagg tgtctctaaa ttttgggttt atgagtaaat
     1141 tacatattgt gtagaatata tttattcata tcttaaattc tcattctatg ttttgaaatt
     1201 aatattcatt ttaataatta caatatgttt ctgttattag taataataat taataaagag
     1261 atgaggttta aggtctatct caacggttga ttgtcaaaac aatacgctgt aactgtaaat
     1321 attttagtgc caggaccagt aaatcatatt tagatatcga gattatatga ccgaggacac
     1381 tcttccatat aacctctagt gaaatcaccg atttcacatt aattatatat gcactttgga
     1441 ttatttattt atacctaact ttgtttgttt atacccaatt attataaaga gcagattgtt
     1501 ctatattatt agcttgcaac ggtgcaaacc ctagaacatt gttccttttg tttatttttc
     1561 ataggaataa ataaaaagtg aagtatcgaa tccagataat atggtattag agttgttctt
     1621 aaattgagga tgtatagaag catttcattg taatgaattt ggtgacttgt ccaatattgc
     1681 tgctacgtat attgggattg ttgctggatt tgcattagga ggtttagtta gttggtggat
     1741 atacaataga caagaaaaaa ctgcagttaa acaacaggaa atactagatc atatagtaaa
     1801 cttagaagaa aagcatgatt atattttaaa aaaaggataa aagtaactat attgataaca
     1861 taatatggtt ataaaacaaa aacgagccat tactatgata atactatttt atgtttcgct
     1921 gcttatgaca cccacagtat atgctgcaat agaaaaagta gaaaagtatg ctgcagatat
     1981 atgtatacag acttataaag gtgattggga tggcgccgat tgcgatattc aagaagcaga
     2041 agatgaacaa caatattatg atgatatagc aagtttagaa gattcaatat gtgaggaagt
     2101 ggtaaaagat gtaagccctg aagatttgat agatctatgt aaaaatctca atgtatttga
     2161 aaagtaacta atcttttcta ctggattaaa atgtatttaa tatgaacctt ttttaatttc
     2221 aatttttaat aaaactgaat acagcattat tatattttct tatacttgtt ttactatact
     2281 ggctaactta aattcacgta tctttaaatt ttagaaaaac atacttttta aacataataa
     2341 acatacttta tttgatagaa tttgtaagcg aattttttgt tagttagtat agtgtggttt
     2401 ttactaatca gttcgccact gaagagttgg tatgtcgtat atccttatta tgtatttaga
     2461 agttttgtca ttaattctga ttaccaattt catctttagc catttctggc attattatta
     2521 gataaattta ttataaatca tatccaatgt attacttttt tatcgattaa gaaataaaaa
     2581 tgaatatatt tattattagt atatgaaact ttttttataa aagatacttt cctttaacca
     2641 attttaaaat tctttatctg ataatttttg ttttgatatt tttaaatagt ataatattat
     2701 aaatatttca tagatatgtc agaaaatgaa gagacatcat catcggaaag agtaaaagga
     2761 gatgaatacg ttctaaaaaa ggcaaatcag gcaaatctca aagaagagga agagaaagat
     2821 aggactgtta aagtagatac aaaagaagac accacttctg aagaatgaga aaccttacat
     2881 atagataaaa gaaaaaaata tagaataaat aaaaaatttt caaaaatcaa tcaatttatt
     2941 ttctttttaa ctgcttactg tcatattctt tcacttacga taagtaatga taaaataaat
     3001 gagataatgt atcatattta ccttggcatt ctctcttgtt aactaattat tatatctagt
     3061 ctttgatttt tatatttcga attaaaaata acaatacttt ctgaactata aaattgtgtc
     3121 aaaatcagtt cacacacctc tgtttgcacg cttatgaatg gtttattagt atgttaacaa
     3181 acttcaaagt aaaaatataa ttttcataat tataatcttt acaaatactg taatttttgt
     3241 atataagtaa ctcttcctct tttaacaact taccatcaat tcacttgata accacattgt
     3301 tcccacctaa tttatattgt taccttatgt tatcgaataa caataagtga gttaaaaatg
     3361 ggaaataaat ttgaagggaa aattgtagtt atcactggag gaaacagtgg tattggttta
     3421 gctactgcaa agagatttgt atcagaaggc gcatatgttt tcattaccgg tcgccgtcaa
     3481 aatgaacttg atgcagctgt attagaaata ggtaaaaatg tagtaggtat tcagggcgat
     3541 gtatcaaatc tcgaagatct tgatagattg tacaatactg tgaaggatca gaaaggtcat
     3601 attgacattc tatttgcaaa tgcgggcatt gcacaatttg ctccattggg agaaatttcc
     3661 gaagaacact tcgacaatat attccgcatt aatgtaaaag gagttctgtt tactgtccag
     3721 aaagcacttc ctatgtttca cgatggagga tcgatcgttt tgaattcttc aataggttca
     3781 tccaaaggag ttgaggaatc tagtgtttac agtgcaacca aggctgcagt acgttcgttc
     3841 gctcgaacat ggacagtcga tctgagacac cgccaaatcc gcgtcaacgc catcagtcct
     3901 ggtccgatcg acacacctat ctttagcaat ctactgcaga acgaagaaca gagtgagcaa
     3961 ttcaagaaga atatcgtgaa cactgtacct atgggaagaa tgggcagtcc tgacgaaatt
     4021 gcaaaagttg tctcatttct tgcatcagat gatagcagtt atatcactgg tatagaacta
     4081 ttcgttgatg ggggtctggc acaaatctag atagtatcct ttttcatcct gctttttcat
     4141 gcgattctga atctagtagt aagtaaaggt ttattacaaa aaatattagc taatatgttt
     4201 ccaagagata ccagcgttag attgcatatt ctaattatga aatcatatta ttaaatatcc
     4261 caagatagta attgtggtag tattaacaag atactagata atatactaac caaaataact
     4321 tctggccagt cttatttaat cagatacagt agttattttt tcatttgtta tcctctttaa
     4381 ataaaatgct aatactattg aacatataga tgctattgta gcagttaaaa atataagatc
     4441 aaaagaataa gatgatggat aaaagtatga ttcattcata gcaatatgag attgattaag
     4501 ctgcatatat aatgcagcga tcacaggccc tattgagctt cccaatattc ttattaggac
     4561 acttaatcca gttgatgttc caatataatt tgaaggagta aagagtataa ttatgttcat
     4621 tgcacctatt gcggtaaatg ataatcctgt tgcaaatact gccagagcta ctgaaatgta
     4681 atattcttct gtatgaaaaa ataacataaa aaggaaacta atactagaga ttatagttcc
     4741 tagaattaca ggcttcagtg atcccattct tgatattatt atacctgaag caggtccgaa
     4801 taatacaaaa gttattgcaa atggtagttg gatatttcct gtattaactg catcaagacc
     4861 aaaacctaaa ggttcaggat ttcttgctag tataggtata gactgaaaaa tcataaaatg
     4921 ggtaaatcca ataagtaata ttattaaatt agcagaaagt atagttttat tagtgaagag
     4981 tttaaagttt atgagaggtt gatctgttct tctttcaatt ataataaatg tggctaatgt
     5041 tgatagagat attataaata atagtattat tatactttgt attttagaat cggagatatt
     5101 gttttcaaat aaagttaatg ccaatagaaa ggaagtgatg gcgattgaaa gatataaggc
     5161 tccctttata tccaccttcg tagataaatt ttcagcatta tctttattta tattgtgatt
     5221 caattgttta tattctgcga tttttttaac atctatatat ttccatacaa gtataataaa
     5281 aagaattgtt ggtggtatta atgttagaaa agtaaaatgc catccataat tatggacaac
     5341 taatccacca atagagaagc ctacagctgc acctgtagca aacatagatg taataactcc
     5401 ttggccgatg gatatttttt ttcgaggaaa gcaatctcta attatactaa atgcgattgg
     5461 aaacattgat attcctattc cctgaatcgc tctagcaatt ataaaagtca ctatatcaga
     5521 aacaaaatat gcaattgttg tgccaatcaa atatatgaac acaactatta ataaaatctt
     5581 ttttttacca tagatgtctg ataaacttgc gcaaatagga gtcattaccg ctcctgaaat
     5641 caaatatgct gtaagaatcc aagaagatgt tccaaatgaa atattaagat ctgttataat
     5701 atctggaata gcagggatta acatagtttc tgcatacatt accatgatag cgattatacc
     5761 aagcaaagac agagttttcc aagcatatgg agatattttt gtgtctattt ctttttgttg
     5821 gtccttatta tctttttcct taatattttt caagctggtg ttgttgctcc taattgttga
     5881 tttaagtggg taaaaatttg ttactacaag aaattttagc aaaagcaagt ttttttaggt
     5941 gaataatttt tgagttttct ttttgtagaa tcaataattc aatagaattt tttatatttg
     6001 cttttagaaa tattaaatat ctcatagtat ataaaaaaaa tctcatggtg ggatagcctc
     6061 ttttataaat aataatatta aaatttactt tacaaaaaaa agttatggtt atacttttat
     6121 ttattcttta gcagatttaa atattttttc taattcacta tagaactctt tgtttacagc
     6181 agactttgac agagatgctt gccattgttt tgctactgtc tttatagtta gatctactgc
     6241 cttgtcaatt gtactccccc attctctttc aatctgcttt ttcaatttct ccacatgtac
     6301 ttccctaagt gcagtaaaga atgatctttc caaaagtgat tttgctgcag caataggatc
     6361 atcactacaa cattgaccac tattactatt tgttttattt gcagtagtgg tttgacaata
     6421 acaacattca tttgcatcat tatgatgtcc tgaacttctt tgtgtttcca cacattcaca
     6481 atttgattca cattgatttt cttccatatt tattacctct tgttatctac tatagttagg
     6541 taagtatata aattagtaag tgaaactgaa gttagtaggt gaaaggtggg taagttgact
     6601 gaacaaaagc tactagatga agacaacaac agggaaacta gtcaatttat gttagatggt
     6661 atgaattgca aatgttgccc agtttttaat acacttagta taatcggaaa gaaatttacc
     6721 cttttattat tacgcaatat gattttccta aaacaaaaac gatttaatga atttcttaat
     6781 tcaatagagg agataaatcc aaaaactttg tctatacgtc ttaaagaatt agagagagac
     6841 agattaataa agcgagaagt atttaatgaa actccggtta ggatagaata ctaccttaca
     6901 gaaaagggaa aggcattaca gccaatatt
//
LOCUS       DASVEV010000168        20047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_646715, whole genome shotgun sequence.
ACCESSION   DASVEV010000168 DASVEV010000000
VERSION     DASVEV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 20047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20047
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646715"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1667)
                     /gene="cysS"
                     /locus_tag="VF222_07585"
     CDS             complement(279..1667)
                     /gene="cysS"
                     /locus_tag="VF222_07585"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7142323.1"
                     /translation="MKLYNTLSTKVDELQIYDNSVKIYLCGITVYDESHIGHARTIII
                     FDVLHRYLLSKQHKVVFIQNFTDIDDKIIKKAKSLNKDYKEVTQKYIKQYLEDASRLN
                     VLEEIHYPKATDHISNMIELIKCLLEKNYAYITLNGVYFEVKKFSHYGKLSKKTSESI
                     ESGARIEIDPTKKDPLDFALWKFSSESPLWESPWGAGRPGWHIECSAMALKHLGNTMD
                     IHGGGNDLIFPHHENEIAQSESMTDKEFAKIWMHCGMVNLNSEKMSKSIGNIISIRNA
                     INKWGANTIRILCLTTQYSKPLDYTLSHLIESQSKWRMIENCFYELQFPIVIEEETFD
                     AVKLSQNALSSIRNYLENDFNTPLALSEFLKFVSELNNYAAMEKLTIKTSNIVLPIFR
                     EVLYIFGLRVLELSDKDRVEINDHILQRNKFRKNKEFEKSDRIRIMLKENYGIELIDH
                     QNFRTIWKKVEK"
     gene            complement(1729..2034)
                     /locus_tag="VF222_07590"
     CDS             complement(1729..2034)
                     /locus_tag="VF222_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7142324.1"
                     /translation="MVWIKIAETGDIPNNKGKEINVNGNRVAIFHANGKYYALEALCR
                     HQDGSLAPGKIDGEVVECPLHFWHYNIKTGELLDYLKDVKLETYKLDIRENDIFIDI"
     gene            2205..3482
                     /locus_tag="VF222_07595"
     CDS             2205..3482
                     /locus_tag="VF222_07595"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7142325.1"
                     /translation="MKIQSSSPKKSSRVKYTNSRDLFESGKLAYEWTVGLMKIITKIT
                     DKYQRTKPLANFRLGFCLHITKETSVLLMAAKALGAQISICSANPLSIQAEVIEFLKY
                     NEIKVFAKEDSSREEYFENIIKVADTNPDVITDDGAELHSTVHQRKIRGIFGGTEETT
                     SGIFRILALQSKTGLNYPIIGVNNAKSKHLFDNRYGTGQSTIYGLLRALGILIAGKHF
                     VVCGYGWVGRGVSSCLRGLGAIVTITEVNPIKALEAHLDGFNVQRLRDILPKGDCFIT
                     CTGQRDVITKYNFDIIKNGAFLVNAGHFDVEIDVDYLYSQNKHPIELRPYVESFNIKG
                     KKINLIAKGRVINLVGGEGNSPEIMDLSFSNQLLSILYITKNYKNLGKELLQVPTIID
                     DHVAHAALSSFGIKIDKLSREQVDYHTKAYLHD"
     gene            3862..5595
                     /gene="metG"
                     /locus_tag="VF222_07600"
     CDS             3862..5595
                     /gene="metG"
                     /locus_tag="VF222_07600"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7142326.1"
                     /translation="MLSNSNNSNFYPINGLSDYSIKSEEANQPIVITTALPYANGEIH
                     LGHISSTYLPADIFTRFLRQSKKNVFHVCASDDFGTPILIKAEKENKTPKEFVDYWNK
                     RDLEDFNAVGISFDKFYKTSSNENQEFVQYVFNKLRSNGHIYEKEVIQFYCDFDKKFL
                     PDRYVIGICPYCKSKDQYSDLCEKCGRVPEEILDPKCGICGKSPIKKTSNHYFFKLSN
                     FSSELKQWLTANNNLQANIKNYVLHWIDEGLQDWDITRDLDWGINIPLHDAKGKVFYG
                     WFDNHLCYISTFNALMKERFGRQGKNIWNNASIYHFIGKDIIYHHFLFLPAIRMGINR
                     EYKLPDYIPVRGHLMLHNRKISKSRNWYISLKEFISTFNPDFLRFYIATISPSNQDDI
                     NFDWDVFSSKINNELIDNIGNFINRTLSFTKKNFDGIVPEPSIYHDDDKKGLEAIKNI
                     SKDTGELLEKTETDKALKTILKFSNFFNQYFQKKEPWSNKTSSATTLYLSINAVRVLA
                     ILLLPFIPNSAEKIWSQLNINNKLHEESWDGLSILKIQPGHRIGKISPLFKKIERKEI
                     EKEKTKLGTIS"
     gene            5592..6152
                     /locus_tag="VF222_07605"
     CDS             5592..6152
                     /locus_tag="VF222_07605"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HEX7142327.1"
                     /translation="MRFLSNEMIQYKRGLVIGRFQPFHNGHLELFQQILKECEELVVV
                     IGSAQFNYILKDPFTAGERIEMIHASLSFQRMDRSRFFIIPLSNFENNACWFDYLKSM
                     VPKFDILYSGNEYVRYLAEKEIIVKEHTFKNKFRFNGENIRRLIVNDEKWDDLVPIPV
                     REIIMNIGGVERIRILAKSDTHPQKW"
     gene            6160..6291
                     /locus_tag="VF222_07610"
     CDS             6160..6291
                     /locus_tag="VF222_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142328.1"
                     /translation="MEKKRECPICSKCGFNKFQVIENNTTDEFVKIQCLKCFNIALI"
     gene            7121..8119
                     /gene="ilvC"
                     /locus_tag="VF222_07615"
     CDS             7121..8119
                     /gene="ilvC"
                     /locus_tag="VF222_07615"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7142329.1"
                     /translation="MVKKWNDCDITFESIKNQMIAVIGYGIQGKAQANNLKDSGLNVV
                     IGLRKDGNSWRIAEADGQKVMEVRDAARIADIIHLLIPDMEQADTYKKEISPYVTENK
                     ALCFSHGASIHWRWIEPPTNVDVIMVAPKGPGQRVRELFQEGFGTPSLVAVYQDHTGT
                     ALNRVLALAKGIGSTRPGVIETNFKEEVETDWFGEQVDLCGGAHSLIMKAFETLVEAG
                     YQPEVAYFECLHEMKLIVDLIQKYGLTGMYTRVSETARFGGLTRGPRVIDETTKSKMR
                     EVLSEIQTGQFAHEWITSYKNEGKNAFQRYMHDIESHQIEKVGKEMRKMMWPEENS"
     gene            8244..9338
                     /locus_tag="VF222_07620"
     CDS             8244..9338
                     /locus_tag="VF222_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX7142330.1"
                     /translation="MVEISAIIGIVFTIIIIGYIAYQIYKRRYGVGQKSPFSGFGRQY
                     SRGGVTPEGEPLESYSSSGYNKSLFRVIIPAVIGLIIVIVLVTASVKTVEAGHRGVLL
                     HFGAVDTTASLDEGLHFVVPFRDQVIPIEVRTQKVVVSSTSASKDLQEVSTEVALNFH
                     IRPEAAQLLYRQLGFDFDNRVILPAIQESVKQVTARFNAEELITKRETVKNQIEQQIQ
                     ARLANYNIVLETLSITEFRFSVQFTKAIEAKVEAQQRALQAQNELLRIKIEANQTEAK
                     AIGDQRAAIAQANGLKQANVLKAEGESSAIKIIDEQLKSSPSYLEWLKTQRWDGKLPL
                     VTGGGGGAGGQGLTPFIEIPTSRNEQNSTS"
     gene            complement(9508..9936)
                     /locus_tag="VF222_07625"
     CDS             complement(9508..9936)
                     /locus_tag="VF222_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX7142331.1"
                     /translation="MTFSQSQEVKRTRVLVAKLGLDGHDRGALVICRALRDAGMEVIY
                     SGLFCTPEQVAKTAIDEDVDVIAMSLLNGAHLTLFPKVAKLLKEQGIDDILIIGGGVI
                     PGKDKMILENEGVTGNFGPGTPLQVIINHINSNIKKKKDI"
     gene            10122..11093
                     /gene="meaB"
                     /locus_tag="VF222_07630"
     CDS             10122..11093
                     /gene="meaB"
                     /locus_tag="VF222_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7142332.1"
                     /translation="MLKIVEGILKGDRRSLSRAISIIDNEEGKYQEIISQIFKNTGKA
                     MTIGLTGSGGAGKSSLIDKLIDEFKRFGYKIAIIAVDPTSPVSGGAILGDRVRMQANL
                     MEKSEVFMRSIASRGAIGGISRSLRNIIRILDAAGYNLIIVESVGAGQLEIEVSKVVN
                     LTLVVFTPNTGDKIQAVKAGITEIGDIYVVNKSDLEGATTLYNTIKDLIGETDRKPII
                     LKCSAKTGSGTRLVAETISKMLNERVDNFRKKDHKLLEIELKDMVLNILNDKIVRMLN
                     DNPSYHNLIDKIQNKKMDPFQAAEELTTNLLNYTISNNRENNKIIRR"
     gene            11100..12689
                     /locus_tag="VF222_07635"
     CDS             11100..12689
                     /locus_tag="VF222_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7142333.1"
                     /translation="MSKKSEIKTDSGFPVQEVYNRKDISKRIVKEEKPGKYPFTRGIH
                     DSMYRERLWTMRQYSGFGSAEETNARFKFLLDHGQTGLSLAFDLPTQTGRDSDNQQSE
                     GEVGRTGVAISSIEDMMACFDTIPLDKVSTSMTINSTAATLLSLYISVAESQGVKPAS
                     LRGTTQNDIMKEYIARNTYIYPPKPSLRLIGDMIQYCSKHVPQWYPISISGYHIREAG
                     SNATQELAFTFANAIEYIQTCINRGLKIDNFAPRLSFFFCCTMEFFEEIAKFRAARRI
                     FAKIMRERFHAKDSKSVTLRFHVQTSGESLTAQQVDNNIVRVTTEALAAVLGGCQSLH
                     TNSRDEALALPTEESVKIALRTQQMIATETGVTKTVDPLGGSYYVEYLTNKIEDEVEK
                     YLKRIDRMGGALSAVEKGFFQDEIRQNAYSLKKEIDEQKKIIVGVNKYQDQHDVEPLL
                     NVMDPEFEKKQVARLKELKMKRDKNKVDHAIELLVKNSEKENQNLMPHIINAVKTKVT
                     LGEISDAFQSVFGRYEPRISF"
     gene            13033..14055
                     /locus_tag="VF222_07640"
     CDS             13033..14055
                     /locus_tag="VF222_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX7142334.1"
                     /translation="MTKYNISLIPGDGIGPELMKSAKMLLESINDKTSIKFSLNEVDA
                     GDYALSKFGKALPDFTLDTIKKSDACLKAPVGESAADVIIVLRRLFDLYANIRPAKSL
                     PNIKSISEKVDLVTVRENTEDVYLGWEFYANSDTVVALRVISEKASRRIAEYAFKTAV
                     IRGDKKKVVAVHKANVLKKSDGLFKNVCREVSTKFPQIEYSELYVDACAMNLIRNPEN
                     FDVIVTTNLYGDILSDEAAQVVGGLGMGPSANIGENFSLFEPVHGAAFDIAGKNIANP
                     SSIFLTTKMMLEWLGAKFKDPLLFTEAVKIENAIVTLLKQNYKTIDIGGTLSTSEFTR
                     MVLDIL"
     gene            complement(14144..14545)
                     /gene="mce"
                     /locus_tag="VF222_07645"
     CDS             complement(14144..14545)
                     /gene="mce"
                     /locus_tag="VF222_07645"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7142335.1"
                     /translation="MRVDHIAIAVNNVEEALKNYRNILNVDKIHIEDIPNEKVRVVIL
                     TLEDTRIELLEPTADDSPISKFLKEKGEGIHHIAITADNIEQDVARGQKHGMRFLGGI
                     RTGSYGRKITFIHPKSLNGVLTEFCQSPPNN"
     gene            complement(15465..17006)
                     /locus_tag="VF222_07650"
     CDS             complement(15465..17006)
                     /locus_tag="VF222_07650"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7142336.1"
                     /translation="MDSNSDKKVKSNIYAGDQVRIFDTTLRDGEQTPGVSVNPEKKIK
                     IAFRLDELGVDAIEAGFPIVSIGERESIKTIAKQGLKADIYGLARTVQTDIDAALGCD
                     LEYIHTFIATSEIHMKYKLNMSKEQVLERAIWAVEYAKKHGLQVEFSAEDATRSDRDF
                     LIQVFKSVQEAGANRIDIPDTVGYATPDYISSLVNQVSENISLPISMHCHDDFGQAVA
                     NSLAGINAGAKCAHVTINGIGERAGNASLEEFVMSLHCLYNRKTNIQTELLYETSKFV
                     SNTMGIIVQPNKAIIGENAFGHESGIHTHGIINNPLTYEPINPELVGRKRWLQAGKHA
                     GAHGIKAMLNEFGVNPTEEELQKIVEQQKLLADKGKSITTAELLSISGQVMGNTKFEE
                     KFTLHDFHIVTGLNIIPTAVIQLSTQGKEYIASEIGVGPVDSALKAIQKIAVEVAKIK
                     VREYRLESITGGSDALAEVSVKVEDNFGNVVSARKSGADVVVASVQAMMDAINKVMLR
                     KVLES"
     gene            complement(17009..17536)
                     /gene="ilvN"
                     /locus_tag="VF222_07655"
     CDS             complement(17009..17536)
                     /gene="ilvN"
                     /locus_tag="VF222_07655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7142337.1"
                     /translation="MSSYDSDTDNTEQKTLPYRILSVIVDNKPGVLFRVTNLFRARNF
                     NIESITVGTTEQHDISRMTIAIQSDSRTTDQLVKQLRKLIDIIEVRILNTENTVFREL
                     ALIKMKAHDPTSRMEITHFSNIFRAKILDVSKDSMMVEITGTPDKIDAFKSIVDPYGI
                     IQIARTGISALPRGV"
     gene            complement(17547..19235)
                     /gene="ilvB"
                     /locus_tag="VF222_07660"
     CDS             complement(17547..19235)
                     /gene="ilvB"
                     /locus_tag="VF222_07660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX7142338.1"
                     /translation="MVEMIGAKALIASLEKEGVDIVFGLPGGANLPIYDELVDANFRH
                     ILVRHEQAAAHMADGYARIKRKAGVCIATSGPGATNLITGIATAHADSIPMVAITGQV
                     PVAMIGKDAFQETDIIGISNPCTKYAFQPRKAIEIPEVIKKAFYITESGRPGPVLVDI
                     PKDVQQEKAEIEFPELIKVRGYKPAFDPDLSQIEKAINLIMKAEKPIIMAGGGVILSG
                     AFHELQALAEILMCPVVTTFKGKGAFPENHPLAMGPIGMHGHAEANKIIIEADCIIAI
                     GARFSDRSVGRFDEFGKGMSIIHLDVDPAEIGKNKSVDVAVVGDVKSSLRTLVKMLTT
                     KGIRKDPENSWLKRRKELIEYYTSTIKDYSREITAKKTLKKLRELLPSEAIVTTEVGQ
                     CQMWASLHFDVITPGTFFSSTGLGTMGFGFPASIGAKAAKPDVPVIDIAGDGSFNMTE
                     NSLAVSVLEKLPVIVFLLNNYMLGMVAQWQRTFYNRRYMGVQQNRCPDYVKLAESYGA
                     QGIRVQSMNELDKAIKAGISSDVATVIDIPIDPEEDVYPFVAPGSGLKDMIVGG"
     gene            complement(19490..>20047)
                     /locus_tag="VF222_07665"
     CDS             complement(19490..>20047)
                     /locus_tag="VF222_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142339.1"
                     /translation="SADSQEEEATEGNVTIKGQPAITDTTEEPGMTTSSELQDEGQQQ
                     QQPLEGATGPQDEDQQQQQPLEGATDQTTKNESKMKVSEICNDEMDNDQDGIIDEEHE
                     CISQSSDTTATSEKIVPELATLDDNDKEEVEDEKNIQNEENSGKDPESRPSGVEILTT
                     GEICNDEMDNDLDGSIDEKKDCINK"
BASE COUNT         6891 a         3189 c         3223 g         6744 t
ORIGIN      
        1 attaatgttt caggaatgta aaatcaaaaa ttaaagaatt actgagaagt tctcaaaagt
       61 tgttctcaaa tatcgtgggt tattatatga gcggaatttt tgagaattta atatagagac
      121 aataataaat tatcttttga atatatctcc attaaagtat tatttattct ttaagaattc
      181 actagtactt tataattgta atatagaaac attagtatta tttcgatttt ttactttagt
      241 tatttaaatt ttttttaatt ttaattttat ctatgctgct atttttcaac ttttttccaa
      301 atagttctaa aattttgatg atcaattaat tctattccat aattttcttt taacattata
      361 cgaatacggt ctgatttttc aaattcttta ttttttctga atttatttct ctgaagaatg
      421 tggtcattta tttcaactct atctttatct gataattcta atactctaag tccaaaaata
      481 tacaaaactt ctctaaagat tggcaataca atattcgaag tttttatcgt tagcttttcc
      541 atcgctgcat agttattcaa ttcggaaaca aatttcaaaa attcagaaag tgcaagagga
      601 gtgttaaaat cattttctag atagtttctt atggaactta gagcgttctg tgataacttt
      661 acagcatcaa acgtttcttc ttcaataaca attggaaact gtaattcata aaaacaattc
      721 tcaatcatcc tccatttaga ctgagattca ataagatgcg agagggtata atctaatggt
      781 tttgaatact gtgtagttaa acaaagaatt cttatggtat ttgctcccca tttattgatt
      841 gcatttcgta tagatattat gtttcctatc gatttggaca tcttttcaga attaagattt
      901 accattccac aatgcatcca aatctttgca aattctttgt ctgtcataga ttcggattgt
      961 gctatttcgt tttcatgatg aggaaaaata agatcatttc cacccccatg aatatccatt
     1021 gtattaccaa gatgttttaa tgccattgca gaacattcta tatgccatcc tggacgtcct
     1081 gctccccagg ggctttccca tagtggcgat tccgatgaaa atttccataa ggcaaaatct
     1141 agcggatctt tttttgtagg gtctatttct attctcgctc ctgattcaat actttcggaa
     1201 gtttttttag atagttttcc ataatgagaa aattttttta cttcaaaata gacaccattt
     1261 aaagtaatat aagcataatt tttttcaagc aagcatttta tcaattcaat catattgctt
     1321 atatgatctg ttgcctttgg ataatgaatt tcttctaata cgtttaatct agatgcatct
     1381 tccaaatatt gtttaatata tttttgtgtt acttctttgt aatctttgtt aagtgacttg
     1441 gcttttttta ttattttgtc atcaatatcg gtaaaattct gaataaatac aactttatgt
     1501 tgttttgata aaagatatct atgtaaaacg tcaaatataa taatggtgcg cgcatgacca
     1561 atgtgagatt catcataaac agtgatccca cataaataga tcttcacact attatcatat
     1621 atttgtaatt catcaacttt ggtggataaa gtattatata gtttcaaacg caatcttttt
     1681 tatttaattg aactataaat acataataaa agaagaacgt acgactcatt aaatatcaat
     1741 aaatatatcg ttttcacgta tgtccagttt ataagtttcc aatttaacat ctttaagata
     1801 gtcaagtaat tctccagtct tgatattata gtgccagaaa tgaagaggac actctactac
     1861 ttcaccatca atttttcccg gtgcaagaga cccatcttga tgtcgacaca atgcttcaag
     1921 agcatagtac tttccatttg catgaaagat tgccactcga tttccgttta cgtttatttc
     1981 ttttccctta ttgttaggaa tgtcacctgt ctctgcaatt tttatccaaa ccatgaactt
     2041 acaatcttaa tttagtctcg gttataattt tcttaccact ctttattatg tttttaataa
     2101 tgaaatccca tctctaaatc aaaaattctt tcagaaagtg aatttcatta agccgactcg
     2161 taatatttca catttaatat ctatattatt ataattataa taaaatgaaa atccaatctt
     2221 catcaccaaa aaaatcttct agggtaaaat atactaattc tcgagatttg tttgaaagcg
     2281 gaaaattagc atacgaatgg acggtaggcc taatgaaaat tattacaaaa ataacggata
     2341 aatatcaaag aactaaacct ttagctaatt ttagattagg gttttgtttg catataacaa
     2401 aagaaacttc cgtactctta atggctgcga aagcacttgg agcgcaaatt tctatttgtt
     2461 cagcaaatcc tctatccatt caagccgaag taatagagtt tttaaaatac aatgagataa
     2521 aggtatttgc aaaagaagat tccagtagag aggaatattt tgaaaatatt attaaagttg
     2581 cagatacaaa ccctgatgtc attactgatg atggcgcaga attacacagt actgttcatc
     2641 aaagaaaaat aagaggcata tttggaggaa ccgaagaaac tacctcagga atttttagaa
     2701 ttttggcact tcaatccaaa actggattaa attatcctat aattggtgta aataatgcta
     2761 aatccaaaca tctttttgat aacagatatg gaacgggtca aagtacaatt tacgggttat
     2821 taagagcttt aggaatatta atcgctggga aacactttgt agtttgtggt tatggttggg
     2881 ttgggagagg tgtgtcatca tgtcttcgag gcttgggagc catagttacg ataaccgaag
     2941 tgaatccaat aaaagcatta gaagcacatc tggatgggtt taatgtacaa agacttcgag
     3001 acattttacc aaagggtgat tgtttcatta catgtactgg acaaagagat gtaataacaa
     3061 aatacaattt tgatatcata aaaaatggag catttcttgt taatgccggt cattttgatg
     3121 tagaaataga tgtagattat ttatacagtc aaaacaaaca tcctatagag ctgagaccat
     3181 acgtagaatc atttaatatt aaaggaaaga aaattaacct aattgcaaaa ggaagggtta
     3241 taaatttagt aggaggagag ggaaattcac cagaaataat ggatttatct ttttcaaatc
     3301 aattgttatc aatattgtat atcacaaaaa attataaaaa tttaggaaaa gaacttcttc
     3361 aggtacctac aataattgat gaccacgttg cacatgcggc tctatcctca tttggtataa
     3421 aaattgataa actttcaaga gagcaagttg actatcatac taaagcttat ttacatgatt
     3481 gaatataaca tttaattcta acaatccacc tcaatgagag tttttattct atttatctca
     3541 gcaaaactga agaaaaaatt agattgtctt caaagatgga aagcaacgat taaactcaaa
     3601 tgacacgtca aagtacacct atttaataaa tatataaaaa gtaaaaactt ttataaagtt
     3661 tttttagatg aaaacataaa tttttccatt aacaaagtgt aaccatttgc acaaaacgtt
     3721 gaaaactaat atgtaaatat ttaaaatata aagtattgtt tcttactgtt aattattaag
     3781 ttgatgtaat ttataaaatt aacaaatgta atagaaaccc aatatcattt tattacggaa
     3841 atttaaaatc cttatcatct aatgctctcc aacagtaata atagtaattt ttaccctatt
     3901 aatggattat ctgattatag tattaaaagt gaagaagcaa atcaaccaat agtaatcact
     3961 accgcacttc cctatgctaa tggcgaaatc caccttggac atatatcatc aacatatctc
     4021 ccagccgata tcttcactag gtttttgcga caatctaaaa aaaatgtatt tcatgtttgt
     4081 gcgtctgatg attttggtac acctatttta attaaagcag aaaaagagaa taaaactcct
     4141 aaggagtttg ttgattattg gaacaaacgc gacctagagg attttaatgc agttgggatt
     4201 tcatttgata aattttataa gacaagttct aatgaaaatc aggaatttgt acaatatgtt
     4261 ttcaataaat taaggagtaa tggtcatata tatgaaaaag aagtaattca attttattgt
     4321 gattttgata agaaattttt accggataga tatgtgatag ggatttgtcc ttattgtaag
     4381 tcaaaagacc aatattcaga tctatgtgag aaatgtggac gagttcctga agaaatattg
     4441 gatcccaagt gtgggatatg tggaaaatca ccaataaaga aaactagcaa tcattatttt
     4501 tttaaactat ctaacttttc ttctgaacta aaacaatggc tcaccgccaa taataatctt
     4561 caagcaaaca taaaaaacta cgttctacac tggattgacg aaggattaca ggattgggat
     4621 attactagag atcttgattg gggaataaac attccattac atgatgcaaa gggaaaagta
     4681 ttctatggct ggtttgataa ccacctatgc tatatatcca ccttcaatgc tctaatgaaa
     4741 gaaagatttg gtagacaggg aaaaaatatt tggaataatg catctatcta tcatttcatt
     4801 ggaaaagata taatatatca tcatttcctt tttcttcctg ccattagaat gggaataaat
     4861 agggaatata aactacctga ttacattcct gttagaggtc atttaatgct tcataataga
     4921 aaaatatcaa aaagccgaaa ttggtatatt agcctaaaag aatttatatc tacctttaac
     4981 ccagactttt tgagatttta tattgctaca ataagtcctt ctaaccaaga tgatattaat
     5041 tttgattggg atgttttctc gagcaaaatt aataatgaac ttatagataa tattggaaat
     5101 ttcattaaca ggacgttatc ttttactaaa aaaaattttg acggaattgt accagaacct
     5161 agcatttatc atgacgatga caaaaaggga ctagaggcaa taaagaatat atccaaagat
     5221 accggagaat tattagagaa gacagaaact gataaagcac taaaaaccat tctgaaattc
     5281 tctaattttt tcaatcaata ctttcaaaaa aaagagccat ggagtaacaa gacttcgtca
     5341 gctactactc tttatctttc aattaatgct gtcagagtcc ttgcaatact tttattacct
     5401 tttatcccta attctgctga aaaaatttgg tcacaattaa acattaataa caaattacac
     5461 gaagaatcat gggatggttt atctatatta aaaattcaac ctggtcatag aataggtaag
     5521 atctcaccat tatttaaaaa aattgagaga aaggaaattg aaaaagagaa aacaaaattg
     5581 ggaacaatta gttgagattc ttatcaaatg aaatgattca atataaaaga ggactagtta
     5641 ttggaagatt tcaacctttt cataatggcc atttagaact ctttcaacag atcctaaaag
     5701 aatgtgaaga attggtagta gttataggaa gcgcccaatt taattacata ttaaaagacc
     5761 cctttactgc aggagaaagg atagaaatga ttcatgcctc actttcattt caaaggatgg
     5821 accgtagtag gttttttata atccctttat caaattttga gaataatgcc tgctggtttg
     5881 actatttaaa atcaatggtt ccaaaatttg atattttata ttctggcaat gaatatgtac
     5941 gatatttagc agagaaagaa ataatcgtca aggaacatac ctttaaaaat aaattcagat
     6001 tcaatggaga aaatattcgt aggttaatag taaatgatga aaagtgggat gatttagttc
     6061 ctatacctgt cagagagata ataatgaata taggcggagt tgaacgaata agaatcctag
     6121 caaagtcaga cacccatcca caaaagtggt gaataatcta tggaaaagaa aagagaatgt
     6181 ccgatttgta gcaaatgtgg ttttaacaag ttccaagtta tagagaataa tacaactgat
     6241 gaatttgtca agattcaatg cttaaaatgc tttaatatag ccttgatata gaaatatatc
     6301 ttttttatca ttacaagtgt gttaatttga gcaacataat tcaaatcgct cctggtttaa
     6361 caaagatcat taaaggaaat cttaatttga acctttaaat aaatcttatt ttatgtttgt
     6421 attttataga gacacatata ctaaactatt tatattgtat aacctttagc tcctgtcaaa
     6481 tttagtattt taccaaataa taaagtattt taattctctt tcagagcata ctataaattc
     6541 aaagaaaaaa agctgaaact ttttacaaaa aattataatt atttatgtgt gtttcaataa
     6601 ttttatttat aatacttttt aagcattaca aagtcataac tatgtattat gggcctatca
     6661 atatatgcaa ttaaattaga acagttgtta agttctctta acttctaact tatatattta
     6721 caagatattt tcacatttga aaattcggat tttttacaat cacatgattt tccaaagccg
     6781 gtatcttcca atagtgaaga acaattaggt tactgcattg agatgttatg ctgaaaattt
     6841 taatccttaa ctaaacttca ggctttgaac acgatcatta tatttgaata aaccaacttt
     6901 gaaaataaca tgttaaaatg gtcagtatta atatatatat ttaaatacag tattcaaaaa
     6961 gcacgttcag gatttatacc tatattttat tttattaata tgatttttgt ctaccagtat
     7021 aaaaatgtaa aatatcaaat ttagagagag atttatattt tagtcaaact tacgttcatt
     7081 agcagttatt atttataaaa cctctattta atatcttttt atggttaaaa aatggaatga
     7141 ttgtgatata acatttgagt ctataaagaa tcaaatgatt gcagtaatcg gatatggaat
     7201 tcaaggaaaa gctcaagcta ataatcttaa agattctggt cttaatgtgg ttataggatt
     7261 acgaaaagat ggtaattcat ggagaattgc cgaagcagat ggacaaaaag ttatggaggt
     7321 aagagatgct gccagaattg ctgacataat tcatctatta attccagata tggaacaagc
     7381 agatacttat aaaaaggaaa ttagtccata tgtaactgaa aataaagcgc tttgtttttc
     7441 tcatggtgct tccattcact ggagatggat agagcctccc actaatgttg atgttattat
     7501 ggttgcacca aaaggaccgg gacagagagt tagagagctt tttcaagagg gctttggtac
     7561 accttctctg gttgcagtat atcaagatca tactggaact gcattaaata gggttcttgc
     7621 tttggcaaaa ggcataggaa gtactaggcc tggtgtaata gagacaaatt ttaaggaaga
     7681 agtggaaact gattggtttg gagagcaagt tgatctttgt ggaggtgctc attctctgat
     7741 aatgaaagca ttcgaaacct tagttgaagc tggatatcaa ccggaagtcg cctattttga
     7801 atgtctgcat gagatgaaat taatagtaga tctgattcaa aaatatggac taactggaat
     7861 gtatactagg gtgagtgaaa ctgctagatt tgggggatta acaaggggac cacgagtgat
     7921 agatgaaact accaaatcaa agatgagaga agtgttatct gaaatacaaa ctggacaatt
     7981 tgcacatgaa tggataacta gttataaaaa cgaaggcaaa aatgcatttc aaaggtacat
     8041 gcacgatata gaaagtcatc aaattgaaaa agtaggcaaa gaaatgcgca aaatgatgtg
     8101 gcccgaagaa aatagctaaa attcggattt atttttttat ggaattgtta aattatagct
     8161 tatgtctctt tcaacataga taatgatttg tattaattta taagtaaata ataataaata
     8221 atccttacta aggagaacaa ataatggttg aaattagtgc gattattgga attgtattta
     8281 caattataat cataggctat attgcctatc agatctacaa acgtcgatat ggtgtaggac
     8341 agaaaagccc atttagtggt tttggaagac agtactctcg tggaggagtc actcctgaag
     8401 gagaaccgtt agaatcatat tcctcttcgg gatacaacaa atcgttgttt agagtaataa
     8461 ttcctgctgt aattggattg attattgtaa tagttttagt aactgcaagt gtcaagaccg
     8521 ttgaagcagg acatagagga gttttattgc actttggtgc agttgatact actgccagtc
     8581 ttgacgaagg tcttcatttt gtagttccat ttcgagatca ggtaatacca atagaagtta
     8641 gaacacaaaa ggtggttgtt agttctacct cggcttcaaa agaccttcaa gaagtgagta
     8701 cagaagttgc tctaaatttt catatacgac ctgaggcagc ccaattattg tatagacagt
     8761 tgggatttga ctttgataat agggttatat tgcctgcaat acaggaatct gtgaagcagg
     8821 ttactgctcg attcaatgca gaagaactga ttaccaaaag agagacagtc aaaaatcaaa
     8881 ttgaacaaca aatacaagct agacttgcta actataatat tgtactggaa accttgtcaa
     8941 ttacagaatt cagattttca gttcagttca caaaagctat agaagctaaa gtggaagctc
     9001 aacagagagc tttgcaagca caaaatgaat tgctcagaat caaaattgaa gctaatcaaa
     9061 ccgaggccaa agcaatagga gatcaaagag cagcaatagc acaagctaat ggtcttaaac
     9121 aagcgaatgt attaaaagct gaaggtgaat catcagccat aaaaattatt gatgagcaac
     9181 tgaagagtag tccatcatat cttgaatggc ttaagacaca aagatgggat ggtaaactgc
     9241 ctttggttac aggaggtgga ggcggtgctg gtggacaggg tctaactccg ttcatagaaa
     9301 ttcctacctc tagaaatgaa caaaattcta caagttgatg acatcagccg ctagaaagaa
     9361 gtatattaaa gagaaatacc ctaatttttc tttattcttt aatatataat ttttattact
     9421 ctttaacttt tttaatagaa caaaataatt tcctatacct taaaaagtaa aataaatttt
     9481 gataactttt atttgttatg cctaaattta aatgtctttc tttttcttta tgttcgaatt
     9541 gatatggttg ataattactt gaagtggtgt tcctggacca aagttacctg tcactccttc
     9601 gttttctaga atcattttat cctttccagg aattacacct ccaccaataa tcaagatatc
     9661 gtcaatacct tgttctttta gtaattttgc cacctttgga aaaagtgtta aatgggcccc
     9721 attaagtaaa ctcatagcga taacatcaac atcctcatca attgcagttt ttgcaacctg
     9781 ttcgggagta caaaataatc cagaataaat tacttccata cctgcatctc ttaatgctct
     9841 acaaataacc aacgctcctc ggtcatggcc gtctaaaccc aatttagcaa ctaaaactct
     9901 ggtgcgtttg acttcctgac tctggctaaa ggtcatattc tacaagtaat tattagtaaa
     9961 tggggatttt taatctttat cttatttcac atttatttat aacctaaaat atatttaata
    10021 atgccattag tttcttctag tccgtaaaaa caataattta aattcttaat taaaatatta
    10081 tttataacta acaatatctt taaattttga gataaacatt tttgctaaaa atagttgaag
    10141 gaattttaaa aggagataga cgttcacttt caagagcaat ttctataatc gataatgaag
    10201 aaggaaagta tcaagaaatt attagccaaa tttttaaaaa tacaggaaaa gcaatgacta
    10261 ttggattgac aggctcagga ggcgctggaa agagttccct cattgataaa ttaatagacg
    10321 aatttaaaag atttggttat aaaatcgcca taattgctgt agatcctact agtccagtat
    10381 ctggtggagc gattttaggg gatcgtgtaa gaatgcaagc caacctaatg gaaaaatcgg
    10441 aagtgtttat gagaagtatt gcctctagag gtgctattgg aggaatttcc agatcattga
    10501 gaaatatcat tagaattctt gatgccgcgg gttataattt gataatagtc gagagtgtag
    10561 gcgctggtca attagaaata gaagtgtcta aagtggtaaa ccttactctt gtagttttta
    10621 ctcctaatac aggagataaa atacaggctg ttaaagcagg tattaccgaa attggggaca
    10681 tatatgtagt aaataaatca gatcttgagg gggctaccac tctttataat acaataaagg
    10741 atttaattgg agaaacagat agaaaaccca ttatactaaa atgttctgca aaaacagggt
    10801 cgggtactag actggtagca gaaacgatta gtaaaatgct caatgagcga gtagacaatt
    10861 ttaggaaaaa agaccataag ttattagaaa tagaattaaa agatatggtt ttaaatatcc
    10921 ttaatgataa aattgtaaga atgttgaatg ataatccttc ctatcataat cttattgata
    10981 aaatacaaaa caaaaaaatg gatccatttc aagcagcaga agagctgact actaatcttt
    11041 taaattatac aattagtaat aatagagaaa ataacaaaat tattaggaga tgaaataaaa
    11101 tgtctaaaaa atcagaaata aaaactgatt caggatttcc agttcaagaa gtttacaata
    11161 gaaaagatat ttcaaagaga attgtaaaag aagagaaacc cggcaaatat ccatttacaa
    11221 gaggaattca tgatagtatg taccgtgaac gcctatggac tatgagacag tatagcggtt
    11281 ttggaagtgc tgaggaaact aatgctcgat tcaagtttct tttagaccat ggtcaaacag
    11341 gcctatctct tgcttttgat ttaccaactc agactggaag ggattctgac aatcaacaat
    11401 ctgaaggtga agttggaaga accggagttg caataagttc aattgaagac atgatggcct
    11461 gttttgatac tattccctta gataaggtaa gtacatctat gacaatcaat tcgactgctg
    11521 ctacactctt gtcgttatat atatctgttg cagagtcaca aggtgtcaaa ccggcttctt
    11581 taagaggaac tactcaaaat gatataatga aggaatatat cgctaggaat acttatattt
    11641 acccaccaaa accatcactt cgtctgatag gtgacatgat ccaatattgt tcaaaacatg
    11701 tccctcagtg gtatcctata agtatatctg gatatcatat acgcgaagct gggtcaaacg
    11761 ctactcagga gttagcattt acttttgcaa atgcaattga atatatacag acatgcatta
    11821 atagaggact taaaattgac aatttcgccc cacgtctttc atttttcttc tgttgtacta
    11881 tggaattttt tgaagagata gctaaattta gagcagccag aagaatattt gctaaaataa
    11941 tgagagaaag atttcatgct aaagatagta aatcagtaac tttacgattt catgttcaga
    12001 ctagtggaga gtccttaaca gctcaacaag ttgacaataa cattgtacga gttacgacag
    12061 aagctcttgc ggccgtttta ggaggttgcc aatctttaca tacaaattct agggatgagg
    12121 cactagcatt accaactgaa gaatcagtaa aaatagcttt gagaacacaa caaatgatag
    12181 ctacagaaac aggagtaaca aaaactgtag atccattagg tggatcatat tatgtagaat
    12241 atttaactaa taaaatagag gatgaagttg aaaaatactt aaaaagaata gatagaatgg
    12301 gtggagcact ttctgctgta gaaaaaggat ttttccagga tgaaataagg cagaatgcat
    12361 actcattaaa aaaagaaata gacgaacaaa aaaaaatcat agtgggagtc aataaatacc
    12421 aagatcaaca tgatgtagag ccattactaa atgtcatgga tccagaattt gaaaaaaaac
    12481 aagtggcaag gttaaaggag ttgaagatga aaagagacaa aaataaagtt gatcacgcaa
    12541 tagaactttt ggttaagaac tctgaaaaag aaaatcaaaa tttaatgcct cacataataa
    12601 atgcagtaaa aactaaggtt acccttggtg aaataagtga cgcttttcag tcagtatttg
    12661 gaagatatga accgaggata tctttttaat tctaattaag ttgattatta ttttattgat
    12721 cttttaaatt ttaagaatga acaataataa tttttttctt tgtgttcatt tagattaata
    12781 caaaatagga ttagaattct tccctaaaat attttagtgt taatcataag aattaaattt
    12841 atttcataca atagttcgaa aaaaaattaa ataagaaaac tgttaaacga atcaacaaat
    12901 ctcaaattaa tttcctataa tatcctataa aaagtttttc tttatagtat gcttttatat
    12961 attaaaaaaa aggcgaccta atgttgttaa gggaacaatt tttaaaacaa ttagattatt
    13021 actttacatt acatgactaa atataatatt tcgttgattc ctggtgatgg aattggacca
    13081 gaattaatga aaagtgctaa aatgcttctt gaatctataa atgataagac atcaattaaa
    13141 ttcagtctta atgaagtaga tgcaggggat tacgcattat ccaaatttgg taaagcttta
    13201 cctgatttta cacttgacac aataaagaaa agtgacgcat gtttaaaagc tcctgtcggt
    13261 gaaagcgctg cggacgtaat tatagtatta agacgacttt ttgacctcta tgctaatata
    13321 aggcctgcta aatctttacc caatataaaa tccatttcgg aaaaggttga tttagttaca
    13381 gttcgagaaa atacagagga tgtctattta ggatgggaat tttatgctaa ttccgataca
    13441 gttgttgcac ttcgagttat atcagaaaag gcgtctagaa gaattgcaga atacgctttt
    13501 aaaacagcag taattcgggg tgacaaaaag aaggtagttg cagttcataa agccaacgtg
    13561 ttaaaaaaga gtgatggatt atttaagaat gtttgtagag aagtttccac aaaatttcct
    13621 caaatagaat atagtgagtt atatgtagat gcgtgtgcta tgaatttaat tagaaaccct
    13681 gagaattttg atgttattgt taccactaat ctttatggtg atattctttc tgatgaagca
    13741 gcgcaggtcg ttggaggatt aggaatggga ccatcagcta atataggtga aaacttttct
    13801 ttattcgaac ctgtacatgg tgctgctttt gatattgcag gtaaaaatat tgctaatcct
    13861 tcttctatat tccttactac aaagatgatg ttagagtggc taggagcaaa atttaaagat
    13921 cccttattat ttactgaggc agtcaaaata gagaacgcaa ttgttactct attaaagcaa
    13981 aattataaga cgatagacat tggaggcacc ctctctacta gtgaatttac tagaatggtg
    14041 ttggatatat tgtaattaat gattttgttt atagtcaggt cccttctatt atcatgttcg
    14101 attatttaat atattataac attgaatcag attcaatctg ttattagtta tttggaggcg
    14161 attgacaaaa ttctgttaat actccattta atgatttagg atgaatgaat gttatcttac
    14221 gaccatacga tccagttctt attcccccaa gaaacctcat accatgtttc tgtcctctag
    14281 cgacatcttg ttcaatattg tcagcagtta tggcaatatg atgtataccc tctccttttt
    14341 cttttaggaa tttagatata ggactatcat ctgctgtagg ttctaggagt tctatacgtg
    14401 tgtcttccaa agtgagaatt actacacgga ctttttcatt aggtatatct tcaatatgta
    14461 ttttatcaac attaagaata tttcgataat tctttagtgc ttcttcaaca ttattaactg
    14521 caatagctat atgatctact ctcatctttc ataaaagaag aatcacctat agatatatta
    14581 gttttctaat tggagatcac tgatagatgt aaactttgac aataaataaa tttgttcgaa
    14641 atgcagtgac tagtgaaaga aaaaaagaca caatactctc caggaactat atttatggta
    14701 atagaataac ataatattcg agttgccaat tatgatttga gagtttaaac cttctttgta
    14761 cccatgaaat ttaggtgtta tacacaaatt cgtcaagaat aatgagaatt atagatgtca
    14821 ttttattggc tgaggtcatg aatctatcta atgcctatga ataaacatat gttaaactaa
    14881 gaaaaaaatt gatttcatta gcccgttaaa attaagaatt tttgacaatt tagtatgtgt
    14941 taaaaaagtt aaattcaatt ataattggct atcttgattt gatggattaa taaaataaaa
    15001 tacgattaaa acataaaaaa aatttaatct ataaaatact aaaaaaaatc agaaatttta
    15061 ataaattctc cttgaatacc gaaattgctg ataacacgga ttattgtatt acataatttc
    15121 tcaaagatat tgctgctaat actgctcaaa catcatataa tattgaactt gtgttctaac
    15181 aataattatt caagtaaatt ttcaacgttt tccttcattg tatatgtgaa tagagaaagt
    15241 aaaataaccg gattatgata ccaaaataaa gaacttaaat ttcctagtat ggattatatt
    15301 ttttaaaata caatggaaaa taaaattgct tttatcattt atttaataag aaatttaatc
    15361 aataaacatg cataataaaa aataatatat atcataattg actttgaaca agttataaga
    15421 tttgaggatc aaggtaaaaa aaatgctctt ttataaaaac taatttagga ttctaaaact
    15481 tttctcagca ttaccttgtt gattgcatcc atcattgctt gaacagaagc aacgacaaca
    15541 tctgcccccg attttctagc tgaaactaca tttccaaaat tgtcttcaac tttgactgac
    15601 acctctgcta aagcatctga gccgcctgta atggattcca gtctatattc tcttactttt
    15661 atttttgcca cttcaacagc tattttctgg atcgctttga gagcagagtc tactggccca
    15721 actcctattt ctgatgcaat atattcctta ccttgagtac ttaattgaat tactgcagtt
    15781 ggtataatgt tcaaccctgt aacaatatga aaatcatgta atgtaaattt ttcttcaaat
    15841 tttgtatttc ccattacttg tcctgaaatt gataacaatt cagcagtagt aattgatttt
    15901 cctttatctg caagaagttt ttgctgttca acaatttttt gcaattcttc ctcagtagga
    15961 tttacaccaa attcgtttaa cattgctttg ataccatgag ctcctgcatg tttgccagct
    16021 tgtaaccatc tttttcttcc aacgagttca ggattaatgg gttcataagt taaaggatta
    16081 ttaataattc catgagtatg aattcctgat tcatgcccaa atgcattttc accgataatt
    16141 gctttatttg gttgaacaat tattcccatt gtattggaaa caaatttaga tgtttcatat
    16201 agtagttctg tttgtatatt cgtttttcta ttatagagac aatgtaatga cattacaaat
    16261 tcttcaagtg atgcatttcc agctctctct ccgatgccat ttatagttac atgggcacac
    16321 tttgctcctg catttatccc cgcaagtgaa ttagccacag cctgtccaaa atcatcatga
    16381 caatgcatac ttattggtaa tgatatattc tcagagactt ggtttacaag actagatata
    16441 taatcaggag tagcgtaacc aacggtatct ggaatgtcaa tcctgttcgc tccagcttct
    16501 tgaaccgatt taaaaacttg aatcaaaaaa tccctatcag aacgagtagc atcctctgca
    16561 gagaattcca cctggagacc atgtttttta gcgtactcaa ctgcccaaat agcacgttca
    16621 aggacttgct ctttactcat atttaattta tatttcatat gaatctctga ggtagcaata
    16681 aaagtatgga tatattctaa atcgcaccct aatgctgcat ctatatctgt ttgtactgtt
    16741 cgagcaagtc cataaatatc agcctttaag ccttgtttag caattgtctt tattgattcc
    16801 ctttcaccaa ttgaaacaat aggaaaacca gcctcaattg catcaactcc caattcatct
    16861 agcctaaacg ctattttaat ttttttttca gggtttactg agacaccggg cgtctgttcc
    16921 ccatctctta aggttgtatc aaaaatcctc acttggtcgc ccgcataaat attactttta
    16981 acttttttgt cactattact atccatcttt aaactcctcg tggtaaggca gaaattccgg
    17041 ttcttgcaat ttgtataatt ccatatggat ctacaatact cttaaaagca tcgatcttgt
    17101 caggagttcc tgttatctct accatcatag agtctttact gacatctaga atttttgctc
    17161 tgaatatatt tgagaaatga gtaatttcca ttctggatgt tgggtcatgt gccttcattt
    17221 ttatgagtgc aagttctctg aatactgtat tttcggtatt aagtatcctt acttcaataa
    17281 tatcaattaa ttttctaagt tgttttacca actgatctgt tgttcgagaa tcactctgga
    17341 tggcaatagt cattcgtgat atatcatgct gttctgtcgt gccaactgta atactttcaa
    17401 tattaaaatt tcttgcccga aataaattag taaccctgaa tagaacccct ggcttattgt
    17461 ctacgataac agataatatt ctatatggta atgttttctg ttcagtatta tctgtatcag
    17521 aatcgtatga tgacatattt ctttttttag ccccctacaa tcatatcttt taatcctgag
    17581 ccaggggcaa caaaaggata aacatcttct tctggatcta tcggaatatc tattactgtg
    17641 gccacatctg aacttatccc agctttaatt gctttatcta attcattcat agattgaact
    17701 cgtatacctt gtgcaccata tgactcggcc aatttaacat aatccggaca acgattttgt
    17761 tgaacgccca tatatcttcg gttgtaaaaa gttctttgcc actgagcaac cattcctaac
    17821 atgtagttgt taagtaaaaa tactatgact ggaagtttct ctaatactga tactgctaga
    17881 gaattttcgg tcatattaaa agatccatct cccgctatgt caattactgg tacatcaggt
    17941 tttgctgctt ttgctcctat cgaagcagga aatccaaacc ccattgttcc aaggcctgtt
    18001 gaactaaaaa aagtgcctgg agttataacg tcaaaatgca aagaagccca catttgacac
    18061 tgacctactt cagtagttac tatcgcttca gaaggtaata attctcgtaa ttttttaagt
    18121 gtcttttttg cagttatttc cctagagtaa tcttttattg tacttgtata atattcaatt
    18181 agctcctttc tcctcttgag ccaagaattt tctggatctt ttctaattcc ctttgtggtg
    18241 agcatcttta cgagtgttct taaagaggat ttaacatcac ctactacagc aacatccaca
    18301 gatttattct taccaatttc tgcaggatca acgtctaaat gaataatact cataccttta
    18361 ccaaattcat caaatcttcc aaccgatcgg tcagaaaatc tagcaccaat tgcaatgata
    18421 caatctgctt ctattattat tttatttgct tcagcatgtc cgtgcatccc aattgggccc
    18481 atagcgaggg ggtgattttc agggaaagcg ccctttcctt taaatgtagt cacaactgga
    18541 cacattaata tttctgccaa tgcttgtaat tcatgaaaag cccctgaaag aattacacct
    18601 cctccagcca ttataattgg tttttctgcc ttcataatta gattaatagc tttttcaatt
    18661 tgagacaaat ccggatcaaa agcaggttta tatcctctaa cctttatcag ctctggaaat
    18721 tctatttctg ccttctcttg ttgtacgtct tttggaatat ctaccaaaac agggccggga
    18781 cgaccactct cagtaatata aaatgctttt tttattactt ctggaatctc aattgctttt
    18841 cttggttgaa acgcatattt cgtacacgga ttagatatac caataatgtc agtttcttgg
    18901 aaagcatctt ttccaatcat tgccactggt acttggccgg taattgccac cattggtatc
    18961 gaatctgcat gagctgtagc tataccagtt ataagattag tagctccagg accagatgtc
    19021 gcaatgcata cccctgcctt tcgtttaatg cgtgcgtaac catccgccat gtgtgcggct
    19081 gcctgttcat gtcttaccaa gatatgtcta aaatttgcat ctaccaattc gtcataaata
    19141 ggcaagttag caccaccagg taatccaaaa acaatatcca ctccctcttt ttctaaagaa
    19201 gcaatcaatg cctttgctcc aatcatttca accataattt tttctccatt tttcttataa
    19261 tataagttgg ataaaactta atctaaagta ctaccacgat tctggctatc caaaagatag
    19321 ttctgaaatg tactttagat tccaactata tgaatataaa aagatactct atttatatag
    19381 ctactcaaaa atctatcaaa ataacagggt actgcaaaat ctgtctaaca tattcagaat
    19441 tttctagtta ttttcaagcc aaaaggtaaa gtttgaaaaa aataccattt tatttattaa
    19501 tacaatcttt cttctcatct atcgatccat ctaaatcatt atccatctcg tcattacaaa
    19561 tctctccagt agtaagtatt tcaactccac taggtcggct ttcaggatcc ttgccagaat
    19621 tttcttcatt ttgtatattt ttttcgtctt ctacttcttc cttatcgtta tcatctaaag
    19681 ttgctaattc tggaactatt ttttcactag ttgctgtggt atcggatgat tgggaaatac
    19741 actcatgttc ctcatctata attccgtcct gatcattatc catttcatca ttacagattt
    19801 ccgagacctt cattttgctt tcattttttg tagtttgatc tgttgctcct tctaatggtt
    19861 gttgctgttg ttgatcttca tcttgtggcc cagttgctcc ttctaatggt tgttgctgtt
    19921 gttgaccttc atcttgtaat tcagaggatg ttgtcatgcc tggttcttct gtcgtatctg
    19981 ttattgctgg ctgtcctttt attgtaacat ttccttctgt tgcctcttct tcttgagaat
    20041 ctgctga
//
LOCUS       DASVEV010000169        19611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_260725, whole genome shotgun sequence.
ACCESSION   DASVEV010000169 DASVEV010000000
VERSION     DASVEV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 19611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19611
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260725"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF222_07670"
     CDS             <1..1030
                     /locus_tag="VF222_07670"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142340.1"
                     /translation="EDSDNNNNNSYSFFSNLDKVFWDKTTNHPQLTKKNLIEYYDKIS
                     KYILPYLEDRPLSLSRYPDGIKGKSFYHKNWNQQNKPSFVQTVEVYSESRVDNVINYI
                     ISNNKETILWLANLGCIEMHPWYSRINNFEACKERDDILDKEKCSLNFPDFIVFDLDP
                     YIYSGQEKNKGQKEPEYNIKAFKATIDVAFNLKELFDDLKIESYIKTSGKTGLHIFVP
                     IANLYTYEQTRSFAEVIGKILINRHPRKITMEWDTTKRTDKVFFDHNQNARGKTIASI
                     FSPRPTNSASVSFPIKWNDLSHVVPTDFTIFNVPNTINKKIKNPWINIMEKKQDLNKI
                     LESIKEVL"
     gene            complement(1560..1841)
                     /locus_tag="VF222_07675"
     CDS             complement(1560..1841)
                     /locus_tag="VF222_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142341.1"
                     /translation="MVRYRNYSRSGNGLLILLAYAIITFVIQFVILLMRLLAYAIITF
                     VIPFLINMIKKKLGDQQRLLCNKCHGKLEVIDKGNFYCKNCKIIKMDRH"
     gene            1966..2298
                     /locus_tag="VF222_07680"
     CDS             1966..2298
                     /locus_tag="VF222_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142342.1"
                     /translation="MSFDVNSKIRAMIEVAANQQKLMLEAATNQFKFNLEGICNQQRI
                     FIETNSSMFKTVNPDINSAVEKAKTDLLQINKNVENEILKTIENAKNSIIQTIENSKK
                     NLLNTLSS"
     gene            2472..2873
                     /locus_tag="VF222_07685"
     CDS             2472..2873
                     /locus_tag="VF222_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142343.1"
                     /translation="MSHDNEILSDFTENVLNSEKYIRWVGITDQNGIIINEKHREGLK
                     LLLNNEENQESAVNTITRHKSRTKFEPKIGKLTYAFGRYENFSRFIIPISENYYLILT
                     IEFKEKHFDKIIIEKILPLIKEMKEKFRRLN"
     gene            3301..3891
                     /locus_tag="VF222_07690"
     CDS             3301..3891
                     /locus_tag="VF222_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142344.1"
                     /translation="MLIKKNAYSIISISITMTLILGVISSNDEDFSALSQQLPTDQTQ
                     LQDLISKSLDYVHQGDISTTSTNILLNGINLTQGEFILLYDSTPFGSKGHIAVNLPCN
                     PDNPHDPIFDILVGRAPDLFVLPLGYLEQISKPGKMCVYHGQFGFGDPITDLILKYVG
                     DDPIKLEGPHSIVISAHEFYNPTTESYFERSHNATN"
     gene            4739..5149
                     /locus_tag="VF222_07695"
     CDS             4739..5149
                     /locus_tag="VF222_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142345.1"
                     /translation="MINYIVLLSLVFLTISFFIYSIKAEIVPIFAESPTVKIFPNCGA
                     LSDHKIFFTVTSFSPNGNVHWEFIDSKGNIDSYGYFETDEFGGFEEYIIAEVVKPDIY
                     TLRFFDDKNNNFLKDFNGSEVIINYQIPCANPIF"
     gene            5408..5587
                     /locus_tag="VF222_07700"
     CDS             5408..5587
                     /locus_tag="VF222_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142346.1"
                     /translation="MSSSEENSNQNSSEELESAQKNQKKVGSAESDPSTTGPAENLRE
                     EAAQNTGEDDSKEPA"
     gene            5934..6299
                     /locus_tag="VF222_07705"
     CDS             5934..6299
                     /locus_tag="VF222_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX7142347.1"
                     /translation="MIKTKCLYYPKDISDGERILITRYWPRGIKKEHFDRWDKELSPS
                     RDLLKKYKNNKIDFQTFERLFLEEIEKSNAIDICKEIVNKNMKGKNITLLCYEKDEEI
                     CHRKFVKNICEQELLKINN"
     gene            6969..7391
                     /locus_tag="VF222_07710"
     CDS             6969..7391
                     /locus_tag="VF222_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142348.1"
                     /translation="MFLDIFIIARVFNILYTWLEHNIIDHILNFSKVFGNMIVLISVV
                     LFLLILAYITIKHLKKLPEFYSIDITDIDGNNVVIEGIRTKFTTYDAAKSYSEFYHDI
                     YKKQYKFRVVGSNQRYTLGFLDVVSLPKRASKAKEINS"
     gene            complement(8565..8792)
                     /locus_tag="VF222_07715"
     CDS             complement(8565..8792)
                     /locus_tag="VF222_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142349.1"
                     /translation="MSTDSPMDEISNAKTKVKDLENEIKNIDKGLELIKDTSGYKSNT
                     DLVKVYDNAITNYKNLVMAYKIYIETFEKNM"
     gene            complement(9651..10340)
                     /locus_tag="VF222_07720"
     CDS             complement(9651..10340)
                     /locus_tag="VF222_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7142350.1"
                     /translation="MPFGFSSSSKEDKDKEYSHHIKKSITDKHDVKEVNKINEMLNPD
                     EKVLLVAKQSKIKPGGSFFTPNTIYATDRRIIIRDPYMLGIKANVVDIPYDIITSLKL
                     EKGLLSSTIRFKAPGLMSSTKLGMMDSIIEGEDDQTGIIEAIPKDKAEDLLEIIRSGS
                     QDGRKSAPSKKHIHSKFLEPKDHTISSNPSVSIADELQKIAKLKEEGILTEIEFNQMK
                     QDLIRNIKKVG"
     gene            complement(10631..10993)
                     /locus_tag="VF222_07725"
     CDS             complement(10631..10993)
                     /locus_tag="VF222_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142351.1"
                     /translation="MVDDEKDVLDLFSDYLKSNGYNIISFDNPVKALEYFYKNTNNCS
                     VVITDYRMPQMSGLDFLKKIREKDTDHKIKTIIVSAYTKTDIPYEISYIMKIDKILEK
                     PVFLDELKTEIQKLIVSY"
     gene            12090..12314
                     /locus_tag="VF222_07730"
     CDS             12090..12314
                     /locus_tag="VF222_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142352.1"
                     /translation="MTSKFIQAALALSFSRVEEIKNNRIKELLEMKSKIINDPLVVEN
                     WFDNEINKIINVTGTSSHSTDLESIINKIK"
     gene            13335..13946
                     /locus_tag="VF222_07735"
     CDS             13335..13946
                     /locus_tag="VF222_07735"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEX7142353.1"
                     /translation="MNNNFIIVSLLVIFTVCITLSSIIGLNVEGQRENFNVINTDKSN
                     ISNIENFETYENSKYGIKIEYPSNWLKTEPGSTIGIDPYKQINVVTFSSPPNNDPVLM
                     VIVANKTAGTLVDFASGGINQHRIIYPDFNLLKLGNVNLGDNPGYNFTFSASDANGKF
                     KAAQIWTIKDNKLYLLVNTASEHLYPLTWPTIQKMIESFEIVN"
     gene            complement(14487..14744)
                     /locus_tag="VF222_07740"
     CDS             complement(14487..14744)
                     /locus_tag="VF222_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142354.1"
                     /translation="MKNRSRTEIVGNILDSANGGATKTKIMYKAFLSYGQLKEYLSIL
                     IENNLIEFLDGTQTFKTTEKGLNYLKKHNEMEELLQTTFKE"
     gene            15405..16127
                     /locus_tag="VF222_07745"
     CDS             15405..16127
                     /locus_tag="VF222_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7142355.1"
                     /translation="MSNNEKGLPEKSPDKLKKIKDYGIPLGIMAMSLVMIYILWASFG
                     YIGPSFSSQVLSHQQETLREKYNLPSLEDEDSSDTSEVPPSLRKVVESNTTPTSTSQN
                     SQSKMTTQSSTNTTISESSGNVTSKSTKEEPSQTTTTAAAGLTLTILEGSSIQGNPDY
                     DPDELTVKKGETILVDNVDTMPHTVTNGESGSDPNSGKLFDTSIINGGDSADMDTSTI
                     DAGTYPYYCMVHPYMTGSLTIE"
     gene            complement(16722..17063)
                     /gene="trxA"
                     /locus_tag="VF222_07750"
     CDS             complement(16722..17063)
                     /gene="trxA"
                     /locus_tag="VF222_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7142356.1"
                     /translation="MTKELEKVIPLNATQWSEKVLNSDIPVLVDFWAEWCGPCRMVGP
                     IVEQLAQSLEGKVKVSKLNVDQNQEIAMKYNIQSIPSLVLFKNGNEVARIVGLSPKEK
                     YEKFVNNALNV"
     gene            complement(17198..17509)
                     /locus_tag="VF222_07755"
     CDS             complement(17198..17509)
                     /locus_tag="VF222_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142357.1"
                     /translation="MYNNLQYQLMFIYDENVNSIDYALKELDKDTSNIFIYTDIKKFN
                     KPITIIQGLKDKDFIRIILRELKKNIGTGGTYKNGIIILQGNHQEYVKKYLMAKDIMK
                     E"
     gene            complement(17529..17750)
                     /locus_tag="VF222_07760"
     CDS             complement(17529..17750)
                     /locus_tag="VF222_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7142358.1"
                     /translation="MGWIQRKVFPNETPIRIEYYPTKKGIALQQPIIDMMAAYSMQYC
                     SKEIFKDGNQENAKYSTIEKFQGIEVTMT"
     gene            complement(17977..18720)
                     /locus_tag="VF222_07765"
     CDS             complement(17977..18720)
                     /locus_tag="VF222_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142359.1"
                     /translation="MICKLKIQANKRCEKLNTMDNDNKSILLESLNKQKVYEDDFISI
                     NDLRQSDIDILEILFDNGDENTIATFTFNGLKVRSNLHQEILSRSLKRLKELGLVDKN
                     KSGYNLNKKGILIFSKLKRLLDEKRNNQTKYTQIIQLSVPFQIPNQQIVQNLSGKYFG
                     SLRWIGMIQNIRGYQLKWQDIEDFMEIVVHLSNNNIIIETNEDNRLNISKAFSYSTRI
                     IESIGNIGMKNNNLFIYPINNKDFNKITN"
     gene            complement(18841..>19611)
                     /locus_tag="VF222_07770"
     CDS             complement(18841..>19611)
                     /locus_tag="VF222_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7142360.1"
                     /translation="RTFNAKVIGTDKLTDLAVLKIEHDSNNSSNSNNNTNNSDNKAID
                     LIPSAELGDSDNLKVGQIVIAVGNPFGLTGGPTVTTGIISSLNRNIEFEDGVLELVQT
                     DAAINPGNSGGPLVNTNGEVIAINTAKIPYAHGIGFAVPVNTAKTILQQLIKNGKVNR
                     PWLGITTLKITSRIARYYRLPTNEGALVVKVEEYSPASDSGIRPGDIIEEIDEKRIED
                     ITELSSRIKNKNVNEQTVLSVNRYGRRFTISVTLENQP"
BASE COUNT         7118 a         2625 c         2475 g         7393 t
ORIGIN      
        1 agaggatagc gataataaca ataataacag ttattctttc ttctcaaatc tcgataaagt
       61 attctgggac aaaacaacaa atcatccaca acttacaaaa aaaaatttaa ttgaatatta
      121 cgataaaata agtaaatata ttctccctta tctcgaagat agaccgttat ccttaagtcg
      181 atatcctgat ggaattaaag gaaaatcatt ttatcacaaa aattggaatc aacaaaataa
      241 accatcattt gtacagacag tagaagttta ttctgaatca agagtagata acgttatcaa
      301 ttatattata tccaataaca aagaaactat tctatggctt gccaatctgg gttgcataga
      361 gatgcatcca tggtatagta gaataaataa ttttgaagcc tgcaaagaaa gagatgatat
      421 attagataag gaaaaatgta gtttgaattt ccctgatttt atagtttttg acttagatcc
      481 atacatatat tcaggacaag aaaaaaataa aggtcaaaaa gagccagaat acaatatcaa
      541 agcattcaaa gcaacaattg atgtagcttt taacctaaaa gaattatttg acgacctaaa
      601 gatagaatca tatataaaga catcagggaa aacagggtta catatttttg ttcctattgc
      661 taatttgtat acttatgaac aaactagatc ttttgcagaa gttataggta aaattcttat
      721 caatagacat ccaagaaaaa ttactatgga gtgggatacc actaaaagaa cagacaaagt
      781 attctttgat cataatcaaa atgctagagg aaagacaata gcgtcaatat tttcgccaag
      841 acctactaat tctgctagtg tatcgtttcc tattaaatgg aatgacctct cgcatgtagt
      901 tccaacagat tttacaattt tcaatgttcc taatacaatc aacaaaaaaa taaaaaatcc
      961 atggattaat attatggaaa aaaaacagga cttaaataaa attctggaaa gtattaaaga
     1021 agttctttga tggaaagtat gacaatttac taaggatgta tttttaaaat attatcagtt
     1081 ttttacctcc attttttaag atataatttt tttaaacccc ctatttgaaa agataatatt
     1141 attagaaaat agaaaattat attttttcta taaatcacac tgataaatca aaaccagata
     1201 tattatatac taatttagga gcaattttcg caaaaatctt agacagaatc ggcatgagaa
     1261 ataaagaaga tgaaaatgga atgagacgaa aaatcacttt actacctatt ttagtcaata
     1321 tcaaactgtt cccacgtctt gatctattct ttaatctatg catatcttaa ttgggttcat
     1381 tttggtgtaa caaaatgaac taggcaaaac aatagaatat gaagaaggta aattacaaga
     1441 taatggacga attaaactag aatttcctaa tgaaaattac aaggagaaag tcaatcaata
     1501 atcctagtta ttctgatgta tagatatcaa tagcactttt ttatccaaaa tgcgcataat
     1561 taatggcgat ccattttaat tattttgcaa tttttgcaat aaaaattacc tttatcaatt
     1621 acttctaatt ttccatgaca tttattacat aaaagacgtt gttgatcgcc tagtttcttt
     1681 tttatcatat taataagaaa tggaataaca aatgtaatta tggcataagc tagcaatcgc
     1741 attagtagaa tgacaaattg aataacaaat gtaattatgg cataagctaa taatattagt
     1801 aggccattac ctgatctaga gtaatttcta tatcttacca tatcaatata attttgtatt
     1861 agaaatagac tatattaacg aattttttat ttatagtcat taatccacat cgaatatact
     1921 aatttaaata ttagttaact attattttta taatatatat ataatatgtc attcgatgta
     1981 aactccaaaa taagagcaat gattgaggta gccgcaaacc aacaaaaact catgcttgag
     2041 gcagcaacaa accaatttaa atttaattta gaaggaatat gcaatcaaca acgaattttc
     2101 attgaaacaa attcaagtat gtttaagact gtaaatcctg atattaattc tgctgtcgaa
     2161 aaggcaaaaa cggatttatt acaaattaat aagaacgtag aaaatgaaat attgaaaacc
     2221 atagagaatg ctaagaatag tataatacaa accatagaaa actcaaaaaa gaatcttctt
     2281 aatactctat catcataatt ttttaaatta atgtcaatta tttttaaaat cttcatttta
     2341 aattgttagt tatagtttta taaaaaacaa catacctttt gatcaataga actaacttca
     2401 tcgagtaatt ataatataat aataaagtca gaggaaattt tttattagat ataatacttt
     2461 tttttataaa tatgtcgcat gataatgaaa ttctttccga tttcactgaa aatgtattaa
     2521 attcggaaaa gtacatacga tgggttggta ttaccgatca aaatggtatt attataaatg
     2581 aaaagcatcg tgaagggtta aaattacttc ttaataatga agaaaatcaa gaatcagcag
     2641 tcaatacaat aactcgacat aaatcaagaa ctaaatttga accaaaaata ggaaaattaa
     2701 cctacgcgtt tggtagatat gaaaacttta gtagatttat aattccgata agtgaaaatt
     2761 attatttaat tttaacaata gaatttaagg aaaaacattt tgataaaata attatagaaa
     2821 aaattcttcc tttaataaaa gaaatgaagg agaaatttag aaggttaaat taatcattat
     2881 tagaattatt cattaatcaa attcataagg gtaaaaacct ttcccaaatt cccaaataaa
     2941 gatatttgtc caaatatgtt tcttactgtt attcctgtta acaaatgtaa aacaaaactg
     3001 atatatgtgt aactggtcta gtattattta atgatgatag gcaattgcga gtatgtgtta
     3061 ggcgagctgc aatggttgct gctttaataa ttagaaacca aatacaaaaa aagaaattag
     3121 atacaaaact agcaatacaa ttgatggtaa aaaattagta tccttatact atgattatgc
     3181 agcctcaatt cagccttaat ttatttaaag tctagggcta tagaatgtag tcttgaacga
     3241 ctatctaaca taacttatat taaaaataag attaaatata ctaatggttt tatctaaata
     3301 ttgcttatca aaaagaatgc gtattcaata atttcaattt caattactat gacattaata
     3361 ttaggagtaa tatcttcaaa tgatgaagat ttttcagcac tttcccaaca attgcctact
     3421 gatcaaacac aactacagga tttaatatca aaaagtctag attatgttca tcagggtgat
     3481 ataagtacaa catctacaaa tattttgctc aatggaataa atttgactca aggcgaattt
     3541 attttactct atgattccac accctttgga tctaaaggcc atatagcggt taatttacca
     3601 tgtaatcccg ataatcctca tgatccaatt ttcgacattc ttgtcggacg cgctcctgac
     3661 ttgtttgttt tgcctctagg atatttggaa caaatatcga agccgggcaa gatgtgtgta
     3721 tatcatggac aatttggatt tggagacccg ataacagatc ttattttaaa atacgtcggt
     3781 gatgatccaa taaaactaga aggtcctcac tctatagtaa tctcagctca tgaattctat
     3841 aacccaacta ctgaatcgta ttttgaacgt tctcataatg caactaatta ataaaattat
     3901 atttattttt gatacttatt gtattactgc aacatatgtt acattcccta ttatacaacc
     3961 ctacggatgt tctataatag acagttgcaa gtagattgtt ttactgtaaa gtctccataa
     4021 atatagaaac atcttaattt tttagagttt tcaaaggttc tttcttctct aatcatttca
     4081 ttttttaata tttccctgat aatatttagc tgatagcata tatttgccaa ctctctattt
     4141 caggagattt ttgaagtaaa aggaccaaag aaataatctc catgtaaatc atttttaatc
     4201 attttaatta tattttcatc aaatatggtt tcagatttgt tatattttca gttatttatt
     4261 ctataatata atgacatctc agatttagtg aaagaactta attaaaggat gaggtgtagg
     4321 aaagaaacaa tgtgtaagaa tatattctca attgatattt tggagagcta ctatttcatt
     4381 actatgatga ttaattctaa actaatgacc aaatagtcta atttttctcc atttattatt
     4441 ctttaatata ttttttatac ggactagcaa attagtttta gcattttaga ctgtaaatta
     4501 gtggcattgt tcattataag tcacatctat ataaaatcaa aagatcataa attttattta
     4561 tattcgattt atagtctcaa tgctttaaaa gaatgttgaa caattaagtt gttatagtct
     4621 gtatcctatt ataaaatcgt taacaagaat atatataaaa ttatagtgaa aacctagata
     4681 aaaaaataat actataatgt atgtaacctc tttaatacta attataacat attatttaat
     4741 gataaactat attgtattat tatcattagt ttttttaacg atatcttttt ttatatatag
     4801 tataaaagct gaaatcgttc ctatttttgc agaatctcca actgttaaaa tatttccaaa
     4861 ttgcggagcc ttaagtgatc ataaaatctt ttttacagtt actagtttta gtcctaacgg
     4921 gaatgttcat tgggaattta ttgattctaa aggaaatata gattcatatg gttattttga
     4981 aactgatgaa tttggtggtt ttgaagaata tataattgct gaggtagtaa aacctgatat
     5041 ctatacttta aggttttttg atgataaaaa taataatttt ttaaaagatt ttaacggatc
     5101 agaagttata ataaattatc aaataccttg tgctaatcct attttttaga atcaaactac
     5161 actactcaat tattggttga tatataatat cagtcatttg aaagttatta gaaaataaat
     5221 cgtacaaatc ccaattcgga gtctttctcc tatgaaaacc actatatttt tggaatttta
     5281 aaatttagga atttttgcga cttcaacaaa ttttgttttt ggggattccg attttgtcct
     5341 tgaattaaag tatgtaaaaa atatagtaat atgcaacatc tttaaccttt gttgtacata
     5401 aataattatg agttcttcag aggaaaatag taatcaaaat agtagcgagg aattagaatc
     5461 agcacaaaaa aatcagaaaa aggtaggctc tgcagaatca gatccttcaa ctacaggtcc
     5521 cgcagaaaat ttacgagagg aagctgcaca aaatacaggt gaagatgatt ctaaagaacc
     5581 agcataaaca ttttaaattt ttttttgttt ataacaaaaa attaactgca tctgtttata
     5641 tgattaatga ttaatcttga atgaataata aattattaca aagtagataa acaatatttt
     5701 aaaattaaca agtatttcaa ttatctttta tgttaaaaat ttcatattta ttttaatctt
     5761 ttgataaaat tcttgtcaag aattttaaat aatgacgtta gcctattttg ttgtagatgt
     5821 tataacatat tactaaaata tttgttttaa aatatttgtt taattcattg gatcattatt
     5881 tttataacat agatttttat ttatatagta gggtttagag aaatgatcta tttatgatta
     5941 aaactaagtg tctttattat cccaaagata tttcagatgg agagagaata ttaataacta
     6001 gatattggcc tagaggaata aaaaaagaac atttcgatag atgggataaa gaattatctc
     6061 cctcaagaga tttattaaaa aaatataaga ataataaaat cgattttcaa acatttgaaa
     6121 gattattcct tgaggaaatt gaaaaatcta acgctattga catctgtaaa gagatcgtaa
     6181 ataaaaatat gaaaggaaaa aatattaccc tattatgtta tgaaaaagac gaagaaattt
     6241 gtcatagaaa atttgttaaa aatatatgcg agcaagaatt gttaaaaata aacaattaaa
     6301 ctttatttaa taaaatcaaa ttaaatgcat tcagtattat aatttaaccg attcaattta
     6361 acggctccgc aaatagtatc catgttttct taatcctggt atatattgaa tgatacctaa
     6421 tttctcatcc tccgtattaa aaaggatttt tataatgagc tagcgtacac aggagaataa
     6481 ccaactgttt caatataata ctatagtcct aaataaataa ctaacttata ttgatatata
     6541 gcgatttaga tgagcaagat acggtattat caaatgaatt tagattgaat gtaaatatca
     6601 gattttaatt atatgctacc aagacatcta cttattttca cgtcagtgtt aataaaaaaa
     6661 ttccctttat ttaaatgcat aattatttta tttattctaa ttttaagctg ccttgagagc
     6721 attcatttaa ttttttattt aaacccgact ctttagtaga atattagacc ctttgtatta
     6781 tcttatttgg tcatctatat tatttttccc cttaatattg ctttcacgaa gagaaaaaaa
     6841 tggaatcatg atgtaaattt tattgtatag taataaataa taattaaaca taaacatact
     6901 cttttattct tgtttgtgca tatgttattg aatatgaata atattcaaat ttgggagcat
     6961 caacgtatat gtttttagat atatttataa ttgctagagt ttttaatata ctctatacat
     7021 ggttggaaca caatataata gatcatattt tgaattttag taaagtattt gggaatatga
     7081 tagtcttgat tagtgtggtt ttatttctac ttatacttgc atacattaca ataaaacatc
     7141 taaagaaatt accagagttt tattcaatag atattacaga tatcgatgga aataacgtcg
     7201 ttatagaagg aataagaact aaatttacaa catatgatgc tgctaaaagt tattcagaat
     7261 tttatcatga tatatataag aaacaataca agtttcgtgt agttggtagc aatcaaagat
     7321 atactttggg atttttggat gttgtatctt tacctaagag ggcgtctaaa gcaaaagaga
     7381 ttaactcata aattcttgtt acgggcataa catcttgcta cgtcttggag tagtagtaag
     7441 tgattatctt accaaataat tgtgattccc tcatctttgc atgtccattt atcaagtacc
     7501 cgaaagataa aactgaatct atgcgattta tttattggaa tctagatata tctattttat
     7561 aactttctta gaatctgtat tatgttggtg atatattgct gaatttaact agccaatagg
     7621 ataatatatt cgataaaaat ttaatgtcga atatgaaatt ttagacattc ctcatttgta
     7681 taaatagtaa aacagatatc aaatatggaa taatttatat aataatatct ttaccctttt
     7741 tattctaatg atctgaagtc atttaatccg taactgcaaa atttgtattt gtaatatctg
     7801 agaaatggcc attaaagcca ccatttttat aatgaaaggt tcattcattg agaatctata
     7861 tacatgatct ctcgtcctgg ttttttagtc caaagaacac cagacagaac cataactatt
     7921 ttttattcaa cattgtcaaa gaaagttcta ctattcttcc ttttatttta aattctttaa
     7981 gttctaccga ttcctaggga ttatgacgaa aaatgaaatt gagatgatga tggtggagat
     8041 cttgatttta gtgaagattt taaagtaata tatttagaat tttaaattat tgtggttaat
     8101 tttaacaatc tgaattaatt aaaagaatca taacaatagt tatggtaatt ggcgtaattc
     8161 atctcatgaa tctatgtttc agtcgtgatg caaaacttgt ggtgatagaa ttggaaaaag
     8221 actataaatt aattataaca attacaaaaa aattagttgg ttggtgaaac ttttccttac
     8281 taaaaattaa attaattaag aatattattg ttataaagtt ttggtcataa aatcattttt
     8341 aattattatt ctaattaatc ttacttaaaa taataatgat agaccaaagt tattattata
     8401 ataaaatcat cattgtataa aagatatgat caaaaatcag aataataaat aaattgattg
     8461 caactaatac atctatctta gtatatcgac caacagaaat tatcaactga attatatttt
     8521 tgtatgtaaa taatattttt tactgtttag gaatgaagtt tattttacat gttcttttca
     8581 aaggtttcaa tatatatttt atatgccatt actaaatttt tatagttagt tattgcatta
     8641 tcgtagactt taaccaggtc agtattagat ttataacctg aagtgtcttt aattaactct
     8701 agtcctttat caatattttt tatttcattt tccagatctt taactttcgt tttagcattt
     8761 gaaatttcat ccattggtga atccgtagac atcatatatc acatttaata attatcttat
     8821 ttttattctc ctataaattt taactataaa ttttgtaatt cgaataaata tagaattaac
     8881 taaatgataa cttatattct actatattaa tcgtttaaga attatgtctt ttttgcaacc
     8941 aagagttaaa aaatatagtg aacacaatta cttaaatgag gagatttatt caaaatttat
     9001 atttgtataa tataacttaa ctagtaattg gaatattctg gaagtatcaa atgtattatt
     9061 tgttcagtta cccaaaaatc taattacgaa acagttttat aatagtagat ttagtaatac
     9121 taaaaatgaa tctcatctat aataatgatt tttgttcatt aaaaaaaagg cttaggcaaa
     9181 aattggttga agaggaacaa ccatctccat tagaaacaaa gtcataatat tttcataata
     9241 gaattttttg taacacaaag ttaaatattt cagtaattgt aacatgtaga gtaatgattg
     9301 atatttctat gttcaaaaaa atttactttc agtatatttt tatttgattt tgttgagtat
     9361 tcgttttgat tgttgttctt ccatttaaaa caagtaccct tttttttgca taatattatt
     9421 gtcttttata atttatttgg ttattgtaaa tgtgtatatg cctatagaat ggaatttcaa
     9481 aatatgattg actggagcaa ttgtgtaagg aaaaagataa ttgtaaaaaa attaatagta
     9541 ttatataatt taaccaaaga atacttaatt ttaatatctg caaaactccg gtcctaactg
     9601 catttctaaa aaatatatat attgaagagt taaaattaga ggttaagaaa ttaccctacc
     9661 tttttaatat ttctaattaa atcttgtttc atttgattaa actctatttc agttagtatt
     9721 ccttcttctt tcagttttgc aattttctgt aattcatctg caattgatac tgaaggatta
     9781 gacgatattg tatgatcctt tggttcaaga aacttggagt gtatatgttt ttttgaaggt
     9841 gctgacttac ggccatcctg gcttcctgaa cgaattattt ctaataaatc ttctgcctta
     9901 tcttttggaa tagcttcgat tatacctgtt tgatcatctt caccttcaat tatgctatcc
     9961 atcattccta atttggttga gctcattaat ccaggagctt taaatcttat tgtagatgat
    10021 agcaatccct tttcaagctt taaacttgta attatatcgt aaggaatatc cactacattt
    10081 gcttttatcc caagcatata tggatctctg attatgattc ttctgtctgt tgcatagatg
    10141 gtatttggtg taaaaaaaga gcctccaggc tttatttttg actgttttgc cacaagtaat
    10201 actttttcgt ctggatttaa catctcattt attttgttaa cttcttttac atcgtgttta
    10261 tctgtaatgg actttttaat atggtgagaa tattctttat ctttatcctc tttgctgcta
    10321 ctgctaaagc caaagggcat attatttcta ttattagaaa gagaactatt caatatagaa
    10381 atttctaaaa ttttttaaat attaatctta gtgttattgt attagtataa aatattacga
    10441 tgatttactt tttttataga tagataaata ataatcaaat acatcttaca taaaccattt
    10501 caacctatat tcacttttac ctttaaagtt cagttccata tgggatagaa tatattatta
    10561 gaatgcatgc cttttaaaat acattgtaat tagaatagaa taatataata gataaatagt
    10621 aaaatgtaaa tcagtaactc actatcaatt tttgtatttc tgtttttaat tcatcaagaa
    10681 atacaggctt ttctaatatt ttgtcaattt tcataatata ggatatttca tatggaatat
    10741 cagtttttgt gtatgcacta acaattattg tttttatttt gtgatcagta tctttttctc
    10801 ttattttttt taaaaaatct aatcctgaca tttgcggcat tctataatct gtaataacca
    10861 cagaacaatt gtttgtattt ttataaaaat attctaatgc tttaactgga ttatcaaacg
    10921 aaatgatgtt ataaccattt gattttaaat aatcactaaa taaatcaaga acatcttttt
    10981 catcatctac caataatatg taataattat tattattatt tgatatttta aagaaactcc
    11041 attagtacta tagtattgtt taatttatta ttaaatagtt ctatttcgtt tcaattttat
    11101 aaaattggca ttaccgttag attagatata ttatatcaat aaatggatgt atataaccat
    11161 taaaaaatca ctataggtct attccagaaa ctttttacaa tcttttaaaa gttctcagtt
    11221 gtatctttga tacattttta aaattctcat cgattatgag atttctaagc atatgaagaa
    11281 tgatctcgcc tttttgttgg aatgactgtc aaacaattca ccggtatgct tatacaaatt
    11341 agtgactata tatcttttgt ataagctgct ggtatcaaca atataattaa caatcttttg
    11401 aataaattag tacaaatttc ctcatttgtc tttttttaaa tttaaattta tatttatatt
    11461 tatatttatg aacccaaata aaagatataa aaaaatctgg aacactttac aatatatgtg
    11521 atcatcactt ataacattgt ccttcagtta aaataaatta tttgaaataa tacaggatga
    11581 acaatagata gaaaccaata tcttgaccct tttgtttcac cttgtggcca gcattgaatt
    11641 gcagggggat tcattagatt catcttggac accatataat ttaatttgaa tttgttttct
    11701 ggtaagaatt taatatactt cctgaaaatt gcacctttta ttgctaatag ttgaaagatt
    11761 tacaaagtag attgtataaa agctttttac ttaatttcta ttagataatt aaagtgtatt
    11821 ttgttaattt tattttgtta atttataatt taaaaaacaa ttgttattta tatataaatt
    11881 ttgattcaag gacgtataac aaagtaagag taagaaaata tattatgatc aaaaataata
    11941 taaaagtata cgacaatagt taaatgattt aatgtccaat aaattatatg atttggacat
    12001 taatatcaat cgttgcagga ataataattt taatactgtt gttaagattt ctgtttggaa
    12061 taatctagaa gtggatatga atgatgaatt tgacctcaaa atttattcag gccgcacttg
    12121 ctctctcatt ttctagggta gaagaaatca aaaataacag aattaaagaa ttacttgaaa
    12181 tgaaatcaaa aataataaat gatcctctag tagtagaaaa ttggtttgat aatgaaatta
    12241 ataaaataat aaacgtaaca ggaactagtt ctcattctac tgatcttgaa tctataataa
    12301 ataaaataaa ataattttgt atgcaactat agcgtttcaa acattgaaac atacgacaat
    12361 acagttgatt tgctattaga ataattttct aatcctattg cttaataaat ggtatcttta
    12421 acatcaagcc tttaaatctt caactaaagg gtatttctat caaccattag tattgaaatt
    12481 aatgtttgat ctttattcaa taaatctagc ctgtattgtt tttggatttt tattggtaaa
    12541 tttttttctt acccttattc tagtttctta ggctacttgt tctttttgga cttcatttat
    12601 ttctaaatta atattttgat ttcataattc attatacctc ttctagctct attatctttt
    12661 attttgcgag aattgctcca ttccaaagtc tatcaaaaaa gtactactgt ggttctacaa
    12721 ctattctaat attactataa atgatatctg gattaatatt tttatttttt acatcaaaat
    12781 tagatcaagc gatttattga ctcacttatc aacaacttgc tttttatttc atttaaaata
    12841 tctaaggtaa tcaactattt aagttcaata caatattaat ttatagatat tactattttg
    12901 taaatgtaat taatatttaa attaaattat taattatcat atcataacta taaaatttat
    12961 ccaagatgta aattaaagct aactatcagt ttgataataa atttgctaag agcgtattca
    13021 actactattt tttaacacta tttgtaattc atatacgaaa cataaaatgt tacgaggcat
    13081 caagccttac ttttaatatg ttattaaaca aaataacaat gaacttcgat tattattata
    13141 aaagttaact gataacagat aatttctaat ttatctccta caaaaattag tataagagat
    13201 taacttaata ttacaaatat agtatagaag agcattcccc ataaaaaaat ttaatctttt
    13261 actctaatta aatcatatca tatataaaaa ttttcaatca ttaacaataa ataatgtagt
    13321 ttataaaaaa agatgtgaac aataatttta tcattgtttc attactggta atatttacag
    13381 tatgtattac attatcctca attattgggc ttaatgtaga gggacagaga gaaaatttca
    13441 atgttattaa cactgataaa tctaatatca gtaatataga gaactttgaa acatatgaaa
    13501 actcgaaata tgggataaaa atagaatatc cttctaattg gctaaagact gaaccaggtt
    13561 ctactattgg catagaccct tataaacaaa taaatgttgt aacttttagt tctcctccta
    13621 ataacgaccc tgtgttaatg gttatagtgg caaataaaac tgctggaaca ttagtagatt
    13681 ttgcaagtgg tgggattaac caacatagaa taatatatcc tgattttaat cttttaaagt
    13741 tgggaaatgt caatctcggt gataatcctg ggtataactt taccttcagt gcatctgatg
    13801 caaatggaaa attcaaagct gcacagatat ggactatcaa agataataaa ttatatttac
    13861 ttgtaaatac agcgagtgaa catctgtatc ctttaacttg gccaactata caaaaaatga
    13921 tagagtcgtt tgaaattgta aattaattat actaataatc taatgcttct aatgcaattt
    13981 aattccatca aacagcagta ttctaaatac agagacatca tctcttttaa caaagtatca
    14041 ttattgctgc aataataaca gcattgcaga cgtatttatt ataaattatg catcaataat
    14101 atacttcaca gactatttac ttatctcaaa tatttctctt ttgcccgatt ttttgattta
    14161 taatatttca tttattatat ttcttaaatt ataatctaaa tatatcaatt tagatggacg
    14221 taaaaataag caaaaaatta agcaagattt atagaaacta ataactataa ttggatttgc
    14281 agagatttct tatcctacta ctaaattctt ttctactttt ttaatttttg aatcaacgtc
    14341 aattgatccg tcacttatct caattattac aactgtctta gcatggatat tctatatatt
    14401 aattgcaaat ataatggcaa aaaacaaaaa actattcagt tagatcccaa attggttagt
    14461 gagttgattc tgaatactga tggttattat tctttaaatg tagtttgtaa taattcttcc
    14521 atttcgttat gctttttgag atagtttaat cctttttctg tagtcttgaa agtttgtgtg
    14581 ccatctagaa attctattag gttgttttcg attaatatag aaagatattc ttttaattgt
    14641 ccataactta gaaaagcttt atacataatt ttcgtcttgg tcgctccgcc attagctgag
    14701 tccagtatat tacctactat ttcagttctg cttctatttt tcatttatat tacaacctct
    14761 aaatacatat ttctgtctaa ttataactta taacccatca tatatatcgg ttttcagtta
    14821 ctctactatt ataaataata ttataaataa tattgtaata ctatacaatt agttatataa
    14881 aattggtact ctatttttta tttttattta ttactaatag gaatcattta ctttaaatta
    14941 actttaattt gcgtggataa ggaattagtt tcgtgaacat ctttgcattt ttaaccagaa
    15001 accacctcta tagccttcta aaaacttagt aacaatagaa aaatgttcga gtctatattc
    15061 gttaaaatct taggtagctg ttttgcaact caaaaaatca catatgttaa tacaagtata
    15121 atactacaat tacatacata ttttatagaa tgcattatgg agatacttta gtaatacatt
    15181 tacaacatat agatgaaaat tataaacgat actagatttg gagttagtga tggcttttat
    15241 caagttatat tgtagctaga aatgaaaagg ttcatttcca ctggatcaga taatgcaaaa
    15301 aaaatagaat tatttttgat gatttagttc tcctaattta ttctcatttt attttattga
    15361 ttagacaaac aatcttaata tggccttttg ttttaattta acgagtgagc aataatgaaa
    15421 agggattacc agagaaatca cctgataaat taaaaaaaat aaaagattac ggaattcctc
    15481 taggaattat ggctatgtca cttgttatga tttatatatt atgggcttcc ttcggatata
    15541 taggaccttc attttctagt caagttttat cacatcagca agaaacactt cgtgaaaaat
    15601 ataatctacc ttctttagaa gatgaagata gtagtgatac cagcgaagtt cctccttctc
    15661 ttcgtaaagt agtggagtca aatacaaccc caacttccac ttcacaaaat agccaatcaa
    15721 aaatgacaac acagtcatca acaaacacta cgatatccga atcctcagga aatgttacat
    15781 cgaaatcaac taaagaagaa ccatcacaaa caacaacaac ggcagcagca ggtcttacat
    15841 tgacaatcct tgaaggttca tcaatacagg gtaatccaga ctatgaccca gatgaactaa
    15901 cagtcaaaaa aggtgagaca atacttgttg acaatgtaga tactatgcct catactgtta
    15961 caaatggtga aagtggatca gacccaaatt ctggcaagct ttttgatacg agtataataa
    16021 atggtggtga ttcagctgat atggatacat caactattga tgcaggaaca tatccatatt
    16081 attgcatggt acatccatac atgacaggga gtttaacaat agaataattc actaattatt
    16141 gatatatcaa tagggtttta cttttttact tttttatagg tatttcttta attaattttc
    16201 tttattgaat tttatcttta tacttaaaga tattagatta ataaaacaaa tatgcctaat
    16261 gtattatatt atcacttatg acaaatctga agtcaaacaa cagaaacaaa tttctaaaat
    16321 agacttagaa aatgatacca aatatggttg gatttagaaa atcctactct cgaagaaatt
    16381 tttaattttt tactttattc acaacaagaa tcagaaataa aaaaatctaa aacacataaa
    16441 atatttaaaa attatctatg atatcgtaaa tgaattatta gatttgatgg aatctcataa
    16501 ggatacaatt aactctataa gagaacttta cattgcttat gtatcattgc aaatgaatga
    16561 taccataaaa acattaacca tattttctgt tatacttttg cctttaacgt ttattactgg
    16621 tttttatgga atgaatggaa tagatttaac taatttcttt agtttaccct ctggcttgat
    16681 tatggtttta gttagtatgg gaataatact tgcagtattg attatacgtt caaggcatta
    16741 tttacaaatt tctcatattt ttcttttgga gaaagtccaa caattctggc tacttcattg
    16801 ccatttttaa ataatactaa tgaaggtata gattgaatat tgtatttcat tgcaatttct
    16861 tgattttgat caacattgag tttggacact tttacttttc cttctagaga ttgagctaat
    16921 tgctcaacaa ttggacctac cattctacaa gggccacacc attcagccca aaagtcaact
    16981 aagactggaa tatccgaatt taaaactttt tcgctccatt gcgttgcatt caatggaata
    17041 actttttcta attcttttgt cataatatat attattttat taagatggat atagacttac
    17101 aaatgcatat aaagtcacaa aataaaacaa atcaactggt attttttaat ttaataatct
    17161 caagtattaa aaatttaaac tatttcagtt tatgagttta ctctttcata atatctttag
    17221 ccataaggta tttttttaca tattcttgat gattgccttg taaaataatt attccatttt
    17281 tatatgttcc tccagtacca atattttttt ttaattctcg taagataatt cttataaaat
    17341 ctttatcctt taaaccttgg ataattgtaa ttggtttatt gaattttttt atgtctgtgt
    17401 aaatgaaaat atttgatgta tctttatcta gttcttttaa agcataatct atagaattca
    17461 cattttcatc atatataaac atcaattgat attgtaagtt attatacata ttgactcact
    17521 tttatttttc aagtcatagt cacttcaata ccttgaaatt tctctatcgt tgaatacttt
    17581 gcattctctt ggttaccatc tttgaaaatc tctttactac aatattgcat cgaatatgct
    17641 gccatcatat ctataattgg ttgttgcaaa gctattcctt tcttggtagg atagtattca
    17701 attcggatag gtgtttcatt tggaaagact tttctctgta tccatcccat tttctcgatc
    17761 ttttagtctt attgataagg tttttggatt tttaccttct attgaattaa aaattggtta
    17821 aaccttgttt gtttatcatt gatcatattt ctaatatatt ccaaatttct tctatatgat
    17881 tcaataatga taccattata tagtaattat attatattta ttaaaaaaaa tacatggagt
    17941 cacttaaaat atttccaata attaacttat ttgatgtcaa ttagttattt tattaaaatc
    18001 cttattattt ataggatata tgaataaatt attatttttc attcctatat taccaattga
    18061 ttctataatt cttgtagaat aactaaaagc tttggatata ttcaatcgat tgtcctcatt
    18121 tgtttctatt atgatattat tgttagataa atggactact atttccataa aatcttctat
    18181 atcttgccat tttaattgat atcctctgat attttgaatc atcccgatcc atcttaaact
    18241 accaaaatat ttgcctgaca gattctgaac tatttgttga ttagggattt gaaaaggaac
    18301 agataattga ataatttgag tatattttgt ttgattattt cttttttcat ctaataatct
    18361 ctttaacttt gaaaaaatta atatcccttt tttatttaaa ttatatcctg atttgttttt
    18421 atcaactaat cccaattctt ttaatcgctt cagagatctt gataaaattt cttgatgtaa
    18481 attcgatcta acctttaatc cattaaatgt aaatgtggct attgtgtttt catctccatt
    18541 atcaaataaa atttctagaa tatctatgtc actctgtcga agatcattaa tagatataaa
    18601 gtcatcttca taaactttct gtttatttaa tgattctaat aatatagatt tattgtcatt
    18661 atccatagta tttaattttt cacatctttt attagcttgt atttttaatt tacatatcac
    18721 actaactata ttaatgtaag tatatataaa gtggtaagta gcacagaata cagtaactta
    18781 ctatttgtta agcaaagaat tgcttataat atatttaatt atatagaaat caaaaaatga
    18841 ttatggttga ttctctaatg tcactgaaat agtaaatcgt cttccatatc tatttacaga
    18901 taatacagtt tgctcattta cattcttatt tttgattcga gatgaaagct ctgttatatc
    18961 ttctatcctc ttttcatcaa tttcctcaat aatatctccc ggtcttattc ctgaatcaga
    19021 tgctggacta tattcttcta cttttacaac taatgctcct tcattagttg gtaatcgata
    19081 atatcttgct attctactag ttatcttgag agtagtaata cctaaccatg ggcgattaac
    19141 tttcccgttt tttataagct gttgtaatat tgtttttgct gtattaactg gaacagcaaa
    19201 tcctattcca tgggcataag gtattttggc ggtattgatt gcaattacct ctccattggt
    19261 attcactaaa ggaccaccgg aatttccagg atttatagct gcgtcagttt gaaccaattc
    19321 taatactcca tcttcaaatt caatgttcct atttaaagaa ctaataattc cagttgtaac
    19381 tgtaggtcct cctgtcaaac caaaaggatt acctactgct ataactattt gaccaacttt
    19441 taaattatca gaatctccta attccgcaga aggaattaag tctattgctt tattgtcaga
    19501 attgttcgta ttattattgc tattactgga attattagaa tcatgttcaa tttttaaaac
    19561 tgcgagatca gtaagtttat ctgttcctat tactttagca ttgaatgttc t
//
LOCUS       DASVEV010000170        17960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1232932, whole genome shotgun sequence.
ACCESSION   DASVEV010000170 DASVEV010000000
VERSION     DASVEV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232932"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..610
                     /locus_tag="VF222_07775"
     CDS             374..610
                     /locus_tag="VF222_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7142361.1"
                     /translation="MPKAYILINCESGYEGLVANGIKSLDNVLTCRIVYGAYDIIVEI
                     IFDTLKQLEYIIKKRIRKISKIQSTMTLIPFEFN"
     gene            1018..1155
                     /locus_tag="VF222_07780"
     CDS             1018..1155
                     /locus_tag="VF222_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142362.1"
                     /translation="MEGAIKKMLENKVKKFNNLELHLSYIVLAGKPANEIIKFSEESD
                     I"
     gene            complement(1309..1650)
                     /locus_tag="VF222_07785"
     CDS             complement(1309..1650)
                     /locus_tag="VF222_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142363.1"
                     /translation="MIDSDRNSRSSKDIDRYYPITIIKDSNLQLSLVPSVSSQHQLSS
                     SIKIAIWKDERLEHLGISRDLIEILQINGFTIERILEYGPSQIAKIIGIDDIFAQIIF
                     NKTTKRLINEL"
     gene            complement(1820..2962)
                     /locus_tag="VF222_07790"
     CDS             complement(1820..2962)
                     /locus_tag="VF222_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142364.1"
                     /translation="MWHHSNKGNKSVKFTDDSIKCCVCFIEFESAIHNTAIADDSSNI
                     REYYSTVINSLSEIIKRYNGKIVKSLGDRLLCYFLNFSDLNNEKAFEDVIECGLEILE
                     KREDMNKELLKNNNLPPIESYKISIDYGIIDLALAGDNYQLDIFGSAVNICSKINSSS
                     SLAPKNEIIIGNNFYRILKSFPNILNNYNFVNNGELKVTENIGYPTYTIERRSNTLLQ
                     NDDTNSTHSIMMEKQPIIYEDFIENRNNKREGFFFHKKKKNKRIIIVDDDEDILFTYR
                     RFLKDYDYHITCFTDPTLILNYVGDLSNFDDLLIILDIRMKNLNGLQLHQKIKSIDPT
                     IKILFVTALDILDELLNICPGISKGQIMRKPVDKKTFTNTVKNMLS"
     gene            complement(2949..3113)
                     /locus_tag="VF222_07795"
     CDS             complement(2949..3113)
                     /locus_tag="VF222_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142365.1"
                     /translation="MQNPLPNNRNPKSKRETNATVVKTLFPRINVEDELCSSSISFSI
                     IGTRQTNVAS"
     gene            complement(4085..5155)
                     /locus_tag="VF222_07800"
     CDS             complement(4085..5155)
                     /locus_tag="VF222_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142366.1"
                     /translation="MILKIVILIALAVIIGLLKNYYLIIGSVIIVTLFLILKKYKYTL
                     VLIIIFGISILTSTLVSEITKIQLLPFAPVEEKEGIYPIYRDNPDGEYYRFNNKNPND
                     FRQIDEIKHKEIFSEKNPDGSWKVGKGTTRIDISTKQAGILTQEEMMKQIDTWNFSIL
                     DKQGYWLYPSDFKNIEFTVIFKMLESEKRDQAISVVSRSIIHDSKGEEEIKEYPNAYC
                     GGSSYHNNISANGKLQMKKEQYHADYVIDKPNEKIDLGNLYNKKIGFKAIVYNFDNNS
                     KIKLESFVDTKNEGKGPYVKLHEKVDDGKWGINSGKAKMNECGAITKGAIISWGSPKM
                     ILKTNNLDFDLYDIEVKEIIPP"
     gene            complement(5405..5707)
                     /locus_tag="VF222_07805"
     CDS             complement(5405..5707)
                     /locus_tag="VF222_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142367.1"
                     /translation="MKSVIESIFLTVVFVFSMITLLPVYGQETITTNSITDLIKTTAE
                     KIKNVASDVANNSTGIISPSEAKNILNQLGEASKKIALSGTDVLSNFSGEIREGLK"
     gene            5978..6679
                     /locus_tag="VF222_07810"
     CDS             5978..6679
                     /locus_tag="VF222_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142368.1"
                     /translation="MNLLPSIDVIQKNFVSVILLSSIILVIFNTYTHSVFAEEQNLKN
                     MGNYQLSSSNFSSQQEKELFLQKYLTFSAPKEIKEVPNHVSAISTLNDLVNELVTGVK
                     LENIITAPNEVHVGNTFEVNATVINTLPFNVTIIAGNCNSDFFTTFNNNVEEIMTPQC
                     MNRVLPTVLEPGDKTTVQERSGTGKQYKAVAEGDANGTAILSYSIEVKPEGSYLTTTS
                     LNSQVVKPFSFTILP"
     gene            complement(8332..9147)
                     /locus_tag="VF222_07815"
     CDS             complement(8332..9147)
                     /locus_tag="VF222_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7142369.1"
                     /translation="MSFQNLNDDSSSSLHNESIISQFTKQAIPFTQLSEHSNQYGFEL
                     IVELAKPQKGDTVLDVACGPGIVACELAKFVSHGTGIDITPAMIEQAKQMQKERKLNN
                     ITWKIGDIITLPFDDSSFSLVITRYSFHHLLNPPKVLDEMIRVCKPDGRIVIIDVTPE
                     SSKANEYNSVEKLRDPSHVKAFTFRELSKMMEESGLVNLETKCQNLEMELEKLLEASF
                     PPHGNKEKIQHLFKEDLQKDNLGMKSFLQENNNNNNNKIYFYFPISMLLGYKK"
     gene            9486..10379
                     /locus_tag="VF222_07820"
     CDS             9486..10379
                     /locus_tag="VF222_07820"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7142370.1"
                     /translation="MNNKRHQQEKVAVVTGSSTGIGFETSLLLSRKGFYTYATMRNLN
                     KSQKINDVAKSENLPLKVLQLDVTGDKSVKDAINQIKDESSRIDVLVNNAGYGVMGAV
                     EDLSLEEFKSQFETNFFGVIRVTKEVIPIMRNQGSGNIINVSSVGGKIGIPLNSAYIS
                     SKFALEGLSESMRYELEQFGIDVILIEPGVVKTNFFENADVLVNNKTNNKTSAYSQLT
                     QKLFEGFEPMLKSNSSSLSSDVAEVIYQAIESNNRDVRYPVGKDALSIIKTRENLSDK
                     EFENWIKESIFQQKGFIHQEI"
     gene            11015..11692
                     /locus_tag="VF222_07825"
     CDS             11015..11692
                     /locus_tag="VF222_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7142371.1"
                     /translation="MPFGSDDKNEEDNKDSTYFKKPVTEEDDLNEINKINEILNPDEK
                     VLLVAKQSKIKPGGSYFTPNIIYATDRRIIIRDPYMLGIKANVVDIPYDIITSLKLEK
                     GLLSSTIRFKAPGLMSSTKLGMMDSIIEGEDDQTGIIEAIPKDKAEDLLEIIRSGMQD
                     SRKLAASKKLKFIESHEPKETITTSDQSESVADEIRKLAKLKEEGILTDKDFNQMKQD
                     LIKNSKR"
     gene            11925..12083
                     /locus_tag="VF222_07830"
     CDS             11925..12083
                     /locus_tag="VF222_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142372.1"
                     /translation="MATIDQKKDMNKKQEDKDTKENQIEETLENASDSVVAESKKEAS
                     QIGDSDRE"
     gene            12225..12347
                     /locus_tag="VF222_07835"
     CDS             12225..12347
                     /locus_tag="VF222_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142373.1"
                     /translation="MSSHHSGAYQCEACDLDFSSEKEFEEHRKKEHSSDTTSNS"
     gene            complement(12344..12514)
                     /locus_tag="VF222_07840"
     CDS             complement(12344..12514)
                     /locus_tag="VF222_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142374.1"
                     /translation="MFHITITKKISTNESNLKNIISKKDGYYNKSILLCAHYDLRIRD
                     KDDYTSRTPGAY"
     gene            12930..13490
                     /locus_tag="VF222_07845"
     CDS             12930..13490
                     /locus_tag="VF222_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142375.1"
                     /translation="MYKETWFKQKLSVSTLVVVGVVSSLIIILAANPIHNAYSINPTN
                     PTNDVIKGSISSISTDPSNSSTAWIITGVYKMENVKTSPTFNSTFYMIKTDGTGKHSH
                     SIYDFKVNADPIVNTSNNSTILNGTSTVTMKEVPINDVPTQITVLGDSAINILLEPTK
                     VNNHFGNGPIYGNQHLICVEVPSYCK"
     gene            complement(13784..14185)
                     /locus_tag="VF222_07850"
     CDS             complement(13784..14185)
                     /locus_tag="VF222_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142376.1"
                     /translation="MLGDLFYEAKGRITSKKVLELEGPTIESSYFLEGKMRDIEVVEI
                     GTFTTIPTSEGAYFVEGKDIVTVKGGNEMATVKAQGISKQKDSSKVVSGSTFYRSSKT
                     GKLSFLNNLVGVHEASVDNDGNIIYKVWEWK"
     gene            14415..14546
                     /locus_tag="VF222_07855"
     CDS             14415..14546
                     /locus_tag="VF222_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142377.1"
                     /translation="MSNCSSSIIDILYKTPVYEPRYNTDSEMCQLILTQFCSSFRTG"
     gene            14717..15052
                     /locus_tag="VF222_07860"
     CDS             14717..15052
                     /locus_tag="VF222_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX7142378.1"
                     /translation="METTNIKKFENRTKSVIRLLAFLEAKKGKESELERILLVLIPPT
                     LNEPGNIAYVPHKSIENPRVFMFDELWINEEAIEEHFKQPHMINLLEKLNPLLERPLE
                     LKRYHEIIL"
     gene            complement(15409..15534)
                     /locus_tag="VF222_07865"
     CDS             complement(15409..15534)
                     /locus_tag="VF222_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142379.1"
                     /translation="MDISTDVVIKWKNIKTDISICDDNTIVIDFDDKATDKHFIG"
     gene            complement(15845..15994)
                     /locus_tag="VF222_07870"
     CDS             complement(15845..15994)
                     /locus_tag="VF222_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142380.1"
                     /translation="MSLIESSGVIVPSCANTCSNTVGIPAISTNTRMDGITIELVFEF
                     IYVFV"
     gene            15996..16130
                     /locus_tag="VF222_07875"
     CDS             15996..16130
                     /locus_tag="VF222_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142381.1"
                     /translation="MSSQNKTFTSRTNTDFSAVNAVNDTMLGENITNGLTLQNLENHN
                     "
     gene            16322..17506
                     /locus_tag="VF222_07880"
     CDS             16322..17506
                     /locus_tag="VF222_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7142382.1"
                     /translation="MFINVNYKEHLRLSIIIVIGFILVLQSFSILTVLAQTTTQNNLT
                     AENLFPKIHEIKMAAVELPDGHTAYKMLEYKVIDSENQTKDITSRYSDLPTIPGPTLI
                     MTEGDKARLTLVNEIGRGAVSLHTHGVHYTITSDGSLKMSNRVSDQGATPNDSYTYVW
                     TAAEGTRGSWPWHDHTFGKGSSGMNMNGVETNGLFSTVIVNPANGKVDALVNGIPKEV
                     NVGDIKKDFVLFVTDDAFWGVEIDNNNNNKHTPLWVNPTLVASKNDLVRFHIQSVGTD
                     FHHFVIDGYEWLKPGTHETTNFENIGPLENHVFTISANNNTSYYDKMPIHLLSGMKGK
                     FQVIEAAAGATTSSIPGPSPQMQDSSNSKDTGNSTGVPATSPIMHGTSDNNGEISLVG
                     SE"
     gene            17508..>17960
                     /locus_tag="VF222_07885"
     CDS             17508..>17960
                     /locus_tag="VF222_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142383.1"
                     /translation="MRNKNRSSNKNCNSKHTTIISSFKPCFILFIILIVATGPISALN
                     LPVVEIASAQQQQQQQVSKDTANNESVTLENNKNIIKDNKKEVFHTLTSIPSSPEGND
                     FSSSSSSPNNKTNNNTMHKIEMVVITLPTGQPAYKMVSHVKSNGSPSSS"
BASE COUNT         6423 a         2569 c         2468 g         6500 t
ORIGIN      
        1 ttctttgctt ttatcaacta ttctaggata acctttgtaa gttttattgt ttatattgaa
       61 ggacacgttt ggatcaaata tgatattttg gacccaatct gaatcttttt tatgctctga
      121 tatcttcttt tacaacttac atatataact tttaacagaa aaattctatc aattaagctg
      181 aaaattctca ttataacagg gttcggtaac ataatataat actttattaa tgaaatttga
      241 gttaatttct ctctatttac aaatttatat ggtcaataat tcaattgagg atgggatttg
      301 aaatcccatt ttgttgtcta tatttcaaag aatcgtaact ttttttagtt tgactttata
      361 tagatagtat tgtatgccta aggcatatat tctaattaat tgcgaatcag gatatgaagg
      421 tcttgttgct aatggtatta aatcactaga taatgtatta acgtgtagga ttgtttatgg
      481 agcatatgat atcatagttg aaataatatt tgatacttta aaacaattag aatatataat
      541 taaaaaaagg attagaaaaa ttagcaaaat acaatctact atgacattga ttccttttga
      601 atttaattga aatgaagttt acctttattt agcaaataac aatattaatg ctcagtacat
      661 taaatatatg attaatattt taaaataata tttgtaaatt tgaatttgaa tcttttaaat
      721 aataagagat ttatatttat atattgaatc gagatacttt tgagggaaaa ctttctgatt
      781 ctttctctgt taatcttaat tttccaacct ataaaaaaat tagttcccca tgatggatca
      841 gaaatatcaa atattgcttt aagacatgca atttatctat ccaaaatctc taaggcttaa
      901 ataattatta ttaacataat tgaggaagat gtaatttctc caagtacact tttatcattt
      961 ataagaaaag aagaagaagg aggattaata caatcaaaag aggatcttcg caatatgatg
     1021 gaaggcgcaa taaaaaaaat gttagaaaat aaagttaaga aattcaataa tcttgaacta
     1081 catttatcat atatagtatt ggcaggtaaa cccgcaaatg aaataataaa gttttcagaa
     1141 gaatcagata tatgatttaa tagttatggc aagtagcaga atatcgtcaa caatacgggt
     1201 aattggaagt acagttagaa aggtaattga tagcactagc aaaccagtct tagtgataca
     1261 tgaatagaat tcttcaaatg ttggaaaatt ttttcggcgc cgaaaatgtt ataactcgtt
     1321 aattaatcgt tttgttgtct tattaaatat tatttgtgca aagatatcat ctattcctat
     1381 tatttttgca atttgtgaag gtccatattc taatattctt tcaattgtaa agccattgat
     1441 ctgtaatatt tcaatcaagt ctcttgaaat acctagatgt tctaatcttt catctttcca
     1501 tattgctatt tttattgatg atgataattg atgttgagat gatactgatg gtacaagaga
     1561 gagttgcaaa ttggagtctt ttattattgt tattgggtaa tatcgatcaa tatctttact
     1621 actccgacta tttctgtcgc tatctatcat cttataatag atataaagta tacatcgtat
     1681 gtattaaagt gatttatcct caaggtatcc ttcaaagatg ttattgaatc ctactaataa
     1741 caggatcgga gatgtgtcaa ctgattttga cacaattttg tagttcaaaa aaaagtattt
     1801 aaaaaataag aatatctttt taacttaaca tattttttac agtatttgtg aatgtttttt
     1861 tatcaactgg ttttctcata atttgtcctt ttgatattcc gggacatata tttaataatt
     1921 catctaatat atccaaagct gtaacaaaca atatcttaat tgttggatct atagatttta
     1981 ttttttgatg taattgaagc ccattcaaat ttttcattcg gatgtcaagt attattaata
     2041 aatcatcaaa atttgaaagg tctcctacat aatttagtat aagagtagga tctgtaaaac
     2101 aagtaatatg ataatcataa tcttttagaa atcttctata agtaaaaaga atatcttcat
     2161 catcatcgac tataattatt cttttgtttt tctttttttt atggaaaaag aaaccttctc
     2221 ttttgttatt cctattttct ataaagtctt catatatgat tggttgtttc tccatcataa
     2281 tagagtgtgt actgttagta tcatcattct gcaacaaagt attacttctt ctttctatgg
     2341 tatatgttgg atacccaatg ttttctgtta cctttaattc accattattt acaaaattat
     2401 aattgtttaa gatattagga aaagatttta aaattctata aaaattattt ccaataatta
     2461 tttcattttt aggagctaaa gaagaagaag aatttatctt tgaacatata tttactgccg
     2521 aaccaaatat atctagttga taattatccc cagctaaggc taagtctatt ataccatagt
     2581 ctatactaat cttgtagctc tctattggtg gtaaattatt attcttcaat aattctttat
     2641 tcatatcctc ccgcttttct aaaatctcta atccacattc aattacatct tcaaatgctt
     2701 tttcattatt aagatctgaa aagttcaaga aataacacaa taaacgatca cctagacttt
     2761 ttacaatttt accgttataa cgtttaatta tttccgataa tgagttgata acagtagaat
     2821 agtattctcg tatatttgaa gaatcatctg ctatagcagt attgtgtata gcactttcaa
     2881 attctataaa gcatacgcaa cattttattg aatcatcagt aaatttgacg cttttattgc
     2941 ctttattgct atgatgccac atttgtttgt ctagtcccta taatactaaa agaaatagaa
     3001 gaagagcata attcatcttc tacatttatc cttggaaata gagtttttac tacagtagca
     3061 tttgtctctc ttttggattt aggatttcta ttatttggta gaggattctg catcattttt
     3121 atcctcctcc tcctaaaatt taggactaaa tatagcattc ataaattata tattatattt
     3181 aaaaatttaa agaaattggt ctaattagaa gatatagatt aaaaagatta aaaatcacaa
     3241 attttcggca cctcaaaatt tgataaaaag ttattaaaaa ttgattccaa taagaatttt
     3301 ctctttagaa tatagcctat ctggttcaaa ttcttctcca taaacgctag ccaatttttt
     3361 gaaaaaaaga aaatagtttt tttttgacaa atcaacttat actgttaata tttattcgtt
     3421 tactgtaagt aaagtttgta tctttttttt accactttac agcatatact agaagatttt
     3481 agtatcgtat tttctagttt tattaaatat aaagcatagg cttcttttaa aatatttttt
     3541 aactaaaatt ctttgtatct tttatgtttt aagatgagta atgaaaagtt attatttatt
     3601 cgaagataga tttaatcgag aaaaatgata ttctttatat tggactttac agtcattgca
     3661 tttataaagt atcatttgct gaccagtcct actttctaat ttattgatat atagtaaatt
     3721 gtgcttgcaa aaggaacatt tcatcaattt tttaacttct caatttttta tttcaaatta
     3781 aagtataaaa atttattatc aaactcaatt tatattagtt ctataatggt tgatattcaa
     3841 gatggatata atatacttgt tttaagccac gtactttttt ggcgcctcaa ttttttttta
     3901 tgttatatta ctttcaatca aatgaatgaa gtattttgac acaattttgt tggtcaaaga
     3961 aagaaatgta attcttgctt aggagtatta atatgatagt ataatttatt gaaatattaa
     4021 aattaaataa tataaggaca gtgacatcaa atggagaatt catctgtaag agtattctct
     4081 cttttcaagg gggaataatt tcttttactt ctatatcata taaatcaaaa tcaaggttat
     4141 ttgtctttaa aatcatctta ggagaacccc atgatataat tgctccttta gttattgctc
     4201 cacattcatt catttttgct tttccagaat taattcccca tttgccatca tcaacttttt
     4261 catgtaattt tacataaggt ccttttcctt cattttttgt atcgacaaaa ctttcaagtt
     4321 ttattttgga attgttatca aaattgtaaa ctattgcttt aaacccaatt tttttattat
     4381 aaagattgcc tagatctatt ttctcattag gtttatctat tacataatct gcatgatatt
     4441 gttctttttt catttgtaat ttaccatttg ctgagatatt attgtgatag cttgaaccac
     4501 cacaataagc attaggatat tctttaattt cttcttctcc tttgctgtca tgtataatag
     4561 aacgagagac aacagaaata gcttgatctc ttttttctga ttccaacatt ttgaatatta
     4621 cagtaaattc tatattcttg aagtcgcttg gatatagcca gtaaccttgt ttgtctaata
     4681 tactaaaatt ccatgtgtca atttgtttca tcatttcctc ctgggtcaag attcctgctt
     4741 gttttgtaga aatatcaatt cttgttgttc ccttacccac tttccagctt ccatctggat
     4801 ttttctctga aaatatttct ttatgtttta tttcatcaat ttgtctaaaa tcgttaggat
     4861 ttttattgtt aaatctataa tattctccat ctgggttatc tctataaatc ggataaattc
     4921 cttctttttc ttctacggga gcaaaaggta gtaattgtat ttttgtaatt tctgaaacta
     4981 atgtacttgt tagtatagag attccaaaga ttataattaa tacaagtgta tatttatact
     5041 tctttaggat taaaaataat gtaacaataa ttacagaacc aatgattagg tagtaatttt
     5101 taagtaatcc aataatcact gctaaagcta ttaaaattac tatctttaaa atcaaccaat
     5161 tcaattatag actcaataaa ttgtatttta gccaatatga gtgttcaatt cccaaaaata
     5221 aatttacttc tttaaaaaat aagttaaata taataacaga ttaatgaatg taaaaatctt
     5281 tttaatataa atactttttt tttgctctga tcaattaaga ttgaatgata aattaattgt
     5341 ctattttata tactagtaat gatcagatgt catatgccta attatatttt ctaatatcta
     5401 ctttctactt tagaccttct ctaatttcgc cactgaaatt agatagtaca tcagttccag
     5461 ataatgctat tttcttagat gcttctccta gctgattaag aatatttttt gcctcactag
     5521 ggcttattat gccagtagaa ttatttgcaa cgtctgatgc cacatttttt attttttctg
     5581 cagtagtttt tattaaatct gttatactgt tcgttgttat tgtttcttgt ccataaactg
     5641 gaagtaaagt tatcatagaa aaaacaaata ccactgtaag aaatattgat tcaattactg
     5701 atttcattgt attcattgat aatgtaataa gaaataagta ttatttagga taatctcttg
     5761 ctagtcagtt gcctaatgaa attaattatt ttgacacaat tttgtagttt ggaaaaaaat
     5821 tatatttaat tgttttataa ttaaataatt ttacttttct aatctcaaat taggaatata
     5881 tagatttcta tagaatttgc taacaatata atttttaatg tattatatcg tcaaaaatat
     5941 ctatatgctt atatagtaac cattattgtg atattacatg aatcttttac cgtctattga
     6001 tgtaatacag aaaaattttg tttccgtcat tttgttatca tcaattattt tagtaatttt
     6061 taatacatat actcattctg tatttgcaga ggaacaaaat ttaaaaaata tgggtaatta
     6121 tcaattgtct tcttcaaatt tctcttcgca acaagaaaaa gaattatttt tacaaaaata
     6181 tttaactttc agtgctccca aagaaataaa agaagttcca aatcatgttt cagcaatctc
     6241 tacattaaat gatttagtaa atgaattagt tactggtgtt aaacttgaaa atataattac
     6301 tgctcccaat gaagttcatg taggtaacac atttgaggta aatgcaactg tgataaatac
     6361 gttaccattc aatgttacga ttattgcagg taattgtaat tctgactttt ttacaacctt
     6421 taataataac gtagaagaaa taatgactcc tcaatgtatg aatagagttt taccaacagt
     6481 acttgaacca ggagataaaa ctacagttca ggaacgtagt ggaacaggga agcaatataa
     6541 agcagtagca gaaggcgacg caaatggaac tgccatttta tcatatagta tagaagttaa
     6601 accagaaggc tcttacctta caacaacatc attgaatagc caagtagtta aaccatttag
     6661 ttttactata cttccataaa cttttatttt atgaaaatta ttgttaaaag gtaactaaat
     6721 tacatctttg ataattaaat atatctcttt ataataagat tatcttttct cctataattc
     6781 tttaaatatt tattcacaca tcaaatttac aaagatgagg aattctcttt tcagctattg
     6841 gagctcttac taagtagaag aagtaacttc actactacta ctacctacta tattgggatc
     6901 ttcaataata tagctttata attagaatat gtaatctaaa gatagtattt tatggaaaca
     6961 tacttagcta gtattttttg taaataaacc tttattgaaa ctactagatt ctgaatagat
     7021 taaaaaaaac agagtgaaag atactagcta gattagtgcc atacctccat caacgaacag
     7081 ttcgataccg gtatatttaa ggaaaaacat atttatccaa atatagtaac atataataag
     7141 gaccccgcaa ttattcctcc aactccagta gcaaagccaa cttactacaa taaagaattt
     7201 tcaaattcaa atgcagtaac aacagcaacg agaataaaat caaagatgga taattatttg
     7261 agtttttgga aggatctcct gttacaatca aaaaaattat gagtttcttt ctgacattat
     7321 aagagattaa aatggaaaag ttcatggaag tcaatctcat atatctaata atactctgca
     7381 tttaattgtc tattatgaag tcctagaagg tcaaagagaa catctaaaga aaaagaatgg
     7441 taactttttt caatataaaa atgaaatggc tatttcagaa tacgctaaaa atctttaatt
     7501 tttaatttca atcatttcta ttgtttttct attaaatcta aaaattgctc taattatttc
     7561 agtaaaaaac ttgacaaagt caatttcatt atcctctaca gcaattaaat gcctttcatt
     7621 ctgtcatcta tcgtttgctt attgctttat tatttccatt cccaaacctt ttcagtagaa
     7681 ataccagatt catttatttc agtttcaaaa actcctatct tgtcgttcaa aaatgaaagt
     7741 tttccatttg ttgatattga tgacttgtaa aatacagatc cacgaaaact agcgcctact
     7801 tgtcctttag aatgtcctat tccatatccc taaatgttgc agtatcttca ccatctttag
     7861 tagttattat tccttgtcct tcaccataaa ttacattttt tacctgtagg aattgcccaa
     7921 aatgctccta aaattgtaat ctcaatatct ttgagttttc cttcgtgtgt tagggaatgt
     7981 tctattttgt attcgtctgc atttaagatt ctagaccctg taagtctact tttatttcaa
     8041 ataataatta tccaagcata gttagttgtt accttaattg catttaatat ataaagaaaa
     8101 ttgaacttaa atcgaaacat ggttaattgg attggtcaaa aaaaaatata gcaggatggt
     8161 aaatcaatta ttttttcata aaaataaatt gttgatttgt aaactaatga atcaagatta
     8221 aaaattactt tcattgataa aaggattctg agggcttcaa gtatggcatg taattgggat
     8281 ctgcttagtg aactttaaag ttaaatatta ttaaccttag aatgattaaa attatttctt
     8341 gtaccctagt aacatagata taggaaagta aaagtaaatc ttattattgt tattattatt
     8401 attttcttga agaaaacttt tcattcccaa attatctttt tgtaaatctt ctttaaacag
     8461 atgttgaatt ttctctttat ttccgtgagg gggaaatgat gcttcaagta atttctccaa
     8521 ttccatttca agattttgac atttagtttc caagttgacc aatcctgact cttccatcat
     8581 tttgcttaat tctctaaaag taaatgcttt tacatgagat ggatcacgta atttttcaac
     8641 actgttgtat tcatttgctt tactagattc aggtgttaca tcaattatta caattcttcc
     8701 atctggctta cagacacgta tcatttcatc caaaactttt ggtggattca ataagtgatg
     8761 aaaactatat cgtgttatta ctaatgagaa tgaagaatca tcaaatggta aagtaatgat
     8821 atccccaatt ttccaagtta tattatttaa ttttctctct ttttgcattt gttttgcttg
     8881 ctcaatcata gctggtgtta tatcaattcc agttccatga gaaacaaatt ttgctagttc
     8941 acatgccact attcctggtc cgcatgcaac atctaatact gtatcgcctt tctgtggttt
     9001 tgctaattca actattaatt caaatccata ctgattagaa tgttctgata gttgggtaaa
     9061 gggaatagcc tgctttgtaa attgagatat aatggattca ttatgtaatg aagaagaaga
     9121 atcatcattt aaattttgaa aactcattaa taataataat tgttatgatg tctttattaa
     9181 aatttcattt gataatgcta ttttatgtgg ttttcatatt ataattatta ttagtaaatg
     9241 caaaactctt aacagatttt taacttattt aatacaaaaa acactttttg gtctattata
     9301 aacttatttt ttatgcgtgc aaatgggggg gggttgtatc aactgatttt gacacaattt
     9361 tgtagttcta taatctcatc atagtattta ttacaatgat ttacaaaata atattaatta
     9421 aactttgtaa tatttttaaa tttgtaaata gctaattata tataatttga aaggttatac
     9481 catttgtgaa taataaaaga catcaacaag agaaggttgc tgttgttact ggcagttcaa
     9541 ctggaatagg atttgaaact tcattattgc tttcaagaaa agggttttat acttatgcaa
     9601 ctatgagaaa tttaaataaa tcacagaaaa taaatgatgt tgctaaaagt gaaaatcttc
     9661 ctttaaaagt tttacaatta gatgtaacag gtgataaatc agttaaagat gcaattaacc
     9721 aaatcaaaga tgaaagctca aggattgatg tattagttaa taatgctggt tatggtgtaa
     9781 tgggagctgt tgaagacttg tcacttgaag aatttaaatc tcagtttgaa accaatttct
     9841 ttggggttat tagagttaca aaagaagtaa taccaataat gagaaatcaa ggaagtggta
     9901 acataataaa tgtaagttct gttggtggaa aaataggtat acctcttaat tcagcgtata
     9961 ttagtagtaa atttgcttta gaaggattat ctgaatcaat gagatatgaa ttggaacaat
    10021 ttggaataga tgttatatta atagaaccag gagtagttaa aactaatttc tttgaaaatg
    10081 cagacgtctt agtaaataat aagacaaata ataaaacttc agcatattca caactaactc
    10141 aaaaactctt tgaaggtttt gaacctatgc ttaaaagtaa ttcgtcgtca ttatcatcag
    10201 atgttgcaga agtaatttac caagcaatag aatccaacaa tcgagatgta agatatcccg
    10261 taggaaagga tgcattgtca attatcaaaa cacgagaaaa tttatcggat aaagaatttg
    10321 aaaattggat aaaggaaagt atctttcaac aaaaaggatt catacatcaa gaaatatagt
    10381 ttattatttt cattttttaa tcaattctca tgtattttcc accttcattt gttttatttg
    10441 actgtaatat tgacctatga tatgccaact aataagcaat tttaaacgaa tgaaagattt
    10501 atgttagcag atctaatgta ttaagttcct tctgtagttt gttataaagc agtgtgtcgg
    10561 gaagttccta atatacttga taagatctat atcttttttt agttaaagat taatcatatg
    10621 aaattgggtg aatgaaatat ttgtgtataa aattattata aaacctatag tttgttttat
    10681 atatatacta aaatataaat ttactatatt accaaatcat tatataataa gatttgattc
    10741 tcactaatac cttcaaatat tataataaca atagttaata atgataacct attatattca
    10801 aataacaagg cctaatatta tgttaccgta ccaatttcac ctgaactttt tagaagttga
    10861 aattatattt gtttaaatat taacaactac tactaatctt taactctaat atgttttaaa
    10921 attattgaat attagaaact tagttataac tcctcaaggt acctagaaag gaccattata
    10981 attttaaata agactttcta agatagattg caatatgcct tttggctccg atgataagaa
    11041 cgaagaagat aataaagatt ctacatattt taaaaaacct gttacagagg aagatgattt
    11101 aaatgaaatt aataaaatta acgagatatt aaatccagat gaaaaagtat tactcgttgc
    11161 aaaacaatca aagataaaac ctggaggctc ttattttaca ccaaatatca tctatgcaac
    11221 agacagaaga atcataatta gggatccata tatgcttgga ataaaagcaa atgtagttga
    11281 tattccatat gatataatta caagtttaaa gcttgaaaaa gggttattat catcaacaat
    11341 aagatttaaa gctcctggat taatgagctc aactaagcta ggaatgatgg atagtataat
    11401 tgaaggcgaa gatgatcaaa caggtataat tgaagctatt ccaaaagata aggcagaaga
    11461 tttgttggaa ataatccgtt caggtatgca agatagccga aaattagccg cttcaaaaaa
    11521 gctaaaattt atcgaatcac acgaacctaa agagactata actacttccg atcagtcaga
    11581 atcagttgca gatgaaatac ggaaacttgc aaagcttaaa gaagaaggga tactaactga
    11641 caaagatttt aatcagatga aacaggatct aatcaagaat agtaagagat aatgcataat
    11701 ctcttttatc ttttggttta tggctagaaa aaaatgatag atactacatc agtacaatat
    11761 attagattat cataaattca tagaaagatt aagagcctta ttactagacg ggctcggagg
    11821 tgtgtcaact gattttgaca caattttgta gttcatctaa taaagaaatt aacaatcatc
    11881 caaatagcaa tacatatttt agatcttaat aatatactat tattatggct actatcgacc
    11941 aaaagaaaga tatgaataaa aaacaagaag acaaagatac taaagaaaat caaattgaag
    12001 agacacttga aaatgcttct gatagtgtag tagcagaatc aaaaaaagag gctagtcaaa
    12061 taggagattc ggatcgtgaa taaaaaatag taatgattag gacagattta aatttatctt
    12121 accgagttaa tttaactaac tctataatag taaataactt ttttattaca atcgaaaatc
    12181 gagaatcata taactattaa atcatatatt gctaataata aaatatgtca tctcatcact
    12241 caggggctta tcaatgtgaa gcatgtgact tggattttag ttctgaaaaa gaatttgagg
    12301 aacatagaaa aaaagaacat agcagtgaca ctaccagtaa tagttaatat gctcctggcg
    12361 ttctagaagt ataatcatct ttatccctaa tacgcaagtc atagtgggca catagaagaa
    12421 tagatttgtt gtaataccca tcttttttag aaataatatt ttttaaatta gattcatttg
    12481 tactaatttt ctttgtaatg gttatatgaa acatctttgt aacctataca ctcaaattca
    12541 ttctttaacc attctgctac ttcattaatg cattaatgtt atagtattta attattcgac
    12601 acaattttgt ttttttcttt cttattcaat aattgccata acccactaaa aatagaaaga
    12661 tgggtggagc gaacagaaat gacatcctat aattagttag gctatggcaa gagaaagagt
    12721 ttttttgtct atgcactttt tttatagtgt aatatcatat agattttaga atatttaaaa
    12781 atcggtaaac ttggttaaca atcaattctg taaatttaat tgtcatagcc tgaacaatat
    12841 aactgcaggt ccagactatg acaataattt gtttgactgc acaccttttc ctattaatcg
    12901 ttatataata tatcttcaat atcttttcga tgtacaagga aacatggttc aaacaaaagt
    12961 tatcagtgtc tacattagta gttgtaggtg ttgtttcttc tctcattatc attcttgctg
    13021 ccaatccaat acacaatgca tattcaataa atccaacaaa tcctactaat gatgtaatta
    13081 aaggatctat atccagtatt tctactgacc cttctaattc atcaacagca tggattatta
    13141 caggtgtata taaaatggaa aatgtcaaaa catctccaac atttaattct actttctaca
    13201 tgataaaaac tgatggaacg ggtaaacata gccattctat atatgatttc aaggtaaatg
    13261 cagatcctat tgtaaatact agtaataatt ctactatatt aaatgggact tccacagtaa
    13321 ccatgaagga agtaccaata aatgatgttc ccacacagat tacagtacta ggagatagtg
    13381 ctatcaatat attgttagaa ccaaccaagg tcaataatca ttttggtaat ggacctatat
    13441 atggtaatca acatttgatt tgtgtagaag ttcctagtta ttgcaaataa tagtctaccc
    13501 tcttttttct tctcttcctt ctttttctgc tatctcgatc ttctagttaa aaacgtctag
    13561 ataaactttg aataaaaaaa tactaaaaga ttgtatatga gtaatatcgc tagagtaata
    13621 gatttataaa tttataatta aataatattt gaaatattat ttattttatt ttagtagata
    13681 tatagaaact aatactcata ctcttcctcc agagtagaga tatacaaatc tataagtgat
    13741 atcgttttag acatttataa atataactat actaaaagtg agtttatttc cattcccata
    13801 ctttatagat aatattacca tcattatcta ctgaggcttc atgaacacct actaagttat
    13861 tcaaaaatga taattttcct gtttttgaag aacgatagaa agtagaacca gaaactactt
    13921 ttgatgaatc cttctgttta ctaattcctt gtgctttgac agtagccatt tcattacctc
    13981 ctttaacagt tacaatatcc ttgccttcta caaagtaggc accctctgag gttggaatag
    14041 ttgtaaacgt tcctatctcc acaacttcaa tatctctcat ttttccttct aaaaaatagg
    14101 aactttctat tgtaggtcct tccaactcta atactttttt agatgtaatt cttccttttg
    14161 cttcataaaa taaatctcct agcatatcat atgggtaaga aactaaacta ataaagtcaa
    14221 tgatataaga aattgactac ttgactaatt taaagaataa tctgatcata aaattatcat
    14281 aaatggaaaa cggcacgttc tttttgtctt cgatacgttt cttcttcttt acagattgta
    14341 aaaaatctcc atacatcaat ataactttga tacagtttct ttgataattg cacttgtatc
    14401 tttagggatt agatatgagt aattgttcat cttctattat agatatactt tacaaaacac
    14461 ctgtatatga acccagatat aatactgact cggagatgtg tcaactgatt ttgacacaat
    14521 tttgtagttc atttcggacg ggttaattta atcaaatagt tttagctata tgtatcctat
    14581 ctctagtcat cagattattt gaaagaaata aaatattcac agctatagat ctatagtaca
    14641 gttaaagcca aatttttcta tcaaatacat acaaaatgta tttaattaat attaacaaga
    14701 taaaaaatta gtaaacatgg aaactacaaa tatcaaaaaa tttgaaaaca gaactaagag
    14761 tgttattcgt ctattagctt ttttagaagc aaaaaaggga aaagagtcag aattagaaag
    14821 aattttactt gttttaattc cacctacact taacgaacct ggaaatattg cttatgttcc
    14881 acataagtca atagaaaatc ctcgggtttt tatgtttgat gaactatgga taaatgaaga
    14941 agcaatagaa gaacatttca agcaacctca tatgattaat ctattagaga aactaaaccc
    15001 gttactggaa agacctttag agttaaagag atatcatgaa ataattttgt aaattaataa
    15061 caaaaaatat cttttgtcat ctaagattaa aaattctctt tacattagtc acactcaatc
    15121 cagattctgg tcctcatcca attacataat cttgtaacaa tattttgaat ttattctcaa
    15181 ggttaacatg aaagatgtta ttgaacccta ctagtatatg ggctcggagg ggcttcgaat
    15241 tctcatcata gtggggttcg gtaacataat attaaaagtt attgattatt aaaaattact
    15301 tgttgattct tataaatttt gattaataaa ggtggtgaag atgtttatcc tagcctgtaa
    15361 gatatacaaa tttgatctat aagttgattc aacagttcca taaactagct atcctataaa
    15421 atgtttatca gtagctttat catcaaaatc tataactata gtattatcgt cacagataga
    15481 aatatctgtc tttatgttct tccattttat aacaacatct gtggatatat ccattgtacc
    15541 gattagcttg agattattat tattgcctga atgactattg cttttttttg caggagtact
    15601 aatagttgaa tcaaaagttc atgcagttac aagaatttaa gcaactccaa tcagagatat
    15661 atttggagca atattttttg tgtctgttcg tgcactcatg gataagtcat tgttattgat
    15721 atttattatt tttcctgttt ccatcttggg tagcgtttta gttgagtcac gtattagaaa
    15781 attagaccaa taaaataagc ctagagataa aaacgtaatt gtctatcaat tattaaatat
    15841 catgtcaaac aaagacatat ataaattcaa aaactaattc tatagtaatt ccatccattc
    15901 ttgtatttgt actaattgca gggataccaa cagtatttga acatgtatta gcacaagaag
    15961 gtacaatcac accagaagac tcaattaacg acacaatgtc aagtcaaaat aaaacattta
    16021 cttccagaac taatactgat tttagtgcag ttaacgcagt taacgacaca atgttaggcg
    16081 aaaatattac caatgggcta acgctacaaa atctagaaaa ccacaattaa ttttattatt
    16141 taaatggtaa aaattctgct taaatttgtt gctattattt gtttctataa atacgatttc
    16201 tatatttttt agaaattcca attaattgca aacaagtgca aataattgca agtagttact
    16261 tttaatttat atgtataccg attgcattta tataacttgc aacataaaga caacaataat
    16321 aatgtttatt aacgtaaatt ataaagaaca tttgcgatta tcgataataa tagttattgg
    16381 atttatttta gtccttcaat cattctctat tctaactgtc ctcgctcaaa ctactactca
    16441 aaacaactta actgcagaaa atctatttcc caaaattcat gaaataaaga tggctgcagt
    16501 agagttgccc gatggccata ctgcatataa aatgttagaa tacaaggtta ttgatagcga
    16561 aaatcagaca aaagatatta cctcaagata tagtgatctt ccaactatac ccggtcctac
    16621 actaatcatg actgaaggcg ataaggcaag gttaacgtta gttaatgaga ttggtcgtgg
    16681 agcagtgagt cttcacacac atggagttca ttatacaatt acaagtgatg gatcgttaaa
    16741 gatgtcaaac agagtttcag atcaaggggc aacccccaat gattcatata catatgtatg
    16801 gactgctgca gagggaacaa gaggatcatg gccttggcat gatcatacat ttggtaaagg
    16861 ttcgtctggt atgaacatga atggggtaga aacaaatggt ctcttttcta cagtaatagt
    16921 taatcccgcc aatggaaaag tagatgcttt agtaaatgga attccaaaag aggtaaatgt
    16981 gggagatata aagaaagatt ttgttttatt tgttactgat gatgcatttt ggggtgtaga
    17041 gatagacaat aacaataaca ataaacacac accattatgg gtaaatccta cactggtagc
    17101 atcaaaaaat gatttggtaa gatttcacat tcaatccgtt ggtacagact ttcatcattt
    17161 tgttattgac ggctatgaat ggctgaaacc tggaacccat gagacaacta attttgagaa
    17221 catagggcca cttgaaaatc atgtatttac tattagtgct aataataata cctcttatta
    17281 tgataaaatg cctattcatt tactttcagg aatgaaaggg aaatttcaag tgattgaagc
    17341 tgctgctggt gctactactt caagcattcc tggaccatca ccacagatgc aagatagtag
    17401 taatagtaaa gatactggta atagtaccgg tgttcctgca acatcaccaa taatgcacgg
    17461 tactagtgat aataatggag agatatcttt agtaggaagt gaataagatg agaaacaaaa
    17521 acagaagttc aaataaaaat tgtaattcga aacatacaac aataatatca tcctttaagc
    17581 cttgcttcat tctctttata atactaatag tggctactgg tcctatctct gcactaaatc
    17641 ttcctgttgt tgagatagcg tcggcacaac aacaacaaca acaacaagta tcaaaagata
    17701 cagcgaacaa tgaatctgta actttagaaa acaataagaa tataataaaa gataacaaaa
    17761 aagaagtttt tcacactttg acttcaatac cttcatcacc tgaaggcaat gatttttcat
    17821 catcatcatc atcacctaat aataaaacta acaacaatac tatgcacaag attgaaatgg
    17881 tagttattac tttaccaact ggacaaccag cttataaaat ggttagccat gttaaaagca
    17941 atggttctcc atcgtcttct
//
LOCUS       DASVEV010000171         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1038844, whole genome shotgun sequence.
ACCESSION   DASVEV010000171 DASVEV010000000
VERSION     DASVEV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038844"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..672
                     /locus_tag="VF222_07890"
     CDS             334..672
                     /locus_tag="VF222_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7142384.1"
                     /translation="MKKIEIIIPDRELQTVGAILKDNDIGGMSYYRIEGKGKGKAQPV
                     SIGRGTMQYTPEFIPRTKVEIVVRDDVVDKLVNNLLDTLDDKIGGKIFILEVQEAISI
                     RTKARGESAI"
     gene            complement(901..2049)
                     /locus_tag="VF222_07895"
     CDS             complement(901..2049)
                     /locus_tag="VF222_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX7142385.1"
                     /translation="MKNNIITIIIENLYIYINNRLTIILNKLNSDIRKIQFTGRSTYI
                     LSLPKKWIEEMNLKAGDHVSISRSSNNSLCITPEQEKRLQDSTNEVSTQVLIDEGPNT
                     LKRKIVSMYLSGYNLINLRAKSARIQPIQREAVREVVRRSLIGTEIIADSSDIITIQV
                     LLTLPELSVNTAVRRMFLIASSMHRDALVAVKEKNHDIAMGVIRSDDEVDRFSLYILR
                     NLVMATNNERVLQEIGLKNASDCLSYRVTVKSIERVADHASRIASKSMEIQSLIPSLI
                     TEKLEKLSNSALEVLTSAVEAFLRRDYNLADKIADKSDNVLCIEREIMLFLDSLENKD
                     IDSHNINAGVKLILEDIRRTVEHASDIAESAMNQTVGEIIKVEKKVEN"
     gene            2159..2368
                     /locus_tag="VF222_07900"
     CDS             2159..2368
                     /locus_tag="VF222_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142386.1"
                     /translation="MTENSVRHIDTIKELESLKYEYQIRCSHYSLEYSSKDNIITCSI
                     CGKIWNFVEKKGFSNLIQVYNAELD"
     gene            complement(2365..2643)
                     /locus_tag="VF222_07905"
     CDS             complement(2365..2643)
                     /locus_tag="VF222_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142387.1"
                     /translation="MDREGEEEMNFLDLKFFILFMWILVMIACIFIIFVIAPISKFQI
                     DQNDSLSFLKTSFIQATLAVIIVGILIVVLNKFKKVYLLKKLSARKKS"
BASE COUNT          936 a          426 c          378 g         1039 t
ORIGIN      
        1 tgtagcccca ccgtttgctg cttctagtat taagacagtt atttctgttc tacttctata
       61 tttcattatt atcaaataga acattgttga tcaagtattt attaaaagct gtccattcga
      121 tcatgtcttt cgatcagaac attcgatcta cttcgcttac tactttagaa tatactttta
      181 aaatttatat agcgatatgt acataatata gttaaaagat tttataaata ttattttttt
      241 taattatttt atgatactat ttcactgcaa tgcagtgaac acattgatgt tttaaatatg
      301 ttatataaaa aataggatgg ttgatcaata atagtgaaaa agattgaaat aataattcca
      361 gacagagaac tacaaactgt tggtgccatt ttaaaggata atgacatagg aggaatgagc
      421 tactatcgta tagagggaaa aggtaaaggt aaagcacaac ctgtttcaat tgggagaggt
      481 actatgcaat acacgcccga atttattcct agaaccaaag ttgaaatagt tgtaagagat
      541 gatgtggtgg ataaactagt caataactta ttggatacac ttgatgataa aataggtggt
      601 aaaattttca tattagaagt gcaggaggca ataagcataa gaacaaaggc taggggcgaa
      661 tctgccattt aatattttta atattttaat agcattttga ttcaaatcca ttatctaatt
      721 aatattatta tttattcata atacttataa taattattat tttcttaact acatgaataa
      781 acaagtttgg tgttttgtat gtaattcgtt aaaatccatt tgtatccatg ataattgcaa
      841 tatacactgt agtctttgtt ttaagataaa tcaaatatta ctacaaaaca atatctaatt
      901 tcaattttct acctttttct ctactttaat tatctctcct acagtttgat tcatggctga
      961 ctctgcgata tcacttgcat gttcgacagt tctgcgtata tcttcaagta ttaattttac
     1021 tcctgcattg atattgtgac tatcaatatc cttattctct aatgaatcta agaacaacat
     1081 gatctctctt tctatacaaa gaacgttatc tgatttatct gcaattttat cggccaaatt
     1141 atagtctctt cttaagaatg cctcaactgc agatgtcaaa acctctaaag cagaattact
     1201 taatttctct aatttttcgg ttatcaaaga agggattaaa ctttgaattt ccatggattt
     1261 tgaagcaatt ctggatgcat ggtcggctac tctttcgata ctttttacag taactctata
     1321 gctaagacaa tcagaagcat tttttaaccc tatttcctgc aatactcttt cattgttagt
     1381 agccatgact aaatttctta atatgtagag actaaatcta tctacttcat catccgatcg
     1441 tattactccc atcgctatat catgattttt ttcttttact gccaccaaag catccctgtg
     1501 cattgatgaa gcaatcaaaa acatccttcg tacagccgtg ttaactgata attccggtaa
     1561 tgttagtaat acttgaatgg taattatatc agaggaatca gcaataattt ctgttcctat
     1621 caaacttctt cttactactt ctctaaccgc ctccctttgt attggttgaa tccttgcaga
     1681 tttcgctctt agatttatca ggttatatcc tgacaaatac atagaaacaa tctttctctt
     1741 tagggtgttg gggccttcat caatcaacac ctgagtgcta acttcatttg tagaatcttg
     1801 tagtctcttc tcttgttctg gggtaataca taatgaatta tttgaactcc ttgagataga
     1861 aacatgatca cctgctttta gattcatttc ttcaatccat tttttaggta acgacaaaat
     1921 ataggtagat ctacccgtaa attgaatttt tctaatgtcg gaattcaatt tattcaatat
     1981 tattgttaat ctattattga tatatatata tagattctca ataattatgg ttattatatt
     2041 atttttcatt agcaatttta tatctatatg aataagtcaa taaatcacat atattttttg
     2101 taactatatt tatttctata attaatagat tatattatgt taaatctaaa taataatatt
     2161 gacagaaaat tctgtcagac atatagacac gattaaagaa ttagagtcac ttaaatatga
     2221 atatcaaatt cgttgtagtc attatagctt ggagtattcg tctaaagaca atattataac
     2281 atgttcaatt tgtggaaaga tttggaattt tgtagagaaa aaaggattct ctaacttaat
     2341 ccaagtttat aatgcagaat tagactaact ttttttccta gccgaaagtt tttttaacaa
     2401 atatactttc ttaaatttat tcagtacaac tattaaaatt ccaactataa taactgccaa
     2461 tgttgcttga ataaatgaag tctttagaaa agatagagaa tcattttgat ctatttgaaa
     2521 tttgctgatt ggagctataa caaaaataat aaaaatacat gctatcatga ccagtatcca
     2581 cataaataag ataaagaatt tgagatctag aaaattcatt tcttcttctc cttctctatc
     2641 cactggatat aataaattgc aataatattg ttattagtgc aagtgatgat gaaaagctag
     2701 ccaatttata gattttcatt actatttctc tttcagaaag ttttccatct aataaaatta
     2761 atctaactag agtgattgg
//
LOCUS       DASVEV010000172         6606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1625599, whole genome shotgun sequence.
ACCESSION   DASVEV010000172 DASVEV010000000
VERSION     DASVEV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625599"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1530
                     /locus_tag="VF222_07910"
     CDS             379..1530
                     /locus_tag="VF222_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7142388.1"
                     /translation="MNNNNNNNNSWLLFRGNEARSGIFPEKLTRELSLFWVIELGPMI
                     SSAISHNNIVYNSTITGRIFAIDVYKKKINWQKEVGSPLVSSLLLYDNLLISCTFNSW
                     ITNANSKTPERRNFIYAFDINNEGKKKWNVQITGDIFSSPCICDGNIIVIGSLDSTIY
                     AIDMRGNIIWTFNTGNSIWSSPATNNHEIFIGSDDCCLYSCDLDGNLNWKSMLEGKIR
                     ATPSLMKSTNSLFIGTHSGIMYCLDQSDGSIKWKYETGKPILSSVSISKDHIFFGCSD
                     KKIYSLNSVDGSINWKYETEDKIWSSPVIAQNNNDDEEGILYVGSLDSHIYGLGIESG
                     KLNWKFPTMDGIESSPCIVKNKLFISSKDGLLYCFSNQQNDLNLMRKLF"
     gene            2037..2429
                     /locus_tag="VF222_07915"
     CDS             2037..2429
                     /locus_tag="VF222_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142389.1"
                     /translation="MTNPIIAKNSRRLNPLYIVALAMVATLLAFIPSLSQLSYAEDYK
                     LNININNQENADIKTDVSIKTDEGKEKTEIKLNDNTEKIKETITGEPGEKVKVCLEES
                     DDNKKYCQTKKLPQDEDSIVKFKLEYET"
     gene            complement(2682..3647)
                     /locus_tag="VF222_07920"
     CDS             complement(2682..3647)
                     /locus_tag="VF222_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7142390.1"
                     /translation="MRILVTGAGGFIGSNLVSRLSFATKDTEIVCMTRNAEKLKNKIS
                     KNSNVKIAEADVMDYVQIKDTLTKIDIAYYLIHSMEGVSAKEWKKFAERDRKAAENFA
                     KAATECKVKRIIYLGGLIHEDKNTTTNSKRGQIKISEHMQSRIEVGNILKKSLAHVTI
                     FRAAVILGKDGASYKMLRYLVERLPVMVCPKWVLTNSQPIAIDDVITYLVKSIEKEET
                     EGKEFDIGGPDTLRYIDMMRRYAKVINKKFLKIVIIPFLTPKLSSYWVELITPTKASL
                     VRPLIESLKYEAVVKDDSIKKIIPIKLKTFEEAIIAAKAKEDEDK"
     gene            4138..4422
                     /locus_tag="VF222_07925"
     CDS             4138..4422
                     /locus_tag="VF222_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142391.1"
                     /translation="METKKSKNNDNKDNEKKEIEDNNNIHPSQYTFDSSDAKEIMTGV
                     GGGEGSAYPDSDKHIVEDIKKVIKEEKTGLGEEGYAKSDSEQSNNKDENN"
     gene            complement(4587..4748)
                     /locus_tag="VF222_07930"
     CDS             complement(4587..4748)
                     /locus_tag="VF222_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142392.1"
                     /translation="MSLCNKKYYNFVIVGTLESESKDTALEFLEKLMRVEKINGLSAL
                     HIFGENIPK"
     gene            complement(5101..5862)
                     /locus_tag="VF222_07935"
     CDS             complement(5101..5862)
                     /locus_tag="VF222_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7142393.1"
                     /translation="MKRVKKSVNFNVNFASTMLNNIIFSNKYSSFDFSKIFYFSLLSI
                     LASLVIPLQINGGEQQEHNIIHLVWAAEINGTNNADNISGTINKDIIKGLDGNDTISG
                     KEAGDDISGGSGDDIIYGNEGRDVLKGKAGNDHIEGEEGKDRIFGDRGKDVLVGGQDN
                     DTLNGGLGKDTFICGDGTDILIEFNITQKDITPENDCENQKNGNGKTKSLSLQQDSNS
                     AVTMDGNDITDNKSNNVKTTNENKKNDDFFFGLFK"
     gene            6370..>6606
                     /locus_tag="VF222_07940"
     CDS             6370..>6606
                     /locus_tag="VF222_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142394.1"
                     /translation="MVFVITVETLPAFITMKVILQQGSVDLDRKKIIIFFNNDFIYGY
                     FWNFNILLKNLLKQYTSMSIEANNYLYTYMYCKNI"
BASE COUNT         2328 a          949 c          866 g         2463 t
ORIGIN      
        1 agctatttca gttttaccag ttccagtgct atatactatt gtacctctaa aatcattatc
       61 aatccatgcg tttactgcat cgatttgatc ttccttgagt gaaaaaggga attttagttg
      121 agaaatggcg gcggcggttg aatcagacat aatgcattta ctattataat atagtattga
      181 taatcattca tttcatataa atcaataacc aataattcgt tttatttttg gatattgtga
      241 gaattatatg gactacaatg taatattgat atcaataaaa aataatttat ttcacaagca
      301 ttttagtgtt tattactcgg taccttttat gattacgaca taaaataaat tatagatcac
      361 aattttttat tctattactt gaataataat aataataata ataattcttg gctcttattt
      421 agaggaaatg aagctagaag tggaattttt ccagaaaaac taacacgaga actatcgcta
      481 ttttgggtaa ttgaattagg acccatgata tcctctgcta tttcccataa taacattgtc
      541 tataattcga ctataactgg tagaattttt gctattgatg tttacaagaa aaaaatcaat
      601 tggcaaaaag aggtaggatc tcctttagta tcatcattat tactttatga taatttgtta
      661 atatcatgca cttttaattc atggattact aatgcaaatt ctaaaactcc tgaaaggagg
      721 aattttattt atgcattcga tatcaataat gaaggcaaaa agaaatggaa cgtacaaata
      781 actggtgata ttttttcatc tccttgtata tgtgatggaa atataatagt gattggatcc
      841 cttgattcta caatttatgc aatagacatg agaggaaata taatatggac atttaataca
      901 ggtaattcta tttggtcttc cccggctacc aataatcatg aaatatttat tggttcagat
      961 gattgttgtc tctatagttg tgatttggat ggtaacttaa attggaagag tatgttagaa
     1021 ggcaaaatta gagctacacc aagtttaatg aaatcgacta attcgttatt tataggaaca
     1081 catagcggta taatgtattg tttagatcaa tcagatggtt ctatcaaatg gaaatatgaa
     1141 actggtaagc ctattctttc ttcagtatct atatcaaaag atcatatttt ctttgggtgt
     1201 tctgataaaa agatctattc attaaactct gttgatggtt ctataaactg gaaatatgaa
     1261 actgaagaca agatttggtc ttcaccagtg atagcacaaa ataataatga tgatgaagag
     1321 ggaatattat atgtgggttc cttagattct catatatatg gtttaggtat tgaaagcggt
     1381 aaattaaact ggaaatttcc tacaatggat ggaatagaat catctccttg tattgtgaaa
     1441 aataaactgt ttattagctc aaaagatggt ttattatatt gttttagtaa tcaacaaaat
     1501 gatcttaact taatgagaaa attattctga atgtaccatt cagaaatatg tgctaagatt
     1561 tgctaaaaat agaaattccc acttattgtg ttgttttatt cattcgttct ttctctctct
     1621 ccatctcttt tttgtgtatg ttgttgtgtg actaatcaaa gaagcatttg gtgaaaggag
     1681 aaataaatca aaaataagaa aaagatggat aaaccacttt tctagtaatt atgaaaagaa
     1741 tgaaaaaact tgctaaatat tgattgttag aatttgtata ttaaatcagt tagattttat
     1801 gacgacgatc taacactaaa tcatatgaag agtagataga ttatgttttc ttgaccaatc
     1861 gtctttatat taatacgcat attatttttt aaaataattc taatctgcaa aaagaagaat
     1921 agtttagtat aaatattata taaaaacaaa cataattctt ttcaatatct ttttttatat
     1981 ttattctata taaaactatt aaaaaatttt tataatttaa tttgttaata agaaatatga
     2041 ctaatcctat tatagcaaaa aactctagga gactgaatcc tctatatatt gtagctttag
     2101 caatggtagc cactttattg gcttttattc cttcattgtc tcaactgtct tatgcagaag
     2161 attataaact taatataaat atcaataatc aagaaaatgc tgatattaaa acagacgtct
     2221 caatcaaaac agatgaaggt aaagagaaga ctgaaataaa attaaatgat aatacagaaa
     2281 aaataaaaga aacaataact ggagaacctg gagaaaaagt taaagtatgt ttagaagaat
     2341 cagatgataa taaaaaatat tgccaaacta aaaaattacc acaagatgaa gatagtattg
     2401 tcaaattcaa gttagagtat gaaacttgaa ataaagaata ataataataa taattactaa
     2461 atattttttc tatttaactc taataattat tttttaactc aatatttcgt tcgttagctt
     2521 ataataaaag aaaaattttt ttctatttat tatcaataca tatagttacc tgccaaatcc
     2581 atatcaaatc agaagtagaa aaagttattg tatagcagac cccttaatcg ttaaaatttc
     2641 tccattttta tagctctatg aagtagcact aattttcatt tctatttgtc ttcatcttcc
     2701 ttagcttttg ctgctataat tgcttcttca aaggttttaa gtttgatagg aattattttt
     2761 tttattgaat catcttttac tactgcttca tatttaagac tttcaataag tggccttaca
     2821 agagatgctt tcgtaggagt aataagctcg acccaataag atgataattt tggtgtaaga
     2881 aaaggaatta ttactatttt taaaaatttt ttatttatta ccttagcata gcgtctcatc
     2941 atatctatat atctaagtgt atctggaccg cctatatcaa attcctttcc ttctgtttct
     3001 tctttttcta ttgatttgac taaatatgta attacatcgt ctattgcaat aggctgagag
     3061 tttgttaaaa cccatttagg acacaccata acaggcagtc tctctacaag atatcttagc
     3121 attttatacg atgcaccatc tttcccaagt atcacagcag ctcgaaagat agtaacatgt
     3181 gctaaggatt ttttcagtat atttccaact tctattctac tttgcatatg ctcagatatt
     3241 ttgatttgcc ctcttttact atttgttgtt gtattcttgt cttcatggat taatcctcct
     3301 aggtatatta ttctctttac tttacattct gttgcagctt tagcaaaatt ttctgctgcc
     3361 tttctatctc tttctgcaaa ctttttccat tcttttgcag aaactccttc cattgaatgt
     3421 attagataat acgcaatatc tatttttgtt agtgtgtcct taatctgtac atagtccatt
     3481 acatctgcct ccgcaatctt gacattgctg tttttgctta tcttattctt taatttctct
     3541 gcatttcttg tcatacaaac aatttctgta tcttttgttg caaaggataa tcttgacact
     3601 aaattacttc caataaatcc tcctgctcca gttaccaaaa ttctcatcta tttataaaaa
     3661 acatatctac gatattgcat atatgtatag atcatattat attgtatatg atagaaaaga
     3721 tagaagaata gacacaactt aataattcct tagcattacc atattagcat acaaaggtaa
     3781 tgcaaaattt tcattataac tctattcatt ctactatgtc ctccataatc atgcgatcct
     3841 gctgttttat ggaagaagaa aggagcacta aatttacatt tgagaatcgg ttaaactttt
     3901 cataaaccta tataataaat attgttaaca atttcaagat gtatctctaa acttatattc
     3961 caacgtttat tatatcatgt aatgtaatat attataacaa cttttgataa tcaattatct
     4021 caaattacat tagtaaatta acagaatgaa cagtatctct taatttccac tactcggata
     4081 ataattatca ttttaaagca aaaaaattta aaactgttat tactatcaaa agataatttg
     4141 gaaactaaaa agtcaaagaa taacgataac aaagataatg aaaagaagga aatagaagat
     4201 aataacaata ttcaccctag tcagtatacg tttgattcat cggacgctaa agaaatcatg
     4261 acaggtgtag ggggaggaga aggtagtgct tatcctgatt ctgataaaca cattgtggaa
     4321 gacatcaaga aagttatcaa agaggaaaaa actggactcg gtgaagaagg ttatgctaaa
     4381 agcgattcag aacaatcaaa taataaagat gaaaataatt gacttgttta tctttgccaa
     4441 tagggaatta ttgactaaga tacagtaaaa aaaggacaaa aaataattaa atttctgtat
     4501 agagattagc taaaaagagc ctgagaatcc ttacctataa ctcatctagg gtaacgatgg
     4561 aaattaaata attttttatt attagattac tttggaatat tttctccaaa aatatgaaga
     4621 gcacttaaac cattaatctt ttcaactctc attaattttt cgagaaattc taaggccgta
     4681 tccttagatt cactttccaa tgttcctaca atcacaaaat tataatattt cttattacat
     4741 aggctcattc taaaccttta acttttgata tcgatatata taagattatt gcaatgaata
     4801 gagtataaaa gttacaattt attataaatt ttttcattta tcattatatt tgtttagtat
     4861 tcattcattt ctttaaatct tcaagtgaaa attactacta tccaactcct ttgatattta
     4921 cgtttttctt ggaaatagtt acttccgaaa attcgagtct gctactgttt gattttaatt
     4981 attagtgagt tgagtattat ttttagtatg tatcaccata tagtaataga tttataatat
     5041 ctattactag gcattaaaag aagttaattg taattgttta tttcatttat cttctttgta
     5101 ttatttgaat aaaccaaaaa agaagtcatc gtttttctta ttctcatttg ttgtttttac
     5161 attattactt ttattatcag ttatatcatt gccatccatg gttactgctg aatttgaatc
     5221 ttgttgtagt gataaacttt tagtttttcc attaccattt ttttgatttt cacaatcatt
     5281 ttctggagtg atatcctttt gtgtgatatt aaattctatt agtatgtctg ttccatctcc
     5341 acaaataaat gtgtcctttc caaggcctcc attcagtgta tcattatctt gcccgcctac
     5401 tagtacatct tttcctctat caccaaatat tctatccttt ccttcttcac cttctatatg
     5461 atcatttcct gcttttccct ttaggacatc tcttccttca ttaccataaa taatatcatc
     5521 tccacttccg ccactgatat catctcctgc ttctttgcca cttattgtat cattgccatc
     5581 caatccttta atgatatctt tgtttatcgt accactaata ttatctgcat tgttagttcc
     5641 attaatctcc gctgcccata ctaaatggat gatattatgt tcctgttgtt ctcctccgtt
     5701 aatttgtaat ggtataacta gtgaagctaa aatagaaagt aaagaaaaat aaaaaatttt
     5761 agagaaatcg aaactactat atttatttga aaaaataata ttatttaaca tagtactagc
     5821 aaaattaacg ttaaaattaa cagatttttt tactcttttc atttgtgata caataatgag
     5881 tataaataat ttaaattatt gcaaaacctt tctataacaa tgatacatta ttatttataa
     5941 taataatgtc attaaattta attaattcgg tatatataaa caatatattt ttctcaagta
     6001 tttctcgtca cttataaaaa taccatagat ggatatttac attaattaat tgtaaaaata
     6061 tacaaatact taattttctg aattaatcca tttatattta agcgaatcaa gaaggttgat
     6121 tggttcctct caataaaaat acaagacttt tgaaaaaata tttaatattt ctcaaagtta
     6181 cactaataga aaaaaaggat taaattctat ggtgaacaat ctcatcgatt aagagaatat
     6241 ataggaaaat tgattgtttc aaaatcaaca ctaataccaa gtaaaacaat ttattaataa
     6301 agtaaatgag atgttttaat tgttatattg agttttctcc gacattagta aagccaatat
     6361 gtccatcttg tggtttttgt tattactgta gagactttgc ctgctttcat cacgatgaag
     6421 gtgatactac aacaaggaag tgtagattta gatagaaaaa agataattat tttctttaat
     6481 aacgacttta tttatggata tttctggaat tttaatatac ttctgaaaaa tctgttgaaa
     6541 cagtacacgt caatgagcat cgaagcaaat aattatctct atacatatat gtattgtaaa
     6601 aacatt
//
LOCUS       DASVEV010000173         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_461044, whole genome shotgun sequence.
ACCESSION   DASVEV010000173 DASVEV010000000
VERSION     DASVEV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461044"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..442)
                     /locus_tag="VF222_07945"
     CDS             complement(83..442)
                     /locus_tag="VF222_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142395.1"
                     /translation="MKKLNNNVFFFYDVTKCNIHEFFFDWNCNDCKEEYRDLKGTNKI
                     DEESHEEVEPIKTKKIKKMNDYKEFSEEKAKKLYEEIFLQYLKKDQITEAEAIERSKR
                     IIRKQCLMRNIEPWSWV"
     gene            489..562
                     /locus_tag="VF222_07950"
     tRNA            489..562
                     /locus_tag="VF222_07950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Thr,seq:cgt)
     gene            complement(585..968)
                     /locus_tag="VF222_07955"
     CDS             complement(585..968)
                     /locus_tag="VF222_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HEX7142396.1"
                     /translation="MPIISISLNEHIIQELDKLQKFLGFSGRSEIVRASVRNLLLEEK
                     RIDELSGVLHSVLLVIHDEKSDQEISEIRHGFDKIINTHIHNKIDKDRCLEIFVLYGD
                     AREIKNITKRFQGNRKMDQVRLVVT"
     gene            complement(1047..1913)
                     /locus_tag="VF222_07960"
     CDS             complement(1047..1913)
                     /locus_tag="VF222_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142397.1"
                     /translation="MNFGIKFVFVFLFASSLLFFTCLFIFDIITVFAQQENTTINAND
                     QFGIEKIYPTKSDGREWYVNMSNPKNDPNVTFTFNPRLAKQDDGSWKIRDDKIRINVD
                     TLNGTEEWKNIEITGYVKIDSIINPRKSVITDIDWVARTDQHNEDFPCHGLSLHGGIY
                     PDGSAAWKKEIWFTGGYTKEKSVPKITDSLMNRWIGWKTVIYNTNNDSAVKMESYLDN
                     SNSNHWVKVFEITDNGGWYASSSDKVFYSANCGRSKDHMVLNSGPIVTFRADNIVLNF
                     KDLSIREIEPPK"
     gene            complement(1985..2824)
                     /locus_tag="VF222_07965"
     CDS             complement(1985..2824)
                     /locus_tag="VF222_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7142398.1"
                     /translation="MIEILQYEFMQRALIAGIAISISCSLIGLFLILKRFSLFGDAMS
                     HVAFGGIALGLFLKSNPIWVSLIVSIIGALAIIKLNSSKRIYSDSSISVLLSLGLAMG
                     LVLISLSGGFSIDITSYLFGSILLVNIEETLSTILLSVIVIAFVILFYKKLVYLVFNE
                     EQALVNGINTVVLNILFITLATIAIVMSIRLIGVLMVSSLLIIPNVSSLLLGYGFKKT
                     ILLSICFSLTSVILGIILAYEWNITPSGMIVITAAGIFFGVNVLKLFSSKIKSKSKTK
                     ITS"
     gene            complement(2867..>3765)
                     /locus_tag="VF222_07970"
     CDS             complement(2867..>3765)
                     /locus_tag="VF222_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7142399.1"
                     /translation="SSIIPKSIATSNLSQNSNNSINESEGQGLISSNNTKLKIFASFY
                     PIYDFVKKIGKDRVDVSTIVPASIEPHDFEPTAKQVIELQKADVIFINGAGFESWINK
                     IGDAAVVDLSKDLPIENLGSTPNPHIWLDPNLVKAYSKTIFEKLISLDPQNTDYYADN
                     FNEFNNKLELLNLDISKNLTNCDLNDFIAFHDAFGYFAKRYGLTQHSISGSSPEADIN
                     PQRIADAIKLAEQLGVNIIFSEDNIEPRLSNTIANEVGGKVLILSPIEMVTLEEQVLN
                     KDYFSKMYENLDNLKIALRCEN"
BASE COUNT         1210 a          657 c          502 g         1396 t
ORIGIN      
        1 gattgttcgg tgatgacaat aaatttaaat aacttttttt tgttatttag gtttaactta
       61 actatttaat tctgaatctg gattaaaccc atgaccaagg ctcaatattg cgcattaaac
      121 attgttttct tattattctc tttgagcgct caatagcttc tgcttcggtt atttgatctt
      181 tttttaaata ctgcaaaaaa atttcttcat agagtttctt cgctttttct tcgctaaatt
      241 ctttgtaatc attcatcttc tttattttct ttgtcttgat gggttctact tcctcgtgtg
      301 actcttcatc aatcttatta gttcccttca aatccctata ttcttctttg caatcattac
      361 aattccagtc aaaaaaaaat tcatgtatgt tgcattttgt cacatcatag aaaaaaaata
      421 cattattatt taactttttc atacttctaa tacttgtaaa ttatttgtat accggttcta
      481 atgctgccgc catagtagct cagcctggca gagcgaaggt ttcgtaaacc ttaggccgtg
      541 ggttcaaatc ccacttatgg ctttcaaatt aatatattga attattatgt taccactaac
      601 ctaacttgat ccatttttct gttcccttga aatctcttag taatattttt aatttctctt
      661 gcatcaccat atagcacaaa aatttcaaga cacctgtctt tgtctatctt attgtgaatg
      721 tgggtattaa ttattttatc aaatccatgt cgaatttcac taatttcttg atcagatttt
      781 tcatcatgta ttactaaaag aactgaatgt agaactcctg acaattcatc tattcttttc
      841 tcttctagaa gtaagtttct gacactagct cttacaattt ctgatctgcc tgaaaaaccc
      901 aaaaactttt gcaatttgtc tagttcttga attatgtgtt catttagtga aatgcttatg
      961 attggcaatg ttttttatta tgatcatttc ataatatttg ttttttccat ttatgaataa
     1021 tttccaatag ataaatcagt atcatcctac tttggtggtt ctatttctct tatgctcaaa
     1081 tctttgaagt ttaaaactat attatctgcg cggaaagtaa caataggtcc tgaattcaaa
     1141 accatatgat ccttggatct accacaattt gcactgtaga atactttatc tgaactactt
     1201 gcataccagc ctccattatc cgtaatttca aaaactttga cccaatgatt agaattggag
     1261 ttatctaaat atgattccat ttttaccgca gaatcgttat ttgtattata gatcacagtt
     1321 ttccaaccta tccatctgtt catcaacgag tctgttattt taggtactga tttttcttta
     1381 gtatatcctc cggtaaacca tatttctttt ttccaggctg ctgaaccatc cggataaatt
     1441 cccccgtgaa gggataggcc gtgacaagga aaatcttcat tatgttgatc agttcttgct
     1501 acccagtcta tatctgttat aacactttta cgcggattga ttattgagtc tattttcaca
     1561 tatccagtaa tttcaatatt tttccattcc tctgtcccat ttaatgtgtc cacattaatt
     1621 cgaattttgt catcccttat tttccaagat ccatcatcct gtttggctaa tctaggatta
     1681 aaagtaaaag ttacatttgg atcatttttg ggattagaca tattaacata ccattccctg
     1741 ccgtcggatt tggtaggata tattttttca ataccaaatt gatcatttgc atttattgtg
     1801 gtattttcct gttgagcaaa gactgtgata atgtcaaaaa taaataaaca agtaaaaaac
     1861 aaaagtgatg aagcaaaaag aaagacaaat acaaatttta taccaaaatt catttcttta
     1921 aaaacatact ggttaaacaa gtacataata atatcgggca atagatgata aaaactatta
     1981 attatcaaga tgttattttc gttttagact tgctttttat ttttgaagaa aataatttta
     2041 atacatttac tccaaagaat attcccgcgg ctgttattac tatcattcca ctaggtgtaa
     2101 tattccattc atatgctagt attataccta aaattacaga tgtcaaagaa aaacaaattg
     2161 ataatagtat agttttcttg aatccatatc ctagtagcaa cgaagaaaca ttaggaatta
     2221 ttaagagaga tgaaaccata agtactccta tcaatctaat ggacattaca attgcaatag
     2281 ttgccaaagt aatgaataaa atatttaata caactgtatt gatgccattt actaatgctt
     2341 gttcctcatt aaatacaaga tatactaatt ttttataaaa taggataaca aatgcaataa
     2401 ctatgacgct taaaagtatg gtactcaatg tttcttcgat attaactaat aatatgcttc
     2461 caaaaaggta acttgttata tctatagaaa atcctcctga taagctgatt aataccaatc
     2521 ccatcgctag ccctagagat aatagaacag agatagacga atctgaatag attcttttgc
     2581 ttgaattcaa ttttatgatt gctaatgctc caattattga aacaattaat gatacccaaa
     2641 taggattaga tttcagaaac aatcctaatg caattcctcc aaaagccaca tgcgacattg
     2701 catcaccaaa caaagaaaat ctttttaata taagaaacaa tccaatcagc gaacaactta
     2761 tagaaattgc tattccagca ataagggcac gttgcatgaa ctcatactgg agaatttcaa
     2821 tcattactat tcaattcttt tttcatattc tatataattc aaataattaa ttttcacatc
     2881 tgagcgctat tttcaaatta tccaaattct cgtacatttt tgagaaatag tctttattta
     2941 aaacttgttc ctccagtgta accatctcta taggactcaa tattagtact ttccctccta
     3001 cttcatttgc tattgtgttt gaaagtctag gctcaatatt atcttctgaa aaaataatgt
     3061 ttacacctag ttgttcagct aactttattg catctgcaat tctttgaggg ttaatgtctg
     3121 cttcaggtga cgaaccacta attgaatgct gagttaaccc gtatcttttt gcaaaatagc
     3181 caaaagcatc atgaaatgcg ataaaatcat tcagatcaca attagtcaag ttcttactaa
     3241 tatctaaatt taatagttct agtttattat taaattcatt aaaattatct gcataataat
     3301 ctgtattttg aggatctaaa gatattaatt tttcaaatat ggtttttgaa tatgctttaa
     3361 ccaaattggg atcaagccaa atatggggat ttggagtaga tcctaaattt tcaatcggga
     3421 gatccttgct caagtcaact acagccgcat ctcctatctt attaatccag gattcaaatc
     3481 cggctccatt aataaaaatt acatcagctt tttgtagttc aattacttgt tttgctgtag
     3541 gttcaaaatc atggggttct atactggcag gtactatagt tgaaacatcc actctgtctt
     3601 tgcctatttt cttgacgaaa tcataaatcg gataaaagga tgcgaaaatt tttagctttg
     3661 tattatttga tgaaattaag ccctgaccct ctgattcatt gatactatta ttactatttt
     3721 gtgataaatt gctggtagca atagactttg gaataattga agaaa
//
LOCUS       DASVEV010000174         9382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1626129, whole genome shotgun sequence.
ACCESSION   DASVEV010000174 DASVEV010000000
VERSION     DASVEV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626129"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF222_07975"
     CDS             <1..376
                     /locus_tag="VF222_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7142400.1"
                     /translation="VEFNQIEGVVTGKRITLRDLMGIQTEFYKKIGITKIKFWPTFFP
                     YTEPSLQSMIFNDNLNKWIELFGMGILRPEVVTPLGIRNIVLAWGGGIERIAMLKYGI
                     SDVRELYNNKIGRLRDLSKCQL"
     gene            364..2034
                     /gene="pheT"
                     /locus_tag="VF222_07980"
     CDS             364..2034
                     /gene="pheT"
                     /locus_tag="VF222_07980"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7142401.1"
                     /translation="MPVINILKPRIRSAFPNKKIDEIIEKLPFLGLDIEGIDKEKIRV
                     EYNPNRPDFSSDTGIFRSLKGLLNETVGLPLFKYSSDKSYQITIDPQVKQIRPIILFL
                     VAKNKTVNNFILNQLISVQEDLHNGIARGRKKAAIGIHDLDAIKFPLLYSTRSQNESF
                     IPLDQIKPFKFKQVLSDLEVGKKYSNLLKNFIQYPTIADKSNEVVSFPPIINSSNTKI
                     TGKSHNILIEITGTNLETCKSILAILAYYLHDEGYEIFSGIVHDGKSTFSSLKLLKNN
                     VVSVDKNIIKKYLGLDLLIKDFIEILNRCRLDVSITKNKRIKCLVPSYRSDIINPIDI
                     VEEIAIGYGIDNLKSTLPAFQISSGVRNDHFRKIDEIKETLIGHGFLEMVNFSMLDRT
                     ISTIFNFDDKELLEKIKVSESKSKEHEFLRTNILSSLVNNLSYNVHEEYPQKIFEIGK
                     TFSFKNKIVEQWNLGVLLASKNSNYTAAKSILESLFKINFGKEFTTRRTDNNLFIKGR
                     SANILYESKIIGFIGEISPDLITKFNLRIPLSAFELNLSTFFQSCMVK"
     gene            complement(2362..2538)
                     /locus_tag="VF222_07985"
     CDS             complement(2362..2538)
                     /locus_tag="VF222_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142402.1"
                     /translation="MISRQKEAEIISDILLRAASEPEFRNLLIKNPGDILEKYNISNE
                     AKYIIKKSIVDLTQ"
     gene            2632..3930
                     /locus_tag="VF222_07990"
     CDS             2632..3930
                     /locus_tag="VF222_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX7142403.1"
                     /translation="MRSQKKWTLNGTSRVIPVEETLLRVSEISKEIGLTRIADITYMD
                     KLYIPNYSCVLPGTEDYIWVYSGKGPTKQHAKVSAIMESIERYSSLPTYYHSKFISGT
                     FNELKQSYNILHPDEVIEPLTFDFQNDMLMDYVKGYDIINKEDILVPAALALFRFTPH
                     SPSVNPFAFHHTNGLASGNVFEEAVCHALCEVIERDAISLAQLRASAIPFHILYSIYN
                     KFKLQGYHLDAFTKHDFKDDNSLYHDVLISEEVISPISKLIKKFKKFNIDLIIKDITT
                     DIKIPTFNVACVEWISHDYGYLAEGHGTHPDKRIALLRAITEVSQTRAANIQGARDDL
                     RKIKYNDKNTDDKNAWQFMSSHKTIKFSDIITYQNDDILDDINLIIKFFKEIGLNKAI
                     IVNLTNPKIKIPVVRAIVPGLETFKISKSVMGFRARRSYK"
     gene            3927..4592
                     /locus_tag="VF222_07995"
     CDS             3927..4592
                     /locus_tag="VF222_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HEX7142404.1"
                     /translation="MNLPIIFLGPSLQINKAKKIINADFRPPAKKGDFINLNLLSEKR
                     EIVLIDGVFLQDYPPTPIEVFQVINNKNFEVYGASSIGALRAVELEKFGMKGIGKIFE
                     LFKKNIVNSDDEIAVTFDSNYNLLSEAMIDIRYNIFLGWRNGIIDKETKQIMIKLAKR
                     IYFPFRTYENIIKKSRHLFPSKEKYISRFNEYIVVNKVSLKELDAIRALKYISSLTKN
                     KNN"
     gene            4667..5047
                     /locus_tag="VF222_08000"
     CDS             4667..5047
                     /locus_tag="VF222_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142405.1"
                     /translation="MNRSKLNEHYKTNCKKILELSPKIRYVGITNKFGRTISGQLRTG
                     IIPFLKTEEMRNELFVHSMIYNIRKSYEKSIGETEFIFISNENVDIVSFYMDDLVYYM
                     TIDKNIDKNELYTVVEKTRGIAKS"
     gene            complement(5072..5527)
                     /locus_tag="VF222_08005"
     CDS             complement(5072..5527)
                     /locus_tag="VF222_08005"
                     /inference="COORDINATES: protein motif:HMM:NF003269.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HEX7142406.1"
                     /translation="MYRQMTKTWITMWKTNSEELKSRAVGWRKELSIHRINRPSRIDR
                     ARRLGYKAKQGIVIIRARVGRGGMRKQRPVAGRRPKHSGVVHIKQATNMKLVAERRVN
                     EKYPNMEVMGSYFLYKDGKHSWYEVILADPSHPSIYKDKEMRGKLKNLT"
     gene            complement(5633..6103)
                     /locus_tag="VF222_08010"
     CDS             complement(5633..6103)
                     /locus_tag="VF222_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7142407.1"
                     /translation="MINIDEKLYALGFELPPSPKSAGSYIPLVEVNGLIFISGQIPLD
                     TVSKPATVKYKGKIGKDTTLEKGQDAAILCTLNALALLKEHLGSLDKIKQIVKLSAYI
                     NCLESFTDHPTVINSASNLLEKLFGEKGKHSRIAIGVESLPLGSTIEIDFIISI"
     gene            6181..6561
                     /locus_tag="VF222_08015"
     CDS             6181..6561
                     /locus_tag="VF222_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142408.1"
                     /translation="MIKRVLLSLAVGVIITYLVYLGLMDSLTRSSVNYLSISNDTAIS
                     LIFKNYNLSSQHLTNINSKYVYIKGNGEIFESKKDTNFIGNYIGKTSPTITTGNHFGW
                     EVQFPALNKTYYVDHLRGDIISTK"
     gene            6642..7577
                     /locus_tag="VF222_08020"
     CDS             6642..7577
                     /locus_tag="VF222_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7142409.1"
                     /translation="MHLTGNVLVCDPISKIGITILKKAGLNVVEKPSITNSELESEVE
                     NFEVIVVRSRTKITNEIISKAKNAKIIARVGVGLDNIDVESARQNNIEVINSPESAIN
                     AVAELVVGLMLSLSRNLTLADSEMKKGNWMKKELNGIELRGKYLGIVGVGNIGRNLAR
                     IARSLRMNIIGYDTFPIKQDFINEVGMITTDFKTLVASSDFISCHVPLAPDTKHMFNE
                     ATFSNMKSTAFFINSSRGEIVDENALYSALITKKIAGAALDVFEVEPPTNIKLMQLPN
                     IICTPHIGAQTIESQDLASNVIAEKIIHRLLEKET"
     gene            complement(7593..7686)
                     /locus_tag="VF222_08025"
     tRNA            complement(join(7593..7629,7650..7686))
                     /locus_tag="VF222_08025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7650..7652),aa:Ala,seq:tgc)
     gene            8504..>9382
                     /locus_tag="VF222_08030"
     CDS             8504..>9382
                     /locus_tag="VF222_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142410.1"
                     /translation="MIKIETLLGFSSFNKNMSVFLKDRVWIMLSEISKRGVYPLHGYK
                     LGIFRLPVNLTEESTINNIYNELKKTGFKLDRYADRIYVTHIWKEQERDNLLATLLSI
                     SLDITVEIVTGEVIDLIYQIKPIEKFGDSYRINNYRQKADFNAKMVIDTIIRNTSIGD
                     KLISYYQKTEKLSLEKAIDKLTQLTPLALNPITRNVNPPPPPAGASISSQEISSSSVT
                     TTRSAPNIPNIPSATTMTSGNSDTTISLTGSGTDHSKVDGPIDTSFKSKRQKAGKFKG
                     ISVWGPVDPPGQLGIWG"
BASE COUNT         3446 a         1361 c         1452 g         3123 t
ORIGIN      
        1 tgtagagttt aaccaaattg aaggagtagt aacaggaaag cgaataacat taagagactt
       61 aatgggtata caaacagaat tctataagaa gattggaatt acaaaaatta aattttggcc
      121 aacttttttt ccatataccg aaccttcact acagtcaatg atcttcaacg ataatttaaa
      181 taaatggata gagttatttg gaatgggcat acttaggcca gaagtagtaa caccccttgg
      241 tataagaaat attgttttag catggggagg tggaattgaa aggatagcca tgttaaagta
      301 tggaatttct gatgtaagag aattatataa taataaaatt ggaaggttac gggatttaag
      361 taaatgccag ttataaatat cttgaaacca cgtattagaa gtgcttttcc aaataaaaaa
      421 attgatgaaa taattgaaaa acttccattc cttggattag atattgaagg tatagataaa
      481 gaaaaaataa gagtagaata taatcctaat cgtcctgact tttcaagtga cacgggaata
      541 tttagatcat taaagggctt actaaatgag actgtaggat tacctctttt caagtattct
      601 tctgataaat catatcaaat aactattgat ccacaagtaa agcaaataag accaataata
      661 ttgtttttgg ttgctaaaaa taagacggta aataatttta ttctaaatca attaatttca
      721 gtccaagaag atcttcataa cggaatagca agaggaagaa aaaaagcagc aataggtatc
      781 catgatcttg atgctattaa gtttccatta ctctatagta ctcgatctca aaatgaatca
      841 tttataccat tggatcaaat taaacccttt aaatttaaac aagtattatc tgatttagag
      901 gttggaaaaa aatatagtaa tttattaaaa aatttcattc aatatcctac tatcgctgat
      961 aaaagtaatg aagtagtatc ttttcctcca atcataaata gtagcaatac caaaattaca
     1021 ggcaaaagtc ataatatact aatagaaata acaggtacaa atttagaaac ttgtaagagc
     1081 attttagcaa tacttgctta ttatctccat gatgaaggct atgaaatttt ttccggaatt
     1141 gtacatgacg gtaaatcaac attttcttcc ttgaaacttc ttaaaaataa tgtcgtttca
     1201 gttgataaaa atattatcaa aaaatattta ggattggacc tattaattaa agattttata
     1261 gagattttaa atcgatgtcg attagatgtt tcaataacaa agaataaaag aattaaatgt
     1321 ttagtacctt cgtacaggag cgatataatt aaccctatag atattgtgga agaaatagct
     1381 ataggatatg gaattgacaa tcttaaatct actctacctg catttcaaat ctcttcagga
     1441 gtaagaaacg atcattttag gaaaattgat gaaataaagg agacactgat aggacatgga
     1501 tttttggaga tggttaattt ttctatgtta gaccggacca tttctactat atttaatttt
     1561 gatgataaag aattacttga aaaaattaag gtaagtgaaa gcaaaagtaa ggaacatgaa
     1621 tttctaagga ctaatatatt atcctcttta gtcaataatc tttcctataa tgttcatgaa
     1681 gaatatcctc aaaaaatatt tgaaatagga aaaacattta gttttaaaaa taaaatagta
     1741 gaacaatgga atttaggagt cctccttgca tctaaaaatt ctaattatac agcagccaaa
     1801 tcaatattag aatctctttt taaaataaat tttggaaaag agtttacaac aagaagaact
     1861 gacaataatt tattcataaa aggtagatct gcaaatatat tatacgaaag taaaataata
     1921 ggttttattg gagaaatctc gcctgatttg atcactaaat tcaatttacg cattccgtta
     1981 tcagcctttg aattaaattt atcaactttt tttcaaagtt gtatggtaaa ataattcaaa
     2041 atcaatcttt tgatctccca tgtctcatgc gctagtcatt aagataattt tttataaata
     2101 aactttgggt tgtcttttta tattaacgca taattgtttt ataatggtat agatatatat
     2161 catttaccga atatttagct gaattattcg gtaaccccgt tatagagagt gcactaggag
     2221 aaatacacct ttgtgtatgg ctatatctat gaatagttgt gagtcagagg cgggggacaa
     2281 aactaaatag tcacaattta gttaactgca aataatgttc aaatccaata aatggattga
     2341 tttcaattaa tgaatatgtt attattgagt caaatcaaca atgcttttct tgataatata
     2401 tttagcttca tttgaaatat tatatttttc aagtatatct cccggattct ttattaatag
     2461 atttctaaat tcaggttcgc ttgcagccct gagcagaata tcacttatta tttctgcttc
     2521 tttctgtcgc gaaatcaata tagacaaaaa aacgtgaact catatataat cttaatgtta
     2581 aattagtaaa cttgataaga actttaatca atattttttt tagtattgat attgcgaagt
     2641 caaaaaaaat ggacattgaa tggaacatcc agagtaatac cagtggaaga aactcttctc
     2701 cgtgtttcag aaataagtaa agaaattggc cttactagaa ttgctgatat aacttatatg
     2761 gataaattat acataccgaa ttattcatgc gttttacctg gtaccgaaga ttatatatgg
     2821 gtatatagtg gaaaggggcc aactaaacaa catgcgaagg taagtgcaat tatggaaagc
     2881 attgaacgct attcttcctt acctacctat tatcatagta aatttattag tggaactttt
     2941 aatgaactaa aacaatctta taatattcta caccctgacg aagttataga accacttact
     3001 tttgattttc aaaatgatat gttaatggat tatgtcaaag ggtatgatat tattaataaa
     3061 gaagatatct tagttcctgc cgcattagca ctatttagat ttactccaca ctcgccttct
     3121 gtcaatcctt ttgcatttca tcatacaaat ggactagctt ctggcaacgt ttttgaagaa
     3181 gcagtttgtc atgcattatg cgaagttatc gaaagggacg ccatcagctt agctcaatta
     3241 cgtgcaagtg caataccatt ccatattctg tatagtatat ataataaatt taaattacaa
     3301 ggatatcatc ttgatgcatt taccaaacac gactttaagg atgataattc actatatcat
     3361 gatgttctaa tttctgaaga agttattagt ccaatatcta aattaattaa aaaatttaaa
     3421 aaatttaaca tagatttaat tattaaggat attactacag atattaaaat tcctaccttt
     3481 aatgttgcct gtgtcgaatg gatatctcat gattatggtt atttggctga aggacacggt
     3541 actcatccag ataaaagaat agcattatta agagctataa cggaagtgtc ccaaacaaga
     3601 gcagcaaata tccaaggtgc ccgagatgat ttaaggaaaa taaaatacaa tgataagaat
     3661 acagatgaca aaaatgcatg gcaatttatg tcttcacata agacaattaa gttttctgat
     3721 ataataactt atcaaaatga tgatatttta gacgatatca atctcattat taaatttttt
     3781 aaagaaatag gattgaataa ggctataata gtaaatttaa caaacccaaa aatcaaaatt
     3841 ccagtggtga gggctatcgt tccaggtctt gagacattta aaatatctaa gtctgttatg
     3901 ggctttcgag caaggagatc ctataaatga atttaccaat tatttttttg gggccatctt
     3961 tacagataaa caaagcaaaa aaaattatta acgcagactt tagaccacca gccaaaaagg
     4021 gcgactttat aaatttaaat ttattatccg agaaaagaga aatagttcta atagatggag
     4081 tgttcctcca ggactatcca ccaactccaa ttgaagtctt tcaagttatc aataataaaa
     4141 atttcgaggt gtatggcgca tccagtattg gagcattacg cgcagtagaa ttagaaaaat
     4201 ttggaatgaa aggcattgga aaaatctttg agctgtttaa aaaaaacata gtcaattcgg
     4261 atgatgaaat tgcagtaaca tttgattcaa attataacct tctttcggaa gcaatgattg
     4321 atattagata taatattttt ttaggctgga gaaatggaat aattgataaa gaaacaaagc
     4381 agattatgat aaagttagct aagaggatct actttccttt tcggacgtat gagaacataa
     4441 tcaagaaatc gcgtcatctt tttccctcaa aagaaaaata catatcaaga tttaatgaat
     4501 atatagttgt taataaagtg agtctaaaag agttagatgc catccgcgcc cttaaataca
     4561 tttcgtcatt aacaaaaaat aagaataatt aatttaaatt tattttccaa gctatactct
     4621 tctaataaac tattttatat catacctcgt aatctatatt atgactatga atcgctccaa
     4681 attaaatgaa cattataaaa caaactgtaa gaaaatactt gaactctcac ctaaaattcg
     4741 ttatgtaggt ataacaaata aatttggtag aacaatatca ggccaactta gaactggtat
     4801 cattccattt ttaaaaacag aagaaatgag aaatgagttg tttgttcatt ctatgattta
     4861 taatattaga aaaagctatg aaaaatctat aggagaaacc gaattcatat ttatttctaa
     4921 tgaaaatgtg gatatcgtta gtttttatat ggatgattta gtttattata tgactataga
     4981 taagaacata gataaaaatg aattatatac tgtggtcgaa aaaactagag gtatcgcaaa
     5041 atcataattg ttataaattt attgctgcaa attaagttaa attttttaat tttccccgca
     5101 tttccttatc tttataaatt gaaggatgac ttggatcagc taatatgacc tcgtaccatg
     5161 aatgtttacc atctttatag agaaaatacg aacccattac ttccatattt ggatattttt
     5221 cattaactct tctttcagct actaacttca tatttgtcgc ttgtttaata tgaacaactc
     5281 cactgtgttt cggccttcta ccggcgaccg gtcgctgctt tctcatgcct cctcttccca
     5341 cgcgtgctct aataattaca ataccctgct tggctttata tcctagtctt cgtgctctat
     5401 ctatcctgct aggtctattt atcctatgaa ttgatagctc ttttctccat cccacagctc
     5461 gagattttaa ttcctcgcta tttgtcttcc acattgtaat ccaagttttc gtcatctgtc
     5521 tgtacatttt aaaatataaa caaattcttt tcaaaggcta tataagaatt tctgttgtta
     5581 acccgtatca tttttttacc gtataaaaac catataaaga tgagaaggag agttaaatcg
     5641 aaattataaa atcaatttct attgtgcttc ctaatggaag actttcaacc ccaatggcga
     5701 tccgtgaatg ttttcctttt tcaccaaata acttttccaa aagatttgat gcagaattaa
     5761 taaccgttgg atgatcagta aaagattcaa gacagtttat gtatgcagat aactttacta
     5821 tttgtttgat tttatcaagg ctgcccaaat gctcttttaa taatgctagt gcgttaagtg
     5881 tacaaagtat tgccgcgtct tgaccttttt caagcgttgt gtcttttcct atttttcctt
     5941 tatactttac tgtagcaggt tttgaaactg tatctaaagg aatttgccct gaaatgaaga
     6001 ttagaccatt tacttccacc aaggggatat aagacccagc agattttgga gaggggggca
     6061 attcaaaacc taatgcatat agtttttcat caatgtttat caataataaa atatgaaaaa
     6121 attgtattat taaatgtatt aaacatttaa aataaattga taaaaataat aattttatac
     6181 atgataaagc gagttttatt gtcactagcc gtaggagtta taattactta tttagtttat
     6241 ctaggtctca tggattcctt aactagatct tcagttaatt atctctccat ctctaacgac
     6301 actgctattt ctcttatttt taaaaattat aacttatcgt ctcaacatct cactaacata
     6361 aattctaaat atgtctatat aaaaggaaat ggtgaaatat ttgaatctaa aaaagatact
     6421 aacttcatag gcaattatat aggaaaaact tctccaacta ttactactgg aaatcatttt
     6481 ggatgggaag tacaattccc tgcattaaat aaaacttatt acgttgatca tctaagagga
     6541 gacattatct caaccaagta aaaatccttt tttttatatt tattggtttg attatacata
     6601 taataagtaa taattatatt ggtatctaaa aaaatttatc catgcattta accggaaatg
     6661 ttttagtctg tgaccctata tcaaaaattg gaataacaat tttaaaaaaa gctggtctta
     6721 atgtcgtgga gaaaccctcg ataactaact ccgaattaga atcagaagtt gaaaattttg
     6781 aggtaattgt agtcagaagc agaaccaaaa tcactaatga aattatttca aaagcaaaaa
     6841 atgctaaaat aatagctaga gttggagtag gactggacaa tatagatgtt gaatctgctc
     6901 gacaaaataa tatcgaggta ataaactcac cagaatctgc aattaatgcc gttgcggaat
     6961 tagttgttgg attgatgctt tcattatcaa gaaatttaac gttggcagat agtgaaatga
     7021 agaaagggaa ttggatgaaa aaggaactga atggcattga attgagaggg aaatatttag
     7081 gaattgtagg tgtaggaaac attggaagaa acttagcaag aattgctaga tcattacgta
     7141 tgaatattat aggttatgat acctttccaa ttaagcagga cttcataaat gaagtaggaa
     7201 tgataacaac tgattttaag acattagtag cgagttctga ttttatttca tgtcatgttc
     7261 cgttagcgcc tgatactaaa catatgttca atgaagctac tttttcaaat atgaaatcta
     7321 cagctttttt tatcaattca tcaagaggag aaatagtaga tgaaaacgca ttatattcgg
     7381 ctttaataac aaaaaaaatt gctggagcag cgttggatgt ttttgaagtc gaaccaccta
     7441 ctaatataaa attaatgcaa ttaccaaata ttatttgtac tcctcatata ggggctcaaa
     7501 ctatagaatc acaagattta gcttcgaatg taatagcaga aaagattatt cataggttat
     7561 tagagaaaga gacgtaaatt tatatgaaag aatggaccgg gtgggatttg aacccacgac
     7621 cacctacgtt tcctctgcgt tgctggcatg caaggcaggt attctaccag gctaaactac
     7681 cggcccgtta atcaaaacct gattttctga taatttaagt gtttaattat tgttagataa
     7741 tctagaaatt ttaaccaatt aggcttacgc tattatagac agttaagttt attataatat
     7801 caaatattgg ttttgtaatt aatgctctgt aaattatagc actaagaaga tatcaattaa
     7861 taaaatatga ttatgttaat gaaaaaatca acaaaatagt taatatcctc tagtaataac
     7921 aaaatggtta gaatagagta taaatgacca cttgcagaga atattttacc gaacaaaaat
     7981 gtaaattttt atattacatt tttttactaa aacatttaaa atattgttct agagtaatta
     8041 taatagttgg ttttattgat attacttttt tatatgttct tatattatag atacttacaa
     8101 atatctctga cccaaagaaa aagaaattaa tacagttaaa gaatttgtta gttattctta
     8161 aattaaatac gattacagta gtgtaaattt ttataaagtc aattactata ttatttctgt
     8221 gtttgttaga ggaattttgg cttactgagg aagacaagag gtctatcaag aacaattctt
     8281 aattgtgaat gaattgaaat agtttttaca ccggctaccg tttcaaagag caataatagt
     8341 catagctaaa aaatgatatt tagagaatta attgtaagta atagaaccaa ttaattaatc
     8401 cattttctgc tagaagtgct acaggaaata ctattactta actttcatcg cttcattgat
     8461 atttccataa tattccatga cattcgagaa atgatttgga tagatgatta aaatcgaaac
     8521 gcttttaggt ttttcaagtt ttaataagaa tatgtctgtt ttcttaaaag atagagtttg
     8581 gataatgctt tctgaaattt ctaaacgagg tgtatatccc cttcatggat ataaattggg
     8641 tatttttagg ttgccagtaa atttaacaga ggaatcaaca atcaacaata tctataatga
     8701 gttaaagaaa acaggtttta agttagatag atatgctgat cgcatatatg taacccacat
     8761 ttggaaagaa caagaaagag ataatttgtt agctacatta ttatccatct cacttgatat
     8821 aacggtagaa attgtaacag gagaagttat tgatttaata tatcaaatta aacctattga
     8881 aaaattcggt gatagttacc ggataaataa ttatagacaa aaggcagact tcaatgcaaa
     8941 aatggtaatt gatacaatca taagaaatac ttcaattggc gataagttaa tttcttatta
     9001 ccaaaaaact gagaaattat cactcgaaaa agctattgat aagcttacac aattaactcc
     9061 tttggccctt aatccaataa ctagaaatgt taatccccca ccccctccag caggcgcatc
     9121 aatttcctct caagaaatat cttcatcttc cgtcaccaca accagatctg cgccaaatat
     9181 tccaaatatc ccttctgcaa ccactatgac ttctggaaat tctgatacaa ctatttctct
     9241 cacaggaagt ggaacagatc attccaaagt tgatggtcct attgatacct cttttaaatc
     9301 aaaaaggcaa aaagctggaa aatttaaagg tattagtgtt tggggaccag ttgaccctcc
     9361 tggacagttg ggaatatggg gc
//
LOCUS       DASVEV010000175        11709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1626233, whole genome shotgun sequence.
ACCESSION   DASVEV010000175 DASVEV010000000
VERSION     DASVEV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626233"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF222_08035"
     CDS             <1..487
                     /locus_tag="VF222_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEX7142411.1"
                     /translation="NPFQSEYFTVVNGWGKRDEVLKDENPAIIMATSGMLEGGPSVEY
                     FKDIATKENNKMIFVSYQINGTLGRRVLDGNTNEVSMMDKNGKVKVIPLKCKTLKIDG
                     FSGHSDFNQILNFVSRIKPKRVLVNHGERTKSENTASTIYNKLGIRSSVPDNREILRL
                     R"
     gene            578..724
                     /locus_tag="VF222_08040"
     CDS             578..724
                     /locus_tag="VF222_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142412.1"
                     /translation="MAIKYKCLECNIVLSGEGASRHYLSNPTHKLEKLPPPLKPTKIN
                     REEN"
     gene            complement(1181..2944)
                     /locus_tag="VF222_08045"
     CDS             complement(1181..2944)
                     /locus_tag="VF222_08045"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7142413.1"
                     /translation="MDFLTIAQIFEDMQKTRSRLLLIDHLVILFKTTPVNTIKKTIYL
                     LQGKLSPTYEGVELGLGEKLTIKAIAESSGISVKEVDDKYGLIGDLGETAKEIAKIKY
                     QQTLLSEKITIERIYSTFEKIAKSTGSHSQIIKLRLVSSLLNDATPLESKYIIKFLIG
                     TLRLGIAEYSIIDALAITFTGNRTNRKKIEETYNVYTDLGTIAEILSKENLQGLNELQ
                     ITPLKPIRPMLAERILDPREGLKKGSGKIAFEYKLDGERVQIHKDESEVQLFSRSLEK
                     ITHHYPDIVEAIKKFSIHKCILEAEIVPLDLKSKKIQPFQELMHRKRKYNINDIVLKY
                     PIKVHLFDILYLEGKDLTQYEYTKRRAILESIIKKNKNDIIQLVQRIVSSNLNQIENF
                     FSKAEDDGCEGLMLKQLNSKYRAGAREFLWMKLKKEYGNGNTLGDSFDLTVIGALLGK
                     GKRTGYYGALLLAVYDKNSDTFQSICKVGTGFSDRDLELIYNELKNYVVEEKYYNVNS
                     NMKMDRWFTPRLVLEIRASEITLSPSHTAAYGLVRKDLGLALRFPKFSGRIRCDKKPE
                     DSTNTSELIELFKKQVKRNKF"
     gene            3043..3366
                     /locus_tag="VF222_08050"
     CDS             3043..3366
                     /locus_tag="VF222_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142414.1"
                     /translation="MGGVKKKTLSSSKTTGQQPSDGTNKPKKDETKPQIKSQRHKSSV
                     LIEDINDVNILKGMKAITVQSISKLLGVKISIANNYIRNLESKGTVKLIGGYSGHKVY
                     SINTS"
     gene            complement(3375..3767)
                     /locus_tag="VF222_08055"
     CDS             complement(3375..3767)
                     /locus_tag="VF222_08055"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX7142415.1"
                     /translation="MPSENQFSSVSRIAIKVIIENKYVITGQLIRHLAPLTISELLRV
                     LPLHGAVHYNLDTFCYIQTQLNIGQEKPRKKFAKGDITLMTSNGSICFFLKDAAVSYA
                     MNLIGKLTSNIEVLGNLRPIDTLSLTRI"
     gene            3851..4366
                     /locus_tag="VF222_08060"
     CDS             3851..4366
                     /locus_tag="VF222_08060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF002207.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HEX7142416.1"
                     /translation="MVEELNTKYIDKPLEDTKLENEKDATQKKSSKNKKKEPRSKSET
                     KTPKSRIKNKKVLEAEEQISQEPESLRKEEFVEYQESEYSLREITSPAQEILIGPKRL
                     TRFEKARITGARSLQISFGAPLMIKGDVNARSSISLAIEELEKKALPISIRRTLPNGI
                     YQDIPIEWLKT"
     gene            4416..5099
                     /locus_tag="VF222_08065"
     CDS             4416..5099
                     /locus_tag="VF222_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142417.1"
                     /translation="MIEEGNSKSSLATPPLNLLFNTSLLGKQNVWEINISRLLELLLK
                     IINSTGKKDLRICGIAAWSSTLIYRLKVESIFQLEKLSLEKKNYESSIEKDIPHLKSI
                     DFPFRLSSTYPVSLEDLLKVLENIIYSLENPSMKSRNVIQLEPIENFDFDQYLVKFEK
                     ILEEKEEFIIHSLKLTETILFSKFIEKLGRLEVARIFIALLHLAMKGKIDLVQLEESN
                     DIEISRLLL"
     gene            5140..5655
                     /gene="scpB"
                     /locus_tag="VF222_08070"
     CDS             5140..5655
                     /gene="scpB"
                     /locus_tag="VF222_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7142418.1"
                     /translation="MKESLPEKEIVARIEAALYSSGRPMSIEELVKAAGLNSKEKIRK
                     LLLDLTKKINNVFYALELKELEDGKYVFQLKSDYAPTIRKFAKQPLLQSSSLKTLSYV
                     VYEQPVTSKRLVQIRGSQAYSHVKYLEQLGFIEHESVGRLKVYKTTKKFQDYFGISDL
                     NIIKNSMTLKP"
     gene            5691..6083
                     /locus_tag="VF222_08075"
     CDS             5691..6083
                     /locus_tag="VF222_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HEX7142419.1"
                     /translation="MRKSIEGLGGKKFTGGRLTPSRMRRKSEIDRYPNEPIVGKDSIV
                     SRRVRGGNHKLAFKHIEFLNVNDKENKKTIKTKILRVVKNPANKDYERRGVLSKGAFV
                     ETELGIVRIVSRPGQNGSINAILTKETA"
     gene            6180..6497
                     /locus_tag="VF222_08080"
     CDS             6180..6497
                     /locus_tag="VF222_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HEX7142420.1"
                     /translation="MKDYDHFIIWLDYFNKNLSRKKGRRIKRDMAVFDPSFSELFDAA
                     LEEGLGVTLEHSNDHARFPRRPYVRSGYIMISKALSKQKSEVLSLLSSKILSKRTKIK
                     RVK"
     gene            6567..6890
                     /locus_tag="VF222_08085"
     CDS             6567..6890
                     /locus_tag="VF222_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HEX7142421.1"
                     /translation="MKTRNLQYKFLTDVGEIIHKAKSGRLILKLFEETKIIAGDTILD
                     SEGKKIGNVNELIGSVNSPYASVILFNEAFEPRPGEKVFRFISKNPSKRFSTGFKKFR
                     GQKRR"
     gene            6899..7999
                     /locus_tag="VF222_08090"
     CDS             6899..7999
                     /locus_tag="VF222_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX7142422.1"
                     /translation="MVFLEYCSNCPECNSGLIHDFSKGEFLCQKCGYVVLDEVNDYGP
                     ESISKDFEEKTRIARASGTMSFSQHDYGLRTEIGSGLKDYGGKAINSQMAEHLTSLRK
                     WHSRIRVSSSKERRLSNVLSKITETCSNLSLPKILIETAAMIYRNYEKNKEAKGKSVS
                     CISAATIYLACKRCSIVRSLDEIVEATGISSNDRSSLKLASKYYRMLSMELGMYNGNF
                     SEQDGKNNQDQNSDKSKISYFLSNYQVIKSHDNTYISKPKNMLNGISHSLSIDHYIAK
                     LINLAKLDTRIERLAIDLAHKTAKNLLVDGKSPNGIAAAYIYLSSVLLGMNILQIDVS
                     GLAGVTEVTIRNRCKEILCSSKIIITIRPTLK"
     gene            8042..8347
                     /locus_tag="VF222_08095"
     CDS             8042..8347
                     /locus_tag="VF222_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142423.1"
                     /translation="MSDFFEIERLVEDLAKIYSTACATSWYKVNNISNPSIDEFRNKV
                     VDFMKHFEYTLSTFPQNRESEKFKKYARSLLYKEIERVQNGENKEVDKRYKYFVDYI"
     gene            8816..10285
                     /locus_tag="VF222_08100"
     CDS             8816..10285
                     /locus_tag="VF222_08100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7142424.1"
                     /translation="MSQNIPDYDIIIAGGGLAGMIVATSAAYYSKQSLKILVVDRNSF
                     SDLGKKTMNGWICGDAVGKNSVDYMTSRIGISWCWPEIEHSVKGVIAYSPDHKTGISF
                     DGDGYVLNRRLLPQKQLQDARKFGVEIMENIALRSLLKDGRFIIGVEGEDLKTKKIIK
                     KTAKLTIDCTGVTSVLRTNLPIQSYIQKKIDRDDLESTGRYIYNFKVNGEDKTYFDPD
                     YCIIHLDQNLAPGGYAWVFPKGTDKVNIGLGVQQLAFNLYNKSNGTNKNLKHLIDDYV
                     KINPVITEPELPNTEKDQDNTWGTWQVSVRRQNDCLVGNGYMLVGDSAWMPKPLDAGG
                     IGPAIIAATIAGKNAVESIQASDVSESGLWKYNTDFMSEYGYKTAGLEIFRRMLQSLS
                     NEQIDYGMKNFLSKFDIEKIAQGEHPEFSTVDKLGMLIKGVLNKNLAGDLKYTSQMNK
                     KLVDHYHDYPQNPEGFTNWKYKLDLYLKEAYSYFKEIKM"
     gene            complement(10493..11185)
                     /locus_tag="VF222_08105"
     CDS             complement(10493..11185)
                     /locus_tag="VF222_08105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7142425.1"
                     /translation="MIGLGYTYWKQVISILRDIIPVYDKVNRVISLGKDDLYRSEGIS
                     GNVKPGDLILDAGSGFGNMSLTTFKIITDEVSIVMYDPIFEMLQSSRQRLVVELLSSG
                     IFEHIPFKDNTFDMVMCGYSLRDSINLEVAISEIHRILKKNGRFLIVDLGKPNNKIVR
                     LGVTIYLKYIIKFFGYVIAKETGLKFQTLYGTFLKWPQNNTLYSLLKNQFSSVEFRSK
                     LMGGSIIVSSTK"
     gene            11299..>11709
                     /locus_tag="VF222_08110"
     CDS             11299..>11709
                     /locus_tag="VF222_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142426.1"
                     /translation="MAREFICKDCGSLLTHSNELTLESMKEIHSQLCIIKRQQLIAAQ
                     SIQEISPKSSAMGQSTIQVSQGSSNGTSQIEPSFNKPITGETTISLTGSGTDHSKVDG
                     PIDTSFKSKRQKAGKFKGISVWGPVDPPGQLGIWG"
BASE COUNT         4174 a         1705 c         1912 g         3918 t
ORIGIN      
        1 taatccattc caatcagaat attttactgt agttaatgga tggggaaaaa gagatgaagt
       61 attaaaggat gagaatccag ctattatcat ggctacatca ggtatgttag agggcggtcc
      121 ttctgttgaa tattttaaag acattgcaac aaaagaaaat aataaaatga tatttgtttc
      181 ataccaaatc aatggcacct tagggagacg agtgcttgat ggtaatacaa atgaggttag
      241 tatgatggat aaaaatggta aagtcaaagt tattcctcta aagtgtaaaa ctctaaagat
      301 tgatggattt agcggacaca gtgattttaa ccaaatattg aatttcgttt ccagaataaa
      361 acctaagcga gtcctagtta atcatggaga acgtacaaag tctgaaaata ctgctagtac
      421 aatctataat aaactaggaa taagatcctc agtgcctgat aatcgagaaa ttctaagatt
      481 aagataatta agtttatttt tttcaagttt tttcactaat ttagcataaa caatagactt
      541 tttagggaag tgtgtttaca tacttctggg ctggattatg gctataaaat acaaatgttt
      601 agaatgtaat attgttcttt ctggtgaagg agcttctcgt cattatcttt cgaatcctac
      661 acataaattg gaaaaattgc cacctcctct taaaccaaca aagattaata gagaagagaa
      721 ttgaaatttc acttaaagtt tccactattg aaattaattg cgatataatg gtacaaatac
      781 gtgacttaat aagcagtatt tggattaata ttgtctaaaa gattaaatta aaagagagtc
      841 ttaatcatat ttctacattt tttcatttcg taaaatcgat tgaaataatt tcactcgaac
      901 ctgaaatctg taatagtact attttgtaaa ttattactag caagataatc gatttgaata
      961 gtttttccaa tttattttgt ggagacttta cctagtctct ttttactatt atctttttgg
     1021 aataagatta ttattggttg tgtgttatat tacagtcgaa attgtaatat tagtatataa
     1081 aatcattatc aaaaagaaag aaaaagtatt tttctattta ggaattatat atgtcaaaaa
     1141 cttatgactg taagagtaag aaataaaaag tatcaagaaa ttaaaatttg ttccgtttta
     1201 cttgtttttt aaatagttct atcaattcac tagtatttgt agaatcttct ggctttttat
     1261 cacatcgtat tcttccagaa aatttgggaa atcttaatgc caatccaaga tctttacgta
     1321 ctaaaccgta agcagctgta tgggatggac tgagggtaat ttcagaggcc ctgatttcta
     1381 gaaccaacct aggagtaaac catctatcca ttttcatatt actattgaca ttataatact
     1441 tctcttcaac aacataattt tttagttcat tataaataag ttctaaatcg cgatcagaaa
     1501 aaccagttcc tactttacaa atactctgga aggtatcaga atttttgtca tatacagcta
     1561 ataacagagc accgtaatag cctgtacgct ttccttttcc taataatgct ccgattactg
     1621 taaggtcaaa actatctcct aatgtattac cattaccata ttcttttttt aacttcatcc
     1681 ataagaattc cctggcgcct gctcgatatt tactatttaa ttgctttaac attaatcctt
     1741 cacaaccatc atcttcggcc ttactaaaaa aattctctat ttggtttagg tttgatgaaa
     1801 caattcgctg tactaactgt ataatgtcat tcttattttt cttaatgata gattctaaaa
     1861 tcgctcttct ttttgtatac tcatattgag tcagatcttt tccttctaga tagagtatgt
     1921 caaataagtg taccttaatt ggatacttta gtacgatgtc atttatgtta tattttcttt
     1981 ttctatgcat taattcttga aatggttgaa tctttttaga ttttaagtct aatggtacta
     2041 tttcagcttc taaaatgcat ttgtgaatgg agaatttctt tattgcctcc acaatatcag
     2101 gatagtggtg agttattttc tcaaggcttc ttgaaaataa ttgtacctct gattcatctt
     2161 tgtggatttg gactctttca ccatcgagtt tatattcaaa agctatttta ccactccctt
     2221 tttttaatcc ttccctagga tcaagtattc tttcggctaa catgggtcga ataggtttta
     2281 gtggtgtgat ttgtaattca tttaaacctt ggagattttc cttagataaa atttctgcaa
     2341 ttgttcctag atcagtatag acattataag tttcttcaat ctttttccgg ttggttctat
     2401 ttccggtaaa ggttattgcc aaagcatcga ttatagagta ttccgctatt cctaatctca
     2461 aagtgcctat gagaaattta ataatgtatt tgctttctaa tggtgttgca tcatttaata
     2521 aactactaac tagacgaagt tttataattt gagaatgtga acctgttgat tttgctatct
     2581 tttcaaaagt cgagtatatt ctttctatgg taattttctc gctaaggaga gtctgttgat
     2641 atttaatttt tgcaatttct tttgcagttt cgcctaaatc tccgattaga ccatatttat
     2701 catcaacttc tttaacacta attcctgagg attctgctat tgctttaatg gttaattttt
     2761 ctcctaatcc taattcaacg ccctcatagg ttggagaaag ttttccttgt aggaggtaaa
     2821 ttgttttttt aatggtatta actggggtag ttttaaaaag aattactaaa tgatctatca
     2881 ataataacct gcttcgagtc ttttgcatat cttcaaaaat ttgtgctata gttaaaaaat
     2941 ccaatactga tctatcttac tctgattatt tataaaatat gatagagatt tttataacaa
     3001 atataaaaat atataactta aagtaagcat attagaatta taatgggtgg agtaaagaaa
     3061 aaaacattaa gttcatctaa aactacagga caacaaccta gtgatggaac taacaaaccg
     3121 aaaaaggatg agactaagcc tcaaataaaa agtcaaagac ataaatcttc tgttttgata
     3181 gaagatatca acgatgttaa tatactgaag ggaatgaaag caattactgt acaatctatt
     3241 tcaaaactct taggagtaaa aatatcaatc gcaaataatt atattagaaa tttggaatca
     3301 aaaggaacag ttaaactaat tggaggctat agtggacata aagtttactc tattaatact
     3361 agttaacaaa ctcttcagat tctagtcaaa cttagtgtat ctataggcct taaattacca
     3421 agtacttcaa tatttgatgt tagtttccca atgaggttca ttgcatacga aacagctgca
     3481 tctttcagga aaaaacaaat cgagccattt gatgtcatta aagtaatatc accctttgca
     3541 aatttttttc ttggtttttc ttgacctata tttaattgtg tttggatgta acagaatgta
     3601 tctagattat aatgtacggc tccatgaaga ggtaaaactc ttagcaattc agatatagta
     3661 agaggagcta aatgacgtat aagttgccct gttataacat acttattttc tattattact
     3721 ttaatcgcta ttctagatac tgatgaaaat tggttttcac ttggcataat acccacttaa
     3781 taaaggttgg ccagcaatta ccttaaattc gtaacgttta attatattta aattagcttt
     3841 tgcagtattc ttggttgagg aattaaacac taaatatatt gataaacccc tagaggatac
     3901 taaattagaa aatgaaaagg atgctaccca aaagaaatcc tccaaaaata agaaaaaaga
     3961 gcctcgttca aaatctgaaa caaaaacacc aaaaagtcga atcaaaaaca aaaaagtatt
     4021 agaagcagaa gaacaaatat ctcaagaacc agaatcttta cgtaaagaag aatttgtaga
     4081 atatcaggaa tctgagtata gcttaaggga aatcacaagt ccagcccaag aaattttaat
     4141 tggtccaaaa aggttaacac gatttgaaaa agcaaggata actggagctc gttctttaca
     4201 gatatcattt ggcgctcctc ttatgatcaa gggggatgtg aatgcaagaa gctctatttc
     4261 tctcgctatc gaagaattag aaaaaaaagc attgccgata tctattagaa gaacattacc
     4321 taatggaatc taccaggata taccaataga atggttgaaa acttaatacg tacaatacgt
     4381 ttttatagtt tcgagctttt attttaggct tacgaatgat tgaagaaggt aatagtaaat
     4441 catccttagc aacccctcca ttaaatttac ttttcaacac ttctctatta ggaaaacaaa
     4501 atgtttggga aataaatatt tcaagattat tagaattact tttgaaaatt attaactcca
     4561 ctggaaaaaa agatcttcga atttgtggga tagctgcatg gtcttcgact ctaatatatc
     4621 gattaaaggt tgaaagcatt tttcagttag aaaaactttc tttggagaaa aaaaattatg
     4681 agagttccat tgaaaaggat attcctcatc taaaatcaat agattttcct tttagattat
     4741 catcaaccta tcctgtatct ttggaagatt tacttaaagt actagaaaat attatttact
     4801 ctttagagaa tcccagcatg aaaagtagaa atgttataca attagaacca atagaaaatt
     4861 ttgattttga tcagtattta gttaaatttg aaaaaattct ggaagaaaaa gaagaattta
     4921 taatccattc tttaaagtta acagaaacta tcttatttag taagtttatt gagaaattag
     4981 ggcggctaga ggtagcaaga atatttatag ctttactcca tcttgccatg aaaggaaaga
     5041 ttgatttagt acaattagaa gaatccaatg atatagagat tagtaggtta ctactctgac
     5101 cgaatattaa taaatctcct ttaaaaaacc atattaattt tgaaagaaag tcttcctgaa
     5161 aaagaaattg tagcaagaat tgaggcagcg ctatattcct ccggtcggcc gatgtccatt
     5221 gaagaattag tcaaagcagc aggactaaat tcaaaagaaa aaataagaaa actacttcta
     5281 gatttaacca aaaaaattaa taatgtgttc tatgcattag aattaaaaga gctagaggat
     5341 ggaaagtatg tctttcaact taaatcagat tatgctccaa cgataagaaa gtttgctaaa
     5401 caaccactac tgcaatcatc atcattaaag actttgtcct acgtagtata tgaacaacca
     5461 gttacttcta agcgactagt gcaaataagg ggaagtcaag cctattctca tgtcaaatat
     5521 ttggaacaat taggatttat cgaacacgaa tcagttggga ggttaaaagt ctataaaaca
     5581 actaaaaaat tccaagacta ttttggaata tctgatttga atataataaa gaatagtatg
     5641 actttaaaac cttagaaaag tcttaattag acgttttatc aatttaaaga atgcgaaaat
     5701 ccattgaagg tttgggtggt aaaaaattta ctggaggccg gcttacgcca tcaagaatgc
     5761 gtcgaaaatc tgaaatcgat cgatatccca atgaaccaat tgtaggaaaa gatagtatcg
     5821 tttcaaggcg agttagaggg ggaaatcata aattagcatt caaacatatt gaatttctta
     5881 atgtaaatga taaggaaaat aaaaaaacta tcaaaacaaa aatccttaga gttgttaaga
     5941 accctgccaa caaagattat gagagacgtg gagttctgag taaaggagct tttgttgaaa
     6001 ctgaattggg aatcgtaaga atagtttcaa gacctggtca aaatggaagt ataaatgcta
     6061 tactgacaaa ggaaaccgct tgaagaacgc tacaaatgtg tgtcgtattt gctttgagtt
     6121 ttacgcacta ttgttttata actgaaaatt aaaataatga taatctctca ttaaacttat
     6181 tgaaagatta tgatcatttt attatttggc tagattactt taataaaaat ttatcaagga
     6241 aaaagggaag aagaattaag agagacatgg ccgtgttcga tccatcattt tctgaattat
     6301 ttgacgcagc actagaggaa ggattaggcg taacgttaga acattcaaat gatcatgccc
     6361 gttttccacg ccgtccatat gttcgatccg gatacattat gatttctaaa gcccttagta
     6421 aacaaaagtc cgaggtcctt tctttgttat cttccaagat attatcgaaa agaacgaaga
     6481 ttaaaagggt aaaataatca aaggtatttg taatttacat tttaatttct tttatcaatt
     6541 ttattaatat atgttgaccg aaggatatga aaacaaggaa tttgcaatat aagttcttga
     6601 cagatgttgg tgaaattatt cataaggcga agagtggtcg ccttatttta aaactttttg
     6661 aggaaacaaa aattattgct ggcgatacaa ttttagattc cgagggaaaa aaaattggaa
     6721 atgtaaatga acttattggt tcagtgaatt cgccgtatgc ttcagtaata ttgtttaatg
     6781 aggcttttga gcctagacca ggtgaaaaag tcttcaggtt tatctctaaa aatcccagta
     6841 aacgtttttc aacaggattt aaaaaatttc gtggtcagaa acggaggtag aaaatgtatt
     6901 ggtttttcta gaatactgca gcaattgtcc cgaatgtaat tctggtctaa ttcatgattt
     6961 tagcaaaggc gaatttcttt gccaaaaatg tggatacgtt gtattagatg aagtcaatga
     7021 ttacggtcct gaatccatat ctaaagattt tgaagaaaaa actcgtattg ctagagctag
     7081 cgggacaatg agtttttctc aacacgatta tggcttgaga acagaaattg gaagtggttt
     7141 aaaagattat ggaggaaaag cgatcaattc tcaaatggca gaacacttaa ctagtttaag
     7201 aaaatggcac tctagaatta gggtttcttc atccaaagag agacggttgt cgaatgttct
     7261 atcgaagata accgaaacct gttcaaatct tagtttacca aaaatactaa ttgaaaccgc
     7321 agctatgatt tatcgtaact atgaaaaaaa taaagaggca aaaggaaaat ctgtttcttg
     7381 tatatcagcg gctacaattt atttagcatg caaaaggtgt tcaattgttc gatctttaga
     7441 cgaaattgtt gaggcgaccg gtatatcttc taatgacagg tctagcctga aactagcgtc
     7501 aaaatactat aggatgctat ctatggaatt aggaatgtac aatggcaatt ttagtgaaca
     7561 agacggtaag aataatcagg atcaaaatag cgataaatca aaaatatctt atttcttatc
     7621 gaattatcag gtaatcaaat cacatgataa tacttatata tctaaaccaa aaaatatgtt
     7681 gaatggtatt tctcattctc tttcgataga tcactatatt gccaaattaa tcaatcttgc
     7741 taaattagac acaagaatag aaagactagc cattgattta gcacataaaa cagcaaaaaa
     7801 cttgcttgta gatggaaaat ctcctaatgg aattgctgca gcttacattt atttatcctc
     7861 agttctgttg ggaatgaata ttctccaaat tgatgtttca gggcttgcag gggtaacaga
     7921 agtaactatt agaaaccgat gtaaagaaat tctttgtagt tccaaaatta ttatcacaat
     7981 tagaccaact ttaaaataat atttcttaaa tttgaaatat cttttatact aaaccaatca
     8041 tatgtctgac ttttttgaaa tagaaagatt ggttgaagat ttagctaaaa tatactcaac
     8101 agcatgtgcg acttcatggt acaaagtaaa taatatttct aatccaagta ttgatgaatt
     8161 tagaaacaaa gttgttgatt ttatgaaaca tttcgaatat acactttcta cttttccaca
     8221 aaatagagaa agtgaaaaat ttaaaaaata tgcaagaagc ttattatata aagaaataga
     8281 aagagtgcaa aatggtgaaa ataaagaagt agataaacgt tataaatatt ttgtagatta
     8341 tatataagga gtctattctc caattcatgt taaatcaggg tgtttaaaag gtgttaaaat
     8401 tattaataca ttcggatttt ttctaacccg catagttttt tttagagttt ataccatcac
     8461 gccttaattt gttgaacctt atttggactt tatttagcta attactgttg aaaggattct
     8521 tgaaccacat tcgtgcttaa ccggatttct ttcgacataa gaattcaaac ttaaagttgt
     8581 ttaatcattt aaaacagttg tcaaaagtta atttccaaat ttatttgcta tcttttcgat
     8641 aaaggatagt tacactctat attcagagtg tttgaatcat atgtttcaag ataagtcttt
     8701 tccatcagaa aaaaatttta ttttttaaat tatttagttt ttaaattact ttatgtattg
     8761 ttcagtcaac attgacttaa ctataaatat tgtacttttg caaaactctt aatttatgtc
     8821 acaaaatata ccagattatg acattatcat agcgggaggt gggttagccg gtatgattgt
     8881 tgctacatcc gctgcttatt attcgaaaca atctttaaaa atccttgtag ttgatagaaa
     8941 ttccttttcg gaccttggca aaaagactat gaatggttgg atttgtggag atgccgtagg
     9001 aaagaacagt gttgattata tgacaagcag aataggcata tcttggtgtt ggcctgaaat
     9061 tgaacattca gttaaaggag taatagctta ttcccctgat cataaaactg ggatttcttt
     9121 tgatggtgat ggctatgtac tcaatagaag gctcttacct cagaaacagt tacaagacgc
     9181 tagaaaattc ggagttgaaa taatggaaaa tattgctctg agatcattgt taaaagatgg
     9241 taggtttata attggagtag agggtgaaga tcttaaaact aaaaaaataa taaaaaaaac
     9301 tgcaaaatta acgattgatt gtacaggtgt cacctctgta cttcgaacaa atttaccaat
     9361 ccaatcttat attcaaaaaa agatcgatag agatgattta gaatccactg gtcgttatat
     9421 ttataatttc aaggtaaatg gtgaagataa aacgtacttc gatccagact attgtataat
     9481 tcatttagat caaaatttag ctcctggggg ttatgcttgg gtttttccga aaggtacaga
     9541 taaggtgaat ataggattag gagtgcaaca attagcattt aatttgtata acaaaagtaa
     9601 tggaactaat aagaatttaa aacacctcat tgatgactat gtaaaaataa atccagtaat
     9661 cactgaacca gaacttccga atacagagaa ggatcaggat aatacctggg ggacatggca
     9721 agtatcagta aggcgacaga atgattgtct ggtaggcaat ggttatatgt tagttggaga
     9781 ctcagcatgg atgccaaaac ctttggatgc aggaggaata gggccagcca taattgcagc
     9841 aacgattgct ggaaaaaatg ctgtagaatc tattcaggca tctgatgtat cagagagtgg
     9901 cctttggaaa tataatacag attttatgtc agaatatggg tataaaacag ctggtttaga
     9961 aatttttcga aggatgttac aaagtttatc aaacgaacaa atagattatg gaatgaaaaa
    10021 ttttctttca aaatttgata ttgaaaaaat agctcaagga gaacatcctg aatttagtac
    10081 ggtcgataaa ctcggaatgt tgataaaagg tgttttaaat aaaaatttgg ctggagattt
    10141 aaaatatacg tcacaaatga ataaaaaatt agtagatcat tatcatgatt atccacaaaa
    10201 tccagaaggc tttactaatt ggaaatataa attagatcta tatctaaaag aagcctattc
    10261 gtatttcaaa gaaataaaaa tgtaacatga ttttgagctg aggtattaat gggagttttt
    10321 gaaccttctt tatttctatc catctttccg tatcttttta tttctttgtt tccaatattg
    10381 ggcattataa ttgtgacgag atccatatta aaaaataagt aattttactt ttaaatatgc
    10441 cgtcaattta tataaattac aaataaatta tctaaataaa atgatatgaa acttattttg
    10501 tagatgatac aataatagat cctcccatta atttacttct gaattctaca gatgagaatt
    10561 gattttttaa caatgaatat aatgtattat tttgtggcca ctttaaaaaa gttccataaa
    10621 gtgtttgaaa tttaagtcca gtttcttttg ctattacata cccaaaaaat tttataatat
    10681 attttaaata tatggttact cccaatcgta ctattttatt attgggtttt cctaaatcta
    10741 ctattaaaaa cctcccattt ttttttaaaa ttcggtgaat ttcagaaatt gctacttcta
    10801 aattaatgga gtctcttaaa gaataaccac acatcaccat atcaaatgta ttatctttaa
    10861 aaggaatatg ctcaaaaatt ccagacgata agagttctac tactaatcgc tgcctagagc
    10921 tttgtaacat ttcaaatatt gggtcgtaca taactatact cacttcatct gtaataattt
    10981 taaatgttgt caaagacata tttccaaaac cagaacctgc gtctaatata agatcgcctg
    11041 gctttacatt tccgctgata ccttcagagc gatataaatc atcttttcca agtgatatga
    11101 ctctattaac tttatcatat actggtataa tatcacgtaa aattgagata acttgtttcc
    11161 aatatgtata ccctaatcct atcacatgta ggtttagttt gtctacatat ataatataag
    11221 aatattgtat ttgctaaaac tattttggca ttatctgcaa ccaaatattg ataaatggca
    11281 tattctataa aatatattat ggcacgtgaa tttatttgca aagattgtgg ttctctttta
    11341 acccattcca atgaattaac tttggaaagt atgaaagaaa tacattctca gctttgtatt
    11401 attaaaaggc agcaactaat agctgctcaa tctattcaag aaatttctcc aaagtcttct
    11461 gctatgggac aatctactat tcaagtttcc cagggctcca gtaatggtac atcacaaata
    11521 gagccttcat ttaataagcc tataaccggt gaaacaacta tttctcttac aggaagtgga
    11581 acagatcatt ccaaagttga tggtcctatt gatacctctt ttaaatcaaa aaggcaaaaa
    11641 gctggaaaat ttaaaggtat tagtgtttgg ggaccagttg accctcctgg acagttggga
    11701 atatggggc
//
LOCUS       DASVEV010000176         8793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1042758, whole genome shotgun sequence.
ACCESSION   DASVEV010000176 DASVEV010000000
VERSION     DASVEV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042758"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            605..2731
                     /locus_tag="VF222_08115"
     CDS             605..2731
                     /locus_tag="VF222_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7142427.1"
                     /translation="MKYKDTKPNKLIKESSPYLLQHAYNPVEWFPWNSEALTKAKKEN
                     KPIFLSIGYSACHWCHVMAHESFEDENIARILNTKFVSIKVDREERPDLDDIYQKACQ
                     LVMGNGGWPLSVFLTPDQKPFYVGTYFPKQSRYGMPGFSTILETLSQAYHDKKEDIDK
                     ATNELMDSLIKSSSDLAKNPQTEIEKTILDESAINLLQIADSLNGGFGQAPKFPNTTN
                     LLFLLRYFDYSKNNNFLNFVEFTAKKMAWGGIHDHIGGGFSRYSTDQKWLIPHFEKML
                     YDNALLIQLFSELYQATSKNQYLTLIQTTIQYLLREMTLMNEKNECAFYSSQDADSEG
                     VEGKFYTWSQNEIKNNLRDNQKLELFCDYYGITQGGNFEGTNILNITKSKETLSKKYN
                     ISIDKVEDILNESLKGLFNVRKNRIAPGKDEKILTSWNSLMISALVSAYKVTNNEEYL
                     EYAKNTINFIEKKLAYDSNRLKHTYKDGISKINGYLDDYSYYISSLLDVFEVTSNPFY
                     FQRAIQYLDSMIKHFWDDKEKNFFFTPDDQEQLILRTKNFYDLAIPSGNSMAVYALLR
                     LHFLTQNPDYLDKAESIIKSCYKAALGNPFGFGQLLISIYLYFKKPLEIIILQNKNNN
                     HNKSELKSWIMKQYIPNSITLSFEHSSDELQQLMSSNIFTLLKGKKIDIADGDKFKEC
                     VYICRDFTCSLPMYTLSDLKNYLNSN"
     gene            complement(2781..3251)
                     /locus_tag="VF222_08120"
     CDS             complement(2781..3251)
                     /locus_tag="VF222_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7142428.1"
                     /translation="MVKNHLFSKILVPIDGSEPSFHAAQIASNVAHKFNSEIILLYVV
                     VSPSHYEYINLTGLVTPKQIDTIIENAKKESKNWFTRIENIVKDANPNIKVTTKVILT
                     GVAVYGEILQHAEKENIELIVIGTRGKSGFKKLLLGSTASGVVTYANCPVLVTK"
     gene            3570..4442
                     /locus_tag="VF222_08125"
     CDS             3570..4442
                     /locus_tag="VF222_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX7142429.1"
                     /translation="MNDSPRIPFDCGVTVEDQLYLAYEKKIDALFITNHNTLNGYKQM
                     VEYRDNHEKLKSIKIYPAEEITINTKGHVLAYGIYETIKPGMNIDETLDEIKKQNAVS
                     CAAHPFAVSNGIRENAIKCDMIETFNSNNIDHYSNIVAEKFSSDNKMISIAGSDSHVL
                     TTFGRCINSIESENKLDSLLQEMLKRKSKILKAELALKEELFEHAHYMLSSSKDQLLN
                     YILVHHPSKFSLVKWTLDSFLSKPNSKLWKILGSLGLYLSKRVSKKVNMKGHNPYVFQ
                     DRSWKTLISMSLYP"
     gene            4515..5039
                     /locus_tag="VF222_08130"
     CDS             4515..5039
                     /locus_tag="VF222_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142430.1"
                     /translation="MFYKKGLTLVPGILLVSFLFVHNYISVDGQAQQLNSSMTIMDTK
                     KNISDVQNSNWTGSVEISKVLRDSFNPLIKISLSEAIDKSEVNIGNNSSAVAAFIHPV
                     KGYLVYVIYLLNDQNAVTKVITDVGKGNILNVTKMTIEEMMFNFHHGGMTKSNSPAFG
                     KNNMMDTMMLNTPY"
     gene            complement(5119..5895)
                     /locus_tag="VF222_08135"
     CDS             complement(5119..5895)
                     /locus_tag="VF222_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX7142431.1"
                     /translation="MKILVGKYVLLYHTMNKKWLIKVEEEKKMHTHLGIIDVSSLIGL
                     EYGMSIKTTLDKKIYLIEPTIHDFIMKSERNTQIIYPKDLGYIAIRSGIQNGSKVLEI
                     GTGSAALTTFLASIVKPNGHIFTYDINPEFMKIAKKNLVKAQIDEYVTMTEQDIRQGI
                     DRNDFDAAIVDLGDPWTAIDVAYTALKGSGIFLAICPTMNQAEKTSVSLKNNQFTDIE
                     CVEILIRNIEAREGMTRPSMRMIGHTTYLIFARKIFSSEI"
     gene            complement(5953..7908)
                     /gene="argS"
                     /locus_tag="VF222_08140"
     CDS             complement(5953..7908)
                     /gene="argS"
                     /locus_tag="VF222_08140"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7142432.1"
                     /translation="MTLKEVIHQIREIINKSIVDLKYETIEYDVSEPPKTEFGDLTTN
                     IAFLLSKRMHKKPNEIARELVYNSINLQLKKMGKDSLIRNADAHISGHINFNINYVNF
                     NKFLLAMVKKNKLLLPNIGQNKKIVIEHTSVNPNKALHIGHLRNVVVGDSLYRLFKMT
                     NHKTLVLNYIDDSGVQVADLIVAFRFAGFNPDNMDKNFKFDQYCGEIYVKMNELYKTN
                     LDLIEKRKLVIKEIEQGNTELAHFTNDIVEKIVKQQLVSCWRIKSRYDLMVIESQILL
                     SKLWEKTLNLLKDRNIIRFSTEGKNINCWVIKDEDNEEKVVIRSDGTATYIAKDISFA
                     VWKLNLVDDPFTYTKFSLQWDNSILWKSMLKSETTPNSLHEKNLVLGENSPSKVITVI
                     DSRQARLQKIISSILSEIDPKIQNYIFLGYETVALSPYTVQAIGKELDEKNKKVIHMS
                     GRKGIFINADNILDELHKKASSEIRKRNSDFDDTTTEEIAEGIAVSAIRYNLLKQDLD
                     KMITFDMKEALNLDGDTSLYLQYSFARAMRILEKGRSTLEEFINERNDNIRFDLLNHK
                     IEVELIKEISKFNLIIEEALNSLNPKILAKYANKISTKFNLFYENLPVLGTDLFLKVN
                     RLMLVMVFVNVLANLFEILGIESFSRI"
     gene            complement(7905..8618)
                     /locus_tag="VF222_08145"
     CDS             complement(7905..8618)
                     /locus_tag="VF222_08145"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX7142433.1"
                     /translation="MTSIIKAFAIDIDGTLTENGGGIIHLPAMQDLRTLEKLGYRIIY
                     VTGRSSVEAYMLSVFGGTTKIAVGENGGVVTLSPTEHILLGNKEKCINGYRILTRKLE
                     NVKIKNVFDRMTEVVLERTFDIELGKQILNENNISVDLSDSSYSFHINNRGINKATGL
                     AKALDILKIKPSETVTIGDSETDIPLFQFCAFSIALNHADETVKSKANYVVSGNSGKG
                     LVEALDYIALNFFGYNAIK"
BASE COUNT         3042 a         1353 c         1240 g         3158 t
ORIGIN      
        1 aatgtgtatg aatggttgga aacacctggt catccaggtt ctgagaaaaa accttttatg
       61 gctagagaaa aagattgtat attttgtcta gcatgtgaaa atgtgtgtcc accacaagcc
      121 atcaaaatct ttgtaaaata acccaacttc cttcaagttt attgactcca taaccataat
      181 tacaatatcc ttggattata ttctaataag taaatgattg caagaaagtg tcctagaact
      241 atcttcaatt aaattattga aaattcgaga ttatagatat gagaaaaaat tatggttata
      301 tttgttgtaa agatttagat actatgaaca gcatttgaat atcgtgtgct tcttttttga
      361 attaggttat attaaatcgg aagaacatct atcataaatg aataaattcc ttctttaaaa
      421 acaggaatgg gaaattaatg aaatttctta ttctttttgc gttctaagaa aaattgatat
      481 acccaatctt ttattacctc tctattacta tatttaacta cttataccta gataaattag
      541 attgtacctt ctaataaaca taataaatac aattaaaaga tcgtaatacc atagttaaaa
      601 atatatgaaa tataaagata ctaaacctaa taaattaatt aaggagtcaa gtccttatct
      661 tttacaacat gcctataatc ctgttgagtg gttcccttgg aactctgagg cattaactaa
      721 agctaaaaaa gaaaataaac caatctttct tagtattgga tacagtgcat gccattggtg
      781 tcacgtaatg gcacatgaat cctttgaaga cgaaaacatt gcgagaattc tgaatacaaa
      841 atttgttagt atcaaagtgg atagagaaga gaggcccgat cttgatgata tataccagaa
      901 agcatgtcaa cttgttatgg ggaatggtgg ctggcctttg tcagtatttc tgactcctga
      961 tcagaaacca ttctatgtag gaacttattt cccaaaacaa tccagatatg gaatgccagg
     1021 attttcaacc atattagaaa cattatctca agcttatcat gataaaaaag aagatattga
     1081 caaagcaact aatgagctta tggattccct tataaaatca tcatctgatc ttgcaaaaaa
     1141 tccccagaca gaaatagaaa aaacaatttt agacgaatca gcaataaacc ttctccaaat
     1201 tgctgattct ttaaatggag gttttggaca agcccccaaa tttcctaata caactaatct
     1261 tttattttta ctaagatatt ttgactattc caaaaataat aattttctta atttcgtaga
     1321 atttacagcc aaaaagatgg cttggggagg gattcatgat cacataggag gaggattttc
     1381 aagatattct actgaccaaa aatggcttat tcctcatttt gagaaaatgc tatatgataa
     1441 tgcattgtta atccagttat tctccgagtt atatcaagca acatcaaaaa atcagtattt
     1501 aacgttaatc caaacaacga ttcaatattt attgagagaa atgactctta tgaatgaaaa
     1561 aaatgaatgc gcattttatt catcacaaga tgcagattct gaaggagtag aaggaaaatt
     1621 ttatacctgg tcacaaaatg aaattaaaaa taatctaagg gataatcaga aattggaatt
     1681 gttctgcgat tattatggaa taactcaagg aggtaatttt gaaggaacaa atattttgaa
     1741 tattacgaaa tcaaaagaaa ctttatcaaa gaaatataat atctcaatag ataaagttga
     1801 agatatcctg aatgagtcat tgaaaggttt gtttaatgtc agaaaaaata ggattgctcc
     1861 aggaaaagat gaaaaaattt taacttcttg gaattccttg atgatttctg ctcttgtctc
     1921 agcatacaag gttactaata acgaagagta tttggagtat gcgaaaaata ctataaattt
     1981 cattgaaaaa aaattagcat acgatagtaa tagactaaaa catacctata aagatggaat
     2041 ttcaaaaatt aatggatatt tagacgatta ttcatattat atcagttctc ttttggacgt
     2101 ttttgaagta acttcgaatc cattttattt ccaaagagcc attcagtatc tagattctat
     2161 gatcaaacat ttttgggatg ataaggaaaa aaatttcttt ttcactcctg atgatcaaga
     2221 acaattaatt ttaagaacaa aaaatttcta tgatttagca atacctagcg ggaactccat
     2281 ggcagtttat gccttattac gtcttcattt tcttactcaa aacccagatt acttagataa
     2341 agcagaatca attatcaaat cttgttataa agcagcatta ggaaatcctt ttgggtttgg
     2401 tcaattatta atttcaattt atttatattt taaaaaacca ttagagataa ttatcctaca
     2461 aaataaaaat aataaccata acaaaagtga actaaaatcg tggattatga agcaatatat
     2521 tccaaatagt ataacattaa gttttgaaca ttcatctgat gaattgcagc aattgatgtc
     2581 gtccaatatc tttactcttt taaaaggaaa aaaaatagat attgcagatg gtgataaatt
     2641 taaggaatgt gtatacatct gcagggattt tacatgttca ttacctatgt acacactatc
     2701 cgatttaaaa aattatctta atagtaattg acaaattatt ggttagtgaa atatatataa
     2761 ctaaattata tgaatttttc ttatttcgtg actaatacgg gacagtttgc atatgtaact
     2821 acacctgatg ctgtgctacc cagtagtaat tttttaaatc ctgactttcc tctcgtaccg
     2881 ataactatta actcaatatt ttctttttcc gcatgttgta gaatttctcc ataaacagcg
     2941 acgcctgtta atatcacttt ggtcgtcact ttgatatttg gatttgcatc tttcactata
     3001 ttttctattc ttgtaaacca atttttagac tctttttttg cattctcaat gatcgtatca
     3061 atttgtttgg gtgtaacaag acctgttaga ttaatatatt catagtggga cggagaaaca
     3121 actacataaa gaaggattat ttcagagtta aatttatggg cgacattact agcaatttgc
     3181 gcagcatgaa aagagggttc tgatccatct atagggacaa gtatttttga aaatagatga
     3241 ttcttaacca taaaaacaat gctaaattga gataaataaa tcttgtattt tgaataagat
     3301 aaaaaagaaa ataaaaatgt ttttttatga tatggaacta actgcacatt ggtgaattaa
     3361 aaatatgagg acgatgcgct cgtgatgcat ttttgaagta tgtaatgaac aagactttga
     3421 ttgaaattga aaaatgtgat attagctact ttttaatata tatttataga tagtcatata
     3481 taagagaaat tacccattaa attgggttta tagatacgac tcaattatta aatatggaat
     3541 tacactgtca taacgttttt tctaattata tgaatgattc acctaggatt ccttttgatt
     3601 gcggtgttac cgttgaagat cagttatatt tagcatatga aaaaaaaatc gatgccttgt
     3661 tcattactaa tcataacaca ttgaatggat ataaacaaat ggtagaatat agagataatc
     3721 atgaaaaatt aaaaagtatt aaaatatatc ctgctgaaga aataacaatc aatacaaaag
     3781 ggcatgtttt ggcttatgga atatatgaaa ctataaaacc cggtatgaat attgatgaaa
     3841 ctttagatga aataaaaaaa caaaatgctg tttcttgtgc agcacatcct tttgcagttt
     3901 ctaatggaat tagagaaaat gcaattaaat gtgatatgat tgaaactttc aatagtaata
     3961 atattgatca ttattcaaac atagtagcag agaaattttc ttcagacaat aaaatgatat
     4021 caatagcagg aagtgactct catgttttaa ctacttttgg aagatgcata aattctattg
     4081 aatcagaaaa taagttagat tctttattac aagaaatgtt aaaaagaaaa tctaaaattt
     4141 taaaagctga gcttgctcta aaggaggaac tttttgaaca tgcacattat atgttgtcat
     4201 cttcaaagga tcaactttta aattatatat tagttcatca tccatcaaaa ttttctttgg
     4261 taaaatggac tttggactca tttctatcta aacctaatag taaactctgg aagatccttg
     4321 gttcgttagg attatatctg tcaaaaaggg tttcaaaaaa agtcaatatg aaaggacata
     4381 atccctatgt ctttcaggat agatcttgga aaactttaat ctcaatgagt ctttatccat
     4441 agttatagtt tttttgcata attaactatc aaatattttt ataattgttt aatcattcat
     4501 ttactatgag aaaaatgttc tataagaaag gattgacatt ggttcctggt attttgttgg
     4561 tttcctttct atttgtacac aattacatat cagttgatgg acaagcacaa caattgaata
     4621 gtagtatgac gataatggat acaaaaaaaa atatctctga tgtccaaaat tctaactgga
     4681 cgggatctgt agaaatatca aaagttctga gagactcttt taatccctta attaaaatta
     4741 gtttgtctga ggctattgat aaatctgaag taaatattgg gaataactcc tctgctgtcg
     4801 ctgcattcat ccatccggtc aaaggatatc tagtatatgt gatttattta ctcaatgatc
     4861 agaatgcagt aacaaaagta attacagatg taggaaaagg aaatattcta aatgttacaa
     4921 aaatgacaat tgaggaaatg atgttcaact ttcatcatgg aggaatgaca aaatctaata
     4981 gcccggcttt tggaaaaaat aacatgatgg acacaatgat gctcaacact ccttattaaa
     5041 ttctaaacat gcattctaaa cctaaatcac aaatttaaca ttactttatt ttttttataa
     5101 tactaattta actccctatc atatttctga cgaaaatatt tttctagcaa aaattagata
     5161 ggttgtatgt ccaatcattc tcatagatgg tcgagtcata ccttctctag cttcaatatt
     5221 acgaattaga atttctacac attctatatc tgtgaattga ttatttttca atgaaaccga
     5281 agttttctct gcttgattca ttgtaggaca tatggcaagg aaaataccgc ttccttttaa
     5341 tgcagtatat gcaacgtcaa ttgcagtcca cggatcaccc aggtctacaa ttgccgcatc
     5401 aaagtcgttc cgatcaatac cttgtcgtat atcttgttct gtcattgtta catattcatc
     5461 tatctgtgct ttaacaagat ttttttttgc tattttcatg aattccggat taatatcata
     5521 ggtaaaaata tgtccatttg gttttacaat acttgctaga aatgttgtta aagctgcact
     5581 tcctgttcct atttctaata cttttgatcc attttgaatt ccacttctta tagctatgta
     5641 tcccagatct tttggataaa taatttgagt atttctttct gacttcataa tgaaatcatg
     5701 aatggtgggt tctatcaagt aaattttttt atctagagtc gtttttattg acataccata
     5761 ttccaaccca attagtgaag aaacatcaat aattccaagg tgggtgtgca tctttttttc
     5821 ctcttctact tttataagcc atttcttatt catggtatga taaagcaata catatttacc
     5881 gactaggatt ttcatatttt ttattttaaa taatctacaa ttaatattta ttatagaaaa
     5941 aagatttgat cttcatattc ttgaaaatga ttcgattcct aatatttcaa aaaggttagc
     6001 taaaacgttt acaaatacca ttactaacat tagtctattt acctttaaaa atagatctgt
     6061 ccctaaaaca ggtaggttct cataaaacaa attgaattta gtagatattt tatttgcata
     6121 tttcgctaaa atctttggat ttaatgaatt taacgcttct tctataatta aattaaactt
     6181 tgatatctct ttaataagtt ctacttcaat cttgtggttt aagagatcaa atcttatatt
     6241 atcatttctt tcattgataa attcttctaa agtgcttctc cctttttcta gaattctcat
     6301 agctcgtgca aatgagtatt gtaaatacaa tgaagtatct ccatcaagat ttaatgcttc
     6361 tttcatatca aaagtgatca ttttatctaa atcctgttta agcaagttgt atcgaattgc
     6421 tgaaacggca attccttcgg cgatttcttc agtggtagta tcatcgaaat cagagtttcg
     6481 tttcctgatt tcagaagaag ccttcttatg caactcatcc agaatattat cggcattaat
     6541 aaatattcct tttctcccag acatatgaat aactttcttg tttttctcat ctaattcctt
     6601 tccaatagcc tggacagtat aaggactaag agcaacagtt tcatatccga gaaatatgta
     6661 gttttgaatt ttgggatcta tttcacttaa aatactagaa attattttct gtaatcgtgc
     6721 ttgtctagaa tctattactg ttattacttt actagggctg ttttctccca aaactaagtt
     6781 tttttcatgt aaggaatttg gggtagtttc tgactttaac atacttttcc ataaaataga
     6841 attatcccat tgtagagaaa atttggtata ggtaaatgga tcatccacta aatttaattt
     6901 ccaaactgca aatgaaatat cttttgcaat atatgtagcg gttccgtcac ttcttattac
     6961 caccttctct tcgttatctt catccttaat tacccaacaa ttaatattct taccttccgt
     7021 tgaaaaacga ataatgttcc tatcttttaa caaattaaga gttttttccc acagctttga
     7081 taacaatatt tgtgattcta tcaccatcaa atcgtatctc gatttgatcc tccagcaaga
     7141 cacaagttgt tgttttacga ttttttcaac tatgtcattt gtaaagtgag ctaattctgt
     7201 gttcccttgt tctatctctt taataactaa ttttcttttt tcaattagat ctaaattggt
     7261 tttatacaat tcattcattt taacgtatat ttcaccacaa tattggtcaa atttaaaatt
     7321 tttatccata ttgtcaggat taaaacccgc aaatctaaaa gctacaataa gatctgcaac
     7381 ttggacgcct gagtcatcta tatagtttaa aacaagagtt ttgtgattcg tcattttaaa
     7441 taatctatat aatgaatccc ccactactac attcctaaga tgtccaatat gcaatgcttt
     7501 attaggattt acactggtat gttcaataac tattttttta ttttgtccta tgtttggtaa
     7561 taataattta ttttttttca ccattgctaa aagaaattta ttgaaattaa cataattgat
     7621 attaaagtta atatgacctg atatgtgagc atctgcattt cgaattaaag aatcttttcc
     7681 catttttttt aattgtaaat ttatagaatt ataaacaagt tctctagcaa tttcattagg
     7741 ttttttatgc attcttttgc taagtaaaaa agcaatattt gttgttaaat caccaaactc
     7801 tgttttggga ggttcagaaa catcatattc gatcgtttca tattttaaat ctacaataga
     7861 tttattaata atttctctaa tttggtggat tacttcttta agtgtcattt gatagcatta
     7921 taaccaaaaa aatttaaagc aatataatcc aatgcttcaa ctaagccttt tcctgaattt
     7981 ccagatacta cataattagc ttttgatttt acagtttcgt cggcgtgatt cagtgcaata
     8041 ctaaaagcac aaaactgaaa caaagggatg tcagtttcac tatctccaat tgttactgtt
     8101 tcagaaggtt ttattttcag aatatctaat gcttttgcaa gcccagtggc cttattgatt
     8161 cctctattgt taatatgaaa agaataactg ctatcgctta gatctacact tatattattt
     8221 tcattaagga tttgctttcc taattctata tcaaaagttc tctctaacac aacctccgtc
     8281 atgcgatcaa aaacattttt tattttaaca ttttctaatt ttcttgttaa aattctatat
     8341 ccattaatac atttttcttt gtttccaaga agaatatgct ctgtagggct aagagtgact
     8401 actccaccat tttctccaac ggctatttta gtagttccgc caaatactga taacatataa
     8461 gcttcgactg aagaccggcc ggtaacataa atgattcgat aacctaattt ttctaacgtt
     8521 ctaagatctt gcatagctgg aaggtgtata attcctccac cattctcagt caaagttcca
     8581 tcaatatcaa ttgcaaaagc tttaattatt gaagtcatat ggaataatat tacgttagga
     8641 atttaacctt ctctttttaa aaaatataat agtgaatttc attctccttt tacttcttct
     8701 cttgttctaa actttggtgt tattcctaga gaactataat tcccagttga acatgtaaaa
     8761 catagttctt cttcaggtat ccctattgct tta
//
LOCUS       DASVEV010000177         6313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_462854, whole genome shotgun sequence.
ACCESSION   DASVEV010000177 DASVEV010000000
VERSION     DASVEV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462854"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VF222_08150"
     CDS             <1..1602
                     /locus_tag="VF222_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7142434.1"
                     /translation="RSKIGVKVGILVFIQLFFIISSFIILYTCQSQMFSFENSINIAG
                     NNRFLTSNLMYNIYDHLLEDSSDVSKIKTAMNDLESNIMTVKNGGKISGIDLKPLPSE
                     FSKDWDEIYQKWILLKTLLTDNILEQNQKINPVVIVTSEATTGSTLTPSKLELDTILK
                     TKIKSGISSLVDSTNLLVTQLGEHKKNMVQGILFYQWFFSLLTIGVTSAFVFYLVRKI
                     LKPIIALTHATSEVIRGNITVVVKTNSDDNDAYADDDELSVLTKSFNSMVTSLKNSIK
                     KQNELTKQLENANKVLENANMKLKERDQLKDEFINIAAHELKTPIQPILGLSELLLSR
                     QPNLQNDEEILNVIIRNSKRLMKLAEEILDVGRIESGSLFLKKEKFDLNELILTDILR
                     DYNQMIIENKKNIKLFYKSYDKNAIMVEADRDRLYQVICNLLSNALNFTNEGSITVIV
                     ERKGNEILVSLKDTGTGIHPQIYPKLFTAFVTKSPKRGTGLGLFISKSIIEIHGGRIW
                     AFNNSDVSGGTDKGSTFTFSLPYHE"
     gene            complement(1842..2219)
                     /locus_tag="VF222_08155"
     CDS             complement(1842..2219)
                     /locus_tag="VF222_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142435.1"
                     /translation="MFKKSIAIIDDDPDLLNLFSEALQMSGYTVSSFTDPLLAYQHIQ
                     ENPDKYSLVIVDEKIFNLNGLFSSTKLLEINPKLNVILLSNFKDFKYNYKFNILKKRV
                     SIFKLISAVNESISKSLSHDDKL"
     gene            2462..2776
                     /locus_tag="VF222_08160"
     CDS             2462..2776
                     /locus_tag="VF222_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142436.1"
                     /translation="MGSNIHSKIVITSSIIILASAFLIVIAGISSTTTFVNVNAQSEG
                     ANQTMQQQEDPQQVANQTMEKAIQSADETGEDVGKVGSKVTEGAKDLVGNIGDKLQDL
                     GK"
     gene            3127..3360
                     /locus_tag="VF222_08165"
     CDS             3127..3360
                     /locus_tag="VF222_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142437.1"
                     /translation="MSIENEDVSKTTTRPATEEEEYEEEFEIEIRTQKGSSIKVETTS
                     YNKLDGLLEKAFTTYQRLAKEESGGVDEKLQYQ"
     gene            4101..4364
                     /locus_tag="VF222_08170"
     CDS             4101..4364
                     /locus_tag="VF222_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142438.1"
                     /translation="MYIGRTSVRLGKFRCQNYINEFNRCRNRATKLAIIQLQQQQQDK
                     TPSLHQRIWICDDCFKVKFLHDNKFKDQLLQVTNLAKNKNDID"
     gene            4740..5042
                     /locus_tag="VF222_08175"
     CDS             4740..5042
                     /locus_tag="VF222_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142439.1"
                     /translation="MIFKNNDNKNSSVEFPYGKYPTNLLKLFEKSYENREGKERKLLY
                     CIAWCKDLTPATKPQILMDICVESYKTKGKFLHEMDKDEAKKTLNELLESEFRVHR"
     gene            5426..5743
                     /locus_tag="VF222_08180"
     CDS             5426..5743
                     /locus_tag="VF222_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142440.1"
                     /translation="MPVKLTTTLHKIDKIENVQNRLLLKQYHKFMDSTGASERHQNNN
                     LKVIYYYVNYLGKDIFLSSNTSCDSILLVLETKKNTKDEDPDGKWISTWNHYFHRIKH
                     FFR"
BASE COUNT         2473 a          879 c          925 g         2036 t
ORIGIN      
        1 cgttctaaaa taggcgtaaa ggtaggaata ctcgttttta ttcaactttt ttttatcatt
       61 agtagtttta ttatactcta tacttgccaa tcacagatgt tttctttcga aaactcgata
      121 aatatagcag gaaacaatcg atttctcacc tctaatctta tgtataatat ttatgatcat
      181 cttttggaag atagtagtga tgtatccaag attaagactg ctatgaatga tctagagtca
      241 aatataatga cagtaaaaaa tgggggaaaa atatcaggga ttgatctaaa accattgcct
      301 tcggaattct ctaaggattg ggatgagata taccagaaat ggattttatt aaaaacacta
      361 ttaacagata atatattaga acaaaatcaa aaaattaacc ctgtagtaat agtaacatca
      421 gaagccacca caggatcaac actaactcct tcaaaactag aattggacac aattctaaaa
      481 acaaaaataa aaagtggaat atcatcttta gtagattcaa caaatctatt ggttacacaa
      541 ttaggagaac ataaaaaaaa tatggttcag ggtatattat tttatcaatg gtttttttcc
      601 ctcttaacta ttggagtcac ttctgctttt gtattttatc tggtaagaaa aatactaaaa
      661 ccaataattg ctctcactca tgcaacatct gaagtaataa gaggaaatat cacggtagta
      721 gttaaaacca acagtgatga taatgatgct tatgctgatg atgatgaact ttcagtgcta
      781 acaaaatcct tcaattctat ggtcacgtct ttaaaaaact ctattaaaaa acaaaatgaa
      841 ttaaccaagc aacttgaaaa tgcaaacaag gtacttgaaa atgccaatat gaaattaaaa
      901 gaaagagatc agttaaagga tgaattcata aatattgcag cacatgaact caaaactcct
      961 atacaaccaa tacttggact ctctgaatta ctcctgagcc gccagccaaa cttacaaaat
     1021 gatgaggaaa ttctcaatgt tatcattagg aattcaaaac gactaatgaa actcgcagaa
     1081 gagatattag atgtcggaag aatagaaagt ggatctttat ttttaaagaa agaaaaattc
     1141 gatctcaatg aactgatatt aacagatatc ttgagagact ataatcaaat gataatagaa
     1201 aataaaaaaa atattaaatt attttataaa tcatatgata agaatgctat aatggtagaa
     1261 gccgatagag acaggcttta tcaagtcatt tgtaatcttt tatccaatgc attaaacttt
     1321 acaaatgagg gcagtattac tgttattgtt gaaagaaaag ggaacgaaat tcttgttagt
     1381 ttaaaggata ctggaactgg catacatccg caaatatatc ctaaactttt tacagcattc
     1441 gttaccaagt ctccaaaaag aggaacaggt ttaggattat ttatttcaaa gagtatcatt
     1501 gaaatacatg gtgggaggat atgggccttc aataatagtg atgtgagtgg tggcacagat
     1561 aaaggatcaa catttacatt cagcctacca tatcatgaat aaacagaatt ttaatttttt
     1621 atatcaaatt taattattta tcaattaact aggttatcta attcttatat aattcagaag
     1681 agagaatcta gatatatgga ttacaaaaaa ataatcactc aatcgatcaa tctgccaaca
     1741 agagaaagat attaggttag cctttgtctc aatagtcata gccttctatt gttatgatat
     1801 attcattaga aggttatggc ttttactaat attataaaga atcagagttt gtcatcatga
     1861 gaaagagatt ttgatatact ttcatttact gcactaatta atttaaatat agatacacgc
     1921 tttttgagaa tgttgaattt ataattatat ttgaaatcct tgaaattact taataatatt
     1981 acatttagtt ttggatttat ttctaagagt tttgtactgc taaataatcc atttaaatta
     2041 aatattttct catcgacaat tactaatgaa tatttatctg ggttttcttg gatatgttga
     2101 tatgctaaaa gaggatcggt aaaactagaa acagtatagc cactcatttg taatgcctca
     2161 ctaaataaat ttaataaatc tggatcatca tcaattatgg ctatggattt cttaaacatt
     2221 ctatagtata tagatctaac gtaaaaaatt ctatttaagt atagtttgta attttttctc
     2281 ttttttatta ttttactaat taattaatta aattttctat gatttttaaa tattataatt
     2341 agcaaatgac caatgcagac ataaatatat tcacacacaa agtcatagtc ttctaattag
     2401 aataatatta ataatagatg actatggctt ttaatagaag ttacatttat ctaaaaatga
     2461 tatgggatca aacatccatt caaaaatagt tataacatca tccatcatca ttcttgcatc
     2521 tgctttttta attgtaatag caggaatatc atcaacaaca acatttgtta atgttaatgc
     2581 acaaagtgaa ggagcaaatc aaaccatgca acaacaggaa gatcctcaac aagttgctaa
     2641 ccaaactatg gaaaaggcaa ttcaatctgc cgatgaaact ggagaagatg ttggtaaagt
     2701 tggctctaaa gtcactgagg gagcaaaaga cctggttggg aatattggag ataaattaca
     2761 agatttggga aaatagatat aacttaatat tttttaaatt aataatttcg atttgattgt
     2821 ttctaatact gcatctttta ttatgattca atcatgataa caacaatagc aagacaaagc
     2881 aaaaatatat tatccgataa aaaagcaaag gttatttctt cgtttatatc aatatttatt
     2941 tatgaatcct actgaatgag gattctaacc gaggattcag cgcgtggagt gagactaaaa
     3001 ccctaaattt ttattttttt attttacaga ttttgtttaa tttactacta aatcctttac
     3061 tttgtagtaa cattagttta attatcttgt tatatagttt attctacact atattctgat
     3121 aaaaatatgt ctattgaaaa tgaagatgta tccaaaacaa caacaagacc tgcaacagag
     3181 gaagaagaat atgaagaaga atttgaaata gaaattagga cacagaaagg ttcgtctata
     3241 aaggtagaga caacttctta caataaacta gatggtttat tagaaaaagc atttactaca
     3301 tatcaaagat tagcaaagga agaatctgga ggagtagatg agaaactaca atatcaataa
     3361 aataaatcat aagtttaata ttttaaagaa agtaattaat taatcaatct atatactcta
     3421 gtggtataaa aatagaaata atgaagagag caagaaggtt cattgaggct catgtaagat
     3481 acatgtcctc tagtctggtt tttcactcca cagaactcaa gacaaaatca tcttaaccaa
     3541 tctaagaaaa tatagatagt taaaatagat aaacaaagaa ataaaattta caattgatct
     3601 attttttaat taatctaata ctctagcgat aacctagatt ggtagttatt atatgttctt
     3661 gcaaaaaata aataaatgat tattgattta ctaggaatac ccttttacct tggctaaata
     3721 aatcttcttc agcctttgtt tatacactaa agcacgatta tagaatatag ttagataatt
     3781 agtatgagat tccataactt tatcataatt ttctgcttct ttacactcta attcatgtga
     3841 actaatattg atatcaccat cattccaata gtcgaaaaga acgactaaaa ttttatttca
     3901 cataagattg ttaaagacta caacccttaa aatatactag tcataaatct tgtatatctc
     3961 tagtgttttt tattgagtaa ttagaactat ttgctagtat aggagttgaa tattaaatat
     4021 tataaaacaa ttttcagcat aatattgaat aataagatta agaaatgcag aaatctgaca
     4081 aataagaaca taataatagg gtgtatatag ggagaacgtc agttaggtta ggaaaattca
     4141 gatgtcaaaa ctatataaat gaatttaata ggtgtcggaa tagggctacc aaactcgcaa
     4201 tcatacaact tcaacaacaa caacaagata aaactccgag tcttcatcaa cgaatctgga
     4261 tctgtgatga ctgttttaag gtaaaatttt tacacgataa taaatttaaa gatcagttac
     4321 tacaagttac aaatttagca aagaataaga atgatatcga ttgatgcttc attattgatt
     4381 attgattaaa ttctcaaact agatttacaa ctaatcataa ataaaagaat cactatgata
     4441 ttttaccaaa tatatcatca accaaggaat cacttgattt gtcatagtcg gaacaataat
     4501 cccagcccgg atttcactaa tagttcggct atgacaagta aattatatca aagtattaaa
     4561 tagaatacta tactcattca taccacacct ctctagcgat attactatta ttattagttg
     4621 ttgtgaggga taaccagaac aaagaagacc atcctctccc cctccctctc acttaggtag
     4681 gacacatagt aacaatagta ataataataa tatgatataa gatgtgatga ataagacata
     4741 tgatttttaa aaataatgat aacaaaaata gtagtgttga atttccatat ggtaaatatc
     4801 caaccaactt actcaaatta tttgaaaaat cctatgaaaa ccgagaagga aaagaaagaa
     4861 agttattata ttgtatcgcc tggtgtaagg atttgacacc tgcaaccaaa ccacaaatac
     4921 ttatggatat atgtgttgaa tcatacaaaa ctaaaggtaa attcctacat gaaatggata
     4981 aagatgaagc aaagaaaaca ttgaatgaat tactagaatc tgaatttagg gttcatagat
     5041 agtagataaa ggtagacaag ttatggaaca attttcagca ttattctgcc taaaactaat
     5101 agcgaatgaa aatattaaat tattaaatat tattcttaat aaataatatt acagtaacat
     5161 gctgtccagc catagtcatg ctctatatat gtcatcgttc catcatcatt attgaagtag
     5221 taggctatgg cttttaattt taaactttaa tgatatatta tataatcgat cattatgtgt
     5281 ctgggttaac tttgctaaca aagtatattc ctttaggccc attattctgt ggggattaaa
     5341 taggaatgct tacgtgaata aattaacaat aatgatgtgt tcagagaaga aaaacagaaa
     5401 atagatttag gaggaataat ctgatatgcc agtaaaacta acaacaacat tacacaaaat
     5461 agataaaata gaaaatgttc agaatcgctt gttactaaaa caatatcaca aatttatgga
     5521 ttctactggt gcatctgaaa ggcatcagaa caataatcta aaagtaatat attattatgt
     5581 taattattta ggaaaagaca tttttttatc tagcaataca agttgtgatt caatcctttt
     5641 agttttggaa acaaagaaaa acacaaagga tgaagaccct gatggaaaat ggatttctac
     5701 atggaatcat tactttcata gaattaagca tttctttaga tgagaaagaa aaaaaaatat
     5761 tattagaata gaaattcaag aaaatcctga tattttattt aaaaatttat tagttgactt
     5821 tattagaaat gacaattatc aaagtttaat agaattgtta gtataatttt atataaaata
     5881 ctaaatctag ccataaatta ggcacatcaa taatatcata gaaaagtgga cactgaaaat
     5941 aaactggaaa aagcgtaagt actttttcaa tcaaatgcaa gaaaatttag ataggctagc
     6001 ccatatttac aaatacatgc actaaggttc ttggtgaatg taccagaatc gcaatcttct
     6061 gttccagtat tgaaagttgt cacacgaaca ctaatttcat ctcctgcaga aaccaggata
     6121 cgctcttgta aaaatgttca gcaaaaatga acgtatccct ccattcccaa gaatattggt
     6181 cagtctgagc agacactttc aaattatcgc caagtgatgc tcctcgtgta tgtatataca
     6241 ggtctccagt ttattcattc ttttaatagt ctggtgaagt caatgaaggt tttagacatc
     6301 agaatagtta gtg
//
LOCUS       DASVEV010000178        11207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_465777, whole genome shotgun sequence.
ACCESSION   DASVEV010000178 DASVEV010000000
VERSION     DASVEV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465777"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VF222_08185"
     CDS             <1..607
                     /locus_tag="VF222_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7142441.1"
                     /translation="GNNNSQDPLDPEMFLNKLLSPSAANASALGNEQANITIVEFADY
                     QCPFCAQFNKETKDSIIENFVDTGKAKFMYKDLIVNDGSDKASTLAAAASYCAAEQGM
                     YWEYHDELFKNSKGENTGWVTKNSLMQFANNVRVPDLMKFSDCIDSDKYIEIVSENDS
                     FARNIGLQSTPSFIFYNGTTPVAIQGAQPYEAFEQIITAIS"
     gene            complement(693..1505)
                     /locus_tag="VF222_08190"
     CDS             complement(693..1505)
                     /locus_tag="VF222_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7142442.1"
                     /translation="MFEVDIFSYGFVQRALFSGLIIAITCSIIGIFLVLKKQSLFGDA
                     ISHMAFGGIAIGTFLNFYPIWTAMGFSIVTALAIAKMRQSTKLPSDSMVAVMLSLGLG
                     IGVTLVSLSNGFSVDIFSFLFGSILLVSPEDIYSILIVAVIVVSIIIVYYKKFIYITF
                     DEDQAKISGIEVEKLNYLFTILVAITIIVSIKLVGILLISSLIVIPNITALLFNRGFK
                     KTLLISLSLSTFSVFGGIILSYYYNLAPSGIIVLLSATGFIITFLLKTSILK"
     gene            complement(1530..2255)
                     /locus_tag="VF222_08195"
     CDS             complement(1530..2255)
                     /locus_tag="VF222_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX7142443.1"
                     /translation="MPLVSVQSVSYKYNSESVLENVSFNIEEGDFLGIIGPNGAGKTT
                     LFHCMLGLLNTYSGRITILNQDIKKNKKIFKKIGYIPQKKSIDQKFPLTVQELVSLSL
                     TRETPKNVVFEILKQVGLYNMKNKTIGQLSGGQQQRVLIAKALVNNPIILMLDEPTNE
                     LDQKSQHDFYFLLKELNEKNKITIIWTSHDMDAVNKYANKVSCINKRMFFHGDKSEFF
                     STEDHLRNYSESTMQIHMHEHDI"
     gene            2558..3931
                     /locus_tag="VF222_08200"
     CDS             2558..3931
                     /locus_tag="VF222_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7142444.1"
                     /translation="MIIQKESSTTHEMNKKRWIIRYFMLSSIGMILILKVYFMLFIVD
                     FTIGFYSFLTTLALFSIFLFSYLKFKDPLLKIKKKSIDIEPLITIIVPVKNEEENIEN
                     CIKSCINSSYKNKEIIVVNDGSTDKTPNILERLRKENPNALRVLHLPINLGKKHAIEK
                     ATEIANGEFYVFMDSDCNMADDAVKNLVKIFLSDKEIGAITGHGTVRNADTGNLLEKI
                     QDVWFDGQYRIIKGMESSFSSLTCCSGSLSAFRREAIINHIHEWANDRFLGMEFKFAT
                     DRRLTAFILAEKIKNRNEKLIKKNPTKEKGRKIKSQQWKLKYSPSVNVEIGVPQTLRS
                     LIKQQIRWRKSFIRSIFATGSIYWQRPLTVAIIYYLTIALRLFRPYIIIKSLIILPLN
                     GDILSGIFYLGGTMFAGLMYGIDVRLRKPGYPRWFYRPLMNVISTFILTWLIVYAAIT
                     IKNKTWR"
     gene            complement(4028..4963)
                     /locus_tag="VF222_08205"
     CDS             complement(4028..4963)
                     /locus_tag="VF222_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7142445.1"
                     /translation="MDTFNVLIPGAGGPAGINTIKSLRMSSFKGNIISTDSNSLSAGF
                     FLSDFYYVIPPYDNKFFLVNLLKIINEQNIKVLMPSSGFDIFGYSINHDLIVEYGAIP
                     IVSRRKVLEICRDKLLSYEFLSNKFPFAFTCEYDKEIDTFPLIAKPRFGKGSTNIIKI
                     ENKLDLDYVSRKFKNMIFQEYLPGIEYTVDVLSDLTEKPIMAIPRIRMDTKAGISVKG
                     KIKRDSLIENLCKKTAETLGIKGPCCIQLKESKDGELKIIEVNPRFGGGTIFTTLAGA
                     NFPAMLLEMISNNNLIIPQVSEITVLRYFEEIVIE"
     gene            5162..5779
                     /locus_tag="VF222_08210"
     CDS             5162..5779
                     /locus_tag="VF222_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7142446.1"
                     /translation="MNLKEDYHIHTNYNNHSASNLTVKNVVQEAERKGLEIIALTEHV
                     RESSEWIPSYLNEILHYKQKTKIKVIPGFEAKILFNGNIDCREEISRDYFIIASFHTK
                     YCDKVIWINALKKVIENKHVNVIGHLAPESSFEINPTEIEELAKLLKKHDKIVEVNAK
                     YQRPPETWIKIFINRGVKFHLGSDAHSLNEIGNYESIIKLIKLFE"
     gene            5887..6270
                     /locus_tag="VF222_08215"
     CDS             5887..6270
                     /locus_tag="VF222_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive regulator 1"
                     /protein_id="HEX7142447.1"
                     /translation="MPIVSISLNDDIINDIDKLQKNLGFTGRSEIVRAGLRNIIAEQK
                     ERDTLQGKLAALLLIIHQERDDDQVTNMRHDFEEIVTTHIHSKIDQDRCVELFLLKGD
                     AELIRNMTKKFQTNKKLEHVKLIAM"
     gene            complement(6293..6643)
                     /locus_tag="VF222_08220"
     CDS             complement(6293..6643)
                     /locus_tag="VF222_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142448.1"
                     /translation="MPNTSNVVNSEIMSLLKNQDSSESSVTINQVKNQNMKYFAKIVD
                     KNSNNEDTISSNSLTEFNNELLKFCLESGIDYYSCSSIIQDSVQSAQFTSTYMGPATV
                     NTDLIGYSLELKIK"
     gene            6654..7034
                     /locus_tag="VF222_08225"
     CDS             6654..7034
                     /locus_tag="VF222_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142449.1"
                     /translation="MGGEELGEEELGGSNELRGEELDEEELGGSNELRGEELDTEEFD
                     TEEFAVGVESGGEETDDEELGGSNELRGEELDTEEFDEGGEPIPCINDSIVLSIIFPL
                     PTLFKRLFISVSMFVELEPLSDCA"
     gene            complement(7319..9619)
                     /locus_tag="VF222_08230"
     CDS             complement(7319..9619)
                     /locus_tag="VF222_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX7142450.1"
                     /translation="MTTSYKIYENKMIRLILISFLISFTIFSSNLFQMNSVEAQIGKF
                     GTFTGHNFKYIKDSELFQLNQFSIAVWFKTNNTDPHVSFVVNKGGFGSEASGENMNYG
                     FWITYLGHLQAGFEAKDSSNYFVTSKKPVNDNKWHYATVTYDGSLLKLFIDGVFDHSL
                     NTKHAKPDITGKNRIAIAANSLFKDKLYVGDVDEVRIWDRPLTTAEIYDAYTQNKFNL
                     DNQLVFVSFGDNTSCKPEKAVNVTSIGHVGNNTELNAIDGNQSTRWSKYGLNSWTQLD
                     LGKEKEICDIDISWYKGDHRNVKFTISTSADGEIFSNLFSETSSGLTNGAEKFDIPDV
                     KTRFLKITVNDNSMNDWSSISEIDINNPIIPGSPSPCFPLNIPVNHISSIDTSLNNTK
                     GNLVDNNLSTKWSNLGLGSWIQFDLGQEKQICDIGISWYKGNIRNIDFVVSVSSDGYD
                     FRQVFSSTNSNTTDDIEVYDIPDAVGKYLRITVTGNSVNDWASINEVNLFANPIQKES
                     LTSASLINIVTQVINDNGATKQTKDFIVSVNGTQATPHSFAGNPDGQSVSIERGSYEV
                     KINNFTGYKTTLSAGCKGIIGLGDNRECVVTLDDIDTANTSNELTNTIDNFTSNNLTS
                     NNLTSNNLTSTIAITNKYINNSNNNDTPNINPLVRDLDLNVLLEVINVHGGTKKPLDF
                     IITVKGENVVPSSFAGSNNFQKVSIKPGNYDVKISNTFGYEINRISGCTGIIETTDKT
                     CIIELIDTPKLLDMSQINQTSGNLTQ"
     gene            complement(9788..10408)
                     /locus_tag="VF222_08235"
     CDS             complement(9788..10408)
                     /locus_tag="VF222_08235"
                     /inference="COORDINATES: protein motif:HMM:NF034126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HEX7142451.1"
                     /translation="MIKKKANYDIIFPGEHVAFIEEFESGKNTFVVDGSIKSLSLGKK
                     EYDFKKRQVAIERIKQAHFPKIGDVIVGYIEMLFGSMISIRILYLNNKKFSSGLSAIA
                     STRIASESGGWNSRRDRGERRPKFMYRVGDIVRGRIFSLLNSSAHATISDKDFGIVYC
                     ICYICGGDTVKSYNTVKCIECGMMEEKKLASDFGKEVLLSLGKKRE"
     gene            complement(10687..10977)
                     /locus_tag="VF222_08240"
     CDS             complement(10687..10977)
                     /locus_tag="VF222_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HEX7142452.1"
                     /translation="MKSKKENRNKENTDRLESTIATLESFKENKDLPRVIRNQIKDIY
                     DILKEEKNGSISVRAANAVSMLDSLTQNRHMESHIRTTLWQVVSKLESIREQ"
     gene            11132..>11207
                     /locus_tag="VF222_08245"
     CDS             11132..>11207
                     /locus_tag="VF222_08245"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HEX7142453.1"
                     /translation="MQCAVYDTYVTKKDGKIMHFDVIVE"
BASE COUNT         3990 a         1625 c         1648 g         3944 t
ORIGIN      
        1 aggtaataat aatagtcaag atcctctaga tcctgagatg tttttaaata aattgttaag
       61 tccatctgcc gctaatgcct ctgcactagg aaatgaacaa gctaatataa caatagttga
      121 gtttgctgat tatcagtgtc ccttctgtgc ccagtttaat aaagaaacta aagattctat
      181 aatagagaat tttgtagaca ctggaaaggc caagtttatg tataaggatc tcattgtaaa
      241 tgatggcagt gataaagctt caactcttgc tgcagctgcg tcctattgtg ccgcagaaca
      301 aggaatgtat tgggagtatc atgatgaatt attcaaaaat tcaaaaggtg aaaatacagg
      361 ttgggtaaca aaaaatagtt taatgcagtt tgcaaataat gttagagttc ctgatcttat
      421 gaaattttcc gactgtattg attctgataa atatattgaa atcgtgagtg aaaatgattc
      481 atttgctagg aatattggat tacagagtac accatcattt atcttttata acggtacaac
      541 acctgtagca attcagggag cacaaccata tgaagcattt gagcaaataa ttactgcaat
      601 aagctgatat atgctccctt tttttggttt tttatgacaa tgtggtcaat aaacaaccta
      661 ataatatacc aaattgaaac tatcccttta ttctatttca aaatcgaagt ttttagcaga
      721 aaggttataa taaaaccagt tgcagacaac aatactatta taccactcgg tgctaaatta
      781 tagtaataag atagaataat tcccccaaat actgagaaag tagacaaaga taatgagatt
      841 aaaagtgtct ttttgaagcc tcgattaaat aatagagcag ttatatttgg aataactatt
      901 aaagatgaaa ttaataatat tccaaccaat ttgatagaaa caattattgt tatagctacc
      961 aaaatggtaa ataaatagtt aagtttttct acttcaatac cactaatttt tgcttgatcc
     1021 tcatcaaaag taatatatat aaacttttta taataaacta taattataga cacaactatt
     1081 actgcgacaa ttagtataga ataaatatct tcaggactaa ctaatagaat acttccaaag
     1141 agaaaactaa atatgtctac agagaagccg ttagataaac taaccaatgt aacgcctatt
     1201 cccaaaccca aagaaagcat tactgctacc attgaatctg aaggaagttt tgtagattgt
     1261 ctcatttttg ctatagccaa ggcagtgact attgaaaatc ccattgcggt ccaaatggga
     1321 tagaaattta aaaatgtacc tattgcaatt cctccaaatg ccatatgaga aatagcatct
     1381 ccaaataagg attgtttttt tagaacaaga aatattccaa ttatagaaca ggtgatggca
     1441 ataattaaac ctgaaaataa tgcacgttga acgaacccat atgaaaaaat atcgacctca
     1501 aacatagaca aatcattttg gtatattttt catatatcgt gctcatgcat atgaatttgc
     1561 attgttgact cagaataatt cctgagatga tcttcagtag agaaaaactc acttttatca
     1621 ccatgaaaga acatcctttt attgatacat gatactttgt tagcatattt atttaccgca
     1681 tccatatcat gtgatgtcca tattattgta atcttatttt tttcattaag ttcctttaga
     1741 aggaaataga aatcatgctg acttttttga tccaattcat tagtgggctc atcgagcatg
     1801 aggatgattg gattattcac taatgctttt gctattaaaa cacgttgttg ttgacctcca
     1861 gaaagctgac cgattgtttt gtttttcata ttatataatc caacctgttt tagaatttca
     1921 aaaactacgt tcttaggagt ctctctagta agagataagg atacgagttc ttgaactgtc
     1981 agtggaaact tttgatcaat agattttttt tgtggaatat accctatttt tttgaatatt
     2041 tttttatttt tttttatatc ttgatttaga atagttattc ttccagaata tgtatttagt
     2101 aaacccaaca tacaatgaaa taaagtagtc ttacctgcgc cattgggtcc tataatcccg
     2161 agaaaatcac cttcttctat attaaaagag acattttcta aaaccgattc tgaattatat
     2221 ttgtatgaaa cagattgaac agaaactaat ggcattttgt cctctcctta aaatagaaaa
     2281 cgccttcatt aatataatca actttatgtt gcgtattttg cattgctggc aatagtattt
     2341 ctacttatat aataactatt attaattatt tatggtttat tattaaaaaa tataggactt
     2401 tggaactaga tttaataata aacgaattca gatttttcat ataatttttt atgataaata
     2461 tataaaaaat tatccactaa tagatttaat atttattact atattttata ataggctaga
     2521 atgctaacag ctaataaatt gaaagaatga tagagttatg atcatacaga aggaatcatc
     2581 tacaactcat gagatgaata aaaaaagatg gattatccga tattttatgt tatcatcaat
     2641 aggtatgata ttgatcctca aagtttactt tatgttattt atagttgact ttacaatagg
     2701 attttatagc ttccttacca ctcttgctct tttcagtatt tttttatttt cttacttaaa
     2761 atttaaagat cctctattaa aaattaaaaa gaaatccatt gacatagaac cactaattac
     2821 tataattgtt ccagttaaaa atgaggaaga gaatatagaa aattgtatta aatcttgtat
     2881 aaattcatct tataaaaata aggagataat tgttgttaat gatggtagta ctgataaaac
     2941 acctaatatt ctagaaagat taagaaaaga aaatccaaat gcactaagag tattacatct
     3001 tcctataaac ctaggaaaaa aacatgccat tgaaaaagct acagaaattg ctaatggaga
     3061 attttatgtt tttatggata gtgattgtaa tatggctgat gatgctgtaa aaaatcttgt
     3121 gaagatattt ctttccgaca aggaaattgg agccattact gggcatggaa cagtacgtaa
     3181 tgccgacacg ggtaatttgt tagagaaaat tcaagacgtt tggtttgatg gacaatatag
     3241 gattattaaa ggaatggaaa gttctttttc ctcattaaca tgttgttctg gatctctttc
     3301 tgcatttaga agagaagcta taataaatca tattcatgaa tgggcaaatg atagattttt
     3361 aggtatggaa tttaaatttg caacggatag aagattaacg gcatttattc ttgcagaaaa
     3421 aataaaaaat aggaatgaga aactcattaa aaaaaacccg acaaaagaga aaggaagaaa
     3481 aataaaatct caacaatgga aattaaaata tagcccaagt gtaaatgtag aaattggagt
     3541 accccaaaca ttaagaagtt taattaaaca acagatacga tggagaaaaa gcttcattag
     3601 aagtattttt gcaacaggat cgatttattg gcaaagacct ttaactgttg caattattta
     3661 ttatttaaca attgctttaa gattatttag gccttacatt ataataaaat cgcttatcat
     3721 attacctttg aatggtgata tcttgagtgg aattttttat ctgggtggaa caatgtttgc
     3781 agggttgatg tatggcattg atgtaagact aagaaagcca ggttatccta ggtggtttta
     3841 tagaccgtta atgaatgtta tttcaacatt tattcttaca tggttaattg tatatgctgc
     3901 tataacaata aaaaataaaa catggagata aagtttatta caaataatct aaataactaa
     3961 tttgaattta gataagtctg aaaattcaaa agcctttctt atgtgtaatg gtggtattta
     4021 ttcaatatta ttcaattaca atttcttcaa aatatcgcag aacagtaatt tcagacactt
     4081 gtggaattat taaattatta ttactaatca tttctaatag cattgcagga aagtttgcac
     4141 cggcaagcgt agtaaaaatt gtacctcctc caaatctagg attgacttca ataattttta
     4201 gttctccatc tttagactct tttaattgaa tacaacatgg acctttaatt cctaaggtct
     4261 ctgcagtttt tttacaaaga ttttctatca gggaatctcg ttttattttt ccttttactg
     4321 atataccagc tttagtatcc attcttattc ttggaattgc cataattggt ttttcagtaa
     4381 gatcagaaag tacgtctact gtatattcta ttcccggaag atattcctga aatatcatat
     4441 ttttaaattt tctagacaca tagtctagat ctaatttgtt ttctattttt ataatattag
     4501 tacttccttt gccaaaacgc ggtttagcaa ttaaaggaaa tgtatctatt tctttatcat
     4561 attcacaagt aaaagcaaaa ggaaatttat ttgataagaa ttcatatgat aataatttat
     4621 ccctgcaaat ttctaaaacc ttccttctgc taactattgg tattgctccg tattctacta
     4681 ttaaatcatg attgatgcta tatccaaaaa tatcaaatcc agatgaaggc atcaaaactt
     4741 taatattttg ttcgttaatt atttttaata aatttaccaa aaaaaactta ttatcatatg
     4801 ggggtataac ataataaaaa tcgctaagaa aaaagcctgc agatagtgaa tttgaatcag
     4861 ttgatataat attcccttta aatgatgaca ttcgtaaaga tttaatagta ttaattccag
     4921 ctggccctcc cgctccagga ataagtacat tgaatgtatc cattagcata gttataatat
     4981 aatgataatt aaatttaata tctgccttga ataactattc ttattatatt aaagagtatt
     5041 aaatctatta ataacttatc taatcaaggt aaattaataa tcaaattgag tgaaacagtg
     5101 atatattatt ataataaaat agagaattat atattattat aaatcattat tattagaaat
     5161 tatgaattta aaagaggatt atcatattca tactaactat aacaatcatt cagcctcaaa
     5221 tcttacagtt aaaaatgtgg tacaggaagc agaacgaaag ggattagaga taattgcttt
     5281 aacagaacat gtaagagaaa gttcagagtg gataccaagt tatttaaatg agatattaca
     5341 ctacaaacaa aaaacaaaga ttaaagtaat accaggattt gaagccaaaa ttttgtttaa
     5401 tggtaatatt gattgtagag aagaaatttc tagagattac ttcattattg caagttttca
     5461 tacgaaatat tgtgataaag tgatttggat aaatgcatta aaaaaagtaa ttgaaaataa
     5521 gcacgtaaat gtgataggcc atttagctcc ggagagttca tttgagataa atccaacaga
     5581 gatagaagaa ttagcaaaac ttcttaaaaa acacgacaaa atagtagaag tcaatgcaaa
     5641 atatcagagg ccacctgaaa cttggataaa aatatttatt aatagaggtg ttaaatttca
     5701 tcttggtagt gatgcacatt cacttaatga aattgggaat tatgaatcta tcataaagtt
     5761 aattaaatta tttgagtaat gataatcaat ttttagttca caatatataa taagaatatt
     5821 tacccttttt atagatagta tattttaacc ataaaatttt atatcactta tatcattaat
     5881 tcgctattgc ctatagtaag tatttcgcta aatgatgata ttattaatga tattgataaa
     5941 ttgcagaaga atcttggatt tactggaaga tccgaaattg tgagagcagg attaagaaat
     6001 attatagcag aacaaaaaga aagagataca ttacagggaa aattggctgc tttattacta
     6061 attattcatc aagaaagaga tgatgatcaa gttactaaca tgagacacga ttttgaagaa
     6121 attgtgacta cacatatcca tagcaaaatt gatcaagaca gatgtgttga attattttta
     6181 ctaaagggag atgcagagtt aataaggaat atgactaaaa aatttcaaac taataaaaaa
     6241 ttagaacatg taaagcttat tgctatgtga aataatatac attataaaaa aattatttta
     6301 tttttaattc taaggaatat ccgataagat ctgtatttac agttgctggg cccatataag
     6361 tgctggtgaa ttgtgcagat tgtactgaat cctgaattat agagctacag gaataataat
     6421 cgattcctga ttcaagacaa aatttgagca attcattatt aaattctgtt aaggaattac
     6481 tggagattgt atcttcatta ttactatttt tatctacgat tttggcgaaa tatttcatat
     6541 tttgattttt tacttgattt atggtaacag aagattcact agaatcttga ttctttaaaa
     6601 gagacattat ttcgctatta acgacgtttg acgtattagg caatgatgat ttattgggag
     6661 gagaggagct gggcgaggag gaacttggtg gaagtaatga actcagagga gaagagctgg
     6721 acgaggagga acttggtgga agtaatgaac tcagaggaga agagctggat acagaagagt
     6781 ttgatacaga agagtttgct gtaggtgtag aatccggagg agaagaaaca gatgatgagg
     6841 aacttggtgg aagtaatgaa ctcagaggag aagagctgga tacagaagag tttgacgaag
     6901 gaggagagcc catcccttgc attaacgatt ctatagtact atctattata tttcctcttc
     6961 ctactttatt taaaagatta tttatttctg tatcaatgtt tgttgaatta gaaccattat
     7021 ctgattgtgc ataaactaag tttactaatt gattaccgct aattacagac atcaataaga
     7081 ttaaaccata caataccaaa aatctaaatt tcatttaatt attttacctc gaccgatctt
     7141 atatctttta gtataaatat gaattgtaaa aaagttataa ttaactctcc tataatatta
     7201 tagaatccga taacgattca atacaaaata aaacaattat ttatttcctt aggaatatac
     7261 aaaaaattta taaattttaa aatatattaa aaaataactt ttcaatatat taattgaatt
     7321 attgtgttag atttcccgaa gtttgattta tttgggacat gtctaaaagt tttggagtat
     7381 caattaattc tattatacat gttttgtctg tggtttctat tattcctgta cacccagaaa
     7441 ttctatttat ttcatatcca aatgtattag atatcttaac gtcataattt cctggtttta
     7501 tagatacttt ttggaagtta ttgctgcctg caaatgaact tggtacaacg ttttctcctt
     7561 ttactgttat gataaaatct aatggttttt tggtcccacc atggacattt attacttcta
     7621 atagaacatt caagtcaagg tcacgtacaa gtgggtttat gtttggagtg tcattattgt
     7681 tactgttatt aatatactta tttgttatag cgattgtaga tgtaagattg ttacttgtaa
     7741 gattgttact tgtaagattg ttacttgtaa aattatcaat agtgtttgtt aattcattag
     7801 aggtatttgc agtatctata tcatccaatg ttactacaca ttctctgtta tcacctaaac
     7861 ctatgatacc tttacatcca gctgataaag ttgtcttata tccagtgaag ttatttattt
     7921 tcacttcata agatcctcgt tctatagata cagattgtcc atcgggatta ccagcgaatg
     7981 aatgtggagt tgcctgtgtt ccattaactg atactataaa atcttttgtt tgtttagttg
     8041 cgccattatc attaatgacc tgtgtcacta tatttatgag cgatgcagag gtaagtgatt
     8101 ctttttgaat tggattggca aatagattaa cctcatttat actggcccaa tcatttacac
     8161 tatttccagt aacagtaatt ctaaggtact taccaacggc gtctggaata tcatatacct
     8221 caatatcgtc agtagtatta ctattagtgc tggaaaaaac ctgtctaaaa tcataaccat
     8281 ctgaagacac agagactacg aaatcaatgt ttcgaatatt ccctttatac caagaaattc
     8341 caatatcaca tatttgtttt tcttgtccta gatcaaattg tatccatgaa cctaatccta
     8401 gattagacca ttttgtggaa agattattgt cgactaaatt gcctttagtg ttattaagtg
     8461 aagtgtctat tgacgatata tgattaactg ggatatttaa ggggaaacac ggagaaggag
     8521 agccaggaat tatgggatta ttaatatcaa tctcagatat actagaccaa tcattcattg
     8581 aattgtcatt aacggtgatt ttaaggaagc gggttttaac atccggaata tcgaattttt
     8641 cagctccatt ggtaagacca ctgctagttt cagaaaatag attgctaaaa atttctccat
     8701 ctgcagatgt agatatagtg aatttaacat tcctatgatc acctttatac caagagatat
     8761 caatatcaca tatctctttt tctttaccta aatccaattg agtccaagaa ttaagtccat
     8821 atttagacca ccgtgtagat tgattaccat caattgcatt gagttctgta ttattgccta
     8881 catgtcctat ggaggtaaca ttaacagctt tttcaggttt acaagaggta ttatcaccaa
     8941 atgacacaaa tactaattga ttatctagat tgaatttgtt ttgtgtatat gcatcatata
     9001 tctctgcagt ggtaagaggt ctatcccaaa tacgaacttc atccacgtcg cccacatata
     9061 acttgtcttt gaagagagag ttcgcagcaa tggcaatcct attttttcca gttatgtcag
     9121 gtttcgcatg ctttgtattt aagctatgat caaacacacc atcaataaac aactttagta
     9181 atgatccatc gtatgttact gtagcataat gccatttatt gtcattaact ggttttttag
     9241 aagtaacaaa ataatttgag gagtcttttg cttcaaatcc tgcttgaaga tgccctaggt
     9301 aagttatcca aaatccataa ttcatatttt caccagacgc ttcggaacca aatccacctt
     9361 tattaactac aaatgaaaca tggggatcag tattatttgt tttgaaccaa acagcaattg
     9421 aaaattgatt caattgaaat aattctgaat ctttaatata tttaaaatta tgacctgtaa
     9481 aggttccaaa ttttccaatt tgtgcttcta cactattcat ttgaaaaaga tttgaagaaa
     9541 aaatagtaaa tgaaattaga aaagaaatta agattaatct aatcatttta ttttcataaa
     9601 ttttataact tgttgtcatt atcttctcac caagaattag tcaaataaat ttcttttatc
     9661 actttcagaa atttaccaag atgtcctata atatgtttta ccaaaaaatg ttgataaaaa
     9721 aataaaataa atttactcat tataaaatgt taaataataa taactatctt tcacacaacc
     9781 agacatatta ttctcttttt tttcccaatg ataacaaaac ttctttacca aaatcagaag
     9841 caagtttttt ttcttccatc attccgcact caatacactt cactgtatta taagatttca
     9901 cggtatcacc tccacagata taacatatac aatatactat accgaaatct ttatcgctta
     9961 tagtagcatg agcagaagag ttcaacaaac taaagattct tcctcttact atatctccaa
    10021 ccctatacat gaatttaggt cttctctcac ctctgtctct tctggaattc catcctcctg
    10081 attctgaggc tatccttgtt gaggcaattg ctgatagacc tgaagaaaat tttttattgt
    10141 tgagatacaa gattcgtata gaaatcatac taccaaataa catttctata tatccaacta
    10201 taacatcacc tatctttgga aagtgcgctt gttttattct ttctattgca acttgtcttt
    10261 ttttaaaatc atattctttt ttccctaaac ttagagattt tatagaacca tctacaacaa
    10321 aagtattttt cccactttca aattcttcta taaacgcaac atgttcacca ggaaatataa
    10381 tatcataatt cgcttttttt ttaatcattt aagaattgta tatatcttaa agttataatt
    10441 gttatttttt tagcctcttc tagtttggtt atagatagta taaccctctt gctaggctta
    10501 atttaatagt aattgatata ttaatactaa aaaaaaaatt ccaaccaaaa ttaaactgat
    10561 tccagcagca aaaattattc ggaaattacc ttttcttaat tcttttttta aattatcaaa
    10621 tttaaaaaac tttagcaatt ctctcaatat ctattaatag aaagcctact tcaaacagta
    10681 atcttattac tgctctctta tactttctaa ctttgacact acttgccaaa gagttgttct
    10741 gatatgtgat tccatatgtc tattttgcgt taagctatcg agcatactaa ctgcattagc
    10801 ggctctaaca gatatactcc catttttttc ttcttttaaa atatcataaa tatcttttat
    10861 ttggttgcgt ataacccttg gaagatcttt attctccttg aaagattcca aagttgcaat
    10921 tgtactctca agtctatctg tattttcttt attacggttt tctttttttg atttcataat
    10981 agcaaactaa acctcgattt tatggagaat aaaaattact aaattattac ttatttatat
    11041 gtttttaatt ctcatccatt taaataaaca aaataaaaac ataaaaagat ctgctgtata
    11101 gattaaatat tactaaatct ttattatttc tatgcaatgt gcagtttatg atacatatgt
    11161 aacaaaaaaa gatggaaaaa ttatgcattt tgatgtgatt gtagaag
//
LOCUS       DASVEV010000179         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1631076, whole genome shotgun sequence.
ACCESSION   DASVEV010000179 DASVEV010000000
VERSION     DASVEV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631076"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VF222_08250"
     CDS             <1..1037
                     /locus_tag="VF222_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HEX7142454.1"
                     /translation="IAPPSDVSNLDLFLDQLKEIDTLLIDPIKIKRKKNFKIIYESEL
                     ADIIICKSIEDLISNKIKGKFLAYYKKVFSNEDIHDIESAIYNGANYIIVDAANWKII
                     PLENIIANLQNTRAKIFTTAKNSEEVRTMFAILELGVDGVILQTANVKEIEESKQYLK
                     DKKFEIKVCEVVGFRDIGIGERVCVDTVSMLETGEGMLVGSKSNFLFLLHNESAGSSF
                     TSPRPFRVNAGAVYCYTILPNGKTTYLSELESGTEIYIVNKEGNSRLVSVGRCKIETR
                     PLRLIKAIGNNTEGTVIVQNAETIQLIRDTGELLPVTSIKQGDKILGYFKPSSGRHFG
                     VEVEEYILEK"
     gene            1191..2060
                     /locus_tag="VF222_08255"
     CDS             1191..2060
                     /locus_tag="VF222_08255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7142455.1"
                     /translation="MQIKHSYVDKILSGDCKEIISKIPNGSIQLTITSPPYRNAIDYS
                     AHTSKNGSYYRGKSNINTKEYLEEMIDIFNNKIYNATKEGGYCCIVIANEVVNGTLLP
                     LPHMVLSQLVEPFGKWNLHEEIVWHKVTGGTNRYGSFVINPYPKYYRANIMHELILVL
                     RKGNVSSGRTERTETLPATHEEWTKEIANSVWHIAPIPPGYIEHPCPFPEEIPYRLMK
                     LYSYEGDTILDPFNGSGQTTKVAFHLQRRFIGVDIINEYVKLARSRLIEPIHIRAEAL
                     IANWKKIPSHHNL"
BASE COUNT          823 a          294 c          378 g          720 t
ORIGIN      
        1 ttattgcacc tccatctgat gtttctaatc tagacttatt tttggatcag ttaaaagaaa
       61 ttgatacgtt acttatagat cctattaaaa taaaaagaaa gaaaaatttt aagattattt
      121 atgaatctga actggctgat attatcattt gcaaatctat agaagattta atctcaaata
      181 agataaaagg aaaattcttg gcttattata agaaagtttt ctcaaatgaa gatattcatg
      241 atatagaaag tgcaatctat aatggtgcta attatattat cgtagatgcc gctaattgga
      301 agattatacc attggagaat ataattgcta atttacaaaa tacaagagca aaaatattca
      361 ccaccgccaa aaattcggag gaggtaagaa caatgtttgc gatattagaa ttgggtgtag
      421 acggggtgat tcttcaaact gcaaatgtta aagaaataga agaatccaaa caatatctaa
      481 aagataaaaa atttgaaata aaagtatgtg aagttgtagg ttttagagat ataggaatag
      541 gagaaagagt atgtgtagat actgtttcaa tgcttgaaac aggagaaggg atgttagttg
      601 gaagtaaatc aaattttcta tttcttttac ataatgaatc agcaggctca tcttttactt
      661 ctccccgtcc ttttagagtt aatgctggag cagtatattg ttacactatt cttcctaatg
      721 ggaaaactac atatctttcc gaattagaat ctgggacaga gatatatatt gtaaataagg
      781 aaggaaattc tagacttgta tctgtaggga ggtgtaaaat cgagactaga ccattgaggt
      841 taattaaggc aattggtaat aacactgaag gaaccgttat agtccaaaat gcagaaacaa
      901 ttcaattaat ccgggacact ggagaattat taccagttac ttccattaag caaggagata
      961 aaattttagg atactttaaa cctagtagtg gaagacattt tggagtagaa gttgaagaat
     1021 atattctgga aaaatgaata tttcttttat ggactcgtat aattgttaat acaaattaaa
     1081 aatgttacaa atctcatcta ctcaagaatt gttattgtat aaaatttctt tattagttag
     1141 tcgattattt taaagcatgc taaatattga ttaaaaattt attttcaatt atgcaaataa
     1201 agcatagtta tgttgataaa attttatcag gcgattgcaa agaaataatc tctaaaattc
     1261 ctaacggttc gattcagttg actataactt ctcctcccta tagaaatgca attgattata
     1321 gtgcacatac ctcaaaaaat ggatcatatt atcggggaaa atcgaacatt aatacaaaag
     1381 aatatcttga ggagatgatt gatatcttta ataacaaaat atacaatgca acgaaagaag
     1441 gtggttactg ttgtatagtt atagctaatg aagttgtaaa tggtacgctc ttaccacttc
     1501 cccatatggt cttatcacaa ttagtcgaac catttgggaa gtggaatctt cacgaagaga
     1561 tagtttggca caaagtaacg ggaggaacta atagatatgg ttcatttgtt attaatccct
     1621 acccaaaata ttatagagca aatatcatgc atgaactgat tttagttctt agaaaaggga
     1681 acgttagtag tggaagaact gaaaggacag aaacattacc tgcgacacat gaagaatgga
     1741 caaaggaaat cgcaaattct gtatggcata tcgctccaat tcctccaggc tatatagaac
     1801 atccatgccc ttttcctgaa gaaattccat ataggttgat gaaattatat tcttatgagg
     1861 gagatacaat tctagatcct tttaatggta gtggacaaac tactaaagtg gctttccatt
     1921 tacagcgccg atttattgga gtagatataa ttaatgaata tgttaagtta gctcgatctc
     1981 gattaatcga accaatacat attagagcag aagcactaat cgcaaattgg aaaaaaattc
     2041 cttcgcatca taatttataa atatattact atttacttta gattcaatta tttttatcct
     2101 tcattagtta tacgttttaa tatttagata ataattactc ataaagtatt catgcaatta
     2161 aaaaatttat atttatttaa ataaatgtat tagtgaatag ttttactttg tcagg
//
LOCUS       DASVEV010000180        12015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1047396, whole genome shotgun sequence.
ACCESSION   DASVEV010000180 DASVEV010000000
VERSION     DASVEV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047396"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VF222_08260"
     CDS             complement(<1..662)
                     /locus_tag="VF222_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142456.1"
                     /translation="MERNNIKLHTHKVEKMKDATIHIVPHMHYDAVWILNKEDYYFVN
                     IEYILKQAINLIKKFDYKFMLEQTFLLEKIEKDYPHIFADISDLVKDNKIEIAGGQYL
                     LADNMVPNGEVLIREITEGKKFVKEKLNKDIVVSYGADEFGFNAQWPQILRNSGYKYF
                     IFRRGINISTPSEFFWEGLDGTQILTHWMPLGYRAGLFLDQLSLSLKQLKKYASTNQI
                     LM"
     gene            complement(623..1474)
                     /locus_tag="VF222_08265"
     CDS             complement(623..1474)
                     /locus_tag="VF222_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7142457.1"
                     /translation="MSQYLIFSDIDNTLLQSYIIKEGEIYHIEEYGEIKEIVQKLRKN
                     NIPLVLCSSKTRFEQEKIRKYLEINDPYVVENGGAIFIPENYFPIDLEEIGLSINRNN
                     ENLVIELGKSYNRIIETLQEIREIFHIEFIAVHDLSLPELAQKVGISIEDARLMASRE
                     YSETILEININKLDKLKKILNDRGLRILQGTRYNTVSSLHDKGSAISILKKLYSIKYE
                     NEKIESIGIGDAFNDISMFENVDKPFLVRNIQNSYAPMKINNLIRVNGIGQTGWKEII
                     LNYILTK"
     gene            complement(1551..2108)
                     /locus_tag="VF222_08270"
     CDS             complement(1551..2108)
                     /locus_tag="VF222_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7142458.1"
                     /translation="MDIHEYYSILGVTEDATLVEIKKSYRFLVKKYHPDRNSGKAIDS
                     EIIKKINIAFEVLSDKHKRLEYDDLCSEIQNYDINHTMEKKTTLRNYNEGIENTTTPS
                     RIISKEKSRFRIIVEPSLCMAFGSCEILAPNVFHVETNKIINPKAVVVSETGNNFESI
                     LDAAQTCPTKAIIIIDRDTGKQIFP"
     gene            complement(2205..3161)
                     /locus_tag="VF222_08275"
     CDS             complement(2205..3161)
                     /locus_tag="VF222_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7142459.1"
                     /translation="MNSIELMKKEIEQQVEKLKITDFEKSKKSSEYLFVGSGDSFVSG
                     LIASYESNFNCLCMDPTEIICNPTLSKNKRVCFISLSGKTKENILAAKIVKQYCKETI
                     AITANPTSDLAKNCMKVINLDYQKPPLATAGTLGFILTTMTALALVKKSKLVINTKKV
                     YEKAEELSNDIIDKTKKYLPFTKDHSIFFLGTSTLFPISCYGSLKINEVLGLKSSAFS
                     VEYFCHAPLFSLTHKDILLIFSSKNQHMNKKANKLNYLLNKQKFYSFLIEIPFNTNVE
                     ILFFSSLFVQLLVYGLAKEKKLKNCYFLENKELLEISSNLIY"
     gene            complement(3580..3708)
                     /locus_tag="VF222_08280"
     CDS             complement(3580..3708)
                     /locus_tag="VF222_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142460.1"
                     /translation="MEITWTTPKPTKEITHFKISFMGKNKNNKKHLDYIFAIKGNE"
     gene            4279..5034
                     /locus_tag="VF222_08285"
     CDS             4279..5034
                     /locus_tag="VF222_08285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7142461.1"
                     /translation="MNLKNKVAIITGAGGTVGRDITKSLISEGCKVVINGRDAKKLNN
                     LSKEIGNKKDILILPGDITKEGDVLNMIDQITNNHNKIDILVNNAGVINDPKSFHLTT
                     QDEWMNLINVHIIGTLQMTKAVLPIMVESRDGCIINISSTLGTRAIPGVPLSVYGLTK
                     GGIISFTKHIAVEYGPYGIRCNCIVPSTIRTPFLEPFMKDENAKKTLESSFPLRRIGE
                     PSDISNAILFLCSDKSKWITGAEINIDGGITAK"
     gene            complement(5392..6288)
                     /locus_tag="VF222_08290"
     CDS             complement(5392..6288)
                     /locus_tag="VF222_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7142462.1"
                     /translation="MEFVDTSIIEQIEMKMIRPSSFAVRDKFQKYCEDDESLIISIRE
                     HGLIQPILIRPLSHGFEIVAGHRRYQACKSLRWRFIPCKIREMTNKQAFEIQLSENIQ
                     RKSMDPIEEAEAFRRYVIDFGWGGVSDLAKKIGKSEEYVSHRIQLLKLSDEIKKKIAS
                     NRLNVSKAIEISTIPIEKQSQIVGEIIENNLSVKRIRELKMILRDNINEDYSNIGIAN
                     GNNYISKSLKITKKASLSLKVTLARIDNLIEESQISIEPEQRTEIINFLMQIRIRIHE
                     LIDDTIKFKNNNKKIFRSKKKY"
     gene            complement(6534..7424)
                     /locus_tag="VF222_08295"
     CDS             complement(6534..7424)
                     /locus_tag="VF222_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7142463.1"
                     /translation="MDFLLQPFHYEFFNRAILVSILVGGTCGLLGVYIVLRGMSYIGH
                     GMSHAVFGGAISSYIIGLNLYIGAAIWGILSALLITEINNRYKIKADAAIGVVTTAGF
                     AIGIFLMSTAQTPVRNFEALLFGNILSVNDLDFIFITFVSIVTITFIFIFQKRLLFTI
                     LDTDTAKVYGVKTKRVDLLFSIVLAIVVIAAMNSIGVTLLAVAIIAPAISARLITNNF
                     LKVLTLSAVIGIITAFLGMYCSFYVDSPSGATIVLFGTASFIIAVAYSHIKKSYHMHF
                     HGDKKHSHPHSHTEDHRHEH"
     gene            complement(7460..8197)
                     /locus_tag="VF222_08300"
     CDS             complement(7460..8197)
                     /locus_tag="VF222_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX7142464.1"
                     /translation="MNDIKPSEIILTLKNVSFGYNYHLPVIDGITLDIPRGRFIGLLG
                     PSGSGKSTLLKLIVGLYRPWDGTIKFENFSNKKNKKSTIIGYSPQVESIEWDFPVTVR
                     EFVSMGLWNRSGFFPWVNKISRIQIDNLLHELGICDYADRHIKELAGGEQKRVFLARA
                     LIHNPEILVLDEPTSGLDKTNRENFLQILSDLNLKGMTIIMTTHDIDGVAKKLPWIVC
                     LNKNIISQGEPTSTLSDNILYRTYDLK"
     gene            complement(8253..8717)
                     /locus_tag="VF222_08305"
     CDS             complement(8253..8717)
                     /locus_tag="VF222_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7142465.1"
                     /translation="MDDGIKKSIETIPLENRKLLTYHDSWAYFASRYGMKVIGAIQPS
                     DFGEPSPKDVANLIEQVQIQNVPSIFASEGFPTNIIEQIAKEGKVTIVETLRDDDLPG
                     NPGDYNHSYIGMMLENMKNMIVPLGGNIDALKDISPQNILQPKFPFLYIKYK"
     gene            complement(8929..9297)
                     /locus_tag="VF222_08310"
     CDS             complement(8929..9297)
                     /locus_tag="VF222_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7142466.1"
                     /translation="MNNASVIISKSFLLIGMFLFFISLKPSYASEQDNSRQQIGEKLN
                     VVTSVAPITNIVHNIDGDKINLVGLVPEGINSHTYEIITSNVLKIHKGNLIIIDGLTL
                     EAGIEKLPIIYSLKIQIYSY"
     gene            9970..11067
                     /locus_tag="VF222_08315"
     CDS             9970..11067
                     /locus_tag="VF222_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEX7142467.1"
                     /translation="MSNNAILDFFYILFNGISEVPLFSSPITGILILAGVFLASRSAG
                     VMMVISGLIGAGVAILLGAPYGLVAFGLFGYNSILTGMAFWSGPFVKSNKATFFISVF
                     GAAVTAVVWMAMAHLMGDMFILNGLQESWAIPGFTSSFIFTTWAIMYATKRFGHDIWP
                     EPIPSKTEELIKGSGNPSQINEADFKWTATEFIKATLKGVSQVTFIENWKTGVFWVVG
                     LTLAFELAPILTGQAKPWWTNAFTAQWNEFSPLYLGGVMALTGSAVGVAMAILAKLPT
                     AETRAGLHGFNQVLVMIALTSFIPLTPQAFMIALFAVISCSFVMPALQRFFGMWGLPA
                     LTGPFVFTTWMFLLGISGFTNIPAGIGWARP"
     gene            11101..11694
                     /locus_tag="VF222_08320"
     CDS             11101..11694
                     /locus_tag="VF222_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142468.1"
                     /translation="MEFDDFKEFLDFLNHNKKDNENSVDNLLYFIEWCNNNNHEELLV
                     RLNSEEKGGLVNNSVIDFTTNRIIITRKKIFNRIFELGFVAGLGPFPYMILSKKKQQS
                     KIKDSAKLQIEKILNDQNLECTINYNDILKIVIKRGSNSVVRNMFGSFITKNYLTIST
                     KNNNKYEYILPVKKNGEFRKMSYWMKSILPVEISLID"
     gene            11807..>12015
                     /gene="ureA"
                     /locus_tag="VF222_08325"
     CDS             11807..>12015
                     /gene="ureA"
                     /locus_tag="VF222_08325"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX7142469.1"
                     /translation="MLVPREIDKMMIWTAAQIAEGRKKRGVKLNHPESVAYIANFVVE
                     GARDGTSVADLMKSAREVLTRNDVM"
BASE COUNT         3985 a         1829 c         1728 g         4473 t
ORIGIN      
        1 aacattaaaa tttgattagt tgaagcatat ttttttaatt gtttcaacga aagggatagc
       61 tgatctaaaa aaagtccagc acgatagcct agtggcatcc aatgagtaag aatttgagtc
      121 ccatcaagtc cttcccaaaa aaattctgat ggagtagaaa tatttattcc tcttctaaag
      181 atgaagtatt tatagccaga atttctcaaa atttgaggcc actgtgcatt aaaaccaaat
      241 tcatctgcgc cataagaaac tactatatct ttatttaatt tttcttttac aaattttttt
      301 ccttcggtta tttctcttat tagtacctct ccatttggaa ccatgttatc agctaacaaa
      361 tactgtcctc ctgctatctc aattttatta tctttaacaa ggtcacttat atctgcaaaa
      421 atatgaggat aatccttttc tattttttct aacaaaaaag tttgctctag catgaattta
      481 taatcaaatt ttttaataag atttatagct tgttttaaaa tgtattcaat attcacaaag
      541 tagtaatctt ctttgttaag aatccacact gcatcataat gcatatgggg aacgatatga
      601 atagttgcat ccttcatttt ctctactttg tgagtatgta gtttaatatt atttctttcc
      661 atcctgtctg tccaattcca tttactctga taagattatt tattttcatg ggtgcgtaac
      721 tattttgaat atttcgtaca agaaatggtt tgtcaacatt ttcaaacatt gaaatgtcat
      781 taaatgcatc tcctattcct atagattcaa tcttctcatt ttcatatttt attgaatata
      841 atttctttaa aattgaaatt gcagagcctt tatcatgtaa agaagaaact gtattatacc
      901 tagttccctg taaaatacga agacctctat cattcagtat tttttttaat ttatctaatt
      961 tattaatatt aatttcaagt atagtttcgc tatattctct tgatgccatt agtcttgcat
     1021 cctctattga tatgcctacc ttttgagcta gttctggtaa tgaaagatca tgtacagcaa
     1081 tgaattcaat atggaaaatt tctcttattt cttgaagagt ttctattatt ctattgtatg
     1141 attttcctaa ctctataact aaattttcat tgttacggtt gattgataaa ccgatttctt
     1201 caagatctat aggaaaataa ttctcaggaa taaaaattgc tccaccgttt tcaactacat
     1261 atggatcatt gatctctaga tattttctta ttttttcctg ttcaaatcgg gtttttgacg
     1321 aacataatac caaaggaata ttgttcttgc gtaacttttg aacaatttct ttaatttccc
     1381 catattcttc tatatgataa atttctcctt cttttataat atatgattgg agaagcgtat
     1441 tatctatatc agaaaaaatc aaatactgtg acattctttt tactttactt tcttaaaaga
     1501 atgaaatact ttatacttta acgtaagaaa ttatataaga attttctata tcatggaaaa
     1561 atttgttttc ctgtatccct gtcaataatt atgattgctt tggttgggca tgtttgtgca
     1621 gcgtctagaa tagattcaaa attgtttcca gtctctgata ctaccactgc cttgggattg
     1681 ataattttat tagtttcaac atgaaaaaca tttggagcta aaatttcaca acttccaaaa
     1741 gccatacata acgagggttc aacaataatt ctaaatctag atttttcttt agaaattatt
     1801 ctacttggtg tggtagtatt ttctataccc tcattataat ttcttaatgt ggtcttcttt
     1861 tccatagtat ggtttatatc ataattttga atttctgagc ataaatcatc atattcaagt
     1921 ctcttatgtt tatcggaaag aacctcaaaa gcaatattaa ttttttttat tatctctgaa
     1981 tcaattgctt taccagaatt tcgatcagga tgatattttt taactagaaa ccgatatgac
     2041 tttttgatct ccactagtgt cgcatcttcc gttactccta aaatgctata gtattcatga
     2101 atgtccaatt aataatgata ataaacttca atggatattt ctttatttag attgagattg
     2161 ctccaaaact tttgacaatc gtcttcttct ctatagaaat tcgtctagta tattagattg
     2221 gagcttattt caaggagctc tttattttca aggaaataac aattttttaa ctttttttct
     2281 tttgctaacc cgtaaactaa taattggaca aagagggacg agaaaaataa aatttctaca
     2341 ttagtattaa aaggtatttc aataagaaat gaataaaatt tttgcttatt taacagataa
     2401 tttaacttgt tagctttttt attcatatgt tgatttttgg acgaaaatat caataatatg
     2461 tccttatgtg ttaaactaaa taatggtgca tggcaaaaat attcaactga aaatgcagat
     2521 gattttaatc ctaatacttc atttattttt aatgaaccgt aacaggaaat tggaaataaa
     2581 gttgaagtac caagaaaaaa aatggagtga tctttagtaa aaggaagata tttttttgtt
     2641 ttatcaataa tatcatttga caattcctca gccttttcgt atactttttt agtattaatt
     2701 accagttttg atttctttac taacgctaat gctgtcatgg tagttaatat aaaacctaaa
     2761 gttcctgctg tagctaaagg aggtttttga taatctaaat taattacttt catacagttt
     2821 tttgcaaggt cgctggtagg attagcggta atcgctattg tttctttaca atattgtttt
     2881 actatttttg cagccaaaat attttctttt gtttttccag aaagagaaat aaaacatacc
     2941 cttttattct tacttaaagt tggattacat attatttcag taggatccat acataaacaa
     3001 ttaaagttag actcataact tgctattaaa ccagaaacaa aagaatcacc agaacctaca
     3061 aaaaggtatt cggaagattt tttacttttt tcaaagtcag taatttttaa tttttctact
     3121 tgttgttcaa tttctttttt cattaattct atagaattca tattgactat ctaatatgat
     3181 aataaacttt aaataaatat tatagtaaaa acttgattta attcagatat tatttttgct
     3241 ttaaaaaata ttatttacat aaattttcct gatacttgaa agataaatag aattgtaaaa
     3301 tatcttacat ccttatcaag actaataaat ttctaagtat aaatcataat ttacaaatca
     3361 ctcagttagt aatagaaaaa aagattttaa taataaaaat attgcctatg tcttatttat
     3421 tattattcac atacaaattt tactttctgt gtatatatag gttgaaatga tattccaatt
     3481 attgttaatt ccaaaaacaa aatttgtgac tggttaattt ttaattgggt catttctatt
     3541 ccccatcctt agtgaatttg gatattttga gcaattaatt tattcattac cttttatagc
     3601 gaaaatataa tccagatgtt tcttgttgtt tttattttta cccataaaac taattttaaa
     3661 atgagtaatc tcttttgtag gcttaggagt agtccatgta atttccacat cgatatcttt
     3721 gagttccttt ttttgatttt aatattttct gtaacatcca cattcctttc atccattgat
     3781 attgagggga ttgaaatgta tatttaccaa ttgtttttaa cttaaatgaa aagctattgt
     3841 taggatcgat tttataattt tttttgtaac attattttct gttaatatct ctattaatgt
     3901 ggaatattat ctgcattact cccctttacg gtttcttttg gctttaatgg ataatatgtg
     3961 atgtcatgga acctctctct tttttcgtca cttgaactga gaataatggt tatgatttct
     4021 tcagcctgta ggatcgtgaa atactcttgc tgttataaaa taaacagaga gagactaaaa
     4081 acgatggcga taattgcaat ctattaatat tatttattaa atattgcaat agttaatttt
     4141 tatgctaagt atacatgaat tttttttaat actataatta ccataattat ttcacttttt
     4201 ttcaggcttg ataaacgtaa aaaattgaat aaaaagagat cgatttaaaa tgatgtaatt
     4261 aatactacat agatgtatat gaatttaaaa aataaagttg ctattataac tggagctggt
     4321 ggaacagtag gcagagatat tacaaaatct ctaatttctg aaggttgtaa agttgttata
     4381 aatggacgtg atgctaaaaa actaaacaat ctctcgaagg aaataggtaa taaaaaggat
     4441 atcttgattc ttccaggaga cataacgaaa gaaggtgatg ttttaaatat gatagatcaa
     4501 attacaaata atcataataa aattgacata ttagtaaata atgcaggagt tataaatgat
     4561 ccaaaatctt ttcatctgac aacccaagat gagtggatga accttatcaa tgttcatatt
     4621 ataggtacac ttcaaatgac aaaagcagtg ctaccaatta tggttgaaag tagggatggt
     4681 tgtattatta atatttcttc gactttggga acaagagcaa tccctggagt gccattgtca
     4741 gtctatggac ttactaaagg aggtataatt tcatttacca aacatattgc tgtagaatat
     4801 gggccgtacg gtatcagatg taattgtata gtaccctcta ctattagaac tccttttcta
     4861 gaacctttta tgaaagatga aaatgcgaag aaaactctag aatcatcttt tcctcttcga
     4921 cgaattggtg aaccatctga tatatctaac gctattttat ttctttgttc tgataaatcc
     4981 aaatggataa caggagcgga aattaatata gacggaggca taacagcaaa ataattattt
     5041 attttgtatt atcgatattt gatgattcaa aagtattttt atgtaatatc tcattaaata
     5101 tgtgtctata ttattttgat cattatgttt ttaattagcg gtaaccaaat aacattccat
     5161 ttgaaatcaa atctctccta ccttctgaat attcaacaaa tatagcacta acaagtttat
     5221 actatagtaa tactttgtat attccaaatt ttaagtaata caatgtaaca cttgtatcaa
     5281 acaatgactt gtacaaataa gaaatataaa aaaaatgtta cggtaatggt atatatcgtt
     5341 cttcatgcaa taacatttct atgtccaata aaagactaaa tactttgatt gttagtattt
     5401 ttttttactc ctaaagattt ttttgttgtt atttttgaat tttatagtgt catctatcaa
     5461 ttcatgtatt cttattctta tttgcattaa aaaattgata atttctgttc tctgttctgg
     5521 ctcgatagat atttgtgatt cttctattaa gttatctatt cttgccaaag taactttgag
     5581 agacagagat gccttttttg tgatctttaa ggattttgag atataattgt taccattggc
     5641 aatacctata ttactataat cttcattaat attatctctt aaaatcattt ttaattctct
     5701 tattcttttt actgaaagat tattttctat tatttctcct actatctgtg attgcttttc
     5761 aattggaata gttgagatct caatagcttt actaacgttt aacctgtttg aagcaatttt
     5821 tttctttatt tcgtcggaaa gcttaagaag ctgaatccta tgagagacat attcctcact
     5881 ttttccaatt ttcttggcaa gatcacttac acctccccac ccaaaatcaa taacatatct
     5941 tctaaatgct tctgcctctt cgataggatc cattgatttc ctttggatat tttcagagag
     6001 ctgaatttcg aatgcttgtt tatttgtcat ctctcgaatt ttacaaggaa taaatctcca
     6061 tctcaaagat ttacaggctt gataccttct atgacctgct actatttcaa atccgtggga
     6121 taacggtcta ataaggatgg gttgaattaa tccatgctct ctaatactaa taataagtga
     6181 ttcatcatct tcgcaatatt tttgaaattt gtctcttact gcaaatgatg aaggacgaat
     6241 catcttcatc tctatttgtt ctattatact tgtatcaaca aattccacag taaagaatag
     6301 tggagtatgt ttatatttat tatgatttta ttcatttatt tttattataa aatcttattt
     6361 attataaaaa ttatctctct ttaatcgatt tatcattatt ttcattacat aaatatactc
     6421 ctattaaatt tttcacaatt ttcgagaatt aactatgctt tataaagtaa gatagagctt
     6481 atccaaaaga aaaaattcta ttttttaatc atttaaaata taataatatg tgatcaatgt
     6541 tcatgtctat ggtcctcagt atgactatgg ggatgagagt gttttttatc tccatgaaaa
     6601 tgcatatgat atgacttttt aatatgtgaa taagccacag ctataataaa tgaagcagtt
     6661 ccgaataata ctatggtagc tccagagggt gaatcaacat agaaactaca atacatgcct
     6721 aaaaacgcag ttattatccc gataaccgct gaaagggtta atacttttaa aaaattattt
     6781 gtaattaatc ttgctgatat tgcaggtgct ataatagcta ctgcaagaag tgtaactcct
     6841 atagaattca ttgctgctat tacaacaata gcaagcacta tggaaaataa caaatccact
     6901 ctctttgtct taactccata aactttagct gtatcagtat ctaatattgt aaacaataat
     6961 ctcttttgaa aaatgaatat gaaagtgatt gtaacaatag agacaaaggt gatgaaaata
     7021 aaatctaaat cattaacaga taatatattt ccaaataata gagcttcaaa gtttctaaca
     7081 ggagtttgag ccgtactcat taaaaaaatt ccaattgcaa atcctgcagt tgtcactaca
     7141 ccaatagccg catcagcttt tattttgtat ctattattta tttcagttat taataaggct
     7201 gataaaatac cccatattgc tgctcctata tacaaattta atcctataat atagctactt
     7261 atggctccac caaagactgc atgggacatc ccatgtccga tatagctcat tcctcgtaat
     7321 actatataca cacctagtaa tccgcatgtt ccccctacca gtatagaaac taaaatagct
     7381 ctgttaaaaa attcataatg aaatggttga agtaaaaaat ccatagattg ttacactcct
     7441 attatttctc aataatcagt cattttaaat catatgtcct ataaagaata ttgtctgata
     7501 aagttgaagt tggttctcct tgtgaaataa tgtttttatt gagacaaact atccatggca
     7561 actttttagc tactccatca atatcatgag tcgtcataat tatagtcatt cctttcaagt
     7621 ttaggtcgct caatatctgt aagaaatttt ctctattggt tttatcaaga cctgaggtag
     7681 gttcatctaa aacaagtatt tcagggttat gaatcaatgc tcttgccaaa aatacccttt
     7741 tttgttctcc acctgctaat tctttgatat gacgatctgc ataatcgcag atccccaatt
     7801 catggagtaa attgtctatt tggattctgg aaatcttatt tacccacgga aaaaaaccgg
     7861 accggttcca taatcccatc gaaacaaact ctcttacagt aacaggaaaa tcccattcta
     7921 tactttcaac ttgaggtgaa tatcctatta ttgtagattt tttatttttt ttgttactaa
     7981 aattttcaaa ttttattgtt ccatcccatg gtctgtataa gcctacaatc aatttcaaaa
     8041 gagtagattt acctgagcca cttggaccca ataaaccaat gaatctacct ctgggtatgt
     8101 ctaatgttat accatcaata actggcaaat ggtaattata accaaacgaa acatttttca
     8161 aagttaaaat gatttcagag ggttttatgt cattcattta cattcatttg cagtttatct
     8221 tttgttatta aaatgagata ataatctttt attcacttat atttaatata taaaaaggga
     8281 aacttaggtt gtagtatatt ttgaggacta atatctttca atgcatcaat attcccacct
     8341 aaagggacta tcatattttt catattctct aacatcattc ctatatacga gtgattgtaa
     8401 tcgcctggat tgccaggtag atcatcatcc cttaatgttt caacaatagt aacctttcct
     8461 tctttagcta tctgttctat tatgttggta gggaatccct cagaagcaaa tattgacggt
     8521 acattctgaa tttgaacctg ctctatcaaa ttcgcaacat ctttaggact tggttcacca
     8581 aagtctgatg gttgaatagc tcctattacc ttcattccat aacgactcgc aaagtaagcc
     8641 catgaatcgt gataagtaag caattttcta ttttctaacg ggattgtttc aatggatttt
     8701 tttataccat catccaattt tttagtaatt ctgtatattt gttcgaattt tcattgtaat
     8761 attcggaatt agatgggtcc aattctatta atttatcacg aggtaaattt gcgaacttta
     8821 tcgcataatc tacatccaac caaagatgag gattaggatc accgttccct attggaaaac
     8881 taaagtcaaa tatcctattt tttttgggta ttgttttttc tccaagtttt agtaactgta
     8941 tatttgaatt tttagagagt atattattgg caatttttca atacctgctt ctaaggttag
     9001 cccatctata atgatgagat ttcccttatg gatttttaat acattagaag ttataatttc
     9061 gtatgtatga gaatttatgc cctcaggaac taatccaacc aaattaattt tatcaccgtc
     9121 aatattgtga actatatttg ttataggggc aaccgaagtt actacattta gtttctctcc
     9181 aatttgttgc ctactattgt cttgttctga agcataggat ggcttaagcg aaataaaaaa
     9241 aagaaacatg ccaattagca aaaaagattt acttataata acagaagcat tgttcataaa
     9301 ttgattattt tatttgacct attggtatta tataaacagt aacttattta gtaagtattc
     9361 gtaagtgatt taaaaagaaa tatctcgtaa cttcagcaat aaccgaaagc atttttaaat
     9421 tgatccatga agcgattgat ggtaatcgag aattttatta ttttgatata atgatataat
     9481 aaatgagata agacctttga gacttttaat tactctgcct tcattaataa tgtgacaaaa
     9541 ttggtgattg ttatgtcttt ttgtctaata tctgtagttt cgagacattt gtgttggtcg
     9601 agatataaaa gcgtagtaga tccattagca tatagacatt tttcattaat tattaaatga
     9661 acaaataatt taagaaggaa atgatatact gattcttctt gtagaagatt taagatctat
     9721 ccaactattt tcaactcaac atatatcgat ttaattactc ttaaaatcat tcataaaaat
     9781 ttcttcattt gcttttaatt caaaaacaaa taatgatgga tttgtcatta tttttgttct
     9841 aaaataaaat ttcattcgta gaaaaaattg atagttagat tagacttttt ttattaataa
     9901 agatatttga tttacaaatc aataaagaat acatcatgat tttgaattat catggtagta
     9961 gcagattttg tgtctaacaa cgctattttg gactttttct atatcttatt caatggaatt
    10021 tctgaggttc cactatttag ctcccccatt actggaatct tgatactcgc aggagtattt
    10081 ctagcttcaa gaagcgctgg tgtaatgatg gtaatatcag gtttaatagg tgcaggagtg
    10141 gcaattcttc ttggagcccc atatggacta gttgcttttg gattgtttgg ttataattct
    10201 atactaactg gtatggcttt ctggtccgga ccttttgtta aatctaacaa agcaacattt
    10261 ttcatatctg tatttggtgc tgctgtaact gctgtagtct ggatggcaat ggcacatctt
    10321 atgggagata tgtttatcct taatggatta caagaatcat gggcaatacc tgggtttact
    10381 tcttccttta tctttaccac ttgggcaata atgtatgcta caaagagatt tgggcatgat
    10441 atatggcctg agcccattcc atcaaagact gaggaattaa ttaaaggtag tggaaatccc
    10501 tctcaaatta atgaagctga ttttaaatgg actgcgaccg agttcattaa ggctactctg
    10561 aaaggggtat cccaagtaac ctttatagaa aactggaaaa ctggagtatt ttgggtagtt
    10621 ggacttactt tagcatttga gctagcgcca attctaacag gtcaagctaa gccatggtgg
    10681 accaatgcct ttacagccca atggaatgaa ttttcacctc tctatttagg aggtgtaatg
    10741 gcacttactg ggtctgcagt aggagtagca atggccatct tggcaaagct tcctacagcg
    10801 gaaacgagag caggattaca tggatttaat caagttctcg taatgattgc cttaacaagt
    10861 ttcataccat taacccctca ggcctttatg attgcactat ttgctgtaat atcatgcagc
    10921 tttgtaatgc ctgccctaca gcgatttttt ggaatgtggg gtttacctgc attaacaggg
    10981 ccatttgtgt ttacaacatg gatgttctta cttggtattt caggatttac aaatatccca
    11041 gctggaatag gatgggcaag gccataaatt cttattccaa gaacccatta tttttttgaa
    11101 atggagtttg atgattttaa agaattctta gactttttaa atcacaataa aaaagataat
    11161 gaaaatagtg tggacaattt attatatttt atagaatggt gcaacaacaa taatcatgaa
    11221 gaactcttag ttagattaaa ttctgaagaa aaagggggac tggtaaataa ttctgtaatt
    11281 gattttacta ctaaccgtat tattataaca agaaaaaaaa tattcaacag aatatttgag
    11341 ttgggttttg ttgcaggact tggcccattt ccctatatga ttctgtctaa gaaaaaacaa
    11401 caatcaaaga tcaaagattc agcaaaatta caaatagaaa aaattctaaa tgaccaaaat
    11461 ttggaatgta ctattaacta taatgatata ctaaaaatcg ttataaaaag aggatcaaat
    11521 tcagtagtac gaaatatgtt cggttccttt attacaaaaa actatttgac aatttccaca
    11581 aaaaataata ataaatatga atatattctc cccgttaaaa agaacggtga atttcgtaaa
    11641 atgtcgtatt ggatgaagtc tattttacct gtcgaaatct cattgataga ttgactagcc
    11701 gacgtcggct aatcagataa atcatgtatt tccatttata taatttaact atttgaaatt
    11761 taaaataatc cacatccagt aatatttcag gtgaattgga aatttgatgt tagttccacg
    11821 tgaaattgat aagatgatga tctggactgc agcgcaaatt gctgaaggca gaaaaaaaag
    11881 aggagttaaa cttaatcatc ccgaatctgt agcatatata gcaaattttg ttgttgaagg
    11941 tgctagagac ggaacaagtg tagcagattt aatgaaatca gctagagagg ttttaactag
    12001 aaatgatgtt atgga
//
LOCUS       DASVEV010000181         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1241699, whole genome shotgun sequence.
ACCESSION   DASVEV010000181 DASVEV010000000
VERSION     DASVEV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241699"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF222_08330"
     CDS             <1..625
                     /locus_tag="VF222_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142470.1"
                     /translation="EEQQPLSKNDPVQISLTNPATIKSKLLSDGGLYDITVILEKTSK
                     GIKIESDKQINLFISIGKTFPFVIKESADSRSNNNNKGNLTLQVKTFYDEIQNFSFNK
                     ENSKISFKMPFKWDLNYVNQILTLHEEIIVPKSYTPLSTVSSFIGTLNGMKIPTNTIL
                     IDDYSDKNNRIVHVVITTFKLKEYAKQIVEEGGNSYAIFELEPVKTV"
     gene            820..1467
                     /locus_tag="VF222_08335"
     CDS             820..1467
                     /locus_tag="VF222_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142471.1"
                     /translation="MKSTIRSIILGLNVLLVLAIATTVYNSQFLLNNNSIMAYAQQIM
                     GMPPGGMGMEQQQQPGPGQYAGGTISSIQNDEKGTPAWILSGLWKGALTNTADKSNQD
                     FSTPTVSNNNDNLPTATFEAEFDMVMLNGSELHQHSIYNFTLADIMKIDDANYQVNGT
                     ATVTMKDGPVNNVPLSLKSMNNNVISIWVDPTKVNNHFGNTPIFGLIQQDIVIKK"
     gene            1816..2370
                     /locus_tag="VF222_08340"
     CDS             1816..2370
                     /locus_tag="VF222_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142472.1"
                     /translation="MSQKHKTWVCTICSQSFTRNSSAKRHDINLHEGNADYVRYIDYE
                     IGRIQGKYYQNDPALYKKKSYNYNNLTTNKQSLNISSERTKLSLDDHKYFNLNSFSHS
                     NNDESSQTASFFDKPKEIMELVDQINTIGKNVWPTYLMNQAIIWLLSPYLFNSPNPKT
                     TVEKYLNDFKEITERKRLSNLFKS"
     gene            2715..>3548
                     /locus_tag="VF222_08345"
     CDS             2715..>3548
                     /locus_tag="VF222_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142473.1"
                     /translation="MVNSDSSHTKRNPLIFPYKWIALGNTTLGTLIASLDANIIFIAL
                     PIITSELGASLFTMVWIVIGYSLVTASILLNFGRLADIFGRVKLYTLGFIIFTLGSAL
                     CSISQTGEQLVVFRIFQAVGAAFLFSNSVAILTDAFPSNERGKALGINQIAIVLGSVL
                     GLVLGGFLTSYLGWRSIFWINIPIGIIGIISSYKNLKELGSIKKEKIDWLGNISFAGG
                     LFLVLVGITFSSFGIISDFLVIFCIIFTGLALLIIFGFIEKVISKDYPMFHFSLFKIK
                     SF"
BASE COUNT         1325 a          525 c          508 g         1190 t
ORIGIN      
        1 agaagaacaa caacctttat caaaaaatga tccagtccaa ataagtttaa caaatccagc
       61 tacaattaaa agtaaacttc tctctgacgg tggattatat gatataacag ttattcttga
      121 aaaaacaagt aaaggaatta aaatagaatc ggataaacaa attaatttat ttataagtat
      181 aggaaaaact tttccttttg taataaaaga gtctgcagat agtagaagta ataacaataa
      241 taagggtaat cttactttac aagttaaaac cttttacgat gagatacaaa atttttcttt
      301 caataaagaa aattcaaaaa tttcatttaa aatgccattt aaatgggatt taaattatgt
      361 aaaccagatt cttactttac acgaagagat catagtccct aaatcttaca cccctttatc
      421 tacagtttcg tcatttatag gtacactgaa tggtatgaaa attccaacaa acacaatatt
      481 aatagatgat tattctgata aaaataatag gattgtacat gttgttatta caacttttaa
      541 attaaaagaa tatgctaagc aaattgtaga agaaggagga aattcatatg ctatatttga
      601 attagaaccg gttaaaacag tataagtaat actctactat tacaacttcc tatatcacaa
      661 agtttgaaaa acattcattg ttgactgttt aataatataa gataatcgta tgtataaaaa
      721 aatagccggt aaagacacaa ctaactatta tttttactta ccaaccactt taaaaaaatc
      781 taaattatta tatataacat atattctttg tttagtactt tgaaatctac tattagatcg
      841 attattttag gattaaatgt attactggtc cttgcaatag ctactactgt ctataattct
      901 caatttcttt tgaataataa cagtataatg gcctatgcac aacaaattat gggaatgcct
      961 ccaggtggaa tgggtatgga acaacaacaa caaccaggcc caggccaata tgctggggga
     1021 acaatttcca gtattcaaaa tgatgaaaaa ggtacacctg cttggatatt atccggccta
     1081 tggaaaggtg ctttaactaa tactgctgat aaatcaaatc aagatttctc gacaccaact
     1141 gttagtaata ataatgataa ccttccaacc gctacctttg aggcagaatt cgatatggtg
     1201 atgctaaacg gatctgaatt gcatcaacat agcatctata actttacact tgctgacatt
     1261 atgaagatag atgatgcaaa ttatcaggtt aacggaactg ctacagtaac catgaaagat
     1321 ggtccagtca ataatgttcc actcagtcta aagtcaatga ataataacgt cataagtatt
     1381 tgggttgatc caacaaaggt aaataatcac tttggaaata caccaatctt tggactgatt
     1441 caacaagata tagtgataaa gaaatagtct aattgttgtt aatataaata cttcatcata
     1501 ttaacaacta taactactat tatcactatt aatcattttt ttttaaaaga taagttaaaa
     1561 aaagatcgta attatttaga gtagaattat cagataatag ttacaataac cgttctttaa
     1621 attttcttac gacagattca aagttggatt gattcgaata aaattttgaa gtagtaaatg
     1681 taaacgatat acaattaaat ttttcaaatt ccaaacaatt tattattttg cccaggtttt
     1741 cttagcatac aaaatcttat ttagagtaac acagtgttac acttttaaga gtaatctatt
     1801 atttgtatta tatacatgag tcaaaaacat aaaacctggg tctgtacaat atgctctcag
     1861 tcatttacaa gaaactccag tgctaaacga catgatatca atctgcatga gggaaatgca
     1921 gattatgtca ggtacatcga ctatgaaata ggaagaattc aaggaaaata ttatcagaac
     1981 gatcctgctc tttataaaaa aaaatcttat aattataata atcttactac taacaaacaa
     2041 tctctaaata ttagctcaga aagaaccaag ttgagtctcg acgatcataa gtattttaac
     2101 ttaaattctt tttcacattc aaataatgat gagtcatcgc aaacggcttc tttctttgat
     2161 aaacctaaag aaatcatgga gctggtagac caaattaaca ctataggaaa aaatgtatgg
     2221 cctacatacc taatgaacca agccataatc tggttgttaa gcccttatct ttttaatagt
     2281 cctaatccca aaacaacagt agaaaaatat ttgaatgatt ttaaagaaat aacagaaaga
     2341 aagagattat caaatctatt taaaagttaa aaaatttatt aatcctctta taatcagtag
     2401 gagatagcta aaacaagctt cccattatta aaaataagat taagggctcg aacttatata
     2461 atatcagatt tgaaatagca gataactatt ataactctaa gaattttcac atttcaaata
     2521 cactttaaac gactgctatt ttcattcttt gaaagataac aaaatgcatt taactttaac
     2581 atctgaaaga gtttataata aaataaattt attgaaacac aattttgata tgaacataaa
     2641 gattatggta ttgtaatatc ataagaatga gaaagaatag atatcatata ctttattaac
     2701 taaagatcgg ctgattggta aactcagact catcacatac taagagaaat ccattaattt
     2761 ttccatataa gtggatagcg ttaggaaata ccacgcttgg tacccttatt gcatcacttg
     2821 atgcaaatat tatttttata gcattgccta tcattacatc agaacttggt gcttctcttt
     2881 ttacaatggt ctggatagta atagggtatt ctcttgttac agcatctatt cttttaaatt
     2941 ttggtcgtct tgcagatatc ttcggtagag taaagctata cactttaggc ttcattatat
     3001 tcactttagg atctgcatta tgcagtatat cacaaaccgg tgaacaatta gtagtattta
     3061 gaatttttca agcagtaggt gcagcatttc ttttttcaaa tagtgtagca atcttgacag
     3121 atgcatttcc atcaaatgaa aggggtaaag ccctaggcat aaatcaaatt gctattgtgt
     3181 taggttcagt actcgggctt gttcttggag gattcttaac ttcatacctt ggttggagat
     3241 ctattttttg gatcaatatt cccataggaa ttattggtat catttcatct tataaaaatc
     3301 tcaaagaatt aggatcaatc aagaaagaaa aaatagattg gcttggaaat atttcatttg
     3361 caggtggatt atttcttgta ctagtaggaa ttacatttag ctcatttggt attatatctg
     3421 attttttggt aattttttgt attattttta cggggttggc tcttctcata atatttggtt
     3481 ttatagagaa agttatttcc aaagattatc ctatgtttca tttttcactt tttaagatta
     3541 aatcattc
//
LOCUS       DASVEV010000182         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_660643, whole genome shotgun sequence.
ACCESSION   DASVEV010000182 DASVEV010000000
VERSION     DASVEV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660643"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(642..887)
                     /locus_tag="VF222_08350"
     CDS             complement(642..887)
                     /locus_tag="VF222_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142474.1"
                     /translation="MTTLFEQKEIEKGKQLKSEEKSENYFSNAITFYKHLLQCRSCSW
                     NVSYSEYSGSLDISDDLVFCPVCKDGKIESSKYRQYR"
     gene            1213..2091
                     /locus_tag="VF222_08355"
     CDS             1213..2091
                     /locus_tag="VF222_08355"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7142475.1"
                     /translation="MAQTTKTKYVHPEVLVDTQWVEEHLENKNVRIVEVDYDPKSNYN
                     LGHIPNSILIDWKKDVNDPITRDILSLNNFQELLQHNGINNNTTVILYGDFNNWFAAF
                     AFWVFKYYRYHDVRLLDGGRKKWLEEDRPISKETVNYPKGNYELEEEIKPNDEIRAFL
                     GQVKNALVSRRYFQLSLVDVRSGAEYRGEITAPPEYPTEGAQRGGHIPGAKNVPWTQV
                     INDDGTFKTIEEIRKLYDEQGITQDKDIITYCRIGERSSHTWFVLKYLLGYPNVKNYD
                     GSWTEWGNLIGNPIEK"
     gene            2358..3344
                     /locus_tag="VF222_08360"
     CDS             2358..3344
                     /locus_tag="VF222_08360"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7142476.1"
                     /translation="MLDQLSDKRVDYKKPRHQGITYIIDKFQGFDKENFSIISPMIDI
                     VKIYGAYPLFIPEEQLIDRIKFYHENNVLVSTGSTLTEYAITENVFEQFVKETAALGF
                     DIIEIGENNLQINAEQKKKISNIIGSYGLEHHWKIGKKDPRRQLTTEMSINKINEALN
                     ITKDKVILEANLGYSVGIYDEKGNIKWNILSAIVSNVSFDNIIFEAPLEVQQAALIAE
                     YGERVNLAEIKIENVASIESLRRGFLSKASFSISTARKGADGGPATKFIYFIIQNKSP
                     IEQSELIHLSSLARRTVQASIDDLKEQGLIYETNSLDDIRRKVYHLAKDGSV"
BASE COUNT         1459 a          494 c          575 g         1243 t
ORIGIN      
        1 tatgttttat tccttacaac taattaatca atttccctca caataaatat ggatttatct
       61 ttcttgttat aagtttagta gaagggaaaa catgtgcata cttcgctaat attactactc
      121 cttaaccatt cctcctccac cattagtaac ttaatttcat taaagtaatg caattataat
      181 tgatgataaa tttaccaagt tttaattttt tattcttttt tatttttact taaaatgaat
      241 aaatttaatt ataactatat ctatattatt aattatttta ttctttagat ctaaaattat
      301 ttataataag gctaatccta ttcatgtgta attttccttt agagtgtatc aagaggcatg
      361 cataaagcag atctatatta tgttactaac acctagtaga gatatcaatt gatataacgg
      421 ttaaaatagg attttgattt gtattctaac ccacgattat aaaaaaacta aactgattaa
      481 aatatattta aagtcctaat tgaattgata ttggttagat gcagtatatt tatatttatt
      541 ttaacatata ctcctaatta tagtatttag gataatttat ttttaaatta tactttatta
      601 aaattgtttt aaaacaagaa gatccccacc gtgaacagat tttatctata ttgccgatat
      661 tttgaagatt caatttttcc atctttacat acagggcaga ataccaaatc atcagatata
      721 tcaagagatc cagaatattc tgaatatgaa acattccacg aacaagacct acattgtaag
      781 agatgtttat aaaaagttat agcatttgaa aaataatttt cacttttttc ttcgcttttc
      841 aattgctttc ctttttcaat ttctttttgt tcaaacaaag tagtcataac gattaatata
      901 tatatattta ttataagtaa tataatgaaa tctaaatatg caatttagaa atattatgtg
      961 ctctttttgc atattaacta tcgaaaatca ttagatgaat aaagataaaa tcttatatca
     1021 aaatcttatc agtcttctga tttctaattt acaataataa ttaaacttat aacaagaaag
     1081 ataaatccat atttattgtg agggaaattg attaattagt tgtaaggaat aaaacatact
     1141 ttattttaca cataatttaa ataaattacg catatcatag caataatata tcatacatta
     1201 caattaatta ttatggcaca aactacaaaa acaaaatatg ttcatccaga agttcttgtt
     1261 gatacacaat gggtagaaga acatcttgaa aataaaaatg taagaattgt tgaagtagac
     1321 tatgacccaa aatcaaatta caatctaggg catataccta actcaatatt aattgattgg
     1381 aaaaaggatg tcaatgatcc tataaccaga gatatattaa gcctaaataa tttccaagaa
     1441 ttacttcaac ataatggaat taataataat actacggtta tcctatatgg agattttaat
     1501 aattggtttg cagcatttgc tttttgggtt ttcaaatatt atagatatca tgatgttaga
     1561 cttttggatg gaggaagaaa gaaatggcta gaagaagata gaccaattag caaagaaaca
     1621 gtaaattatc ctaaaggaaa ttatgaatta gaggaagaaa tcaagcctaa cgatgaaata
     1681 agagcatttc tcggtcaagt taaaaatgca ttagtaagta gaagatattt tcagttatca
     1741 ttagtggatg taagaagtgg cgcggaatac agaggtgaaa taacggcacc tccagaatat
     1801 cccacagagg gtgcacaaag aggaggtcat attccagggg caaaaaatgt accttggact
     1861 caagtaataa atgacgatgg aacatttaaa accatagaag agatacgtaa attatatgat
     1921 gaacaaggaa ttacccaaga taaagatatt ataacatact gtagaattgg tgagcgctct
     1981 tcacatacct ggtttgtatt aaagtatttg ttaggttatc caaatgttaa aaattatgat
     2041 ggatcatgga cggaatgggg taaccttatt gggaacccta ttgaaaaatg atatggtgat
     2101 aaattataca ataaaattta attttgtata acatatactt attataaaga tgtactcaaa
     2161 tttctaatgg gatcataaca caaattcact attattcaag ctcgcctagt gcttcattaa
     2221 gcatacctag ttttttggta taatttatat gcaaaattta cacaatacat gaaagttttg
     2281 tttataatta ctattcttca atgaataaat gaatatatca aaatatcaat ataggtaaaa
     2341 catttatcta tattatcttg cttgatcaat tatctgataa aagggttgac tataaaaaac
     2401 caagacatca aggaattaca tatataatag ataaattcca aggatttgat aaagaaaact
     2461 tttctattat atctcccatg atagacatag tcaaaatata cggcgcttat cctctcttca
     2521 taccagaaga gcaattaatt gataggataa aattctatca tgaaaataat gttttagtat
     2581 ctactggaag cacgctgaca gaatatgcca ttacagaaaa tgtgtttgaa caatttgtaa
     2641 aagaaacagc agcacttggg tttgatatca ttgaaattgg agaaaacaat cttcaaataa
     2701 atgcagagca aaaaaaaaaa atttctaata taattggatc ctatggatta gaacatcact
     2761 ggaaaatagg aaaaaaggac ccaaggagac aattgaccac agaaatgtca attaataaaa
     2821 taaatgaagc gttgaatatt acaaaagata aggttatttt ggaggcaaat ttaggctata
     2881 gtgttggcat ttatgacgaa aaggggaata ttaaatggaa tattttaagc gctatcgtat
     2941 ctaatgtctc ttttgataat attatatttg aagctccttt ggaagttcaa caagcagcat
     3001 tgatagctga atatggtgag agggtcaatt tagcagaaat aaaaatagag aatgttgcat
     3061 cgattgaatc actaagaagg ggatttttgt caaaagcaag ctttagtata tctactgcaa
     3121 ggaaaggggc agacggtgga ccggctacaa agtttattta ttttattata caaaataaaa
     3181 gtccaattga gcaaagcgaa ttaatccatt tatcaagtct agcaagaaga acggtccagg
     3241 cgtcaattga tgatttgaaa gaacaaggat taatatacga aacaaatagt ctggatgata
     3301 ttagaaggaa agtatatcat cttgcaaaag atggatcagt ttgatcatat tggttcaagg
     3361 atataatgcg atgacagttt ggtttgttat gtctccgtga ataccatgta catctacaat
     3421 gtagaaaagg ccttctaaaa tcttgttgtt gaatgactat taaaacatca aagtagtaat
     3481 cgggatgtcc agcttcttta ttgtaaaata accacgaata gggtattttt gaggcaaata
     3541 atcctaaaca gtcttttttt taagaacttt tagaggactt gctaggcact tttttagatt
     3601 tgagcaaata ttttcaatct atcagaaata cccactgtaa tcaatataaa ttacagtttt
     3661 gtaaaagggt ctatatgcca aagggtaccc ctaaagagtc agatataaac aaaaaaccca
     3721 ccaaaaatta taaaagacaa tatttattca tagagattag tgagacggaa a
//
LOCUS       DASVEV010000183         8039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_661209, whole genome shotgun sequence.
ACCESSION   DASVEV010000183 DASVEV010000000
VERSION     DASVEV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661209"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..652
                     /locus_tag="VF222_08365"
     CDS             206..652
                     /locus_tag="VF222_08365"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7142477.1"
                     /translation="METLNNSKIARLATVDPENHQPYIIPVVFVFNGTNIFIPIDDKP
                     KKTVNSNQLKRVKNIQKNPNISFLIDTYDDEDWNNLSYLMIQGKARIVVNRLKDIDTI
                     QKVHSLLSEKYFQYKKLVGIGDSCIVIDIQKVIKWNYSRKKNSNSY"
     gene            807..1733
                     /locus_tag="VF222_08370"
     CDS             807..1733
                     /locus_tag="VF222_08370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX7142478.1"
                     /translation="MKVGLVVPQFGINATKSNLMRFVQIAEREGFDSLWVYDRMLYAI
                     NPQQGYGGTPDKREWPEYFKNSLDPLTTLAFIAANTSKVKLGTCIIDMVFHNPLTLAK
                     EFTTIDILSEGRVICGFGIGWSKDEYLAANIPYEKRGERTDEILQAMKKIWTDDIVEF
                     NGNFYKIPKSIIGPKSIQKPHPKILLGGFSPKTFERMIKYGDGHIGVLVGSFEYFTNS
                     IKMFNDSIEKSSRTRKDFDLTILTYPYLMKSSSEGDRAPMTGRTIDEIGSDLSMLKSS
                     GVDRVILAVNADKDYDVNETIEFVKELRKSCQ"
     gene            2332..2745
                     /locus_tag="VF222_08375"
     CDS             2332..2745
                     /locus_tag="VF222_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142479.1"
                     /translation="MAFLLGPILRKVSLSTRKEIISWLMPTMVFYMPTVASVTTTADY
                     FLASKMGLITLELPVVYWITTVLIIVTVMLIQGLGILLPTNIRVYHELRKKEPDMTKI
                     QRLMRRYVKVVATQAILQFVIIFIMANFATGFFFR"
     gene            complement(2852..3808)
                     /locus_tag="VF222_08380"
     CDS             complement(2852..3808)
                     /locus_tag="VF222_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7142480.1"
                     /translation="MSKVNNQKIIKEMERQAVTLDADNSVNNKSIRSVLRIIKSETTQ
                     DGEGVTLNRSFPNKFISEFDPFLLLDEMGPVDIKPGKQKGFPDHPHRGFETVTYLLEG
                     KFEHKDSQGHAGTISAGDVQWMTAGSGVIHSEMPEKEFSKNGGRLHGFQLWVNLPKSN
                     KMMKPQYQEIPQSKIPTGTTKDGNLTVKVIAGESLGAKAVIDTITPIMYLHFKLEPGA
                     RIIQPVPKEYNVFAYVIKGKGVFEQSNNNKTAEKGNLVIFEKDGQAAYIQAIEDSNVS
                     LELLLIGGVPLREPIARYGPFVMNTQQEIYQAIEDYRSGTLG"
     gene            3974..4108
                     /locus_tag="VF222_08385"
     CDS             3974..4108
                     /locus_tag="VF222_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142481.1"
                     /translation="MIFFLENDIELRIVNLLKSNEGPPERFDPERNFNNKQLANERLS
                     "
     gene            4192..4398
                     /locus_tag="VF222_08390"
     CDS             4192..4398
                     /locus_tag="VF222_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142482.1"
                     /translation="MTSLQVYGQEPTTTTNSITDKIKTTSVQIKNTASEVVNNSTGEI
                     NQTEAKNTLNQLGEAAKKNSIRGS"
     gene            complement(4602..5123)
                     /locus_tag="VF222_08395"
     CDS             complement(4602..5123)
                     /locus_tag="VF222_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7142483.1"
                     /translation="MTMPKGWKNEQTTISKKEKENSNEQTEQSSSKNGQTSSTITVDD
                     KIIIQELIAKHNVALDNKNIDEWTNSWTDDGIWSTTFGEAKGKTELKNLINQVTNEFA
                     SGKRHLSTNIIIEGAPNNTASAQSYLIVTEAKKTPEVVASGTYTDTLKKDASGKWKFL
                     QRKLDIDLVNDSK"
     gene            6081..6443
                     /locus_tag="VF222_08400"
     CDS             6081..6443
                     /locus_tag="VF222_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142484.1"
                     /translation="MIISLERSLEENIYEGIKTLEYHIRPQNYPIVNKMLQLQIKTLQ
                     WVVNKQKKNSLESLKQIVNFKVKRLEYELKIARGDIEHTSKIVYQLEMLECCKIMINW
                     DLQRMTKITKEDDVSAFC"
     gene            6723..6875
                     /locus_tag="VF222_08405"
     CDS             6723..6875
                     /locus_tag="VF222_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142485.1"
                     /translation="MFSNHPIGHEIDYRKNDEIRHKKRGSYQNDLIPRKRKCEQCSTE
                     IWYSIL"
     gene            complement(6982..7200)
                     /locus_tag="VF222_08410"
     CDS             complement(6982..7200)
                     /locus_tag="VF222_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142486.1"
                     /translation="MEKYINSNWKSGNFFKTCLDKTKDNKDTYDNYLNNIKKLMKYKD
                     SNDDDKDSIYIFITSSNPFFSSSWLRGL"
     gene            complement(7419..>8039)
                     /locus_tag="VF222_08415"
     CDS             complement(7419..>8039)
                     /locus_tag="VF222_08415"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7142487.1"
                     /translation="ETAITTEDTIEEEATSPTDNETAITTEDTIEEEATSPTDNETAI
                     TTEDTIEGKPSELLEMDPEAEDEIQDIEDQQTEQAQQEEDEAQTPQVEEESPNTEPTA
                     RDDSVTASQNQPLNIKLNANDVDGDNLSFTIVDDPSNGQLGNVNPSTNTVTYTPDEDY
                     SGEDNFTFIVNDGTIDSNDATVSIIVEEGEQTEQAQQEEDSQQEED"
BASE COUNT         2765 a         1133 c         1218 g         2923 t
ORIGIN      
        1 caaaaaacaa aataataaac ggaatatttt caaaggtaaa agtatacata atttagttat
       61 atatgacaaa aatgtttttt taacttttac tccaaatacg ttatttttaa atgaatgttt
      121 aatttttctt ctatagttaa gacttatgta gttcaataat taaaataatt ataatagtaa
      181 tttgacaaaa ggcagaagcg aaatagtgga aactttgaat aattcaaaga tagctagatt
      241 agcaacagta gatcctgaga atcatcaacc ctatataata ccagtagtat ttgtatttaa
      301 cggtactaat attttcattc caatagatga caaacctaaa aaaactgtaa attctaacca
      361 attgaaaaga gtaaaaaata tacaaaaaaa tcctaatatt tcttttctaa ttgatacata
      421 tgatgatgaa gactggaata atctttctta tttgatgatc caaggtaaag caagaatagt
      481 agtaaatagg ttaaaagata tagatacaat acaaaaagtt cattcactat tatctgaaaa
      541 atattttcaa tataaaaaat tagtaggaat tggagatagt tgcattgtaa ttgatattca
      601 aaaggttata aaatggaatt attctaggaa aaaaaatagc aattcatact gactacttgt
      661 ttagtattta gaaaaggact tgagttgtaa ataatattat tcaaatatga taattcacga
      721 gtactatatg gaataataag atgaatctgt aaagatcata ttgttgatta tattatgtct
      781 ttatagtata aaaaataata gtaaatatga aagtaggatt agtcgttcct caatttggga
      841 taaatgcaac caaatcaaat ctaatgagat ttgtgcaaat tgcagaaaga gaagggtttg
      901 actcactttg ggtatatgat cgaatgttat atgctatcaa tcctcaacag ggatacggtg
      961 gaactcccga taaaagagaa tggcctgaat attttaagaa tagtttagat ccattgacaa
     1021 cattagcatt tatcgcagct aatacttcaa aggtaaaatt gggaacttgt attattgaca
     1081 tggtattcca taatccttta acccttgcta aagaatttac tacaatcgac attttatctg
     1141 aaggtagggt aatttgtggt tttggtattg gatggtcaaa agacgagtac cttgcagcaa
     1201 atatccccta tgagaaaaga ggagaaagaa cagatgaaat tcttcaagct atgaaaaaga
     1261 tatggacaga cgatatagta gaatttaatg gaaattttta caaaattcca aaatcaataa
     1321 ttggtccaaa atctattcaa aaacctcatc ctaaaatttt gctaggggga ttttcaccaa
     1381 agacgtttga gcgtatgatc aaatatggag atggacatat aggagtttta gtaggatcat
     1441 ttgaatattt taccaattca attaaaatgt ttaatgattc tattgaaaaa agttcaagaa
     1501 ctagaaagga ttttgatcta actattctaa catatccata tcttatgaaa agtagctctg
     1561 aaggtgatcg ggctcctatg acaggaagaa ccatagatga aatcggttct gatttatcta
     1621 tgctcaaaag ttctggagta gatagagtaa ttcttgcagt gaatgcagac aaggattatg
     1681 atgtaaatga aactattgaa tttgttaaag aactaagaaa gtcctgtcaa tagttataaa
     1741 atacaatttt tatcaattat tcatttattc aagaattagt gatattattg ctattcaatc
     1801 attttattac tatacaatta gttcttttca tcttttaaat ttatactatt gtgacgccca
     1861 tcacaaaagg ggtttaattt tttgaagctc cacatctaca taatgctatt ccttgaatat
     1921 ttgatagttt tttacctttt gaattttcaa gatttattaa gataagagag tgtcttctcc
     1981 tgtcttataa gagaatgttt ggagaatatg tgactgcaat cagatttgac cacataataa
     2041 aagaaatatt actgaaaaca tcatttgata atctatttag aagcataaca gttattatat
     2101 gtacgagtta ttgatttaac ataaataaaa ggttatatta ataagttatg caagatagca
     2161 tattaaattg gagtcttcaa tacaaaaacc cccgacaata tcattacgct caaacatatg
     2221 tattgttatt ccaattggcg ctttaattct agcgatagtg actctaatct tctacttctg
     2281 aactatgttc acgtatttac ttcaatatta tggactggaa cagatatctt tatggcattc
     2341 ttgcttggtc ccatacttcg aaaggtaagt ctttcaacac gtaaggaaat catttcatgg
     2401 cttatgccaa caatggtttt ttatatgcca actgtagcat ctgttacaac tactgcagat
     2461 tacttcctcg cttcaaaaat gggtctgatt actctagaat taccagtcgt atattggatt
     2521 accacggtat taattattgt cacggtaatg cttattcaag gacttgggat tcttcttcca
     2581 actaacatta gagtctatca tgagttaagg aagaaggaac ctgatatgac caaaattcag
     2641 agacttatgc gtagatatgt caaagttgtt gcaactcagg caattcttca gtttgtaata
     2701 atatttataa tggcaaactt tgctactgga ttttttttca gataataata gacgaattta
     2761 ttgataattt aaagtaacgt taaaaatttg gtaaaaagtt aatatcgaat ttttcctcct
     2821 ttgacttctg atggaaaatt gagaagatat tttatcctaa tgttccactt ctgtaatctt
     2881 ctatggcttg atagatttct tgttgagtat tcattacaaa tggtccatac cttgctatag
     2941 gctctcgtaa tggaactcct ccaattaata ataattcaag agaaacattt gaatcttcaa
     3001 tagcttggat atatgctgct tgaccgtctt tttcaaatat taccaagtta cctttttcag
     3061 ctgttttatt gttattactc tgttcaaaca ctccttttcc tttaattaca taagcaaata
     3121 cattgtactc ttttggaact ggctgtatta ttctagcacc cggttctaat ttaaaatgca
     3181 aatacataat aggagttatt gtatcgatta ctgcttttgc acctaaggat tcacctgcta
     3241 taacttttac agtaagatta ccatctttag tagttccagt tggaattttt gattgaggaa
     3301 tttcttgata ttgtggtttc atcattttat tactttttgg gagattaacc cataattgaa
     3361 atccatgtaa tcgtcctcca tttttactaa attctttttc aggcatctct gagtgaataa
     3421 cgccagatcc agcagtcatc cattggacat ctccagcact aatagttcca gcatgacctt
     3481 gtgaatcttt atgttcaaat tttccttcga gtagataggt gacagtttca aaccctctat
     3541 ggggatgatc aggaaatcct ttttgctttc caggttttat atccactggt cccatttcat
     3601 ctaatagtag aaatggatca aactctgaaa tgaatttatt tggaaatgat ctatttaatg
     3661 ttacaccttc gccatcttga gttgtttcac tctttattat tcgtaaaact gaacgaatgg
     3721 atttgttatt gacagaatta tctgcatcta atgtaactgc ttgtcgttcc atctctttta
     3781 ttattttttg attgttgact tttgacatac tataatatac aattgaaaat atttaaacca
     3841 agtagtaatt taatactaag taggtattta aaatcttact tgagagggga aattatgtga
     3901 acaattacaa ataggtacaa tactattcgt tttatatatc gagcaataga tactggatat
     3961 ttcttattct tgtatgatct tctttctgga gaacgacata gaattacgaa ttgttaatct
     4021 gctaaagagt aatgaaggtc caccagaaag gtttgatcct gagagaaatt ttaataataa
     4081 gcaactggct aacgagagat tatcctaaat atttcttatt acattatcaa tgaacacaat
     4141 aaaatcagta attgaatcaa tatttcttgc aatggtattt gttttttcta tatgacatca
     4201 cttcaggtat atggacaaga accgacgaca acaacaaaca gtataacaga taaaataaaa
     4261 actacttcag ttcaaataaa aaatactgca tcagaagttg taaataattc tacaggtgaa
     4321 atcaaccaaa cagaggcaaa aaataccctc aatcagcttg gagaagctgc taaaaaaaac
     4381 agcattaggg ggagctgatg tagtatctaa ttttagtggt gagattaaag agggtttaaa
     4441 gtagaaagtc aattttttat ctttatattt tttaattata gaaaagtatc ggtatactaa
     4501 tgcattgaag actaatttct gaaattttct taaatattta cactagattg actttaaatt
     4561 ttggatttag taggagataa aaagaggagt tagactctca attatttaga atcatttacg
     4621 aggtcaatat ctaatttacg ttgtagaaat ttccatttac cacttgcatc cttctttaat
     4681 gtatcagtat aagttccaga tgcaaccact tcgggagttt tttttgcttc tgttactatt
     4741 aggtatgatt gtgcgctagc cgtattattt ggcgcacctt caattataat gtttgttgat
     4801 aaatgtcttt taccacttgc aaattcgtta gtaacttggt taatcaaatt ttttagttct
     4861 gtttttcctt ttgcttcacc aaaagttgta ctccatattc catcgtcggt ccaactgttt
     4921 gtccactcat ctatattttt attatcaaga gcaacattat gtttagcaat taattcttga
     4981 ataattattt tatcatcaac agttattgtt gaacttgttt gtccattctt tgaactactt
     5041 tgttctgtct gttcgttaga attttctttt tcttttttag aaatggtagt ttgttcattt
     5101 ttccatcctt taggcattgt cataggtgtc aattaacaca actgatattt aatttaaaag
     5161 aattattatc attttagaaa aataaatagg gattgattaa taattacttt attcaattta
     5221 tatattttag atattccggc atagattggt atatgccggg aaaggtgttg aatgacttaa
     5281 tcatgtccat gccaatgcat ttccaagtac ctaacctaag atttaactat atagttaatt
     5341 ctaaaagatg aaaatttgtg gataaattct atttttaact actcaatata tttgaaaata
     5401 atgatttaat taaataattg ttttaaatat aacataaaaa ttaaaaaagt taattatcat
     5461 agtctggaca acacaacaaa tggctggaga atggatctcc agactatgac agactttgcc
     5521 aatggttttg attatgtaag tctatattta attaaatgaa ttaatattta tttattcatc
     5581 aatttctcat tctcgactat gattatgttt gttccaccat tttttttggt ttctatacct
     5641 atttctttgg tagatgttga tattgcccaa tatttctggt tagccagtat tccagtgaat
     5701 atcattgttg acgaaaatat agcatttgtt gaacatttat actattttaa ttgcaataca
     5761 aaagatttgt aataagattt attccttcaa acaagtatat gtaaacaata atttttgctg
     5821 attaatttga ctacaaaatt ggatcaaaat cagttgacat acacccatta taaatcaaga
     5881 tagaacaaca attgtgtaaa tatcagttga cacttctagc aaaaagttcg gtgtctttta
     5941 tgaaaatttt ttatgtgtac agtatagaat ttttaacaat aaattcaatt tagactcaat
     6001 tatatatatt caaatgtaat cctctccccc tctattaaaa gtgatttcct ttttttataa
     6061 tacttgattc aattgaacag atgattatat ctcttgaaag aagtttagaa gaaaacatct
     6121 atgaaggtat taaaacttta gaatatcaca ttcgacctca aaattatcca attgtaaaca
     6181 agatgcttca attacaaatt aaaacattac aatgggttgt aaataagcaa aaaaagaata
     6241 gtttagaatc tttaaaacaa atagtaaatt ttaaagtcaa aaggttagaa tatgaattga
     6301 aaatagcaag aggagatata gaacatacta gtaaaattgt ctatcaacta gaaatgttgg
     6361 aatgttgcaa aatcatgata aattgggatt tgcaaagaat gacaaaaata acaaaagaag
     6421 atgacgtttc tgcgttttgc taaatttatc aatatgaaaa cacattatga tttaaaatta
     6481 ttttttattc tgatattaga aatcttcatg atatattata ctagccgctc aatcaaccag
     6541 tatactagta atttattatg taatatacta tatattattc taataaacta catagggaat
     6601 ttggcttata gactaaatgg tcaacataac cccgattgtg gaacacaggg agtgaaaagg
     6661 taatatatgc gaggcaacag aactaattac aaacaaaaat ttggtccttg tgaggttata
     6721 tgttgttttc gaatcatcca attggacacg aaatagatta tagaaaaaat gatgagatta
     6781 gacataagaa acgaggatca tatcaaaatg atctgattcc taggaaaagg aaatgtgaac
     6841 aatgttctac cgaaatttgg tatagtattc tataaaaaat cacatacaat attaattgca
     6901 tgaggaaaaa atcctattta tcaatataat gtttagtatt ttaaagaact acaaaattgt
     6961 gtcaaaatcg gttcattttc ctcagagccc tcttaaccaa ctggagctga aaaatggatt
     7021 actacttgta atgaaaatat atatagagtc tttatcatca tcatttgagt ctttatactt
     7081 cattaatttt ttaatattat ttagatagtt atcatatgtg tctttattat cttttgtttt
     7141 atctagacag gttttaaaga aattaccact tttccaatta ctattaatat atttttccaa
     7201 ttcagatgtc ttttctttat gtaaagtaga tataatacaa aaacttcttt tacatccacg
     7261 ttgacatatt aagtatacat atttttgttt gctaataata tttacactaa tgataagata
     7321 gttaagctaa tgagatgata aaaaaatttc aggttatatg tgttgtgtag tattatatat
     7381 ggtaatgtta ttggacattg gaattaacaa atattaaatt agtcttcttc ttgttgagag
     7441 tcttcttctt gttgagcttg ttctgtttgt tctccttctt ctactataat agaaacagta
     7501 gcatcattag agtctatagt accatcattt acaataaatg taaaattgtc ctctcctgag
     7561 taatcctcat caggagtata ggttactgtg ttagtagatg gattcacatt tcctaattgg
     7621 ccattagaag gatcatctac aattgtaaat gataaattat ctccatctac atcatttgca
     7681 tttaatttga tattcaatgg ctgattttgt gatgcagtaa cggaatcatc tcgagcagta
     7741 ggttctgtat taggtgattc ttcttctact tgtggagttt gagcttcatc ttcttcttgt
     7801 tgagcttgtt ctgtttgttg gtcttctata tcttgaattt catcttcagc ttctggatcc
     7861 atctctagaa gttcagatgg ttttccttca atagtatcct ctgttgttat tgcagtttca
     7921 ttgtcagtag gagatgtggc ttcttcttca atagtatcct ctgttgttat tgcagtttca
     7981 ttgtcagtag gagatgtggc ttcttcttca atagtatcct ctgttgttat tgcagtttc
//
LOCUS       DASVEV010000184         4384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1243384, whole genome shotgun sequence.
ACCESSION   DASVEV010000184 DASVEV010000000
VERSION     DASVEV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243384"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            867..1379
                     /locus_tag="VF222_08420"
     CDS             867..1379
                     /locus_tag="VF222_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7142488.1"
                     /translation="MDKDSIYWISKLNLQKHPEGGYFVESYKSEELVNFPKHHGPRHV
                     CTAIYYLLIGEDFSSFHIMKSDELWHFYAGSSLTLYIIEPNGRLNEVRLGHNIDNGDT
                     FQAVVKSGCWFAAAVDDHTSYSLVGCTVSPGFDYQDWKLGDMKKLTELYPQHKSIIEK
                     YTRQSSFSIL"
     gene            complement(1865..2116)
                     /locus_tag="VF222_08425"
     CDS             complement(1865..2116)
                     /locus_tag="VF222_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142489.1"
                     /translation="MIGNLLVIPLQQVFAQVGASEQMMSHMRFTHSQLANNIDQIKQL
                     IQANNTSEALNLLDGMEIKINHMNTMFNDLVWEMSNKGH"
BASE COUNT         1577 a          627 c          586 g         1594 t
ORIGIN      
        1 tattcattga ttgattcata ctttcggctt gtgctaatgc tgatatagtg aatgtcgata
       61 aagtgagcac tgttccaagg aagatggctg ttgctactgt cggtactatt agaaacaatt
      121 tattgtatat catttgttaa ttacttatat tcgattttta tattaaaatt ttttaaaaca
      181 ttaatctatg taacaaatcg gaataatatt ctaaaaaaaa gattactttc tatatttaaa
      241 atgaaattta atgagatact aaattattac ttattgaaaa acattataga tattattttc
      301 ttaccaatat tttataatct tattatattg ctcttgaaga taaatttgtc atgggcatat
      361 cagaaacaat aaatggaatg atattgcaca actttgagct atcaacaaaa aaggatggga
      421 ttctagatag atctgaacaa agttctagtg tacttctcca tggtgattga taggatatat
      481 taattaaaag taaattcttg taaatctcga gacacagaca ctgcacaact ttgtatatat
      541 gtacaaataa attacataat atttttttag caaattatca taccatttct aattaagact
      601 ttaaccttca tttgggttta tcatctcctt caaatctgta tgactctaga tctgtagttc
      661 ttcttctgcg aatatcattc taaaacatat ttttaagata ttaatgagaa agacggattg
      721 ttgtcgtcat gacttttcaa taaggcaata ctcacataag caaaggttaa gacgttggaa
      781 actctagatg aagcatttgt gctgttcttg ttccatccat aacataacat atacataata
      841 aattaatcta tctgaatatc ttactagtgg ataaagacag tatatattgg atcagcaaat
      901 tgaatcttca aaaacatcca gagggaggat acttcgttga atcctacaaa tctgaagaat
      961 tagtaaactt tcctaaacat catgggccta ggcatgtttg tactgctatc tattatttac
     1021 ttataggaga ggatttttca tcttttcaca taatgaaatc agatgagctg tggcattttt
     1081 atgctggcag cagcttgact ttatacataa tagaaccaaa tggaagatta aatgaagtga
     1141 gattaggaca caatattgat aatggagata cctttcaagc agttgtaaag tcaggatgtt
     1201 ggtttgcagc agcagttgat gaccacacat cttattcttt agttggctgc actgtttcgc
     1261 caggattcga ttatcaagat tggaagttag gcgacatgaa aaaactcact gaattatatc
     1321 ctcaacataa atcaattata gaaaaataca caagacaatc ttctttttct attctttagg
     1381 taaacaaatc atgatagtat tacagtgtat gttttaattt gtaaaaaaat ttatctcaag
     1441 tactactttc tatacaagaa gagcagaaca gcccttgata aatatactta tgtggctatc
     1501 taggttatta gcatgaataa tattactaaa taagataata atataataat taagttacta
     1561 gcaaaagaac gtttagttgc tatctcagcc gacatattaa actgatttta aactcgattt
     1621 atagaaaaat aaactaatgc tgaagaggct actattgaga atatcatgtg tgcttacaaa
     1681 gattgggata ttgagttaga gtcgcataga tcataaaaaa actagaatcg ttatttacta
     1741 tataagatat attgaaatag ctccaaatat atttcgcagc atctgtttta gaaattaaaa
     1801 tataaatttc atttatcgta aataaaaatt tggatataaa aatttaaggt aataatttag
     1861 agtattaatg acctttattg gacatttccc atacaagatc gttaaacata gtattcatat
     1921 ggttaatctt gatttccata ccgtcaagta gattaagggc ctctgaggta ttattggctt
     1981 ggatcaattg tttaatctga tcgatattat tagctagttg ggaatgagtg aatctcatat
     2041 gactcatcat ttgttctgaa gcccctactt gggcaaatac ctgctgtagt ggaataacca
     2101 aaagatttcc aatcactaac aaaagtacca gaatggtctt catattatta ttaatattat
     2161 acaattaaat tccttttaaa catttaggat attacctact atattaatgt aaaattttaa
     2221 acattgtttg aattaaagac tcattattta tttatctcct caatgataat actagattca
     2281 ctaaattaac tttagaaatt gcaaagattc tagaatctga atttctctct gaatagggta
     2341 aatcatttat ctcaccaaac ttttttctct cttatcgatt tatgagtcta acagaaataa
     2401 atttccctct tctgtcacaa gtttttattt atttttcttt atcgtcttgg aaacctcaag
     2461 tattattaat tgtgttaatt aataaattgt aaatagttta gtattattgt attatgtaaa
     2521 attttctcaa tatccattta ttatatctgt gcacttatca tctcattatc ttaaaaatca
     2581 atattattat cagatactat taacaagaca ctcggcgatt tcaggccatt atgttttttt
     2641 ggtagcaata ctctatcaat cacatcacaa cctatttttt attcaccatc attttcacca
     2701 tcaacatcat catttccatt gaaattgtta accaggaata ctttaccact aggattaatt
     2761 ctagggtttt gttaatattt gtgagtgcca gtgtaaactt atcttttaaa agtttagtac
     2821 tgttttaaat tgtggaatag caatttgact tttttaatat gaataaagct ccagttacta
     2881 aatagataaa cttgctccag ttactaaata gataaacttg ctatgattta tagatagtta
     2941 gttacagata gctaaataaa aatagaacct attatgttta ttaatcttaa gatatctcac
     3001 tgattgccaa aaatttattt atgagaaatg tacagtgtat tgtatcactg aataactata
     3061 ttaattttct ataataatgt ccatttttga gtaggtatat aacgccattc aatgtctttg
     3121 tgttcattca ctattataga gaatagcacc ttctaccata atagagttat tcagtgatca
     3181 tgaatgggaa atatcctacc cataggcata taattgaaag gataatttag cagtaataaa
     3241 gcagagcttt agttcattaa cctattttac tgttcttctt tactactact gttatcatta
     3301 tttctttctt cccattccaa tttaataacc taataatagt aataattatt gtataaattt
     3361 tcttttgtat agttttactt attctttcga gctaggttgt gttactttat cttgtcctct
     3421 taaaacaata aggaaaaaat ataggtagaa ttgaaaatat tgatacgtta acgaataact
     3481 taaactgaga aaactagacc cggtttagag taatttctat agcattacct aaataatagt
     3541 ctaattttaa aatgataatg agtaaaatat ttaatattca ttaaatacta tgtttttaaa
     3601 agaaattttc ttctattatt tatgataatt tataattctc tataaataaa tttgttgaat
     3661 tttctaattt aaatgaaagt aaaaataatg caatagctct tttaatatgc cttttctttg
     3721 tctccagtaa aataatagca gcttgtactt accattagga taatccttct aaacttccta
     3781 gtaaagcaag gtcaaggaca tttttaatat tagcaatcat cctattacca actacttatg
     3841 ataaatttga aagcgatgaa aaaatatttg aactgcaaaa ggttgataaa catctgaaaa
     3901 aatgaagtca gacagataag tgcctattct atatagatga caagattgta gaagttcaca
     3961 aaaaactaca aaaaaaagat aaacaagaca aaactcagtt tcaccttcaa ctgaatcgta
     4021 tataaaaata taacgaaaaa acacaaataa gtctagtcaa tattcattct gaatttaaag
     4081 gtcaagcatt atcattaaaa gatcttaata tctattcatc catttaataa actcaattat
     4141 cttctgacct gtcttttgag gaaaccttaa gagggtttac tttccattct tgtataaaat
     4201 ctaaatgtcc cacaggtagt acaattccta caaattttcc taaaccttta tcaaaaaatt
     4261 aaatttggca aatgggtaaa aggtttttgt tatcttatta taatctatgt tttatggaaa
     4321 accaatttac gaaatattta attaaatgta tttaactgat atttaatcat ggagctgcac
     4381 cacc
//
LOCUS       DASVEV010000185         8849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1049316, whole genome shotgun sequence.
ACCESSION   DASVEV010000185 DASVEV010000000
VERSION     DASVEV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049316"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VF222_08430"
     CDS             <1..271
                     /locus_tag="VF222_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142490.1"
                     /translation="QVIKLKNNRSITLNRLPNKTTGTEYNIYVEKILPGKAYVLVNER
                     WYALLNYYDYEGPRNLLKKGSEFKAIAELYTNGSDFCIRIKEIIL"
     gene            complement(315..1082)
                     /locus_tag="VF222_08435"
     CDS             complement(315..1082)
                     /locus_tag="VF222_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142491.1"
                     /translation="MPIPTPNKLLATYVIDITTGSGITYKFQHFYPLNIAIPVDTTVA
                     WFNDDPEQIHTVTSEKPGSSDSGKLFNSGILPYSSSFQYTFNQPGKFLYHCEIHPWMI
                     GSVYASDSNKQGKNFKLTTGTKMALEDNSPYDWSFNTTEIDRILFNIEPLSVNTDKKT
                     PITYNIDIYNNELKKEILSKSFFARGNDFQFELLSSDLDQPLVYGPDLSDPIIGTYHI
                     RDNFPDGTYILKIEITGIGSNLTQTNISDEFKGIISS"
     gene            1305..1958
                     /locus_tag="VF222_08440"
     CDS             1305..1958
                     /locus_tag="VF222_08440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7142492.1"
                     /translation="MQFANNMIIDLSQSINSDIKLYPGSPNVYFLKWSKYSIDGYDAE
                     AIFLSTHTGTHMDAPSHFIEGSEPIDDIDVNRFVMENVHLLKILKSSNQLITVEDIIN
                     SDVDIKENDSIVFSTGWEHNYNSDNYISSNPGLSPEAATYLSNKKINAVAIDSPSIDS
                     GIESEFPVHQILLKNNIIIIENICNLAQIDKKNFRLIAIPLKLRGASGSPVRALAII"
     gene            2089..2727
                     /locus_tag="VF222_08445"
     CDS             2089..2727
                     /locus_tag="VF222_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7142493.1"
                     /translation="MPTNWLTWARGNDKLILGALEKQGFILVEATSLLTELLTDYEKS
                     LSENKDKIKDLEVKGDSITHELFTTLSQTFVTPLDREDLSGLTSTIDQVLDYVDGTAD
                     RFVLFKIKKPMPYMLDLAKILQSCSQEIHEVLIKLQSSKKNGKFIEHCNKIKKHEREA
                     DHLYRKAIAELFETNDAVQILKNYEIYQNLESSIDRCMDVADIVEDIVLKYG"
     gene            2753..3757
                     /locus_tag="VF222_08450"
     CDS             2753..3757
                     /locus_tag="VF222_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7142494.1"
                     /translation="MEPISLIITGIIAALFFDFVNGFHDAANAIATVVGTRVLKPIQA
                     VGMAAIANFIGPFVFGTAIAATVGKGIIQPNFSTAEVILAGLVGAIVWDLITWYFGLP
                     SSSSHALIGGLIGSALVSGGFEAIVFGGVEKVLTFLVVSPVLGFVIALGFGILLIYFL
                     KKYHPGKINRVFGKLQIASSAFFSLTHGANDGQKTMGVITALLMTGGFLDAEHFEVPT
                     WVILSASIAIAFGTFLGGWRIVKTMAFRITELKPYQGFCAETGGGVILTSMAWLGIPV
                     STTHAIAGAIMGVGSTRRLSAVRWGIGKKIIYAWIITIPASAGIGALVLFLFNLLGLE
                     "
     gene            complement(4055..4246)
                     /locus_tag="VF222_08455"
     CDS             complement(4055..4246)
                     /locus_tag="VF222_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142495.1"
                     /translation="MKCNQCEDGFISNHPMEAPICGICLQKIKSIDLTVDTEKKCIIC
                     GRNGAVYSSNKCNDCYFKK"
     gene            complement(4611..5141)
                     /locus_tag="VF222_08460"
     CDS             complement(4611..5141)
                     /locus_tag="VF222_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142496.1"
                     /translation="MKINVLIPLFVLIIIIVTNSTSFVYGQNSNNTNIFDNILKNLKN
                     KIIDLTKGVEYTIDIKANQIFPNDTIKNSILNKNQPMEYLIPILEYKLLGFDIFATDI
                     KVRAESKKIYEDNKNKLRIEFPIMEAKNVNVNNKIFDLNYENIDLSSTYTIYDSELDK
                     FTIHIPVYVATRYLPK"
     gene            5277..6764
                     /locus_tag="VF222_08465"
     CDS             5277..6764
                     /locus_tag="VF222_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142497.1"
                     /translation="MNEGVPSNPKDKNTKWNKIPQIAWFTLFILGSVDLISTAGETMI
                     IPAIPDIINEFNITYSDSSWILSSYLIAGAVMTPIASKLSIIFGKKEILLTLMILYVI
                     GTLLAGFAMNFSFLIIARVIQGLGLAMFPLAFSIIQTVFPKESLAIGQGIIIALFSSG
                     SVIGLVVGGYVIDNFGWQIIFFVFVPISILTIILINKKINIPNEKISINENTESINKL
                     TNKKLSKLNLIDFKGTITLALLISSFLIVLSNLEEFDPQQFQLTIIFTIITPISLILF
                     ILFSKRNKNPVISLGIFKQKILLFTNIILMISGLTTFMIYQMLPILIRNPFPVGFEGD
                     AIKVVEIQLPFMIVLFLVSVFSGFLVSKLGNIKPTLLGSMISLLGFFSIFFFHFSEFA
                     IIVNLVIIGIGISFLQVGGFNIITASTPKKLIEISVGVTSLLFIIGMSIGPVISSLFL
                     QNFKTTIYENNNFLPSSEAYDLIFLTAASISLLPTILIICIKKLK"
     gene            complement(6845..7825)
                     /locus_tag="VF222_08470"
     CDS             complement(6845..7825)
                     /locus_tag="VF222_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142498.1"
                     /translation="MEISEKETMKTSVLYINSFIGILSSFLFFLSIASISNVYAVISD
                     FNFAAVGDWGCNSNTDATVKGMANTKTTNKLGAPELVLGLGDYSYQPTANCWLSKVAS
                     IDDNMKITIGNHENSAKTGFNQYKKSFGLTSNAYYSFDFQNIHFIIMNTQTPYTAGSS
                     QYNFIKSDLAKLANKANNDWIIVVMHEPFYTSPTSCKINSCQGIGNLRDIYHPLFDTY
                     EVDLVLGAHLHDYQRSFPLEYNSASSAKPIMTSKILSKYNDPKGEIYLIVGTGGINFH
                     KLIGKNSYIVNQQSSFFGHLNVDISNKGQVLTAKFIQNNGKVFDSFSITK"
     gene            complement(7835..8209)
                     /locus_tag="VF222_08475"
     CDS             complement(7835..8209)
                     /locus_tag="VF222_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142499.1"
                     /translation="MLPIIMSMTIIIPLFTLPAYAQKNVTISPNCGTEEGFEVTLETD
                     GFGQNSNVHWDLVDTKNNTIALSGYFATNSSGGFEEPTFVDDINSGDYKIKVFDDADT
                     NDKLDTNKTELSLNFSIPCKNQ"
BASE COUNT         3104 a         1161 c         1295 g         3289 t
ORIGIN      
        1 acaagtaatc aaattaaaaa ataatagatc aataacttta aatcgattac caaataaaac
       61 aacaggaaca gaatataata tctatgttga aaaaatttta cctggcaaag catatgttct
      121 ggtaaatgaa agatggtatg cattactaaa ctattatgat tatgagggac ctaggaacct
      181 tttaaagaag ggatcagaat ttaaagctat tgcagaatta tatactaatg gaagtgactt
      241 ttgcattaga ataaaggaaa taatacttta attaaagtca tccgaccagt tttaggacaa
      301 ttcatcagtt aatcttacga agagattata cctttaaatt catcagatat atttgtttgt
      361 gttaaattag atcctatacc tgttatttct atttttagaa tataagttcc atcaggaaag
      421 ttatctctta tatgataagt acctataata gggtcactaa ggtcgggacc ataaactaga
      481 ggttgatcta aatcactgga taaaagttcg aattgaaaat cgttacctct ggcaaaaaaa
      541 gattttgaaa gaatttcttt tttaagttca ttattgtaaa tatctatatt gtatgttata
      601 ggtgtctttt tatcagtatt tactgataag ggttctatat taaacaaaat tctatcaatt
      661 tcggtagtgt taaaactcca atcatatgga gaattatctt caagtgccat ttttgtacca
      721 gtggtaagct taaaattttt tccttgtttg ttagaatcac ttgcatagac tgatcctatc
      781 atccatggat gaatttcaca gtgatacaaa aatttacctg gctgattaaa cgtatattga
      841 aaagaggatg aataaggaag aattccagaa ttaaatagtt ttccagaatc agatgagcca
      901 ggcttctcac ttgtaacagt atgaatttgt tctggatcat cattaaacca tgcaacagtt
      961 gtatctacgg gtattgctat atttaatgga taaaagtgtt ggaatttata agttattcca
     1021 gaacctgtag taatatctat aacatatgta gctaatagtt tattaggagt tgggattggc
     1081 atctttccta attctcctac aatacttgga atattaaaat ataaaataat actactaaaa
     1141 attaataatg ataatgttaa aaattttaac attatattaa tatgcataaa ctactttata
     1201 tcattttagt tttatatgca atagttttat ttaaaatact tattggatat cgacttctta
     1261 gttattaaat tatcttgtat tcttttgaaa taattaaaag ggagatgcaa tttgcaaata
     1321 atatgattat agatttaagt caatcgataa actcggatat taaactttat cctggatctc
     1381 caaatgtcta ttttttaaag tggtctaaat atagtattga cggctatgat gcagaagcaa
     1441 tttttttaag cacacatact ggaacccata tggatgctcc ttctcatttt attgaaggat
     1501 ctgagcccat agatgatata gatgtaaata gatttgtcat ggaaaatgtt catttgttaa
     1561 aaattttgaa gtcatctaat caacttatca cagtagaaga tataatcaac agtgatgtag
     1621 atattaaaga aaacgattct attgtttttt ctactggatg ggaacataat tataattccg
     1681 ataattatat aagctcaaac cctggcctct ctccagaggc agctacctac ctatctaata
     1741 aaaaaattaa tgctgtagca attgatagtc ctagtatcga ttcaggaata gaatcagaat
     1801 ttcctgtaca tcaaatactt ttaaaaaata atataataat aatagaaaat atatgtaact
     1861 tagctcaaat agataaaaaa aattttaggt taatagcaat tcctctaaaa ttgcgtggag
     1921 catcgggatc tcctgttaga gctctagcta taatctaata tctataataa ataataatta
     1981 caaaagttat aaacgcatta ttattattaa caatcagaat catcagtaaa aaaatatcta
     2041 atgctttata aaatattaaa ttttattgtg ttaaggtgat atggagattt gcctacaaat
     2101 tggcttactt gggctcgtgg taatgataaa ttaatccttg gtgctttaga aaaacaagga
     2161 tttattttag ttgaagcaac atccttactg acagaacttt taactgacta tgaaaaatct
     2221 ctttctgaaa ataaagataa gataaaagat cttgaagtta aaggagatag tattactcat
     2281 gagctattta caactttatc gcagacattt gttactccat tggatcgaga ggatctatca
     2341 ggattgacta gtacaataga tcaagtttta gattatgtag atggtactgc ggataggttt
     2401 gtactattta aaattaaaaa acctatgccc tatatgttag atcttgctaa gattttacaa
     2461 tcatgttccc aagaaataca tgaagtgtta ataaaattac agtcatcaaa aaaaaatgga
     2521 aagttcatag aacactgtaa taaaattaaa aaacatgaac gtgaagcgga tcatctttat
     2581 agaaaagcta tagcggaatt atttgaaact aatgatgctg tgcaaatact taagaattat
     2641 gaaatttatc aaaatctgga atcttccata gatagatgta tggatgtggc agatatagta
     2701 gaagatattg ttctcaaata tggataattg ttcattgtag gatgatgtta aagtggaacc
     2761 tatatcgtta attataacag gaataattgc agctcttttt tttgatttcg taaatggttt
     2821 tcatgatgca gctaatgcaa tagcaactgt tgtaggaact agagttttaa agccaattca
     2881 agcagtaggt atggcagcta tagctaattt tattggtccg tttgtttttg gtactgctat
     2941 cgcagcaact gtaggtaagg gaattataca accaaatttt tcaactgctg aagttatttt
     3001 agctggacta gtgggagcaa tcgtatggga tttgattact tggtactttg gtttaccatc
     3061 atctagtagt catgcgctta ttggtggatt aattggctct gcattagttt ctggtggttt
     3121 tgaagcaata gtttttggag gtgttgaaaa agtattaaca tttcttgtag tttctcctgt
     3181 tcttggattt gttattgctt tgggatttgg aatattgtta atatattttc tgaaaaaata
     3241 ccaccctgga aaaataaatc gagtctttgg taaattacag atagcatcct ctgcattttt
     3301 ctctctgaca catggagcaa atgatggaca aaaaactatg ggagtaatca ctgctttact
     3361 aatgactggc ggttttttgg atgccgaaca ttttgaagtt ccaacttggg taattctttc
     3421 agcttctata gcgatagctt ttggaacatt tttgggggga tggcgaatag taaaaacaat
     3481 ggcttttcgt attaccgaat tgaaaccata tcaaggcttt tgcgcagaaa ctggaggtgg
     3541 ggtaatatta acttccatgg catggttagg aatcccggtt agtacaactc atgccatagc
     3601 aggagctata atgggtgtag gttccacacg aagactttca gcggtacgat ggggtattgg
     3661 aaaaaaaata atttatgctt ggattattac aattcctgcc agtgcaggga tcggtgcgtt
     3721 agttttgttt ttatttaatt tattaggact tgaatgagcc aatgatccta ctagtgaact
     3781 tataacagaa tcttgatatt gtggtttatt tatttataaa atagatgaag ttgattttaa
     3841 ttaataggta aatgtgtaaa ttactatata tattctacaa aaacatatta tatacgaaat
     3901 ataatgaatg gatataacgg taacaaaaag tagcgctaat actataattt atatattgaa
     3961 aaaccagtaa aattgtgaag tattataaat tagagatatt gattaagtta catcaataat
     4021 ttataagaac ctttaataag atataataaa taacttattt tttaaaataa cagtcattac
     4081 atttattgga agaataaaca gctccatttc ttccacatat aatacatttc ttttccgtat
     4141 cgactgttaa gtcgatcgat ttaattttct gtaaacaaat gccacaaatt ggtgcttcca
     4201 ttggatggtt agatataaat ccgtcctcgc actggttaca tttcaaacta tagatttttc
     4261 cttttggctt ccatgtcttt cttctgatcc aatttaaaat caatttttta cttattttac
     4321 atagaattta aagataatta aatttttgca tctaaaataa ttcaactaat atattaaaag
     4381 cttataatat ttataaaaaa aagtaatatt agaaaagaaa tataattaaa acaaattaaa
     4441 atataaattt atgctacttt ataataaaat tcaaaacttt tgattaatat tgatatacac
     4501 tcctttagat ttaaaatcta ataatattaa ttatgtcata tgagtaaaat ttaaagaata
     4561 ttcttgcgat aattttcgaa cttgcttaat agatatttta ctttctatat ttattttggt
     4621 aaatacctag tcgctacata tactggaata tgaatagtaa atttatctaa ttctgaatca
     4681 taaattgtat aagtagagct taaatcaata ttctcataat ttagatcaaa aattttatta
     4741 tttacattaa cattctttgc ctccataata ggaaattcta ttcttaactt atttttatta
     4801 tcttcataga ttttctttga ttcagctctt acttttatat ctgtagcaaa tatatcaaaa
     4861 cctaataact tatattctaa aataggaatt aaatattcca ttggttggtt tttatttaat
     4921 atagaattct tgatagtatc atttggaaag atttgattgg ccttaatatc tatggtatat
     4981 tccacacctt tggttaaatc gataattttg ttttttaaat tcttgagtat attgtcaaaa
     5041 atgttagtat tattagagtt ttgaccatat acaaacgatg tactatttgt aactattatt
     5101 attattaaaa caaataaagg aattaaaaca ttaattttca tatatgataa atattaattt
     5161 aagattttat agtctctata tattctttga tttggtttaa ctaaaacaaa atcttgtgct
     5221 tgttgcatct attcaatatc tttttattta tataagaaat aattccataa aaataagtga
     5281 atgagggcgt tccatctaat ccaaaagata aaaacactaa gtggaataaa attccacaaa
     5341 tagcatggtt tacgcttttt attcttggtt ctgtggattt gatttctaca gcgggtgaaa
     5401 caatgataat acctgcaatt cctgatatta tcaatgaatt caatattaca tattctgatt
     5461 catcttggat attgagctca tatttaatcg caggagcagt aatgacacct attgcatcaa
     5521 aattgtctat catttttgga aaaaaagaaa ttcttttgac tttaatgata ttatatgtaa
     5581 ttgggacact attggcagga ttcgctatga atttttcctt tttgattatt gcacgagtaa
     5641 ttcaaggctt aggacttgca atgttcccat tggccttcag tattatacaa acggtttttc
     5701 caaaagaatc gcttgctata ggtcaaggaa taatcattgc tcttttttcc tcaggatctg
     5761 tcattggtct agtagttgga ggatatgtaa tagacaattt tggatggcag attatttttt
     5821 ttgtatttgt tcctatttct attttaacta taattcttat taataaaaaa ataaatattc
     5881 caaatgaaaa aatttcaatc aatgaaaata ctgaaagtat taataaatta actaataaaa
     5941 aattgagtaa attaaatcta attgatttta aaggaaccat aactttagca cttttgatat
     6001 catcgttttt aatagtatta tcaaatttag aagaatttga tcctcagcaa tttcaactaa
     6061 caattatttt caccataatt acaccaatta gtcttatact gttcatatta ttttcaaaaa
     6121 gaaataaaaa tccggtcatt tctcttggaa tctttaaaca gaaaattctt ctttttacaa
     6181 atatcatttt aatgatatca ggtttaacaa cttttatgat ttatcaaatg ttaccaatat
     6241 taatacgtaa tccttttcca gttggttttg aaggtgatgc aattaaagta gttgagattc
     6301 aacttccatt tatgattgta ctatttttag tctctgtttt ttcgggattt ttagtttcca
     6361 aattaggaaa cattaaacca actttattag gaagtatgat aagtttatta ggattcttta
     6421 gtatattctt ctttcatttt tcagaatttg ccattatagt taatttagtt attattggaa
     6481 taggaatatc ttttttgcaa gtaggtggat ttaatataat aacagcatca accccaaaga
     6541 aattgattga aatctcagtt ggagtgactt ctttattgtt tattattgga atgtcaatag
     6601 ggcctgttat ctcgtcttta ttcttacaga attttaaaac tacgatatat gaaaataata
     6661 attttttgcc ttcttccgaa gcctatgatt taattttctt aactgcagca agtatttcat
     6721 tattgcccac tattttaatt atatgtatta aaaaactaaa ataatttagt caattattat
     6781 tctgaattgt tagactaggt ggatttagat tagaaattac aaaaaatttt atttgttgta
     6841 tttgtcattt tgttattgaa aatgaatcaa atacttttcc attattttga ataaacttag
     6901 cagtcaggac ttgtcccttg ttagatatat ccacatttaa gtgaccaaaa aaagaacttt
     6961 gttgatttac tatgtatgag tttttaccta tcaacttatg gaagttaatt ccaccagtac
     7021 ctacaattaa atatatttct cctttaggat cattatactt tgacagaatt ttactagtca
     7081 taataggttt tgcactactt gcagagttat attctaacgg aaatgatctc tgataatcat
     7141 gtagatgtgc tcccagaact aaatctactt catatgtgtc aaaaagtgga tgataaatat
     7201 ctcgcaaatt acctatacct tgacacgagt taattttgca acttgtaggt gatgtataaa
     7261 aaggttcatg cattactaca attatccaat cattattagc tttgttggct agttttgcta
     7321 aatcactctt tataaaatta tattgtgaag aaccagctgt atagggagtt tgagtattca
     7381 ttattataaa atgaatgttc tgaaaatcaa atgaataata tgcattcgat gttaggccaa
     7441 atgacttttt atattggtta aatccagttt tggcagaatt ttcatgattc cctatagtaa
     7501 ttttcatatt atcatctatg gatgcaactt tactgagcca acaatttgct gtaggttgat
     7561 aggaataatc tcccaaacct aacacaagtt caggtgctcc aagcttattt gtagtttttg
     7621 tattggccat tcctttaaca gttgcatcgg tattagaatt acatccccaa tctcctacag
     7681 ctgcaaagtt aaaatcagat attacagcat aaacgttaga aatgcttgct attgataaaa
     7741 aaaagagaaa tgatgataat ataccaataa atgaattgat atataaaacg ctggttttca
     7801 tagtttcttt ttcactaatc tccattttat attattattg gtttttgcat ggtatagaaa
     7861 agtttagaga aagttctgtc ttattcgtat ctaatttatc atttgtatcc gcatcatcaa
     7921 agacctttat tttgtaatct ccactgttaa tatcatcaac aaacgtaggt tcttcaaaac
     7981 caccactact attggttgca aaataacccg agagtgcaat tgtgttattt tttgtatcaa
     8041 ccaagtccca atgtacatta ctattttgtc caaatccatc agtttcaaga gttacttcga
     8101 aaccttcctc tgtaccgcag ttaggactta tagttacatt tttctgtgcg tatgctggta
     8161 aagtaaataa tggtatgata atagtcatac tcattataat cggtaacaaa atataatacc
     8221 tactcattaa ataagttcgt tataataaat atataagcaa ttcttttttt gttgtaatca
     8281 ataaatatat ctcactatca ttatagagtt aaaggtttgt gtatttgtaa ttttacagtt
     8341 tattcaattg aatttgaaaa taatatgtta taattgatat gttataattt agattctctg
     8401 tatagttctt tttaattgtt ataaatttat aacttctata tatgtgatat tattaaatgg
     8461 gcattttatt ttatattatt atactataga tgtcaatttt gaataataat ttttaatgaa
     8521 atatttatat ataatatata aaactatcta acaacttaac aggatgattt ttgtgaatat
     8581 attaaaaaat aacatttatt ataaaaagcg attaataaat taaaattttt aacgcttgaa
     8641 tatttttata taggttaaat tataatataa gcgattataa tagtgaaaca aaattgattc
     8701 atgatgcgca atctattgca aaaaggtttt tttctaaccg aaatgctgct agctatgatc
     8761 gaatagtaaa atatactaca tttggaagag attcattgtg gaaacaaaat attgctaatt
     8821 atataactcc agaacataaa aatatacta
//
LOCUS       DASVEV010000186         4929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1049333, whole genome shotgun sequence.
ACCESSION   DASVEV010000186 DASVEV010000000
VERSION     DASVEV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049333"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VF222_08480"
     CDS             complement(<1..487)
                     /locus_tag="VF222_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142500.1"
                     /translation="MLKISSFLFGIIITSIILFSANPPSLSIYAQDNQYYYNTTNETN
                     SSLLNELENIKNILESKVTKLATALQIASSLPQILHPPDINLIDPKVKGIPEDADLEK
                     RKIGKILLEQFNEFFSVVFLLNNGDVYFDEPFERQLNRTATNLSFRDYYQAVEQTKKT
                     YL"
     gene            858..2810
                     /gene="acs"
                     /locus_tag="VF222_08485"
     CDS             858..2810
                     /gene="acs"
                     /locus_tag="VF222_08485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7142501.1"
                     /translation="MPIDKDNSSSDINFTSSHLKITLDKSTFQIKRAQSLQNTEKFWT
                     EQAKNLVWNRQWDKTLEWNPPFAKWFVGGLVNASINCLDKHINSEIKNKAAIIWEGEN
                     GENITLTYYQLYQKVNQFSNALKNLGIRKGDCVTIYLPMVPELPIAILACSRIGAIHN
                     VIFSGFSSTSLVDRINDAKSKIVITADGGFRRGKIIELKKIVDEAIINTPSIQNVVVL
                     RRTGNHIEMKDKDIWWHEIIQNQSYYCEPELIESTHPLFILYTSGTTGKPKGVVHSTG
                     GYLTHIYSTAKWVFNYNQQDIFFCTADIGWVTGHSYIVYAPLMHGVTEIMYEGTADYP
                     HPDRYWSIIEKYGVTILYTTPTALRMYMKYGNEIPNSFNLSTLRLLGTAGEPINPEVW
                     MWYFTIIGKEKCPIVDTWWQTETGGIMISYCPGIDIMPMKPGSAIFPVLGVDLDVVNE
                     EGNILPQNQKGYLVIRKPWPGMLMTLWNDDDKYKNNYWKKNYYFTGDYATIDDEFYFW
                     LLGRSDDIIKISGHRLGTIELESTFISHESVAEAAVTSKPDKIKGESIIAFLVLKKGF
                     VESDQLRKELIEHVRNNIGAIATPNEIYFVNKLPKTRSGKIMRRLLKSIVSGLTIGDT
                     TTLEDGTSVKEIQKIYNDLNNIIKNK"
     gene            3360..4022
                     /locus_tag="VF222_08490"
     CDS             3360..4022
                     /locus_tag="VF222_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7142502.1"
                     /translation="MKLEVLFIPVSDVDRAKRFYEKLGFRLDIDYVANEDFRVIQFTP
                     PSSEASLIFGKGITSAQPGSIDPSVLAVYDIDAARDDLITRGVDVSEVFHYAGGPFNN
                     VMENPRVSGRDPQGRSYFSFASFKDPDGNGWLLQEIQTRLPNREWNLTLPQAMDTATL
                     AGLLSETAEHHGNFEKTHAEHQWWDWYAPYLSARQSGSNPEKAAAAADRYMEEVLHVL
                     PK"
BASE COUNT         1739 a          734 c          842 g         1614 t
ORIGIN      
        1 tcaagtaagt ttttttagtc tgttctactg cttgataata atctctaaat gaaagatttg
       61 ttgcggttcg atttaattga cgttcaaatg gttcgtcaaa atatacatct ccattattta
      121 ataaaaatac aactgagaaa aattcattaa attgctccaa cagtatttta cctatttttc
      181 tcttttctaa atctgcatct tctggtatac ccttaacttt tggatctatc aaatttatat
      241 caggagggtg caaaatttgt ggtagactac tggcaatttg taaagccgta gcaagttttg
      301 ttaccttact ttcaagtata ttttttatat tttcgagttc atttaaaagt gaagaattag
      361 tttcgttggt ggtattataa tagtattgat tatcttgagc ataaatggag agagaaggtg
      421 ggtttgcaga aaataagata atactagtaa taataatacc aaataaaaaa cttgaaatct
      481 ttaacattat ctattcttga gaattttaag aataattcaa tataattcta tctatgacag
      541 aataattttg ttgttgatat actatcttat tagaaaaagg atattgttga aaacatttag
      601 tatagtagta agggttctga gggcttcaaa ttctcattat aacagggtta ggtaacataa
      661 tattaaaagt tgtctctatg tataatgata caatagcaaa taataataat tattttgagt
      721 tagaggaggt gagaatatta tcttaaatta acagagcccc ccttttaata gatataaata
      781 ttttagttaa acaacattaa catatagtta tgcatattac aataatattt aattaataag
      841 aactgtcatt gtaaatgatg ccgattgata aagacaattc atcatctgac ataaatttta
      901 caagctcaca tttgaaaatt acattagata aaagtacttt tcaaattaaa agagcacaat
      961 cgttacaaaa tactgaaaaa ttttggacag aacaagcgaa aaatttagta tggaatagac
     1021 aatgggataa gactttggag tggaatcctc catttgccaa atggtttgtt ggtggattag
     1081 taaatgcatc aataaattgc ttagacaaac atattaattc tgaaataaaa aataaggctg
     1141 ctataatatg ggaaggagaa aatggagaaa atataactct aacttattat caactctatc
     1201 aaaaagttaa tcaattttca aatgctctaa aaaatcttgg tataagaaaa ggagattgtg
     1261 taacaatcta tttaccaatg gtcccagaac ttccaattgc aattttagct tgttcaagaa
     1321 ttggtgctat tcataatgta attttttctg gttttagttc tacatcattg gtagatcgga
     1381 taaatgatgc aaaatctaag atcgtaatta ctgctgatgg aggatttaga agaggaaaga
     1441 taattgaatt aaaaaaaatt gttgatgagg ctattattaa tactccttca atacaaaatg
     1501 ttgtagttct acgacgaacg ggaaatcata ttgagatgaa agataaagat atttggtggc
     1561 atgaaataat ccaaaatcaa tcatattatt gtgaacctga attgatagaa agtacgcatc
     1621 ctctttttat tctttataca tctggaacaa ccggtaagcc caaaggagtt gttcatagta
     1681 ctggaggtta tttgacacat atttattcta cagcaaaatg ggttttcaat tataatcaac
     1741 aagatatttt tttctgtact gcggatatag gatgggtaac aggtcatagc tatatagttt
     1801 atgcaccttt aatgcacgga gtaactgaga taatgtatga aggaacagct gactatccac
     1861 atccagatag atattggtca attattgaaa aatatggagt aactattttg tatactacac
     1921 ctactgctct tagaatgtat atgaaatatg gcaacgaaat accaaattca tttaatctat
     1981 caactcttcg attactagga acagcaggag aaccaattaa cccagaagta tggatgtggt
     2041 attttacaat aattggaaag gaaaaatgtc ctatagtaga tacatggtgg caaacagaaa
     2101 caggcggaat aatgattagc tattgtccag gaatagatat tatgccaatg aagccagggt
     2161 cagcaatatt tcctgtacta ggcgtagatc tagacgttgt aaatgaagaa ggaaatattt
     2221 taccacaaaa tcaaaaaggt tatctagtta ttagaaagcc atggccaggt atgttaatga
     2281 ctttatggaa tgatgatgat aagtataaaa ataattactg gaaaaagaat tactatttta
     2341 ccggagatta tgctactata gatgatgagt tttatttctg gcttttaggt cgatcagatg
     2401 atattattaa aatttctgga catcgattag gtacaattga attagaaagt acctttatat
     2461 cacatgaatc tgtagccgag gcagcagtta ctagtaaacc tgataagata aaaggtgaat
     2521 ccattatagc atttttagta ttaaagaaag gttttgttga atcagatcaa ttacgtaaag
     2581 aattaattga gcatgtcaga aacaatatag gagctattgc tactccgaat gagatatatt
     2641 ttgtaaacaa gctaccaaaa acaagaagtg gcaaaataat gagaagatta ttaaaatcta
     2701 tagtcagtgg tttaactata ggagatacta cgaccctaga agatggcaca tccgttaaag
     2761 aaattcagaa aatctataat gacttaaata atataataaa aaataagtag aaatttataa
     2821 ttattattat tattattttg atttgattat ttattaataa aggtaaaatt caaatcatag
     2881 agtcattata ttacttgttg aattggttcg caagatggct gtcctaattc cggctgccat
     2941 tacatctttt tttattgaga tagcagaata atttttaaaa taataaaaga tgagataatg
     3001 agttcatgtg ttcataaaag attacaacaa aaaatatttt tcataaaata ttaaaacact
     3061 tattatctat ttaaaaaata ctaaagttaa gagtaggatt taatccttct ataaagtggg
     3121 acaagctact tttttttatt taattgaata acctatcata ttttatatag aataaagtta
     3181 gtaaatagag tatatttcta gaactgacaa attagcattg aaacttaggg gaagatccaa
     3241 tagattgtga tagatcaaaa aaaatttctt tcttataggc acttaacttc ctattaataa
     3301 ttatatttcc aatagcgtac taattaaata aaagaaaagg agtataattg gtcatggata
     3361 tgaaactaga agttttgttc atacctgtct ctgacgtcga tcgcgccaag cgcttctacg
     3421 agaaactggg atttcgcttg gatatcgact atgttgccaa cgaggacttt cgagtgatac
     3481 agttcactcc tccaagttcg gaggcgtcgc tcatcttcgg caaggggatc acttccgccc
     3541 agccgggctc tattgaccct tcagtcctgg cagtgtacga catcgatgcc gcccgcgatg
     3601 acttgatcac ccgaggcgtc gacgtaagcg aggttttcca ctacgccggt ggtcccttca
     3661 acaatgtcat ggagaaccca cgtgtcagcg ggcgcgatcc gcaaggtcgt tcctacttct
     3721 catttgcctc cttcaaggat ccggacggga acggctggtt gctccaggag atccagacgc
     3781 ggcttcccaa ccgtgagtgg aacttgacgc taccacaggc catggacact gcaaccttgg
     3841 cagggctcct cagcgagacg gccgagcatc acggcaactt tgagaagacg cacgccgagc
     3901 atcaatggtg ggactggtac gcgccatatc taagcgcccg tcagagtggc agcaatccgg
     3961 agaaggcagc agcagccgct gaccgctata tggaggaggt ccttcacgtt cttcccaaat
     4021 gatgtcattc gagtggccgc actattgcaa actcatatta gaaaaagaag gatatgttga
     4081 aatcaattct aacatattct tgattttaat tatttgttaa ctccataata gttcttcctg
     4141 aacccacata tttaaaaaat aataatgagt tagaattctt tatcagaaga gacaacatta
     4201 cccaaaaact caatattaaa gaatgcaatc aatggataag aatcaattgg tcatgattgt
     4261 tctaaattca attaactatt attactaaat tctggttata tcattttaga taataaatag
     4321 atagtatttt tttatattcc tagttttagt aatttttaaa aatataatag atcattgatt
     4381 agaatgactt atttttactt gttatatgat tttatataca atgagttata caaacctaga
     4441 taaaaacaaa tcttatttta ctaatattaa aagtatcttt agctttgact ttagcatata
     4501 tctatataga ttttatctat tagtattatg aaatcattca ttggtctgtt tgactttata
     4561 tcaatctatc agtctattaa cgaataacca atcctttagt ttatggttac aaaaaatgat
     4621 agataatact gaattactat atattagatt ttcatgaatt catatattaa aaaattagag
     4681 ccttgttgct agacgggctc ggaggtctga actgattttg acacaatttt gtagttctat
     4741 agttaggaac aataaattat ttcttcattt tccaatttta ttatgtacat catcatatct
     4801 ttttagaccc acaattttta cagtattaaa caagcctagt cctgcctcaa tgcataggct
     4861 ctctatatca tgagcacttt ctgcttcgaa aattatcatc cattcatgtt caaggactga
     4921 catgtagaa
//
LOCUS       DASVEV010000187         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1243876, whole genome shotgun sequence.
ACCESSION   DASVEV010000187 DASVEV010000000
VERSION     DASVEV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243876"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1694)
                     /locus_tag="VF222_08495"
     CDS             complement(198..1694)
                     /locus_tag="VF222_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7142503.1"
                     /translation="MSIPGYKELLGQEHHDDKNSENTLFLIMGPGRSGKTTYCKTFFE
                     SILKIGNSGIFLSSTLTKRQYQNLFNSLSSILDSNSFFINPFLREQINSSETNNSEFD
                     NSLVDIKKEIDKIIISSKQEQKNHGMSEKEEKNNLSSLPQKRICMVIDSLSHLFNIFD
                     EKDVLRFINTLSFSLKNNNILSIFTIDDTVVEQSIIDKIRPLFDGVLQIKLDEKEDEI
                     IRKIKFLSLTGFSSNYPSWVKFELDKNSLLSFPNSSSLICSVCKASIKTEPVFYLDMA
                     FHENHLELYKKLIGIYGNTRISDLGPSSVINANFFFIDIVGLSSPSLSVRKQIEKIEF
                     LNNFVISSEIFRKNIDKKVLPTGDGMAIGFTSNPEAPLELSMQLHNKLRRHNIGKDKE
                     SSLAVRIGIATGPVFIVNDANNNQNIWGPGIIFARRVMDIGDDNHILLDGGLAHSLIT
                     LDDKYKEIIHYIGDYQIKHGQIIKLYSAYQENIFGNKDIPVKMKCLPV"
     gene            1949..>2676
                     /locus_tag="VF222_08500"
     CDS             1949..>2676
                     /locus_tag="VF222_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7142504.1"
                     /translation="MKKCIAFHSYKGGTGKSTISANISVLLVKMGYNVLLIDMDVYAP
                     TLQTYFSQEPTFWINNFLFNTAEFENVIYDLTRLIRNFPSTKEPQKELGKFFVAFSNS
                     SKEEITKLDGTLRQDSSKMELLRKFILIKEEMAYQNDIDYIIIDTSPGIRYWSINSLA
                     IADLILLSLKMDSIDIEGTKKMANEIYSAFTKLGTKSLLLFNRAAGYCLPPNLIQETS
                     YAPEANYINVMNEQSNITDKFKKDV"
BASE COUNT          948 a          384 c          362 g          982 t
ORIGIN      
        1 tattcatctt cctattctct tacaataacc aatatagaat attacaagga acttcctaac
       61 actaattaga gaatagatta taggattttt taactcagtg aattaaataa atgtaaaaac
      121 aattataaaa gttcaaggtt attatttatt aaaaaatttg atatgtattt ttagcaaata
      181 aaatttaaca tgagagttca aactggtaaa catttcattt ttactggtat atctttattt
      241 ccaaatatat tttcttggta tgcagagtat agctttatta tttgaccatg tttaatctga
      301 taatcaccta tataatggat aatttcctta tatttatcat ctaatgttat caaagagtgt
      361 gctaggccac catcaagtag gatatggtta tcatctccta tgtccattac ccttcttgca
      421 aaaataatcc ccggccccca aatattttga ttattattgg catcattaac tataaaaact
      481 ggaccagttg ctatcccaat tctaacagct aaagaagatt ctttatcttt accaatatta
      541 tgtcttctta atttattatg taattgcata ctcaactcta aaggagcttc aggattggat
      601 gtaaatccta ttgccattcc atcaccagta ggaagcacct ttttatctat attttttcta
      661 aagatttcac tagatattac aaaattgttt aaaaattcta ttttttctat ttgttttcta
      721 acagacaagc tagggctaga taatccaaca atatcaataa agaaaaaatt agcatttata
      781 acactagatg ggccgagatc tgatattcta gtgtttccat agattcctat taatttttta
      841 tataactcta aatgattttc atgaaaagcc atgtcaagat aaaagacagg ttcagttttt
      901 atcgatgctt tgcaaacgct acaaataaga gatgaagaat ttggaaatga taataaagag
      961 tttttatcta attcaaattt tacccatgaa ggataatttg aactaaatcc agttaatgat
     1021 aaaaatttga tttttctaat tatttcatct tctttttcat ctagttttat ctgtaaaacc
     1081 ccatcaaaga gaggacggat tttgtctatg atagactgtt caacaactgt atcatcgatt
     1141 gtaaaaattg aaagtatatt gttatttttc aaagaaaaag acaaagtatt gataaatcgt
     1201 aaaacatctt tctcatcaaa aatattaaac agatgtgata gagaatctat aaccatgcat
     1261 atcctttttt gtggtagcga agataaatta tttttttctt ctttttcact catgccatga
     1321 tttttttgtt cttgttttga ggatataata attttatcta tttctttttt gatatcaact
     1381 aagctattgt caaattcaga attgtttgtt tctgaagaat taatttgttc tctcaaaaaa
     1441 ggattaataa aaaaagaatt agaatcaagg atagaagata agctattaaa taaattctgg
     1501 tattgtcgtt tggttaatgt tgaactcaag aaaataccac tattaccaat cttcaaaatg
     1561 gattcgaaga aggttttaca atatgtagtt tttccagatc taccagggcc cattattagg
     1621 aagagagtat tttcgctatt tttatcatca tgatgttcct gtccaagtaa ctctttatat
     1681 cctgggattg acaatatgtt aataattaca ttagatttat ttaaagttag atatgattct
     1741 ttaataaaat tcgtatatta aaagacagct agatgatcaa tattaataat ctattttgtg
     1801 tcatttaacg ttactatatt tttatgaaat gtataaccat taaataaaaa atccccgtca
     1861 tagtttttcg ctatttataa tgcgttaatt ttctttgtat caaacgaata aaaaatattc
     1921 ttattacgct tgtattaaag ttaaaataat gaaaaaatgt atagcttttc attcttataa
     1981 aggtggaaca ggaaaatcta caattagcgc taatatttcg gttttactgg ttaaaatggg
     2041 atacaatgtt ctccttatcg atatggatgt ttatgctcca accttacaaa cttattttag
     2101 tcaagagcca acgttttgga tcaataattt tctatttaat acagctgaat ttgaaaacgt
     2161 tatttatgat cttactcgcc taattagaaa ttttccctcc acaaaagaac ctcaaaaaga
     2221 attggggaaa ttttttgttg cgttttcaaa ttcctctaaa gaagagataa caaaattgga
     2281 tggaacatta agacaagatt cgtcaaagat ggaattactg cgtaaattta ttctaattaa
     2341 ggaagaaatg gcttatcaaa atgatattga ctacattatt atagatacta gtccaggaat
     2401 acgttattgg tccataaact ctctagcaat agcagatctg attctattat ctttaaaaat
     2461 ggatagcata gacattgaag gtacaaaaaa aatggctaat gaaatttatt cggcttttac
     2521 aaaattaggt accaagtctc ttttgctttt caatagagct gctggatatt gtcttccccc
     2581 aaacttaatt caagaaactt cctatgctcc ggaggcaaat tatataaatg tcatgaatga
     2641 acagtctaac attactgata aatttaaaaa agatgt
//
LOCUS       DASVEV010000188         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_663247, whole genome shotgun sequence.
ACCESSION   DASVEV010000188 DASVEV010000000
VERSION     DASVEV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663247"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF222_08505"
     CDS             <1..492
                     /locus_tag="VF222_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142505.1"
                     /translation="TDFADRYEKNIYPLLRAGYIVLADRYIYTALARDTVRGCDQSWV
                     RNMYSYARKPDLVFYFRIPVEIAVTRIISGRPKLKHYEAGMDLGLSKDEYESYKIFQG
                     RIVDEYESMVSDENFIVIDGTLDIEKQQNTVRRNVLRTLKLKNQIRGNIARLSQLETN
                     TDE"
     gene            494..1270
                     /gene="tmk"
                     /locus_tag="VF222_08510"
     CDS             494..1270
                     /gene="tmk"
                     /locus_tag="VF222_08510"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7142506.1"
                     /translation="MISRKLTKNKDYSTTKKESNNKIKFINHSGIKYLKDIEINGSFI
                     VIEGPDASGRSTQIQKITEKLEADGHAVVNAGLKRSDLISQGIIEAKRNFQVGRRTMA
                     LYYAADFADQLENKIVPALKAGFIVISDRYIYTLIARSSVRGINKNWLHQLHGFAIKP
                     DLIFYLNVDPFNLIHRVFKKNKSLDYYESGSDLGISADIFDSFIKYQYLLKKEFTLMQ
                     KKYGLIDVDGNKSIEDIYHNIQTKISGYLDQNKKNETLFR"
     gene            complement(1651..2553)
                     /locus_tag="VF222_08515"
     CDS             complement(1651..2553)
                     /locus_tag="VF222_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7142507.1"
                     /translation="MLQCFHVRYDSNTHANNCYENYEKVLAKLDKYLENPNEDNIHHM
                     RTSLRRLEAAYQSSPKQIRNKKKIKKFASSAKKLFRINSKIRDVDIILKMLANEGKMP
                     QDKLEEFKNHNEYDKQKYLKQARIIALDLRRQIIPNFYDRKKINSNFTRKSRKRLLKM
                     VRKLKSKIERKLPIVINNEDRILVLHELRKDTKKLRYLIELVTKRDYERAGERSTGDE
                     GSDEQLGENHNRILRQLEKIQGMLGEIHDYDVTIDYLNQQHLSNNSLISDIIENMMKV
                     RKKRFNEFVDYNKPTNLDVKCVLK"
     gene            complement(2790..3320)
                     /locus_tag="VF222_08520"
     CDS             complement(2790..3320)
                     /locus_tag="VF222_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7142508.1"
                     /translation="MKDKKCNKILLILRHAKSSWKNKKLEDYDRPLNKRGKSEAIKMG
                     KYLKEMAILPNLIITSSALRAIETTKYVCKHAGYKNLIEVDSSLYGSDIDNYISVIST
                     AFKTKDRVLIIGHNPALEELVSLITNKIMILPTCTLVQINLILKNRDIFSLLDIATSE
                     IVNIWRPKDPMESNNL"
BASE COUNT         1223 a          534 c          591 g         1305 t
ORIGIN      
        1 acagattttg cagacagata tgaaaagaat atctatcctt tactcagagc gggctatatt
       61 gttctagcgg acagatatat ctacactgct ttggcgagag atactgtaag aggatgcgat
      121 caatcatggg tgcgaaatat gtacagttat gcccgaaaac ctgacctagt tttttatttt
      181 agaataccgg ttgagattgc tgtgactaga atcatttcag ggagacctaa actgaagcac
      241 tacgaggctg gaatggatct gggattaagc aaggatgaat atgaaagtta taaaatattt
      301 caaggtagga tagttgatga gtatgaatca atggtgagtg atgaaaattt catagtaata
      361 gatggaacac ttgatataga aaaacaacaa aatactgtaa gaagaaacgt tctaaggacc
      421 ttgaagttga aaaaccagat tcggggaaat attgctagat tgtcacaatt agaaacgaac
      481 actgatgaat aagatgattt caagaaaatt aactaagaat aaagattact ctactaccaa
      541 gaaggaaagt aataataaaa ttaaattcat taaccattcc ggaataaaat atctcaaaga
      601 tattgagata aatggatcat tcatagtaat agaaggccca gatgcttcag gtagaagtac
      661 ccaaattcag aaaataaccg aaaaattaga agctgatgga catgcagtag ttaacgccgg
      721 cctgaaaagg tctgatctaa tttcacaggg aataattgaa gcaaaaagaa actttcaggt
      781 agggagaaga actatggctc tctattatgc ggccgatttc gcagatcaat tggaaaataa
      841 gatagttcct gcgctaaagg ccggttttat cgtaatttct gatcgatata tttatactct
      901 gatagcaagg agttcagtta gagggataaa taaaaattgg ttgcaccaat tacatggttt
      961 tgctatcaaa cccgatctta ttttctatct aaatgtggat ccatttaatt tgattcatag
     1021 ggtattcaaa aaaaacaaat cacttgatta ttatgaatca ggatcagatc tgggtatatc
     1081 tgcagacata tttgattcct tcattaaata tcagtactta ttaaagaaag aattcactct
     1141 gatgcaaaaa aaatatggtc taatagatgt tgatggtaat aagagtattg aagatattta
     1201 ccataatatt caaacaaaaa tcagtggata tctcgaccaa aataaaaaaa atgaaacatt
     1261 atttagatag acaatttgta tttttatttg agcgcttcat tgatgtatta caactagaga
     1321 ttttatttga tatatagaat atattgacca ttaggacaat cattaaatct gttacctcaa
     1381 tacaataaac atggtgagct tgaggacaaa actatatact gagggaggaa aattatgcat
     1441 gggttgtttt catcaaaggc gtattcatag atataatatg agtcaacaaa ataatcgtct
     1501 tctgtgtaat catcacactt gtagttgtac ccaattcata gaatggaatt gataatctgt
     1561 tgtcataatt tttgagatta aagtgataaa gaaaatatta tgatactgtg actgggaatc
     1621 gttctttatt tacaattatt actatctatc ctattttaaa acgcatttca catccaaatt
     1681 cgttggttta ttataatcaa caaattcatt aaatcttttc tttcttacct tcatcatatt
     1741 ttctattata tcggagatta gagaattatt tgatagatgt tgttgattca gataatcaat
     1801 ggtaacatca tagtcatgga tttcgccaag cattccttga atcttttcaa gctgtcgtag
     1861 gattctgtta tgattttctc ccagttgctc gtcggagcct tcatctccag tggacctctc
     1921 accagctctc tcataatctc tttttgtgac aagttctatt agatagcgca acttttttgt
     1981 gtcttttctg agttcatgta ataccaatat tctgtcttca ttattaataa cgatcggaag
     2041 ctttctttct atctttgact ttaattttct taccatcttt agcagtcttt ttcttgactt
     2101 tcttgtaaag ttactattaa ttttttttct atcgtaaaaa tttggaataa tttgtcttct
     2161 taaatctaaa gcaattatgc gtgcttgttt aaggtatttc tgcttgtcat attcattatg
     2221 atttttaaat tcttctaatt tatcctgcgg cattttaccc tcatttgcta acatttttag
     2281 aattatgtcg acatctcgta tcttgctatt gattctaaat agctttttag ctgaacttgc
     2341 gaatttcttt attttttttt tgtttcgtat ttgtttggga cttgactgat atgctgcttc
     2401 tagtcttcga agggatgtac gcatatgatg gatattatct tcatttggat tttctagata
     2461 tttgtctaac ttagcaagaa ctttttcata attctcataa cagttattag catgggtgtt
     2521 cgaatcatat cgaacatgga aacactgtaa catacaatta gatatcgtta ttgtaatatt
     2581 aagtgttata aagctcggat ggattcttta ttgctagttt atttaaatta tagatagtag
     2641 atttttccgc ccctcagtaa atgagaatca tgaaataaaa ttaatgttgt atttattttt
     2701 aaataataat cttgacatat ttatttgatg agattaaaag gctaccgttt tgtttaattt
     2761 gtaattgagc attttcaaat tatataatcc tacaaattat tactttccat tggatccttt
     2821 ggccgccata tattcacaat ttctgacgta gcaatatcga gcaaactgaa tatatctcga
     2881 tttttcaaaa ttagattaat ttgaactagt gtgcacgttg gtaaaatcat aatcttgttt
     2941 gtaataaggc tcactaattc ttctagtgct ggattatgac caattattag aactctgtct
     3001 ttagttttga atgcggtaga tattacagat atataattgt caatatcact tccatacagt
     3061 gaggaatcga cttcaatgag attcttatac ccggcatgtt tacatacata tttagtagtt
     3121 tcaatagctc taagggccga ggaggttatg atcaaattgg gcaaaatagc catttccttt
     3181 aaatattttc ccatcttaat agcctcagat ttacctctct tgttgagtgg tctatcatag
     3241 tcttccaatt ttttattctt ccaacttgat tttgcatgtc ggagtattaa caaaatcttg
     3301 ttacatttct tatctttcat atcatacatg aaaaattaaa gataacatgt taaaaaggta
     3361 cttgcaaatg gatcaagaat cttattcaga aatatgaaca tttccataac atcttttcct
     3421 attcgtgcta attcataatt tgttgtttat ttaagattaa ttatagttat tatttttatt
     3481 acctacatat tattcaaaaa atcgatcaac taaaaaacaa tgatatacgg agaagataaa
     3541 ttgaaaagca aagaaaaaaa ttggttcata ttcaaaatat tattacagaa ttatttgttg
     3601 tcgatgatag aatagaatac tgaggagatg taaacatttt ataacaaata gct
//
LOCUS       DASVEV010000189         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1635465, whole genome shotgun sequence.
ACCESSION   DASVEV010000189 DASVEV010000000
VERSION     DASVEV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635465"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..331
                     /locus_tag="VF222_08525"
     CDS             68..331
                     /locus_tag="VF222_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142509.1"
                     /translation="MRDNYGKIIKSENLINKKAKGIDNIDLGEIQKITSEYIITEKKD
                     TDINKDKDKIFIPRNLLERWDEDNVWFHVTEEEKESLKENQNQ"
     gene            1276..1875
                     /locus_tag="VF222_08530"
     CDS             1276..1875
                     /locus_tag="VF222_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142510.1"
                     /translation="MKPIFFSSFLLTVLLLTFSIVFLTNFNEVFGSNEKSKDNKENRL
                     NCGEIGPHLLVKVVTEQGTGKLLADFKRLANDKVMVSETSYRDQNECFEDTIELKGGE
                     RIRLTFMECSSNNNCAIKEGPFNDIVILSNLTEDEINYDNIKTSSFGETRTFEPDDNL
                     KFKIPHNIHRNFNSLIITSQFGKDEHQRDFVLTDNIIVR"
     gene            complement(2313..2864)
                     /locus_tag="VF222_08535"
     CDS             complement(2313..2864)
                     /locus_tag="VF222_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142511.1"
                     /translation="MRIFIALLTIVLSVVVSTCSLYNPQQLIISEPIDKNLTSLTSSE
                     SIHHSVLQVEQIKTIPDKVHVGDEFSINATVKNTGMYPVTFIANLCDSELYAEFDNKV
                     EEIRAPECKMMSEPVILNAGEQVIVQRISGISGLTYKALEEGQSDAKVILPYKINISE
                     IVTVDSLVGNKFNVTINQEPSNL"
     gene            complement(3125..>3376)
                     /locus_tag="VF222_08540"
     CDS             complement(3125..>3376)
                     /locus_tag="VF222_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7142512.1"
                     /translation="PESTLNDSNSMFPVHGAITPPSIVHFALEINIADYEKWKDLLQK
                     NQINIEKELKIGNSKSIYFRDPSGNVVELITENAWPVDN"
BASE COUNT         1305 a          430 c          477 g         1164 t
ORIGIN      
        1 aaaaatatca attactttat gaatttaaag catataccgc tatataaaat gcagaatcat
       61 tacaattatg agagataatt atggtaaaat tataaaatca gaaaatctca ttaataagaa
      121 agcaaaaggt attgataata tagaccttgg agaaattcag aagattactt ctgaatatat
      181 aataacagaa aaaaaagata cagatattaa caaagacaaa gataaaatct ttattccaag
      241 aaatttatta gaacgctggg atgaagataa tgtatggttt cacgtaacag aagaagaaaa
      301 agaatcactt aaagaaaatc aaaatcagta atttttatcc attttatacc attttaaagt
      361 attgagtaat tttacaaaat taaaattact tttctacaat aattcttatt aaaatgaata
      421 taatcaatag gaaaatgatg ttagatacta ttaatgcctt tatttgttat taataatctg
      481 tttaaggata aattaatgat aactaaatcg gccaaacgat tttaagaaaa caaaatgaaa
      541 taataaagaa aaaaacgata tgtttaagta attatacaat ggttagtgtt aaggaatcta
      601 ttaaaataga tctcattggg ccaatgattg gtaaagggat tcacccatca ttggcctaac
      661 tacatcatca tttgtgtgta agatttttgt atgatacaat ctaaacgtta gtagacgaat
      721 tcgtcattgc tctgacttgg cagcaaaact tgtactcaat tataatactc tgaatgccat
      781 tcaattgtat ttaagaaaga ttttattcta ataaattcta atgaatggtc cagatggtaa
      841 aaggctttca aattccgcta gtcctctttc gatatttgtt ctttaattgt aattacttaa
      901 aaagagtgaa agcaaaaata ataatgaagt agaacaagtt tatgaagaac taattcataa
      961 tcataatttg tttatagagt taaataataa ggtacagaat ttagtaattt tagaagattg
     1021 aaaatttttc tttaagcaga ttattacaat ctattaacat gaataaagat gcaattagtt
     1081 caagcaaaat gtaaaaatac tccagttgac ggttttaatt gaattatagg cttatgaaat
     1141 tccaaagaaa taaggacata attaggaaga ttaaagcaat aattcaaatt tgtaataatt
     1201 ctttgttata ttttcattat aaataaataa taacaaataa aaaaatttaa ctaactgaat
     1261 attttataac aattaatgaa accaattttt ttttcttctt ttttgctgac tgtattacta
     1321 ctaaccttca gtatagtatt tttaacgaat ttcaatgaag tatttggctc gaatgaaaaa
     1381 agtaaggata ataaagaaaa tagattaaac tgtggtgaga taggtcctca tctacttgta
     1441 aaagttgtaa ctgaacaagg gacaggaaag ctcctagctg attttaaacg tttggctaat
     1501 gacaaagtaa tggtaagtga gacttcttat agagatcaaa acgaatgttt tgaagataca
     1561 atagaactaa agggaggaga aaggattaga ctaactttta tggaatgtag cagcaataat
     1621 aattgtgcta taaaagaagg cccattcaat gatatcgtaa ttttgtcaaa tctaactgaa
     1681 gatgaaatta attatgataa tatcaagaca agttcatttg gtgaaactag aacttttgaa
     1741 cctgatgata acttgaaatt taaaatccca cataatatac atagaaattt taattcgttg
     1801 attataacat cacagtttgg gaaagatgaa catcaaagag attttgtcct gacagataat
     1861 ataatagtaa gatagaccat ggtgaataaa ccagcagcaa tattttttat attaggtaaa
     1921 gttaaggaga aggaagaaca accccttcta attaagaata aacttatttc aaacagtaca
     1981 aaccacaaaa agacaacttt tagatgcttt tgtacatcta aataatatta tcatatacta
     2041 tcatatttcc agaaatatgc taactaatcc ataataaggt aattttatag taaaatttac
     2101 taagtataca aataaacaaa tacccaaaaa gaagaagcaa caaaaacaaa tgtcagtcag
     2161 tcatcgacct gaaatgcata caagaattta ttttttgctt ttaaattatt atgcgtttgt
     2221 ctatagtttt tgtaaaacta tgacttgcta catattactt atattgataa aatcttataa
     2281 tcattttatt gtaaaaagat gtagatatta aatcacaaat tagaaggttc ttggtttata
     2341 gtaacattaa atttattgcc aactaaacta tctactgtta ctatttcaga tatatttatt
     2401 ttatatggaa gaataacctt tgcatcagat tgcccttctt ctagggcttt atatgttaaa
     2461 cctgatatac ctgaaatacg ttgaactatc acttgttctc ctgcatttaa tataactggt
     2521 tctgacatca tcttacactc aggagcacgt atttcttcga ctttattgtc aaattctgca
     2581 tacagttcag agtcacaaag atttgctatg aaagtgacag gatacattcc cgtattttta
     2641 acagttgcat ttatactaaa ttcatctcct acatgaactt tgtctgggat agttttaatc
     2701 tgttcaactt gcagtacaga atgatggatt gattctgaag aagtcaaact agttaaattt
     2761 ttatctatag gttcagaaat aattagctgt tgtggattat agagtgaaca tgttgagaca
     2821 acaactgaca atactatagt tagtagtgct ataaaaattc tcatgaattt aatgagagaa
     2881 tgttatctat aaagagttct tgccataatt ccaaaaagtt ttatggattt aaatatcaaa
     2941 ttatatttta taaaatatta ctataaaatc cttttatttt agttccttgt attctcttta
     3001 tttctttcca atcctaatta ggtttctaat agatatctac ttttgagtta tgatttatag
     3061 catatctctc ttttaagtta ttggcaaacc tatactacag acaaagtatt ggaagtaata
     3121 gatattaatt atctactggc caagcattct cagttatcaa ttcaactacg tttccagaag
     3181 ggtcacgaaa ataaattgat ttgctatttc caattttcaa ttctttttca atattaattt
     3241 ggttcttttg taaaagatct ttccattttt cataatctgc tatattaatt tctaaagcaa
     3301 aatgaacaat tgagggtggg gtaatagcac cgtgtacagg aaacatacta ttagaatcgt
     3361 ttagtgtact ttcagg
//
LOCUS       DASVEV010000190         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_665047, whole genome shotgun sequence.
ACCESSION   DASVEV010000190 DASVEV010000000
VERSION     DASVEV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665047"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(948..2099)
                     /locus_tag="VF222_08545"
     CDS             complement(948..2099)
                     /locus_tag="VF222_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEX7142513.1"
                     /translation="MSINQQKNTSQNPISIRELSADEPLGVLEPVVYFNGAMPTGVTV
                     SQQGRIFVNFPKWGDDVQFTVAEIKNGKAVAYPDEVFNQTDPDDLAKALVSVQSVVVD
                     PMDRLWILDTGSPLFQPTKYGGPKMVCVNLTNDQVVKKILFPQDVAFPTTYLNDVRFD
                     LRLGSEGIAFITDSSQKGPNGIIVVDLASGESWRRLNDHPSTKAENLQTFLPIVEGRP
                     FLEHQPDGSIKQGASMGSDGIAISEDGSRLYYCPLGSRKLYSVDTHALADRSLDERKV
                     VATVTDEGDKGGAADGLESDATGYIYSTNYEHNSVLRRQRNGGEWETVVHDPRLLWPD
                     TLSVAADGYLYVTANQLHRQARYQKGHDLRHKPYTLFRVHINSSPVLLR"
     gene            complement(2454..2600)
                     /locus_tag="VF222_08550"
     CDS             complement(2454..2600)
                     /locus_tag="VF222_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142514.1"
                     /translation="MPRGVKKGVNTKKFDSCVEKVKDTSGKKVNPYAVCNATMSRKTK
                     KKKD"
     gene            2989..3114
                     /locus_tag="VF222_08555"
     CDS             2989..3114
                     /locus_tag="VF222_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142515.1"
                     /translation="MSDIDPDQTKCKECGISFDTTEGLEQHYELEHKWKNDKVQL"
     gene            3454..3654
                     /locus_tag="VF222_08560"
     CDS             3454..3654
                     /locus_tag="VF222_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142516.1"
                     /translation="MNIIKNVTFGLIGSTYGATGLGVIDGGQILAIASVALGGAAMVV
                     ALIVRNHLQKKKLDTQIAIEMK"
     gene            4044..>4291
                     /locus_tag="VF222_08565"
     CDS             4044..>4291
                     /locus_tag="VF222_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142517.1"
                     /translation="MKFLITTEVFETIHKEEDLKRQTELIKSQIKTIMNSGKLLTGGQ
                     FADKRGHFFVIEAADELELLELLNRNILDSCKVESHPIV"
BASE COUNT         1551 a          604 c          677 g         1459 t
ORIGIN      
        1 gcagatgtgt aaggagcaca agacacagat aaaatataat ttagatactc atcggtttta
       61 ttgtctggac aaaataagag aataatatcc ttctttattc agaaaagatt cattcaagaa
      121 tttgaattct tgttaaatga aaaaatatca gggaagtaca aattgtatta aaataggtta
      181 attctcctcc ggttgtgcgc ttgataaact taatagtaca caaacagaat aaactcaaca
      241 ataagtttca tttaaaagta taacatacta ctactaatcc ggagaatggg tattaacacc
      301 ttgcatgaac attctccaga tagtgtatgt gtggatttaa tccagaatag cattgggaat
      361 ttatttatta tagtttgcta ctaacctttc tcttttcata ttctataaca aaagaaatga
      421 ggatagattg ttattattat aatatagtgt gttgtcaaaa agtaacattt ttcagcattg
      481 atattactaa taataattag caattgaata atctaataag tattaaataa taaaaattat
      541 atattatatg ttcctgaata attcattata tctaatataa taattagaat cttcagtgat
      601 tatcatgcta atgtcagcaa ttatgttcgg acttttgtta ataggactat taataatcat
      661 aattttagtt aacatgaata ttttttcaaa tgtaattgct gtagatataa atccatatac
      721 gaattactac tcacaaattc tgtatatata aataattatg atgataataa atttcctttt
      781 agttcatcat cattttaata taaaaaatag actagttagg aaatagattc tattttattt
      841 ctatattatt aactttataa ttttatctat taaatgtaat agatattaga aataggaatt
      901 agttatggtt tattattata tttagtagta aattatcgag ctgcatctta cctcaggaga
      961 accggtgaag agttaatatg tactcggaac agtgtgtaag gtttatggcg taaatcgtgt
     1021 cctttttggt agcgtgcctg gcgatgcaac tggttggcag taacgtagag gtatccatcc
     1081 gcggctacag agagtgtgtc aggccataag agacgcgggt catgtactac agtttcccat
     1141 tctccaccat tacgctgacg acgcagaaca gaattgtgct cataatttgt agagtaaata
     1201 taaccggttg catctgattc cagcccatca gcggctccac ccttatctcc ctcatcagtc
     1261 acagtagcga cgacttttcg ctcgtcgagg gatcggtcgg ctagtgcgtg tgtgtccaca
     1321 ctgtaaagct tacgactacc aaggggacaa taatatagac gagaaccatc ctcgcttatt
     1381 gcaatgccat cagaacccat gctagcacct tgctttattg atccatcggg ttgatgctcg
     1441 agaaagggtc tcccttctac aatcggaaga aaagtttgta agttctctgc cttagttgag
     1501 ggatgatcat tgaggcggcg ccaactctca ccagaggcca agtccacaac gatgattcca
     1561 tttggtcctt tttgggatga gtcggtgatg aatgctatac cttcgctacc tagtcgtaga
     1621 tcaaaacgta catcatttag ataagtagtg gggaaagcca cgtcttgagg aaagagaatt
     1681 ttcttaacaa cctgatcgtt ggtcaaatta acacagacca tcttagggcc tccatatttt
     1741 gtcggctgga aaagaggact acctgtatcg agaatccata gacgatccat aggatcgaca
     1801 actacggatt gcactgatac cagtgccttg gcaagatcat cggggtcagt ttgattaaaa
     1861 acctcgtcag gataggcaac cgccttgcca ttctttatct cagcaaccgt aaattgtaca
     1921 tcatcacccc actttggaaa attgacaaaa atacgtccct gttgtgatac agtcacacct
     1981 gttggcattg ctccgttaaa atacacgact ggttctaaca cacccagagg ctcatctgct
     2041 gataattccc gtattgaaat aggattctgt gatgtatttt tctgttgatt gattgacatg
     2101 gttatttgct cctttttcta taattgctct attaatttcg attgtgcaaa tgtgttatta
     2161 ttatagacca attgggatat caaaaaaaac tgaaaaatag gaagtacagt atttactatt
     2221 tctttgtaat aaggaatatt attcttattc attgtgttac tatatttagg taatatatta
     2281 tataacagtt atttttgcac atgttttaga aatttcgtaa ttaattttaa atttagtaca
     2341 aataaattgt aacaagttat tgcaattata atttaagggt cttttttcca taactgaatt
     2401 aataaatatt attaaaaaaa taaactatat agtataaatt atattatttt actttagtct
     2461 tttttctttt ttgtttttct actcatagta gcattacata ctgcataggg attgactttc
     2521 tttcctgatg tgtctttcac tttttcaacg cagctatcaa attttttggt atttactcct
     2581 ttcttgacac ctcttggcat attatatata agtcattata tttaattaag attgtagaaa
     2641 aatattttat aaaattactt taaaatgaag aatactgtag tagttaaact acagttattt
     2701 tttattgtcc actattattt tttagcagaa cagtgttcat ataaatttat taaatattca
     2761 attcaataga agcgaaatat ctccaaagaa ttataataaa taagttattc gaatgtgcta
     2821 atctagagta cgaaaattat tttaacacaa tttgttatta acaaatagta aaaaatgaaa
     2881 tacaatatca tgaaacctag caagacaaaa ttcaaatata ttgcatgata aataaaagat
     2941 aaataaaaaa cataatataa aaatacaaag actattaatt atttattgat gtcagatata
     3001 gatccagacc aaacaaagtg caaagaatgt ggaatatcat tcgatactac tgagggatta
     3061 gaacaacatt atgagttaga acataaatgg aaaaatgata aagtacagtt atagccattt
     3121 aaatctaatt ttaaaatata ttaaaaaaag aaatacaaaa ctgaaggtat ataaatcaac
     3181 tgaaggcata aatcaagggt ttaaaatcca agaataaatt taatatttat atcattttat
     3241 tttataattg ttaaataaaa aaaattgcat ctaaacaagt ttggtgataa taattttgta
     3301 tcttaaacaa ttacaattaa gcataacatt ttcactttaa actataaaat tgttcaagtt
     3361 ctgataaatc ataatttata gttagaaaaa aaagttagca ctataaatat aattagtccc
     3421 tagtttatat attacggaat ggtataatac tatatgaata taataaaaaa cgtgactttt
     3481 ggtcttatag gcagtacata tggagcaaca ggtttaggtg ttattgatgg aggacaaata
     3541 ttggcaattg caagtgtagc attaggcgga gcagcaatgg ttgtcgcttt gatagttaga
     3601 aaccacttac aaaaaaagaa attagataca caaatagcaa tagaaatgaa ataatagata
     3661 aaaaaaagta gcctagataa ccaaatttag aataattttt tttattatgg tgaagtgttt
     3721 tactcgctat aattttttta taaaactaat attccaagta ttataataaa caattaaaat
     3781 aacttatcta gttaattata tacgggttct taggtttttc aagtataaca tgaaaaattg
     3841 agatcgggat cggaaaacta gtatagctgt ctggtaactt atttgaagaa aattttgcca
     3901 tgtgcaaaaa aatggtatat agaattagag gcgattgaac cattctatca tctatatctt
     3961 ttattataat tttttatttt ggtattcgta tgttagatga tactatgata aaatataaat
     4021 taataaatta ttatactaaa ttaatgaaat ttctaataac taccgaagtt tttgaaacta
     4081 tccataaaga agaagattta aaaagacaaa cggagttgat taaatcacaa attaaaacaa
     4141 ttatgaattc aggaaaatta ctaactggag gacaatttgc agataaacgt ggtcattttt
     4201 ttgtaataga ggcagcagat gagttagagt tattggaatt gctaaataga aatattctag
     4261 attcatgtaa agtagagagc catcctatag t
//
LOCUS       DASVEV010000191         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1637298, whole genome shotgun sequence.
ACCESSION   DASVEV010000191 DASVEV010000000
VERSION     DASVEV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637298"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(988..1506)
                     /locus_tag="VF222_08570"
     CDS             complement(988..1506)
                     /locus_tag="VF222_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142518.1"
                     /translation="MSFRDISNIIKAYEKKARLQGTKKEEQENNQKIRKVSLSSQAFI
                     LYQEGKKPSEVKVLLDIPFKKTMVYWSQYLKSIRMFESFEFYQEFSYDIPTFLSINNL
                     IKRNNISGNNIVNVLRASTDIINLNQTITNLKAEINRLEGKRISLSYYSHSSYSLQPL
                     PLNKPNYNYYRY"
     gene            2047..2271
                     /locus_tag="VF222_08575"
     CDS             2047..2271
                     /locus_tag="VF222_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142519.1"
                     /translation="MIIKKSVIGIIVLAFISIILTTGSPFQIGTNLVENNGLLYIKYF
                     GLQAYADDDDDDKDIEEYNKEADEQHDDED"
     gene            2384..2764
                     /locus_tag="VF222_08580"
     CDS             2384..2764
                     /locus_tag="VF222_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142520.1"
                     /translation="MSFWRCQIKAIGGSICTSFVNPHQLNLFLNGTFNREYYVFSTFN
                     VFVYNYYPFIYQHAGDAKNPYLAQYSAVVEDSPVITSWISMKINILIKNIPKPLNTNF
                     NTFLIEFFLASVDNILKDIEYYSI"
     gene            complement(2935..3123)
                     /locus_tag="VF222_08585"
     CDS             complement(2935..3123)
                     /locus_tag="VF222_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142521.1"
                     /translation="MEDSKLKFVVHGRKEGDKFVLEGITNYISQSLDSFDDKTIIVTF
                     EKPVQTNTIKLDVGNLFN"
     gene            complement(3282..4142)
                     /locus_tag="VF222_08590"
     CDS             complement(3282..4142)
                     /locus_tag="VF222_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142522.1"
                     /translation="MVEKGYIKCLYIFIGIAFAVSILYVSSSYGSAFAQKNDKEEKED
                     KDKLIVKANINLKNVDMKNTKFIRVIGFINGEEVKQDIPISSIDKTKNKLDVELKVNK
                     KNDIVEASTPDEFFVCAYQVGDVENNYNSLAKFDCNEGDILSTSKPTPARLFQLGSHV
                     YEKTQAVYGANVNNTGSGSNIDKVKIKILAPLADKKDTKKLVIAAMIKGQIQSEVIED
                     VQAELDKSKGSTIQRTFTFDRDTDIGKIQFGDRYHACVASDDLSPPEGQECEKRVIKH
                     FDKSNSLPAR"
     gene            complement(4451..4624)
                     /locus_tag="VF222_08595"
     CDS             complement(4451..4624)
                     /locus_tag="VF222_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142523.1"
                     /translation="MNLKPIKTIIQFAFSGFSLFFIAMLFFILVHDTLTGESSVTTEY
                     CAKYGFLASPDCW"
     gene            complement(4825..5121)
                     /locus_tag="VF222_08600"
     CDS             complement(4825..5121)
                     /locus_tag="VF222_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142524.1"
                     /translation="MKQLKESKSMETQKLYQMTPKEVYVSSIEIELPYTPKDVQALGG
                     LKVEFVKYQVMKYLNRLTETIRFSTRRYWIKMISEKDIEIYDIEFEENNSRGCN"
     gene            5431..>5970
                     /locus_tag="VF222_08605"
     CDS             5431..>5970
                     /locus_tag="VF222_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142525.1"
                     /translation="MKCHNIAVYLIIPLALFSIISLFDNVQAQTNSSSANQTTSQQQQ
                     QNQNANLTAQTLMKTDIVALKNTLMNAQLAIVDGNIKQALKDVMDLETQLVLLKPSPP
                     TKFINNIHKAIAAISTSEIDKSLNTITNIQVSILKAENLIFKAAVANPQVMQQIHTVS
                     YVVPTPQSEKIQQVDTSKNV"
BASE COUNT         2127 a          857 c          749 g         2237 t
ORIGIN      
        1 caacaaattt aggtttgatt gaaagaaaag taaaaaaaga aaaatctcct ggacgaggga
       61 aaccaaggat ttataattct ttaacaaata agggtagaga agtagtagga ttagctgata
      121 aagtaggata cgcaaagtaa ttttttaatt ttaaaagtta acaacttaaa tatagaatga
      181 acttgaactc aaatacaaag aaaatgttca cactgtatga ttttatgctt ggaaatacaa
      241 aaacgaatta attattccat caaagatgat aagaatgaaa agaaaaaaag gccaaaaata
      301 aaattaataa ggcatatgaa aaagaaaaaa gaaaatatgt aatatttaca ataaattgaa
      361 ttttatttac aatatagcaa tacttaagcc ttatatacgc tttttgcaga tatattacta
      421 tgttctgtaa tgtatttagt aaactgtttg ggaaaaaaga gaaaaaagat ttaaaaaatg
      481 ataaaaaaaa ccttgaaaat gtataactaa tagtcgaata gataaaatct taaaaataga
      541 atttgatttt tgcttattaa ttattttttc tcttcttcat cttctttatc tacttccaat
      601 tctaactctt ttacattgaa accttgattt tcagcatttc ttagtaactc tgtcagcttt
      661 tggaaaacct cagttacatt tttttcattt cttttacgag actttagaag ataccttata
      721 aatcagtctt attttagctc cctatcttat aaatatcaaa atgttatcat atcaagaatc
      781 atttgatatt ctaattgaat ggttaaagaa gtgtgactca ataagaaagc ttgattttaa
      841 tccaagatat ctagtaaagt tgctttaaac actgccaatc agaaaagaat cccgccaatg
      901 aaacttgaaa cattgagaaa taggaattca gagatatatc atattttaca gaaactataa
      961 catctatgac atgtgttaca aataaatcta ataacgatag taattataat tcggtttatt
     1021 caagggtaaa ggttgtaaac tatagctaga atgagaataa tatgataaac taattctttt
     1081 tccttctaat ctattgattt ctgccttgag attggtaatt gtttggttca aattaataat
     1141 atcagtagat gctcttaaaa cgtttacaat attgtttcca gatatgttat ttctttttat
     1201 taaattattt attgataaga atgtaggtat atcataacta aactcctgat aaaactcaaa
     1261 agattcgaac attcttattg attttaaata ttgtgaccag tataccatag tttttttaaa
     1321 tggaatatca agtaaaactt tgacttcaga aggcttttta ccttcttggt agagaataaa
     1381 agcttggcta cttagagata cttttctaat tttttgatta ttttcttgtt cttctttttt
     1441 agttccttgt aatcttgctt ttttttcata tgcttttatt atattagaaa tatctctaaa
     1501 tgacatatga aacttctttt gcaatctccc ttattgtctt accttgtaaa tgcaattcta
     1561 tcactctttt ttccttttca aatttgttca aaaccatttc aggcatacta taataagctc
     1621 ctaaaaaaag tagttattaa atgatatcat ttgttatttt atgttatcaa ttggttaccc
     1681 ctgttaccat ctattttgca ttaaacacgg ataatatcat gaaaatatac tgtagaacga
     1741 gattgtattg gaatttataa atttataaat tataaataac ctaaaagtct taacaacaga
     1801 aatggcaagt tacattgttt ttggtgtttt attataacat ttttaattaa tttatctcaa
     1861 aatttagaat gtaaagacac cataacctaa ttacaatagc ttattttgat acgataattt
     1921 atttagatat aatgacatca tattctcaaa agtagatatt taaaaaatat caaattagaa
     1981 atttaggaaa tagaaactta aaataaaaaa tcttttatag tgaaaaatca tataataatt
     2041 acccctatga taattaaaaa gtcagttata ggaataattg tactggcatt tattagtata
     2101 attttaacga ctgggtctcc atttcaaata ggtactaatt tagttgaaaa caatggactt
     2161 ttatatatca aatattttgg tctacaagct tatgccgatg atgatgatga tgacaaagat
     2221 atcgaagaat ataataaaga agcagatgag caacatgatg atgaagatta agagaggagg
     2281 gcgatgcata atgaatactg aagtaattaa gaattttcag cttctttcaa tcttttatca
     2341 gttacatttt taatgcaata ataatttttt cagtatatat ttgatgtcgt tttggagatg
     2401 tcaaataaag gcgattggcg gctcaatttg taccagtttc gtcaatcctc accagctcaa
     2461 tctttttttg aatggaactt ttaatagaga atattacgtg tttagtacct ttaatgtttt
     2521 tgtttataat tactatccat ttatttacca acatgcagga gatgctaaaa acccatactt
     2581 ggcacaatat tctgctgttg ttgaagattc accggtcatt acatcatgga taagtatgaa
     2641 aattaacata ctaataaaaa atatacctaa accactgaat accaatttta atactttttt
     2701 aatagagttt tttctagcta gtgtggataa catcttaaaa gatattgaat attattctat
     2761 ttaattatta taatacataa gtcgaaatgt atgcaaattt tgcttagatt gttaagcact
     2821 cagattttta gtaatggctg ttaagcgata acattaacac tcatttacaa catccgatga
     2881 ttcaacacaa ccaaaaaata attctttaat tttctattct tattaaagat gttgtcagtt
     2941 aaataaatta ccaacatcca attttatagt attggtctgg acaggctttt caaatgttac
     3001 tattatggtt ttatcatcaa atgaatccag actttgtgat atgtaatttg taattccttc
     3061 taatacaaat ttatctcctt cttttctacc atgcaccacg aattttaatt tactatcttc
     3121 cataactaag tactatatat gaagtctatt ttgcttatat agtttatgtc attaaaaatg
     3181 aaacataaat tagaaaattg aaagaaaatt aaattaaaaa gatttgataa tttctataca
     3241 caaagataca gcaatattca attgattttt tttctctata tctatctagc aggtaacgag
     3301 ttagatttat caaagtgttt aattactctc ttttcacatt cttggccttc tggaggactc
     3361 aaatcatcac tagcaacaca agcatgatat ctatctccaa attgaatttt gcctatatct
     3421 gtgtctctat caaatgtaaa agttctttgt attgtactgc ctttgctttt atcaagttct
     3481 gcttgtacat cttctataac ttcagactgt atttgacctt ttatcatagc agcaattact
     3541 aatttttttg tatctttttt atctgccaaa ggagcaagta tttttatttt cactttgtca
     3601 atatttgagc cacttccagt attatttaca ttagctccat aaactgcttg agttttttca
     3661 tatacgtgtg agcccaattg gaacaaacga gcaggtgttg gtttacttgt gcttaaaata
     3721 tcgccttcat tacaatcaaa ttttgccaag gaattataat tattttctac atctcctact
     3781 tgatatgcac atacaaagaa ttcatctggt gtactagctt caactatgtc atttttttta
     3841 tttactttta attcaacatc tagtttattc tttgtcttat cgatagaaga tataggaata
     3901 tcttgtttga cctcttcacc atttataaaa cctataactc gtatgaattt ggtatttttc
     3961 atatctacat tttttaaatt aatattggct ttaactatga gcttgtcttt atcctctttc
     4021 tcctctttat catttttttg tgcaaatgct gatccataag aagaagatac gtataatatg
     4081 ctaacagcaa atgctattcc aataaaaata tatagacatt taatgtaacc cttttctacc
     4141 atcttatttg gtacaactat atggtatatt taatattttc gtaaaatatt ctataatgct
     4201 ataccgggta aaattaggaa tttgtttata ctgatatttc taatacttcc cttttgtcta
     4261 tagaattttt cttttataaa aagatgcttt tattgataga atcatttatt tagtaatgaa
     4321 ttctaacatt aaataatatt ctaatatcaa caatttttta tttatattct cctattatcc
     4381 atgttcaagc atggtcttga acttcaatta ctgggtgtta agggttaact ttaacaccca
     4441 tctatttatt ttaccaacaa tcaggtgatg ctaaaaagcc atacttggca caatattctg
     4501 ttgtgactga agattcacct gttagagtat catgaactag aataaaaaat aacatagcaa
     4561 taaaaaatag actgaaacca ctgaaagcaa attgtattat cgtttttatt ggttttagat
     4621 tcatgtgaaa tccatatgtt gtaatactat gtatttaaat tcatagtatg tttttcatat
     4681 tgtgctatga gaattttatc ggttaaacta tatcaataac ataaagaatt ttaagaagat
     4741 tgacaaataa tgcttaccaa aaaaaatttg ggagtttata cggctttatg tatagtgtaa
     4801 aggtaggtca cgttaccttt acatttaatt acatcctcga gagttatttt cttcaaattc
     4861 aatatcatag atttctatat ccttttcaga aatcatctta atccaatatc ttcttgttga
     4921 gaatcttatt gtctctgtta atctattcag atatttcata acttggtatt ttacaaattc
     4981 gacttttaat cctcctaatg cttggacatc ttttggagta taaggtaatt caatttctat
     5041 agaagaaaca tatacttcct tcggggtcat ctggtacaat ttttgggttt ccatggattt
     5101 tgattccttt aattgtttca atcccattcc acagaacttg tatgaaatta tacactaata
     5161 ttttacaatt atctttattt taaaataagt ttctaacaat aaaagctaaa tacattctta
     5221 aatttgatat aagaatatcc acagagtcaa tcgtctgtca tactaatagt ataaattatg
     5281 aaattggttt tttatatatc ataatttact ttgttaatat ataaaataat ttaaaacagt
     5341 attatgagaa acttttagag aattttatgt gttattctat atgagatttt attcacaaat
     5401 catataaata atctctagta tgctatttac atgaaatgtc ataatatcgc cgtctatcta
     5461 ataattccac ttgctctctt ctctattata tcattgtttg ataacgtaca agctcaaact
     5521 aactcatcat ctgccaatca aacgacatct caacaacaac agcaaaatca aaacgcaaat
     5581 ctcactgcgc agactttaat gaaaacagat atagttgcat taaagaatac tttgatgaac
     5641 gcacagttag caatagttga tggtaatatc aaacaagcat taaaagatgt tatggattta
     5701 gaaacacaat tggtattact aaaaccatct cctcctacta aatttataaa taatatacat
     5761 aaagctatag ctgctatatc tacatcagaa attgataaat cattgaatac tattacaaat
     5821 attcaagtta gtatattaaa agcagagaat ctaatattca aagctgcagt ggcaaatcct
     5881 caagtgatgc aacaaattca cactgtgagc tatgtagtac caactcctca atctgaaaag
     5941 atacaacaag ttgacacttc taaaaatgta
//
LOCUS       DASVEV010000192         4307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_859215, whole genome shotgun sequence.
ACCESSION   DASVEV010000192 DASVEV010000000
VERSION     DASVEV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859215"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VF222_08610"
     CDS             <1..1256
                     /locus_tag="VF222_08610"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7142526.1"
                     /translation="TTRSATKITVVERGIYSIINAAIKINYSNYKISIVTEDENDKQK
                     FHHLKNCEIVTVEKKYNTNAIKKSRALQFAVEYRRKTGENTNKFWIFHMDDESYVTNQ
                     TLLSLLKYIREDKGIASEGPIVYPLKFELANPITALAESVRPFGCYDCVSQMRNPPPL
                     HMHGSNLLVRSDAEDSIGWNFGSTLAEDQLFGYKLYERYGKKALGWHGGILLEQPPLN
                     IKDHFMQRRRWILGSLQNINKFPFWHRFKIAFKLSSYFMGFISGIASAILYVITFMSM
                     LPTSIQILVDQNISNPIIWAQYITSSFFPSSFPEITLKINEMASNNSIYPIDIGPVSI
                     LLIFPYIMWLLSYQIGLFLNLKHNNKYPLSKKIILHIQAVFLSLVLGILETFPAFYSI
                     IEYYWRNKMNAIKKIHNYDFYVINK"
     gene            1379..1852
                     /locus_tag="VF222_08615"
     CDS             1379..1852
                     /locus_tag="VF222_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142527.1"
                     /translation="MMLLITSYSGYQSATNFITKGKIIIGDTLVETEFPFKGTIKLVT
                     YLMVFSLIAWFTSIKIYEEAIRNLKVRIKTFIQIISIIAATITIYELFYNFIVWNSLI
                     TSNLFNNIFDPNSEIINYPMPETPWNLYFATQMLLAAVIITIHSYYVMTKSKKQS"
     gene            1923..2627
                     /locus_tag="VF222_08620"
     CDS             1923..2627
                     /locus_tag="VF222_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142528.1"
                     /translation="MNTCSYYTLEKANNKIKKMNKISLVWGPFLIISFLFTIFYFQND
                     IYAQLENIGNDDSIQVIKDPEKEVPSLVIIFGEKEVEMEPFMYSKINFTNKVKGSQSN
                     KSQDNIVTKIGSPTLYETRSDIDTQLTLKPGDNITLSYEKQPLTIKAYLIDYDTEDET
                     EIYPIKQIDISTFNIPNNSPDGLKSLEIRTFFDNNEQITYTMPVFVESSNPAITAENV
                     EDDNQEANNDKQNPKD"
     gene            complement(3355..3567)
                     /locus_tag="VF222_08625"
     CDS             complement(3355..3567)
                     /locus_tag="VF222_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142529.1"
                     /translation="MPRKLAIFIFWLVGFGIGFGGYLLFPNIINWFQTNMPQFLDASI
                     IGALIAGIIGSVLSTITIISWANRTS"
     gene            3818..4030
                     /locus_tag="VF222_08630"
     CDS             3818..4030
                     /locus_tag="VF222_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142530.1"
                     /translation="MERINAEMLAGIAFVCLALIFIFAATMNEVWAIILPADYLILAL
                     GFAFIALGVYTTRNNRKHSHEKSTHH"
     gene            4101..>4307
                     /locus_tag="VF222_08635"
     CDS             4101..>4307
                     /locus_tag="VF222_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7142531.1"
                     /translation="MIIYSFLIILAISFTNIIDNIINSVTNLIYNLGYFGIFIAAFIE
                     TIFPIIPSELIFPLAGYVVHSQGLG"
BASE COUNT         1661 a          580 c          592 g         1474 t
ORIGIN      
        1 ttactactag atctgcaact aaaataacgg ttgtagaaag aggtatttat tcgattataa
       61 atgcagcaat aaagattaat tattctaatt ataaaatttc aattgtaact gaagatgaaa
      121 acgataaaca aaaatttcat catttaaaga attgtgaaat agttacggta gagaaaaaat
      181 ataatacaaa tgctattaaa aaatccagag cattacaatt tgctgtagaa tacagaagga
      241 aaacaggaga aaatactaac aaattttgga tatttcacat ggatgacgag agttatgtta
      301 ctaatcaaac tttactatcg ttattaaaat atattagaga ggacaaagga attgcatcag
      361 agggtcctat tgtatatcca ttgaaatttg aattagcgaa tccaattacc gcactagcag
      421 aatcagttag accatttgga tgttatgatt gcgtatctca aatgaggaat cctcctcctc
      481 ttcatatgca tggaagtaat ttgctcgttc gatcagatgc cgaggattct attggatgga
      541 attttggctc aactttagca gaggatcagc tttttgggta caaactctat gagagatatg
      601 gaaaaaaagc actgggatgg catggaggta ttttattaga acaacctcct ctaaatatta
      661 aagatcattt catgcaaaga agaagatgga tcctaggatc attacagaat atcaacaaat
      721 ttcctttttg gcatcgattc aaaattgctt ttaagttaag tagttatttt atgggtttca
      781 tttcaggaat agcctctgca atattgtatg ttataacatt tatgtcgatg ctaccaacat
      841 ctattcagat actagtcgat caaaatatat ccaatccaat catttgggct cagtatataa
      901 cttcttcatt ttttccatca tcttttcctg aaattacatt aaaaataaat gaaatggcaa
      961 gtaataacag tatttatcct attgatatag gtcctgtttc tattctatta atattcccgt
     1021 atataatgtg gctattatct tatcagatag ggcttttcct aaatttgaaa cataataaca
     1081 aatatccttt atcaaaaaaa ataattttgc atattcaagc agtatttctt tcattagtat
     1141 taggaatttt agaaaccttt cctgcatttt attcgattat tgaatattat tggagaaata
     1201 agatgaatgc tattaagaaa attcataatt atgattttta tgtaataaac aaataacaag
     1261 atatagaaag ataactatag taatatcata ttagtaataa ttgaatttca taagagttaa
     1321 tcatagactt ataaaatcaa aaaaaaatac actaatccta ttctatttta gcgtatcaat
     1381 gatgttgcta ataacttcct atagcggata tcaaagcgca actaatttca taacaaaagg
     1441 aaaaataata ataggcgata ccttagtaga aaccgagttt ccatttaaag gtactattaa
     1501 actagtcacc tacttgatgg ttttttcctt gatagcttgg ttcacttcta ttaaaattta
     1561 tgaggaggca attagaaatc tcaaagttag gattaaaacg tttattcaaa taattagtat
     1621 aattgctgct acaataacaa tttacgaatt attttacaat tttatagtat ggaattccct
     1681 tattactagt aaccttttta ataatatttt tgatccgaat agtgaaataa taaattaccc
     1741 aatgcctgaa actccatgga atttgtactt tgcaactcaa atgcttttgg ctgcagtcat
     1801 aataactatt catagctatt atgttatgac caaatcaaaa aaacaatcct agaacaactt
     1861 acggtcggat aaaatattat attataatta catttataac tttataatat ataatgtttt
     1921 taatgaacac ttgtagctat tatacactgg aaaaggcaaa caataaaatt aaaaaaatga
     1981 ataaaatatc cctagtatgg ggcccgtttc taattatttc gtttttgttt actatttttt
     2041 attttcagaa tgatatatat gcacaattag agaatatagg aaatgatgac agtattcagg
     2101 taataaagga tccagaaaaa gaagtgccat cattagttat aatttttgga gaaaaagaag
     2161 ttgaaatgga gccttttatg tattctaaaa ttaactttac taataaagta aaaggatcac
     2221 aatcaaataa atcacaagac aatattgtga ccaaaattgg ctctcccaca ttatatgaaa
     2281 ctagaagtga tatagataca caactaactt taaaacctgg agataatata acattatctt
     2341 atgaaaaaca acctttgact attaaggcat acttaataga ttatgataca gaagatgaga
     2401 ctgagatata tccaattaaa caaatagaca tctcaacatt taatattcca aataattctc
     2461 cagatggatt aaaaagttta gaaataagga ccttttttga taataatgaa caaataacat
     2521 acacaatgcc cgtatttgta gaaagtagta atcctgcgat aacagcggag aatgtcgagg
     2581 atgataatca agaagcaaat aatgataagc aaaatccaaa agactaatta aattaactat
     2641 ctattgtttt atttcctaag attggttacc taagattggg catctttaca tttcatatca
     2701 gaatatgatc tcctaagcat gactattaaa tcacagatta aacgtaatag ttagaaaaaa
     2761 attattggta ggctagattt gttaagagga ttataaatta atattatttt tctttcaata
     2821 ttcaatacta ataaataaaa aatgttttaa agtaaacgat aaaaatgaaa catatgagaa
     2881 ccacctaatc aattcaaata aggaaacaaa acatatttct acatacagtt ttttttatta
     2941 tcacttttgg tattatcaaa aaatatatta aattaaattg caatgttaaa gaggctgaaa
     3001 atgataaaaa aagaaattca agaaattatg tgagattgtt aacataagat gtcctaaatt
     3061 tttgcttggt gtaagagaaa taaagatatc cgattatgga taacagacca ctaaagattg
     3121 taacgtttta gtttgaaaca taacaccaaa tttctattta ttcaaaaata aatttacatt
     3181 atcaaataag gaaatgtaaa taagaaatat tgaaatgata caagtaattg tataataaca
     3241 tgattaaata aacgtgtcta aaattcattt ataatagact aatatgcacg tatgccaaat
     3301 gcaattagaa gaaactattt tttgtatatt attttctata gaaatatttc taccttatga
     3361 tgttctattt gcccagctaa ttattgttat agtacttaat acagatccta taattccagc
     3421 gattaaagca cctatgatag aagcgtctaa aaattgcggc atatttgtct gaaaccaatt
     3481 aataatgttt ggaaacaata aataacctcc aaatccaatc ccaaaaccta ccaaccagaa
     3541 aataaatatt gctaatttcc ttggcaagtt attttctcct tattaactga ttccagttta
     3601 attgtataat agaaataaac tttaatgtgg aaattatcat atttaaaaat actttaaaaa
     3661 gatattaaga tagatggtta aatttgataa aactaaatgt ataaaattat atactagata
     3721 taaataaata aacactcatt tatttcgaag tataatatga aataattgag aaataaaaat
     3781 attaatattt ataatgtcaa gtatctattt gatttggatg gaaaggatta acgcagaaat
     3841 gcttgctggg atagcattcg tctgcttggc tcttattttt attttcgctg caactatgaa
     3901 cgaagtttgg gctattattt tacctgcaga ttacttgatt ttagctcttg gttttgcttt
     3961 tatagcttta ggagtttata caacacgaaa taaccgaaaa cattctcatg agaaaagcac
     4021 ccatcactaa ttttcgaact tagctcatta taccactaaa aagttttatt aagccaatta
     4081 atttttatct ttagttatat ttgattattt acagcttttt aataattcta gcgattagtt
     4141 ttactaatat catcgataac atcattaatt ctgttacaaa tttaatttac aaccttggtt
     4201 actttggtat ttttattgcg gcattcatag agaccatttt tccaataata ccaagtgagc
     4261 taatttttcc tttagctgga tatgttgttc atagtcaagg tttagga
//
LOCUS       DASVEV010000193        15577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1248987, whole genome shotgun sequence.
ACCESSION   DASVEV010000193 DASVEV010000000
VERSION     DASVEV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248987"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VF222_08640"
     CDS             <1..867
                     /locus_tag="VF222_08640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7142532.1"
                     /translation="ITLYESGHVLGGSTIVVENKGKRIFYTGDINTRGSKILRPADLD
                     IDEIDLMVIESTYSQTNQMPRSQSEKELLEFAYKIIDNGGTLFIPAFSVERAQEIACV
                     FKANKFPHKIVMDGMALKVNEIMLKYPEYIRDPIVFKKYIEEVEWIRDWKNRRRAIKE
                     PCVIISPAGMLVGGSAIFYMQNIMMSDKSGIALVSYQGENTPGRMLLEKGTFKLNGKI
                     KKCLAEVKQFEFSGHNSRDELFEILDNIKGNPKVLTVHGDGSSCIKFAEEIKEKYGFD
                     AIAPDSGDIFKI"
     gene            1146..2084
                     /locus_tag="VF222_08645"
     CDS             1146..2084
                     /locus_tag="VF222_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEX7142533.1"
                     /translation="MTVKYNKKNLVPLDPIKTINSGQVFLWEKYNNSWYGIDGNYVLK
                     ITIKDKELYEFYENRKHHFGLENTEIDYNSFPENKNWIYHFFRLCDNQEKINKTLSKD
                     QIIFNIYKKYKGLRLIRQDPFQCIISFVCASNTNIKRIKFMLKNISRKFGKKVTYNDI
                     DFNLFPNVNELSKASIQDLIGCGLGYRAKFVKSISNEIKCNLDLKSLKDMKYINAKSE
                     LTKLFGIGNKIADCVLLFSLEKTEAFPIDIWVYRSLCQYYGWMFEDSSMIKSKKISEN
                     EYKLVHEKIHNYFGEYSGYVQQYLYYHIRDSNKRKW"
     gene            complement(2122..2526)
                     /locus_tag="VF222_08650"
     CDS             complement(2122..2526)
                     /locus_tag="VF222_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142534.1"
                     /translation="MDHNPHNSTISLIIGILILSIFSLTNTYSQNSEMSVIVKLINAD
                     KYLAQPWDINGQLRDKYTDFEISKDQTTFKVSENQTIQLIIPLSNSTEDLNRYSIFVD
                     ASSTIDDIDVFGTIDSLKKEDNQITLSLANAR"
     gene            3067..3552
                     /locus_tag="VF222_08655"
     CDS             3067..3552
                     /locus_tag="VF222_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142535.1"
                     /translation="MFEKKARRNWKCEYEDTVYKIYDWDGNLTGYFYPLYNIQRKSEM
                     EEEDIIVRMNENKEKVQGGHLMVPFLKLDLLDIDYLNIDEAITRLQNSIQRALRWKKW
                     IEDNKKKFNLIQSLVSTSREDRNLLAVLFEIYKEIVLDEKALVKEITPLLDQLHKDEL
                     L"
     gene            complement(3933..4451)
                     /locus_tag="VF222_08660"
     CDS             complement(3933..4451)
                     /locus_tag="VF222_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX7142536.1"
                     /translation="MRIGVISDSHDDIINVKQAIKIFNEYEVDYVIHAGDYIFPGIVL
                     EFKNLHKNIKFVGVFGNNDGEKIGLVKSFITIAGDLKGDFGELKLDDEILGIYHGTNE
                     DIKSSLIRANYYDVLICGHTHKKDNKKINRTQILNPGSSHRNLQNDELNKKASVIIYY
                     TGIKKYEFIELR"
     gene            4832..5527
                     /locus_tag="VF222_08665"
     CDS             4832..5527
                     /locus_tag="VF222_08665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7142537.1"
                     /translation="MKKLFLIFDLGQTLINEWNFIEFFDQKLLELVNGFGGRIDSKNY
                     ITLRNNIIKNRLIGNGGIEELIITLCRLILPIGYDKIILKKIKPDLDHGKRKFFCLND
                     GAKKVVETLSKYHEIGIISNQPEEALEPLMNANILSLFRVLIPSKKKMKEPYWKLPLD
                     VIKLTGFPASRCIMIGDRLDIDIFPANQLGMKTIRFTDSLFNLQSAEIKYEIPTYTVK
                     KLEEIPNIIKDIV"
     gene            complement(5539..6636)
                     /locus_tag="VF222_08670"
     CDS             complement(5539..6636)
                     /locus_tag="VF222_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HEX7142538.1"
                     /translation="MINSAQVVDIVKNYDKKSINIGILGSHSALEVMDGAVDECIRTV
                     CICQKGRETPYLRFKRLSDTVIVLDRFIDIINPEYQKKLRDLNVLMIAHRAFSVYIGY
                     DNIENNFLIPLFGNRYLLRAEERTAPKNQYYLLNKSRIKHPELIKPNDISGPSIVKIQ
                     EAKRKLERAFFFVNSYKDYVEKTEIKIKSGLISRTDLESAIIEEYVIGTYFNFNFFYS
                     PLQKDLEFLGIERRLQTNLNDFTTSLTAKQQLELDIELQNIEIGHTPASIRESMLEKV
                     FDLGDKFVNTVKEEYPPGLIGPFSLQSIITKDLDIFVYDVSFRVPGNPILSTTSPYTK
                     YYYGTTFGVGRRIAMEIKEAISNDRIKEIIT"
     gene            complement(6695..7018)
                     /locus_tag="VF222_08675"
     CDS             complement(6695..7018)
                     /locus_tag="VF222_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7142539.1"
                     /translation="MITLDNLLMQMPFGFEWIFIIIIIVVLFFGVKKIPELAKSFGKA
                     TAEFQKAKIEAKRDLDKYKKENTDIEREKLESIAETLRIDSTDKNNEDLKKAIEKEIN
                     KNKNI"
     gene            7173..7490
                     /locus_tag="VF222_08680"
     CDS             7173..7490
                     /locus_tag="VF222_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142540.1"
                     /translation="MQYHKAAFLGQERSRKAQMKLFEYTGFSMLTYTIKKGSSGFEPV
                     GEEMLVGNLNNGREMILFICDKDGYAKAQSKSMTIEEGNKLVEKMVLDGFIKFDGEIK
                     TVI"
     gene            complement(7577..8296)
                     /gene="pyrH"
                     /locus_tag="VF222_08685"
     CDS             complement(7577..8296)
                     /gene="pyrH"
                     /locus_tag="VF222_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7142541.1"
                     /translation="MVEYPQKKRIVIKLSGSIFSLSDNHDQDSNNYYNIFKQYSDVLI
                     NLTPNVQLIVITGGGSIARLYIKFARKLGLDEASLDLLGIAISRVNAKLLIASLGNYA
                     YPEIPQSLDDVGRFIESNKIIVSGGLHPGQSTNATSALIAEKTRASEFINATDVKGIY
                     NSDPRKNNNAQLFEKIELGKLLTMLLKESSMAGEYDLLDIVSLKVIERSKIRTKVILS
                     DPKNISNAIQGRNYIGTEVVV"
     gene            complement(8309..9550)
                     /locus_tag="VF222_08690"
     CDS             complement(8309..9550)
                     /locus_tag="VF222_08690"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7142542.1"
                     /translation="MLLEFTGEITDPIHKYIRITDLEKKLIDTEVFQRLRKIRQLAGA
                     HLVYPSAQHTRFEHSIGAMHLAGLAAETLLDKGYITYKEDVESLRIAALLHDIGHGPF
                     SHLFEEVLRENESNNLTHEIIGKKIIKESIIIDILGKYGYDTDYVCKLSFGESQKMFY
                     DEIIAGSLSADIMDYLPRDSFFTGAEYGKVDYHRLISSLEVLSNDHLGINKSAINSLE
                     SMLISRYQMFKAVYFHKTVRAGEVMLLHSLMSADKQLNFSNFCIEEYLDYTDEKTIDI
                     ICSLKENKFASDLALDYKKRKLFKCVYEKFLQNKHNAFDKELAGKQIRDFLRGIREYL
                     DVDESFIFIDISQAPSMPLTPSKEELNSIMLVDKDLEFETPTSAIPLIDSITGFLDMI
                     RIYTPERNREKLEKVLRNIDL"
     gene            complement(9810..10130)
                     /locus_tag="VF222_08695"
     CDS             complement(9810..10130)
                     /locus_tag="VF222_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142543.1"
                     /translation="MSIQEFLLPDENIRFHSKISVKYGEKKYNVLITDMRIILYAQRG
                     HILKTDDVISERLDKLREIKYCEKGLVFRSAKISIQSSINLDIVGSCTELKQLFNNLQ
                     VIVY"
     gene            10342..11160
                     /gene="tatC"
                     /locus_tag="VF222_08700"
     CDS             10342..11160
                     /gene="tatC"
                     /locus_tag="VF222_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7142544.1"
                     /translation="MPTEMSLRDHLSEMRSRILRVSIIILIIIIFCITFGFKVYPINI
                     SITNVPNTIYLPFIDPNPFNNIAIQVTSFMRESLLPVGVELIQTAPGQAFFAQIYVAA
                     LIGIIISVPLLIREMYEFILPAIEEKKKKRINLVKMLIPTIGLFIIGIIFSYLLVIPF
                     TLDFLYKYGESMGVETFLNINDFITFVLQFLIAFGISFELPLLMYIISLSQLIDHNFW
                     RKNFRYSIIIIVVYGAIITPDGSGITMWLIAGPMILLYLIGIFLVQAKIAEKKV"
     gene            11244..13937
                     /locus_tag="VF222_08705"
     CDS             11244..13937
                     /locus_tag="VF222_08705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HEX7142545.1"
                     /translation="MTQDYFIKDENKLSNEISDLPENKNVILLSAIYSGEDQKVLLKF
                     YDPDLEVIYFWKDRTNHKPYCYTKIEYKEKAEEIVKKESKYNILQKKKMDLISDKEID
                     VIKIIAPDPLSIGGTDNSIREKITAWEADIKYHENYLYDNALIPGTYYYRKNDSIYQY
                     KYEIDPQIRSKLDELIDNNHQDLLDNKTTNYNYYISQWVNLLNQPIPNIKRIAIDIEV
                     ESEEGRIPHPRDHDKTVTAIGLAGNDGFRKVYVLNKGSNEKNSLIPEAEIVESEKELI
                     LAAFRDIKNYPIILTFNGDDFDLPYLYMRSQDVRIDPNKEIIKKEDIPFIVKRDTFIK
                     RGMQAEPVNLKHGIHIDLFRTFQNRSVQIYAFNHKYAEYTLNAISEALLKDHKIEFEG
                     DISAIPMHKLAEYCLKDADLTLRLSTFNDNLLIKLLIVISRISHLPIDDVSRFGVNQW
                     IRGMLYFEHRQSNSLIPRRDELLAKGVSSTTAIIKEKKYRGGLVVEPKVGIHFNVVVV
                     DFASLYPSIIKVHNLSYETVNCSHQSCKNDPEQQIPETTHWICKRRRGLTSLLIGSLR
                     DLRVRYYKQLSKDKTTPIDNRQLYEVISQAIKVILNASYGVMGAEIFPLYCLPVADAT
                     AAIGRVTTTRTIEKCKQIGIEVIYGDTDSLFLKNPSSEKVKEISEWARSQLEVDLEID
                     KTYRYVVFSNLKKNYLGVLGDGTVDVKGLTGKKSHTPPFIRKAFYKILEILKEVNSEN
                     DFQKAKEKIKKIIQSYANDLEAGKIHPSELSFNVMVNKSPDRYGEKTNNSKIKSIDGR
                     NIDFITYKGLPQHIKAAMLLHQSGREIKAGDIISYVKTKTNDGVKPVDLLKNTNEIDT
                     EKYLEAMESTFDQLLSALDYNFKAILGKDRQANLDELFWSK"
     gene            14003..14974
                     /locus_tag="VF222_08710"
     CDS             14003..14974
                     /locus_tag="VF222_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX7142546.1"
                     /translation="MNALETLEPFFVTPEKTNAKCSFGDFPTKISFQDSDAVLYGVPL
                     DITTTFGKGTSYGPEAIRITSAKQIESFLLEENQEIYDKIKIYDIGDLEIVNSKRNEK
                     YKKILETKLKRISKINEINSLIRKEKKIPILLGGEHTLSYFSIKSIARENPIIIHFDA
                     HRDMKPIYDRKKICHTTPFFHLIDEGYIKGNNIIQIGIRQSDKEENDIATNNGVNTYT
                     GLDINKDIHRITREIKKLTNDRNIYITFDIDVYDICYVPCTGTPEPFGLNPFQVLQIL
                     KSINKSAILIGLDMVEVAVKNRDYREGTLATQTLYRIFSNLTLKPNL"
     gene            complement(15186..>15577)
                     /locus_tag="VF222_08715"
     rRNA            complement(15186..>15577)
                     /locus_tag="VF222_08715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
BASE COUNT         5823 a         2197 c         2275 g         5282 t
ORIGIN      
        1 ataacactct atgagtctgg acatgtttta ggaggctcaa caattgttgt tgaaaataaa
       61 ggaaaaagaa tattttatac tggggatatt aacacaagag ggtctaagat tttgagacct
      121 gcagatttag atattgatga aatagacctg atggtaattg aaagtactta ttcacaaact
      181 aaccaaatgc cgagatcaca gtcagaaaaa gagttattag aatttgcata taaaataatt
      241 gataacggcg gaacgttatt tattccagct ttctcagtag aaagagctca ggagatagca
      301 tgtgttttca aagccaataa atttcctcat aaaatcgtta tggatggaat ggcacttaaa
      361 gtaaatgaaa ttatgttaaa atatcctgaa tatataagag atcccatagt tttcaaaaaa
      421 tacatagaag aagtagaatg gattagagat tggaaaaata gacgaagagc gataaaagaa
      481 ccttgcgtaa ttatttctcc tgcaggaatg ttagtgggag gatctgctat tttttatatg
      541 caaaatatca tgatgtcgga taagagtggt attgctcttg tctcttatca aggagaaaac
      601 acgccgggaa gaatgttact tgaaaaaggc acttttaaat taaacgggaa aataaaaaaa
      661 tgtctagcag aagtaaaaca atttgaattt tcaggtcaca acagtcgaga tgaattattt
      721 gaaatattag ataatataaa aggaaatcca aaagtactga cggtccatgg agatgggagt
      781 tcttgtataa aatttgcaga ggaaataaag gagaaatatg gatttgatgc gattgccccg
      841 gattctggtg atatttttaa aatttaaatg aaaaaatagt aaatttaaaa ttaattgaat
      901 ataataattc aaacattgat aataaaaaat acagatccat ttaaacttaa aaaatgtaca
      961 tcttgtaaaa aagatattat attaaacgta aaatatttta cttatccact atcacttcaa
     1021 tatttttgta ttggatgtgc tcaaaaagaa attcccaaga taatagatac actacaaaac
     1081 gatttaaaaa aaataaataa ttagaatttt aacatatttt cttataactt agaatgagat
     1141 ataaaatgac tgttaaatat aacaaaaaga atcttgtccc tttggatcct ataaaaacaa
     1201 ttaacagtgg acaagttttt ctatgggaga aatataacaa ttcctggtat ggaatagatg
     1261 gaaattatgt tttaaaaata actatcaaag ataaagaatt atacgagttc tatgaaaata
     1321 gaaaacacca ttttggatta gagaatacgg agatagatta taattctttt ccagaaaata
     1381 aaaattggat ttatcatttt tttaggttat gcgataacca ggaaaaaata aacaaaacac
     1441 tatccaaaga tcaaattatt ttcaacattt acaagaaata caaaggtcta agactaatac
     1501 gacaagatcc ttttcaatgt attatttctt ttgtttgtgc gagtaacact aatataaaaa
     1561 gaataaaatt tatgctcaaa aatatctcta gaaaatttgg taaaaaagta acatacaatg
     1621 atatagattt taacttattt cccaatgtaa atgaattgag taaagcgtct attcaggact
     1681 tgatcggatg tggtttggga tatagagcta aatttgtcaa atcaatatca aacgaaatca
     1741 aatgtaatct tgatctaaag tcattaaaag atatgaagta tattaatgca aaatcagaat
     1801 taactaaact ttttggaata ggtaataaaa ttgcggattg tgttctttta ttttctttag
     1861 aaaaaacaga agcatttcca atagatattt gggtatatag atctttatgt caatattacg
     1921 gatggatgtt tgaagatagc agtatgataa aaagtaaaaa aatatcagaa aatgagtaca
     1981 aattagttca tgaaaagatt cataattatt ttggggaata ttcaggttac gtccaacaat
     2041 atctatatta tcatatcagg gattctaata aaagaaagtg gtaaactgta ggaaataaaa
     2101 ataaaaaata atgaatttcc gttaacgggc attagcaaga cttaatgtaa tttgattatc
     2161 ttctttcttc agagaatcta tggtaccaaa aacatcgata tcatctattg tagatgaggc
     2221 gtctacaaaa atagaatacc tattcagatc ttctgtagaa ttagataggg gaattattaa
     2281 ttgaatagtt tggttttcac taactttaaa ggtagtttga tctttggaga tttcaaagtc
     2341 tgtatactta tctctcaatt ggccattaat atcccaaggt tgtgctaagt atttatctgc
     2401 attaataagt ttaactatta cactcatttc agaattttgg gaataggtat tagttaaaga
     2461 aaaaattgat aaaattaaga tacctattat taatgaaatt gtagaattat gaggattatg
     2521 atccataaat ataataaaat aaacatctat ttatataatt cactcaaaat taagattctc
     2581 ttttgtaaat tcttacaata tttatgattg tatataaaat actaatttga tgttattgga
     2641 tgattaatat aaattttaat taattagtat ttcattagtt tttcttcata atcagacaac
     2701 ttcagacatt atatacatct tctgactaga ttccataaaa ccctatgatt tgctatctac
     2761 aaatcaagat ttttttctta ttgatgaact atctgtatct gtcagaaata cacagtagag
     2821 agaaatttta atatgaatcg gtttaagtcg atttatctaa tactgtttca aattataatt
     2881 tgtatgatat cttaaaatac tcgttgtatt aattttatag gatttctttt gatttgggta
     2941 caatttccaa ataatgttct aatctagtgt ttgttattca acttccttta tgtcagatac
     3001 gagaaataaa aaattacgtt atagtagata aattttttta ttgtaataca aatattaata
     3061 catcaattgt ttgaaaaaaa agctagaaga aattggaaat gtgaatatga ggacaccgtc
     3121 tacaaaattt atgattggga cggtaatctc actgggtatt tctatccact atataatata
     3181 caaagaaaaa gtgaaatgga agaggaagat attattgtga gaatgaatga aaataaggaa
     3241 aaagtccaag gtggtcattt aatggttcca tttttaaaat tggacctttt agacattgat
     3301 tatttgaata tagatgaagc tattactcgc ttacaaaaca gtattcaaag ggctctcaga
     3361 tggaaaaaat ggattgaaga taataaaaaa aaatttaact taattcagag cttagtctct
     3421 acatccagag aagatagaaa ccttttggct gtactttttg agatatataa agaaatagta
     3481 ttggatgaga aggcacttgt aaaagaaatt acacctctcc ttgatcagtt acataaggat
     3541 gaattattat aattcttctt taaaataata aaataatgtt ttgcaaaatc ttaaatacat
     3601 ttattataat ggtaaagagt aaggtaaagt ataaacaaga acaaattatt ctttaggcga
     3661 ttgaattacg caaaaatttt gtaaaatcag aaatcattgc cggatataca tatgttctct
     3721 acttgcaaaa ttgaattcga aatccaatgc ctacatagag tctaaaaata aatatttatt
     3781 tccaaaatat aaaaattaaa cttaaataaa gaaacggctg acttaaaaat aggatttagg
     3841 ttatatgtca tattattaat aaaataaaaa ctatcgataa ttgtatttag actcgaagga
     3901 tgaaaatttc ttctgatatt tcctagcttt atttatctaa gttctataaa ctcatatttt
     3961 tttattccag tataataaat tataacagac gcttttttgt ttaattcatc attttgtaaa
     4021 tttctatggg agctaccagg attaagtatt tgtgtcctat taattttctt attatctttt
     4081 ttatgtgtgt gaccacatat caaaacatca taataattag cacgaattaa tgaacttttt
     4141 atatcttcat ttgttccatg atatattccc aatatttcat catctaattt aagctctccg
     4201 aaatctccct taagatcccc tgctattgtt ataaaacttt taacaagacc aattttttct
     4261 ccatcattat ttccaaagac accgacaaac tttatatttt tatgaagatt tttaaattca
     4321 agaacaatac caggaaatat ataatctcct gcatgaatta catagtctac ttcatattca
     4381 ttaaaaattt ttattgcttg tttcacatta attatatcgt catgggaatc tgatattact
     4441 ccaattctca ttataataaa aaactatact atgttttatt tttgttttta gttatatgta
     4501 tagaattatt atccatccaa gagaaaattc aaatttgaat cgaaccacat atttaacaat
     4561 ttcaaaatta tgatttttta atattatgta tttataatta ataataaaat ggagtattct
     4621 tatacattat aatatattaa ttctaaatag tctaccgatt acgaaaaatg aatgtattaa
     4681 tccattatta gcgttaaagt tagtgcctta aaattttaat attaggcaaa aacattctag
     4741 aagattgtca agtatttaaa ttatttgaaa ttttagaatt aggctttcga atctaaacta
     4801 taaataaata aaaatcttaa attaagattt gatgaaaaaa ctatttttga tatttgattt
     4861 gggacaaaca ttgataaatg aatggaactt tatcgaattt tttgatcaaa agctcttaga
     4921 attagttaat ggatttggtg gaagaataga ttctaaaaat tacattacgt taagaaataa
     4981 tattataaaa aatagactga ttggaaatgg tggaatagaa gaattaatca tcactctttg
     5041 cagactcatt cttcctatcg gttatgataa aatcatttta aaaaaaatta aacctgattt
     5101 agatcatgga aaaagaaaat tcttttgtct taatgatggt gcaaaaaaag ttgtcgaaac
     5161 actgtcaaaa tatcacgaga tcggtataat ttctaatcaa ccggaagaag cactagaacc
     5221 tttaatgaat gcaaatatac tttcgctctt tagagtgttg attccgtcaa aaaaaaaaat
     5281 gaaagaacct tattggaagt tacctcttga cgtaatcaag ttgacaggat ttcccgctag
     5341 tcgatgtata atgatcggag ataggctgga tattgatatt ttccctgcaa atcaattagg
     5401 aatgaaaaca ataagattta ctgattctct ttttaatttg caatccgcgg aaattaaata
     5461 tgaaattccc acatatactg tgaagaagtt agaagagatt cctaatatta ttaaagatat
     5521 cgtatgagaa ttttaaatct atgttattat ctctttaatt ctatcgttgg aaatagcttc
     5581 ttttatttcc atagcaattc ttcttcccac tccaaatgta gtaccataat aatattttgt
     5641 ataaggactg gtagtagata aaattggatt tccaggaaca cgaaatgata catcgtatac
     5701 aaatatatct aaatctttag taataatact ttgaagagaa aaaggaccta taagtcctgg
     5761 gggatattct tctttaacag tatttacaaa tttatctccc aaatcaaata ctttttccaa
     5821 cattgattca cgaatgctag caggtgtgtg tcctatctct atattttgta attcaatatc
     5881 taattctaat tgttgttttg ctgtaagaga agttgtgaaa tcattaagat ttgtctgtaa
     5941 tcttcgttct atacctaaaa attcaagatc cttttgtaga ggagagtaaa agaaattaaa
     6001 attaaaataa gtaccaatta catattcttc gattattgca gattctaaat ctgttctact
     6061 aattaatcca ctttttatct ttatttcggt tttttcaaca taatctttgt atgaattaac
     6121 aaagaaaaaa gcacgttcca gcttcctttt tgcttcctga attttgacaa tcgatggtcc
     6181 tgatatatca ttaggtttta ttaattctgg atgtttaatt ctagatttat tcaacaagta
     6241 atactgattt ttaggtgctg tacgctcctc tgctcttaac agatatctat tcccaaaaag
     6301 aggtattaaa aagttatttt ctatattgtc atatccaatg tatacagaaa aggctctatg
     6361 agctatcatt aaaacattca gatctcttaa cttcttttga tattcggggt ttatgatatc
     6421 aatgaatcta tctagaacta ttacagtatc agataacctt ttaaatctca aataaggcgt
     6481 ttctctccct ttttggcaga tacaaacagt tctaatacat tcatctacag caccatccat
     6541 cacctcaagt gctgaatgac tacccaaaat accaatgttg atacttttct tatcataatt
     6601 tttaacaata tcaacaactt gagctgaatt aatcattatt aatgaaattt taatagtata
     6661 ataaattgtt taaggagaaa ttaaatttgg catattatat atttttgttt ttattgattt
     6721 ccttctcaat agctttcttt aaatcttcgt tatttttatc agtagagtct attcttaaag
     6781 tttctgcaat agattcaagt ttttcccgtt caatatcagt attttctttt ttatatttat
     6841 ccaaatccct tttagcttcg atcttggctt tttgaaattc tgctgtagcc ttaccaaaag
     6901 attttgctaa ttcaggaatt ttttttactc cgaagaataa tacaactata ataattataa
     6961 taaatatcca ttcaaaacca aaaggcatct gcatcaaaag attatctaaa gtaatcaaac
     7021 ttctttcaaa atattgttaa tatcaataac aatttaagtg ttaaatcaat cgaattacac
     7081 tttttcaata gatatgcatt tagtgaaaaa gtataatttc taaaaaacta gatagacatt
     7141 aataattata aaccaataca aaagtatccc taatgcaata tcataaagct gcgttccttg
     7201 gacaagaaag gtcaagaaaa gctcagatga aactgtttga gtatacagga ttttccatgt
     7261 taacatatac tattaaaaaa ggaagttccg gatttgaacc agtaggagaa gaaatgctag
     7321 ttggaaattt gaacaacggt agggaaatga ttttatttat ctgtgataaa gacggctatg
     7381 cgaaagcgca atctaaatcc atgacaatcg aagaaggtaa taagttagta gaaaaaatgg
     7441 ttcttgatgg ttttataaaa tttgatggag aaatcaaaac agtaatctga attcattttg
     7501 atgattaacc ttgtataata tcaacaagat tgaaatttgg gtatagagtt taacatgatt
     7561 tttttttcat ttacacttac accactactt ctgttcctat atagtttctc ccttgtatag
     7621 cattagatat atttttcgga tcagagagaa ttacttttgt tctaatttta gacctttcta
     7681 ttacttttaa agatacgata tccaataaat cgtattcacc agccattgaa ctttctttta
     7741 atagcatagt tagtagttta cctaattcaa ttttttcaaa taattgtgca ttattatttt
     7801 tcctcggatc agaattatag attcctttga catcagttgc attgataaat tcacttgctc
     7861 ttgtcttttc tgcaattaga gctgaggtag cattagtact ctgtccagga tgtaatcctc
     7921 ctgaaacaat tattttatta ctttcaataa acctacctac atcatctagt gactgaggaa
     7981 tttcaggata agcataatta cccaaagaag cgataagcaa ttttgcattt actcttgata
     8041 tggctatacc tagcaaatct aggcttgctt catctagtcc taattttctt gcgaatttga
     8101 tataaagtct tgctatactt ccacccccag taataactat taattgtaca ttaggtgtga
     8161 gattaattaa aacatcacta tactgtttga agatattgta atagttatta ctgtcttggt
     8221 catggttatc agaaagacta aaaatacttc cgcttaattt tattactatt cgtttttttt
     8281 gtggatactc aaccatttct atcacctttt aaaggtcaat gttcctcaac actttttcaa
     8341 gcttttctct atttcgttca ggtgtataaa tacgaatcat atccaaaaaa cctgtaattg
     8401 aatcaattaa tggaatagcg gaagtaggcg tttcgaattc taaatcttta tcaactaaca
     8461 ttattgaatt caattcttcc ttggaaggag taagtggcat agaaggagct tgagagatat
     8521 caataaatat gaaactttca tcaacatcca aatattctct tattcctctc aaaaaatctc
     8581 taatttgttt ccctgctaac tctttatcaa aggcattatg tttgttttgt aaaaattttt
     8641 cataaacaca tttgaatagt tttctttttt tataatccaa agccaaatct gaagcaaatt
     8701 tattttcctt caaagaacaa ataatatcta tagttttttc atcggtatag tctaaatatt
     8761 cttcaataca aaaatttgaa aaattgagct gtttatctgc actcataaga gaatgcaata
     8821 acattacttc acctgctcta accgttttat gaaaatacac agctttaaac atttgatatc
     8881 tagagattaa catggattca agagaattaa ttgcagattt attgatacct aagtgatcat
     8941 ttgaaagaac ttcaagggaa ctaattaacc tgtgataatc aacttttcca tattctgctc
     9001 cagtaaagaa actatctcgg ggcaaataat ccataatatc tgctgataaa ctacctgcta
     9061 tgatctcatc ataaaacatt ttttgagact ctccaaaaga aagcttacag acatagtcag
     9121 tatcatatcc atattttccc aaaatatcaa taattattga ttctttgatg attttttttc
     9181 cgattatctc atgagtgagg ttatttgact cattctccct tagaacttct tcaaaaagat
     9241 gagaaaaagg tccatgtcct atatcatgta ataaggcagc tatccttaaa ctttcaacat
     9301 cttctttgta agtaatgtac cctttgtcaa gaagagtctc tgctgctagt cctgccaaat
     9361 gcatagcacc tatggaatgc tcgaaacggg tatgctgagc actcgggtat actaggtgag
     9421 cacctgccaa ctgtcttatt tttcttaatc tttgaaaaac ttcagtatct attaattttt
     9481 tttctaaatc agtaatacga atatacttat gtataggatc ggttatttct cctgtaaatt
     9541 ctaaaagcat ttaagtaaat tattaaaaat tcattttctt ataaatggtg ttggtatgac
     9601 aaaaacaaat aaacctattc catggttaat tcgaaaataa atgatattta attaattcat
     9661 ggaaagtaaa tgtatcgaaa ggtgaatgac tcaaaaataa gataaatatt ttagtagata
     9721 gaaaatctaa ttgtgcttgt tttaaataca ggaattattt tcctttttga agatacaaat
     9781 ccaaataatg aaataaattt ataatagatt caatatacta tgacctgtaa attattaaat
     9841 aattgtttta attctgtgca agatccaact atatcgagat ttatactaga ttgaattgag
     9901 atttttgcgc ttctgaatac taatcccttt tcgcaatatt ttatttcacg taacttgtct
     9961 aaccgttcgc taataacatc atcagttttt aaaatatgtc ctctttgtgc ataaagtatt
    10021 attctcatat ctgtaatcaa aacgttatat tttttttcac cgtatttcac acttattttg
    10081 ctatggaatc taatattttc atcagggaga agaaattctt gaatcgacat atttgatatc
    10141 cacaaatgat atgattagga ttatttatca actatagtca taactaatta tccaaattga
    10201 taatttaatt aaataataca tttacaacaa aaataaaatt atcaaataat atcaacgaga
    10261 ttgtctcttt tattaatata tagtacgcat ctaatttaaa atagatatca aatatatttc
    10321 actaagtaga tctttaaaca aatgcccaca gaaatgtctt taagagatca tttgtctgaa
    10381 atgagatcta ggatattaag ggtttctatt attatcttaa taattattat attttgtatt
    10441 acatttggat tcaaggttta tccaattaat ataagcataa cgaatgttcc aaatactatt
    10501 tatcttccat ttattgatcc aaatcctttt aataatatag ctatacaagt tacctctttt
    10561 atgagagagt ctttacttcc agtaggagtt gaacttattc aaactgctcc tggtcaggct
    10621 ttctttgcac aaatatatgt tgccgcgcta ataggaatta ttatttccgt gccgctacta
    10681 attagagaaa tgtatgaatt tattctacct gcaatagaag aaaagaaaaa gaaaagaatc
    10741 aacttagtta aaatgttaat tcctaccata ggattattta ttataggcat aatattttca
    10801 tatttacttg taattccctt tacactcgat ttcttataca aatatgggga atcaatgggt
    10861 gtagagacat ttttaaatat taatgatttt ataacttttg tcctacaatt tctaattgct
    10921 ttcggaattt catttgaact tccactgcta atgtatatta tatccttatc acagttaata
    10981 gatcataact tttggagaaa aaatttcagg tattcaataa ttataattgt tgtttatgga
    11041 gctattatta ctcctgatgg cagtggtata acaatgtggt taattgctgg accaatgatt
    11101 ttattatatt taataggtat ttttcttgtt caagctaaaa ttgccgaaaa aaaggtttaa
    11161 tattttttaa tactacatcc ttgttggtac tattatttga aaagattaaa ttttaataga
    11221 atgtaaataa ggtagaagag atagtgacgc aagattattt tattaaagat gaaaataaat
    11281 taagtaacga aatatcagac ttaccagaaa ataaaaatgt tatattgtta tcagctatct
    11341 attctggtga agatcaaaag gttttattaa aattttatga tcccgacttg gaagtaatat
    11401 atttctggaa agatagaact aatcataaac catattgtta tacgaaaata gaatataaag
    11461 aaaaagcaga agaaattgta aaaaaagagt caaagtataa catactacag aaaaaaaaaa
    11521 tggatttaat ttctgataag gaaattgatg taataaaaat tattgccccc gatcctttat
    11581 caataggagg aactgataat agtataagag aaaaaataac cgcatgggaa gctgatatta
    11641 aatatcatga aaattatcta tacgataatg cacttattcc tggtacatat tattatagaa
    11701 aaaatgacag tatttatcaa tataaatatg aaatagatcc ccaaatacgt agtaaactcg
    11761 atgaattaat cgataacaat catcaagatt tattagacaa taaaacaacg aactacaact
    11821 attacatctc ccagtgggtt aatctgttaa atcaaccaat tccgaatatt aaaagaattg
    11881 caatagatat tgaggtagaa tcagaagaag gacgaattcc acatccaagg gatcatgaca
    11941 aaacagttac tgctatagga ttagcaggta atgatggttt cagaaaagta tacgtcttaa
    12001 ataaaggatc gaatgaaaaa aattctttga ttccagaagc agaaatagtt gaatctgaaa
    12061 aggagttaat cctagcggca tttagagata ttaaaaatta tcctatcatt cttaccttca
    12121 atggagatga ctttgatctt ccgtatttat atatgaggtc tcaggacgtg agaatagatc
    12181 caaacaaaga aatcataaaa aaagaagata tcccatttat tgtaaaaagg gatactttca
    12241 taaaaagagg gatgcaggca gaacctgtaa atctcaaaca tggaattcat attgatttat
    12301 ttaggacctt ccaaaataga tctgtacaga tttatgcctt taaccacaaa tacgcagaat
    12361 acacattaaa tgctatttcc gaagctttac ttaaagatca taaaatagaa tttgaaggag
    12421 atatttcagc tattcctatg cacaaattgg cagagtattg tttaaaagac gctgatctca
    12481 cattaagact ttccacgttt aacgataacc ttttaataaa attattgata gtaatttctc
    12541 gaatatctca tttaccaatt gatgatgttt caagatttgg agtaaatcaa tggattaggg
    12601 gaatgttata ttttgaacac agacaaagta attctcttat acctagaaga gatgagctct
    12661 tagctaaggg agtatcatct actactgcaa taatcaaaga aaaaaaatat cgtggtggtc
    12721 ttgtagtaga accaaaagta ggaatacatt ttaatgttgt agttgtcgat tttgcatcac
    12781 tttatcctag tattataaaa gttcataatc tttcatatga gactgtaaat tgttctcatc
    12841 aatcctgcaa aaatgatcca gaacagcaaa ttcctgaaac tactcattgg atatgtaaaa
    12901 gaagaagagg tttgacatct cttctcattg gctctctacg agatttaaga gtaagatatt
    12961 ataaacaact ttcaaaagat aaaacaaccc ctatagataa tcggcagctt tatgaagtaa
    13021 ttagtcaggc tataaaggta atattaaatg ctagttatgg tgttatggga gcagaaatat
    13081 ttcctctcta ctgtctgcca gtagccgatg ctactgcagc cataggaaga gtaacaacaa
    13141 cacgaacaat agaaaaatgt aaacaaattg gtattgaagt aatttatggt gatacagact
    13201 ctttattttt aaaaaatccc tcttctgaga aagtaaaaga aatttcagaa tgggcaagaa
    13261 gtcaattaga ggtcgactta gaaatagaca aaacatacag atatgtggtt tttagtaatt
    13321 taaaaaagaa ttatcttggg gtacttgggg acggaacggt agatgtaaaa ggattaaccg
    13381 gtaagaaatc acatacacct ccgttcataa gaaaagcatt ttacaaaatt ctagaaatac
    13441 ttaaagaagt aaattcagaa aatgattttc aaaaagcgaa agaaaaaatc aaaaaaataa
    13501 tacagagtta tgctaatgat ttagaggctg gtaaaattca tccttcagaa ttgagtttta
    13561 atgttatggt taacaaatca cctgatcgat atggagaaaa gacaaataat tccaagataa
    13621 aatcaataga tggaagaaat attgatttca taacatacaa aggattacct caacatataa
    13681 aggctgcaat gttacttcat caatcaggaa gagagatcaa agcaggagac ataatttcat
    13741 atgttaaaac caaaactaat gacggtgtaa aacctgttga tctgcttaaa aatacaaatg
    13801 agatcgacac cgaaaaatat cttgaagcta tggaatcaac tttcgatcaa ctattatcag
    13861 cattagatta taacttcaaa gccatattag gaaaagatag acaagctaat ttagatgaat
    13921 tattttggtc caaatgataa gaaaaatact gataatgaga atctagaaac aatttaattt
    13981 gtaacttcat aaagcaaggg aattgaatgc tttagaaacc ttggagccct tttttgttac
    14041 tcctgaaaaa actaatgcaa aatgtagttt tggagatttt ccaacaaaaa tatcatttca
    14101 agattcggat gcagttttat atggagttcc tttggacatt actactactt ttggtaaagg
    14161 tacatcttat ggtcctgaag caattagaat aacatctgca aaacagatag aatccttttt
    14221 gttagaggaa aatcaagaaa tttatgataa aatcaaaata tatgatatag gtgaccttga
    14281 aatagtcaat tctaaaagaa atgagaaata taaaaaaatt ttagaaacta aattaaaaag
    14341 aatctccaaa ataaatgaaa taaattcatt aataagaaaa gaaaaaaaaa tcccgatttt
    14401 attaggagga gaacacacat tatcatattt tagtataaaa tcaattgccc gggagaatcc
    14461 cataataatt cattttgatg cacaccgaga tatgaaacca atttatgaca gaaagaaaat
    14521 ttgtcatact acaccatttt ttcaccttat tgatgaaggc tatattaaag gtaataatat
    14581 cattcaaatt ggaattcgtc aaagtgacaa ggaagaaaat gatattgcta ctaataatgg
    14641 tgttaatacc tatacaggat tggatattaa taaagacatt catagaatca caagagaaat
    14701 aaagaaacta acaaatgata gaaacatata cattacattt gatatcgatg tatacgatat
    14761 ttgttatgta ccatgtacag ggacacctga accatttgga ttaaatcctt tccaggtatt
    14821 acaaatacta aaatctataa ataaaagtgc tatactgata ggacttgata tggtagaagt
    14881 tgcagttaag aatagagatt atcgagaagg aactttagca actcaaacac tttacagaat
    14941 tttttcaaat ttaacattaa aaccaaattt atagtgaata gtgacaagta taataaagaa
    15001 agaaagaaac taaaaaatag atgtttaata ttcagataca tagaaaatta aattatttaa
    15061 taaataaaaa aaagttagaa aattaaatta tttaataaat aaaaaaaagt ctcatcgtgt
    15121 ataggatcga attatccagt agtactctga acctttacgt atactaacca aggttaaaga
    15181 tactaatatc aggtgccttg ggttgttggt actggcaggc tgaacatctc gccgaagctt
    15241 gatgcgtaca cctccagccc atcaatgcta tcttctatag ctacccttct ccttacggaa
    15301 ggctgtcttt tctcgagaat ggcttcctcc ttagatgctt tcaggagtta tccaaaacag
    15361 cttagctgct cagcgtgccc tgtcggacag ctgataaacc agaggctgcg ttgccctgtt
    15421 cctctcgtac tagggacaac ttcccctcag acaaccacgc ccctgtcagg cagagaccga
    15481 cctgtctcac gacggtctaa acccagctca cgttcccctt taatgggcga gcagccccac
    15541 ccttggctcc tgctgcagaa ccaggatggg aagagcc
//
LOCUS       DASVEV010000194         7009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_860333, whole genome shotgun sequence.
ACCESSION   DASVEV010000194 DASVEV010000000
VERSION     DASVEV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860333"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VF222_08720"
     CDS             complement(<1..1152)
                     /locus_tag="VF222_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7142547.1"
                     /translation="MINTVETTPELVSAVYKKSRKNISYYKEKIKNEGLTLSEKILIG
                     HLFDTEKDDQNKNLERGKSYVLLRPDRVALQDVTGQMTLLQFMQSRLKRTILPTTVHC
                     DHLIQARLEGVSDTKAALYENNEVFKFLESASRKYGVGFWKPGAGIIHQVVLENYAFP
                     GGLMIGTDSHTPNAGGLGMIAVGVGGLDAAEAMAGMPWEILYPKRIGVYLTGKLNGWT
                     SPKDIILYVAAKLTVSGGTNAIIEYFGPGSKSISCTGKATITNMGAEIGATSSVFEYD
                     KRMETYLRSTNRADIAEVANENMDILTQDPEVDANPEKYFDQVIGIDLSTLEPYIVGP
                     HTPDLGRPISQMKQDVLRNNYIDKISVALIGSCTNSSYEDMSRASGIAEQ"
     gene            complement(1672..1797)
                     /locus_tag="VF222_08725"
     CDS             complement(1672..1797)
                     /locus_tag="VF222_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142548.1"
                     /translation="MVENKKAEDAKKIKDNKDIEDDEELKEELKELEVESTKAGD"
     gene            complement(1939..2808)
                     /locus_tag="VF222_08730"
     CDS             complement(1939..2808)
                     /locus_tag="VF222_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7142549.1"
                     /translation="MKQNLRLSSRSVSFKWFFAEKPVIYILLFTYLFITLVPLVWVLS
                     TSFKSNEEATSLPPKFIPTNPTLDNYIFVITEPHIVHSLFNSFIVSIGSTVISVAISA
                     LGGYAFARFQFKGKNILLSVILGLFMIPVLINIIPLYIMFSQIGLLNSLFGLILTFQI
                     LIIPLNIFLLKSYFETIPRELEEAALIDGCSSLGVLRRITIPLSMPGFAIAAVLAFRF
                     SWNEFILPIVLANKPDSMVFQVALYEFVSLYSIEWGYLTAGINIAIIPILVLMLVFQK
                     QMIRGLTLGAIRH"
     gene            complement(2831..3625)
                     /locus_tag="VF222_08735"
     CDS             complement(2831..3625)
                     /locus_tag="VF222_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7142550.1"
                     /translation="MIWNIYISLHDVSILTIIKDWDFIGFENFNNIFSDPNFYNSFKV
                     TLVFVCGSVLLQFGIGLLIALLLNQQIRASGFFRTLFIIPWTVSTVITGFSFKFLFDD
                     SFGIINYGIEKIGLESIGWFSDPNLAIWIVIVANTWYGAPFTILFLSAGLLSINPTLY
                     EAAIVDGATKFRRFFYITLPLLKSFILTNLILVTVWTINFFDLQLIMTNGGPLSSTTT
                     ISLYMYKQAFEFGYFSEGAAVGILLIIMNIAVAFVYLKLFDARNKK"
     gene            complement(3754..5067)
                     /locus_tag="VF222_08740"
     CDS             complement(3754..5067)
                     /locus_tag="VF222_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7142551.1"
                     /translation="MNKLKVLITVSFAIVSLFFISQINLITVIAQEEITLTAIFAEPK
                     ERWDMLVENATKILNDRHPDKTIKIDYRVLPYSDTRTQILTAMAGKTPIDLISLDQIW
                     LGEFAEGGFLSDLTYNTQIWNKSSDWYIQNWKGGLYNDQVKGIWAWTDVRALWYWKDL
                     LDKAEINPEQLETWNGYISASKKLNQFLQKDRIQAMHLVGANHSPDMWYPYLWMIGGE
                     ILEKRDGHPTKQNYWYPIYNSDLGIKALHFLKDQVEAGIKPQINHFWGQEFGDKKFAV
                     MLEGSWLLGQFAKNEWNTLEEKIGMLPMFPVPNISNTNTSTLMGGWLLSIPETSENKE
                     LAWELITIMLEPDVLIPMLLQHGYLPTQKSIGEGQYSEKLESTIPYYEELISLISLGK
                     GRPNIPEYPIVAEHIRNAIEQVYNGMDPKQALDEASKQSAKALGW"
     gene            5354..6397
                     /locus_tag="VF222_08745"
     CDS             5354..6397
                     /locus_tag="VF222_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7142552.1"
                     /translation="MQIDSGKFLVLLGPSGCGKTTIIKSIAGLIDPTEGEIYIDEILI
                     NKLPPKDRDVAMVFQSYALYPHMSVYDNIAFPLKMRKLSKNEIKNKVENTASLLELTQ
                     FLKRKPKELSGGQMQRVALARALVRKPKLFLMDEPLSNLDAKLRMQMRTEIKKLQKKI
                     GITTIYITHDQIEAMSMADQIIVMNKGVIQQIGSPHQIYYQPTNLFVANFIGSPPMNF
                     LNFKISQTSEDFNLYSSQIVIKISKINIPLLETISSEIVLGIRPKDIMIYDKNHKNES
                     DTLSFKGRLLFTELLGDEVIYEFELEGNNSIVKVSSNTDNFHYKQNDDFNLIVKYKDI
                     HFFDSITEERLLF"
     gene            6768..>7009
                     /locus_tag="VF222_08750"
     CDS             6768..>7009
                     /locus_tag="VF222_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142553.1"
                     /translation="MDNVNIHTCRVCGSKVTDLVSLKVENDNIIKLGKFFNGNWVCDD
                     CFLKQRGIESESIDYSTEEKDKSKDIASSRDVSPNTP"
BASE COUNT         2498 a         1096 c          997 g         2418 t
ORIGIN      
        1 ttgttctgca atacctgatg ctcttgacat atcctcatat gacgagtttg tacaactacc
       61 aatgagagcc actgaaatct tatctatata gttatttctt aatacatctt gtttcatctg
      121 ggaaattggt ctacctaaat caggggtatg aggaccaact atgtagggtt ctagagtgga
      181 taaatcaatt ccaattactt gatcaaaata tttttcaggg ttagcatcaa cttctgggtc
      241 ctgtgttaaa atatccatat tttcgttagc aacctctgca atatctgctc gatttgtaga
      301 tcgaagatat gtctccattc ttttatcgta ttcaaaaact gaacttgtcg ctccaatctc
      361 tgcgcccatg ttagttatag tagcttttcc agtacaacta attgatttgg aaccgggacc
      421 aaaatattct attattgcat ttgtcccacc agaaacagtt aatttagctg ctacatacaa
      481 aatgatatct ttcggagatg tccatccatt taactttcct gtaaggtaaa cccctattcg
      541 ttttggatat agaatttccc atggcatacc tgccatagct tccgcagcat ctaaaccacc
      601 aacccctact gctatcatcc ccaatccacc tgcatttgga gtatgtgaat cagtccctat
      661 cattaaacca ccaggaaatg cataattttc caagactact tggtgaatta ttccagcacc
      721 gggtttccaa aatcctacac cgtattttct tgatgcagat tctaagaatt taaaaacttc
      781 attgttctca tataatgcag ccttagtatc agaaactcct tctaatcttg cttgaattaa
      841 atgatcacaa tgaacagtag taggtaaaat agttcgcttt aaacgtgact gcataaactg
      901 taaaagagtc atttgccctg tcacatcttg taatgcaact ctatcaggac gtaataatac
      961 ataacttttt cccctttcta aatttttatt ttgatcatct ttctcagtat caaaaaggtg
     1021 acctatcaat attttttcac tcaatgtaag accttcattc ttgatttttt ctttatagta
     1081 tgaaatattt ttcctagatt ttttataaac agcagaaacc aattcaggtg tggtttctac
     1141 tgtatttatc atagtcatgt tttttacatt atttattatg ttatacagat ataagaaatt
     1201 tcttgatcat tctgttagta gttggaatat caattaggag agtttataga acgatacagt
     1261 aaaatttgac taaatatatt tttaactata tttttctaat tccttacaaa tatacatggt
     1321 atcttatcgt gtaagaagta gtaatttatt aacaaatttt atgcatgaat attttcaatt
     1381 ttatcacaag aaagtaaaga atgaaaaaaa atatcataaa ttgaagttta ctattaaact
     1441 tataaaaggg aatactaatt tctctaggat cagggttaaa agtatgtata gtagataatt
     1501 tctacttttt acttagtgta ttaattcttt attttgttac atagcgtgta tatgtatctg
     1561 tttataaaat gtaattaaaa ttgttaggta caacatataa tacaaagata aatcagctca
     1621 aattaacagt taccaaataa ttgttattgt ttgagtggtt gttaagatat tttaatctcc
     1681 tgcttttgta gattctactt ccaactcctt taactcttcc ttgagttcct cgtcatcttc
     1741 aatgtctttg ttatctttta tttttttagc atcttctgct tttttatttt ctaccatacg
     1801 tttactcttg tgtatataga ataaattatt tactattaaa attcaattag actaaataat
     1861 atttctataa tacattatta ttattattat tattattatt attttaatag tactttttat
     1921 tctaattaaa taaaaaaatt aatgtctaat agcacctagg gtgagtcctc tgatcatttg
     1981 tttttgaaaa actaacatta atactaaaat gggaattatt gctatattta ttccagcagt
     2041 taaataaccc cattcaatac tatataacga aacaaattca tataatgcca cttgaaaaac
     2101 cattgaatct ggtttattcg ccaaaactat tggaagaata aattcattcc atgagaatct
     2161 aaatgctagg acagcagcaa tagcaaaccc gggcattgac aaggggatgg ttattcttct
     2221 taatactcct aaacttgaac atccatctat taatgccgcc tcttctagct ctcgaggaat
     2281 agtttcaaaa tagcttttta gcaaaaatat atttaaagga ataattaaaa tttgaaaagt
     2341 gagtattaga ccaaaaagtg aatttaatag acctatttga gagaacataa tatagagtgg
     2401 aataatgtta attaaaactg ggatcataaa aagtcctaaa ataacactaa gcaaaatatt
     2461 tttaccttta aattgaaatc tagcaaaggc ataacctcct aaagcactga ttgcaacact
     2521 aataacagta cttcctatgc tcacaataaa gctattaaaa aggctatgta cgatatgtgg
     2581 ttctgtaatt acaaaaatat aattatcaag agtaggattt gtgggaataa attttggtgg
     2641 aagggaagtt gcttcttcat tagatttgaa agaagttgaa agaacccaca caagtggtac
     2701 caaagtaata aaaagatatg taaataacag tatgtaaata actggttttt cagcaaagaa
     2761 ccatttaaaa gatacagatc tggatgacaa gcgcaaattt tgtttcataa tgtattcatc
     2821 ttacaaatta ttatttctta tttcgcgcat cgaataattt aaggtataca aacgcaactg
     2881 ctatattcat gatgattagt aatatgccaa ctgctgctcc ttcagagaaa tatccaaatt
     2941 caaaagcctg tttatacatg tataacgata ttgttgttgt agaagacaat ggaccgccat
     3001 tggtcataat caattgcaaa tcaaaaaagt taattgtcca aactgttact aatatcaaat
     3061 tggttaaaat gaaagatttt aaaagtggaa gagtaatata aaaaaatctc ctaaatttcg
     3121 ttgctccatc aacaatagct gcttcataaa gtgtaggatt aattgatagt aatcctgcac
     3181 taagaaataa tattgtgaat ggagcaccgt accacgtatt tgccacaata actatccata
     3241 tggctaaatt tggatctgaa aaccagccga tagattcgag acctattttt tctattccgt
     3301 aattaataat tccaaaacta tcatcaaaga gaaacttaaa agagaaacct gtaataactg
     3361 ttgatactgt ccatggaata ataaacaatg tcctaaaaaa gcctgaagcc ctaatttgtt
     3421 gatttaacag aagggctatt aagagaccaa tcccaaattg aagcaagaca gacccacata
     3481 caaaaactaa tgtaactttg aacgaattgt agaaattggg atcagaaaag atattattaa
     3541 agttctcaaa tcctataaaa tcccaatctt ttattatagt aagaatggac acatcatgta
     3601 atgaaatata tatattccaa atcaatggaa taaaaagaaa tacaaataat attataagac
     3661 taggtaatag aaaaagataa ggctcaaaac tttttaagat taacattgga gtatgttata
     3721 tcgacaagtt aaaaaaataa taaatcttaa attttaccaa cccaacgctt ttgcggattg
     3781 ttttgaagcc tcatctaagg cctgttttgg gtccatgcca ttgtatactt gttcaattgc
     3841 atttcttata tgctctgcaa ctataggata ttcaggaata tttggccttc cttttcctaa
     3901 tgaaattaga gaaatgagtt cttcataata aggaattgtt gattccaact tctcagagta
     3961 ttgtccttct cctatgctct tttgagttgg aaggtagcca tgttgtaaca acatcggaat
     4021 caaaacatca ggctctaaca ttatagtaat aagttcccat gctaattctt tattttctga
     4081 tgtttcaggt atacttaaaa gccatcctcc cattaaagta gaagtatttg tatttgaaat
     4141 atttgggaca ggaaacattg gaagcattcc tattttttcc tctaatgtat tccattcatt
     4201 cttagcaaat tgtcccaata accaagatcc ttcaagcatt acagcaaatt tcttatctcc
     4261 gaattcttga ccccagaaat gattgatttg aggtttaatt ccagcctcaa cttgatcttt
     4321 taaaaagtga agcgctttaa tacctaaatc gctgttatag atgggatacc aataattttg
     4381 tttggtagga tgtccatccc tcttttcgag aatttcccct cctatcatcc ataggtaagg
     4441 ataccacata tcgggtgagt ggttagcacc tactaaatgc attgcttgaa tcctgtcttt
     4501 ttgtaaaaac tggtttaatt ttttagatgc tgatatatat ccattccaag tctctagctg
     4561 ttcaggattt atttctgctt tatccaacag atccttccaa taccatagtg ctctcacatc
     4621 tgtccatgcc cagatccctt ttacttgatc attatacaat ccacccttcc agttttgtat
     4681 ataccaatca gacgacttgt tccatatttg tgtattatac gtaagatcgg ataaaaatcc
     4741 tccttctgcg aattcaccca accatatctg gtctaatgaa ataagatcaa taggggtttt
     4801 tcctgccatt gcagtaagga tttgtgttcg tgtgtcagaa tatggtaata ctctataatc
     4861 aattttgatc gttttatcgg gatgtctatc atttaatatc tttgttgcat tctccacaag
     4921 catatcccat cgctccttag gttcagcaaa aattgcagta agagttattt cctcttgagc
     4981 aataacggta ataagattta tttgtgaaat aaaaaataaa gatacaattg caaaagacac
     5041 tgttattaaa acttttaact tgttcatttt agaattataa cagaagttac aactactact
     5101 attattaatt atttactaat caatattata tttttttaga ttacctatgt aataaagcaa
     5161 tactgtatac gattctattt agaataaatg cattttccat caacctaatg taatttattt
     5221 caattctttt attgttacga aataatatta aatattactt tttagtaaac ttagtgagta
     5281 ttgtctgtca agaataacaa ttaaaaatct tagtaaaagt tatgggaatg taaatgcagt
     5341 tacaaaattt tcagtgcaaa ttgattcagg aaaattttta gtacttttgg gaccatctgg
     5401 atgtgggaaa actactataa taaaatctat agctgggctt atcgatccaa cagaaggaga
     5461 aatttacatt gatgaaatct taataaataa acttccaccg aaagatcgtg atgtggcgat
     5521 ggtttttcaa agttatgcat tatatccaca catgagtgta tatgataata tcgcatttcc
     5581 attaaaaatg agaaaattat ctaaaaacga aataaaaaat aaagtagaga ataccgctag
     5641 tctgttggaa cttactcaat ttcttaaacg aaagccaaaa gaattgagtg gaggtcaaat
     5701 gcaaagagtg gctttagcaa gagcacttgt tagaaaacct aaattatttc taatggatga
     5761 accactcagt aatttagatg ctaaattacg tatgcaaatg agaactgaga ttaagaaatt
     5821 gcaaaagaaa ataggaatca caaccatcta tatcactcac gaccaaattg aagctatgag
     5881 tatggccgat caaattattg ttatgaacaa aggagtaatt caacagatag gttcacctca
     5941 ccagatttat taccaaccta caaacctttt tgttgcaaat tttataggaa gtccaccaat
     6001 gaatttttta aattttaaaa tatctcaaac atcagaagat tttaatcttt attcttctca
     6061 aattgttatt aaaatttcaa agataaatat accgctatta gaaactatat cttcagaaat
     6121 tgttttagga atacgaccta aggatattat gatttatgat aaaaatcaca aaaatgaatc
     6181 tgatacctta tcttttaaag gacgactatt atttacggaa ctacttgggg atgaggtaat
     6241 ttatgaattt gaattagaag gtaataatag tatagtcaaa gtttcatcaa atactgataa
     6301 ttttcactat aaacaaaatg atgattttaa tttgatagta aaatataaag atatacattt
     6361 ttttgattct attacggaag aacgattatt attttaatga ttgatatata taacgaattt
     6421 tactaattta taataataat tttagattct accaagattt agttacataa taaaacagct
     6481 caacgaagtt taactttcta tcagtaatca ttaccactaa gttttaaatt ttaaaattta
     6541 agaaaaataa taattaataa aaaaatcaag attatttttt taataaggta aattttaaag
     6601 gagtaaatat tttagaaact agggagttag ggaactaata aaaatctcgc aatctctaac
     6661 aaataaatat gaaaaaagta taaaagtatt agaaaagttg taattattaa attttatcta
     6721 attaggaatg atctaatcct taaaaacttg tttgatcaat aactattatg gataatgtta
     6781 atattcatac ttgtcgagtg tgtggctcaa aagttacaga cttagtctct cttaaagttg
     6841 aaaatgataa tataattaaa ttaggaaaat tctttaatgg aaattgggta tgtgatgatt
     6901 gttttctaaa acaaagagga atagaatcag aatctataga ctattcaaca gaagaaaagg
     6961 ataaatctaa agatattgca tcatctagag atgtatctcc taatactcc
//
LOCUS       DASVEV010000195        16089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1443660, whole genome shotgun sequence.
ACCESSION   DASVEV010000195 DASVEV010000000
VERSION     DASVEV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443660"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF222_08755"
     CDS             <1..310
                     /locus_tag="VF222_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142554.1"
                     /translation="KILKECFEISDNYSQIRTKIQIDSKLQKTILEKLYDEFKKDSTE
                     INTLDGIKIIIDETSWILFRISNTEHVLRISIESTTNKINTLYRNVNERIIKIHDQIK
                     "
     gene            294..1349
                     /gene="thiL"
                     /locus_tag="VF222_08760"
     CDS             294..1349
                     /gene="thiL"
                     /locus_tag="VF222_08760"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7142555.1"
                     /translation="MTKLNEKEIIDLFYSNLLEKNKIKFQIRDDVSVLSFKDLKKNKI
                     RSFNSSNIIIKCDMLVESTDVVKQMKIWQIARKSIVSAVSDMSAKGIKPPYFSLLALG
                     IPSSWTKIKIKNLILGFKKASKEFGIVFLGGDTNESKELVIDCTLVGFLDSTSNTIPL
                     RNNAKAGDLVITSGEFGYTSAGLKILLFKSKAIPSFKKQAISSIILPKPNQKFGTLLG
                     KYFSSSIDSSDGLGISLYELAKQSRVNFYVDDIPIPRGLIEFAKSNSLDIYDLIFNGG
                     EEYEIIATINPSNLQKVSLLAKKFHLPMFVIGKAITGSGNVFVKSDNKLELFHGRKIE
                     KSKDYFLLENKGFLHFS"
     gene            1396..1803
                     /locus_tag="VF222_08765"
     CDS             1396..1803
                     /locus_tag="VF222_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7142556.1"
                     /translation="MSETEDITQYRWGVAHIFSSYNNTLVHITDISGAETISFSSGGR
                     HVTADRYESSPYAAMKSAVSAADAAKSKGINALHIRVRAVGGVGPRIPGPGAQAAIRA
                     LARAGFRIGRIDDVTPVPHDTTRKPGGRRGRRV"
     gene            complement(1984..2400)
                     /locus_tag="VF222_08770"
     CDS             complement(1984..2400)
                     /locus_tag="VF222_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7142557.1"
                     /translation="MGEDPDIEILKAKKMQEIMQRMMKQKKENIQKDIKKREPSEREI
                     LLSYLYDRGDEVLNLAESQFPSETRAIINRIVELINSGEINRKISGGELLSLFRTIGM
                     NVKINTTIKIEEHGKYMSFAEKLKQNKEIDDEENKR"
     gene            complement(2401..3873)
                     /locus_tag="VF222_08775"
     CDS             complement(2401..3873)
                     /locus_tag="VF222_08775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7142558.1"
                     /translation="MNKIITKRGNHVIEKLNEFEFKINVDDSKKMNVPVTIYADDSLI
                     LKMSEDRTLDQAVNVSTLPGVRKHVVVLPDGHEGYGFPVGGVAATDMEEGVISPGGVG
                     YDINCGVRLLRSNLNVNQVKPHLKELVNELFNSIPLGVGSDGAIKLTRSELDELLIEG
                     VDWAIEHGYGYKQDSEVCEEKGRMIGANPESVSDTARKRGAPQLGSLGSGNHFLEIQQ
                     IQEIFDENAAKVMGLEKGQITVLIHCGSRGFGHQVCSDYLRYSEKSLKKYNIELIDRE
                     LACVPNNSIEGENYRKAMYSALNFAWSNRQIITHFTRKTFEKIFSSSETGLDMKLVYD
                     VAHNIAKVERHKVDGEGMRDLVIHRKGATRAFPAGMEQVPEKYRQIGQPVIIPGSMGT
                     ASWILVGNKKSLDLSFGSTAHGAGRTMSRGAAKKSYTVEKVKAELDAKGIYIKALTKN
                     GIVEETPLAYKDVDAVVNVSDKLGIATKVAKLIPIGVIKG"
     gene            4780..5403
                     /locus_tag="VF222_08780"
     CDS             4780..5403
                     /locus_tag="VF222_08780"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7142559.1"
                     /translation="MFTGIIESLCEIKSINEMKKGNSKYPAEIKFSIDIGNLSKGLKI
                     GQSIAVNGVCLTITKLNNNIAEFSVIGETVRKTCLDSIKKGDRVNIERSMRASDRFDG
                     HFVQGHVDCTGTIHDKIVFPTETKLIIKISEELKESFQYIVRKGSITIDGISLTVVDI
                     DNKNLLSISLIPHTLKKTSLGIKSIGDKVNIEFDIIGKYISKLLPKI"
     gene            5458..6138
                     /gene="ribB"
                     /locus_tag="VF222_08785"
     CDS             5458..6138
                     /gene="ribB"
                     /locus_tag="VF222_08785"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7142560.1"
                     /translation="MSTVKDAIAALSQGKFVLIHDDDSRENETDMIIAAENIKPYHIA
                     TIRNDAGGLVCLAIDGYISTKLGLSYIHDILNCVSNDHPLFTTMTNGRSPYGDKPSFS
                     ISVNHRDTYTGITDYDRALTISKLASVCKNIDNGGVSEFIKCFRTPGHVSLLIAAKGL
                     LKERFGHTELAVYLAKIAGLTPAVAICEMLDSHTFRSLSIDEANKYAKLKDIPLLEAD
                     QLKTYSVI"
     gene            6146..6610
                     /gene="ribH"
                     /locus_tag="VF222_08790"
     CDS             6146..6610
                     /gene="ribH"
                     /locus_tag="VF222_08790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7142561.1"
                     /translation="MVKVHLAIVVSEFNHEITDLMLEEAKVHASKLNSNVSHICYVPG
                     TFDMPLIISHLLQNNTIDAVITLGAVIKGDTNHDIIIAENTARLISDLSLQYKKPIAL
                     GISGPNMTIEQAWERAKIVPIRAVNAAVNMVTRIKKLNESQYSQNKTTIIIK"
     gene            6607..7383
                     /locus_tag="VF222_08795"
     CDS             6607..7383
                     /locus_tag="VF222_08795"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HEX7142562.1"
                     /translation="MNIQITIIKIEGYGPWTLVLGSDREAKLQMLQAQIYHDLQYLFS
                     KYNCLVFFNRFDEYFVITNTLSLEDHVSVIKEISIKHPNLCISFTIGNGKTPLDANIQ
                     AYQSRKNEKFLVKKFQVYGEINNNLSVNSSLNDKKVKILHIDINDSSGFGNHLSPYET
                     TIKVMKSYVLLMDKFLEKNSLTFFLGGDNFMIIAADLIEKDEISNLLNSISDELDIKF
                     NCGIGFGRTSRESAKNATEALDSIRKLRKKGEIIPIYEIK"
     gene            7380..8690
                     /locus_tag="VF222_08800"
     CDS             7380..8690
                     /locus_tag="VF222_08800"
                     /inference="COORDINATES: protein motif:HMM:NF005552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7142563.1"
                     /translation="MTLFIRNISLLYGKDLVYINKGFICINKNGIITEAGSEDTYFDK
                     YKFTDTKVYDGTGFLVIPGFINSHTHIADSIGKDISFSSTFNEMIHPVYGIKKKILNK
                     STKEHLKYFIRTSAISMMIKGTISFVDFREGGPEGIELLKGSIGDLPIKSVILGRVEQ
                     YFDLKKILEIKKQTKENSDEKLVDKLQKLSLESLSRVLELSDGLGLSGANENTDDALK
                     QYIHKVKEFNKKNNYVYHRKKIIAIHAAESKSTIKFSKLLTGKTEVERIINFLKPDIL
                     IHMTHATFEDLTLALHNNVGIVLCPRSNGILGNGIPDIVTMVKLGCKISIGTDNIMLN
                     SPDLLQELDYLWKVIKSHSSKYSNSIDPKELLKMITVNPGEIFDLNSGSIDSGKYADL
                     LFIDKYHIDLQPIHNIYSSIIHRLSYGAIKAIMINGRFINETPY"
     gene            8674..9342
                     /locus_tag="VF222_08805"
     CDS             8674..9342
                     /locus_tag="VF222_08805"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HEX7142564.1"
                     /translation="MKLPTDLKVIINSAMTLDGKISSYTGNSRISSKKDLVRVHKLRS
                     KVDAIVVGINTVLIDNPMLTVRFIRNNRIYPARIIIDSHGRIPLDSKILKTAATIKTI
                     IVVTKQASKDTIEEIKKLGAHVIIIGSKLINLKKLFNMLYKMGYRKILIEGGGELNWS
                     CLHDGIVNELIITIAPIVIGGRNAITLVEGKGYSTISQGIKLKLTKIIQNKNDNEITL
                     YYNS"
     gene            complement(9350..9868)
                     /locus_tag="VF222_08810"
     CDS             complement(9350..9868)
                     /locus_tag="VF222_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HEX7142565.1"
                     /translation="MPSIDNEESFAKISNLIGGEEYQKVAKALLNTEDATDEEIAGAT
                     GMRINVIRKVLYDMFGKALITGIRVKDEKKGWFVYRWRAKSDQIDNFIDNQKKKILDR
                     LQKRLEYEESSEFYHCGNNECQRIKFDIAIESFFKCPTCKENLAMVDNNYTKEALRYK
                     IDQILSEISTEK"
     gene            complement(10019..11290)
                     /locus_tag="VF222_08815"
     CDS             complement(10019..11290)
                     /locus_tag="VF222_08815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7142566.1"
                     /translation="MTGLFDELGLNKQLLLALKESGYTTPFPIQEKAIPIILKGNDVI
                     GQAHTGTGKTAAYSLPILMKMKNARNAQALVLVPTRELAIQVAKEMNKLAKYTETKVA
                     AIYGGQNMRNQIIRLERGIHIIVATPGRLIDHIGQKTIYLDTIDFVVLDEADRMLDMG
                     FIEDINYILSKVKHIKQLCLFSATILPEIIEISKRYMKNPIHITVNQDDITLKTIEQM
                     YLITNEKEKYNHLCDLIKQSNNNQMLIFTSTKQKAEELAMNLRKKELLKVTSLHGDLS
                     QRERNYAMHKFRDEQEQILVATDIAARGIDIPSIKYVINYDIPRDPITYFHRIGRTAR
                     ANSDGKAISLVAIEKISDFEKILRFTKYKVRQLNEEIGIPIPKIQKEFKKHNYLNKNK
                     PFNKWSKHNYKNSKKKNYNYKKSSNRNFRKH"
     gene            11478..12380
                     /locus_tag="VF222_08820"
     CDS             11478..12380
                     /locus_tag="VF222_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142567.1"
                     /translation="MAEDEELREKKLRGSGGYVIARISDYEQKKGNLGGPELFIAGIG
                     RLPKDRILKYYCNKCEKDYPTSPDLNYENPNEDVGEGVVLIEKGEYKCSVCNAVLSQY
                     RKFNNETPPPPPSKTKSFSQQSNSSDVTKSVNDISDSSPKNKSESENSILTSNNTHEE
                     NISDSIQSESTDTKSSNVTIAKGKYFPIQSIIGMPVYDHEAMLVGNVQEIGLRKTLNG
                     TIQITLKIDNRDKISETNGDELYNEITWSDVSKIGDIVLINWEQKKISSSNTSPRDKK
                     ICVACKYHNESDAMYCEQCGKKLE"
     gene            complement(12405..14549)
                     /locus_tag="VF222_08825"
     CDS             complement(12405..14549)
                     /locus_tag="VF222_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7142568.1"
                     /translation="MTSSSSSFDSKNIFFNPQSIAVIGASEKPGVGKAIFSNIINGYK
                     GKIYPITPSNPTVFGINAFKSVLDISEDIDLAVIATPNKTVPQVMKEIGKKNIKGAII
                     VSAGFKEVDESGSQLEKQIGDIGKQYGIRIIGPNCLGIMSLSQSSMMNATFLKITPDV
                     GGIALVSQSGAICAATVEDAIDQGIGFSKVISMGNKVDMDENDILELISEDDDTRVIV
                     MYLEDIHDGKRFMQIAKKITKEKKKPILVLKSGRTPEGAKAAMSHTGALMGSDETYDA
                     LFEQSGVIRVDTMQELFETSTAFAKQPIPPSNGGVVIVSNAGGPAIISTDACSKYGLK
                     MADISQSESEIAKVIPPHGSARNPVDIVGDADFNRFEKVLDEVLSNNNVSAVVTMCTP
                     SATLDYNELAKTIIKTSKDSGKTMLAALMGLAEGIENKKILSEGGIPHFMYAEPAIRT
                     LEAMFRHRRWIEKEDIPPKKFEADKTKARDVLENVIKSNRTNLLEEEGYEILKAYGFS
                     TPKKFLATNEEESARAAEEIGYPVVMKIVSPQIIHKSDAGGVKIGIKNQQEAIKAFNE
                     ILLNVKKYKSDAEIKGILVQEMIGSAKETILGAKRDPLFGPLIMFGLGGIYVEVLKDV
                     IFRIAPIDEQQAKEMVESIKTIKLLKGARGEKESDIEGIVESIQRLSQLVTDFPEIEE
                     FDMNPLLVLEKGNGVRAVDVRIGLKNQKDKTI"
     gene            14738..>16089
                     /locus_tag="VF222_08830"
     CDS             14738..>16089
                     /locus_tag="VF222_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7142569.1"
                     /translation="MISDLKTLINIPSVSAKDQNLVECAEAVFSLMNRVGIKSEIIYL
                     DPKEKNIATSSSSLTPPVVYGEIKSQKNPNGKTILFYNHYDVQPEDPIELWENNEAFS
                     GNVIGNYIYGRGSSDDKGELITRIKAVEYLLKSSGDVPCNIKFIVEGEEEIGSPHLER
                     FIIQNKEKLKCDGVIWEFGYIDEKERPIIMLGLKGILYVELIAKGGPSRDVHSSLATL
                     IENPSWILINALKILRDIDGKILIKNWYKEVREFSQEELRILDKEPFDEEIFKREYGI
                     SKFLNNVNGIEAKKFYEGMPTCNISSLISGYIGKGSKTIIPNKAIAKLDFRLVPNMDP
                     VIQFKRLCDHMIDHGFNKNLLEIKLLQKEWPARTTPVDHPFIKVVEKSANTIFGSTIK
                     SISSAATGPMYYFTNILDIPCVCIGSTYKYGKSHSPNEFARLDLLNKTTKWISRIIEN
                     "
BASE COUNT         5531 a         2453 c         2308 g         5797 t
ORIGIN      
        1 aaaaatatta aaagaatgtt ttgaaatttc cgataattat tctcaaatta gaactaaaat
       61 tcaaattgat tctaaattac aaaaaactat tttggaaaag ttatatgacg aatttaaaaa
      121 agattcaaca gaaattaata cactagatgg aattaaaata attatagatg agacttcttg
      181 gattttattt aggatatcta acactgaaca tgttttaaga atatcaatag aatctactac
      241 taataaaatt aatactcttt atagaaatgt aaatgaaaga ataattaaaa tacatgacca
      301 aattaaatga gaaagaaatt atagatctat tttattctaa tcttttagaa aaaaataaaa
      361 taaaattcca gataagagat gatgtatcag tattatcttt taaagaccta aaaaaaaata
      421 aaataagatc tttcaattcc tctaatataa ttataaagtg tgatatgtta gtagaaagta
      481 ctgatgtagt taaacaaatg aaaatatggc aaatagcaag aaaaagtatt gtttctgctg
      541 ttagtgatat gtctgcaaaa ggaataaaac caccatattt ttcgttacta gcattaggga
      601 ttcctagctc ttggacaaaa ataaaaataa aaaacctaat tttgggattt aaaaaagcct
      661 ctaaagaatt tggtatagtt tttcttggag gtgataccaa tgaatctaag gaactagtca
      721 ttgattgtac tttagttggt tttttggatt ctacatctaa cactattcct ttgagaaata
      781 atgctaaagc tggagatctt gtaataacat caggagaatt tggttatacc tcagctggat
      841 taaaaatctt gcttttcaag tcaaaagcaa taccttcttt taaaaaacaa gctatatcaa
      901 gtattatact tccaaaacct aatcaaaaat ttggaacatt attgggaaag tatttttcat
      961 catcaattga ttctagtgat ggattaggga tctctcttta tgaattggct aagcaaagca
     1021 gggttaattt ttatgttgat gatatcccaa ttcctagagg tttgattgaa tttgcaaaaa
     1081 gtaattctct cgatatttat gatctaatat ttaatggtgg tgaagaatat gaaataatag
     1141 ctacgatcaa tccctcgaat ttgcaaaaag taagtcttct tgcaaagaaa tttcatttac
     1201 caatgtttgt aataggaaaa gcaattactg gaagtggtaa tgtttttgtt aaatcggata
     1261 ataaacttga attgtttcat ggaagaaaaa ttgaaaaaag taaagattat tttctattag
     1321 aaaataaggg atttttacat ttttcataat atattaacaa agttttaata tccaaattaa
     1381 gtgaattata ggattatgtc tgaaactgaa gatattactc agtatagatg gggagtagct
     1441 catattttta gcagttataa taatacttta gttcatatta ccgatattag tggtgctgaa
     1501 acaatatcgt ttagttctgg aggtcgacat gtaactgctg atagatacga atcatcacct
     1561 tatgcagcaa tgaaatctgc cgtatctgca gcagatgcag ctaaatccaa aggaataaat
     1621 gcacttcata tcagagtacg cgcagttgga ggagtaggac ctagaatacc cggtccagga
     1681 gctcaagctg caataagagc attagctaga gcaggtttta ggatcggaag aatagatgat
     1741 gttactccag ttcctcacga tacaaccagg aaacctggtg gtagaagagg aagaagagtg
     1801 taacattaca atacttttta atttttctta agtagaatat aataaagctt aaattataaa
     1861 aattaatatt tatagagata tagttataat aataagatta actaaattac tattatcatt
     1921 ttttaatgat atacaaaata aattaaaaat aattatttga tgaaatacct ttatttattg
     1981 acattatctt ttattttctt catcatcaat ttctttattc tgcttaagtt tctctgcaaa
     2041 tgacatatat tttccatgtt cttcaatttt gatagtagta tttattttaa cattcatccc
     2101 tattgtgcga aataacgata gtaattctcc tcccgaaatt tttcggttta tttctccact
     2161 attaataagt tctactattc tatttattat tgctctagtt tctgagggaa attgagattc
     2221 tgctaaattt aatacttcat ctcctcggtc atacaaatat gatagtaaga tctctctttc
     2281 agaaggctcc cgttttttaa tatctttttg tatgttttct tttttctgtt tcatcatacg
     2341 ttgcattatt tcttgcattt tcttagcttt aagaatttca atatctggat cttctcccat
     2401 ttaaccttta atcactccta tcggaattaa ctttgctact ttagttgcaa tccccaattt
     2461 atctgataca ttcactacgg cgtctacatc tttataagct aaaggtgttt cctctactat
     2521 tccatttttt gttaatgctt ttatatagat cccttttgca tcaagttcag cttttacttt
     2581 ttcaacagta taactttttt ttgctgcacc tcttgacata gttcttccag ctccgtgtgc
     2641 agttgatcca aaacttaaat caagagattt tttatttccc actaatatcc aacttgctgt
     2701 tcccatcgat ccgggtataa ttacaggttg acctatttgt cgatatttct ctggaacctg
     2761 ttccatacct gctggaaaag ctctggtggc accttttcta tgaattacta aatctctcat
     2821 tccttctcca tcaaccttat gacgttctac tttggctata ttatgtgcta catcataaac
     2881 caatttcata tctaaccctg tttctgaact cgaaaatatt ttctcgaatg tttttctagt
     2941 aaagtgggta attatctgcc tatttgacca tgcaaaattc aaagccgaat acatcgcttt
     3001 tctataattt tctccttcaa ttgaattatt tggaacacat gctagttctc tatctattaa
     3061 ttcaatattg tattttttca aagatttttc agaataacgt aaataatcac tgcaaacttg
     3121 atgaccaaat cctcgtgatc cacaatgtat tagtactgtt atttgacctt tttctaaccc
     3181 catcaccttt gctgcatttt catcaaaaat ttcttggatt tgttgaattt ccagaaaatg
     3241 attccctgag cctaagctcc ctaactgagg agctcctctt tttctggcag tatcagacac
     3301 actttctgga ttagctccta tcattctacc tttctcttcg catacttcag aatcttgttt
     3361 atatccataa ccatgttcaa ttgcccagtc aactccttct atcaacaatt catctaattc
     3421 tgatctagtg agtttaatag ctccatcgct acctactccc aatggtattg aattaaataa
     3481 ttcatttact aactctttta aatgaggttt aacttgattt acgttaagat ttgatctaag
     3541 tagtcttact ccacagttaa tatcatatcc tacacctccc ggactaataa ctccctcttc
     3601 catgtcagta gctgctactc ctccaaccgg aaaaccatat ccttcatgac catctggtaa
     3661 tacaactaca tgttttctca caccagggag tgttgaaaca ttgactgctt gatctaatgt
     3721 cctatcctca ctcattttta aaatcaagct atcatctgca taaatcgtaa ccggtacatt
     3781 cattttttta gaatcatcaa cattaatttt aaactcaaat tcgtttaatt tttcaataac
     3841 atgatttcca cgcttggtta ttattttatt cattgtattt ggttattttt attatataat
     3901 attattataa gccttgcttg actattaaat aaattttatt aaagcttaaa tatatgatcc
     3961 aaatctttta atctacaatt gagaataatt aaagattgtg atactgtaga atatcctttt
     4021 ccttcaacaa gagtaatagc atttcttcca ccttgataat cgacttttta tgccctaata
     4081 ctattgtgct ccagcttttt ctatttcttc atcgatttct atatattaat taattgtaat
     4141 aactacaaac atacacaaac cgtttattat ttctagtaat ctaggcaaat tgaaacattt
     4201 tcttgtgtta atatttgtaa aattagattt ttttcttcta atcttttcat ctttaaatat
     4261 tctaaataaa taaatcattt tggatagaaa ttatcaactt ttgatctcta gtttattact
     4321 attatacgga caaaaaaata atcactatta taatctttat ttttttatta gaggttcctt
     4381 atcattgtat cttattaaaa taatcctctc tcctctaagc tggaaattgt caaataatat
     4441 aataattgtc ttatgcataa tttaatttta ttatattatg tcttctagct tttgactttt
     4501 gacatatatt gatcagtcaa tacttcattt attatacatg atgtatgtaa ctagccttat
     4561 ctgatattga ttactctggg catttcaata ctcacagtta ttttaatgat aaattttcat
     4621 taagtctgct cttataaaag aggactgatt catcctctct aaagagacaa tatttatatt
     4681 aaaatatctt tctaaatttt tacttttatt aataaaaatg aaattaatac ttgtgtattt
     4741 atattatctt cattaacaat aaataaatga aattctagaa tgtttactgg tataatagaa
     4801 tctctgtgtg aaataaaatc tataaacgaa atgaaaaaag gtaattcgaa gtatcctgct
     4861 gaaattaaat tttctatcga tattgggaat ttaagtaagg gtttaaagat tggtcaaagt
     4921 atagcagtaa atggagtttg tttaacaata accaaattaa ataataatat tgccgaattt
     4981 tcagtaattg gagaaacagt aagaaaaaca tgtctagatt caataaagaa aggcgataga
     5041 gttaatattg aaagaagtat gagagcttct gatagatttg atggtcattt tgttcaaggc
     5101 catgtagatt gtacaggaac tatccacgac aaaatcgttt ttcccacaga aacaaaactt
     5161 attatcaaaa tctctgaaga actaaaagaa tctttccaat atatagtacg aaagggttca
     5221 attacaatcg atgggatcag cttaaccgtt gttgatattg ataataaaaa cttattatcc
     5281 atatctttga ttcctcatac attaaaaaaa actagtttag gaataaaatc aatcggagat
     5341 aaagttaata tcgaatttga tattattggt aaatatattt caaagctttt accaaaaata
     5401 tagtagtaac aaatttcgta gtaaaatata taaatactgc tgttttataa attgtgtttg
     5461 tcaactgtaa aagacgccat agctgcactc agtcaaggaa aatttgttct tattcatgat
     5521 gatgacagta gagaaaatga aactgatatg ataatagctg cagaaaatat aaagccatac
     5581 catatagcaa ctataagaaa tgacgcaggg ggcttggtat gtctagctat agacggttat
     5641 atttctacaa agttgggatt atcatacata catgatattt taaactgtgt ttctaatgat
     5701 catcctttat ttaccactat gacaaatggt cgttctccat atggagacaa accatcattc
     5761 tcaatttctg ttaatcatag ggatacttac accggaatta ctgattatga tcgtgcatta
     5821 acaatttcca aacttgcatc agtttgcaaa aatattgata atggtggagt atcagaattt
     5881 attaagtgtt ttagaactcc aggtcatgtt tctcttttga tcgctgcaaa aggtctatta
     5941 aaagaacgat ttggtcatac agaacttgct gtttatttgg caaagattgc aggtctaacc
     6001 ccagctgttg caatatgtga aatgcttgat tctcacactt ttagatctct ttcgatagat
     6061 gaagcaaata aatatgcaaa attaaaagat attcctttat tagaagctga tcaattaaag
     6121 acatattcgg tgatttaaat ttcaattggt caaagttcac ttagcaatag ttgtttctga
     6181 atttaatcat gaaattactg atctaatgtt agaagaagca aaagttcatg catcaaagtt
     6241 aaactcgaat gttagtcata tttgttacgt tcctggaacg tttgatatgc ctctaataat
     6301 aagtcattta ttacaaaata atactattga tgctgtaatt actttaggag cagttataaa
     6361 aggtgataca aatcatgata taataattgc tgaaaataca gcgagattaa tatcagattt
     6421 atcattacaa tataaaaaac caattgcact aggtatatca ggacccaaca tgacgattga
     6481 acaagcatgg gaaagagcaa aaatagttcc catacgtgca gtaaatgcag ctgtaaatat
     6541 ggtaaccaga ataaaaaaat taaatgaaag tcaatatagt caaaataaaa ctactattat
     6601 tataaaatga atatacagat aacaataatc aaaatagaag gttatgggcc atggacattg
     6661 gtcttgggta gtgacagaga agcaaaatta caaatgctcc aagctcagat ttaccatgac
     6721 ttgcaatatc tattctccaa atataattgt ctggtattct tcaatagatt tgatgaatat
     6781 tttgtaatta caaatacgtt atcattagaa gatcatgtct cagttataaa agagatttct
     6841 attaaacatc caaacttgtg tatatctttt acaataggaa atggaaagac tccattagat
     6901 gcaaatatcc aagcgtacca gtcaagaaaa aatgaaaaat ttcttgttaa aaaatttcag
     6961 gtttatggtg aaataaataa taatctttca gtaaattcct ccttaaatga caaaaaagta
     7021 aaaatacttc atattgatat aaatgattca tcgggttttg gaaaccattt atcgccttat
     7081 gaaactacta ttaaggtaat gaaatcttat gttcttctta tggataaatt tctcgagaaa
     7141 aattcattaa ctttttttct aggaggagat aactttatga taattgctgc tgatctaata
     7201 gaaaaagatg aaatttctaa tttattaaat agtatttctg atgaattgga cattaaattt
     7261 aattgtggca taggatttgg aagaacatct agagaatcag ccaagaatgc cacagaggct
     7321 ctcgattcca ttagaaaatt gcgtaagaaa ggagaaatta ttcctatcta tgagatcaaa
     7381 tgactctttt tattagaaat atcagtctat tatatggaaa agacttagtt tacataaata
     7441 aaggatttat atgcattaat aaaaatggaa taataacaga agctggaagt gaagacacat
     7501 attttgataa gtataaattt acagatacaa aagtttatga tggaacagga tttctggtca
     7561 tacctggttt tataaattct catactcaca tagcagattc aataggaaag gatatatcat
     7621 ttagttctac ttttaatgaa atgattcatc ctgtttatgg aattaaaaag aaaattttga
     7681 ataaaagtac caaagagcat ctaaaatact ttatacgaac ttctgccatt tccatgatga
     7741 taaaaggtac aatatctttt gtagattttc gtgaaggagg tccagaagga attgagttat
     7801 taaaaggctc tataggtgat ctacctataa aatcagtaat tttagggaga gtagaacaat
     7861 attttgattt aaaaaaaata ttggaaatta aaaaacaaac taaagaaaat agtgatgaga
     7921 aattagtaga taaattacaa aaactgtctt tggagtctct ttcaagagta ttagaattaa
     7981 gtgatggatt aggactaagc ggggcaaatg aaaatacgga tgatgctctt aaacaatata
     8041 tacataaagt taaagaattc aataaaaaaa ataattatgt atatcataga aaaaaaataa
     8101 ttgctattca tgcagcagaa tccaaaagta ctatcaaatt ctcaaaatta cttacaggaa
     8161 aaacagaagt tgagaggata attaattttc ttaaaccaga tatcctgatc catatgacac
     8221 atgcgacttt tgaagatcta actcttgctc tacataataa cgtaggcata gtattatgtc
     8281 caagatctaa tggaattctt ggaaatggaa ttcctgatat tgtgaccatg gtaaaattag
     8341 gatgtaagat atcgattggt actgataata taatgcttaa ttctccggat cttttacagg
     8401 aactagatta tttatggaaa gtaattaaat cacattcatc taaatattct aattccattg
     8461 acccaaaaga attattaaaa atgattactg ttaacccggg agaaatattt gatttgaatt
     8521 ctggatcaat cgacagtgga aaatatgcgg atcttttatt tatcgataag tatcacatag
     8581 atttacaacc aattcataat atctattctt ctattattca tagactttct tatggggcaa
     8641 taaaagctat tatgatcaat ggcaggttta taaatgaaac tccctactga tttaaaagta
     8701 attattaatt ctgctatgac tttagatggt aaaatctctt cttatactgg caattcacgt
     8761 atctcttcga agaaagattt agtacgcgtt cataaattac gctctaaagt tgatgcaatt
     8821 gtagtgggta taaatacagt tcttatagac aatcctatgt taactgttag atttataaga
     8881 aataatagaa tttatccggc tcgtattatt atagacagtc atggtagaat accattagat
     8941 tccaaaattc taaaaactgc tgctacaatt aaaactataa ttgttgttac aaaacaggca
     9001 agtaaagata ctatagaaga aataaagaaa ttaggagccc atgtcataat aattggatca
     9061 aagttaataa atttaaaaaa acttttcaat atgctataca agatgggtta tagaaaaatc
     9121 cttatcgaag gtggaggaga attaaattgg tcttgtttac atgatggaat tgtaaatgaa
     9181 ttaattataa ctattgcccc aatagttata ggtggaagaa atgctattac tcttgttgaa
     9241 ggaaaaggat attctacaat atcacaaggg ataaaattaa agctgactaa aattattcaa
     9301 aataaaaatg ataatgaaat aacgctttat tataatagct aaatactaat tatttttctg
     9361 tagatatttc agatagtatt tgatcaattt tatatctcaa tgcttctttt gtatagttat
     9421 tgtctaccat agcaaggttc tccttacaag tagggcattt aaaaaaagac tctatagcta
     9481 tatcaaattt tattctttga cattcattat ttccacaatg ataaaattct gatgattctt
     9541 catattctaa tcttttttgc aatctatcca gaattttttt tttctgatta tctataaaat
     9601 tgtcaatttg atcacttttt gcacgccaac gataaacaaa ccagcctttt ttctcatctt
     9661 taactctaat tccagtaatt aatgcttttc caaacatatc atataatact tttctaataa
     9721 cattgattct cattcctgtt gcaccagcaa tttcttcatc agttgcatct tctgtattta
     9781 aaagtgcttt agcaaccttc tgatattctt ctccacctat taaattggat attttggcaa
     9841 aagattcttc attatcaata gatggcataa caatagtata tgttgtgatt caggtattat
     9901 atatttaccg aagttctcca ctttatttta aactaattat aatttattaa acaaatatta
     9961 caaaagaaaa tcgggaagtt ttatttagca accttaatta tgtgtcaaaa attggaaatt
    10021 aatgcttcct aaaatttcgg tttgaagatt ttttatagtt gtagtttttt ttcttactgt
    10081 ttttataatt atgttttgac catttgttaa agggcttatt tttatttaaa taattgtgtt
    10141 ttttaaactc tttttgaatt ttaggtattg gaatgcctat ttcctcattt aattgacgaa
    10201 ctttatattt agtaaatctt aaaatttttt caaaatctga aattttttca atcgcaacta
    10261 gagatatagc tttgccatca gaatttgctc ttgcagttct accgattcta tgaaaatagg
    10321 ttataggatc cctaggaata tcataattaa tgacatattt tatggaagga atatctattc
    10381 ctcttgctgc aatatctgta gccactagaa tttgctcttg ctcatctcta aatttatgca
    10441 ttgcataatt tctttctctc tgagacagat ctccgtgtaa tgaagttact tttaatagtt
    10501 ctttttttct taaattcata gctagttctt cagccttctg ttttgtagaa gtaaaaatta
    10561 acatctgatt attattgctc tgtttaatta aatcacacaa gtgattatat ttttcttttt
    10621 catttgtaat taagtacatt tgttctatag tttttaatgt aatatcatct tgatttactg
    10681 taatgtgtat tgggtttttc atataccttt tactaatttc tattatctct ggaagtattg
    10741 ttgctgaaaa aagacataat tgttttatat gttttacctt tgataatata taattaatat
    10801 cttcgataaa tcccatatct aacattcgat cagcctcatc taatactaca aaatctattg
    10861 tatccaagta tattgttttt tgtcctatat ggtcaattaa ccttccagga gtagcaacaa
    10921 taatgtgaat tcctctttcg agtctaataa tttgatttct catattttgt cctccataaa
    10981 tagcagccac ttttgtttct gtgtattttg caagtttatt catttccttc gctacttgaa
    11041 ttgctaattc ccttgtagga actaatacta aagcttgtgc atttcttgca tttttcattt
    11101 tcattaaaat aggtaatgaa tatgcggcag tctttccagt tccagtatgt gcttgtccaa
    11161 ttacatcgtt tcccttcaag ataataggaa ttgctttttc ttgaatagga aagggtgtgg
    11221 tatatccact ttcttttaga gctagtagca gttgtttatt tagtcccagt tcatcgaata
    11281 gtcctgtcat taaatttcac ttagtttatt aaacaatctt tcatcttatc taatctaaac
    11341 cttttgtgaa tttgtaaaaa atatatgatg atattattaa gtaattaatc ttttttaaaa
    11401 ataataataa taaaataaga agataataca aacactaata caactgtttt tgcgttatta
    11461 tgatgatata tttaatgatg gcggaagacg aagaactaag agagaaaaag ctacggggct
    11521 ctggaggcta tgttattgcc agaatttcag attatgagca aaaaaaagga aatctgggtg
    11581 gacccgagtt atttatagca ggaattggac gattaccaaa agacagaatt ttaaaatatt
    11641 attgcaataa atgtgagaaa gactatccaa catccccaga tctaaattat gaaaatccaa
    11701 atgaagatgt cggtgaaggg gttgttttaa ttgaaaaagg tgaatacaaa tgctcagttt
    11761 gtaatgcagt tctttctcaa tatcgtaaat ttaataatga aacccctcct cctcctccta
    11821 gtaaaactaa gtctttctct caacaatcaa actcatcgga tgtcactaaa tctgtaaatg
    11881 atatttccga ttcgtcacct aaaaataaaa gcgaatctga aaattctata ctaacctcta
    11941 ataatactca tgaagaaaat atttcagatt caatacaatc tgagagtaca gatactaaat
    12001 ccagtaatgt aaccatcgct aaaggaaaat attttcctat tcagtcaatt attggaatgc
    12061 cagtttatga tcatgaagcc atgctagtag gaaatgttca agaaatagga cttagaaaaa
    12121 cgcttaatgg tacaatacaa attacattaa aaattgataa tcgcgataaa atatctgaaa
    12181 ctaatggtga tgaattatat aatgaaatta cttggtctga tgtttctaaa attggtgata
    12241 ttgttctaat aaattgggaa caaaagaaaa tatcatcatc taacacatct cctagagata
    12301 aaaaaatatg tgttgcatgc aaataccata atgaatcaga tgctatgtat tgcgaacaat
    12361 gtggtaaaaa actagaataa tatttttctt tcaacttttt aatattatat cgttttatct
    12421 ttctgatttt ttaatcctat tcgaacatct actgctctta caccgttacc tttttctaat
    12481 acaagtaatg gattcatatc aaattcttca atttctggaa aatcagtaac taattgtgat
    12541 agtcgttgaa tactttctac tattccttct atatctgatt ccttttctcc tcttgctcct
    12601 tttaatagct taatagtttt tatagattct accatttctt ttgcttgttg ttcatcgatt
    12661 ggagctattc taaatatgac atcttttaga acttctacat atatcccacc caaaccaaac
    12721 attattaatg gcccaaataa tggatctctt tttgcaccta aaattgtctc ttttgcagaa
    12781 cctatcattt cttgaactaa aatccctttg atctctgcgt ctgatttgta tttttttaca
    12841 tttaataata tttcattaaa tgcttttata gcttcttgct gatttttaat tccaatctta
    12901 acacctccag catcggattt atgaatgatc tgtggagaaa caattttcat aactactgga
    12961 taacctattt cttcggctgc tcttgccgac tcttcttcat ttgtagccag aaattttttt
    13021 ggagttgaaa atccatatgc ttttaatatt tcataacctt cttcttcaag caaattagtt
    13081 ctattagatt ttatcacatt ctctaaaaca tctcttgctt ttgtcttatc tgcctcaaat
    13141 ttttttggag gaatatcttc tttttcaatc catctacggt gtctaaacat cgcttctaat
    13201 gttcttattg ctggttctgc atacataaaa tgcggaattc ctccctctga aagaattttt
    13261 ttattttcaa ttccttcagc taatcccatt aaggccgcca acattgtttt tccagaatct
    13321 ttagatgttt ttattattgt ttttgctaat tcattataat ctaatgttgc agaaggtgta
    13381 cacatagtaa cgactgcact aacattatta ttagataaga cttcatctag cactttttca
    13441 aatcgattaa aatctgcatc tcctactatg tcaacagggt ttctagctga accatgaggc
    13501 ggtatgactt tggctatttc cgattctgat tgagaaatat ctgccatttt taatccatat
    13561 tttgaacaag catctgtgga aattatggct ggtccacctg catttgatac tatgactact
    13621 ccaccatttg aaggcggtat tggttgtttg gcaaaagctg tagatgtttc aaataattct
    13681 tgcatggtgt ctactcttat aactcctgat tgttcaaata aagcatcgta cgtttcatca
    13741 gaccccatca aagcgccggt atgtgacata gcagcttttg ctccttcagg tgttcttcct
    13801 gatttcaata caagaatagg tttctttttt tcttttgtta tttttttagc tatttgcata
    13861 aatctttttc catcatgaat atcctctaga tacatgacaa ttactcgtgt atcatcatct
    13921 tcggaaatta gttctaatat atcattttca tccatatcta ctttatttcc catactaatt
    13981 acttttgaaa agcctattcc ttgatctata gcatcttcaa cggtagcagc gcatatagca
    14041 ccgctttgtg atactagagc tattcctcca acatctggcg taatttttaa aaatgtagca
    14101 ttcatcatac tgctttgaga taagctcatt attcctaaac aatttggacc gattattctt
    14161 attccatatt gtttaccaat atctccaatt tgcttctcaa gttgagatcc agattcatca
    14221 acctctttga atcctgcgga aactattatt gcccctttga tattcttttt tcctatttct
    14281 ttcattactt gaggaacagt cttgtttgga gtggctatta ctgccaagtc aatatcttct
    14341 gatatatcta gaacgctttt aaaagcattg attccaaata cagtaggatt agatggagta
    14401 ataggatata tttttccttt ataaccattg atgatatttg aaaagatagc ctttcccaca
    14461 cctggttttt cagaggcccc aattacagca attgactgtg gattaaagaa aatattcttt
    14521 gagtcaaatg aggatgatga agacgtcata attatcgttt cacaaatgca tggttatatg
    14581 tattcctgtt aagattatat taaaatataa taattccata attcaaacca tatcattagc
    14641 aatatgtact tttttatgaa aagttaaatt attcttttac aaattttaaa tattggtaaa
    14701 ctcgatagaa aagattgttg attcagagat tagtacaatg atctctgatc ttaagacatt
    14761 gattaacatt cctagtgttt ctgcaaaaga tcagaatctt gttgaatgtg cagaagcagt
    14821 tttttctctt atgaatagag taggaattaa atcagaaatt atttatcttg atcctaaaga
    14881 aaaaaatatt gcaacttctt cttcttctct tactcctcct gtagtatatg gagaaataaa
    14941 atcacaaaaa aatccaaacg gcaaaactat cttattttat aatcactatg atgttcaacc
    15001 agaagatcct attgaattat gggagaataa cgaggctttc agtggtaatg ttattggaaa
    15061 ttatatttat ggaagaggtt catcagatga taaaggagaa ttaattacaa gaataaaagc
    15121 agtagaatat cttttgaaaa gttctggtga tgttccatgt aatataaaat ttatagttga
    15181 aggtgaagag gaaattggga gccctcacct ggaaagattt attatacaaa ataaagaaaa
    15241 actaaagtgc gatggagtaa tatgggaatt tgggtacata gatgaaaaag agagaccaat
    15301 tattatgttg ggccttaaag gtatcttata tgtagagctt atagctaagg gaggaccatc
    15361 acgagatgtt cactctagtc ttgcaacact aatagaaaat ccttcttgga tccttataaa
    15421 tgccctaaag attcttcgtg atatcgatgg aaaaatttta ataaaaaatt ggtataaaga
    15481 agtaagggaa ttttcccagg aagaactcag aatattagat aaggaacctt ttgatgagga
    15541 aatattcaag agagaatacg gtattagtaa atttttaaat aatgtaaatg gtattgaggc
    15601 caaaaagttt tatgaaggaa tgccaacgtg taatattagc agtctaatat ctggatatat
    15661 tggaaaaggt tctaaaacta ttattccaaa taaggctata gcaaagttag attttcgttt
    15721 agtacctaat atggacccag tcattcagtt taaaagactt tgtgatcata tgatagatca
    15781 cggctttaac aaaaatttgc ttgaaataaa attactacag aaagaatggc cagcaagaac
    15841 aactccagtt gatcatcctt tcataaaagt tgttgaaaaa tctgctaata ctatatttgg
    15901 ttctacaata aagagtatct catccgccgc aactgggcca atgtattact ttactaatat
    15961 tttagatata ccatgtgtct gtatagggtc aacatataaa tatggtaagt ctcattcacc
    16021 aaatgaattt gctcgattag atttattaaa taaaacaacc aagtggatta gtagaataat
    16081 tgaaaattt
//
LOCUS       DASVEV010000196        10925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1055561, whole genome shotgun sequence.
ACCESSION   DASVEV010000196 DASVEV010000000
VERSION     DASVEV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055561"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF222_08835"
     CDS             complement(<1..236)
                     /locus_tag="VF222_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142570.1"
                     /translation="MNNKKDNKEENDLPDASNFFHISYNDGKDNRNDDSRFLAFSSTG
                     SGPPDDKPVGPPDDKPVGPPDDKPVGPPDDKPVGP"
     gene            complement(305..544)
                     /locus_tag="VF222_08840"
     CDS             complement(305..544)
                     /locus_tag="VF222_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HEX7142571.1"
                     /translation="MRKKKGKLQEILSKAIYADNPHIYIISYRDFDTILSVPLLKFME
                     VSQNFEIIPATRIIQVRKGERIMYEKRIAGKKKEI"
     gene            641..1066
                     /locus_tag="VF222_08845"
     CDS             641..1066
                     /locus_tag="VF222_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142572.1"
                     /translation="MELSKIFEHPILIYDDKCSSCYKFALLIHSLSNGWIRIEGHYYS
                     SNIHLINKILFPANYDPKKMFWLVNKKGAWGARSALIPLIVEVIRGRLNSHFVIKRNS
                     TMDNTYSCRLDPNSVCSDYLNIFKRILGVLRNTGKFPFN"
     gene            complement(1085..1438)
                     /locus_tag="VF222_08850"
     CDS             complement(1085..1438)
                     /locus_tag="VF222_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX7142573.1"
                     /translation="MGIYTESRPWGKFEKFHENENCTVKLIYVNANSRLSLQYHQHRS
                     EFWKIVKGKAKIDIDKKELVVKENENITIPCGAKHRITALEEDCIILEISYGKFDEND
                     IIRIEDDYNRQTSAG"
     gene            1564..2127
                     /locus_tag="VF222_08855"
     CDS             1564..2127
                     /locus_tag="VF222_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142574.1"
                     /translation="MSEIHFTEGNLIILYSIHKNSSLQIPNHILERIEHCNRLYQIIL
                     KSKPDSNKTIILVVAEPHYSELIKKELINRQIKEEIIIIDSDSNKVSHTFDNLKELLK
                     HRINPPVVYFVGSVWLKDVFDSIVKSQFKEYKIQFEGALDHRPVMEVEHDKSLETPKK
                     GKEHFKQVIKNKAFDMLLNYIFPEDHK"
     gene            complement(2204..2911)
                     /locus_tag="VF222_08860"
     CDS             complement(2204..2911)
                     /locus_tag="VF222_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX7142575.1"
                     /translation="MNRRISCLIGSSFVILLLYFGLTENYKNSILNNIEIFAQENKSP
                     VVKNSKIIDFSGVNYADVVNSRDINLNGFTVSVWFNTVMNVTSGNNAFLLNKGGFGSD
                     RAGFNLNYGIWLNNREQVGGGFETSSGEDNYITSQGSYADGVWHNAILTFDEKLHLLK
                     LYIDGLEIATNSTKVGITPDNTGKQTIRLGANSLAEKGKINGNYTGQLDDIQVWNFAF
                     TKEQVADLFTKESKLAR"
     gene            complement(3012..3332)
                     /locus_tag="VF222_08865"
     CDS             complement(3012..3332)
                     /locus_tag="VF222_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142576.1"
                     /translation="MKPNSFAMREWHLEHVEKVIVRYVKGLSPTATSFEKRNFKKYST
                     MSNCAKQIEYDKKHGVTEEEVIAVMQRIRNDDSFSELRKNPGSVQRLDELEKQLTSPK
                     KISW"
     gene            complement(3467..4840)
                     /locus_tag="VF222_08870"
     CDS             complement(3467..4840)
                     /locus_tag="VF222_08870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX7142577.1"
                     /translation="MQKDNSNTTNIHNDLRKFLELLENEDEIIRIKKNVNTTFELPAI
                     VSKLDGNKAVIFENVEDKTIPVISNLIGTRKRFALAIGSKEKNDIHTIINKSINNMNI
                     KVKTVNDIPYYQNSSNKLSDLPIVTHFENDAGPYITSSIVYAKDQEKGNQNSSTHRLL
                     KLDDKNMAIRMVEGRHLHKCFMYAKDHGEDLKVAIVIGVHPAINIAAAYQASYGIDEM
                     HIANTLLNGNLQLGINKFSQLLIPSLSEIVLEGKILCDKTHEEWMVEMLKTYDFRRQQ
                     PIFELEKIRFRNNTIFHDILPGYSEHRLLMGLPVESKMLDQVKNVVPSTSQVLLTDGG
                     SNWLTAVIQIKKRVEGESKNALLAAFAAHPSLKIAIVVDEDINPANPVEVEYAVSTRC
                     QADKDLIIIQNAKGSSLDPSSDQVNLLTTKLGIDATATLLKVKERFEIAKIPGEENLK
                     LSEYLSE"
     gene            complement(4827..5192)
                     /locus_tag="VF222_08875"
     CDS             complement(4827..5192)
                     /locus_tag="VF222_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142578.1"
                     /translation="MEDKGDMLVKDIQIDDKRIVHIKSIFFENGCFIIISENSNRIGS
                     VTMASSLSNKVTSAIIIPSKFDSVFINSIAQRISLMINGICLVSLHIKTPLDVKSMKT
                     IMGEILNMIGTQAEKDAKR"
     gene            complement(5496..6146)
                     /locus_tag="VF222_08880"
     CDS             complement(5496..6146)
                     /locus_tag="VF222_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142579.1"
                     /translation="MREAINHKITVATLNGKSYYKIINTLKLLQLNYEELSPSEALNS
                     YSTIIITSKEESPIFKTKNIIVDSELNENPVIIKAKILRKLIKSHTYDQLTIGIDPGN
                     RIGISIFYLYDEIESVVLSTIESTLNFIFKILAEINAKKKIVRIGDGNINMARSIAFL
                     IKSNFKDLVHVEIVNEHGTSSIQNNESNRRILKDKSSARLIAFRNGKIFDPKLDHN"
     gene            7500..8489
                     /locus_tag="VF222_08885"
     CDS             7500..8489
                     /locus_tag="VF222_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7142580.1"
                     /translation="MESDIKSQKSQVYQYESIVNDSLLWIDIRNPTREKMNNLGRRYS
                     FHELNIEDCLSKTQIPKIDKYDNHVFVILHFPTGSTDDKIPRVTQLSIFIGTNYLITV
                     HQDDLKPLVEMFDLCKKEKNQEYRKVMMGKSSGYLLHTIIDALVDDLFHILMKVIGNL
                     DDIEDAVYDSKIGIAREISHIRREITTIRRVSIPLKRIIVSLTRDIQKISEEDLTPYF
                     DDIKDHIDKVLDTLEEAKETIEIYKDTDFMLSTEKTNKIIAMLTVVFTLSIPITVIST
                     IYGMNISLPGSDLNRFQFLGEYTTLIIVMLISIIITGIMFISFRRLGWLARRI"
     gene            8518..10335
                     /locus_tag="VF222_08890"
     CDS             8518..10335
                     /locus_tag="VF222_08890"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX7142581.1"
                     /translation="MSLELLRTKITNKGQRITPLFCLVSADNLSIAQKLITEFESSYN
                     KKETKGDLQKRLFLYENSYSDFKLIRGLITLLERRCVFKINQFIPTGYKNHFFSTQLP
                     SSFSLRKVLFEESSRGGLSLDNTKRDKIFQHVASKLGIDTNYLEKLMWLDQEDYLILE
                     SFSVIKPIHLLGIYNLSILQTLLFNSVTFEFTIKGGTNWKQVLRTIKKFGLMYNLRKI
                     QKNSDNIFPKEIQYNQIEQNIVDGDDFKSYFNESIVCSIDGPLSIFKLTNKYGILIAK
                     VIPKIISASKWGIKASIIKNSLAGRKLYDFDLSSDSEVYFFNPINDRFYNNYPFEDSN
                     SINLDFDSSVEAKFAIQFEKFRTGWKLVREPDPLILPGGRAFIADFLFERYGKKIYFE
                     IIGFWTPQYLERKFKKIYEIIKLSNNKYDLLIAINENNLVSESGEMKKLLSGSILDYD
                     KIIIYKKDSIPIKKILFYLKSIDSKIMNQNLETYRSAMTEYIVDLLNKNQEIIDLEEI
                     SKTYGVSINSISNIISNLDTDNHIKKYIIQNSLLISERKLNKIKDRIGDIDNLIKIQK
                     IFENNNIPIQYTIDILKYLGYEIVWKGMDSSNIIIKRKT"
     gene            10437..10766
                     /locus_tag="VF222_08895"
     CDS             10437..10766
                     /locus_tag="VF222_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142582.1"
                     /translation="MNNIKNIKINNNTWLQNISGNWRDIECPCGSCYHFNGSLEELFN
                     WENIHLRHMASDYDNHAMISFYPDDMVIFYHSKTGYVIERRKHKTYELNEIKNAWIHG
                     KITITGA"
BASE COUNT         3888 a         1528 c         1394 g         4115 t
ORIGIN      
        1 ggaccaactg gcttgtcatc tggtggacca actggcttgt catctggtgg accaactggc
       61 ttgtcatctg gtggaccaac tggcttgtca tctggtggac cacttcctgt tgaactaaaa
      121 gcgagaaatc tagaatcgtc atttctattg tctttaccat cattataaga gatatgaaag
      181 aaattcgaag cgtcaggaag atcattctct tctttgttat cctttttatt attcaatata
      241 tgaattacaa agtaacataa tttaataatt ttcattaaaa ttcataataa ttcttatttt
      301 tatgttaaat ttcctttttt tttcctgcaa ttcttttttc atacattatt ctttctccct
      361 ttctgacttg tattattcgg gttgctggaa taatctcaaa attttgtgaa acctccataa
      421 attttaaaag aggaactgag agaattgtat caaaatctcg atatgaaata atgtatatat
      481 gtggattgtc cgcgtatatt gctttactta aaatttcttg aagcttccct tttttcttcc
      541 tcatgtatta ttaattcttc ttttctatta aaatattaat acaataagca actcatgcta
      601 ctagtataaa aatttaaagt accaagttaa taataaagta atggagttat caaaaatatt
      661 tgaacatcct attcttattt acgatgataa atgttcatcg tgttataaat ttgctctttt
      721 gattcatagt ctttctaatg gttggataag aattgaaggt cattattatt caagcaatat
      781 tcacctaatt aataaaatac tttttccagc taattatgat cccaaaaaaa tgttttggtt
      841 agttaataaa aaaggagctt ggggagcacg ttctgcttta atacctttaa ttgtagaggt
      901 tattcgtgga agacttaact ctcattttgt aattaaaaga aactctacaa tggataatac
      961 atatagttgt aggttagatc ctaattctgt ctgttcagat tacttgaata tatttaagag
     1021 aattctagga gtattacgaa atactgggaa atttcctttt aattaaaaat atccctataa
     1081 acacttatcc tgcgcttgtc tgcctattat agtcgtcttc aatcctaatt atatcatttt
     1141 catcaaattt tccatatgat atttctaata ttatacaatc ctcttctaat gctgttatcc
     1201 tatgttttgc accacaaggg attgttatat tttcattttc tttaactaca agttctttct
     1261 tatcaatatc tatcttagct tttcctttta caattttcca aaattcactt ctatgttgat
     1321 ggtattggag actcaatctg gaattcgcat taacataaat caatttgaca gtacaattct
     1381 cattctcatg gaatttttca aattttcccc atgggcgact ttctgtataa attcccattt
     1441 agtattaaat gattatatct aatcatataa taattactaa tgaatattca acattcattc
     1501 aataagttat aatgttgtat tgggaatgta taaaagatca ttaaaattaa aattaataat
     1561 tatatgtctg aaatacattt tactgaagga aatttaatta ttctttactc tatccataaa
     1621 aattcctctt tacaaatacc taatcatatt cttgaaagaa ttgaacattg taataggctg
     1681 tatcagatta ttttaaaatc aaaaccagat tctaataaaa ctattatatt agtagttgct
     1741 gaacctcatt attctgaatt aataaaaaaa gaattgatca atagacagat aaaggaagaa
     1801 attataataa ttgattcaga ttcaaataaa gtatctcata cctttgataa tcttaaagaa
     1861 ttgcttaaac acaggattaa tccaccagtc gtatattttg taggttcggt ttggctaaaa
     1921 gatgtatttg attcaattgt taaatcgcag tttaaagaat acaagattca atttgaaggc
     1981 gcattagacc atcgacctgt catggaagtt gaacacgata aatcccttga aactcccaaa
     2041 aaaggaaaag aacacttcaa gcaggtaata aaaaataaag catttgatat gttattaaat
     2101 tatatttttc cagaagacca caaataataa ttatctttca aaaataagaa ctaaaaaaga
     2161 agttttagtg aaaaatacac tattaataaa atcggattag attttatcga gctaacttgg
     2221 attctttagt aaacaagtcc gctacttgtt ctttagtaaa agcaaaattc catacttgga
     2281 tatcatctaa ttgtcctgta taattaccat ttatctttcc cttttcagct aaagaatttg
     2341 ctcctaatct tatggtctgt tttcctgtat tatcaggtgt tattcctacc tttgttgagt
     2401 tagttgcgat ctcaaggccg tctatatata actttaacaa atgtaatttt tcgtcaaaag
     2461 ttagaattgc attgtgccag acgccatcag cgtaggagcc ctgagaagta atataattat
     2521 cctctccact ggaggtctca aatccgcctc caacttgttc tctattgtta agccatattc
     2581 catagttaag attaaatcca gccctgtcac ttccaaatcc ccctttattt agtaaaaaag
     2641 cattatttcc tgaagtgacg ttcattacag tattaaacca tacagaaact gtaaaaccat
     2701 ttagattaat atcacgacta tttacaacat ctgcataatt aacaccagaa aagtctatta
     2761 tcttggagtt cttaacaaca ggggatttat tctcttgagc aaatatctct atattattta
     2821 atatactatt tttgtagttt tctgtcaatc caaagtataa caataaaata acaaaactag
     2881 aacctatgag gcaagaaatt cttctattca tttatagttt tataattttc acaatttaaa
     2941 tattttttct ttttatctca tagaaataat aaaatagaaa tttaaaaaag tcagattttt
     3001 aggtttaagc tttaccaact aatttttttt ggagatgtaa gttgcttttc taattcatct
     3061 aatctttgaa ctgaaccggg attcttacgc aattccgaaa aagagtcatc atttctaatt
     3121 ctttgcatta ccgcaattac ctcttcttct gttacaccat gttttttatc atattctatt
     3181 tgttttgcac aattagacat agtactatat tttttaaaat tgcgcttctc aaatgaagtc
     3241 gcagttggag ataacccttt tacataacga acaattactt tttcaacatg ttctaaatgc
     3301 cattctctca tagcaaaact attaggtttc ataaacatac ccacttctat ttttgccaaa
     3361 aaggatattt aaattaatat acaaattatg ttatgaaatc gttaaattta taatatatat
     3421 aaagtttttt tatatgttaa tatactttat caaaattttt agaaatttat tccgataaat
     3481 attctgataa tttaaggttt tcttctccag gtatctttgc aatttcgaac ctttctttga
     3541 cttttaataa cgtagccgtt gcatcaattc ctaattttgt ggtaagtaaa ttcacttggt
     3601 cactagaagg atcaaggcta gatccttttg cattctgaat aataatcagg tctttatctg
     3661 cttgacatct cgttgatact gcgtattcta cttcgacagg atttgcaggg ttaatatctt
     3721 catcaactac tattgcaatt ttgagtgagg gatgggctgc aaatgctgca agtaaagcat
     3781 tttttgactc tccttctact cttttcttta tctgtattac tgcggttagc caattgcttc
     3841 ctccgtctgt cagcaatact tgcgatgtac tgggaacaac attttttact tgatctagca
     3901 tctttgactc cacaggcaat cccatcaata gacgatgttc agaataacca ggaagtatat
     3961 catggaaaat agtattattt ctaaatctta ttttttctaa ttcaaatata ggttgctgtc
     4021 ttctaaaatc atatgttttt aacatctcta ccatccattc ttcatgggtt ttatcacaca
     4081 atatttttcc ttctaaaact atttctgaca atgagggaat aagaagctgt gaaaacttgt
     4141 taatacccaa ttgcagattt ccatttagta gtgtatttgc tatatgcatt tcatctatgc
     4201 catatgatgc ttggtaagct gctgctatgt taattgcagg atgaactcct atcacaattg
     4261 cgaccttcaa atcttcccca tgatctttag catacataaa acatttatgt aaatgtcttc
     4321 cctctaccat tcttattgcc atgtttttat catctaattt taacaaccga tgggtagaag
     4381 aattctgatt tcctttttct tgatcttttg catacacaat tgatgaagta atatatggtc
     4441 cagcatcatt ttcaaaatgt gtaacaatag gaagatcaga taatttattt gatgaatttt
     4501 gataatacgg aatgtcattt actgttttta ctttaatatt catattgttg atagatttgt
     4561 taatgattgt atggatgtcg tttttttctt ttgatcctat tgccaaggca aatctttttc
     4621 ttgttccaat aagattacta attactggaa tagttttatc ttcgacattt tcaaatatta
     4681 cagctttatt accatcaagt ttactgacta ttgctggtaa ttcaaatgta gtattaacat
     4741 tctttttaat tctaatgatc tcatcttcat tttcaagtaa ttctaaaaat tttcttaggt
     4801 cgttatgaat atttgtcgtg ttagaattat ctttttgcat ctttttcagc ctgtgtacct
     4861 atcatattta gtatctcacc cataattgtt ttcatactct taacatctaa tggtgtttta
     4921 atatggagag aaactaaaca aattccattt atcattaatg atattctttg agcaatggag
     4981 ttaatgaaga ctgaatcaaa tttgctgggt attattatag ctgatgttac tttatttgat
     5041 agactagacg ccattgtaac agatccgatt ctattagaat tttctgatat tatgataaaa
     5101 catccgtttt caaaaaaaat tgatttgata tgaacaattc ttttatcatc tatctgaata
     5161 tctttgacta acatatcgcc tttatcttcc attttttatt attattaata ccaatatcat
     5221 ttttattaaa ttaagttaac ggtaattgtt gaaaaactta agtattttta tagaatttat
     5281 tttgttattc aaattattcc tactgtaaat tagagaatct ttaattgttt gtatacaaat
     5341 acttaaaata taatttatgc caaaatatta ttaaataatt attgttatct ttataataaa
     5401 aatttaaagt taatatgtaa aatccgtcta aaacattata tcaattttcg aatgtaaatt
     5461 ataaataaat tttccatgct ttaatcaata ttatattaat tatgatctag cttgggatca
     5521 aaaatctttc catttctaaa agcaattagt ctagctgatg atttatcttt aagtattctt
     5581 ctattacttt cattattttg gatagatgag gttccatgct catttactat ctcaacatga
     5641 actaaatctt tgaaatttga ttttattaaa aatgcaatac ttctagccat attgatatta
     5701 ccgtctccta ttcttacaat ctttttcttt gcatttatct ctgccagtat tttgaaaata
     5761 aaattcaaag ttgattcgat tgtagataat actacacttt ctatttcatc atataaatag
     5821 aaaatcgata tgcctattct atttcctgga tcaataccaa ttgttaattg atcataagta
     5881 tgagatttta ttaatttcct taatattttt gcctttatta ttacaggatt ttcatttaat
     5941 tctgaatcca ctattatatt ttttgtcttg aatataggcg attcttcctt ggatgtgatt
     6001 attattgttg aataagagtt taaagcttct gatggtgata actcctcata atttaactga
     6061 agtagtttga gggtattaat aattttataa tatgatttac cattaagtgt ggctacggta
     6121 attttatggt ttattgcttc cctcattaag ggtaattcaa caaagtttct ttatcactaa
     6181 ttagtattaa atgttatcta ttctatgaat taattattta tttttgtaat agataaagca
     6241 gtaaaatcgc ttatttgatc ctaaaattta attatttctt ttttatatcg attttattta
     6301 attatcaatc tctgatagct gaagatgtag agatatagtt tgaattctag tctcgttcat
     6361 tcttcaaatc tttgatattg ttataatcgt tatggcagga atgatagaag aacaaccttt
     6421 gttcttatta gaagctattg atttagcaat ataattacta attatatctc caattagttg
     6481 gataataagc acaatataga aattaattta aatttacact ttatacttag acattttaaa
     6541 ataaaaattg ataattatat attaacatca ttaacattaa aggatttaac aagaccatat
     6601 tagaattcta ttacccactg taaacaacct gaggtaatta aatagactag tagcattgca
     6661 aaaaccatga atgacaaaat aaatgtcata tttagtgtct acagtaaacg cttttataaa
     6721 aaaggtagaa atcatttaaa cgatattgat ttagaaaaca atctttttac aaaatcaaaa
     6781 taactagaat atttccataa tataaaattc taaccaaatg aaatttagtt ttataagaaa
     6841 tgatagtaat aaaaaaatat tatcagattt aattataata agttctgtaa ttggatagtt
     6901 taatttcttt aataaataat catttagcgg attattactt cggttaaagt atctgatgat
     6961 tacaactaaa aagattgtac tatacctgat aaatagtgca aattctatac aaaaattaag
     7021 tgttagtata attaatgact aattctataa caataaaata tgttaatata atacatagga
     7081 tagttcatta aaacacaaaa tattaaaata gaataggttt acaacataac tatattagta
     7141 attattatta ataattatta tttaattatt gttaatatca ataacttatt catttactat
     7201 ttttattaaa ctaattattt tatataatat catacaatac aaacattaat tatatacatc
     7261 atgttttttg tgtataccca ataaaaaata attactataa ataatctatt tgtggatgtt
     7321 attcttgtat tatgtaaact ctcattattt attgaattca tcatatttat caaaatataa
     7381 aatatcctat ttttactcct cttctaaaat ttttataaaa tgctctttat cttttatttc
     7441 ccttttagat ttgaaacaac tatattttcg tttaattact ctttactaga taatcaattt
     7501 tggagtccga tataaaatcc caaaaatcgc aagtatatca atacgaatca attgtaaatg
     7561 atagcctttt atggatagat ataagaaatc caacccgtga aaagatgaat aaccttggga
     7621 gacgatattc ttttcatgaa ttaaatattg aggactgtct ctcaaaaact caaataccaa
     7681 agatcgacaa atatgataat catgtttttg taattttaca ctttccaact ggaagtactg
     7741 atgataaaat accaagagtt actcaattat ctatttttat tggaacaaat tatttaatta
     7801 ctgtacacca agatgatctt aaacctttag tagaaatgtt tgatttgtgt aaaaaagaaa
     7861 aaaatcaaga gtatcgtaaa gtgatgatgg gaaaatcttc aggctattta ttgcatacta
     7921 tcatagatgc attggtagat gatctttttc atattttaat gaaagtaata ggaaatctag
     7981 acgatattga agacgcagtt tatgactcga aaataggtat tgccagagaa atttctcata
     8041 ttagaagaga aattactact atcagacgtg tatcaatacc cttaaagaga ataattgtaa
     8101 gccttacaag agatatccag aagatttcag aagaagatct tacgccttat tttgatgata
     8161 ttaaagatca tattgataaa gttttagata ccttggaaga ggcaaaagaa actatagaaa
     8221 tttataaaga tactgacttt atgttaagta cagaaaaaac taataaaatt atagctatgt
     8281 tgacagtagt ttttacatta tcaattccaa taactgtaat tagtacaata tacggtatga
     8341 acatttctct tccaggatct gatctaaatc gattccaatt ccttggagaa tatactactt
     8401 tgataatagt aatgttaata tcaattataa ttacaggtat aatgtttata tcatttcgta
     8461 gattaggatg gctggctagg agaatttagt tttttataac ctatttctgt aaatatgttg
     8521 tctcttgaat tactaaggac aaaaataact aataaaggtc aaagaattac tcctttattt
     8581 tgtttagtct ctgctgataa tctttcaata gcccaaaaac taataacaga atttgaatct
     8641 tcatataaca aaaaagagac aaaaggagat ttacaaaaaa gattattttt gtatgaaaat
     8701 agttacagtg attttaagct aattagaggt cttattacac tattagaaag acgatgtgta
     8761 ttcaaaatta atcaattcat tcccacaggt tataagaacc attttttttc aactcaatta
     8821 ccatcttcat tttcgttacg taaggtttta tttgaggaat cttcaagagg tggattgtct
     8881 ttagataata ctaaacgaga caaaattttt cagcatgttg cttcaaaatt aggtatagac
     8941 actaattatt tggaaaaatt aatgtggcta gatcaagaag attatctaat tctcgaaagc
     9001 tttagtgtaa ttaaacctat acatttacta gggatatata atttatcaat tttacagaca
     9061 ctgctattta attctgtcac ctttgaattc actataaaag gaggaacaaa ctggaaacaa
     9121 gtattaagaa caatcaaaaa atttggacta atgtataatc ttcggaaaat tcaaaagaat
     9181 tcagacaaca tatttcctaa agaaatacaa tataatcaaa ttgaacaaaa tattgtagat
     9241 ggagatgatt ttaaatcata tttcaatgag agtatcgttt gctcaataga tggaccttta
     9301 agtatattta aattaactaa taaatatggt attttgatag caaaagtaat tcctaaaatt
     9361 atttcagctt cgaaatgggg tattaaagca tcaataatta aaaatagtct tgcaggaaga
     9421 aaactttacg attttgatct ttcatcagat agcgaggtat atttctttaa tcctattaac
     9481 gaccgatttt ataataatta cccatttgaa gactctaata gcattaatct tgattttgat
     9541 agttctgttg aggcaaaatt tgctatacaa tttgaaaaat ttcgtactgg ctggaaactg
     9601 gttagagaac ctgatcctct aatattgcca ggtggaagag ccttcatagc tgattttctt
     9661 tttgaaagat atggcaaaaa aatatatttt gaaataatag gattctggac accacaatat
     9721 ttagagagaa aatttaaaaa aatttacgaa atcataaaat taagcaataa taaatacgat
     9781 cttttaatag caataaacga aaataattta gtttcggaaa gtggtgaaat gaaaaaactc
     9841 ttatcaggtt ctattttaga ttacgataaa atcataatat ataaaaaaga ttcaattccc
     9901 ataaaaaaga tccttttcta tctgaaatct atagatagta aaataatgaa tcaaaatctt
     9961 gaaacctaca gatctgcaat gactgaatat attgtagatc tcttaaataa gaaccaagaa
    10021 ataattgatt tagaagaaat atctaaaact tatggtgttt ccattaattc tatatctaat
    10081 attatttcaa acctagatac agataatcat atcaaaaagt atataattca aaattcttta
    10141 ttaatttctg agagaaaatt aaataaaata aaagatagaa ttggtgatat tgataattta
    10201 ataaaaatac aaaagatttt tgaaaataat aatatcccta tccaatatac tatcgatatt
    10261 cttaaatatt taggatatga aattgtctgg aagggaatgg attcgtcaaa tattataata
    10321 aaacgaaaaa catgagatta taatttatat tatattcata tgttaaaagc gtttataaaa
    10381 agacataacc attgaaatat ttaaatttat gataagattt agtaataata gtgtatatga
    10441 acaatattaa aaatattaaa attaataata atacctggtt acaaaatatt tctggaaact
    10501 ggcgcgatat agaatgtcct tgtggaagtt gttatcattt taatggaagt cttgaagaat
    10561 tatttaattg ggaaaatata catttaagac atatggcatc agattatgat aatcatgcaa
    10621 tgatatcttt ttatcctgac gatatggtta ttttttatca ttctaaaact ggatatgtta
    10681 ttgaaagaag aaaacataaa acatatgaac taaatgaaat taaaaatgca tggattcacg
    10741 gaaaaataac aatcacaggt gcataaattc tctaatgatg cttacgatac tatttaaata
    10801 taatattgtt tgttcgttaa atcagtcaga tatactaaac tatttccttt aaatcatatc
    10861 tgtatattag aattatggaa atctatagat gtaactttta ctgcttttaa gtattttaat
    10921 ttcaa
//
LOCUS       DASVEV010000197         7873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1640359, whole genome shotgun sequence.
ACCESSION   DASVEV010000197 DASVEV010000000
VERSION     DASVEV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640359"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF222_08900"
     CDS             <1..555
                     /locus_tag="VF222_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7142583.1"
                     /translation="EALKIARNKAKSSEIGKAIENSIIKSGYIPIKNLSGHSLERYTI
                     HAGKSIPNINSYGSFLLESGQAYAIEPFVTLKNGIGIVYEGKVANIFAIISRKPTKNK
                     EVDNFLNDIWERYRSLPFALRWLIDIYDEKHARYLLDNLKKKKNIHAYPTLVEGNKKM
                     VAQSEHTIIPHETFTEVITSCNNS"
     gene            630..703
                     /locus_tag="VF222_08905"
     tRNA            630..703
                     /locus_tag="VF222_08905"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Pro,seq:tgg)
     gene            1884..2120
                     /locus_tag="VF222_08910"
     CDS             1884..2120
                     /locus_tag="VF222_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142584.1"
                     /translation="MKIIKLLKNQENTYEVILFNIDLTELSAKETFEDLYTVNFYLQS
                     MQKKLNFHESLIIIHDKFNNTVELVKNNGEIFIE"
     gene            2190..2369
                     /locus_tag="VF222_08915"
     CDS             2190..2369
                     /locus_tag="VF222_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142585.1"
                     /translation="MGKIAKKGRLFLGKRIGPKSARRARKPLSIDWITGNIPNRHYVN
                     KRRNRKIISRKNNLS"
     gene            2460..4169
                     /locus_tag="VF222_08920"
     CDS             2460..4169
                     /locus_tag="VF222_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX7142586.1"
                     /translation="MKLISYFPLKKIRNNQEKVLDEIEEAIKSNIKYFFLEAPTGFGK
                     SPIAITLASYLGSSHICTSTKDLQNQYSKDFHFIKEAKGKRNFRCILKENEGVQETCE
                     YGPCQKDEDFDCMYKTRFSDYKINGLGTKFEHVDIGQFEKKKYLEKYDQQIRFENLEW
                     EPCRYFHQKWISTKSSHAIYNYKYFLSDLYFSNIISKRELLVFDEVHNIESEISDFKS
                     FIINSDNITRLFPKLQLPNRKEEEIETWIEFCEVYRNTLLDFMEDFELAMENSTPKEP
                     FTEKNLIDCINREKNLNFVLNDMKTNKNNWLVTNIDRTSRDLIRKITLTPLDISNYFK
                     EILDLSNYGLFMSATILNKDYLCKISGLSKDDIKFIRIKHSDFPKENRPIHLMNIAWL
                     NSNNILDNLPVIAKTVNNIMSIHKNEKGIIHTTSYFQLEFIKNNLNKENLQRLIESGP
                     NTDRIETLQRHSQNPKPTVLISPSFYQGLDLKDDLSRFQIIIKIPYPDLSDKKVAALR
                     KKDMNWYLWNTIVRLTQTYGRSIRSKEDHASTYILDSNINYLLRNVNDMFPSWFTEAI
                     INR"
     gene            5195..5425
                     /locus_tag="VF222_08925"
     CDS             5195..5425
                     /locus_tag="VF222_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142587.1"
                     /translation="MTDSGIKLNPDKMIKEELYHCIYENRIFLFFKDNDDVLHCYEVS
                     DIDTINKIKQTPENIDDILNNLAKDSSANIQD"
     gene            5465..5779
                     /locus_tag="VF222_08930"
     CDS             5465..5779
                     /locus_tag="VF222_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX7142588.1"
                     /translation="MSTFNENTERKPKSSNEIFIGKKPLMTYVTATLVQLANEPTVVI
                     KARGRSIPMAVDVAQIIVKRMNTLGYKVASVKIGSEAVKSQDGNTRNVSTIEVAVSRN
                     SS"
     gene            complement(5817..6485)
                     /locus_tag="VF222_08935"
     CDS             complement(5817..6485)
                     /locus_tag="VF222_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142589.1"
                     /translation="MYKIFPLFNLLFFILFIGDEIGKNVLVSGEIFNGYTSKLVEKSI
                     IKSPVTNTSFNKELSGLYNEERIDNNKTQLQQPVVMVTTNIFSCIIENINSEKLCRTL
                     YDCNEEPCKKIDLRSNLVVYGKEPIYSIITSNSGINSKEIKFQPGEYEILLKNNDFIS
                     SSFCHEIVIDGLSFHEFTVGIHLSPDNRSILCMNISDGCYGIIKSGETRICEINKVLI
                     KNIF"
     gene            7123..>7873
                     /locus_tag="VF222_08940"
     CDS             7123..>7873
                     /locus_tag="VF222_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7142590.1"
                     /translation="MEIYGFKSFGFKNTIINFNNGLVAITGPNGSGKSNVLDAIMFAL
                     GENSPKALRVDKFQSLFHDTQNKSHKLVRVSITFDNQNRGVPIDSDNVTLTREMEGSV
                     GESQYHLNGKKVTKSTIIELLEIVVAVPNKLNIVQQGMITRISELNSEERRKIIEDIV
                     GLSYFDEKKEESLKQLDESDRRLDIALARMNEIRKRIDELEVERNEQLRFQHIEKDIK
                     KYKAIKISNESRSIRRNIMTNNQELETKRIKY"
BASE COUNT         3086 a          989 c         1120 g         2678 t
ORIGIN      
        1 gaagctttaa aaattgcaag aaacaaagca aaatcaagcg agattggtaa agcaatagaa
       61 aactcaataa ttaaaagtgg ttatattccc attaaaaatc taagtggtca ttctctagaa
      121 cgctatacga ttcatgctgg aaaatctatc ccaaatatca attcctatgg atcatttctt
      181 ttagaatctg gtcaagctta tgctattgaa ccatttgtaa ctttgaaaaa tggtataggc
      241 atagtatacg aaggtaaggt tgccaatatt ttcgcaataa tatcaagaaa gcccactaag
      301 aataaggaag tcgataattt tttaaacgat atttgggaga gatacagatc tctaccattt
      361 gctttaagat ggttaattga tatttatgat gaaaaacatg caagatattt gttagataat
      421 cttaaaaaga aaaagaacat tcatgcttat cccacgcttg ttgagggaaa caaaaaaatg
      481 gttgcgcaat ctgaacatac tattatacct catgaaacct ttacagaagt tataacgagt
      541 tgtaacaata gttagaaaag aatcctattt atggcggaat cttcctaagc ttaaaattaa
      601 acctttaaaa atgtatagta agacgttagg gggtcgttgt ctagcttggt atgatgccag
      661 ccttgggcgc tggagttcgc cggttcaaat ccggccgacc ccattgattg ttaactcttt
      721 tagtttacat aggataaatt ttgattcaac ctttcttcta ttaaaaatta aacaagaaaa
      781 atattatagt tagacttttg tcttacagtg tcattttaaa atactgtcta aaattatcct
      841 atgatgttat gctagtgaat tcaataagtt ctatgatttg gaatttgatg gaattattta
      901 aagaaataat aataaaagga tttttatata aatatttata atttaatcaa aaaaaattat
      961 aattttaata gtgttcagaa taagtgtacc gatttcctaa tcaaagataa ttgcatctat
     1021 agttatttga accggataat tttcaaatat tggtttatta tgtatcttaa aatcttttat
     1081 aggagactaa atctttatag tcttttaaat atgatatatt gcaaaattaa agttgataac
     1141 ttattatcat taaaatgacc tccatctaag tactgtgcga tatacaaaca ttcccctcat
     1201 aacacattcc aaagtctctg tagcaaaatc ctagtttgta atctttaaaa taaatgtgtt
     1261 tgaggtaatt tgagaaatta gattctatta aaactataaa tgatggttag atataataat
     1321 aatattgcct ttactttatt acaattgtga atgctacttt ttattctaat gctgttaaat
     1381 aaataaaaat aatttaatct attgtaacgt caataaaaga aaatacaaca atgaaattaa
     1441 ctcctaaaat gaagttttca aaatatatta gctatataga tacaaggcta attgattaga
     1501 ggtttatatg taactgttta tattattaaa aaatatctga gttcattgta aataaattgt
     1561 taaatcgtca cataatacat aaagttagga tatttaaagg ttattcaaat ttgattgtaa
     1621 atgctattta tcctcctgac aacacatcat ggtcccaatt attttgttga taattatcaa
     1681 tcagttcatt tagtaaatca tcataatcca tatttctttt tcttttattc attaaatcac
     1741 ttaacaatga attgaatctc ctaaaagtga attcattgat tgctagattt gccacaaata
     1801 ttcttttttt ttttaaaata tgtttgtttt gcatttattt taaattgaga agacttttaa
     1861 caaaaagaaa aaaataagta gatatgaaaa taataaaatt actaaaaaat caagaaaata
     1921 catatgaagt tatactcttt aacatagatc taactgaatt atctgccaaa gagacttttg
     1981 aggatttgta tactgtgaat ttttatttac aaagcatgca aaaaaaatta aattttcatg
     2041 aaagcttgat aattatacat gataaattta acaatactgt ggaattagta aaaaataatg
     2101 gagaaatttt catagaataa ttgttaattt aattgtgaag ttatcaaaca ataaactaat
     2161 tagtcttctt gaacaatgtt agttagacaa tgggaaaaat agcaaaaaag ggtcgacttt
     2221 ttttaggaaa aagaattggt ccgaagtcag cacggagagc acgaaaaccg ttaagtatag
     2281 attggattac tggaaatatt cctaataggc actacgtaaa caagagaaga aatagaaaaa
     2341 tcattagtcg aaaaaataat ctcagttaat gcatttagat aaattaggat gtatgttcac
     2401 tcgaaagacc aaaataaaat acaacgaaaa aaaatcaata tggatttttg gatgacatta
     2461 tgaaattaat aagctatttc cctttaaaaa aaataagaaa taatcaagaa aaagtattgg
     2521 atgagattga agaagctata aaaagtaata taaaatattt ttttcttgag gctccgactg
     2581 gctttggtaa aagtcctatt gcgataactt tggccagcta tttaggaagc tctcatatat
     2641 gcacatcaac taaagatctt caaaatcagt attccaaaga ttttcatttc attaaagaag
     2701 caaaaggtaa aagaaatttc cgttgtatat taaaagaaaa tgaaggagtc caggaaacat
     2761 gtgaatatgg cccctgccag aaagatgagg atttcgattg catgtataaa acaagattct
     2821 ctgattataa gataaatggc ttaggaacaa aatttgagca tgtagacatt ggacaatttg
     2881 aaaaaaagaa ataccttgaa aaatatgatc aacaaatacg atttgaaaat ttggaatggg
     2941 aaccgtgtag atatttccat caaaaatgga tatctactaa atcttctcat gccatttata
     3001 attataagta ctttctttca gatctttatt tttctaatat aatttcaaaa agagaattat
     3061 tagtatttga tgaagtacat aatattgaat cagaaatttc tgattttaag agttttataa
     3121 ttaatagtga taatattaca cgactatttc cgaaacttca attaccaaat agaaaggaag
     3181 aggaaataga aacatggatt gaattttgtg aagtttatag aaacacgtta ctagatttta
     3241 tggaagactt tgaactggct atggaaaata gtactcccaa agaaccattt acggaaaaaa
     3301 atttaattga ttgtattaat agggaaaaaa accttaactt tgtccttaat gatatgaaaa
     3361 ctaataagaa taattggctg gtcacaaata tagaccgtac atcaagagat ttgattagaa
     3421 agataactct tactcctttg gatatttcaa attacttcaa agaaattctc gatttaagta
     3481 attatggatt atttatgagt gctactattt taaataaaga ctatttatgt aaaatatcag
     3541 gattatcaaa agatgatatt aagtttattc gaataaaaca ctcagatttc ccaaaagaga
     3601 atagacctat tcatttaatg aatatcgcat ggttaaatag taataacata ttagataatc
     3661 tcccagttat tgctaagact gtcaataata taatgagtat acacaaaaat gaaaaaggga
     3721 ttattcacac cacatcctat tttcaattag aatttattaa aaataattta aataaggaaa
     3781 atttacaacg tcttatagaa agtggaccta acaccgatag aatagaaaca ttacagagac
     3841 attcacaaaa cccaaagcca acagttctca tctctccttc attttatcaa gggttagatt
     3901 taaaagatga tttgtcaagg tttcagatta taattaaaat tccttaccct gatttaagtg
     3961 acaaaaaggt tgctgcatta agaaaaaaag atatgaattg gtatttgtgg aatacaattg
     4021 ttagactaac tcagacttat ggacgtagta taagaagtaa agaagatcat gcatctacat
     4081 acattttgga tagtaatata aattatttat taagaaatgt aaatgatatg tttccatcct
     4141 ggtttaccga agcaataatt aacagatagt ataattatac tatcattaga gtaccttaac
     4201 aattgcaatt taattagatt tcagatataa tgcaatactg tgtatttatc ctcgtttttt
     4261 ttactttttg atactaacaa atcaatatgt tcaatattca aaataccata aaataaaact
     4321 taatttggat aaattaaatt ttaattttaa ttagacgcta aattgaggac aataatgctt
     4381 cacacttctt gtcctattct ttatgagtta tttagtatct ataaaaaata aaataaacag
     4441 tactaaataa caagtttttc gaatgaaatt ttattaatca atttaaagtc catacattgg
     4501 ggtttacata taattaatat ctgttgtatt ctcttttaat aatgtgatct tagttatttg
     4561 ttaatatttc gttgtgtatt tacagaacta ctatttattc ctaaaaatgg tttgtataac
     4621 tattgtccat aggaatttcc atatattgtc aaacaaatct atatttgttt agttaattgt
     4681 ttaaaggtag ttattccaaa ctagttgagt ttttttgttt aaccagatat gaaacgtata
     4741 tcttaaaccg gaaataattt tagcattaat cgatgtttag taaaaaatag tccattaagt
     4801 taatcttctc atgcgatctt tcgtaaaaga ttactataaa caatttcaat tggacaatgc
     4861 ccctttactt cataccgaag tgaatcttta acattcgagc aaaaattttt tttttaatac
     4921 tcataaaaat atctgtcaaa tgtaaataat tctcactaaa ctactttgtt catattttcg
     4981 gttacagatt ttattattta ttattaatga aataataaga tcatataata ttcattatca
     5041 attgatatgg tgatttttga gataatatat cttcatctaa agttaagata cattatattt
     5101 tatacaaaat ctttacaaat tcgataaact ttatctagat agattttttt attccaaatt
     5161 attattatta agctcattgg aaaatcctgt aattatgaca gatagtggaa taaagttaaa
     5221 ccccgacaaa atgataaagg aggaattata tcattgtatt tatgagaatc gtatatttct
     5281 tttttttaaa gataatgacg acgttcttca ttgctatgag gttagtgata ttgatacaat
     5341 taataaaata aaacaaactc ccgaaaatat tgatgatata ttaaataatt tagcaaaaga
     5401 ttcctctgct aatattcagg attaaaaaaa cattatatgc tactttccat gaatattaaa
     5461 attaatgtct acatttaatg aaaatacgga gcgtaaacca aaatctagca atgagatatt
     5521 tatcggaaaa aaaccactta tgacttatgt cacagcaaca ttggttcaac tggctaacga
     5581 accaacggtt gttataaaag caagaggaag aagcataccc atggccgtag atgttgcaca
     5641 gattattgta aagagaatga atacattggg ttataaggta gcatctgtaa aaattggttc
     5701 cgaagcagtt aaatctcaag atggaaatac gcgtaatgtc tcaacaattg aagtagctgt
     5761 ttccagaaat agctcttaaa ttgttctatt tttaacctat caggaatagt tccaagctaa
     5821 aatatatttt ttatcaaaac tttattgatt tcacatattc tagtttctcc tgattttatg
     5881 ataccgtaac aaccatcact gatgttcata caaaggattg atcggttatc cggagaaaga
     5941 tggattccta ctgtgaactc gtgaaatgaa agaccatcga ttacaatttc atgacaaaaa
     6001 cttgaggaaa tgaaatcatt attttttaac aaaatttcat actcaccagg ttggaacttg
     6061 atttctttag agtttatacc actattggaa gtaataatgg aatatattgg ttctttacca
     6121 tatacaacga gatttgacct gagatcgatt tttttacagg gttcttcatt acagtcatat
     6181 aaagtgcggc agagtttttc agaatttata ttttctatta tacagctaaa tatattagta
     6241 gtaaccatta caactggttg ttgtaattga gttttgttat tatcgattct ttcttcatta
     6301 tacaagccac ttaattcttt attaaatgat gtattagtaa caggactttt aataatagat
     6361 ttttctacta gtttcgatgt ataaccatta aaaatttcgc cagaaactaa tacattttta
     6421 ccaatttcgt cccctataaa aagtataaaa aataataaat taaataacgg aaatatttta
     6481 tacataaagc attcagattt tcaagatatt aattagtgat gaaattttta ttaggaaaat
     6541 tatacgtgta gaatgaagtc aatcaataag agaatacatt tttactttag atatttaatt
     6601 aacaaaagaa aaaaaaatta tttaaaaaat aaaaaaaata aatatcgatt ttgctgaggg
     6661 agtgcctcat attgaatttg atgtaaaata aagatcatat tatttttaac attcataaat
     6721 gtttctgaat caagatattg tggaacataa caccagacta attattaaag atagttcgag
     6781 attatataag aaactttata gcaaagaaaa tgacatactt gatatattgg tttctgcgat
     6841 tttatttttt tacttaccta tcactattat attaaccctt aggaattagt aaaataactc
     6901 aataaagcta aaatcttctt attaatcaca caaaaggatc ttatcaggat ttagaattct
     6961 ttagaaaaat tcattaaatt ataaaatgat tcaaatttta atcttacata ctgtaaaagg
     7021 atgagttaaa gttaaccacc taaaggctta acctaaaatt taataaacaa tataatatag
     7081 aatcagcttg ttagtctaat caggatggtt tttattaaaa aagtggaaat ttatggtttt
     7141 aaatcattcg gctttaaaaa tactattatt aactttaaca atggactggt agctattact
     7201 gggcctaatg gttcaggcaa aagtaatgtg ttagacgcaa tcatgtttgc actaggtgaa
     7261 aatagtccaa aagctttacg ggtcgacaag tttcaatctc tttttcatga tacccaaaat
     7321 aaatcacaca aattagtgcg tgtaagtatc acttttgaca atcaaaatag gggagttcca
     7381 atcgattctg ataatgtaac actaactaga gaaatggaag gttcagttgg agaaagtcag
     7441 tatcatttaa atggaaaaaa agtaacaaaa tctacaatta ttgagttatt agaaattgta
     7501 gttgcagttc ctaataaatt aaacattgta caacaaggaa tgataacaag gatatcggag
     7561 ttaaattccg aagaaaggcg aaaaattata gaagatattg ttggattatc ctattttgat
     7621 gaaaaaaaag aggaatcatt aaaacaatta gacgaatcag atagaagact cgatatagca
     7681 cttgcacgaa tgaatgaaat aagaaaaagg attgatgaat tagaagttga aagaaatgaa
     7741 caattaagat ttcagcatat agagaaggat atcaaaaaat acaaggccat aaaaatttca
     7801 aatgaaagta gaagtattag aagaaatatt atgactaata accaagaatt agaaacaaaa
     7861 agaataaaat ata
//
LOCUS       DASVEV010000198        23298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1445173, whole genome shotgun sequence.
ACCESSION   DASVEV010000198 DASVEV010000000
VERSION     DASVEV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 23298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23298
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445173"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF222_08945"
     CDS             <1..204
                     /locus_tag="VF222_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142591.1"
                     /translation="RGLRDEVDLGEVQEVGTEYVLTKKGTIDSDKFYIPKNLVERFDG
                     RTVWFKLTEEESHQYKEHKTHLI"
     gene            complement(559..1410)
                     /locus_tag="VF222_08950"
     CDS             complement(559..1410)
                     /locus_tag="VF222_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7142592.1"
                     /translation="MIQHKFGWTGVDVPVIGQGTWMIDGDSETEARAIRTLRLGLDLG
                     MSHIDTAEMYGDGHVEELVGQAIAGRRDEIFLVSKVLPSNASYEGTLKACKRSLKRLK
                     TDWLDLYLLHWRGSYPLSETMRAMEKLVTEGLVKYIGVSNFDVEDLMEAEQALQTERV
                     ACNQVLYNLRDRGIERRLLPYCSKKKIAVVGYAPFGHGNFPSPPSREGKLLVEIGRRH
                     NRTPKQVALNFLTRHPSIFTIPKTTRPERVKENSGGVGWQLIEDEVNVIDREFPVPSP
                     GTPLGVI"
     gene            1701..1991
                     /locus_tag="VF222_08955"
     CDS             1701..1991
                     /locus_tag="VF222_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142593.1"
                     /translation="MKGYKNNPKSGNNGKLMRLLDYALTTFIIPFVILLMKLLAYAII
                     TFVIPFILNIIKKKLSDQQRLLCNKCHGTLEVIDKEGNLYCKNCKMIKMDRH"
     gene            2681..2890
                     /locus_tag="VF222_08960"
     CDS             2681..2890
                     /locus_tag="VF222_08960"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX7142594.1"
                     /translation="MIKLSCKESGLDCDYIVEGKTEDEVLERISEHAINIHHMREEDI
                     YEEHVPVAFLCHAIGKMTSNHNQQN"
     gene            2990..3646
                     /locus_tag="VF222_08965"
     CDS             2990..3646
                     /locus_tag="VF222_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7142595.1"
                     /translation="MKVLSVNVGFPRKVLFNGQIITTAIFKDPVKGPIMLRKLNLDGD
                     KQADLTVHGGLDKAVYSYPAEHYDYWRKQFPNTDLVWGMFGENFTTEGLLEDAVNVGD
                     QFHIGSAKLVATQPRMPCYKLGVRFGRMDVIRRFMASGRPGIYFKVSTEGEVQTGDKI
                     KIIRRDKNNVTVKDIVSLYIARNDIDNIETMKRATKIRDLPEGWRYEFQQKIEQLKHK
                     "
     gene            complement(3728..4639)
                     /locus_tag="VF222_08970"
     CDS             complement(3728..4639)
                     /locus_tag="VF222_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142596.1"
                     /translation="MNKSQNKHMTKYKTVKLNGLDIFYREAGPKNAPTILLLHGFPTS
                     SHMFRNLIQKLSDEFHLVAPDYPGYGNSSMPPVDEFDYTFENMAKIISKFIDTIGLEK
                     YSLYMQDYGAPIGFRLFSMHPEKVQAFIIQNGNAYDEGLEEFWNPIKDWWNNNTPENE
                     KKLHYLVTAETTKWQYINGTRNPESISPDNWIIDQAGLDRPGNAAIQLAMLYDYKTNP
                     PLYSQWQENFRKYQPPTLIAWGKNDYIFPESGAHPYKRDLKDVETYILDTGHFALEED
                     YDVIAEKISGFFSARPQIKAVNKDIKA"
     gene            5246..5506
                     /locus_tag="VF222_08975"
     CDS             5246..5506
                     /locus_tag="VF222_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142597.1"
                     /translation="MDENLIERKKRLEKELDSKVSNDYREFINKSNNFLQQSRENTKK
                     YYQDLLSKELDSMENAIKSGSTLQAMSHRLTANVYESILQNT"
     gene            5826..6062
                     /locus_tag="VF222_08980"
     CDS             5826..6062
                     /locus_tag="VF222_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7142598.1"
                     /translation="MNTLISSMTHHIAKITEIVGSSEKGWQEAAQVALDEAKKTIREI
                     TGLEVVDKTATVDPNTGSITKYKTTIKIAFGVER"
     gene            complement(6264..6524)
                     /locus_tag="VF222_08985"
     CDS             complement(6264..6524)
                     /locus_tag="VF222_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7142599.1"
                     /translation="MDVTENDVLVTNGVSEALDMTLASVVSPGDEILMPGPHYPPYAS
                     YAKFYGGKPVEFKIHPDGKPDIDDLKSKINSKTSAICLAHQC"
     gene            complement(6511..6759)
                     /locus_tag="VF222_08990"
     CDS             complement(6511..6759)
                     /locus_tag="VF222_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142600.1"
                     /translation="MNMLLEISLRRQEHMKKKNGKNVIYLNIGDPVQYDFPTPDHIKN
                     ALIKAVQSDENFYTASEGLPELRQAIVEKEKGKGFGCN"
     gene            complement(7735..7908)
                     /locus_tag="VF222_08995"
     CDS             complement(7735..7908)
                     /locus_tag="VF222_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142601.1"
                     /translation="MNHVLKKRNRFRGKTENEKLYNDNMVLASELKEDIQIIINSHEK
                     QFVNKKADKDESD"
     gene            8067..8270
                     /locus_tag="VF222_09000"
     CDS             8067..8270
                     /locus_tag="VF222_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142602.1"
                     /translation="MKGLESMKHIVNSRINRLEYELKIAMEIEYSSKIVYQLEMLECC
                     KVIINVDLLRRTRKEENGISPFF"
     gene            complement(8720..8890)
                     /locus_tag="VF222_09005"
     CDS             complement(8720..8890)
                     /locus_tag="VF222_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142603.1"
                     /translation="MHKGKYSKKEYYFSYLQSSKNTTTVITPSPNTETTKPTFYKNPI
                     QIIQNQRWITHW"
     gene            complement(8965..9153)
                     /locus_tag="VF222_09010"
     CDS             complement(8965..9153)
                     /locus_tag="VF222_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142604.1"
                     /translation="MRCQNSTDNNIRCKRTASKIAIVKNIIEKQSKFWICDDCFNEKM
                     YNNPLFKDHLIQVMEVTR"
     gene            9795..10115
                     /locus_tag="VF222_09015"
     CDS             9795..10115
                     /locus_tag="VF222_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142605.1"
                     /translation="MPILPKTKSVYDSSIFEIKNNNLEEGIECFSHWLSIEEGNSEDS
                     NKNVQLTLIVFEKKNNKLLLHDNLHNSDIEVVFTDGVPFCNLCRTDDCVHAGFAICAK
                     QNHC"
     gene            complement(10360..11220)
                     /locus_tag="VF222_09020"
     CDS             complement(10360..11220)
                     /locus_tag="VF222_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142606.1"
                     /translation="MNQLELKKLFKSSVKNGHINNNHLLPFLNSQTHEIKIKEEGYFR
                     NFDLVIALINKESKQNKTNYSAKQMKNEYFDLEYDIQHNILMRSQQFTEFSKQEKCKI
                     ENITFIPVEIKSDNDVLDERLPNQILNAILTFGRSYLVIDKKYILNRNLQILKLLPTT
                     IIGYSGKDDYFEILSIFNRCIMNGIFNIPKRSFMKMLINNNITNKISDMVRIYHSLIT
                     LEQINQKLVFNYLFRAEENLDIDFLLNEEIDFLHQFCNFSRLPSEKTNTKQIKRLIKN
                     SRNYLITDFL"
     gene            12977..13270
                     /locus_tag="VF222_09025"
     CDS             12977..13270
                     /locus_tag="VF222_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HEX7142607.1"
                     /translation="MNEAALKELVMPHEGEMFGRVIKLVGGDNVIVKCNDGKVRTCRI
                     RGKIKRRMWIRENDLVLLAPWDFQSEKADIIWRYIAAHSDKLEQEGHLKGLNK"
     gene            complement(13374..14198)
                     /gene="hisF"
                     /locus_tag="VF222_09030"
     CDS             complement(13374..14198)
                     /gene="hisF"
                     /locus_tag="VF222_09030"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7142608.1"
                     /translation="MTLAKRIIPCLDVDNGRVVKGINFKEVKDAGDPVEFAKRYSQQG
                     ADELVFLDITASEQKRDIIKNVVRNVARVIDIPFTVGGGIKKLEDARDILLSGADKVS
                     INTSAIKNPELITELMSIFGKQCIVVAIDVKKNYSNVDPKKTLKDNYEHKKKQNKHYW
                     FEVMIYGGKERTGIDAIEWAKKVEKLGAGEILLTSIDADGTEEGYDLELNRIICKELR
                     IPVIASGGCGKPIHMLEVFQKTKVDAALAASIFHYDKSTVKRVKQFLKRNNIHVRI"
     gene            complement(14201..14920)
                     /gene="hisA"
                     /locus_tag="VF222_09035"
     CDS             complement(14201..14920)
                     /gene="hisA"
                     /locus_tag="VF222_09035"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7142609.1"
                     /translation="MNIIPAIDILEGKVVRLVKGDLRNKIVYSDNPIEIAEKMESQGA
                     NIIHVVDLDATLTTGWNNNQNNTEIILKIIDSIKIPVQVAGGIRSIDAINRMFEKKAE
                     KIVIGTLAYKQPQVLQQLKDKIEKIVISIDQNKGIVMIDGWRQPSGFKVVEAIKLFMA
                     KGIKEFLLTSVDRDGTLNGPDLDTLSYASNINGAKIIASGGISSIEDIIRVKNIGCSA
                     VILGKSLYDGKIDVKKALIVV"
     gene            complement(14917..15537)
                     /gene="hisH"
                     /locus_tag="VF222_09040"
     CDS             complement(14917..15537)
                     /gene="hisH"
                     /locus_tag="VF222_09040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7142610.1"
                     /translation="MNDIAIFDYGAGNIYSLKCSLERNGATKVSVIKSLKSIHKHDGL
                     ILPGVGNFDPAIRSIEPYRDDFDNLFEKNFPILGICLGMEMLFNNSEEGKLEGLKILR
                     GRVIMLPRKKVKIPHMGWNNLEITEKACKILQGLKDGCWVYFVHSYRVISRDKKVIVA
                     NSRYGGNIPVVVEKNNLFGTQFHPEKSGINGAKIIKNFLAICGGKY"
     gene            complement(15540..16157)
                     /locus_tag="VF222_09045"
     CDS             complement(15540..16157)
                     /locus_tag="VF222_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX7142611.1"
                     /translation="MNSKPLDLDKSQNRISVKDRFTKETNVSVSVNIDGSGNVEIKTG
                     IPFIDHLISAFGKHSMVDIYLEAKSNDSISHHLIEDVAITLSQTLNDALSNRTQIRRY
                     GYAIIPMDEVMAISAIDLIKRQTYKINLGLTRKEIEGIAREDLEHFIESLLQNLNACT
                     HINIQYGENDHHKIEAAIKSFAIAFRMASCIDYQRNGIPSTKGVM"
     gene            complement(16171..17205)
                     /locus_tag="VF222_09050"
     CDS             complement(16171..17205)
                     /locus_tag="VF222_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7142612.1"
                     /translation="MYQKINNINFIHTLRRCFHINYIIFDIDGVLIDVKKSYNEAIKN
                     TVQFVVRNLINRDLKDLVTDKIILKFRQTGGFNNDADTSYAISLALLSHQDLQSANLE
                     KFLIEVAEHADETGIDSVEKYIKKLPQNKISPNYLININKILDYLNYPGKVGDSIVST
                     VFDEFFYGQELFLKRYKIKSKYYFGKPLIENDRIVITDNTIRKLIERVNGKIAMVSGR
                     SKIAAAYALDNKFYLLNEKNSVFLEDEDRKFAKPNPYGLKKIINKINTRNDILYCGDS
                     IEDLIMARRAEEELNQKNSNKIKTNIVFCGIYGCSSNTNELISKFMEKKADIIIKSVN
                     DLPHILNKVS"
     gene            complement(17205..18278)
                     /locus_tag="VF222_09055"
     CDS             complement(17205..18278)
                     /locus_tag="VF222_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7142613.1"
                     /translation="MDKWFESKLSEIGKLDYYKKPEKSRGLKLDANENLALKKDFIKK
                     IAIKAAEREDLREYPLHQVDDFYSQLSKYTNIDKKNLAIGNGSDQIIDLILSVFGKEK
                     RVTTFTPTFSYFIDRCLLYSIKIDKIPLLQDNSIDKEKFIQSAKKSKIIYICSPNNPT
                     GNQFDKELLIDIIKNFDDKLIIIDEAYVEFASYSFYSETIKSQNIILLRTLSKAFGLA
                     GARLGYCIANEDFSRIFKTVIQYPYAINNISLNIGSEILRNSKYIDKTINIVKKERKR
                     ILDYLKEINNEIKVFQTESNFIFFQIPDINKYEHILTQLKKDNIAVKTIGRIKDRKGG
                     HIRVTIGTKMMNNKFLRSLDKAI"
     gene            complement(18282..19607)
                     /gene="hisD"
                     /locus_tag="VF222_09060"
     CDS             complement(18282..19607)
                     /gene="hisD"
                     /locus_tag="VF222_09060"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7142614.1"
                     /translation="MINRITIKNAKLDVESIRYKISSNFSEDAIKKTREIIENVRNYR
                     DDAIKRYTSTFDKVELDSFRVTNEEIEEAYNCISDDHIKTIKLIRDRLIRNETILFEH
                     LKKISSLYIQNKNIRRIIQPISSVGCYVPGGSARYPSTLIMCITPAKLAGVKRIVAIT
                     PPKINGKIDPLTLVSADICGVNEIYKIGGAQGIAGLAFGTDSIKRVDKIVGPGGMYVT
                     IAKLLVSNIVSIDMLAGPTELVIYADNKSNAKYVALDLISQSEHSKDTLCGLVTNSYE
                     FANTVENEINNILESKISRQEIVTNSLKENGFFAISENEDLAIEFINEIAPEHLQIMA
                     KNELKIVKKITSAGLTLIGEYTPSAASDYCLGSNHVLPTMGFGKSRTSLSVLDFVRIS
                     NIVHASKKEIEEIEPYIKIITEEEGLVNHYKAVKERIRDITNQKIEDEK"
     gene            complement(19611..20591)
                     /gene="hisG"
                     /locus_tag="VF222_09065"
     CDS             complement(19611..20591)
                     /gene="hisG"
                     /locus_tag="VF222_09065"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7142615.1"
                     /translation="MAHIVKFVVPKGTIEEATLKILEEAWQKISGRARTYRIKLSDPE
                     IEVKLLRPQEIPTYVQEGLYDVGITGRDWIREANADVKILLDLEYGRVKQVIAIPESF
                     QFNTLDEMIIDHVRREKPLRISAEYLTTTANYLKSKIAYQKYFGNSNPMIITPWLRVG
                     ENKNVQIFLSFGATEAKPPEDVDAICDITETGTTLAQNNLKIIDTIMESTSVLIANKN
                     SLKDNKKREKIADMIALLKGVVEARKKLHIFVNVRKENLDELLSTLPALKRPTVSNLS
                     EEGWYGVNTIIDKNEFIKIVPIMRKLAQGLVVSEPRQILPLEEINLDKEE"
     gene            complement(20631..21968)
                     /locus_tag="VF222_09070"
     CDS             complement(20631..21968)
                     /locus_tag="VF222_09070"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEX7142616.1"
                     /translation="MDKIRKKENNKNIKKFYELDHQERLQYLKKEAHITEQDLEIFQD
                     FPKNFAFNKINNMVENAIGFIPIPLGIATNFVINGRKYLIPMAIEEPSVIAAASKAAK
                     IASIKGGFLAEADNSIMIGQIQLVSRDKRNNKDKFSYKKERDKIKKIILNNKKKLLEI
                     SNSKSRSVHAIDLQLREVSDESNNKMGYMIIIELIIDTRDAMGANVINTMCELVGSEL
                     EKLTKCTVILKILSNYSTKRMARCKAIFPKELIGGSDSVNRILYASAFAYSDIYRAVT
                     HNKGLMNGIDGVAMATGQDVRAIEAAAHAYASKDGKYRSLTKWYRSKEGDLVGEIEIP
                     LAIGIVGGISSVHPYVKVSLKILNIKKAKELALIMIAVGLAQNFAAIRALADEGIQKG
                     HMRLHARNIAITAGATDEQINIVASRLVQENNVTVNKAEEIIELINKNEINTK"
     gene            complement(21975..23087)
                     /locus_tag="VF222_09075"
     CDS             complement(21975..23087)
                     /locus_tag="VF222_09075"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142617.1"
                     /translation="MDKKLVVVMAVLAVISAIVTIFISPLDTTIPIPEPSFLSNTTNQ
                     NKGIEILAENLSTPWAIDIAKDNRIFFTERDGKIRVIDEKGILLDEPVAYIRVAQNGG
                     SGLLGIALQPNFTENHLMYIYHSYVKDNKLYNKVLLLKEKDNKIIESNTIIDSIPGSP
                     YNNGGRIKFGPDYKLYITTGDASDPLLAQNLSSLAGKILRVNPDGSIPSDNPFKDSSI
                     YSYGHRNVQGIAWHPKTKQMYSSEHGSSGFDEINLIISGKNYGWPEEECKGTNISQKA
                     IICFNPAVAPSGITFASSDKLDYQNDLIVTTLKGSHLRQIDVESGEQNNILVGYGRLR
                     DVVESSDGTLYVLTSNTDGRGIIQYNDDKILKILKP"
     gene            complement(23167..23289)
                     /locus_tag="VF222_09080"
     tRNA            complement(join(23167..23214,23235..23289))
                     /locus_tag="VF222_09080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23236..23238),aa:Ser,seq:tga)
BASE COUNT         7682 a         3661 c         3084 g         8871 t
ORIGIN      
        1 agaggattaa gagatgaagt tgatctgggg gaagttcaag aagttggaac agaatacgtc
       61 ctaactaaaa aaggaactat agacagtgat aaattctata ttcctaaaaa tctagtggaa
      121 cgctttgatg gtcggacggt ctggtttaag ttgacagaag aagaatcaca tcaatataaa
      181 gaacataaga cacatttgat ataatattgt ttattaatca ataattttat tattatttga
      241 tttttttcct tctacagcat ctaatcgttc agataccata atacatcttt tagtataaac
      301 atgtgaatac aagtaatgga ttcacatccc atcccctcca tactaattga ttgatagttt
      361 tataaaataa ttaaatatat tatatatagt gattgattat ttgattaatt gaaaacttta
      421 taatgataag ttaaggacac aaaatattat cacaattata taaattgagt acgtatcatt
      481 ttaataatat aatacttaat aaaaattagc aactggaaat cacaataaac attaactaat
      541 ataattcact aatctaattt aaattactcc gagtggagta ccaggagatg gtactggaaa
      601 ttcacgatca ataacgttaa cttcatcctc tatcagttgc catcccactc ctccactatt
      661 ctcttttact ctttcaggtc ttgtggtctt tggaatagtg aaaatagatg gatgacgtgt
      721 cagaaaattc agtgctacct gtttaggagt tcgattgtgt cttctgccaa tttctaccag
      781 taacttccct tctctactag gaggagatgg aaagttgcca tgtccaaacg gagcgtatcc
      841 cactacagct attttctttt tactacaata tggaagcaaa cgtcgttcaa ttccacgatc
      901 tcttaaattg taaagtacct gattacatgc gacccgttca gtttgtagtg cttgttctgc
      961 ctccattaaa tcttcaacat caaaattgct aacaccaatg tatttcacta ggccttcggt
     1021 tacaagtttt tccatagcac gcattgtctc actaagtgga taactgccac gccaatgaag
     1081 caaatataaa tcaagccagt ctgtctttaa tcgtttcaaa ctccgtttac aagcctttaa
     1141 ggttccttca taggaagcgt tagatggtaa taccttacta actagaaata tttcatcgcg
     1201 tcttcctgct atggcttgtc ctactaattc ctctacatgt ccatctccat acatctctgc
     1261 agtatcaata tgactcattc ctagatctaa gcccaaccta agagtccgta tggctcttgc
     1321 ctcagtttca gaatcaccgt caatcatcca tgttccttgc cctattactg gaacatcaac
     1381 accagtccaa ccaaatttat gctgaatcat tttattgtaa aatcactgcc aaaattttca
     1441 aatgacaatt tgagcaatca cgaccttctc ctacacaagg attcataata agtattattc
     1501 atagtaaaat aaagtaaaca aagtaattca ttctgcatta tacatggtaa attatgaaga
     1561 acacaaattt aatccaaact aattcttttt agaaactcta ctatcgataa tctattttaa
     1621 cttaacatgg gagttaactt cttaactcat gaaaaattcg ttaatataac ctatttctaa
     1681 tataagtaat actaaaaatt atgaaaggat ataagaataa tcctaaatct ggtaataatg
     1741 gtaagctaat gcgattatta gattatgctc taactacatt tattattcca tttgtcattc
     1801 tcctaatgaa attattagct tatgccataa tcacatttgt cattccattt atccttaata
     1861 ttataaaaaa gaaactaagt gatcaacaac gtcttttatg taataaatgt catggaacgt
     1921 tagaagtaat tgataaagaa ggtaacctat attgcaaaaa ttgtaaaatg attaaaatgg
     1981 atcgccatta acttcctaaa gttttgctga acagcaaaca ataaattgct ttcaaagtaa
     2041 ttatgagcaa atcagataca ttattttttt actacaattt taattgtata tttcctatga
     2101 atatttcact atccaaaagt aggattaaaa acagagtggt cgtttatagc gatactgtaa
     2161 tcaattgaaa atcttattat ggtgtctagg atgaattagt ttgatttttg acaatacctt
     2221 ttaattgttc ccaataattt aaatctgaat taggtataga atcgacgacc cctttcctac
     2281 atttttcact tgaccagata attcatcgta aatctggaat aagattagga gtgtggatta
     2341 tttgaaataa aaacatatta ttcctacaac catgcaactt tctgcatcta aaacataatt
     2401 acattcacca ctggaaaaga atctaattga aagagaaata cagtatttta tggacagaac
     2461 agaatgtttt gatgattatt atccttgtac caaaaagaag tattgtgatc taggacatgt
     2521 atacaattgg ataatgttgt tcatctatgc atataatatt actattacaa acaaaattcc
     2581 attcaaaata tgaggtgaat tatccttaaa ttaacagcac ctgagatccc attgaaatgc
     2641 tttaatattt aatttcctaa catcttatag aaaacatatt atgataaaat tatcttgtaa
     2701 agagtcagga ttagattgcg attacattgt agagggcaaa acagaagatg aagtcttgga
     2761 gcgtatttct gaacatgcta tcaatattca tcacatgaga gaagaagaca tatacgaaga
     2821 acatgtacct gtcgcttttt tatgccacgc aataggtaaa atgacatcaa accacaatca
     2881 gcaaaattag actacttcta tgtataaaag ataataatcc acttagtaaa aatatctgat
     2941 ctagtaagta tgtattataa ataataagta atttctctat ttaataacaa tgaaagtact
     3001 ttctgtaaat gtcggattcc caaggaaggt actcttcaat ggacaaatta taactactgc
     3061 aattttcaag gatcctgtca aaggtcctat catgttgcgg aagcttaacc ttgatggtga
     3121 caaacaagcc gatttgacag tacatggtgg acttgataag gcagtatatt cctatccagc
     3181 agaacattat gactactggc gtaaacaatt tcctaacacg gatcttgtat ggggaatgtt
     3241 tggagagaat tttaccactg aaggattatt ggaggatgct gttaatgtag gtgatcagtt
     3301 tcacatagga tctgctaagc ttgtagcaac acaacctaga atgccatgct ataaactggg
     3361 tgttagattt ggacgtatgg atgtaattag aagatttatg gcaagtggcc gtcctggtat
     3421 ttactttaag gtatcaacag aaggagaagt ccaaacaggt gacaaaataa agattatcag
     3481 aagagataaa aataatgtca cagtgaaaga tattgttagt ctttatattg ctaggaatga
     3541 tatagacaat attgaaacca tgaaaagagc aacaaaaata agagatctac cagaaggatg
     3601 gagatatgaa ttccaacaaa aaattgagca actgaaacac aaatgaaaaa ttgatattat
     3661 aacatactac ttgcttatgg attttcgatt acttcagttg gatgcatctt tgacatcgaa
     3721 ttgtatatta tgctttaata tccttattta ctgcttttat ttgaggtctg gcagaaaaga
     3781 atccagaaat cttctcagca attacgtcat aatcttcttc tagagcaaag tgaccggtat
     3841 ctagaatgta tgtctcaaca tcttttaggt ctcttttata tggatgtgct cccgattctg
     3901 ggaagatgta gtcgttttta ccccatgcaa ttaaggtagg aggttgatat tttctaaaat
     3961 tttcttgcca ttgtgaatat aatggtggat ttgttttata atcataaagc attgctagtt
     4021 gaattgcagc atttccaggt ctatccaatc ccgcctgatc gattatccaa ttgtcaggac
     4081 tgattgattc aggatttctt gttccgttaa tatattgcca ctttgtcgtc tctgctgtca
     4141 ccagatagtg aagtttcttt tcattttctg gagtattatt attccaccaa tctttaattg
     4201 gattccaaaa ttcttctaga ccttcatcat atgcatttcc attttgtata ataaaggcct
     4261 gtactttttc tggatgcatt gaaaatagcc taaatccaat tggtgcacca tagtcctgca
     4321 tgtaaagact gtatttttcc aatccaattg tatctataaa cttagaaata attttggcca
     4381 tattttcaaa agtataatca aactcatcaa ccggtggcat tgagctgtta ccatatcctg
     4441 ggtaatctgg tgctacaaga tggaattcat cagacagttt ttgtattaag ttgcgaaaca
     4501 tgtgtgatga tgttggaaag ccatgcaaca acaaaattgt tggggcattc tttggacctg
     4561 cttctctata aaaaatatcg agaccattta attttaccgt tttgtatttt gtcatgtgtt
     4621 tattttggga tttattcata agtatattat ataataaatg acatataagt agcgggaaat
     4681 cattttttga atctttcaaa tttggaagaa gattttagat ataatcatta ttaaaaagta
     4741 gtaatgttca atatggaaat agatgttttt gttatcgggg aatactatta atgaaattaa
     4801 tgagtaatca atggaataca taatactact cgtagtttaa gaattccttc acagatgatt
     4861 ttggatgtat aaattatcta tgtaaaaaaa atcatttgat aatgtaccta ctgaaattac
     4921 taataaatac gttaaaaaaa atactaacag ataaaattta gattttaata ctatcgtctg
     4981 aataattgat ttaaagttaa cgtactgatt atttcaatat aaatgtcttt atttctatag
     5041 tcacaaatta atatagaata ttatcgtatc aatttactca ttactcggat tataaccaaa
     5101 tatattaaga taatttttcc tttgtttaca gactaggttt gtactattaa gttaaagatt
     5161 tgttagaact cagagaggta attaatacaa atatctttgg gttagtaaag ataaagataa
     5221 taagttaaaa aacaatcaaa aagatatgga tgaaaattta atcgaaagaa aaaaaagatt
     5281 agagaaagaa ttagatagta aagtatctaa tgattatcga gaattcataa acaaatcaaa
     5341 taattttttg caacaatcaa gagaaaatac taaaaaatat tatcaagatt tactttctaa
     5401 agaattagat tcaatggaaa acgctataaa atcgggtagt acattacaag caatgagtca
     5461 taggctaaca gcaaatgttt atgaatcaat tcttcagaat acttaatatg cgatttttta
     5521 tatgattata aagattaagt cacagtagat ttgtaggtta aattatggac caatatagaa
     5581 tactttttgg tccacttcac gaacaatatg atctatattt gctattacta tatcatgctg
     5641 atttgttcaa tgaagattta gaaactaaca ttaattcaaa ttaagcgctc gaattttttt
     5701 gcttagagca aaaagaaaca ataagacatt atcctaaaat actaaaaata ttctttgatt
     5761 ataatctaga ttcaaatatc tttataggaa caagcagatt ttatttataa taacagttaa
     5821 ctactatgaa tactctaata agtagtatga ctcatcatat cgcaaaaata actgagatag
     5881 ttggtagttc agaaaaaggt tggcaagagg cagcccaagt tgctctagat gaggcaaaaa
     5941 aaacaatacg tgaaattaca ggtcttgaag tggtagacaa gactgctacg gtagacccaa
     6001 atacaggaag tattactaaa tataaaacta caataaaaat agcatttggt gtagaacgtt
     6061 aagaactaac taataatttt ttcattctat ttgcaatagt tccaaaataa tagaagatac
     6121 tattcctgac tctcatttca aagataatta tttttgatat atttgataga ggctattcat
     6181 gttttatcaa aagaagagat gacaggatag tataatcaaa ttaaaagaat attgaaaaaa
     6241 actccttttg tttctaaaat gtttcagcat tgatgagcaa gacagattgc actggtttta
     6301 gaattaatct tggattttaa atcatcgatg tcgggttttc catcaggatg gattttaaat
     6361 tctacaggtt ttcctccata aaactttgca taggaagcat aaggtgggta atgtggtcca
     6421 ggcattagta tttcatcacc aggactgaca acggatgcaa gagtcatatc tagtgcttct
     6481 gaaactccat ttgtaactag aacgtcattt tcagttacat ccaaagcctt ttcctttctc
     6541 tttttctaca atggcttgtc gaagctctgg taagccttcg gatgcagtat aaaagttttc
     6601 atcactttga actgctttaa ttaaggcatt tttaatgtga tcgggagtag gaaagtcata
     6661 ctgtactgga tcaccgatat ttagatatat tacatttttg ccattcttct ttttcatatg
     6721 ctcttgcctg cgtaatgata tctctaatag catattcaac actggctgcc ctcgctgaaa
     6781 ctttcaattg agtatccaat ctattataaa ttacctagat aaagtattct ttatgcccgt
     6841 aacataactt taaccattga aggttatctt ttgtaatttg tgcaatcatt ttaaaaattt
     6901 ttaaactttc taaaatcaaa ataggattag agcagggtat cgcgacatga ctagaatttg
     6961 ttgtttatac ttagcaatag ctttttttag attgcatatt atgagacata caaagaaaag
     7021 aattctaaat attagataaa taatgaactc ctggatagac tatattctta ataaaattac
     7081 aaaatattgt aaagatttat ataaccaatg atttgagccg ttgttttttt gtttatatct
     7141 aagattttta aaaaaaattc aataccgttt agatataatc gactttgctt atcattttat
     7201 caattataaa aaattaccca attaattaaa attcgttgta gcacgagtcc tccgtcagtc
     7261 ctctgcattt tttctggttc ttatttgcat ctattaatac cagaagttta ctggtctaat
     7321 gatattatta atataatttg attaaagtta ctttcatagt aattttaatt ctaatgttaa
     7381 caaacaagca gattttgcat cttaaaaatg caaattttat catcatcaat aaaaataaag
     7441 aattttgtag gttttattta gactacactg tcaagtctgg aaaaagtaaa aaagattcgc
     7501 atttttattt gctcaagtaa aagtaattta gttataaatt ttggttctgt tgaatctctg
     7561 gatgttccag ttggctattg atttttgggt gttgataatt tacggggata ccatctttca
     7621 ccatcatgaa gccaaaggat gttattttca atttcataat ctataacttt tttttacaaa
     7681 tatgcctatc tatttcttca tcaatcaatc aatgaataac cacaattttt acacttagtc
     7741 cgattcatct ttatcggctt tcttatttac aaattgtttt tcatgactgt ttatgataat
     7801 ttgaatatct tctttaagtt cactggctaa taccatatta tcattatata gtttttcatt
     7861 ttcagttttt cctctaaagc gatttctctt ttttaataca tgattcattt gaccagatga
     7921 ttatatctct tgaaagaagt ttagaagaaa acatcgatga aggtattaaa actttagaat
     7981 atcacattag gcctcaaaat tatccaatag taaacgagat gttccaatta taaattgaaa
     8041 cttttgaaat gggtaatcag aagtaaatga agggtctaga atcgatgaaa catatcgtaa
     8101 attcaagaat aaatagatta gaatatgaac tgaaaatagc aatggagata gagtatagta
     8161 gtaaaattgt ctatcaacta gaaatgttgg aatgttgcaa agtcataata aatgtggatt
     8221 tgctaagaag aacaagaaaa gaagaaaatg gcatatctcc atttttctag tattaaaaca
     8281 atatcaaaca accgaataat attctaatct caataaacaa ttcttttaaa gaacaattta
     8341 gtgatatgtc tagatagatt aacctactac atgctgaaat tcgaaatgtt cttttctttc
     8401 ttctcttgta ataaagttga gtcggcatac agggcattgt attatagaat ttgatgacat
     8461 aatataataa cacatttgct actaatatag tttgactaat tcaaatatca acttctttga
     8521 taaagtctta cttgtttctg acatagtggt caaattttcg ctttacttct tcactattgc
     8581 cttaaggaac aatgtttaaa tttccattaa tgtccttatt cctttgagaa gcataagcct
     8641 tttcattata tggtgtcctc cgataagatc agatagaaac tcgtactgtt tttgtatacc
     8701 ttctatggaa cttgtaacat caccaatgag ttatccatct ttggttttga ataatttgaa
     8761 taggattttt ataaaaagtt ggctttgtcg tttctgtatt gggggagggt gttattactg
     8821 ttgttgtatt cttactactt tgtaggtagg aaaagtagta ttcttttttt gaatacttcc
     8881 ccttatgcat tattactata ttccagtata attgaaaatt acaatgatac ctatatagtt
     8941 tgagttaata gttgaactca tttcttatct agtcacttcc attacttgaa ttagatggtc
     9001 tttaaacaat ggattattat acattttttc gttaaaacaa tcgtcacata tccagaattt
     9061 tgactgtttt tcaattatgt tttttactat ggctatttta cttgctgttc ttttgcatct
     9121 tatattattg tcggtggaat tttgacatct catttcttat tctttatctc actatcattt
     9181 actattttat caattcttcg tcagtcagcg catttaatct ccaagacaaa tacacttcaa
     9241 aaatcatatg tttgatctgt ttatctgtgt attctttatt aatatattgt cgtaactttt
     9301 ctccttttgt catttcttat tcttcttctt ccgcatgacc tattaagttt ataagaattg
     9361 atgagtataa taatcattat tttagacatt tatcatgatt tttaactttc gaaaacttca
     9421 cagcaaatct aaaattttct gggtgttctt ttatttcagt tgttaaccat aaacttagaa
     9481 ggaattctat aaaaagtgaa acctatttca acaaatcaaa aatttgaatt agtatgaaaa
     9541 ctaatatcta ttatcattct cctgaataga tagaatgagt cttttccatt taccataact
     9601 ctatccagaa catcctattc caatactatt ttttaccctg agaaggctaa aacttttcta
     9661 caacctacta tttaacagtt attaatatat caaaggatac tttgaaatag taggtctatt
     9721 aatatacaat tataaaaaat tgtataaatt tcaattcttt ctaactgaaa gaaagagaga
     9781 ggaacaataa attaatgcct atattaccca aaaccaaaag cgtctacgac tctagtatat
     9841 ttgaaattaa aaacaacaac ttagaagaag gtatagaatg ttttagtcat tggctttcaa
     9901 ttgaagaagg aaatagtgaa gatagtaaca agaatgttca attaaccctt atagtatttg
     9961 aaaagaagaa taacaaattg ttacttcatg ataatcttca caattcggat attgaagttg
    10021 ttttcactga tggcgtccca ttctgtaatt tatgccgtac tgatgattgt gtacatgcag
    10081 gttttgctat atgtgcaaaa caaaatcatt gttgataata tactgcaagt attaacaatt
    10141 aaaaatttag aatgattaat gagattttaa ttcttcataa tcatattttt atcatatgat
    10201 catgattctc taacctatgc gatttaataa attttagtac tcaaagcaaa tgattttaaa
    10261 tttgatattt atgatttaaa actaagagaa ataattcctc catataatct gtataataaa
    10321 tagtagttta ttgtgcaacc aactaactat actaaatgat tacaaaaaat cagtaattag
    10381 ataatttcta ctatttttaa ttaatctttt aatttgtttt gtgttagttt tttctgaagg
    10441 taacctagaa aaattacaaa attgatgcaa aaaatctatt tcttcattta acaaaaaatc
    10501 tatatccaaa ttttcttctg ctctgaaaag atagttaaat acaagtttct gattaatttg
    10561 ttctaaagtt ataaggctat gatatattct aaccatgtct gaaattttat tagttatatt
    10621 attatttatt agcattttca taaaacttct ctttgggata ttaaatatgc cattcattat
    10681 acatctatta aatatagata atatttcaaa atagtcatct tttccactat atcctattat
    10741 tgttgttgga agtagtttta atatttgcaa attcctattt aaaatatatt ttttgtctat
    10801 tactaaatat gatctaccaa atgtaagaat ggcatttaat atttgattag gaagacgctc
    10861 atcgagtacg tcattatcag attttatctc gactggtatg aatgttatat tttcaatttt
    10921 gcatttctct tgtttagaga attctgtaaa ttgctgtgat ctcataagaa tgttatgctg
    10981 aatatcatat tctaaatcaa aatattcgtt tttcatttgt ttagctgaat aattagtttt
    11041 attttgttta ctttctttat taatcaaagc aattactaaa tcaaaattac gaaaatatcc
    11101 ttcctcttta atcttaattt catgagtttg agaatttaaa aatgggagaa gatggttatt
    11161 attgatatga ccatttttaa ctgaactctt aaataatttt tttagttcca attgattcat
    11221 agatggtaaa ctaaaactat tagctaaata ataatagcct atcgatacaa gtttttgttc
    11281 tattaattct ctattaccat gtattccata gattccgatt gattaatgta atatatttct
    11341 gtgtaataca atataacgta atataactaa attatggata ttaaggacag cttaaattat
    11401 tatttgtata tttttactat aatgtgccat ttttggatat tttagagcta caatttcaga
    11461 atgtcttgta agatattatg attaagagga attctataaa tatttttatt gataacttaa
    11521 gctgtattat tgtattatta ctttctaaat atcaatacta taagagatat aaacattgat
    11581 aattataata ggatagaatg gtatcgttag aaacaggaat tgcattaatt ataattggaa
    11641 taattttgtt agtattgaat aggttcattc cctcgcttcc cgcaagacta ctgtatatta
    11701 taggctcgat tgttctagtc attggaataa ttatggttat attatatttt gtatttggaa
    11761 tcatttaatg aattcgaaat tataaattta aaattcatct cttttatgtt ttataaaaaa
    11821 atttagtgaa tatgaataag gaattttata aataatatta ttatataatt gcagagcatt
    11881 aatgattgat ccaagtaaat caaaattaga tatttatgta atatccatgt gtttaataag
    11941 tacagataag attataaaat aatacataaa tttattacga aaccaaatta atcctttaat
    12001 aatataacca ttttatatta gtttttacgc ctatgaattt attttgtcca cattcagtta
    12061 gacgattctt ctttcttaga tttttcccaa caaattctat atctcgttat tgttcaagcg
    12121 aacttctttc ttttcttttt caccataaat attatatgaa gtgggtcatc aatttcttta
    12181 cataaaaata ttcactttat cattcatgaa agttatataa tagtttttct ttcatctgtg
    12241 aatcactgct attaatttaa taaaatatag aaaaaactaa attaactttt gtgtgaatgg
    12301 ttttaaaaat taagacattt atatctatca tcatttctac aaatatttac tatgataata
    12361 tttaattaat ttagatatgt acaatgccaa ctccttatcg ctataacaaa aaaaatatta
    12421 ctaatttttc agaacttaat actttaattg attattaatc tacatcatat aatttaagtt
    12481 acttaatata tatctaacac atgataagct aatatcgata tatagaaaaa attgcaatat
    12541 tagctatata cttcaagtaa tattttctat agaaaaatta tttatatctg aataacaaat
    12601 gtattattat cgatattaat gtactataag tgctcttcag taggaactat tggtaaattt
    12661 ataataaata ataaagatac tttaacaata gttttatcgt agttagtaag tgtagatata
    12721 gtaatattta gaaaaacaat tgaattgaat ttcttcttaa cagttatcaa tgataataat
    12781 aatcattact tctgctaatt tacttgtgac ttttattgca gaattaacct aagtataaaa
    12841 actaatgtaa ttgcagtatg attgttaact tcttttagat tttctcaaaa tctctttatc
    12901 attagacaat acatttttaa attacattat tttatatata atcaaggaag gattagattt
    12961 aggcaaaaga aaagttttga atgaggctgc actaaaagag cttgttatgc ctcatgaagg
    13021 agaaatgttt ggaagagtta taaagctagt aggaggcgat aatgttattg tcaaatgtaa
    13081 tgatggtaaa gtacgtacct gtcgtattcg aggaaagata aaaagacgaa tgtggataag
    13141 agaaaatgat ctagtacttc ttgcaccttg ggattttcaa tctgaaaagg cagatataat
    13201 ttggagatat attgcagctc attctgataa gttagaacaa gaaggacatt taaaaggttt
    13261 aaataaataa tacattcgac tttatttaag ttttaattaa tattccttta ctattagaat
    13321 tttatattat aaatagataa ttgatacatg caacaaacgt tattttaata atatcatatt
    13381 cttacatgaa tattatttct ttttaaaaat tgttttactc ttttaacagt tgatttatca
    13441 taatgaaata tagatgctgc taaagctgca tctacctttg ttttttggaa aacttccagc
    13501 atatgaattg gttttccaca tcctccagag gctatgactg gtattcttaa ttctttgcaa
    13561 ataattctgt tcaattctaa atcatatcct tcttcagttc catctgcatc aatacttgtt
    13621 aaaagaattt ctcctgcacc aagtttttct acctttttag cccattctat tgcatctatg
    13681 cctgttcttt cttttcctcc atatatcatg acttcaaacc aatagtgctt attttgtttc
    13741 tttttatgct cataattatc ttttagagtt ttcttagggt caacattaga ataatttttt
    13801 ttaacatcta ttgctactac tatacattgt tttccaaaaa tagacattaa ttctgtaatt
    13861 aattctggat tttttatagc agatgtattt attgaaactt tatcagctcc acttagtaaa
    13921 atatctctag catcttctaa ttttttgata cctccaccta cagtaaaagg tatgtcaata
    13981 actctagcaa catttcgtac tacattttta ataatatctc gtttctgttc cgaggcagtt
    14041 atatctagaa aaacaagttc atctgctcct tgttgactgt atctttttgc aaattcaact
    14101 gggtctcctg catctttcac ttctttaaaa ttaatacctt taactaccct accattgtct
    14161 acatcaagac aaggaataat tctttttgct aaggtcattt ttaaactact attagtgctt
    14221 tttttacgtc tatctttcca tcatagagag atttgcccag tataactgca gaacaaccaa
    14281 tatttttgac tcttattata tcttcaatac tggatattcc accactggca attatttttg
    14341 ctccatttat gtttgaagca taggaaagtg tatctaaatc tgggccattc aaagttccat
    14401 ctctatccac tgatgtgagt aaaaattctt taattccctt ggccataaat aattttatag
    14461 cttcaactac cttaaatccg gatggttgcc tccatccatc aatcatgaca atacctttat
    14521 tttgatctat tgaaattact attttctcta ttttatcctt taattgttgt aacacttgag
    14581 gctgtttgta tgccaatgtt cctataacaa ttttttctgc ttttttttca aacatcctgt
    14641 taatagcatc aatagatctt attcctccag ctacctgcac aggaattttt attgaatcta
    14701 ttatttttaa aataatttct gtattatttt gattattatt ccatcctgta gtcaaagtag
    14761 catcaagatc aactacatga attatatttg caccttgact ttccattttt tctgcaattt
    14821 ctataggatt gtcactataa acaattttat ttcgaagatc tcctttaact aatctcacta
    14881 cctttccttc tagtatatca attgcaggta ttatattcaa tatttaccac cacatattgc
    14941 caaaaaattt tttattattt tagctccatt tatccctgat ttttcaggat gaaattgtgt
    15001 tccaaaaaga ttatttttct ccacaactac tggtatatta cctccgtatc gagaattcgc
    15061 aactattact tttttatctc ttgaaataac acggtatgaa tgaacaaaat atacccaaca
    15121 tccatctttt aatccttgta ggattttaca tgctttttct gttatttcta aattattcca
    15181 tcccatatgt gggattttta ccttttttct tggaagcata attactcttc cccttaaaat
    15241 tttcaaacct tctaactttc cctcttcgct attattaaat aacatttcca ttcctagaca
    15301 aatgccaagt ataggaaaat ttttttcaaa cagattatca aaatcgtctc tatatggttc
    15361 tattgatcga atagcaggat caaagttccc tactccagga aggattaatc catcatgttt
    15421 atgaatgctt tttaaagatt taattacgga aacttttgtt gctccatttc tttccaaaga
    15481 gcattttaaa ctataaatat tacctgcacc ataatcaaaa attgcaatat cattcaatat
    15541 tacattactc cttttgtact tggaataccg tttctttgat aatctataca tgaagccatt
    15601 ctaaatgcta ttgcaaatga ctttattgct gcctctattt tatggtggtc attttcgcca
    15661 tattgtatat taatatgagt acatgcattc agattttgta ataaactttc tataaaatgt
    15721 tccaaatcct ctcttgcaat tccttcaatt tcctttcttg tcaatcctaa atttatttta
    15781 taggtttgtc ttttgattag atcaattgct gaaattgcca ttacttcatc cattggaatt
    15841 attgcatatc catatcttct aatttgcgtt ctatttgata acgcatcatt tagagtctga
    15901 gagagagtta tagccacgtc ttcaattaaa tgatgagaaa tactatcatt tgattttgct
    15961 tccaaataga tatctaccat actatgcttt ccaaacgctg agattaaatg atctataaat
    16021 ggaattccag ttttaatttc cacattacca gagccgtcaa tattaacact cacacttaca
    16081 ttggtctctt tcgtaaatct atctttgact gatattctat tctgagattt atccaagtca
    16141 agaggtttcg aattcaacta acaatattcc ttatgatact ttatttaata tatgtggtaa
    16201 atcgtttaca cttttaatta ttatatctgc ttttttctcc ataaacttac taattaactc
    16261 atttgtatta ctactacaac catagattcc acaaaaaact atattagttt tgattttatt
    16321 agagtttttt tgatttaatt cttcttctgc tcttctagcc attattaaat cttcaataga
    16381 gtctccacaa tagagaatat catttcttgt attaatttta tttattattt tttttaatcc
    16441 atatggattt ggtttagcga attttctgtc ctcatcctcc agaaatacac tattcttctc
    16501 gtttaacaaa taaaatttat tatctagggc ataggctgct gcaatcttac ttcttcctga
    16561 aaccattgca attttaccgt taactctttc tattagcttt ctaattgtat tatctgtaat
    16621 cactattcta tcattttcga tgaggggttt tccaaaataa tattttgatt taattttata
    16681 tctttttaaa aataattctt gtccataaaa aaattcgtca aataccgtag aaactatact
    16741 gtctccaacc tttccaggat agttcaaata atcaagaatt ttattaatat ttattaaata
    16801 atttggagag attttattct gtggtaattt tttaatgtat ttctcaaccg agtctatccc
    16861 cgtttcatca gcatgttctg ctacctcaat tagaaatttt tcgagattag cactttgaag
    16921 atcttgatgt gataatagtg ctaaactaat tgcatagctt gtatcagcat cattgttgaa
    16981 acctccagtt tgcctaaatt tcaaaatgat cttatctgta actaaatctt ttaaatctct
    17041 attaatcaga tttcttacta caaattggac tgtatttttt attgcctcgt tataagattt
    17101 ttttacgtca attaaaactc cgtcaatatc aaaaataata tagttaatat ggaaacatct
    17161 tctcaatgta tgaataaaat taatattatt tattttttga tacattatat tgccttatct
    17221 aaggatctta aaaatttatt attcatcatt tttgttccga tagttaccct aatatgacct
    17281 ccttttctat cttttatcct tcctattgtt ttaactgcga tattatcttt ttttaattga
    17341 gttaaaatat gttcatattt atttatgtca ggaatttgaa aaaaaatgaa attagactca
    17401 gtttgaaaaa cttttatttc attattaatt tcttttaaat aatctaatat tcttttacgt
    17461 tctttcttaa caatattaat agttttatca atatattttg aattccttag tatttcactt
    17521 cctatattca aagaaatatt atttattgca taagggtatt gtataacggt tttaaatata
    17581 cgtgaaaaat cttcgttagc aatacaataa cccaatcttg ctcctgctaa tccaaatgct
    17641 tttgataacg tccttaatag tattatattc tgacttttta ttgtttcaga ataaaatgag
    17701 taactagcaa actctacata cgcttcatct ataattatta atttatcatc aaaattttta
    17761 attatatcaa ttaatagttc cttatcaaat tgattacccg taggattatt tggtgaacaa
    17821 atgtatatta tttttgattt ttttgcgctt tgaataaact tttctttatc aattgaatta
    17881 tcttgtaaaa gaggtatttt gtctattttt atggaatata ataaacatct atcaataaaa
    17941 taagaaaaag taggagtaaa ggtagttact cttttttctt ttccaaagac tgacaggatt
    18001 aaatctatta tttgatcaga gccatttcca atagctaaat tttttttatc tatgttagta
    18061 tattttgaaa gctgtgaata aaaatcatca acttgatgta acggatattc tcttaaatct
    18121 tctctttctg cagctttaat agctattttt ttgataaagt ctttttttaa agcaagattt
    18181 tcgtttgcat ctagttttaa tcctctactt ttttccggtt ttttgtaata atctagtttt
    18241 cctatttcac ttaattttga ttcaaaccat ttatccatat tttatttttc atcttctatt
    18301 ttttgatttg taatgtctct tattctttct ttaacggctt tataatgatt aaccaatcct
    18361 tcttcttcag ttattatctt gatataaggt tcaatttcct caatctcttt ttttgaagca
    18421 tgaactatat ttgaaattcg cacaaaatct aatactgata aagatgttct tgatttacca
    18481 aatcccatag taggaagaac atgatttgag cctaaacaat agtcactagc tgcagatgga
    18541 gtatattctc caattaaagt aagtccagca gatgtaattt ttttaactat ttttaattca
    18601 ttcttagcca taatctgtaa atgttcggga gctatttcat taataaattc tattgctaaa
    18661 tcttcatttt ctgatatagc gaaaaaccca ttttctttta aactatttgt aactatttcc
    18721 tgacgtgaga tcttactttc taaaatatta ttaatttcat tttcaactgt atttgcaaat
    18781 tcatatgaat tagttactaa tccacacaac gtatctttgc tgtgttctga ttgggaaatc
    18841 aagtctaaag caacatattt tgcattagat ttattatctg cataaattac taattccgta
    18901 ggacctgcta acatatcgat tgatactata tttgaaacca ataattttgc aattgttaca
    18961 tacattccac ctggacctac aatcttgtcc actctcttga tgctatcagt tccaaacgct
    19021 aatcctgcaa taccttgcgc tcctccaatt ttgtatattt cattaactcc acaaatatca
    19081 gcagaaacta acgtaagggg atctattttt ccatttatct tcggtggtgt tattgctact
    19141 attcttttaa ctcccgctaa ttttgctggt gttatacaca ttattagagt acttggatat
    19201 ctggcagatc caccaggaac gtaacaacca acgctagaaa taggttggat tatgcgtcgt
    19261 atgtttttat tttgtatata taaggatgat atttttttaa ggtgttcgaa aagaatagtt
    19321 tcatttctta tcaatctatc tctaataagt ttaatagttt taatatgatc atcagaaata
    19381 caattgtatg cttcttctat ttcttcatta gtgactcgaa atgaatctag ttctacttta
    19441 tcaaatgtag aagtatatct ttttatagca tcatcacgat aatttcgaac gttttctata
    19501 atctctcgtg tttttttaat tgcatcttca gaaaaattac tcgatatttt ataacgtata
    19561 ctctctacat ctaatttagc atttttaatt gttattctat taatcaatta ttattcttct
    19621 ttatctaggt tgatctcttc taatggtaat atttgtcttg gttctgatac aactaaaccc
    19681 tgtgctaatt ttctcataat tggaacaatt tttataaatt cgttcttatc aattattgta
    19741 ttcactccat accatccttc ttcactcaaa ttgctaaccg taggtctctt taatgctggt
    19801 aaagtggaaa gtaattcatc taaattttct tttctaacat taacaaaaat atgcagtttt
    19861 ttacgtgctt ctactactcc ttttaataac gcaatcatat ctgctatctt ctctctcttt
    19921 ttattatctt taagagaatt tttatttgca attagaacag aagtcgattc cataattgta
    19981 tctataattt ttaaattgtt ctgagctaaa gttgttcccg tttcagtaat atcacatatg
    20041 gcatccacat cttctggtgg cttagcttct gtagccccaa aagatagaaa tatttgaaca
    20101 tttttatttt cgccaactct aagccaaggg gtgataatca taggattact atttccaaaa
    20161 tatttctggt atgcgatttt agattttaaa taatttgctg tagtcgtaag atattctgca
    20221 gatattctta gtggtttttc tcttcttaca tgatctataa tcatttcatc caaagtatta
    20281 aattgaaaac tttctggtat agctattact tgtttgactc taccatattc taaatcaagc
    20341 aatattttta catctgcatt tgcttctcta atccaatctc tgcctgtaat tccaacatca
    20401 tataatcctt cttgtacata agtaggaatt tcctgtggcc gcaaaagttt aacttctatt
    20461 tctggatcac ttaacttaat tctataagtt cgtgctcgac cacttatttt ttgccatgct
    20521 tcttcaagaa tctttaaagt tgcttcttca atggttccct taggtacaac aaattttact
    20581 atgtgagcca taactataac aaaaaatttt tcttcttatc agatatatat ctacttagta
    20641 ttaatttcat ttttattaat taattcgata atttcttcag ccttatttac agtaacatta
    20701 ttttcttgta caagtctgct tgcaacgata ttgatttgct catcggttgc tcctgcagtt
    20761 atggctatat ttcgagcatg tagtctcata tgtcctttct gaattccttc atccgctaaa
    20821 gcacgaattg cagcaaaatt ttgagctaga cctactgcta tcattattaa agcaagctct
    20881 ttggctttct ttatatttaa tatcttcaag gatactttca catacggatg tactgagctt
    20941 attcctccta ctattcctat agccaatgga atctcgatct ccccaactaa atctccttct
    21001 tttgatctat accatttagt caacgatcta tattttccgt ccttagaagc atatgcatgt
    21061 gcagccgctt ctatagctct aacatcctgc cctgttgcca tagctactcc atcaattcca
    21121 ttcatcaaac ctttgttatg tgtaaccgct ctatatatgt cagaatatgc aaatgcagat
    21181 gcgtataata ttctattcac actatccgag ccacctatca actcttttgg aaaaatagct
    21241 ttacatctag ccattctttt tgttgaataa ttcgaaagaa ttttcaaaat aactgtacat
    21301 ttagttagtt tttctaattc cgaaccaact aattcacaca ttgtattaat tacattagct
    21361 cccattgcat ctcgtgtatc tataattaat tcgattatta tcatgtagcc cattttgttg
    21421 ttgctttcat cagagacttc ccttaattgt aaatcaatag catgtacgga tctacttttt
    21481 gaatttgata tttccagtaa ctttttttta ttattcaata ttattttttt tattttatct
    21541 ctttctttct tgtaagaaaa tttgtcttta ttatttcttt tatctcgtga aactaattgg
    21601 atctgcccaa tcatgatgga attatctgct tcagctaaaa atcctccttt tatacttgca
    21661 attttcgcag ctttacttgc agcagcgata acagatggtt cttctatagc cattggaatt
    21721 aaatattttc ttccatttat aacaaaatta gtagcaattc ctaaaggaat gggtataaat
    21781 ccgattgcat tttcaaccat gttgttaatt ttattaaaag caaaattttt tggaaagtcc
    21841 tgaaagattt ctaaatcttg ctcagttatg tgagcctctt ttttcaaata ttgcaagcgt
    21901 tcctgatgat ctaactcata aaattttttg atgtttttgt tgttttcctt cttacgaatt
    21961 ttgtccacaa gtgtttatgg tttaagtatt tttaaaattt tatcatcatt atattggata
    22021 attcctcttc catcggtatt gctagtcagt acatataacg taccatcact agattcaaca
    22081 acatctctaa gtcgaccata acctactaat atattatttt gttctcctga ttctacgtct
    22141 atttgcctta aatgagagcc tttgagggtt gttacgatta gatcattttg ataatctagt
    22201 ttatctgatg aagcaaacgt tataccagaa ggtgccactg caggattaaa acaaataata
    22261 gctttctgag aaatatttgt tcctttacat tcttcctctg gccatccata gttctttcct
    22321 gaaataatca aattaatttc atcaaatcct gatgaaccat gttctgatga atacatttgt
    22381 tttgttttgg ggtgccacgc aattccctga acgtttctat gtccatatga ataaatagaa
    22441 gagtctttaa atggattatc tgaaggaata gatccatctg gatttacgcg taatatttta
    22501 cctgcaagtg aagaaagatt ttgtgctaaa aggggatcag acgcatcacc agtagtaatg
    22561 tatagcttat aatcaggccc aaactttatt cttccaccat tattatatgg ggacccagga
    22621 atagaatcaa ttattgtatt cgattcaata attttgttat ctttttcttt taaaagtaaa
    22681 actttattat aaagtttatt atcttttacg taactatgat aaatgtacat taaatgattc
    22741 tctgtaaagt taggttgaag agcaattcct aataatcctg aacctccatt ttgtgcaact
    22801 ctgatataag ctactggttc atcaagaaga attccttttt catcaattac tctgatcttt
    22861 ccatcgcgtt cagtaaaaaa tattctatta tcttttgcaa tatctatagc ccaaggtgtt
    22921 gatagatttt ctgcaagtat ttcaatacct ttattctgat tagttgtatt actcaagaat
    22981 gagggctctg ggataggaat agtagtatct aacggagata tgaaaattgt aactattgca
    23041 cttataacgg ccaatacagc cattactact actaattttt tatccattaa tgcgagttta
    23101 attattaaag tctaataaat tttatatcta gtcaaattgc ttagataaac tttttagcaa
    23161 tataagcgca gatgaggaga tttgaactcc tgatcgcccg aaggcgaatc ggcttgcttt
    23221 aaccttgaag attactcaag gccgacgcat tattgtgttc tgtatagaag accactctgc
    23281 cacatctgca caaagata
//
LOCUS       DASVEV010000199         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1641379, whole genome shotgun sequence.
ACCESSION   DASVEV010000199 DASVEV010000000
VERSION     DASVEV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641379"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..690
                     /locus_tag="VF222_09085"
     CDS             52..690
                     /locus_tag="VF222_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7142618.1"
                     /translation="MNIINMINNDNSEEKDEEQTNDNSNSLKEELKEEQTNDNSNSLK
                     EELKKTKIELEETKKKAETNLNKLKYMMADFDNYRKQIEKQMTSTLESDRSNLLSRFL
                     TVHDDYQRAFDIIKKNQDLDEVFLNGLEGILKNLDNILQSEGVTPIETIGKILDPSKH
                     DVISFSYNDDLPENTITNEIRIGYMLNDKVLRPSLVEISKKNIMNNNIDESN"
     gene            711..2612
                     /gene="dnaK"
                     /locus_tag="VF222_09090"
     CDS             711..2612
                     /gene="dnaK"
                     /locus_tag="VF222_09090"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7142619.1"
                     /translation="MGKIIGIDLGTSNSAAAAMIGGKPTIIQAAEGASVGGKAFPSVV
                     AFTNTGELLVGEPARRQMISNPEGTVLAAKRKMGTDFKFNVRGKIYTPQQISSFILQK
                     IKKDAEAFLGDTVDRAVITVPAYFNDNQRQATKDAGDIAGLQVVRIINEPTAASLAYG
                     LDKTQKDLKIMVFDLGGGTLDVTIMEMGGGVFQVKSTSGDTQLGGTDMDNTIIEYILQ
                     EFKQQTGLDVRNDRAAMMRIREAAEKSKIELSNLVSTEINLPFLAYDPSTGPKNLIVN
                     LTRSKLEELLRPIVEKCKNPMQQAIQDAKLTPAEIDKIIMVGGPTRMPIVRQFVATIM
                     GKEPERGVDPMEAVALGAAIQGAIIAGDVSSDILLVDVTPLTLGLEVLGGLKETLIER
                     NTTIPTKKSKVFTTAADYQSAVTIHVVQGERPMAADCVSLGTFNLTGIPPAPRGVPQI
                     EVTFDIDANGILNVTAKDLGTSKEAKITITATTKLSKDEIEKLKKESEQYSEADMKKK
                     EEAEIRNEAENLVYSAEKLVSQDFKDKIKPEQTEKINKAVQELKDVIASNNIENLKDK
                     IKEIKNLLAEISTEIYKTVGVANQAGGGQGTNTEDFTNQSETNRGDTSNPADTSDRSS
                     TDKSTSPGS"
     gene            2677..>3066
                     /locus_tag="VF222_09095"
     CDS             2677..>3066
                     /locus_tag="VF222_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7142620.1"
                     /translation="MTNKRDYYEILEIQKNASKDEIKNTYRKLALKYHPDRNKSPGAE
                     EKFKEISEAYAVLSDDEKRKKYDTYGHVGSEDVFRGSEANFEEIFRDMGFGGFAKTIF
                     EQMFGGRGGRAGGFEGSTDPFSGFSFNI"
BASE COUNT         1216 a          435 c          572 g          843 t
ORIGIN      
        1 ttcggtttca aaattatttt caacgatatc taattatccg gcattgtttc tttgaatata
       61 attaatatga tcaataatga taattctgag gaaaaagatg aagaacaaac caacgataat
      121 agtaattcct taaaagaaga attaaaagaa gaacaaacca acgataatag taattcctta
      181 aaagaagaat taaagaaaac caaaattgaa ttagaagaaa caaaaaagaa agcagaaacg
      241 aatctgaata aattaaagta catgatggct gattttgaca attatagaaa acagatagaa
      301 aaacaaatga cttctacatt agagtcagat agatctaatc ttctttctcg atttttaact
      361 gtccatgatg attatcaaag agcatttgat ataataaaaa agaatcaaga tcttgatgag
      421 gtttttctta atgggttaga aggtatttta aaaaatttag ataatatttt acaatctgaa
      481 ggtgtaactc caattgaaac aataggaaag attttagatc caagcaaaca tgatgttatt
      541 agcttctcat acaatgatga tcttcccgaa aatactatta caaatgaaat acgtatcggt
      601 tatatgctta atgacaaagt tctaagaccc agtcttgttg aaatatccaa aaaaaatata
      661 atgaacaata atatagatga atcaaactaa aatataggtg attattaaac atgggaaaaa
      721 ttattggtat cgatctagga actagtaact ctgcagcagc agcaatgata ggtggtaaac
      781 ctacaataat acaggcagcg gaaggtgctt cagttggtgg caaagctttt ccatctgtag
      841 tagcctttac aaatacgggt gaactattag tcggagaacc ggctagaaga caaatgatat
      901 ccaatcctga aggaactgtt cttgctgcta aacgtaagat gggtacagat tttaaattta
      961 atgtaagagg aaagatttat actcctcagc aaatttcttc gtttatttta caaaagataa
     1021 aaaaagatgc tgaagcattt ttgggagata cagtagatag agccgttatt actgtacccg
     1081 catatttcaa tgataaccag aggcaagcaa caaaggatgc aggtgatata gcaggattac
     1141 aggtagtaag aattatcaat gagcctacag ccgcttcact tgcttacgga ttagataaaa
     1201 ctcaaaaaga tttgaaaatt atggtttttg atctgggcgg aggaacatta gatgtaacta
     1261 ttatggaaat gggtggagga gtgtttcaag ttaaaagtac ttcaggagat actcagcttg
     1321 gaggaaccga tatggataat actattattg aatatatttt acaagaattt aaacaacaaa
     1381 caggattaga tgtacgaaat gatagagctg ctatgatgag aataagagaa gcagcagaaa
     1441 aatcaaaaat agaattatct aatctcgtat caactgaaat aaatcttcca tttttagctt
     1501 atgatccttc taccggaccc aaaaatctta tagttaatct aacccgttca aaactggaag
     1561 aactcttaag gccaattgtt gaaaaatgta agaatcctat gcaacaagca atccaagatg
     1621 ccaaacttac tcctgcagag attgataaaa ttattatggt tggaggacca actagaatgc
     1681 ctatagttag acaatttgtt gctacaataa tggggaaaga acctgaaaga ggagttgatc
     1741 caatggaagc agtcgctctt ggtgcagcaa tacaaggagc tatcattgca ggagatgttt
     1801 catcagatat tttacttgta gatgttactc cattaacctt agggcttgaa gttttaggag
     1861 gccttaaaga gacactaata gaaagaaata caactattcc taccaaaaaa agtaaggttt
     1921 ttacaactgc ggcagactat caatctgcag ttactattca tgtagtacag ggggaaagac
     1981 ctatggcggc agattgtgtt tcattaggaa catttaacct taccgggatt ccacctgctc
     2041 ctagaggggt tcctcaaatc gaagtaacat ttgatattga tgcaaatgga attctaaacg
     2101 ttactgcaaa ggatttaggc acttctaaag aggcaaaaat aactattaca gcaaccacaa
     2161 aattatctaa agatgaaata gaaaaactaa agaaagaatc agaacagtat tctgaagcag
     2221 atatgaaaaa gaaagaagag gcagaaatta gaaatgaggc tgaaaatctt gtctattctg
     2281 cagaaaaact agttagccaa gatttcaaag acaaaattaa acctgaacaa acagaaaaga
     2341 taaacaaggc agtacaagaa ttaaaagatg tcattgctag taataatatt gaaaatctca
     2401 aagacaaaat taaggaaata aagaatctac ttgctgaaat aagtacagaa atttataaaa
     2461 cagttggagt agcaaatcaa gcaggcggcg gacaaggaac aaatactgag gattttacaa
     2521 atcaaagtga aactaacaga ggagacactt caaatcctgc tgatacatct gatagaagtt
     2581 ctacagataa atctacatcg cctggatcct aacatgtcaa aagataaaaa taatccattt
     2641 tttattttat attaattcat tacgggatta taatttatga ctaacaaacg agattattat
     2701 gaaattttag agatacaaaa gaatgctagt aaagatgaaa ttaaaaatac ttatagaaaa
     2761 ttagcattaa agtaccatcc tgatagaaat aaatcaccag gcgccgaaga aaaattcaaa
     2821 gaaatttctg aagcttatgc agttctttcc gatgatgaaa aaagaaaaaa atatgatact
     2881 tatggacatg taggttcaga ggatgtattt agaggatccg aagcgaactt tgaagagatc
     2941 tttagagata tgggttttgg tggctttgca aaaactattt ttgaacaaat gtttggtggt
     3001 agaggaggaa gagcaggagg attcgaagga tctactgatc cctttagtgg atttagtttc
     3061 aatata
//
LOCUS       DASVEV010000200         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1251223, whole genome shotgun sequence.
ACCESSION   DASVEV010000200 DASVEV010000000
VERSION     DASVEV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251223"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(735..1373)
                     /locus_tag="VF222_09100"
     CDS             complement(735..1373)
                     /locus_tag="VF222_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142621.1"
                     /translation="MSSKEEIKTNQNTDVNKPTFYDKNTTKFNTDNNNFNERTNNTLN
                     QQQDAINKTLDNTLNNVKRTTEEASREIPRYTQRIAEYQEQTIKTIKDIASDFIEAEK
                     QVIGSFQSQVDRNSNGVWDLYNPQRIAENYSVMVNNFTSYLLNTSNLINNALASNMRV
                     YNTALEQTRDNLKACAKTNTNYIQSVSGNSQNRDGNTSNNNNNNINERPTRY"
     gene            complement(2834..2922)
                     /locus_tag="VF222_09105"
     tRNA            complement(join(2834..2864,2880..2922))
                     /locus_tag="VF222_09105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2886..2888),aa:Lys,seq:ctt)
     gene            complement(3007..3528)
                     /locus_tag="VF222_09110"
     CDS             complement(3007..3528)
                     /locus_tag="VF222_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HEX7142622.1"
                     /translation="MERKVCQVCNKRFTRQWNLERHVKDIHHIFEYGGNDMVKQKYEG
                     PSYSYSHTIKNEQFRNSENNTTDMNYYDPPEYHNFTNLYSNKDMYNKRFYPHFQPFPI
                     DKKEDKLTLRDMRRIRLGLQILINFLQRIYPYQFVMMQICWLNYLCCTRKSIQPLRDF
                     YQKNGLMHLWPLY"
     gene            complement(3720..>4274)
                     /locus_tag="VF222_09115"
     CDS             complement(3720..>4274)
                     /locus_tag="VF222_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7142623.1"
                     /translation="EVFTYFARPEHMADQFPENMGLNIIPIEVKNGFGVGTIFRISGD
                     FDGKRLEWDCETIDYIPHRKIVAKMIEGPFKKWQIEVEFEEINEKLTKTGITVDYDMP
                     MGPLGSFIDKVKLKKIAERGIENGLYRVKALLEGTGSIPVYITLDAYRQILKEKERLN
                     CSVSDTILRMFENQKEKPTPPTAS"
BASE COUNT         1441 a          594 c          653 g         1586 t
ORIGIN      
        1 aattatccat tgtaatggta tttttagtcg aatctaccaa atcaataaaa ccgacacaac
       61 tctttattgc atcgccagaa aatctaatcc ctttagaaga aaagtttttc atttagtatt
      121 actccgaaaa ttattgatta aaaatggaaa tttattatta gtataaggta ataaattcaa
      181 tttatttcct cacgtaccac gtttgttata gccttataaa agctaattgg tcaatattta
      241 gaatttagga taattattga ttgtttagat taaccatata gataacttaa attgacaaaa
      301 agtaaaattg ttttgaaaaa attaagatat gtaatcagtt ataaattatg tataaattcg
      361 ataaaaatat gctaaagagg agtaatatcg ataccggttc taaaatagga gtctatttgt
      421 agatagacta aggcattata tttagctttt ctattagaac ttattattta aaatattttg
      481 gaatattgat agatgaattc gttaggaatt tgcaggaggg agtaataccg atagcggttc
      541 taaaagagga atctaatata gagatacaag atgtcttgta tttagctgtt tagtaactta
      601 atttgtttga aataaatgga atagtataaa atgaatctaa gattgacctt tcatgtctta
      661 aaataaaaat aaaataaaat aaagattgat taggtacaat aattcaaaat ttcagcttgt
      721 atactaggtt aaaattaata cctagttggt ctttcgttga tattgttgtt gttgttgtta
      781 ctagtatttc cgtctctatt ttgactatta cctgatactg attgtatgta attggtattt
      841 gtttttgcac aagcctttag attatcacgt gtttgttcta gtgcggtatt gtatactctc
      901 atatttgatg ctagagcatt gtttattaga ttgctagtat ttagcaggta gcttgtaaag
      961 ttatttacca ttacggagta attttcagcg attctttgtg gattatacaa atcccataca
     1021 ccattactat ttctatctac ttgtgattga aaagagccta ttacttgttt ttctgcttca
     1081 ataaagtcag aagcaatatc ttttatagtt ttaatagttt gttcttgata ctcagcaata
     1141 cgttgtgtat atcgtggaat ctctcgtgaa gcttcttcag tagttctttt tacattattt
     1201 aaggtgttgt ctagtgtctt gtttattgca tcttgttgtt gatttaaggt attatttgtt
     1261 ctttcattga aattattgtt gtctgtattg aatttagttg tatttttatc ataaaaagtt
     1321 ggtttattta catctgtatt ttgattagtt tttatttctt ctttgcttga catattaaca
     1381 aatacgttac aagttatata accattgtat taaggactta tatagtgtca tatttgttta
     1441 tttacaatca gtctaactat ttgtataact tgaggtaaag tattcaatta caataatcag
     1501 aagtagagtt atttaggcct tataggtcag tcgggagtaa cgctgatagc agtggaaaga
     1561 taagagtcta atgtatagta ataaagaagg gtgctattca atcattggtt ttgaaattct
     1621 cattctatgt tctgaaataa atctatatct gaataaataa aataatttta tctttcacat
     1681 acgacattat tgccaaaaaa aagagttaga tattaataaa ttttcagaaa tttattaaac
     1741 gctcattaaa agtttgaagt taattaatta tactatatca tagcgtaata acgttcacta
     1801 atatataaca ttcaactaga acagtaatcc agtatatagt ccttatagcc taacatagaa
     1861 tacgataaag tatacaactt ttactacaag attatttaaa ggtcgccaat gttaaataag
     1921 ttgttaattg ttatgcataa cgaagaagaa tgatggtgga atagaattcc atacgaatta
     1981 aaatgtaaag gagaaataga atgttataat aaaataaatg aacttcaccc taaatatccc
     2041 gctataattt ataattatgc tctaaaatat aaacaaaagg ttaaagaata tttaggtaaa
     2101 agtaaatcat taaaaaaaat tgatggatta taataaataa acaatgactt gtcactatta
     2161 gtaattaata atcgctttcg agttgccttc tgcagataag aggcttattt cggccagtcc
     2221 cgtatttggc ctttcctctt taccgtaata gtgtctcggg gaacgggacc gaaacccggt
     2281 acaccatttg ctgcagaagg caacttttat tgaatttctt tctataaaac aatttattat
     2341 cgtaaagcca tagtcttcta ctaactaaac acgtatagaa ggctatggtg acgttagtct
     2401 ttggttttgg agtgttttct aatttatccc ataatattta ataatttgag ataattttat
     2461 taattaatag caactgttag tataagaaaa aaattaattg aattttaaaa taatttcaat
     2521 agatttattt tgatagagta tgatgaactg cttttgtcag taaaaatata acaaatccta
     2581 atgaaactga tgtaataata taaggtatta ttggggggat tttcacttaa taagataaat
     2641 aacaattaaa atataattga agtgcttagg taaacttgct aagatagtat tactaaatta
     2701 aattagttaa tgtttattat gattacccgt tgctaatttt tcttattaaa agagtactga
     2761 aaattggttg aatgtttatg ttagttaatt tctctataac attatttcgt tactagtaat
     2821 agtaacttaa tttcgggttc ggtgggattc ggacccacga cattttaacc ctagatagac
     2881 tggttaagag ccagacgctc tacctggctg agctacgaac ccccttttta tatctcattt
     2941 tcgagaaaat aaattctttt tcattgatta aatgacctaa atgaattgcc gtgatagtag
     3001 gataggtcaa tataatggcc aaagatgcat taaaccgttc ttttgataaa aatccctcaa
     3061 cggttgaatg ctttttctag tacagcagag ataatttaac caacagatct gcatcataac
     3121 aaattgatac ggataaattc gttggagaaa atttattaaa atttgtaagc ccagtcgtat
     3181 tcttctcata tctcgtagtg ttaacttgtc ctcctttttg tctattggaa atggttgaaa
     3241 atgtggataa aaccttttgt tatacatatc tttgtttgaa taaagatttg taaaattgtg
     3301 atattcagga ggatcgtagt aattcatatc agttgtattg ttttcagaat tcctaaactg
     3361 ttcgttctta attgtgtggg aatatgaata agaaggacct tcatattttt gtttgaccat
     3421 gtcatttccc ccatactcaa aaatgtggtg aatatccttc acatgtcttt ctaggttcca
     3481 ttgcctggta aacctcttat tacatacctg gcatactttc ctttccatat tatgaatatg
     3541 atcttggcat ttaaataaag aaaacaatta tgtcagagta actcgagtaa ctcgaaataa
     3601 ctcattagag ggatgagaaa gaccgataaa gtttaaagaa ttatttcaaa taaacgataa
     3661 atgcatatat aataagtgta gagcttaaaa atgggattga aactttgttc ttcttgatgt
     3721 caagaagcag ttggaggtgt aggtttctct ttctgattct caaacatcct gagaatagta
     3781 tctgaaaccg aacagtttaa tctttctttt tccttcaata tctgtctata cgcgtctaaa
     3841 gttatataga caggaataga accagttcct tcaagaagag ctttcactct ataaagtcca
     3901 ttttcgattc ctctttcagc tattttcttt aactttacct tgtcgataaa acttcctaat
     3961 ggacccattg gcatgtcata atcaacagta atgccagtct ttgttaattt ttcatttatt
     4021 tcttcaaatt ctacttcaat ctgccatttt ttgaaaggtc cctcaatcat ttttgctaca
     4081 atttttctat gaggaatata atcaatagtt tcacaatccc attctaatct ctttccatcg
     4141 aaatctccac ttattcgaaa tatagtccct acaccaaaac catttttaac ctcaatcgga
     4201 ataatgttaa gacccatatt ttctggaaat tgatcagcca tatgttcagg tcttgcaaaa
     4261 taagtaaaaa cttc
//
LOCUS       DASVEV010000201         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1057247, whole genome shotgun sequence.
ACCESSION   DASVEV010000201 DASVEV010000000
VERSION     DASVEV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057247"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF222_09120"
     CDS             <1..345
                     /locus_tag="VF222_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142624.1"
                     /translation="QAINTFQSITNNFVELQKNLLETYQSVISRFLDVTSKSYRNNST
                     SPQKYTDIYNKTNQTIKDNTINATNQINDALLGSTETFNKSVEIAQKYYNESIQNYFN
                     FLKKIERSSPNQ"
     gene            1184..1342
                     /locus_tag="VF222_09125"
     CDS             1184..1342
                     /locus_tag="VF222_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142625.1"
                     /translation="MIRITGKMTVKPLSPLYIWVHIISSAVANGVPGFGPVLRVINRE
                     RPNTGQVR"
     gene            complement(1527..1829)
                     /locus_tag="VF222_09130"
     CDS             complement(1527..1829)
                     /locus_tag="VF222_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142626.1"
                     /translation="MNNILNSPTTLSEVIDNKIKIFANDIDKSDNFNPNQVDVLLRTS
                     YLLTKLSSIKSELQSVENEIINIHKSDYTKENRVFNKILKEEINTLNWTLQKIKNL"
     gene            2257..2547
                     /locus_tag="VF222_09135"
     CDS             2257..2547
                     /locus_tag="VF222_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142627.1"
                     /translation="MIFNVNNNNNKHEQNFVKIDKCIFEINNVITKICKEQVMKTIDD
                     RKVDIITEGDIKCVYDTAIISVIHKFITISNECEKIYLKECAAAGIEAGKLR"
BASE COUNT         1032 a          353 c          371 g          960 t
ORIGIN      
        1 caagccatta atacattcca atcaatcaca aacaactttg tagaattgca aaagaatctt
       61 cttgaaactt atcaatcagt aatatcaaga tttttggatg ttacatctaa atcttataga
      121 aataattcta catctccaca aaagtataca gatatttaca acaagaccaa ccaaactatt
      181 aaagataata caattaatgc tacaaaccaa ataaatgatg ctctattggg atctacagaa
      241 acttttaata aatcagtaga aattgcacaa aaatactata atgaatcaat tcagaactac
      301 tttaactttc tcaagaaaat agaaagatct tctcctaacc aatagataca tttttttatt
      361 gttttaattt tgaaatacaa ccttattaga ttgtctaact acttgttata ttacaaggat
      421 taaatgatta ttgatgatca attagaaaat aagaatcaag ataacttcat ataaaatacc
      481 aaatattaca ttattaacag atcttgaata tatattcaat taagaaagtt aaagaaatag
      541 taaatagaaa tgtttataga tcataataac ctaaatattt tgaatcccat tttagaatca
      601 atatcacaat ggaaacctct gagcccgtta tgtgtatatg gattcgtata atttagaaag
      661 aaaatcaatt ttattagaag agggataaat ctaagatggt agcaagtctg agaatggttg
      721 tttaagagaa aaacttttca ttattattag tatatatcat ataaacaaag aaaagtccta
      781 aattatataa ttcattacaa ttaacaaaat atctaatatg aataaaataa ttattctact
      841 atttcatttg tttcattaat aataatcaca tttttagcat tgtcaatctc tttaatttta
      901 ataatgtcgt ggataaaatt ttataacacg cagagttcag agcaggtcta cattttaatt
      961 tcgctaccag ttttttaatt agtaaaaata actactaaag gttaatcata tatcaaaaaa
     1021 gaaaatcaat ttctatgtga aaatctgggt ataaacaaag aaaatatata cccattttat
     1081 aatattttga ttactttaga tttttggtta ttttattatc taaaaaatat ctttgttcag
     1141 caatatttgt tgtttatacc taagtaatta cgtaactttc tggatgatta ggattacagg
     1201 caaaatgacc gtcaaacctc tgagcccatt gtatatatgg gttcatataa tatcatctgc
     1261 tgtagcaaat ggtgtaccgg gtttcggtcc cgttctccga gtgataaata gggaaaggcc
     1321 aaatacggga caggtaagat gattaaacca taactaacat tctctagttg ttggttgtat
     1381 ttgtaatccc aaattaaagt tactgataga aaattaccgt tagcctaaag tggaattgtg
     1441 gttctctccc tcattgtact tgctggctgg aaaaattcaa ggtaagagaa aagttcaggt
     1501 gttccagcag agtatgtttg taccctctaa agatttttaa ttttttgtag agtccaattt
     1561 agtgtattta tttcttcttt gaggatttta ttaaatactc tattctcttt tgtatagtct
     1621 gatttatgga tgttgataat ttcattttca actgattgaa gttccgattt aattgaagat
     1681 aattttgtta ataaatatga tgttctcaac aaaacatcaa cttgatttgg attaaaatta
     1741 tcagacttat caatatcatt tgcaaatatt tttattttat tatctataac ttcagatagc
     1801 gtagtagggg aatttaaaat attattcata gttatcatat catatagttt agcgagaacg
     1861 ttattatact tttattaaat atctatagat tattttcgcc ctccataaca atgatggtca
     1921 aacaattgtc aagttatcat attctggaat agttattagt tattatatat taattacaca
     1981 atttaatttg taaaaaaacc ccttaaatat tctctctttt tactataaca ttggattctt
     2041 attttagcac cgctatcagt attactccct atcgaccctt tttatcaatc ttcctataca
     2101 tttatttttg tatattactt caacaataag ataaactatg tcaagtaaaa ttataaggtt
     2161 atagagattg tacaaaataa taaaagtaaa aggatagaat ctattaacta actattagat
     2221 tagattggat tagaaggata aaaaggagga aataaaatga tttttaatgt taataacaat
     2281 aataacaaac atgaacaaaa ttttgtaaaa atagataaat gtatttttga gattaataat
     2341 gtaattacca aaatttgtaa agaacaagtc atgaaaacaa ttgatgatag aaaagttgac
     2401 ataattacag aaggtgatat caaatgtgtt tatgatacgg ctataatctc agtaattcac
     2461 aaattcataa ctatatctaa cgaatgtgag aaaatctatt tgaaagaatg tgctgcagca
     2521 ggaatcgaag caggtaaatt aagataatca agttacaaat actaataatt atatctaatg
     2581 aggtatggtg tgtaagggaa tatgttcggg ccataaagca attaggccct caaatggtca
     2641 gcgttatttg ataggacaaa aacgctgtca agtttgtcaa attttcatca attgggaagg
     2701 tgtattttgc ccttgt
//
LOCUS       DASVEV010000202         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1251625, whole genome shotgun sequence.
ACCESSION   DASVEV010000202 DASVEV010000000
VERSION     DASVEV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251625"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..337)
                     /locus_tag="VF222_09140"
     CDS             complement(8..337)
                     /locus_tag="VF222_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142628.1"
                     /translation="MINVILTVLADEELRGIFNMIKEKPVSAQEILRYTKLPHTSFYR
                     KINWMLDNNLVIIDSVQETKDGKKYSLFRAIFDDITIKLQRDVITIEAIPTVDLAELT
                     ANEFFEI"
     gene            complement(790..1650)
                     /locus_tag="VF222_09145"
     CDS             complement(790..1650)
                     /locus_tag="VF222_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7142629.1"
                     /translation="MQRIHSTSNSINGLLDNIDISKIIPFRYSYRRNINEDLAELSYS
                     IKEKGLLHPIIVRMIKDGEYEIVAGTRRYNACKMLGWRKILSNVVELEDKAAFEVSLI
                     ENIHSKNIHPLEEARAFQDYVTNYGWGGISDLAPRLGKSISYVDKRIRLLDLPPNVVE
                     SLFTGSLSVSIGDELLPLKEKEQQSMLAELIKKRKLSSRQVRELKTEMKNSDNPYDVL
                     NFQTKLIDIDAKIQKSFDKAIIAIRVAMNNLASVIEDVDNWTVYEILLQHKNMLHNQI
                     DILFKEKKKI"
     gene            2897..3205
                     /locus_tag="VF222_09150"
     CDS             2897..3205
                     /locus_tag="VF222_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142630.1"
                     /translation="MENNFILLLLALAIFSINAIFRQNFRSFQMTIIIFILIMIFGEV
                     VINFTDINDSINKNHAMELKVQIQFVALVFLLTVVWFRYYKILKSRRKLAETIESILK
                     "
     gene            complement(3413..4474)
                     /locus_tag="VF222_09155"
     CDS             complement(3413..4474)
                     /locus_tag="VF222_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7142631.1"
                     /translation="MLKYSCIVFAHAILLSFESIFAEFIQNSLQVSVITIISISLPIS
                     GGILLLIHFTSKRNNNRNGNDDNNNNNNIILINIFKNSIFKNFRQLFPAAIFLSTGIF
                     TWYDAASRIGASKDGLIAGALEITIVLFLAHLILKERLTRTQIFGVFLAIIGFFITIS
                     LGNNTGSSSINYFSLGDFESIISAICFASSYLFTAKLVKNDSPIKITGLLLFLSGLAF
                     IVLSFVIFTVTKNTIFSFEGLNIEELWILFLFSFVPLSSALFYNIGMKKLGASITSIL
                     ASSTIILTIIFQVLLNMMGHAMILPLNISMAILGGSIGIIGIFIIHLPSDKLFVILNG
                     KFKNKIYIKNHSLEVNNKI"
     gene            4724..>5102
                     /locus_tag="VF222_09160"
     CDS             4724..>5102
                     /locus_tag="VF222_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142632.1"
                     /translation="MDQCITSIREEIMKVVMNETTLKILNQIIDNNNNNNNDKDGNTL
                     SITKIGLTRKQFYTKLNELTTRGLVKKEKGKYYLTTFGKIVYDLTFEYKCKLDSVIED
                     CWKFKALDLLDISDELTNDEKQKI"
BASE COUNT         1898 a          639 c          705 g         1860 t
ORIGIN      
        1 tattatatca tatctcaaaa aattcatttg ctgttaattc agctaaatca actgttggga
       61 tagcttctat agttataacg tctctttgta attttatagt aatatcatca aaaatagctc
      121 taaacagaga atatttttta ccatcttttg tttcttgtac actatcaatg atgactaagt
      181 tgttgtccag catccaattt atttttctat aaaacgaagt atgagggagt ttagtatatc
      241 ttaatatctc ttgtgcagat actggttttt ccttaatcat gttaaagata cctcttaact
      301 cttcatctgc taatactgtt aggatgacat taatcatttt tttatccctt atgaacactt
      361 ttaacaagaa ttttcatctt agtacatatt atactttgtt ttattgaaga atattcccaa
      421 atgtgggaat tattatgtag tgaaaaaagt catataaatc cattcattga catttaagta
      481 aactgcctta catttttgtt aagagtatga tagaaagttg tagtttgtgt atgtgaatac
      541 tgttatctga ttagttaaag caattattca acataatctt gtttgtagaa atatacaaaa
      601 gattatacca ctttgtccat attttttctc ttgatcccat ttttggtact ggatttatga
      661 attagatatt ttttatcttt aatttattcg atcttaatag agtttctatt atttcatcaa
      721 tttttttagg cgccggaaaa tttttcgagt aaaagtgtaa taaaaaatca aatacaaata
      781 taaaatattt tagatctttt tcttttcctt gaaaaggata tctatctgat tgtgtagcat
      841 attcttgtgc tgcaaaagga tttcatagac agtccaatta tctacgtcct caataactga
      901 agccaaattg ttcatggcga ctcttatggc gataatagct ttatcaaatg atttttgtat
      961 ttttgcatct atatcaatta atttagtttg aaagtttaag acatcgtagg ggttgtcaga
     1021 gtttttcatt tcagtcttta actctctgac ttgtctagac gatagttttc gtttttttat
     1081 caactcagct aacattgatt gttgttcttt ttcctttaat ggtagtaatt catctccgat
     1141 acttacactt aaactacctg taaaaagaga ttcaacaaca tttggtggta gatcaagtaa
     1201 tcttattcgt ttatctacat aactgatact ctttcctaac ctgggggcta aatcagatat
     1261 gcctccccac ccataattgg tgacatagtc ttggaatgca cgagcttctt ctaatggatg
     1321 gatattttta gaatgaatat tttctattaa tgatacctcg aatgcagctt tatcttctag
     1381 ttctactaca ttagatagaa tttttctcca gccaagcatt ttacaggcat tataccttct
     1441 ggtacctgca actatttcat attctccatc tttaatcatt ctaacaataa taggatgtaa
     1501 aagtcctttt tcctttatag aataggataa ttcagctaga tcttcattga tatttcttct
     1561 gtaggaatat ctaaatggta taatttttga aatatcaatg ttatctaaca gtccatttat
     1621 gctgtttgaa gtagaatgga ttctttgcat atctcgtatt attgtataaa gagtatataa
     1681 ccttttttca atttacctta ttatagtcaa ttattcccag ctatggtact aaattattaa
     1741 taaaaatact ttcgtttatt atgtatagtc aattaatccc atttttggga ctcggtaagg
     1801 aagtatattt ctattactaa agaaactaaa catttacgag aaggtcatga atttaaaaag
     1861 tagaaaatac tcctaacttt agagtagcaa cataacagta ggttgagata ttaaaataaa
     1921 tatataaaaa aacaagaata ctattcaggg tagaataaaa gaattcaaag tagtactatg
     1981 tgataaataa atagaagaaa tattgtataa atcaaaatat aagaaaggaa gatagcaaga
     2041 agtaacggat tgaaaaagta tagtacatga tattgtttta gtccttttta attatagaat
     2101 caaaaaaagg gtaataatct gtctatagta tagaaaactc atacagggcg attaggaaag
     2161 taatagtgga atttgaaaat ttgattaaat aagtgaaaga tgtatactga aaaaatgcct
     2221 aataatgatt ttaaaagagt aaaaatagtt ttagatattg tagaggacta ttggagaata
     2281 tgttatcata tatcgagaat ggagaaatac tttagtgcca tcagaataga atataggata
     2341 aaaaaagtaa actgaatgca atatagcata tttggtttag gagtctaaaa atagtttaac
     2401 taaatataat tgcaagttac aagtttgatt caatttcttt gtgccatagt tcagcaaata
     2461 ctactcattt agttttttta ctttccattt aaggaaaaaa aagtatacta atatccaaaa
     2521 atggagatat taataaagaa ataactaaaa gagtttaatt ctaccagaat cttttacatt
     2581 agtcattgtc attcaacata caacttatta caatgattat tccattttta attatagtca
     2641 ttccaatttg gatgataaga ggaactcatg ggatattaag agttatatca attctattta
     2701 caggattcat tgggttttta tggatttatt accttattaa ttttctcaac ttttctgatt
     2761 tttctgaatc gataattaag cctatttact tatcttttct tattgcaact ggattgttga
     2821 tattaattac acaaaagaaa aaaaatattg aatataaatt tttcaaaaat acaattaata
     2881 aacaatgatt gagatggtgg aaaacaattt catactttta ttgcttgcat tagctatatt
     2941 cagtattaat gcaatttttc gacaaaattt tcgatctttc cagatgacta taataatttt
     3001 tattttaata atgatttttg gagaagtggt aattaatttt acagatataa atgatagtat
     3061 taataaaaat cacgcaatgg aattgaaagt acaaatacaa tttgtagcct tggtattttt
     3121 gttaacggta gtctggttta gatactataa aattttgaaa tctagaagaa aactagcaga
     3181 aactattgaa tcaatattaa aatagatatt taatatttta ataattgtct ttcaatttcc
     3241 atcccaatca acatttttaa ttttctcttg tttaatttga ttaattcgat atcaataagg
     3301 atatgaccat attctaatct attcttgata ggttgtagag gttcccttgg acattaaaag
     3361 aataatgaga ttgcatgatg atgataatat tacagaaaag attatagaat catcatattt
     3421 tgttattcac ttccaggcta tgatttttta tatatatttt atttttaaat ttaccattta
     3481 aaataacaaa taatttatct gaaggaagat gtataataaa aattcctatt attcctattg
     3541 aacctcctaa tatcgccatt gaaatattta aaggtaaaat catagcatgg cccatcatat
     3601 taagtaaaac ctgaaagata atggtaagta ttattgtaga agaagccaga atagaagtta
     3661 tagaagcacc taattttttc atgccgatgt tataaaataa tgcagaagat aatggtacaa
     3721 aggagaacaa aaataatatc caaagttctt ctatatttaa accttcaaaa ctgaatatag
     3781 tattttttgt aactgtaaat attacaaatg atagtactat aaatgctaaa cctgataaaa
     3841 ataataacag acctgtgatt ttgataggag aatcattttt aactagttta gcagtaaata
     3901 aatatgaaga tgcaaaacaa atagcagata ttattgattc aaaatctcct aaactaaaat
     3961 agttgattga tgatgatcct gtgttgttac ctagagaaat ggttatgaaa aatcctatta
     4021 ttgctagaaa aactccaaat atctgcgttc ttgttagcct ctcttttaat atcaaatgtg
     4081 ccaaaaataa tactatagtt atctctagag cccctgcaat gagaccatct tttgaagcac
     4141 ctattctgct agcagcgtca taccatgtaa aaataccagt agataagaat atagctgcag
     4201 gaaataactg tctaaaattc ttaaagatgg aatttttaaa aatatttata agaataatat
     4261 tattattatt attattatca tcattgccat ttctattatt atttcttttt gatgtaaaat
     4321 gaataagcaa taagattcct ccagaaattg gaagtgatat gcttataata gttattacag
     4381 aaacttgtaa agaattttga ataaattctg caaagatgga ttcgaaggag agcaaaattg
     4441 catgagcaaa aactatacat gagtatttta gcatctcaat tatttctgat tatgtatgta
     4501 ttgccagagt ataaataact acgtaatatt tcgccattta aaattataaa tttgataaaa
     4561 atatctaaaa atgagaatat tattgaaata tcatgataaa ttttgcagtg tttatatatc
     4621 atatattaaa ttttaatccc agttttggga ataagtatct aattgaatag gtaaaaattc
     4681 aataaattta tattctataa tgatattgtt tacctcaaat ataatggatc aatgtattac
     4741 cagcataaga gaagaaataa tgaaagtggt aatgaatgaa actacattaa aaatccttaa
     4801 tcaaataata gataataata acaataacaa taatgataaa gatggaaata cattgtcaat
     4861 tacaaaaata ggcttaacca gaaagcaatt ctataccaag ttaaatgaat taacaactag
     4921 aggacttgta aaaaaagaaa aaggaaaata ctatctcact acatttggaa aaatagttta
     4981 tgatttaact tttgagtaca aatgtaaatt agattcagtt atagaggatt gttggaaatt
     5041 caaagctcta gatttgttag atatatcaga tgaattaacc aatgatgaaa aacaaaaaat
     5101 aa
//
LOCUS       DASVEV010000203         6755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_670536, whole genome shotgun sequence.
ACCESSION   DASVEV010000203 DASVEV010000000
VERSION     DASVEV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670536"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..90)
                     /locus_tag="VF222_09165"
     tRNA            complement(join(2..37,53..90))
                     /locus_tag="VF222_09165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Lys,seq:ttt)
     gene            384..1283
                     /gene="uvsE"
                     /locus_tag="VF222_09170"
     CDS             384..1283
                     /gene="uvsE"
                     /locus_tag="VF222_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX7142633.1"
                     /translation="MKIGYPCINHSIGKKTVSTFRLSSYTEEKFIQCVNYNLATLVEI
                     LKFNIANNFMFFRISSDMIPFASHPICKFDWKEHFKSDLIKIGQLINDHNIRISMHPD
                     QFVLINSKSQEIVNNSIKELEYHTDLLDLMTLDYSAKVQIHIGGVYGDKEKSKKQFIS
                     NYKTLLSANIKKRLVIENDDHLYNLKDCIEIHEYIGIPILFDVFHHICFHNNLPLHIA
                     LQISNNTWNHSKDGIMMIDYSNQELNQRKGKHSHTLDIKQFEKFILSVSNLDFDIILE
                     IKDKEKSAKKAIEVINKINRNLE"
     gene            complement(1526..2296)
                     /locus_tag="VF222_09175"
     CDS             complement(1526..2296)
                     /locus_tag="VF222_09175"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7142634.1"
                     /translation="MKENNKKNETIYNTIARFIKQEIEKRESQGVVIGMSGGIDSSLT
                     TVLAVKALGNHQVLGLILPDSSLTPKTDTKHAIDLAKSLKIKYQVIELNKIKKQIVNG
                     LPKNKMARANLLVRLRMSLLYYYATVMNRLVLGTGDKSEMMLGYFTKYGDGGADLFPI
                     ADLYKTEVRSLAKYLGIPAKIIDKKSSARLWKGQTAEKEIGIKYEEIDKILVHIEYNN
                     TPLSSNSAKLHKDNVSKIKSMVEKNEHKQDMPPICKLQ"
     gene            2863..3246
                     /locus_tag="VF222_09180"
     CDS             2863..3246
                     /locus_tag="VF222_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142635.1"
                     /translation="MKNILIVDDDVNTSLKYKKWLEDEGFNLILINDPLLAELNFKSD
                     NYDLILIGFKMSVIDGFNLYNKINEISKKVEHTPKEFRVCFMTSSVINYKVLAEIHPE
                     IGEECYVSKEVPKEVFIKHVYSLIS"
     gene            3581..4201
                     /locus_tag="VF222_09185"
     CDS             3581..4201
                     /locus_tag="VF222_09185"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX7142636.1"
                     /translation="MKNKTKIIIGIIVTAIVIPLGIYTISPLFINTEINEPLPSLANE
                     KSTTDFAKFMAMTEDEKLDAAKNMVEEEEDMIMKMATKQHTTINETMTMNISQSAQNK
                     ALIGNFIGIDDGIHNAKGLAKILILSNGSQILRLENFKSTNGPDVHLYLSNNEQANDF
                     IDLGRLKANNGNQNYKIPMNIDFNHYGYVLIWCKPFSVLFGSAQLN"
     gene            complement(4447..4698)
                     /locus_tag="VF222_09190"
     CDS             complement(4447..4698)
                     /locus_tag="VF222_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142637.1"
                     /translation="MNQISIYIANNLNLINTIAIKSNNSRYNTKKYNNNNINQALYLS
                     FNWSSKLGLVLQSWITGAGGGQYGSVGFIVDCPIVFRFL"
     gene            4737..5273
                     /locus_tag="VF222_09195"
     CDS             4737..5273
                     /locus_tag="VF222_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7142638.1"
                     /translation="MNNNITSVLTITILIAVTITMVSALTFSNHSVFAQKEKFNAKLS
                     GQEEVPPVQTTASGMAWFKPMQDKVWFKVNITDMQGVTQAHIHTGKVGENGPVVVTLY
                     KSDTPQPINGKLSYGNITANVLEGPMKGKQLSDLATAMSNGSTYVNVHTEKHPNGEIR
                     GQIMMANSTSDKMMMMDK"
     gene            complement(6052..6429)
                     /locus_tag="VF222_09200"
     CDS             complement(6052..6429)
                     /locus_tag="VF222_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142639.1"
                     /translation="MYIVNILNLSIIGSILLTFGILSNPTLAFSQQEPSSVVITLGAA
                     DEGNFEPFTPRALNVMPGSMVSWTNEDSTQHTVTAEGTGAPLFDSGPIPPGMTWDNTF
                     DSTGEFGYHCLIHPWMTGRVMVG"
BASE COUNT         2524 a          875 c          917 g         2439 t
ORIGIN      
        1 acgggcccgt cgggagttga acccgagaca gccggatttc ttccattaga gttaaaagtc
       61 cggtactcta cctggctgag ctacgggccc ataaggtaat tacgattcag tgtagtgtgt
      121 attattcact ataaatatta acctgatcat tgacttaatt aaataatcca tttaaaattg
      181 acagtgttag taaaaaaaaa taaaataaac ctttttctta caaaattcaa gtatggttat
      241 tatttaattt tatttgttaa aatttatgaa ggtagtatct acaaatatac gctttgtatt
      301 ttatagcccc tttctaattc ttgaaactat tttatatttt actttacata attaaaagag
      361 ttctgagtga ggttaaagaa aatatgaaaa taggatatcc ttgtataaat cactcaattg
      421 gaaaaaaaac tgtatcaaca tttcgactat cctcgtatac ggaagaaaaa tttattcaat
      481 gtgtaaatta taaccttgct actcttgtag aaatcttaaa atttaatata gcaaataact
      541 ttatgttctt tcgaataagt tcagatatga ttccttttgc atctcatccg atttgtaaat
      601 ttgattggaa agaacatttt aaatccgatc ttattaaaat tggacaatta ataaatgacc
      661 acaatataag aatatctatg catccagatc aatttgttct tattaattct aaaagtcaag
      721 agattgttaa taatagtatt aaagaacttg aatatcatac agatcttttg gatttaatga
      781 cactagatta ttcagcaaag gtccagatac atattggagg ggtttatgga gataaagaaa
      841 agtctaagaa acaatttatt tctaattata aaactttact atcagcaaat ataaaaaaaa
      901 gattagttat tgaaaatgat gatcatctct acaatttaaa agactgtatt gaaatacatg
      961 aatatattgg gataccaatt cttttcgatg tatttcatca tatatgtttt cacaataatt
     1021 tacctctaca tattgctcta caaatatcta ataatacatg gaatcatagt aaagatggta
     1081 taatgatgat tgattatagc aatcaagaac ttaatcaaag aaaagggaaa cattctcaca
     1141 ctttagatat aaaacaattc gagaaattta ttctctcagt ttctaatcta gattttgata
     1201 ttattttaga aataaaagac aaagaaaaaa gtgctaaaaa agcaattgaa gtaataaaca
     1261 aaattaatcg aaatttagag taacagttaa tttgatttgt tttataatta tcaattatat
     1321 tgataacttc tattttttat tttttctata aaaattgatt acgatcataa atagtagatg
     1381 tatttttaac tatccatatt taattttaat atacttttta ttggattaag atatcatgca
     1441 attgttaaaa ttttaattaa ttaaatcgtg atgaagaaga atcctttgct acattgtata
     1501 gactgctgct aataaatgtc aaccattatt gtaatttaca aattggaggc atatcttgtt
     1561 tatgttcatt tttttcaacc atagacttta tcttagaaac attatcctta tgtaatttag
     1621 cagagttaga tgataaagga gtattattat attctatatg tactagtatt ttatctattt
     1681 cttcatattt gatacctatt tctttctctg cagtttgacc tttccataag cgagcactac
     1741 ttttcttatc tattattttg gcaggaattc ccaaatattt cgcaagagat cttacttcag
     1801 ttttataaag atcagcaata ggaaaaaggt ctgctcctcc atctccatat ttagtaaaat
     1861 atcccaacat catttcactt ttatcacctg ttcctaacac caatcgattc attactgttg
     1921 cataatagta tagtaatgac attcttaatc taactaaaag attcgctctt gccattttat
     1981 ttttaggaag accatttact atctgtttct ttatcttgtt taattctatt acttggtatt
     2041 ttatttttaa ggatttggct aaatcaatag catgtttagt atcagttttt ggagttagag
     2101 acgaatcagg aagtattaat cctaatactt ggtggtttcc taatgcttta actgctaata
     2161 ctgtagttaa agaagaatct attcctccac tcataccaat tactacgcct tgagattctc
     2221 ttttttcaat ttcttgcttt atgaatcttg ctatagtatt ataaatagtt tcattttttt
     2281 tattattttc tttcatgagg ataaatcaaa tcagaataaa tctattgata taatttaact
     2341 ttttgtcaaa gcttttattt gttttaatta aaatttagga attgcatttt gatttcaaaa
     2401 atagagatta tattttatag tgataaagaa tggtttatta aattcatacc aataaccaat
     2461 actaatattt tgcgtcattt ttttagttac tatatttaga atactattca aaataaagaa
     2521 ttcattgtat gaaataacct ctgaaagctt tcttctaggc atatcaatgt tataaaaagg
     2581 taataatttg ttattttttc tagagtctat ataaaggtct tttttaatcg gaattactca
     2641 gcattatata taaaggatca tttcaaaagt tagaacaaat caaagaatta tttttagata
     2701 ttgatatatt tgtttaatta tctaccgtgc cctggcatga cattcataac acttaacatt
     2761 tattattctt aatgacaaaa tattttaaag attttacaaa tctttaaagt aatactaatt
     2821 ttataagaaa ttattattaa taagttcggg ttaattataa tattgaaaaa tattttaata
     2881 gtggacgatg atgttaatac ctctttaaaa tataaaaaat ggctagaaga tgaaggattt
     2941 aatttaatat tgataaatga tcctttatta gcagaattaa actttaaatc tgataattat
     3001 gaccttattc ttattggttt taaaatgtcc gtaatcgatg gatttaatct ttataataaa
     3061 ataaatgaaa tttcgaaaaa agtagaacat acaccaaaag aatttagggt ctgtttcatg
     3121 acttcatctg taataaatta taaagtgtta gccgaaatcc atcctgaaat aggagaagag
     3181 tgttatgttt caaaagaagt tcctaaagaa gtttttataa aacatgtata ttctttaatt
     3241 tcataaattc tcattttatt tataaaataa atagattctt aatacaattt aactaatcat
     3301 atggaattta gcctttccac aaagaattga acatatttcg atcttatcaa tttaaattct
     3361 attttcctat tatatttaca aaatatcgaa aatattttca aatggatatt tggataaata
     3421 attattaagt ttgaatcttt taataaatat gaaaatttac tttaactgat tattctcata
     3481 atattcatgt taaaacaatc taaataaata tatatataaa taaattatgt taaataaata
     3541 aatttgtttt tatattataa tataactagg ataaaattaa ttgaagaata aaacaaaaat
     3601 aataatagga attattgtta cagcaatagt aattcctctt ggaatttata ctatttcacc
     3661 tttattcata aataccgaaa taaatgaacc tcttccttca cttgcaaatg aaaaatcaac
     3721 aacagatttt gcgaagttta tggcaatgac agaggatgaa aaacttgatg ctgcaaaaaa
     3781 tatggttgaa gaagaagaag atatgataat gaaaatggca acaaagcaac atactactat
     3841 aaacgaaact atgactatga atattagcca aagtgcacaa aataaagctt tgataggtaa
     3901 ttttataggc attgatgatg ggatacataa tgcaaaggga ttagcaaaaa tattgatctt
     3961 atcaaatgga tctcaaatac tacgactcga aaacttcaaa tctactaatg gacctgatgt
     4021 tcatctttat ttgtctaata atgaacaggc aaatgatttc atagatcttg gtaggctcaa
     4081 ggctaacaat ggtaatcaga attacaaaat acctatgaac atagatttta atcattatgg
     4141 atatgtttta atatggtgta agcctttttc ggtattattt ggtagtgctc aattgaatta
     4201 atcataggat tggatatata ttcgatcaaa taaaatagaa taagaaaaaa caattaattc
     4261 cttcctttat aagcttagat ttattttaat aatatcttta gaatttaact atgaaagtta
     4321 tacaatttcc tataaaatta ttttattact aaaaaatgaa tataaaaaaa aattttgtat
     4381 acaaaacaat aataaataaa ctataataaa atgatatgaa ttgtacttta atgaaaaaat
     4441 ttgttgctat aagaaacgga atactatagg acagtctaca ataaatccta ctgaaccgta
     4501 ttgaccgcca ccagcacccg ttatccaact ttgtagaact aacccgagtt tggagctcca
     4561 attaaacgag aggtaaagtg cttgattaat attgttatta ttatattttt tggtattata
     4621 acgactgttg ttagatttta tagcaattgt gttaattaga ttcaaattgt tagcaatata
     4681 aatagaaatt tgattcatgt attaaatatg atgtagtaca agcatagata atgagtttga
     4741 ataacaatat aacatctgtt ttaacaatta ctattcttat tgctgtgaca ataactatgg
     4801 taagtgcact cacattctca aatcatagtg tatttgcaca aaaagaaaaa ttcaatgcaa
     4861 aactttcagg tcaggaagaa gttccaccag tccaaactac tgcatctggt atggcatggt
     4921 ttaaaccaat gcaagataag gtatggttta aagttaatat aactgatatg caaggagtca
     4981 cacaggctca tatacatact ggaaaagtag gtgagaatgg tcctgttgtt gtaacattat
     5041 acaaatcgga cactccacaa ccaataaatg gtaagttatc atacggaaac ataactgcaa
     5101 atgtgttaga aggaccaatg aaaggtaaac aactatctga cctagctact gcaatgtcaa
     5161 atggaagtac atatgtgaat gtccatactg agaaacatcc taacggcgaa attcgtggac
     5221 aaataatgat ggcaaattcg actagtgata aaatgatgat gatggataaa tgataaacaa
     5281 ataaattttt ttatttttaa ttttattttt tttaactttg acctttgaat ttgaatttgc
     5341 taattatact ctatatcaat caaataacta ttgaaaatga tgattatagt tattttgata
     5401 atattcgatg cttgattcga tgcctgatcc agatataatt tgattgttat ggtttctttt
     5461 gctggaacga gaggagatga taatagaatt aacattcttg atctactact aaaaatccat
     5521 taaatatcaa taagatttca gaagaattac aattagatta taaagcaatt caacattatt
     5581 tacatatttt agagaaaata atttgataac tagaatgggc gataagtagg gaatttttta
     5641 ttttatttcc aactatatgg aaaaaaatat tgaacctatc ataaaataaa gaagaaatta
     5701 gctcctcact tatatggtga tttacaggca taaataatat acaatcaatt tgattagctt
     5761 atactcataa ttattgtaaa attcataagc ctcatacatt ctttctaatt tcaaaaattg
     5821 ggaccataat ttgtgagctt gttcataact aagttctagg tcaatcacta catcaataat
     5881 agcttataca taagtaagaa tcaatataat attaatatct tattttccaa atttattact
     5941 atttaaaaaa gactgaagat gaaaagagat agaaacgatt tatttgcttg tatttaccga
     6001 agtgtaagga gtttgattga caataattgt taaaatagaa gttggttgta attagccaac
     6061 cataacccta cctgtcatcc atggatgtat taaacaatga taaccgaatt ctcctgtaga
     6121 atcaaacgta ttatcccatg tcatgccagg aggtattgga cctgagtcaa aaagtggtgc
     6181 accagttcct tcagcagtta ccgtgtgttg tgtagaatct tcatttgtcc atgaaaccat
     6241 acttcctggc ataacattaa gtgcacgtgg agtaaatggc tcaaagttgc cttcatcggc
     6301 tgctccaagt gtaatcacta ctgaacttgg ttcttgttga gaaaaggcaa gtgttggatt
     6361 ggataaaatt ccaaaagtta gcagtatact acctattatg cttaaattca agatatttac
     6421 tatatacatt taatcttcta tccgggatat atatataaaa attttagttg agtttgtctt
     6481 agtaaatatt atatagaaaa tatttaggtg aattaataat tatttttaat gattatatga
     6541 gagacaagta tttaaccagt atttgtagtg gcttagtctg aacttataat taactaaata
     6601 gttataatca aaatttaaaa attcaataac acaaggtgta cacgcaaatg gtcgatttta
     6661 tatcttccag atgcaatgat tatactaaat tcaactaatc acatatttgt gtaataacaa
     6721 attaaattag tgctgaacta tacttgtgtt tgtca
//
LOCUS       DASVEV010000204         5484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_87473, whole genome shotgun sequence.
ACCESSION   DASVEV010000204 DASVEV010000000
VERSION     DASVEV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87473"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1108..1962)
                     /locus_tag="VF222_09205"
     CDS             complement(1108..1962)
                     /locus_tag="VF222_09205"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7142640.1"
                     /translation="MKQVALITGCSSGIGYETSILLAKNGFRTYATMRNTDKGDKLKE
                     IAEKENLDLKIIKLDVTDDYSIKNAINEIVQETNRIDVLVNNAGNNIAGAVEDLSIEE
                     FKEQFETNFFGLIRVTKVVLPIMRKQNNGIIVNLSSIAGKMAIPLNSAYVSSKFAIEG
                     FSESIRYELEDFGIKVILIEPGVIKSNFYENIKMSKNSLLDEKSAYQPITQKIFEAFL
                     PMLEYAFPTKIVADIILEAVNSDNPNIRYAVGDDAKSIIEARKKLSDKEFENWVKEGF
                     FEKKGFIR"
     gene            2917..3804
                     /locus_tag="VF222_09210"
     CDS             2917..3804
                     /locus_tag="VF222_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX7142641.1"
                     /translation="MVYIRIKQVKNIGYAYLVESKWNKEKHTSQQIIKRYLGPVSHIN
                     INKIPEEYRNESTVLKFLASRNIDFSKSRELNDSIKANLLEKLKSYEINDLIKIYHDY
                     TTSVYSSLDFYEKILIPVLYKIGDLWANEEIDIATEHVCSNAVNTLIDLINQKNTKKN
                     SKSYGDTKSIVLCTPEGELHSIGCKVIESLLLEKGYEVYNITSPLPTNSIESYLDKTN
                     PGVVLISVTLEENINSAIRLVREIRKSFQIPIIIGGNAIKFASKSQIQLLEKYDKVYI
                     DLENLADIITNVKVILDKK"
     gene            4074..4604
                     /gene="hsp20"
                     /locus_tag="VF222_09215"
     CDS             4074..4604
                     /gene="hsp20"
                     /locus_tag="VF222_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HEX7142642.1"
                     /translation="MWKEPFDFRKIFDEFDREFSTAEEMLSRIFRTVREGDEQPLVRN
                     SPYYYGYQINVGPDGKPHVREFGNVRPTRKGLVEQSEIRQPLVDTNFNEKENTYVITA
                     EMPGITKEDIKVSVSQQTVTIRAERAQKKYLTEIPFDVELDDTSAKATYTNGIVEVKI
                     KTKERPKLKSKEIKVE"
     gene            4667..>5484
                     /locus_tag="VF222_09220"
     CDS             4667..>5484
                     /locus_tag="VF222_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7142643.1"
                     /translation="MYNYPNSDDDNNKFNLKVAETFSKFVGRGIALIDPRIIEQYDLR
                     TGDVLELTHESTSKKSYVLLWSSNPNDYGKGLIRIDGYTRENISVGIDDQINVQKVNV
                     EKAAEITMSPIVDLNIVGLEDYMSEKLDGRVVAKDDVILFNIMGKRIGFLIVATKPQN
                     RALLINSHTKFKIGSTSDIAVQDKKIDRITYEDIGGIKNEVQKVREMIELPLRHPELF
                     DKIGIEAPKGVLLHGPPGTGKTLLAKAVANETNANFYSIGGPEIMSKFYGESEE"
BASE COUNT         2032 a          743 c          755 g         1954 t
ORIGIN      
        1 tctcctattt ctttctcaaa atcttgaatt gtctcaggac ctgggtcgga agtatttgca
       61 gtctgtgcaa cagcgaaatt aattccattt gtatgtccta caaagaggaa agaaaacaca
      121 gcactgaata gtaatactgc tactactact atattagttg tttttatcat taccaatact
      181 atagaagatt attataaaaa ttttactaac ttctatctct taattctatc gttaaatcaa
      241 ttctatcact tacaaatatt tcaaaaaaca atacttatcg tttttattaa agatgatgct
      301 aaaaattaat tgagtcatat aatataaatg agttttcaat agtgcagcgt aatctaatgt
      361 atgttttaga gtaataaaaa aacaatctat tccttttata atcatatagc gttaataata
      421 ttcgaggaat agagatgagt taatatctta attttatatg gctttatttt aaatcaataa
      481 taataataat tataataatt ataatcaaaa ttttaaatga catttttact ttttttgttt
      541 gaacttaatt acataatttt cataagatta aaatttgctt ttctagtata caattttata
      601 agttgaaatt taatttactt aaggctatta aaaatacaca cagacatcca attaatatta
      661 tattacattg tattggatta cctctctata ttattggaat ttattttatc atatttttct
      721 ttatagatca aaataaatct ctattgtacg gtattatttt gtggatgagc gcaattagtt
      781 tatttatctt tggtcatatg atagaaggca attttagagc aatgacatta gttgttatct
      841 ataagtattt gagatccttt ctgcgaaatt agaaccataa tttaatgcaa taaccgtacc
      901 ttgtatttga aataaatagt taaaatctaa ttacatattt tactttgtta ttaataaggc
      961 aataatctat tctgatatga taaatgactg aactataaaa cgaatataac aatataacaa
     1021 tatatgtatc aattgaattt atttattttt tattcattat agttatcaat ttcaaaaata
     1081 ttatagctat tgcttattat ttttttatta tcttataaat cctttttttt caaaaaaacc
     1141 ttctttaacc caattttcaa attctttatc cgatagtttt tttctagctt caatgatcga
     1201 tttagcatca tctcctactg catatcttat atttggatta tcagagttta ctgcctctaa
     1261 aattatatca gctactattt ttgtaggaaa agcatattcc aacattggca aaaatgcttc
     1321 aaatattttc tgggtaattg gttgatatgc agatttctca tccagtagtg aatttttaga
     1381 catttttata ttttcataaa aatttgattt tattacacct ggttctatta aaattacctt
     1441 aataccaaaa tcttctagtt catacctaat agattcagaa aatccttcta tcgcaaattt
     1501 actacttaca tacgcggaat tcaacggaat agccattttc ccagctattg aacttaaatt
     1561 aacaattata ccattatttt gttttctcat tatgggcaaa actacttttg ttactctaat
     1621 taacccaaaa aaattagttt caaactgttc cttgaattct tcaattgata gatcttcaac
     1681 tgctcctgca atattgttac ccgcattgtt aactaaaaca tcaattctat tggtttcctg
     1741 aactatttca tttatcgcat tttttatcga atagtcatct gtgacgtcca gtttaatgat
     1801 tttaagatca agattttctt tttcagctat ttcttttaat ttatcgcctt tgtcggtatt
     1861 tctcattgtg gcataggttc taaagccatt tttagctaat aaaattgaag tttcatagcc
     1921 tatacctgat gagcagccag taattaatgc aacttgtttc aattgttttc cacttatata
     1981 tttgaatttc atacgcaatc gataaacttt tcgttaattt tattatcctt actgtctttg
     2041 atataataga aatttatttc agttcaataa ttacaatgta ttgatttgat tcattttgct
     2101 ttatttattt ctaaataaag ttatctagac acaataaacc ctctttaacc tggaacaaga
     2161 atagttattt tcctttttat tatgattttt ttatttgtat attttaatat ttttaatgta
     2221 attaagtttt tgctctatat ccttcaaatt tatttctact atttctttct tttgatcaaa
     2281 aaattataat atactataag tgctctccca ccattactac catcctgatt caatttttgg
     2341 ttattttaca tgaaattctt ctattcaatt ctatttcagg ctctcaagaa tatcttgatc
     2401 aagtttattg attttactat agtagttata cttcaaaaaa gtataaccaa taataataac
     2461 aatactgcca ataattaaga gagaagatat taaagtattt tcaaaatttg aaagactagc
     2521 gaacattatg atagatatta aagctaatat acctgttaca ataattttta attttagaaa
     2581 ttttatatat tgtctcatat taactagagc cttcaactat tatttataca agtggtcaaa
     2641 tcatttcata gtgctaactt tatatgtcat gaaattttaa gagagaaatt taaggccgtg
     2701 ttaaaaagat aaaaggcaaa atttgaaatt ttattattat taattatgtt actagagcat
     2761 tccagctagt tcaagtaaag aagtaaatag tttcggaaaa ttgtaattta ttagcatata
     2821 aattgataat tttataaact tcttacgaca caattagcac tatttataac ctttaggcac
     2881 taaacattaa taaaagacgt gatagagcag tatattatgg tatacattag aataaaacaa
     2941 gtaaaaaaca ttggttatgc ttatcttgtt gaaagtaaat ggaataaaga aaaacatact
     3001 tctcaacaaa ttataaaacg gtatttaggt ccagtatctc atataaatat caataaaatc
     3061 ccagaagaat atagaaatga atcaaccgtt ttaaaatttt tagcttcacg taatattgat
     3121 ttttcaaaat ctagagaact aaatgattca ataaaagcga acttgctcga aaagttgaaa
     3181 agctacgaga taaatgacct gataaaaatt tatcatgatt atacaacatc agtctattca
     3241 tcattagatt tctatgagaa aatactgata cctgtattat acaagatagg tgatttgtgg
     3301 gcaaatgaag aaatagatat tgcaacagag cacgtctgca gtaatgcagt aaatactcta
     3361 attgatttaa tcaatcaaaa aaatacaaag aaaaattcta aaagttatgg agatactaaa
     3421 tcaatagttt tatgcactcc agaaggagag cttcatagta taggttgtaa agtaattgaa
     3481 tcgttattgc ttgaaaaagg atatgaagtt tataatatta cttcacctct tccaaccaat
     3541 tctattgaat cttatttgga taaaaccaat ccaggtgtag tactcatatc tgtcacttta
     3601 gaagaaaata tcaattctgc gattagacta gtacgtgaaa ttcgtaaatc tttccagata
     3661 cctataatta ttggtggcaa tgctataaaa tttgcatcaa agtctcaaat tcaattatta
     3721 gaaaaatatg ataaagtcta tatagacttg gagaaccttg ctgatataat aactaacgtc
     3781 aaggtaatat tagataaaaa ataataaatt tatagcactt tatatcactt ttaacacaat
     3841 aattctaatc aaattgtttg atgataataa attggagttc atcaatctcg aatattcatt
     3901 atgtaaaata cttggcattt attatgtatc acttttacca actacgtctg ctcgaacctt
     3961 aagcattaaa tctatttaat agagtttaga tggtgtaatt tggtaatttt cacaccatac
     4021 ttgtaagata aatttatata gtgtaataga cattacataa tgtaagaaat aaaatgtgga
     4081 aagaaccatt tgattttaga aaaatatttg atgaatttga tagagaattt tcaacagcag
     4141 aggaaatgct tagcagaatt tttagaacag tcagggaagg agatgaacaa ccattggtaa
     4201 ggaattctcc ctattactat ggatatcaga tcaatgtagg acctgatggt aaacctcatg
     4261 ttagagagtt tggaaatgtt agacccacaa gaaaaggatt agtagagcaa agcgaaatta
     4321 ggcaaccatt ggttgacaca aatttcaatg aaaaagagaa tacatatgta attacagctg
     4381 agatgccagg aataactaag gaagatatca aagtaagtgt ttcacaacaa actgttacaa
     4441 taagagcaga acgggcacag aaaaaatatc ttactgaaat tccatttgat gtagaattgg
     4501 atgatacttc tgctaaagct acatatacaa atggaatagt agaagtaaaa ataaaaacaa
     4561 aggaacgacc taagctcaaa tctaaagaga taaaggttga ataaaatcct aattttttta
     4621 ttttttttaa aatttaatag tataatacat gaggtgattt aataaaatgt ataattatcc
     4681 gaattctgat gatgataata ataaatttaa cttaaaagtt gcagaaacct tttctaaatt
     4741 tgtaggacga ggaattgcac ttattgatcc tagaattatt gagcaatatg atcttagaac
     4801 aggtgatgtt ttagagctga ctcacgagag tacatctaaa aaaagttatg ttttactttg
     4861 gtcaagtaac ccaaatgatt acggtaaggg attgattaga atagatggct acacaagaga
     4921 aaatatttct gtgggaatag atgatcaaat aaatgtacaa aaggttaatg ttgaaaaggc
     4981 agcggaaatc acaatgtctc caattgtaga tcttaatata gtcggattag aagactacat
     5041 gtcagagaaa ttagatggaa gagtagttgc caaagatgat gtaatacttt ttaatataat
     5101 ggggaaacgt ataggtttcc taatagtagc tacaaaacca caaaatcgag cactattaat
     5161 aaattcacat accaaattca aaattggatc tacatctgat attgctgttc aagacaaaaa
     5221 gattgataga attacatatg aggatatagg gggaatcaaa aatgaagtac aaaaagtaag
     5281 agaaatgatt gaacttccat taaggcatcc cgagcttttt gataaaattg gaatagaggc
     5341 accaaagggt gtactattac atggacctcc aggtacgggt aagaccttgc ttgcaaaagc
     5401 agttgctaat gagacaaacg ctaatttcta ttctatcggc ggaccagaaa ttatgagcaa
     5461 attttatgga gaaagcgaag aaaa
//
LOCUS       DASVEV010000205        10500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1447696, whole genome shotgun sequence.
ACCESSION   DASVEV010000205 DASVEV010000000
VERSION     DASVEV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447696"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF222_09225"
     CDS             complement(<1..491)
                     /locus_tag="VF222_09225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017677.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HEX7142644.1"
                     /translation="MVSNFVDIWPIINDILRREGIARQHLNSYNEFMESGLQSIIDEV
                     NEVEIETAEFPYKLKLGKIKLQRPRIMELDGSITHVAPVEARLRNLTYASPIMLECSI
                     VEDGRIIESRFIHVGDMPVMVKSNVCILHNLPESKLIELGEDPRDPGGYFVINGSERV
                     IVGL"
     gene            complement(507..758)
                     /locus_tag="VF222_09230"
     CDS             complement(507..758)
                     /locus_tag="VF222_09230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HEX7142645.1"
                     /translation="MSAEKKIVKVNNHIYQPKHEILSKDESEAILKKFHVRTNQLPYI
                     LASDKGLEELDIRPGDIIKITRKSPTAGESVYYRYVVQG"
     gene            complement(951..1054)
                     /locus_tag="VF222_09235"
     tRNA            complement(join(951..985,1016..1054))
                     /locus_tag="VF222_09235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1017..1019),aa:His,seq:gtg)
     gene            complement(1125..1940)
                     /locus_tag="VF222_09240"
     CDS             complement(1125..1940)
                     /locus_tag="VF222_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX7142646.1"
                     /translation="MKGLNTADLMKIGLDLAGWKKIPYDSAIHLTGKNIKKVFVCIDV
                     STSDISLAKKIGCDCVLTHHPIGKSQLTFHKVFDRHVEYMMEKGIKKKLAFSLVNDLK
                     NKASLKSHSLIYNDVISTAQILEMPLLNIHQPLDEYMRNVILFKLSRSKLRSVSDLVE
                     AIEKIPEFRKALTRVIVCNGKSNNELGEWVLVIAAGSNGGYDIARTYYENNISTVIYL
                     HIDYNELLKVKKDKRCKNLVILGHLAGDSIGMNKICKELEKREIVVVKRGLIG"
     gene            2108..2587
                     /locus_tag="VF222_09245"
     CDS             2108..2587
                     /locus_tag="VF222_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142647.1"
                     /translation="MFKKFINFYLRYQNINLKISFILISLQLLHLYWLTTDVVIYRLT
                     GIDYFVGLSDFILLFIIIDYIEVPALVSGTVYYLFALIYDKKEKEKRLKNFILLILLA
                     IQSIHIFWITDDIVYSTFVGSDLISMPEYFVWIAILIDYLELPVIYDLFKRTIKKEK"
     gene            complement(2724..3788)
                     /gene="twy1"
                     /locus_tag="VF222_09250"
     CDS             complement(2724..3788)
                     /gene="twy1"
                     /locus_tag="VF222_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HEX7142648.1"
                     /translation="MSCSDEYVSHDKQNNLINITPRLQAKLRKAKYGVYNHSAVELCH
                     WTKKSFNNEGNCYKHKFYGISTHRCMEMTPTAMNCENRCVYCWRPTEFYDTLQMPEHL
                     VDEPDAIIHHLLKERERLIMGFYGSSKSNKKKLDESLFPAHYAISLSGEPTMYPKLPD
                     LIRYLKSLKNTKSVFLVTNGQEPEMIARLIKEDSLPTQLYLSTNASNKKMFYQINRPK
                     YKDAWNRWLNTLNILSEINTRTVLRMTLIRNYNSDLNHIPEFVNLINKANPHFIEVKS
                     YMHIGMSTNRLEHNNMLEVEKVREFTSNLQKTLQGYNIMDESEISRIVVLQNQKRYTD
                     RWIKEYFEPNILQEQYISKP"
     gene            3914..4453
                     /locus_tag="VF222_09255"
     CDS             3914..4453
                     /locus_tag="VF222_09255"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142649.1"
                     /translation="MSKTLAIDLDSVLADVVVTWVNEYNILKDSNITKEDIVAWDIHT
                     LLPITETESNSLFSDVWVYRWKDIPPTQNGIGKIIERLKTENYRISILTRRERKTIPY
                     VYYWLETNSIDCDDLIYIFDSNPKSIYPFDILIDDAPQNIVDIKFPKKAILFDQPWNR
                     NFDWPIRINKLSDLSHSLP"
     gene            complement(4479..4817)
                     /locus_tag="VF222_09260"
     CDS             complement(4479..4817)
                     /locus_tag="VF222_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142650.1"
                     /translation="MNNKRIIMDVETSNLLQQPPENSKIILKNVVYDNKHFIKKLELL
                     IYITNNHKLLGDYSILVSIIETNNGTVIMKYDEGYRGKDAFDNAIEMLTQYSGLSSLI
                     NRSLIELDNT"
     gene            complement(4810..6189)
                     /locus_tag="VF222_09265"
     CDS             complement(4810..6189)
                     /locus_tag="VF222_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142651.1"
                     /translation="MAGIVAIYNFDTKRNSSGEILFYLVSSMRMVQHRGKAYWKMMIG
                     ERVLESKGSIPSNDVLARLIKKKKIIGSNAIGYLSKRAPIFPSMDSIKIAFDGFLVDT
                     EKLNLHPYIGSAKDADPLFQIYSIFIRLIDEKKDNSRAAEFLDRHLRGNLILKVNDSI
                     FVFRNSTGFKPLIMALAPNKDLVIFSSENSLKISFANFRYTDVKMGQLLKLTDQNHIQ
                     ELTILESNKILMDPFEFIRESHVTTTFDKKSIYTIRRNIGKVQANYLSKYVDIDTVYA
                     EPDYTRPMALGIGIELQKSILNFEISEGVIKDRYDDSDHMIDFSEEESKNKLLTTGKS
                     LKFILENITKDKKVASIQGTIQTGSTAKETIFYLNKSGVKKIDVIVSYVPTIDGRQVG
                     LYTSNRDFIANKYMGRVTSIEELNKHIANDLGCEKTFYNSPYLLAKGIGVSENNLWFP
                     EWIRFLDYE"
     gene            6343..7545
                     /locus_tag="VF222_09270"
     CDS             6343..7545
                     /locus_tag="VF222_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7142652.1"
                     /translation="MIIGVIGKANVGKSTFFNSATDQSVPSANYPFTTIQPNIGISFV
                     REKCVCREFGVQDNPLHSICIDGNRFVPIKLIDIAGLVPGAHLGKGLGNKFLDDARQA
                     DALIHVVDASGSTDPEGRVVEKSHGDPSFDIKFVEEEFDQWVLSIVNRDWNKTSRDMA
                     NQKLKIEQILTKRLSGLSVTVDKISNALSNLGLVNKNPDEWTQAELLEFVRLIRKNSK
                     PLLILANKADISSSKENINKIKQQNNIVIPCASEGELVLRKGAKKGLWYYLPGDSSFV
                     LKDKDSLNEQQKKALSIIQSVLDKYGSTGVQEAINSVCFKLLNLIIVYPVEDEIKLSD
                     KKGHVLPNALLLRQGSTAKDLAASIHEELAKGFMFAIDVRNKQRIGADYELKHNDVIK
                     IVSTTSRG"
     gene            7548..8582
                     /gene="kae1"
                     /locus_tag="VF222_09275"
     CDS             7548..8582
                     /gene="kae1"
                     /locus_tag="VF222_09275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HEX7142653.1"
                     /translation="MKIKSKDDHLIFCLGIESTAHTFGCSVIQFDLINKRGKIISESR
                     SVYKAPDGAGIHPREASRHHLDKSPTILRQVLIQSNLKPKEIDLVSYSAGPGLGPCLR
                     VGAVIARVLSSFYNIPLIPVNHALGHIELGMMLTECRDPLVLLVSGGHTMILAFSNKK
                     WRVFGETQDITIGQLLDQFGRYMGLASPCGNKIEKYANQSSKKYYQLPYIVKGNDVSF
                     SGILTATKKLLDNGYDKFNICFSLQETAFAMLSEVVERALSFTDKKEVLIVGGVSANN
                     RLSNMLKIATKRQKATLYTCPIHYSGDNGAQIAWTAICDNISSGKNIKVQNALIKQSW
                     RLDKVEVNWR"
     gene            complement(8613..8939)
                     /locus_tag="VF222_09280"
     CDS             complement(8613..8939)
                     /locus_tag="VF222_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142654.1"
                     /translation="MSSVQPRKGEKLVLQEDCYEDHLKNGLLTLTNQRLLFEKTEGTL
                     ATLSKRTGELLLNIPLVEIKASKGEGFLLKKVVVITIQNDIYKFGVFSNSKWAKEIEK
                     SKSNIQ"
     gene            8972..9586
                     /locus_tag="VF222_09285"
     CDS             8972..9586
                     /locus_tag="VF222_09285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF011462.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HEX7142655.1"
                     /translation="MHYKIIKRGAEADVFLIPWYENMAISKLRNPKKYRNKILDNLIR
                     GRRTIYEAKMLKNVKTTGIKTPFIYFLDPKRAEIIMEYIDGENVKDILSKEIGYEMGK
                     YVSVLHNKNIIHGDLTTSNFIKRKHDLYLIDFGLSFYSERIEDMGTDIRIIKEILGSA
                     HIHVFEETYQSFLEGYSACTNYNFSRILKVVKDIEARGRYARVE"
     gene            9663..10256
                     /gene="rdgB"
                     /locus_tag="VF222_09290"
     CDS             9663..10256
                     /gene="rdgB"
                     /locus_tag="VF222_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7142656.1"
                     /translation="MNEIYFVSHNIDKYSEIKSILNNRITDLHLKFCKRNITEIQDQK
                     IEKIAIEKAKLAYNIVKKPIIIEDDGLFINSLNGFPGQYSSYVLKSIGNRGIIRLLKG
                     YKDRSAFFKSIFVYSNGISVKVFYGKINGKISSTIKDDGWGYDPIFIPLNNNNDNNSD
                     TTFAKLSKTNKKNELSHRRIALDKFVKWFNQNNKLNI"
BASE COUNT         3662 a         1572 c         1543 g         3723 t
ORIGIN      
        1 agtcccacta tgactctttc ggatccattt atcacaaaat atccaccagg atctcgaggg
       61 tcttctccta attctattaa tttagattct ggaagattat gaaggataca tacattagat
      121 tttaccatga caggcatatc acctacatga atgaatctag attctataat acgaccatct
      181 tctacaatac tgcattcaag cataattggg gatgcatatg taagatttcg taacctagcc
      241 tctacaggag cgacatgagt aatggatccg tctagctcca taattcgggg acgttgaagt
      301 tttatttttc ctaatttcaa cttataggga aattcagctg tttctatttc gacttcgttt
      361 acttcatcta ttatactttg taatcccgat tccataaact cattgtagga attcaaatgc
      421 tgtcgtgcaa taccttctct tcttaaaata tcattaataa taggccatat atcgacaaaa
      481 ttacttacca aataacttgt atctccttat ccttgtacta catatctgta atatacactt
      541 tcacccgcag ttggactttt ccttgtaatt ttaattatgt ctcctggtcg aatatctaat
      601 tcttccaatc ctttatcgct tgccagaatg tatggtagtt gattagttct tacgtggaat
      661 tttttaagga ttgcctcaga ttcatctttt gaaagaattt cgtgtttagg ttgatagata
      721 tgattattta ctttgacaat ttttttttct gctgacaatt aataaccctc ctctaaattt
      781 acccctagat aaataaatct tgataaaata gcttctcgca atacgaaatc agtaactttc
      841 ttggagtagg aggtatatgt agtttcttac aacatttcat tcgatacata ttaaattgac
      901 taaacctgtt tatgaaatac tattaataat gacattgttt tgattattta ggccttcgga
      961 gggatttgaa ccctcctcaa gcggtttcta tctttcataa tttataataa aaagaccaca
     1021 gccgcctata ctaaccaggc tatactacga aggccactaa ggttttataa aaatctgtat
     1081 tatttattct ttagagaaat aaaaatcaca atcaaaattc tgtattatcc aattaaacca
     1141 cgtttaacca ctactatttc ccttttttct aattctttac aaattttatt cattcctatt
     1201 gaatctcctg ccaaatgtcc aagaataaca agattcttac acctcttgtc ttttttaact
     1261 tttaaaagtt cattataatc aatatgaaga tatattacgg tagaaatatt attttcataa
     1321 taagtccgtg caatatcata tccaccattc gatccagctg ctataacaag tacccactca
     1381 cctaattcat tgtttgactt tccattgcaa actatgactc ttgttaaagc ttttctaaat
     1441 tctggtatct tttctattgc ttctactaaa tccgagacgg atctcaattt ggatcgagaa
     1501 agtttgaata aaataacgtt tctcatatat tcatcaagtg gctggtgtat attcaataag
     1561 ggcatttcga gaatttgtgc ggtgctaatc acatcattgt aaattaaaga atgagatttt
     1621 aaagaagcct tattttttaa atcattaacc aatgaaaatg ctaatttttt ttttatcccc
     1681 ttctccatca tatattccac atgtctatca aaaactttgt gaaatgtaag ctgtgacttt
     1741 cctattggat gatgtgttaa cacacaatca caacctattt ttttagcgag agagatatca
     1801 gaggtactca catctatgca aacaaaaacc tttttgatat ttttaccagt tagatgaatc
     1861 gcactatcat atggtatttt tttccatccc gctaaatcca gtccaatttt catcaaatca
     1921 gctgtattca atcctttcaa tgtattataa aattattata attgtggaat atttactttt
     1981 aaacgaatct cttcatccat ttctaaattt tatctgatta atgatttgtt aagaatatca
     2041 aatatagact attatattga taggtaaaaa aataataata ataataacta tgaaaaattg
     2101 atttattgtg tttaaaaaat ttattaattt ttatcttcgt tatcagaata ttaatttaaa
     2161 aatctcattc atattaatct cattacaatt attacatctt tattggctta caacagatgt
     2221 tgtaatatat cgtcttaccg gaatagatta ttttgtaggg ctatctgatt ttattttatt
     2281 atttattata attgactata tagaagttcc tgctttagtt tcaggtacag tatattactt
     2341 atttgcttta atttatgata aaaaagaaaa agaaaaaagg cttaaaaatt tcatcttatt
     2401 gattctattg gcaatccaat ccattcatat cttttggatt accgatgaca ttgtttattc
     2461 aacttttgtt ggatctgatc taatttctat gccagaatat tttgtttgga ttgcaattct
     2521 aatagattat ttagaactac ctgtaattta tgatcttttt aaacgaacca tcaaaaaaga
     2581 aaaataaaaa aagaaaaatc aagtcgaggt attacatttt atagagtata tacccttgag
     2641 aacttagata tttaatacta tgaatgtaat gtgaccttag gaattgaact aaccacttag
     2701 tatagataat tataaagagc tgactacggt ttacttatat attgctcttg taggatatta
     2761 ggttcaaaat attcttttat ccatctgtca gtatatctct tttgattttg caaaactact
     2821 attctagaaa tttcactttc atccatgata ttataacctt gtaaggtttt ttgcaggtta
     2881 ctagtaaatt ctcttacctt ctcaacctca agcatattat tgtgctctaa cctattggta
     2941 gacattccta tatgcatata tgatttaact tcaataaaat gtggattcgc tttattaatt
     3001 aaatttacaa attcaggtat atgatttaaa tcagaattat agttacgtat gagagtcatt
     3061 cttaaaaccg ttcgcgtatt aatttcagaa agaatattca atgtgttaag ccatcgattc
     3121 caggcatctt tatatttagg tctatttatt tgataaaaca tttttttatt tgatgcattt
     3181 gtagacaagt ataactgggt tgggagagag tcttccttta ttagacgagc aatcatttct
     3241 ggctcctggc catttgttac caaaaatacg gactttgtat tctttaatga tttaagatat
     3301 ctaattaaat cgggaagttt tggatacatt gttggttctc cagataaaga aatagcataa
     3361 tgagccggaa ataatgattc atctaatttt ttcttattcg atttagaact tccataaaat
     3421 cccataatta gcctctctct ttcttttaat agatggtgta taattgcatc tggttcgtca
     3481 actaaatgct caggcatttg taatgtatca tagaattctg ttggacgcca acaatataca
     3541 cacctatttt cacaattcat tgcagtagga gtcatttcca tacatctatg agtagatata
     3601 ccgtagaact tgtgtttata gcaattacct tcattattaa aggatttttt ggtccagtgg
     3661 cacaattcta ccgcagaatg gttatataca ccatactttg ccttacgaag tttggcttgt
     3721 aacctaggag taatatttat caaattattc tgtttatcat ggctaacata ttcgtccgaa
     3781 caactcataa actgaaataa atattaaact tatttatatt tttgattaat atatactaac
     3841 tattataatt actaaatgac agagaaaata caattacttt tacatatagg catataacac
     3901 taatatcatt cgattgagta aaactctagc tattgattta gactcggttt tggcagatgt
     3961 agtagttacg tgggttaacg aatataatat actaaaagat tctaatataa caaaagagga
     4021 tatagttgct tgggatattc atactctttt accaataaca gaaaccgaaa gtaactcatt
     4081 attttctgat gtatgggtat atagatggaa agatatacct cctactcaaa acgggattgg
     4141 aaaaataata gaaagattaa aaactgaaaa ttatagaata tctattttaa caagaaggga
     4201 aaggaagaca attccttatg tatattattg gttagaaact aatagtatag actgtgatga
     4261 tcttatctac atttttgatt ctaatccaaa aagtatttat ccatttgata ttttaattga
     4321 tgatgcccct caaaatatag ttgatataaa gtttccaaag aaggcgattt tatttgatca
     4381 accctggaat agaaattttg attggccaat tagaataaat aagttatcag atttatcaca
     4441 tagtctaccc tgaattttta cacgtacaaa ataaaaaatc aagtgttatc taattcgatt
     4501 aatgatctat tgattaatga tgataatcca ctatattgtg ttaacatttc tattgcatta
     4561 tcaaaagcat ctttgccacg atatccttca tcatatttca ttataaccgt tccattattt
     4621 gtttcgatga tactgactag aatagaatag tctcccaata atttatggtt atttgtaata
     4681 tatattaata attctagttt tttaataaaa tgtttattat catacacaac attttttaat
     4741 attattttcg aattttccgg aggttgttgc aataaattac ttgtttcaac gtccatgatg
     4801 attcttttat tattcataat caaggaacct gatccattct ggaaaccata aattattctc
     4861 tgaaacgcct atgcctttgg ccaataaata tggagaatta taaaacgttt tttcgcatcc
     4921 aaggtcatta gcaatatgtt tattaagttc ctctattgaa gtaactcttc ccatatattt
     4981 attagcaata aaatctctat tagaagtata taatccaact tgtcgtccat caatagtagg
     5041 aacataacta actattacat ctattttttt tactccgctt ttattcaaat aaaaaattgt
     5101 ttcttttgca gtgcttcctg tctgaatagt tccttgaatg gaagccacct ttttatcttt
     5161 agttatattt tctaaaataa atttcagcga ctttccggtg gttagcaatt tatttttact
     5221 ttcctcctcc gaaaaatcaa tcatatgatc tgagtcgtca tatctatctt tgattactcc
     5281 ttcagagatt tcaaaattta aaattgactt ttgtaattct attcctatgc caagagccat
     5341 aggtctagta taatctggtt ctgcatatac agtatcgata tcaacatatt ttgatagata
     5401 atttgcttga acttttccta tgtttcttct tattgtatat atactttttt tgtcaaaagt
     5461 ggttgtcaca tgagattctc taataaattc aaaagggtcc attaaaattt tattactttc
     5521 cagaattgtt aattcttgta tatgattttg atccgtaagt ttaagcaact gtcccatttt
     5581 tacatcagta tatcgaaagt tagcaaatga aattttcaaa gaattttctg aagaaaaaat
     5641 tactaaatcc ttgtttggtg ccaatgccat tatcaatggt ttaaatccag tactattccg
     5701 aaaaacaaaa attgaatcat ttacttttag gataagattt cctcgcagat gtctatctaa
     5761 aaattctgcc gcacggctat tatctttttt ctcatctatt aatctgatga atattgaata
     5821 aatctgaaac aatgggtcag catcttttgc agatcctata tatggatgaa gatttaactt
     5881 ttcagtatca accagaaaac catcaaacgc aatcttgata gaatccatgc tgggaaatat
     5941 cggagcacgt ttggaaagat aaccaatagc atttgaacca attattttct ttttttttat
     6001 taatcttgct aacacatcat ttgacggaat ggaaccttta ctttctaaaa ctctttctcc
     6061 aatcatcatt ttccaatatg cctttcctct gtgttgaacc atccgcattg agcttacgag
     6121 gtaaaaaaga atctctcccg aagagtttct tttagtatca aaattgtaaa ttgctactat
     6181 cccagccaag atccttgaac tttcataagt agttttattg aatatataaa aattttagaa
     6241 aatgggaata taataattaa aattccttag gagacgagag tatcttaaat taaaatagaa
     6301 aaataaaaaa tactgataat actagtataa ttgattctta ttatgattat aggggttatc
     6361 ggaaaagcta atgttgggaa atctactttt tttaattctg ctacggatca atctgtgcct
     6421 tcagcaaact atccttttac aactatccaa ccaaatatag gaataagttt tgtcagggaa
     6481 aaatgtgtat gtagggaatt tggagtacaa gataatccac tacattcaat atgtatagat
     6541 ggaaatagat tcgtacctat aaaattaata gatattgcag gacttgttcc tggcgctcat
     6601 ttaggaaagg gtttaggaaa taaatttcta gacgatgcaa gacaagctga tgcattaatc
     6661 catgtagtag acgcttcagg gtcaactgac ccagagggta gagttgtaga aaagtctcat
     6721 ggagatcctt cctttgatat taagtttgtc gaggaagaat ttgatcaatg ggttctttca
     6781 attgtcaata gagattggaa taaaacatca agggatatgg caaatcaaaa attaaaaatt
     6841 gaacaaatac taacaaaaag actatctgga ctatctgtaa cagttgataa aatttcaaat
     6901 gcgttgtcta atctaggatt ggtcaataaa aatcctgatg aatggacaca agcagaatta
     6961 ttagaatttg taagattgat aagaaaaaat tcaaagcctt tactaatact agccaacaaa
     7021 gctgatattt cttcatccaa agagaatatt aataaaataa aacagcagaa caatatagta
     7081 attccttgtg catcagaagg tgaattagtt cttaggaaag gagcaaaaaa aggtttatgg
     7141 tattatcttc caggagactc aagttttgtg ttaaaagata aagattcatt aaatgaacaa
     7201 caaaaaaaag cattatcaat aatacaatca gtactagaca aatatggatc tactggagtt
     7261 caggaagcta taaactcagt atgtttcaaa ttactaaatc taatcatagt atatcctgtt
     7321 gaagatgaaa taaaattatc tgataaaaaa ggccacgtcc ttcctaatgc attgttatta
     7381 cgacaaggat caacagcaaa agatttggct gcaagtattc atgaagagtt agctaaagga
     7441 tttatgttcg ctatagatgt aagaaataaa caaaggattg gagcagatta tgaattgaaa
     7501 cataatgatg tgataaaaat agtatctact actagtagag gataattatg aaaattaaaa
     7561 gtaaagatga tcatcttatt ttttgtcttg gaatagaaag tactgcgcat acttttggat
     7621 gttctgtaat tcaatttgat cttattaata aaagaggtaa aataatatca gagtctagaa
     7681 gtgtatataa agctcctgat ggagcaggta tacatcctcg ggaagcttct agacatcatt
     7741 tggacaagtc tcctactatt ctaagacaag tgttaataca atctaattta aagcctaaag
     7801 aaatcgactt agtctcttat tccgctgggc cgggtttggg accttgcctt agagttggag
     7861 cggttattgc tagagtgctt tcatcatttt ataatatacc attaatacca gtcaatcatg
     7921 ctttaggaca tattgaatta ggtatgatgc ttacagaatg tcgagatcct cttgttttat
     7981 tagtctctgg aggtcatacc atgatattag cattttcaaa caagaaatgg agagtctttg
     8041 gcgaaacaca agatataaca atcggtcaat tattagatca atttggaaga tatatgggcc
     8101 ttgcttctcc ttgtggaaat aaaattgaaa aatatgcgaa tcaatcttca aaaaaatatt
     8161 atcaattgcc ctatattgta aaaggcaatg atgtttcatt ttctggaatc ttaactgcta
     8221 caaaaaaatt gctagataat gggtatgata aatttaatat ctgtttttcc cttcaagaaa
     8281 ctgcatttgc aatgttaagc gaagttgttg agagagcatt atcttttaca gataaaaagg
     8341 aagttttgat tgtaggagga gtttctgcaa ataaccgtct ttctaatatg ttaaaaatag
     8401 caacaaaaag gcaaaaagcg acattgtata cttgtcctat acactattca ggtgataatg
     8461 gtgctcaaat agcatggaca gcaatctgtg ataacatttc ttcaggtaaa aatatcaaag
     8521 ttcaaaatgc gttgataaag caatcctgga gattagacaa ggtagaagta aattggagat
     8581 agagcaaaaa attcttatct atagtttgaa agttattgga tgttggattt agatttttct
     8641 atctcctttg cccatttaga attactgaaa acaccgaatt tgtagatatc attttgtatt
     8701 gttataacca ctactttttt taagaggaat ccttctcctt ttgatgcttt aatttctacc
     8761 aatggaatat tcagtaataa ctcacctgtt cgtttggaaa gagttgctag agttccttca
     8821 gttttttcaa aaagtaaccg ttgatttgtc aaggttaata gtccattctt taagtgatct
     8881 tcataacaat cctcctgaag aactagtttt tcaccttttc ttggttgtac tgaactcaca
     8941 tttttattct taatcgatgg atatattatt attgcattat aaaattataa aacgaggagc
     9001 agaagcggat gtctttttaa taccatggta tgaaaatatg gcaatttcaa aattaagaaa
     9061 tcctaaaaaa tataggaata aaatattaga caatttaatc agagggcgac gaactatata
     9121 cgaagcaaag atgttaaaaa atgtcaagac tacaggaata aaaactcctt ttatatattt
     9181 tcttgaccca aaacgggctg aaattataat ggaatatatt gatggggaaa atgtgaaaga
     9241 tatcctttca aaggaaatcg gatatgaaat gggtaaatat gtttctgttc ttcataataa
     9301 gaatataata catggtgatt taactacttc caattttata aaacgtaaac acgatctcta
     9361 tttaattgat tttggcctct ctttttactc tgagcgaatt gaagatatgg gtactgatat
     9421 taggataata aaagaaattt taggtagcgc acacatacac gttttcgagg aaacatatca
     9481 aagctttttg gaaggatact cagcatgtac taattataat ttcagccgaa tattaaaagt
     9541 agtcaaagat attgaagcaa gaggacgata tgcaagagtt gagtaatacg tttaatgtga
     9601 aaatatagaa tatctcgggt tttatattct attaaataag cattcctata caacacatac
     9661 aaatgaatga aatatatttt gtaagtcata atattgataa atattctgaa ataaaatcca
     9721 tattaaataa tagaataaca gatctacatt taaaattttg taagcgtaat attaccgaaa
     9781 tacaagacca gaaaatagaa aaaattgcca tagagaaagc aaaacttgcc tacaatatag
     9841 tcaaaaaacc tataattata gaagatgatg gtttattcat caactcttta aatggatttc
     9901 ctggacaata ctcatcatat gtgttaaaat caataggtaa cagagggatt atacgtcttt
     9961 tgaaaggata taaagataga tctgcatttt ttaaatctat ttttgtatac agcaatggaa
    10021 ttagtgttaa agtcttttat ggaaagatta atggaaaaat atcatcaaca ataaaagatg
    10081 atggctgggg atatgatcct atttttattc cacttaataa taataatgat aacaatagtg
    10141 atacaacatt tgctaaatta agtaaaacga ataaaaagaa tgaattgtct catagaagaa
    10201 ttgctttaga taaatttgta aaatggttca accaaaacaa taagttgaat atatgataaa
    10261 tatcagattg gtcataaaac aaagaaagga gaatacacta taatgttatt ttcctataac
    10321 taatttgtga ctgtaaaatt tattaagtta aataattagt attttttttt tatataaatc
    10381 aattcaaatt agaaaaggaa ataaattgtt tatgtttaat gaaagataat cttctattta
    10441 atagtatcgt actcattata ttaaatagcc actttattat ataaagatga aaaatcgcag
//
LOCUS       DASVEV010000206         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_88458, whole genome shotgun sequence.
ACCESSION   DASVEV010000206 DASVEV010000000
VERSION     DASVEV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88458"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF222_09295"
     CDS             complement(<1..888)
                     /locus_tag="VF222_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7142657.1"
                     /translation="MKDTTTLEIENYINQVNNHLLSFLTGEPSELYQASTHYLKSGGK
                     RLRPIMVIKSCEMFKGNQQDALPAASAVEFIHNFSLVHDDIMDNDNMRHGIPTVHKFF
                     GLPLAILSGDILFSKAFQILSTTNIKSIKDSSLLSMIRKLSSACVDICEGQYKDIQFS
                     HGLYFPSEDEYIEMISKKTAALFKVSCSLGALCSRNATEKDVNNMSEFGKNSGIAFQL
                     IDDLIGIAGHSKETGKAVGNDIREGKKTYPILLSFKKASEVERAQILKVFGKDDCDNV
                     NLKKAIDVISSLQIEKMVRK"
     gene            complement(860..1969)
                     /gene="fni"
                     /locus_tag="VF222_09300"
     CDS             complement(860..1969)
                     /gene="fni"
                     /locus_tag="VF222_09300"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX7142658.1"
                     /translation="MSESNNPEIDLIKSRKRDGIDIPLSHNVQSRFVSNYLEYVKLVH
                     NALPEIDFDEINTSTEFLNYKFGAPIIIDSMTGGTPEALKINSLLGQLAETYGLGMGL
                     GSQRAGLKSSSLAETYSVARKNAPSAFLIANIGGAQLAEGLDIKMIEEIISMIDANAL
                     VIHLNPLQELIQPEGEPKYRGVLEKIEKITASISIPIIVKEVGSGISREVALKLEKAN
                     IDCINIAGAGGTSWAGIEKIRANKVKNDVKEHLGELFWDWGIPTALSLIEVRNSLKLP
                     LIASGGIRNGLEIAKCIALGANMGAMAYPFLKAASDSKESLFTFSTNLINELKGAMFL
                     TGSSNIADLAKSRLILTGELADMVINNEGYHHIRD"
     gene            complement(1975..2775)
                     /locus_tag="VF222_09305"
     CDS             complement(1975..2775)
                     /locus_tag="VF222_09305"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HEX7142659.1"
                     /translation="MENRIVIIKLGGSVITFKDKPLSANYKAINNISKVLSKLETPLI
                     IVHGGGSYGHYWSVKWDMHTKPQKYNPHGISIVHSSMLTLNNIIIDKLYTHGLNPYGI
                     SPFSFLIKGKPNSNKILELKKMTEHNIIPVTYGDAVYIKNNKYSILSGDVLMTILSKI
                     LNPSKIIFALNVDGLYNNMRDKKIIKEIKFENDETSKKKPTKINPTNNVNIYDVTGGI
                     KRKVEEASKIAETGKDVIFINGLDSTQIQKAAQDKNFKGTIFKGKIYR"
     gene            complement(2782..3777)
                     /gene="mvk"
                     /locus_tag="VF222_09310"
     CDS             complement(2782..3777)
                     /gene="mvk"
                     /locus_tag="VF222_09310"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEX7142660.1"
                     /translation="MRSTTASAPAKIILFGEHFVVYNQPVILASINKRMKIECKIEVE
                     KNNVVIIETNYFGKKIYPLSIIRNNNHHIENEFFYPLIYILRKILPRDVEPYSINIKI
                     HSEIPYGVGLGSSAALSVAGVAAILGLYYRSKQKNEILDIAIETERILHKNSSGADCV
                     VSTYGGLLYYQKNSKARALNLSKELSFIIINTGLKHSTGELVSLVDRFRKNNLLEFDN
                     ISNRVSRICERAIKALEEGNLLNIGKLMNENQILLDRIGVSTPEINNIINLSLQYGAI
                     GSKLTGAGGGGCVLSLLYPEDKDYFVVKMKKHGYECFPVIIENEGLKVNIESSNF"
     gene            complement(3790..4635)
                     /gene="amrB"
                     /locus_tag="VF222_09315"
     CDS             complement(3790..4635)
                     /gene="amrB"
                     /locus_tag="VF222_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7142661.1"
                     /translation="MRDLTRRSPYYAGSFYPKDKRDLISLLDFCFKDSIFAPKQNQKI
                     NNKIYGMIVPHGAYIFSGAISANAFLEIASRNIDTFILIGPDHKGIGKRISIISKGYW
                     QTPLGDILIDNTITNDLKKSCDFIQEDERAHQIEHSLELQIPFIQYSSRNPFTIVPLL
                     LRNQDIQTSILLGNLIAKFISKKNAIIIASSCLNHYESNLTTYQKDFQMIKSIENLDI
                     ESFYQTIMDLNISVCGYGAIASLMRAVQLLGSKKGILLKYATSGDVTSDDKNTVVGYA
                     SIVFI"
BASE COUNT         1464 a          846 c          680 g         1813 t
ORIGIN      
        1 ttttcttacc atcttttcaa tttgaagtga agaaattaca tctattgctt tttttaaatt
       61 cacattatca caatcatcct taccaaaaac cttcagaatc tgggctcttt ctacttctga
      121 agcttttttg aatgagagta aaatgggata cgtctttttt ccttctctaa tatcgtttcc
      181 tactgctttt ccagtttctt tagaatggcc tgcgattcca attaaatcat caattagctg
      241 gaatgcaata cctgaatttt taccaaattc actcatatta tttacatctt tttctgttgc
      301 atttctcgag cagagtgctc ccaatgaaca ggagactttg aatagagcgg ccgttttttt
      361 cgaaatcatc tcaatatatt catcttcaga tggaaaatat aggccatgag agaattggat
      421 gtctttgtat tgaccttcac atatgtcaac acatgcagag gaaagctttc ttatcatgct
      481 tagcaaagaa ctatctttta tggatttaat gttagtagtg gataggattt gaaaagcttt
      541 tgagaaaaga atatcaccgg ataggattgc aagaggcaaa ccaaaaaatt tatgtacagt
      601 tggtattcca tgtctcatat tatcattatc cattatatca tcatggacta aagaaaaatt
      661 atgaataaac tctacggcgg aggcagcagg taatgcatct tgttgatttc ctttgaacat
      721 ttcacaagac tttatcacca ttattggtct aagacgtttt ccaccacttt ttaaataatg
      781 agtggaggct tgatataatt ccgaaggctc ccctgttaaa aaagataaaa gatgattatt
      841 aacctgattg atgtaatttt caatctctaa tgtggtggta tccttcattg ttaataacca
      901 tgtctgctaa ttcaccagtt aatataagtc ttgatttagc taaatctgca atatttgaag
      961 atccagtaag aaacattgct cctttgagtt cattaattaa attcgtagaa aatgtaaaaa
     1021 gactttcttt tgaatcagaa gcagccttaa gaaaaggata ggccattgca cccatattcg
     1081 ctccaagtgc tatgcacttt gctatttcaa ggccatttct gattccccct gaagcaatta
     1141 acggtaactt taaagagttt ctaacctcta ttaaacttaa agcagtcggt attccccaat
     1201 cccaaaataa ttcacctaaa tgttctttaa catcattttt tactttatta gctctaattt
     1261 tttctatccc agcccaactt gtacctccag ccccagcaat atttatacaa tcgatatttg
     1321 ctttttctag tttcaaagca acctctctcg aaatacccga accaacttct ttaactatta
     1381 tagggatgct tatacttgct gtaatttttt ctatcttttc taaaacaccc ctgtattttg
     1441 gttctccttc tggttgtatt aattcttgta atggattaag atgaattacc aaagcgtttg
     1501 catctatcat cgaaataatt tcttcaatca ttttaatatc taaaccttct gcaagctgcg
     1561 ctcctccaat atttgctatt aaaaaagccg aaggtgcatt ctttctagct actgaataag
     1621 tttctgctaa agaactactt tttagcccag ctctctgact tcctaatccc atccctaatc
     1681 catatgtttc tgctaattgt cctaaaagtg aattgatttt taaggcttct ggggtcccac
     1741 ctgtcattga gtctatgata attggagcgc caaatttgta atttaaaaat tcagtagaag
     1801 tatttatttc atcaaaatct atctcaggca gggcattatg aacaagtttt acatattcca
     1861 aataattgga tacaaatctg gattgaacat tatgggaaag aggaatatct atcccatccc
     1921 tctttctact ctttattaag tcaatttcgg gattattgga ctcagacatc tgatttatct
     1981 gtatatcttt cccttaaaaa tcgtaccttt aaaattttta tcttgtgctg ctttttgaat
     2041 ctgtgtagaa tctaaaccat taataaaaat aacatctttg ccagtttcag ctattttgga
     2101 tgcctcctct actttccttt tgattccgcc tgtcacgtca tagatattta cattgttcgt
     2161 aggattaatt tttgttggtt tctttttaga ggtttcatca ttttcaaatt ttatctcttt
     2221 gattattttt ttatccctca tattgttata tagaccatct acatttaatg caaaaataat
     2281 tttggaagga tttaaaattt ttgataaaat tgtcattaaa acgtcacccg agagtataga
     2341 atatttgtta ttttttatat aaacagcatc tccatatgtg actggaataa tattatgttc
     2401 tgtcattttt tttaattcca gtattttgtt actattgggt ttgcctttta ttaagaatga
     2461 aaaaggagat attccataag gatttaatcc gtgtgtgtat aacttatcaa ttattatgtt
     2521 attaagggtt aacatagaag aatgaactat agaaattcca tgggggttat atttttgtgg
     2581 ttttgtatgc atatcccatt ttacagacca ataatgacca tatgaacctc ctccatgaac
     2641 tattattaat ggagtttcta atttacttag aactttcgag atattgttga ttgctttata
     2701 gttcgctgac aatggtttat ctttaaaggt aataaccgat ccaccaagtt ttattataac
     2761 tatcctgttt tccaatttgt tttaaaaatt actggattca atattaactt tcaaaccttc
     2821 gttctctata attacaggaa aacactcata cccatgtttt ttcattttaa ctacgaaata
     2881 atctttatct tcaggataaa ggagtgataa tacacagcct cctcctccag cacctgtcaa
     2941 ttttgagcca attgccccat attgcagact caaattaatt atgttattta tttcaggagt
     3001 tgatactccg atccggtcta aaagaatttg gttttcattc attaatttac caatatttag
     3061 taaatttcct tcttctaatg cttttatagc cctctcacaa attctagata ctctattaga
     3121 tatattgtca aattcaagta aattattctt acgaaatcta tctactaatg aaactaattc
     3181 tcctgtagaa tgttttaatc ctgtgtttat aataataaaa gaaagctctt tgctcaaatt
     3241 gagtgccctc gctttcgagt ttttttgata atacaataat ccaccgtagg tagacactac
     3301 acaatctgct ccagaagaat ttttatgaag aatccgttcc gtttcgatcg ctatgtctaa
     3361 tatttcattc ttttgcttag acctataata tagtcctaaa attgcagcta caccagcgac
     3421 acttaaagct gcagaagatc ctaatcctac tccatatggt atttctgagt ggattttaat
     3481 atttatacta tagggttcta catcacgagg taatattttt cttaaaatgt aaataagagg
     3541 ataaaaaaac tcgttttcaa tatgatgatt gttatttctt attattgata aaggatatat
     3601 tttttttcca aaataatttg tttctataat gactacatta ttcttctcta cttctatttt
     3661 acactctatt ttcatccttt tatttattga tgccaatatc actggttgat tatatacaac
     3721 gaaatgttct ccaaataaga taatttttgc tggggcagac gcagttgtac ttctcatttt
     3781 gacttaattt taaataaaaa ctattgatgc ataaccaacc actgtattct tatcgtctga
     3841 tgttacgtct ccactagtag catattttaa taatatgcct ttttttgatc ctaatagttg
     3901 tactgctctc attaaagatg caattgctcc atacccacaa actgaaatat ttaaatccat
     3961 tatcgtctga tagaatgatt ctatatccaa attttcaata cttttgatca tttggaaatc
     4021 cttttggtaa gtagttaaat tcgattcata gtggtttaaa cacgatgatg caataataat
     4081 ggcatttttc tttgaaatga attttgcgat taagtttcca agtaaaattg aagtttggat
     4141 atcctgattt cgcaataaaa gtggaacaat ggtgaatgga ttacgacttg aatattgaat
     4201 gaatggaatt tgaagctcta acgaatgttc aatttggtga gctctttcat cttcttgaat
     4261 gaaatcacat gattttttta aatcattagt aattgtatta tcaataagga tatctcctaa
     4321 gggagtctgc caatatcctt tacttatgat agagatccgt tttccaattc ccttatgatc
     4381 aggtcctatt aagatgaagg tatcgatatt tcgagatgca atctcaagga atgcatttgc
     4441 agatattgct ccagaaaata tataagcacc atgaggaaca atcattccat atattttatt
     4501 attaattttt tggttttgtt ttggggcgaa tatactatct ttaaaacaaa aatcaagtaa
     4561 gctaatcaaa tcacgtttat ctttgggata aaaggaacct gcatagtaag gagatctacg
     4621 tgtaagatct ctcaaactat ctcttcaaca agttttgttt caaaatcatc tattgttact
     4681 ttcatttctt tctcggattt tattttccct tgttttttaa gtacttctct agctaataac
     4741 caatatactg tagctagtgc ttttctccct ctgttattag cgggtattac caaatcgact
     4801 ttg
//
LOCUS       DASVEV010000207        11256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1254434, whole genome shotgun sequence.
ACCESSION   DASVEV010000207 DASVEV010000000
VERSION     DASVEV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254434"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF222_09320"
     CDS             complement(<1..225)
                     /locus_tag="VF222_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7142662.1"
                     /translation="MDTFDCIATKLDIREYSKQKVPLEIIKKILEAARLTGTGLNTQH
                     WRFIVVEDKDNLNKLAEDSTSGKWVTDANFA"
     gene            588..749
                     /locus_tag="VF222_09325"
     CDS             588..749
                     /locus_tag="VF222_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142663.1"
                     /translation="MSSIDSKGENEYSDDESNIVGKSGEEGEEQVSDRIKKEIRKDTD
                     IRTQENTPQ"
     gene            965..1291
                     /locus_tag="VF222_09330"
     CDS             965..1291
                     /locus_tag="VF222_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142664.1"
                     /translation="MCTTFFEIYSTGRTPQASPEIIDSYVTGRTIIPKFIKRSEIKII
                     IFDLFAKQIVDKILGSFEEVSESYGVMFIKRYIMPTNYEQRQHMMMMFYYRNKFTLLY
                     DLMRFL"
     gene            complement(1412..1918)
                     /locus_tag="VF222_09335"
     CDS             complement(1412..1918)
                     /locus_tag="VF222_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7142665.1"
                     /translation="MSFKIRNFFSLKKIKESNKNVKSYTNAEEWIIKGENYANRNNLK
                     EAIKCFDMAFNLDHKSDFALGDKALMLDKIKKYDEALSTFSKALEINPNNPITWHNKG
                     LTFARLNKLEESIYCFNNAIDLKQDYAKAWYNKGRCLEKLGELEESQKCLTMAKKIDP
                     FLFTKIKR"
     gene            complement(1903..2175)
                     /locus_tag="VF222_09340"
     CDS             complement(1903..2175)
                     /locus_tag="VF222_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142666.1"
                     /translation="MKIQLNPNIPNLHDNKNILSKLVFLSYHIASYKTISESMGFNSQ
                     DISVDEIYNFIQDLKLDVNSRVPEVDRMEISFCFNILKSMGICHSK"
     gene            2348..3106
                     /locus_tag="VF222_09345"
     CDS             2348..3106
                     /locus_tag="VF222_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7142667.1"
                     /translation="MKIKNIPLNLIDISDENVRKNFSFGKDIDDNLIKLHLSKFELLQ
                     PVVVRFDSASKRYKLLIGRRRYLALSNTGEKEIPAVISDLEGPEAEAASLFENLVRKD
                     LSPVEKATMVKKLVDSTKSGISGISKKYGLPKSTISEWISILTLSKELRDNIENGQIT
                     SYEAIQIARKPKSIQEELAKTRNQNELHEEMIRLGVKRGAPKGLMTVRLVFNPMKKAD
                     KNIWDTLNEKANENGLEVTDYVKTIVANHIRNNK"
     gene            complement(3203..3658)
                     /locus_tag="VF222_09350"
     CDS             complement(3203..3658)
                     /locus_tag="VF222_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7142668.1"
                     /translation="MFKKILFAFDGSATSLKASKYAIHLSVLEHSDLIFLHVIEHIKQ
                     GGVIGLRAKYGDVKLIEGYIRTRKKNAEKWVQPILKEATDKGIKARLDIVEDDGTSLV
                     GVIVNYIEKNETDLVVIGTRGLSKFKRLLMGSVASGVINHSKCPVLLVK"
     gene            complement(3671..3952)
                     /locus_tag="VF222_09355"
     CDS             complement(3671..3952)
                     /locus_tag="VF222_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142669.1"
                     /translation="MSVTTSTQIEEASKKAIESMYGSNIQDFKVRVYFPYTTDPILDR
                     SNSADSRIDSWDVQVTFLLEENQYTVDLIILKKSGQIIYSRLIDTMIPL"
     gene            4127..5833
                     /gene="thsA"
                     /locus_tag="VF222_09360"
     CDS             4127..5833
                     /gene="thsA"
                     /locus_tag="VF222_09360"
                     /inference="COORDINATES: protein motif:HMM:NF041082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HEX7142670.1"
                     /translation="MATQQLFNDGVRRTKGKDARKLNLLACKLLTNLVKNCFGPGGKE
                     KMFIDILGEVTLTKNGCTFLRKIDVEHPAAKVLIDASNSVDNEVGDGTTTVVLLAGGL
                     LEKAEELLEMTFSPTTISNGYHMGLEIALKVLNEISKRVDNSDINILKKIVGTCLRTK
                     IHSFSFDDCNNHITELIVEALCTIRDRDKNEIDIYDIKIEEKPGNVSDTELVTGIVID
                     KTIDSSAMPRNIKNAKILLIDDNLETMRTKTDAQIIGNSYSDLQNFVKAESEIIMNKV
                     QNIIKSGANVVISRKGINLRACEYFAKAGIISIKRAKENDLIWLEKATGAKIANDVNV
                     SELEKFLGFAGKVYEKLVGTDKMVFINECINPKSVTLLLRANSKIFIDELHRAVLSAL
                     TVANGFIIKPQIVGGGGACEAVIASKMKKISHTISKREQIVIQKFAEVLEEIPLTIAY
                     NLGMNVIDSQTKLRYKIHNSNKKHNNVKWYGINALRRNVEEIFDSIIESSIVKQQVLN
                     TAVEVSSLLLGIDDMLMAKPTLDTHTHDNGTVHSHDGGEKKHDHYFDKLGKQQRPIHH
                     YY"
     gene            complement(6131..6520)
                     /locus_tag="VF222_09365"
     CDS             complement(6131..6520)
                     /locus_tag="VF222_09365"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX7142671.1"
                     /translation="MIHIEVRLLEELFPSLFHIFEFPDKDDDEIIFFILIQKIQTKLS
                     MNLKINVNEALIIYCSFIVNKLRAKTCVSDIQEKAKRLLSYKNVMIGVPETLKNTLFI
                     VQVDNIPKTRIILKEPIKIMQYLLKEN"
     gene            complement(6520..7635)
                     /locus_tag="VF222_09370"
     CDS             complement(6520..7635)
                     /locus_tag="VF222_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX7142672.1"
                     /translation="MEFYGDSYKGNDEPIFVGIPTFLKLPWVRNKEELKKVKPDMAIV
                     GEPFDFGTTIRPGARYGPRAIRAASTVPSPPYEHFNIETGVDPFGVFKTVDYGDVVVS
                     PGDVIESHRRMTAKVKEILDINAVPIILGGDHSITFANVRALAKKYKNIGMIHIDCHA
                     DCAPQGLCGFKYDHGAHIRRIMELGCLKGKNYTLIGPRGYWPGPDLYKWMADQDFQWF
                     TMLDVEELGIDQIAKEAADRANDNVDAVYVSWDIDTFDPAYAPGTGEPEPNGLTSREG
                     MRLMRLLSTSFDPDKFAFDLVEVSPTYDVSDVNSYNGGITSGLANRLIVELMAGLSLT
                     KKGEKEGFPIRPKFYRGKGHTYDFGNGPKANIPKRNK"
     gene            7848..9365
                     /locus_tag="VF222_09375"
     CDS             7848..9365
                     /locus_tag="VF222_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142673.1"
                     /translation="MVIDSYGIAVLSFMALVLTVGILTSFLVKKSSKRYMVAGKSLPL
                     FFVGTMLSAQSIDGNSSLGNVALVYQFGFWAGAVIPIGLALCLILTGIIYAPRLNKMS
                     MLTLPDFYYRRYGVGAEGISSVILMISFIILVAGNFAAGGFILSTVLHISLFWAMLIV
                     SCAVLVYTFAGGLFSSAYTDIFQVYLTVGAFWAAFIFFALGFSGVDFGTILGSAPEGF
                     LDLSGLISMENGALAVWGGILALGLGDVVALDFMERVFSARDGKTARRGALMAAGLTI
                     SIIIPVSLMGIVALYFLPNLEDPYTAYPTLAIEYLPFPIGAALLMGVLGASMSTANGG
                     LLAISSVIARNLIQRDILRTILKRPGLDDKKLLITTRLITIPIMLSGFILGFMIPRPG
                     IYLILAFDIVFAGAWAPLTLGLFWKKANWAAAIASLIVGSSLRLMMYFITPPEYAGVD
                     TLLPPPISFVVFIIVALLTQKKYPGKERHGVINYVPPEEDVINGEDLKGYISSKG"
     gene            9470..9991
                     /locus_tag="VF222_09380"
     CDS             9470..9991
                     /locus_tag="VF222_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142674.1"
                     /translation="MPSKGYATVGLKPAILTKLQKQTDEYYPGMFLPSALIIIMNEIK
                     RGFYSAEMHQIKIDFSGGYTSLTIRADVKIWLKENYEILKEEYIEKYQIKNFTQFASL
                     FMLNMFESKSKTNRYMIKLKESDFLWLEEEYKKRNHEYREKYEIYNFDQFADHFIKDI
                     FDKLNSAKQILNW"
     gene            complement(10105..10281)
                     /locus_tag="VF222_09385"
     CDS             complement(10105..10281)
                     /locus_tag="VF222_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142675.1"
                     /translation="MIIEGLTIVIIIGSCALVAWYAKNKFQPEIEKTIEDQKKREIKS
                     YPQIIKKQDLKKNR"
     gene            10894..>11256
                     /locus_tag="VF222_09390"
     CDS             10894..>11256
                     /locus_tag="VF222_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7142676.1"
                     /translation="MIPLNEESLVNAIEKVSKNVVNIASVRMVTDELFRIFPIEGVGS
                     GITIDNQGHILTNYHVVDHARRLKVTLYDGRTFNAKVIGTDKLTDLAVLKIEHDSNNS
                     SNSNNNTNNSDNKAIDLIP"
BASE COUNT         3984 a         1638 c         1741 g         3893 t
ORIGIN      
        1 tgcaaaattt gcatctgtta cccattttcc gcttgtacta tcttctgcca gtttattgag
       61 attatcttta tcttctacaa ctataaatcg ccagtgttga gtattaagac ctgtacctgt
      121 tagtctagct gcttcaagga ttttttttat tatttctaga ggcacttttt gtttactata
      181 ttctcttatg tcaagtttgg ttgctataca atcaaaagta tccatgtttc aatactaaca
      241 taattaataa caaacgtttt aaatatttaa gaaactaatc gggagaagaa ttaaattatt
      301 aaataaattt tactcaactt gaataagaac tctcatataa cagattaaaa ttagataggg
      361 gtttcagtct attgattttt caaaaataaa gataagaatc acttattaga gtagagacct
      421 attattttat attatagaaa tagtatcaga ttatcttata taataattat aaatatttta
      481 caattaaaat gaaacacatt gcaatgaata ttttcaatag ttccttaaaa tagttatata
      541 agaagcaatt aaaaaattaa aaactgatta ttatataata aaaactaatg tcatcgattg
      601 atagtaaagg agaaaatgaa tatagtgatg atgaatcaaa tattgttgga aaatcgggtg
      661 aggaaggaga ggagcaagta tcagacagaa taaaaaaaga aatcagaaaa gatactgata
      721 ttcgaactca agagaatacc ccccagtaga ttaaataaat ctaaactttc gttgcattcc
      781 tgaaatatat tatcaattgg attattcttt acctattcat tttcgaaagc atcatttctt
      841 ttaatttagt taatttacag aaatgtttat ctgattaact aaatatataa agacatgaag
      901 taaaaagcat tattgttcgt tctgatgatc tttctacagt gactaatgtc tccttctaca
      961 taaattgtgt acgacatttt tcgaaatata tagtacagga agaacaccac aggcttctcc
     1021 ggaaatcata gattcatatg tgactggaag aacaattatt ccaaagttta ttaagcgatc
     1081 agaaattaag atcattatat ttgacttatt tgcgaaacaa atagttgaca aaattttagg
     1141 cagtttcgag gaagtatctg aatcatatgg cgtgatgttt attaagaggt atataatgcc
     1201 tacgaattat gaacaaagac aacatatgat gatgatgttt tattatcgaa ataaatttac
     1261 tttattatat gatttaatga gatttctatg acaaaatatt atatatatta caactatgtt
     1321 tatttcattt aatacattac aataatttat tatttatatc tttatgatac atatctttaa
     1381 aggtagtcat ttaatagtca tttatctaaa attacctttt tatttttgta aataaaaacg
     1441 gatctatttt ttttgccata gttaggcact tttgagattc ttctaattcc cctaattttt
     1501 ccaaacacct tcctttattg taccacgctt ttgcataatc ttgtttcagg tctattgcat
     1561 tgttaaagca gtaaatagac tcttccagtt tatttaacct agcaaatgtt aatcccttat
     1621 tgtgccaagt tataggatta ttaggattta tttccaaggc tttagaaaaa gtactcaagg
     1681 cttcatcata tttctttatt ttatctaaca ttagggcttt atcacctaat gcaaaatcac
     1741 ttttatggtc aagattaaat gccatatcaa aacacttaat tgcttctttc aaattatttc
     1801 gattagcata attttctcct tttataatcc attcttctgc atttgtatat gactttacat
     1861 ttttgttaga ttctttaatt ttcttaagag aaaagaaatt tcttattttg aatgacatat
     1921 tcccatcgat tttaatatat taaaacaaaa actgatttcc attctatcta cttcaggaac
     1981 tctactgttc acatctaatt tcaaatcttg gataaaattg tatatttcat caacggaaat
     2041 atcttgactg ttaaaaccca tcgactcact aatagtttta tatgatgcaa tatgatatga
     2101 caaaaatact aattttgata aaatattttt attatcatgt aaattaggta tatttgggtt
     2161 taattgtatt ttcatttttc tataattcct ttctaattga tataaatatt atcaaataag
     2221 acgtattata cgaatttcct ataaaaatca atccgtataa tacgattatt acatttcgtg
     2281 aataattaca tctaatttta gcgtcaaaat gtaatgttaa tatacaattc tagattacta
     2341 attgtatatg aaaattaaga atataccact taatttaatt gatatttcag atgaaaatgt
     2401 gagaaaaaat ttttcttttg gaaaagatat tgatgataat ttaattaaat tacatttatc
     2461 aaaatttgaa ttgcttcagc cagtagttgt acgttttgat tctgcgagta agagatacaa
     2521 attattgatt ggtagaagaa gatatttggc tttatctaac acaggagaaa aggaaatccc
     2581 agcagtaatt tcagatttgg aaggtccaga agcagaggca gcatcattat ttgaaaatct
     2641 tgttcgtaaa gatttatctc ctgtagaaaa agcaacaatg gtaaaaaaac ttgtggattc
     2701 aactaaatca ggaatttcag gaatatccaa aaaatatggg cttccaaaaa gtacaataag
     2761 cgaatggata agcattctaa ctctatctaa ggaattaagg gacaatatag agaacggaca
     2821 gataacatcc tatgaagcta tacagattgc tagaaaacca aaaagcattc aagaagaatt
     2881 ggcgaaaact cgaaatcaga atgagttgca tgaagagatg ataaggttag gtgtaaaaag
     2941 aggtgctcca aagggattga tgactgtaag attggtcttt aatccaatga aaaaagctga
     3001 caaaaatatt tgggatactc ttaatgaaaa agctaatgaa aatggtttag aagtaacaga
     3061 ttatgtaaaa actattgtcg ctaatcatat tcgtaataac aaataattat aacatactat
     3121 catactgatt tattaatagt tcaacagtaa aaataattag agtttactag aaatagaaaa
     3181 atagattgtt atgagataat aattatttga ccaagagaac cgggcatttt gaatgattaa
     3241 tcactccaga tgctacacta cccattaata atcgcttaaa cttggataaa cctcttgtgc
     3301 ctattactac aagatcagtc tcattttttt ctatatagtt tactataact ccaactaagg
     3361 aggtaccatc atcttctact atatcaagtc tggcttttat tcccttatca gtagcttctt
     3421 ttagtattgg ttgaacccat ttttctgcat ttttttttct agttcttata tatccttcta
     3481 ttagtttaac atcaccatac tttgccctca atcctataac tcctccttgc ttaatatgtt
     3541 ctataacatg tagaaaaatt aaatctgaat gttcaagaac tgaaagatgt attgcatatt
     3601 tagacgcttt caaagaagta gcagaaccgt caaaggcaaa aaggattttt ttaaacatac
     3661 ttataaaaaa ttagagtggt atcattgtgt caataagcct tgagtatata atttggccgc
     3721 ttttcttaag aattattaaa tctacagtat attggttttc ctccaatagg aatgttacct
     3781 gcacatccca tgaatctatt ctagaatcag cagaattact tctatcaagt atgggatctg
     3841 tagtatatgg aaagtagact ctaactttaa agtcttgtat attggaacca tacattgatt
     3901 caatggcctt ttttgatgct tcctctatct gagtagatgt tgttacagac atttattaat
     3961 aaattaatta gtaagtataa aaaagtattg attaaacata ttttttaaaa tacattaatt
     4021 cgtaaaatac aaaaatttct ttataggaga aattaataat ttctgtaaac tacgaattaa
     4081 tgaggactac atatattctt cttcataacc aggtagatta tcaaatatgg ctactcaaca
     4141 gttattcaat gatggagttc gacgtaccaa gggaaaagat gcaaggaaat tgaatttgct
     4201 agcgtgtaaa ttacttacaa atctagttaa aaattgcttt ggtccaggtg gaaaagaaaa
     4261 gatgtttata gatatacttg gagaagttac attaaccaag aatggttgta catttttacg
     4321 taaaattgac gttgaacatc ctgctgcaaa ggttttgatt gatgcctcaa attcagttga
     4381 taatgaagtt ggagatggca ctacaactgt tgtattatta gccgggggac tattggaaaa
     4441 agctgaagaa ctattggaga tgacattttc accaacaaca atttcaaatg gttatcacat
     4501 gggattagaa attgctctca aagtacttaa tgaaatttcg aagagagtag ataacagtga
     4561 tattaacatc ctaaaaaaga tagtaggtac ttgcttgcga acaaaaatac actcattttc
     4621 ttttgatgat tgtaataatc atataaccga attaattgta gaggctcttt gtactatcag
     4681 agatcgagat aaaaatgaaa tcgatattta tgatataaaa atagaagaaa aacctgggaa
     4741 tgtttcagac acagaattag tcacaggtat cgtaattgat aagaccattg atagcagtgc
     4801 aatgcctagg aatattaaaa acgctaaaat cctgcttata gatgataacc tagaaacaat
     4861 gagaacaaag acggatgcac aaattattgg aaattcatat agtgatcttc aaaattttgt
     4921 gaaagcagaa tcagaaatta taatgaataa agttcaaaat ataataaaat ctggtgcgaa
     4981 tgttgtaatt tcaagaaagg gcattaatct cagagcctgt gaatattttg ctaaggcagg
     5041 aataatatca attaaaagag caaaggaaaa tgatcttata tggcttgaaa aagctaccgg
     5101 tgctaagata gctaatgatg taaatgtctc tgagttagaa aagttcttag gatttgcagg
     5161 taaagtatac gaaaaattag ttggcactga taaaatggtg tttataaatg aatgtataaa
     5221 tccaaaatct gttaccttat tgctcagagc caattcaaaa atttttattg atgagcttca
     5281 tagggcagtt ctaagtgccc tgacagtagc aaatggtttt attattaaac cacagatcgt
     5341 aggaggagga ggagcctgtg aagcagtgat tgcatctaag atgaagaaaa tatcacatac
     5401 aataagtaaa agagaacaaa ttgtaatcca aaaatttgcg gaggtattag aagaaatacc
     5461 attgaccatt gcttataatt tgggaatgaa tgtaatcgat tctcaaacga aattaagata
     5521 taagattcac aatagcaata aaaaacacaa caatgtaaaa tggtatggaa taaacgctct
     5581 aagaagaaat gtagaggaga tatttgatag cataatagag tcttctatag tgaaacaaca
     5641 agttcttaac acagcagttg aagtttcttc gcttttacta ggtatagatg atatgttaat
     5701 ggcaaaacca acacttgata cacatactca tgataatgga acagtacatt cccatgatgg
     5761 gggagagaag aaacatgatc actattttga taaattagga aaacagcaac gtcctataca
     5821 tcattattac tgatttttta actcattttc tttaaaaatc tactaagaat tggtcaattt
     5881 aagaaattac tgatttagaa tggatttgca gcttatgttt tttatcaaaa tgacagtaac
     5941 tttttctttt cccttttact aaatcttaat agtgattttc gatgtcagat atcaaatata
     6001 ttcaaataac aaatgtaata aatttccatt caaatctgat taataaatga agaatttatt
     6061 gaatagaaaa attattactc tcaaaatcct atagtcattt catttctaca ataatgatga
     6121 taatttttct ttaattttcc ttcaatagat attgcatgat tttaattggt tcttttagta
     6181 ttattcgtgt tttaggaata ttatctactt gaacaatgaa taaagtattt ttaagcgtct
     6241 caggaactcc aatcatgaca tttttatatg acaatagtct ttttgccttt tcttggatgt
     6301 cacttacaca ggttttagca cgaagtttat ttactatgaa tgaacaatag attatcagtg
     6361 cttcattaac attaatttta agattcattg aaagtttggt ttgtattttt tggattagaa
     6421 taaaaaaaat tatttcatca tcgtctttat caggaaattc aaaaatatga aataaggaag
     6481 gaaataattc ctccaataat ctaacttcga tgtggatcat tatttattcc ttttaggaat
     6541 gttagccttg ggtccattac caaaatcata tgtatgacct tttcctctat aaaatttagg
     6601 tctaattggg aaaccctctt tttctccttt cttcgtcaat gacaagccag ccataagctc
     6661 aacgatcagt ctattggcta atccagatgt gattccacca ttatacgaat taacatcact
     6721 tacatcataa gtgggagaaa cctctacaag atcaaatgca aacttatcag gatcaaaaga
     6781 cgtagataac aatctcatta atcgcattcc ttctcttgac gttaagccat tgggttctgg
     6841 ttcacctgta cctggtgcat aggcaggatc aaaagtgtct atatcccaac ttacatatac
     6901 agcatcaaca ttatcgttgg ctctatctgc tgcctcctta gctatctgat ctatacctaa
     6961 ttcctcaacg tccaacattg taaaccactg aaaatcttga tctgccatcc atttgtaaag
     7021 atccggtccg ggccaataac ctcgaggacc gattaaagta taattcttcc cttttaaaca
     7081 tcctaattcc atgattcttc ttatatgtgc accatgatca tatttgaaac cacaaagtcc
     7141 ttgtggagca caatcagcat gacagtcaat atgtatcatt ccgatattct tatatttttt
     7201 tgctagtgcc ctgacattag caaatgtaat tgagtgatct ccgcctaaaa taattggaac
     7261 tgcatttata tctaatattt ctttcacttt ggcagtcatt cgtcgatgcg attcaattac
     7321 atcccctggt gatacaacaa catccccata gtcaacagtt ttaaaaacac caaaaggatc
     7381 tacacctgtt tcaatattga agtgctcata aggaggtgaa ggtactgtag aagctgcacg
     7441 aatagctcta ggaccatatc gagcacctgg acggattgtc gtcccaaaat caaatggctc
     7501 tcccacaatt gccatgtcgg gttttacttt ttttaactct tctttgtttc taacccatgg
     7561 tagtttcaag aatgttggaa ttccgacaaa tataggttcg tcgtttcctt tatatgaatc
     7621 accataaaat tccattccca tatttattca ataataatga ttactattta ttattaaata
     7681 ttcctaagta gattacgttt agacttataa ttttcggcag tcgaaagttc gacagtcgaa
     7741 cattttttaa ttgttcttaa tgtaatctac gtatcaataa gaattaattt tattttttta
     7801 taaacataga gtttatataa ttattaaatt attactaact aagattggtg gttatagatt
     7861 cttatggtat agcggtttta tctttcatgg ctttagttct tactgttgga atattaacat
     7921 catttcttgt caaaaaaagt agtaaaagat atatggtagc gggtaagagt cttcctcttt
     7981 tttttgtagg tacaatgctc tcagctcaat caatagatgg aaattcatct ttaggaaatg
     8041 ttgcattagt ttatcaattt ggtttttggg caggcgcagt aattcccata ggcttagctt
     8101 tatgtttaat tttgacaggc attatttatg caccaagatt aaataaaatg tccatgctga
     8161 ctctacctga tttttattac agacgatatg gtgtaggagc tgaaggaatt tcttcagtaa
     8221 tattaatgat aagttttata atattagtag caggaaattt tgcagctggt ggatttatcc
     8281 tctctactgt tttacatatt agcctatttt gggcgatgtt aattgtttct tgcgctgtac
     8341 tagtttacac ctttgctgga gggctattct cttcagcata tacagatatt tttcaagttt
     8401 atctaacagt gggagcattc tgggcagcat tcattttttt cgctttgggt ttttctggag
     8461 tagattttgg cacgatctta ggatcagcac ctgaaggctt tctagatcta tctggactaa
     8521 tcagtatgga aaacggcgct ttagctgttt ggggaggaat tttggcactt gggttaggag
     8581 acgttgtagc attagatttt atggagagag tattttcagc aagggatgga aagactgcaa
     8641 gaagaggagc acttatggct gctggtttga caatttcaat cattatccct gttagtttga
     8701 tgggcattgt tgccttgtat ttcctaccta atctcgaaga tccatatact gcatatccca
     8761 cgttagcaat agagtatttg ccatttccca taggagctgc actattgatg ggagtgttag
     8821 gagcatccat gtcaactgca aatggaggat tattggctat atccagtgtg attgccagaa
     8881 atcttattca aagagatata ctgagaacta tattaaagcg accagggtta gacgacaaaa
     8941 aattgctgat aactactcga ttaattacaa tccctataat gctatcagga ttcattttgg
     9001 ggttcatgat acctagacct ggaatatact tgattttggc ttttgatata gtttttgctg
     9061 gtgcgtgggc tccactaact ttaggtttat tctggaagaa agccaattgg gcagcagcaa
     9121 tagcgtcttt aatagtaggg tcatctttaa ggctgatgat gtattttata actcctccag
     9181 agtatgcagg agtagatact ttattaccac ctccaatatc ttttgtagta tttataattg
     9241 tagcactatt gacacaaaaa aaatatcctg gcaaagaacg acatggcgta ataaattatg
     9301 taccacctga agaagatgtt atcaacggcg aagatttaaa gggatatata agctcaaaag
     9361 gataaatata aaggttaaaa cctaaatcac aaacatgata taaaagtttc aaaatattaa
     9421 ttactttttt ttataaaaca ataataataa gatcatatct acaaatccga tgcctagtaa
     9481 aggatatgcc acagtaggat taaaacctgc tattcttacc aaattacaga agcaaaccga
     9541 tgaatattac cctggtatgt tcttacctag tgcattaata ataataatga acgaaatcaa
     9601 aagaggtttt tattcagctg aaatgcatca aataaaaata gatttttctg gcggatatac
     9661 ttctttaacc attagagcag atgttaaaat atggttaaaa gaaaattatg agatactcaa
     9721 agaagaatac atagaaaaat atcaaataaa gaattttact caatttgcaa gtttgttcat
     9781 gctaaatatg tttgaatcta aatcaaaaac aaatagatac atgattaaat tgaaagagtc
     9841 agacttcctt tggcttgaag aagaatataa aaaacgaaat catgaatata gagaaaaata
     9901 tgagatatat aattttgatc aatttgcaga tcatttcatt aaagatattt ttgacaagtt
     9961 aaattcagca aaacaaattt tgaactggta aaaataaaaa ttttaaaaac cctctttata
    10021 agtattgtaa tatattatca tttttagaaa ctatttaatt tcaatataca attatgcttt
    10081 caaggataaa taaaaatttg gaatttatct gttttttttg agatcttgtt tctttataat
    10141 ttgtgggtat gatttaattt ctcgtttctt ttgatcttca attgtttttt caatttcagg
    10201 ttgaaattta tttttagcat accatgctac caaagcgcaa cttcctataa tgataacaat
    10261 tgttaatcct tcaattatca taccaaacct atagaggtca atctaatata taattaattt
    10321 ttttgaatat aattaaaaaa ataatcaaaa acctataaaa tattcatgca aaaatagaaa
    10381 tatttgtaaa taataatgtt tatctctgga aatttattaa ttttataatt ctgtaaatgc
    10441 acatatgcaa ggtcaaagtt tgactgaaaa gtattaacaa tattttttac aattctatag
    10501 agttgtttga gagagatcga ttgaattgtg ttatgatttt ctattgatat tggttagttg
    10561 tattgatatt ttctcaagaa tcgctttatt tatgattatt tcttcttcca actatcatca
    10621 aagatatgta tgttaatgtc attgggttta agtcaaaccc tgtatttata ggactatggt
    10681 cctacaaata attattaaat ttgaagatta ttaaaatgtg agctctttat acaagtagtg
    10741 tgactaattt aataataatt aatcaattta atcatcaatc tctattataa tataaaatat
    10801 gtataataga acaatcaatc aaccaacggt aaagaaacca cctataactc cttccaaacc
    10861 aaaatctcca ttagaaagag gagatagaga atattgattc ctctgaatga agaatcactg
    10921 gttaatgcta ttgaaaaagt tagcaagaat gtagttaata ttgcaagtgt cagaatggta
    10981 actgatgaac tcttccgtat atttccgatt gaaggtgtag gatctggcat tacaattgat
    11041 aatcaaggtc atattcttac aaattatcat gtagtagatc atgctagaag attaaaagta
    11101 actctatatg atgggagaac attcaatgct aaagtaatag gaacagataa acttactgat
    11161 ctcgcagttt taaaaattga acatgattct aataattcca gtaatagcaa taataatacg
    11221 aacaattctg acaataaagc aatagactta attcct
//
LOCUS       DASVEV010000208         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_285045, whole genome shotgun sequence.
ACCESSION   DASVEV010000208 DASVEV010000000
VERSION     DASVEV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285045"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VF222_09395"
     CDS             <1..869
                     /locus_tag="VF222_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142677.1"
                     /translation="QPLRLGANSLDLDKYFNGEVDELRVWNRGLAINELSEIYTNRNS
                     TTNGQVEYLNFGKNIMEDNKERIDDDNNKSITNTTKEELLTTLGTTKQPPVIFVFVIS
                     KDILQELLTATAKQPPIIVEPKTPPPSTVTNNNSKTNTTTEELLATTTKKQQQQPPII
                     VEPKTSNQITTAPTLLSNNISKNTADNVRDKINLAFENLSVERSVTNSVVIKGDIKNN
                     STQDLQEVKVFAEYYDKTGTLLAKIDHFITSPSSILKPTEQVSFDILEVLGFGFHKLG
                     DYNIVASGETIN"
     gene            complement(1390..2757)
                     /locus_tag="VF222_09400"
     CDS             complement(1390..2757)
                     /locus_tag="VF222_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142678.1"
                     /translation="MSKLSDIVQFSINDIKLLLLALIISSIISSILVYLLTSTFNLEL
                     EFMFSNWVIDISAGFAVIFALIIIIRERNRKNEGKKYVSLFIAILLWFSAEIVYTYYQ
                     SILRIDVPYPSYADSLWLLGYIFMAYHLYSSFYYWNKKKKFSESSVFIITIFSALLIL
                     FLVQSTAITYSNDINLILVAILYHIADGIILIPALVLLWNLRDEKFLYIHIALISLFV
                     ILNTFANVAYIFTFNSGINIITYYAWIWDLLYNLSYILLAGALFWYDKLIQILNKKID
                     QIFIVNKKQFQFLWEKQDKAEIIGNAYSYIDKENIKDTLNTLITNAKNEIALLIFIQK
                     QYNHNLIANLNLLLTDSKNSNNLKKRILFDTIFNLKLLLSRKPATLDIQYVKIDKILR
                     SDMIIFIIDHQHLLFIDLKKDMSPNNLLAIYTANSNIILQFSSLFENLLNLSELRERS
                     SKYDE"
BASE COUNT         1243 a          417 c          373 g         1111 t
ORIGIN      
        1 atcaaccact tagactagga gcaaattcct tagatttaga caaatatttt aatggagaag
       61 ttgatgaatt acgagtttgg aatagaggat tagcaataaa tgaattaagt gaaatatata
      121 ccaacagaaa ttccactact aacggacaag tagagtatct caattttgga aaaaatataa
      181 tggaggataa taaggaaaga attgatgatg ataataataa aagcataact aatactacaa
      241 aagaagaatt actaacaaca ctaggaacaa caaagcagcc tccagttatt tttgtatttg
      301 ttatttcaaa agatatttta caggaattac taacagcaac agcaaagcaa cctccaataa
      361 ttgtagaacc taagactcct cctccttcta ccgttactaa caataatagc aagactaata
      421 ctacaacaga agaattacta gcaacaacaa caaagaagca acaacaacag cctccaataa
      481 ttgtagaacc taaaacttcc aatcaaatta ccactgcgcc aactctttta tcaaataata
      541 tttctaagaa tactgctgac aatgtacgtg ataaaataaa tttagcattt gaaaatctga
      601 gtgtagaaag aagtgttact aattcagttg taataaaagg tgatattaag aacaatagta
      661 cacaagattt acaagaagta aaagtttttg cagaatatta tgataaaact ggaactttat
      721 tagcaaaaat agatcatttt attacttctc cttcctcgat attaaaacca actgagcaag
      781 tatcttttga tattttagaa gttcttggct ttggctttca taaacttggt gattataata
      841 tagttgcaag tggtgaaact attaattaaa aaataaattt attcacatca ctttataaaa
      901 tcgtgctctt ctcttatagt tcaggtacat attgatggta gatgaagaag attaaagaaa
      961 taaaaaacct ataataaatt tatcatcata aatcatatct ttatttatat ttataaattt
     1021 aataataata aatcaacgtt aggtaacggt ttattagaat ctctgtaatg aactttttac
     1081 tgttatcttt tccttctatt catttgtcta ttcaaatttc atattacaaa caaaaaataa
     1141 atctataatt tatttaatta tgatttgtat acaaaccaat ttacatactc tatattattt
     1201 tgattgtaat acaacataga atcttaaatg tagtaaacct atgtattagt taagttcaaa
     1261 atcccgctga gcccaaattc aaaatggtta atcctagttt ttataattat tcccatgaac
     1321 ctgtggtaat tacaatcatt ccttgaagag ttactagtat aatatagata atataataat
     1381 aatatttcat tattcatcat atttagatga tcgttctctt aattctgata gatttaataa
     1441 gttctcgaat aagctagaaa attgaagaat aatattacta tttgccgtgt aaatagccaa
     1501 gagattatta ggactcatat cttttttcaa atctataaaa agcaaatgtt ggtgatctat
     1561 tataaatata atcatatctg atcttaatat tttatctatc tttacatact gtatgtccaa
     1621 agtagcaggc tttctagata acaaaagttt taggttaaat atagtatcaa ataaaattcg
     1681 ttttttaaga ttattactat tcttagaatc agtcagtaat aaatttaagt ttgcgattaa
     1741 attatgatta tattgttttt gaatgaatat taataacgca atttcatttt ttgcattggt
     1801 tatcaaagta tttaatgtat cttttatatt ttctttatct atatatgaat aagcatttcc
     1861 tattatttca gctttatcct gtttttccca taaaaattga aattgttttt tatttactat
     1921 gaaaatttgg tctatttttt tatttagtat ctgtattaac ttatcatacc aaaataatgc
     1981 tccagcaaga agaatataag aaaggttata taacaaatcc caaatccacg cataatatgt
     2041 aataatattt attcctgaat taaaagtgaa aatatatgct acatttgcaa atgtattcaa
     2101 tattacaaat aatgatatca atgctatatg aatatataaa aatttttcat cgcgtaggtt
     2161 ccataacaaa acaagtgctg gtattaatat tatcccatct gctatgtgat ataatatagc
     2221 aactaatatt aaattgatat catttgaata tgttattgca gttgactgaa ccaaaaaaag
     2281 aattagtagt gctgaaaaaa tggtaattat aaatactgaa ctttcactaa atttcttctt
     2341 tttattccaa tagtaaaaag atgaatacaa atgataagcc ataaaaatat atcctaataa
     2401 ccataaacta tctgcataag aaggatatgg tacatcaatt cttaatatgc tttgataata
     2461 tgtatataca atttcggctg aaaaccaaag taatattgca ataaataacg atacatattt
     2521 ttttccttcg tttttacgat ttctctctct tattatgatt atgagtgcaa atattactgc
     2581 aaatcctgca ctaatatcga ttacccaatt actaaacata aactctagtt caagattaaa
     2641 ggtagaagtt aaaagataaa ctagaattga tgaaataatt gaggatataa ttagtgccaa
     2701 taacaataat tttatatcat taatggaaaa ctgcacgata tctgatagtt tactcatata
     2761 atgacaattt aacctctaaa atcaatataa ataagttaat aatatatctt atttattaat
     2821 taaataattt tatactttac aaatttttta accttgctct agtcaaatta ttaaacacga
     2881 aggagaaata ctgaaaatca agtttaagga atacaagtaa ttttttgcta acataacaaa
     2941 ttaaataata cttgaatcct cttttatttc aaatatgttt cacaacacac taacccagtt
     3001 ataggtgatt catatagtgg gaattgataa tgtatcttgt aatagaagat ggaagatcta
     3061 ttaattaata ccattgaaat tatcaataac gattggaata tttctaatta taaaatatgc
     3121 gaaattataa aataattgat taaa
//
LOCUS       DASVEV010000209         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1254622, whole genome shotgun sequence.
ACCESSION   DASVEV010000209 DASVEV010000000
VERSION     DASVEV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254622"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VF222_09405"
     CDS             <1..898
                     /locus_tag="VF222_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7142679.1"
                     /translation="ITPLLISGAFAERIKYSAFFIFAIAWSLFIYFPVAHWIWGGGWL
                     AQLGVWDFAGGIVIHTTAGMAALAAGLVFGRRKNFGPQIMVPHNLPLAVIGATMLWLG
                     WFGFNAGSALAANGVAGNALLTSQIAAATCTLVWLFLSWKRSGKPSIAAVINGAIAGL
                     AGVTPTAGYISGESAFILGIVLGFGSYYGIVLFKEKLKIDDALDVSSVHGVTGIIGAL
                     AIGVAASPLIMPNGPVGWLHGNPEQLGIQAFGIAVVAAFAFVVSLAIFKIIDKTIGLK
                     VSDEEEEAGLDITQHAEKAFND"
     gene            1066..1602
                     /locus_tag="VF222_09410"
     CDS             1066..1602
                     /locus_tag="VF222_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX7142680.1"
                     /translation="MPIDPDFPKNHQVIGKIQLSDGEHFHYMWGPGKPGEAFENEEVK
                     AAYAARSEEQVPLGVSGTMVAVDWDSCVADGACIEACPVQVFQWFRTEKDVPAKDVTK
                     ETFEGTGSAVKEERKDYTDKADPIREHDCIWCMACVSVCPPQAIKVDQAALEFHEKAE
                     GTYNESISKGGAPPPHAH"
     gene            1760..2362
                     /locus_tag="VF222_09415"
     CDS             1760..2362
                     /locus_tag="VF222_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142681.1"
                     /translation="MVVVTDSFMGIFTPIDISSRITQFIEKKIEFPFIKKNELVGIFY
                     LFGKDNKVISDLEILSVRNIAKRTMEQQARNIKIFHEFKENKLDSEFIREEYNKRILQ
                     ISIEEQISSSSYTHLSYRIAGDPTIQSNCFEQHIAYYKQDYFYELFKQPFKKTEVPAH
                     LINKLEKRMLMLCYNVSDSSKLPYNNTVHPFFQWLNDYNK"
     gene            2666..3100
                     /locus_tag="VF222_09420"
     CDS             2666..3100
                     /locus_tag="VF222_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HEX7142682.1"
                     /translation="MEQHQTKADYELLLDRLHNKLGTTAKKEVSRLELPVPQIIWVGQ
                     RTIFRNFVDFPKALRREPEKLLLYLNKELASAGYINGDRVIFLGRKVRSSFGALIDRY
                     VKDYVICPVCGSPDTRTEKNKKLGFLLCEACGAKSSIKGNYA"
     gene            complement(3280..3699)
                     /gene="trxA"
                     /locus_tag="VF222_09425"
     CDS             complement(3280..3699)
                     /gene="trxA"
                     /locus_tag="VF222_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7142683.1"
                     /translation="MPVTEDNEISAIMKRKKEILEEKLRSMNEIKKINKPVNLTDSNF
                     DTEKSKYSLLVVDFWAAWCGPCKMISPIIEQLAEQYAGKIVFGKINVDANPHISQRFG
                     IQSIPTLMIVKEGQVIDVMIGALPKGQIENRIKQLIE"
BASE COUNT         1261 a          559 c          638 g         1266 t
ORIGIN      
        1 tattactcct ctattaattt ctggtgcatt tgctgaaagg atcaagtata gtgcattttt
       61 catatttgcc atagcttgga gcttattcat ctacttccca gtagctcatt ggatatgggg
      121 aggcggatgg ttagcacagc ttggtgtatg ggactttgca ggtggtatag taatacatac
      181 aactgcgggt atggccgctc ttgctgctgg acttgtattt ggaaggagga agaatttcgg
      241 accacaaata atggtccccc ataatttacc actagcggta ataggagcta caatgctatg
      301 gctgggatgg tttggattta atgctggtag tgcattagct gctaatggcg ttgcaggaaa
      361 cgctcttttg acatcgcaaa tagcagctgc aacatgtaca ttagtttggc tatttctatc
      421 atggaaacgt tcaggaaaac cttccatagc agccgtcata aacggtgcta tagctggact
      481 tgcgggggtt acacctaccg ctggttacat tagtggagaa tccgctttta ttctgggtat
      541 cgttctagga tttggatcat actatggtat agtattattc aaagaaaaac taaagataga
      601 cgatgcatta gatgtcagtt ccgttcacgg tgtgacaggt atcataggtg ctttagcaat
      661 aggtgtagca gctagtcctt tgataatgcc aaatggacct gtaggatggc tacatggaaa
      721 tccagaacaa ctaggtatac aggcatttgg aattgctgtg gttgcagcct ttgcgtttgt
      781 tgtatcctta gcaattttca agattataga taagactatt ggcctaaaag tatcagacga
      841 ggaagaagaa gcaggcttgg atataaccca gcacgcagaa aaagcattca atgactagtt
      901 caaaaatttt tttctttttt ttttattaaa ttaaaataaa ttaatttata atctacatga
      961 tcgtagctag cagcaactaa gcaagggttg ttaaatatat atagaaaccg tttttaattc
     1021 gcatatgatt tatataatat atgttcatgg ataaaataga tataaatgcc aatagatcca
     1081 gactttccaa aaaatcatca agtaattggt aaaatccaac tttctgatgg tgaacatttt
     1141 cactatatgt ggggaccagg taaacccggt gaagcattcg aaaatgaaga agtaaaagca
     1201 gcctatgctg caagaagcga agaacaagtt ccattaggtg taagtggtac catggtagcc
     1261 gttgattggg attcttgtgt agctgacggt gcatgtattg aagcctgtcc agttcaagta
     1321 tttcaatggt ttagaacaga aaaggatgtt cctgctaaag atgtcactaa ggaaacattt
     1381 gaaggaactg gctctgcagt aaaggaagag agaaaagatt atacagataa agcagatcct
     1441 atcagagaac atgattgtat ctggtgtatg gcgtgcgttt cagtttgtcc accacaagca
     1501 attaaagtag atcaagctgc gttagaattc catgaaaaag cagaaggaac atacaatgag
     1561 tcaatttcaa aaggaggcgc tccaccacca catgctcact aaatcttttt tttttattta
     1621 acacacaatg aattattcta gtttatttta aagattattt taggttaata tatcattaat
     1681 atatcatcat attactattg atcaattgtt aataacttat aataacttat tatatgaagt
     1741 attatcttag gagataataa tggttgtagt aactgattcc tttatgggaa tatttactcc
     1801 aatagatata tcttctagaa taacacaatt tattgaaaaa aaaattgaat tcccctttat
     1861 taagaaaaat gagctagtgg gaatatttta tttgtttgga aaagataata aagtaattag
     1921 tgatttagaa atattatccg ttagaaatat agcaaaaagg actatggaac aacaagctag
     1981 aaacattaag atctttcatg aatttaaaga aaataaactt gattccgagt ttataagaga
     2041 agaatataac aaaagaatat tacaaataag catagaagaa caaatatcct cgtcttccta
     2101 tacacatcta agttatagaa tagcaggaga tccaacaatt caatccaact gttttgaaca
     2161 gcatatcgca tattataaac aagattattt ttatgaacta tttaaacaac cctttaaaaa
     2221 aacagaagtt ccagctcatc taatcaataa actggagaaa agaatgctta tgttatgtta
     2281 taatgtgtct gattcttcaa aactacctta taacaatacc gttcatccat tttttcaatg
     2341 gttaaatgac tacaataaat aaaatttatt tctgctatag ataccttttt tcttttatat
     2401 cctctgattc acttaaacaa aagaattttt cagatttaac aattacatat taatagttat
     2461 taattaataa taagtgaatt tttctataat gttcctatgc tgattgtata actactttat
     2521 ttaaaaatgt aataaaattc acctaatgat atgtacttgt tgtattataa tttattagta
     2581 ttattaatat ttcaatagtc taaaatctta tttttatata gttaccacaa cgtacattaa
     2641 taagaaaaag tgtaaattga ttattatgga acaacatcaa actaaagcag attacgaatt
     2701 attacttgat agacttcata ataaattagg aacaacagct aaaaaagaag tctctagatt
     2761 ggaattacct gttccacaaa taatttgggt tggtcagaga acaatattta gaaactttgt
     2821 ggattttcca aaagcattac gtcgcgagcc agaaaaatta ttactatatc ttaataagga
     2881 gcttgcttcg gctggttata ttaacggtga tcgagttatt tttttaggac gtaaagtacg
     2941 ttcgtcgttt ggtgcgttaa ttgatagata tgtaaaagat tatgtcatat gtccagtttg
     3001 cggtagccct gatacaagaa cagaaaaaaa taaaaaatta ggctttttat tgtgtgaggc
     3061 ctgtggagca aaatcttcta taaagggcaa ttatgcataa ttaatcatta ttatacctaa
     3121 atctcataat atgaaaaatt taataagatc gtatttcgat aattttttgc atgagtaaga
     3181 tcgtgactga acgttatgat ctctatttcc tcatggatat aattttatca gtaaaaaaat
     3241 tattttcatt ttattttcta aatatattta agcgtacatc tattctataa gttgttttat
     3301 tctattttca atttgtcctt ttggtaacgc gccaatcatt acatcaataa cctgtccttc
     3361 tttcacaatc attagagttg gaatactttg aattccaaat ctttgtgata tgtgaggatt
     3421 cgcatctaca tttatcttac caaatactat ttttccagca tattgctccg ctagttgttc
     3481 tatgatagga gatatcattt tacaggggcc acaccaggca gcccaaaaat ctacgactaa
     3541 caaagagtat tttgatttct ctgtatcaaa atttgaatca gtgagattaa caggcttatt
     3601 aatttttttt atttcattca tcgatctcaa tttttcttct aggatttctt tttttctttt
     3661 cataatagca gaaatctcat tatcttctgt aactggcata attttttttt tgttatacta
     3721 tatt
//
LOCUS       DASVEV010000210         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_480616, whole genome shotgun sequence.
ACCESSION   DASVEV010000210 DASVEV010000000
VERSION     DASVEV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480616"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VF222_09430"
     CDS             complement(<1..469)
                     /locus_tag="VF222_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142684.1"
                     /translation="MSAKENTTKFNNDNNNFNERTNNTLNQQQDAMNKTFDNTLNNIK
                     RTTDEATREIPRYTQRIAEYQEQTIQTVKDIASDFIEAEKQVIGSFQSQVDRNSNGVW
                     DLYNPQRIAENHAAMVNHFTSYLLNTSNLINNALASNMRVYNTALEQTRDNLKA"
     gene            complement(1129..1563)
                     /locus_tag="VF222_09435"
     CDS             complement(1129..1563)
                     /locus_tag="VF222_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142685.1"
                     /translation="MKNNFFTVRWLDFRQGQGIYLGFLIYFADSILIQYALLIGKFPF
                     IDSLLGANIIGFAIVFIALYVPLAIIIGYWHRKSQWTVEVEALFKENKVGAMMWLFVI
                     DLVEGKINEKEKKEMREMLLKITKGKGKSTPEDSNENTRVEK"
     gene            complement(1592..1849)
                     /locus_tag="VF222_09440"
     CDS             complement(1592..1849)
                     /locus_tag="VF222_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142686.1"
                     /translation="MSSNSTIYNPEDIIKKEARGLGEDTDFGEVQEVGVEYIVTQKGI
                     LDKDKYYIPKNLVDRFEDSIVYFSVTNEEAKQYKEHETNLI"
     gene            complement(2651..2920)
                     /locus_tag="VF222_09445"
     CDS             complement(2651..2920)
                     /locus_tag="VF222_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142687.1"
                     /translation="MGNRSRTEIVAMILDAANEGGETKTKIMYFAFLSYNQLKEYLSI
                     LIENNLIEYLDGANKYKTTEKGLFFLKMYNELGELLPQTTTIKND"
     gene            complement(3528..3800)
                     /locus_tag="VF222_09450"
     CDS             complement(3528..3800)
                     /locus_tag="VF222_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142688.1"
                     /translation="MQYNNMFMVLPVTTTLILISTLLSMAIFTNVSVFAQSEGTNQTG
                     ESLKQNASDIVSNISKEVKSKGSNITKEVAKDVAANIGKQLQDLAK"
     gene            4295..4600
                     /locus_tag="VF222_09455"
     CDS             4295..4600
                     /locus_tag="VF222_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142689.1"
                     /translation="MKDCFSAGDLSNIASTYIGIIIGAFIGGFVSWLIYNRQEKTAVI
                     QKELQDHILNLEEKHEISLEKHENILEKIQIVEQNHDEMLNKILTLDKKINSLLESK"
BASE COUNT         1715 a          622 c          603 g         1767 t
ORIGIN      
        1 aagcctttag attatcacgt gtttgttcta gtgctgtatt gtatactctc atatttgatg
       61 ctagagcatt atttattaga ttacttgtat ttaacaggta acttgtaaaa tgatttacca
      121 ttgctgcgtg attttctgcg attctttgtg gattatacaa atcccataca ccattactat
      181 ttctatctac ttgtgattga aaagagccta ttacttgttt ttctgcttca ataaagtcag
      241 aagcaatatc ttttacagtt tgaatagttt gttcttgata ttcagcaata cgttgtgtat
      301 atcgtggaat ctctcgtgta gcttcatcag ttgttctttt tatattattt aatgtattgt
      361 caaatgtctt attcattgca tcttgttgtt gatttaaggt gttatttgtt ctttcattga
      421 aattattgtt gtcattgtta aatttagttg tattttcttt tgctgacatg tatatatata
      481 agtcactaag gtatataacc gtatgtatta atgtctagtg tatagtcata tttgtttatt
      541 tgtaattaat ttatttattt gtataatttg atgtaatata ttcagaataa caacaataac
      601 aagcagaatt tttagatatt atggacaaat gggagtaata ccgatagcgg ttctaaaaga
      661 ggagtctatt ttagagatat tactacaaag catatagctt ttattattta gagacttatt
      721 ttaaaataat ttttgtttga actttcaaaa accttttaga tatatatttt gaaatgagga
      781 ataataatta aaatcattta gataaaacat aaaaataaaa aattagaggt tatcaaagta
      841 caatactcaa gttaacatac tgaatttcaa tttaaaacta tacaaaagcg agaatgttag
      901 atattatcac tactgttaat aaatataatt tttaatgtat tacaattaat tggaatagta
      961 taatctaaaa aatatgttac ctgtattagt tgaaaaagac caagaatatc caataatgtc
     1021 ataatagagc ttatgtacta tatacattct caaagcttgg tttgatgttt tcatttagta
     1081 ccttttcagg ttcaaataca ttattaatat gtagataata ctaagacttt atttttctac
     1141 acgtgtattt tcattactat cttctggagt cgattttcct tttccttttg ttatttttaa
     1201 aagcatttct ctcatttcct tcttttcttt ttcatttatt tttccttcta ccaagtcaat
     1261 aacaaaaagc cacatcattg ctccaacttt attctcttta aataaggctt cgacttctac
     1321 tgtccattgg ctctttctat gccaatatcc tattatgatt gcaagaggta catataatgc
     1381 tatgaatact attgcaaatc ctattatatt tgctcctagg agcgaatcta taaaaggaaa
     1441 ttttccaatc aacaatgcat attgaataag tatagaatct gcaaaatata taagaaaacc
     1501 aagatatata ccctggcctt gtctaaaatc caaccatcgt actgtgaaaa aattgttttt
     1561 cattatcatt aaactataac taaataaaaa attatattaa gttcgtctcg tgttctttgt
     1621 actgttttgc ctcttcattt gtaacactaa aatagactat actatcctca aatctgtcta
     1681 ccaaattctt tggaatatag tatttgtctt tgtccaaaat acctttttgt gttactatat
     1741 attctacacc aacttcttgt acttcaccaa aatctgtatc ctcccctaaa cctctagctt
     1801 ctttttttat aatatcttct ggattataga tagtagaatt acttgacata taatagtagg
     1861 ataaacattt tatataacta atttagttct atttggttag tggattaact attttatctc
     1921 tttactacta atccaataac ttgtttctca tattcatatt attagagcca atttcaatta
     1981 tctgaaattt tatctatatc ttttctgact gacaagcagt gtctaaatat cgctataaat
     2041 caaatattga gcaaacttta caaatagatt ctgtctctct ccctctctac aaccagattg
     2101 ctaaaataaa tagatgatgt tttgttttct tgtagttgat attattagat aacaagatag
     2161 aatccttacc gaaatttata tcagcaaaaa ctcttagaag ataacttcat tcttgaattt
     2221 tcaaataatg ataaaaatat caagaaacca agaaaaaaaa gaacaaatga actaacaata
     2281 ggtgaaatag cttgttttgt taaacctcat tcagattttg tttgtgatac tattgtaaga
     2341 ggtagattgc caaaagtcca aaagtaaata ctgctgactt gtgcatgata caataacaat
     2401 gaaaaaatat atttgaacta cgaataaaag tagaataaca tactctataa agcagattag
     2461 gaaatggtac ttaaatttct cattgactaa tcatattatt caaaataatg ctgaaaaatg
     2521 ttgaaacagt aaatactagt caatgttcta aaattcatat tataaactta tttttaaatg
     2581 atacgaaaat agagttacta taaaaattat tattatatgt tttaggatat taaatgaaaa
     2641 attagattat ttagtcattt ttaattgtag tagtttgggg taacaattct cccaattcat
     2701 tatacatttt aagaaagaac aatccttttt ctgtagtttt atatttatta gccccatcca
     2761 gatattctat cagattgttt tctatcaaga tagaaagata ttcttttaat tggttatagc
     2821 ttagaaaagc aaagtacata atttttgtct tggtttctcc tccttcgttt gcagcatcca
     2881 gtatcattgc tactatttct gttcggcttc tatttcccat attattagaa atgtgattta
     2941 ctattttaat attaatacca caagaaaagt taacctaact atagtaaaac attcaacata
     3001 ttttttgcaa ataataaata taagaaatgt tcctatctta tctcagaatt gtattatgaa
     3061 ataattatca tatttaattt tatcaacaat cattctctat atataactta attctaaatc
     3121 cgtataaaat aatagggaaa atttcagaaa aaagcaaaat tcagaagaga ggacaataat
     3181 tcaaattaat ttattctata aataggagag agttaagata ctaaatagca gaggctattt
     3241 ttaattttag ttgttcttaa tccagaggtt tgacggtcat ttttgcctgt aatcttaatt
     3301 aatcatttaa attaacaaat tcattgggct cagaggtttc cattgtgata tttattctaa
     3361 aaaaaggatt caaaatatta tttatataat atcagaatca tttaagaatt gaacatcaaa
     3421 ttagtttcta taaaaacctt taaaattttg aaacatttca aaaaagatac aaataagaaa
     3481 gaatcatcat tataaaaaat caccatcatt aaaaagataa agatgaatta ctttgctaaa
     3541 tcttgtagtt gttttcctat atttgcagcc acatcttttg ctacttcttt agtaatatta
     3601 gatcctttgc tctttacttc tttactgata tttgatacaa tatcactagc attttgtttt
     3661 aatgattcac ctgtttgatt agttccttcc gattgagcaa agacagaaac attagtgaat
     3721 attgccattg acagtagtgt cgaaataaga attaatgttg ttgtcactgg taataccata
     3781 aacatattat tgtattgcat tgattctttt aattagtata tataaataaa gttatcgtaa
     3841 tattgttgaa aatcacaaaa ttaatagata attattaaaa atatcaaaaa ttttgtaaaa
     3901 aattattttt gtagagttta acaaaatgta gcaaaataat tcttaacata ataaaaaagc
     3961 ttaaggaagt aatattttct aaataaatga caaataaaat tatcttattc tatgagctaa
     4021 aaaaatgaaa gttattccta ttgctaaatt aataaaataa taaagttaaa ccctgtgcta
     4081 attttgccga ggacggctat cccctgtaaa tatttttcat atggtaaaac tagagaagaa
     4141 aactttgcta acactccctg taaattaccg gaagtgaatg aaactaggta attagttgct
     4201 atttgtttat atagaaatca ataaaataaa aagtaacgaa tcaagttcat gtaatatgtt
     4261 attagaaata ttcataatgt ggggatgtgt cgacttgaaa gattgtttta gtgcaggaga
     4321 tttatccaat attgcttcta cttatatagg tattataata ggagcattca tcgggggctt
     4381 tgtcagttgg ttgatataca atagacaaga gaaaactgcc gttatacaaa aggaattaca
     4441 agaccacata ttaaacttag aagaaaagca tgaaattagt ctagaaaagc acgaaaatat
     4501 tttagaaaag atacaaatag ttgagcaaaa tcatgatgaa atgttaaaca agattttgac
     4561 attagataag aaaataaatt cattgttaga aagtaaataa taataatttc aaagaattaa
     4621 ttttatttct ttctagttaa aggtctataa gatgggtagc aataactgaa caagatggta
     4681 ttattctaaa tgaacaaaat agagaag
//
LOCUS       DASVEV010000211         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_90586, whole genome shotgun sequence.
ACCESSION   DASVEV010000211 DASVEV010000000
VERSION     DASVEV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90586"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF222_09460"
     CDS             complement(<1..767)
                     /locus_tag="VF222_09460"
                     /inference="COORDINATES: protein motif:HMM:NF044564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142690.1"
                     /translation="MVKKELETALKFNGERIYSNIDKDIFLISRRNCDGLVAGTIIST
                     ALLKLDTRFTFRTIDYLDSDTVARIISEDHDFNIFIDLNIEDIGKIKNLKDKFLIIDH
                     NKISQNLKDYEEYLLNPYIHGINGKNEISSGGLAYLIAEKLNKGNKKLSLLAIVSALA
                     EKQDTGVKKSLTGLNSEIASVASSTGIVNIKTDLVFNSLGYKPIHEALAYNISPFIEG
                     VTWNKENCYKILKNSGINTHKNGKLRTFAEISEEEKN"
     gene            complement(774..1223)
                     /locus_tag="VF222_09465"
     CDS             complement(774..1223)
                     /locus_tag="VF222_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7142691.1"
                     /translation="MARTHAHTHGKSHSIRPATKDAPSWINQKPKEIVELIVKLSDEG
                     LSPTEIGLKLRDEYAIPLVKPILGKSITTILKDNNISSTMPEDLNRLLQKALGLQKHL
                     KTHNSDHRNVRSLELIESKIHRISKYYKYNGKIPSNWKYSAVIAQLE"
     gene            1320..1628
                     /locus_tag="VF222_09470"
     CDS             1320..1628
                     /locus_tag="VF222_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142692.1"
                     /translation="MNKPNIWIATAIVMVIGTTFLGLQSAVAQYAGSSNLEEELKLAQ
                     EKVSNAGQEGAYGSGTSMLSADTSQTAIFIVILVAIFGGVAAAFFAMSRSRHKEPAKY
                     "
     gene            1810..2094
                     /locus_tag="VF222_09475"
     CDS             1810..2094
                     /locus_tag="VF222_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142693.1"
                     /translation="MVVGAITNLSAMISNGFNSLIQQIGPNYDPLFYDVMVYIFGTIL
                     FAVFLLGVISFWLRRKGLGGASAKERWTQELEKYFEREQIGLIPDEKHHW"
     gene            complement(3251..3808)
                     /locus_tag="VF222_09480"
     CDS             complement(3251..3808)
                     /locus_tag="VF222_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HEX7142694.1"
                     /translation="MIKSALAVTIGIVIVMSTAVTLIPIKDADGHGVQAQLQSRFVRI
                     QDEAFSAQNLQTGEELTVTGTLQSLVNRPLRAWISLFSESTNAGNRWEFTSRDPPGNV
                     FVLEPQATIPYTVTAKALEPGVYHVHTQLNVATVGPGLGPGQTVQVTGPAILKAVPWE
                     NVFYQVIIIGAGLGVTFATRPWQVI"
     gene            4008..4301
                     /locus_tag="VF222_09485"
     CDS             4008..4301
                     /locus_tag="VF222_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142695.1"
                     /translation="MTLPKGYSSGGKRVDGGNRDEIERMIGRRVEHMNGVYIMGFILC
                     WVATAGGIYVGVTVYPWAYPLPSGMYALSVLTVIEILGVIFIIKVANEKSVRP"
BASE COUNT         1525 a          669 c          705 g         1638 t
ORIGIN      
        1 atattttttt cttcttcaga tatttctgca aaagttctca atttgccatt cttatgagta
       61 ttaatccctg aatttttcaa aattttatag caattttctt tgttccatgt aacaccttct
      121 ataaatggtg aaatattata agctaatgct tcatgtatgg gtttatatcc aagagaatta
      181 aaaactaaat ctgtcttaat atttacaatt ccggtcgatg atgcaaccga tgcaatttcg
      241 gaatttaatc cagttaagga ttttttcact ccggtatctt gtttttctgc taatgcagaa
      301 actatagcta aaagtgacaa ctttttattt cctttattta atttttcagc tattaaatag
      361 gctaatcctc ctgatgatat ttcatttttt ccatttattc catgtatgta tgggtttagt
      421 aaatattcct cataatcctt taaattttgt gatattttat tatgatctat gattaagaac
      481 ttatctttga gattcttaat ttttccaatg tcttctatat ttaaatctat aaaaatatta
      541 aaatcatgat cttcagaaat tattcttgcg acagtatcag aatctaaata atcaattgtt
      601 ctaaaagtaa atcttgtatc taattttaat aatgctgtag atataatcgt cccagcaact
      661 aatccatcac aatttcttct agaaattaga aaaatgtctt tatcaatatt cgagtatatt
      721 ctttcgccat tgaatttaag agcagtttca agctcttttt taaccattat tatttattct
      781 aattgtgcta ttactgcaga atacttccag tttgaaggaa tttttccatt gtatttgtaa
      841 tatttagaaa ttctatgaat cttcgattct attaattcta atgagcgaac attcctatga
      901 tcgctattat gagtttttaa atgtttttga agtcctaacg ctttctgtaa tagtctattt
      961 aaatcttcag gcattgtaga gctaatgtta ttatccttta gaatcgttgt aatggatttt
     1021 cctagtattg gtttaacaag gggaatagca tattcatctc taagttttaa tccaatttca
     1081 gttggcgata atccttcatc agataatttc acaataagtt ctactatttc ctttggtttt
     1141 tgattaatcc aactaggggc atctttagta gccggcctaa ttgaatgaga ttttccatga
     1201 gtatgagcat gagtacgagc catattacta ttaatttttt tgtatatctt aaatgttact
     1261 taattatctc atttcatatg ataaaagtta aataattgaa tttaaaagtg aagattagga
     1321 tgaataaacc aaatatctgg atagcgactg caatagtaat ggtgatagga actactttct
     1381 taggacttca atcagcggta gcacaatatg ccgggagttc gaatttagaa gaagaattga
     1441 aactagcaca agaaaaagtt tcaaatgctg gacaagaagg agcttatggt tcaggtacat
     1501 cgatgttaag tgcagatact agtcaaaccg ctatatttat agtaatactg gtagcaatat
     1561 tcggtggcgt tgcagctgca ttctttgcta tgagtagaag cagacataaa gaaccagcta
     1621 aatactaatc ttttttttta tttgaaaaaa cattcattca ttcagttata agatttaatt
     1681 tctagtgttt ataagaatca tataatttaa aattctgaca aattttccaa cattatatat
     1741 tattttaaaa gtttaatgga atattaaata ccaaaatgta tatatcacag cctttgattg
     1801 ctatatggta tggtagtagg tgctataacc aatctcagtg caatgatcag taacggattt
     1861 aatagtctaa ttcaacaaat aggtccaaac tatgatccat tgttctacga cgttatggtg
     1921 tatattttcg gtacaatatt atttgcggtc ttcctgcttg gggtaatttc attttggcta
     1981 agaaggaaag gacttggcgg agcttctgca aaagaacgtt ggacacaaga attggaaaaa
     2041 tactttgaaa gagaacagat tggtttaatt ccagatgaga aacatcactg gtaaaccatc
     2101 attataatga tggtttgttt aacaaacaaa ccatgtttaa tcttttttta tttgatatta
     2161 attaattttg aaagttatgt ttagtgtact aaatagttta aaatatcatt tattgtagat
     2221 tcgtaacaaa tgccgccatt taaggttgtg taaagtctta gtcagattga aagggctgtc
     2281 aaaaaaatgt caagtactat tctatcattt gatgacaatc acattttgta catttaaatt
     2341 atcaccttga tacaacaaat ggttctttct tagatgaata catcctttac aaatggcata
     2401 ctcatcaagc atttcacccc tttatttcta tcatcaatca aaagttaatc ttgaacactc
     2461 atttttctaa aaagtaaaca gctaaagatg ggttatttta ttaataaaaa aatttagctt
     2521 tacagcaatg tttgatttat tcaaattacg tttaatggta tgtcacgaaa aatgatgttc
     2581 gatagataat ataattataa gggtctgcga aatctatgag accgaacaag taatataact
     2641 atcattatga tacttcattt gatgtgatgt ttttagtggg aatacaatat actaacaaaa
     2701 tttcagaact aacgtacatt aatcatggag gagaaccagg taattctgct ggttttgtat
     2761 gctattggtt ctggaaagag ttacgctaaa attttcctga accaattaca aacgacaaat
     2821 ataactatga gacaatttgc agtaggatat ttcattataa aatataaaat atataaatac
     2881 agaattggat gcttcaattg gtattggaaa tttggtaaaa gccatttaag aatggttgga
     2941 caaagataat ctaattacgt ttaaaaaaag aatttagaaa taataaacct catacaactt
     3001 attccttaac tgaaaagcga atgatgaatt tgctaaatta agagattatt tgaattttta
     3061 gaataattta tccgatggtt ttttacacta ttttgtatct aacctgaagt ttagcaccca
     3121 atttaattac ctaatatata atatataatt atttataata ataactaaag tagcaccatc
     3181 ataaatatta cttccataaa ttaatattta tagagatttt atttaaaaaa taaaaaaaga
     3241 gttggttatt ttaaataacc tgccacggtc tagttgcaaa agttactcca agtccagcac
     3301 caataattat tacttgataa aatacatttt cccatggtac tgcctttaat attgcaggac
     3361 cggttacctg aactgtttga cctgggccta aacctgggcc taccgttgca acattaagtt
     3421 gggtatgtac gtgatatact cctggttcta gggcttttgc cgttactgta tatggaattg
     3481 tggcctgagg ttcaagcaca aagacgttac ctggtgggtc tcttgaagta aactcccatc
     3541 tgttacctgc gttagtggat tcactaaaaa gagaaatcca tgctcttaat ggtctattga
     3601 caagactttg taacgtgcca gttactgtta gctcctctcc agtttgcaaa ttttgtgctg
     3661 aaaaagcttc atcttgaatt cttacgaatc tgctttgaag ctgtgcctga acaccatgac
     3721 catcagcatc ctttattgga ataagagtta ctgcagtaga catcacaata actataccta
     3781 tagttaccgc taatgcactt tttatcatat tcgtatagta ggaaaggtga ataacatgga
     3841 tatatgtttt gctgttttct tgtctttcat ttttaaaata ttttaaaata gtaaaacgta
     3901 taactacgaa taactaaaac caattaattc ttaaatctct ctattctggt cataattata
     3961 caaaggaata gcaaaattta aaagggatat tcagacacta atattacatg actttaccaa
     4021 aaggatatag tagcggtgga aaaagagttg acggtggaaa tcgtgatgaa atagaacgta
     4081 tgataggtcg tagagttgaa cacatgaatg gagtttacat tatgggattt atcttatgtt
     4141 gggtcgcaac tgccggtggt atatatgttg gtgtaactgt atatccgtgg gcttatccat
     4201 tacctagtgg aatgtatgca ttgtctgtac tcaccgtcat agaaatatta ggagtaatat
     4261 ttatcataaa agtagcaaac gaaaaatcag tacgtccata aaaccttttt tttaatctac
     4321 tatacttttt aaaaatcttt tttttagttt taataactcc aatataatca caagatatgg
     4381 gatacaaatt aattcgataa aaaaataaaa ttttatatac aactttcaat tgtaggatgt
     4441 taatggtctg gctacgacgt acaacgcact atctattcat agtggtcgtt gctgttaaca
     4501 gtacattatt gactatcaat gctggtgact atatctt
//
LOCUS       DASVEV010000212         6069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1256121, whole genome shotgun sequence.
ACCESSION   DASVEV010000212 DASVEV010000000
VERSION     DASVEV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256121"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF222_09490"
     CDS             complement(<1..213)
                     /locus_tag="VF222_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX7142696.1"
                     /translation="MRYNEKDKAEQIPGSQNAGETASVVSEQGGIPGQRKEVNVENYE
                     KVASLGQILKDIEFPTNKDKIMQFVKT"
     gene            617..739
                     /locus_tag="VF222_09495"
     CDS             617..739
                     /locus_tag="VF222_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142697.1"
                     /translation="MIGKCQSCGTSNVEVTPSKENGKEKLLCNQCRPADGAYGY"
     gene            1129..1620
                     /locus_tag="VF222_09500"
     CDS             1129..1620
                     /locus_tag="VF222_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX7142698.1"
                     /translation="MASSDTDIIGLETVTADNKNLGKVIAIDVKMKYHYFIVLKKGLL
                     KDEEYHIPINSITNISVNESKITINLNENEVKHGYELLNESNPSSNLIKGKSNSSFSL
                     PFEKDKIKYESFSNNLEEPKTEKSEEVVVDTYICEMCQDKFKDISQFELHRKNKHSGP
                     VGI"
     gene            1661..2020
                     /locus_tag="VF222_09505"
     CDS             1661..2020
                     /locus_tag="VF222_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142699.1"
                     /translation="MTHFHKLFIECIFEWSKKAEQCDSNECLEDSKKRLLDSLDKIFP
                     YSKDIEFEVDKVNYILSSMFFLSNRLAKATIGLAELDKSLENLKQVGNLSFEKKENNE
                     TYNKFVHDLNEILEKYF"
     gene            complement(2286..2690)
                     /locus_tag="VF222_09510"
     CDS             complement(2286..2690)
                     /locus_tag="VF222_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142700.1"
                     /translation="MNKKTYSPSQFGRDAEFIIALYLLSKGWEVKLSKGSKGPADLMA
                     KKDLILLYIQIKASSKLPRIKGSAITKLKSKSFMDGALPVVATLQPMFPLLDHDEKIL
                     NHDSITLSVKKKDKMEFKIYFYDLSDWSNIDL"
     gene            complement(3192..3755)
                     /locus_tag="VF222_09515"
     CDS             complement(3192..3755)
                     /locus_tag="VF222_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142701.1"
                     /translation="MSTKDINNNKNRSETRISKNIVFGILTAGVIVAMGFTGISNISA
                     QSMPNTTTAAAANNDSSQMLGNVNLTGTIDVDKAIVEAFKSKVTINIIDAITTAQNSV
                     GPNSTVKEVELTEAHGYLVYKMKIIGEDLKKYKVLVDPGNGSILFQKEITYSDYDHKM
                     MGKEMGDNYKDGYKHGYDKMKYDKGYK"
     gene            complement(3842..4420)
                     /locus_tag="VF222_09520"
     CDS             complement(3842..4420)
                     /locus_tag="VF222_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142702.1"
                     /translation="MSNEDNKRQNNGQSDNDNTNSIFQIMDRIMKELNKTRKIFVFMF
                     FSMMILPVTTIIIFMYTFDDPKIDRGELRANIQEFNLLSQKLKSQMIQLENLDSSGKQ
                     KELQSLLLNSEEYKQTINRIDELSQNELKLISEEPKNNLHKKEIRLITFILSIIWIIL
                     GIRQYTILSSWGKRYNRFKKQQEEIDKKFDME"
     gene            complement(4469..4768)
                     /locus_tag="VF222_09525"
     CDS             complement(4469..4768)
                     /locus_tag="VF222_09525"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142703.1"
                     /translation="MVHEYRDKTFIRKDILVLLAEYGELNQTKLLSYCGLNLAKHKKI
                     LDEMSEKGLIERNENLWGSKIIVNYKITEKGKEFCKMVLDPYEEMFPRNKREKDE"
     gene            complement(5629..>6069)
                     /locus_tag="VF222_09530"
     CDS             complement(5629..>6069)
                     /locus_tag="VF222_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142704.1"
                     /translation="LDYEEIGFPMDRWHFLFDPTGSQASAAGSGYAGLEEGGSKVVSV
                     FSLGESSFREGTHAAKESSISTTIDGSNVNIKSSDPPPSAQIQIAGFAQIKKISNSEI
                     AFVSPKGPSGTATATGGFPLQVLLILGGMMGAVAILVLIKARKP"
BASE COUNT         2102 a          889 c          792 g         2286 t
ORIGIN      
        1 agtttttaca aattgcatta ttttatcttt atttgttgga aattcaatat ctttaagtat
       61 ttgacctagt gatgcaacct tttcataatt ttctacattt acttcttttc tctgacctgg
      121 aatacctcct tgttcactta ctacggaggc tgtttcacca gcgttttgag atcctggaat
      181 ctgttctgcc ttatcctttt cattataacg cataatatgg tatgtacatc aaaatattta
      241 ttgcttgatt gatttacaag ataaaggtct aaattatgaa ttcataaatt ctgaatgtga
      301 gtaatatcca aataaacata catctctgta tacccttctg aaaaaaactg tattgtcatt
      361 aataaagaaa gttataaact aacaaaacaa aatatatatc tcaacaaata atactataat
      421 tcttaataat attaatacaa taaattatta aaaatcccaa tacggttttg ccttgataaa
      481 taatttaatc tcattgtaac ctttacaata ttatacgatt ggaaaaggtt agtaattaaa
      541 aactaatttt ctttgctata ctactaatta agagaaatac atccttttta ttttatattt
      601 actaaataat tataaaatga taggaaaatg tcaaagttgt gggacatcga acgtggaggt
      661 tacaccttca aaagaaaatg gaaaagaaaa actattatgc aatcaatgca ggcctgcaga
      721 tggagcctat ggttattgat gaatactaat tcaaaaatta acctaaccat tgtgcataat
      781 tttttattta ttgaataatg gtgttagata tataataaag gaaagaaaaa tttggttttt
      841 atacttgttt ttctacaatt tatgaagaat tgaattgctc tcattataac aaaaatttat
      901 ttatattttg ttgtttacag gtatccatta tgaattttac acggttagtt aatatctaca
      961 aaaaaaatct aaaaataatt tcgaaaaaac gcaacctgga cgacctaatt gctttccata
     1021 atagcaggag aacaataaca aataccattg aaccacataa aatgaaaacc aatttaatgt
     1081 atcatacaaa atattttata tttaaaaatt aattggatgg atttagtaat ggcgtcttca
     1141 gatactgata ttataggatt agaaacagtt actgctgata ataagaatct aggaaaggta
     1201 attgcaattg atgtaaaaat gaaataccat tattttatcg tattaaaaaa aggtctatta
     1261 aaagatgaag agtatcatat ccctatcaat tcaataacca atatttcagt taacgaatcg
     1321 aaaatcacta taaatttaaa tgaaaatgaa gtaaaacatg gttacgaact tttaaatgaa
     1381 agcaatcctt cttccaattt aattaaaggt aaatcaaatt cttctttctc actacctttt
     1441 gaaaaagata aaataaaata cgagtctttc tctaataatt tagaagaacc taaaacagaa
     1501 aaatcagaag aggtggtagt tgatacatat atctgtgaaa tgtgtcaaga caaatttaaa
     1561 gatatttccc aatttgagtt acatcgaaag aataagcatt cgggtccagt agggatttaa
     1621 tagggtttaa taataatcca ttttggtgaa tgaaacgaat atgactcact ttcacaaact
     1681 ttttattgaa tgtatatttg aatggtcaaa aaaagcagaa caatgtgaca gtaatgaatg
     1741 cctggaagat agtaaaaaaa gattattgga cagcctcgat aaaatttttc cgtattctaa
     1801 ggacatagaa tttgaagtcg ataaagtaaa ctatatcctt tcatcaatgt tttttttatc
     1861 caatagatta gcaaaagcga ccattggctt ggcagaattg gataaatcat tagaaaatct
     1921 taagcaagta ggtaatttga gctttgaaaa aaaggagaat aatgagacct acaataaatt
     1981 tgttcatgat ttaaatgaaa tattggaaaa gtatttctaa aattatgatc tagtctctga
     2041 cttgcaatgt aaaaaacgat tttcaaacta agagtgatat tctaaaatat atctataaat
     2101 aaaaaatgaa tctttacatt tacaattatt atatattaat ctataacgat gctatataga
     2161 tgagcatcgt tgacttattg aaaatatata ttcaattatt atcattatta ttactaattc
     2221 aagttacaaa gtattgtact attattgttt ttaatacgta ataaatttaa ggattatcca
     2281 ctaatctaaa gatcaatatt actccagtct gataaatcat aaaaatatat cttgaattcc
     2341 attttatctt ttttcttgac tgacaaagta attgaatcat gatttaatat tttttcatca
     2401 tgatctaata atggaaacat tggttgtagc gttgcaacta ctgggagagc accatccatg
     2461 aaggatttgc tctttagttt agtaatagct gaacctttta ttcgaggcag ctttgaactg
     2521 gcttttattt gaatgtataa tagtatcaaa tccttttttg ccattagatc ggctggccct
     2581 ttactacctt ttgataattt aacttcccat cctttggaaa gcagataaag ggctataatg
     2641 aattctgcgt cccgtccaaa ttgacttggt gaataagttt ttttattcaa tattttatgt
     2701 taataaacaa aactgcacta tcatattata gtatcttatg ctatattttt aaaaattttc
     2761 tcacttttaa ttttttatat tctttctgat tgttaccttt attctgaatt taaggatcta
     2821 ttttctgttc tatctggaat tgatttcgcc tcatagttaa agagtctata gtataaagta
     2881 taaagaagga taaaagtaat tagtgataat aatttttatt gatggacact ctaataatga
     2941 acaattttta cttattacaa aaaaaagaaa gaaattgaga tgtgaatttt tataatgaac
     3001 tacttcattt ttttttcaat aacgctatca agcaacacaa tgaattgtga gtaaaaatat
     3061 caaaaaccaa aaaccttttt tataagatag agagtttaat gataattttt atacctaggt
     3121 tattaaatca tatatacaaa aaaaaagtta gatattaaaa tttatttttt ttatattgtg
     3181 gtagtgtggg tttatttata ccctttatca tatttcattt tatcatatcc atgcttgtac
     3241 ccgtctttat aattgtctcc catctccttt cccatcatct tatggtcata atctgaatat
     3301 gttatttcct tttgaaacaa aatactacca tttccaggat ctaccaggac tttatatttt
     3361 ttcaaatctt cacctattat cttcatttta tataccaaat atccgtgtgc ttctgtcaat
     3421 tctacttctt tcactgtcga gtttggtccg acactatttt gtgctgttgt tattgcatca
     3481 attatattga tagtaacttt ggatttgaat gcttcaacaa tagctttatc aacatctatt
     3541 gttccagtca aatttacatt ccccagcatt tgtgatgagt cattattggc agcagcagca
     3601 gtagtagtat taggcatact ttgtgcagaa atattagata ttcctgtaaa tcccattgct
     3661 actattactc cagcagtaag tattccaaat acaatatttt tagaaattct agtttctgat
     3721 ctattcttat tattattgat gtcttttgtt gacatacgat tcaataatat tgatatattt
     3781 taaagttggc taaacattgg cagttaacaa tgttaattta tttatgtatg atatggttga
     3841 tttattccat atcgaatttt ttatctattt cttcttgttg ttttttaaat ctgttatatc
     3901 tttttcccca tgaggataga atagtatatt gtcgaattcc taaaattatc cagataattg
     3961 aaagaataaa tgtaattaaa cgaatttctt ttttatgtaa attatttttg ggttcttcac
     4021 ttattaattt caattcgttt tgagataatt cgtcaattct attgattgtt tgtttgtact
     4081 cttcagagtt caataataat gattgtaact ccttttgttt tcctgaagaa tctaagtttt
     4141 ctaattgaat catctgactt ttcaattttt gtgatagcaa attaaattct tgtatattcg
     4201 ccctaagttc tccccgatca atttttggat catcaaaagt atacataaaa ataattatgg
     4261 tagtaactgg aagaatcatc atactaaaga acataaatac aaaaatcttt ctagtcttgt
     4321 ttaactcctt cataattcta tccattattt gaaaaatact gttggtatta tcattatcag
     4381 attggccatt attttgtctt ttattatctt catttgacat agatggacta gctcaccaaa
     4441 atcctcttaa attctttaat taacaatgtt attcatcttt ctccctttta tttcttggaa
     4501 acatttcctc atatggatca agaaccattt tacaaaattc ctttcctttc tctgttattt
     4561 tataatttac gatgatttta cttccccata aattttcatt tctctcaatt aatccttttt
     4621 ctgacatttc atcaagaatt ttcttatgtt ttgctaaatt taagccacaa taactaagaa
     4681 gtttagtttg atttagttca ccatactcag ccaggagaac taggatatcc tttctgataa
     4741 aggttttatc tctatactcg tgaaccagct tgtttccctc ttaaacagat tcagatttgt
     4801 aaaaatgatt tttatttaac aaaactaaac aacctaataa tcaattcagt aatatattat
     4861 atttatcaca tatatatcca taaacaaaca tgtacattat ctttatgtaa tttacttatt
     4921 tctttcttta tctataggtg tatttatcta agaattcaaa tggttattgc caggcatcgg
     4981 cattttcatt aggagcatag ttatcacctg atggattcgc gaatgattgg tttagtcccc
     5041 acaattcatg aatcataaca atcgcaggca tttttttatc catttttttg ttattatttt
     5101 ttaaaagagg atatgctaag tatcctaata attctcatga taattgacat tctctttcaa
     5161 gattgaaact ggctgtaatg atgatattgt ggaacgtttt attttcctct tctttaattg
     5221 tggacaaagg ttttgcatct acatcaaata aagaaaatta ggtccaaata caattacaga
     5281 aaacataccc aacaacgaaa tacttaatgg aaaacttaac atattaaact accttactta
     5341 ggttaagaat aatatttaac tttaaacaaa tgaatttaaa aatttattgt tcaataaaga
     5401 caatgattaa tgtgatatgt aaccaattaa taacgttaat tttattattc acaaataaaa
     5461 taggtataat ttcttatcgg ttttattttg taaataattg caacaacagc agagcaatta
     5521 tgatgttttt ataaaatctt tattattact ttaaaaacag aagacgataa ttaaatgtaa
     5581 aaaattgtaa gtggtgtgta taattatttt tgagaatatt tttgtatact aaggctttct
     5641 agctttaatt aatacaagaa tagcaacagc acccatcatt cctcctaaaa ttaagagtac
     5701 ctgtagtggg aatcccccag ttgcagtagc agttcctgat ggtccttttg gactgacaaa
     5761 agcaatttct gaattgctga tcttcttaat ttgtgcaaac cctgctattt gtatctgcgc
     5821 gctgggtggt ggatcagatg atttgatgtt aacatttgaa ccatctatag tagtagatat
     5881 agaactttct ttagctgcat gtgttccttc tctaaaacta ctttctccaa gtgagaacac
     5941 tgatactact ttcgatcctc cctcttctaa tcctgcatat cctgaacctg ctgcagaagc
     6001 ttgacttcca gttggatcaa agagaaaatg ccatctgtcc atagggaaac caatttcttc
     6061 atagtctag
//
LOCUS       DASVEV010000213        28137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1646660, whole genome shotgun sequence.
ACCESSION   DASVEV010000213 DASVEV010000000
VERSION     DASVEV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 28137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28137
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646660"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF222_09535"
     CDS             <1..433
                     /locus_tag="VF222_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7142705.1"
                     /translation="VSQYKTNIKEMNNTINLNFIREEKNLFIKADKGRITQVLDNLLC
                     NAIRFTEGIASGSITITAKREENDSIKVITKDTGIGIDKEILSRLFTKFATKSDKGTG
                     LGLYISKKIIEAHNGKIWGKNNDNDDDNGIGAEFGFILPIE"
     gene            890..1063
                     /locus_tag="VF222_09540"
     CDS             890..1063
                     /locus_tag="VF222_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142706.1"
                     /translation="MSSLTKLENSSIRNEWVKKGGNLIEAKPYIQKTYRISAGLLLIV
                     MISLPISYHSNTT"
     gene            complement(1072..2376)
                     /locus_tag="VF222_09545"
     CDS             complement(1072..2376)
                     /locus_tag="VF222_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142707.1"
                     /translation="MTSEEKQIPLYPLLLINFIGTLGFSIVLPFLVFLVIDFGGNAIV
                     YGILAAMYPAFQLIGAPILGRWSDIYGRKKVLLLSHGGTLVGWIIFLCALFLPVENLF
                     SINSALIGTFIITIPLVILFLARALDGITGGNISVANAYLADVSTDANRSKNFGKMAI
                     SSNLGFIVGPAIAGILGATIYKEILPVLAALSLSLVTLIVIVFTLKESKPSASTAAGA
                     VIQVLEKENIGKVFAQECKECYKIVNPKKLTFLDIFKLKHISFLLFLYFFIFLGFNIF
                     YTSFPIHAVVGLKWSITQMGIFYAVLSGMMILVQGPVLRKALQRFSEQKLVIIGSIIL
                     GTNFILLISNNILLIYVAAILFAVGNGLMWPSLLSILSKHGGTVHQGAVQGVASSFAS
                     LASIIGLIIGGILYNLIGGITFLISAGVIFAVFIMSFRLLKI"
     gene            2693..3733
                     /locus_tag="VF222_09550"
     CDS             2693..3733
                     /locus_tag="VF222_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142708.1"
                     /translation="MGENVIFSFHFSFSLKVRFLTFLFVTTIFVISITLNQDQIVIAQ
                     QHQQFLEDISFDIDGVTFSHHMASVNGIQMHYVIGGKGDPIVLLHGFPQSWYEWRHIM
                     PYLAKNYTVVAPDLRGFGDSSKPVTGYDGKTTAEDIYQLTSQLGFNQILLVAHDVGGQ
                     TAYSYAAAHPNNVTKLVIMDFPFPGFLPPEFGENGPWWFAFQQTPNLPESLVEGKERE
                     YISWFFKGLAYNPSAITEEDIDIFANHFSSPGTAHGAFEHFRAFSVDAEQNKESAKTK
                     ITMPVLVLSGDIYPALGGDLPQDMMLSSMQALAQNVQGITVPLSGHWIPEERPDFSID
                     QLFKFFGNSTNK"
     gene            complement(4703..5599)
                     /locus_tag="VF222_09555"
     CDS             complement(4703..5599)
                     /locus_tag="VF222_09555"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7142709.1"
                     /translation="MSLAGLTVAITGSRRASELAHLVTSFGGRPYLAPTVGIEASQDI
                     SKETEFFINKILEERVDYVVFMTGPGVYSLMSAAKNLGIEKKFVEALQKTTVIARSFK
                     PKIALTNHGIKTDIIPEENTAEGIAKLLKSFSMAGRNVAVLWHGSYSHLLKDELHAVG
                     AEVFESSTYRYSLDLKESGARILERMGFKSIPPDEAKVVKLIEDISKGLVDAITFTSP
                     PSVHDLFKIAEAYSLREQLQYTLNKEVIVVAVGPSTMKALDENHVHIDVIPKVFKMGP
                     MIKSLSDYLGQADYPKKKSKPL"
     gene            complement(5590..5793)
                     /locus_tag="VF222_09560"
     CDS             complement(5590..5793)
                     /locus_tag="VF222_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142710.1"
                     /translation="MPTVQTKGNIYIRIKEFVTIIAVVSLDVICKIKNIKAFLPTIET
                     DLIKTTRKNFLMVESTQGDKLCP"
     gene            complement(6091..7344)
                     /locus_tag="VF222_09565"
     CDS             complement(6091..7344)
                     /locus_tag="VF222_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142711.1"
                     /translation="MKKRFCFRRRVSDNHTENQQYSNNNDSTFVASSYFYKRLINRFL
                     VGCVLVSSGILLAESGGSWDITNHLLNKPETFLSPPHVILYSGIGLALVGATIMFLHW
                     RSYSLYISTRKEEKSLELDLSIKLVISGVSMLILAGPLDFSWHSAFGLDGLLSPPHLT
                     LTIGMLAGSIGALIGITSCIVIINNSISPDKRQNNNNDTFKKNEKIPISLYTTLTILG
                     ILPVWMSSSGLIYMFSLPFSDTEYYNFNLEPAIAATIATLTLPFLVSFILVSLFKLVE
                     EIGNKKRKFGIISITGSIFIIITIVTMIIPNKSLISTIPFYLLNIIPIIMADIFLSKL
                     PLKRVFIYASGGILGSSFFMIYYPMITHIYNEVLSSQPLWPSLTAPTYFDMIVQVYPL
                     LAVPAVGAGILGTIVSSRIINRKNK"
     gene            complement(7402..8037)
                     /locus_tag="VF222_09570"
     CDS             complement(7402..8037)
                     /locus_tag="VF222_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142712.1"
                     /translation="MNFDYSERHNSIKRVNITIVLIFITAISLIIYYSLSIFFIPQIY
                     LSSSSEVKSQPVITGAKLSASKVVVGERFDLTITAMNNGDTADMQVVYLSFPNITAID
                     SIVKILKYNFTQKPIFIEIEDEIGSEYVGLDNVINASYPAIEANSRPWQTGDYYQISL
                     EVEPSTVGRFVIFVKTIALPHINELNHYPRSGIKDHQGEFVAVSSVEVVNK"
     gene            8588..9721
                     /locus_tag="VF222_09575"
     CDS             8588..9721
                     /locus_tag="VF222_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142713.1"
                     /translation="MTITIIDYNRYITHLSILYVHAEPNDNNKEQGDSEQKQDKKIEK
                     KEEQDQTKDKDKDEDKDEENSNKNYNSDSDQMDPAYRPFFPIKRIVIPDVYETIPNGQ
                     TYRFNINNPNDKVQVDKTDKSNIFTKQNLDGSWRIDYGKPRIDIHTKDAGILPDKVDQ
                     LNNLSKGKIQSWNYSELKDIGYWYKPTDWKNIEVTLVFKLLDSARSKGDQHALSIVTR
                     SISHSQLDSEYKKSDDEPQFFCGGSSYHNNLSNDGNVRMKKEQFHIDYEWERYNPEIQ
                     VGDIYNKIIGFKAIVYNVNDTAVKLETWVDTQNQGKGPYKKVHEGIDLGNWGDNMKVC
                     GAETDGQAITWGSPIVILKANDFKFDIYDIEVREIIPPLKQIS"
     gene            9931..10797
                     /locus_tag="VF222_09580"
     CDS             9931..10797
                     /locus_tag="VF222_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142714.1"
                     /translation="MSLNKAYVRHLYLFIVISIVSTIVFVSSYDLSFSQKDAKNKEDK
                     DEGKDKLVVKAQINLKNIDLEKTKFIRVIGFINGEESKKDIPISSIDKSENNLDVELK
                     VNEENDIVEASTPDEFFVCAYQVGDVQKQEATTPITKFDCNEGDILGNPTEISLFQSG
                     SQVYSKSQVYYQAHLNTINITNSDTVKIKILAPLADKKDTEKLIIAVMIKGQIKSEVI
                     EDVQGELDKSKGTTIQRTFTFDRDTDIGKIQIGDRYHACVASNDLSPPEGQECEKRLV
                     KNFDKSNSLPAR"
     gene            11098..11631
                     /locus_tag="VF222_09585"
     CDS             11098..11631
                     /locus_tag="VF222_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142715.1"
                     /translation="MTFMSFQEEEKEITDLRRKEQGKTNLSSIDVHIDDYYENVISSS
                     NIFPQKNKVPPLYFDDLINNNLTIKTLKDIKYNFIEEHRNILNTYYSIYSQRLDDIIY
                     YNLDNLMALDNHFDNNNINQNLINNQTNTTKIINDIVDKNMDTFIKSIKFASKFYSDV
                     TKSYCNYIGMIIKSSER"
     gene            11953..14850
                     /locus_tag="VF222_09590"
     CDS             11953..14850
                     /locus_tag="VF222_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX7142716.1"
                     /translation="MSTTVTNKKFEYPGSKRNFSPLYRFTINHMYLKIKPDFKKKQLL
                     DCIQKLTIEALYNIEELVLDIAELDIHNVSSETDNIDIKSFDILYKKDKLVIKFRNMI
                     TNGSKFIIKINYSSGYYSKGKPNGKPRSGFTFIAQDYSSNSTDAIQSWTQGETVESRY
                     WFPCIDNPQMKFAREIVVVAPTEEYDVISNGEYERKGNVWLWKESTPTPAYLTSVVIG
                     KFNTDGFEKNIPPLKYYWPNDISKIDAMRTFADTPKIIKFFEIYLDTKYPYSKYWQVI
                     VDKFEYGGMENTSCTTLTRNILHDERASLDYSRDIVIVAHELAHQWFGDLITCKDWSD
                     IWLNEGFADYFESLYWGHRHTKKYEKKKTNDEFLYQIIQKIDTYMEEASTLYKRPIVT
                     NLYKHPDELFDAHSYEKGGLVLHMLKNFIGEENFKKSIKKYINTYRERTAKTEDFQEI
                     CEKISGMDLNQFFYQWLFTAGHPELDIQIMEEKNKNTRMKKLKVKITQNQSGDFIFVF
                     PLEIRLVYRTNTNEKSSIDKIERIQVNEKETKYTFKEEISANAIIDLISIDPELKILK
                     EIRSLTIEKQNEKFNLLHILKNQLKDQTTTIAERISALKIIKENFYSQEILELLKDIV
                     MKDSVFYAVSVEAANVIGSYHDIKDFNKDKESYEILKECLTNTIFFTLAPQTKRAIIS
                     NIGKFEREDTLELKNENNVPLLVALLDDQSYFVENATATTIGKSIKNLPDVNPLKEEM
                     IKILKDKVNSITFQDQLAQGAINGLSELAQDENIEKIKDIVNLLINKSLERDSVTETM
                     NRYFVRSAATLALGKFLVTKNEKIIDDKNLKERADTLNNKILTHLIQLLKDGEMRRIK
                     RNACTALADSDAKIVQPNERVRKSIDALKKLAEEDVDGFVRRTAEVSLNVIREWLKEW
                     TDTQPKLDINLRKDIDDKIDAKKIGKREKHTDPGNNDEKILKVSRKEVLEY"
     gene            15488..15706
                     /locus_tag="VF222_09595"
     CDS             15488..15706
                     /locus_tag="VF222_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142717.1"
                     /translation="MIDPNINGITEDADIDNRKIAQILLDQFNEFSSIVFLLNNGDVY
                     FDEPFERQINLTTDNLSFRDYYQASETD"
     gene            15880..16209
                     /locus_tag="VF222_09600"
     CDS             15880..16209
                     /locus_tag="VF222_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142718.1"
                     /translation="MIDKNGVKIGKSNENETSISKESFDTKEQFSNLTSVKFELEGKL
                     GSILEKFDGKESQITFLPYEIFQNKRILLLIQSCNSDGNESNKCIENKDHNLVNENVL
                     SKLSSLF"
     gene            complement(16488..17129)
                     /locus_tag="VF222_09605"
     CDS             complement(16488..17129)
                     /locus_tag="VF222_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142719.1"
                     /translation="MELLLYDFFNTLFSEYTYPFVFFIIFVLSVIPILTPPTWIVVVS
                     AYSLNDQLNPLLLSIIGATAAIAGRLLLLQLSTFGRKTINDQRKSSLEKLRKYLEKTR
                     YGYFIGTLLFALLPLPSNMLFISYGLINAKSISIIVGFWLGRFLVYIMMIYVSYYFFN
                     SFKEMLNSDIQTLILMNIVGILMTLFMLLIDWNILLSEHKLSFIKPKRFFSKQ"
     gene            complement(18036..18227)
                     /locus_tag="VF222_09610"
     CDS             complement(18036..18227)
                     /locus_tag="VF222_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142720.1"
                     /translation="MRYKLEEKKSLSCEVCGAKMAAMKATTISVNEFHKDGTIKIHYT
                     CSNHIVELYNKIVKVIENK"
     gene            complement(18472..19599)
                     /locus_tag="VF222_09615"
     CDS             complement(18472..19599)
                     /locus_tag="VF222_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142721.1"
                     /translation="MSCILFFSLSLFKSSYAIKNDNNDEEFDYIFDPGQYIEYEEEEE
                     TTNDNLNNKMSENKKRYESIINKIKDADITKKNNTIINNFVEKPDNNNNSTNYNFIAV
                     GDWYCNEETKKTINNILAINPELIITTGDQVKESPSAACWIQMSEPIKDKMKIAIGNH
                     DAEFANIYKQIVTYHKLTSPYYSHDFRNIHFISMSTEHPFEQGSKQYEFIKNDLEKLS
                     KNSSVDWIVVHQHKPLYSTSQDKGEAEQLRDIYQQLFQQYDVDLVISSHNQYYERTYP
                     LLYNEQYEKATNKKIEPKPIITNTVKSEYSDKDNGIVFLTVGTAGDKLDPVKERYGFY
                     VLQESQFGFLNVKIENNGKTLMGEFHTNNGEVIDYFKLNTT"
     gene            complement(20073..20480)
                     /locus_tag="VF222_09620"
     CDS             complement(20073..20480)
                     /locus_tag="VF222_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142722.1"
                     /translation="MSSKQVFYTISSIILVVVVAITPSYHIQAQKENEENDPLIILTS
                     IKNLLQKSITELNAGNYTGASELVDIAYIDNYEYIEDPLKELDNDLMEETEVMIREDF
                     ASAIEDKKPIEEITTLHNNIKTNLDKAEQLFKQ"
     gene            complement(20976..21989)
                     /locus_tag="VF222_09625"
     CDS             complement(20976..21989)
                     /locus_tag="VF222_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142723.1"
                     /translation="MLNNNNEFTCYTYRISNYIYQISFLSLLVLFFFSLNINNLTIFG
                     QKSDSDFSEHEDINIVSAGDYYCNDETEDTIENIISVDPELIITTGDHVKDEKSAKCW
                     IQMSEPIKDKMKIAIGNHDAEFSKIYKQIIKNHNLKSPYYSHDFKNIHFISLSTEHPY
                     EEGSKQYKFIKNDLKISSTNSSIDWIIIHQHKPLYSTNADIEESERIKDTFLPLFEKY
                     GVDFVISGHNQYYERSYPMSYNKVIGLETKDQESMPELTKDDDDDSKSIYENTNGIIY
                     LTVGTAGDELQQIKKKDAYYIIQKGKYGFLNLKLENSGKTIIGEFHTNNDKILDNFKV
                     KKS"
     gene            complement(22188..22424)
                     /locus_tag="VF222_09630"
     CDS             complement(22188..22424)
                     /locus_tag="VF222_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142724.1"
                     /translation="MIHKISTEQKPQIPNIVFECGDFENDIEMLLIEREGEFHLHLHN
                     SFTDDSMDMKVDIRDFARMFASLSEYFRRDRIKI"
     gene            complement(22533..22715)
                     /locus_tag="VF222_09635"
     CDS             complement(22533..22715)
                     /locus_tag="VF222_09635"
                     /inference="COORDINATES: protein motif:HMM:NF022410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEX7142725.1"
                     /translation="MKPKVKILETQGSAISLESQINQFLQKIDVDNFIDIKLNTLERI
                     NSSPNDSHIALIMYLE"
     gene            complement(23168..23578)
                     /locus_tag="VF222_09640"
     CDS             complement(23168..23578)
                     /locus_tag="VF222_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7142726.1"
                     /translation="MINTGDRITIQELIARYNLAIDNKNLNEWINTWTEDGIWTTTFG
                     EAKGKTELKNMIDQVTNEFASGKRHLSTNIIIEDAQNNMASANSYLTVIEAHNSPEVV
                     ASGTYSDIIKKNNNGEWKFFQRKLDIDLVNESNK"
     gene            24710..24889
                     /locus_tag="VF222_09645"
     CDS             24710..24889
                     /locus_tag="VF222_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142727.1"
                     /translation="MAKKNSIAKALKFALSGFSLFFIGMLFFIVVYDALTGASSETTT
                     EYCAKYGFLASPKCW"
     gene            25204..25338
                     /locus_tag="VF222_09650"
     CDS             25204..25338
                     /locus_tag="VF222_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142728.1"
                     /translation="MMRTFVEWILDSIPHEFQKKGDIECYEKGWNYILTIPRYGIIDG
                     "
     gene            25687..27054
                     /locus_tag="VF222_09655"
     CDS             25687..27054
                     /locus_tag="VF222_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7142729.1"
                     /translation="MINAIEYPPGPKDLLPYSLVRKFLRDPLKMLTTISQTYGDISHF
                     KFGKQNVYFLNDPNYIEEVLVIHYKNFIKSRGLQVSKRLLGSGLLTSEGDYHDKQRRL
                     IQPTFYPKRINSYAEVMIKKTLNLCDNWKEGSVLDIHKEMTRLTLDIISKSVLGYDIK
                     PEDDEIGDSLLTCMEYFNRLLMPFGELIEKIPILPINKGFQKAKRNLDSIVFRMINEH
                     RMKLEKNEKKDNDLLFTLLQAQDDSADTGKMSDQQLRDEVMTIFLAGHETTSNALTWT
                     FYLISEHPEIDERLQKEIYSIVGNKTDLSVDDISKLEYTTKVLTESMRLYPPAWALGR
                     QAINDCKIGKYIIPSGSIILMSQYVMHRNPLYFPEPDTFHPDRWTDEFKKNLPRFSYF
                     PFGGGIRSCVGEPFAWMESILITAIINKLWKMKRIPSNKVVLKPLITLRSKYGMHMKI
                     TRRNY"
     gene            complement(27697..>28137)
                     /locus_tag="VF222_09660"
     CDS             complement(27697..>28137)
                     /locus_tag="VF222_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142730.1"
                     /translation="NIKKTTNEARKEIPRYTQIVNDYQENTIQAIREITDNYVESQKD
                     IIVNSVQLWSDWMSSPRQVAENYGKIVSSFADNAVAATRVLNNTVFANLDSVNTSVQH
                     TKENVKELSTIGANTVKTFQQISSHNIHNNNNNKTTTTASSSNS"
BASE COUNT        10383 a         3864 c         3987 g         9903 t
ORIGIN      
        1 tgtatcacaa tataaaacta atataaaaga aatgaacaat actataaatt tgaattttat
       61 tagagaagaa aaaaatttgt ttatcaaagc agataaagga agaataacgc aggttttaga
      121 taatctattg tgtaatgcta tccggttcac agaaggaata gcaagtggta gtataactat
      181 tacagctaag agagaagaga atgatagtat taaagttatt acaaaggata cagggattgg
      241 tatagataaa gaaatattgt caagactttt tacaaagttt gctacaaaat cagataaagg
      301 aactggatta ggcttatata tatcaaaaaa aattattgaa gcccataatg gaaagatttg
      361 gggaaagaat aatgataatg atgatgataa tggaataggt gcagaatttg gatttatcct
      421 tccaatagaa taggattaaa atttagaata taagcgtaac ttctcatctg atagcatgaa
      481 actaaaaaaa ttaaaaaact atctctagat aaatttgtgt ctactcaaat agatttgact
      541 ataataaaag ttatcttcat tgtagattta tattctcctt tacgagatac ctatcgtata
      601 ttatggcaaa gtaacattaa acaatttctt tagtttttct atatgagaga agctatatta
      661 gctattgaca ctttagcttt taccattccc tatacctcat aaaagtccat attaaaatat
      721 cgacaagaac acctgatatc gcattacttc tagatattcc agccacacaa gatttaaaaa
      781 tttgacatgt tttaaattaa atcccaaaca agatccgttt ttatcatcaa gtatttaaat
      841 agcaatttaa tctaatttga atacattgga atatttgggt ttaataatta tgagttctct
      901 tacaaaattg gaaaactcct caatacgaaa tgaatgggta aaaaagggag ggaatttaat
      961 agaagccaaa ccttatatac aaaaaactta caggattagt gctggtttac ttcttattgt
     1021 aatgataagt ctacctatat cgtaccattc taatactact taatcaaaag cttaaatttt
     1081 taacagtcga aaagacatga taaatacggc aaatattact ccagcgctaa ttagaaaagt
     1141 aattcctcca attaaattat acaaaattcc tcctattatt aatccaataa tacttgcgag
     1201 acttgcaaaa ctacttgcaa caccttgtac tgctccttga tgaacagttc ctccatgttt
     1261 tgatagtatc gacagtagag atggccacat aagaccatta ccaactgcaa acaaaattgc
     1321 agctacataa attaacaaaa tattatttga tatcaataaa ataaaatttg tacctaagat
     1381 tatactacca attataacta gtttttgctc agagaatctt tgtaatgcct tacgtaaaac
     1441 tggaccttga accaaaatca tcataccact taatactgca taaaatattc ccatctgtgt
     1501 tatcgaccac tttaaaccaa ctactgcatg aattggaaat gaagtataaa agatgttgaa
     1561 tccaagaaaa ataaaaaaat acaagaaaag caaaaacgaa atatgtttta acttgaaaat
     1621 atcgagaaat gtaagttttt taggattgac aattttgtag cattctttgc attcctgtgc
     1681 aaaaaccttt ccaatatttt ctttttctag aacttgtatg acagctcctg ctgccgttga
     1741 tgctgatggt ttggattcct ttaaagtgaa aactattaca attaaggtaa ctaaagagag
     1801 agaaagggct gccaggacag gtaatatttc tttataaata gttgcaccta aaattccagc
     1861 tattgcagga cccacaataa aaccaagatt agaggatatt gccattttac caaagttttt
     1921 gcttctgttg gcatccgttg aaacatctgc aagataagca ttggcaacag aaatattccc
     1981 accagtaatt ccatccaaag ccctggcaag aaataatatt actaatggaa tagttataat
     2041 aaaagttcca ataagagcag aattaatgct aaagagattt tcaacaggta agaataatgc
     2101 acaaaggaaa attatccaac caactaatgt tcctccatga cttaatagaa gaaccttttt
     2161 tcttccgtag atatcagacc accttcctaa aatgggagca ccgattaatt gaaacgcagg
     2221 atacatagca gcgaggatgc cataaactat agcatttcct ccaaaatcta tgactaaaaa
     2281 taccaaaaaa ggcaaaacaa tactaaaacc tagagttcca ataaaattaa tcaataagag
     2341 aggataaagt ggaatctgtt tttcttcact ggtcattatc tatgtaaaat tataatccta
     2401 atttaagaat tttttaataa tttgtaaaaa gctgaattaa agttccaaaa caagtaataa
     2461 tgtcttgaat ttgaaatgat aaggatttaa ttttaggttc tgttttattt ggctctctca
     2521 ttgcaatttc aaatagtttg tattttctat tatgatttta taatcaatat tttttagtca
     2581 accaatgtct ttgtccggat cttttaaatg gttactattg gtcatataag gtaattgaat
     2641 attgtgatag tttactatta tatttattac atagacatcc atagatttaa caatgggtga
     2701 aaatgtcatc ttttcatttc atttttcatt ttctctaaaa gtgagatttc ttacatttct
     2761 ttttgtaacg actatatttg ttatctcaat aacattaaac caagatcaga ttgtaatagc
     2821 acaacaacac caacaatttt tagaagacat atcttttgat atagacgggg ttactttttc
     2881 tcatcatatg gcatccgtta atggaattca aatgcactat gtaattggag gtaaaggaga
     2941 tcctatagtg ttgttgcatg gatttccaca atcatggtat gaatggagac atattatgcc
     3001 atatttggcc aaaaattata ctgttgtagc tcctgatctt cgaggatttg gtgattcttc
     3061 aaaacctgta actggttatg acggtaagac tacggcagaa gatatctatc aattaacatc
     3121 ccaattaggt ttcaaccaaa ttttgttggt agcacatgac gtagggggac aaacagctta
     3181 ttcatatgca gcagcccatc ctaacaacgt gactaaatta gtaataatgg atttcccctt
     3241 tccaggtttt ctacctccag aatttggtga gaatggacct tggtggtttg cttttcagca
     3301 gacacctaac ttaccagaat ctctagtaga aggtaaagaa cgagaatata tatcatggtt
     3361 cttcaaagga ttagcttata atccatctgc aatcacggaa gaagatatag atatatttgc
     3421 aaatcatttt tcatctcctg gtacagcaca tggagcattt gaacatttta gagctttttc
     3481 agttgatgca gaacaaaata aagaatcagc caaaactaag ataacaatgc ctgtattggt
     3541 attaagtggt gacatctatc cagcattagg tggtgatctc cctcaggata tgatgttaag
     3601 ttcaatgcaa gcattagccc aaaacgtaca gggtattaca gttccattgt caggacattg
     3661 gattccagaa gagcgtcccg acttttcaat agatcagcta ttcaaattct ttggaaatag
     3721 tactaataaa taaaaattga atgttctatt ttacaactcc tatagtatcc ttatttttta
     3781 taaattgcat attatgagag aaatacaagg aaaaagaatt ctaaaatgtt agatataaac
     3841 aatgaatgaa ttcttgaaat agactattac tttttgcaat aaaaaataac aaatattgca
     3901 aaggtctata taaccaataa attaaatcga tgtttttttt gtttatacct atatattttt
     3961 aaaaaaatcc aatgccggtt agatataatc gactttgcta atcattttat caattataaa
     4021 aaattaccta attaattatt attcgttgtg gcacgagtcc tccgtcagtc ctctgcattt
     4081 ttttcgtatt ctaaatagca tttattagta caataggttt acgggtctga aatagtaata
     4141 tttttaaaaa aatattgagg aaacaaaaca tagaaattat taacagacac tatgacaaat
     4201 taaatgataa ctgaaaattc caaatactga tataaaatcc tgttttttgt tttcatgtaa
     4261 atgtcgcaaa tgtaaaccaa caacataacc tccagtacaa aatgggtacc agatacagga
     4321 gcagattgaa acggtatgat cattttcaga tattggaccg ggtcttgcca ctcacggaat
     4381 catcaagaaa agtaacaacc tctcactata acaagaatat aaatagaaga ttaataggag
     4441 taccaaaatt tttcaaccat acaaacgtct attgattact ctttctgctt caaggttatc
     4501 gaattgtata accctaactc gaaaaattct atgacgtcaa taaaaaatat tataatttaa
     4561 tattttttgt aaaacctaaa ctttcatttc atatcatatc attacatgtt aatttaagtt
     4621 gattttattt gtctgaaccg atgaaaggta tatgtcatta tattttctag tttaaagatt
     4681 ttaacaaatg tgaaatatag cgtcataatg gcttactttt tttctttgga taatcagctt
     4741 gaccaaggta atcgcttaga gacttaatca tgggacccat cttaaagact ttgggaataa
     4801 catctatatg aacatggttc tcatctagag ctttcattgt agaaggccca actgcaacca
     4861 ctatgacctc cttgttaaga gtgtattgca attgttctct cagtgaatag gcttccgcta
     4921 tcttaaagag gtcatggaca gatggtggac tcgtaaatgt tattgcatct acaagtccct
     4981 tgctgatatc ttctatgagc ttgaccacct ttgcttcatc aggaggtata gacttgaagc
     5041 ccatcctttc aagaattcga gccccactct ccttgagatc aagtgaatat ctgtaggttg
     5101 aggattcgaa gacctcggcc cccactgcat gaagctcatc ctttaggaga tgagagtatg
     5161 acccatgcca gagaacagct acatttcttc cagccatact gaaactcttg agcagcttag
     5221 ctattccttc ggctgtgttt tcctctggta tgatgtctgt cttgattcca tggtttgtca
     5281 aggcaatctt gggcttgaaa cttctagcta tgactgttgt tttctgtaaa gcttctacaa
     5341 acttcttttc tatccctaag tttttagcag cggacataag agaatataca cctggaccag
     5401 tcatgaagac cacgtaatca actctctctt ctaatatctt gtttatgaag aactctgttt
     5461 ccttagatat gtcctggctt gcctctatcc ctactgttgg tgcgagataa ggtctaccac
     5521 caaagctggt aaccaaatgg gctagctctg atgctctacg acttcctgtt atggcaactg
     5581 tcaaacctgc taaggacata atttatcacc ttgtgtagac tctaccatca agaagttttt
     5641 tcttgtagtt ttaattagat ctgtttctat cgtgggaaga aaagccttaa tattttttat
     5701 cttacaaata acgtctaaag aaacaacagc aataatagta acaaattctt ttattcgtat
     5761 atatatattt cctttcgttt gaactgtggg caacactacg gaacaccata atatgtctca
     5821 ttgctgctac tggatggacc tttctcaact gagatactaa tagtacggaa ttatatgata
     5881 tgttatacct ttgttcttca attctcttat tttcaattcg tatacagagt ctctgttttt
     5941 acacagtaat gtctttgaaa ttgtcttgtt aagaacagat attctcagtt aataaattct
     6001 aaaaacaaaa agtatttttt gtcagaaatt gaatcagtca attcttccta gaatgtcata
     6061 atggtattat tatgaatatt gaaatatata ttatttattt ttcctgttaa ttattctact
     6121 actaacaatt gttcccaaaa ttcctgctcc aacagcaggc acagctagta aaggatagac
     6181 ttggacaatc atgtcaaaat atgtaggagc agtaagacta ggccataaag gttgactaga
     6241 taatacttca ttatagatat gggttatcat tggataataa atcataaaaa aggaagagcc
     6301 aagaatccct cctgatgcat aaatgaacac tctctttaat ggtaactttg ataaaaaaat
     6361 atcagccatt ataataggaa tgatgttaag tagatagaat ggaattgtag atatcaacga
     6421 tttatttgga attatcattg taactattgt aataataata aaaattgaac cagtgataga
     6481 tataatacca aactttctct ttttgttacc tatttcttct actaatttga ataatgatac
     6541 taaaataaac gatactaaaa acggtaatgt gagagtagct atagtggctg caatagcagg
     6601 ttctaagtta aagttatagt attctgtatc tgaaaaaggt aatgagaaca tataaattaa
     6661 tccggatgat gacatccaaa ccggtaatat tcctagaata gttaatgttg tatatagtga
     6721 aattgggatt ttttcattct ttttaaaagt gtcattatta ttattctgtc ttttatctgg
     6781 gctaatacta ttgttgatga tgacaataca tgaggttatt cctatcagag ctccaatact
     6841 acctgctaac attccaattg ttaaagtaag atgtggagga cttagcaaac catctaaacc
     6901 gaaagctgaa tgccaagaaa aatcaagtgg tccagctaat atcaacatag aaacaccact
     6961 aattactaat tttattgata aatctagttc aagacttttt tcttcttttc tagtagaaat
     7021 ataaagagaa taagacctcc aatgtagaaa cataattgta gctccaacta gggctagacc
     7081 tattccacta tataatataa catgtggagg agataagaag gtttcaggtt tatttaaaag
     7141 atgatttgtt atatcccaac taccaccact ttcagctagt aaaataccgg aactaactaa
     7201 tacacaacca accaaaaatc tatttattaa tcgcttatag aaatatgaag aggctacaaa
     7261 agtactatca ttattattgc tatactgttg attttcggta tggttatcag aaactcttcg
     7321 tctaaaacaa aatctctttt tcatatcaga tttgataatt aactatactt ttcatcaata
     7381 ttatttatta tttcctattg tttatttatt cactacttct acagaagata cagcaacaaa
     7441 ttccccctga tgatctttaa tacctgatct aggataatga tttaactcat tgatatgcgg
     7501 taatgctatg gtttttacaa atataacaaa tcttccaacc gtactcggtt ctacttctag
     7561 acttatctga tagtagtcac cagtctgcca aggcctacta ttagcttcta tagctggata
     7621 agatgcatta attacattat ctaatcctac gtattctgat cctatttcat cttcaatttc
     7681 aatgaaaata ggtttttgag taaagttata ttttaatatt ttaacgatac tatctatagc
     7741 agtaatatta ggaaaagaca agtaaacaac ttgcatatct gcagtatctc cattgttcat
     7801 tgcagttatt gttaaatcaa atctttctcc aacaacaacc ttcgaggcag acaatttagc
     7861 tccagtaata actggttgag attttacttc ggatgatgat gataagtata tttgtggaat
     7921 aaagaatatt gaaagcgaat agtaaattat taaagaaata gcagtaataa aaatgagtac
     7981 gatcgtaata tttactcttt ttatgctatt atgtctttca gaatagtcaa agttcatgtt
     8041 taatcaatca ataacaaaat tcatagtgaa tgccttctaa atatcctttc ttttgagcat
     8101 gtatatcctt aatttttttt ctgtaacata attttgagct tgtcttataa tcacttttat
     8161 atataggaat atttggtaaa tttgtcacag tatataaaat aatagtcttt gagtttaaaa
     8221 acacaactat aattttaaga atttctattt caataaaaat ttaatttatt tgtatctatt
     8281 ataattatga gttatactat atttatttag gtaacaatat ttaattgtgc tgtcaaatta
     8341 cataagagtt aagaaattat tatttcaaat atatcaatag aatgtctcaa taatatattt
     8401 cattgttatg caataaatca tataaacctg ttataacata tattaagaaa ataacaaatt
     8461 atggtattaa attctataat aattataggt agttattata tatattacgt aattattata
     8521 tataaaagtg caaataaata ataaaaaaat tacattatct atattgtttt atatgttaat
     8581 tatcgttatg acaataacta ttattgatta taatcgttat attactcact tatctatatt
     8641 atatgttcac gctgaaccta acgacaacaa caaagaacaa ggagatagtg aacagaaaca
     8701 agacaaaaaa atagaaaaaa aagaagagca agaccaaaca aaagataaag ataaagatga
     8761 agataaggat gaagaaaata gtaacaaaaa ttacaattca gattctgatc aaatggatcc
     8821 tgcatataga cctttctttc ctataaagcg aatagtaata cctgatgttt atgaaactat
     8881 accaaacgga cagacatata gattcaatat taataatcca aatgataaag tccaagtaga
     8941 taaaacagat aaatcaaaca tctttacaaa acaaaatctt gatggaagtt ggagaataga
     9001 ttatggaaaa ccaagaatag atatccatac caaggatgct ggaatattac ctgataaagt
     9061 ggaccaatta aataatttgt ctaaaggtaa aattcaaagt tggaattatt cagaattaaa
     9121 agacatagga tattggtata aaccaacaga ttggaaaaat atagaagtta ctttggtttt
     9181 taaacttctt gattcagcac gttctaaagg agatcaacat gctctttcaa tagtaactag
     9241 gtcaatatca catagtcaac ttgatagtga gtataaaaag tctgatgatg aaccacaatt
     9301 tttttgtggt ggatcaagtt accacaataa cctttccaat gacggaaacg tgagaatgaa
     9361 aaaagaacaa tttcatattg attatgagtg ggaaagatac aatcccgaaa tacaagtagg
     9421 agatatatat aataaaataa ttggattcaa agcaatagta tacaatgtta atgatactgc
     9481 agtaaagcta gaaacttggg tagatacaca aaatcagggg aaaggtccat ataaaaaagt
     9541 acacgaagga atagatctgg gcaattgggg tgataatatg aaagtatgtg gtgctgagac
     9601 agatggtcaa gcaattacat ggggttctcc aattgttata ctcaaagcaa atgattttaa
     9661 atttgacata tatgatattg aagtaagaga aattattcct cctcttaaac aaatttcgta
     9721 gttttcttga tttggttaga ctagaatcag gaagaaggtc aaaatcagtt tctaaaatta
     9781 aaatacctga acctgatttt taattttatt tttaattctc tcagatttgt tgaatgaatg
     9841 attgtaaagt ttatggaaaa aatatactta ttattttaga attattatta ctaaatctat
     9901 aaatacacaa attcaatttg atgattaact atgagtctaa ataaagctta cgttagacat
     9961 ctatatctgt ttattgttat ctctatagta tctactatag tgttcgtatc ttcctatgat
    10021 ttatcttttt cccaaaaaga tgccaagaat aaagaagata aagatgaagg taaggataaa
    10081 ctggtagtta aagctcaaat aaatttgaaa aatattgatt tagaaaaaac gaaatttata
    10141 cgtgttattg gttttataaa tggtgaagag agtaaaaaag atattcctat ttcttcaata
    10201 gacaagtctg agaataatct agatgttgaa ttaaaagtga atgaagaaaa tgatatagtt
    10261 gaagcaagta caccagatga attctttgta tgtgcatatc aagtaggaga tgtacaaaaa
    10321 caggaagcta ctactcctat aacaaaattt gattgtaatg aaggcgatat attaggcaat
    10381 cctactgaaa ttagtttatt tcaatctggt tctcaagtat actctaaaag ccaagtatat
    10441 taccaagcac atctaaatac aatcaatatt acaaatagtg atactgttaa aataaaaata
    10501 cttgctcctt tggcagataa aaaagatacc gaaaaattaa taattgctgt tatgataaaa
    10561 ggtcaaataa aatctgaagt tatagaagat gtacaaggag aacttgacaa aagtaaaggc
    10621 actacaatac aaagaacatt tacatttgat agagatacag acattggcaa aatacaaatt
    10681 ggagatagat atcatgcatg tgttgcaagt aatgatttga gtccaccaga aggccaagaa
    10741 tgcgaaaaga gattagtaaa gaattttgat aaatccaatt ccttacccgc ccgataaata
    10801 tttaggcagg gtttgatttg aataaaatat agttatttaa tctctatcgc taatatacca
    10861 cctaatgatt tgatacattt taattattta gttaaaacta taccccagtt tttgactcct
    10921 tattagaagc aagtaatagc aggaggtgag gctattacac actttaagac ttttccttgg
    10981 cttttactag aaattatatt tatcaacagt attctagaaa tattgagact ttaactattc
    11041 atgtgctttt catttgagtt ataatttcta ttgaagtgtt ttataccatg atgttctatg
    11101 acatttatgt cttttcaaga agaggaaaaa gaaattactg atctaagacg aaaagaacag
    11161 ggaaagacaa atttatcctc tattgatgtt cacatagatg attattatga aaatgtaatt
    11221 tcctcttcta atatatttcc tcaaaaaaat aaggttccac cattgtattt cgatgattta
    11281 attaataaca atctaacaat taaaaccctg aaagacataa agtacaattt tattgaagaa
    11341 cataggaata tcctaaatac atattattct atttattctc aacgtttgga tgatataatc
    11401 tattataatt tggataactt aatggcttta gacaatcatt ttgataataa taatattaac
    11461 caaaacctaa ttaataatca aactaatact acaaaaataa taaatgacat tgtagataaa
    11521 aatatggata catttatcaa atcaataaaa tttgcaagta aattttactc tgatgttact
    11581 aagagttatt gtaactatat aggaatgatc ataaagtcat ctgaaagata atgtctatta
    11641 gtctacacta tcagctattc ctgcgatctc atttgtaaca ggaatggtag ccagcatgaa
    11701 cgagcagaca attgcaatga caattctgag tattgataat ttatagaata tggatattag
    11761 aagagatcta atttacatta gtttaggaaa tttcttaaat tcattgagtg aatatattat
    11821 aacataaaga aatagaatat cagaaattta taatagaaat caaaagtata atctaatttc
    11881 tcgaaataat cttataactt aaaaagaatt ctctcaacaa ttcttttata tgataatatt
    11941 ttaacgtgtt tattgtcaac aacggttacc aataaaaaat ttgaatatcc tggtagtaaa
    12001 aggaactttt ctccattgta cagattcaca ataaatcata tgtacctcaa gataaaacca
    12061 gattttaaaa aaaaacaact tttagattgt atacaaaaat taactattga ggcattatat
    12121 aatattgagg aacttgtact agatattgca gaattagata ttcataatgt ttcttcagaa
    12181 actgataata ttgatatcaa gtcttttgat attttgtata aaaaagataa attagttatt
    12241 aaatttagaa atatgataac aaatggatct aaatttatta tcaaaataaa ttattcttct
    12301 ggttattatt ctaaaggtaa acctaatggt aaaccaagaa gtggatttac ttttattgcc
    12361 caagactatt catctaattc tactgatgct atccaatcct ggactcaagg tgaaacggta
    12421 gagtcaagat actggtttcc ttgtattgat aatccccaaa tgaaatttgc aagagaaata
    12481 gtggtagttg ctccgactga agaatatgat gtaatttcta atggcgaata cgaaagaaaa
    12541 gggaatgtat ggttatggaa agaatcgacc ccaactccag cttatcttac atcagtagta
    12601 ataggaaaat tcaatacaga tggttttgaa aaaaatattc ctcctctaaa gtactattgg
    12661 cctaatgata tttctaaaat agatgcgatg agaacatttg ctgatactcc taagattata
    12721 aaattctttg aaatttattt agacacaaaa tatccttata gtaaatactg gcaagtcata
    12781 gtcgacaaat ttgaatacgg aggaatggaa aatacaagct gtactacact aacacgaaat
    12841 atcttgcatg atgagagagc atcattagat tattctcgag atatcgtcat agtagctcat
    12901 gaattggcac atcaatggtt tggagattta attacatgta aagattggtc tgatatatgg
    12961 ctaaatgaag gatttgctga ttattttgaa tctctttatt ggggtcatag acatacaaaa
    13021 aaatatgaga agaaaaaaac aaatgatgaa ttcttgtatc aaataattca aaaaattgat
    13081 acatatatgg aagaagctag tactttatac aaaagaccaa tagttaccaa tttgtataaa
    13141 catccagatg agttatttga tgctcattct tatgagaaag ggggactagt tcttcatatg
    13201 ctaaaaaatt ttataggaga agaaaatttt aaaaaatcta taaaaaaata tataaataca
    13261 tatagagaaa gaacagccaa aactgaagat tttcaggaaa tttgtgaaaa aatttctgga
    13321 atggatttga atcagttttt ttatcaatgg ttatttacag caggtcatcc cgaacttgat
    13381 atacaaatta tggaggagaa gaataaaaat accagaatga aaaaattaaa agtaaaaatt
    13441 actcaaaacc aatcaggtga ttttattttc gtctttcctc tagaaatacg attagtatat
    13501 cgtactaaca caaatgagaa aagcagtata gataaaatag aaagaataca agttaatgag
    13561 aaagaaacaa aatatacatt taaagaagaa atatctgcta atgcaataat tgatttaatt
    13621 tctattgacc ctgaattaaa aattctcaaa gaaataagat ctttaacaat agaaaaacaa
    13681 aatgaaaagt tcaatttact tcatattttg aaaaatcaat taaaagacca aactacaaca
    13741 attgcagaga gaatttcagc tttaaaaata ataaaagaaa acttttattc tcaagaaata
    13801 ttggaattgc ttaaagatat tgtaatgaaa gattctgttt tttatgctgt atctgttgaa
    13861 gcagcgaatg taattggttc ctatcacgat attaaagatt ttaataagga taaagaatct
    13921 tatgagattt tgaaagaatg tctcactaat acaatatttt ttacgctagc cccccaaacc
    13981 aaaagagcaa taataagcaa tataggaaaa tttgaaagag aagatacatt agaactaaaa
    14041 aatgaaaata atgtaccatt acttgtagct ctattagatg atcaaagtta ttttgtagag
    14101 aatgcaacag caactacaat aggtaaatct atcaaaaatt taccagatgt aaatccacta
    14161 aaagaagaaa tgataaagat attgaaagat aaagttaatt cgattacatt tcaagatcaa
    14221 ttagctcaag gcgcaattaa tggtttaagc gaacttgctc aagatgaaaa tatagaaaaa
    14281 attaaagata ttgtaaattt attaataaat aaaagcctcg aaagagattc tgtcactgaa
    14341 actatgaata ggtattttgt aagatcagct gcaactcttg ctttaggtaa atttttagtt
    14401 actaaaaatg aaaaaataat agatgataaa aatcttaaag aacgagcaga taccttaaat
    14461 aataaaattt taactcatct tatccaatta ttaaaggatg gtgaaatgcg cagaattaaa
    14521 agaaatgctt gtacggcttt agctgactct gatgctaaga ttgtacaacc taatgaaaga
    14581 gtaagaaaaa gtattgatgc actaaaaaaa cttgcagaag aagatgtaga tggatttgta
    14641 agaagaacag cggaagtaag tctaaatgta ataagagaat ggctaaaaga atggaccgat
    14701 acacaaccta aattggacat taatctgcga aaagatattg atgataaaat tgatgcgaaa
    14761 aaaataggta aaagagagaa gcatacagac ccgggaaata atgatgaaaa aattctcaaa
    14821 gtatctagaa aagaagtatt agaatattaa ttgtagaaga gttttaataa atttgcattt
    14881 ttagagaaat gtcttattaa agctgaaaat aaaattatcc aagacgtatg atttaggacc
    14941 gaccaatctt gggtttagca tgagatatga gcgaaatttg atctacaaga gggattggaa
    15001 ctgatggata catcttattg aaaattaaat tcttattgtc tggcagaaat aattcaccga
    15061 ggatacactt tgctcttcta cctccataat tgttctcttt aattatttct aaaaaatcta
    15121 ttttagggaa ccaataaagt tctattatta ggcctcacca aacacaatat ctggaggcaa
    15181 attatgctca atcacagata agattatatg aaatgattat tgaaattttt aagattagat
    15241 taatgttaaa gattttgaat cttttatttg ggattattac gatcagtatt atcttatttt
    15301 ctgtaaacct atctgtctaa gtttatgctc aagataatca atactattat aacacaaacg
    15361 aaaataattc ctcactttta aaagaactca aaatcataaa acaatacttg aaaccaaagt
    15421 aacaaaacta gctacagctt tacaaattgt tagttattta ccgcaattac aacctcctga
    15481 tataaatttg atagatccaa acattaatgg tataacagaa gatgccgata tagataatag
    15541 aaaaatagct caaatactct tggatcaatt taacgaattt tcatccattg tatttttatt
    15601 aaataatggc gatgtatatt ttgatgaacc atttgaacgt caaataaatc taacgacaga
    15661 caatctttca tttagagatt attatcaagc tagtgaaaca gactaaaaaa acttaagcga
    15721 tgcaattatc tctaaggcaa caggaagaaa tcttgcagta attgcaactc cagtaatcaa
    15781 aggtaatgat agataggaat actgtatgga actattaatt ttaacaatta tgaccagttc
    15841 ctgcaatcgt ttaatttaca aaataatacc agattggtaa tgattgacaa gaatggagta
    15901 aaaattggta aatctaatga aaatgagact tcaatttcaa aagaatcctt tgacacaaaa
    15961 gaacagtttt caaatcttac ctcagttaag tttgaattgg aaggtaagtt aggtagtatt
    16021 ttagaaaaat ttgatgggaa agaaagtcaa atcacatttc ttccttatga gatttttcaa
    16081 aataaaagaa tattactact tattcaaagt tgcaacagcg atggaaatga atcaaataaa
    16141 tgcatagaga ataaagacca taacctagtt aacgagaatg ttttaagtaa attaagcagt
    16201 ttgttttaga ataacttact taaaaattgc cattatccaa taaaacaata tcatctgtat
    16261 gatattatct ttatttcttt tttttgaatt tttgaatata taattaaaag ttctatcaat
    16321 ttgctaaaat atagagctgc acatcttcta gttctattct taagttatgg tactacattt
    16381 gaaaaagaaa aaactaattt tttttgtttt cattattaaa gaaaaataaa taagttatat
    16441 tgtctaaatt tatatgctta tcagaagggt tggctaactg attattatta ttgcttcgaa
    16501 aaaaatcttt taggcttaat aaaagagagt ttatgttcag aaagaagaat attccaatct
    16561 ataagcaaca taaataaagt catcagtata cctacaatat tcataagtat taaagtttga
    16621 atgtcagagt ttagcatttc tttaaatgag ttaaagaaat aataagatac atatatcatc
    16681 attatataga caagaaatcg tcctaaccaa aaccctacta taatacttat ggattttgca
    16741 ttaatcagtc cataagaaat aaaaagcatg ttactaggta atggaagaag tgcaaataat
    16801 agagttccaa tgaaatatcc ataccgagtt ttttctaaat actttcttaa cttttctaaa
    16861 ctactttttc tttgatcgtt tattgttttt cttccaaatg tacttaattg taatagtaat
    16921 aaacgaccgg caattgcagc agtagctcct attatcgata ataaaagggg atttagttgg
    16981 tcatttaatg agtatgctga aacaactact atccatgttg gaggagttag tatcggaata
    17041 acgcttaata caaatattat aaaaaaaaca aatggatatg tatattctga aaataaagta
    17101 ttgaaaaaat catataataa taattccaaa tatagttctc aaatattttg tatggtttag
    17161 gtattttatt tttatcattt ttcgtattaa taaatactga gctaaactct aattcaacaa
    17221 gagtatccta agaatatatt tttgaaaatt taaaaaggct ctttcttaaa tgaccttaaa
    17281 tataccaatt taatagcgtt tgaataaaac tcattattgc cgaaaagaaa ttatactctt
    17341 gtgtaatggg agaacaatga aatttaaaca ctccgatgga ttcattataa tgaatacaaa
    17401 aaaagggaaa ttcttacttt ctattattat tgtttcatga attctaaaat agataggatt
    17461 ggaactcaga gttcaatggg gccagtggtt tccgtggtga tattggttct aaaaatgact
    17521 caagaagtta ttaacatcaa tacttaataa agaattttat ttgtaattca attctaataa
    17581 ctctatccaa agtgtaagat atatcaattc tttattaata atttattaat tctattaaaa
    17641 ctagttttga atatatttta tatctcagtt agagtgattt taatttcttc atttactttt
    17701 tcataatcct catgtcgaaa atattgttct attggaagaa aatttttatg gaaattttca
    17761 aagtatgttt aaattatttc agtggcatgt ggatctacta aaattattct cttattagaa
    17821 tcattcaaaa attttttata aaaaattttt tgatgataga tcgaaaaaaa ataaaagtat
    17881 tggattagtt agattttaat ttgaaaaatt aagtttaaaa agaatggtgt tatatttttg
    17941 tcctagttct agagttaaat atatataaat aaaaataaaa atatccatat caaaattttt
    18001 tacaaggaat tatctaaatg atagagagaa cttttttatt tattctctat tacttttaca
    18061 attttattgt aaagttctac aatatggtta gaacaggtat aatgtatttt gatagtacca
    18121 tctttatgaa attcatttac acttattgtt gttgctttca ttgctgccat cttcgcaccg
    18181 catacttcac aacttaatga ctttttttct tctagtttat acctcaacat ttcttctagt
    18241 taattattat agtataaact tctttaaata tgtatactca aatcaatttt ttatatactt
    18301 tataatgtaa ttataatcac catacatagc aatagtaata atgtagtagc aattactctt
    18361 ttgaatattg tttatagtat aaatttgcaa taatatcttc tttcattatg tttttgttaa
    18421 aagttttaga atgcaactag tataactaaa ttaatttttt atttaaaaag attatgtcgt
    18481 gttcagttta aaatagtcta ttacttcgcc attgttggta tgaaattcac ccattaatgt
    18541 tttgccatta ttttctattt ttacatttaa aaatccaaat tgactttcct gtaacacata
    18601 aaaaccatat ctttctttaa caggatctaa tttatctcct gcagttccta ctgtcaaaaa
    18661 tactattcca ttatctttat ctgagtattc tgatttcaca gtatttgtaa ttattggttt
    18721 tggttctatt tttttatttg ttgctttttc atattgttca ttatatagta gtggataggt
    18781 tctttcatag tattggttgt gactagatat taccaagtct acatcatatt gttgaaagag
    18841 ttgttgatat atatctcgca gttgttctgc ttctccttta tcttgacttg tagaatataa
    18901 tggcttgtgt tgatgtacaa ctatccaatc tacactggaa tttttagata atttctctaa
    18961 atcatttttg ataaattcat actgtttact accctgttca aaaggatgct cagtagacat
    19021 actaataaag tgaatatttc taaagtcatg tgaataatat ggactcgtta gtttgtggta
    19081 ggtgacaatc tgtttgtaaa tatttgcaaa ttcagcatca tggtttccta ttgcaatttt
    19141 cattttatct ttgataggtt ctgacatttg aatccaacaa gcagcagatg gagattcctt
    19201 tacctggtct ccggttgtaa ttattaattc aggatttata gcgagaatat tatttattgt
    19261 cttttttgtt tcttcgttac agtaccaatc tcctactgca ataaagttat aatttgttga
    19321 attattatta ttatctggtt tctctacaaa gttattaata atggtattat tttttttagt
    19381 aatatcagca tcttttattt tatttattat actttcataa cgttttttat tttcactcat
    19441 tttattattt aaattgtcat tagttgtttc ttcttcttct tcatattcta tatattgtcc
    19501 aggatcaaaa atataatcga attcctcatc attgttgtca ttctttatcg catatgaaga
    19561 tttaaacaat gatagagaga agaatagaat acatgacaat acgaatacca taattactgt
    19621 attgctatca attttacttt tcagtaaatt catctcctta atttatttgt tataacattt
    19681 gaataattgg acataattta tttaacctta tcattaaaat tattctttaa ttttttattt
    19741 ccaaattatt aatttgtaat tttttcctct tgtaataaat tttcatatct tgtcatcatt
    19801 aaatgaagat acaatatagt tagtatattt ataatactat tgtattcata aatgatgata
    19861 ttccctacaa catcatcata ttttattttc ttatacataa gatttttaat ttattatgta
    19921 atatttaaat agaatgatat tacattactg tttttggtaa gataaaaaat aatagtgtca
    19981 tattctagtt taactatcga ataataaagg attcttaaag ttaatagtac aatggtctaa
    20041 cttgatacaa atagaatata aaagaaatag aattattgtt taaataattg ttctgccttg
    20101 tctaaatttg ttttaatatt attatgtaaa gtagtgattt cttcaatagg ttttttgtct
    20161 tcaatagcac ttgcaaaatc ttctcttatc attacttctg tttcttccat tagatcatta
    20221 tcaagttcct ttaaaggatc ttcaatatac tcataattgt cgatatatgc aatatctacc
    20281 aattcagatg cgccggtata atttcctgca tttaattctg ttattgattt ttgtaacaaa
    20341 ttcttgatgc ttgttaaaat aattaatgga tcattttctt cattttcttt ttgtgcctga
    20401 atatgatatg agggggtaat tgcaacaact actactaaaa ttatagaact gatagtataa
    20461 aatacttgtt ttgaactcat caatattttc ttactaaaat ataaatatat aaatcattaa
    20521 ttgtttctca ctttagagaa tcatattttc cggtaatgta aaacatatta tatattaata
    20581 atttagtaat ttgactattg actatgccta tttctcaaga atcatcttta gaattttagt
    20641 aacatcttgt gaaatcttta agataacaat ttataattga aaaatgtaaa tatcagacca
    20701 gaataattat atatactctc tctagagaat tattgtttta attatctagt aaaaactatc
    20761 aaaattgaat attataaaaa agtttaataa aaagattcaa tataaaatat tacttatgat
    20821 taattttata cgctatcttg aagtaaaatg gaaaaaaaga ggcagcaata tttcaatttc
    20881 tactaattga ttttttgaag ccattttaat tgttgtgggt attatgacta atttactatc
    20941 tcgaacttcg tagtagtagt agtttatttt ataaactaag atttttttac tttaaaatta
    21001 tccagtatct tatcattatt tgtatgaaat tctccaatta ttgtctttcc actgttttct
    21061 agtttcagat taagaaatcc atatttccct ttttgtatta tataataagc atctttcttc
    21121 ttaatctgtt gtagttcatc tcctgccgtg cccacagtta aatatatgat tccatttgta
    21181 ttttcgtaaa tagatttcga atcatcatca tcatcttttg taagttcagg cattgattct
    21241 tggtcttttg tttcaagtcc tattacttta ttgtatgaca ttggatatga cctctcatag
    21301 tattggttat gtcccgatat aacaaaatct acaccatact tttcaaataa aggtaaaaaa
    21361 gtatccttaa tacgctctga ttcttcaata tcagcatttg ttgaatataa tggtttatgt
    21421 tgatgaatta ttatccaatc aatactagaa tttgtggatg atatcttcaa atcattttta
    21481 ataaatttat attgtttgct gccttcttcg taaggatgtt ctgttgataa actgatgaaa
    21541 tgaatatttt taaagtcatg tgagtagtat ggacttttca aattgtgatt tttaattatt
    21601 tgtttatata ttttagaaaa ttcagcatca tgatttccta ttgcaatttt cattttatct
    21661 ttgataggtt cggacatttg aatccaacat ttagcagatt tttcatcttt aacatggtct
    21721 ccagttgtaa ttatcaattc aggatcaaca gaaataatat tttcgatagt gtcttctgtt
    21781 tcatcattac aataataatc cccagcagaa actatattga tatcttcatg ttcactaaaa
    21841 tcagaatctg acttttgacc aaatattgtt agattattaa tatttaatga aaaaaaaaat
    21901 aatactaata atgagagaaa tgaaatttgg taaatataat ttgaaattct atatgtataa
    21961 catgtaaatt cattattatt gtttaacaca tttgttcgga attatctttt tttcttgaca
    22021 tttaataaat aatgatattg tttctccaat gtgagaataa gatatagtat taattgttaa
    22081 tttatctatt atatgatgat ctttctatat ttcttgccta aattgaaatt attttgtatg
    22141 atagtagtgt tgtaatatct ttgtgtgagt tctaaggatc tttatattca tatctttatt
    22201 cggtctcgtc gaaaatattc tgatagtgaa gcaaacattc tagcaaaatc gcgaatatct
    22261 actttcatat ccattgaatc atctgtaaaa ctattatgta aatgtaaatg gaattctcct
    22321 tctctttcta tcaataacat ttcaatatca ttttcaaaat cgccacattc aaaaacaata
    22381 ttgggaattt gtggtttttg ttctgttgaa attttatgaa tcataatcta ctatatacta
    22441 cattcatcca tatattcgtc tatttctgag gtttatagaa agtccagatt atgatggcgg
    22501 cttattgtta agaacattga tgaaaataaa aattattcta gatacattat taatgcaata
    22561 tgactatcat taggagaaga atttattctt tctaatgtat taagttttat atctataaaa
    22621 ttgtctacat caattttttg caagaattga tttatttgag attctaatga aattgctgaa
    22681 ccttgtgtct ctaagatttt tacttttggt ttcatttcag tcaataagga taaatattct
    22741 attaaaatat tagggtgatt ctcaaattaa gaataattaa tcctcaaaat tatcaaaatc
    22801 tagaaagaga aattaaataa taaatatgta aatattattt ctaataatta ttatttattt
    22861 gaatgtattc aaaggtgcca tctatataga catacaatac tcattatccc tagtccactt
    22921 ctttatgtat tccgactatg aaatttgtat gtctatattt tttttgtatg caaatagtcc
    22981 tttatataat ctaaatgttt caagttagaa tgtacactta aagtaatcag ttgaaaagag
    23041 tatatccagc ataccattat atcatcttta agcatagttt actttaataa ttaattaatc
    23101 gacttttttt acaaaaagta aagaaaaatt ttcttagtta aataaaagtt atagtaatta
    23161 aaaatcctta cttattagat tcatttacaa ggtcaatatc tagtttacgt tgaaaaaatt
    23221 tccattctcc attgttattt ttctttatta tatcactata agtacctgag gctactactt
    23281 ctggactgtt atgtgcttct atcactgtta gatatgaatt tgcactagcc atattatttt
    23341 gtgcatcttc aattataatg tttgttgata aatgtctttt accactagcg aattcatttg
    23401 taacttggtc aatcatattt tttagttctg ttttaccttt tgcttctcca aatgttgtag
    23461 tccatattcc atcctctgtc caagtgttaa tccattcatt taaattttta ttatctatag
    23521 caaggttata tctagcaatt aattcttgaa tggttattct atcgccagta tttatcatga
    23581 ttaaacgtaa taatattatt actattattt aactccaatt cgttattttc aatttagaaa
    23641 ataaatttta atttatcata attaatataa taagtttcaa tatagaacat atttatttat
    23701 tttaaacttc ttgaggcata ttatcctttt atgcctcaag aagggggtgg ggggttgaat
    23761 gacttgttca tgccaatgca tattccagat attttttatt agggtgacct aatatttaac
    23821 tatatagtta attcttaaag actagaaagt aacatggtat tattataata attttcagat
    23881 tccgtagcga gaatttcagg aattattaaa tacggttatc tcaattagta atttcactat
    23941 taaatcctta aacaacgatg aaataggagg catatatatg tcatagtcta gactttatat
    24001 catcttcgcg atcatctagt ctagactatg acaaaattta tttgagtaag aaaaagtatt
    24061 tgtattgtat ataacttgtt gtattagtat atatatttaa taataaataa ctatatgata
    24121 taataaaacc ttgatccaat actaaaacaa caaatacgtc aaaatacgag aaaataagta
    24181 taaacttcat attacataaa ctatgaaaaa aagaaaatta aataaataaa tcaagaatac
    24241 aataacaaga aataatacta atccattatt ataaatatat gataaaaata aaacaaaaat
    24301 aacatttaac tatttaaata atttataata acatattcaa agcatagaat gagaatttca
    24361 agaattatta aatatattga tcaataactt attcttgatc tttcctatca agatctatta
    24421 gacatgtcat aagtcagtat catagtctgg actaaataat caagacacca aagtcataag
    24481 ttcagactat gactataaaa ttgtatcaat ttgattttat ttaaattgtc atacacggaa
    24541 ctagatattg gttatataca gatccacaat atgaagatga tagtattgac tgtatgattt
    24601 atttatttta agataaaata tttaccgatc aaggaattct tgttttgaga aatgtactat
    24661 aataattata tagaacaaga tacagtcata gttaatgatg ttatctgcaa tggctaaaaa
    24721 aaattcaatt gcaaaagcat taaaatttgc tcttagtgga ttcagtctat tttttattgg
    24781 catgttattt tttattgtag tatatgatgc gttaacaggt gcatcttccg aaacaacaac
    24841 agaatattgt gctaaatatg gctttttagc atcgcctaag tgttggtaaa aaaataattc
    24901 aatgggtgtt aatgtcatcc tctaacaccc atgtaattga agctcaagac aatgcttgaa
    24961 cgtgtataat aagagaatat gaaataaaaa ctgtttattt tagaaaaatt aagttatgta
    25021 acttatctaa gagaaccaat aatagaataa tatttaacaa atcaagactt tatttttcaa
    25081 atataaaatt gaagcaaaaa ccttgataaa atattagtat tatataataa aatagtatga
    25141 aagttatata gaaaactgag taaaatcttt aaaatcattt cttttaagta tcaaggtcta
    25201 ctaatgatgc gaacttttgt agaatggatc ttggatagca ttccacatga attccaaaag
    25261 aaaggagata tagaatgcta tgagaaagga tggaactaca tcctaactat cccaagatat
    25321 ggtataatag acggttaaga tataaacaaa aggctaaaca atattttaaa agattgaacc
    25381 ttttttgagg ccagtggttt tcactgtgat attgatccta aaaatggatt caataaaata
    25441 taaatacatt aaagctataa atgttactcc tctttaaaat ttattgacat tagcaaaaga
    25501 ttttaggtca caatattatt tgtccaattc catatctaaa actaaaaatt ttgatattgc
    25561 ttacaatatt ataactcttg tataaataaa tcttttgtag aatcctgaca tcttgacaac
    25621 taattaacta tttgaaattt tgattaaagc taaaccaata taagaatgca aaagagagat
    25681 tattgaatga taaatgctat tgaatatcct cctggaccaa aagatttact tccatattca
    25741 cttgttagaa aatttctacg agatcctctt aaaatgttaa ctactatatc tcaaacttat
    25801 ggtgatatat ctcactttaa gtttggtaaa cagaatgtct atttcttgaa tgatcctaat
    25861 tatatagaag aagtattagt aattcattac aagaatttta tcaaaagtag agggttacag
    25921 gtatcaaaac gtcttttggg gtctggttta cttactagtg aaggtgatta tcatgataaa
    25981 caacgacgct taatacaacc aacattttat cccaaaagaa tcaattcata tgcggaagtg
    26041 atgataaaaa aaactttaaa tctatgtgat aactggaagg agggatccgt attagatata
    26101 cataaagaga tgacacgtct taccttggat attattagta aatctgtatt aggttatgat
    26161 attaaaccag aagatgatga aattggagat tcactactaa catgtatgga atatttcaat
    26221 cgccttttaa tgccgtttgg tgaattgatt gaaaaaatac caatcttacc aattaacaag
    26281 ggatttcaaa aagcaaaaag aaatctagat tcaatagtat ttagaatgat caacgaacat
    26341 aggatgaaac tagaaaaaaa tgagaaaaaa gataatgatt tattatttac tcttttacag
    26401 gcacaagatg atagtgcaga taccggaaag atgagtgacc aacaattaag agatgaagta
    26461 atgacaatat ttttggcagg acatgaaaca acctcaaatg cacttacttg gacattttat
    26521 ttgatatcag aacaccctga aattgatgaa agacttcaaa aagagattta ttcaattgta
    26581 ggtaacaaaa cggatttatc agttgatgat atttcaaaat tggaatatac tacaaaagta
    26641 ttgacagaat ctatgagact ttatccacct gcatgggctt taggacgtca agcaattaat
    26701 gattgcaaaa ttggaaaata cattattccc tctgggtcaa taattttaat gagtcagtat
    26761 gtaatgcatc gtaatccact ttattttcca gagcctgata cttttcatcc tgatcgttgg
    26821 actgatgaat ttaagaaaaa cctaccacga tttagctatt ttccttttgg tggtggaatt
    26881 agaagttgtg ttggagaacc attcgcatgg atggaatcaa ttctcattac agctatcata
    26941 aacaaactat ggaagatgaa acgtatacca tcaaataaag ttgttttgaa accattgata
    27001 acattgcgtt caaagtacgg aatgcatatg aaaataacga gaagaaacta ctaaagaaat
    27061 cttgataata atgccttgta atttgtaatc taattacatg tacaaataaa ggataagaat
    27121 agaaacttca atacttttct attattttca ttttgtttag attaaacata tgttatctat
    27181 aaattgagat aaaagtttta ttgcaagatc ctagattacc ttaacaatta tcatagctaa
    27241 atgagagtta tgacaaatag attttaaaca attattaaat agttggagaa atgtatatta
    27301 gaaaagtgta tattagaaaa gtcaattctt ttttcattaa tttcgccact ttcaaaatcg
    27361 taccattact atgaatatat tgaattaaaa ttaaattttt ccttaatata ttagagatgt
    27421 agttgggcta tcagtaacca aaaaggaggt tcattgagac tcatgtgtca catgtcctct
    27481 agtttggttt ttcagtccaa agaactccaa acagaacccc ttcatatata gttagacaat
    27541 atagttaaat aaagttatat ccaaattttt tatatgttgc ctttctttaa taagttatta
    27601 cttttataat agatgaatat ttaaaataat aaaaaatatg taaactgtgt ttttgctaac
    27661 taatttatct actttgttgc ttgtcctatg ctatgattag gaattagaag aacttgctgt
    27721 tgttgttgtt ttgttgttgt tgttgttatg aatattatgg cttgaaattt gttggaatgt
    27781 ttttacagta ttagctccta ttgtagataa ttcctttaca ttctcttttg tatgttgcac
    27841 agatgtattg acagaatcta aatttgcaaa tacagtatta tttaatactc tagttgctgc
    27901 tacggcatta tctgcaaagc tactgactat ctttccatag ttttcagcca cttgtcttgg
    27961 agatgacatc cagtctgacc aaagttgtac ggaattaact ataatgtcct tttgagattc
    28021 tacataatta tctgtaattt ctctaatagc ttgtattgta ttttcctgat aatcgttaac
    28081 gatttgtgta taacgtggaa tttcttttct tgcttcatta gttgttttct tgatatt
//
LOCUS       DASVEV010000214         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1257933, whole genome shotgun sequence.
ACCESSION   DASVEV010000214 DASVEV010000000
VERSION     DASVEV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257933"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /gene="rqcH"
                     /locus_tag="VF222_09665"
     CDS             <1..1836
                     /gene="rqcH"
                     /locus_tag="VF222_09665"
                     /inference="COORDINATES: protein motif:HMM:NF041120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HEX7142731.1"
                     /translation="STKGMWVTKMLFKQVEENDLVRNARKELERSRIISIQQLDSERI
                     AILKFEHYDSKIRYLICEFFGQGNIIICDESMKIISILNPLEVRHRTLRIGLKYASPP
                     SYGSDIFKISIEEFNSKFQNETKNLEITKWLGRILSLPKKFVEEILFRAKIYDNRRKI
                     KEITNIELGVIFTNTKKLVDEVICENKHSPVVILDADNVPIDASHVVLKNMEDSTMKP
                     SPTFMDALDEVLCNDIITIGKKIHTVELDKKISVLEHDLDEQDKAKLKVLSKSNSIRD
                     LASDLMNISISDPIALEGLLNRHSAKLIMEKGKKYLDIVEEQIPLDQNLRKVSSLLYA
                     RAKEMERGSETIDNARLKILDEIKELRKKTTIVENKIKLKEQKTKEWFERYRWFVTSN
                     GLLVIGGRDASSNSAIIRKHMTDQDLVFHAEIHGSPFFLIKNIGLDSVDDDTTTTTNN
                     QSIIETAIATVTFSRGWKEGLSSADAYWVNPNQVKKGAPTGQFLPKGSFVIEGKRNFV
                     KALELKLAIGVTKDNDSYKVICGPVDSIKSKSIIYSVIVPGGIDPMNATKKIKTELIT
                     NSHNIDDEDLIEFLKRMSLDDIIRILPSGKIKIVSSEKGALYGNL"
     gene            1926..>2309
                     /locus_tag="VF222_09670"
     CDS             1926..>2309
                     /locus_tag="VF222_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7142732.1"
                     /translation="MTTRVLVRDVMNSPIISSSPDSNIKKIAEKMKSDRIGSIVIFKD
                     QEPVGIVTDWDIVTDGFSRDVPPSSIIASEIMKKLYTIEGEATITEAARMLRKHNIKR
                     LGVVYKNRLVGIISSSDVIAVTPELI"
BASE COUNT          901 a          309 c          415 g          684 t
ORIGIN      
        1 tctaccaaag gaatgtgggt tactaaaatg ttattcaaac aagtagaaga aaacgattta
       61 gttagaaatg caagaaaaga acttgaaaga tcacgcataa tatccattca acaattggat
      121 tcagaaagaa tagctatttt gaaatttgaa cattatgata gtaaaattcg atatctaata
      181 tgcgaattct ttggccaagg aaatataata atttgtgacg agtccatgaa aataatttca
      241 attcttaacc cgttagaagt aagacacaga actttgcgca taggattaaa atatgcatct
      301 cctccatcat atggctcaga tatctttaaa atatccattg aagagtttaa ctcaaaattt
      361 cagaatgaaa ctaaaaatct ggaaattaca aaatggttag gacgaattct ctcattacca
      421 aaaaaatttg ttgaagaaat acttttccgc gcaaagatat atgataacag gagaaaaata
      481 aaagagatca caaacattga gcttggagtt atatttacta atacaaaaaa actggtagat
      541 gaggtaattt gtgaaaataa acattctccg gtagttattt tagatgctga caacgtacct
      601 attgacgcat ctcacgttgt attaaaaaac atggaagatt ccactatgaa accttcacct
      661 acctttatgg atgcattaga tgaggtatta tgtaatgata ttattactat tggaaaaaaa
      721 attcatactg tagaacttga taaaaaaatt tctgttcttg aacacgatct tgatgaacaa
      781 gataaggcta aactaaaagt tttatctaaa tctaattcaa taagagatct tgcttctgat
      841 ttgatgaata tatctatttc agatcctatt gcgttagagg gtttactaaa tagacattct
      901 gctaagctaa taatggaaaa gggaaaaaaa tatttagata tagtggagga acaaattcca
      961 ttagatcaga acttgagaaa agtttcttca cttctttatg caagagcgaa ggaaatggaa
     1021 agaggaagtg aaactatcga caacgctagg cttaaaatat tggatgaaat aaaagaattg
     1081 agaaaaaaaa caactattgt ggaaaacaaa attaaattaa aagaacaaaa aacaaaggaa
     1141 tggtttgaaa gatataggtg gtttgttacc tctaatggat tattagtgat tggaggtaga
     1201 gatgcctcct caaattcagc aataataaga aaacacatga ctgatcaaga tttggttttt
     1261 catgccgaaa tacatggttc tccttttttt ttaattaaga acattggtct tgatagtgta
     1321 gatgatgata ccaccactac tacaaataat caaagcatta ttgaaacagc tatagcaaca
     1381 gtaaccttta gtagaggatg gaaagaaggt ttatccagtg ctgacgcata ttgggtaaat
     1441 ccgaatcagg ttaaaaaagg ggcaccaact ggtcaatttc ttccaaaagg atcttttgta
     1501 attgaaggaa aaagaaattt tgttaaggca ttagagttaa aattagcaat aggtgtaact
     1561 aaagataatg atagttataa agtaatttgt ggacctgtcg atagcataaa atcaaaatca
     1621 ataatttatt ctgtcatagt tccaggaggt atagatccta tgaatgctac aaaaaagata
     1681 aaaacagaac taattacaaa ttcccataat atagatgatg aggatttaat tgaattttta
     1741 aaaagaatgt ccttagatga tataattagg atactacctt ctggtaaaat taaaatagtc
     1801 tcgtcagaga aaggtgcgct ttacgggaat ttgtaataat ttcttttgta gtaaactaat
     1861 taagttcgtt tatacaaatg atggatgtgg gaaccgatct aggcagcaaa catagggatg
     1921 caaatatgac aacaagggta ctagtaagag atgtcatgaa cagccccatt atttcatctt
     1981 ctcctgattc taacattaaa aaaattgcag aaaaaatgaa atcggacagg attggtagta
     2041 ttgttatatt caaggaccaa gagcctgtag gtatagtcac tgattgggat attgttacag
     2101 atggattttc acgagatgta cccccaagtt caattattgc atcagagatt atgaaaaaat
     2161 tatacacaat tgaaggagaa gctaccataa cagaagcagc tagaatgtta agaaagcata
     2221 acataaaacg tcttggggtt gtctataaaa atagattagt gggtattata tcatcttctg
     2281 atgttatagc tgttacacct gaattaata
//
LOCUS       DASVEV010000215         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1648970, whole genome shotgun sequence.
ACCESSION   DASVEV010000215 DASVEV010000000
VERSION     DASVEV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648970"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1031)
                     /locus_tag="VF222_09675"
     CDS             complement(267..1031)
                     /locus_tag="VF222_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7142733.1"
                     /translation="MNIEETANIDWNEIIKKEARGIDDADFGKVQSVESEYVSTQKGI
                     FDKEYFSFPKNLVQNYDGYKVLFKVTEEEARTSYMQKSETERLEQGGNNDYSQSNINA
                     NDKDGTETTTNIDDDDDDNDNDNDNDMSNKTRDEMIIPLMSENLEVNKTELIDDAIIT
                     KEPVRETKTIEVPVMREELILERRPVSRDPENEKEIPKPVENNTQIKIPLRREEVEVT
                     KTPYVIEELVVRKKSVTKMQTLTEEIITEKVVEPQG"
     gene            1330..2211
                     /locus_tag="VF222_09680"
     CDS             1330..2211
                     /locus_tag="VF222_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX7142734.1"
                     /translation="MESLASKTDNNNPPGKQENGNKKEDFFKEDKNPLVVSPPESETL
                     TIIIKSPALKDKESFKSRVGEAFESVRENASNIVDKVMNVEEQDINSFRTATDNKKST
                     SYSQEQEQESKEQYVIPVIGEKYALNKITLTDEINIEKRWVEQDEIKIPVRYEKVFVD
                     DKEFDAYSETHGIVSQIKDKISDLFHSSNKEDEEEKKEGSNGKSPQGIVGNSNNNDNK
                     NEGKSTTTKNYDNNQQKLIPLYAEEITISKKIVKIGEIAISKRRIVEKENVDVDMIKE
                     RINVEYGDGRKEKITPY"
BASE COUNT          928 a          355 c          360 g          864 t
ORIGIN      
        1 taaattcatt cttgaaattt caaataatga taaaacaaca aaataacaag aagaataatt
       61 gaactaaacc taggtgacat agcatgtttt gttaaacctc attcagattt gtatttctct
      121 aatttgtctt tatgaaaact atcaaaaaat ttataaccta attatatcag tataatattt
      181 ttagtgttaa tgcagattaa tagagtaaat gttaggatgg aaccacatgg ctatccttcg
      241 tttcaaaaaa atatatattt agtaaattaa ccttgtggtt ctacgacttt ttcagttata
      301 atctcttcag ttaaagtctg cattttagta actgattttt tcctgactac tagttcttct
      361 ataacataag gggttttcgt gacttcaact tcttccctcc ttaaaggtat tttgatttgt
      421 gtattatttt ctactggttt tggtatttcc ttttcatttt ctgggtctct agaaaccggt
      481 cttctttcta atattagttc ttcgcgcata acaggaactt ctattgtttt agtttctcta
      541 actggctctt ttgttatgat tgcgtcgtct attagttctg ttttattaac ttctaaattt
      601 tctgacatga gaggaattat catttcatct cttgttttgt ttgacatatc attatcatta
      661 tcattatcat tatcatcatc atcatcatcg atgtttgtag ttgtttctgt tccatcttta
      721 tcattagcat taatattact ttgactataa tcattattac caccttgctc gagacgttcg
      781 gtctcagatt tttgcatata tgaagttcta gcctcttctt cagttacttt gaataatacc
      841 ttgtaaccat cataattttg aactaaattt tttggaaacg aaaaatattc tttatcaaat
      901 ataccttttt gggtagatac atattctgat tcaacgcttt gtactttacc aaaatccgca
      961 tcatcaatcc cccttgcctc tttttttata atttcattcc aatcaatatt agcagtttct
     1021 tctatattca taactatatc tttaccataa attatataat agttggcaac aaaaacaaga
     1081 atagttttaa ataataataa tcatcaacat catatcagag tatttctcta tcagatatac
     1141 aaatgaatga atgactaaat ccaaaatagc ggattaataa acagatatgt atagcagtgt
     1201 tcaaagttca ttgatatata gatatgttgt atgtcggata tcatgaagat gagttcctat
     1261 ctaactgtca ataactttga agttgaaaca tataatctac ttaagttata tttattatag
     1321 atatatgtaa tggaatcact agcctcaaaa acagataata ataatccacc tggtaaacaa
     1381 gaaaatggaa ataaaaaaga agattttttt aaagaggata aaaatccctt agtagtttca
     1441 ccaccagaat cagaaacact aacaataata attaaatcac ctgccttaaa agataaagaa
     1501 tctttcaaat caagggtagg agaagcattt gaatcagtta gggagaatgc aagtaatatt
     1561 gttgataagg taatgaatgt agaagaacaa gatatcaatt cattcagaac tgccacggat
     1621 aataaaaaat caacttcata ctctcaagaa caagaacaag agtcaaaaga gcaatatgtc
     1681 attcccgtta taggagaaaa atatgctctt aataaaatta ccttaactga tgaaataaat
     1741 attgaaaaga ggtgggtaga acaagacgag ataaagatac cagtcagata tgaaaaagtt
     1801 tttgtagatg ataaagaatt tgatgcctac tcagaaacac atggaattgt ttctcaaatt
     1861 aaagataaga tatctgattt atttcactct tctaataagg aggatgagga agaaaaaaaa
     1921 gaaggcagta atggaaaatc tcctcaagga attgttggta atagtaataa taatgataac
     1981 aagaatgaag gaaaatcaac aacaacaaaa aattatgata acaaccaaca aaaattgatt
     2041 ccattatatg cagaagaaat taccatatca aaaaaaatag taaaaattgg tgaaattgcc
     2101 ataagcaaga gaagaatcgt agaaaaagaa aatgtagatg tagatatgat aaaggaacgt
     2161 ataaatgttg aatatggaga tggtagaaag gaaaagataa ctccttattg attattgata
     2221 aaactagaag gcataactaa tctattccta tatcaatata ttttacttca ctgaaaagta
     2281 ataattccta aaaaaataaa ataataataa ctagatataa gtacctaaaa aatagaaaag
     2341 attttggact aggttaaagt taaaacctag ttgttgctaa tatttacgtc tgaattttgt
     2401 ctattaacgg cttttatgta attggtgttt gtttttgcgc aagcctttag attatcacgt
     2461 gtttgttcta gtgctgtatt gtatactctc atatttgatg ctagagc
//
LOCUS       DASVEV010000216         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1453449, whole genome shotgun sequence.
ACCESSION   DASVEV010000216 DASVEV010000000
VERSION     DASVEV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453449"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            771..923
                     /locus_tag="VF222_09685"
     CDS             771..923
                     /locus_tag="VF222_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142735.1"
                     /translation="MKKRLLKVVVGVLNDEDFSNISLDKLKHSTKKLKVTFSMLSPIN
                     TKGKIK"
     gene            1581..>2273
                     /locus_tag="VF222_09690"
     CDS             1581..>2273
                     /locus_tag="VF222_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX7142736.1"
                     /translation="MSVSNHLLMNRTLMIYILAAIISFSALTAITMQPISLSAQIQPA
                     QQQPLAISPSCGQVITQNVVLTSNLNCAETDGLIVNAGDIVIDLNGHTISGPDIGSET
                     QASSKVGIMIPNMNNVVVQDGTIEGFQAGILMTGSQNVEVKGMVVKNNQIGLFSTGAS
                     IVNAHLSIIMNNQIGIAGHSTQQSTIEDNILFQNTLAGVTLVNSDNSVITLNSITESG
                     NGLYIDNQSNQNN"
BASE COUNT          863 a          318 c          324 g          768 t
ORIGIN      
        1 tgacaaacac aagtatagtt cagcactaat ttaatttgtt attacacaaa tatgtgatta
       61 gttgaattta gtataatcat tgcatctgga agatataaaa tcgaccattt gcgtgtacac
      121 cttgtgttat tgaattttta acgattatct tagttgtatc ttcaattgat tttatttgca
      181 ataaaaggtg aggaaatcag gaaagatggt ctagacttta acaaatgtat tccagtcact
      241 ttaaataaaa ccgagaaaaa ttcttaatgt tgatctgaaa gcaaatgtta agaattgata
      301 gagttgtata tgcttattat ccagataatt ttacctaatt gtattttact ttatacgatg
      361 gtatttttgt atctgataac gtattcataa tgggttgtta aaggtaactt taacacccat
      421 ttttcaacaa gcaaaaatga atagaaccta aattcttctt gttgcagatt ctgatatttt
      481 actatccatt ttttataata aatagttaac taatagtacc tagtccaaaa aataataaat
      541 agtatattaa aaatatttca tttgtattaa aaattcctgg taatcttctt tcattatgta
      601 aagtatcttt ggaaaaatta gtaatagtgg cattgtttat tgttggctgg agtattacat
      661 ttttaactat agataaagtt atggcacaag agtcagaaat tatttaaaca tatggaaggt
      721 aaaaataact aactaattaa aagttatatc aaatattaat ttacaatgta ttgaaaaaaa
      781 ggttacttaa agtagttgtt ggtgtcctta atgatgaaga tttctcaaat atttctttag
      841 ataaattaaa gcattctacc aaaaaattaa aagttacatt ctctatgcta agtcctatta
      901 atacaaaagg taaaattaaa tgaccaatag cagtaccctg ttggcaagca aactacggtt
      961 tagaagataa tatccaatac tacctccaac tgcggttata attataaata tgacaactaa
     1021 aatgactatt tgacccattg tattgttgct agcaaatgta ttgttactag caaataattt
     1081 aatataaatt cctatattgt caccaccatt tgaaaaagtt acagcagcaa cactttaaaa
     1141 gataatgctg agttagtttt tgttaattta tattttgttg aatttgtaat tggttctttt
     1201 tttcttttta taattttcta ttaatttttt tataccaatt ataataagtg tatactcata
     1261 aaacctataa taaataatgg aattcctaag gagataaaag aagcaattta tactgattgc
     1321 tactaataat ccaattccaa tatatagtcc aattacaact tggtaaggtg gaaattttag
     1381 agttagaaaa gaacacaatt aacaaaaaaa atatcatcaa tgttagtaga gacaaataca
     1441 gatatgcata aaactatcaa atatccaaag cctaccaatc catttttcta ttgaggtagt
     1501 aatatagatt gaatttcaaa taaaaggaat aataaaataa tttaaactaa aataacggtt
     1561 ctttacggat taacataaat atgtcagtat caaaccatct actaatgaat agaacactta
     1621 tgatctatat attggcagcg ataatctcat tctctgccct tacagcaatt acaatgcaac
     1681 ccatatcatt aagcgctcaa atccaaccag cacaacaaca accattagcc ataagtccat
     1741 catgcggtca agttataaca caaaatgttg tattaacttc taacttgaat tgtgcagaaa
     1801 ctgatggtct tatagtaaat gcaggagata ttgttataga ccttaacgga catacaatat
     1861 ctggtccaga tataggtagt gagacacagg cctccagtaa agtaggaatt atgataccaa
     1921 atatgaacaa tgttgtagtt caggatggta ctattgaagg atttcaagct ggtatactaa
     1981 tgacaggttc tcaaaatgtt gaagtaaaag gaatggttgt taaaaataat caaataggct
     2041 tattcagtac tggtgccagt atagttaatg cacatctgtc cataattatg aataaccaaa
     2101 ttgggattgc aggacactct acacagcaaa gtactattga agataacata cttttccaaa
     2161 ataccttggc aggagtaaca ttagttaact cagataactc tgttatcact ttgaattcta
     2221 ttactgaatc tggaaatggt ctttatatag acaatcaaag taaccaaaac aat
//
LOCUS       DASVEV010000217         5976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1065578, whole genome shotgun sequence.
ACCESSION   DASVEV010000217 DASVEV010000000
VERSION     DASVEV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065578"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..454)
                     /locus_tag="VF222_09695"
     CDS             complement(290..454)
                     /locus_tag="VF222_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142737.1"
                     /translation="MHSRRNVVITCYVYFMTYIAGKESTLFLVDVENELSLGCKIKKH
                     SEEKKELISK"
     gene            complement(957..1781)
                     /gene="pheA"
                     /locus_tag="VF222_09700"
     CDS             complement(957..1781)
                     /gene="pheA"
                     /locus_tag="VF222_09700"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7142738.1"
                     /translation="MISKGIRIAFQGERGAYSEMAIMRNFPNSIAIPLKTFHDVFETV
                     KNDLSQIAVVPIENSIEGSVNETYDLLLQDKILVSGEIFQRINHCLIVNKDYKKIIDV
                     YSHPQALGQCRKYIQSKKLEPIPAFDTAGAVKFIKDNKMRQSAAIASRRAAEIYEMKI
                     LEEGIEDKKNNFTRFFVISKHSLDKPSGKDRTSIIFALEHQPGSLYNVIKEFSQERIN
                     LTRIESRPTKETPWEYYFYVDFDGHHKDEKIMRTLNRIKTRTKLFKLLGSYHKGIF"
     gene            complement(1895..3727)
                     /locus_tag="VF222_09705"
     CDS             complement(1895..3727)
                     /locus_tag="VF222_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HEX7142739.1"
                     /translation="MNTKKYVLDTSIIIDKEISKMLQSGKIVENSEIIIPRAVLDELQ
                     AQASTSRDQGFVGLQEIHEIRDVCNKKGIKLKFSGKRPDYDDVLLAKHGRIDSIINEL
                     AFEEDAILITGDYVQHLAALAQGIDSIFIKYNNKITHFKFEKFFSEKTMSIHLKEGVC
                     PYAKIGSPGKFELRRLDDKIITNEYLKEIIKEIYEYNPLNTNKLYEINREGAAVIQYE
                     KYRITITRSPFSDSTEITIVRPIVQLSLDDYKLSEKLVRRLEEKAEGIVIAGPPGSGK
                     STIASSLAEYYTKKAKIVKTFESPRDLQVPKEVTQYSPLEGSFENAVDLLLLVRPDYT
                     IFDEIRRIKDFNVYADLRLSGVGMIGVIHANSPIDAVQRFIGKIELGMIPHIIDTIIF
                     LKDGNIKKVYELKLTVKVPSGMVEEDLARPLVEIFDFENGELEYEIYTYGEENIVVPV
                     KELVEKKRTNTGSNITRLAESKINDVVKRFDPNADIEIISDNKVRIKVGKEIIPKIIG
                     KSGSTVSELEKLLGLRIDIEPKIPVLGKGVDFQINESGSSILIITDNKEVGSNVGIYI
                     NNEFLLSSQIGRKSRIKVDKRSEYGKKLLNAILTSSDIKLFKTG"
     gene            3890..4414
                     /locus_tag="VF222_09710"
     CDS             3890..4414
                     /locus_tag="VF222_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142740.1"
                     /translation="MTILSKENAKARLEEFRILLRYMPFLQKNGFSDCGRGQIMGQMS
                     LIQEFLSETQTSDEILNLQYKDPVISNASNINNNTSHSISDIPQYYTSTTNETDKQFT
                     DGSKDNTFDLDSIILENDTSSEEDSKLQSSRWLRPFKNEDPIKKKLFENFEKNEITQS
                     WKDKIKKMTQSDII"
     gene            complement(4451..5608)
                     /locus_tag="VF222_09715"
     CDS             complement(4451..5608)
                     /locus_tag="VF222_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7142741.1"
                     /translation="MASEDIYIKGFATSEGTKKFRDNAVKKGKSYLHFKEFDDLILSS
                     IGIGTYLGDLSKKDDKDIEIALYESVKSGAINVIDTAINYRAMKSEKSIGRSITKLVN
                     DGIISRDAIFVSTKNGYITNDGDYPMLDVLGYIQRMYIDTGIIKAEDISSGYNVLNPA
                     YIEKCIERSRFNLKLDTLDLVYIHNAFESWNQDVSKNKFFDMLSKVFEIYEQFRSKNK
                     IRYYGMATWTCFRVGEKNKEYLSLDEVYNIAKNIGGADHGFRFIQLPYNLAYSEALFL
                     KNQNVGIEKNLTILEAAKRLKIGVFSSVPLLQGKLIQSKIPDYSEGLTDPVMKLLQII
                     RSSPSIIAPLIGQKKMDHVNKNNKISEIPPLNEEEFKLAIEVLTQGNEQLL"
     gene            5725..>5976
                     /locus_tag="VF222_09720"
     CDS             5725..>5976
                     /locus_tag="VF222_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142742.1"
                     /translation="MPSNSSGSNNNSNNNNNSSSGNSGGGGSAGAGGGGGAAASAGAG
                     VAGGNNTSLKVLEAYTRDVGRGIARIDYDTMDSLAVSTGD"
BASE COUNT         1905 a          972 c          768 g         2331 t
ORIGIN      
        1 aaaataatga ctctttatct caacaaaatc ctaatccgga aattcctact attcaagaaa
       61 aaatgaatag tagtgtaaat acaacaaatg ataagaaaag tccagatgaa ggattatttt
      121 ttgggttatt taaataggta aatggtaaaa cagtataata tcgaacagaa taactacgta
      181 ttgatttttc tacagatatc tacaatattg taattgtaat atttggattt tatttaataa
      241 tattttcaac tggaaattta tgccctaatg gaatcaacaa aaagatttat cactttgata
      301 ttaattcttt tttctcctca ctatgttttt ttatcttaca acctaacgat agctcattct
      361 ccacatctac tagaaatagt gttgattcct tacccgctat atatgtcata aaatagacat
      421 aacatgttat tactacattt ctacgagaat gcatatagtt actccaaaat attctgccaa
      481 aactctacca aaccaacaaa taaagcagat ccatattgaa ataatctgtt tttggtttcg
      541 gaactataaa atgccaaacc atatttttag tataatcatt atttctctga tcaaaaaact
      601 gaaagtctga ttatcatatt gtggtgaatc tgataatata tattatgaaa aatcatccat
      661 tccatttgaa tcgaaatatt tctaataatc ttatacttcc ttgtgaatta atgaattcca
      721 aatatttgtt tcccttaccg ataaagacat taactctaca attgatttta tgatatctac
      781 atttatcata ttgtagatat atttggacac aagacagaat aaacttttct gaactcaata
      841 ataaaaatga tataacaaat gataaattaa aaaaggagaa atttctctac atttcataag
      901 aactaataat ttattattat ttattttgtt atagtagatt atataaatat aataatttaa
      961 aaaattcctt tatgatagga tcctagtagt ttaaaaagct ttgttcgagt tttaattcta
     1021 tttaatgtcc gcattatttt ttcatcttta tgatgcccat caaaatctac ataaaaatag
     1081 tattcccaag gagtctcttt tgtaggtcga gattcaattc tagtaagatt tatcctctct
     1141 tgagaaaatt cttttattac gttgtacaaa gaacctggtt gatgttctaa tgcaaaaata
     1201 attgaagttc gatcttttcc tgatggttta tctagtgagt gtttcgatat aacaaagaac
     1261 cttgtaaaat tatttttttt atcttctatt ccttcttcta aaatcttcat ttcatatatt
     1321 tctgctgctc tcctactcgc aatagctgca gattgcctca ttttgttatc cttgatgaat
     1381 tttactgctc ccgcagtgtc aaacgctgga attggttcta attttttact ttgaatatat
     1441 tttctacatt gtcctaatgc ttgaggatgt gaatatacat caattatttt tttataatcc
     1501 ttatttacaa ttaaacaatg atttattctt tgaaagattt ctcctgaaac gagtattttg
     1561 tcctgtaata gtaaatcata ggtttcattt acgcttccct cgattgaatt ttcaataggg
     1621 accactgcta tttgacttag atcattttta acagtttcaa aaacatcatg aaaagttttt
     1681 aaaggaatag caatagagtt agggaagttc cgcattattg ccatttcact atatgctcct
     1741 ctttctcctt gaaaggcaat ccttattcct ttactaatca aataattaat tgataatagt
     1801 aaaacaatct tttatgtgta ttctaaacta aatcttgcaa atatgagtat acatggttat
     1861 cgaaattttt tagttttaat ttctttgtta tttatcaacc tgttttgaaa agtttaatat
     1921 ccgatgatgt caatattgca ttcagcagtt tttttccata ttctgatctt ttgtcaactt
     1981 tgattcttga ttttcttcca atttgtgaac ttaacaggaa ttcgttatta atataaatcc
     2041 caacattgga tccaacttct ttattatctg taataattaa gattgatgaa cccgattcat
     2101 ttatttggaa atcaactcct tttccaagaa cgggtatttt aggttctatg tcaattctta
     2161 aacctaatag cttttctagt tcagagacag tcgaaccgct ttttcctatg atttttggaa
     2221 ttatttcttt tcctactttt attcttactt tgttatcaga tataatttct atatctgcat
     2281 taggatcaaa cctctttact acatcattta tcttagattc tgcaagtctt gtgatattac
     2341 tcccagtatt tgtccttttc ttttctacta attcctttac aggtactact atattttctt
     2401 caccataagt atatatttca tattctaatt ctccattttc aaaatcaaaa atttcaacta
     2461 atggtctagc tagatcttct tcaaccattc cagacgggac tttaactgtc aattttaatt
     2521 cataaacctt ttttatatta ccatctttaa gaaaaataat tgtatctata atatgaggaa
     2581 tcatgccaag ttctatcttt ccaataaacc tttgaacagc gtctattggg ctattcgcat
     2641 gaattactcc aatcattcca actcctgata atctcaaatc tgcatataca ttgaagtctt
     2701 ttatccttct tatttcatca aaaatagtat aatctggtct aaccagtagc agtaaatcta
     2761 cagcattttc aaaacttcct tccaacggac tatattgtgt aacttctttg ggaacttgta
     2821 aatctcttgg tgattcaaat gtttttacaa tttttgcctt ttttgtataa tattctgcta
     2881 aactgcttgc tattgtactt ttacccgatc caggagggcc agctataaca attccttcag
     2941 ctttctcttc taatcttcta acaagtttct cacttaattt gtaatcgtct aaagataact
     3001 gtactatcgg tcttacaata gtaatctctg tagaatccga aaatggagat ctagtaattg
     3061 ttatcctgta tttttcatat tgaataacgg cagctccttc tcgatttatt tcatataatt
     3121 tattagtatt cagtggatta tattcgtaaa tttcttttat tatttcctta agatattcat
     3181 ttgttataat cttgtcatct aatcttctca attcaaattt tcctgggctt ccaatttttg
     3241 catatggaca aactccttct tttaggtgga tactcattgt tttctctgaa aaaaattttt
     3301 cgaatttgaa atgagttatt ttattattgt attttataaa aattgaatct attccttgtg
     3361 ctagtgctgc taaatgttgt acataatcac cagtaattag aattgcatct tcttcaaatg
     3421 ctagttcatt aattattgaa tcaattcttc catgctttgc taataataca tcatcataat
     3481 ccggtctttt gccagaaaat ttcagtttaa tacctttttt attacataca tctcttattt
     3541 catgaatttc ttgcagacct acaaaacctt gatctctgct agtggatgcc tgagcttgta
     3601 attcatctaa aactgcccta gggataatta tttcactatt ttcaactatt tttccactct
     3661 gtaacatttt agaaatttct ttatcaatta ttatactagt gtctaaaaca tattttttgg
     3721 tattcaatta ttttagtaat tgtaaatctt tatccgtact taaatatatt ttaaatcttt
     3781 taccttcata cgccattatg actacatgtg ctagctagct tttagaatgt gaattagcaa
     3841 aataattgaa ctagccttaa tttaaaagaa tcaaaccatt tagaatgaaa tgacgatatt
     3901 atctaaagaa aacgccaaag ctcgattgga agaatttaga atattgctca ggtacatgcc
     3961 ttttctacaa aagaacggct tttctgattg tggtagaggt caaattatgg gtcaaatgtc
     4021 gttaatacaa gaatttcttt ctgagacgca aaccagtgat gaaattttaa atttgcagta
     4081 taaggatcct gttatatcca atgcatcaaa tattaataat aatacttctc attcaatttc
     4141 tgatattcca caatattata catctaccac taatgaaaca gataaacaat ttactgatgg
     4201 ctcaaaagat aatacttttg acctagatag tataatatta gagaatgata cctcttctga
     4261 agaggatagt aaattacaaa gttctcgctg gttacgacca tttaaaaatg aggatcctat
     4321 aaaaaagaaa ttgtttgaga atttcgaaaa gaatgaaatt acacaatcat ggaaagataa
     4381 aataaaaaaa atgacacaat cagatattat ctaattctaa aatcttcttt tttttatact
     4441 atctcaatca ttataataat tgttcatttc cttgagtaag tacttcaata gctaatttga
     4501 attcttcttc attcaaaggt ggtatttctg aaattttatt atttttattt acatgatcca
     4561 tttttttctg acctatcaac ggagctatta tagaaggact tgatcttatt atttggagga
     4621 gtttcataac cggatctgtt aatccttccg aatagtctgg aatcttactt tggattaatt
     4681 ttccttgaag taatggaaca ctagaaaata ctcctatttt caatcttttt gcagcttcta
     4741 aaattgtaag gtttttttca attcctacat tttgattttt caaaaataac gcttcactat
     4801 atgctaaatt gtatggaagc tggataaaac gaaatccatg atctgcacct cctatatttt
     4861 tagcgatatt gtacacttca tccagtgata aatattcttt atttttttca cctactctaa
     4921 aacatgtcca agtagccatt ccataatatc taattttatt ttttgagcgg aattgttcat
     4981 aaatttcaaa aactttggaa agcatatcaa aaaatttatt cttactaaca tcctgattcc
     5041 aactttcaaa agcattatgg atatatacta gatctagtgt atcaagtttt aagttaaatc
     5101 gtgatctctc tatacatttc tctatgtagg cagggttaag aacgttatat ccagaactta
     5161 tatcttctgc ttttatgatc ccagtatcta tgtacattct ttggatatat cccaaaacat
     5221 caagcattgg gtagtctccg tcatttgtta tatatccatt ttttgtactt acgaaaattg
     5281 catctcttga aattattcca tcgtttacaa gtttagttat tgatctgcca atgctttttt
     5341 ctgatttcat tgctctataa tttattgcag tatcaataac attaattgca cctgatttta
     5401 ctgattcata tagtgcaatt tcgatatctt tatcatcttt tttcgagaga tcacctaaat
     5461 atgtacctat tccaatactt gataatatta gatcatcaaa ttctttaaaa tgtaaatatg
     5521 atttaccttt tttaactgca ttgtctctaa attttttcgt tccttcgctg gtagcaaatc
     5581 cttttatata gatatcttca ctcgccaata agttataata ttgttgacta ttattatttt
     5641 tttattcttt tttttatcat aatttttatc agacaaagat gtatatgtag tcaattcacc
     5701 taaggtatac aagtgaacag tagtttgcct agtaatagta gtggcagtaa taataatagt
     5761 aataataata ataatagtag tagtggcaac agtggtggtg gtggtagtgc gggtgctggt
     5821 ggtggtggtg gtgctgctgc tagtgctggc gcaggagtag cgggaggaaa caatacatca
     5881 ctaaaagtgc tggaagctta tactcgtgat gttggtagag gcatagcacg aattgattac
     5941 gataccatgg attctttagc agtttctact ggtgat
//
LOCUS       DASVEV010000218         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1260357, whole genome shotgun sequence.
ACCESSION   DASVEV010000218 DASVEV010000000
VERSION     DASVEV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260357"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..709)
                     /locus_tag="VF222_09725"
     CDS             complement(104..709)
                     /locus_tag="VF222_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142743.1"
                     /translation="MALSLYKLAFPAILALLAVGTAGFLLAFSYYPEKHVNVDIDGKC
                     YELLGISNEKHKILRAENEINVKQMQLNMIEHGDTSLPIVFSGFKGDVDDFINQYDIR
                     NILSVQKVSDNPTFDKFIIKATLTKNELQQIVNDLAISDFYPTLSVKGSVGLGPNKYI
                     SSEEGKIISTESKKFMQKGIKNIVESNIEVEGIKLAECRNI"
     gene            complement(732..1058)
                     /locus_tag="VF222_09730"
     CDS             complement(732..1058)
                     /locus_tag="VF222_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142744.1"
                     /translation="MNQLKNIDFFELDRVLEDVAKTYAAPSATIWFKVNNNSKPTQEE
                     YHEKVVEFIKKFEDLIKSNFPSENEHSDYLKEYISKNIRYGISSINAGNNKEVERRYR
                     YYVDYN"
     gene            1196..2230
                     /locus_tag="VF222_09735"
     CDS             1196..2230
                     /locus_tag="VF222_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7142745.1"
                     /translation="MEINKNEFNVSNLLLDEIDKAILVHLGKNARISSYDIAKHLNDL
                     GYDITDRTIRNRLKRLETSNVILGYSSILNPDIIQDKINRTVLLKFKYSLNTSSLIKR
                     LEQYCEESQFCIYAAKTTGDFDWVCHFVFDSVDQFELESNNFVNRFIELIQDYRSYES
                     KTIKSMSYTIFDDYESQEKKFQVYKILNSLKKYNNLNDKLQFIVESIVKYFDAKFARL
                     WILDKEQRNLVLKFSAGKYKNINGEFSIIPIDENKIGPIVKTKKPTITNDIVNDKRIK
                     YPNWAKQEKLQSFAGFPLTHKDRGIGVIAMFSTKKLQASDFEILDIFSNHVSKELSEL
                     FDTIEFFNLK"
     gene            complement(2710..>2922)
                     /locus_tag="VF222_09740"
     CDS             complement(2710..>2922)
                     /locus_tag="VF222_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7142746.1"
                     /translation="GTLKSAVELKKLYESLNVTPDKEVITYCGIGERAAYTWFVLKYL
                     LGYPNVKSYDGSWLEWGNTIGNTIEK"
BASE COUNT         1030 a          420 c          376 g         1096 t
ORIGIN      
        1 aacaaaaacc ttaacagata tatttatgtg aaaaatccta tatcgatatt ttaagtacgg
       61 tataagtatg acagcaaaaa gaatagatga ttactaactt caactaaata tttcgacatt
      121 cagctaattt tattccttca acttctatat ttgattcaac aatattttta ataccttttt
      181 gcatgaattt tttagattct gttgaaatta tttttccttc ctcgctacta atgtatttat
      241 ttggtcccaa cccaacactt ccttttactg ataacgttgg ataaaagtca cttattgcca
      301 gatcatttac tatttgctga agctcattct ttgttaatgt tgcttttatt ataaatttat
      361 caaatgttgg attgtcagaa accttttgta ccgataatat gtttctaata tcatattgat
      421 ttatgaaatc atctacatct cctttaaatc cactaaaaac tattggcaaa ctggtatcac
      481 catgttcaat catatttaat tgcatttgtt tgacattaat ttcattttca gctctgagaa
      541 ttttatgctt ttcatttgat atacccaata attcataaca ttttccatca atatctacat
      601 ttacatgttt ttcaggataa taactgaatg caagtagaaa acctgctgtt ccaactgcta
      661 ataatgctaa tatagctggg aatgcgagtt tgtatagact taaagccatt tcttttattc
      721 atccttacct gttaattgta atctacataa tatcgatacc ttctttctac ttctttgtta
      781 tttccagcgt taattgaaga aattccatat cttatatttt tggaaatata ctctttcaag
      841 taatctgaat gttcattttc tgatggaaaa ttagatttta tcaaatcttc aaatttcttt
      901 ataaattcaa caaccttctc atgatattct tcttgtgtgg gtttgctatt attatttact
      961 ttaaaccata tagtagctga tggtgcagca tatgtttttg caacatcttc taatactcta
     1021 tctaattcaa agaaatctat attttttaat tgattcataa ctatattacg tcatttgtaa
     1081 taatattccc tgcggaagaa tttattgttt ttattttcca ctttggaaaa taggtattat
     1141 ttttatctaa ggctttaatt attatttgta agaattataa tgattatttt cctaaatgga
     1201 aataaataaa aatgaattta acgtatctaa cttattgctt gatgaaatcg ataaagctat
     1261 tctagtacat ttaggaaaaa acgcacgtat ttcatcatat gatattgcta aacaccttaa
     1321 tgatttaggt tatgatataa ccgatagaac tataagaaat agattaaaaa gattagaaac
     1381 atcaaatgta attttaggct attcatcaat tttaaatcct gatattatac aagataaaat
     1441 aaatcgaact gtgttactta aattcaaata ttcactgaat acttcctcct taattaaaag
     1501 acttgaacaa tattgtgaag aatcgcaatt ctgtatatat gcagccaaaa ctactggtga
     1561 ttttgattgg gtatgtcatt ttgtatttga ttcagttgac caatttgaac ttgaaagtaa
     1621 taattttgtg aataggttta ttgaacttat acaagattat cgctcttatg aatcaaaaac
     1681 catcaaatct atgtcatata cgatatttga tgattatgaa agccaagaaa aaaaatttca
     1741 agtttataaa atcttaaatt cacttaaaaa atataataat ctaaatgaca aacttcaatt
     1801 tattgtagaa agtatagtaa aatattttga cgcaaaattt gcaaggctat ggattttaga
     1861 taaagaacaa agaaatcttg tattaaagtt tagtgctgga aaatacaaga atatcaatgg
     1921 agaattttct attattccta ttgatgagaa taagattgga ccgattgtaa aaacaaaaaa
     1981 accgactatt actaatgata ttgtaaatga taagcgaata aaatatccta attgggcaaa
     2041 gcaagaaaaa ttgcaatctt ttgcaggatt tccacttaca cataaggata gaggtattgg
     2101 tgtaatagct atgtttagta ctaaaaaact tcaagcttca gattttgaaa ttcttgatat
     2161 attttcaaat catgtttcaa aagagctttc agaattattt gatacaatag aattctttaa
     2221 cttgaaataa tgagtcttat actttaagta gaaatttgat tgaagaaaaa aataaaatct
     2281 tgtcatgcat gtattgataa ttttcatata tatgttccta ctaatagtaa ttccgatggc
     2341 ttatcggtta caacaatttt tctaagggaa ttgtatttct aataatagta tttcctttaa
     2401 tagtgactac tggagtcaag cgccggagtg ggactcgaac cctgcaacta cattcgcggg
     2461 tttatccaat tttgcaaata ttaaagaaaa ttgacttttc taaatgtacc acaattagaa
     2521 agacggaatg aaaaccatga caaaagtaaa attcaaatac atttgtttat tttattttta
     2581 aaattacaaa atatcttttt tgattcaata tttgaacgga atattttttg taattattat
     2641 ttggtagtgg gaaaaccttt aatttttaaa atatatcccc tatataagag aaacatcaaa
     2701 gaaatttttt tatttttcaa tagtattgcc tatagtatta ccccattcta gccatgagcc
     2761 atcgtaactt tttacattag gataaccaag aagatatttc aaaacaaacc aagtataagc
     2821 tgctctctca ccaatcccgc aatatgttat tacttcttta tcgggtgtaa catttagtga
     2881 ttcataaagt ttcttcaact ctactgccga cttgagggtg cc
//
LOCUS       DASVEV010000219        14453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1652541, whole genome shotgun sequence.
ACCESSION   DASVEV010000219 DASVEV010000000
VERSION     DASVEV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652541"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VF222_09745"
     CDS             <1..950
                     /locus_tag="VF222_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142747.1"
                     /translation="LVGTQQRGLALGLNEFAGYVSVALIGFLTGYIAAVYGLKPYPFY
                     LGIIFPIVGFLISFLLVKDTKEFTQLEIKNYQKDEKRDEENEKMIESIDDEDDDEEKT
                     HTIASNSYLPNKKSKNLNFKEVFIVTSWKNRSLLAVSQAGLVNNLVFGVSWGLFTLYF
                     LSLEVSVNDTAFLKALHPGVWGFLQLATGPFSDLVGRKKMIYPGMIMQAIGIWLILLS
                     QDTYFGILVGMSLFGIGTAFVYPALLAAISDIAHPQWRATSLGVYRFWRDLGFVIGAL
                     GIGFIADFFSISMAIQIAGWIALGSGIFVFIVMHETKRN"
     gene            complement(1199..1438)
                     /locus_tag="VF222_09750"
     CDS             complement(1199..1438)
                     /locus_tag="VF222_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142748.1"
                     /translation="MHLLSLFIEEDVKYGCYAANLTPILFFNTILILVLVLVIVFSVA
                     SLVFEIVIVVIKILIVKINMFSDDGVYRDGRECTN"
     gene            1564..1767
                     /locus_tag="VF222_09755"
     CDS             1564..1767
                     /locus_tag="VF222_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142749.1"
                     /translation="MQEKVACPICKLLGFKSLVYEIDWKSHFQKFGGYYDENGNYIES
                     DIVPSPLVSKTFWCTYGHSWIQT"
     gene            complement(1970..2110)
                     /locus_tag="VF222_09760"
     CDS             complement(1970..2110)
                     /locus_tag="VF222_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142750.1"
                     /translation="MTIPIVLLELTQYYDRYRNLWEDPEYQKKYSLSTKNNNDENEDI
                     TF"
     gene            2461..2784
                     /locus_tag="VF222_09765"
     CDS             2461..2784
                     /locus_tag="VF222_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142751.1"
                     /translation="MRIQPIKVANFALEGQGWKPMLESNYSSSTSSLDVVEVILKAVK
                     SNNPEIRYLVGNDALFLIEKRQNITDKEFENWMKESLFEQKGFVVEWKARVQAFLSSI
                     NWYNI"
     gene            2947..4023
                     /locus_tag="VF222_09770"
     CDS             2947..4023
                     /locus_tag="VF222_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142752.1"
                     /translation="MKTRLDLYFSAIMITRTTFGISFSFFLNNTNPLVYGMETYNYST
                     VEINTKLFLEKLQQSGGPPIYTLTPSEARNVLSGLQAGPIEELPAEIENKTIPGGPNG
                     EISIQILKPPGTGNETLPVVMYFHGGGWVLGGFDTHERLLRELANGAHAAIVFVNYTR
                     SPEAKYPIPIEEAYAATKWISENGQTLNLDSSRLVVAGDSVGGNMATVVALLAKERGG
                     PPIIFQLLFYPVTDANFDTPSYLKYQEGYWLTRDAMKWFWDNYLSNDTNRKEPTVSPL
                     QASIEQLSGLPPALIIVDENDVLRDEGEAYAHKLMQAGVPTTATRYLGTIHDFVMLNV
                     LKDTPASRGATDQASQTLKEIYSK"
     gene            complement(4233..4964)
                     /locus_tag="VF222_09775"
     CDS             complement(4233..4964)
                     /locus_tag="VF222_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7142753.1"
                     /translation="MANHNNSNRYDFSNKIVLVTGGTGAMGRSITKAFLGSNATVISS
                     YHSDKETSQIQTENKPTVQLFKANVTNEEEVEKLVSNILDKYGRIDILVNVVGAYLGG
                     KAIVDLEEKEWDMMMTVNLKSAFLISKHTAKQMISSKYGKIIHISSKIGLHSEGYDSA
                     YAASKSGLIRLVESLSNETKESNINVNCIMPTIIDTEANRKAMPNADFNKWVKPEDLA
                     NVILFLSSEEAKTITGAAIPTFGIS"
     gene            complement(5168..6568)
                     /locus_tag="VF222_09780"
     CDS             complement(5168..6568)
                     /locus_tag="VF222_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7142754.1"
                     /translation="MTNSELTTINPSTEEILNRYTIMTKEEVNTIVKKAQDAYKEWDK
                     DLDKRIDSLYDVSKELRKSKENLSRTATNEMGKAIKEARAEVDKCVWAIEYFADNGKN
                     FIHDESFNTDARKSTIKFEALGVIGSIMPWNFPYWQALRFAAPSLVVGNTIVLKPASA
                     TMQCGIEIENIFKKAGLFDGIFNTLIGDSTVAEILVDSPDVNAVTFTGSVPVGAKVAQ
                     RATSQLKKTVLELGGSDPFIVCADADLEKASTGAVKGRFINCGQSCIASKRFIVVKNI
                     ANVFIEKFIQKTEKLKVGDPSSEDTDIGPLVNAKALETIDSQVNDSINEGAEVLTGGE
                     KIGSKGYFYKPTILGNITPNMRIVNEEVFGPAAPILIADNEKEAIKLANNSQFGLGAS
                     IWTQDLENAEVLSSIIKSGIVSVNNVVVSDPRVPFGGVKKSGFGRELSRYGMLEFVNI
                     KSIRLYDQLIHEHYVE"
     gene            complement(6565..6714)
                     /locus_tag="VF222_09785"
     CDS             complement(6565..6714)
                     /locus_tag="VF222_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142755.1"
                     /translation="MSDLIELVSLVKRNIIKSVISNRFRLEEATKALRMLKDGKILGR
                     SVIYP"
     gene            7297..8352
                     /locus_tag="VF222_09790"
     CDS             7297..8352
                     /locus_tag="VF222_09790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7142756.1"
                     /translation="MNNNDFSNYLMDIINKGSLSIMLSIGHRTKLFDILSILPPSTVE
                     EIASKSKLNERYIREWLGAMVTGKIIEYDSINNKFWLSKEKAQYLTRENNIYNFAASM
                     QWIPILSQVEDEIVECFVKGGGVPYSSYSRFHEVMAEESYQTVVIGLINNILPLVPNL
                     IADLRNGIRVLDIGCGKGKAVNLMGKHFPKSVFYGYDLSNEAIDDATIEAKDMNNSNV
                     FFKIQDILNLTFNDKFDLITAFDAIHDQPKPDLVLKNIYNSLSAKGGVFLMQDILAST
                     PLKDNISHPLGTFLYTISCLHCMSVSLSQNGAGLGAMWGKEKAVFMLKEAGFANVEVK
                     TLPHDFQNYYYICQRNK"
     gene            8577..8963
                     /locus_tag="VF222_09795"
     CDS             8577..8963
                     /locus_tag="VF222_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7142757.1"
                     /translation="MINKMDSSSSKIQKKNLDSSPDDTRKFEKGKVEIVNFGNMTIGR
                     AIFEPGWSWSKCIKPIVNTNSCQAPHTQYVISGRLKVVMDDGTEEEFGPGDAAIIPPG
                     HDAWVVGNESVVAIDFTGLKDYAKKE"
     gene            10616..11011
                     /locus_tag="VF222_09800"
     CDS             10616..11011
                     /locus_tag="VF222_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142758.1"
                     /translation="MSILSKTLTLRIPSNIIYRALKDTRLEKFFPEFFIGIERKVIID
                     KINKEISFNTITQGRQLEIIETFKFKISSNNETVVKYITETKSIEGNNIVLESIVQTH
                     VANILYSLLMLETGYINGVLEKKKTADSI"
     gene            11409..11816
                     /locus_tag="VF222_09805"
     CDS             11409..11816
                     /locus_tag="VF222_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX7142759.1"
                     /translation="MAINKETKSLGMPWEKEYGYSQAVKVRDTIYLSGQVSHDDEGNI
                     VGLKDMETQMRQAYANIKKVLAQYGATMDNIVDEVLFVTDMKSAFAAAVKCRREIFSS
                     TPIVASTIVQINGLAFPEFMIEIKCVAKLETSL"
     gene            12404..12934
                     /locus_tag="VF222_09810"
     CDS             12404..12934
                     /locus_tag="VF222_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142760.1"
                     /translation="MINNLLTILVVAASLSVLTLNSISIIGVTESYSQTAETQAKSQS
                     SNTIHITKDASNSYVLSSGSSQIGTFDTTYTIAGSVTSLKESNDLVTSTIIKDFDKSP
                     VIGYVIAHTSKSNSKLPTLANPFVDKTTINEKIKSEIQSSIGSSQKLKTTQAEIKCNF
                     GMEIKDWKCSTHGLLG"
     gene            13131..13727
                     /locus_tag="VF222_09815"
     CDS             13131..13727
                     /locus_tag="VF222_09815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7142761.1"
                     /translation="MLKIKPITLEGHQTILRPPSLKDVKGLSLAAQDGEIWNTRFAYF
                     PHPNEMSKYLKELLNGSSPGKFLPFITVHKLSNTIVGTTRYLNIDCENHRLEIGHTWI
                     AKSWRRTSINTEAKYLMLEYAFEKLGCIAVEIRTDVLNIVSRKAIQRLGAKQDGILRY
                     HKIMHDGRIRDTVCYSIIEPEWKSIKKKLIEKMHQYNE"
     gene            complement(13914..>14453)
                     /locus_tag="VF222_09820"
     CDS             complement(13914..>14453)
                     /locus_tag="VF222_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142762.1"
                     /translation="NINRTKLNTDNTNTTNFNERTNRDYNNTLNQQQDAINKTLDNTL
                     QNVKRTTDDAAREIPRYTQRVAEYQEQTIQTIKDIASDFIETEKQVIGSFQSQVNRNS
                     GVWDLYNSQRIAENHAVMVNHFTSYLLDNSNLINNALASNMRVYNTALEQTRDNLKAC
                     AKTNANFIQSVNRQNSRKY"
BASE COUNT         5047 a         2075 c         2221 g         5110 t
ORIGIN      
        1 atttagttgg aacacaacaa cgaggattgg cattaggact taatgaattt gctggatatg
       61 tatcagtcgc attaataggt tttttaactg gttatattgc cgcagtttac ggattaaaac
      121 catatccatt ttaccttgga atcatatttc cgatagttgg atttttaatt tctttcttat
      181 tagttaaaga cacaaaagaa tttactcaat tagaaataaa aaattatcaa aaagatgaga
      241 aaagagatga ggaaaatgaa aagatgatag aatcaataga tgatgaggat gatgatgaag
      301 aaaaaacaca cacaatagct agcaattcct atttaccaaa caaaaaaagt aaaaatctta
      361 actttaagga agtattcatt gtaacatcgt ggaagaatcg ttctctttta gcagtgagtc
      421 aagcaggact tgttaataat ttagtatttg gtgtatcctg gggtttgttt acactctatt
      481 ttctatcttt ggaagtaagt gtaaatgata ctgcattctt aaaagcactg catcctggag
      541 tttgggggtt tttgcaactt gctacaggtc catttagtga tttagtagga cgaaaaaaaa
      601 tgatttatcc aggaatgatt atgcaagcaa taggaatttg gctaatactg ttatctcaag
      661 atacctattt tggtatactc gttggaatgt ctctctttgg tataggaact gcatttgtgt
      721 atcctgcttt actcgctgct attagtgata ttgctcatcc acaatggaga gcaacatcat
      781 taggagttta tagattctgg agagatttag ggtttgtaat aggtgcttta ggtataggct
      841 ttatcgcaga tttctttagt ataagcatgg caattcaaat agcaggatgg atagcattag
      901 gttctggaat ttttgttttt atagttatgc atgaaactaa aaggaattaa caataagact
      961 tttatattta agcaactttc tatagccatt cttatttagg aaatccaaaa aattttataa
     1021 ttatattaaa aaatttgaaa cataaaagat tatagcataa gtgatagaaa ataataatta
     1081 atcatttatc atatatcatt gcaatcaatt aaatatttta tgttcctata tataagtagg
     1141 caaaaatagt gttttatgta agaaaaaaat caattaaaac gatccaatac cttcctagtt
     1201 agttggtaca ttctctccca tctctgtata caccatcatc tgaaaacata ttaattttaa
     1261 ctattaatat tttaataact acaataacaa tttcaaaaac aagtgaagca actgaaaata
     1321 caattacaag tacaagtaca agaataagaa tagtgttaaa gaataagatg ggtgttaaat
     1381 ttgccgcata acacccatat tttacatctt cttcaatgaa tagagatagt agatgcaata
     1441 atatatatac aatataaaat aaaaggattg ttgttgattt ttgagtgtta aattattagt
     1501 tttttcagca gaggaatcat atcaagattt atttggattt gctattatta ctataacata
     1561 accatgcaag aaaaagttgc ctgccctatt tgtaaactcc ttgggtttaa aagtctcgtc
     1621 tatgagattg attggaaaag ccattttcaa aaatttggag gatattatga tgaaaatggt
     1681 aactatatag aatctgatat tgttccttct cctctagtaa gtaaaacttt ttggtgcacc
     1741 tatggtcatt cttggataca aacttaatat aattattttt ccagagaaag taaaaggaat
     1801 gcatttggct atttcccttt cacttttccg gacttgacat ttggagcgat tggtaaatcc
     1861 cactataaca aatctgagtt ataaataatt ataatagata ttctattgat ctataggaaa
     1921 tttatagacc aagttttatt ttacttttaa gtttacttat tttaatctat taaaaagtaa
     1981 tatcttcgtt ttcatcatta ttattttttg ttgataaaga atatttcttt tggtattcgg
     2041 gatcttccca taaattgcga tatctatcat aatattgagt cagttcaagc aaaactattg
     2101 gtatagtcaa agcaagtcca ataacgatga gtaattttgg atgtaaaatt tctttttact
     2161 tttattatga ttatgttctg tttgtccata ttcttcttcc tcttctcctg cttctgttac
     2221 tggtgttgct gacgtattta ataaaaagaa atagacaaag cgtaaaggaa aagaaattaa
     2281 agtagaataa taataagaaa ttttttaact tatttttatt ttattatttt aataattttc
     2341 ataatttact aaattagata aattttggac aacaaaaata agaaaacgtt taatacttga
     2401 ttatattcaa ctaaaacttt ttttaaaaaa gtatttatga atttgaatct ttttaaacaa
     2461 ttgagaattc agcctataaa agtagcaaat tttgctttag aaggccaagg ctggaagccg
     2521 atgcttgaaa gtaattattc ttcttctacc tcttcattag atgttgtaga agttattttg
     2581 aaagcagtta aatcaaacaa tccagaaatt cgatacttag taggtaatga tgcactattt
     2641 ctcattgaaa aaagacaaaa tataacagat aaagaatttg agaactggat gaaagagagt
     2701 ctgttcgaac aaaaaggatt tgtagttgaa tggaaggcta gagtacaagc ctttctttca
     2761 agtattaact ggtacaatat atagttcgaa ataaccatcg agacccaata atcttttcat
     2821 tatttattta gttttcttgt tcgtaacttg cttgtcaata attaattgta gtatttcctt
     2881 agacatgatt gttttttgaa tcaataattt tataaaagct taaatagaaa attatattat
     2941 aaatagatga aaaccagact tgatctgtat ttttcagcaa ttatgattac tcgaacaaca
     3001 ttcggcatta gcttctcatt ctttttaaat aataccaatc cattagtata tggcatggaa
     3061 acctacaact attctactgt agaaattaat actaaattat ttttagaaaa actccaacaa
     3121 agtggtggtc ctccaatcta cactttgacg ccaagtgagg ctcgtaatgt actatctgga
     3181 cttcaggcag gcccgataga agaacttcct gctgaaatag agaacaaaac tattccaggc
     3241 ggtcctaatg gtgaaatatc tattcagata ttaaaacctc caggcaccgg caatgaaact
     3301 cttcctgttg taatgtactt ccatggaggc ggatgggttc taggtggttt tgatactcat
     3361 gaacgattgt taagagaact tgctaacggt gcccatgctg caattgtatt tgtcaactat
     3421 actcgctctc ctgaagcaaa atatccaata ccaatagaag aagcatatgc tgctacaaaa
     3481 tggatttcag agaacggtca aaccctcaat ttagattctt cacgtttagt agtagcaggt
     3541 gatagtgtag gtggcaatat ggctactgta gttgcattac ttgccaaaga gcgtggtgga
     3601 cctcctatca tcttccaatt actcttttat ccagttacag atgcaaattt tgatactcca
     3661 tcatatttga aatatcagga aggttactgg cttacccgtg atgcgatgaa atggttttgg
     3721 gataattatc tttcaaatga tactaataga aaagaaccaa ctgtctctcc actgcaagca
     3781 tcaattgaac agcttagtgg attaccgcct gcacttatca tagtagatga aaatgatgta
     3841 ctccgtgatg aaggtgaagc ctatgctcat aaattgatgc aggctggcgt tcctactaca
     3901 gcaactcgat atcttggtac tatacatgat tttgtaatgt taaatgtact taaagatact
     3961 ccagcatcaa ggggtgcaac agaccaagca tcacagacac ttaaagaaat atattcaaaa
     4021 tagaaatatt caaagtagag gatagccatc tattttattt attaaaacaa tatttatgaa
     4081 tataatatag ttgagtatat tcaattcaat attattctaa atgatgcaag aatgatagga
     4141 agaagatgtt gcgtaattag tctctcctta cttgtctgta tgtaaatttt tctattctaa
     4201 ttaggaaact taaacagcta aaaaatatag atttaagata ttccaaaagt aggtatagca
     4261 gctcctgtta tagtctttgc ttcctctgag ctgagaaata atattacatt tgccaaatcc
     4321 tctggcttta cccatttatt aaaatctgca tttggcatcg cttttcggtt tgcttcagta
     4381 tctattatag ttggcattat gcaatttaca ttaatatttg attctttggt ttcattagat
     4441 agtgattcta caagtcttat tagtccagat ttggatgctg cataagcaga atcataaccc
     4501 tcagaatgta agccaatttt ggaagagata tgtattattt ttccatattt tgaagagatc
     4561 atttgtttag cagtatgttt acttatcaaa aatgctgatt ttagatttac tgtcatcatc
     4621 atgtcccatt ctttttcttc aagatcaact atggccttcc ctcctagata agctccaacc
     4681 acattaacca atatgtcaat acgtccatat ttgtctaata tattagaaac taacttttct
     4741 acctcttctt catttgttac atttgctttg aatagttgta ctgtcggttt gttctctgtt
     4801 tgtatttgac tagtttcttt gtcactgtga tatgaagata ttaccgtagc attggaccca
     4861 aggaaagcct ttgtaataga tcttcccata gctcctgtac cacctgttac tagtactatt
     4921 ttatttgaaa agtcatatct attactatta ttatgattag ccataggatg taatatttaa
     4981 tgtcatacta agtaaaaaaa gattattggt acttttacaa taaacctatc taaggcatta
     5041 atgtacctac tttgacaaaa tagtttttag aaataatgta tcaatagttg aattagaaac
     5101 taataaaggt gtacaaaaat acgctttatc gaatcagcct gatatcaata ggatatttat
     5161 agatatatca ttctacatag tgctcatgaa ttaactgatc ataaagtcta atagatttaa
     5221 tatttacaaa ttctagcatt ccatatcttg ataactccct accaaaaccg ctctttttta
     5281 caccaccaaa tggaactctc ggatctgaaa ctactacatt attgacagat actatacctg
     5341 attttatgat actagatagt acttctgcat tttcaaggtc ttgtgtccaa atactggctc
     5401 caagaccgaa ttgagagtta tttgcaagtt ttatagcttc tttttcatta tcagctataa
     5461 gaataggagc agccggtccg aatacttcct catttactat acgcatattg ggagtaatat
     5521 tgccaagaat ggtcggcttg tagaaataac ctttacttcc tattttttct ccacctgtaa
     5581 ggacttccgc tccttcattg atagagtcgt taacttgtga atctatagtc tctaatgcct
     5641 ttgcatttac aagtggtcct atatccgtat cttcagaaga gggatcgcca actttaagtt
     5701 tctctgtttt ttgaataaac ttttcaataa acacgtttgc aatattcttt actacaatga
     5761 atctttttga tgcaatacaa ctttggccac aattaataaa acggcctttt actgctcctg
     5821 ttgatgcttt ctctaggtct gcgtctgcac atacaataaa tgggtcactg cctcccaatt
     5881 ccagtacagt cttttttagt tgagaagttg ctctttgtgc aacttttgca cctacaggaa
     5941 cgcttccagt aaatgtaaca gcatttacat ctggagaatc tacaagtatt tctgcaacag
     6001 tagagtctcc tattagtgta ttgaagatcc catcaaataa tccagctttt ttaaaaatat
     6061 tttcaatttc tattccgcat tgcattgtag cacttgctgg ttttaataca attgtatttc
     6121 caactactaa tgaaggagcc gcaaatctta atgcctgcca atatggaaaa ttccaaggca
     6181 ttatacttcc aataactcca agtgcttcga attttatagt gctctttctt gcatcagtat
     6241 taaaagattc atcgtgaata aagttctttc cgttatctgc aaaatattca atagcccaca
     6301 cacatttatc aacctccgcc cttgcttctt ttattgcttt acccatttca tttgtagctg
     6361 ttctagaaag attttctttg ctctttctta attctttaga aacatcatat agcgaatcaa
     6421 tccgtttgtc caaatcttta tcccattctt tataggcatc ttgggctttc tttacaatgg
     6481 tatttacctc ttcttttgtc attatggtat atctgttgag aatttcttct gttgacggat
     6541 ttatcgttgt taattcagag tttgtcatgg atatattaca cttctaccaa gaattttacc
     6601 gtcctttaac atcctcaatg cttttgtggc ctcttcaagt ctgaatctat tagaaataac
     6661 tgattttatt atatttctct ttactaatga aacaagctct attaaatcac tcatatttcc
     6721 agtatatttg ttatagtagt gtactaacac taaagtagta ttaaactatg tacagtaata
     6781 ctaaactttg aaaagacgat ggccataaag atttgaggaa aaatttgtta tcaatcaagc
     6841 cagcttctgt tcaactctat ttttctggta ctggaaaatt tttaatatat tgttgaatgc
     6901 tcaattatga ttttctttat cttgcaatga aaatattaca tctttcctcc attgattttt
     6961 gattgattaa atcttctagt caaagaatat cattaatctt ctaaattaat attttagagt
     7021 tatttgtgtt gatttaacag aaatataaat aatgaatatg cgttggattt ctcttcttgt
     7081 aagttatcat catttctttc atcttatttt cacaagtaaa tcgtattaga gaattaatca
     7141 caattgagcc agtgggatat gaaacaaatt tgaactaagt aggatttgtt tcattgaaat
     7201 atggaattta tgatattaga caaattatta tttcatttat atcaaataag attttcgaaa
     7261 tacataaaag tttgagtata cacttcatta tgtaagatga ataataatga cttttcaaat
     7321 tatttgatgg atataattaa caaaggttct ttatctatta tgttatctat aggtcataga
     7381 acaaaattat ttgatatatt atctatcctt ccaccttcaa cagttgaaga gatagcatca
     7441 aagtcaaaac taaatgaaag atacataaga gaatggcttg gagcgatggt aactggaaaa
     7501 atcattgagt atgattcaat taataacaaa ttttggcttt caaaagaaaa ggctcaatat
     7561 ttgactagag aaaataacat atataatttt gccgcatcta tgcaatggat tcccatatta
     7621 tctcaagtag aagatgaaat tgttgaatgc tttgtaaaag gtggcggggt tccatattct
     7681 tcatatagtc gatttcatga agttatggct gaggaaagtt atcaaactgt tgtaatagga
     7741 ctaattaata atatcttacc tttagttccc aatcttattg ctgatcttag aaatggaatt
     7801 agagttcttg atataggatg tggtaaagga aaagccgtta atttgatggg aaagcatttt
     7861 ccaaaaagtg tcttttacgg gtacgatctc tcaaatgaag ctattgatga tgccacaata
     7921 gaagcaaaag atatgaacaa ttcaaatgtc ttttttaaga ttcaggatat tttgaattta
     7981 acatttaacg ataaatttga tttaattact gcatttgatg caatacatga tcagcccaaa
     8041 cctgatttgg ttttaaaaaa tatctataat tctctctctg ctaaaggagg agtctttctt
     8101 atgcaggata ttcttgcgtc aactccatta aaagataata tttcacatcc gttgggaaca
     8161 ttcctttata ccatttcttg tcttcattgt atgagtgtgt cattatccca aaatggtgct
     8221 ggtctaggtg ctatgtgggg aaaagagaaa gcagtcttta tgttaaaaga agcaggcttt
     8281 gccaatgtag aagtaaaaac attaccacat gattttcaaa actattatta tatttgtcag
     8341 aggaacaaat aatataatat agtcatgaga tattcaataa aggaaatcca attatttcaa
     8401 aagtcaatca ttgatttgtg aaccaaagtt gacataaaaa gaggagttga actacaaata
     8461 catattcatt aaaattcagt ttcattggtt atagtggatt atttatttat ttattattta
     8521 ttaatttatt aaataaaggt gatggatatt acaatgttta taacagagta gacaatatga
     8581 taaataaaat ggactcatct tcatcaaaaa tacagaaaaa gaatttagac tcttcacctg
     8641 atgatactag aaaatttgaa aaaggaaagg ttgagattgt aaactttggt aatatgacaa
     8701 taggacgtgc aatttttgaa cctggttgga gttggagtaa gtgtattaaa ccaatagtaa
     8761 atactaatag ttgccaagcc ccacatactc aatatgttat ttcaggacga ttaaaggtag
     8821 ttatggatga tggaacagag gaagaatttg gaccaggaga tgctgctatt attcctccag
     8881 ggcatgatgc gtgggttgtc ggaaatgaat cagttgttgc aattgatttt actggattaa
     8941 aggactatgc aaaaaaagaa taactaattt cacattaatt catcaattat ctctgtactt
     9001 atccaagtat tagacagctc attagcaaca aatactctat cttgttcttg tattgaatct
     9061 tgagcaaaag aatattgagc tagttcgctt agaactaatt attattaaca aattaataat
     9121 actaccaaac gttattaaaa atgatgattc taagaatgtt ttatctttgt atgttcagat
     9181 ggatatagta gtttttaact attccgtaat aatggttttt taaattccta tttttttcga
     9241 tttggttaaa tattaaacaa attagaatcc aaatttgtac atctgttttt aaaagttttt
     9301 cattagtttc aaattcaata tagtcaaaaa atctccttta tctcctttca tgatattttc
     9361 ttgttaaatc tgttttaatg tatatttgaa aattaaatat ggagttaaat ctttattatg
     9421 ttctattttt gatacaattt caattcaaga agattcatat gctgcaataa aaaaattgta
     9481 ttgagattga gcttgatagt caattagaat ttttacgtta ggattagtgc tatattgttc
     9541 ttcatgatga gtatatgaaa tggattggaa aatccatata tcatctcaag aaatttttct
     9601 atttctattt tgggtatctt taagttttca attatataac gacataaaat agatgctttt
     9661 atcgtattat ttgggaataa tttaggagtc cttcattgtg attttcttta aaaaaggctt
     9721 tgctaagtat attttcttga aaaattcaaa taatgtttat ttgtactatc ataactttat
     9781 aaaattgttt gaatcatatg atacatcagg gtataaacaa acaaaaccca ttgaagacat
     9841 gctgttaata ataaaattac aaaagatgga aaaggataga aattaaatat aataaatgtt
     9901 catgtttttt ctttgtttat acctttttcc tttcaaatag aaattgattt tctgttgata
     9961 catgattaac ctttagtcgg atcttctgtt aattataaaa aaaatgatct ctaaaaaaaa
    10021 tttatagtat ctctgcactc tgctttttta tagaatcttt ttttattcat aaaatcttta
    10081 cgctcttatg gatgattaaa ctagattttt gatagctctg caagttggaa atacaaagta
    10141 aagtgaagat attaatccat tgtagaaaaa aaataagaaa ataacaggaa aaagaccttg
    10201 cgcatttatt agtgatggag ccaaaacttt catcaaattt acaagaaaga atttttcact
    10261 ataaataacc ctagaaggac atattagcca tattcgattt gaaggagatt ttaacaataa
    10321 caaaatggaa aggatgaatg gcgaaattag aaatagagaa aaaacaatga gaggattgaa
    10381 gaaatgtgat tctcctagtt taaaattaaa ttagtatctt tcaaatctta aactaaaagt
    10441 taatagatta gaacaaaaaa ggaagaattt gcaattttat ttttattcga acagttatag
    10501 tttacggaca ttctggtaat aaatgaatac tcttttgtag aatattatta taattaaggt
    10561 gataaattaa aattttgata ttattcttat actagtagga gaaaaaaata gaaatatgtc
    10621 tatactttca aaaactctta cattacgaat tccttccaat ataatttacc gcgctttaaa
    10681 ggatacacga ttagaaaaat tctttcctga attttttata ggcatagaaa gaaaagttat
    10741 aattgataaa ataaataagg agattagttt taatacaata actcaaggaa gacagcttga
    10801 aattattgaa acattcaaat ttaaaatttc tagcaataac gaaacagttg taaaatatat
    10861 tacagagaca aaaagtatag aagggaacaa tatagtatta gaatcaatag tacagacaca
    10921 tgtagctaat attttatatt cacttttgat gcttgagaca ggttatatca atggagtatt
    10981 agaaaagaaa aaaacagctg attcaatatg aatatcaaaa aaatcatcgt ttgctttaaa
    11041 ttaccattat atgagaaagc ttattataaa atatatttaa aaaatattga ttaagtacca
    11101 ttactaagaa ataactaaca aaaaaagagg ctaatgataa accataggga atcaattttg
    11161 taataatttt catttataaa atatcttggg acacttctaa caaaattttt ataattttat
    11221 aaaatatata tataataact tgaaacatac tattatttag ataaaaaacc atagccacct
    11281 ctggatataa taatagagga ctatggctaa aagagaatat ttcttattga tagtctttga
    11341 agtattttca aggttaaaaa aataataatg taatatataa atattattat caccaaaaaa
    11401 gacatataat ggcgatcaac aaggagacaa agagcctagg tatgccttgg gagaaagagt
    11461 acggttactc ccaggcggtg aaagttcgag acactatcta tctatcggga caggtaagtc
    11521 atgatgacga gggcaatata gtcggtctca aagatatgga gactcagatg cggcaggcat
    11581 atgccaatat taagaaggtg ctcgcacagt atggcgcaac aatggacaac atagtagatg
    11641 aagtactctt cgtaacggat atgaaatcag ccttcgcagc agcggtcaag tgtagacgag
    11701 agatcttttc cagcactcct attgtggcaa gcaccattgt gcaaattaat ggtcttgcgt
    11761 ttcctgagtt catgatcgag attaagtgtg tagccaaatt ggaaacctct ttgtaaagta
    11821 aaaggtgggt atgaataagt cgagtattat taatggctgg tatatgtctc ttttaatagt
    11881 agcgatagta ccataataaa agatagaaac aaagagcaaa aaggcaaaca ggtacaatat
    11941 atgaatcaga aataaataac tgtgtttggt tctactctat taggttgcaa caaaaattaa
    12001 tagttccttt cattatttat tataaatgat taataaaata attataacat ctactttaac
    12061 aatactttga ttttcatctt atcttatgta gatttcggat ttatggtttc gttatcttat
    12121 taatgaataa taataataat aataataata ataataatac agataaggat cctataaatt
    12181 aagttcctag aggacaatag ctttataatt tgaaattaat cttctttctt taaaaattaa
    12241 ctagtctcac tttagaattg tttaaatctc accatccata ttcgacaaga ggtctgttaa
    12301 catctttaat tgattatcaa aaagtatcag ataagttcca tttttttttc tgaaatttag
    12361 attggaatat gaatttaaat attagaatta tatacattca acaatgataa ataatctatt
    12421 aactatactt gtagtagcag caagcttaag tgtattgact ctaaatagta tatccataat
    12481 aggagtaaca gaatcttatt cacaaacagc tgaaacacag gcaaaatcac aatcatctaa
    12541 tacaattcac ataacaaaag atgcttcaaa ttcttatgta ttatcttcag gttcatccca
    12601 gattgggaca tttgatacta catatactat agcaggaagc gttacctcac ttaaagagtc
    12661 aaatgatttg gtaacatcaa caatcatcaa ggactttgat aaatccccag ttataggata
    12721 tgttatagct catacaagta aatcaaactc taaattgcca actttagcta atccatttgt
    12781 agacaaaaca acaataaatg aaaaaatcaa aagtgaaatt caaagttcta taggttcatc
    12841 tcaaaaatta aaaactacgc aagcagaaat aaagtgtaat tttggcatgg aaataaaaga
    12901 ctggaaatgt agtacacatg gtctattagg ctaacaacta taattaattt tttttaggtt
    12961 tcaaattcta aatctattaa ataattctat taaataattt ctacgaaaat aattctaatg
    13021 ttcctatatt ttcctttaaa ttgtcacaac atcttattaa ttattcttat tggaatagaa
    13081 ggaatatcta tgacaaacaa aattttaaat aaaataataa tcaacagtct atgttaaaaa
    13141 ttaaaccaat aactcttgag ggccatcaaa caatcttacg tcctccttct cttaaagatg
    13201 taaaaggtct atctcttgct gcccaagatg gggaaatctg gaatactcgc tttgcttact
    13261 ttccccatcc aaatgaaatg tcaaaatacc ttaaagagtt attaaatggt tcctcacctg
    13321 gtaaattttt accatttata actgtacaca aattatctaa tacaattgta ggtactactc
    13381 gttatctcaa tattgattgt gaaaaccatc gacttgaaat aggacataca tggattgcta
    13441 aatcttggcg tagaacgtca attaatactg aagctaaata tttgatgttg gaatacgcgt
    13501 ttgaaaaact aggatgtata gctgtagaaa tacgtaccga tgtccttaac atagtttcac
    13561 gtaaagcaat tcaaaggtta ggcgctaaac aagatggtat actacgttat cataagataa
    13621 tgcacgatgg aagaattaga gatacagttt gttatagtat catagaacct gaatggaaga
    13681 gtatcaaaaa aaaactgatt gaaaagatgc atcagtataa tgagtaataa aaaattaaga
    13741 tgatacattt ctattttttt agatataaaa aataaaaact atttcaaaat aaaataaaaa
    13801 ttgattaggt acaataattc aaaaattcac gttgtatact aggttaaaat taaaacctag
    13861 ttggttttta gttgatatta ttattattat tattattgtt gttgttgttg ttgttagtat
    13921 ttacgtgaat tttgtctatt aactgattgt atgaaattgg cgtttgtttt tgcgcaagcc
    13981 tttagattat cacgtgtttg ttctagtgct gtattgtata ctctcatatt ggatgctaga
    14041 gcattgttta ttaaattgct attatctaaa aggtaacttg taaaatgatt taccattact
    14101 gcgtgatttt ctgcgattct ttgtgaatta tacaaatccc atacaccact atttctgttt
    14161 acttgtgatt gaaaagagcc tattacttgt ttttctgttt caataaagtc agaagcaata
    14221 tcttttatag tttgaatagt ttgttcttga tattcagcaa cacgttgtgt atatcttgga
    14281 atttctcgtg cagcatcatc agtagtcctt tttacattct gtaaggtgtt gtctagtgtc
    14341 ttgtttattg catcttgttg ttgatttaag gtgttattgt agtctctatt tgttctttca
    14401 ttgaaattgg tggtattagt gttgtctgtg tttaatttag ttctatttat gtt
//
LOCUS       DASVEV010000220         7682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1264007, whole genome shotgun sequence.
ACCESSION   DASVEV010000220 DASVEV010000000
VERSION     DASVEV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264007"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VF222_09825"
     CDS             <1..377
                     /locus_tag="VF222_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142763.1"
                     /translation="SLYSLWNSIGVLKNQGTAVLLAENSHGIGEGALTMYLEDRLNLS
                     EIKKINYVNGLEHLVYLENLKNEYYIGIVSSIPFHYTKTKLGFNTFNNVKEVLQNMIS
                     RYGKSSKVLISPDPNITLFMKQ"
     gene            485..982
                     /locus_tag="VF222_09830"
     CDS             485..982
                     /locus_tag="VF222_09830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7142764.1"
                     /translation="MQSILRSVGGYVIRNCEEKDLPFVININMITLPEHYSDYFFTSI
                     LAELPEAFIVAEIKNQIVGYIMCKIEFGFSNFRKLGFVKKGHVVSVAVLEEHQGKGVG
                     TALMLEGINGVIIRKSDEIYLEVRVSNESAVQLYKKLGFIIKSRLKSYYRDGEDAFLM
                     AKDLS"
     gene            1018..1236
                     /locus_tag="VF222_09835"
     CDS             1018..1236
                     /locus_tag="VF222_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142765.1"
                     /translation="MKNNRPTGIFLLIVPPLFFLIYIYLLFASRFDLLLIKFTIVGTV
                     GIIVSVFIWIGYTMISTSNPNEITDDNI"
     gene            complement(1233..2075)
                     /locus_tag="VF222_09840"
     CDS             complement(1233..2075)
                     /locus_tag="VF222_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX7142766.1"
                     /translation="MGSMLKNALKDVFAKEEIERVYSSFDIIGDIIIIKIPEELEEKK
                     EIIAKTLINKIKTVKTVFQQISSVQGEYRTRRLCFLTGINKTSTEYREHGCIFKVDVL
                     KTYFSPRLATERLRVSKLIGNNEIIINMFAGIGTYSIILAKKNPDCIVYSIDSNPYAN
                     DLCIINSKLNKVQDRVIPILGNARTIISNQLKGKATRVLMPLPEQAAEFIDSAIMALE
                     NNKGIIHYFAHIKAKSKLDALEEGKVNCHNHFQCYQYKIISGKVVREVGPRIYQTVFD
                     LEIF"
     gene            complement(2080..3882)
                     /locus_tag="VF222_09845"
     CDS             complement(2080..3882)
                     /locus_tag="VF222_09845"
                     /inference="COORDINATES: protein motif:HMM:NF009945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HEX7142767.1"
                     /translation="MGNPESTARVAVLDEELCKPKKCGLECIIYCPVNKTGGKCIVQR
                     EEDAKAVISEDLCTGCGICVKKCPFEAIVIVNLPKELGEHKIHQYGINSFRLYHLPTP
                     KKGSVVGLLGRNGMGKSTIVNILSGNLKPNLGNYDLNIEWEYVLEKFHGSELKSHFES
                     IANGKFRVSIKPQLVYKIPKVFKGTAGEILKKFDETNTADEISEKLGLKNSLNKNLNE
                     LSGGELQRLAVTVTAIKDADYYFFDEPSSYNDIYQRLAVAKVIQNLATKGKTVMVIEH
                     DLTLLDYLSDYIHILYGESGAYGIVSSLQSTKTGINNFLDGFIPSENIRFREKKFRFD
                     TFNPVDDFILNNIVTQYSELEKKFNDFRLKISQGKIRKGEVLGVIGANALGKTTFMKM
                     IAGVEQADNGKIETNAKISYKPQYISQEYDSDVKSLLYTAYQNIIEGSIAEEQIIIPM
                     GVKKLYEKNIRNLSGGELQKVAIALSLMRDADIYALDEPSAFLDIEERIALAKFIQRF
                     VRAQGKSAIIIDHDMQLIDIVSDSLIIFNGKPGIDGIASKPISKIEGMNQFLKDLSIT
                     YRRDESTGRPRVNKNGSQLDKLQKNSGEYYYIKR"
     gene            3998..4116
                     /gene="rrf"
                     /locus_tag="VF222_09850"
     rRNA            3998..4116
                     /gene="rrf"
                     /locus_tag="VF222_09850"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4152..5624)
                     /locus_tag="VF222_09855"
     CDS             complement(4152..5624)
                     /locus_tag="VF222_09855"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7142768.1"
                     /translation="MLKIIADETKISEKRTELLVIGCFLNEDYSNLLNKFNEEISIAG
                     KEIISNIHKFGLLNVLHTFAKLPARKILFIGLGNKNEFNTEKSRIISGKIIQYAKEFK
                     IKELSIIPFNFSKEIIESLVEGIILSSYSFEKFKTSMNNNTIDNVSILIESNSKEYQK
                     IINEISVICQSVNFGREISNLPPNECSPEYLAKIASDIKNQGIKSKIISLDSMKEMGL
                     NGIVSVGGGSINTPKLIVLKYEGTKNEENPILLVGKAVTFDSGGISIKPSEKMEEMKF
                     DKCGGVNIIAIMKSLSSLKIKHNVIGIIPAVENMPSNSSYRPSDIIHMYNGKSVEVIN
                     TDAEGRLILADALAFGIKHYKPKLIIDMATLTGACIIALGSNTAGIIGNDEKLIKKIL
                     DISKVTHEKMWELPLLEDHHDQIKSKNADIKNVGGRAAGAITAAAFLSNFVENIPWVH
                     LDIAGTAWNQEGSLLKPYNPPGATGFGIRTIIKFIMSESV"
     gene            5772..7409
                     /gene="rpe"
                     /locus_tag="VF222_09860"
     CDS             5772..7409
                     /gene="rpe"
                     /locus_tag="VF222_09860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7142769.1"
                     /translation="MIFHMRYLDLKRKIQEIRINAIEGIAEAGSGHPGASFSAAEIMG
                     VLYFRELRHDPSNPSWDGRDYFINSKGHSAPGYYATLAVAGYFPKHEMKDLRKFGSRL
                     QGHPVRYSKLLEHHSVPGVEYSGGSEGIGLSVSIGIALANVIDNKDNNVYVLLGDGET
                     NEGQVWEAAMTAPKFKLDNIVAILDRNKIQQDGFTEDIMPLDPVRDKWVAFNWNAIEI
                     NGHKVEQIMDALNKASEIKDKPTIIIANTVKGNGIKHMANNPQWHGKAPHNMHIPLLI
                     EELEGEYMIAPSIIAGEKENYEEKIKKVERGGADIIHLDVMDGIFVPNITFLADTIKK
                     LRPITNIPFDAHLMIEKPYEHIEEYINAGCDIITIHAETCDNEKFCYIREKVMSAGIS
                     LGIAINPQTELPEWAFSYLNDIDVIIVMSVNPGFSGQKFIPDITSKISNLNITLKMKG
                     YKGYIEADGGIDASTVQRVYEAGARIMVAGSAVFSNSDINTAILQLKHKTNVTLERNL
                     LKKADENSIRKDWIIARKHILIPVANELGIEDEINRL"
     gene            7390..>7682
                     /locus_tag="VF222_09865"
     CDS             7390..>7682
                     /locus_tag="VF222_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142770.1"
                     /translation="MKLTDFELRKGDMRSAYGNALVELGTINKDIVCVGADTTDSLKT
                     KKFGDQFPERFFNIGIAEANLVSIAAGFAIAGKTAFASTYAAFLPGRCLDQIR"
BASE COUNT         2491 a         1187 c         1185 g         2819 t
ORIGIN      
        1 actcattata ttcattatgg aattctatag gagttttgaa aaatcaagga acggctgttt
       61 tactagcaga aaatagccat ggtataggtg agggtgcttt aactatgtat ttagaagata
      121 gattgaatct ttctgaaatc aaaaaaatca attatgtaaa tggattagag catttagttt
      181 atcttgagaa tttaaaaaat gagtattaca ttggcatagt ttcttcgata ccgtttcatt
      241 atactaaaac aaaattagga tttaacactt ttaataatgt taaagaggtt ttacaaaata
      301 tgatatcgcg ttatggaaaa tcaagtaagg ttctgatttc tcctgatcct aatattacat
      361 tatttatgaa acaataagaa ttacttttaa tatctgagac aacttttatc tatttatttt
      421 aaaatacttt acgaatttgt tcttgtatat aaagttaaat accatgtcaa attaaatata
      481 gaaattgcaa tctattctta gaagcgttgg tggttatgtt attcgtaatt gcgaagaaaa
      541 agatcttcca tttgttatta atataaatat gataacactt ccagaacatt attctgatta
      601 ttttttcact tctattttag cagaattacc tgaagcattt attgtagcag agataaaaaa
      661 tcagatagtt ggatatatta tgtgtaagat agaatttgga ttctcaaatt tcagaaagtt
      721 aggttttgta aagaagggcc atgtggtatc agttgcagtt ttagaagagc atcaaggaaa
      781 aggtgtagga acagcattaa tgttagaagg aataaacggt gttattatta gaaaaagtga
      841 tgaaatctat ttggaagtta gagttagcaa tgaaagtgca gttcaactat acaaaaaact
      901 aggttttatt ataaaatcta gattaaaatc gtactatcgt gatggtgaag acgcattttt
      961 aatggccaaa gatcttagtt agagatatat gatatttaaa ttggttttct tatgataatg
     1021 aaaaataaca gacctactgg gatttttctt ttaatagttc ctccactatt ttttttaata
     1081 tatatatatc tattatttgc ttcacgtttc gatttgctat taataaaatt taccattgta
     1141 ggaactgtag ggataattgt ttcggtattt atatggattg gatatactat gatttctact
     1201 tctaatccta atgaaattac tgatgataat atttaaaata tctctaaatc aaaaacagtt
     1261 tgatatattc taggaccaac ctctcttacc acttttccag aaattatctt atattggtaa
     1321 cattggaaat gattatggca atttactttt ccttcttcga gtgcatccaa tttacttttg
     1381 gctttaatat gtgcaaaata gtgaataatt cctttattat tttcaagagc cataatcgct
     1441 gaatcaataa attctgctgc ttgctctggg agtggcataa gaactcttgt agctttacct
     1501 tttaattgat tagatataat tgttctagca ttacctaaaa taggaataac cctatcttga
     1561 actttgttta atttggaatt gataatacat aaatcgtttg cataaggatt tgaatctatg
     1621 ctatatacaa tgcaatcagg attttttttt gcaagaataa ttgaataagt tcctattcct
     1681 gcaaacatgt taatgattat ttcattatta cctatcaact tagaaaccct aagtctttca
     1741 gtagcaagac gaggagaaaa atatgtcttt aatacatcta ctttaaatat acatccatgt
     1801 tctctatatt cagtactcgt tttattgatc ccagttaaaa aacataatct tcttgttctg
     1861 tattctcctt gaactgaaga gatttgttga aaaacagttt ttactgtctt aattttattt
     1921 atcaatgttt tggctataat ttcctttttc tcttctaatt cctctggaat tttaattatt
     1981 attatatcac caattatatc aaatgatgag taaacccttt caatttcttc ttttgcaaat
     2041 acatctttta atgcattttt aagcattgat cccatgtctt tatcttttta tgtagtaata
     2101 ttctccagaa tttttttgta atttatctaa ttgacttccg tttttattta ctcttggtct
     2161 accagtactc tcatcccttc tgtaagtaat agataggtct ttaagaaatt ggttcatacc
     2221 ttctatctta gaaatgggtt tgctcgctat accgtctatt cctggtttcc cattaaatat
     2281 tattagagaa tcagaaacta tatcaattaa ctgcatatca tggtcaataa ttattgctga
     2341 ttttccttga gctcttacaa acctctgaat aaattttgct aatgctattc tctcttctat
     2401 atctagaaaa gctgaaggtt catctaaggc atagatatca gcatctctca tcaatgatag
     2461 tgctattgct actttttgta attctcctcc actaagattc cttatatttt tttcatacag
     2521 ttttttaact cccattggaa taataatttg ttcctctgca atacttcctt ctataatatt
     2581 ttgatatgct gtgtataata atgacttaac atcagaatca tattcctgag atatatattg
     2641 tggtttatat gaaattttag cattggtttc aatctttcca ttatctgctt gttctactcc
     2701 tgcaatcatc ttcataaaag ttgtttttcc taatgcattt gcaccaataa ctcctaaaac
     2761 ttcacctttt cttattttcc cctgggagat tttgagacga aaatcattaa attttttttc
     2821 taattctgaa tattgagtga caatattgtt taagataaaa tcatcaacag gattgaatgt
     2881 atcaaacctg aacttttttt ctctaaatct aatattttct gaggggataa aaccgtctag
     2941 aaaattattt attcctgttt tcgtagactg taaactggaa acaattccat aagctcccga
     3001 ttcaccatat agtatatgaa tatagtcaga caaataatct aataaagtta aatcatgctc
     3061 gataaccata actgtttttc cttttgtagc aagattttgt attactttag ctactgctaa
     3121 tctttgataa atatcattat atgatgatgg ctcatcaaag aaatagtaat ccgcatcttt
     3181 aattgcagta acagtgacgg ctaatctctg taattctcct ccacttaatt catttaagtt
     3241 tttattcagg gaatttttca aacctagttt ctcagaaatt tcatctgcgg tattagtttc
     3301 atcaaatttc tttaaaattt cacctgcagt tcctttaaat accttaggta ttttataaac
     3361 aagctgaggt tttatggata ccctaaattt cccattagca atactttcaa aatgtgactt
     3421 taattcagaa ccatgaaatt tttcgagaac gtactcccat tcaatgttta aatcataatt
     3481 tcctaaattc ggtttaagat tgcctgataa tatatttaca atagtagatt tgcccatacc
     3541 atttctacca agcaaaccga caacagaacc tttctttgga gtcggtaaat ggtatagtct
     3601 aaaagaattt attccatatt gatgaatttt atgttcaccc aattcttttg gtaaatttac
     3661 tattacaatt gcttcaaagg gacatttttt aacacatatt ccacaaccgg tacatagatc
     3721 ttcggaaatc actgctttag catcttcttc cctttgaaca atgcatttac cccctgtttt
     3781 atttactgga caataaataa tacattctaa tccgcatttt tttggtttac ataattcttc
     3841 gtctaaaact gctactcttg cggtggactc agggtttccc atttagttat atttttaccg
     3901 atatgtatta tatctcttgc tagggaggat ttttgattgt tgttacttgt atgtctaggt
     3961 ttaaaaacga ataaattaac cttattataa tttttattta gccggtcata gtggcagggc
     4021 gcgacccgga ctcataccga acccggaagt caaacctgtc gtcgctgcct agtactgacc
     4081 tacgtgagtg gttgggaaag ggcagtgctg gcgtccaaag ttataattta tattaaagga
     4141 tgttatatat tttatacact ttcactcata ataaacttga ttattgttcg tattccaaaa
     4201 cctgttgctc cgggtgggtt atatggtttt aacaaacttc cttcttgatt ccatgctgtt
     4261 cccgcgatat cgagatgtac ccatggtata ttttctacaa aatttgacaa aaaagctgcc
     4321 gcagtaatgg ctcccgccgc tcgaccccca acatttttga tatccgcatt tttacttttt
     4381 atctgatcat gatgatcttc taacaagggt aattcccaca ttttttcatg agtaacttta
     4441 gatatatcaa gaattttttt gattaatttt tcatcattac ctattattcc tgctgtatta
     4501 ctacctaatg ctattatgca agctcctgtc aatgtggcca tatcaattat tagtttgggc
     4561 ttataatgtt taattccgaa ggcgagtgcg tcggcaagta ttaatctacc ctctgcatct
     4621 gtgttaatta cttcgacact ttttccatta tacatatgga taatgtcact tggtctataa
     4681 gaagaatttg aaggcatatt ttcaactgca ggaattattc caatcacgtt atgttttatt
     4741 tttaatgaag ataatgattt cataattgca ataatgttaa caccaccaca tttatcaaac
     4801 ttcatttcct ccatcttttc acttggttta atcgatattc caccagaatc aaatgttact
     4861 gccttgccta caagtaaaat tggattctct tcatttttag taccttcata tttcaatact
     4921 attagtttag gagtattgat actaccacct cctacagaaa ctattccgtt taatcccatc
     4981 tctttcatag aatcaagact gataatcttt gatttaatac cttgattttt tatatccgac
     5041 gctattttcg caagatattc tggagaacat tcattaggag gtaaattact gatttctctg
     5101 ccaaaattca cagattgaca tatcacagaa atttcattga ttattttttg gtattctttt
     5161 gaatttgatt ctatcaatat cgaaacattg tcgatagtat tattgttcat cgaagtttta
     5221 aatttttcaa atgaatagga ggaaagtata attccctcta ctaacgattc aattatttcc
     5281 ttagagaagt taaatgggat aatagaaagt tcttttattt tgaattcttt tgcatattga
     5341 attatttttc cagaaataat tcttgatttt tccgtattaa attcattttt gttacctaat
     5401 cctataaata atatttttct agcggggagt tttgcaaatg tgtgtaatac atttaataga
     5461 ccaaatttat gaatattgga aataatttct tttcctgcaa tacttatttc ctcattgaat
     5521 ttattaagta aattggaata atcctcattg agaaaacatc caataaccaa aagttctgtt
     5581 ctcttttcgc ttatctttgt ttcatctgct ataattttta acatatatta tgagatttaa
     5641 caataatatt ttaaattatt gaattgaatt tttaattgat gtgaataatt aattattcct
     5701 ccagctagat ttttatcttt ctttatattt tgtttctcta tgccacattt gtggtttgaa
     5761 ggtattgata tatgatattt cacatgcgtt atctagatct aaaaagaaaa atacaagaaa
     5821 ttagaattaa tgcaatcgaa ggaattgctg aagcaggatc gggacaccca ggcgcttctt
     5881 tttctgctgc tgaaataatg ggtgtattat attttagaga attacgtcat gatccttcaa
     5941 atccatcctg ggatggaaga gattatttta ttaattcaaa aggacattct gcaccaggtt
     6001 attatgcaac gttagcagta gcagggtact ttccaaaaca cgaaatgaaa gatttaagga
     6061 aatttggatc tagattgcaa ggtcatcctg taaggtattc aaaactttta gaacatcatt
     6121 ctgtacctgg agtagaatat tcaggaggct cagaaggtat aggtctttct gtttcaattg
     6181 gaatagcttt ggcaaatgtt attgataaca aagataacaa tgtctatgtt ttactaggtg
     6241 atggtgaaac aaatgaaggt caagtatggg aggctgctat gactgctcct aagtttaaat
     6301 tagataatat tgtagcaatt ttagatcgaa acaaaattca acaagacgga tttactgaag
     6361 atataatgcc tctggaccca gttcgtgata aatgggttgc ttttaactgg aatgcaatag
     6421 aaattaatgg acacaaagtt gaacaaataa tggatgcatt gaacaaagct agtgaaataa
     6481 aggataagcc tactattata attgcaaata ccgtaaaagg aaatgggatt aaacatatgg
     6541 ctaataatcc gcaatggcat ggtaaagcac ctcataatat gcacattcca ttattaattg
     6601 aagaattaga aggagaatac atgatagcac cgtcaataat cgcaggagaa aaagaaaatt
     6661 atgaagaaaa aataaaaaaa gttgaacgag gtggagcaga cattatacat ttagatgtga
     6721 tggatggaat atttgttcct aatattactt ttttagccga tacaattaaa aaattaaggc
     6781 ctataaccaa tatacctttt gatgcacact taatgattga aaaaccgtac gagcatattg
     6841 aggaatatat caacgctggt tgtgatatta ttacaattca tgctgaaacc tgtgataatg
     6901 agaaattctg ctatattcga gagaaggtaa tgtctgcagg aataagtttg ggtatagcta
     6961 ttaatccaca aactgaactt ccagaatggg cattttctta tttgaatgat attgatgtca
     7021 taatagttat gtctgtgaac cctggctttt ctggacaaaa atttattcct gatataacat
     7081 ctaagattag caatttgaat ataactttga aaatgaaggg atacaaagga tatattgaag
     7141 cagatggagg catagatgct tctactgttc aacgagtata tgaagctgga gcacgcatta
     7201 tggtagcagg tagtgcagtt ttttctaatt cagatattaa taccgcaatt ctacagttaa
     7261 aacataaaac aaatgtcacg ttagaaagaa acctattaaa aaaagcagat gaaaatagta
     7321 taagaaaaga ttggattatt gcacgtaagc atattttgat acctgttgct aatgagctgg
     7381 gaatagaaga tgaaattaac agactttgaa ttaagaaaag gggatatgcg atctgcgtat
     7441 ggcaatgctc tggttgaatt aggtacaatt aataaagata ttgtatgtgt gggcgcagat
     7501 accactgatt cattaaaaac aaaaaaattc ggagatcaat ttcctgaacg tttttttaat
     7561 ataggaatag ctgaggcaaa tcttgtttct attgcagcag gtttcgcaat tgctggaaaa
     7621 acagcatttg caagcacata tgctgcattt ttacctggac gttgtttgga tcagattcgt
     7681 aa
//
LOCUS       DASVEV010000221         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_876124, whole genome shotgun sequence.
ACCESSION   DASVEV010000221 DASVEV010000000
VERSION     DASVEV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876124"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF222_09870"
     CDS             <1..451
                     /locus_tag="VF222_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HEX7142771.1"
                     /translation="KRKIPNKNKYVSIEPEIIHQSQILESTKLTLEQLVDVGILIGTD
                     FNPGGIPGIGPKTALKLIRENGRLEEIEKIKDNLKDVPYQQIREIFLNPENISIDSIK
                     FDNPNYKEIIGFLCDTMDFSKERVESSLERIKKSQEKRSQSLERWFK"
     gene            complement(550..2193)
                     /locus_tag="VF222_09875"
     CDS             complement(550..2193)
                     /locus_tag="VF222_09875"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7142772.1"
                     /translation="MTQSFTEEPFSEKEREILLSHFTNSDKPIFAIITPKQVDRGALM
                     SRYSRSDKSMRRIFLEEFANNTNRGDEFYKRVLLEYGDDSVAELGEAQVAIEYVSNIA
                     AKNIEDQRIGLSYLEKSSRYVSFDKKNNGQYKYLRERKIMNSRYADVYLEACDYAFEV
                     YSKNIEVMQKFIIEREPIENFQFYDSISKKDVQFNNLKESNDIKSAQKIYKSTIKAKS
                     LDLLRGLLPASTLTNLGITGNGRAFEYLLTRLYASNLTEMNVLADQLYEELNLIIPSF
                     IYRAKNQHGISMQNYLKNTKDVILRMAKEHLPHIKINANLEYVNLVFNEDNTEAEVKI
                     ASSILFEQAAGHTLTKIIEYVRSLPIEERHKIIKTYTSLRTNRRQRPGRAYEMTEYTF
                     EMLTNFGMFRDLHRHRILTLERQLLSTRHGYDVPKEISDLGILDDFKDCMYKCNEVFQ
                     ILVNDNYLEEAQYVVNFAYRYPFFMKLNLREATHMIELRTLSQGHPDYRYVCQEIFKK
                     IRNVHPILSEGIKFVDLKDYSLERLSSEKNIEKKKREIR"
     gene            complement(2289..2582)
                     /locus_tag="VF222_09880"
     CDS             complement(2289..2582)
                     /locus_tag="VF222_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7142773.1"
                     /translation="MPVAILPDISEQHCIGCALCVEICTALGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKAIFWMRPMDYTPGQPIPLKKNAIFNKGWEEG"
     gene            complement(2695..>3015)
                     /locus_tag="VF222_09885"
     CDS             complement(2695..>3015)
                     /locus_tag="VF222_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7142774.1"
                     /translation="AERQRWLMFRNEEFPLNSAMILVYPKSDLYALWLLNIQYNLDIL
                     WFNEEGKLVYYIEDAKPCENPLDPQNCTFKTTVPAKYIVVSTEGFIKRNNITNTSILT
                     IISI"
BASE COUNT         1056 a          468 c          399 g         1092 t
ORIGIN      
        1 aaaacgaaaa attcctaaca aaaacaaata tgtaagcata gaaccagaaa ttattcatca
       61 atcccagatt ttggaatcta caaaattgac gctggaacaa ttagtagatg taggtatcct
      121 aattggaaca gattttaatc caggtggtat tccaggtata ggccctaaaa ctgcacttaa
      181 gctaataaga gaaaatggca gattggaaga aatagaaaaa ataaaagata atttaaaaga
      241 cgttccatat caacagataa gagaaatatt tcttaatcca gaaaatattt ctatagacag
      301 tataaaattt gataatccaa attataagga aattataggt tttctatgtg atacaatgga
      361 tttttcaaag gaacgagtag aatcatcatt agaaagaata aaaaaaagtc aggagaaaag
      421 aagtcaatcc ttagaacggt ggtttaaata agcataagaa taataaaaat taaaaaaata
      481 atacacataa tcaattgata gtcataaatt tctaaaataa aataaatgga taaaatttta
      541 tctgattgac tatctaattt ctctcttttt cttttctata tttttttcac ttgatagcct
      601 ttctagacta taatctttaa gatcaacaaa ttttattcct tcagataaga tagggtgaac
      661 attccttatt tttttaaaaa tttcttgaca tacataacga taatcaggat gaccttgtga
      721 taaagttctc aattcaatca tatgcgtagc ttctcgtaaa tttaatttca taaaaaatgg
      781 atatctataa gcaaaattga ctacatattg tgcttcttct aaataattgt cattgacaag
      841 aatttgaaat acctcattac atttatacat gcaatctttg aaatcatcca aaattccaag
      901 atcggaaatt tccttaggca catcatatcc atgtctagta gaaagtaatt gtctttccaa
      961 agttaatatt ctatgtcgat gtagatcacg gaacattcca aaattagtaa gcatttcaaa
     1021 tgtatattca gtcatttcat aagctctacc tggacgttgt cttctattag ttcgtaaact
     1081 tgtatatgtc ttaattattt tgtgtctctc ctctattggt aaagatcgaa catattcgat
     1141 aatttttgta agtgtatgtc ctgctgcttg ttcaaacaaa atagatgatg caatttttac
     1201 ttctgcttca gtattatctt cattaaatac taaattcaca tactctagat ttgcattaat
     1261 tttgatgtgt ggaaggtgtt ctttagccat tctgagaata acatcctttg tgttttttag
     1321 gtaattttgc atagatattc catgctgatt ttttgcccta taaataaaag atggaataat
     1381 tagattaagc tcttcataca attgatcagc taagacattc atttctgtta aatttgatgc
     1441 atacaatctt gttaaaagat attcaaaagc tctcccatta ccagtaatac ctaaattggt
     1501 aagcgtagac gcaggtaaaa gcccccgtaa aagatctaaa gatttagctt ttatagtact
     1561 cttatatatt ttttgagctg attttatatc attggattct tttaaattat taaattgtac
     1621 atctttttta gaaatagaat cataaaattg aaagttttct ataggttctc tttctataat
     1681 gaatttttgc ataacttcta tgttcttaga ataaacttca aaagcatagt cacatgcttc
     1741 taaatataca tctgcatatc tagaattcat tatttttctt tctctcaaat atttatactg
     1801 accattattt ttcttatcaa atgaaacgta tcttgatgat ttttctagat aagataaacc
     1861 tattctttga tcttctatat ttttagctgc aatattggat acatattcta ttgctacctg
     1921 cgcttctcct aattctgcaa cagaatcatc accatattct aataatactc ttttataaaa
     1981 ttcatctcca cgattagtat tgtttgcaaa ttcttctaaa aatattctac gcatactttt
     2041 atcggatcgt gaatatcttg acattaatgc acctcgatca acttgtttgg gagttattat
     2101 tgcaaaaatt ggtttatcac tattagtaaa atgggataaa agtatttctc tttctttttc
     2161 agaaaatggt tcttcagtga aggactgcgt catcaataag aaataacgtt attgtatatt
     2221 tatagaatta tttaaaataa ctaatattta gagtactaaa aaaataaaat aaaagagttt
     2281 ggttaattct aaccttcttc ccaacccttg ttaaatatag cgtttttctt taatggaatt
     2341 ggttgtcctg gtgtataatc cattggtctc atccaaaaaa ttgcctttgt tggacagaca
     2401 ccaatacatg caccatcaga gatacatctt tctggataaa atacaaaggc tttacctctt
     2461 ttccaccctt caactggctt tactctgagg acatctggac caagagcagt acaaatttct
     2521 acacaaagag cacacccaat acaatgttgt tcactaatat ctggaagtat tgcaactggc
     2581 atttaattca ctaatttccg attctttctg aaactattta aaccctatgt caaatttata
     2641 acaccgatat attgaatcta ataaatagat atattgaatc tactaaatat aaagttagat
     2701 tgagattata gttaaaattg atgtatttgt aatattattt cttttgataa atccttctgt
     2761 tgatactaca atatattttg caggtacagt tgttttaaag gtacaattct gaggatctaa
     2821 tggattctca cagggtttag catcctctat ataataaact agttttcctt cttcattgaa
     2881 ccacaaaata tcaaggttat attgaatatt caatagccat aatgcataga gatcagattt
     2941 tggataaact aaaatcattg cagagtttaa cggaaattcc tcatttctga acattagcca
     3001 tctttgtctt tcagc
//
LOCUS       DASVEV010000222        15799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1265403, whole genome shotgun sequence.
ACCESSION   DASVEV010000222 DASVEV010000000
VERSION     DASVEV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265403"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2635)
                     /locus_tag="VF222_09890"
     rRNA            complement(<1..2635)
                     /locus_tag="VF222_09890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            complement(2884..4352)
                     /locus_tag="VF222_09895"
     rRNA            complement(2884..4352)
                     /locus_tag="VF222_09895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            4720..6054
                     /locus_tag="VF222_09900"
     CDS             4720..6054
                     /locus_tag="VF222_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7142775.1"
                     /translation="MNIDNLKRNYKKNTKHSNLLYKKSLRFFPGGISHNIRFFTPYPF
                     FVKKATGKYLLDVDGIKYTDYWMGHWALILGHSPKNVSLALSKQVKNGTLYGTVNTTS
                     IELAETIQKLMPKAEKMRFATTGSEATMYAVRLARAVTRKKIVAKIIGGWHGFNSSLL
                     ESVNYPFEINEGLGLDVDNKYIQSIPFNDLHTSLQLLNKIKNDLACIIVEPILGGAGC
                     ITPNKDYLQGLQEFSKKNNIIFILDEIVTGFRLSINGAYKMFKLDPDLFTLGKIAGGG
                     LPIGIVCGKNEILSISNPLDKNNKNSICSIGGGTFSSNPVTMTAGLKTLEFLQKNKKT
                     VYSKINELGAYTRKELQIIFDENNIEAKITGIGSLFNVHFLNRDVREINNANDVALAD
                     KEKLRKYHFALISNYKIFFLPFKMGAISYQHSQVDIDNLLKSTRSIIESKIL"
     gene            6091..7485
                     /locus_tag="VF222_09905"
     CDS             6091..7485
                     /locus_tag="VF222_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142776.1"
                     /translation="MISFTLDLKLIKILLFCFLIGCLIRIIPELIAYPYPIGYDTINF
                     YIPLAGGFQNDPFQFYTYLLYIFKSVIPLSPPSLIIYLSSITYGIFSVSIYLLLKGLN
                     LKPAWFMTIFILFQISALRTTWDLQKDILALSFTFLIFYLILNDRKTYYSKIRIIHFG
                     LIISLVIITLLTDNMISFLLITSLSIYFLIKKDLKYIISLPIIIISIIIFLVLVDNTK
                     NDALINNINNVLTVNIKENVNYSPLNLGILFIMMNILTFPLFIYGLKNLRQLLLCIPL
                     GIALFGSFTWLVLPHSAILLPDRWIIISGIFMSFFSSYGLIKLFSNYEDKPNYKILLP
                     ILSFYIIIGLFYMILPYDYSFPIYWIFAEYTHRFVPSTMQFNSIDIVDNRDLSIVIDW
                     LNNYTTPKSIIHGDPYLRGWMKTSLKDHRIFQYNHNTSSSNGIHIILADKIDSIKDPI
                     KLLYSQGVFRIIEK"
     gene            7536..7979
                     /locus_tag="VF222_09910"
     CDS             7536..7979
                     /locus_tag="VF222_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142777.1"
                     /translation="MKKNNNWFRRRWLDFRQGHSIYLIFLMTFANFIVIQYRLLIDRM
                     PSIDSLIGGNIVGFAATFIAIYVPLSIIIGYWHRKSQWRVEAEALFKENRIGATMWLF
                     VIDLIDGKITEQEKKEMREMLLKITKGNKIPKVEKSDKDNKDNEK"
     gene            8088..9005
                     /locus_tag="VF222_09915"
     CDS             8088..9005
                     /locus_tag="VF222_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142778.1"
                     /translation="MKILHIHDQAGVACILAKYQRMNDIKSKVLSSNKIDKYGILKFY
                     KDYVDLVDRVNFVESCISEAKSADIIHIHSVEQLVIKIRKAFGNSKKIILHYHGTDIR
                     GLKNNNNQSFFNIQNTKTRAKSFGAKVKNRLRLIQMGYYQPLQTESQKLANEILVSTP
                     DLLLLLPQAKYLPNPVDVDHFSKDITNHNKGNNNALTIKTTSGDIQKTLLYCTENNIN
                     LKIDVLDRTKTPILYEEIPNFLKKYDVYVDIRIVNDKILENFSKTALESLACGLKVLN
                     YKLEYVYGLPELHNPVNVTNELKNIYNKI"
     gene            9982..10890
                     /locus_tag="VF222_09920"
     CDS             9982..10890
                     /locus_tag="VF222_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142779.1"
                     /translation="MKILHINDQAGVACILAKFQEKQGVKSKVIAIADKYGIKDYYKN
                     NVILVEKENFYTACLNEAASYDIVHLHSVDALVYKLRRKYGKKKKIILHYHGTDLRGN
                     YKKSITLELRLKRFGVRIKNKLWLLRNGYTGSLQGTMQHQADIVLVSTPDLLKFANNS
                     CYLPNPVDTELFNESTSILNKDYKYDAITIKTEAIDIQKALNYIKDRNISHNIFILDR
                     AHSPTQYKDMPDLLKKYNTYIDIKFVNGKLLESSSKTALESLACGLEVLNYQLKYTKK
                     LPDKHEPLNVVKNLFNIYLSSKDSVK"
     gene            11198..12310
                     /locus_tag="VF222_09925"
     CDS             11198..12310
                     /locus_tag="VF222_09925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7142780.1"
                     /translation="MKILYLTSFGLPNGRIEKSVLTATNNGYEVIFAGRESPQYKNTI
                     FSRMYSITWNAKARFGIPYYWHMVRKQILKIIEDVRPDLIHAHTIFPAKMINDLGIPF
                     IYDDNEHWAQYAKVIYEITNSKGSSRIGRWLTKQMKKRAISVWPTWEKEIVSNHPTIT
                     VSERIATDLKNMGNKKDVFVVPNFPLESEIKNINIPNYNKVINSVYQGRDGISIDIYP
                     HKNLEGLENIFLENDIGNLIFIGWNEKNKEKIFFKGLLPRDLMFAEMSKYDIGLLPWK
                     KHWSHPYLNPNKYAEYAHSGLLIINTSSLETISDTLKGKCETIENYNDLITKIAYYKE
                     NTNVLYKKRIETYNFAKKNLLWDKYEKNILHAYQIC"
     gene            complement(12781..13608)
                     /locus_tag="VF222_09930"
     CDS             complement(12781..13608)
                     /locus_tag="VF222_09930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7142781.1"
                     /translation="MKNVKGRVATIIPARNEEKFIGETLAALLNQDITDNNIIVVNDG
                     SEDKTKEVVNSFPNIELINIENRGFDAHGTPNLAKVINQGLEKLLPDVLYDYIMILGS
                     DHILPSNYIRKIVDQMDENRDIAICSGQIKGEKSKVPRGSGRIVRSDFWKTIGLQYPL
                     KFGFETYLLIKAQQLGYRINVLNDLISLTSRPTRKSYKKETYVSYGKSLRALGYIQLY
                     SAGRIGLISLKNPKGAFYMLKGYTSSDIEFYEEDFRSFLRCIQYVRLKQYIKSFLFR"
     gene            complement(13760..14794)
                     /locus_tag="VF222_09935"
     CDS             complement(13760..14794)
                     /locus_tag="VF222_09935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7142782.1"
                     /translation="MKIAIAGAGVAGSYLGFMLQKEGHDVTIFESSKQENHWAVCAWG
                     ASRNILEKFSKDVGLNFDEYIFHIGKKLRMELPNNKEEYLELIGLVTYNKQKWEHDLL
                     KDLTVNYEFKCSSSFPFRDYDYVLDCTGLHRSLLPKSTTDFIIPAYEYLIENPDMIPT
                     DNEFYVIGYKGARGYFWYFPIDKGRAFVGAGDIDRKYYGIKEFFQNHPSAKIIKKIGR
                     PIRLAPPKRMEPFFNNNVIGVGESIGCVFPMLGEGIIPSLLCCEIFLNVFDKSKNFDF
                     KLYRNLVLKKFNYYEDVYKIVRLKMEGKLSTIKHFPLMMKMYTSMKKDEKRFGFEINL
                     DKMNRLVNTL"
     gene            15126..>15799
                     /locus_tag="VF222_09940"
     CDS             15126..>15799
                     /locus_tag="VF222_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX7142783.1"
                     /translation="MAYMLAAIISFSAVAAITMQPISLSAQTQPAQQQPTATPQSASQ
                     PLAISPSCGQVITQNVILTSNLNCAESDGLIVGASDIVVDLNGHTISGPDVDSDTKTS
                     SKVGIMIPNMNNVVVQDGTIEGFQAGILMTGSQNVEVKGIVAKNNQIGLFSTGASILN
                     AHLSIIMNNQIGIAGHSTQQSTIEDNILFQNTLAGVTLVNSDNSVITLNSITESGNGL
                     YIDNQS"
BASE COUNT         5203 a         2883 c         2495 g         5218 t
ORIGIN      
        1 aatgggcgag cagccccacc cttggctcct gctgcagaac caggatggga agagccgaca
       61 tcgaggtacc aaaccgcggg gtcgatagga gctctcgccc gcgacgagcc tgttatccct
      121 agggtaactt ttctgtcacc atcagcctcc aatagtgagg acatgatgga tcgctaagcc
      181 cggctttcgc ctctgaatcc cgtatcattg gggatccagt cagactggct tgtggctttg
      241 ccctcaccgg cggagttctg tcccgcctga gccaatcttt gggccccctc gatatttttt
      301 caagggggtg ccgccccagc caaactgccc acctgccggt gtccccgtga gaacgggtta
      361 gcaatacgat aaccaatggc tggtgttaca ctttcgcctc gaccaatccc agagaattgg
      421 cttcgaaggc tcccaactac tctctgcatt ggacatcata tcgcaacaac aggctgcagt
      481 aaagctccac agggtcttct ctccccgaca gaggtttgtg gactgttcgt ccacgttatg
      541 tggcttcacc gggttgtagg cggggacagc ggggccctcg ttgttccatt catgcacgtc
      601 ggaacttacc cgacaaggca tttggctacc ttaagagagt cagagttact cccggcgtta
      661 aacggccctt agcccagttg aacccaggtt ttaggtaccg tcactggcca ggatacatag
      721 gccgtacaca tcctttcgga cttgcgacca actgtgtttt tattaaacag tcgggacccc
      781 ctagtcactg cgacctgcaa ttcctattcc tcataaaaac tgcaggcact ccttatacca
      841 tagatacgga gctattttgc cgagttccct cgccttacgg tatacccgac agaccttaga
      901 ttactaatcc agggcaccag tgtcggttct cggtacggac ataaaagaat ctcccttaac
      961 ttgctttcag tgtctcctgg attcaagtaa accctccttg cggaaggcca ttctcatctc
     1021 atcaaggttc tcactattac agtactcccc ctgatttgaa taattagaca gaacgacgat
     1081 tctgcttacc ctatcctgaa gcgacaagcc aggtacaaac gatctttcat ggcactggaa
     1141 tattaaccag tttccctttc ggacaacgct gttgaggttg ttcttaggac cggctaactc
     1201 ccggctgatg acgcattgcc gggaaaccct tgcccttgcg gtcaccgaga ttctcactcg
     1261 gttatgctgt tactaccacc aggatctgca ctagtacgat cagtccactg gacttcacag
     1321 cccagcttct acccaatcac tacgcccacc taccatatac cagccttgct ggtaatctaa
     1381 agtatcggta atctacttga gccccgtgca ttttcggggc ttccaagctc ggcaggtgag
     1441 ctgttacgca ctctttaaag gatggctgct tctgagccta ccttcctgct gtctgtgctc
     1501 gaaaacaccc tttggtttga cacttagtag atatttgggg accttaactt tagtctgggt
     1561 tgtccccctc tcggttatga ggcttacccc acataaaccc gcttcctagc ttctacgacg
     1621 cttgtacctt cggagttcga atggacagcg agccctttcg agcccttact gtccgatcgg
     1681 tgctctacag tacaagcaat ctcgactaag acaggactgc gatccaattc ggtgggaact
     1741 agcgatcacc ggactagatt ggtttttgac ccctaatccc agattagagg accgatttgc
     1801 acgtcaggac ccctgcagac ctccagcagg ctttcgccca ccttcgtctt atccaggaat
     1861 agatcgtccg gtttctagcc ttattgctat gactaaagac cctttcagat ctctcgcctt
     1921 gctaatgctg cgcgaatttg gtttcccttc gccttccctt ttaaaggtta agctcgccat
     1981 aacagtaatc tccctggccc gtgtttctag acggaacgta taactctaga aggcttcaaa
     2041 tccttacgga ttctctacct gattcctttc gagccataca cgtctgtaac cggttggttt
     2101 caggcgcttt taacccccct tccggggtac ttttcaactt tccctcacgg tactagtacg
     2161 ctatcggtct tagataatat ttagccttgg atgctacttt cacccacatt cactggccac
     2221 tgtcaaggcc aactactctt gttatagaac aatcctattt ttatttgtct acgggggtat
     2281 cacccttttt cccagttctt tccagaacac ttcgactaaa aaaacaggat ctaaaaacta
     2341 taactcacca catctctata atgttaccat taaagattca gtttgggctg ttccgttttc
     2401 gctcgccgct acttacggaa tctcgattga tttctcttcc ttgtcctact cagatgcttc
     2461 acttcggaca gttcgccctc cccagcataa agcaggggag tattaagaga gacctcattc
     2521 ggaaatcctg agatcgatgg tcgcatgcac ctaccccagg cttatcgcag cttgccacgt
     2581 ccttcatcac tttctaagcc tagtcatcca ccaaccggcg tagttgcacc agcatttccc
     2641 tttgaaattt ggttagtaat tttacgcggt cagatatacc actttgataa tgaagccaat
     2701 taacctctgt cattattgca tattatgcaa tattgtggtt aattgtcgat cctatacacg
     2761 atgatcattg taaactcacg tctactacat cacttcattc ctaaaaattt tctaggaatt
     2821 gcattctttt tgattggttg atttggttac aaacccttta cacggattgt ccgaccttta
     2881 ctaaggaggt gatccggccg caggttcccc tacggccacc ttgttacgac ttctccctcg
     2941 ttgctgaccc caggttcgat agtgccaatg agacacaacc tcgccaagaa ccaacttcga
     3001 tgaagcgacg ggcggtgtgt gcaaggagca gggacgtatt caccgcgcga taatgacacg
     3061 cggttactag ggattccata ttcatgaggg cgagtttcag ccctcaatca taactgaggt
     3121 aaggtttgga gattacctcc tcctttcgga ataggaactt attgtcctta ccattgcagc
     3181 ccgcgtgtgg cccgagggtt tcggggcata ctgacctgcc gtagccccct ccttcctccg
     3241 tctcagcgac ggcagtccct ctaattagcc ccattactcc tgagagcaat ggtagcaact
     3301 agaggcaggg atctcgctcg ttacctgact taacaggaca cctcacggca cgagctggcg
     3361 acggccatgc acctcctctc agcttgtctg gtaaagtctt cagcttgacc ttcattctgc
     3421 tgtcgccccc ggtaagattt ccggcgttga ctccaattga accgcaggct tcaccccttg
     3481 tggtgctccc ccgccaattc ctttaagttt cagtcttgcg accgtactcc ccaggcggca
     3541 gacttaacgg cttccctgcg gcactgaaga ggcacgaagc ctattcatca ccgagtctgc
     3601 atcgtttaca gctgggacta cccgggtatc taatccggtt tgctccccca gctttcatcc
     3661 ctcaccgtcg agtgcgttct ggcaagccgc cttcgccact ggtggtcttc aatggatcaa
     3721 cggattttac ccctacccac taagtaccgc ttgcctctcc cgcctcctag ctccatagta
     3781 tcctccgcag cccatctgtt aagcaggtgg atttaacgga agacttgtag aaccggctac
     3841 ggatgcttta ggcccaataa tcgtcccgac cactcggggt gctggtatta ccgcggcggc
     3901 tgacaccagg cttgcccacc ccttattctg caccattttt agaggtgcca aaagatttcc
     3961 ttagcggaaa tcactcagaa taaccctgtc aagctttcgc ttattgcaga gttttcgcgc
     4021 ctgctgcgcc ccatagggcc tgggcccttg tctcagtgcc catctccggg cttctcctct
     4081 cagagcccgt accggtttta ggtttggtgg gccattacct caccaacagc ctgatcggcc
     4141 gcagtccaat ccaggggcca tatagattta agccatacat cattccagat attatgacct
     4201 atcgcggttt atcctcagtt tcccgaggtt atccacgtcc cctgggcatg ttgactacgt
     4261 gttactgagc cgtatgccac gtattgctac gttgactcgc atggcttagt cctattctga
     4321 tagcagtcgg gtccggcagg atcaaccgga gttaatcctt taataatatt aaataaagag
     4381 tacggccgga caatcatgaa ttgtgcgggt ttgtaaccta tatcagatct aataggttat
     4441 attaaatcct cataatatga gcgcaaatgg agatcaataa gtcacatttt ggtaccatga
     4501 ccaacatcat tattgatgcc gccttgcctt gcgttctaga ttccatcttc aactgcatca
     4561 tataaacctt aggtttatat tttttgcaat aaataagatg gactaataat ataatagtct
     4621 aaccaatata tatttaccta aaaattatgt taaaaataaa tacaaatttt tctattcata
     4681 acttataaat tagttctttg attttgtctg ttttcatata tgaatattga taatctcaaa
     4741 agaaattata agaaaaatac aaagcattct aatttacttt ataaaaaatc attacggttt
     4801 tttcctggtg gaattagtca taatattaga tttttcaccc cttacccttt ttttgttaaa
     4861 aaagctactg gaaaatacct tcttgacgtg gatggtatta aatatacgga ttattggatg
     4921 ggccattggg cattgatttt aggtcattct ccaaaaaatg tttccctagc gctatctaaa
     4981 caagtaaaaa atggtacatt gtatggtact gtaaacacta caagtattga attggctgaa
     5041 actatccaaa aactaatgcc aaaagcagaa aaaatgagat ttgctacaac aggctctgaa
     5101 gctacgatgt atgcagtaag acttgctcgt gcggtaacca ggaagaaaat agttgctaaa
     5161 ataattggag gatggcatgg atttaactct tccttgcttg aaagtgtaaa ttatcctttt
     5221 gaaattaacg aagggttagg attagatgta gataacaaat acattcaatc aattcctttt
     5281 aatgatctac atacatcttt acaattatta aacaagataa agaacgattt agcatgtata
     5341 attgttgaac ctattctagg cggtgctgga tgtattactc ctaataaaga ttacctccaa
     5401 ggattacagg aattttcaaa gaaaaataat attatattca tactagatga aattgtaact
     5461 ggttttagac tctcaattaa tggagcttat aagatgttta aattagatcc agatttattt
     5521 actttaggta aaattgcagg tggaggatta ccaataggca tagtttgcgg taaaaatgaa
     5581 attttatcca tttctaatcc acttgataaa aataataaaa attcaatttg ttccattgga
     5641 gggggtacgt tttcctctaa cccagttaca atgactgctg gattaaaaac tcttgaattt
     5701 ttacaaaaaa ataaaaaaac agtttactca aaaattaatg agcttggtgc atatacaaga
     5761 aaagaacttc aaataatatt cgatgaaaat aatattgagg caaaaataac tgggattggt
     5821 tcacttttta atgtacattt tttaaataga gatgttaggg aaattaataa tgcaaatgat
     5881 gtagcgttgg ctgataaaga aaaactaagg aaatatcatt ttgctttaat ttctaattac
     5941 aaaatattct ttttaccatt caaaatggga gcgatttctt atcaacattc tcaagttgac
     6001 atagataatt tattaaagtc aacgagatct ataattgagt ccaaaattct atgatagtta
     6061 tatatacatt ataaaattac ttaattaaag gtgatttcat ttactttaga tctcaaatta
     6121 attaaaattc tcttattttg ctttcttata ggatgtttaa taagaattat tcctgaactt
     6181 attgcttatc cttatccaat tggttatgac actataaact tttatattcc tttagctggt
     6241 ggttttcaaa atgatccatt tcaattttat acatatttac tatacatatt taaatcagtt
     6301 attcctctat ctccgccgag ccttataata tatttatcat caataacata tggtatattt
     6361 tcagtttcaa tttatttatt attaaaaggg ctaaatttaa aaccagcttg gtttatgact
     6421 atatttattt tatttcaaat ttctgctttg agaactactt gggatttaca aaaggatatc
     6481 cttgcattgt catttacttt cttaattttt tacttaattt taaacgacag aaaaacctat
     6541 tactccaaaa taagaataat tcattttggt ttgatcattt cattagtaat tatcactctt
     6601 ttaactgaca acatgatttc ttttttatta attacatcat tgtcgattta ttttttgata
     6661 aaaaaggatt taaaatatat tatttcttta ccaataataa ttattagtat aatcattttt
     6721 ttagtattag ttgataatac taaaaatgat gcacttatta ataacataaa caatgtatta
     6781 actgtcaata taaaagaaaa tgtaaattat agtccactaa atttaggaat tctatttata
     6841 atgatgaata ttttaacatt tccacttttt atttatggtt taaaaaattt gcgtcaacta
     6901 ttgttatgca tcccattagg tatagcttta tttggatctt ttacctggtt agtattacca
     6961 cattcagcaa tattattacc agatcgatgg attataattt ctggaatatt tatgtcattt
     7021 ttttcttcgt atggattgat caaattattc tccaattatg aagataaacc aaattataag
     7081 atacttttac ctattctttc tttttatata attattggct tattttacat gatattacct
     7141 tatgactatt cttttccaat ttattggata tttgcagaat atactcatcg ttttgttcct
     7201 tccacaatgc aatttaattc cattgatatc gtagataata gagatctctc aatagttata
     7261 gactggttaa ataattatac tactccaaaa tccattattc atggagatcc atatctcagg
     7321 ggatggatga aaacgtcatt aaaggaccat cgaatatttc agtataatca taatacatcc
     7381 tcaagtaatg gaattcacat catattagca gataaaattg attcaataaa ggatccgata
     7441 aaattgcttt attcacaagg tgtttttagg attatagaaa aataacaaca tctcattagt
     7501 aaacaattta tctaaattaa tagaactacg aaatattgaa aaaaaataat aactggttta
     7561 gaagacgatg gttagatttt agacaaggtc atagtatata tcttattttt ttaatgacat
     7621 ttgcgaattt tatcgttatt caatatagat tattgattga caggatgcct tccatagatt
     7681 cgttgatagg aggaaatatt gttggatttg cggcgacatt catagctata tacgtccctc
     7741 tttcgattat tataggatat tggcatcgaa agagtcaatg gagagtagaa gctgaagcat
     7801 tatttaagga gaatagaatc ggagcgacta tgtggctttt tgtaatagat cttatagatg
     7861 gtaagatcac tgaacaagag aaaaaagaaa tgagagaaat gcttttaaaa attactaaag
     7921 gtaacaaaat tccaaaggtt gaaaaatctg ataaagataa taaagataat gagaaataat
     7981 tatgaatcat aaccatagat aatatttatt ttaaaagaat caaatttcat taactaatta
     8041 ttcttatttt ctaaatattt ttatctagtt tattgtttac ttgtttattg aaaatattgc
     8101 atatccatga tcaagctggt gtggcatgta tcttagccaa atatcagaga atgaatgata
     8161 taaaatcaaa agttttatca tccaataaaa ttgataaata tggaatatta aaattttata
     8221 aagattatgt tgatttagtt gatcgagtca attttgtaga atcctgtatt tctgaagcaa
     8281 aaagtgcaga tattatacat attcatagtg tggagcaatt agtaattaaa atacgcaaag
     8341 cattcggaaa ttcgaaaaaa attattcttc attatcatgg tacagatata cgtggcctga
     8401 aaaataataa taaccagagt ttttttaata tccagaatac taaaactaga gcaaaatcat
     8461 ttggagcgaa agtaaaaaat aggttaagac tcatccaaat gggatattat cagccattac
     8521 aaactgaatc tcagaaatta gctaatgaaa tattggtttc aacaccggat cttttattat
     8581 tattacctca ggcaaaatat cttccaaatc ctgtcgatgt agaccatttt tcgaaagata
     8641 ttactaatca taacaaagga aataataatg ctttaaccat aaaaactaca tctggggata
     8701 ttcaaaaaac cttactttat tgtactgaaa ataatattaa tctaaaaata gatgtacttg
     8761 atagaacgaa aacacctatt ttatatgaag aaatccctaa ctttttaaaa aaatatgatg
     8821 tgtatgtaga tataagaata gtgaatgata aaatacttga aaattttagt aaaactgcat
     8881 tagagtctct cgcttgtgga ctaaaggttt tgaactataa attagaatat gtatacggac
     8941 tacccgaatt acataatcca gtaaatgtta caaatgaact taaaaatatc tataacaaga
     9001 tttgatttca tttctaagag tagtaagtaa aatcacgaat gtattatatc atctattcaa
     9061 aacatttaat agaatatacg ttttcatagt aatgaatata aatatatata gtcatttaat
     9121 caattgtaaa gataagaaat ttacattatc aagagataaa actcttgaat aggtaacaaa
     9181 tatttttatt tgtcgaatgt aattaagaat tattaaacaa caacattttg ttcagtttta
     9241 aacagaaatc taacctttca tacttaaata ttgttaacgt ttgggatgga ttgagtaatt
     9301 tgagatgatc aagagaataa acatgagaaa taaattacta taatcttaga tattctctat
     9361 tatgataaat gaattaagaa agttagatta caacaaagct aatactgttt cactaatttt
     9421 acaggaacct atactgtcta tagatttaaa gttgtttatt taacttaatt cgtattggca
     9481 acatattttt tatggtaata aaccttattt tttcattgca tttatatcct aacagaagtt
     9541 tagtagttta gagtatcgct taatattata ctagttcact tatgcatggt aaattaaatt
     9601 ataagagttc tatttctata ttttgatatc attctaattt atggtatcat aattaaagtg
     9661 gctatagtct attaatttat tattataaga tacatttaac catttatatg ttaagcattt
     9721 gatcgtttac tctttctttg ctagtgatgt tacttgatat acatcatatc ttgaaacaat
     9781 attattatac tttttacctt caggtttcct ctcaatcaat ctcttaagct gagaccaata
     9841 gagtataaat gatctctatg ttaataaagt aaaacaatat tcattgggat aaataggttt
     9901 tacaatttgc taagtcattt cattaaacac tattcaaaaa tattattagt aactctgaaa
     9961 ttacttaaat agaaacaaaa tttgaaaatt ttacacatta atgaccaggc aggtgtagct
    10021 tgtatattag caaaatttca agaaaaacaa ggtgtaaagt cgaaagtaat agctatagca
    10081 gataagtatg gaattaagga ttattataaa aataatgtaa tattagtaga aaaagagaat
    10141 ttttataccg catgtttaaa tgaagctgct tcttacgaca tcgtacatct gcacagcgtt
    10201 gatgcattag tatataaatt acgtagaaag tatggaaaaa aaaagaaaat cattttacat
    10261 tatcacggaa ctgatctacg tggcaactat aaaaaaagta ttactttaga attgagatta
    10321 aagcgattcg gagtgagaat taagaataag ttatggttac ttagaaatgg ctatacagga
    10381 tctctgcaag gtactatgca acaccaagca gatattgtat tagtctctac tccagatcta
    10441 ttaaaatttg ctaataattc ttgttaccta ccaaatcctg tggatacaga attatttaat
    10501 gaaagtacaa gtattttgaa caaggattat aaatatgatg caattactat taaaacggaa
    10561 gcgatagata ttcagaaggc tttaaattat atcaaagaca gaaatataag tcacaatata
    10621 ttcatacttg atagagcaca tagtcctact cagtacaaag atatgccaga cttattaaaa
    10681 aaatacaata catacataga tataaaattt gttaatggaa aacttttaga aagttcaagt
    10741 aagacggctt tagaaagtct agcatgcggg ttagaagttc ttaattatca attaaaatat
    10801 actaaaaaat tacctgataa acacgaacca ctaaatgttg taaaaaattt atttaatatc
    10861 tatttaagta gtaaagattc agtcaagtaa tatgatagaa ttctaattaa aattttatta
    10921 ttttaataga gattcaagta aaaactattc taaaataatc tgtgaagaaa tattttctaa
    10981 ttacactcta tgatagatac tttatttgag atgcagaata ataatatgag accaaaatat
    11041 catataatta gaatacttcg taggtaattc tattatatat aacctacaaa cctataaata
    11101 atcagacctt tagatgaaaa tatcctttca tattttagag aaactaaaga tcttagccga
    11161 aagttttact aaatgcttta catctttatt atagtgaatg aagattttat atctaacaag
    11221 ttttggacta ccaaatggta ggattgaaaa atctgtttta actgctacaa ataacggtta
    11281 tgaagttata tttgcaggaa gagagtcacc acaatataaa aatactattt tttctagaat
    11341 gtactctatt acatggaatg ccaaagcaag atttggaata ccgtattatt ggcatatggt
    11401 tagaaaacaa attttaaaaa ttatagaaga cgttagacct gacttaatac atgcacatac
    11461 tatctttcca gctaagatga ttaatgattt aggaattccg ttcatatatg atgataacga
    11521 acattgggct caatatgcaa aagttattta tgaaataaca aattccaaag gttctagtag
    11581 aataggaaga tggctaacca aacaaatgaa aaagcgagca atatctgtat ggcctacgtg
    11641 ggaaaaagaa attgtatcaa atcatcctac tattactgtt tcagaacgca ttgcaactga
    11701 tttaaaaaat atgggaaaca aaaaagatgt atttgttgtt ccaaattttc ccttagagtc
    11761 tgaaattaaa aatatcaaca tcccaaatta taataaagtg attaacagtg tttatcaggg
    11821 tagagacggc atatcaatag atatctatcc tcataaaaat ttggaaggat tagaaaatat
    11881 atttttggaa aatgatatag gcaatttaat ttttatcgga tggaacgaaa agaataaaga
    11941 aaagatcttt tttaaaggtc tattacctag agacttaatg tttgcagaaa tgtcaaaata
    12001 cgacatagga ttgctaccat ggaaaaaaca ttggtctcat ccttatttga atcccaataa
    12061 atatgcagaa tatgcacatt ctggtctatt aattattaat acatcgtcat tggaaacaat
    12121 ttctgataca ttaaaaggaa aatgcgaaac tattgaaaat tataatgatt taattacaaa
    12181 aattgcgtat tataaagaaa ataccaatgt tttatataaa aaaagaatag agacctataa
    12241 ctttgcaaaa aaaaaccttt tatgggataa atatgagaag aatatcctac atgcctatca
    12301 aatttgttaa taaatcttct tatatgtaac tccatgagat ttacaataat tctttgatgt
    12361 attatcatat aggttaccta attagagaaa ctaattttta aataattcta tactctgtta
    12421 agagttataa ttgtataaag tatttctatg agtagagtga atatgttcat ttatatttaa
    12481 caacaaaacc atctttttaa tacctagtta cgctttagaa tgtttattgt aaggttataa
    12541 aagacattct gtcaatttat gcttgttcat cacgagcata tacatatcct gtgtcgacgt
    12601 ttgaattgtt ttcttcatga cactattttt tgttcctttt aataatttgt agtgtccctt
    12661 ccagttttaa ggttattaga cactcagtca taagaataca taatatcaaa tcacatttta
    12721 gatgtaagtg ataccttaat tattaggttt attattcacc actttttatg tccttataga
    12781 ttacctaaat aaaaatgatt ttatatattg ttttaatctt acatattgaa tacatctcag
    12841 aaaacttcta aagtcttctt catagaattc aatatcacta ctagtatacc ctttaagcat
    12901 gtagaaagca cctttcggat ttttcaaaga aattaagcca attcttcctg cagaatataa
    12961 ttgaatataa ccaagagctc taagtgactt tccataagat acataagttt ctttcttata
    13021 agattttcgc gtaggtctgc ttgtaagact aataagatca tttaatacat ttattctata
    13081 acctaactgc tgggctttta tcaatagata agtttcaaaa ccaaacttta atgggtattg
    13141 aaggcctata gttttccaga aatcacttcg aacgattcta cctgatcctc ttggaacttt
    13201 acttttttca cccttaatct gtccagaaca aatggctata tctctatttt catccatttg
    13261 atctacaatt tttcttatat aatttgatgg gagaatatga tcagatccca aaatcattat
    13321 ataatcatag agaacatctg gtaataattt ctcaagacct tgattaatta cttttgctag
    13381 atttggtgtc ccatgagcat caaatcctct gttttcaata ttaattagct caatattagg
    13441 gaaagaatta acaacttctt tagttttatc ttccgatcca tcatttacta caataatatt
    13501 attatcggta atatcttgat ttaaaagtgc agctaatgtt tctcctataa atttctcttc
    13561 atttctagct ggaattatcg ttgcaactct tccttttaca ttcttcataa acattgaaga
    13621 tttattaaaa ttagattaaa tgttttttgt taaactataa tgattagtag catttgttca
    13681 ttaatacctt tttaaagaaa atattcaaat cttttaacga gatataacta taagttgtgt
    13741 cgagaaaaaa aagatataat taaagagtgt tgactagcct attcatttta tctaaattta
    13801 tttcaaatcc aaatcttttt tcatcttttt tcattgacgt gtacattttc atcattaaag
    13861 gaaaatgttt aattgtactt aatttgccct ccattttcag tcttacaatc ttatagacat
    13921 cttcataata attaaatttt ttcaaaacta aatttcggta caatttaaaa tcaaagtttt
    13981 tagatttatc aaatacattt aaaaagattt cacaacataa caatgatggg attattccct
    14041 cccctaacat tggaaaaacg catccaatag actctcccac tcctataaca ttattattaa
    14101 aaaaaggctc catacgtttt ggtggagcta gacgtatagg tcttcctatt ttttttatga
    14161 tttttgctga tgggtgattt tgaaaaaatt ctttaatccc ataatatttc ctatcaatat
    14221 ctccagcacc tacaaaggct ctacctttat caattggaaa gtaccaaaaa tatcccctcg
    14281 cccctttgta acctatcaca tagaactcat tatcagtagg tatcatatca ggattttcta
    14341 tgagatattc ataggcaggg ataataaaat cagttgttga ttttggcagt agagaacgat
    14401 gaagacctgt acaatcaaga acataatcat agtccctaaa aggaaaacta ctagaacatt
    14461 taaattcgta attaaccgtc aaatctttta agagatcatg ttcccatttt tgtttgttgt
    14521 aagtgactag tccaataagt tctaaatatt cttctttatt gtttggtaat tccattcgta
    14581 atttttttcc aatatgaaaa atatattcat caaaatttag tcctacatct tttgaaaatt
    14641 tttctagtat atttcttgat gcaccccaag cgcaaactgc ccaatgattt tcctgcttgg
    14701 aagattcaaa aatggtaaca tcatgtcctt ctttttgtaa catgaatcct aaataacttc
    14761 cagcaactcc agctccagca atagctattt tcatatcaaa acaatcttaa attttcctta
    14821 taattcttta ttaaaactag tttaataatc tttatagatt ataaatgcag aaaatttatt
    14881 atctaaaata tcaaaaagaa ttgactgtct cttctataaa tgttattgca acaaattaaa
    14941 aaattttaaa tttttgtttg aatccagtca ttaaaatttt aactctgtat aagaattctt
    15001 taaaaagtat caagtgtact attaaatagt tacaataaaa atcgtctata cttagttata
    15061 ataaaaataa tagattaaca taaatatgtc agtaccaaac catctactaa atgaatagaa
    15121 cactaatggc atatatgttg gcagcgataa tctcattctc tgccgttgca gcaattacaa
    15181 tgcaacccat atccttaagc gcccaaaccc aaccagcaca acaacaacca acagcaacac
    15241 cacaatcagc atcacaacca ttagccataa gtccatcttg cggtcaagtt ataacacaaa
    15301 atgttatatt aacttcaaac ttgaattgtg cagaaagtga tggtcttata gtaggtgcga
    15361 gtgatattgt tgtagacctt aacggacata caatttctgg tccagatgta gatagtgata
    15421 ccaagacttc aagtaaagta ggaatcatga ttcctaacat gaacaatgta gtagtccaag
    15481 atggtactat tgaaggattt caagctggta tactaatgac aggatctcaa aatgttgaag
    15541 taaaaggaat agttgctaaa aataaccaaa taggattatt cagtaccggt gctagcatac
    15601 tcaatgcaca tttgtccata ataatgaata accaaattgg tattgcagga cactctacac
    15661 aacaaagtac tattgaagat aacatacttt tccaaaatac cttggcagga gtaacattag
    15721 ttaactcaga taactctgtt atcactttga attctattac tgaatctgga aatggtcttt
    15781 atatagacaa tcaaagtaa
//
LOCUS       DASVEV010000223        14645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_493982, whole genome shotgun sequence.
ACCESSION   DASVEV010000223 DASVEV010000000
VERSION     DASVEV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14645
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493982"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VF222_09945"
     CDS             complement(<1..1061)
                     /locus_tag="VF222_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7142784.1"
                     /translation="MENFFRRKVTDKFNFQEKDKKPSELNNTNNINDIQTFDLYNKGI
                     NHMANEKLTDAIRCFELSLRIDPHFVNAWIKKGYAHFHLGEYGVAISCYDNALDLDIN
                     NFDAWDLKGLAHYRLNNLLKAIECCEKSIDINHNNGMSWYNLSCYLVLDGRIDDGMEA
                     LKRAIEIEISYAKRAVRDKDFENAKAEEGFRRIIEVVVLEAIRHGYDYSGKIIWITGM
                     DQVEVDDALLRLSMKGLIIRKEKKNFFSKEEYFELSREIGSKVGTLKRSGFMGKSREV
                     SPPVQQLKDISEILAKTRESVEQGDVQLTIAYFDKLINPSIHGSTMIEKFFNEHRDLR
                     LYQNRLKDKGQEYLNSHKS"
     gene            1197..1619
                     /locus_tag="VF222_09950"
     CDS             1197..1619
                     /locus_tag="VF222_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7142785.1"
                     /translation="MSDYYKILGITKDSNQIQIRKAFRELALQYHPDKNKNSEESKER
                     FMEIVQAYEVLSDVKARERYDFNTVNEKKFQKFNWTPSADFANFYSYESLKREYNDNQ
                     ISGGMWDISEKANAGLWKATFILLGSLGLISVFIILKI"
     gene            1718..2014
                     /locus_tag="VF222_09955"
     CDS             1718..2014
                     /locus_tag="VF222_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX7142786.1"
                     /translation="MKVLAIGNRIFVTSFQMAGIEGIDVQTPKEALARINQIHSRNEA
                     GLILLSDDIGKEIRLDLARIRSVKPIPLIFELPSPGSKKEKVDYRALLKQILGV"
     gene            2080..3420
                     /gene="thiD"
                     /locus_tag="VF222_09960"
     CDS             2080..3420
                     /gene="thiD"
                     /locus_tag="VF222_09960"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7142787.1"
                     /translation="MNIRSVLSIAGSDSSSGAGIQADLKTFSSLGTYGCSAITAITVQ
                     NTKDIFDIFPLPHEIVMKQIKFILKDIRIDSIKIGMVYDEILINKIFNILKNINIPII
                     VDPIFTSTSGKNLIKPEAIKVFKKKILSIATITTPNINEAKELTGIEINNKEDSVKSL
                     TKIKKLGPQNVILKGGHIFSERSIDTLIDDKLNIIEFSNPRIHIPENHGSGCNFSAAL
                     SAYISQGYEIHIACKFANEFINRVLKNILHIGKGYPVADSAILTSSSSFKFQVIEELS
                     IAIKELENLSGLGKLIPETQSNFAYAIPNATSIKEVAGIKGRIVRIDEKVKASSCIEF
                     GASRHIASAVISYMTINPAMRTGFNIKYDKKIIELLRTFMKVAEYDRRLEPRTIKEKE
                     GYSIKWGVKQAILKNKAVNGIFHKGDLGIEPMIIIFGHKPVEILEYLRKIIEYY"
     gene            3459..4166
                     /locus_tag="VF222_09965"
     CDS             3459..4166
                     /locus_tag="VF222_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7142788.1"
                     /translation="MQAVILAGGLGTRLQPYTFFIPKPMLPLGNKPLLEYLIEWIKKL
                     RNIDEVIICVSYLHRTIEDYFEDGGRFGIKITYAHSERPLATGGQLKTAEKMLNDRFI
                     CLYGDSVYDFNLEKMVNGHEKSKAFLSMALMKYKTRMKYGFIDTNDKGDIVTAWREKP
                     EVEGLINIGCYIMEKEFLRMIPKSSTIGMDKIVKDVITKKKKVKAFTIDSGFIDIGDK
                     RSYLEAYKKYMKKLGSI"
     gene            4262..4615
                     /locus_tag="VF222_09970"
     CDS             4262..4615
                     /locus_tag="VF222_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HEX7142789.1"
                     /translation="MINTNRDKNLSVDASRNTNLNFYVRKMYYQGSLMINICDEELLG
                     TNIVSESLNINITNDFFNESVNEKDICNLLKRCSIANLIGRRVIEKTLSLRLAKRDSI
                     KIVSDIPFLMIFKFD"
     gene            4703..5263
                     /locus_tag="VF222_09975"
     CDS             4703..5263
                     /locus_tag="VF222_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7142790.1"
                     /translation="MSYQRTLRIQKERIGAIIGKNGLVKDEIEKKCKIDIHIDSEYGD
                     VILKSKNTDPLDILNINKGLEVILAISKGFSPERAFILFTEENSLSVIDLRDYVGKSA
                     NSLARVKSRLIGEKGKSRRTIEELAGSYISIYGHYVGIIGTSEQITLTTEAITRLCSG
                     RSHKNVYNFLQEIRRKAKIDKLKLWE"
     gene            5322..5546
                     /locus_tag="VF222_09980"
     CDS             5322..5546
                     /locus_tag="VF222_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142791.1"
                     /translation="MTNPQDERKKVSKISYYKLSTLLAIIITIPGISVLMALRHYDVD
                     IIYQIIVSSVVFFICLGLSFKISNQLVKFG"
     gene            complement(5551..6111)
                     /locus_tag="VF222_09985"
     CDS             complement(5551..6111)
                     /locus_tag="VF222_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX7142792.1"
                     /translation="MGIIIAITGGSGVIYGIKLIESLNSLKLETHLVISEWGEKTIKI
                     ETNLDIAYVQKLSTRWYDNKNMAAPISSGSFKTDGMVIIPCSMKTLASIANGLDDSLI
                     SRAASVCIKENRKLVVVPRETPLSKIHLENMVKLSELGVSILPAMPGFYYHPRTIDDL
                     LFHIVGKVLDQFNIENNMFTRWGVKK"
     gene            6189..7085
                     /locus_tag="VF222_09990"
     CDS             6189..7085
                     /locus_tag="VF222_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7142793.1"
                     /translation="MLTIFGTIALDTTRTSAKTVNRVMGGAATYASISSSKFTSTNII
                     GIIGYDFPQQYKDILNRHVKTKGIIVKDDEKTFHYDSSFDQTLTIRTANKTELNVIEN
                     FEPFVPEELLDSKYIFLANNDPAQNKKMQSLFSDPEFVVCDTIEYWINNKYDQVIDMI
                     QKVNGIIVDSQEARLLTNEYNLIKCAKKIKELGPELIIIKKGENGSMLFYEDIICVYP
                     AIPIENVVDPTGAGDCYAGGFLGYLSKKGKINSKTIREAAIYGNIMGSFAIEDFGLNK
                     IINISSSDIEKRLKLFKELVMV"
     gene            complement(7147..7938)
                     /locus_tag="VF222_09995"
     CDS             complement(7147..7938)
                     /locus_tag="VF222_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HEX7142794.1"
                     /translation="MIEITSSYQSRINNCSNKKKSPIILALDYDLDNYNNSFLLDKCK
                     EVISLSESHICGIKINFHLLLSLSYIQILELNILAHKFDLQSIADIKLNDIGNTNKVT
                     VDKLQKLGFDAVIVNPFIGVTEMKSIVNYAHSIKFGIISLVYMSHPTSFEGYGLKISI
                     SNDEHGDNRESIPMYQILYGYARLSGADGIVIGGSKIDVIKEFSSLNEIKIYSPGFFT
                     QGGSIADAVKAGTDYFIVGRHILHSSSPVNDLKLILYQITKYKNE"
     gene            complement(7931..8617)
                     /locus_tag="VF222_10000"
     CDS             complement(7931..8617)
                     /locus_tag="VF222_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142795.1"
                     /translation="MESKSPFYIEVYDLNDFARLVCALERAPLPTFSMYLNNEHVFAV
                     QTEFLYGTPVIYYVKFENSNLCNQYLAYRCQGLSETVTLVDIASNPLFVYSPIINVEK
                     LPPVMEKSSRKSKRSSYSKIMLRDLDSLAKVAAYKTIYDEPPLPIFSFGNQKKDTFTL
                     GTSLNFMETDSLTYFYYIIIDKKPEKFLKYSSQKSEKPFFTNYIEEHGYIYIKVIKLL
                     KEHPLVVNDD"
     gene            9174..9536
                     /locus_tag="VF222_10005"
     CDS             9174..9536
                     /locus_tag="VF222_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142796.1"
                     /translation="MNLKELDIAVKIQSSINFLTNAINKKDSDKSEIWQAYSWLEYSI
                     LLIRLKKYNLLDKLPTNELKTNKMQEKVEPELNLIKARNLLWSLDYDDEEQILNSLRE
                     SRDIIKNFLKTKKEVNNK"
     gene            complement(9541..10545)
                     /locus_tag="VF222_10010"
     CDS             complement(9541..10545)
                     /locus_tag="VF222_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX7142797.1"
                     /translation="MPCNIVVGGFFGDEGKGKIVAYLVKKDIISLAARGGVGPNAGHT
                     FSLNGQTFTVRMLPSAAFNVNTNLAIGAGVLVDPKILLNEISTFHAHDRTFVDPHCGI
                     IKEDHILQDSTESFLKDKIGTTGTGTGPANSDRALRKLSLAMDCLEIKNYLNDVSCMI
                     HSDLDKKNNVLLEGTQGTYLSLYHGGYPFVTSKDVTASSVCADVGIGPKYIDEVLVVF
                     KSYVTRVGGGPLENELGKVETQKRGWLEYGSVTGRERRSSPFSIELAKRAIRLNGGTS
                     FALTKLDVLFPECAGVTDYSKLSLPSKRFVEEIEAELGLKAELIGTGAEVDAVIDQRH
                     "
     gene            10645..11106
                     /locus_tag="VF222_10015"
     CDS             10645..11106
                     /locus_tag="VF222_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142798.1"
                     /translation="MSVSISNTEDIIEILGNLQPFLRKPIIKNKLIDFFKSDSEFQVE
                     TIEVILDSLKNINIQKYQDLISSWLEVLVYFENTKINQIIHLYLIGLLKRKELINPLE
                     PTLFYCINKMSMDQKEKLRYCFLETLFMIPNNNNIIYSNLSDETLKFIFKT"
     gene            complement(11133..11381)
                     /locus_tag="VF222_10020"
     CDS             complement(11133..11381)
                     /locus_tag="VF222_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HEX7142799.1"
                     /translation="MTKTCSKCKNTFDENLIDDPGAQKYSSCPSCWNEWTTHAIMVIN
                     ELRLDMSLPEHRKALKKQEKIFFGLEQGEQIQKNPDNS"
     gene            complement(12247..12378)
                     /locus_tag="VF222_10025"
     CDS             complement(12247..12378)
                     /locus_tag="VF222_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142800.1"
                     /translation="MEDKLTIPVCFKPEEIELIETFAKKLGMINSSQAIEKMVSELS"
     gene            complement(12434..12814)
                     /locus_tag="VF222_10030"
     CDS             complement(12434..12814)
                     /locus_tag="VF222_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HEX7142801.1"
                     /translation="MDLGSLKVGSYIIIDNEPCRIVSYDHSKPGKHGSAKARVSAMGV
                     FDGSKHGLVSPVSANVEVPLIDKRSGQVVSITGSLLQIMDLETFEVFETSAVEEDIRD
                     KIAQGKEIEYWKVLDRIKIVRVKA"
     gene            complement(12899..13768)
                     /locus_tag="VF222_10035"
     CDS             complement(12899..13768)
                     /locus_tag="VF222_10035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7142802.1"
                     /translation="MNEISIGIISYNEEKNISNLLNSIKFQYLGKFSLKEIIISDDSQ
                     DETPFLIKEFIKNNSQFVIRLLHHQERRGASAAWNEIFNEASGDYLILYDADVILEKQ
                     TTYNLVNGFGSDIGLCAGNPQPIEVSSIYGRASQFISLWLQNVRKNGLTQFMVMGRGL
                     AIRRELAKEIIISENIIAIDYFLQIKVLELGKKVIYVDNAKIYFKTPKNLEDFLSQVL
                     RANFGHKQLSYFKTKKMSFVTTLKITFKSFLCDPYGGLATIYCYFFIPYYKFHYKHKK
                     ISSKWDIAKSTKN"
     gene            14025..>14645
                     /locus_tag="VF222_10040"
     CDS             14025..>14645
                     /locus_tag="VF222_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142803.1"
                     /translation="MKFGQIPTCENPLLWGVILFGAFLNVILLFSNAYGESINVNDLA
                     NFTILENLNKTTKSHTLLENELDNIKNDQLKEKFVAQIKLIDDKKAVIKVIPISFKNS
                     SLKEEFESPKSNLLSLDRNDTGKSANKTDVLPPPPTISYIENEGEQFNAVNYVSKDGD
                     ITTSQEEDDDESNRNTSQEEDDDESNRNTSQEEDDDESNRNTSQEED"
BASE COUNT         5366 a         2186 c         2189 g         4904 t
ORIGIN      
        1 tcggatttat gagaattcaa atattcttgt cctttgtctt taaggcgatt ttgatagagt
       61 cttaaatctc tatgctcatt aaagaatttt tcgatcattg tacttccatg aatagaagga
      121 tttatcaatt tatcaaaata ggctatagtt aattggacat ctccttgttc tacagattct
      181 ctcgttttag ctaatatttc acttatatcc tttaattgtt gaacaggagg tgaaacttcc
      241 ctagattttc ccatgaaacc tgagcgttta agtgtaccaa cctttgaacc tatttctcta
      301 gagagttcaa aatattcttc tttactaaag aagtttttct tttcctttct tattattaga
      361 cctttcatgg aaagtcttaa taaagcatca tcaacctcaa cttgatccat accggtgatc
      421 cagattattt tacctgaata atcgtagcca tgtctaattg cctctagtac tacaacctca
      481 attattcttc gaaatccttc ttctgctttt gcattttcaa aatctttatc tctgacagca
      541 cgtttggcat aagaaatttc aatttcaatt gctctcttta gtgcctccat cccatcatcg
      601 attctgccat caagcaccaa ataacaagac aggttatacc atgacattcc attattatga
      661 ttgatatcta ttgatttttc acaacattca attgctttta gcaggttatt cagtctataa
      721 tgtgcaagtc ctttaagatc ccaagcgtcg aaattgttta tatctaaatc tagagcatta
      781 tcataacaag aaatggctac accatattct ccaaggtgga aatgagcata tccctttttt
      841 atccatgcat taacaaagtg cggatcaatt ctcaaagata attcaaaaca tctaatagca
      901 tcagtaagtt tctcattagc catatgatta attcccttat tataaagatc aaatgtttga
      961 atatcattaa tattgttagt attatttaat tcagaaggtt ttttatcttt ttcttggaaa
     1021 ttaaatttat ccgttacctt ccttctaaaa aaattttcca tataataaca gatccattca
     1081 cgaagataaa tagattatga tttatttgtt atagtgacca gtacatgtga tcgactttgg
     1141 aaaaaataat aaatcaagaa aaaattatta ttttagcttt accaatatac aattaagtgt
     1201 cagattacta taaaatatta ggaataacaa aggattctaa tcaaattcag ataagaaaag
     1261 cctttcgtga gctagcactg caataccatc ctgataagaa taaaaattct gaagaatcaa
     1321 aagagagatt catggagatt gttcaagcct atgaggtcct atcagatgtg aaagctaggg
     1381 aaagatacga ttttaataca gtcaatgaga aaaagtttca gaaatttaat tggacacctt
     1441 ctgcagattt tgctaatttt tatagttatg agagtcttaa aagagaatac aatgataatc
     1501 aaataagcgg tggtatgtgg gacataagtg aaaaagctaa tgcaggatta tggaaagcga
     1561 catttatctt gttaggatct ctgggcctaa tatctgtctt cattatatta aaaatttaat
     1621 agtgatttag gacaaattca ttcagtgaaa ttctttttaa atgttgatga aaaataaagt
     1681 atttaaaagg aattgaaaat tactcagaat agtaaagttg aaagttttag caataggaaa
     1741 tcgcatcttt gttacaagtt ttcaaatggc tggaatcgag ggaattgatg ttcaaacgcc
     1801 taaagaagct cttgctagaa ttaaccaaat acactctaga aatgaagcag gtttgatttt
     1861 attaagtgat gacattggaa aagaaattcg attagatctg gctaggatac gttctgtaaa
     1921 acctattcct ttgatatttg aactcccttc accgggcagt aaaaaagaaa aggtcgatta
     1981 tagagcattg ttaaagcaaa tcctaggtgt ttaagtatat ttcttattgt atttgttttg
     2041 attacgtttt agaggcagtt tatattactt tactataaga tgaatataag atcagtactt
     2101 agtattgctg gcagtgactc atcttctggc gcgggcatac aagcagattt gaaaactttt
     2161 tcatccttag gaacttatgg atgttctgct attactgcaa taactgttca aaatacaaaa
     2221 gatatcttcg atatatttcc tcttccacat gaaatagtaa tgaaacaaat aaagttcatt
     2281 ctaaaagaca taagaattga ttctataaag ataggaatgg tttatgacga aatactaatt
     2341 aataaaatat ttaatatttt aaaaaatatt aatattccta taatcgtaga tccgattttt
     2401 acttccacga gcggaaaaaa tttaatcaag cctgaagcaa taaaagtttt taaaaaaaaa
     2461 atattatcta ttgcaacaat aactactcca aatatcaatg aggcaaaaga acttactggt
     2521 attgaaataa ataataaaga agattcggtc aagtctttaa ctaagataaa gaaattaggg
     2581 cctcaaaatg tgatcctaaa gggtggacat attttctcag aacgatctat tgacacctta
     2641 attgatgata aattaaacat aatcgaattc tccaatcctc gaatacatat tccagaaaat
     2701 cacggttcag gatgcaattt ctctgcagcc ttgtctgcct acatttcaca agggtatgaa
     2761 atacatatag catgtaaatt cgcaaatgaa tttataaata gggtattgaa aaatattttg
     2821 catataggaa aaggataccc tgtagcggat agtgctatac ttacatcttc ctcatcattt
     2881 aaatttcagg ttatcgagga actatcaata gctattaagg agttagaaaa tttatcaggc
     2941 ttgggaaagt taatcccaga aacacaatct aattttgcat atgcaatacc aaatgctact
     3001 agtatcaagg aagttgctgg gataaaagga agaatagtaa ggatagatga aaaagtgaaa
     3061 gcaagttctt gtattgaatt tggtgcatca cgacatattg cgtcagcagt tatttcctac
     3121 atgactatca accctgcgat gaggacggga tttaatataa aatacgataa aaaaataata
     3181 gagttattac gaacttttat gaaagtcgca gaatatgata gacgattaga acctagaaca
     3241 atcaaggaaa aagaaggtta ttcgatcaag tggggagtta aacaagcgat tttaaaaaac
     3301 aaggctgtaa atggaatctt tcataaagga gacctaggga tagagccgat gataataatt
     3361 tttggacaca aaccagtaga aattcttgaa tatttgagaa aaataataga gtattactaa
     3421 tctaaaagta tattatataa ttaaattaaa cattttagat gcaagcagtt attctggcgg
     3481 gaggcctcgg aaccagacta caaccttata ctttctttat accaaaacct atgttaccat
     3541 tggggaataa accgttgctt gaatatttaa tagaatggat aaaaaaatta agaaacattg
     3601 atgaagtaat tatttgtgtg agttatttgc atcgaaccat cgaagattat tttgaagatg
     3661 gagggaggtt cggaataaaa ataacatatg cccattctga aagaccctta gctactggag
     3721 ggcaattgaa aactgcagaa aaaatgttaa atgacagatt tatttgtctt tacggtgatt
     3781 cggtttatga ttttaattta gaaaaaatgg ttaatggcca tgaaaaatca aaggcttttc
     3841 tttcaatggc attaatgaaa tacaaaacaa gaatgaaata cggtttcatt gatacaaatg
     3901 ataaaggaga tattgttaca gcttggagag aaaaaccaga agttgaaggt ttaataaata
     3961 ttggatgtta tataatggaa aaagaatttc taagaatgat accaaaatct agtacgattg
     4021 gcatggacaa aatagtaaaa gatgtaatta ccaaaaaaaa aaaagttaaa gcgtttacaa
     4081 tagactcagg atttattgat ataggcgata aaagatcata tttagaggct tataaaaaat
     4141 acatgaaaaa attaggtagt atatagaatt atatagcaca aaaacaattt ttctgtttga
     4201 tattaattac taattcgtag aacgtcatct ttttattctg attgaataaa tgatttcata
     4261 gatgattaat actaatagag ataaaaattt atcagttgat gcatcacgta atacaaatct
     4321 caacttttat gttagaaaaa tgtattatca aggatcacta atgataaaca tatgtgatga
     4381 ggaattatta ggaacgaata ttgtaagtga atcgttaaat attaatataa ctaatgattt
     4441 ttttaacgaa tcggtaaatg aaaaagatat atgtaactta ttaaaaagat gttcgatagc
     4501 taatctaata ggaaggagag ttatagagaa gactttgagt ttacgacttg caaaaagaga
     4561 cagtataaaa atagtttcag atattccttt tctaatgata tttaaatttg attaaactat
     4621 tagttagtta ttttatctgt taatttcaaa aatatatatt ttatatgcaa cactttataa
     4681 gattagttaa ggctgagtca tattgagtta ccaacgtaca ttaagaatac aaaaagaacg
     4741 aataggagcc atcattggaa aaaatgggtt agttaaagac gagatcgaaa aaaaatgcaa
     4801 gatagacatt catattgaca gtgaatatgg agatgtaata ttaaaatcta aaaacactga
     4861 ccctttggat atattaaata tcaataaagg attggaagtt attcttgcta tctctaaagg
     4921 tttttctcca gagagagcat ttatcctatt tactgaggaa aacagtttaa gtgtgattga
     4981 cttacgcgac tacgtcggaa aatcagcaaa ttccttagcg agagtaaaaa gtcgcctgat
     5041 aggagaaaaa ggaaaatccc ggagaactat agaggaatta gcagggtctt atatttcaat
     5101 atatgggcat tacgtcggaa taataggaac ttctgaacaa ataactttaa cgactgaagc
     5161 aataacaaga ctatgttcag gtagatcaca taaaaatgta tataacttcc tccaagaaat
     5221 aaggagaaaa gcaaaaattg ataagttaaa attgtgggag tagattggta tcacctaaat
     5281 tttattataa atataaatac attaaggaaa acgtgttaat attgactaat ccacaagatg
     5341 aacgtaaaaa agtctcaaaa atatcttact acaaattaag tactctctta gctattatta
     5401 taactattcc cggaatttca gttctaatgg ctttaagaca ctatgacgtt gacataattt
     5461 atcaaataat tgttagctcg gtagtatttt ttatatgttt aggactttcc tttaaaattt
     5521 ctaatcaact tgtcaaattc ggatgaacaa ctattttttg actccccatc tagtgaacat
     5581 attattttca atattaaatt ggtccaatac ttttcctaca atatgaaata acaaatcatc
     5641 aatagtacgt ggatggtaat aaaatcctgg catggcaggt aatatagaga cccccaattc
     5701 agacaattta accatgtttt ctagatgaat tttagacaaa ggagtttctc gaggaacaac
     5761 aactaatttc ctattttctt taatacaaac actggcagct cgcgaaatca aactatcatc
     5821 taatccattt gctatgctag caagtgtttt catactacag gggataatca ccataccatc
     5881 agtcttaaaa gaaccactag aaatgggggc tgccatattc ttattatcgt accacctggt
     5941 tgatagcttt tgcacataag caatatctaa atttgtttct attttaatcg tcttttctcc
     6001 ccattcgcta ataaccaaat gtgtttccaa tttaagtgaa tttaatgatt ctattaattt
     6061 tataccataa ataactcctg atcccccggt aattgcaata ataatgccca cattttcact
     6121 tagttaaata gatgataagt atttatctaa aaattaattt ttatattcct tcattaacct
     6181 aataaaacat gcttactatt tttggtacaa tagcattaga tacaactaga acatctgcta
     6241 agacggtaaa tagggtcatg ggcggagcag caacatatgc atctatctca tcatcaaaat
     6301 ttacatctac taatattatc ggaataattg gctatgattt tcctcaacag tataaagata
     6361 ttttgaacag acacgtgaag actaaaggaa ttattgtaaa agacgatgaa aaaacttttc
     6421 attatgattc ttcttttgat caaactttga caattcgaac agctaacaaa actgaattaa
     6481 acgttattga aaattttgaa ccatttgttc ccgaagaatt gctcgattcg aaatatattt
     6541 ttttagcaaa caacgatcca gctcaaaaca aaaaaatgca aagcttattt agcgatcctg
     6601 aatttgtggt ttgtgacact atcgaatatt ggatcaacaa taaatatgat caggtaatag
     6661 atatgataca aaaagtaaat ggaatcatag tggatagcca agaagccaga cttttaacaa
     6721 atgaatataa ccttatcaag tgtgctaaaa aaattaaaga attgggccct gaattgataa
     6781 ttataaaaaa aggagagaat ggatctatgc ttttttatga ggatattata tgtgtatatc
     6841 cagctatccc tatagaaaat gtagttgatc caactggagc aggagattgc tatgccggcg
     6901 gttttctggg ttatttgtca aaaaaaggaa agataaattc aaaaactatt agagaagctg
     6961 caatatacgg aaatattatg ggttcttttg caatagaaga ctttggacta aataaaataa
     7021 ttaatatttc ttcttccgat atagagaaaa gattgaaact ctttaaagaa ctagtaatgg
     7081 tctaaaaagt gctgcaaatt agtcagatta ttattctcat attgttatta agaaattata
     7141 aaacaatcat tcatttttat atttcgtgat ttgatatagt atgagtttta aatcattaac
     7201 aggagatgaa gaatgcaata tgtgcctccc aactataaaa taatcagtac cagcttttac
     7261 tgcatcagct atcgaaccac cttgggtgaa aaatcctgga gaatatatct ttatttcgtt
     7321 caaggaggaa aattccttga taacatcaat ttttgaacct cctatgacaa ttccatccgc
     7381 accactcaaa cgagcatagc cataaagtat ttggtacatt ggaattgatt ctctattatc
     7441 accatgctca tcatttgaaa tggaaatttt taatccgtaa ccttcgaaag aagtgggatg
     7501 actcatatag acaagtgaga ttataccgaa ttttattgaa tgggcgtagt ttacaattga
     7561 tttcatttct gttactccaa taaatggatt tactattacc gcatcaaatc ctaatttttg
     7621 tagcttatca acagttactt tatttgtatt tcctatatca ttcagcttga tatctgctat
     7681 actctgcaga tcaaatttat gagcaaggat gttaagttct aatatctgga tatatgaaag
     7741 agataataag agatggaaat ttattttaat tccacaaatg tgagattcac taagtgatat
     7801 tacctcttta catttatcaa gtaagaaact attattataa ttatctaaat cgtaatccaa
     7861 tgctaaaatt atggggcttt tttttttatt tgagcaatta tttatccttg actgataaga
     7921 agacgtaatt tcaatcatca tttaccacga gaggatgttc ttttaagagt tttattactt
     7981 ttatatatat gtaaccatgt tcttcaatat agtttgtgaa aaaaggtttt tctgattttt
     8041 gactagagta tttgagaaat ttctctggtt tcttgtcaat aataatgtag tagaaataag
     8101 ttaaggagtc tgtctccatg aaatttaaag aagttcccaa agtaaatgta tctttttttt
     8161 gatttccaaa agaaaaaatt ggtaatggag gttcatcata aatggtttta taagccgcga
     8221 ctttagcaag actatctagg tctcttaaca taatctttga gtaactactt ctcttacttt
     8281 ttctagaact tttttccatt acagggggta atttttcaac atttataatt ggagaataaa
     8341 caaaaagagg attagaagca atatccacca atgtcactgt ttcagaaaga ccttgacatc
     8401 tataggcaag gtactgatta cacagatttg agttctcaaa ttttacataa taaattacag
     8461 gagtaccata tagaaattca gtttgaacag caaagacatg ttcattattt aaatacatag
     8521 aaaatgtagg taatggagca cgttctaatg cacagaccaa tcttgcaaaa tcattcaggt
     8581 cgtatacttc aatgtaaaat ggtgatttag attccaagca attatcttca aaaaaaaggc
     8641 tattaatatt ttacacttta ttcgaaaagt cataacacaa aaagtataat ataaaaagta
     8701 tctggtatct ttttctctat ttgatattat tcaaagagac tttcaatctc ttctattcga
     8761 gtcatgaaaa ctttaaacgt agtaaactga actgttatac agtgattgtt cttaccccac
     8821 tcagaatttt tataaataag tatcttcctt aattactcta aaatatttaa gctactataa
     8881 ttattttttt tgtttcacat tttccaaacc ttggttgaga aatttagcga aacaagattc
     8941 tatatcgcaa ttgtaataaa atttaattgg atgttattat atacaagttc ccttgcttga
     9001 ggagtgcatt agaagcatat gtattacatg ttattggttt tttttacgaa gagtcaatgt
     9061 cataaaaata ttatattttc tattatagtg attaactcaa cttctctctg cctactttag
     9121 taatcaacat tctccattat tgtttatcta ttataatatc aaaggagatg atagtgaatt
     9181 taaaagaatt agacatagct gtaaagattc aatctagcat taatttttta actaacgcta
     9241 ttaataaaaa ggatagtgat aagtcggaaa tttggcaagc ctatagttgg ttagaataca
     9301 gcattctgct gatccgacta aaaaaataca atctgttaga taaactacca acaaatgaat
     9361 taaaaactaa taaaatgcaa gaaaaagtgg aaccagaact aaatttaata aaggccagga
     9421 atttactttg gagtttagat tacgatgatg aagaacaaat tttaaattct ctcagagagt
     9481 caagagatat tataaaaaat tttttaaaaa ctaaaaagga agtcaataat aaataaatcc
     9541 ttagtgtctt tgatctatta ctgcatctac ttcagctcca gtcccaatta attctgcttt
     9601 aagtccaagt tctgcttcaa tttcttcaac aaaccttttt gaaggtaaag aaagtttaga
     9661 ataatctgta acaccagcac attcaggaaa aagaacatct aatttggtaa gggcaaatga
     9721 cgttccacca ttaagtctta ttgctctttt tgctaactca atactgaatg gagaggatct
     9781 tctttctcgc ccagttacgc ttccatattc aagccaccct ctcttttgag tttctacttt
     9841 acctaattca ttttctaatg gtccacctcc caccctagtg acgtaagatt taaaaacaac
     9901 aagcacttca tcaatatatt tgggacctat tccgacatct gcacaaaccg aagaagcggt
     9961 cacgtctttt gatgttacga aaggatatcc cccatgataa agtgaaagat aagtaccctg
    10021 tgtcccttca agcagaacgt tattcttttt atccaagtca gaatgaatca tacaacttac
    10081 gtcattcaag taatttttta tctctaaaca gtccattgct agagaaagtt ttcgtaatgc
    10141 tctatcagaa ttggctggac ctgttcccgt tccagtagta cctattttgt cttttagaaa
    10201 actttctgtg ctatcctgta gaatatgatc ttcctttata ataccacaat ggggatcaac
    10261 aaaggtccta tcatgagcat gaaatgtgga aatttcattt aaaagtattt taggatctac
    10321 caaaaccccc gcacctatag ctaagttggt attaacatta aaggcggcgc tgggaagcat
    10381 gcgaactgtg aaagtttgtc cgtttaaact aaaagtatgt ccagcattag gacctactcc
    10441 tccccttgct gctaaactaa taatatcctt tttaacaaga taagcaacta tctttccttt
    10501 cccttcatct ccaaagaagc cacctactac tatattacat ggcatgatgt tgattatagt
    10561 aatctatatt taattattaa tataaaagaa aagaaatatt gtaaaatagg aatttaaata
    10621 taagtcgctt acctaattag ttatgtgtct gtaagtatta gtaataccga ggatattata
    10681 gaaatattag gcaatttgca acctttttta agaaaaccaa taatcaaaaa taaattaata
    10741 gattttttta aaagtgattc tgaatttcaa gtagaaacaa tagaggtaat tttagattct
    10801 cttaaaaata taaatattca aaaatatcaa gatttaatat cttcatggtt agaggtatta
    10861 gtatattttg aaaatacaaa aataaatcaa ataatccatc tctacttaat cggattgcta
    10921 aaaagaaaag aattaataaa tccacttgag ccaacattgt tctattgtat taataagatg
    10981 agcatggatc aaaaagagaa attaagatat tgttttttgg aaacattatt tatgatacca
    11041 aacaacaata atataatata ttctaattta agcgatgaaa cattaaaatt tatttttaaa
    11101 acttaatcgc ctgaaaccaa tacaaagaaa aatcaagaat tatcaggatt tttttgaatt
    11161 tgttcgccct gttctaaacc aaaaaaaatc ttctcttgct ttttcaacgc ctttctatgc
    11221 tcaggaagcg acatatctag acggagttca ttaataacca taattgcatg agtagtccat
    11281 tcattccaac atgagggaca agaagaatat ttttgagcac ctggatcatc aatcagattt
    11341 tcatcgaagg tatttttaca ttttgagcaa gttttagtca tatagactat ccaactatta
    11401 aagatatatt acttttgtta ttaaataata ctctaaaata taagagactt aagaactcat
    11461 tgatcctacg aaaaaattta ctcatttgta atttaattgg taaacctgaa atttatataa
    11521 atagtcacaa tgataaaacg atagaatcac ttgcaaataa gtaaaatttt acattgacga
    11581 ttataattct gccctgatat ttagaacaac ttgagagtca atatcattaa ctgaagattt
    11641 tcaattagtt tagaaataag actgtaatta tagatgatga ttacaaattt aattagtcaa
    11701 attaaataat gaagtcagtc tctatattaa ttattatgta taaacaaaga aattaagacc
    11761 tcatggagga aactaaaaca acaatatcaa tatatctatt aaatttaatt caaatatttc
    11821 ttctatttcc acaattgagg taatagatta taagaaacaa tgtattggta tgtcaggcta
    11881 ttaataaatt ttaataaggt ttattaatat aatctattgt gtttttttat ttttcttata
    11941 ttgatgattt ttttttatat ggtctcgcat ttgttccata ccgaaaaact taagcttgca
    12001 gctgttgcaa gtataacatt taccagaacc atgtattact tgttcatgtt gcattaattc
    12061 ttctattttt cgaaatcttt tattacatat tggacaatga aattttttcc aaatatttat
    12121 taaaaaattc aatgatctca atgatctcac agaatgtaca tttaatataa agttaaaaga
    12181 aaactttttg aaggaccagt atttgatttc aattcatatt agaagaaaaa aatgttggaa
    12241 atgtaactaa gacagttcag ataccatttt ttcaattgct tgactagaat ttatcatgcc
    12301 taattttttt gcaaaagtct ctatcaattc tatttcctca ggtttaaaac aaaccgggat
    12361 tgttagttta tcttccataa caattcaaac caaaagaagg tttttatgtg tttccaaaaa
    12421 ttaaatcaaa cgactatgct ttaacgcgta caattttaat tctgtctaga actttccaat
    12481 attcaatttc ctttccttga gcaattttat cccgaatgtc ttcctctact gcagaggttt
    12541 caaatacctc aaaggtttcg agatccatga tttgaaggag agacccagtt attgaaacaa
    12601 cctgaccact tctcttatca ataagaggga cttcaacatt ggcggaaaca ggagaaacaa
    12661 gaccgtgttt agatccatca aaaactccca tagcagaaac acgtgcttta gctgaaccat
    12721 gttttcctgg tttgctatgg tcatatgaaa caattctaca aggttcatta tcgataataa
    12781 tataagatcc gacttttaaa ctgccaagat ccattggttt actcataata attgaaagaa
    12841 aaagaatatt gatatatatt atttctgttt taaaataaat ttgtctaagt actctttttt
    12901 aattcttagt acttttagca atatcccatt tagatgagat ttttttatgt ttataatgaa
    12961 atttatagta cggaatgaaa aaataacaat atatggtagc gagaccaccg tagggatcac
    13021 ataagaaaga tttaaaagta atctttagag tagtaacgaa agacattttc tttgttttaa
    13081 aatatgataa ttgtttatga ccaaaattag ctctcaaaac ttgagacaaa aaatcttcta
    13141 aattttttgg tgttttgaaa tatatctttg cgttatcaac ataaataact ttttttccca
    13201 attccaatac ttttatttga agaaaataat caattgctat tatattctcc gaaattatta
    13261 tttctttagc taactctcgt ctaatagcta aacctctacc cattaccata aattgtgtta
    13321 gcccattttt tcttacgttt tgcaaccaga gtgaaataaa ttgagaggct cttccatata
    13381 tactggaaac ttctattggt tgaggattcc cagcacaaag tccaatgtct gaaccaaatc
    13441 cattgacaag attatatgtg gtttgttttt ctaatattac atcggcatca tataagatca
    13501 aataatcgcc tgatgcctca ttaaaaattt cattccatgc agcagatgca cctcttcttt
    13561 cttgatgatg taaaagtctt attacgaatt gagaattatt ttttataaat tctttgatta
    13621 aaaaaggtgt ttcatcttgg gaatcatctg atataataat ctctttaaga gaaaatttac
    13681 ctaaatattg aaatttaata gaatttaata gattagaaat atttttttct tcgttgtaag
    13741 aaataatccc gatagatatt tcattcatta tacatttttc gaagaaaata cttttataaa
    13801 tattagcatt aatattataa ttgcatataa tagcatatgt tacaatttgt ctgctcgatc
    13861 aaacatataa caaactttgt gttatcaaca agaacatgtt gagaagcaaa cttagactaa
    13921 taataatagc accaagcttt gctatcatac attctttaac aaaaatttta tatctaattt
    13981 aatcattcgg ttaaaaaata aaatgacttc taataattta taatatgaaa tttggacaaa
    14041 tccctacgtg cgaaaatccc ttattatggg gtgttatttt atttggagct ttccttaatg
    14101 tcatactgct tttttcaaat gcatatggtg aatcaattaa cgttaatgac ctagctaatt
    14161 ttactatact agaaaatcta aataaaacaa caaaaagtca tactctgcta gaaaatgaac
    14221 tagacaatat taaaaatgac caacttaagg aaaaatttgt tgctcagatt aaactaattg
    14281 atgataaaaa agctgtcatt aaagtaattc caatatcttt taaaaactca tctttaaaag
    14341 aggaatttga gtctcctaaa tccaatttgc tctctttaga tcgaaatgat acagggaaat
    14401 cagcaaacaa aacagatgtt ttaccaccac ctccaaccat tagctatatt gaaaacgaag
    14461 gtgagcaatt caatgctgtg aattacgtat ccaaagatgg tgacattact actagccagg
    14521 aagaggatga tgacgaaagc aatagaaata ctagccagga agaggatgat gacgaaagca
    14581 atagaaatac tagccaggaa gaggatgatg acgaaagcaa tagaaatact agccaggaag
    14641 aggat
//
LOCUS       DASVEV010000224         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1074656, whole genome shotgun sequence.
ACCESSION   DASVEV010000224 DASVEV010000000
VERSION     DASVEV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074656"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF222_10045"
     CDS             <1..230
                     /locus_tag="VF222_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142804.1"
                     /translation="TVLKISQIEVNEEKQQWWFVEPYDDIFQEYNSIDGYPAHIIDKV
                     EEIISKKIGNKQVKVTVFAYKNTIGSYDILN"
     gene            complement(345..881)
                     /locus_tag="VF222_10050"
     CDS             complement(345..881)
                     /locus_tag="VF222_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142805.1"
                     /translation="MFFLITLVLSSQSIALCEAHIKEKVQEWIDKNKNIKIQFDYSPE
                     KPIIDIFTELQFSVQDLETKQHLKDFDARVVVTKDQRLFKFENITVTNGDFSIKYIFP
                     DDGTHKVLLRVNTNNSIVLASFSVFVPHQSPPSLLNPFPNNQGQPYTVGQWISITIII
                     VIPIAVLFSVVLIIKKKV"
     gene            complement(1016..1723)
                     /locus_tag="VF222_10055"
     CDS             complement(1016..1723)
                     /locus_tag="VF222_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HEX7142806.1"
                     /translation="MQEVLAVFSGIIIFGLLHGINPSHGWTIAVLYSLQTKTPLLSSI
                     LSSSILAMAHFLSSVVVVLAFILFSTYIDIPQNYLNYAAAIALAILAYMFWKEKPEDL
                     VNNQHGHLHQFSEQVKHNHSHWHKDEGYHIHFHTHQIRILPSLTTLAISALILGFAHE
                     EEFVILSLAAAGVNPILLMIVYALSVSVALIGITILSVKVITTIQDRIIHYTKYLPKV
                     SAGILAIMAISFGLDLV"
     gene            1942..>2583
                     /locus_tag="VF222_10060"
     CDS             1942..>2583
                     /locus_tag="VF222_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142807.1"
                     /translation="MVNNSSDDKYKNKPDKDLSAIKLGLKPNINQFLLLIIVNAFVGA
                     MIGLEQTVIPILGKEEFDLESNALILSFIASFGLVKAVLNLFAGNISDKFGRKNVLVL
                     GWLFGIPVPFILLFAPNWNWILFANVLLGINQGLAWSMTVNMKIDLVGTQQRGLALGL
                     NEFAGYVSVALVGFLTGYIAAVYGLKPYPFYLGIIFPIIGFLISFLLVKDTKEF"
BASE COUNT          927 a          350 c          409 g          897 t
ORIGIN      
        1 tgacagtatt gaaaatttct caaatagaag ttaatgaaga aaagcagcaa tggtggtttg
       61 tggaaccata tgatgacata tttcaagaat ataatagtat tgatggttat cctgctcata
      121 taatagataa agtagaagaa ataatttcaa aaaaaattgg aaataagcaa gtaaaagtca
      181 ctgtttttgc ttataaaaat actataggca gttatgacat attaaattga caattagcaa
      241 ctttcgaaat ttttttcatt tcaatgtttc ctgataacca gaaaattttg gtttccaacg
      301 ctaatcaaat atatttaaat aaaacacaca ataagccatg aatattaaac cttctttttt
      361 attatgagga ctactgaaaa taatacagcg atcggaatta caattataat agtgatacta
      421 atccattgtc caacagtata tggttgacct tggttgtttg gaaatggatt taatagagat
      481 ggaggggatt gatgcggaac aaatacacta aatgatgcta atacgatgga attgttggta
      541 ttcacacgta atagaacttt atgtgttcca tcatctggaa atatatattt tatagaaaaa
      601 tctccatttg taacggtaat attttcaaat ttaaatagtc tttgatcctt tgtaacgact
      661 accctggcat caaaatcttt tagatgctgt tttgtttcta gatcttgaac gctaaactgc
      721 agttcggtaa aaatatcaat aataggtttt tcaggtgagt aatcaaattg aatcttgatg
      781 tttttatttt tatcaatcca ttcctggacc ttttctttaa tatgagcctc acataatgct
      841 atactttgac ttgataatac aagagtgatt aaaaagaaca aaattttaga tatattaatg
      901 ttgaatattc aaacatcttg tccaatatta ttgtagtgtt actattttaa ttctaaccca
      961 cgtatactat aattatcaaa aaattatatt ttaatttcgt agataaataa taattttaaa
     1021 ctaaatctaa tccgaatgaa atagccataa tagccaatat gcctgcactt acttttggaa
     1081 gatatttggt ataatgaatg attctatcct ggatagtagt aattacttta acagataaaa
     1141 ttgtaatacc tattaaagca acagatactg atagcgcata tacaatcatt aacaatattg
     1201 gatttacgcc tgctgccgct aaggaaagta ttacaaattc ttcttcatgt gcaaacccta
     1261 gtattaaagc agaaattgct aatgttgtga gtgatggtaa tattcttatt tgatgtgtat
     1321 gaaaatgtat atgatatcct tcatctttat gccaatgact atgattatgc ttaacttgct
     1381 ctgaaaactg atgcaaatga ccatgttgat tatttactaa atcctctggt ttttctttcc
     1441 aaaacatata agccaatatt gcaagtgcaa ttgctgcagc atagttgaga taattctgag
     1501 gtatatcgat ataggtgcta aataaaataa atgctaatac tacaactacg gaagaaagaa
     1561 aatgggccat agcaagtatg ctggaactta aaatgctact taagagcggt gttttagttt
     1621 gaagtgaata taaaactgct attgtccatc catgcgatgg atttatacca tgtaacaaac
     1681 caaaaatgat tataccagaa aagacggcta atacttcttg cacttatgga tactgaatat
     1741 ttatatctat tttaatcctt tttgaatgca aatacaaaat tctaattatt tatcataaga
     1801 ttgagtcttg aaacaaataa taacagaaaa aataagtaga tcttacagat aaaaataaga
     1861 aataaatgat ttggatttta tctaaggtta taacaataaa tagaaataac aaacagatct
     1921 tttgataggt ctatcataaa agtggttaat aatagtagtg acgacaaata caaaaataaa
     1981 cctgataaag atttgagtgc tattaaactg ggtctaaaac ctaacattaa ccaattcctt
     2041 ttgttaataa tagttaatgc atttgttggc gcaatgattg gactagaaca aacagtaatt
     2101 cccatcttag gaaaagaaga atttgatttg gagtctaatg cattaatatt gtcttttatc
     2161 gctagttttg gcttagttaa agccgtcctc aacctcttcg caggtaatat ttctgataaa
     2221 tttgggagaa aaaacgtatt agttttaggg tggttatttg gaataccggt cccttttatt
     2281 ttattatttg ctccaaactg gaattggatt ttatttgcta atgtattact tggaataaac
     2341 caaggtttgg cttggtcaat gactgtcaat atgaaaattg atttagttgg aacacaacaa
     2401 cgaggattgg cattgggact taatgaattt gctggatatg tatcagttgc tttagtaggt
     2461 tttttaacag gttatattgc cgccgtttat ggattaaaac catatccatt ttaccttgga
     2521 ataatatttc ccataattgg atttttaatt tcgttcttat tagttaaaga cacaaaagaa
     2581 ttt
//
LOCUS       DASVEV010000225         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1074862, whole genome shotgun sequence.
ACCESSION   DASVEV010000225 DASVEV010000000
VERSION     DASVEV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074862"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..119
                     /locus_tag="VF222_10065"
     tRNA            45..119
                     /locus_tag="VF222_10065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Met,seq:cat)
     gene            220..474
                     /locus_tag="VF222_10070"
     CDS             220..474
                     /locus_tag="VF222_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142808.1"
                     /translation="MVTKNIKLGSVDATGVPFGCLNSQERGLDIAEYLEEEEIPILIE
                     NTPKLQKRAFLDCLYDSGSRPEEFLRLSNLDCKIDTMAQY"
     gene            1301..1978
                     /locus_tag="VF222_10075"
     CDS             1301..1978
                     /locus_tag="VF222_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7142809.1"
                     /translation="MSNRLIPKSKRFVLLVFIFIIISFLVTFKATNNIDNLISTFFFH
                     FAGDPSMDMFMIIVSSIGDLFTMLIIGIILTIIKRTRKIGLIFLISIVVLSISIMYLK
                     PIINRASPLEQYIPQYHIPNNFTIENDSMVPFAIDLSFPSNHVARDTAFIIIIGYWFR
                     LFKNNTLVGKLLWTFPVLIGLSRLYLLHNYFFDIVGGGLYGMIIAIILVKVLKLNEPF
                     STNKFNK"
BASE COUNT          771 a          325 c          340 g          691 t
ORIGIN      
        1 atatataaca attgaaataa caggaatgaa acgctgctga gcttgccgga gtggctcagc
       61 ttggttagag cgttcgactc ataatcgaaa ggtcgcgggc tcgaatcccg cctccggcat
      121 tgatttctcc gttcgagtcc caatctccct agaagataaa aagaataaca aaacaaattc
      181 taaacagaca atagttcaat agtacaagaa aaaataatga tggtaacgaa aaatataaag
      241 ttgggttctg ttgatgctac gggtgttcca tttgggtgtt tgaatagcca agagagagga
      301 ttggatattg ctgagtatct ggaggaagaa gaaattccaa tactgataga gaatactcca
      361 aaactacaga aaagagcatt tttagattgc ttatatgact ctggtagtag acccgaagaa
      421 tttcttagac tctctaactt agattgcaaa attgatacaa tggcgcaata ttgattttaa
      481 gaagaaaaac agagaaggaa ggattagact tgcttcattt gccaaattat tgcaacaatg
      541 gttagatatc caccctctaa agcatcaaaa acaatttcca ttatgaataa gccaggtaac
      601 tcacttcaat aaccaacctc taggattgaa aggcgctgaa aagataattg aagaagcact
      661 agcaaaagcg aaattggaga aacacaaaag gctctatctg ttacgacatt ctagagccac
      721 tcacttatgc aaatggttga cagaagctca gatgtttgtt ttctttggtt ggcaacaagg
      781 aacaaaagtc atgaatatta tcactttatt atatcactaa cctaaaatta tcacataatt
      841 tgtgaatagt gtattgtcac aaaataattg ttaacatgtt taacgaaaat agtattaatg
      901 aatctttctc taaaccattt acaagtcatg agattaatga ctttaatttt tatcacaaat
      961 ctttatctaa aaattatcac cttatatgga atatcactga ttcattaatg aatcctacaa
     1021 aaaagaggat tcgaacagag cattctaatc ccgcctccgg cattgagtct ttacttttaa
     1081 gccgcgtcaa catttaagct aaaataaatt agactcatca gagattttaa actctaaaag
     1141 gagaacactc ggaattaagc aaatacctca aatttctaat ttaggtttca aatgatggat
     1201 ctatctcgga ggtaaaaaga caggaatatt agatacaaag aaataaaaaa agatgtgagg
     1261 taatattata tatatataat taaaatagtg gatactgaaa atgagtaaca ggttgatacc
     1321 taaaagtaaa agatttgtat tattggtatt tatatttatt attatatcat ttttagttac
     1381 ttttaaagca acaaataaca ttgataattt aatatctact tttttctttc actttgcagg
     1441 agatcctagt atggatatgt ttatgattat tgtctcttca attggtgact tatttactat
     1501 gttgataatc ggaataattt taactataat aaaaagaacg agaaaaattg gcctgatttt
     1561 tctaatatca attgtagttc tatctatatc aataatgtat cttaaaccca ttataaaccg
     1621 ggcatctcca ctcgaacaat atattccaca atatcatatt cctaataact ttactatcga
     1681 aaacgatagc atggtacctt ttgcaataga tctctccttt ccatcaaatc atgtagcacg
     1741 agatactgct ttcataataa ttattggata ttggtttaga ttgtttaaaa ataatacatt
     1801 ggttggaaaa ttattatgga cttttcctgt attgatagga ttgagtagat tatatcttct
     1861 tcataattat ttttttgaca tagtaggagg gggactatat ggtatgataa tagcaataat
     1921 cctcgttaag gtattgaaat tgaacgagcc atttagcaca aataaattta acaaatagaa
     1981 tcaaccatat gcattttttg ttcagtcaga atttattata atccatccaa aatgattttt
     2041 atcatgtata tatctaattt ctctcatcct agtttttatt tttcgttgtc tcaattgaga
     2101 agtaaattct aattgaagac taaggcc
//
LOCUS       DASVEV010000226         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1464116, whole genome shotgun sequence.
ACCESSION   DASVEV010000226 DASVEV010000000
VERSION     DASVEV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464116"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..2021
                     /locus_tag="VF222_10080"
     CDS             441..2021
                     /locus_tag="VF222_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142810.1"
                     /translation="MNDITNNNEKDDKYNNSKNKIDVKSTTSYQNKIPKDAWITLAIL
                     SLIGLIIVYGETMIIPAIPDLVEDFNIPYNTTAWILTAYLIAGAVATPIVGKLSDIYG
                     KKRILLLVMIIYTAGTSFGIISNDISTMIIVRTIQGIGMSVFPIAFTIIREKFPENKL
                     SIAQGILTSIFAAGGVIGLSLGATTIEYFGWRSTFISISPLALILTLIIIRFILVKEQ
                     EKSNNNNTIPLRKSIDIKGAIALTITISSFLISLTLLGNSNSTDFNNTNNNTINSSLI
                     ILFSVLSIISLFIFVLVQRRTHIPLIDINLLKDRILLPTNILLMTIGISMFMIYQTIS
                     ILIRNPPPSGFGGSAIDAANVQLPFMIISLIISVLSGFLISRFGNIKPTIIGSVISVI
                     GFSSLFMYHSTEFEIIINLSIIAGGLSLAEVGGFNITLVSVSPKIGGISMGITVLLFL
                     IGMSIGPAISGIYLETFQSPIMLNKISYSFPSAFSYDMIFLTSALLSFIFLILAILTA
                     KRIISTKVISKHSKDDSN"
     gene            2375..3139
                     /locus_tag="VF222_10085"
     CDS             2375..3139
                     /locus_tag="VF222_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142811.1"
                     /translation="MFSALKFQHSNEKYFQLNTMSNVLFFSFLIMLFLFSCYSHFVYA
                     EEIITIIPGASDSSRYRFFDITEYPISPGKEIKWYNADNIFHNIKIMSKDGKTIIAES
                     ENIKPKEYFNYEFKEEGEYLFQSSKYPWMKGKIIVTYDIKTIKESLGNDIDLYISWTP
                     STIKSGEKALFKIIFIDKKSNKNQEHIDYSFIIKDSTADEVLYKNSLTHSAWGIEPAS
                     YKFNLTGNFVGEVSIQGILFQPINPQYVEFEIESAQ"
     gene            complement(3598..4200)
                     /locus_tag="VF222_10090"
     CDS             complement(3598..4200)
                     /locus_tag="VF222_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142812.1"
                     /translation="MTIVSLTVISLIYTMAFAQSTNQTSTLQQSPQQQQQSSFCKLTE
                     SDMLGPFYKEGAPFKQRLGEGVEGERLIITGKVMDMRCQPLKDAILDVWQANSTGKYD
                     NEGFTLRGKVKTNNDGTYLIDTIVPREYATGDITRPGHIHLKVGAPNQPTLTTQLYFE
                     GEPYLSDLEDKSLVMKLTEENGTKKANFDFVIEHYDEYDK"
     gene            complement(4371..4580)
                     /locus_tag="VF222_10095"
     CDS             complement(4371..4580)
                     /locus_tag="VF222_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142813.1"
                     /translation="MSHITDANKWREESSKGLLCTKFQCYNKPRNQCSECSIYYCNEH
                     VTSHIHTDTFQHLQREQERNIDNTR"
     gene            5279..>5885
                     /locus_tag="VF222_10100"
     CDS             5279..>5885
                     /locus_tag="VF222_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7142814.1"
                     /translation="MNRTLITYNILAAIILFSAVTAITMQPISINAQIPPLQQQPSTQ
                     PLAISPSCGQVITQNVILTSNLNCAESDGLIVDAGNIVVDLNGHTISGPDVDSDTQTS
                     SKTGIVIPNVNNVVVQDGSIEGFQAGILTTGSQNVEVKGMVVKNNQIGLYSTGSSIVN
                     AHLSIIMNNQIGIAEHSTQQSTIEDNILFQNTLAGITLVNSD"
BASE COUNT         2144 a          829 c          793 g         2119 t
ORIGIN      
        1 atttatgtaa aattctctac gaaacatctt taagttttga aatgtaaaat aattttatag
       61 tgtgaagaaa aagttctata catgctatac atgaataata aaatacaata ttggttggaa
      121 taataagtta catattctta agagctgcaa ttaatgcgat aataaaatat gtttgtttca
      181 taagattctt ttaatattat tcttttttaa acattgtctt ttgaaataaa aaaactaaat
      241 acacgtaatt atagaatcct accacaaaat tccactccga tctgaaagta ctttttttaa
      301 aactcattta actcttaacc tgatagcagt ataatggata ttgcaattga acaatttgtt
      361 ataggataga agtttgtaag gtttttatgg atagaaaata ctatctgcaa tatactaatt
      421 aatatcgtta ggtctaactc atgaatgata taactaacaa taatgaaaaa gatgacaaat
      481 ataacaatag taagaataag attgatgtaa aatctacaac atcatatcaa aataaaatac
      541 ctaaagatgc atggattaca ttagcgatac taagcttgat tgggcttatt attgtgtacg
      601 gtgaaaccat gattatacct gctattccag atttagtcga agattttaat ataccatata
      661 atactactgc atggattctt accgcatatt taattgcagg agcggtagca actcctattg
      721 taggaaaatt atctgatatt tatgggaaaa agagaatttt actacttgtt atgattattt
      781 atacagcagg tacttctttt ggaataattt ctaatgatat ttcaacaatg atcatagtgc
      841 gtacaattca aggaataggt atgtctgtat tccctattgc ttttaccata attcgtgaga
      901 aatttccaga aaacaaatta tctattgctc aaggaatact tacctccata ttcgcagcag
      961 gaggagttat aggtttatct ttaggagcaa caacaatcga atattttggt tggcgttcta
     1021 cttttatatc tatatcaccg cttgcattga ttctcacgtt aattattatc cgatttattc
     1081 ttgttaaaga acaagaaaaa agtaataata ataatactat accattaaga aaaagtatag
     1141 atattaaagg tgccatagct ttaacaatta ctatctcttc cttcctgatt tcactcacat
     1201 tgttaggaaa ttctaattct acagatttta ataatactaa taacaatact ataaactcaa
     1261 gtttaattat attattttct gtattatcta ttatatcatt atttatcttt gtactagttc
     1321 aacgaaggac acatattcct ctaatagata taaatttgtt aaaagacaga atacttcttc
     1381 caacaaatat tctgttaatg actattggta tttcaatgtt catgatttat caaactatat
     1441 ctatacttat aaggaatcct cctccatcgg gatttggagg aagtgcaatt gatgcagcaa
     1501 atgtacagct tccttttatg ataatctctt taataatttc tgttttatca ggatttttga
     1561 tatcaaggtt tggtaatata aaacctacaa taataggtag tgtcattagt gttattggtt
     1621 tttcaagtct ttttatgtat cattccactg aatttgaaat aataataaat ttgtctataa
     1681 tagcaggagg attatcatta gctgaggtag gaggttttaa cattacatta gtttctgtat
     1741 cgcccaagat aggtggaata tcgatgggta tcacagttct tttatttctt ataggaatgt
     1801 ctattggtcc ggccatatct ggaatatatc ttgaaacatt tcaatcccct attatgttaa
     1861 acaagattag ttattctttt ccatctgcgt tttcatatga tatgatcttt ttgacatccg
     1921 cattactgtc ttttatattt ttaattcttg ctatactaac tgcaaaaaga attatatcta
     1981 caaaagtaat ttctaaacac tctaaagatg attccaacta aaggtaattt ttaacaataa
     2041 atgttaggga aactataata aaactctttg aaagtaataa gctgaagaaa ggtaaaaaat
     2101 atgttagata tataataaat aagtcacaat aatatacaaa attattgtct attttactct
     2161 taattttttt taataatcat cttttaagaa ttattattaa tatttttaat actaaatatt
     2221 gataccctac attatcgtca taagaattat taaaaatcaa aagggggata tcgatctgga
     2281 ttaatgacaa cccagaatcc taacgataat ttaatttatg atagatcaaa cctaaagtat
     2341 aattaacata tcatgcttta agaaataata tctaatgttc tcagctctca aatttcagca
     2401 tagcaatgaa aagtattttc aactaaacac aatgagtaat gtgttattct tttcattttt
     2461 aattatgctt tttttatttt cttgctattc gcattttgtt tatgcagaag aaataataac
     2521 aataatcccc ggggcaagtg attcgagtag atatagattt tttgatataa ctgaatatcc
     2581 tattagccca ggaaaagaaa taaaatggta taatgctgat aacatttttc acaacatcaa
     2641 gattatgtct aaggatggaa aaactataat tgcagaatca gaaaatatca aaccaaaaga
     2701 atattttaat tatgaattta aagaagaagg agaatatcta tttcagtcat caaaatatcc
     2761 atggatgaaa ggaaaaatca ttgttacata tgatatcaaa acaataaagg agagtttggg
     2821 aaatgatatt gatctatata taagttggac accttctact attaaatctg gcgaaaaagc
     2881 cctctttaaa ataatattta tagataaaaa aagtaataaa aatcaagaac atatagatta
     2941 ttcattcatt ataaaagatt ctacagcaga tgaagtttta tataaaaatt ctttaacaca
     3001 ttctgcttgg ggaattgaac ctgcatccta taaatttaat ttaactggca atttcgtcgg
     3061 agaagtaagt attcaaggaa tactattcca acctatcaac ccccaatatg ttgaatttga
     3121 aatagaatcg gcacaataag aaataagaaa ttttcttttt taaacctgta ggttgaaata
     3181 tctatatcta caaatgcctt tatgaaaagt gggaaacctt cttgaacctt ctttgtactt
     3241 ttctcgagtt cactgtattt gatagtaatg ataaattaag tagtcttttg acataagttt
     3301 gctattcatt cctttcttat cattatccat aatttactaa atttgacaag tttgtcggag
     3361 aaaagacaag aataataaca tctctaatat tttgattgct tttgcttatt attaacaaat
     3421 aattttacta ttttaaaaat aatattattg ttaactttac aagaaagaaa aaacaattat
     3481 caaaattata ttatttatat aaaactcgat attatcagaa atttcaatca cttgtgattt
     3541 aaatttaaat tattaaaatt aatgtctttc tccattgtct aaaaattgta aataaattta
     3601 tttatcatat tcatcataat gttcaattac aaaatcaaaa tttgcttttt tagttccgtt
     3661 ctcctctgta agtttcatta ccaatgattt atcttccaag tcactcaaat aaggttctcc
     3721 ttcaaagtat aattgagtag ttagtgttgg ttgatttgga gctccaactt ttaagtgtat
     3781 atgacctgga cgagttatat ctcctgtagc atattctcta ggaactatgg tatctataag
     3841 atacgttcca tcattatttg ttttaacctt gcctctaagt gtaaatcctt cattgtcata
     3901 ttttccagtt gaattagctt gccagacatc taatatagca tctttcagtg gttggcatct
     3961 catatccata acctttcccg ttattattag tctttctcct tctactcctt cacccagtct
     4021 ttgcttaaat ggagcacctt ccttatagaa gggacctaac atatcagatt cagttagttt
     4081 acaaaatgaa ctttgttgtt gttgttgcgg tgattgttgc aatgttgaag tttgattagt
     4141 agattgtgca aaagccattg tataaattaa agatatgact gtcaatgaca caattgtcat
     4201 caagccaaaa acaagatttt tcattataag gttaataaat aatatgctat ataataattg
     4261 aagatatttt ttattaaaac attacattga aaaggtaaat tatatttata catcgagtac
     4321 tttagtaata cttattgtta ccaacaaaga aattgataat aaaaaagaat ctatctagta
     4381 ttgtctatat ttcgttcttg ttccctctgt aaatgttgga aagtatcagt gtgaatatgt
     4441 gatgttacat gttcattgca ataataaatt gaacattcag aacattgatt tcttggcttg
     4501 ttatagcatt gaaattttgt acaaagtaac cccttcgagg attcttccct ccatttatta
     4561 gcatcggtaa tatgactcat ataataaaat tttataagat aatatatttg actttagatg
     4621 aaaatttcta ttcaagtatt acctacaata atattttact aggttcagtt gtgtgttcga
     4681 ttgtgataca tgacaagtta ttatctcaag gctttgtaat aataattctc atctaaatta
     4741 tttgatatat cttttgatat tatatctatt ttattattag agaagaagga gaaataaaaa
     4801 actattttat aaatataaat tatatccctg ttaaatttta atttttttgg attgaattta
     4861 tattactaca catacggata attgtgtaat cgttatttta tgacagactt gggttatttg
     4921 atgattttag gtattccgca tttatctcca taaatattga gatatttttt tactgattgc
     4981 tattaataat ccaattccaa tatattgtcc aattacaact tggtaaggaa gaaattttaa
     5041 ataattagaa aagaacataa tcagtagaaa aatgtcatca atatttgtag atacaaatgc
     5101 agatatgcct aaaactatca aatagccaaa gccctaccat aaaataaact actaattcat
     5161 tttccattga ggtagtaata taaattaaat ttcaagtaaa aggaataata aaataattga
     5221 aaaaataatg gttctttatg gattaacata aatatgtcag tatcaaacca tctactaaat
     5281 gaatagaaca ctaattactt ataatatatt ggcagcaatt atcttattct ctgccgttac
     5341 agcaataaca atgcaaccca tatctataaa tgctcaaatt ccaccattac aacaacaacc
     5401 atcaacacaa ccattagcca taagtccctc atgcggtcaa gttataacac aaaatgttat
     5461 attaacttca aacttgaatt gtgcagaaag tgatggtctt atagtagatg caggaaatat
     5521 tgttgtagat cttaacggac atacaatttc tggtccagat gtagatagcg atacacagac
     5581 ttcaagtaaa acaggaattg tgataccaaa tgtaaacaat gttgtagtgc aagatggtag
     5641 tattgaagga tttcaagccg gcatactaac gacaggttct caaaatgttg aagtaaaagg
     5701 aatggttgtt aaaaataatc aaataggact atacagtact ggttccagta tagttaatgc
     5761 acatctgtcc ataattatga ataaccaaat tggtattgca gaacattcta cacagcaaag
     5821 tactattgaa gataacatac ttttccaaaa tactttagca ggaatcacac tagtcaactc
     5881 agata
//
LOCUS       DASVEV010000227         8736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1660186, whole genome shotgun sequence.
ACCESSION   DASVEV010000227 DASVEV010000000
VERSION     DASVEV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660186"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..775
                     /locus_tag="VF222_10105"
     CDS             245..775
                     /locus_tag="VF222_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142815.1"
                     /translation="MLKQKKYIYSGILCFSLTIGIGHYFSTYVQSTEENSIKNTTITK
                     IENIEPVYIATSGECLSSKQVESHPYVVIEDKCIEQATIKSIGKVLNNLTFLNTIMDN
                     NTIFGQGYGKIVSEDGQMIGWRSIDGNVFDSGHPGYRGIIYFNSTMDEKFSSLNNTIG
                     VFRSDSGTNKSIWLLE"
     gene            complement(1067..1462)
                     /locus_tag="VF222_10110"
     CDS             complement(1067..1462)
                     /locus_tag="VF222_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142816.1"
                     /translation="MVIVCNNTINTDNKKRILVVDDEPDICLVLKIVFEKTGLIVDYY
                     YNPILALDEFKSNFYDLIILDIQMPDINGFQLYREIKRKDINAKICFLTASETIFDTA
                     YKFSPVYTFIRKPIQNNDLILIINGLLNN"
     gene            complement(1796..3349)
                     /locus_tag="VF222_10115"
     CDS             complement(1796..3349)
                     /locus_tag="VF222_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7142817.1"
                     /translation="MLKYYTNNCEFIRSKLGSKIVVFVFILIILVITSFIIFSYYQSQ
                     MTYLGNSINIAGKNRFLTSTLMFDISKYLIEDSNKDVSKINSAINQLEFNILVLKEGG
                     KAASIDLMPLQGEFLNDWNKVYQKWISLKTIIANNIIKPSDENIGSIDKLTQKKLETE
                     ALSLVNLSNILVTKLGEYVKSNSENLLFNQRVFIILDIAVIAAFVLYLTRKIVKPILS
                     LTSAISDVNRGTLNLIAQSKGNNDDELSVIGNSFNYMTNFINKIKKQDELINELEKAN
                     QELKYKDQLKNEFINVAAHEIKTPIQPIIALSELLQQEGINNIEKNKELLNIILRNSK
                     RLKQLTEDVLDVARIETGSFFLNKEKFNLKEMITDVLKEYEQTIQNKNYIKFFLEFND
                     NKEIIIEADRNRLSQVIHNLLHNAIKFTKEGSITIIVERKKDNTNDKYDEILVSIKDT
                     GTGIHPEVMPKLFTKFVTKSPIAGTGLGLFISKSIIEMHGGKIWATNNKEKDKGVGST
                     FTFSLPLKQ"
     gene            4328..4864
                     /locus_tag="VF222_10120"
     CDS             4328..4864
                     /locus_tag="VF222_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142818.1"
                     /translation="MNFQIIITNNNKTNKIIKIRIELNLGDITCFMKPHSDFVCDTIV
                     RGILPKRYNGLESPTVYVLVTDNKFDFYNITEIADKKYKILDRALKHIIVRRIFTIHQ
                     LARFLLIDLERDLEKYKSKLVVITGDFFLSDPQITKQDKDWLYPQMIKAIKKVAAASI
                     ILVYSPTNLLNLMNYGIG"
     gene            complement(5117..5716)
                     /locus_tag="VF222_10125"
     CDS             complement(5117..5716)
                     /locus_tag="VF222_10125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7142819.1"
                     /translation="MTIPIVKTAIESDEAGVIDVLKLAFVADPATRWVFPEPQEYLSH
                     FYSFAKAFGGKAFASQSAHYFENYSGAALWLPPNVYPDVDQLVVLLQSSGSEDAKKDG
                     PKVFEKMSSYHPNESHWYLPLLGVDPLYHGKRLGSALMQHAIVACDLDNKLAYLESSN
                     PKNIQFYERHGFELLGEIQVNSSPPIFPMLRKPRKLQHD"
     gene            complement(6299..7153)
                     /locus_tag="VF222_10130"
     CDS             complement(6299..7153)
                     /locus_tag="VF222_10130"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX7142820.1"
                     /translation="MITGIIAISIFFIADYYSYDFLLISTVFAQPIPDNSGVMMYKYP
                     NIGHNVYNPLAISSGGLDYYSKYQNTGDNKSKEYFINTANWLVNNTKDKEGGKYSLWE
                     FDFTWPWYNQLTPPYYSGYAQAVGIDVLARAYDLTNNTMYLDEAKKAFSAFLVDYDNG
                     GVMTIEDNNGDLMFFHELGKPGFQKTYILNGHTGALAHIWHFYEITNSPLVKTIFDKG
                     INYLKHNLWKYDTGSWTRYDLKPIGQIENLATLGYHKVHIEHLNALYRITGEPILKEY
                     ADKFNTYM"
     gene            7471..7602
                     /locus_tag="VF222_10135"
     CDS             7471..7602
                     /locus_tag="VF222_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142821.1"
                     /translation="MEKYKETPNTQKMNDQVIIDNIIEELDKKGYSYKQKEKSFVKR"
     gene            complement(8002..8613)
                     /locus_tag="VF222_10140"
     CDS             complement(8002..8613)
                     /locus_tag="VF222_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142822.1"
                     /translation="MSTKDDKINEANTNRTKFNTDNTKTSTNFNDRTNNTLNQQQDTI
                     NKTLDNTLHNVKRTTDEATREIPRYTQRIAEYQEQTIKTIKDIASDFIEAEKEVIGSF
                     QTQVDKNSGGVWDLYNPQRIAENYAVMVNNFTSYLLNNSNLINNALASNMRVYNTALE
                     QTRDNLKAFAKTNTNYVKAVDRQNSDLNTRSSSNNSNERTTRY"
BASE COUNT         2966 a         1223 c         1253 g         3294 t
ORIGIN      
        1 agaaacaggt gattcttgat ttaatagttc ttgcttaccc atatattgcc aatacataat
       61 cacaaacaaa ctattagaag ctatgtgtaa tgggtttcta ccaaaagggt gtttggtcgc
      121 cttttagacg ttgtggagga aactatattc ggtggaatta tctagaagtc tcgatacaat
      181 tcttttgtca atacaagtaa aaaatattta taaatatcga caaccaatca aataaagaaa
      241 atatgtgctg aaacaaaaga agtatattta cagtggtatt ctttgttttt cattaactat
      301 aggtattgga cattattttt caacctatgt tcaatcaacg gaagagaatt ccattaaaaa
      361 tactacaata accaaaatag aaaatattga acctgtctac attgcaacaa gtggcgaatg
      421 tttgagttct aaacaagttg agtctcatcc gtatgttgtt atagaagata aatgtattga
      481 acaagcaaca ataaagtcta tcggtaaagt actaaacaat cttacatttt taaatacaat
      541 aatggacaat aatactattt ttggacaagg atatggaaag atagtttctg aagatggtca
      601 aatgattgga tggaggtcta ttgatggaaa tgtgtttgat tcagggcatc ctggttacag
      661 gggcataata tattttaatt ctaccatgga tgaaaagttt tcctccctta ataataccat
      721 tggggtattc agaagcgatt ctggtacaaa taaatctata tggctattgg aatagactaa
      781 tcaagacttc atttaaacca caccaataga tattaattta aaaatttttg aaatgtaatg
      841 ttaagttaaa gttatactac tatctagatg tagcttttat ttttttatta gaacttattt
      901 tagatatatt catagtatat tgaattctaa agaagtatct caaggagaga gtaataccga
      961 tagcgattct aaaagaggag tccaatgtaa agatagtagt atcatgtatt tagcttttat
     1021 tattagaaac ttagttttaa atttttaagt tcataaataa aattaattag ttatttaata
     1081 aaccattgat tattagaatt aaatcattat tttgtatagg ttttctgata aaagtataca
     1141 caggagaaaa cttgtatgca gtatcaaata tggtttcact agcagtgaga aaacatattt
     1201 ttgcgtttat atcctttctt tttatttctc tatataattg aaatccatta atatctggca
     1261 tttgtatatc tagtattatt aaatcataaa aattagattt gaattcatca agtgctagta
     1321 taggattata atagtaatct actataagtc cagttttttc gaaaactatc tttaatacta
     1381 aacaaatatc tggctcatca tcaactacta atattcgttt tttattgtca gtgttaatag
     1441 tattattgca tacaattact attaattatt tgttataata atttaattac acctatatta
     1501 gtgaataact cattttataa gttaaatata ctattgtgta tagtagaaac taaaaaaaag
     1561 ggaattaaca agaaatgatg gaaaaggcca ttgtatagta taaatattaa aaaatataat
     1621 gacttaaata tttgaatagg tttttaatat aagaaatcct ttgtttccct ttatagtatc
     1681 ttattctcgg gtttggtgga atgaacgttg aaatttcaat acaattctat taactattta
     1741 attttatatg ttattttaca taaatttttt attaaaatga atttaaaaaa tattgttatt
     1801 gtttgagtgg caggctgaat gtaaatgttg aacctacacc tttatctttt tccttattat
     1861 tggtagccca tatctttcca ccatgcattt caattatact cttggaaata aatagcccca
     1921 aacctgttcc tgctataggg gatttagtaa cgaattttgt aaaaagtttt ggcattactt
     1981 ctggatgtat tccagttcct gtatctttta tactaacaag aatttcatca tatttgtcat
     2041 ttgtattatc tttttttctt tcaacaataa tagtaatact accttccttt gtaaacttga
     2101 tggcattgtg taacaagtta tgaataactt gactaagcct attcctatct gcctctataa
     2161 ttatttcttt gttatcatta aattctaaaa agaattttat atagttttta ttttgtatcg
     2221 tttgttcata ttctttcaag acatctgtta tcatttcctt gagattgaat ttttctttat
     2281 ttaaaaagaa agaaccagtt tcaattcttg caacatctaa tacatcttct gtgagctgct
     2341 taagtcgttt ggaatttctg agaataatat ttagcaattc cttatttttt tctatattgt
     2401 taattccttc ttgctgaagt aattcagaaa gagcaattat tggttgaatg ggtgttttta
     2461 tttcatgtgc tgctacattt atgaattcat tctttaactg gtctttatat tttagttctt
     2521 gatttgcttt ttcgagttcg tttattaatt catcttgttt tttgattttg tttataaagt
     2581 tagtcatata attgaaagaa ttacctataa ctgaaagttc atcatcatta ttccctttgc
     2641 tttgagctat taaattaagt gtccctctat ttacatccga tattgcagaa gttaaggaaa
     2701 gtatgggttt tactatcttt ctggtaaggt aaagtacaaa agcagcaata actgcaatat
     2761 ctaaaattat aaagactctt tgattaaaca ataaattctc tgaattactt tttacatatt
     2821 cacctaattt tgtaactaat atatttgata aatttactaa agatagtgct tctgtttcta
     2881 attttttttg tgttaatttg tctattgaac ctatattttc atcgcttggt ttgattatat
     2941 tgtttgctat gattgtttta agtgaaatcc atttctgata tactttattc cagtcattta
     3001 aaaattctcc ctgtaatggc attaaatcga tacttgctgc ttttccgcct tcttttaata
     3061 ccaaaatgtt gaattccaat tgatttattg cagagttgat tttagataca tctttattac
     3121 tatcctcaat caaatatttt gatatatcaa acatcagagt agatgtaaga aaccgattct
     3181 ttcctgcaat atttattgaa tttcccaaat atgtcatttg tgattgataa taagaaaata
     3241 taataaaact agtaataaca agaataatca aaataaaaac aaatactaca atttttgaac
     3301 ctaactttga acgaataaat tcacaattgt tagtataata ctttaacatt gactatttca
     3361 agttcaaaat tttaaataat gacgtgttgt tgttctagtt gaaattgtgt tgttattgct
     3421 gaatgtgttt ctgttaaatt atcattatca tcttttctat cttgtttttt agttgacata
     3481 tactaaattg tgatttatag cacttaaggt gaggtaaaca atggtgtaga ttccccttat
     3541 tctatattaa taataacgtt tcttgattaa attatataaa atgaattgga aaattaatga
     3601 ataaccttag taggatttat tatatctgta ccatattttt attcctcaat cgacttgcag
     3661 aatcctattc aatccgtcag ttatgcagca gagcttgaat ctttctgttt cttttcttct
     3721 gctttaggct gaataagaaa atttatagaa ttaataaagt tatgacattt gttttcacat
     3781 tctttataat atcttttatt ataaaaatta atattccaat agtagtaatt ttctttgttt
     3841 atatcttact ttcttctcaa atagatattt gctaagatag tattactata ttacattagt
     3901 gaattacatt agttgatgtt tattgtgctt tcaaggtact aatttttatt ttttaaatta
     3961 attgtaaaaa ggatttgttg aattatttgt tacaaactct aatatcagtt ctattctata
     4021 atcttatttt tagtttgttg gttaatcacc tctcttatta ctaatttaat aacttgtttc
     4081 tcctattcat attattatag tcaatagtat ctttctgact gagaatcagt gtctaaatat
     4141 ttctataaat accaaatatg tagcaaactt tacaaatata ttctatctct ctccctctct
     4201 tcaaccagct tgctacaata aatataatga aattttgatt catggtagtt gatattatca
     4261 gataacaaaa tagagtcttt acctaaattt atatcagctc aaaaactctt agaagataaa
     4321 tttctacttg aattttcaaa taataataac aaataataac aaaactaaca aaataataaa
     4381 aataaggatt gaactaaact taggtgacat tacttgtttt atgaaaccac attcagattt
     4441 tgtttgtgat actattgtaa gaggtatatt accaaaacgt tataatggtc ttgaatctcc
     4501 tactgtatat gtattagtaa ctgataacaa atttgacttt tacaatataa cagaaatagc
     4561 tgataaaaaa tataagatat tagacagggc attaaaacat atcatagtaa gaaggatttt
     4621 cacaatacat caattggctc gttttttgtt aatagattta gaaagagatt tagaaaaata
     4681 caagtcaaaa ttggtagtta taacaggtga cttttttctt tcagacccac aaattacaaa
     4741 acaagacaaa gactggttat atcctcaaat gattaaagca atcaaaaagg ttgctgcagc
     4801 ttctatcatt cttgtatatt ctcccacaaa cttgctaaat ttaatgaatt atggaatagg
     4861 ataagaaagg atgaatagat aacttgtcaa aatactactt aatttattat tatcaaatat
     4921 ggtgaacccg agaaaactac ggacccgtcg ggagttgaat agacacggtt tcaatgtcca
     4981 gtgctattca aatttaaaca tcgatgattg ttaactttta ggaatgtttc attattgaga
     5041 tggtaagttt atgtgaaata tatttatgaa agcgatatta ttagcccatt cttatactag
     5101 aatttctggt taggaattaa tcgtgttgta acttacgagg tttacgcaac attggaaaga
     5161 tgggtggtga tgagtttacc tgaatttcgc caagcaattc aaatccatgt cgttcgtaga
     5221 actgaatatt ctttggatta gatgattcga ggtaggctaa tttgttatcc agatcgcatg
     5281 caactattgc atgctgcatt aatgcagagc ctaggcgttt cccatggtaa agtggatcta
     5341 ctccaagaag tggcaagtac caatgtgact cattcggatg atagctactc attttctcaa
     5401 atacttttgg gccgtccttc ttggcatctt cagacccact actttgaagt aatacaacca
     5461 actggtctac atcagggtaa acgttcggag gaagccaaag agctgcacca gaatagtttt
     5521 caaaatagtg ggcgctctga gatgcaaatg cttttcctcc aaaagccttg gcaaagctat
     5581 agaaatgtga aagatattcc tgtggttctg ggaataccca acgtgtggcc ggatcagcta
     5641 caaatgctag ttttaataca tcaataacac cagcctcatc tgattctata gcagttttaa
     5701 caattggtat tgtcatctaa atatccctct ctcttattga tgtaagtaat attttaaagt
     5761 tttatagaat tgacttaaaa tataattgca atattgtttg atcaaaaggt atcgaacgaa
     5821 aaattattta ctagtctttg actaccaaaa taaaaactaa ttcatggaca cattcgtcat
     5881 attgaattga tattttttct atcatcttgt gtaattatgc tcacatctct acgcttatta
     5941 tctgcaaaca atactataga tgattatact tattcaaatt attaattatt aaatgctttt
     6001 tagagcgcaa atacctataa attaattcct tatactctat agaatagttc cacatatttc
     6061 cctttcatac aatttcatcg ggctcagagg ttttacggtc atatttgtct gtaatcctaa
     6121 ttaaaaaatt aaataacaaa ttcattgggc tcagagggat ttgaaccctt ctataaagtg
     6181 ggaccagtta ctttttaaga ataagttatt ttatataaga tacatttaat cgttatttat
     6241 gtcaagaata atagagagag attataatta atcaacaggc caaagaaatt agtggctgtt
     6301 acatatatgt attaaatttg tctgcatatt cttttagtat cggttcgccg gtaatacgat
     6361 ataatgcatt taggtgctcg atatgaactt tatgatatcc cagggtagct agattttcta
     6421 tttgaccaat aggtttcaga tcataacgcg tccaacttcc agtatcatac ttccataaat
     6481 tatgttttag atagtttatt cctttgtcaa atattgtttt tactagagga ctattagtta
     6541 tctcataaaa atgccatata tgtgctaatg cacccgtgtg gccatttaaa atatatgtct
     6601 tttgaaatcc aggcttaccc aattcatgaa aaaacattaa atcgccatta ttatcttcta
     6661 tagtcatcac cccgccatta tcataatcaa ctagaaatgc tgaaaatgcc ttctttgctt
     6721 catctaagta cattgtatta ttggtcaaat cataagcacg ggctaaaacg tctatcccta
     6781 ctgcttgtgc atagccggaa tagtatggag gagttaattg gttataccat ggccatgtaa
     6841 aatcaaattc ccaaagagag tattttcctc cttctttatc cttcgtatta ttgacaagcc
     6901 aattagcagt attaataaaa tattctttcg atttattatc tccagtattt tgatatttgc
     6961 tataatagtc taatccccca ctagatatgg ctaatggatt gtatacatta tgtccaatgt
     7021 tagggtattt gtacatcatt acgccactat tatctggaat gggctgtgca aatactgttg
     7081 agattagtaa aaaatcatag gaataataat ctgctatgaa aaatatactt attgcaatga
     7141 tgccagtaat cataatataa aaaataggcg atgatatgat catattgttc tgtttatttt
     7201 ataaagcgta tatttattta attttgtcgt atttttttag ataaaaatgt tataatgtat
     7261 aactaaaata gaaaattata taaaacctac agacctacaa aggaaaacat agaatttaga
     7321 ttcaaaattt aaaacaaaat ctaattcata tactatatgc tagctcaaat ttaaaaatcc
     7381 aacagtaaca tcttaaaaaa tcaactcaac attcctacag aattccctag agttaaacgt
     7441 caccaaaaat tagaattaat agattggact ttggagaaat ataaagaaac acctaataca
     7501 caaaagatga acgaccaagt aataatagac aatatcatcg aggaactgga taaaaagggg
     7561 tatagctata aacaaaaaga aaaaagcttt gttaaaagat gaagcatcaa tttatagatt
     7621 agaggaaaat actatcgatt atatactttt cataattcgt gaatggacag gtaaatggta
     7681 ttaaaataaa aataaaagaa agcaattcaa attatttatt tattattact atcaaatgta
     7741 gtgggcccta gaaaactacg ggcccgtcgg agtaataccg atagcggttc taaaagagga
     7801 gtctaatatc atatattctg ctatcttgta tttagctttt tagtaactta atttgtttga
     7861 aatggatgga atagtatatc ctgaatttaa gattggcctt tcatgtctta aataaaataa
     7921 aataaaataa aataaaataa aataaatata gattgattag gtacaataat tcaaaatttc
     7981 agattgtata ctaagttaaa attaatatct agttgttctt tcgttgctat tgttactgct
     8041 gctgcgtgta tttaagtctg aattttgtct atcaactgct tttacgtaat tggtgtttgt
     8101 ttttgcgaaa gcctttagat tatcacgtgt ttgttcgagt gctgtattgt atactctcat
     8161 atttgatgct agagcattat ttattaaatt gctattattc aaaaggtaac ttgtaaagtt
     8221 atttaccatt actgcgtaat tctctgcgat tctttgtgga ttatacaaat cccatacacc
     8281 accactattt ttatctactt gtgtttgaaa agagcctatt acttcttttt ctgcttctat
     8341 aaagtcagaa gcaatatctt ttatagtttt aatagtttgt tcttgatatt cagcaatacg
     8401 ttgtgtatat cgtggaatct cccgtgtagc ttcatcagtt gttcttttta cattatgtaa
     8461 agtgttgtct agtgtcttgt ttattgtatc ttgttgttga tttaaagtat tatttgttct
     8521 gtcattaaaa ttggtactag tcttggtatt gtctgtgttg aatttagttc tgtttgtgtt
     8581 ggcttcatta attttatcat cttttgttga catattaaca aatacgttac aaggtatata
     8641 aaaaaatgta aagatatctg tgcataatca tgttctgtat atagaaatca tcttaattta
     8701 tacaatgtga ctagtaaagt ttttttttgc tgattc
//
LOCUS       DASVEV010000228         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_884419, whole genome shotgun sequence.
ACCESSION   DASVEV010000228 DASVEV010000000
VERSION     DASVEV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884419"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            825..1055
                     /locus_tag="VF222_10145"
     CDS             825..1055
                     /locus_tag="VF222_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142823.1"
                     /translation="MNFTSKFIETALGISFSKVEEVKNNRIKELLEIKSKIKEDPVLV
                     ENWFDNEINKIRQVTENNSSSIDIESLINKIK"
     gene            1550..1864
                     /locus_tag="VF222_10150"
     CDS             1550..1864
                     /locus_tag="VF222_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142824.1"
                     /translation="MKSKIHSTIVIFSILAFVLISVAGMPPTSPFENVLAQGEAESAG
                     QQGENQTMEDPQQAANQTMERAIQSANETGEDVAEVGSKVTEGAKDLVGNIGDKLQDL
                     GK"
     gene            2565..2969
                     /locus_tag="VF222_10155"
     CDS             2565..2969
                     /locus_tag="VF222_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142825.1"
                     /translation="MKIIVISSLLILLLLSSTFLLPSLTPVKGQESITINCSFENAKL
                     TKISHSLEKDSEELRENYKRYGDSALNALNSLPEEMAEIIKTNLETEILMEKIKTNPI
                     IEYRNCSPLDAVEIELITTLKQGEEKWNEILE"
     gene            3555..3725
                     /locus_tag="VF222_10160"
     CDS             3555..3725
                     /locus_tag="VF222_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142826.1"
                     /translation="MQKSRVFTFGLLMSLLMMLTAPFANLNILSNAMAQGYNENYYED
                     RKYSKYPTDDKK"
     gene            3995..4255
                     /locus_tag="VF222_10165"
     CDS             3995..4255
                     /locus_tag="VF222_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142827.1"
                     /translation="MARESSLYTERFNLVDEPQEYYTIISLTDTIEFIYRIHKKKMID
                     KELWQRWEYHAKGMMTIPKFRKVWNATKQFHTRNFVNFMDAL"
     gene            4331..4543
                     /locus_tag="VF222_10170"
     CDS             4331..4543
                     /locus_tag="VF222_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142828.1"
                     /translation="MNYGQEDKYKFFYVINMSDVNIIKFLINTNSIGENRCIPLSEVD
                     YQKLKDFGDVLVTIIRNNTKPPLGIP"
     gene            complement(4710..4922)
                     /locus_tag="VF222_10175"
     CDS             complement(4710..4922)
                     /locus_tag="VF222_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142829.1"
                     /translation="MQYLQIKYPLPNREEDENNNNNKDDTDNDITGRTNFMLDNSDAI
                     LKWIEFLELVSDEEFDRIINDNSKVK"
BASE COUNT         2128 a          744 c          730 g         1956 t
ORIGIN      
        1 tactcccctt ctcgttgcaa taattcaata tattgacgcc tagaaataga caaaatcgat
       61 ttaaaaaagt ataaacgaaa ttaaattaaa ttaaatataa aaattttttg tgtagaagaa
      121 gagttggatt agttgtttag gctatgacaa ttaaatatat caatgtatta aataatttat
      181 ttcatctttg ttattaccaa tcgctgttat tgactccttc atagcctcac tcaatttgct
      241 cctaattcca gtatcatgag gttatgataa tttactaata acaacaacaa gtcacaaaat
      301 tatctcaaat tattaaataa aatattggac atatataata cacaataaca acaacaatct
      361 gtcaagaaag gaatcataat cagccatagc catcctatat gtcattgctc cggtctacta
      421 ttaaggctat ggtatgaaaa attatttcca actattaaat attataacat tgatctaaat
      481 acaatcttat atgcaaatat aagagttcat ctatgccata gccttctatt attgctttga
      541 tgcccataat agtgcgttat ggttattatt acttttacaa aattattttt aattttaata
      601 tgattccata acttgaattt ctcattaaat tatagtcagg ataatttact ctatgattat
      661 tattataaaa aaaataataa aagtctttta caagttttaa atcatttgtt atttataatt
      721 tattaatgat ttggacaata atatccgtag ttataggaat aataatattg gttctgttat
      781 tgagattttt atttggaata atatagaaag aggatgtaat aaggatgaat tttacttcaa
      841 aatttattga gactgctctt ggtatttcat tctccaaggt agaggaagtc aaaaataaca
      901 gaattaaaga attgcttgaa ataaagtcaa agattaaaga ggatcctgta ttagtcgaaa
      961 attggtttga taatgaaatt aataagataa gacaggtaac agaaaataat tcttcttcta
     1021 ttgatattga atctctaata aataagataa aataagttgt taccatctct taaaaacaaa
     1081 atattcctta tcaaatggaa attcatcaat acatcctagt cttatctaga caacaatgac
     1141 aacagcagat ctctagatgt atctataaac tcactatagg tgcttgcttg tttgttcagg
     1201 ctatgacaat aattaattta tctcatatta ttaaatagaa tatttcttta ttgatattac
     1261 caaccaccaa taattttatt cgtcgttgga cttgtgcata catagccttc ttgttaataa
     1321 tttggctcat aagacgaatg ctatgatttt attattacga tgcaataaaa taaaaccaat
     1381 aaatttatca agttctagat gtataaaaaa tcactactac taccagcaga gtactatagt
     1441 tgtaatccaa tcactccatc acgccatggc atccaacatc atcattactg tttgctccta
     1501 aatctaaggc tatggctttt aataatagtt ttatttaact aaaaatgata tgaaatcaaa
     1561 aatccattca acaatagtta ttttctctat tcttgcattt gttttaattt cagtagccgg
     1621 aatgccacca acgtccccat ttgaaaatgt attagcacaa ggtgaagcgg agagtgcagg
     1681 acaacaagga gaaaatcaaa ccatggagga tcctcaacaa gctgctaacc aaactatgga
     1741 aagggcaatt caatctgcca atgagactgg agaagatgtt gctgaagttg gctctaaagt
     1801 cactgaggga gcaaaagacc tggttgggaa cattggagat aaattacaag atttgggaaa
     1861 atagatacat aactttatat tttaaaaaaa attaataatt tcgatttgat tgttttctta
     1921 cactgcatct aatcttttcc ataatataat taattgtata ggttcgtatt gtaaacaata
     1981 ttgtgacctt taggccctct ggtgtttctg taagtataat aagaggaaaa agtagaaaag
     2041 atttctgaaa agaattgggt aaagtttaat ttaatttaat ttaattctgt gtttatagta
     2101 gtagactata aatctatgtg tcctatttgt aagaagaaat tcattgaaca ttcagaattg
     2161 caggacaaaa tatgtagaat gatcacaata aagcaatttg caaataactc tccaggtttt
     2221 gatgttcagt ttagaccttt tttcgaggac taaaatatct caaaatttta taaagtataa
     2281 tttcgctttt ttagaataga ccagtgaatg caagtaatgg gttcgaatac cagttcctct
     2341 atacaaatca tattttaaaa tagtaaagta atattgcatt aagattcttt aattaatatt
     2401 ttataacaat tatatcgata atgaaattta tttaattgtt tagttaatat aaataataaa
     2461 aaatattatt ctgtagtaat tactaataaa atattgtaga aattatattc tttttaattt
     2521 tttaaaaatt tatgtaatat aatatttata gattgcttca ctatatgaaa attatagtca
     2581 tatcaagttt actaatacta ttattacttt catctacatt cttgctacct tctttaactc
     2641 cagttaaagg acaagaaagc attactatca attgttcatt tgaaaatgcc aaattgacta
     2701 aaataagtca ttctcttgaa aaagatagtg aagaattgag agagaattat aaacgatatg
     2761 gtgattctgc tttaaatgct ttaaactcat tacctgaaga aatggcagaa ataattaaaa
     2821 caaatttaga aacagaaatt cttatggaaa aaataaaaac aaatccaatt atagaatata
     2881 gaaattgttc tcctcttgat gctgttgaaa tagaattaat tactacatta aagcaaggtg
     2941 aagaaaaatg gaatgaaata ttggaataat caagaagcag atttaagcaa aaatgaaatc
     3001 aaacagataa aggaatacat catagtacag attaataatg aatgagatgt ataaatatta
     3061 atcgtgaatc ttaaatttat tactatatta tatcctataa aaaaaggatt taattataaa
     3121 tttgaagttt aagttgtata aatctaatta gttacaataa ttagaaataa tattaaaacc
     3181 ccgttaggta tagttaaact attatctttt taattaaccg aatattttaa tactagaaaa
     3241 tagagccaac atttgatgga taggggttcg aatccctttc catgtttatc aaatgatagt
     3301 tttatacaac aattaaatat attatagtgg ttgattaatt gaaaagttta taacaatatg
     3361 ttaaagaaac aaaacaatat caaatcacac aaattaagtt cctattatta ttcatgatag
     3421 aatactagat aaaaattaga aaatggaaat cacaataaac attcattaat taatttcact
     3481 aatctaatct agcaatacta tgttaccaag tgtacttaag aacttcagtt atattttaaa
     3541 tgtttaaaat tgacatgcaa aagtctcgag tattcacttt tggactttta atgagtttac
     3601 ttatgatgtt aacagcacct ttcgctaact tgaacattct ttcaaatgca atggctcaag
     3661 gatacaacga aaattattat gaagatcgaa agtatagcaa atacccaaca gacgataaaa
     3721 aatagaatgt atagaacaat ctaaagagtg cagggaatag taatagtcta attctttatt
     3781 atattgtttg tttgtttatt attataatgg ctacaacatt atttccaaca gaatttatct
     3841 atcttcttac tgtttattat acaggtgcat taattgtctc gattgccgta ttgttaatta
     3901 ctattaaaaa aatcgctttg aaggtgagta tcaattatat gcaagaattc ttgattctcg
     3961 aactaaactt caaaatacgg atgtcttcac taaaatggct agggagagtt cactttatac
     4021 cgagagattt aacttagttg atgaaccaca agaatactat actataatat ccttaacaga
     4081 tacaattgaa tttatttata gaattcacaa aaagaaaatg attgataagg aactatggca
     4141 aaggtgggaa tatcatgcaa aaggtatgat gactattcca aagtttagaa aagtctggaa
     4201 tgcaacaaag caatttcata ctcgtaattt tgttaatttt atggatgcct tataacttgc
     4261 aatttctcat ttcttttttt tgtctcctct aattctaaag ggttttattt aataagttag
     4321 cttgaagacc atgaattatg ggcaagaaga taaatacaag tttttttatg ttattaacat
     4381 gagtgacgta aatataataa aattcttaat aaatacaaat agtataggag aaaatagatg
     4441 cataccttta tcagaagtcg actatcaaaa actaaaagat tttggagatg ttcttgttac
     4501 aataattaga aataatacaa agccacctct aggaatacca tagataataa aataaaccaa
     4561 aacattcttt tactataatt tttttaaaca gcaaattggt taacagaata aaacaaaaaa
     4621 acactaatac tattacttca ttcctgctac atataaaaga tccagataac tacaaaatga
     4681 tatatttctt ttaacctata gaatttgaac tatttaactt ttgaattatc attaataatt
     4741 ctatcgaatt cttcatctga aacaagttct agaaattcaa tccacttgag tatggcatct
     4801 gaattatcaa gcataaagtt ggttcttcct gttatatcat tgtcagtatc atccttgtta
     4861 ttattattat tttcatcttc ctctctgttt ggtaaaggat atttaatttg aagatattgc
     4921 attagttttt ctccttttct catatacaat tagtagtata agatgattca taaaacaatt
     4981 gtgttgataa atctttattt acctttgaca aaataagaac ctcgtttcaa gatgaaaaat
     5041 tctgatctaa ttttagaatc ttgtacaacc tgtgaaacaa gaatttaaat caggattttc
     5101 aggaaagaca tgacaaataa gattgatata cttttacttg aagaaaaacg taaatcattg
     5161 aaaagtaatc aatcgtatta ctctacaaaa aaacaaattt tatcaattta gacctcgagt
     5221 cttatttttc ttaggtttat atttctaatt aaatgataca tctaaaaaaa aattcatttt
     5281 aattacgctc tttttgcgat agttttagca cttaagtgtt aataaatacc aattatatta
     5341 ccagttatag tattccttaa taatgtctag gcactcattt ttttaaatgt ctaccgctgc
     5401 caaaaatgaa tcagtagaac tatttcatag tctttcaaaa tagcttgcta tatacattaa
     5461 ccaggcatct atcaaaatat ataaaattta ataatgagtt caacctcaac atttattcgt
     5521 ctgatttatt agtggacagg accattctat gttttctg
//
LOCUS       DASVEV010000229         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_885159, whole genome shotgun sequence.
ACCESSION   DASVEV010000229 DASVEV010000000
VERSION     DASVEV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885159"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..884)
                     /locus_tag="VF222_10180"
     CDS             complement(522..884)
                     /locus_tag="VF222_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142830.1"
                     /translation="MENKGENTQIPNKSLSVTGNKEPMDKVEEKSKSFTDKIVNKISH
                     IDSVKEIENALDKSSNVKFITFGLFALIGFIVIIYAILVAVVADTVIQSANVFFVVTI
                     ISISTLVGTLVYQIRPPK"
     gene            complement(2035..2283)
                     /locus_tag="VF222_10185"
     CDS             complement(2035..2283)
                     /locus_tag="VF222_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142831.1"
                     /translation="MKFPNFFKIGLIGRMTRGFQKVKMYNKVVCPICIKYTKNHVINH
                     YNSKYLCLKCYKSLERRGELNETVGGGLAELVQTKDDI"
     gene            complement(2668..2877)
                     /locus_tag="VF222_10190"
     CDS             complement(2668..2877)
                     /locus_tag="VF222_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142832.1"
                     /translation="MISNEGTRRDEFTITPSNPPASDQKPALVPGGADSIQRSMIQQI
                     PSIEKQSQLGFFFRLPLPSLALENK"
     gene            complement(2908..3102)
                     /locus_tag="VF222_10195"
     CDS             complement(2908..3102)
                     /locus_tag="VF222_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142833.1"
                     /translation="MFKNYDKIRMILRGHNHSYARMNVVPGTDFVYVTVGNGGANPHQ
                     DSGESGSPPKQYVRLNGALH"
     gene            complement(3219..3821)
                     /locus_tag="VF222_10200"
     CDS             complement(3219..3821)
                     /locus_tag="VF222_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142834.1"
                     /translation="MQYSVSVNNIQGYETVQQNHCQGQIKDKEIKTCLTTIYDSTKPL
                     SIPSSQPIGGADQSATAQPPAPLSPPQTEGGGAEGGTGGGSGNLLPSTPFNFTGNGDF
                     RDNTNTDENMAANNPEVVLILGDFSYNGIAAQWWSKNMDALNNFNVIRALGNHDDPND
                     DFLNLWPINGGKWEFIYKLSNVPFVAFDTENNDHQLFIHY"
     gene            3816..3974
                     /locus_tag="VF222_10205"
     CDS             3816..3974
                     /locus_tag="VF222_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142835.1"
                     /translation="MHQFGLAVLRRGKRGWNCRGSQQIYYKIRCGLLPPLSFIVFVAI
                     MKTDLRLC"
     gene            complement(4577..4774)
                     /locus_tag="VF222_10210"
     CDS             complement(4577..4774)
                     /locus_tag="VF222_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142836.1"
                     /translation="MVSTKLLKLKVEMDIDIPTDLIESKNRLKLIEEGIIKSICKGLY
                     EEGISFHIRNFNFELNDNVKN"
BASE COUNT         1981 a          759 c          792 g         2196 t
ORIGIN      
        1 aagaaaaaac aatagattaa ttgaagtaca aattagaaat aaaaacttga aatgtataac
       61 tgatgataaa tttgattgta ttcatgttgg gtttactttg gcattaccag aagttagaaa
      121 agctttgagt aaatgaatat ataaaaaaga ttatctatta ttattataaa agaggccatc
      181 aaattagggt cttattatac aaggaactaa atctaaatac attgacatat tatatttttg
      241 ataaaaaatt taaactctgg atgaattctg aaaaacatta aaaaaaaaca taaaatgcaa
      301 accatatagt atggtaactt gttcttatat tatacaatga aatttctttg tatatgaagt
      361 aggattaatt gctttataat ttgtccattt gtaacgattc ttaagtatgg ccactttaat
      421 ctagtattat atcatcttta atagccttta gctaaatata attcgttata gtatatctaa
      481 aagaataaat tgtagttttt catcatattc tcataaaata actactttgg aggacgaatt
      541 tgatatacaa gtgttccaac tagagtagaa atacttatta ttgtaactac aaaaaataca
      601 tttgcagatt gaattactgt atctgcgaca accgcaacta atatggcata gattattact
      661 ataaaaccaa ttaaagcaaa taacccaaag gttataaact ttacattaga tgatttatct
      721 aatgcatttt caatttcctt tacgctgtca atatgtgata tcttgtttac gattttgtca
      781 gtaaaggact ttgatttttc ttcgactttg tccatgggtt ccttattgcc tgttacagaa
      841 agtgatttat ttggtatctg cgtattctcc cctttatttt ccataaatat attttaacat
      901 tatacttaat acaattataa acattgtgtt taaaaattgt atagaagagt tatattgtta
      961 atccgtatcg aatatatcta taaataaata aagttattca aatatttact tagttaagga
     1021 aaataaatat tttaaatccc ttgaatttag ttgattattt attctttgtc tatagtatta
     1081 aagagattgt ttagttttta attatgaata tatgctatta actggatatt gtaatttagt
     1141 aaaattttat tatacaactg atatataata aactaaaatg ataatattaa aaccttttcc
     1201 acatcattta ttttattaat tcataatatt acaataaaag aaagaaaatt ataaacttat
     1261 tattcaactg ctttatgtaa atatagttgg cattaaaaat ttataaaata atatatattt
     1321 taaattcaga aggtcacaaa atgatttcta catagctaat ggccttcaaa gtcttaatga
     1381 ataaagtcct ctcttgtaag aaacggttta ccgcacttat tacatatata ttgccgcata
     1441 gaatcattta atatagaaat cattattctc ttcggtggct aaattttttt ctctagaaag
     1501 tggatctttt actaaattga tgaatattgt tggttctgtt atttctaaag attactagac
     1561 tatttgttta ctacaattta catgttcctt gaatgaacgt aatggaaatc tattctttta
     1621 taatgaaatt aataaaactt ttttggttat ttaatttgta attggtatat ctgataattt
     1681 ctttttgtta aagagaatta tataactatt gttaagatag ttattagtat tggctacgat
     1741 aatttttatt tttcctatct attaaagatg tcatttgtta tgattaataa gctaattttc
     1801 ttatatttat catgaaacaa tagtactttt attttgtacg gttaggagga atattaaata
     1861 tctttatcca tgattctaac ttagtaaatt aatgagatga aagatacaat tcataagtac
     1921 gctgctattc ttttctttga atttttactc atttttgata tattcagtaa taaatcgaga
     1981 ccaaagtatg aattaaagaa ttcaatttcg atatttattt aaataagcag ctgtttaaat
     2041 gtcatctttt gtttgaacca attccgctag acctcctccg actgtttcat ttaattctcc
     2101 tcgtctttct aatgacttat aacatttcaa gcataaatat tttgaattgt agtgatttat
     2161 cacatgattt tttgtatatt taatacatat gggacacact actttattat acattttcac
     2221 tttttgaaaa cctcttgtca ttcttccaat taagccaatc ttgaaaaaat taggaaattt
     2281 cataaacaat cattaaaata aaaattttac cgtacttatg gcaaacaaga catgcaattt
     2341 gtggaatata tcggagatat tacagtttat acattctttc tttatcatat agcattgtag
     2401 atttcttttc ttatattagt tataccaatt ttttttcaaa ccttcttgtc atttttattc
     2461 aacattaatt tcataaagca gattattatc gacgttataa tggtttaata taatcaattg
     2521 gattatacta atatggtatc taagtgtatt ttataaatat tgatagtagt caatcactct
     2581 tggtactcat atcatagtta agagtcaata taatacaatt gaaaccatta catagtgatc
     2641 tttagtgatg ttattgttga tgtagtgtca tttattttct aatgctaacg aagggagtgg
     2701 taatcgaaaa aagaaaccca gttgtgattg cttttctatt gatgggattt gttgtatcat
     2761 tgagcgttga attgaatctg ctccacctgg aactagagct ggcttttgat ctgatgctgg
     2821 aggattacta ggtgttattg taaattcgtc ccttcttgtt ccttcatttg atatcatctt
     2881 acagctcatc atatcgttat taacacttca gtgcagagct ccattcaaac ggacatattg
     2941 ttttggtgga gaacctgatt ctcctgaatc ttggtgtgga ttagctcctc catttccaac
     3001 agtcacataa acaaaatctg tccctggaac tacattcatt ctagcgtaag agtggttatg
     3061 ccctctcaat atcattctga ttttatcata atttttaaac acatcaaaat atcttatgtc
     3121 agcatctggt ttctatgggt ttcctgaagt tggggttaat actgtcttgt gaccaaaagg
     3181 aataatgtgg tctagagttg ggtctgcctg tgtctgtgct aataatggat aaacagttga
     3241 tgatcattgt tttcggtatc aaaagcaaca aaaggaacat tacttaactt gtaaataaat
     3301 tcccatttac ctccattgat tggccataaa ttaaggaaat catcatttgg atcatcatga
     3361 ttaccaagag ctctaataac attgaaattg ttaagagcat ccatgttttt agaccaccac
     3421 tgtgcagcaa ttccattata tgaaaaatca ccaagtatca aaacaacctc tggattgttt
     3481 gctgccatgt tttcatcagt gttagtatta tctctaaagt caccatttcc tgtaaagttg
     3541 aatggagtgc taggaagaag gttgcctgaa cctcctcctg taccaccctc agctcctcct
     3601 ccttcagttt gtgggggcga taatggtgct ggtggttgtg ctgttgccga ttggtctgct
     3661 cctccaatgg gttgtgagga tggtattgaa agaggttttg ttgaatcata tatagtagtt
     3721 aagcaagttt taatctcctt atctttgatc tgaccttgac aatgattttg ttgaacagtt
     3781 tcataacctt gaatattatt tactgaaaca gaatattgca tcaatttgga cttgcggtat
     3841 taagacgtgg gaaaagaggg tggaattgca ggggttccca gcagatttat tataaaatca
     3901 ggtgtggttt gctgcctcca ttatcgttta tcgtattcgt tgctattatg aaaactgatt
     3961 tgagactgtg ctagtgatga tagttttagc tctaatttag aaacttcgaa tcatcttgca
     4021 gggcaaatat aataaccagt aatgtagtaa caaacaaaaa taaaattagg atatatagat
     4081 agctatcata tctttaaatc atcttttaaa atagatctac tgtatattag tagtcagtta
     4141 caaaagtaaa taagatgatt tttaaattct cattgttctt atttattaat aattatccta
     4201 taaaactatt taagttctta atttcataag aataaactta ataaaatata tatctaataa
     4261 aataagtgaa taggaggtaa agaattattt agtttgcatc ttttttctaa atagtattta
     4321 caataagaat aaatacaacg gtttcacaaa aattaaatta gaagctccct tcaaattaag
     4381 tattattaat acaaataata ttctcttctt aagatccttt ggaagttttt caaaatcaaa
     4441 agtaaatttt aaaagattat tttgttcatt taagattaat cataatactt taactttagg
     4501 ttaaggatat ggaaatgctt attaatttta gagatgattt ggtttatttt gtttttaatt
     4561 aattgtctct gttatgctag tttttaacgt tatcattcaa ttcgaaatta aaatttctaa
     4621 tatgaaatga aattccttct tcatataacc ccttacatat actctttatt atcccttctt
     4681 caattaattt aagtctattc ttgctttcaa taagatcagt aggaatatca atgtccattt
     4741 ctacttttaa ctttagaagt ttagtagata ccatgcattt aataaaatat gactgcgtat
     4801 ttagtttgcg tttttggttt ataaagtatt attaaaaatc ttaataaaaa aataacctga
     4861 tgtaatttgt tatagaaatt acagcacatt aaccaatttc attttctaat tttattaaaa
     4921 aatttatatg gaaatagaat tgaacaattc ttgggttaca acaggttatt agatcattgc
     4981 tgctctaatc taacctttgc tattttgaga tatattaata attcaaaatt ataactagtc
     5041 catttcttta ttattaaaag ttagaaccat aatattagag caagatctaa aattttagat
     5101 tcagaatgat ctgtattgta ttggctattg atttctaatt aatcataatc ttggtttcta
     5161 aagttatata gtagttattt attattataa agaattataa tgacgaataa taagaaatat
     5221 tgattattgt tttaaaaaat aaaataattt gagtttataa gatttataat tataatatac
     5281 tatacctctt tatactacta atccaatgga ttacaaaata ttattgtgag gtgaattggt
     5341 taatttatag tatttatata agtcatatgc ttttttgctc attatgggaa tgtttgtcta
     5401 gcaataacta tgaaaagctt ttagaataaa tccattatat caaatactct taggataaga
     5461 tgaagattat ttagaatgca aaatctaact ctaatccttg aagcgaatga actttagcaa
     5521 taataagaac aaaaaataaa gatgtatact ctttatcttt tgatatatag atggcttact
     5581 agagttgcta ccaaagtaaa agacccaatt aatgcagcaa taaatacggt atctgctact
     5641 gatgctagtg gttcacccat gactgctacg aatatcgaat aaacaagaat aataaatcca
     5701 attaaagcga ataatcctaa cattattg
//
LOCUS       DASVEV010000230         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1273978, whole genome shotgun sequence.
ACCESSION   DASVEV010000230 DASVEV010000000
VERSION     DASVEV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273978"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..956
                     /locus_tag="VF222_10215"
     CDS             291..956
                     /locus_tag="VF222_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142837.1"
                     /translation="MLRSLELRWFFKGVLPIDIYQWFTDLLPGNENYEEKPRVDYYLT
                     TQSDDLGVKFSRQQLQVKIRKDNIDFSLNDNNIQGSLEYWIRYDWNDLHINENTKIDN
                     LYDRFSSMKIDKKRLIRKYKILDNQLIHVPLSNTTDIQCSIEITKINVKEQVWSTLGF
                     DWFLNNKIPEFTNIVDYITNNKTFNKTIEELFNQYPDFKITKKPNSFGYPYFLSKMLK
                     EEK"
     gene            1269..1670
                     /locus_tag="VF222_10220"
     CDS             1269..1670
                     /locus_tag="VF222_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142838.1"
                     /translation="MAKQKNINKKKSTGNSNAPGIYTSLSEDRVKYDKQIDIPNPKLQ
                     PNKTFEYTLFNTPSNGNANPPSAMNNVFFPELEKLSRKLNKAFEKKGAKYLLKKQKEL
                     NDNTIQISIALLDESVYLTYTKPTLEKTSQK"
     gene            1736..2002
                     /locus_tag="VF222_10225"
     CDS             1736..2002
                     /locus_tag="VF222_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142839.1"
                     /translation="MNLDPNFVWNKSSMKKKVIPYSAIDIDAIWGSSNTKGLSELQTL
                     WYSFLDDFTKNNITRLNIGEMITVHSAFKHPIKITFPSPSYKRI"
     gene            2427..>3150
                     /locus_tag="VF222_10230"
     CDS             2427..>3150
                     /locus_tag="VF222_10230"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7142840.1"
                     /translation="MKNFSSKGIKFSGNSIKCCIGFIDLVDSTKNTITMDNLESIRRY
                     YSTFINSVSENVRSYSGKVLKNIGDCLLFYFPKTSYNKNIDAFKEAIDCSFNILDERY
                     NINKELTRQYLPPFNYRISIDYGLVDLASMGEYSQIDLFGSTINLCSKINSSLSIPNE
                     IIIGDNIYRVLRSFPSIAENFKFSNNSEYKITDIVSYPTYNIKRENIFTLTEKQLGSS
                     IKNLETKNENGFIFHDENSNKRV"
BASE COUNT         1275 a          399 c          392 g         1084 t
ORIGIN      
        1 atacaaaagt aaaagataat aagaacaaaa aagaattata caaattaata attgattatt
       61 gattaaatta ttttttttac cttattattc taatttttca aggaaatata tagacaataa
      121 tagttcatat tttattttac attctctttt actatggtaa agttttgtat aaatcagtat
      181 tcggaaataa aataaaagga taatatccca ctagtgaaga taaagatatc atagtatacc
      241 acaaatataa ataataaatg taatttatat aatagtaatt atttactatt atgttaagat
      301 cattagaatt aagatggttc ttcaaaggtg tattacctat agatatatac caatggttta
      361 cagatctttt accaggcaat gaaaattatg aagagaaacc tagagtagat tactatttaa
      421 ctacccaatc agacgattta ggagtaaaat tcagtagaca acaattacaa gtaaaaatta
      481 gaaaagataa cattgatttt tctttgaatg ataacaatat tcaaggatca ttagaatatt
      541 ggattagata tgattggaat gatttacata ttaacgaaaa tacaaaaata gacaacctat
      601 acgatagatt ctcttctatg aaaatagaca aaaaaaggct aataagaaaa tacaagattt
      661 tagataatca acttattcac gttccattat ctaacacaac cgacatacaa tgctctatag
      721 aaattacaaa aataaatgtg aaagagcaag tctggtctac attgggattt gattggtttc
      781 taaacaacaa aatacctgaa tttactaata ttgttgatta tataactaat aacaaaactt
      841 ttaataaaac aatagaagag ttattcaatc agtatcccga ctttaaaata actaaaaaac
      901 ctaattcatt tggttatcct tattttttat caaagatgtt aaaagaagag aaataactat
      961 atcaaaggag gtgataatgg tttcataact tatccggaga aagtaaatca tcaagttatg
     1021 aaagaaaaaa ttactcccca gatgtatgta agacagaggt caatgatttg taattattat
     1081 taagattcta tgattaatca gcatttacaa tatatattat aataatctat ataccagtag
     1141 gtatattcta tagattcaac aaaatctttt gataaacttg ctattttgaa tacaattaaa
     1201 attaaaatat ttatgattta ttctcaattt aaaaatattt ttatatttct gttaagagga
     1261 tataattatt ggcaaaacaa aaaaatataa acaaaaagaa atctactggt aattctaatg
     1321 ccccgggtat atatacttca ttatcagaag atagagtcaa atatgacaaa cagatagaca
     1381 ttccaaatcc aaaacttcaa cctaataaaa catttgaata tacacttttt aataccccat
     1441 caaacggaaa cgctaaccca ccttcagcaa tgaacaatgt cttctttcca gagttagaaa
     1501 aattatcaag aaaattaaat aaagcatttg aaaaaaaggg agcaaaatat ctcttaaaaa
     1561 aacaaaagga attgaatgat aatacaattc aaatctcaat agcattgttg gatgaaagtg
     1621 tatatcttac atatacaaaa cctactttag agaaaacttc acaaaagtaa atcaagattc
     1681 aaaattctac ttactactat tcttctcatt ttttaaagtc aattaataac gggtcatgaa
     1741 tttagatccc aattttgtct ggaacaaatc atctatgaag aaaaaagtaa taccatattc
     1801 tgctattgat atagatgcaa tatggggatc cagtaataca aagggactat cagaattaca
     1861 aaccttatgg tattcatttt tagatgattt cacaaaaaat aatattacaa gattaaatat
     1921 tggagaaatg attacggttc attctgcttt taaacatcca attaaaatta cttttccatc
     1981 tccatcttat aaacgaatat aaaaaccaca ttacaattta gaaagctttt ttagatattt
     2041 aactttatta aagtagtcta attctccaaa attttcggag ttctgtattc attctttttt
     2101 atgaagcaaa tttattttgc tgtaaataaa gatataagaa aaatagtata accatttgtt
     2161 tttttagcat tcatatatta ctattgttat ttaacatatt tggtacttgg ctcaacaatg
     2221 ttaacttctc acgtttttca ttctcctcta tatagaacag ttagcgaaaa cttttcagat
     2281 atcaaatctt agattagctg aacaaatatg tcattaatta ttgctgttac gggtcgtatc
     2341 atagtcatag ataataaata ttacaaggtt aagatcttta tgaatataag tacttcatgt
     2401 gattacgatt ttggaggcta caataaatga aaaatttttc atctaaaggt attaaatttt
     2461 ctggcaattc gataaaatgt tgtataggtt ttattgattt ggtagattca actaaaaata
     2521 ccattacaat ggataattta gaatctatta gaagatacta ttctacattt ataaattcag
     2581 tatcggaaaa tgttagaagt tatagcggaa aggttcttaa aaatattggt gattgtttat
     2641 tattttactt tcctaaaact tcatataata aaaatattga tgcctttaaa gaagctattg
     2701 attgtagttt taatattctc gacgagcgat ataatattaa taaagaatta acgaggcagt
     2761 atctaccccc attcaactat agaattagta tagattatgg tttggtagat ttagcttcaa
     2821 tgggagaata tagtcaaata gatttatttg gatcaactat aaatctttgt tcaaaaataa
     2881 attcttcttt atctattcct aatgaaatta ttattggaga taatatttat agagttctga
     2941 gatcgtttcc ctctatagcc gaaaatttta aattttccaa taatagtgaa tataaaataa
     3001 ccgacattgt cagttatcct acatataata ttaaaagaga aaatattttt acccttactg
     3061 agaaacaatt aggttcatct attaagaacc ttgaaactaa aaatgaaaat ggtttcattt
     3121 ttcatgatga aaatagtaac aaaagagtaa
//
LOCUS       DASVEV010000231         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_305899, whole genome shotgun sequence.
ACCESSION   DASVEV010000231 DASVEV010000000
VERSION     DASVEV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305899"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF222_10235"
     CDS             complement(<1..659)
                     /locus_tag="VF222_10235"
                     /inference="COORDINATES: protein motif:HMM:NF020078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HEX7142841.1"
                     /translation="MNSDTNDTNILHIGLDDTDSTFGRCTTHLAFKVTSYLSKKNVKF
                     LDYPLLIRLNPNIPWKTRGNGAVCLRIVTKAKNNENIIDFIKNELEKNSEIDKGANPG
                     LVCLQSKEIPDEIKEFHRLAMFDILSKQKATKLAQKYDLKYFSFGNGQGLVGAIAAIG
                     CLLNSDHTFEILAYRKIKNCGTLRIIDPNNVIRLHDTTFPSTYNSYDYIHKRVLILPH
                     GP"
     gene            797..1138
                     /gene="hisI"
                     /locus_tag="VF222_10240"
     CDS             797..1138
                     /gene="hisI"
                     /locus_tag="VF222_10240"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7142842.1"
                     /translation="MQFKVLEDLDFTKRGGLLPVIVQDQMTKDILMLAYVNREALENT
                     IKTGNAWFWSTSHKKLWMKGEESGNIQPIEKIMVDCDADALLYIVNSNKPVCHTGNIS
                     CFHNVLKDKNY"
     gene            1306..2529
                     /gene="thrC"
                     /locus_tag="VF222_10245"
     CDS             1306..2529
                     /gene="thrC"
                     /locus_tag="VF222_10245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7142843.1"
                     /translation="MGIIQSLICRECKKEYKPQFRYICEDCFGPLDVKYIYPNNIKRE
                     LFESRKEKTYWRYFELLPIRDKNNIVSINSGFTPLQKSTNLSTDLRLKSLYIKNDSVN
                     PTFSFKDRPAGVAVSRAKEINLKAVGCASTGNLASATAAHAAVARLPCYIFAPADLEP
                     VKISQALSYGAEFVAVDGTYDDANRVASVIGDSKGIGIVNINMRPYYVEGSKTLAYEV
                     AEQLNWQVPDYLVIPVGSGAMLNAICKGFEELCEIGLVNDVSNLKIIAAQPHGCSPIV
                     DAFKSDSNEIFPVEKPNTIAKSLAIGDPGDGIYVLKRLKQYHGIAETATNQEIIEGIL
                     KLSKTEGIFTEPAGGVSVAVLQKLVESGKIDRDANIVCYVTGNGLKSTEAIINMLPKP
                     YTVKPDVQMVSTIIR"
     gene            2579..>3288
                     /locus_tag="VF222_10250"
     CDS             2579..>3288
                     /locus_tag="VF222_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX7142844.1"
                     /translation="MAKIEFIIPSVLNKGSGEKKILLDVQDLHDAFNKASDNLGDDFK
                     RRVLDLNGKPRALINIYINGKNMRFNKDGMTTSLKDGDSIYILPAVAGGANETKFERI
                     ELSSQDIQRYSRQIMLEEIGFLGMEKLRNSKVCVVGVGGIGNPVVTQLTAMGVGKIRI
                     VDRDVVEISNLHRQHLYTEQDIGKVKVEAAIEHLKKRNTNVEIEALPISITSYTAENI
                     VKGFDVVIDALDSIDARYA"
BASE COUNT         1179 a          437 c          571 g         1101 t
ORIGIN      
        1 ggtccatgtg gtagtataag aactcgttta tgtatgtaat cataactgtt atatgtagag
       61 gggaatgttg tgtcgtggag ccgaataaca ttgtttggat caattattct aagagttccg
      121 caatttttaa tttttctata tgctaatatt tcaaatgtat gatctgaatt taataaacat
      181 ccaatagcag ctatagctcc aactagtcct tgaccatttc caaaactaaa atattttaaa
      241 tcatattttt gtgctagttt tgttgctttt tgtttactaa gtatatcaaa cattgcaagt
      301 ctatggaatt ctttaatttc atcaggaatc tctttgcttt gcaaacagac caatcctgga
      361 ttagcgcctt tatcgatttc agaatttttt tctagctcat ttttaataaa atcaattata
      421 ttttcattat ttttagcttt agtcactatt ctcaaacaaa cagcaccatt tccacgagtt
      481 ttccagggaa tgttgggatt taatcttatt aacaaaggat aatctagaaa tttcacattt
      541 tttttgctaa gataagatgt aactttgaaa gctaaatgag tagtacatct tccaaatgtt
      601 gaatcggtat catctaaccc tatatgcaga atattagtgt catttgtatc tgagttcatc
      661 aacgtttcta aataagatta aatcattaaa taaatttaat ttcttcttga aatgaatgag
      721 gataagaaaa attaaaaata atattaactg atattttata attacttttt tatattgaat
      781 attaaaataa aagattatgc aatttaaagt gttggaagat cttgatttta caaaacgtgg
      841 gggattatta ccagtaatag ttcaagatca aatgactaaa gatattctta tgcttgcata
      901 tgtcaataga gaggctcttg aaaatactat caaaactggc aatgcatggt tttggagtac
      961 ttcccataaa aaattatgga tgaaaggtga agaatcagga aatatccaac ctatagagaa
     1021 aatcatggtt gattgtgatg ctgatgcatt attgtatatt gtaaattcca ataaacctgt
     1081 atgtcatact ggaaatattt cttgttttca taatgttcta aaagataaaa attattgaaa
     1141 agtatattat aattaataat atttttatat aattagtata agaaagaata atatcgtatt
     1201 atttcgtttc aacattctat tttatttata atatgaaatt atagtgacac taaatttatg
     1261 tttagagaac cttatttata atgtatttta actgcattat tataaatggg aataatacag
     1321 tctttgatat gcagggaatg caaaaaagaa tataaacctc aatttagata catttgcgaa
     1381 gattgttttg gacctctgga cgttaaatat atttatccta ataatataaa aagagaatta
     1441 tttgaatctc gaaaagaaaa aacctattgg aggtattttg aactcttacc aataagagat
     1501 aaaaataata tagttagtat taattcaggt tttactcctt tacaaaaatc tactaatctt
     1561 agtacagatt tgcgattaaa atctttatac attaaaaatg attctgtcaa tccaaccttc
     1621 tctttcaaag ataggcctgc aggtgttgct gtatctagag caaaagaaat taatctaaaa
     1681 gccgtaggct gcgcttcaac aggaaatctt gcatctgcta ctgctgcgca tgctgcagtg
     1741 gctagacttc cttgctatat atttgcgcct gctgatttag aacctgtaaa aatctctcag
     1801 gcattatctt atggtgcaga atttgttgct gtagatggaa cttatgacga tgccaatagg
     1861 gtagcatcgg taattggaga ctcaaaagga ataggtatag tgaatattaa tatgagacca
     1921 tattatgttg aaggatcaaa aacccttgct tatgaagtag cagagcaact taattggcaa
     1981 gtaccagact atctagtaat tcctgttggt agtggtgcaa tgttaaatgc aatttgtaaa
     2041 ggctttgagg aattatgtga aataggattg gttaatgatg tttctaattt aaagattatt
     2101 gctgcacaac ctcatggatg ttcaccaata gtagacgcct ttaaatcaga tagcaatgaa
     2161 atatttcctg tagaaaaacc gaatactatt gcaaaaagcc ttgctatagg ggatccagga
     2221 gacggaattt atgttttaaa aaggctaaag caatatcatg gtattgctga aacagctaca
     2281 aatcaagaaa taattgaggg gatacttaaa ttgtcgaaga ctgaaggtat tttcacagaa
     2341 cccgccggtg gcgtatctgt agctgtcctt caaaaattag ttgaaagtgg taagatagac
     2401 cgggatgcaa atatagtttg ttatgttact ggtaatggtc tcaaatctac tgaagctata
     2461 ataaacatgc tgccaaaacc ctatactgtt aaacccgatg tccaaatggt ttctacaata
     2521 attagataat aaaaataata atatgaataa tatatgtgaa ataaaagtga agatataaat
     2581 ggcaaaaatt gaatttataa ttccatctgt tttaaataaa ggttctggag aaaaaaaaat
     2641 attattagac gtacaggatt tacatgatgc ctttaataag gcatcggata atttaggtga
     2701 tgattttaaa cgtagagtat tagatcttaa cggcaaacct cgagctctca taaatatcta
     2761 tataaatggg aaaaatatgc gctttaacaa agatggaatg acaacatcac ttaaagatgg
     2821 tgattcaatt tatatactac ctgcagtagc tggaggagca aatgaaacaa aatttgagcg
     2881 tatagaactt agtagtcaag atatccaacg ttattctaga caaataatgt tagaagaaat
     2941 aggatttctt ggaatggaaa aacttagaaa ctccaaagtt tgtgttgttg gagtcggtgg
     3001 aattggaaat ccggttgtaa ctcaattaac tgctatgggt gttggaaaga tccgcattgt
     3061 agatagagat gtagttgaaa tatctaattt gcatcgacaa catttgtata ctgaacaaga
     3121 tattggaaaa gtcaaagttg aagcagcaat agaacatttg aaaaaaagga atactaatgt
     3181 agagattgaa gctcttccta tttcaattac tagttacact gcagaaaata tagttaaagg
     3241 atttgatgtt gttatagatg cacttgatag tattgatgca agatatgc
//
LOCUS       DASVEV010000232        11335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1665347, whole genome shotgun sequence.
ACCESSION   DASVEV010000232 DASVEV010000000
VERSION     DASVEV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665347"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VF222_10255"
     CDS             complement(<1..1112)
                     /locus_tag="VF222_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX7142845.1"
                     /translation="MITNSINSIISQPTQAIEKVLSGEELTYNDGLQLFEENLFLLGS
                     VADQIRRKSKGNTVTFVSSYYLNYTNVCAASCSLCAFYRKGHEDDAYTLTNEQIIERA
                     RIAIVQMGASELHIVGGFHPKLGLEYYENMIKNIKKEFPFVKVKALTPAEIFFISKLT
                     KNSVKEILLRLKDSGLDALPGGGAEIFSSDIRNIIVRGKCSGEEWLDTAKQAHEIGLK
                     TNSTMLFGHIEKPEHIIDHIIKIRELNKKTHGFNTFIPLKFSLENTELEKKKLIVRES
                     PSTYDLRIIAIARLLLKDVLDNISVYWVSLGKKIAQVALTYGGNDLVGTAFSEEIFRA
                     AGKASGISIQELVNLIKEIKREPAQRDTFFKVIEKY"
     gene            1218..2339
                     /gene="mqnC"
                     /locus_tag="VF222_10260"
     CDS             1218..2339
                     /gene="mqnC"
                     /locus_tag="VF222_10260"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX7142846.1"
                     /translation="MQNSLNYIVSKSEIGYILNNVLNGERLNLNDCKELVKSDDVYTI
                     GLIANTLRKILFGNTATFINNIILNYTNVCVTYCKFCAFYRPPGHEESYTHNIKDIMK
                     RIALSKEMFGITQILFQGGHNPNLSIEYYEEIFNEIKKFYPSIAIHGLSASEIDMISK
                     VERSSIKEVLSRLKDAGLESLPGAGAEILNDKVKSVISPLKIKSNQWLNIMRDAHDLG
                     IKSSATMMYGTVETDDDIAQHIMKISELQQKTNGFIAFIPWGFEPNNTQMQTEGIAKY
                     SSGGLRLLKMIAISRIMFYGLISHIQSSWLTNGIGMAQLALHFGADDFGGTLIGEEVV
                     SATGARSTELTSIKIIESIKQAGYYAAERDNFYNVIKNF"
     gene            2468..3865
                     /locus_tag="VF222_10265"
     CDS             2468..3865
                     /locus_tag="VF222_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX7142847.1"
                     /translation="MPVGIDDMALYVPKLYIDSKDFAIARGIDPHKLEIGIGIYKMAM
                     VDTNQDPACMAANACLKLMQKNDLKPQDIGRMYVATESALDESKAMNSFVIGMLEQIY
                     GDGSFEHAGGIECKFACVSGSYALYDNANWIRAEENNDRAAIVIVSDIAKYDLGSAGE
                     YTQGAGAIAMLVKENPRLVVFDPKVTSTIIKNEYDFYRPFGRETPLVNGNYSNYLYLI
                     QIKKAFDSYREKAIKTKLIQLQEDESITDKIDYISVHLPYRKMGEKALAYLLRHEWRC
                     LPRWKQIIEEVGMDEPTPKDPRGTIESILADKDFMKEDDKFRKAFMRTSFFKQVFEQK
                     MASSLEASAIIGNLYTASMYMGLRSLLEFEYKKNIDLENKRIGFGSYGSGSSAMVFSG
                     VIQSTYKEIVKDMNLEREIGDRSKLDMQDYIRLHSNEGKMNEPISNSNNEFVLVKIGG
                     NTADKAGLREYIFSN"
     gene            complement(3944..4444)
                     /locus_tag="VF222_10270"
     CDS             complement(3944..4444)
                     /locus_tag="VF222_10270"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7142848.1"
                     /translation="MSILDGFIIWIHLLSASIWVGGSIFIGIILAPMLKHIGNSMEER
                     IIIMIKIGRRFNNIAFPSFIILISTGLYNSRFFFEYPYNFIETDYGILLLIKIVLVII
                     TFAIYIIHIKSFGKETEARIIDNRGSEYISIIRSKIINYGRLTVILSIIILLLAAFLD
                     AGIKFS"
     gene            4561..5076
                     /locus_tag="VF222_10275"
     CDS             4561..5076
                     /locus_tag="VF222_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142849.1"
                     /translation="MFLSKRRIVLFGVIGALVITIIFLPRIQYFINAPDVNVIDFNLA
                     AVNISNLSNNTNKIELSLLFDVYNPSDKSATTSKVEYDLFGDNKLLGHGVLSYEDIPL
                     NGRPQFSPGQTATLKSAIILQPSDMNMDVIEKLISNSSDSINSIRWNVNGSSQIESAF
                     VILEKDFSDVL"
     gene            complement(5115..5558)
                     /locus_tag="VF222_10280"
     CDS             complement(5115..5558)
                     /locus_tag="VF222_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7142850.1"
                     /translation="MSSNVNIPKLPAKNKSELTEQVFQAIVDSGETGIFQTELCKSFS
                     IDSRDGSRLVGNLEKQSLISRERMLFKGRWTYKLIVKKIPQLKEADRRPIEIASVEGA
                     PCFSCIYQHLCSTEDETSQYSPAKCNWIEQWVILDTKKRGLSNQL"
     gene            5819..6385
                     /locus_tag="VF222_10285"
     CDS             5819..6385
                     /locus_tag="VF222_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142851.1"
                     /translation="MTKILGKNITIRSIMIFSTTIVASGLLLFSFNVDVIMGIPADLV
                     KGDYLFGRTIGIVEDENGKPVWIISGIWKTNLSNQTQEATSNSTVFDASFEMIKTDGT
                     SKHTHTMTNFVLADTLNQNKNSTIFNGTETISMPQGPVTEVPISIQVMNNSLGIINID
                     PNKIDNHFGDDPLYGIPLEESEQYKKKS"
     gene            6476..7459
                     /locus_tag="VF222_10290"
     CDS             6476..7459
                     /locus_tag="VF222_10290"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7142852.1"
                     /translation="MNFKNTVFFSGMAVLLSFLILNGSSVTYQSSFATATTTEYEHKM
                     KWGSKGVATGQFNQASGIVADSENNIYVADFTGRSNMIQKFTNNGSFILGFGILGSGP
                     QFFTNPTGLTADSDNNIYIADFGNPTYAIKKYSSDGKFIDSFGSFGLGDGEFITPGGL
                     GVDKENNIYATDFGENNNVQKFDKNGNFIMKFGTYGSGDGKFLNPTDVAIDSLGNIFV
                     VDSFNERVQKFDKNGNFIMKFGKSGTGNGEFNTPIELAIDSSDNIFVVDNGNNRVQVF
                     NRDGGYITQFGNQGPISDILQGPVGIAIDSNGNVYIADGIDSDIHIFSQVN"
     gene            7923..8072
                     /locus_tag="VF222_10295"
     CDS             7923..8072
                     /locus_tag="VF222_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142853.1"
                     /translation="MAKQGMTIVSRFFLQNAEPNKASGLLSMLPSSITDIFSNDEKKE
                     FTTSQ"
     gene            complement(8282..8668)
                     /locus_tag="VF222_10300"
     CDS             complement(8282..8668)
                     /locus_tag="VF222_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142854.1"
                     /translation="MAKKRVLIIDDDKDINNLFKIYLEKNGRYQIDAYTDPVNALYYF
                     KKGLYDLVLLDLKMPQIDGITMYKQLKNIDNNTSICLITADIAHLEQLKEKIPNIEKY
                     VIYKPILLKNLKDKIDSLLFEKNMFC"
     gene            complement(8818..9234)
                     /locus_tag="VF222_10305"
     CDS             complement(8818..9234)
                     /locus_tag="VF222_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142855.1"
                     /translation="MELSNHTDTYILLVDDEKDVLDLFSEYLTSSGFNTISFDNPVKA
                     LEYFYKNTNNCSVIITDYRMPQMSGLDFINKVREKDTDCKIKTIIISAYIKDNIPYDK
                     SYLMKIDKILEKPVYLDKLKTEIQQLLTIDIKKKIA"
     gene            10491..>11335
                     /locus_tag="VF222_10310"
     CDS             10491..>11335
                     /locus_tag="VF222_10310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7142856.1"
                     /translation="MVNKKKQWRGKAILHVDINSFYSSCEEIKDPSLIGKPHAVIMTP
                     QNNNNITKGAVTTCSYEAKKLGIKSAMPLYKALELCPNLILHAVDKKFYEKISNEVME
                     ILEDYADIFEQASIDEAYLECTNKISNTHLTVEEYAQEIKKTIKEKCSGLLTSIGIAP
                     TKSVAKIASDYQKPDGLTKVNFNELKNFLSPLDVETISGIGIKTQKILKEQMNIKTIG
                     QLSNTDVQLLIERFGKKIGTWMWQVANGLDNEPVLPRGNHISLSNESTLEFVTEDKEM
                     IKRSL"
BASE COUNT         4135 a         1499 c         1660 g         4041 t
ORIGIN      
        1 tagtattttt ctattacttt aaaaaatgta tctcgttgag caggttctct ttttatttct
       61 ttgatcaaat ttacaagttc ctgtattgat ataccactag cttttcccgc agcacgaaat
      121 atttcttctg aaaaagctgt accaactaaa tcattacctc catatgtcag tgctacttgt
      181 gctatttttt tacctagaga aacccaataa actgatatat tatctaaaac atcttttaat
      241 aacaatctag caatagctat tattcttaag tcataagtag agggactttc tctaacaatc
      301 aattttttct tctctagttc agtattctct agactaaact taagtggtat aaaagtattg
      361 aagccatgag tttttttatt aagctctcta atttttataa tatgatcaat tatatgctct
      421 ggcttctcaa tatgtccaaa aagcatagta gaattagttt tcaaaccaat ttcatgagct
      481 tgtttagctg tatcaagcca ttcctctcct gagcattttc ctcttacaat aatatttctt
      541 atatctgagc taaagatttc tgcgcctcct ccaggaagag catccaaacc agaatctttt
      601 aatcttaata aaatctcttt aactgaattt ttggttaatt tagatatgaa aaaaatttct
      661 gcaggagtaa gagctttaac ttttacaaaa ggaaattcct tttttatatt ctttatcatg
      721 ttttcataat attccaagcc aagttttgga tgaaatcctc caactatgtg taattctgaa
      781 gcacccattt gtactatagc tattctagct ctttcaataa tctgttcatt agttaatgta
      841 taagcatcgt cttcatgccc ttttctataa aaagcacata aagaacaact tgctgcacaa
      901 acattagtat aatttaaata atatgatgaa acaaaagtca ctgtatttcc ttttgatttt
      961 cttctgattt gatcagcaac agaacctaat aagaatagat tttcttcaaa taattgtaaa
     1021 ccatcattat acgttaattc ctctccagat aatacttttt caatagcttg tgtaggttgt
     1081 gatataatgc tattaatact attagtaatc aatttagtta aataaattaa tttattaatt
     1141 gatatatatt cttctatatt acagaagata tattttcttg taaagaataa atagaataat
     1201 caagctataa ttttcaattg caaaatagtt taaattatat tgtttcaaaa tcggaaattg
     1261 gatatatttt aaataatgta ttaaacggag agagactaaa tcttaatgat tgtaaggaat
     1321 tagtaaaatc tgatgacgta tatacaattg gccttattgc taatactctt agaaaaattc
     1381 tttttggtaa tacagccact tttataaata acattatttt gaattatact aatgtttgtg
     1441 taacctactg taaattttgt gctttttatc gacctcctgg ccatgaagaa tcatatacac
     1501 acaacatcaa agacataatg aagagaatcg ccttgtcaaa agaaatgttt ggaattactc
     1561 aaattctttt tcaaggggga cataacccaa atttatctat tgaatattat gaagaaatat
     1621 tcaatgaaat aaaaaaattt tatccgtcaa ttgccattca tggtctttct gcttctgaaa
     1681 ttgatatgat atctaaagtt gaaagaagta gtatcaagga agtcctatct agactaaaag
     1741 atgcaggatt ggaatcatta ccaggagcag gagcagaaat tcttaatgat aaagtaaaat
     1801 cagtaataag cccccttaag ataaaaagta atcaatggtt aaatataatg agggatgcac
     1861 atgatcttgg aatcaagtct tccgcaacaa tgatgtatgg aactgtagaa accgatgatg
     1921 atatagcaca acatataatg aaaatatctg aattacaaca aaaaactaat ggatttatag
     1981 cttttattcc ttggggattt gaacctaata acactcaaat gcaaacagaa ggaattgcta
     2041 aatattcttc tggaggatta aggctactaa aaatgattgc aatatcaaga attatgtttt
     2101 atgggctaat ctctcacatt caatcatcat ggcttacaaa tggaattgga atggcacaat
     2161 tggctcttca ttttggtgct gatgattttg gtggaacatt gatcggtgaa gaggtagtta
     2221 gtgcaacagg agcacgatca actgaactta cttctattaa gattattgaa tctattaaac
     2281 aagctggata ttatgctgca gaacgagata atttttataa tgtaataaaa aacttctaat
     2341 gcataatcta agagataacc gattgatata atagatctta gctataaaat aacatagact
     2401 atttttagta aaaaaactta ataataatca caagtacgtt taaatgtctt ttagcaaatt
     2461 gaattttatg cctgttggta ttgatgatat ggcactttat gttccaaagt tatacatcga
     2521 ttcaaaggat tttgcaatag ctagaggtat agatcctcat aaactggaaa ttgggatagg
     2581 aatatataaa atggctatgg ttgatactaa tcaggatcct gcttgtatgg cagcaaatgc
     2641 ttgtttaaaa ttaatgcaaa aaaatgatct taaaccacag gatattggta gaatgtatgt
     2701 tgcaactgaa tcagcattag acgaatcaaa agctatgaat tcttttgtaa ttggaatgct
     2761 tgagcagatt tacggtgatg gatcttttga acatgctggc ggaatagaat gtaagtttgc
     2821 ttgtgtaagt ggttcctatg cattatatga caatgctaat tggatcagag ctgaagaaaa
     2881 taatgataga gctgctattg ttattgtaag tgatattgca aaatatgatt taggatcagc
     2941 tggagagtat acgcagggag ctggtgcaat agctatgtta gtaaaggaaa atcccagatt
     3001 agttgttttt gatcctaaag ttacttctac aataattaaa aatgagtatg atttttatag
     3061 gccttttgga cgtgaaactc ctttagttaa tggaaattat tctaattatt tgtatcttat
     3121 tcaaatcaaa aaagcctttg atagttatcg agaaaaagca attaaaacta aattaattca
     3181 attgcaagaa gatgaatcca taaccgataa aatcgattat attagtgtac atttacctta
     3241 tcgaaaaatg ggtgaaaaag cattagcata tctactacga cacgaatgga gatgtctacc
     3301 aagatggaaa caaataatag aagaagtagg aatggatgaa cctactccta aggatcctcg
     3361 tggaacaatt gaatccattt tagctgataa ggattttatg aaagaagatg acaagtttag
     3421 gaaagcgttt atgcgtactt ctttcttcaa acaagttttt gaacaaaaaa tggctagctc
     3481 tcttgaagct tcagctataa tagggaatct ttacaccgca tccatgtata tgggattaag
     3541 aagtttatta gagttcgaat ataagaaaaa tattgattta gaaaataaaa gaatcggatt
     3601 tggatcatat ggaagcggta gtagtgctat ggtctttagt ggtgttatac aatccaccta
     3661 taaggaaatt gttaaagata tgaatttaga aagagaaata ggcgatcgtt ctaaattgga
     3721 tatgcaggac tacatccgtc ttcattcaaa tgaaggaaaa atgaatgaac caatttcaaa
     3781 ttctaataat gaatttgttt tagtaaaaat aggtggtaat acagctgata aggctggatt
     3841 aagagaatat attttttcta actaaatttt tatttttata ggttaatgca aaattttttt
     3901 aaaattatat aatgatttag aataataatt tattggttag acatcaacta aattttatac
     3961 ctgcatcaag aaaagccgct agtaacaaga ttattataga gagaatcaca gttaatcttc
     4021 catagttaat aatttttgaa cggattatac taatatattc tgatcctcta ttatcaataa
     4081 ttcttgcttc tgtctctttt ccgaaacttt taatatggat aatatagatt gcaaaagtaa
     4141 taattactaa tacaattttt atcaacaaca aaattccata atctgtttca ataaaattat
     4201 acggatattc aaaaaaaaat cttgaattat acaatcctgt tgagatcaaa ataataaatg
     4261 aaggaaatgc aatattgtta aatcttctac caattttaat cataataata attctttctt
     4321 ccattgaatt tccaatatgc tttaacatgg gagcaagaat tattcctata aagattgatc
     4381 caccaaccca aatacttgca gataataagt gaatccatat aataaaacca tcaagtatac
     4441 tcatagttca acagtgttag tttaaaccat ctaatcactt taatgtatct ttatagtgct
     4501 atatgataat aaaaattaag aaacatttta attctttttt tgaaactgta tttataatat
     4561 atgttcctta gtaaaagaag aatagtactt tttggagtaa taggtgcttt agtgattact
     4621 attattttcc ttccacgaat ccaatatttt attaatgctc ctgatgtgaa tgtaattgat
     4681 tttaacctag cagctgttaa tattagtaat ctttcaaata atactaacaa aattgagcta
     4741 tccttacttt ttgatgttta taatccttct gacaaaagtg caacaacatc aaaagttgaa
     4801 tatgatcttt ttggagataa taaattatta ggtcatggtg ttttatctta tgaagatatt
     4861 ccattaaatg gtaggccaca gttttctcct gggcaaaccg ctactttaaa atctgctatt
     4921 attcttcaac catctgatat gaatatggat gtaattgaaa aattaataag caattcatca
     4981 gattcaataa atagtataag atggaatgtt aatggatcta gtcaaataga atctgcattt
     5041 gttattcttg aaaaagactt cagtgatgtt ctttaatttt tagataataa aataatggaa
     5101 tttttttgtt aaatctatag ctggtttgat agtcctcttt tttttgtatc aagaataacc
     5161 cattgctcaa tccagttaca ttttgcagga ctgtattggc ttgtctcatc ctctgtagaa
     5221 cataaatgtt gataaataca actaaaacat ggagctcctt ctacactagc aatctcaatg
     5281 ggtctcctat ctgcttcttt taattgaggt atttttttta ctattaattt ataagtccac
     5341 ctgcctttga ataacattct ttctctagaa ataagggatt gtttttcaag atttccaacc
     5401 aatcttgacc cgtctctgct gtctatagag aacgatttgc aaagttctgt ttgaaatatt
     5461 cctgtttctc cactatctac tatagcttga aacacttgtt ctgttaattc acttttattt
     5521 tttgcaggca acttgggtat atttacattt gaagacaaaa gataatactt tttagtttat
     5581 ttggtataaa aatctaatga atttgcttat gaaaaatcta agatatttaa tattatatag
     5641 ccagttaatt tgttagataa ctagaatagt cattaataat gaaagtttca aaaaatatta
     5701 ttaatttcac gatttagata cttctatatc attgaaataa atattattta ttgatgaaag
     5761 tgataatata gtaatagaag cgaaaaagta ataagaacaa tgtaaccaga ataatttgtt
     5821 gacaaaaatt ttaggcaaaa atatcacaat aagatctatt atgattttct ctactacaat
     5881 agtagctagt gggttattgt tattttcttt taatgtagat gtaataatgg gaattcctgc
     5941 agatttagta aaaggagatt atttgtttgg aagaacaata ggtattgtag aagatgaaaa
     6001 tggaaaacca gtatggataa tatctggcat ttggaagaca aatttatcga atcaaactca
     6061 agaagctacg agtaattcaa ctgtttttga tgcgtcattt gaaatgatca aaactgatgg
     6121 gacatcaaaa catactcaca ctatgactaa ttttgtctta gccgatactt tgaaccaaaa
     6181 taaaaacagt acaatattta acgggacaga aactattagt atgcctcaag gaccagttac
     6241 tgaagttcct ataagcattc aagtaatgaa taatagtctt ggtataataa acattgatcc
     6301 aaataaaata gataatcact ttggtgatga cccactgtat ggtattcctt tagaagagag
     6361 tgaacaatat aaaaagaaat cctaaacgat ctcaaataaa tttttttatt tttaattgta
     6421 gtaattttag taattatagg aatagttaat atataattta ttttgttaac aaagtatgaa
     6481 ttttaaaaat actgtttttt ttagtggtat ggccgtttta ctttcatttt tgattttaaa
     6541 tggtagtagt gtgacatacc aatcttcatt tgcaacagca acaacaacag aatatgaaca
     6601 caaaatgaaa tggggatcaa aaggtgttgc tactggtcaa tttaaccaag ctagtggaat
     6661 tgttgctgac tctgaaaata atatttatgt tgctgacttt accggtcgtt caaatatgat
     6721 tcaaaaattt acaaataacg gatcatttat ccttggtttc ggtatactag gtagtggtcc
     6781 tcaatttttc actaatccaa ctggtttaac tgctgactct gacaataata tctatattgc
     6841 tgattttgga aatcctacct atgcaattaa aaaatatagt agtgatggaa agtttataga
     6901 ttcatttggt tcctttggcc tgggagatgg agaatttatc actccaggtg gactaggtgt
     6961 agataaggaa aataatattt atgcaactga ttttggagaa aacaataatg tacaaaaatt
     7021 tgacaaaaat ggtaatttta taatgaaatt tggtacttat ggatctggtg acggaaaatt
     7081 tttgaatcca actgatgtag caatagattc acttggaaat atctttgtag ttgattcttt
     7141 taacgaacga gtacaaaaat ttgacaaaaa tggtaatttt ataatgaaat ttggtaaatc
     7201 aggtaccggt aacggagaat ttaatacacc tattgaatta gcaattgatt cctctgataa
     7261 tattttcgta gtagataatg gtaacaatcg tgtacaagta ttcaatagag atggtggata
     7321 tattactcaa tttggcaatc aaggtccaat ttctgacatt cttcaaggtc cggttggtat
     7381 tgcaatagat tctaatggta atgtatacat agcagatgga attgatagcg atatacatat
     7441 atttagtcaa gtaaattaat aattttttta ctttatattt cttagaaata gaattaaaat
     7501 ataatacata ctgtagaatt agcctaaaat tggcataaaa atatcttttg tattaattta
     7561 ttgttattta ttaaaatctt atataatatg aataaaataa aatgataata attactagta
     7621 atttaaacta aatattatta taatttactc ttctatatat tgcggccacc gggatttgaa
     7681 cttgtcatgt gtgggaatta acatactttt atttagcttt tttatttatt taattaatct
     7741 ttattcaaaa tactttattg attcataata ctttattgac taaaaatata aattcatgga
     7801 aaaaagtgcg ttttatattt cgaggttata ttataaaacc tattaaaatt ttgttgtagt
     7861 tattttaata atatggatga tattatatag gtaatattgc tcgattaggt ctatcatcga
     7921 gtatggcaaa acaaggtatg acaatagtat caaggttttt tcttcaaaat gcagaaccaa
     7981 acaaagcatc aggtctttta tcaatgctac catctagtat aacagatatc ttttcaaatg
     8041 atgaaaaaaa ggaattcact actagtcaat aaaaaatatc agaagatgaa gttattgaaa
     8101 taatatctaa tgaatgctgt aatggggata aacaaaaagg aaaaagggta tatgaagaag
     8161 cagtaaaaac tataaaggaa aattctggtc agtaaggaga aggattttta gatggtataa
     8221 taggtaattt taagaagaag ggagtttttt aagatttttt gtttgaaaca atcacaaaaa
     8281 tttaacagaa catatttttt tcgaaaagta atgaatcaat tttatccttt aagtttttta
     8341 ataaaatggg cttgtaaatt acatattttt cgatattggg aattttttcc tttaactgtt
     8401 ccaaatgtgc aatatcagca gtgattaaac atatacttgt attattatct atgttcttca
     8461 attgtttata catagtaatg ccatctatct gtggcatttt caagtcaagt aaaaccaaat
     8521 cataaagccc ttttttgaaa taatataaag catttactgg gtctgtataa gcgtctattt
     8581 gatatctacc atttttttct aaatatattt taaaaagatt attaatatcc ttatcatcat
     8641 ctattatcaa tactcttttt tttgccatgg acattttgcc taacttagtt caatagacag
     8701 cattatttat taagattgag gataattgct aattttttat atcttttagt aaatgaatat
     8761 tgacaacaaa atcctattga gaaaatttaa ttttaagaaa atttaaactc gattatttta
     8821 tgctattttt ttcttaatat caatagttag taattgctgt atttctgttt tcagtttgtc
     8881 aagatataca ggtttttcta atattttgtc aattttcata agatatgatt tatcataagg
     8941 aatattatct tttatataag cactaataat tatcgttttt attttacaat cagtatcttt
     9001 ttctcttact ttatttataa aatctaatcc tgacatttgc ggcattctat aatctgtaat
     9061 aatcaccgaa caattgtttg tatttttata aaaatattct aaagctttaa ctggattatc
     9121 aaaagaaatt gtattaaaac cacttgatgt taagtattca ctaaataagt caagaacgtc
     9181 tttttcatca tctaccaata agatgtaagt atctgtatgg ttagataatt ccattcctat
     9241 attgttattg ctattgaaat taataaacaa tactatttgt tattattatt ttataataat
     9301 taggcactat agcaatagaa gtaaataacg aaaaaatgtt aaaaatacat attcaataat
     9361 tatacttgtg tagtagtaaa ataaaatcta atttttaata aaacgattcg aatatttttg
     9421 gttttacaaa gattcactga ggctcaagca aagcatgcct gctagcctag ttttcggttc
     9481 aatgaactta ttaaatattt attgtaaatg gcttgtaaac tctgcgattg atataagtgt
     9541 aaagaaaaag actgtaaaat tacggttgat attataacat aaactttgta tatgaatttg
     9601 tataatattt tcagcctaat tgatgatggc attaaatcta ctaaaaatac ccctctgtaa
     9661 agtaagtatt gtatccataa acttgtcttt tgtgagtgca tctaaataaa ctatttttta
     9721 ataatatctc ttttcagatt atcaaagttg agaattaatt tatgttctag atttatttct
     9781 agtaaagaac gttgtaaatc ccaaagaatt ttaaatttat ttttgacata acgctataag
     9841 attgttctta taaaaatggt ataaaaattt attattttaa tcatcataaa gatattttat
     9901 aaataatgaa aaaaattatt acaaaattaa ttccttatat tttctcatgg ctttcattgg
     9961 gttttgttat atatgtattg gcaaaagcaa ttcatcacac tgtattaaaa taagataaat
    10021 ttattattga aaatataaaa aagcttgacc tcaccttttc cataatactt ttgggaaaaa
    10081 aggttggata attttttaat attgttacct gttacaatta tttccctatt caacacaaga
    10141 ttctacaaaa tctatgtacg tcacgtcact acaattgcta gtaatagtag caaatcacaa
    10201 ttgtctttct tatccatttc atttcctacc ctttcgtttt cctattcagg acgacttata
    10261 cgatgttttt cttaataaat atctgttaga ttatacgaat ttcgttatta aaatgattac
    10321 aacaagaatc gttaaatatt gaaagaactt gtatcttttt ttgtttgaca attcttgagt
    10381 tataaataca ctctttataa ttttgttttt gatacttaat ttttataggc ataaatgaat
    10441 gtttaatctt aacctaatac catcatttga gtaataaaaa gagtgtcaat atggttaata
    10501 aaaaaaaaca gtggagaggc aaagcaattt tacatgtcga tattaattca ttttactcat
    10561 cttgtgaaga gattaaagac ccctcactaa taggaaaacc tcatgcggtt ataatgacac
    10621 ctcagaacaa taacaacatc acaaaaggtg cagtaacaac atgttcttat gaagctaaaa
    10681 aacttgggat aaaatcagca atgccactat acaaagcatt agaattgtgc cctaatttaa
    10741 tattacatgc agtagacaaa aaattctatg aaaaaatatc aaacgaagtt atggaaatat
    10801 tagaagatta tgcagatatt tttgaacaag caagtataga tgaagcctat ttagagtgta
    10861 caaataaaat ttctaatact catttaacgg tagaagaata tgctcaagaa attaaaaaaa
    10921 ctataaaaga aaaatgtagt ggcttattaa catctatagg tatagcacca acaaaatctg
    10981 ttgcaaaaat tgcatctgat tatcaaaaac ctgacggtct tactaaagta aactttaatg
    11041 aattgaaaaa ttttcttagt ccattagatg tagaaacaat ttcaggaata ggaatcaaaa
    11101 cacaaaaaat tctaaaagaa caaatgaata tcaaaactat aggtcaacta tcaaatacag
    11161 atgttcaact cctcatagaa agatttggaa agaaaatagg aacatggatg tggcaagttg
    11221 caaatggttt agacaatgag ccagttcttc cacgcggaaa tcacatatca ttaagcaatg
    11281 aatctacatt agaatttgtt acagaggata aagagatgat aaagcgatct ctgta
//
LOCUS       DASVEV010000233         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_307676, whole genome shotgun sequence.
ACCESSION   DASVEV010000233 DASVEV010000000
VERSION     DASVEV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307676"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VF222_10315"
     CDS             <1..1414
                     /locus_tag="VF222_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142857.1"
                     /translation="MNNTNIANFLMTFSEFYLSYKYASTKKYDIIFMDRSLSNMYSSL
                     LYDTSNRKTWNTNSSILNYKIDDIPIDINDILIARHNIIYDKLNLPSPRGDYLRYAIF
                     NLLSQQNQEIDIGQIMILLKINNNEKIRKRIERYIHRSIDEGIIEEVKKRKYKIVERY
                     RFSWSRVKKLVTIISEKIFGEDKEHFILDNESGMRKWLTTLDLSFLTLFTLYMLIEEC
                     WKNKIILIGITKDTAAQEFKNHVIPICLTNNIWTNHDIISDNLNKIPNTDRMLLQVLS
                     MLNSDKINVPWGLVEYDSSFVMAIPDFEKRRGYVSGAIKNKITQSQLFLRSFVQLASS
                     RQDEKLRSNVLAIDRLVYPEFDLILPENIIEMKHEYGSTEKVKFLLFKNNKIKNDLQN
                     LLLIVLKSMSHSNIGETFGYNRPLFVADKIAKWHNQEFRKIVDSTSHLIACNKNLKNF
                     VYYMNTFREKRQGFESNRKL"
     gene            1392..>2662
                     /locus_tag="VF222_10320"
     CDS             1392..>2662
                     /locus_tag="VF222_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7142858.1"
                     /translation="MSQTENYNNNNILPIFTDFDGKFKGRLSAVIPARINNTSENAPI
                     SAKYDCRIKVEYHKEIMSLLEEGMIIAVKNFKSKFNNQQTITNCFSLFVISRIWPDHY
                     GMRGLSDSTYYPMQFEVIEQSVEDWATDDKSTMMIQISAIPINYDLLVNYTDDGEIKC
                     EYVKGFSYPIIGDTVFLLNAKTVSQMYNQKVLKKMNWDAFNNNCTNSEGPELGIIKMF
                     ENSPEKIPIYVHMDNLVRYHFGIFAFTGGGKSNLLSNLLRKILLHTKDTKILLFDISC
                     EYPFLLADLFADTSIESMIILERLVNDAEQFYVSVVKPREFEEDERAKQGLVKVFERG
                     IVTHFVKPSSVTPTCKEILKELEDLKNESTGKPHYLDAINQIYTKLFECMTQNNLNES
                     SFIDENFVQILSISAIEAMKTFKVSDKAGLYA"
BASE COUNT         1060 a          353 c          420 g          829 t
ORIGIN      
        1 aatgaacaat acaaatatag ccaattttct catgaccttt tcagaatttt atctatccta
       61 taaatacgca tctactaaaa agtatgacat tatattcatg gatagaagtt tatcaaatat
      121 gtattctagt ttgttgtacg acacatcaaa tagaaaaact tggaatacca actcctcaat
      181 actaaattat aaaatagatg acattccaat tgatattaat gatatcctaa tagcacgaca
      241 caatatcata tatgataagt taaacttacc ttctccaaga ggagattatc tgagatatgc
      301 aattttcaat ttattatcac agcaaaatca agaaatagat attggacaaa ttatgatact
      361 tcttaaaatc aacaataatg aaaaaataag aaaacgaatt gagagataca tacacaggtc
      421 gatagatgag ggaataatcg aggaggtaaa aaaaagaaag tacaaaattg ttgaaagata
      481 cagattttca tggtccagag ttaaaaaact tgttaccata ataagtgaaa aaatatttgg
      541 tgaagataaa gagcatttta tcttagataa tgaatcagga atgagaaaat ggttaactac
      601 actcgattta tcatttttaa cactgtttac actttatatg ttaatagaag aatgctggaa
      661 gaataaaatc attttaattg gaataacaaa agatactgca gctcaagaat tcaaaaatca
      721 tgtaataccg atttgtttaa cgaacaatat atggaccaat catgatataa tttctgacaa
      781 tctgaacaag atacctaata ctgatagaat gttacttcaa gttctgagta tgttaaactc
      841 tgataaaata aacgtaccct ggggtttagt agaatatgac tcgtcttttg taatggcaat
      901 cccagatttt gaaaaaagaa gaggatatgt tagtggggcg attaaaaata aaattacaca
      961 aagtcaatta tttctcagat cttttgtaca attagctagt tcaagacaag atgaaaaact
     1021 tagaagtaat gttttagcta ttgacagact tgtttatcca gaatttgatc ttatattacc
     1081 tgaaaatatt atagaaatga agcacgagta cggtagtaca gaaaaagtga aatttctcct
     1141 atttaaaaat aacaaaatta aaaatgacct tcaaaatctt ttacttattg tactaaaatc
     1201 aatgagccat tcgaatatag gtgaaacttt tggatataat agacctttat ttgttgcaga
     1261 taaaatagca aagtggcata atcaagaatt tagaaagatt gtagattcaa caagtcattt
     1321 gatagcatgc aataaaaatc tgaaaaactt tgtatattac atgaatacct ttagagagaa
     1381 gaggcaagga tttgagtcaa acagaaaatt ataataataa taatattctt cctattttta
     1441 cagattttga cgggaaattt aaaggaagat taagtgctgt aattcctgca agaataaata
     1501 atacatctga gaatgcacca atctctgcaa aatacgattg tagaattaaa gtagaatacc
     1561 ataaggaaat tatgagttta ttagaagaag gtatgattat agctgtcaag aactttaaat
     1621 caaaattcaa taaccaacaa actatcacca attgttttag tttatttgta atatctagaa
     1681 tatggcctga tcattacgga atgagaggat tatcagattc tacatattat ccaatgcaat
     1741 tcgaagttat cgaacaatcc gttgaagatt gggctacaga tgacaagtct actatgatga
     1801 tacaaataag tgcaattcca atcaattatg atttattagt aaattataca gatgacggag
     1861 aaatcaaatg tgaatatgtc aaagggttct cttatcctat aattggagat actgtatttc
     1921 ttctcaatgc aaagacagtt agtcaaatgt ataatcaaaa agtactaaaa aaaatgaact
     1981 gggatgcttt taacaataat tgtaccaact ctgaaggccc agaattagga ataataaaga
     2041 tgtttgaaaa ctctccagaa aaaattccta tttatgttca tatggataat ttagttcgat
     2101 atcattttgg tatctttgca tttactggtg gaggaaagag caatctactc tcaaacttat
     2161 taagaaagat attgttacat acaaaagata caaagatttt gttatttgat atctcttgtg
     2221 agtatccatt tttgcttgca gatctctttg ctgatacctc aatagaatca atgattattc
     2281 tcgaacgtct agtaaatgat gcagagcaat tttatgtttc tgttgtgaaa ccaagagagt
     2341 ttgaagaaga tgaaagagca aaacaaggat tagtaaaagt atttgaaaga ggaatagtta
     2401 cacattttgt taaaccttct tctgtaacac ccacgtgtaa agagatactt aaagaacttg
     2461 aggatttaaa gaatgaaagt acaggtaagc cacactactt ggatgctata aatcaaatct
     2521 atacaaaact ttttgaatgc atgacccaaa ataatcttaa tgaatcgagt tttattgatg
     2581 aaaattttgt acaaatttta agtatatctg caatagaagc aatgaaaacc ttcaaagtta
     2641 gcgataaagc aggattatat gc
//
LOCUS       DASVEV010000234         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1276990, whole genome shotgun sequence.
ACCESSION   DASVEV010000234 DASVEV010000000
VERSION     DASVEV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276990"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1282
                     /locus_tag="VF222_10325"
     CDS             314..1282
                     /locus_tag="VF222_10325"
                     /inference="COORDINATES: protein motif:HMM:NF037920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdvA-like protein"
                     /protein_id="HEX7142859.1"
                     /translation="MSELKLEFVGKQVKDMYGTLMGNVVGVITDTDGSIESVSVDCGE
                     PGLKQLSYEQLLIQGDYVIFIPKWRLDAQKLFRQKNLTLKRIKALQHLVEEDDTMKED
                     AEIVYAKYEKKLHELEEKEKSINEKLKERLGELNGSTKNIKSILFDAKLQYKSHEISE
                     ETYLQVTRYTKELMDHINLEKEEINNIKSRLQEQNLENTLPLPQSIQPDRKENEKENT
                     KLSTTNTNSVSPTITNTPNTTTTTTTTAVTASNTNNSNSSNTSLPVSSTSNDNTQTKL
                     SYAGETDKHKQHASYNNTDHSNLNKDKIEKSNTDNESNWLDHVINN"
     gene            complement(1399..1659)
                     /locus_tag="VF222_10330"
     CDS             complement(1399..1659)
                     /locus_tag="VF222_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142860.1"
                     /translation="MKYRSRTEIVAMILEAANGGATKTKIMYKAFLSYAQLREYLSVL
                     IENNLLEYLDGTQTYKTTEKGLNFLKMHSEIGELLQTPATHL"
     gene            1894..>2089
                     /locus_tag="VF222_10335"
     CDS             1894..>2089
                     /locus_tag="VF222_10335"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7142861.1"
                     /translation="MSIDNDSSSSDTFFTSSNLKITLEKNNFQAKRAQSLQNIEAFWA
                     EQAENLVWNRQWNKTLEWNPP"
BASE COUNT          853 a          281 c          272 g          683 t
ORIGIN      
        1 tagttctaag tgtatatcta taacctatga tatattttga tatgtggatt ttttctttag
       61 caatttgagt gatataatca ggattcccag gtcctacacc aataacaaac aatttctttg
      121 gcatttgaaa ttgtattaca agttataata gctaataata ttcttttata aaagaattag
      181 atccatatat ttaaaaatta aaatgatttg gtggaaaaag aaacgatatg actagtggaa
      241 aagaaacgat atgactaaat gacataacta ttttgacatt cttatcatta tatgtaatta
      301 ttaacggatt tgcatgagcg aacttaagct cgaatttgta ggtaaacaag ttaaagatat
      361 gtatggcaca ttaatgggaa atgttgtagg agttattact gatactgatg gttcaattga
      421 atctgttagt gtagattgtg gtgaaccagg tttaaaacaa ttatcttatg aacaactatt
      481 gatacaaggt gattatgtaa tcttcatccc caaatggaga ttagatgcac aaaaattatt
      541 taggcaaaaa aatttaacgc ttaaaagaat taaagctctt caacatttag ttgaagagga
      601 tgataccatg aaagaagatg cagaaattgt atatgctaaa tatgaaaaga aactacacga
      661 attagaagaa aaagaaaaat ctattaatga aaaactaaaa gagagacttg gtgaactgaa
      721 tggatcaaca aaaaatatta aatcaatttt atttgatgca aagttacaat acaaaagtca
      781 tgaaatatca gaagaaacat atctacaagt tacaagatat actaaagaat tgatggatca
      841 tattaattta gaaaaagaag aaatcaataa tataaaaagt cgacttcaag aacaaaacct
      901 agaaaacaca ttacctttgc cacaatcaat tcaacccgat aggaaagaaa atgaaaaaga
      961 aaacacaaaa ttatctacta ccaatacaaa ttccgttagt cctactataa ctaatactcc
     1021 caatacaaca acaacaacaa caacaactgc tgttactgca tccaatacaa ataattctaa
     1081 ttcttcaaat acatcattac ctgtatcctc tacatcaaat gataatacac aaactaaact
     1141 ttcctatgct ggtgaaactg ataaacataa acaacatgca tcatataata atacagatca
     1201 ctccaatcta aataaagata agatagaaaa aagtaataca gataacgaat ctaattggct
     1261 agatcatgtt ataaacaact aatttttatt ttttaatttt atacatctag atttaattat
     1321 ttatcttaaa gatttttata ttaacataaa gaatttaaag aaaaaaaacg ataaaaataa
     1381 aaaaaattaa tagtgaattt ataagtgtgt ggcgggagtt tgtaataatt ctccaatttc
     1441 actatgcatt ttaagaaagt ttaatccttt ttctgtagtt ttataggtct gagttccatc
     1501 aagatattct aatagattgt tctcaatcaa cacagaaaga tattctctta attgtgcata
     1561 acttaggaaa gctttgtaca taatttttgt cttggttgct cctccattag ctgcctctag
     1621 tatcattgct actatttctg ttctacttct gtatttcacg atcatcatac acaatattta
     1681 tttattttaa tatattgaca aaatgtagta taacattatt ctgtactaaa actcaatgtc
     1741 ataattattt atgcaatcat atattaacta atttgttaaa taaacttgtt tagatattaa
     1801 tatatagaac atataaatag tttagtcaaa taatcttaac ttacatttat tatataatta
     1861 caaatttaac tagaagtaac tatatcatat attatgtcaa ttgataatga cagttcatca
     1921 tctgacacat tttttacaag ttcgaatttg aaaattacat tagagaaaaa taattttcaa
     1981 gctaaaagag cacaatcatt acaaaatatt gaagcatttt gggcagaaca agctgaaaat
     2041 ttagtatgga atagacagtg gaataagact ttggagtgga atcctccat
//
LOCUS       DASVEV010000235         6238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1278490, whole genome shotgun sequence.
ACCESSION   DASVEV010000235 DASVEV010000000
VERSION     DASVEV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278490"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..301
                     /locus_tag="VF222_10340"
     CDS             44..301
                     /locus_tag="VF222_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142862.1"
                     /translation="MIGFNAYKKSINDIFILIEKLSKQYKQFEPDLKNIQTLNEQELL
                     IIDGEKGYLTYTNLDLDKLENFLKRLLTDKDFIKNYFCKII"
     gene            complement(298..549)
                     /locus_tag="VF222_10345"
     CDS             complement(298..549)
                     /locus_tag="VF222_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142863.1"
                     /translation="MSTADIPLNDSNIQDIIKKEARGLGDNTDFGEVQEILGEHVITQ
                     KGNVAKDRYYIPRNLIERFDGNTVYFKITKDESKQYKRD"
     gene            complement(975..1121)
                     /locus_tag="VF222_10350"
     CDS             complement(975..1121)
                     /locus_tag="VF222_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142864.1"
                     /translation="MTNDNDKDIKIETGEDVGKDDKIKAGVKALAKKPFRETSAEYKA
                     DSLE"
     gene            complement(1547..2356)
                     /locus_tag="VF222_10355"
     CDS             complement(1547..2356)
                     /locus_tag="VF222_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142865.1"
                     /translation="MSDKKIGIFSALLFIGVATLFVPVTALQNSIAMAQEYYPEYEEN
                     EYYYDQEGYDLYTNNNDNKNALIVNVEKKLFVCNNATDTPNDFECEGFREFFGDGDFI
                     SYPPGPDSGQYIPCNDEICPFIDESDFGVQIFKDVATVRELSPEGNTVNLDKLHYTVT
                     ELFITDRIPTNDQCSLVGFTHSLFPQKIANGSIIFNGICVNYVGDCEGTIYPGEVKTC
                     TVENYIYERHFDPIQTVGNALDGTQSSLLSQQFNNQAIQSQPKMLKDLMSN"
     gene            3858..4082
                     /locus_tag="VF222_10360"
     CDS             3858..4082
                     /locus_tag="VF222_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142866.1"
                     /translation="MSDNDNDEDTSKKVFDYNINENQFAIEYTAVFEDGTSDSYIENK
                     IIRGTAAERLPIWVDSIKKKEEKSKKKSNK"
     gene            complement(4683..5510)
                     /locus_tag="VF222_10365"
     CDS             complement(4683..5510)
                     /locus_tag="VF222_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7142867.1"
                     /translation="MLLLVVTVILLTLTITLVIPSVDGQTEDPLPVLLVHGYYEGPEV
                     WDAWLNELQDDGITAEAVEFEFDDQCGTSASHAQELVDIVNRFKEDSQADKINIVAHS
                     KGGLDARLYLANDSTNTDIANLIMIGTPNHGSPLVWGVSLINPFTIPPLMLPIVERFF
                     CLPAASDLKPGSEATLSVKNANTKYYTIAGSWKPDFYFSNVFSFSSDSNCLQASWLPL
                     QRWGSDFVILGQDDGLVPIRSAMPTEFRNLGISDDCHTNLLSEQLEYDWAKQILLPE"
BASE COUNT         2275 a          827 c          852 g         2284 t
ORIGIN      
        1 caaatacatg tattatcttt gaatactatg tcatttaata aagttgattg gattcaatgc
       61 atacaagaaa tccattaatg atatctttat ccttatagaa aaactttcaa aacaatataa
      121 acaattcgaa cctgatttaa aaaatattca aactcttaat gaacaggaac tcttgatcat
      181 tgatggtgag aaaggttatc taacatatac aaatttggat ttagataaat tagaaaattt
      241 tcttaaacgt cttttaacag ataaagattt tataaaaaat tatttttgta aaataattta
      301 atcccttttg tattgttttg attcatcttt cgttatctta aaatagacag tattgccatc
      361 aaatcgttct atcaaatttc taggaatata atatctgtct ttggctacat ttcctttttg
      421 tgttattaca tgttctccta aaatctcttg aacttcacca aaatcagtat tatctcccag
      481 ccctcttgct tcttttttta taatgtcttg tatattagag tcatttagag gaatatcagc
      541 agttgacatg ttctctatgg ttcatcatta tatatattga ctacaattaa agcaaaggaa
      601 tgttttttaa ataaaataaa taaatatcaa ttgttctttt aaaattttaa aaacaaggtt
      661 ttaattaaaa atgattttta taacttggga aatttttatt agattagtat aatcacaata
      721 ccttaataat aattataaat taaatttgaa ttaagttaat gtcttattta tgattcaata
      781 ttttgtaata ctttttttga tttttttctg atgcaaaaaa gaataactaa attattagtt
      841 atcatttttg aaaatatctc tttgtataaa atatagtaaa acagtatgaa tgatattctc
      901 tatatatcta tgatatatca ctagagtaat agattaatta gtcttaatat aacaataaaa
      961 taaaatatat atgtttattc tagtgaatct gctttatact ctgcactagt ctctctaaaa
     1021 ggttttttgg ctaaggcttt tacacctgct tttatcttat catcttttcc tacatcttca
     1081 cctgtttcaa tttttatatc tttgtcatta tcgtttgtca ttgtaattac cactacgtta
     1141 caaactattt attgattaga ttagatttat aaaattaaga ttaattttgg agaaatattt
     1201 agtccagagg tttgtgaatt gtgattgaca caatattttt ggcccacatt cgtagagaga
     1261 attaaatctt tgcatataaa ttgcgtaact aatacctcct aatccaattt tgaaacataa
     1321 ccaagacaaa taatggttta atattaatag ataaataatc aatataaccg aaaaatgatt
     1381 ggtattatca ccatgcagga aaatcattct atagaagatt attttatgcc tatcttgtaa
     1441 taaaagtaat caatattctt tattaggtga tctaattttg attaatcttg gctagtaatt
     1501 tttttttagt gctaaaaaaa ataattgttt gtttttgtta atcacttcaa ttagacatta
     1561 aatcttttaa cattttaggt tgagattgaa ttgcttgatt gttgaactgt tgtgataata
     1621 atgatgattg tgtaccatct aaagcattac caacagtttg tatagggtca aaatgtcttt
     1681 cataaatgta gttctctact gtacatgtct ttacttcacc aggatatata gttccctcac
     1741 aatctcccac ataattgaca catataccat taaatattat actaccattt gcgatttttt
     1801 gggggaataa actatgtgta aaaccaacaa gagaacattg atcattggtt ggtattctat
     1861 cagttatgaa cagttcagtt actgtatagt ggagcttgtc aaggtttact gtattacctt
     1921 caggtgataa ttcgcgtacg gttgcaacat ctttaaaaat ttgaacacca aagtctgatt
     1981 catctataaa tggacatatt tcatcattac atggtatata ttgaccgctg tctggtcctg
     2041 gaggatatga aatgaagtca ccatctccaa agaattcgcg aaaaccttca cattcgaagt
     2101 catttggagt atcggttgca ttattacata cgaataattt tttctctaca ttgactatta
     2161 gagcattttt attatcatta ttatttgtgt atagatcata tccttcttga tcataatagt
     2221 attcattttc ttcatattca ggataatatt cttgtgccat tgcaatagaa ttttgaagtg
     2281 ctgttactgg aacaaacagt gttgctactc caatgaataa caatgcgctg aaaatcccta
     2341 tttttttatc tgacatgata atgaaaacat attaatatat gtatatatta gtaaagtaga
     2401 aacaatcagt tctaatattc aatatgaata ttagaagaga gaaatccaaa atattctata
     2461 tagttatttc tagtattttt tatatctatt ttaataatag aaagtaatgc aatataatga
     2521 gtaaggcaaa ataaaagcat tgttatcaaa tccggctcag aggtttgacg gtcatttttg
     2581 cctgtaatcc taatcaataa agatttacaa attcattggg ctcagaggtt tgtgaattgt
     2641 tattgacaca atattttttg gccctggtgt ataacaaata tcagataatg tatctaataa
     2701 attatgatat tatgaatata cttttctatg ttgaataaat cttctagaaa tggtcacaaa
     2761 gtacgtttat actttaccaa aaaagagata gaagttttta gataaagtgt ttggttctaa
     2821 ttcgggtaat cttattacta atcatcattc atatttcata atctttctat tactttttga
     2881 gctccaagaa atgaagatgc atcattctgg atttaataac aaaattagaa tctgttaaat
     2941 ttgcgttcaa aagtgatcca gacaaaaaaa ataggattta tagcggaaat agttcctgat
     3001 aattgttcaa tcaatcattt aggatttatg atttcatata ccatttacga cgtactaaat
     3061 actctcaact gaaactattc tcgcataaac ataaatactt aataagagat taaaaaaata
     3121 ttatttaata aaacattatt tctttaaaaa gttctagtat ataatacgga aactagtttt
     3181 aataaacctt atataattaa gataaaaata acgttaataa taatatatga cttattcaaa
     3241 cggcaaaagt aaattaacat cagcaacatt aaatctacag aatacaattt ataataataa
     3301 agtatctgtt cttactaata catttttaaa ttgtaatact tgcagaaaaa gtattcagaa
     3361 aaatccaatc ctctgttata tcttcataga ctatttttgt agtgaaaaat gccatcttca
     3421 taaacataaa atacatttgg aaaaaatcgt taatgtagat taaaatattc ttttgatgta
     3481 ttttaaaata atattcataa aaaaattcaa taatataaaa tcaaaaataa tttcttatgg
     3541 tttttcatta ttttcaattg tttttgttgt gctatatatt gataagtgca aaattaatca
     3601 ctatctttct aaataattaa tttaacacat atttttagta aattttgatt ctgtcgaata
     3661 tggatgtttt aaattagtgt tgaaaagtgg atgttatgca gaggcacaat atataacagc
     3721 cacttagaac taaactgtat ttattattga cttggacttt gtagcagcta ttatactaat
     3781 agaataagta taataaatca attgttaatt tttggtaaga agcgaattta tttgagtaat
     3841 ttgtacctgt tctacagttg tcagacaatg ataatgatga ggatacttca aaaaaagttt
     3901 ttgattataa cataaatgaa aatcaatttg caatagaata tactgctgta tttgaagatg
     3961 gaacttctga ttcttatatt gaaaataaaa taattagagg aacagcagca gaaagacttc
     4021 ctatttgggt tgattctatc aaaaagaagg aagagaaatc taaaaaaaaa tcaaacaaat
     4081 aatagttaat ttttatatca aataataaaa aacggtattt taggacttga tttaaagatg
     4141 aacatcaaaa aaagaatacg acctaaaata catcctacct acatttggag tagagttctt
     4201 gcatacagaa gagttctact ccgaatctgc aattagcttt tattattagt aacttatttt
     4261 tatattgttg ttaaaagtga aaataggtaa atttattaaa aatttgtgca ggagataaat
     4321 accgatagtg gtacaaaaag agtaaaaata gcaatatgga gactatttaa gttttctcaa
     4381 tagaattaaa ggctcataaa tctcatgatt tttatcaata ccaagcaatt caattataga
     4441 atctcaaaat aaattcatat ttttcggcgc ctaaaaattt ccaacaaaaa tttaatactt
     4501 aataggatgg ctggaatgac tcagattgca tcctatttcc attacacagc tattgtgtaa
     4561 tgatattaaa tatatggtat ggtattattt aggatatttg gtaaaaaaat tatttaagta
     4621 tattagtttt tgaaactaaa gatctatcca aaaatataaa tatattttct aattttattt
     4681 gcctattctg gtaataatat ttgttttgcc cagtcatatt ctagttgttc agataaaagg
     4741 ttagtatgac aatcatcaga tattcccaaa tttctaaatt ctgtgggcat agcacttctt
     4801 attggtacca atccatcatc ttgtcctaaa ataacaaaat cactacccca tcgttggaga
     4861 ggtagccatg aggcttgaag acaatttgaa tcagaagaaa aagagaaaac gttagaaaaa
     4921 tagaaatcag gtttccaact tccagcaata gtataatatt tggtatttgc attttttacc
     4981 gataaagtag cttccgatcc tggctttaaa tcagaggctg ccggtagaca gaaaaaacgt
     5041 tccactattg gaagcattag tggtggtatt gtaaaaggat ttatcagtga aactccccat
     5101 accaatggtg agccatgatt tggtgttcca atcattataa gatttgcaat atctgtatta
     5161 gtactatcat ttgccaaata taaccgggca tctaatccac ctttactatg ggcaacaatg
     5221 tttattttgt ctgcttgact atcctcttta aatctgttaa caatatctac taattcttgt
     5281 gcatgtgatg cagaagttcc acattgatca tcaaactcaa attcaactgc ttctgcggtt
     5341 attccgtcat cttgaagttc atttaaccat gcatcccaaa cttcaggacc ttcataataa
     5401 ccatgtacta aaagcactgg aagaggatct tcagtttggc catctacaga aggaattact
     5461 aatgtaatag ttaaagtgag tagtattaca gttactacca ataataacat attaaatcta
     5521 atcataaaaa tataaaatga ttcaaagtat ttattattat ttatcaatat aaaatagatt
     5581 tagattgaca gtaaatatcc tttctgaaca ttaaatagta acatagtaca aagaagatat
     5641 atttatctct tgaacgaaca tatgttgatg atgtttaggt gaaggtagaa gacaccaact
     5701 ttacgtgata ttctttattc aaagttatta ttttatataa ctacatacac actattaact
     5761 ttaaatgaca agttaagtta taatgtatta ctacactttt taaataaaaa ttgatattgc
     5821 ctggatggtt ttacggtcat atttgcctgt aatccaataa tcaataaaat taacaaattc
     5881 attgggctca ggttctgaac tttactaata attaggttca atatactttt gaaacttaat
     5941 attataatct atatgataga caagtaataa attttcaagg tattagaatt ttatgctaaa
     6001 tatttattgt taattaatta aaataatttt tgaatattag aataaataat aaaataaaaa
     6061 aatgaaggag aattagggaa tgtggggtgg tggattgtca aaacattgag cgaatgtaat
     6121 aatattaaca gcatcaagat ttgttgttgg tccgagtgca cttgttatcc agccgtcttg
     6181 cgtaggtagg gcattgtctt gaatagaagt tgtcctgtta gatccaacag cggagtaa
//
LOCUS       DASVEV010000236         6896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1279918, whole genome shotgun sequence.
ACCESSION   DASVEV010000236 DASVEV010000000
VERSION     DASVEV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279918"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..626)
                     /locus_tag="VF222_10370"
     CDS             complement(273..626)
                     /locus_tag="VF222_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142868.1"
                     /translation="MVDDEEDILFTYKIFLEEHDYNVTSFTDPSFAMNYIRNIPNFKN
                     LLVILDIRMKNLNGFQLHQQIKAIDPTIKVLFITALDILDEFSTIIPGISEEYIMKKP
                     VDRKIFTSTIQKILK"
     gene            complement(1417..1605)
                     /locus_tag="VF222_10375"
     CDS             complement(1417..1605)
                     /locus_tag="VF222_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142869.1"
                     /translation="MTIMMVAITTTISQTHEVYADNKAKDLCSQYDGELNNGKCKIKM
                     MKIKQIMKMSCVMTIKIQ"
     gene            complement(1826..3022)
                     /locus_tag="VF222_10380"
     CDS             complement(1826..3022)
                     /locus_tag="VF222_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7142870.1"
                     /translation="MTISSYIINNITLRPVNVLYEAVTIREEMKKMPEEYLWIHANEI
                     EDIFPLQDIFGLHPLTVEAVLHQNQPSKIEEYTRYIFAIIDGIADKQEKKIEESDNKN
                     DRKILSMMILEDDLYMFLEQRWIITLNVYNQHFEENIKKNIMAIIQQTKRTMPNSTAF
                     SVNKKIFDSNKNDRFKKNEMIFRLALEEMILSYFPILDNLGKDLEKTEEDILDENSGS
                     SSKDNKSQLSKILLLRKKINFIERTLGMMSRAVQDFVNRNNFVNTSDNETLEYFTVSQ
                     TSSVKRDEKSTVLLTPDSIRHMRSLNDRIIYLRNDIENMHQRIISLRETYNSSLSANL
                     NETIRTLTVIATIVLPLTLITGIYGMNFEFMPELASEFGYYYALGLIAALGGGMVLYF
                     KRKRWI"
     gene            complement(3402..5819)
                     /locus_tag="VF222_10385"
     CDS             complement(3402..5819)
                     /locus_tag="VF222_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7142871.1"
                     /translation="MSDIREPESPIHPHEHKLVRSLGLLDVVMIGIAGMIGGSIFVLT
                     GPAIGLAGSSVILAFILNAIITLFTAMGYAELGSAMPEAGGGYLWVREGLRRPNAFIS
                     GWMAWLAHIIAGSLYAVGFGSFFISFLQLTNILPLESLFGIIPLDKLTAVAVVVAFTF
                     INIKGASETGKVGTVITLVQLGTIVSIIIAGLWTMSSHPNDDWQSNFTDFLPMGLGGL
                     VAAMGLTFIAFEGYEIIVQTGEEVKNPKRNIPRAIFISLALVVIMYCLIAFVSIGAIF
                     PDMPSWQFIGLHGELGIMKAAESFMSYGAFIVLAGGLVSTLAALNATTFSSARVAFAM
                     GRHYNLPHRLSAIHPKFKTPYVSVILSSIIMAVMAYALPLEDIAHASAVIFLLLFTQV
                     NLAVISIRRMYGNKLDYGYKTPFFPYVSIIGIILMVGLAIYLLITAPFSWAITVLWVL
                     VGFFIYRIFTFKKEVEHYSPTITSEGNLERKDFRILLPYTPENPDRLIRYAIKVAKEK
                     DGEINILRTITVPHQTPLSAGIAFTDSARKAFDSLEEILNKEDVIYHYYVRISHDATE
                     AVLTTISEQKIDLMIIDYETMRSNKKLQTLLTCDVLAIIAHSEDFLENQNNPNETIVT
                     KEHKKNMVVLYDDGDNSDELLRVTSWFINIERFNLNVIALNRKGLNNSNKSNPKSKES
                     IHDENASIFTKRRKFFQEAGVELNEINVTDDVEKDSPQFAKLILEWIMRYNPDIVITE
                     STIGKYNLFTNSKFASLLLSQLNYPIIIVRDSTMPLVSLVTHLMLKITGNQGPAHLVR
                     LMRNKTK"
BASE COUNT         2447 a         1032 c          944 g         2473 t
ORIGIN      
        1 gtactcagtt atggaagata aattaaaatt tcttgtacat ggtagaaaaa aggcaatgaa
       61 ttcgtcttgg aagaaattac aaactatata ctaaaaaatt tacaatccta tgatgataaa
      121 accattatag taacatttga actccctgtc acaaacaatg tgataaaaat ggatagttgt
      181 attctaatca attgattaag taattatctt ttgaaatatt aagcaaagat ttactttata
      241 tcacaataat tgataatgaa atataatttt tattatttca aaattttttg tatagtactt
      301 gtgaatatct ttctatcaac gggttttttc attatatatt cttctgagat tcctggaatt
      361 attgttgaaa attcatctaa tatatctaaa gctgttataa agagaacctt aattgtcggg
      421 tctatagctt ttatttgctg atgtaattga aatccattca aatttttcat tcgaatatca
      481 agtattacta ataaattttt aaaattcgga atatttctga tatagttcat agcaaaagag
      541 ggatcggtaa atgaagttac attataatca tgctcctcta aaaatatctt ataagtaaaa
      601 agaatatctt cttcatcatc aaccaaaatt atccttttgt tattattatt taagttccca
      661 attgaggaac ctaattgttg ttcatcaaag gaatttttat cattaacctc aatatcatta
      721 agcccagaaa tatcttttct tttaatatta tatagagaat atctattact ttctgttatt
      781 tgataattga tatgatttat aaaattataa ctatttagta tagtaggaaa agattttaaa
      841 attctataga aattatctcc aataattatt tcatttgcaa tagacgaaga agaagaagcg
      901 tttatctttg aacaaaggtt tatagttgtt ccaaataaat ctatttgact ataatctcct
      961 actaaagcta aatctagtac tccataatct atagtaatcc tataattgaa tggtggaaac
     1021 tgttgctttg ataattcttg attaatacta tatcgttcat ctaaaatttt aaatccacat
     1081 tcaataacct ttctaaagac tcctatatta ttatcagtag ttttgggaaa gtaaaataat
     1141 aaacagtcac caatattttt aaaacttaat aatatgctta tatttaatta tctataaaac
     1201 aatttaaatt acttaaattc actaatatat ttagatataa atatttaaaa aaagggtcca
     1261 tcaggagtgg attcgaaatt agagaaaggg agagaagacg aaacatttta ttttgaaata
     1321 agaatacttg aattacttat aaaataaaaa tagtaaattt aaaaatacga ggttaattta
     1381 tagctagtca gaattcttta catttttttg catatttcat tgtatcttta tggtcatcac
     1441 acaactcatc ttcataatct gttttatctt catcattttt attttacatt taccattgtt
     1501 caattctcca tcatactggg aacataagtc tttagcctta ttatctgcat atacttcatg
     1561 tgtttgtgag atagtagtag ttattgcaac catcattatt gtcataatta agaatattaa
     1621 tgcttttcat tttattatca ataattaatc aataatataa agaagtttga gaaaagaatg
     1681 aggaaaaagt tttaaatttt ttgaaggtag gattggaacc cagagttcaa tgtagccagt
     1741 gggatatgaa acaaatttga cctaaatata tggatttaaa tttgtcagta ttaattgctt
     1801 atagaaataa gtattattct caattttaaa tccatctttt tcttttaaaa tacaaaacca
     1861 tgcctcctcc aagagctgct attaagccta gagcataata atatccaaat tcagatgcta
     1921 attcaggcat gaattcaaaa ttcattccat atattccagt aattaatgtc aatggtaata
     1981 caatggttgc aattactgtc aaagttctaa ttgtttcatt caaattggca cttaacgaag
     2041 aattataggt ttctctcaaa ctaattattc tttgatgcat attttcaata tcgtttctta
     2101 agtagataat tctatcattt aatgaacgca tatgtcttat tgaatcagga gtaagaagca
     2161 ctgtagattt ttcatccctt tttactgatg atgtttgaga aacagtgaaa tattccaagg
     2221 tttcattgtc actagtattt acaaaattat ttctgttaac aaaatcttga acggctcgtg
     2281 acatcattcc caatgtacgt tctataaaat taatcttttt tcgaagtaaa agaattttag
     2341 ataattgaga tttattatct ttactagagc taccactatt ttcatctaaa atatcctctt
     2401 ctgttttttc aaggtcttta cctaggttgt cgagaattgg aaagtaactt aatatcattt
     2461 cttctaatgc aagtctaaat atcatctcat tttttttaaa tctatcattt ttattactat
     2521 cgaatatttt cttatttaca gaaaaagccg ttgagttagg catagtccgt tttgtttgtt
     2581 gtataatagc catgatgttt ttctttatat tttcctcaaa atgttgatta taaacattta
     2641 atgttattat ccatctttgt tcaagaaaca tatataaatc atcttctaaa atcatcattg
     2701 acaatatttt tctatcattt ttattatcac tttcttctat tttcttttcc tgtttatctg
     2761 caattccatc aattatggca aaaatatatc gagtatattc ttcaatctta gatggttgat
     2821 tctgatgcaa aacagcttcc actgttagtg gatgaagccc aaagatatct tgtaatggaa
     2881 atatatcttc aatctcgtta gcatgaatcc ataaatattc ttctggcatt tttttcattt
     2941 cctctctaat ggtgactgct tcatagagta catttactgg tcttaatgtt atattattta
     3001 taatgtaaga tgagatggtc aattattata atatttatga acatcaatta aataaactat
     3061 ctggaaaatt tgaccatgcg cggattagga agaatcacct atatttttgg gcagcaatgg
     3121 gtagaatatt ttaaattgtt atagtaatga gaacaggact taaaaaaggt taagatagat
     3181 aataaagtga agtgtatgaa tttgaaaata ctacatgctc gtcggtttgc aacctttttt
     3241 gctcctattt tttagagagg gaaaaccaaa tttgaccaaa taggaaattt gaagttattc
     3301 atattttgag tatctttata gatgctttat taggataatt ttgagaaaaa taaaggaatg
     3361 aattttatac tatgatatat ttactaaatc aatgtaaaaa tctattttgt cttatttctc
     3421 attaaacgca caagatgagc aggaccttga ttaccagtta tcttcaacat gagatgtgtc
     3481 acaagactga ctaatggcat agtagaatcc cttacaatta ttatcggata attcaattga
     3541 gatagcaata aactagcaaa ttttgaatta gtaaatagat tatattttcc tatagttgat
     3601 tcagttatta ctatatctgg attgtatctc ataatccatt ctaatatcaa ttttgcaaat
     3661 tgtgggctat ctttttcaac atcgtctgtg acattaattt catttagttc aactcctgct
     3721 tcttggaaaa atttgcgtct tttcgtaaaa atagaagcat tctcatcatg tatagattcc
     3781 ttagatttag gattgctttt gttagaatta ttcaaccctt ttctattcaa cgcaattaca
     3841 ttcaaattga atctttcaat atttatgaac caacttgtca ccctcaataa ttcatcagaa
     3901 ttatccccat catcgtatag aacaaccata ttttttttat gctctttggt tactattgtt
     3961 tcattaggat tattttgatt ctctagaaag tcttcactat gagcgataat tgccaaaaca
     4021 tcacatgtaa gcaatgtctg taattttttg ttacttctca ttgtttcata atctataatc
     4081 attaaatcaa ttttttgttc tgatattgtt gttagaactg cttctgtggc atcatgagaa
     4141 attctaacat aataatgata tatgacatct tctttattca atatctcttc aagagaatca
     4201 aatgcctttc ttgctgaatc tgtaaaggca attcctgcag atagaggtgt ttgatgtgga
     4261 actgttattg ttcttaagat attgatttca ccatcttttt cttttgcaac ttttatggca
     4321 tatcttatta gtctatctgg attttctggt gtgtaaggca gtagaattct aaagtctttt
     4381 ctttctaggt tgccttcact tgttattgtt ggagaataat gttctacttc ttttttaaac
     4441 gtaaagatac gatagataaa gaaaccgacc aaaacccata atactgtaat agcccagcta
     4501 aatggtgcag taattaaaag gtatattgcc aaacctacca ttaagattat tcctatgatg
     4561 gacacatatg gaaagaatgg tgttttataa ccataatcaa gtttatttcc atacattctt
     4621 cttatactaa ttacagcaag attcacctgt gtaaataaaa gcaaaaatat tactgcagaa
     4681 gcatgagcta tatcttcaag agggagagca taagccatta ctgccataat aatacttgac
     4741 aaaataacag aaacataagg tgttttaaat ttgggatgaa tagcacttaa tctatgaggt
     4801 agattataat gtctgcccat tgcaaaagca actcttgctg aagaaaaggt tgttgcattt
     4861 aatgcagcaa gagtagatac taatcctcct gctaaaacta tgaatgctcc atatgacata
     4921 aatgattctg ctgccttcat tatacctaat tctccatgta gaccaatgaa ttgccatgaa
     4981 ggcatatcag gaaaaatggc accaatagaa acaaaagcaa taaggcaata catgatgaca
     5041 actagagcaa gtgagatgaa aattgctctt ggaatgtttc ttttaggatt tttaacttct
     5101 tctcctgttt gaactattat ttcatatcct tcaaatgcaa taaaagttaa acccattgca
     5161 gctacaaggc cacccagacc cataggtaaa aagtcggtaa agttagattg ccagtcatca
     5221 ttaggatgag aagacatagt ccacaatcct gcaattataa ttgagacaat tgttcctagc
     5281 tgaactagtg ttattacagt accaactttt cctgtctctg aagccccctt gatgttgata
     5341 aaagtaaatg ctactaccac agcaacagca gttaacttat ctaatggtat tattccaaaa
     5401 agactttcta gaggaagaat atttgtcaac tgtaaaaaac ttataaaaaa cgagccaaaa
     5461 ccaactgcat acaaacttcc tgcaataata tgagccagcc atgccatcca cccactaata
     5521 aaagcattag gacgtcgtaa tccttctctt acccataaat atcctccacc agcttctggc
     5581 attgctgacc ctaactcggc gtaacccata gcagtaaaaa gagtaataat tgcattaaga
     5641 ataaaagcca gaatcacaga gcttcctgca agtccaattg caggtcctgt aagtacgaat
     5701 attgaacctc caatcatacc tgctattcca atcattacca catcaagcaa acccaatgaa
     5761 cgaacgagct tgtgttcgtg aggatggata ggactttctg gttctctgat atcagacatc
     5821 tattgggtct ctagtaatat ccactatcaa atatatgtat acgaaaacaa aatttgaatt
     5881 tataaactac caatttggag gaatgggaga ataataaata tagaaaatta tgaataccca
     5941 tcattttaga aggctgtcta taattaacaa tgaataaaaa atagtagaaa tactcactga
     6001 ttaagatata tttaaagcga ttttcagtaa taaagacttg ttatctgatt attataacaa
     6061 taagaatttt caaccttcat attctatctt tgaagaattt cgagatcgta tttttgaaac
     6121 tatttgtaaa ccttaaacaa aagtattaca aggagtaata attgctatat ctgggtattt
     6181 gatatacaaa ataaagacag gtaataaaca ggaggagatt ataacaaaga cataaacgat
     6241 aaaataaacc tcattcattt caaagatagc tctggccata attataaaat ataatacctt
     6301 ctttgcttta aaaattaggt ataatgattg gaaaaagcaa agagattctc ctcagagtat
     6361 actatgttat ccatcattga atacctttgc ttatattcca gaaaaacacc aattagcaaa
     6421 tataacataa tgacaaaaaa ttagtgaaat taaacaacaa cgtccagata gaatttcttt
     6481 gatcctaaat aaactagaac aaaactgcta tctaaaatca atcgatacac ctaatgcaaa
     6541 ataacaaatg gtaacaatat gattatatta tttattaaaa taagagtcaa ttgtatggta
     6601 atagagaata atatttaaga ttttttagtg tgaatgttaa tcttaatgat aataaaatgc
     6661 ttatcttggc atttaatgat aaagatttag tagatggaag aaagattatt atgatttctc
     6721 catttaatct atgtattctt tatttaaaga aatactacat tttaacttaa tatgctagtt
     6781 tttggataaa acatccttgc aaaataatta tgttagtaaa tgaatagtga gattttttat
     6841 acaagttaaa ctcgaattgg aagagcatag ttttctagtc gatacattca caattc
//
LOCUS       DASVEV010000237         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_893726, whole genome shotgun sequence.
ACCESSION   DASVEV010000237 DASVEV010000000
VERSION     DASVEV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893726"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VF222_10390"
     CDS             complement(<1..1950)
                     /locus_tag="VF222_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7142872.1"
                     /translation="MSDFINKKEESIHKIQRFIVHPLIKNNSIESRLYQNIIFNSSKN
                     KNSLIVLPTSLGKTVISALICAEILYNYKQSRILIMAPTRPLVLQHLLSFSSFLKVLD
                     DQKILVTGKISPSTRKMIWDNKTIKLIFATPEVIRNDLNESRLDLKDFGLIVFDEAHR
                     AIKDYAYTLISKKYIQQSPNPHIVAMTASPGSDKNRIQQICNNLYIEHIEYRNEEDAD
                     VKPYVNPIELEWEFLELSEKYRQIILLFKSMLQEKLRWLIFRGLIKKNNIDYVFKRDL
                     LELAEVLQRNLKFSLNEEKGPLYLALLNQSAALSLMYCIELIESQGPFSLKTFIKRME
                     SSEGKAHSLILKDSRMKDIFKLLENVTEHPKLIYILDLCKKEKLDTSANKNTNVLLNN
                     SQSSQILIFSHYRDTATHIVEELNKTGIKSFRFVGQSTRNNDIGMNQNEQSVILDSFR
                     KKEFNVLVATSIAEEGLDIPEVKLVIFYEPVASEIRHIQRKGRTGRKSSGNVIILAAK
                     DTVDMRILFASKRRIEKMKTIFKSMKTILNPISRVSAPYNRLTELELVDLDKNLDIEQ
                     KKLNGKELKYEANERKVIEKIKTKNEIIDKVPKFQIGMITKSENNLIEKAYRHIYDFL
                     IDERNNKNSFDLSYFHEKFTIDTHII"
     gene            complement(2181..3557)
                     /locus_tag="VF222_10395"
     CDS             complement(2181..3557)
                     /locus_tag="VF222_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7142873.1"
                     /translation="MSINTKVNLYYDKGSIVISGINQIPYSLIDPRTNQLRAPALHYQ
                     DIITYLQNSGIQYENNVMDLIPIPNLKIENIQLSLRSYQKKAIENWITAGKKGCIILP
                     TGSGKTIIAIKLIEIVNSSTLIVVPTLDLMEQWTKFLSKYFQKIEIGNIGGGISNITG
                     ITVSTYDSAFIRSSFIGNKFAFIIFDELHHLAAPGYRTIAEQFISPYRLGLTATYERE
                     DNLHLDFPKLVGGIVYRLSVNELAKDKHIASFIVEKRYVKLLPEEDIEYHKNYDQYLS
                     YLRELGIRYGQIGFQKLIMISGKNQYARNALLARNKALDIALNSQSKIEELRKILIEN
                     PNIKIIIFTQHNKLVYLISNKFLIPFITHKSSKQEREDALNGFRDGRYRVIVTSKVLD
                     EGVDVPDAELGIIVSGTGSKREFIQRLGRLLRTKPNNKNAKLIEIISSGTSEINTSIR
                     RKQALKNI"
     gene            complement(3629..3892)
                     /locus_tag="VF222_10400"
     CDS             complement(3629..3892)
                     /locus_tag="VF222_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142874.1"
                     /translation="MIQSERKCKRCAISNKKPPGFLRRNGPYILMVVAMWFFVSGLYP
                     FPYFYAVGIPVDNEIMQPVLIATAILLIPFIFMIFAWRKKPTY"
BASE COUNT         1405 a          619 c          525 g         1693 t
ORIGIN      
        1 tataatatgt gtatcaattg tgaatttttc atgaaaatag gataagtcga atgaattttt
       61 attatttctc tcatctatta aaaaatcata aatatgtcta taggccttct ctattaaatt
      121 attctcactt tttgttatca ttccaatttg aaactttgga actttatcta ttatttcatt
      181 tttagtctta attttttcaa ttactttcct ttcattagct tcatatttta attcttttcc
      241 atttaatttt ttttgttcta tatctaagtt cttatcaagg tctactaatt ctaattccgt
      301 tagacgatta tatggtgctg agactcgtga aattggattt aaaattgttt tcattgattt
      361 aaaaattgtt ttcatttttt ctattcttct cttacttgca aataaaatac gcatatctac
      421 agtatctttt gctgctagaa ttattacgtt tccacttgat tttcttccag tcctaccttt
      481 tctttgtatg tgtctaattt cactagctac tggttcataa aatataacta atttaacttc
      541 aggaatatct aaaccttctt ctgctatcga tgttgcaact agtacattaa attctttttt
      601 cctaaatgaa tccaaaataa cagattgctc attctgattc attccaatgt cattatttct
      661 agtagattga ccaacaaatc gaaatgattt gattccagtt ttgttcaatt cctctacaat
      721 atgagttgca gtatctctat aatgtgagaa tatcaaaatt tgagaacttt ggctattatt
      781 taataataca ttggtgtttt tatttgcaga agtatctaat ttttcttttt tacatagatc
      841 aagaatataa atgagtttag gatgttcagt aacattttct aatagtttga aaatatcctt
      901 cattcgggaa tctttcagaa ttaaagaatg tgcttttcct tcgcttgatt ccattctttt
      961 tataaaggtt tttagagaaa aaggaccttg actttctatt agttctatac aatacatcaa
     1021 tgatagagca gcagattgat tcaacagagc tagatataaa ggcccttttt cttcatttaa
     1081 agaaaatttt aaatttcttt gtagaacctc tgcaagttct aataaatctc ttttaaatac
     1141 atagtctata ttattttttt ttattaatcc tctaaaaatt aaccatcgta atttttcttg
     1201 taacatagat ttgaataaga gtataatttg tctatatttt tcagataatt ctagaaattc
     1261 ccattctaat tctatagggt ttacatatgg tttgacatca gcatcttctt catttcgata
     1321 ttcaatatgt tcgatgtaaa gattattaca gatttgttgt attctgtttt tgtcagagcc
     1381 aggacttgca gtcatagcta caatatgagg gtttggcgat tgttgtatat attttttaga
     1441 aataagagtg taagcataat cttttatagc tctatgggct tcatcgaaca ctatcaatcc
     1501 aaaatctttc aagtctaacc gtgactcatt caaatcattt cttattactt cgggagtagc
     1561 aaaaatcaat tttattgttt tattatccca tatcattttt cgtgttgagg gagaaatttt
     1621 acctgttacc aatatttttt gatcatccaa tactttaaga aatgaagaaa aagataacaa
     1681 atgttgtaat accaatgggc gagtgggtgc cattattaag attctacttt gtttataatt
     1741 atacaaaatt tcagcacaga tcaatgcaga aataacagtt ttacctaaag aggtaggtaa
     1801 aacaattaag gaatttttat tttttgatga attaaaaata atattctgat acaatctaga
     1861 ctcaatagaa ttatttttga tcagtggatg aactatgaat ctctgtatct tatgtatgct
     1921 ttcttctttt ttattgataa agtctgacat atggcaaatg tatatattaa aataaaatat
     1981 agtgctaatt aatttaattc tttttgtttt attttataat aatgcattat taattaatat
     2041 agaatatttt taatattttt ataattataa aaaaactact aaaattatct ctatttaatc
     2101 aattataaaa caatatttct attagtaata tatgctcaga agataagact tattataata
     2161 atatttctaa agcatatatg ttatatattt tttaaagcct gttttcgtct aatacttgta
     2221 ttaatttcac ttgttcctga cgagataatt tctataagtt ttgcattttt attattcggt
     2281 ttagttctta gtaaacgtcc cagtctttga ataaattctc ttttgcttcc agtcccactg
     2341 actattattc ctaattcagc gtctggaacg tcaactcctt catctaaaac ttttgaagtt
     2401 actataaccc gatatcttcc atctctaaaa ccatttaatg catcttctct ttcttgttta
     2461 ctagatttat gagttataaa aggaattaga aatttattgg aaattaaata aactaattta
     2521 ttatgttgtg taaatataat tatttttata ttaggatttt caataagaat tttacgcaat
     2581 tcttcaattt tagattggct atttaatgca atatctaatg ctttattcct tgcaagtaat
     2641 gcatttcgag catattgatt ttttcctgat atcataatta atttttgaaa tccaatttga
     2701 ccgtatctaa ttcctagttc ccttaaataa gacaaatatt gatcgtaatt tttgtgatat
     2761 tctatatcct cttcaggtaa caacttaaca tatcttttct ctactataaa agatgcaata
     2821 tgtttatcct tggctaactc attaacagat aatcgatata ctattcctcc aactaattta
     2881 ggaaaatcta aatgtagatt atcttcgcgt tcatatgttg ccgtcaaacc taatctatat
     2941 ggagatataa attgttctgc aatggttcta tatcctggtg cagccaaatg atgcaattca
     3001 tcaaatatta taaaagcaaa tttgtttcct ataaaagatg agcgaataaa cgctgaatca
     3061 taggtggata cagtgatacc cgtgatgtta gatatccctc cacctatatt tccaatttct
     3121 attttttgaa aatattttga taaaaatttt gtccattgct ccataagatc caaggttgga
     3181 actacaatca gggtagaaga atttactatt tcgattaatt tgattgctat tatagtcttt
     3241 cctgagcctg taggtaatat tatacatcct ttctttcctg cagtaatcca attttcaatt
     3301 gctttttttt gataggagcg taatgataat tgtatatttt caattttcaa atttggaata
     3361 ggaattaaat ccataacatt attctcatat tgaataccac tattttgcaa atatgtaata
     3421 atatcttgat aatgtaatgc tggtgctcgt aattgatttg tccgcggatc tattaaagaa
     3481 tagggaattt gatttattcc agaaataaca atagaaccct tatcatagta aaggtttact
     3541 ttagtattta tagacaatta atatattata caattatctc ttaaaaagaa atcgtataca
     3601 atctatattc ttgaatttat ataataattt aatatgtagg tttttttctc catgcaaaaa
     3661 tcataaaaat aaatggaatt aagagtatgg cagtagcaat aagaacaggt tgcattattt
     3721 cattatcgac tggaattcca acagcataaa aataaggaaa tgggtataaa cctgaaacaa
     3781 aaaaccacat tgctacaacc ataagaatgt agggaccatt tcttcttaga aatcctggag
     3841 gttttttatt ggaaattgca catcttttgc attttctttc agattgaatc aaacaagaag
     3901 tacatactgt ctgattacaa tattcacaaa ttacctcacc aaatctagat ccacatatat
     3961 aacattgaat caataataat agtagatata gtcataacga catatttaat atttgttaga
     4021 tgactttatc tactcccttc aaaaaataaa gatttacaaa taattactta atttaaaata
     4081 gtaaaattta ctttaaaaac aaaaaaaaaa taaaataaaa attttgtttg aaattctttc
     4141 cttcctttta tgggatagat ctgctgtata attttccttc taatgctagc ttgtacattt
     4201 ctgcaacgta cggattcttt cctggtgttt cagctccaag tt
//
LOCUS       DASVEV010000238         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_893793, whole genome shotgun sequence.
ACCESSION   DASVEV010000238 DASVEV010000000
VERSION     DASVEV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893793"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF222_10405"
     CDS             complement(<1..219)
                     /locus_tag="VF222_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142875.1"
                     /translation="MTKFGIYNDTPKISFETHLINLHPDQRKILIELRNFVRSIGDNV
                     VEDIRPHRIVYSKTLAFRNFLDVQPAIEE"
     gene            1027..1338
                     /locus_tag="VF222_10410"
     CDS             1027..1338
                     /locus_tag="VF222_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7142876.1"
                     /translation="MTKFLVGKVSDLSDGNMTHLTAGGQEILVANVKGNYYAIGNICN
                     HAGAELHEGELNDTELTCPWHGAKWDITTGNLVWFPQNLKSAGSYPVIIENEMIYVDV
                     H"
     gene            1425..1967
                     /locus_tag="VF222_10415"
     CDS             1425..1967
                     /locus_tag="VF222_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142877.1"
                     /translation="MEILEDRDLAKYLFDKYSTNQRKWNFIISTSPVKDAFFDAIVSN
                     PDEVWQLKIDSIYKPNPSVMGTKTTTDSSTIEKKLENTSQFGYRKLESDVLKNFLRTI
                     SEEENNNSINTMDVNFRLNSLLSSLDPVKPIKGYNYLYGPFIFTEKKMTKFNNKQDEI
                     SEKLSYRMHENIRRKYSSYG"
     gene            complement(1984..2595)
                     /locus_tag="VF222_10420"
     CDS             complement(1984..2595)
                     /locus_tag="VF222_10420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX7142878.1"
                     /translation="MTDSLIKLFLKDINPKNWISCYYNNSLIYLIIMFLFYHGIGIIS
                     SLIGTLIIDNSMVGYEEPNLPLFMFPVILAGPIEESIFFGIPFYVFGNNLFVLIGGIA
                     WAMLHIFNTYPIDVNQLAYANWLFVIPSLFFSLRTWISGKGWFAILGHSIWNLLFFSL
                     SCTFQEAYCSYSFDKEYISIALLAGILLTVVYVMMKRRQNLKK"
     gene            2710..2925
                     /locus_tag="VF222_10425"
     CDS             2710..2925
                     /locus_tag="VF222_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7142879.1"
                     /translation="MKYFISISFTTSELFQVKGNEIKVSIKSKPELGKANRELIKLLS
                     NHFQVPSDKIKIIQGIKSRKKIVHILE"
     gene            3031..3450
                     /locus_tag="VF222_10430"
     CDS             3031..3450
                     /locus_tag="VF222_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7142880.1"
                     /translation="MKVKTHYLTLNTKKRIDFINITKEVRKVIKESQIREGLCLVNAM
                     HISASIFINDNESGLHYDFENWLEKLAPHDPIEHYRHNDTGEDNADAHLKRQIMGREV
                     VVAITNGNLDFGPWEQIFYGEFDGQRSKKILIKVIGE"
     gene            complement(3604..3861)
                     /locus_tag="VF222_10435"
     CDS             complement(3604..3861)
                     /locus_tag="VF222_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142881.1"
                     /translation="MLEKSNEYVKIRNLQKMMGGKLNSTQINTIFKYLERSKLIETDL
                     DGNIIWIKKKKIDDHQTLGDIINMNEQVKNLLKDETDISKK"
     gene            4434..4580
                     /locus_tag="VF222_10440"
     CDS             4434..4580
                     /locus_tag="VF222_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142882.1"
                     /translation="MNASLKVINFAMNIRKKDHNVPILLMTAYDDIASEHSILEDLHI
                     VKGD"
     gene            complement(5031..5411)
                     /locus_tag="VF222_10445"
     CDS             complement(5031..5411)
                     /locus_tag="VF222_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142883.1"
                     /translation="MFKKPIVIVDNEAELVKLYSEALQICGYEVCSFSDPILAYDHIR
                     DNPDKYSLIITNYTMPQMDGLFLATKLAEINKKLNMIIMMSDHDDIKCNYKFNIIKKP
                     VSISKLIKLVSDSISISISSNNKI"
BASE COUNT         2215 a          809 c          742 g         2161 t
ORIGIN      
        1 ttcttctata gcgggttgta catctaggaa attacgaaat gctaatgttt tagaatagac
       61 tattctatga ggtcttatat cttcaacaac attatcgcct attgatctca caaaatttct
      121 taattctatt agaattttcc tttgatccgg atgtaaattt ataagatgag tctcaaatga
      181 aattttaggt gtatcattat aaataccaaa ttttgtcata atctatttaa cacttccttt
      241 tatttgaaat attctaccta tgtctaaaaa caataataat aatattatca atattaataa
      301 tgtaaaacac aaaaaatgaa aatttctcaa ataatttagt attgaattct agaatgatta
      361 ctcatcccta acaaatttta atatataaat tcatcctgtg acgagaaatg gttgataaat
      421 attttacctt ataaaattat ctaagatatt acaacaagga aattatatct cggttaaagt
      481 taaaagtttt taatacaagc aataaaaatt cagtaatttt gctgccaaaa aatatttacg
      541 cctagaaatt agatggactg ttcctaaaaa tctacaatat aaaactgtat gtataaataa
      601 acaatatgta cattttataa taacatataa tattcaatta attttctcca aaatacaaaa
      661 atacactgaa tagataacat gtgaaattaa actatttaaa tatacatgct actttagcat
      721 tttaacatta tttaacaata tttttgatct atttttagca ataaatttat atatatcagt
      781 ctctatccaa gttaaaaaaa gagtaaatat tcattctcac tactattgta tcttctcata
      841 tagctcacgg atctgggata cgggatgtcc ctgtaatatt atacacattt gtatgctgta
      901 gaatatagat gagtgttttt ttactaattt attcaagata ttgccaaaga ttacctaaaa
      961 ctaatttgaa ataatacact ataagaaatt tgttaaaata tgaggttaat acattattaa
     1021 tacaatatga caaaattctt ggttggaaaa gtatcagatc tttcagacgg taatatgaca
     1081 catctaactg caggaggaca agaaatctta gtagctaatg taaaaggaaa ttactatgct
     1141 attggtaata tttgcaatca cgcaggagct gaactacatg aaggagaatt aaatgacaca
     1201 gaattaacat gtccttggca cggagctaaa tgggatataa caactggcaa tttagtatgg
     1261 tttccacaaa acctaaaatc tgcaggatct tatcccgtaa taatagaaaa tgaaatgatt
     1321 tatgttgatg ttcactaaat tttgtctctc gattgttata caaatatctt ttgacttctt
     1381 atttttgcta ttaatttata tggaattttt taataatttc ttatatggaa atattagaag
     1441 atcgtgatct tgcaaaatac ctatttgata aatattctac aaatcaaaga aaatggaatt
     1501 ttattatctc aacttctcca gtaaaagacg ctttttttga tgccattgtt tctaatcctg
     1561 atgaagtttg gcaattaaaa attgattcta tttacaaacc taatccctct gtaatgggta
     1621 caaagactac aaccgattct tcaacaattg aaaagaaact tgagaatact tcacaatttg
     1681 gatatagaaa attggagtca gatgtactga agaattttct aagaactatt tctgaagagg
     1741 aaaataacaa ttctattaat actatggatg ttaattttcg ccttaattcc cttctttcgt
     1801 cactcgatcc agtgaaacca attaaaggat acaattatct ttatggacct tttatcttta
     1861 cagaaaaaaa aatgactaag ttcaataata aacaagatga aatatctgaa aaattatcat
     1921 accgaatgca tgaaaatatc agaagaaaat attcgtcgta cggataaaat agaattacta
     1981 tttctatttt tttaaatttt gtcttctttt catcattaca tatactacag tgagtaggat
     2041 accagcaagt aatgctatac ttatatattc cttgtcaaag ctatatgaac aatatgcttc
     2101 ctgaaaagta caacttaaac taaagaaaag aagattccat attgaatggc ccagtattgc
     2161 gaaccatcct tttccactta tccatgttct taaactaaaa aaaagcgatg gaattacgaa
     2221 taaccaattt gcatatgcca attgattaac atctatagga tatgtattga atatatgtaa
     2281 cattgcccat gctatcccac caataagtac aaagaggtta ttaccaaaaa catagaacgg
     2341 aatcccaaaa aatatcgact cttctatagg accagctaaa ataactggaa acataaaaag
     2401 tggaagattt ggttcttcat atcccaccat tgaattatct attattaatg ttcctattaa
     2461 ggaggaaatt attcctatcc cgtgataaaa taaaaacatt ataatcaaat atattaatga
     2521 gttattataa taacaagaga tccaattttt tggattaata tcttttaaaa aaagtttaat
     2581 taatgaatca gtcatttcat ctacaaaatt tcaaactacc ctccaatata aaaatacaac
     2641 aaatatcata aactccgtta aagagattaa cttttaaaac aataccaaca tagataaaac
     2701 acactgagaa tgaaatattt tattagtatt agctttacta catctgaatt atttcaagta
     2761 aaaggaaatg aaataaaggt atccatcaaa tcaaaaccag aattaggaaa ggctaatcgc
     2821 gaattaatta agttattatc taatcatttc caagttcctt ctgataaaat aaaaataatc
     2881 caaggtataa aatcaagaaa aaagattgtt catattttag agtaatctta taagtttttg
     2941 atacatgaag ctattttttg tatcagttat aatttaatct aaattatatc ctttaaataa
     3001 taaatataat catagctgta aaagtttaat atgaaagtta aaacacacta tttgactctt
     3061 aatacaaaaa aaagaataga ttttatcaat ataactaaag aagttagaaa ggtaataaaa
     3121 gagagtcaaa ttagggaagg tttatgcctt gtaaatgcta tgcatatatc tgctagtata
     3181 tttatcaatg ataacgaatc ggggctacat tacgattttg aaaattggct tgaaaaatta
     3241 gctcctcatg acccaattga acattataga cataatgata caggagaaga taatgctgat
     3301 gcacatctaa aacgtcaaat tatgggaaga gaagttgtgg tagctataac taatggaaat
     3361 ttagactttg gtccatggga gcaaattttc tatggcgaat ttgatggtca aagatcgaaa
     3421 aaaatcctta tcaaagttat tggtgaatga ttttaatttg ttattactat atagaaaata
     3481 aaaaagattg attaatattt tttctaaatt attattaaaa ctaatataat tgaaagaaat
     3541 agttgtaatt gaaagatact tgtttaaatt cataacaaaa attattaggc atatttttat
     3601 aaactatttc tttgaaatgt ctgtttcatc tttgagaaga ttttttactt gctcattcat
     3661 gttgattata tcgccaagag tctgatgatc atctattttt ttcttcttaa tccatataat
     3721 attcccgtca aggtcagttt caattaattt tgatctctcc aaatatttga aaatagtatt
     3781 aatctgtgta gagtttaatt ttcctcccat catcttttgt aaatttcgaa ttttaacata
     3841 ctcattggat ttttctaaca catctaaaaa gaaatcaata tcttcttcat ttactttatt
     3901 tataggtact ttatttagag gattgccttt tttctttttt tgttttatat aaataattta
     3961 tctctgtaat tataatactt tgctatgtaa tgctttcagc agtatattgc aaaataagtt
     4021 atatttgatt ttaagttatt tttacatatc tactaatgaa ttataatttt tattgctgta
     4081 tattttgcat aattcaatat aaatttagtt aggtatattt tcttattatt agcgatttta
     4141 cctatatatt tgatgtatct ttttaatatc tcattagtta ttgatattgt ttgctccaaa
     4201 cttataaaat tatagccaca ccatcattaa gcatcgcatc acattttccg tcattatata
     4261 gttaagtatt ggtcataata gtattgtata ctgtattttt tattttttta gttaaatttt
     4321 ttagtttttt catttcaaac aactattagt aatagatctg ttacttttga tgtccatttt
     4381 ctttttatcc tgtaaaagga gcaatttttc cttttcatgg ttggaatcaa tatttgaatg
     4441 cctctttaaa agtaatcaat ttcgcaatga atataagaaa aaaagaccac aatgtaccta
     4501 ttttactaat gacagcatat gatgatattg ctagtgaaca ttcaattctt gaagatttgc
     4561 atatagtaaa aggtgattga aagttcagat gttaaatatt aattttaaat tgataaataa
     4621 atgaaataga ttatataaaa tgtacaagtg ttggaaccca tccgtaacgc atataggttc
     4681 tcactagaac ccgactcgct ttattacatt agtttagtat atttcacata aagaaaagaa
     4741 atagattgtt gattaaaata ttgttcaaga atatggcaat aaacttgtaa gatgaagaaa
     4801 tatgataatg ataataaaaa agaattataa taatattaat actaacaatg agatagttac
     4861 tacaaaaatt aaaaggttca gtgaggctca tgtacgacat gtcctctagc ctggtttttc
     4921 agtccaaaga actccaaaca gaacctatca ataaaataga catatgagtt attaaagtta
     4981 tagcgaaatt tcttttatat aatttatgta agaaaataat tgtttgtaat ttagattttg
     5041 ttattactcg aaatggaaat tgatatacta tcactcacca atttaatcaa ttttgatatc
     5101 gaaacaggct ttttaataat gttaaattta taattacatt ttatatcatc atggtcactc
     5161 atcattatga tcatattcaa ttttttatta atttctgcca gtttggtagc caaaaataat
     5221 ccatccattt gtggcattgt ataatttgta attattaatg agtacttgtc aggattatcc
     5281 ctgatatgat catatgccaa aattggatca ctaaaactgc aaacctcata gccacatatt
     5341 tgtaaggctt cactgtacaa ctttactaac tctgcctcat tgtccactat gactatgggt
     5401 ttcttaaaca ttcaggtgag atctaacttg acaataatat ttaaggatgg tttaattctt
     5461 aatatcaatt tttactatac tattggtaac aatgtcacta tactagtaat taatgatggc
     5521 tttacccatt attgagaaga gaagaaatca tttttctcat atataatata atatctaaca
     5581 ttaataacta agaactcaat aaactagtct acactatagt cactctgata gccatattca
     5641 attgttatga acaatttctc atttttttag tcaggatctt ttctaaacaa ttacttgtaa
     5701 gacaaatgat attttataat tagtatagta gtaataataa tgatactaaa ttattttaag
     5761 gttctaattt tatttacttt aaaaacaaaa aaaaataaaa taaaaatttt gtttgaaatt
     5821 ctttccttcc ttttatggga tagatctgct gtataatttt ccttctaatg ctagcttgta
     5881 catttctgca acgtacggat tctttcctgg tgtttcagct ccaagtt
//
LOCUS       DASVEV010000239         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_508799, whole genome shotgun sequence.
ACCESSION   DASVEV010000239 DASVEV010000000
VERSION     DASVEV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508799"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF222_10450"
     CDS             complement(<1..413)
                     /locus_tag="VF222_10450"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142884.1"
                     /translation="MVETKVSREICLTEYPTGMLSENNFKLVQVEVPEPTKEREFLVR
                     NIWMSVDPYMRGRMRKEGGNSYIPSFKLGQPLEGACIGQVISSKNKQFAVGEYVLGML
                     GWREYWLSNGLDVIKVNPKLAPIQSFLGTFGMTGLT"
     gene            complement(553..1791)
                     /locus_tag="VF222_10455"
     CDS             complement(553..1791)
                     /locus_tag="VF222_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7142885.1"
                     /translation="MNIYPPVEKRFSPFEFYSYMRKNNPVAFDEQNQQWVLYRYRDIE
                     KILRDHIKFSSKFELFKVPQEYQENLNRPSLLNSDPPYHRKLRSIVDTLFVPIEISKL
                     EPRIENIANELIDHIIEKNNATMDLITDFAYPLPATVIAELLGVPAEDQDTFRQWADN
                     IVSLEITSDIDSIRKADKTVSDMDIYFSKLIEKKKKIPANDLITHIIRAKVDGHFLSE
                     KEILSFCGLLLNAGHVTTVNLIGNLVFSLLENPQELKRLQENQNSLIKPAIEETLRYR
                     SPVQLLIRTANADVILSEGRKEGERQEIKKGQELVLYLGSANHDESVFTDPERFDITR
                     KNLRHLGFGNGIHFCLGAPLARLEGQIALRIILEKFKDLQFNFDYTDKQDLYTKVTPF
                     KSSFLLGLSHLPLRFQSKVN"
     gene            2841..>3170
                     /locus_tag="VF222_10460"
     CDS             2841..>3170
                     /locus_tag="VF222_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7142886.1"
                     /translation="MSLNKDNSYKYVGLPSEIPIGKSKSISIKDAKSKKNINIAIFNI
                     SGRFYAISDSCAHEGGPLSKGFLNSDIVTCPWHGWKYSVKNGKSSHEGGDSVNSYKVK
                     VVKGKLYV"
BASE COUNT         1133 a          440 c          430 g         1167 t
ORIGIN      
        1 gtaagacctg tcataccaaa tgttcctaaa aatgactgaa ttggagctaa ctttggattg
       61 accttaataa catctaaacc atttgataac caatattcac gccaaccaag cattcctaag
      121 acatattcac ctacagcaaa ttgtttattt tttgatgata taacttgacc tatacaggct
      181 ccttctaaag gttgacctaa tttgaaagaa ggtatatagc tattacctcc ttctttcctc
      241 attctacctc gcatatatgg atctaccgac atccagatgt ttcttactaa aaattctctt
      301 tcttttgttg gctctggaac ctcaacttgt acaagtttaa aattattttc tgaaagcatt
      361 cctgttgggt attcagttag acaaatttct cgactaacct ttgtttcaac catatattac
      421 cgtgtataaa taagattaaa tgaatttatc tatatctata actttattat gattgtaaag
      481 ggatttttta ttgattgttc catactgtat gatatgcaag atattcaaag taaataaata
      541 tgtccaaatt tattaattaa ctttggattg gaaacgtaaa ggtaaatggg atagtcctag
      601 taaaaaagaa cttttgaatg gagtaacttt tgtatataaa tcttgtttat cagtataatc
      661 aaagttaaat tgaagatctt taaatttttc aagaattatt cttaatgcta tttgtccttc
      721 aagtctagcc aaaggagctc ctaaacagaa atgaattcca tttccaaaac ctaaatgacg
      781 caagttttta cgagtaatat caaatctttc aggatctgta aatactgatt catcatggtt
      841 ggcagaaccc aggtataata caagttcttg accttttttt atttcttgtc tttctccttc
      901 ttttcttcct tcagataaaa ttacatcagc atttgcagta cggattaaga gttgtactgg
      961 tgatcgataa cggagagtct cttcaatagc aggcttaatt agtgaatttt gattttcttg
     1021 taatcttttt aattcttgag gattttctag tagagagaaa accaaatttc ctatcaaatt
     1081 aactgtagta acatgtcctg catttaaaag aagaccacaa aaactaagta tttctttttc
     1141 agataaaaaa tgtccatcta ctttagctct aatgatatga gtaataagat catttgctgg
     1201 tattttcttt tttttctcta ttaatttact aaaataaatg tccatatcag atacagtttt
     1261 gtctgccttt ctaatggaat caatatcact agtaatttca agacttacta tattatctgc
     1321 ccattgacga aaagtatcct gatcttcagc tggtactccc aataattctg caattacagt
     1381 tgctggtaat ggataagcaa aatctgttat taaatccatt gtagcattat ttttttcaat
     1441 gatatgatct atcaactcat tggcaatatt ttctatacgt ggttctaatt tggatatttc
     1501 tataggaacg aataaggtat caacaatact acgtagtttt ctatgatatg gaggatcaga
     1561 atttaaaaga cttggacgat taagattttc ttgatattct tgtggtactt taaatagttc
     1621 aaatttagaa gaaaatttta tatggtctct taatattttt tcaatatccc tataacggta
     1681 taatacccat tgttgatttt gttcatcaaa agcaacagga ttattttttc tcatatacga
     1741 ataaaattca aaaggtgaaa atcttttttc tactggagga tatatattca tgttttctag
     1801 ctagaaaata ggaaatttat atgtaccttt ccttaatgac gaaaaaatat atttgttaat
     1861 tgtcatcaaa gttatttatt tttaatcaaa agaattaatg atataaaagt actattagat
     1921 tcaaaaatgg ttagaaaatc tattattaca taaaattatt atttgaacat cctttgatga
     1981 atcgttatat atatgaaaaa tataataatt attttgctgt tagaagattt tggtactaat
     2041 atattgtgaa ttttcttaat tgtatttgat aactttttgg tattgtctca gactaatctt
     2101 tttattaaaa tctttcattg caattatgtg aactaatata ttcgtacaga taagtagcga
     2161 gatattctta gtagtagttt tttatattaa taaaaaatta taaaaatgaa cgatataaaa
     2221 tgataatagt attatatata ataacttgaa tattttatca atagtaacat ataatcttat
     2281 tatattttta cttataaaag atcaaattat tacacttatc cttcaaaaaa tctgtaggaa
     2341 tgattcttat tatagatata attatgttta ctaattctaa gtagtgacta attataatag
     2401 caaaaaaaag taatataata ttttttgctg atatgttagt tataggagta atcactgttg
     2461 caacatcttc ctcattatta tatgaggtga aggtacaaac taaagtatca gattatataa
     2521 tggacctgtt gaatcaaaag ctctgttaaa caacaacact gttaaacaac aacaatgatt
     2581 attacaaatc aaaataccaa ttttctgaat atgacattta ttacaattca ggatatctcc
     2641 ttgaatctaa ataaaatgat tacaaggcaa aatctaggta gctttaatca gaaatttaat
     2701 tgtacaaatt cgctttagaa aaacaagaat tattagatct aaaatctttc ttactgcttc
     2761 actcctttga gaagataatc tctaaagtgt agaatgcaaa ggtaaaaccc ttaataataa
     2821 aataaagaag aatgaacaag ttgtcattaa acaaagataa ttcttataaa tatgttggat
     2881 taccttcaga aatacctata ggtaaatcca aatctatttc tataaaagat gctaaaagta
     2941 aaaaaaatat caatatagct atatttaata tatctggcag attttatgct atatctgatt
     3001 cttgcgcaca tgaaggtgga cctcttagta aaggctttct aaacagcgat attgtaacat
     3061 gtccatggca tggatggaaa tattctgtaa aaaatggcaa atcctctcat gagggaggag
     3121 atagtgtaaa ttcctataaa gtaaaggtag ttaaaggtaa attatatgta
//
LOCUS       DASVEV010000240         4690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_509190, whole genome shotgun sequence.
ACCESSION   DASVEV010000240 DASVEV010000000
VERSION     DASVEV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509190"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF222_10465"
     CDS             complement(<1..184)
                     /locus_tag="VF222_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HEX7142887.1"
                     /translation="MEINKNEFNVSSLLLDEIDKSILVHLGKNARISSYDIAKHLNDL
                     GYDITDRTIRNRLKRLE"
     gene            322..648
                     /locus_tag="VF222_10470"
     CDS             322..648
                     /locus_tag="VF222_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142888.1"
                     /translation="MNQLKNIDFFELDRVLEDVAKTYAAPSATIWFKVNNNSKPTQEE
                     YHAKVVEFIKKFEDLIKSNFPSENEHSDYLREYISKSIRYGISSINAGNNKEVERRYR
                     YYVDYN"
     gene            671..1276
                     /locus_tag="VF222_10475"
     CDS             671..1276
                     /locus_tag="VF222_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142889.1"
                     /translation="MALSLYKLAFPGIVALLAVGTAGFLLAFSYYPEKHVNVDIDGKC
                     YELLDVSNEKHKILRAEDEINVKQMQLNMIEHKDTSLPIVFSGFKKDLDDFINQYDIR
                     NILSVQKVSDNPTFDKFIIKATLAKNELQQIVNDLTLSDFYPTLSVKGSVGLGPNKYI
                     SSEEGKVISTESKDFMQNGIKNIVESNIGVGGIKLAECRNI"
     gene            complement(1524..2216)
                     /locus_tag="VF222_10480"
     CDS             complement(1524..2216)
                     /locus_tag="VF222_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7142890.1"
                     /translation="MQKLQLLLKDKLDKNTKIFFAELVGTFIVIVFATGSVVLDAKMN
                     GALGLPFIAIAPAIAVSIGIYLFGKISMAHFNPAVTVGFLITNHLPRKQKLFLTYLGA
                     ELIGALLASICIFSVLGNEVNLGANAPNYTNFPVYVIVGIEILASALLMVVILIVVYT
                     KGAKKFGGIAIGAIVGLDIFLFGLISGASMNPARSLAPAVVSGYYIDLWLYLTAPFIG
                     TSIVALLLRNKF"
     gene            complement(2185..2640)
                     /locus_tag="VF222_10485"
     CDS             complement(2185..2640)
                     /locus_tag="VF222_10485"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7142891.1"
                     /translation="MKFFEKIKISSSNKDSKTILFVCVENAGRSQMAEGFFKKYAPNG
                     FKTLSAGTKPVSQINPIVVQAMKEVGIDISKQKSKELTDEMIRESVNVINMGCMDKNF
                     CPTIWLPKVIEWDMEDPKGQSIEKVREIRDEIEQRVKELVAEITITTER"
     gene            complement(2653..3432)
                     /gene="arsM"
                     /locus_tag="VF222_10490"
     CDS             complement(2653..3432)
                     /gene="arsM"
                     /locus_tag="VF222_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX7142892.1"
                     /translation="MQKEILKEKIKENYAKIALKGNLDSCCGPQEVCDTNNISKEQML
                     SIIGYNNNELKSIPAESILGLGCGAPLNFVNLKRGETVVDLGSGAGIDAFLASKLVGK
                     EGKVIGIDMTDEMLDKARKALVVNNYTNVEFRTGDIEEKIPVDDNSVNVVISNCVINL
                     TSNKTNTFKEIYRILKKDKDARMIISDLITTKEISKDNVKTNDWCSCIDGALTKENYL
                     QSIKDAGFQNIEILNEKIYMDENHFSDGRKIVSIIVKAITN"
     gene            3493..3873
                     /locus_tag="VF222_10495"
     CDS             3493..3873
                     /locus_tag="VF222_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7142893.1"
                     /translation="MKETKGQKVKNLPGYDNCSFEGYDSEKLMLQTAKLRDLITKRGT
                     FEMLIPLCCTTKPVRYKQFRELMRGISSKTLARRLKELEKDGILERQSYNEIPPRVEY
                     KLTKKGQELVESILNLLQWMIKWS"
BASE COUNT         1690 a          700 c          636 g         1664 t
ORIGIN      
        1 tttctaatct ttttaatcta tttcttatag ttctatcggt tatatcataa cctaaatcat
       61 taaggtgttt agcaatatca tatgatgaaa tacgtgcatt ttttcctaaa tgtactagaa
      121 tagatttatc tatttcatca agcaataagc tagatacgtt aaattcattt ttatttattt
      181 ccatttagga aaacaatcat tataattctt acacataatt atcaaagtct tagataaaac
      241 aagtatctat tttccaaaat ggaaaataaa ttcaaaaaat tcttccggag aggatattat
      301 tacaaatgtc gtaatatagt tatgaatcaa ttaaaaaata tagatttctt tgaactagat
      361 agagtattag aagatgttgc aaaaacatat gctgcaccat cagctactat atggtttaaa
      421 gtaaataata atagcaaacc cacacaagaa gaatatcatg caaaggttgt tgaatttata
      481 aagaaatttg aagatttgat taaatctaat tttccatcag aaaatgaaca ttctgattac
      541 ttgagagagt atatttccaa aagtataaga tatggaattt cttcaattaa cgctggaaat
      601 aacaaagaag tagaaagaag atatcgatat tatgtagatt acaattaaaa ggtaaggatg
      661 aatacaagaa atggctttaa gtttatataa acttgcattt cctggcatag tcgcattatt
      721 agcagtcgga acagcaggtt ttctccttgc attcagttac tatcctgaaa aacatgtgaa
      781 tgtagatatt gatggaaaat gttatgaatt attggatgta tcaaatgaaa aacataaaat
      841 tctcagagct gaagatgaaa ttaatgtaaa acaaatgcag ttaaacatga ttgaacacaa
      901 agataccagt ttgccaatag tttttagtgg atttaagaaa gatctagatg attttataaa
      961 tcaatatgat attagaaata ttttatcggt acaaaaggtt tctgacaatc caacatttga
     1021 taaatttata ataaaagcaa cattagcaaa gaatgagctt cagcaaatag tgaatgatct
     1081 aacactaagt gacttttatc caacgttatc agtaaaagga agtgttgggt taggaccaaa
     1141 taaatacatt agtagcgagg aaggaaaagt aatttcaaca gaatctaaag actttatgca
     1201 aaatggcatt aaaaatattg ttgaatcaaa tataggagta ggaggaataa aattagctga
     1261 atgtcgaaat atttagttga agttagtaat catctattct ttttgctgtc atacttatac
     1321 cgtacttaaa atatcgatat aggatttttc acataaatat atctgttaag gtttttgttg
     1381 ttgtgggtta tacacagaca aagattgcct tgtgctctgc acaagtggag gctagtctag
     1441 ccgagtatga ttatgttaga catattagaa gagttaaata attgtaagaa ttaaaagttt
     1501 tactttttat tataaaaata agattaaaat ttattcctta acagtaatgc tactattgaa
     1561 gttccaataa atggtgcagt aagataaagc cataaatcaa tataataacc agatacaaca
     1621 gcaggtgcca aagaacgagc aggattcata gaagcaccag aaatcagtcc aaacaaaaag
     1681 atatccaaac ccactatagc accaattgct attccgccaa atttctttgc tcctttcgta
     1741 tatactacta tcagtattac caccataagt aaggcagatg ccaatatttc aattccaaca
     1801 ataacataaa caggaaaatt agtataattt ggagcattag cacccaaatt cacttcatta
     1861 cccaggacag aaaaaatgca aatacttgct aacaaagcac caattaattc tgctccaaga
     1921 taagtaagaa ataatttttg ttttcttggc aaatgattcg taattaaaaa tcctactgtg
     1981 actgcagggt taaaatgtgc catagatatt ttaccaaaaa gataaattcc aatagaaaca
     2041 gcaatagcag gagcaattgc gataaaagga agtcctaatg ctccattcat ttttgcatcc
     2101 aatacaacag aaccggtagc aaatactatt actataaaag ttcctacaag ttctgcaaaa
     2161 aagatttttg tgtttttgtc tagtttatct ttcagtagta attgtaattt ctgcaacaag
     2221 ttccttgact ctttgttcta tctcatctct aatctctctc actttttcaa tcgattgtcc
     2281 ttttggatct tccatatccc actctataac ctttggtagc catattgtag gacaaaagtt
     2341 tttgtccata caacccatat ttatgacatt aacactttct cgaatcattt catcagttaa
     2401 ctctttagat ttttgtttac tgatatctat tccaacctct ttcattgctt gcacaactat
     2461 tggattaatt tgtgatacag gttttgttcc tgcactcaag gttttaaatc cattaggagc
     2521 atatttttta aaaaatcctt ctgccatttg gctccttcct gcattttcaa cgcatacaaa
     2581 gagaattgtt ttagaatctt tgttgctact acttatttta attttttcaa aaaatttcat
     2641 gattacaatc tcctaatttg ttattgcttt aactattata ctaacgattt ttcttccatc
     2701 actaaaatga ttttcatcca tgtatatttt ttcatttaat atctcaatgt tttggaatcc
     2761 tgcatcttta atgctttgta aataattttc ttttgttaat gcaccatcta tacaactaca
     2821 ccaatcatta gtctttacat tatctttact aatttcttta gttgttatta gatcagaaat
     2881 aatcattctt gcatctttgt cttttttcaa gatacgatag atttctttaa aagtatttgt
     2941 cttattagag gtcaaattaa taacacaatt acttattacc acatttactg aattatcatc
     3001 aacagggatt ttctcttcta tatctcctgt tctaaattct acatttgtat aattattaac
     3061 aactaaggct tttcttgctt tgtctagcat ttcatctgtc atatcaattc ctataacttt
     3121 tccttcttta cctaccagtt tagatgccaa gaatgcgtca attccagcac cagaacccaa
     3181 gtcaactaca gtttcacctc ttttcaaatt tacaaagttt aatggtgcac cacatcccaa
     3241 tccaagaatt gactccgcag ggattgattt taattcatta ttgttatatc caataataga
     3301 caacatttgt tctttagaaa tgttatttgt atcacaaact tcctgtggac cacagcaaga
     3361 atcaagatta ccttttaaag caattttagc ataattctct tttatttttt ctttgagtat
     3421 ttctttttgc ataattatgt ttactatgga aactatttaa gttgtcaagg agactaaagt
     3481 attcaaacag tcatgaaaga aacgaaggga caaaaagtaa agaatttacc aggatatgat
     3541 aattgcagtt ttgaaggtta tgattctgaa aaattaatgt tacagacagc aaaactacga
     3601 gatttgataa caaaaagggg aacatttgaa atgcttattc cgttatgttg tacaacaaag
     3661 cctgttaggt ataaacaatt tagagaatta atgagaggaa ttagtagtaa aactcttgct
     3721 cgtagattaa aggaacttga gaaagatgga atattagaaa gacaatcata taatgagata
     3781 cctccaagag tagaatataa attgaccaaa aaaggtcaag aactagtcga atccatttta
     3841 aatttacttc aatggatgat aaaatggtct tagataatat aacaattaaa atcctccatt
     3901 ctcttatgca tttgaaattt gttttatttt tcaattagat aaatttgtct ttgttataat
     3961 actttttcac tattgttatt attatttcat ttaagatatc catagtaaaa gtacaatttc
     4021 attttttata cactatttct aaattagtat tgtttccata tgaatatctt tttttttaca
     4081 aatagaacaa tgattaatat tgaatttgtt tactaaaata gtagcaaacc aataacatga
     4141 ttcacagaat gcaaatttac aattgtgttt gtcataataa tttttgttta tactaataat
     4201 ttgtttatta gacaatttta atatcaactc ctctgttcta ttatatggaa ctgaactacg
     4261 ttaatctatt cactatactc aatatagact tatataaaaa taggtctaac aataatttca
     4321 atatttaaac cttttataga taaatcaaga tacaaacaga aacttagtta taaattgtat
     4381 aaacatcgaa ataacacttt ggttccggtc ctactctggc cgtcttactc gttgagtcac
     4441 atatttaaca aatttaaggt atatatctta gtattagaaa cagagtatat tgttatagaa
     4501 atttcaataa attatgctaa atgttgtaaa gtttatatat ccatataatg atgaatatct
     4561 tgcaatcatt atttgaatga gtataggatc gttattacac ggttgactgt ttgaatattg
     4621 aaatgacaaa cacaagtata gttcagcact aatttaattt gttattacac aaatatgtga
     4681 ttagttgaat
//
LOCUS       DASVEV010000241         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1673399, whole genome shotgun sequence.
ACCESSION   DASVEV010000241 DASVEV010000000
VERSION     DASVEV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673399"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF222_10500"
     CDS             <1..520
                     /locus_tag="VF222_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7142894.1"
                     /translation="TPPSTNISLYMAASGKEAIKTAGRLTDGLITTSKPNNSDEAFDI
                     FNKAAREVGKDPDTLEKIAKPKISYSEDYDEAFKSCEFWRSSQIDNAFDRDINDPRIL
                     EKTAKEEVSDDELRKSILIITSIEDLIKPIEEYFKAGFTQIYTHSTSPNEIEFIKKFS
                     NKVLPYFKEKKK"
     gene            860..1846
                     /locus_tag="VF222_10505"
     CDS             860..1846
                     /locus_tag="VF222_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142895.1"
                     /translation="MLIQFKIALIVLTITTFSIACMTFDPVSAIKKMDSEVNGENNNN
                     QDQLAVITNINLENIEKKGFLKVVAFINGEDFSKKIPLDKLKESTKKVKVKFEMDKEN
                     ELVSATKPDEFFVCAYHVQSPNIKSDSSVKQNSIEYFDCNEGDIQSVTEPTEVNLFKT
                     KSQVYSKSVNYHNFYAKPKVVSFVNLDNNTKTTGDNTTSSNNLKIKSNNDNDNNNNKP
                     VKVKIVVPLDDRKDIKKLKVMAMLKGQIKYEIIEDVQKEFDKIGGYTIERIFAFDKNT
                     DIGPIQIGDRFHGCASGEGLKPNEGTECEKRLIKDFDKVHTLPVHAKISPKP"
     gene            complement(2253..>3036)
                     /locus_tag="VF222_10510"
     CDS             complement(2253..>3036)
                     /locus_tag="VF222_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142896.1"
                     /translation="TKLFECMTQNNLNESSFIDENFVQILSISAIEAMKTFKVSDKAG
                     LYAWASSRDTLRGRLQQKKNYDKSNIKNDYDGVTIDQIIKILEGNTRLTCISISDPYT
                     IKDIAITLSRELLYKRKRQFKVRPYILFVFDEAQEFVQDLSNSRGIDKECSIEVETLL
                     RQGRKYGLGGCIATQRIAYLNTSALQQLHTYFVGTLPRPYDRNVVSNTFNIDNQILEK
                     TLEFAPGEWLLSSYIATGIENVPIFIRAENSEKKIESFIEKQ"
BASE COUNT         1142 a          429 c          421 g         1044 t
ORIGIN      
        1 tacacctcct tctacaaata tttctcttta tatggcagct tcgggaaaag aagctattaa
       61 aactgctggc agattaacgg atggattgat tacaacttca aaaccaaata attctgatga
      121 ggcatttgat atatttaata aggcagcaag agaagtagga aaagatcctg atacactgga
      181 aaaaatagct aaaccaaaaa tttcgtattc tgaagattac gatgaggctt ttaaatcgtg
      241 tgaattttgg agaagctcac aaatagataa tgcttttgat agagatataa atgatccacg
      301 aatattagaa aaaacagcaa aggaagaagt atcagatgat gaattaagaa aatcgatact
      361 gataattaca tcaatagaag atttaattaa accaatagaa gaatatttta aagctggctt
      421 tactcaaata tacacacaca gtacaagtcc caatgaaatt gaatttatta aaaaattttc
      481 aaataaagta ttaccatatt ttaaagaaaa aaagaaataa ataatttata tttaatgttg
      541 gattagcatt ttttaatatt ttgactgatc ttatttttaa atcaatatta attacatgat
      601 tattttattt gatgacttct tcgaatatca aataatttta ccacttttat taatttcatt
      661 taacaaaaaa ataatgtaaa aagatagtag attttatcta gtaacgaata ttaaatttta
      721 aaaaaagtct aatagtgatt aaaattttca gctacttcaa ataaaagaga gttaagaaaa
      781 tgaatataac aatttcaaat aatattataa tgctttaatc tttcccaaat ttaattagta
      841 tagcaagaga gattttctta tgttaattca gttcaaaatt gctctcattg ttctgacaat
      901 tacaaccttt agtattgcat gtatgacatt tgatcctgtg tcggcaataa aaaagatgga
      961 tagcgaagtg aatggagaga acaataacaa tcaagatcaa ctagcagtta ttacaaatat
     1021 taatctagaa aatatagaaa agaaaggttt tcttaaagtt gtagctttta taaatggtga
     1081 agatttctca aaaaagattc cattagataa actaaaagaa tcaacaaaaa aagtgaaagt
     1141 taaatttgag atggacaaag aaaatgaact agtcagtgct actaagccag atgaattctt
     1201 tgtttgtgct tatcatgtcc aatcaccgaa tataaaatcc gattcttctg tgaagcaaaa
     1261 ttctatagaa tattttgatt gtaatgaagg tgatatccaa tcagtaacag aaccaacaga
     1321 agtaaattta ttcaaaacaa aatcacaagt atacagtaaa agcgttaact atcataattt
     1381 ctatgcaaaa cccaaagtag tatcctttgt taatttagac aacaatacaa agacaactgg
     1441 agataatact acaagctcta ataaccttaa aataaaatct aataatgata atgataataa
     1501 taataataaa ccagtaaaag tcaagatagt agttccatta gatgatagaa aagatataaa
     1561 aaaactgaag gttatggcta tgctcaaagg tcagattaaa tatgaaatca tagaggatgt
     1621 tcaaaaagaa tttgataaaa taggtggtta cacaattgaa agaatattcg cttttgataa
     1681 aaatacagac ataggtccaa tacaaattgg tgacagattc catggatgtg cttcaggaga
     1741 aggtctaaaa ccaaatgagg gtactgaatg tgaaaaaagg ttaataaagg attttgacaa
     1801 agttcataca ttaccggttc atgcgaaaat ctccccgaaa ccttaaccat tatgttacta
     1861 attctattaa tttttttaaa ttaaacagta atggtgaata agatatcttc tttggattat
     1921 taataatcta agcttgttag gataatattc tagtaattaa aacaatttaa ctttttttat
     1981 ttaactattc ctatcatcat tgctaaaatt actaaagata tcatagtaga gaaaatcata
     2041 atagagttta ttgtaatatt ttttcctgat attttcttag caagttattg tggttacatc
     2101 gttcttacta ctctctcgat catttatttt actactactg agttcaatca acaacaacta
     2161 attttttttt accgaagaag ttctaatttc cttccaatat gaattgcttt agaaaattaa
     2221 gtttgatgag ttatagcagc tttgtccatt aattactgtt tttctataaa gctttctatt
     2281 ttcttttctg agttttctgc tctaataaaa ataggaacgt tctcaatacc tgtagcaatg
     2341 tagctagata acagccattc tcctggtgca aattccaacg ttttttctaa tatttgatta
     2401 tctatgttaa atgtattact cacaacgttt ctatcataag gtcttggcaa tgttcccaca
     2461 aaatatgtat gcaattgttg caatgcacta gtattaagat aggcgattct ttgtgttgca
     2521 atgcatcctc ctaaaccata tttcctacct tgcctaagta atgtttccac ttctatacta
     2581 cattctttat ctattcctct tgaatttgat aagtcttgta caaattcttg tgcctcatca
     2641 aatacaaaaa gaatgtatgg ccgaacttta aattgccttt ttcttttata gagaagctca
     2701 cgactaagag ttattgcaat gtccttgatt gtatatggat cagatattga aatacaagtt
     2761 aatcgcgtat ttccttcaag aattttaatt atttgatcta tagttacacc atcatagtca
     2821 tttttgatat tactcttatc ataatttttt ttctgttgta aacgacctct caaggtatcc
     2881 ctacttgaag cccacgcata taatcctgct ttatcgctaa ctttgaaggt tttcattgct
     2941 tctattgcag atatacttaa aatttgtaca aaattttcat caataaaact cgattcatta
     3001 agattatttt gggtcatgca ttcaaaaagt tttgta
//
LOCUS       DASVEV010000242         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1673417, whole genome shotgun sequence.
ACCESSION   DASVEV010000242 DASVEV010000000
VERSION     DASVEV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673417"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..663
                     /locus_tag="VF222_10515"
     CDS             448..663
                     /locus_tag="VF222_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7142897.1"
                     /translation="MTHVAKIIEIVGSSDKSWDDAAQVALAEATKTIHGITGIELTDM
                     TARVDPNTGKITEYHSTVKIAFGVEHS"
     gene            complement(799..1077)
                     /locus_tag="VF222_10520"
     CDS             complement(799..1077)
                     /locus_tag="VF222_10520"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX7142898.1"
                     /translation="MSNDYNSHATANEQNSLRGRENEISVKDYPSVIELANILKGLDY
                     PAAKNTIVNSVISDNTDKNIAELLGQIEDKVYNNASEVVSATGLVKRE"
     gene            complement(1208..1396)
                     /locus_tag="VF222_10525"
     CDS             complement(1208..1396)
                     /locus_tag="VF222_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142899.1"
                     /translation="MNATAKCKKCGRDFKIQGVAFAGEDYLKTAHSTGSATEERCSHC
                     SYIATYDVKELVWDDNNL"
     gene            complement(1684..2046)
                     /locus_tag="VF222_10530"
     CDS             complement(1684..2046)
                     /locus_tag="VF222_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142900.1"
                     /translation="MTNYSTEIIECLASWTQELQNCKGDPKCIEQCGNELRNRLDTIF
                     PPSTTLEFDNDKISYIFSTIFFLSNRLNKALIGVAETDKAINNLVSEERTTIDKDIDN
                     QRGQEFQNSLKKILSDYY"
BASE COUNT          746 a          364 c          331 g          832 t
ORIGIN      
        1 tgctgaccaa ggaataggcc aagaccaaga cagcgaacag aattcacaat gtgtatctgg
       61 tgaagatgca actgtttctt gcaataatgt aagtttccaa aatcaagtaa atagcgggaa
      121 gaatgcgtta gcacaacaag actaacatcc aattttttta ttggtccttg aaagatttaa
      181 taccacatta atttggaaac aaatctaata tatcaaaatt tttaatctcc taattaagca
      241 aactacgtga ttcatcctaa ccatctcctg tgatatcttg atctgtctta atttcttaat
      301 atctactaaa cagacttttg atctgacttt cctagttgtt cattttttag aatattccaa
      361 cttttattaa caaaataaag gtcaatttac atacatgaaa ccatgctact agattaccta
      421 tttaatatat aatctataat ttgaagcatg acacatgtag caaaaataat agaaatagta
      481 ggttcatctg acaagagctg ggatgatgct gctcaggtag cattagcgga agcgacaaaa
      541 acaatccacg gtataactgg aattgagtta accgatatga cagctagggt ggatccaaat
      601 accggaaaaa taactgaata tcattctaca gtaaagatag ccttcggagt ggaacattcc
      661 taaggattag cagataagga acttactatc catttttcca taaattggca tgaatttttt
      721 gccgtgatac gccttgggta gagagaatat gtttaccatt ggatagttat aaacatgaaa
      781 aacaaaagcc tttccctatt attctctttt aactaatcct gttgcactaa caacttctga
      841 tgcattatta tatactttat cttctatttg ccctaataat tcagctatat tcttatctgt
      901 attatcactt ataacagaat ttactattgt atttttggct gccggataat caagaccttt
      961 taagatattc gctaactcga ttaccgatgg ataatcttta acactgattt cattttcccg
     1021 tccccgaagg ctattttgct catttgctgt tgcatgcgaa ttatagtcat tactcataac
     1081 taattgtatc ttacatagta ttaatacagt gattctatgt gggtaatttt taaaggcgtt
     1141 tttaattatt tattatgaat gttggcctaa taacatagtt gaacaattgc ttgatgaagt
     1201 agatatatta caaattatta tcatcccaaa ccaattcttt tacatcatat gtagcaatat
     1261 aactacaatg tgagcatctt tcttccgttg cagatcctgt agagtgagca gttttaagat
     1321 aatcctctcc tgcaaatgct actccttgta ttttaaaatc tctcccgcat tttttacatt
     1381 ttgctgttgc attcatatga ttaaaaaata gtatagaaaa aacattatat aattaattaa
     1441 tcttaaagtg ctaatttttg ctaatattta attttttgtt attttctgtc tgactttact
     1501 tatacatatc gtgaaaatat atagatgaaa ttctcataat tccatctata tattttttgc
     1561 tttggcagta tatcagagaa gctacgaagc tatattaaaa attttttgtt agcatataca
     1621 acgtgattgt aaattatatg ataaatgtaa tataggtata ttcgtggtat tactatatga
     1681 tattcaataa tagtctgaaa gtatcttttt taaactattt tgaaattcct gtccacgttg
     1741 attatcgata tccttatcta tagtcgtccg ctcttctgat accaagttat taatggcctt
     1801 gtctgtttcg gccactccaa ttaatgcttt atttagccta ttagataaaa aaaatatcgt
     1861 agaaaaaata taactgattt tatcattatc aaattctaat gtagtagagg gtggaaaaat
     1921 cgtatccagc ctatttctca attcgttgcc acattgttct atacatttag gatcgccttt
     1981 acagttctgt aattcttgtg tccatgatgc cagacattct attatttctg ttgagtaatt
     2041 agtcattgaa ttattatgtg aaattaagag tataattgat ttgcttatct agatccttct
     2101 tttggagatt ggtttcatcg gtcagataag gtcaaatgac aattatcatt aatgaattga
     2161 taggatagtc acaaaaataa ctgtttaagc gattcatatc ttttaatata ttctattcct
     2221 ttcggagata taacataatg aggaatatca tcatatttac tatttattaa gag
//
LOCUS       DASVEV010000243         8975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_118127, whole genome shotgun sequence.
ACCESSION   DASVEV010000243 DASVEV010000000
VERSION     DASVEV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118127"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1172..2086
                     /locus_tag="VF222_10535"
     CDS             1172..2086
                     /locus_tag="VF222_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142901.1"
                     /translation="MTKNFSYGYIIGIVIGVILLITSNNTIAAQDQNNTTTTTIAGQN
                     QSNTTTTTIAGQNQFNPVPFNEIPLVVVNYTYGQYKFGADFSLDFGGLPVIEHDISAT
                     SVPNMRIDKDDTNLYLELQCDSNDICDTSLAPESVSIYLVDDTIQDIHIAENSIPTIQ
                     LGSNNCGSLSIEDCANFDFSIPGNIILGNYKIVVEISFDEAEWIFINPLEIVDSTSPS
                     SNNVTVRCDDLAIDLITWDLLIGGTDEPTRTNIEDMLGDEYVSPALITDIIDPSLERL
                     LGEAKDQCNNLDENVERVLEEVNFAFLP"
     gene            complement(2839..3858)
                     /locus_tag="VF222_10540"
     CDS             complement(2839..3858)
                     /locus_tag="VF222_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7142902.1"
                     /translation="MNDMTKKYYQLAPDLTICRIVNGMWQIAGGHGYIDHELAIEDMM
                     RYHDAGFTSWDLADIYGPAEDFIGEFRRRLLPLKGKEELERIHALTKWVPQPGRITRS
                     IVKENIERSLRRMNVSSLDLLQFHWWDYNNPYYMDALKYLSDLRDEGRIRHIGLTNFD
                     TERIQIMIDSDIQVVSNQVQYSIIDRRPEVKMIPFCLEHNIGLLAYGSICGGLISERY
                     LGRTRPPSTTELNTLSLRKYIKMIEAWGGWNLFQELLSTLKRIAQKYNVSIANVATRY
                     ILDKSAVAGVIIGVRLGIAEHRNNNAQVFNFSLDKSDYDDIDAVCKKSNDLFGIIGDC
                     GDEYR"
     gene            complement(3855..3986)
                     /locus_tag="VF222_10545"
     CDS             complement(3855..3986)
                     /locus_tag="VF222_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142903.1"
                     /translation="MPQKLATVLKHVKEISKSLGNASTTMRQIFVLDICDINRENIE"
     gene            complement(4194..4424)
                     /locus_tag="VF222_10550"
     CDS             complement(4194..4424)
                     /locus_tag="VF222_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142904.1"
                     /translation="MNLIEYTSFIVFAIISGIPTVFENVFAQEEAEQSPSSPFPPTLD
                     DNNDDIIISEEICPPYCSPPPPIQELPQEQSP"
     gene            5046..6683
                     /locus_tag="VF222_10555"
     CDS             5046..6683
                     /locus_tag="VF222_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7142905.1"
                     /translation="MVEREGQPQKEDNIPESGLSPFRSSLYRTLWIAALFLFIGAAMY
                     DVGASWLMTSLAPNPLFVSLITTATTLPIFLFALPSGTLSDIFDRRSILLIACAYMFT
                     ISTILGMLTLVAVTTPTVLLILTFALGAGMTMIRTPIIPILTGLVSSTELPSALTLSA
                     VAGNIGRVVGFAVGGFIVAAIAPWAVFFLNSASFIGMIIVLSKLPRKSHVNKESSLPP
                     ENIIRAIRAQIRYVRYSQAAHVLIVRAGLFTLCSSALLSLLPLLAKNELGLDSTGFGL
                     LLGSFATGAIIGGIVILPKLRPKISVESLITGSIGLLAIVIFTTGYIRDFAILCIVLG
                     LGGVAYITILSKFYTLGMKSAPKWIGARVLAVYLLILNGGLAVGSVIWGIIANTFGIP
                     ITLSVASLVLGATIIASKRYSITLLDDLDFTPVSDHWSLPPDLPVDLSQNDSQVLITI
                     DYKIDPKLSEEFEQSVRELGRILKSEGMAYWELFQDTADIGHYIEIRIADTWTDHMRQ
                     HERVTKNVKVMEDRIRVLIKDGPQPIISHYIGKSALK"
     gene            7470..>8975
                     /locus_tag="VF222_10560"
     CDS             7470..>8975
                     /locus_tag="VF222_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142906.1"
                     /translation="MITGSPFKSSTNSFDNTNPILLEIPPIQAQGDGDDLQDEGGGDD
                     FAGFEEEDDNDNGEENEEDTGQFSFETEDTEDQETDEVEDTEDQETEGGNDDAKQDTG
                     QFSFELEETDQQTVDDQVDQETEQQGQQQAVDTDDIAEQIQLHGATGMNGATGMNGAT
                     GMNAEQQGQQQAVDTDDVEQQGQQQAKQLGVGNDVAQQLGQAASQIGFDKDITQQNIG
                     QQTEKQEAFSSDIEQKISQTATQIAKSSEDQGSEEPSDVQQVIKQIATQASTDNDDDV
                     NQIVDQIYTQIANNPKSDLAKSLGNLAGLYGSGNKDQVNQAIQQIGTQIAIGDNINQN
                     TLQNIVQKSYQVTNHYFKNKIKVDIDDDDDNDDVKVIKKVYKRDNSCPTQSDSIQLKG
                     KILGKGVIVLADFEPCELRDGRATLNIPTNPNLKFVVLSLDKKGNEHDGIIVTKQKIQ
                     SLGKDSGLYVVNFDDKMIGNDPITGKKKTIDDLNGLAIYNKAAKSLNFNSGN"
BASE COUNT         3026 a         1370 c         1450 g         3129 t
ORIGIN      
        1 aaaggctttc taaacagcga tattgtaaca tgtccatggc atggatggaa atattctgta
       61 aaaaatggca aatcctctca tgagggagga gatagtgtaa attcctataa agtaaaggta
      121 gttaaaggta aattatatgt acatcctttt ccatctacaa taggtaaaaa agtatagaaa
      181 ccacatacag cctactcaga attagaaagt tcgataaata attatctaat acaaatatgt
      241 actattacta attctactac tacaacttca tctaatgagt tattacatga aagaaacaat
      301 tcaaaaataa gaattctagg gatatctaca acaaaatcaa atgattcggg gcaggcaaac
      361 ggaaggcttc gagtatgaca tgtaattggg atcggtaaac ttgtttttat ttatttattt
      421 atttatttgt ttatttgttt aatcagtaac ttatttgaaa tcttactcat attatttctg
      481 aagaatatta aaaaaataac aaaaggatca gtgggatttg aacccttcta taaagtggga
      541 ccagttactt ttttgtaata atattattat tgaaaactat tgtaataata taggataaaa
      601 ataatataaa ataaaaataa ttagaagttt taattgcact ttttatttaa atcatttaca
      661 atttcacact tagtttactc tatccttcga taagtattca aaatcagata tttctaaata
      721 gtcaaatcat tcattcatgt aattctttat taaattctat aaagttattt ttcagatata
      781 cccaagtaaa aacgattaat taatcaagaa atatgagaat agttctcact tttcttctaa
      841 taatgttttg ttttgcggcg cctcaaaatt tttcataaca aaaataaatt tactaatagg
      901 agaactggaa ttgctcagat tacttcctaa tcccattaaa taattattgt ataatgatat
      961 aaaattatat ggtatttgta ttatttatta tatttggtcg ttagatctat ggaataaata
     1021 gattttaatt atttcatata aaaaagacat tttattacat aaaatttgtt atgattattt
     1081 aactttattt ggtttcaaac ttgataataa gaattttcct gatttagtaa agaccaattt
     1141 atttttatat tattattggc atggtgtatt tatgactaaa aattttagtt atggatacat
     1201 aataggtatt gtaattggtg taatattatt aataacaagt aataatacta tagctgcaca
     1261 agatcaaaac aatactacta ctactactat tgcaggacag aatcaaagta atactactac
     1321 taccactatt gcaggacaga atcaatttaa tcctgtccca tttaatgaga taccattagt
     1381 tgtagtcaat tatacttatg gtcaatacaa atttggagct gatttttctc ttgattttgg
     1441 aggtctcccg gtaatcgaac atgatattag tgctacatct gttcctaata tgcgaataga
     1501 taaagatgat actaacttat atcttgaatt gcaatgtgat agtaacgaca tatgtgacac
     1561 cagtttagct cctgagtctg taagtatcta tttagttgac gatactatcc aagatattca
     1621 tattgcagaa aattctattc caacaataca acttggaagc aacaattgtg gtagtctttc
     1681 tatagaagat tgtgcaaact ttgacttttc tattccagga aatataatcc ttggtaacta
     1741 taaaattgta gtagaaattt cttttgatga ggcagaatgg atatttataa atcctcttga
     1801 aatagtagat tcaacatctc caagtagcaa caatgtgaca gttagatgtg atgatcttgc
     1861 tatcgacctc ataacatggg atctattaat aggtggaaca gatgagccaa cgagaacaaa
     1921 tattgaagat atgttaggtg atgagtatgt atcgcctgct ttaataactg atataataga
     1981 tccatctctt gaaaggctat taggtgaggc taaagaccaa tgtaataatc ttgatgaaaa
     2041 tgtggaacgg gttcttgaag aagtaaactt tgcatttctt ccataatctt ttttttatgt
     2101 ctgtgtatta taaaataatc taattaaaat ttttttcata tttgagaaat cttaattaat
     2161 cgttttgtta ttgtcttcgt tgtctcatta atattatatg tataaagaaa tcatctattc
     2221 ctattatttt tgcaatttgt ggtgatggtc gttattctaa tatactttca attgtaaaac
     2281 tattatctgt aatatttcaa ttaagccttt ggaattatat agatgttcta atctttcgtc
     2341 tttcctatta ctatttttat ttgatagttt gtgaactttt tttctatttt atcttttaac
     2401 tttgttggca ttcgcttgga cctcctctta ttctattctc ttcataaatg tatgattcca
     2461 attggttaac gggctcggag gtgtgtcaac tgattttgac acatttttgt agttctataa
     2521 taaataccaa ttattgaaaa atttaaacta ttgatcataa attgtattgg atagcaattc
     2581 attattttaa tagtgatgaa attgatattt tgtaatataa attcagtaaa tttgtaaatt
     2641 gttcctttgt tgtatctatt gttattatta catttaactt actccaaata ttaaatatat
     2701 aataaactca gaatacaatt gatttaccaa atcaatggtt tacagtatta ttattagtct
     2761 ttgaaatttt atcctatgtt ttaatatatt gttattgagt aaataaagta aaattatcaa
     2821 gtgaaaataa tgtgataatc atctatattc atcaccgcaa tctccaatta tcccaaacaa
     2881 gtcatttgac tttttacata cagcatctat atcatcataa tctgacttat ccaaactaaa
     2941 gttgaatacc tgtgcattat tgtttctatg ctcagctatt ccaagtctaa caccgataat
     3001 aacgcctgct actgctgact tgtcaagaat atatcgcgtt gctacatttg caatgcttac
     3061 attgtacttt tgtgcaattc tctttagagt tgataaaagc tcttgaaaca aattccatcc
     3121 tccccaagcc tctatcattt taatatactt tcgtaagctt aatgtattta actcagtcgt
     3181 tgatggtggt cgtgttcttc ctagatagcg ctctgatata agccctccac aaatgcttcc
     3241 atatgcaagt agaccgatat tatgctccaa gcaaaatggt atcatcttta cttctggtct
     3301 acgatctata attgaatatt gaacttgatt tgagacaact tgtatatctg aatctatcat
     3361 gatctgtata cgttcagtat caaaatttgt taggcctata tgcctgattc ttccttcatc
     3421 acggagatca gataggtatt tgagagcatc catgtaatac ggattgttat aatcccacca
     3481 gtggaattga agtaaatcaa gagagctaac attcattctg cgaagcgatc tttctatatt
     3541 ttccttaact atagaacggg taattcttcc tggttgtggg acccattttg ttagtgcatg
     3601 aattctttca agttcttctt ttccttttag tggcaataac ctacgtctga attctccaat
     3661 aaaatcttct gctgggccat aaatatctgc tagatcccaa cttgtaaaac ccgcatcatg
     3721 atatctcatc atatcttcga ttgccaattc atggtcgata tatccatgtc cacctgcaat
     3781 ctgccacata ccgttaacta ttctacatat tgtcaaatca ggagccagtt ggtaatattt
     3841 cttagtcata tcattcattc tatgttttct ctatttatgt cacaaatatc aagtacaaaa
     3901 atttgacgca tagtagttga agcatttcct aatgacttac taatctcttt tacatgtttc
     3961 agaacagttg caagtttttg cggcattaga tatctctcct tccttcttta atattgcaaa
     4021 atacatggat atgaatcccg atataatacg tgctcggaag tgtggcaact gtttttgaca
     4081 caattttgta gttcacaatt caagaaattt tttttcttga ttgcgattgt taatgttatt
     4141 gatattctta gattcttctt gattgttata agttgatgat tatgtattgt tgtttatggt
     4201 gactgctcct gtggtaactc ttgtattgga ggaggtggac tacaatatgg tggacaaatt
     4261 tcttcactaa taattatgtc atcgttatta tcatctaatg ttggcggaaa tggtgatgac
     4321 ggactctgct ctgcctcttc ttgtgcaaac acattttcaa atactgttgg tattccggaa
     4381 attatagcaa atacaataaa agatgtatat tctattaaat tcaaaaatga aatcatacta
     4441 tctttagttt atcaaattat ttaatatctc ttttttaaat ttcaatagta agtattctat
     4501 gtttgcaatt atttttgaat taaatcttga aaatgttatt gaactctatt acaaatgagt
     4561 tctgagggct tcgaactctc attataacag ggttcggtaa cataatattt taagttttag
     4621 tcagaaaatt attaataata ttagtaaatt aacaaccatc attattatta tttttaaaag
     4681 tgattaattt gataactaca atattcatat gagagtgttt tatttattta tatttatctt
     4741 attattattt gggtatgatt caataaccaa aagctcatct aataataata gttcgattga
     4801 ttaaaaatgt aattagtatt aagtttttaa attaatcaat ttacatctta ttaaattaaa
     4861 ttcgactggt tcttttattg ttgtaggtag aggtattcaa gtattaagcc atatgaccat
     4921 tggtgataag atggcaattg aaaaagctga taaagtatta acatccagtt ccttgtattc
     4981 ttgacaatac aatgctttag actttataga aaaatgtatg actaagtgtt gcttacttat
     5041 ttcctatggt tgaaagagaa ggacaaccac aaaaagaaga taatattcca gaatccggtt
     5101 tgagtccttt tagatctagt ctgtacagga cgttgtggat tgctgccctc tttttattta
     5161 ttggtgcagc tatgtatgat gtaggggcat cgtggttaat gacttcactt gctccaaatc
     5221 ctttattcgt ttctctgatc acaacagcga caactctacc aatttttctc tttgcattac
     5281 catctgggac attatctgat atttttgacc gccgtagtat acttcttatt gcttgtgcat
     5341 acatgtttac aatatctacg atactgggga tgcttactct tgttgctgtg actaccccaa
     5401 cggtattgtt gatacttact tttgcgctag gagcaggtat gactatgatt aggactccca
     5461 ttattcctat cctgactggt ttagtatcaa gcaccgaact accttctgct ctcaccctta
     5521 gtgctgttgc tggtaacata gggcgtgtcg ttggcttcgc agttggtggt tttattgtag
     5581 cagcaattgc accttgggct gtcttttttc ttaattcagc ttcatttatt ggaatgatta
     5641 tagtcctaag taaattacca agaaagtctc atgtcaacaa agaaagctct ctgcctccag
     5701 agaatataat tcgagctatt cgagctcaga tacgttatgt acgctattct caagcagcac
     5761 atgtattaat tgtccgtgca ggtttattca cattatgtag tagtgcatta ttatctctct
     5821 tgccactctt agctaaaaat gaattaggac ttgattcaac aggatttgga ctgctattag
     5881 gatcttttgc tacgggtgca ataattggtg gaattgtaat tttgccaaaa ttgcgaccta
     5941 agatttcagt agaatctctt attacaggtt ccataggatt acttgcaatc gtaatattca
     6001 caacgggata tatacgagat tttgctattc tttgtatagt tctagggcta ggaggagtag
     6061 cttacatcac gatattatcc aaattttata cccttggaat gaaatctgcg ccaaagtgga
     6121 ttggagccag agtactagca gtttatctgc ttatactaaa tggtgggttg gcggttggaa
     6181 gtgtaatatg gggtataata gctaacacat ttggcatccc aatcaccctg tcagtagctt
     6241 cattagtctt aggtgcaact attattgcta gtaagcgtta tagtataact ttattggatg
     6301 acctcgattt tacaccagta agtgaccact ggtcactccc tcctgatctc ccagttgatc
     6361 tttcacaaaa tgacagtcaa gtcctaataa caatcgacta taagattgat cctaaattat
     6421 ctgaagagtt cgaacagagt gtacgtgagt tagggcgtat tcttaagagc gaaggaatgg
     6481 cctattggga attattccaa gacacagcag atattggtca ctatatcgaa atcagaattg
     6541 cagatacctg gactgaccac atgcgtcagc atgaacgcgt taccaagaac gttaaggtta
     6601 tggaagatag aattcgagta cttatcaaag atggcccaca accaataatc tctcattata
     6661 taggaaaatc tgctctcaaa tagttaacca gcctagtccc ctttttattg caattatttg
     6721 agttctgtac tattttcaga cctacataat ttcattattc tcttcaaatt taaagtgaga
     6781 ttattcattt cacagaattg ccagtataat taaagcctac actaatcata ttttttttat
     6841 tttagtttca tttactaatc tatgaacttc cttgtactgt gattttaatt ttgttaaaat
     6901 cttttagtaa cttttttgta tattctctta aaagtatatg ttcctaattg gttaacgggc
     6961 tcggagggct tcgagtaggg catgtaattg ggatctgctg ttaaattaat ggtaatagta
     7021 ataataatag taataataat aatattttta ccatatagtt tcggaggtgt gtgaactgtt
     7081 tttgacacaa aaattgtagt tcaagtagaa ttttaattaa gaatatttca aatagaaact
     7141 aaaaatctaa ggttatattt caaagaacat ttggaaaact atcaatagat gatagtaatg
     7201 tccaagttat ggaagtatag acaattattg ataatgataa tgaggacaat aatataaatc
     7261 cgatgccaaa taaaagaagg ataaataaat catataagat ttatctactc atacactata
     7321 aaatttttgc acaatttttt tcttattttt atacctcaac aattttttta aactatttta
     7381 ttagataatt caatataatt caacagttaa atttgaaaag accgaattta ttagtaataa
     7441 taagtttaat cagttttctt agtataattt tgattactgg ttctcctttc aaaagttcta
     7501 caaattcttt cgataatact aaccctattc ttttagaaat tcctccaatt caagcccaag
     7561 gtgatggaga tgacttgcag gatgagggtg gaggagatga ctttgctggt tttgaagaag
     7621 aggatgataa tgataatgga gaagaaaatg aggaagacac tggtcaattt agctttgaaa
     7681 cagaagacac tgaagatcaa gagactgatg aggtagaaga cactgaagat caagagactg
     7741 aaggaggaaa tgatgatgca aagcaagata ctggtcaatt tagctttgaa ttagaagaaa
     7801 cagatcagca aactgttgat gatcaagtag atcaagagac agaacaacaa ggacaacaac
     7861 aagctgttga tactgatgat atagcagagc agattcaact acatggagct acaggtatga
     7921 atggagctac aggtatgaat ggagctacag gtatgaatgc agaacaacaa ggacaacaac
     7981 aagctgttga tactgatgat gtagaacaac aaggacaaca acaagctaaa caattaggag
     8041 tgggtaatga tgtagcacag cagttaggac aagcagcttc acaaatagga tttgataaag
     8101 atataacaca acagaatata ggacaacaga cagaaaaaca agaagcattt agttccgata
     8161 tcgaacaaaa aattagtcaa acggctaccc agattgctaa atcatcagag gatcaaggaa
     8221 gcgaagagcc ttcagatgtt caacaagtaa ttaagcaaat tgcaactcaa gcctcaacag
     8281 ataatgatga tgatgtaaac caaattgttg accaaatata tacacaaata gctaataatc
     8341 caaaaagtga tctggcaaaa tcattaggta accttgctgg cttgtatggg tcaggtaata
     8401 aagaccaagt aaatcaagct atccaacaaa tagggactca aatagccatt ggagacaaca
     8461 ttaaccaaaa tactcttcaa aatatagttc agaaatcata tcaagtaacc aatcattact
     8521 ttaagaataa aataaaagta gatatcgatg acgatgatga taatgacgat gttaaagtaa
     8581 tcaaaaaagt ctacaagaga gataattcgt gtcctactca atctgattca atacaattaa
     8641 aaggaaaaat acttggaaaa ggagtcatag ttttagcaga ttttgaacca tgtgaattaa
     8701 gagatggacg tgccacttta aatattccaa ctaatcctaa tcttaaattc gttgttttat
     8761 cattagataa gaaaggaaat gagcatgatg gaataatagt cacgaaacaa aaaattcaaa
     8821 gccttggcaa agatagtggt ctatatgttg taaattttga tgataaaatg attggaaatg
     8881 atcccataac tggaaagaaa aaaacaatag atgaccttaa tggattagca atatataata
     8941 aggcagctaa atctcttaac tttaactcag gaaac
//
LOCUS       DASVEV010000244        11499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1675004, whole genome shotgun sequence.
ACCESSION   DASVEV010000244 DASVEV010000000
VERSION     DASVEV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675004"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..817
                     /locus_tag="VF222_10565"
     CDS             86..817
                     /locus_tag="VF222_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7142907.1"
                     /translation="MGKRPLVRRRGRGGKQFRAITVGKILPARYPNFKLEEQHEGKIV
                     DIIHERGRDAPLAKVEFDNNNISYIPAPEGTSVGSIIKTGIGTPATSKNILSLESIPD
                     GTLICNIEKNAGDGGKLIKAAGSSAIVFSHSSEGVTVKLPSGKFLLVNQKCRAMIGAI
                     AGAGRKEKPFLKAGNKSIYMKSRGKIYPHVRGIAQAAVYHPHGGGRHQHIGRQSSVGR
                     TTPPGRKVGNIAPRKTGRGRLKDRR"
     gene            complement(935..1870)
                     /gene="argF"
                     /locus_tag="VF222_10570"
     CDS             complement(935..1870)
                     /gene="argF"
                     /locus_tag="VF222_10570"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7142908.1"
                     /translation="MNSLKNKHILSLQDLDLKEIISILDFATNLKKEMNTGSSRKLLE
                     GRTLGMVFQKPSTRTRVSFETGMFQLGGHAIFLGTNDLQLSKGESIEDTARTLSLYLD
                     CLMARVYDHSDLEKLSKYSSIPIINGLSDLFHPCQILADLLTIREYKKKFNGLNLAWI
                     GDGFNVCNDLLIGCAKTGINITAACPSGYEPPEWIVKISKREAAKTGSKIIVTNEPIR
                     AVQEADIVVTDTFVSLGKDDEKIIRETIFIPKYQVNSELMRYAKDDAIFMHCLPAKRE
                     QEVTSDVIDGKSSVIWKEAENRLHVQKALLCLLMR"
     gene            complement(1851..3332)
                     /gene="ftsY"
                     /locus_tag="VF222_10575"
     CDS             complement(1851..3332)
                     /gene="ftsY"
                     /locus_tag="VF222_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7142909.1"
                     /translation="MFDKLKKAFSNIISDISKKEISEKDLENTLFDLEIALLESDVAQ
                     EVIDDISASLKNKLLGFSVEKDKTPEELIKFHVQKSIYEILTKSGTLDLVKEINSKKE
                     NKRGPFVVVFLGINGTGKTTTVAKVANLLHKNGLSVVLAASDTHRAGAIEQLGEHARR
                     LSLKMIHQRYGADPSAVARDAVEYGKKHRVDAILIDTAGRMQTAKNLMDEISKIVQVV
                     KPDMKIFIGDSLAGNDAIYQAKEFFAYTKFDASILTKTDADAKGGAIISITYLTSKPI
                     IYLGVGQNYDDIIPFDLDTFVESLFGSQISEEITNKYSSNIINESKNNEKLPDIQSKS
                     ISNNEPIHNIEELYQNTPSSSNLDPIISNNINNHDIAISKVELKEEEKKVDDLRNNNI
                     INLENESQVGKSEKIAENDDEKKKGFFSSLFGKRKSLVNSDKVSSTTADQKSKQNNQG
                     KPNSDKHQYNTINKNEISEKNKNKDKNEVKYLSDDDLDELLKE"
     gene            complement(3404..3844)
                     /gene="pfdA"
                     /locus_tag="VF222_10580"
     CDS             complement(3404..3844)
                     /gene="pfdA"
                     /locus_tag="VF222_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HEX7142910.1"
                     /translation="MSNKANTNKSAIEQRVNELVQHSRTLELYLNEVLEREATVTKLI
                     EEARLASYALQNIPLDKENEALMPIGIGVYLKTNITSIDKLLVNIGAGVTIEKTHEDT
                     LHYIESRIKEFEIGLNQLREQKKQITSQLEQIQHQVNSMLQQTS"
     gene            complement(3927..4952)
                     /locus_tag="VF222_10585"
     CDS             complement(3927..4952)
                     /locus_tag="VF222_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7142911.1"
                     /translation="MKIAVVGIGVAGAYLMNQLSNNHDIHVVGFERMKEEEHDAVCAW
                     ATSKNVMVDYTKKCSLDFDNYILHDGKNMSVDLGESKHVDIGLKGMVSYDKLKLIQDM
                     IKGTEIKYGKAPKKENLEADFDLIVDSTGFHRNYLPKLDYEMWVPCVQYKVKYDNNNV
                     PYDDFYLKGFPSMSGYFWYFPLGNGYAHIGAGDFTRKNHNLFVENFLKKYKCEIIKKN
                     GRPVRISPPSKSEPFYDGKKSIGVGESIGTVYPLLGEGIIPSTICADLFINNLNDFKK
                     YREQVLKYFSIYPLVFKFILMKITGTFNLVKNSFDLLRIYYHMKNNQDRYGMEVKIAE
                     LMKVATI"
     gene            5036..5233
                     /locus_tag="VF222_10590"
     CDS             5036..5233
                     /locus_tag="VF222_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142912.1"
                     /translation="MPGPARAGIAILVLGAILLGIGYYQDRGALSLYGLIMSICGFSL
                     YMFSSIYISRKSNQEKGSYSK"
     gene            complement(5221..8262)
                     /gene="leuS"
                     /locus_tag="VF222_10595"
     CDS             complement(5221..8262)
                     /gene="leuS"
                     /locus_tag="VF222_10595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7142913.1"
                     /translation="MNSREINDNSINWKELEAKWKSRWKEEHLFEADYDNTNKKKYFI
                     TVAFPYPNSPQHIGHGRTYSLADVHARYMRMKGYNVLFPMGFHYTGTPILAMSKRVIA
                     EDQDLIGTFKNLYNVPENIIKSFIEPENIAKFFHNEIKLGMQDMGYSIDWRREFTTID
                     TLYSKFITWQFKILQKKGLIVQGSHPVGWCPYDKNPVSQHDTIGDVEPEFSEYVLIKF
                     RLAENDIILPTATLRPETIFGVTNLWINPDIEYFIIDVDNNTEQWIVTKEASTKLEFL
                     NHSVKINRIIKGNELIGKFATDIIRNVNIPILPAKFVKTDTGTGIVMSVPGHAPYDFQ
                     ALIDLKKEVNLPSFDIDLSQINPIKIIEIQDANLNDNSLISCVEDAISKFKINNQSDL
                     GLEKATNEMYHLEYYQGKMLPNTGKYSGMDVQLAKEEIKNYLLATKNLAEIFYEFINK
                     PIKCRCGNECFVKLLNDQWFINYKDKEWKHRVHNYIDELSIIPDDLRQEFHNVVDWLK
                     ERACARKSGLGTKLPWDHEWIIESLSDSVIYMAYYIISKFLNEQKYSMKKSLNNIDES
                     FFDYILLGIGNVHDVAKNCNLSISLIQEIRNEFIYFYPVDSRHSGRDLVPNHLSFFIF
                     NHVAIFEKKFWPHQIVVNGSVLMEGKKMSKSLGNIIPLRTAIQEYGADTIRLAILISS
                     ELLQDADFSFDTARSIRIKLLEFYTLSQQILNSNFYNKISDSNLSLDESLYLDLSTNE
                     YPLELEDRWLISKLQRLISNVTKSMETLRLREALHNILYVFDQDMHWYKKRINAKKRG
                     NDNMVNKILILCFSIRIKLLSPFAPFISEEIWNSLGNRSSVVSSKWPSVFNEKIDPLS
                     EESDEFIKRLTLDISNIIKVTKKLPRTIYIYCSSSDKQKIYQKILNLITLTNERNFGN
                     IMKKLIQDPETSYAKKDPSFVKKTVEDILSESIESRINRLNLNSFDEISIIKDSQNLI
                     SNEIESENIPIIIYSEEEKEKFDPKLKSRFSRPYKPAIYLE"
     gene            complement(8269..11016)
                     /gene="alaS"
                     /locus_tag="VF222_10600"
     CDS             complement(8269..11016)
                     /gene="alaS"
                     /locus_tag="VF222_10600"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7142914.1"
                     /translation="MGKKEISTLFSSNPDKYYRVSLFDRLGFKRNKCISCEKFFWSLI
                     DKNICPDHEAYNFIGNPPTNKRLDYIGAWKEIEKYFQSNDHSIVRRYPVVCRWREDLY
                     FTIASIVDFQRIMNNKVIFELPSNPLVVPQMCLRFNDVENVGVSGRHYTSFCMIGQTC
                     NADAPGGYWKDHCIDLDFGLLTNVLGIKPEEITFVEDIWMGAGAFGCSLEYFVRGLEL
                     GNAVFTEFEGDENNYRIMKNKIIDMGAGLERLSWITMGTPTSYDCSFGPVIKYLENSI
                     EVQKDENFLSNYFKLVSSKFNIIEDDSTDKIKQLKKTIAQEMRIPFEHLEKIVSPYEA
                     IYTIADHTRTLLFAISDGSLPSNVGGGYNLRILLRRVLSIIERFKWNIKIEDLIDTHV
                     EYLKNMYPELEENKQEVYTILKLESDRYVSTRQRMNDIASTIRKNNKQLTVDDLIRLY
                     ESDGITPDFLKEINVITDIPSNFYVKLSELHNINQKSLYHPSIEGLEGLSPTRLLYYE
                     DSSIREFYAQIIKIKENYVILDQTSFYPRGGGQEPDKGNIGDVKVLEVIKQGDIVLHR
                     VENLSSLSEGLRIKCRLDDVRRDSITKHHTATHILNSSSRTTLGSWVWQNSAFKDVNY
                     GRLDITHHSALNKEEVERIEKTANEIVMKNLDVNIKEYDRGESEQKFSFRIYQGGVVP
                     SNKVRIVKISEFDIEACGGTHVNRTGELGLIKILKSERIQDGVVRLEFVAGQAAIDYI
                     KNQDRNISFITQSLGSSKEKLTHSFKKYLDESEKLKTKIKILLRKTSPILAKSISTNS
                     KQISSNGVKLFTTYDDELDEEFHITIGEKSINLDPFLIYISLISKGEGEGIRVIVFVG
                     ETARKYGFNAGKIAKVFSISLGGSGGGDSKFGQGGGRFKNRIEEVLHSSEIYVNETLK
                     R"
     gene            complement(11111..11452)
                     /gene="trxA"
                     /locus_tag="VF222_10605"
     CDS             complement(11111..11452)
                     /gene="trxA"
                     /locus_tag="VF222_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7142915.1"
                     /translation="MGTEHSVTQVNNASWEKEVLQSQLPVFVDFWAEWCGPCRMVGPA
                     VEQLSKTLEGKVKTVKLNVDENQEIAVKYGIHSIPSLLLFKDGKEIARTVGAAPKEAY
                     QKFIEESLTKN"
BASE COUNT         3727 a         1867 c         1575 g         4330 t
ORIGIN      
        1 taatctaata ttaatattga tttttttctt ttttaaaaca caaaagttaa aatcacctaa
       61 ttgaaacgat tactatagaa ttattttggg taaacgacca ctagtacgaa gaagaggaag
      121 aggaggaaag cagttcaggg ctattacagt aggaaagata ttacctgcta gatatcctaa
      181 ctttaagtta gaagaacaac atgaaggaaa aattgtagac ataatacatg aaagaggaag
      241 agatgctcct ttagctaaag tagaatttga taataacaat atatcttata ttccagcacc
      301 agaaggtact tctgtaggca gtataattaa aacagggatt ggtactcctg caacttcaaa
      361 aaatatttta tcattagaat caattcctga tggaacttta atttgtaata tagaaaaaaa
      421 tgcaggagat ggaggaaaat taattaaagc tgcaggatca tcagctatag tcttttcaca
      481 ttcttctgaa ggtgtaactg taaaattacc ttcaggaaaa tttttacttg ttaatcaaaa
      541 atgtcgtgcg atgataggag ctattgcagg tgctggaaga aaagaaaaac cctttttgaa
      601 agctggaaat aagtcaattt atatgaaatc cagaggtaaa atatatccac atgtaagagg
      661 aattgcacaa gcagctgtat atcatccaca tggtggagga agacatcaac atataggacg
      721 tcaatcatca gtaggtagga ctactcctcc aggaagaaaa gtaggaaata ttgctcctag
      781 aaagacagga agaggtagac taaaggatcg tagataggta gcagcactat ttttatattt
      841 atttaggaaa caaattaaat ttatatattt aagattaaag atcaagaaaa taataacttc
      901 taacaataac aactaattaa atacataata ttatttatct catcaacaaa cataatagcg
      961 ctttctgaac atgaagtcta ttttcagctt ctttccaaat gacggatgat tttccatcaa
     1021 tcacatcaga agtaacctcc tgctcgcgtt ttgcaggaag acaatgcata aatattgcat
     1081 catcttttgc atatctcata agttcagaat taacttgata tttaggtata aatatagtct
     1141 cacgaattat tttttcatca tctttcccta aagagacaaa tgtatcagtt actactatat
     1201 ccgcttcttg tactgcacgt ataggctcat ttgttactat aattttagaa ccagtttttg
     1261 ctgcttctct ttttgatatt ttgactatcc attctggagg ttcatatcct gaagggcagg
     1321 ctgcagtaat atttattcct gttttagcac aaccgattaa aagatcatta catacattaa
     1381 atccatctcc tatccaagca agatttaatc cattaaattt ttttttatat tctcgaattg
     1441 ttaaaagatc agccaagatt tgacaaggat gaaaaagatc ggataatcca ttaataatag
     1501 gtatagacga gtatttagat aatttttcta aatcgctatg atcataaact cttgccatta
     1561 aacaatccaa atataatgac aatgtccttg ctgtatcttc tattgactct ccttttgata
     1621 attgcaaatc atttgttccc agaaagatag catgaccgcc cagttgaaac atacctgttt
     1681 caaaactaac tctagtacgt gtagacggtt tttgaaaaac cattcccaat gttctccctt
     1741 caagtaattt tctagatgag cccgtgttca tttctttttt aagattagtt gcaaaatcca
     1801 gtatcgagat aatttctttt agatccaagt cttgtaagct aagaatatgt ttattcttta
     1861 aggagttcat ctaaatcatc gtctgaaaga tatttgactt catttttgtc tttatttttg
     1921 tttttctcag atatttcatt cttgtttata gtattgtatt ggtgcttgtc tgaattgggt
     1981 tttccttgat tattttgttt acttttttga tcagctgttg ttgatgaaac tttatcgcta
     2041 tttactaggg attttcgttt accaaataag gaagaaaaga agcctttttt cttttcatcg
     2101 tcgttttcag ctattttttc agatttacca acttgtgatt cattttctaa attaataata
     2161 ttattatttc ttaggtcgtc aacttttttc tcttcttctt ttagttcaac tttagatatt
     2221 gcaatatcat gattatttat gttattacta ataataggat ctaaatttga agaagaaggt
     2281 gtattttgat ataattcttc aatattatga ataggctcat tattagagat acttttggat
     2341 tggatatctg gtaacttttc attattttta ctttcattta taatattaga actatactta
     2401 tttgtaattt cttctgaaat ttgagaacca aacaatgatt ctacgaaggt atcaagatca
     2461 aatggaatta tatcatcata gttttgacct actccaagat aaataatagg tttagaagta
     2521 agatatgtaa ttgaaatgat agcacctccc tttgcatcag catctgtttt tgttaaaatt
     2581 gatgcatcaa atttagtata ggcaaagaat tcttttgctt gatatattgc atcatttcct
     2641 gccaaagaat ctcctataaa aattttcatg tctggtttaa caacctgaac aattttacta
     2701 atttcatcca tgagattttt tgcagtttgc attcttccag cagtatcaat taaaattgca
     2761 tctactcggt gcttttttcc atattcaact gcatctcttg caactgcaga tggatctgct
     2821 ccgtatcttt gatgaatcat ttttagtgat aatcttctag catgttcacc cagttgttct
     2881 atagcacctg ccctatgagt atcacttgct gctaatacaa cagacaaccc atttttatgt
     2941 agaagattag caactttggc tacagtagtg gtttttccgg ttccattaat tcctagaaaa
     3001 actactacaa atggacccct tttattttct tttttggaat taatttcctt aactaagtct
     3061 agggtccctg atttagtaag aatttcataa atagattttt gaacatggaa tttaattaat
     3121 tcttctggtg ttttatcttt ttcaacagag aaacccaata atttattttt caaactagcc
     3181 gagatatcat ctataacttc ctgtgctaca tcactttcta gcaatgcaat ctcaagatca
     3241 aatagagtat tttcaaggtc tttttcagaa atttcttttt tactgatatc tgaaataata
     3301 tttgaaaaag ctttttttaa tttatcaaac atatatactc atacacaaaa ataatttaaa
     3361 tctcaatgta ttatgttttc tttttttgct ttgattaaat attttatgaa gtttgctgca
     3421 acatcgaatt cacctgatgt tggatctgtt caagttgaga agttatttgt ttcttctgtt
     3481 ctcttaattg atttaagcca atctcaaatt ccttgattct tgattctatg tagtgtaaag
     3541 tatcttcatg tgttttttct attgtaactc cggcaccaat attaaccaat aatttatcaa
     3601 ttgaagtaat atttgttttt aaatacactc ctattccaat tggcattaat gcttcattct
     3661 ctttatctag aggaatattt tggagagcat acgatgcaag acgagcctct tcaattaatt
     3721 ttgtaactgt agcttctctt tctaatactt cattaagata aagttctaag gttctggaat
     3781 gctgaacaag ctcatttact ctttgttcaa ttgctgattt attcgtatta gctttattgc
     3841 tcatctgtat tgaattattt taagtccatt tataactgtg actcaaaagg aaaaaggtaa
     3901 agatattgga atttttcaaa taatagttaa atagtagcga ctttcatcaa ttcagcgatc
     3961 tttacttcca ttccatatcg atcttgatta tttttcatat gataatatat tctaagtaaa
     4021 tcgaaagagt tttttacaag attaaaagta cctgtaattt tcatcaaaat aaacttgaaa
     4081 actaatggat atatactaaa atatttaagg acttgttctc tatatttttt aaaatcattc
     4141 aaattattta taaagagatc cgcacaaatt gttgaaggga ttatgccttc accaagtaat
     4201 ggatagactg taccaatcga ttctccaact cctatagact ttttaccatc ataaaatggt
     4261 tctgattttg atggtggact aattcttaca ggtcttccat tttttttaat tatttcacat
     4321 ttgtatttct ttagaaaatt ttcaacaaaa agattatgat tttttcttgt aaaatcacca
     4381 gcacctatat gtgcatatcc atttcctaat ggaaaatacc agaaatagcc agacatagaa
     4441 ggaaatcctt taagataaaa atcatcatat ggaacattat tattatcata tttgacttta
     4501 tattgaacac aaggtaccca catttcgtaa tcaagtttag ggagataatt cctgtgaaaa
     4561 ccagtagaat ctacaattag atcaaagtca gcttctaaat tttccttttt tggtgctttt
     4621 ccatatttaa tttctgttcc ttttatcata tcttgaataa gttttaattt atcataacta
     4681 accatacctt ttaatccaat atcaacatgc ttgctttcac caaggtctac gctcatattt
     4741 ttaccatcat gtaagatata gttatcaaaa tctaggctac atttttttgt gtagtctacc
     4801 ataacatttt tgctcgtcgc ccaagcgcaa actgcatcat gttcttcttc tttcattctt
     4861 tcaaatccaa ctacatgaat atcatgatta ttactaagtt gattcattaa atatgctcct
     4921 gcaaccccaa ttcccaccac agcaattttc atagatacaa tattaccatc caaaaataaa
     4981 aatatattgc gaaaaaatta aacacttacc actagtggtg aaaaagaaaa aagaaatgcc
     5041 aggtcctgca agggcaggta tagctatttt ggtattagga gcgatacttc ttggtatagg
     5101 atactatcaa gatagaggag cactttcttt atatggatta ataatgtcaa tctgtgggtt
     5161 tagcttgtat atgttttcat cgatttatat ttcaagaaaa agtaatcaag aaaaaggatc
     5221 ttattctaaa taaatagcgg gtttatatgg tcttgaaaat ctagacttta attttggatc
     5281 aaatttttcc ttttcttctt ctgaataaat aattattgga atattttcag attcgatttc
     5341 attagatatt agattctgtg aatcttttat tatagagatt tcatcaaatg aattcaaatt
     5401 taatctgtta attcttgatt caatagattc agaaagaata tcttctacag tttttttaac
     5461 aaatgaagga tctttttttg cataagaagt ttcaggatct tgtattaatt ttttcattat
     5521 atttccaaaa ttcctttcat tagttaaagt aattaagttg agaattttct gataaatttt
     5581 ttgtttatca gaagaagaac aatatatata tattgtacgt ggtaattttt tagttacttt
     5641 aataatatta gagatatcca acgttaatct ttttatgaat tcatctgatt cctcagataa
     5701 tggatcaatc ttttcattga aaacactagg ccattttgaa gaaacgactg aagatcgatt
     5761 acctaaacta ttccaaattt cttcagatat aaatggagca aatggagata ataattttat
     5821 tctaatagaa aaacaaagaa ttagtatttt attcaccata ttatcattac ctcgcttttt
     5881 agcattgatt ctttttttat accaatgcat atcttgatcg aaaacgtaca aaatattatg
     5941 gagagcttca cgtaaacgta atgtttccat agatttagta acattactta ttagcctttg
     6001 taatttactt attaaccatc tatcttctaa ctccaaagga tactcatttg tagatagatc
     6061 cagataaagt gattcatcta atgataaatt tgaatctgat attttattgt aaaaattaga
     6121 attcaaaatc tgttgtgaaa gagtatagaa ttctaataat tttattctaa tagatcttgc
     6181 agtatcaaag gaaaaatctg catcctgaag aagctcagat gaaatcaata ttgctagtct
     6241 aattgtatct gcgccatatt cttgtattgc tgttcttaaa ggtattatat tacctaaaga
     6301 tttagacatc tttttacctt ccattaaaac agaaccgttg acgactatct ggtgtggcca
     6361 gaacttcttt tcaaaaatag caacatgatt aaaaataaag aaagaaagat gattaggaac
     6421 caaatctcgt ccagaatgtc gagaatcaac tggataaaaa taaataaatt catttctaat
     6481 ttcttgtatt aatgatatcg aaagattaca attttttgct acatcatgaa catttcctat
     6541 tcctaatagt atatagtcaa aaaaagactc atcgatatta ttgagacttt ttttcattga
     6601 gtatttttgt tcattaagga actttgatat tatataataa gccatgtaaa taactgaatc
     6661 agataggctt tctattatcc attcatgatc ccatggaagt ttcgttccaa gacctgattt
     6721 ccttgcacaa gctctttctt ttaaccagtc aactacattg tgaaattctt gtctaagatc
     6781 atctggaata atacttaatt catcgatata attatgtact ctatgcttcc attctttatc
     6841 tttataatta ataaaccatt gatcattcag taacttgaca aaacactcat taccacatct
     6901 acatttaatt ggcttattta tgaattcata aaagatttca gctaagtttt ttgtagcaag
     6961 caaataattt tttatttctt ctttagctaa ttgaacgtcc attccagaat attttccagt
     7021 attaggtaac atcttaccct gataatattc taaatgatac atttcatttg ttgctttttc
     7081 caggcctaaa tctgattgat tgttaatttt gaactttgat attgcatctt ctacacatga
     7141 tattaagctg ttgtcattta gattagcatc ttggatttct ataattttaa tcggattgat
     7201 ttgtgataag tcaatatcaa atgagggtaa atttacttct ttttttaagt caataagggc
     7261 ttgaaaatca tatggagcat gaccaggtac cgacattact attcctgttc ctgtatcagt
     7321 ttttacaaat tttgcaggaa gaataggaat atttacattt ctaataatgt ctgttgcaaa
     7381 tttaccaatt aattcatttc ctttgatgat tctattgatt tttacactat gattaagaaa
     7441 ttctaatttt gttgaagcct ctttagtaac aatccattgt tcagtattat tatctacatc
     7501 tattataaaa tattcaatat caggatttat ccaaagattt gtgactccaa atatagtttc
     7561 aggtcttaaa gtagcagtag gaagtattat atcattctca gctaacctaa actttattag
     7621 gacatattca ctaaattctg gttctacatc tccaatagta tcatgttgac ttacaggatt
     7681 tttatcatag ggacaccatc caacaggatg agaaccttgt acaattaacc cttttttttg
     7741 taatatttta aactgccaag taatgaattt tgaatataaa gtatcaatgg ttgtaaattc
     7801 tcgtcgccaa tcgatagaat aacccatatc ttgcattcct aattttatct cattatgaaa
     7861 aaattttgca atattttctg gttctataaa tgatttgatg atattttcag ggacattata
     7921 taaattttta aaagttccaa ttaaatcttg atcttctgcg attactcttt ttgacatagc
     7981 taagatagga gttcctgtat agtgaaatcc cattggaaat aatacattat agcctttcat
     8041 tctcatatat ctagcatgaa catcagctag ggaatatgtt cgcccgtgtc ctatatgctg
     8101 tggagaatta ggatagggaa atgcaacagt tatgaaatac ttttttttat tagtattatc
     8161 ataatcggct tcaaaaagat gttcttcttt ccatctagac ttccattttg cctctagttc
     8221 tttccaatta atactattat catttatttc tctactattc agattatttc acctcttgag
     8281 tgtttcatta acatatattt ctgatgaatg taagacctct tcaattctat tcttaaatct
     8341 acctccgcct tgaccaaact ttgaatctcc tcctccagag ccgcctaaac taatagagaa
     8401 gacttttgct atttttcctg cattaaaacc atattttctt gcagtttcac caacaaaaac
     8461 tattacgcgt atgccttctc cttctccttt ggagattagt gaaatatata tgagaaaagg
     8521 atcaaggtta atggattttt ctcctattgt aatatggaat tcttcatcta attcatcatc
     8581 atatgttgta aataatttca ccccgtttga tgatatttgt ttactattag tggatataga
     8641 tttggctaaa attggagaag tttttcgtaa aagaattttt atctttgttt taagcttttc
     8701 tgattcatct aaatattttt tgaatgaatg tgttagtttt tctttactag aacctaatga
     8761 ttgagtaata aatgaaatat ttctatcctg atttttaatg taatctatag cagcctgtcc
     8821 agcaacaaat tctaatcgaa caacaccatc ctgaattctt tctgatttga gaattttaat
     8881 taaacctaat tcacctgttc tattgacatg tgttcctcca catgcttcaa tgtcaaattc
     8941 acttattttc actattcgta ctttatttga tggaactact ccaccttgat agatacgaaa
     9001 ggaaaatttt tgttcacttt ctcctctatc atattctttt atatttacat caaggttttt
     9061 cattacaatt tcgttagcag ttttttcgat tctttcaact tcttctttat ttaatgcaga
     9121 atggtgagtt atgtcaagtc ttccataatt aacatcttta aaagcagagt tttgccacac
     9181 ccatgaacca agtgtagttc ttgatgaaga atttaatata tgagtagctg tatgatgttt
     9241 tgttattgaa tcgcgtctta catcatctaa cctacattta attcgtagtc cctctgaaag
     9301 agatgataaa ttttctaccc gatgaaggac aatatctcct tgttttatga cttctaagac
     9361 tttaacatcg ccaatatttc ccttatcagg ttcttgaccc cctcctctag gatagaaaga
     9421 tgtttgatct aaaattacat aattttcttt tatttttatg atttgggcat aaaattctcg
     9481 gatggaagaa tcttcgtaat ataataatct agtaggcgat aggccttcca atccttcgat
     9541 tgacggatgg taaagagatt tttggttgat attatgtaat tctgatagtt tcacgtaaaa
     9601 atttgaaggt atatcggtta ttacattgat ttctttaaga aagtcaggag taataccatc
     9661 ggattcatac aatcttataa gatcatctac agttagttgt ttgttattct tacgtattgt
     9721 agatgcgata tcattcatac gttgtcttgt gcttacatat ctatcagatt caagtttaag
     9781 aatggtataa acctcttgct tattttcctc tagttcagga tacatatttt tcagatattc
     9841 tacgtgtgta tcaattagat cttcaatttt tatattccat ttgaatcgtt caataataga
     9901 taaaactcta cgtaaaagaa ttctaagatt ataacctcct cctacattag aaggtaatga
     9961 tccatcagaa atagcaaata acaatgtcct cgtgtgatct gctattgtat aaattgcctc
    10021 atatggagat acaatttttt cgagatgttc aaatggtatt ctcatctctt gagcaatagt
    10081 ttttttcaat tgttttattt tatcagttga atcatcttca ataatgttga atttactaga
    10141 tactaattta aaatagtttg atagaaaatt ttcatccttt tgaacttcaa tagagttttc
    10201 taaatatttt atgactggtc caaaagaaca atcatagctg gttggagtac ccatagtaat
    10261 ccatgataat cgttctaagc ctgcacccat atcaataatc ttatttttca taattctgta
    10321 attattttca tctccttcga attctgtaaa aacagcatta ccaagttcaa gaccccttac
    10381 aaaatactct aaagaacacc caaaagctcc tgcacccatc caaatatctt ccacaaaagt
    10441 aatctcttca ggttttattc ccaaaacatt agttaataat ccaaagtcta ggtcaatgca
    10501 atgatccttc caatatcctc caggtgcatc tgcattacat gtttgaccaa tcatacagaa
    10561 actagtgtaa tgacggccgc ttacccccac attttcaacg tcattgaatc ttaaacacat
    10621 ttgtggtact actaaaggat ttgatggcaa ttcaaaaata actttattat tcattattct
    10681 ttgaaaatcc actatagatg caattgtaaa ataaagatct tctcgccacc tacacactac
    10741 agggtatctt cttacaatag agtgatcatt tgattgaaaa tatttttcaa tttccttcca
    10801 tgctcctata taatctagtc ttttatttgt aggaggattt cctataaagt tataggcctc
    10861 atgatctgga cagatatttt tatctataag cgaccaaaaa aatttttcac aagatataca
    10921 tttgtttcgt ttaaaaccta atctatcaaa taaggaaact ctataatatt tatcaggatt
    10981 agatgaaaat agagtagaaa tctctttttt acccaaatta tgctaacgat tattattatc
    11041 tatattaatg cttttatgaa tacagaaata aaaactaaaa ctcattaaat taaatgaaaa
    11101 gaaattaatg ttaattcttg gttaaagatt cttcgatgaa tttttgatag gcttctttag
    11161 gagcagcgcc gacagttctc gcaatttctt tgccgtcttt gaataatagc aatgatggta
    11221 tagagtgaat tccatatttt acggcaatct cttgattttc atctacatta agctttacag
    11281 tttttacttt tccttcaagt gtttttgata attgttctac ggctggtcct accattctac
    11341 aaggtccaca ccattctgcc caaaaatcaa caaaaacagg taattgtgat tgtaatactt
    11401 ctttttccca tgatgcattg ttcacttgag ttacactatg ttcagttccc atatatttta
    11461 atttgagtgt aggttatttg aatcatactg aatacagat
//
LOCUS       DASVEV010000245        11016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1675009, whole genome shotgun sequence.
ACCESSION   DASVEV010000245 DASVEV010000000
VERSION     DASVEV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675009"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1060)
                     /locus_tag="VF222_10610"
     CDS             complement(308..1060)
                     /locus_tag="VF222_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142916.1"
                     /translation="MYFHKYIFAVIFLGFVFIITLESPFSIQLADFLTNNNIVEEALA
                     QPPSTNTSIIMNNNSEEQNNSKLIKAFGHFANNQIKDGIVTWIQGGFWELEISDTTNS
                     NVNNNNIQLINNNSSKANFIANFTMIKPDGSLSHNHNIQNYISDKVILTDKDIVVMGI
                     ADIYSNDKHNEYKSVPLTIHLMGNKVLGLTIDVGKTKGHFSSSNEMFGTLISGIGLDT
                     SADNSINKTMMHHDTAATNDTDEKESMTMMQH"
     gene            complement(1558..1986)
                     /locus_tag="VF222_10615"
     CDS             complement(1558..1986)
                     /locus_tag="VF222_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7142917.1"
                     /translation="MKDKPFNFHGAKFTIKVLTSETNGSYTILDVIHPPNIGPAFHLH
                     PKGSETFYIVEGEYEFILDGKSVTGKPGDVIFVSKGVPHRFVVGDKGGHALVISPPEL
                     EFYFFKVSELLDKGEVSYETESSIGKQYGQVFLDNTKHWK"
     gene            complement(2350..3849)
                     /locus_tag="VF222_10620"
     CDS             complement(2350..3849)
                     /locus_tag="VF222_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX7142918.1"
                     /translation="MTSKSQIIKTRIVIIFLAIFSLVSSYSIFYSQTVNGATTNSNIT
                     ITSSQELSTIMSQISNSDKPEDIATLAYLWGYPLITSQRSFDYFTNPNTPPVVGQGPA
                     NEMNCAHQLVNASFTDIVTPNDDTLYCQSWMDLTKEPVVLKVPSVQDRYISFQFLDAY
                     TNDYTYLGTRASDGSEGTYLIAGPNWDGQVPQDMTKIWSPTNIAWIINRILVTGDSDL
                     PNVHDIQDKISLIPLSVFEGNTTNTQAADETTLSSQVPIKPQPANIPTTGIKLYDELG
                     QAMINNPLNPPDPSLVAKLASIGIGPGKIPSVESNDTIKTALQTGIQEGEKLIDEKLA
                     NIGTVVNGWSVNTQTGIYGNDYLTRAAITKLGFGANIPQEALYPTTLTDSQGKPYNGA
                     NKYAIHFDPGQTPPVDGFWSITMYNEEKYFYDNPLNRYSIGKYTDGLKTNEDGSLDIF
                     IQNQSLGADKESNWLPSPADGFYMVLRMYLPTEQVLNGTWTPPPVKVIE"
     gene            complement(4162..4341)
                     /locus_tag="VF222_10625"
     CDS             complement(4162..4341)
                     /locus_tag="VF222_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142919.1"
                     /translation="MTLVDLNPVEINLIIDLLKFNIDDLSLGSPNRRIPEQTLKEWKN
                     HIQTVIYKLEKAVEK"
     gene            complement(4533..4991)
                     /locus_tag="VF222_10630"
     CDS             complement(4533..4991)
                     /locus_tag="VF222_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142920.1"
                     /translation="MFVFKECHKHLKEYSIRFNFQVFESNTDPVKTPILVVDKKYIPE
                     ADAKSINEINYSVQCSMLRGHTFFNLDLQNEITGQETHFSICFCPLEYAGNNELYGRM
                     VGFINIYKQLGFLMVTSPESLDKLNGKTGEQIADMVVNGEINNLAVSWKI"
     gene            5025..5213
                     /locus_tag="VF222_10635"
     CDS             5025..5213
                     /locus_tag="VF222_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142921.1"
                     /translation="MAKLLCGTDKCNKVIDVSISDQNLKHKCTNCNTIFCSEKHHKEH
                     TCEFTIKEMQEIGQYYKI"
     gene            5641..6723
                     /locus_tag="VF222_10640"
     CDS             5641..6723
                     /locus_tag="VF222_10640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7142922.1"
                     /translation="MQSEKSTNKINEQKLHDFMLKSVGDIASTMSAMLMIIGDRLGLY
                     KAMAESGLPLTSEELAKKTNTNERYIREWLANQAAGGYITYNKSDGKYFLPPEQAMVL
                     ADENSSVYMHGAYQSIRSFFKDEDKFVKMFQSENGLRWGEHHHDLFEGTARFFKPNYI
                     GNLVSSWIPSLEGVEEKLKQGAKVADIGCGYGISTTLMAKAYPNSKFYGFDNHSPSIE
                     QAKEQARKEGVIENIEFSSVSANDESIGNDYDLITFFDCLHDMGDPIGAMKFAKQSLK
                     PDGTCMIIEPMANDKVEENLNLVGRIYYAASTLVCVPNSLADNGPALGAQAGEKKIKE
                     ISEASGFTKFRRATQTPFNIIYEAKP"
     gene            6932..7474
                     /locus_tag="VF222_10645"
     CDS             6932..7474
                     /locus_tag="VF222_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142923.1"
                     /translation="MSDGSVMTMNGISMSNLKNNSSYVKEVKILNVNTKIEINPFHSG
                     FNTFKITFTDTNGQPYSKILTVRMIFKNDQADIGPITTKLTKVSPGVYTIREGYISQP
                     GEWNVAMAAQRPSDYDLNYRFNPTVNSSSSSSGLTQTNSSNNADIAYTMNNTDSRFVP
                     VFDYFTLLAITLAVIIGITS"
     gene            7780..8874
                     /locus_tag="VF222_10650"
     CDS             7780..8874
                     /locus_tag="VF222_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7142924.1"
                     /translation="MKNDYAYTRRFILLVGIIGFFMSLTTSYATTTTTNTTNTSNTSN
                     TTNTSNTTNTSNQEEVVLEDKLNVVTSVAPITNIVQNIGGDKINLVGLVPEGVNSHTF
                     ELKPSDTIKIHDADLVIIDGLNLETNVEKIAENVISKNPNNIQLLKLGDKTISKEDWV
                     FDFSFPKEKGDPNPHLWLNVEYAMNFANLTRDKLIEMDPSNSQYYIDNSNKYTTLLKQ
                     LDTGIKKSVETIPLENRKLLTYHDSWAYFAPHYGMTVIGAIQASDFSDPSPKDIADLI
                     DQVRIEKVPAIFASEVFPTNIVDQIAKEGNVAIVETLSDDDLPGNPGDNNHSYVGMMI
                     ENMRNMILPLGGNIDSLKDINPQNILHITS"
     gene            complement(9084..10196)
                     /locus_tag="VF222_10655"
     CDS             complement(9084..10196)
                     /locus_tag="VF222_10655"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7142925.1"
                     /translation="MIQKILNNNVILLMSLIIVYVIGNNIFYLSAYADLPDEINKIQN
                     CKNSKIIDKAIPNSNSNDQKNDNNDDTKPIENFHHIKKFNSNGELIKGWGVKGTGNGE
                     FLHPHGIGSDSKGNIYVSDAVLCNIQKFDSNGHYLLKFGSRGNGPAEFLQPESIAIDK
                     NDNVFVADYATQHVQKFDSNGNFITMWGSKGTKTGQFMKPWGIGVDSVGNVYVGDQEN
                     PVIQKFDNNGNFITKWGSYGSKAGQFVHIHDITLDSNDYVYATDGRDNSRVEKFDSDG
                     NFITMWGSKGSKEGHFIEDHGIAVDKSGYVYVVDTRNIRIQKFDSDGNFITMWGSLGC
                     KDDQFLIPHDITIDPKGNIYVSDSGHVHFRDNYLCK"
BASE COUNT         3843 a         1669 c         1641 g         3863 t
ORIGIN      
        1 cagttcccgc atctataaat agatgatata tcaaaacctt ttgtaagtat attatagata
       61 gtatcaacaa atttaacttc tacagggtca gtagcattaa aaatttttgg tttactcttc
      121 ataatatttt ttgtatttca gtgttaaata aggttttgaa agtaaattaa tttcattaaa
      181 aaccatagcc ctccagccaa acagaagaag tagagagagc gacagataaa atcaattcgc
      241 tatattcttt aatttttatt tatacttgtc ttgacttcca ttctttaagt ggtaaaatac
      301 agaaatttca atgttgcatc atagtcatag attctttctc atcagtatca tttgttgcag
      361 cagtatcgtg atgcatcatt gttttattaa tagaattatc agcagatgta tccaatccta
      421 ttccactgat taatgttcca aacatttcat ttgagctaga aaaatgtcct ttagttttac
      481 caacatctat agtaagtcct aaaactttgt ttcccataag atgaatagtt aaaggaacac
      541 ttttgtattc attatgcttg tcattagaat agatatctgc tattcccatt acaactatat
      601 ctttatcagt aagtattacc ttatctgaaa tataattctg aatattatgg ttatgcgaaa
      661 gactaccatc tggttttatc attgtaaaat tagcaataaa attggcttta ctactattgt
      721 tgttgattaa ttggatattg ttattgttaa cattactatt agtagtatca cttatttcta
      781 attcccaaaa tccgccctga atccatgtaa ctattccatc ttttatttga ttattggcaa
      841 aatgtccaaa ggcttttatc agtttagagt tattttgttc ttctgaattg ttattcatta
      901 ttattgatgt gtttgtagat ggtggttgtg ctaacgcctc ttctacaatg ttgttatttg
      961 ttaaaaaatc tgctaattgt attgagaatg gtgattctag tgttattata aatacaaatc
     1021 caaggaatat taccgcaaaa atatatttat gaaagtacaa agtaaaattc ttcattttgt
     1081 tatttttata ctaatatcta taaattccta ttctcaaaaa tattttatat tcctttttag
     1141 atattattca aataacaact tctaaatttg caaatgtctc catattcatt cacaagacta
     1201 aaataaaaaa ttaggagtta tcaaagtaca atactcaagt taacatactg aatttcaatt
     1261 tactatacaa aatcaagaat gttagatatt ttagatttct ataattttgt gaacccagac
     1321 taatcgctta ttgaaaaaaa atattgggat attaatctct atatccaaaa ggccataata
     1381 gcatttttaa aaatattcta cctacgaaaa tatctactaa gagtgacaag aaattcatta
     1441 ttagagttaa agctaaaaat tatataagcg tttaacttct gtttgataag gaattttttt
     1501 cttttattcg ctaaccacta agaaatctca attatatgct cctcctaaac attttgacta
     1561 tttccagtgt tttgtgttat ctaagaaaac ctgaccatat tgttttccta tacttgattc
     1621 tgtttcatat gaaacttcac ctttgtcaag taattcactt actttaaaaa aataaaactc
     1681 aagttctgga ggactgatta caagagcatg tcctcctttg tctcccacaa caaatctatg
     1741 tggaactcct ttggaaacaa aaattacatc tccaggttta cccgttactg atttcccatc
     1801 taaaatgaat tcatattcgc cttctactat gtaaaaggtt tctgaacctt taggatgtaa
     1861 atgaaatgca ggacctatat taggtggatg aattacatct aggatagtat agctaccatt
     1921 cgtttcagaa gtaagaacct tgatagtaaa ttttgctcca tgaaaattaa atggtttatc
     1981 tttcacaaaa aatattttac gttttaaaaa tatatctgtt aatttttcaa aaacagagaa
     2041 ttgttaagga gcggaatcat ctacgtaaga aaggctctac tatttggtat ttatatttta
     2101 ttattagaaa cttaatttta aatagatttt gaaatatcaa gagatgtaaa ttggtagaaa
     2161 tatgccaagg agggagtaat accgatagcg gtgtaaaatt agagtctaat atgtagtaat
     2221 aaaagagaat ataagattta ttattttatg tacttttgaa atatttaata ttaagaataa
     2281 taattagaat caaaaaaagt tatgtgggat atataatgtg gaatcaccga ttgcattttt
     2341 gatgaatctt tattctatga cctttacagg tggaggtgtc caagttccat taagaacctg
     2401 ttctgtcggc aaatacatac gtaaaaccat atagaagcca tcagcaggtg aaggtagcca
     2461 gttggattct ttatctgcac caagactttg attctgtata aagatatcaa gtgagccatc
     2521 ttcgttagtt tttaatccat cagtatactt tcctatcgaa taacgattta gtggattgtc
     2581 atagaaatat ttctcctcgt tatacatcgt gattgaccag aagccatcta ctggtggagt
     2641 ctgtcctggg tcaaagtgaa tggcatattt gttagcaccg ttatatggtt ttccttggct
     2701 gtctgttaat gtggttggat agagagcttc ttgtggtata tttgcaccaa atcccaattt
     2761 agtaatagct gctctagtaa gatagtcatt tccatagatt cctgtctgag tattaacaga
     2821 ccacccattt actacagttc caatatttgc taatttttcg tctatcagtt tctcaccttc
     2881 ctgtattcca gtttgcagtg cagtttttat tgtgtcattt gactcaactg atggtatttt
     2941 tccaggtccg atacctatac ttgcaagctt ggctacaagg ctagggtctg gaggattgag
     3001 tggattgtta atcattgctt gtcctaattc atcatacaat ttgataccag ttgttggtat
     3061 gttagcaggt tggggtttaa tgggaacttg gctagagagg gtcgtttcat ctgctgcttg
     3121 tgtatttgtt gtgtttcctt caaatactga aagtggaatc aagcttatct tatcttggat
     3181 atcatggaca tttggcaagt ctgaatcacc tgtaactaga atcctgttaa taatccatgc
     3241 aatatttgtt ggtgaccata tcttggtcat atcttgtgga acttgtccat cccagtttgg
     3301 accggcgatt aggtaagtac cttctgagcc atctgaagca cgcgttccaa ggtatgtata
     3361 atcattagta tatgcatcca ggaactggaa tgaaatgtat ctgtcttgaa cggaaggaac
     3421 tttaagaaca actggttctt ttgtcaaatc catccatgat tgacagtata atgtatcatc
     3481 gttaggggtt actatatccg tgaaacttgc atttacaagt tggtgtgcgc agttcatttc
     3541 atttgcagga ccctgtccaa cgacaggagg agtattagga tttgtgaaat agtcaaagct
     3601 tcgttgtgag gttatgagtg gataacccca cagataggca agtgtagcaa tatcttcagg
     3661 tttatcagaa ttagaaattt gtgacatgat tgtactcaat tcttgagaag atgttatggt
     3721 aatattcgag ttagtagtag caccatttac agtttgagaa taaaaaatac tgtatgaact
     3781 aaccaaagaa aatatcgcaa gaaaaataat aactattctt gtcttaatta tttgactttt
     3841 tgaagtcatt aaacaagtta ctataggagg aatatataaa cactcataac aatcactata
     3901 tagtcatgtg atgattagtt tactatcact ttatacaaac ttatataatt ctaatattaa
     3961 aattaagaat attttatttt atgttatatt gatgtcaaag attaaaaata tcataataat
     4021 aaagcaacaa aaacaatgat acttgccatt tatgttatta aaatagtatg ttatttataa
     4081 tatataaatt tctaatacta tctcccatct cctattacag tatctttaca tgatataaaa
     4141 atggaaaatg tttacagccg ctcatttctc tactgctttt tctaatttat aaataacagt
     4201 ttgaatatga tttttccatt ctttcaatgt ttgttctgga atacgtcgat tgggagatcc
     4261 taggcttaag tcgtcaatat taaattttag gagatcgata atcaaattaa tttcaacagg
     4321 atttaaatct actaaagtca tctctctctt taaatctcct tgaatattgc ttcacaagac
     4381 ttgcagaaaa ctctagtgtc cagattcaag aacttgttgt ccatttctat caatacttta
     4441 tgaacgatgt caaagttatt cagatcttta atactgaatg atattttaag atattgatcc
     4501 atatattgtt agtagtttca ataggtgaga tatcaaattt tccaagaaac tgccaaatta
     4561 tttatttctc cattaactac catatctgcg atttgttctc ctgtttttcc attcaactta
     4621 tctaacgact caggagatgt taccatgaga aaacctagtt gtttatagat attgataaat
     4681 cctaccattc gaccatagag ttcattattt cctgcatatt caaggggaca gaaacaaata
     4741 ctgaaatgag tttcttgtcc cgttatttca ttctgtaaat cgagattaaa gaaagtatga
     4801 cctcttaaca tcgaacactg aacagaataa ttaatttcat tgatagactt tgcatctgct
     4861 tctggaatat actttttgtc aactactaat attggtgttt taactggatc agtatttgac
     4921 tcaaatactt ggaagttgaa ccgaattgaa tattctttca ggtgcttatg acattcttta
     4981 aatacaaaca aaataataat tagtagtaaa ataaaggata gaaaatggct aaactattat
     5041 gtggaacaga taaatgtaac aaagtaattg atgtttcaat atctgaccag aatctaaaac
     5101 ataaatgcac taattgtaac actatttttt gttctgaaaa acaccataaa gaacatactt
     5161 gtgaatttac tatcaaagaa atgcaagaga taggacaata ttataaaatt tgaactctaa
     5221 caataaccct cctatttgta ttatttgtaa aattaatctt gtccagcaag attctactac
     5281 taatttattc aagattcttc taaatttact gaagtgattg atgttgctcc caccatgatg
     5341 tatttgtatt gaggttaata ttgtttacag gactggctta gaggtgtgtg aattgttttt
     5401 gacatcatat ttttggccca tttttaaatt aacaattttt tgttgcttcc ttcaggagtt
     5461 gaatagacac agtttcaatg ttcaatcaat gaatctagat tgaatagagt ttacagaaga
     5521 ttacaatatt gctaataata ctaatttaaa ataagaatta atcgtaatta aaataaaaaa
     5581 ataaaaaatt agtataaaaa agatacgtat aatacgttaa aataatattt aacaataaca
     5641 atgcaatcag aaaaatctac caacaaaatt aatgaacaaa aactacacga ctttatgcta
     5701 aagtctgtcg gagacattgc aagtactatg agtgccatgc taatgattat tggagataga
     5761 cttggtttat acaaagcaat ggcagaatct ggactgccgc ttacatcaga agaattagca
     5821 aaaaagacta acactaatga gcgctatatt agagaatggc tagcaaatca agccgctgga
     5881 ggatatataa catataataa atcagatgga aaatactttc ttccaccaga acaagcaatg
     5941 gtattagcag atgagaatag ttctgtatat atgcatggag cctatcaatc aatcagatct
     6001 ttttttaaag atgaagataa atttgtaaaa atgttccaaa gcgaaaatgg attgagatgg
     6061 ggtgaacatc atcatgatct ctttgaaggt actgcccgat tttttaaacc taattatatt
     6121 ggaaatcttg tgagttcatg gattccttca cttgaaggag ttgaagaaaa actaaaacaa
     6181 ggtgctaaag ttgccgatat tggatgtgga tatggtatct caacaactct tatggccaag
     6241 gcctatccaa attctaaatt ttatggtttt gataatcata gtccatctat tgaacaagct
     6301 aaagaacaag caagaaaaga aggagtaata gagaatatag aatttagttc agtatcagca
     6361 aatgatgaat caattggtaa tgattatgat ctaattactt ttttcgactg tcttcatgat
     6421 atgggtgatc ctataggagc aatgaaattt gcaaagcaat cgcttaaacc agatggtacc
     6481 tgtatgatca tagaaccaat ggccaatgac aaggtagaag aaaatcttaa tttggtaggc
     6541 agaatatatt atgcagcatc tacccttgta tgtgtcccaa attctttagc agacaatggt
     6601 cctgcattag gtgcacaagc aggtgagaaa aaaataaaag agatatcaga agcatctggg
     6661 tttaccaaat tcagacgtgc aactcagaca ccatttaata taatctatga ggcaaaacct
     6721 tagccataaa cattatacaa caataacata ttttctttat tttttagatt tattgcagat
     6781 tatgaatttt cattgtgatt aaacagaaga gtagtataga acaattattt catctttatc
     6841 tcataatatt gaaaaagtaa aataataccg cttttttctt agtccataaa tgtagaaata
     6901 aatgtaataa ttctaatgga atagtatgac aatgtctgat ggtagtgtta tgacaatgaa
     6961 tggtatatca atgagtaatt taaagaataa tagcagctat gtcaaagaag taaaaatatt
     7021 aaatgtaaat acaaaaattg aaattaatcc atttcattct ggatttaata catttaagat
     7081 tacttttacg gatacaaatg gacaaccgta ttctaaaata ttaactgtaa ggatgatttt
     7141 caaaaatgac caagcagata ttggaccgat aaccactaaa ctcactaaag tctccccagg
     7201 agtttatacg attcgtgaag gatacataag ccaacctggt gaatggaacg tggctatggc
     7261 agctcagaga ccatcagact atgatcttaa ttatagattc aatcctacag tcaatagttc
     7321 ttcttcatct tcaggcctga cgcaaactaa tagtagtaac aatgctgata tagcctatac
     7381 tatgaataac actgattcca gatttgtacc tgtatttgac tattttacat tgcttgcaat
     7441 tactttagct gtaatcattg gaattactag ttagtatttt taaaaaaaat aaagagaaac
     7501 ttagaaaaac cattagaact cttaaatcaa acatataatt aactctttta gttagtttga
     7561 ttgatatagt tccatcttat tgatatttaa tatattgtat agaggaaaat tatttgtctt
     7621 aattattgat atcatcaaaa aacagatgct aagacaaata atattactaa tcaatctcta
     7681 gaacgagata taaagagatt tactttatct gtctaaacac aactcataag atatattatt
     7741 ttcagaaaat attaatataa taaaaaaatg attgtttgtg tgaaaaatga ttatgcttat
     7801 actagaagat ttattttact agtcggaata attggatttt ttatgtcact tacaacatcg
     7861 tatgctacta ctactactac taatactact aatactagta atactagtaa tactactaat
     7921 actagtaata ctactaatac tagtaatcaa gaagaagttg ttctagaaga taaacttaat
     7981 gtagtaactt cagttgctcc cataactaat atagttcaaa acattggagg agacaaaatt
     8041 aatttagttg gattagttcc ggagggagta aattctcata cctttgaact aaagccttca
     8101 gatacaataa aaattcatga cgcagatctc gttatcattg atggtcttaa cttagaaaca
     8161 aatgtagaaa agattgcaga aaatgtaatt tctaaaaatc caaataatat tcaattatta
     8221 aaactaggag ataaaacaat atccaaggaa gattgggtat ttgactttag tttcccaaaa
     8281 gaaaaaggcg atcccaatcc tcatctttgg ctaaatgtag aatatgccat gaattttgca
     8341 aatttaacac gtgacaaatt aatagaaatg gatccttcta actcacaata ttatattgat
     8401 aactccaaca aatatacaac gttactaaaa caactagata ctggaattaa aaaatctgtt
     8461 gagacaattc cattagaaaa tagaaaattg cttacttatc acgattcgtg ggcttatttt
     8521 gctcctcatt atggaatgac agtaatagga gccattcaag cctcagattt tagtgatccc
     8581 agtcctaaag atattgcaga tctaatagat caggttcgta ttgagaaagt gccagcaata
     8641 tttgcatcag aagtatttcc taccaacata gttgatcaaa tagccaaaga aggaaatgtt
     8701 gctattgttg aaactttgag tgatgatgat ttgcctggta atcctggtga taacaatcac
     8761 tcgtatgttg gaatgatgat agagaatatg agaaatatga tactaccttt gggtggcaat
     8821 attgactcgt tgaaagatat taatcctcaa aatatcttac atatcacatc atagatatat
     8881 cttttttata aaataagaat gatatcaaat atacttgtaa aactctcaga tctttaggta
     8941 ttatccaaaa ttaaagttat ttttagttca attaaattaa tatttaattc aattaaatta
     9001 agtgatgtaa tccattgata atctatttta ttgcaaactt aatttttatg ctatcttaca
     9061 atacacatat ttaatctgtc tggtcattta cataaataat tgtctctaaa atgtacatgc
     9121 ccactatcac ttacataaat attacctttt ggatcaattg ttatatcgtg aggtattaga
     9181 aattgatcat ctttacatcc aagtgacccc cacattgtta taaagttacc atcactgtca
     9241 aatttctgaa ttctaatatt tctagtatcg actacatata catatcctga cttatctact
     9301 gcaattccgt gatcttcaat aaaatgacct tccttacttc ctttagatcc ccacattgtt
     9361 ataaagttac catcactgtc aaatttttca acccgagaat tgtctctgcc atcagttgca
     9421 taaacataat cattagagtc gagtgtaatg tcatgaatat gaacgaattg tcctgctttt
     9481 gatccgtacg acccccattt tgttataaaa tttccgttgt tgtcaaattt ttggatgact
     9541 ggattctcct gatcacctac ataaacattc ccaacagaat ctacacctat tccccatggt
     9601 ttcataaatt gaccagtttt tgtaccttta gatccccaca ttgttataaa gttaccatta
     9661 ctgtcaaatt tttgaacatg ttgagtagca taatcggcaa caaatacatt atcgtttttg
     9721 tctattgcaa tgctttctgg ttgtaaaaat tctgcaggac cattgcctct actgccaaat
     9781 tttaacaaat aatgaccatt actatcaaac ttctgaatat tacaaagtac agcatcactt
     9841 acatagatat ttcccttgga atcggatcca attccatgag gatgtaaaaa ctctccatta
     9901 cctgttcctt tgacacccca acccttgatt agttcgccat tagaattaaa ttttttaata
     9961 tgatggaagt tttctattgg tttagtatca tcattattat cattcttctg atcgttgcta
    10021 ttagaatttg gaattgcctt atcaattatt tttgaatttt tacaattttg gattttattt
    10081 atttcatctg gcaaatctgc atatgctgat aaatagaata tattatttcc tattacgtat
    10141 acaataataa gagacatgag caggattaca ttattattta aaatcttttg tatcatatct
    10201 gttcaataat aagtacttag aagtacaaat aataacaaat tgaagttttt gttgaaagat
    10261 agaaatccaa tcgattttct tcctcctttt ctatttctat ttttttccaa tacaatattt
    10321 ttattttcta attcattata taaataatag taaacgaagt taacaaattg tattgatggt
    10381 ataaaatatt cacactaaaa aaacatacaa attatggctt ccttgttagg ggatatgcct
    10441 atggtgtttt ggtacttata tcgtggtatc caaggtattt aaaaatcagt aaacaaggtt
    10501 aacaatcaat tatataagtg gaattgtcat ggcatgaacc ggtagtagtt ccttcacgaa
    10561 gatgattcag acgatgacgg tttgcctatc taactgagga tattcaaagt taatatggta
    10621 ttattgattt taaactcttt agcatcaact tgatgaattc caattatata ttaattaata
    10681 caaatgagac tcctgtaatt tcaaattata tttgtatggc aactacaggt ttgtagatat
    10741 tagtagcatt aatagtaaaa gtaggtagca taaaagatta ttgattttag tcaggaagga
    10801 aaaagagaaa aagaacaaaa actaaatatg gttgaattat tgtaatagct tacttcttct
    10861 aatcaacaat aatcaaaact tttgatattg aataaaatta taaaattatt tatttcaata
    10921 cttcttaaac ttttttcaat atttttgaaa atgttttaat tagataacta taatataatt
    10981 caacaattaa ttttgaaaat accaaattta ttctta
//
LOCUS       DASVEV010000246        16221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1091421, whole genome shotgun sequence.
ACCESSION   DASVEV010000246 DASVEV010000000
VERSION     DASVEV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16221
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091421"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF222_10660"
     CDS             <1..1044
                     /locus_tag="VF222_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7142926.1"
                     /translation="TTTSTSNDDDSISSPALTTNQDDLNKTIEGMLVKIQEIAEKIQN
                     NTSKMIVDNEFLSQEEVADMVLTLSDDTISKLINGRIIPNDRVFYPVKPIVGTSIDDN
                     NLQNLKEKVVVITTTSSKEKDVQRITNLANKLNETGVKQVIILSKNSSDDTKFYKDFH
                     SHSLDLADEEKVKRIFNTAKTKFGSIDSVIHITGEYDYSRSLSSLSREEWDVLVENFI
                     HIPALITREAVKFMSPADAIQKPSQFKDSKGIIVILGPDSPYGKKISGLSRARSEVFR
                     GALRPYTATVNQELSDVLGSKIKLNLILPGNIEGIESNIENVTTSILNLIAGSSLNKN
                     ESIFYIDEGKKEK"
     gene            complement(1260..2087)
                     /locus_tag="VF222_10665"
     CDS             complement(1260..2087)
                     /locus_tag="VF222_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142927.1"
                     /translation="MGENDNSEILKNNSSLMVTTTTITTTAGSRAAAATVMSAEATAA
                     AAAAAAAATATATDEYKQVKTNNFNSYSNNSIQNCHSSQFSPDDSEFHLIKYNIPLRP
                     KIKQIQFRISDEILKLQNIFNDLKTQDEELIKKLDISIRENNIQYSSLISSELNGIRK
                     SSRAIWTSKIVLDTLHNQICNISSFKDLVEKLTPSLTVMKSIRSLLISHIRESDHEFK
                     NISDLLCDVLFNAGQLGGSLINFKIANNAAVDITDDAMFKAENKIKIEFLPIPKMIT"
     gene            2270..3874
                     /locus_tag="VF222_10670"
     CDS             2270..3874
                     /locus_tag="VF222_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7142928.1"
                     /translation="MNQRHIHDDDYSIQDPIINNLENNKKVSGPEKQYFSHDDSQYSN
                     NNNGSDGDTTKCVSNISIENNNDLIGNLEEFGLSQYEARAYLTMIGKGSLSASEIAYY
                     SHLPRTKIYLTLKKLEKKGLSVISQQKPLICSAINPTEAFEEIVMIQEKRLMNMKKAI
                     DKLQKINDKSNYLKGLEEKRYFILDPQSTFQKLSTLIENSRTSIIAILDVWGLRLISQ
                     CKSLLIKAITKGIKIEFLISNQCIGSEDLSFLPNEILIKVGNVFSNIIILDSGNIINI
                     DGGNGKAALFTSIDILGFSYMKIFEEEWKKAIEIKNLNKTESLNQLTAVQLIRFIEDL
                     SRFSTSQYLYSIYLSILSSLSIDNRSDGSVSSSQSSPPPPPFSLTHHYPTPPSYQSQQ
                     KEGEEKEDSATTILSNKKDINERIFDYNSKINFGKNHHHVSFLNSLERYGVNLTDFTI
                     EELIQIVDLILKMNYSGELKFDGNNNILSIHSKGKNKSLIILTILLSLYFINLNYEID
                     ITPKISEISDEITINTFHIKYSKSLH"
     gene            complement(3980..4870)
                     /locus_tag="VF222_10675"
     CDS             complement(3980..4870)
                     /locus_tag="VF222_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7142929.1"
                     /translation="MESPELVTINDDMIKRVLVPRKNSSRKGDNGIVLVVGGSRIYHG
                     APVLASIAALRSGTDLVYTAVPNVNVESTRSYSPNLIVIPLVDQRLTMGSAARLIKAL
                     PKKVDVATIGMGMSIAKKEALLSLVEKLLKNGTKLVLDASALIPEILPKIKGSNCIIT
                     PHGGEYMRIFETELKNSNNNNSENEQILDVFNNAKKYGITIILKGWKNIISNGELTTV
                     IERSTPAMTVGGTGDVLAGLVAGFYSKLDSFDASCTAVYFNGIAGIMAYQKSGLHIVA
                     SDLLDNLPEALKKFDQIENN"
     gene            5046..6038
                     /locus_tag="VF222_10680"
     CDS             5046..6038
                     /locus_tag="VF222_10680"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7142930.1"
                     /translation="MSKPIEIVFSEKKMPSLNSPHLICGFPGTGLVGKLAVDYLIKEL
                     GAIHMADLFSSYFSPQVVIQVDGTTNIVKNSLYYVKKNNNNNNNHNNKTSKNKKEIKD
                     ISKPEVQSDQSINDPAASPSPLPQSTVSLNPTNTTVSPLSSQDLILLTGDSQPVVPGS
                     EYVLSEQILDLITKFKISNIYSLASYVTGTFVNDPKIYGTATNPEMIKSFRSFNISTL
                     DNGNITGMNGLILGLGKLRGIEGICLLGETSGYVIDAKASKNLLEILNNVLGININMD
                     EMNKRSKDTEILIKNLEQQMMAKSGQFPQGVPTEEQEEELMFPQNRKSNMGYIS"
     gene            6175..6867
                     /locus_tag="VF222_10685"
     CDS             6175..6867
                     /locus_tag="VF222_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142931.1"
                     /translation="MFNYFTSLLLLSSVIFFILISFDLEFTNSSKISFGQNSNLIDST
                     NISSSINNSKNETPTTTTTTAGEAATDNWLTYSNNKFELKYPPDWDVEKKLSKFHVLD
                     FKLIKHNPFSFIGISFSSLPDSEKFTNEIILDEAEKFGGMSPPSSSSGYEFYQVLEKE
                     PNKYTIDANPSSFHIVKYKYKDNDLEGKLMEIFSLIDKRLFTLTYQSTIENFDKDLPV
                     VEKIINSIKIIK"
     gene            7008..10424
                     /gene="ileS"
                     /locus_tag="VF222_10690"
     CDS             7008..10424
                     /gene="ileS"
                     /locus_tag="VF222_10690"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7142932.1"
                     /translation="MELHGQFDPKIIENEIRDYKKLIRYDEFFKKEIDIRHNKKIIGY
                     VDGPPTMNGEPHVGHLRGRIIKDLWFRFQTLKQNKVIFRPGWDTQGLPVELQAEKELG
                     LTGNKTENINKVGIEKIVETCKSIVKKYNKKWIEVDELLGMSFDYENGYWTFHDNYIE
                     REWSYLKTAWDNGILQEGFRVVAFCPSCQTSLSNSEVNQGYKTVEDPSFYYKVRLVNE
                     NAYLIVWTTMPFTLITDELVGVNPDANYVYVEAEKETWIVGETRLEELMNELHIEFYQ
                     IKKIIKGKDLDGKYYVHPLLDLIPELKKLADSKSIHFVVSEDFVDITTGSGIVHLSPA
                     NGEEDFNIAKKKNIPIFVPIDDRVVFTDQAGAFSGYFVRDTDEKIVQIMKDLKASIKI
                     GKIKHQYPTCWRSHHKLVWLARREYFYMIDKLGDKSIEAAKDVEYFFEAPKNRFIEII
                     KEKVPWCISRERIWGTPIPIWICSNCRHKELLSDKNDLVNRSSNLVRDKNSNLILKID
                     EEKDQYNEIVPVNNNNISLTANGHFELHRPWIDQIKINCSKCNSLMTREPYVLDTWHN
                     SGAAPFASFNDHDYKKLIPALFLTEGIDQTRGWSYSLLMQNVILKNESTAPFKSFLFQ
                     GHVLDEKGNKMSKSAGNVIEANLMLEKNPIDLIRFYFIWKSSPIEALNFSLKEMTSRP
                     YQILSTLYYLHIYLKQNSVLDNFDRSIHDLKWVIKNNLLETPEKWLISVLQNLIKNVN
                     LLFENCRFHEATKAIEEFIINNVSQTYIPLTRNYIWNDSVEEVNRRLTIFSILDYVLE
                     KIDIILHPVSPFLTEYLYLNCFSKIKNLEEIDSSKDSILLSKWPELETSLIDQGLENI
                     FEKIKIIISLANSARMKAKIKRRWPVTEMVICSILEKETIAQSNNNTESYYDQNQLDI
                     ELAKPDISEILKNQLNVQSYKVKQIRTANTVEKVLEMINANLPILSLISIIRKSVAPQ
                     VKSDIGKVISEFEKLDKVEVLHTLKTSKKIRLIYDKASSSFVDIYEKDLEISFSVKEG
                     YVYAEKENLMIFMSTNRDQNLITKGLVRDLARNLQQLRKEKSYNPTEILSTAYIADLE
                     NDEVSSLSLHRNDLTYLVRVQSVVLSEEKMEGIEYKNIEIDGREINISIN"
     gene            complement(10427..11218)
                     /locus_tag="VF222_10695"
     CDS             complement(10427..11218)
                     /locus_tag="VF222_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7142933.1"
                     /translation="MSLLKKENTIKIDAIVTSPPYNINKSYSKYNDKRDKNDYLSWLQ
                     KIANKSLQILKEDGSFFLNVGGRPADSMLPFEICSKFIGAGYKLQNTIHWIKSISIEV
                     DDIGKKNNIINTDSSIGHFKPIISQRFLSDLHEYIFHFTKKGDIAIDKLAVGVKYQDK
                     SNIGRWISAKTDKRDRGNIWFIPYNTIQEKRPHPAIFPEKLPYLCIKLHGIKKNTVIY
                     DPFMGIGTTALACINLNVEFLGTEIDNKYIEIANENIKLRYIKNK"
     gene            11451..11795
                     /locus_tag="VF222_10700"
     CDS             11451..11795
                     /locus_tag="VF222_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142934.1"
                     /translation="MSENNKFNFRYLKYRSLEDMFKIILSSSQSALGISSLLYFLNYK
                     NQNLMFIYNSSIGSSLIHYNILSEKPLTKFIQLNKQSGEYKYIDDIGNESHSIYIPIL
                     ELENTTLEFPIT"
     gene            12039..12740
                     /locus_tag="VF222_10705"
     CDS             12039..12740
                     /locus_tag="VF222_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142935.1"
                     /translation="MKFIRGHDPSFKFSSYFKLRNQSPDNSDASYLISHRLVDIGFIE
                     VTKGKFLSNVKNYKFTTYGLFYVFQNISIYPPQFLIKYQDNIILQTLIYPFFNEKTIK
                     IGTGRFYSQIVSYIEECCNVTLNAIDDIKSAEKEEDKVKIFHKMKFDLEWLIRRLCFK
                     LTIMYNETNLLSINPNLNDDIKVTIYEYENKMKIQLSKDSQFITLVKNLHKDFNEGYS
                     ELINLHNENSDDIDK"
     gene            complement(13016..13477)
                     /locus_tag="VF222_10710"
     CDS             complement(13016..13477)
                     /locus_tag="VF222_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7142936.1"
                     /translation="MSTQQAKHVPRKMLLLSFTSLALIYTIMLIGVYITSSHQGLSCP
                     DWPLCPNGLGIPSPEYFFEHIHRMLVIITSAFIFATAIYAYFNARSVSTTAILAAVIV
                     IIQIVLGMVMVYSKLHALIVASHLATGILLFAMLLLTFISSYRERKNSLVK"
     gene            13811..14224
                     /locus_tag="VF222_10715"
     CDS             13811..14224
                     /locus_tag="VF222_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142937.1"
                     /translation="MIAKSFEDLICDILLQSDITFSIRNSGGICEARLQENMKFKIRD
                     EWATIGDEISPWHIHININEVDNARFVREFNNSHNRESFSIRFFDSSQNLIFRANFSK
                     LYDLNGNLNHEKVLKFQYLYEKYGKKEILNFKLNN"
     gene            14631..15386
                     /locus_tag="VF222_10720"
     CDS             14631..15386
                     /locus_tag="VF222_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7142938.1"
                     /translation="MKTIVFTMFVVFSILSGLSIYTTHQNVDDAFGEINKSIFKNSHF
                     AMGNSFVSDRYDTINQDNNYNSIKYTNEPTIFNINYYQQPSFGYAEDRNNYLNDYAYN
                     NYYDYRNEFVAHLDPYPVITDHGYYYLDSQGIVYLSFDYSLNELSYRVFVEGMSYLEG
                     DDVEIIEIHLGIHGQNGPSILSLCNEKIKEGHCREGPGLSVEGVLEEKDLTGPLKGSS
                     FGELIGYFKSGQSYVYIQSRDHTEGEMRGQIYY"
     gene            16009..>16221
                     /locus_tag="VF222_10725"
     CDS             16009..>16221
                     /locus_tag="VF222_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142939.1"
                     /translation="MSKEFLEDIPKDKKLVVELGIGDGNLINSLSEKDQDYYFIGIEK
                     QESYFDKAKYNIKSQNVKILHTLFEDV"
BASE COUNT         5927 a         2286 c         2312 g         5696 t
ORIGIN      
        1 actactacta gtactagtaa tgatgatgat tctatttctt ctccagctct tactaccaat
       61 caagacgatc taaataaaac gattgaagga atgttggtga agatacagga aatagcagaa
      121 aaaatacaaa ataataccag taaaatgata gtagataatg aatttctcag tcaagaagaa
      181 gtggccgata tggtcttgac tctgtcagat gatactatta gtaaattaat caacggtaga
      241 attatcccca atgatagagt tttttatcct gtcaaaccta ttgtgggaac ctctatcgat
      301 gataataacc tacaaaattt aaaagaaaag gtagtagtta tcacaacaac ctcatcaaag
      361 gaaaaagatg tacagcgaat cactaatctc gcaaataaat tgaatgaaac tggtgtaaaa
      421 caagtaataa tacttagcaa aaactcttct gatgatacaa aattttataa agattttcat
      481 agtcattcat tggaccttgc agacgaagag aaagtaaaaa gaatctttaa tactgccaaa
      541 acaaaatttg gaagtattga ttctgttatt catattacag gcgaatatga ttatagtcga
      601 agtttatcat ctttgtccag agaggaatgg gatgtactag tagagaactt tatccatatt
      661 cctgcattga taacaaggga agcagtcaaa tttatgtcac cggctgacgc aattcaaaaa
      721 ccatctcaat ttaaagattc gaagggaata atagtaattc tgggccctga ttctccatat
      781 gggaagaaaa tatctggttt atcaagggcc agatctgagg tatttagagg tgctcttcga
      841 ccctatacgg ctacagtaaa tcaagaattg tctgatgtct taggatcgaa aatcaaatta
      901 aacttaattc tacctggaaa tattgaagga atcgaatcaa atatagaaaa tgttacaact
      961 tctattttaa atttaattgc tggatcttca ttaaataaaa acgaatcaat tttctatatt
     1021 gacgaaggaa agaaagaaaa ataattaaaa tgacttgata ctagtactac tgctactgcc
     1081 actattatta ttatcatctc gccatctctt tcattccatt cttttatata agaataaact
     1141 aatattagta agtaaaataa aagattaaat ctataaaaca aataacataa aataaataaa
     1201 aataattttt tgaataatga taataaatac agatacgatt atttcttcta tttctatttt
     1261 tatgttatca tcttaggaat gggtaaaaat tctattttta ttttattttc tgccttaaac
     1321 atcgcgtcat ctgtgatatc tactgcagcg ttgtttgcta ttttaaaatt gatcaaagat
     1381 cctcctaact gaccagcatt aaaaagaaca tcacataaca aatctgatat attcttaaac
     1441 tcatgatctg attccctaat atgggaaatt aataatgatc taatactttt cattactgtc
     1501 aatgacgggg tcagtttctc aactaaatct ttaaaactag agatattaca aatttgatta
     1561 tgcagagtat ctaaaacaat ttttgaagtc catatagctc tactcgattt tctaatacca
     1621 tttaattctg aagaaattaa tgaagaatat tgaatgttgt tctctcttat agaaatatct
     1681 aattttttta ttaattcttc atcttgtgtt tttaagtcat tgaatatatt ttgaagcttt
     1741 aaaatttcat cgctaattct gaattgtatt tgctttatct ttggtctcaa tggtatattg
     1801 tatttaatta aatgaaattc actatcatca ggtgaaaatt gagaactatg acaattctga
     1861 atactattat ttgaatatga attaaaatta ttggttttta cttgtttata ttcatctgtt
     1921 gctgttgctg ttgctgctgc tgctgctgct gctgctgctg ccgttgcttc tgctgacatc
     1981 actgttgctg ctgctgctct tgatccggct gttgtcgtta ttgttgttgt tgttaccatc
     2041 aaagaagaat tattctttag gatttctgaa ttatcatttt ctcccatgag actttctctc
     2101 ctttcttgta gtatgcatat ataataccat catgttcaat gtttggcagt aactatggta
     2161 acaccagtct aaagttacac aattcaattt agctcagaat tttttttgtt ttgacataat
     2221 ctagttgaca gagggatttt atttaaaatc ttatattaag tgaatattaa tgaatcaaag
     2281 acacattcat gacgatgact attccattca agatcccata attaataatt tagaaaacaa
     2341 taaaaaggtt tcaggaccag aaaaacagta tttttcacat gacgatagtc aatatagcaa
     2401 taataacaat ggtagtgatg gtgatactac taaatgtgtc agtaatatta gtattgaaaa
     2461 taataatgat ttaattggaa atttagaaga atttggtctc tcacaatatg aagctcgtgc
     2521 ctatttaact atgataggta aaggctctct ctcagctagt gaaatagcgt attattctca
     2581 tttaccaaga acaaaaattt atctcacctt aaaaaaatta gaaaaaaagg gcttatctgt
     2641 aatttctcaa caaaaacctc tgatatgtag tgcaataaat ccaaccgaag cttttgaaga
     2701 aattgtaatg atacaagaaa aacgattgat gaatatgaaa aaagctattg ataaattaca
     2761 aaagattaat gataaaagca attatttgaa aggtctagaa gaaaaaagat attttatatt
     2821 agatccacaa tctacctttc aaaaattatc taccctcata gaaaattctc gaaccagtat
     2881 tatagcaatt cttgacgttt ggggtttaag attgatttcg cagtgtaaat ctttattgat
     2941 taaagctatt accaagggaa taaagatcga atttttaatc tcaaatcaat gtatcggtag
     3001 tgaagattta tcttttttac caaatgaaat tttgataaag gttggaaatg ttttttctaa
     3061 tattattata ctggattcag gtaatataat aaatattgat gggggaaatg gtaaggcggc
     3121 attatttacc tccattgata tattaggatt ttcttatatg aaaatttttg aagaggaatg
     3181 gaaaaaagca attgagatca aaaatttaaa taaaactgaa tctttaaacc aattaacagc
     3241 cgttcaactc attagattta ttgaggacct ctcgcgcttt tccacatctc aatatttata
     3301 ttctatatat ctttcaattt tatcttcatt atctattgat aatagatctg atggttcagt
     3361 atcttcttct cagtcttctc ctcctcctcc tcctttttct cttactcacc attatccgac
     3421 gccgccttct tatcagagtc agcaaaaaga aggagaagaa aaagaggata gcgccacgac
     3481 gatattaagc aataaaaaag atataaatga acgaattttt gattataatt ctaaaattaa
     3541 ttttggtaaa aatcatcatc atgtttcatt tcttaattct ttggagagat atggtgtaaa
     3601 tctcactgat ttcactattg aggaacttat tcaaatagta gatttaattc taaagatgaa
     3661 ttattcagga gaattaaaat ttgacggcaa taataatatt ttaagtatcc attcgaaagg
     3721 caaaaacaaa tcattaatta tattgacaat attattgagt ttatatttta taaatttaaa
     3781 ctacgagatc gatattacac caaaaatatc agaaatatct gacgagatta ccattaatac
     3841 ctttcatatt aaatactcta aatcactcca ttagtattgt attatcatca tcatcatttt
     3901 tagttttcaa tttttaattt ctatctgcct gtaaaaatta tgaacatctc caaacaccac
     3961 taccaccatc ataatagctc tagttatttt ctatttgatc aaattttttc aacgcttctg
     4021 gaagattatc tagcagatcg cttgctacta tatgtaatcc tgacttttga tatgccataa
     4081 ttcccgcaat tccattaaaa taaacagctg tgcatgaagc atcaaacgaa tccaatttac
     4141 tataaaagcc tgcaaccaga ccagccaaga catcacctgt tccaccaact gtcatagcag
     4201 gtgtacttct ttcaataact gtagttagtt ctccatttga aataatattt ttccaaccct
     4261 tgagaataat tgttattcca tatttttttg cattgttaaa aacatctaaa atttgttcat
     4321 tttcactatt attattgtta gaatttttta attctgtttc aaaaattctc atatattctc
     4381 ccccatgagg ggtaataata caattcgagc cctttatttt gggcaatatc tcaggaatta
     4441 atgcagatgc atcaagtacc agtttggttc cattctttaa tagtttttca accaaactca
     4501 ataatgcttc ttttttagcg atactcatac ccataccaat tgttgctaca tctacttttt
     4561 ttggaagtgc tttaattaat cgtgctgccg aacccattgt taatctttga tcaactagag
     4621 gtattactat gagattaggt gaatatgatc gagttgattc tacattaaca ttcggaacag
     4681 ccgtatatac caaatctgta ccagaccgta aagcggctat tgatgccaat actggagctc
     4741 catgataaat cctacttcct cctacgacca gtacaattcc attatctccc tttctggacg
     4801 aattttttct tggcactaaa actcttttta tcatatcgtc atttattgtc actaattcag
     4861 gagattccaa tattttatta aaatgaccat aatcaataaa ccttacttgt aaacccttat
     4921 ttgatttgtt ttttgctatc tcactaatag aatagaacta ttctatttat tattgggtgc
     4981 tattttatca acccggaaca tttaaagaaa gaaaggaata gactatcttt tgtacaatgt
     5041 tttctatgtc aaaacctatc gagatagttt tttctgaaaa aaaaatgccc tctttaaatt
     5101 ccccacattt aatctgcgga tttcctggaa ctggattagt tggtaaatta gcagttgatt
     5161 atttgataaa ggaacttggt gctattcata tggctgatct cttttcatct tatttttcac
     5221 cacaggttgt aatacaggta gatggaacca ccaatatagt taaaaatagt ctttattatg
     5281 taaagaaaaa taataataat aataataatc ataataataa aacaagcaaa aacaaaaaag
     5341 aaattaaaga tattagtaaa cccgaagtac aatcagatca atcaattaat gatcctgctg
     5401 cttctccttc tcctcttcct caatcaaccg tctcattaaa cccgactaat actactgttt
     5461 cgccgttatc atctcaagat ctcattctcc tcactggtga ttcacagcca gtagttccag
     5521 gttcagaata tgtattatct gaacaaattt tagacttaat tacaaaattc aagatttcaa
     5581 atatctattc attagcatca tatgttactg gcacctttgt aaatgatccg aaaatttatg
     5641 gaacagctac taatccagaa atgataaaat cttttaggtc ctttaatatt tcaacccttg
     5701 acaatggaaa tattacagga atgaatggtc taatacttgg attgggaaaa cttagaggaa
     5761 tagaaggaat ctgtttgtta ggcgaaactt ctggatatgt aattgatgca aaagcatcaa
     5821 aaaatctttt agaaattttg aacaatgtac tgggaataaa tatcaatatg gatgaaatga
     5881 ataaaagatc taaagatacc gaaatactta ttaaaaattt agaacagcag atgatggcca
     5941 agtcgggaca atttccacaa ggtgttccta ctgaagaaca agaagaagaa ttaatgtttc
     6001 ctcaaaatag aaaatctaat atgggatata ttagttaaat tttttttagc taacataatt
     6061 taaaactaaa ttttctgtct aaatttattc agtttatgtg agattatcta atacattcta
     6121 atttgtaaat ttacaacaaa gattattatt aataatcttt gtttaacata atgtatgttc
     6181 aattacttta caagcttact gctattatct tcagtaattt tttttatcct tatttcattt
     6241 gatttagaat tcaccaacag ttctaaaatt tcttttggtc aaaactctaa tcttattgat
     6301 agtactaata ttagtagcag catcaataat agtaaaaatg aaacaccaac cacaacaaca
     6361 acaacagcag gagaagcagc aaccgataat tggctgactt attctaataa taaatttgaa
     6421 cttaaatatc cacctgattg ggatgtagaa aagaaattat caaaatttca tgtccttgat
     6481 tttaaactaa ttaaacataa ccccttctcc tttataggaa tctcgttttc atcacttcca
     6541 gattctgaaa agtttacaaa tgaaattatt ttagatgagg ccgaaaagtt tggtggtatg
     6601 tcaccccctt cttcttcttc tggatatgag ttttatcaag ttttagaaaa agaaccaaac
     6661 aaatacacga tagatgctaa tccttcatct tttcatattg taaaatataa atataaagat
     6721 aatgacctcg aaggtaaact tatggaaatt ttttctctca ttgataaaag attattcaca
     6781 ttaacttatc agtccacaat agaaaatttt gataaagact tacccgtggt tgaaaaaata
     6841 ataaattcta taaaaattat aaagtaataa taataaacca acatttctta ttttacaata
     6901 gcactttttt agtagctaaa ctatcttaac ttcttctcct tctccttctt atatcatcat
     6961 ccaaaaacat aaagaatggt tcgatagcat catcttctta tatatttatg gaacttcatg
     7021 gacaatttga tcccaagatc atcgaaaatg agatacgtga ctataagaaa ttaatcaggt
     7081 atgatgagtt ttttaaaaaa gaaatagata ttcgacataa taaaaaaatc atcggatacg
     7141 ttgatggtcc tccaaccatg aatggagaac cgcatgtagg tcacttaaga ggacgaataa
     7201 taaaagattt atggtttcga tttcagacgt taaaacagaa caaagtaata tttagacctg
     7261 gatgggatac ccaaggttta ccagtagagt tacaggcaga aaaagaatta ggattaacag
     7321 gaaataaaac agaaaatatt aacaaagtgg ggatagaaaa aattgttgaa acgtgtaaat
     7381 ccattgttaa aaaatacaac aaaaaatgga ttgaagtaga tgaattgctt ggaatgtctt
     7441 ttgattatga gaatggatat tggacttttc atgataacta tattgaaaga gaatggtcat
     7501 atcttaaaac tgcttgggat aatggaattt tacaggaagg atttagagtt gttgcatttt
     7561 gtccatcatg tcaaacgtct cttagtaatt ctgaagtgaa tcaaggatat aaaactgttg
     7621 aagatccgtc gttttattat aaggtacgat tagttaacga aaatgcatac ctgatagttt
     7681 ggactacgat gccatttaca ttaattacag atgagcttgt aggagtaaat cctgacgcta
     7741 actatgttta cgtagaggca gagaaagaaa catggattgt aggagaaact agattagagg
     7801 agctgatgaa tgaactccat atagaatttt atcaaattaa aaaaatcata aaaggtaaag
     7861 atctggatgg aaaatattat gtccatccat tattagactt aattccagaa cttaaaaagc
     7921 tcgcagattc aaaatcaatc cattttgtgg tgtctgaaga tttcgtagat ataacaacag
     7981 ggagtggaat agttcactta tctcctgcta atggagaaga agacttcaac attgcaaaaa
     8041 agaaaaatat tccaattttc gttccaattg atgatagagt agtctttaca gatcaagcag
     8101 gagcgtttag tggatatttt gttcgtgata ctgatgagaa aatagtacaa ataatgaaag
     8161 atttaaaggc tagtataaaa attggaaaaa ttaaacatca gtatccaaca tgttggcgtt
     8221 ctcatcataa acttgtatgg ttggcaagac gagaatattt ttacatgatc gataaattgg
     8281 gagataaatc tatcgaggct gcaaaagatg tagaatattt ttttgaagct cctaaaaacc
     8341 gatttatcga aataatcaaa gaaaaagtgc catggtgtat ctctcgagag cgtatttggg
     8401 gtactcctat tcctatttgg atttgttcca attgtagaca taaagaatta ctatcagata
     8461 aaaatgatct agtgaataga tcttctaatc ttgttagaga taaaaatagc aatttaattt
     8521 taaaaattga tgaagaaaaa gatcaatata atgaaattgt accagttaat aataataata
     8581 tttctcttac cgctaacgga catttcgaac tacataggcc ttggatagat cagataaaaa
     8641 taaactgttc caaatgtaat tctttaatga caagagaacc ttacgtactt gatacttggc
     8701 ataatagtgg tgctgctccc tttgcatcat ttaacgatca tgattataaa aaattgattc
     8761 ctgcattatt tttaactgaa ggtatagatc aaactagagg ttggtcgtat tctctattaa
     8821 tgcaaaatgt aatcttaaag aatgagtcta ctgcaccatt caaatctttt ctttttcaag
     8881 gacatgtatt agatgaaaaa ggtaataaaa tgagtaaaag tgctgggaat gtaatagaag
     8941 ccaatttaat gcttgaaaag aaccccatag atctcattag attctatttt atctggaaat
     9001 cgtcacctat agaagcattg aatttcagtc taaaagaaat gacttcaaga ccatatcaaa
     9061 tactgagtac actttactat ttgcatatct atctaaaaca aaatagcgtt ctcgacaatt
     9121 ttgatagaag tattcatgac ctcaaatggg taattaaaaa taacttatta gaaactcctg
     9181 aaaaatggct tatttcagta ttacaaaatt taataaaaaa tgttaatctg ttatttgaaa
     9241 attgcagatt tcatgaagca acgaaagcta ttgaagaatt tataataaat aatgtcagtc
     9301 aaacatacat accacttaca agaaattata tttggaacga tagtgttgag gaagtaaaca
     9361 gaaggcttac aattttttca attctagatt atgtactaga gaaaattgat attattcttc
     9421 atcctgtatc tccatttctt acagaatatc tctatttgaa ttgctttagt aaaataaaaa
     9481 atctagaaga gattgattcc tctaaagata gtattcttct ctctaaatgg cctgagttag
     9541 aaacatcttt aattgatcaa ggtcttgaga atatttttga aaaaataaaa ataataatct
     9601 ctttagcaaa ttctgcaaga atgaaagcaa aaattaagcg aagatggcct gtaacagaaa
     9661 tggtgatatg ttccatttta gagaaagaaa caatagcaca gtccaataat aatacggaaa
     9721 gctattacga tcaaaatcaa cttgatatcg agttagctaa acctgatata tctgagatat
     9781 tgaaaaacca attgaatgta caatcctaca aggtcaaaca aattcgtacc gcaaatacag
     9841 tagaaaaagt tctagaaatg attaatgcca atctccctat tttatcttta attagtatta
     9901 ttcggaaaag tgtagcacca caagttaagt ctgatatagg taaggttata tcagaatttg
     9961 aaaaacttga caaagtagag gttcttcata ctcttaaaac atctaaaaaa attcgcctga
    10021 tttatgataa agcttcctct tcttttgttg atatatatga gaaagatttg gaaatttcat
    10081 tttcagttaa ggaaggatat gtttatgcag aaaaagaaaa tttaatgata tttatgtcta
    10141 cgaatcgtga tcaaaactta attacaaagg gtctagtacg tgatttggct aggaacttgc
    10201 aacaattacg taaagaaaaa tcatataacc caactgaaat tctatcaact gcatatattg
    10261 ctgacctaga aaacgatgag gtatcatctt tatctttaca tagaaacgat ttaacatatt
    10321 tagttagagt ccagtctgta gttttatctg aagaaaagat ggaaggtatt gaatataaga
    10381 atattgaaat tgatggaaga gaaataaata tatcaataaa ctaggcttat ttatttttta
    10441 tatatctaag ttttatattt tcattggcaa tttctatata cttgttatct atctcagtgc
    10501 ctaaaaattc cacgtttaaa tttatacatg ccaaagccgt cgtacctatt cccataaaag
    10561 gatcataaat aactgtattt ttctttatac catgtaattt aatgcacaag tatggtagtt
    10621 tttcaggaaa gattgcagga tgaggtcttt tttcttgaat tgtattatat ggaataaacc
    10681 agatatttcc tctatctctc ttatctgttt tagctgatat ccatcttcct atatttgatt
    10741 tgtcctggta ttttactcct actgccaatt tgtcaattgc tatatcccct tttttggtaa
    10801 agtgaaatat gtattcatga agatcactta gaaacctctg actaattata ggtttaaaat
    10861 gtccaataga tgaatcagtg tttataatgt tatttttttt tcctatatca tccacttcga
    10921 tcgatattga ttttatccaa tggattgtat tttgtaattt atagccagct ccaataaatt
    10981 tactacaaat ttcaaaagga agcatagaat ctgcaggtct tccaccgaca ttgaggaaaa
    11041 atgatccatc ttcttttaaa atctgtaagc ttttgttagc tattttttgt aaccatgaaa
    11101 ggtagtcatt tttatctctt ttatcattat atttagaata tgacttatta atgttataag
    11161 gaggtgatgt tactattgca tcaattttaa tagtattttc tttttttaat aggctcataa
    11221 attccataca atcagtattg taaattttat ttacatgaat cattgatctt aattcgtgga
    11281 atgaaacaga ttgacatata agatttatgg atctgatttt gagagaatta ttataatcaa
    11341 taatccggaa tataagcact tataatttat aacaaactaa caaaaattga ttttcttctt
    11401 tattagattt attagcaagg tctaataatg ggtattattt attcattaac gtgtcagaaa
    11461 ataataaatt taattttaga tatcttaaat atcgttctct tgaagatatg tttaagataa
    11521 tattgtcctc atcccaatct gcgttgggta ttagctcatt actttatttt ctaaattata
    11581 agaaccaaaa tcttatgttt atctataata gttcaatagg aagcagttta atacattata
    11641 atatattgtc tgaaaaaccc ttaaccaagt ttattcagtt gaataagcaa agtggagaat
    11701 ataaatatat agatgatatt ggaaatgaat ctcattctat atatattcca attctggaat
    11761 tagaaaatac aacattggaa tttccaatta cttaattaat taattattct attaccgaga
    11821 cacatataat tttgtattgg ttgttgtttt ataaatttac acatgaataa attaaattta
    11881 agataagcat aacttttgaa tataaatttt taataaattt atataattta taaatctgta
    11941 atacaattac tatactaaac atggctcact cagattatat atcttcctta ttgtcttatc
    12001 caaaaatcaa tttaactcaa gaggaacgat catttataat gaaatttatt cgaggtcatg
    12061 atccatcctt taaattttca tcttatttta aattaagaaa tcaatctccc gataatagtg
    12121 atgcctcata tttgatttca caccgtttag tagatattgg atttatagaa gttacaaaag
    12181 gcaagtttct gagtaatgtg aagaattata aatttactac ctatggattg ttttatgtat
    12241 tccaaaatat ctccatttat cctccacaat ttttaataaa atatcaagat aatataattt
    12301 tgcaaacctt gatataccca ttctttaatg aaaaaaccat caagataggt actggaagat
    12361 tttattctca aattgttagt tatatagaag aatgttgtaa tgtaacctta aatgctatag
    12421 atgatattaa atcagctgaa aaagaagaag ataaagtcaa aatttttcat aaaatgaagt
    12481 ttgatttaga atggctaata cgcagattat gttttaaatt aacaattatg tataatgaaa
    12541 ccaatttact atcaattaat ccaaatctaa atgatgatat caaagttaca atttacgaat
    12601 atgaaaacaa aatgaaaata caattatcta aggactcaca atttattacc ttagtaaaga
    12661 atttacataa agactttaac gaaggctaca gtgaacttat aaatttacat aatgaaaatt
    12721 ctgacgatat agataagtaa tattaactat gatagcttat tttagtatta tatagtcctt
    12781 tttcactttt acgttccttt atctgaacaa cattaatttg taataaacct aacaccaata
    12841 tttttacagg atgccttagt atcttgtgag tgtctatcta tttttttata ttagttattc
    12901 agaattcaaa aatctttatt tttgttatta ctgatagcat tttatataat acccaagaaa
    12961 tcataatatt tattctttaa ctaatttcta tacaaaatat ttctatttgt atacgctatt
    13021 taactaatga attttttctt tctctatatg atgaaataaa agttaataat aacattgcaa
    13081 ataataatat tccagtcgcc agatgagatg ctactatcaa tgcgtgcaat tttgaataca
    13141 ccatgaccat acccaaaact atctgaataa taactatgac ggctgctaag attgcagttg
    13201 tactaactga tcgtgcgtta aaatatgcat aaatagctgt tgcaaaaata aaagcacttg
    13261 taattatgac caacattcga tgtatatgtt caaaaaaata ttctggactt ggaataccta
    13321 aaccattagg acataatggc caatcaggac aggacaatcc ttggtgagat gaggtaatat
    13381 aaactcctat caacattatt gtatatatta aagctagtga tgtaaaagat aacaatagca
    13441 tttttctggg aacgtgtttg gcctgctgcg tagacatggt agtttactct aaatattttt
    13501 cttgacccaa ataaatgtaa atctattatt atttttgtaa ttttttattt tattagttat
    13561 cgtagtatgc taagatgtta attgtcgatt cctagacttt ctatagatat tgcgaaactc
    13621 taaaaataca gtatatatac atcatttaga aagaaatgac tttttatggt ctatataata
    13681 tgtcgacttt ttaattagga gatttcgtta aataaacttt atttattgca tgtatctttt
    13741 gcaaagctgt gatatgatat taatagattt tcaaattatg tttattaatg gatttgatta
    13801 tctgtgatct gtgattgcta aatcctttga agatctaatt tgtgatatac ttctgcaatc
    13861 tgatataact ttttctataa gaaattctgg tggaatttgt gaagcacgat tacaagaaaa
    13921 catgaaattc aaaattagag atgagtgggc aactataggc gatgaaattt ctccatggca
    13981 tattcatatt aatataaatg aagtcgacaa cgcaagattt gtaagagaat tcaacaattc
    14041 tcataatcgt gaaagtttta gtattcgctt ttttgattca tctcaaaacc taatatttcg
    14101 tgctaatttt tcaaaactct atgacctaaa tggtaatctt aaccacgaaa aggtgttaaa
    14161 atttcagtat ttatatgaga aatatggcaa aaaagagata ttaaatttta aactcaataa
    14221 ctaaattaaa ttattttgaa ataactaatt atctaaagaa tagtatctaa gccactttga
    14281 attatttatt aaaatccatt gttaattttt atgttattaa gattatctga gtatataata
    14341 tataaattaa ggtaataaat tgaatactaa atattaaatt attgattgct gtattatcag
    14401 ttaaaatgaa ttgattctta taaaaaggat attcactaat aataatttaa tagaaaattc
    14461 ttattaaagg tctataatat ttttcattta caaaaattct tttcttttaa tcaaatttaa
    14521 ttatttatgt taatatatta caatattagg tatgtaaatc ctactatagc atatagtaca
    14581 tgatctttct aattcttaaa atatttagaa ttgaattaat aagtaagaat ttgaaaacaa
    14641 tagtttttac tatgttcgtt gtattttcaa ttttatcagg gttgtccata tacactacac
    14701 atcaaaatgt tgatgatgcc tttggagaaa taaataaatc tattttcaag aactcacatt
    14761 ttgcaatggg taattctttt gtctcagata ggtatgatac tattaatcaa gacaataatt
    14821 acaattctat taaatatact aatgaaccta ctatatttaa tattaattat tatcaacaac
    14881 cgtcatttgg ttatgcagag gatagaaata attatttaaa tgattatgca tataacaatt
    14941 attatgatta tagaaacgaa tttgttgccc acttagaccc atatccagtt ataactgatc
    15001 atggatacta ttacttggat tcacagggta tagtgtatct gtcatttgac tattccttga
    15061 atgaattatc atatagagta tttgtggaag ggatgagtta tctagaaggc gatgatgtgg
    15121 aaataatcga gatacatctg gggatacatg gtcaaaacgg acccagtatt ttatcactat
    15181 gcaatgaaaa aataaaagaa ggacattgta gagaaggacc agggttatca gttgaaggtg
    15241 tattagagga aaaagatttg acaggtccgt taaaaggctc gtcatttggt gaattaattg
    15301 gatactttaa atctggtcag tcttatgtat atattcaaag tcgtgatcat actgaaggtg
    15361 aaatgagagg gcaaatatat tattaattaa aatatttgtg tatatatcaa gaatctctaa
    15421 agttagatcc aggcaataaa tttatctatt gttacagtaa aatattagat ttttaaaatg
    15481 agtttaataa ttatagttta catgagcttg gaactaatca attcaattgt aaattgttta
    15541 tttataatat ccaattaacg atatctttgt ataaaaaagt taattgataa gagtattttc
    15601 aaagttatta tattaacttg gttgaaataa aacaatatca ataatcccgg tatctctgta
    15661 atagaactga ctatatgtaa tatatatata aaattattta atttatattg ggttcttaaa
    15721 tttttttctg ccaataccta atttatctaa atctgcaatt taacaatttt aactttattt
    15781 atcgtaccaa gatcgggtat taaaataata tatatagata gagagattta ttactacaat
    15841 tataaactgt gactatacat ctatataaaa attaaaaaaa attttaattg tttgtataat
    15901 ttttttgtag tatattgcat gactaaaaat gtattttgaa attaattaaa taatttacaa
    15961 tattttaata aaatacaaat aatctattat aaaatcatga ttatgccaat gtctaaagaa
    16021 ttcctggagg atattccaaa ggataaaaaa ttagttgtag agttgggaat cggtgatggg
    16081 aatttaataa attcactctc agaaaaggat caggattatt attttattgg catagaaaaa
    16141 caagagagtt attttgataa agctaaatat aatattaaat cgcaaaatgt taagatactt
    16201 catactttat ttgaggacgt t
//
LOCUS       DASVEV010000247         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_704121, whole genome shotgun sequence.
ACCESSION   DASVEV010000247 DASVEV010000000
VERSION     DASVEV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704121"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF222_10730"
     CDS             complement(<1..949)
                     /locus_tag="VF222_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7142940.1"
                     /translation="MPSDEIENVLILQGGGSLGAFACGVFKALVKNNIKIDIVAGTSI
                     GGVNASIIAGSKQKDSPETALEQFWLELAEGDINSHSYPFTNWLLYGLTNPLTSVADP
                     TRNIHGSQIKSLSSFYKSAVFGNNKVFIPRWNPEYAFNDPEYFNPNKWTYLYDHSPLI
                     KTLEKYVDFNKLQPNGNPNSRLIITAVNVLTAEPLAFDSSSQQITPKHILAATAYPKY
                     YFPWVEVEEGVYAWDGSLLSNTPLREVIDASPVKNKRLFLVENYPKDIDNLPANLFEV
                     EHRTRDILFSDKTMQNLKMSKAFTVYLKLIDDLYNMLENN"
     gene            1007..1159
                     /locus_tag="VF222_10735"
     CDS             1007..1159
                     /locus_tag="VF222_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142941.1"
                     /translation="MNSNTTNDNLNLALEYIMINNEIWEINYNEGHAIEASGYKKREF
                     LEKKYN"
     gene            1412..1636
                     /locus_tag="VF222_10740"
     CDS             1412..1636
                     /locus_tag="VF222_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7142942.1"
                     /translation="MIEYIKILETMNNSKIARLATVDPENNQPYLIPVVFVSNGTNIF
                     IPIDDKPKKKVKSKQLKRIKIYKKILTFLF"
     gene            complement(1674..>3226)
                     /locus_tag="VF222_10745"
     CDS             complement(1674..>3226)
                     /locus_tag="VF222_10745"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7142943.1"
                     /translation="DITLISKDNFFLFTPMLHEVSSGMIETRHVASPLRAFCKRARFI
                     ESEVKSVDLENKTIIFTNPVLDARLRYSIGEEQVMKLDTCLLSNDHLASKSPLRSLTN
                     NTKNYDNNYENINKDHYIKSLDYDYLVLALGSNTNFFGNKNLEKASFTMKNLYDAFSI
                     RSHIIDTLEQTDMLLFEENKDVVIDQEIKNSTIKISYSNYRDNENQLENLRKSLTTYV
                     VVGGGFAGVETIGEINDFIKESIRDYYQNIDIKDIRVILINSGSRILPEMDEQLGRFA
                     LEELKKKKIEVILNNRVVDVKDIVEDNKNKENLLNQHGPKKIILKDNQEIIADTLIWT
                     AGVVPEKSVTDIYSCEHDKSGRIVTDKYLQIKGFKDVYAIGDCAYIIDPNTEKPCPPT
                     AQHAIREGYVAGENIISSIKKELKFAKTIDMKENEKRKEIIEYDKKEFSYKTRGIMAT
                     IGKRNGVAMIFGHSINGIFAWGIWRFFYLTHLPTLENKIRVMIDWTVDLIFGTNLTRL
                     KSPVDTKI"
BASE COUNT         1079 a          517 c          417 g         1213 t
ORIGIN      
        1 aattattttc aagcatatta taaagatcat caattagctt caaataaacg gtaaaagctt
       61 ttgacatttt tagattttgc attgtcttat cactaaacaa aatgtcacgt gttctgtgct
      121 ccacctcaaa caaattcgca ggtaaattat caatatcttt tggataattt tcaactagaa
      181 agagccgctt attctttacg ggtgaagcat caatgacttc tcttagtggg gtattactaa
      241 gcaaactgcc atcccatgcg tagacacctt cctctacttc tacccatgga aaatagtatt
      301 taggataagc agttgcagca aggatatgtt ttggagttat ttgttgacta gaactatcaa
      361 atgctaatgg ttctgctgtc aagacattaa ctgcagttat tattaaacgg ctattaggat
      421 taccattggg ttgtagtttg ttaaaatcaa catatttttc tagagttttt attagtggag
      481 aatggtcata taaatatgtc catttattag gattaaaata ttctggatcg ttgaaagcat
      541 attctggatt ccagcgtggt ataaagacct tattatttcc aaaaactgcc gatttataga
      601 atgatgaaag agacttgatc tgtgaaccat gaatatttct tgtaggatct gctaccgaag
      661 ttaatggatt tgttaatcca tataataacc aattcgtaaa agggtaggaa tgagagttta
      721 tatcgccctc tgcgagttct aaccagaatt gttctaatgc cgtttcagga ctatctttct
      781 gtttacttcc agctattatg gaagcattta ctccgccaat tgatgtccca gcaactatat
      841 ctatttttat attatttttt acaagtgctt taaaaacacc acatgcaaaa gcacctaaag
      901 aaccgccacc ttgaaggatt agaacatttt cgatctcatc tgagggcata tcaaatataa
      961 cattgattat aataatttat agctaattga atatgcagtt tatattatga attctaatac
     1021 aactaatgac aatctaaatc ttgcactaga atatattatg ataaacaatg agatatggga
     1081 aataaattat aacgaaggcc atgctatcga agcaagtgga tataaaaaaa gagaatttct
     1141 tgaaaaaaaa tataattaaa tactctaatg atactattga agatttttta aaccaaagaa
     1201 agaggaagca gagctattca ctttcacaat acgatgactt tacctcgtat acatttgaaa
     1261 taatatagaa aggatagaaa acaatatgca cttttagaaa agtgtaagta aataaagtca
     1321 cttcctagtt ttaatgtata tacaaatatc tcttctgtta tcgccaaata aattttttta
     1381 ttaactatta tatttatttt ataatagtga tttgatagaa tatataaaaa tattagaaac
     1441 tatgaataat tcaaagatag ctagattagc aaccgtagat cctgagaata atcaaccgta
     1501 tctcatacca gtagtgtttg tttctaacgg tactaatatt ttcattccaa tagatgacaa
     1561 acctaaaaaa aaagtaaaat caaaacaatt gaaaagaata aaaatataca aaaaaatcct
     1621 aacatttctt ttctaattga tacatatgta tgtactaata ttcttctttg atatcatatc
     1681 tttgtgtcaa caggtgactt tagtcttgtt aaatttgttc caaaaataag atctactgtc
     1741 cagtctatca taactcttat tttattttct aaagttggta aatgagtcaa ataaaaaaat
     1801 ctccatatcc cccaagcaaa tattccattt attgaatgtc caaaaatcat ggctactcca
     1861 tttctttttc ctattgtggc cattattcct ctagttttat atgaaaattc ttttttgtca
     1921 tattctatta tttcctttct tttctcattt tccttcatat caatagtctt tgcaaatttt
     1981 aactcttttt ttattgatga aattatattt tctcctgcaa catatccttc acgtatagca
     2041 tgttgagcag ttggaggaca tggcttttct gtatttgggt ctattatgta tgcgcaatca
     2101 cctatagcat agacatcttt aaaccctttt atctgcaaat atttgtcagt tactattcta
     2161 ccacttttat catgttcaca tgaataaata tcagtaactg atttttcagg aacaactcct
     2221 gcagtccaga taagtgtatc tgctattatc tcttggttat cttttagtat tatttttttt
     2281 ggaccatgct gatttaatag attttcttta tttttgttat cttctactat atcttttaca
     2341 tcaacaactc tattattcaa aatgacttct atcttctttt tctttaattc ttctaaagca
     2401 aagcgaccaa gctgttcgtc catttctggt aagattctag aaccagaatt aattagaata
     2461 actctaatat cttttatatc tatattttgg taataatctc taatagattc ttttataaaa
     2521 tcattaattt ctccaattgt ttcaacacct gcaaaacctc ctcccactac aacatatgta
     2581 gtcagactct ttctcaaatt ttctaattga ttctcattat ctcgataatt agaataagat
     2641 attttaattg tactattttt aatttcttga tctattacta catctttatt ttcttcaaat
     2701 agtaacatat ctgtttgttc taaagtatca ataatatgac ttctaataga aaatgcatca
     2761 taaagatttt tcattgtaaa tgatgctttt tccaagtttt tatttccaaa gaaatttgtg
     2821 ttacttccca atgccaaaac tagatagtca taatctaatg atttgatata atgatctttg
     2881 tttatatttt cataattatt atcgtagttt tttgtattat ttgtcaaact tcttaaagga
     2941 ctttttgaag ctaaatgatc attagataat aaacaagtat ctaatttcat aacttgttct
     3001 tctcctatac tataacgaag tcttgcatct aaaactggat tagtgaatat aattgtttta
     3061 ttctctaaat ctacagattt aacttcagat tctataaatc gggctctttt acaaaatgct
     3121 ctcaatggtg atgctacatg tcttgtctca atcattccag aagatacttc atgtaacatg
     3181 ggagtgaaca aaaagaaatt atctttgcta attagagtta tatcta
//
LOCUS       DASVEV010000248        13482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_514785, whole genome shotgun sequence.
ACCESSION   DASVEV010000248 DASVEV010000000
VERSION     DASVEV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514785"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            713..838
                     /locus_tag="VF222_10750"
     CDS             713..838
                     /locus_tag="VF222_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142944.1"
                     /translation="MDCSRRFAFPITKKVLLVGVEDEAANIFRVLKLPATKRVQR"
     gene            complement(816..959)
                     /locus_tag="VF222_10755"
     CDS             complement(816..959)
                     /locus_tag="VF222_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142945.1"
                     /translation="MRYEAARLASLKDVDLSKIVSHRYSLSNIEESMIASEFTVVIVG
                     LVL"
     gene            complement(1083..1301)
                     /locus_tag="VF222_10760"
     CDS             complement(1083..1301)
                     /locus_tag="VF222_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142946.1"
                     /translation="MNAEEAKALLKDRGVSVINMPRGYMVTVSRREGTPSRRIEMDEM
                     DAILALGKIRLLAVEDTVRDILIEVSKT"
     gene            1852..2019
                     /locus_tag="VF222_10765"
     CDS             1852..2019
                     /locus_tag="VF222_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142947.1"
                     /translation="MDSSFRTDIGIISSLRHSIEFNIMGIYLKIVLIIKAKYKIVVEP
                     YYVDGSTPLST"
     gene            2098..2913
                     /locus_tag="VF222_10770"
     CDS             2098..2913
                     /locus_tag="VF222_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7142948.1"
                     /translation="MPFIESNNKDKLKIYYEVYGKDKPDTVVLVHPIGGNIDIWNEEI
                     SLISKEKNLRIIAYEIRGHNRSNMGTTNSFTISDLVEDLKILLDSLDIKKCTLIGHSI
                     GGSIASLYAKQYPQKVEAIIFINASSVRIPDKDLEKHFTTRKIAVTQGMDALAEWARH
                     ETLESEEAFKNDDNWNNFKQIFTKTSVEGFVAATNSLYSMPDDVTDSLKDAKIFGIVG
                     EGDEVFMRLMHKMKEEIPRFELRIIEDSDHWVIVEHPKELDKALIEFLNKIYP"
     gene            complement(3547..4602)
                     /locus_tag="VF222_10775"
     CDS             complement(3547..4602)
                     /locus_tag="VF222_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142949.1"
                     /translation="MPSIRFTSIIFSIFPLLFVNTFIFIPIFGDGLFQEELSASMAGR
                     DLSLLIKMSPPVVTTDTLKGGQTPLIQFRLSDTATNETLKHVTYLIEIEKSGKRLLLD
                     TFHSHTGNLGIQMRPSSADHITINGEQDTILLAYTGDMEHPVSATGPIFAEGGLYHFI
                     VTIETLDFDRTFIPTDNQPVYEGWLSVGYSIDKDLEINGTAYTIDAISYYDALNNFNY
                     DSSANELKFAMPFDWNLDRLKKVNLYVHEEISIPKPSPLAANGYKGTVNNIDLTNKSL
                     LVDRTNSSKDVVHIMLTKPTVMDIAQKIVENNSNIQNNQSKTTEMTFTLFPSKNTITG
                     NMNMNSSSMNMPMNGGM"
     gene            complement(4897..6198)
                     /locus_tag="VF222_10780"
     CDS             complement(4897..6198)
                     /locus_tag="VF222_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142950.1"
                     /translation="MSFFKPSLNVSQFMLFLTISFIVLTIFCINNNSFAIENQIIKNE
                     NEFNIIATADVGCSIRAQENIKNIEKLDPELFLVAGDLSYKKTPDCWFNMTKSLDSKT
                     KIAIGNHDDYEEEGAKGERLERSLLNHYNLNKPYYSFDYQNVHVLVLDTQLELSVDTL
                     ESTAIINETTTTTNTIDDNKGKKDNKDANDKKKEPLLERFPLIDIADLLEQNSIDVEI
                     PALDKIIESNAKVPALKVDNEQYQFVLEDLEKTSQNKSIDWIFVMFHKPMYSPLSKQF
                     EEYIIRDKYQPIFDKYGVDLVIQGHNHIYSRTLPLSLNKLDISQPIVDKNSSSTSNNN
                     IFTNPQGTIFLVVGVGGDELHRIESVPYYVANHYNKGFGFADIKIDGKRLDVKFYDIN
                     LNCQLKITQKKEQEVIDPQSCLPPTPGNDILKVIDYFTIIK"
     gene            6424..8064
                     /locus_tag="VF222_10785"
     CDS             6424..8064
                     /locus_tag="VF222_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142951.1"
                     /translation="MINKNRTVFILLLSFPLLLFTFCNIYAQQNQPLVAENITLFDPK
                     LKVELVTSELDFPTTMAFLGPDDFLILEKNTGNVKRFVNGTVLEKPLLHVKPSIKDER
                     GLLGIAVSEKKEIFYENTFLIENKKLTHNVFLYYIECQKKNTDCENRIYKYDLDNQKN
                     VLVNPKLLVGVPSFPDPSHIGGILDIGPDENLYFTVGNFQNTIPSKIYKTKTQNVEDG
                     EPVDGRAGILRITQGGKPVGAEGIIGNEYPLNLYYAYGVKNSFGIDFDPVTGKLWDTE
                     NGPQFGDEINLVESGFNSGADKLYGIWESNEFGDILKDKKGESIVVGNNPANLVNFDG
                     KGHYSSPEFIWVKTIAPTALLFLDSDKLGSNYKNDMFVGSADGGRLFYFDLNDKRDGL
                     VLKGNLTDKIAYDKSEFNDILFAEGFSIITDLKQGPDGYLYIISGLKQSKTAKFGAVF
                     RIVPSGKSTTNPDKLDIVKQQTQEEKSKIISSITNTNTTTNTDLLSNQEDSPPKKTNT
                     NVLVDIAPKPSNTTKTIDVKAWDNLIRQNLNKPHIQSK"
     gene            8510..9691
                     /locus_tag="VF222_10790"
     CDS             8510..9691
                     /locus_tag="VF222_10790"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7142952.1"
                     /translation="MNLAIDTKRLFVIFSSISLSLLLAAVLTTGTVNAFENAIEYKFV
                     KKWGGAGSADGQFLRPHDLDFNKEETVLYIVDRDGSRIQAFDKDGKFLFKFGKDGTGD
                     GQMHVPYGIDVDKKGNIWLADRANDRAQEFDSKGKFVLKFGNVDGVPSAEEGKFDNPR
                     HVAVSKDLRYVYVADSKNNRISQFDTKGNYIKSIGQLGTKPGDFNLPTTIEIDSKGNF
                     FVNERGNERIQKFDSNWKPILMWGSKGSGDNQFCHIEHIALDKYDNVYVTDPQSDPGC
                     SQQPRVLKFDSEGKFITKWGSFGKGDGQFQDPEHLAVDSEGNVYISDRKRDDIQKFTP
                     ISETSNQSVNMSNTKLSTKVETPKKTNTNVLVDIAPKPSNTTKTIDVKAWDNLMQQKL
                     K"
     gene            9800..11158
                     /locus_tag="VF222_10795"
     CDS             9800..11158
                     /locus_tag="VF222_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7142953.1"
                     /translation="MNKKRITIFALSFPLLLFTFCNIYAQQNQPNGDESIVLSDPKLK
                     IELVASGLDFPTTMAFLGPDDFLILEKAGTVKRVTNGVVLDKPILQVDVSVKDERGLL
                     GIAISEKKNFGNDNGTSIKNNNISHNVFLYYVVCKEKNTGCENTIYRYDLDNKNNVLI
                     NPKLLLSIPSFPDPAHIGGIIDIGPDNNIYVTVGNFQHTIPSEVYRTKTQNYEDGEAV
                     DGRAGVLRITQDGKPAGEGILGTEYPLNLYYAYGVKNSFGIDFDPVTEKLWLTENGPK
                     FGDEINLAEPGFNSGSDKIFGIWRVNEEGKKLQTDGKEGDGEYVTVGNNPKDLVYFEG
                     KGHYSPPEFIWDKSTPPTALLFLDSKNFGTEYENDMFVGSVDGQIFHFDLNDNRTGLL
                     LKGVLKDKIAADDTEFADILFAKGFSIITDLKQGPDGNLYVVSGLKQSKSEKLGAVYR
                     IK"
     gene            11395..11715
                     /locus_tag="VF222_10800"
     CDS             11395..11715
                     /locus_tag="VF222_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142954.1"
                     /translation="MKPNKGNITYLCLVIVIIFVTTTLLVSSNDLSFAIKENENKDDS
                     QDNKKDVPISSTNTTKEILNLKLKSDVRNDIEDAHYPNQFYTCGYPQQLITDYNSFAK
                     FDCQ"
     gene            complement(12129..13139)
                     /locus_tag="VF222_10805"
     CDS             complement(12129..13139)
                     /locus_tag="VF222_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7142955.1"
                     /translation="MSNSTRVKIRLSIQAAHEQFNPNELLDDAIYLDKKEIERCWSSD
                     HYMPWWNTGASGGAAWPWMGAALAKTTKLVIGTGVTAPILRYNPAIVAQVFATLSYMF
                     PNRVFLGVGRGEALNEVPTGNIWPSNKEKFKRLREAIKLIKKLWTEDWVNFKGEFYSV
                     KDSNLYTKPDYLIPLYIAGLGEQSAQLAGEEGNGFVTNELDINKIKDKLLPAVRKGTS
                     KAGKNYDQLEKILFIPASYDEDKQKAIDSIRFWRGSMIKAFFDVDVHDPRKIEENGQV
                     IGDDSLEKKLFIITNEEEGIKKLQKYIDIGFTEIVFTNSSPNRKKFVDLVKKMSTTIN
                     SR"
BASE COUNT         4856 a         1936 c         2117 g         4573 t
ORIGIN      
        1 acagctgatt atattatttt aaatgattta ggttgggaaa tctatcctat tggattatac
       61 aatatgggta taaaaattaa caaatgtaca aatgttccca tcattataac tgaaaatggt
      121 atagcagata aaactgacaa aaatcaggtc cgtttattat agcacatctt gaacaagtaa
      181 aaagagcaat taatgatgga ataaatatta taggttatct acattggtct ttaatagaca
      241 actatgaatg gcaggaagga tataatccag aaggaaaatt tgggttatta tatattgatc
      301 acaaaaattc tgatttcaaa agatcaataa caaaggcggc aaaaatatat aactttatta
      361 taaaagaatc attaaaaact aacgaacaaa ttaatacttc cgcaataata gatgctagga
      421 aaattttggg agtctaagat ctgttaagat ccttctacaa atatgttatt gttaatctaa
      481 tatttagaaa taaaggattt aatacattcc tgcttttgtt ataaacatgg accaaaaata
      541 ttgtgtcaaa aacaattcac acacctctga gcccgatgaa tatatgtatt catataattg
      601 aaaaagaaaa tcaattttgt aacaatagcg tccaccgaca gagcattagg tatgttaatt
      661 atctgggtac tgaaaagtta gttcgtgttc tctaaacctt ctcacggctg taatggattg
      721 ttcacggaga tttgcatttc caattactaa gaaggtgtta ttagttgggg tagaggatga
      781 ggcagcaaat atctttagag tactaaaatt acctgctaca aaacgagtcc aacgatgact
      841 actgtgaact cactagctat cattgattct tcaatgttgc taagtgaata tctatgactg
      901 actatttttg ataaatcaac atcctttaaa gaagcaagtc tagctgcttc gtaacgcata
      961 ggtggcgtag agctgaaatt accagaacta actgtataag aaagcaagaa gggattaacc
     1021 tacgtaggta cgtacatagt cttatcatac attccaacta tctctgatgt tgtggcatta
     1081 gtttaggtct ttgatacttc gataaggatg tctctaaccg tgtcctccac agcaagcagt
     1141 ctaatcttgc ctaatgctag gatcgcatcc atttcatcca tttcgatacg gcgtgacgga
     1201 gtgccttccc ttctggatac tgttaccata tagccacgag gcatattaat aacggatact
     1261 cctctatcct tcaacaatgc cttggcttct tcagcattca tttttcaact gtacatgacc
     1321 gttttcgctt aaaaacacta cggacttgaa aaatgacggt attctatgct attccgaaaa
     1381 atatcgataa agtatttgaa tccaatctgt tgaatatttt aggaaggata aattgaattc
     1441 tgtctagact caattggaat ataacaccat agtaaaaggt cattaatttc aatctagata
     1501 tagccagatt cactgcagtt gtttgtttaa aggacttgtc tttggataca aaaagaaaaa
     1561 tatatttgac aatgatataa ctcctcgtcc tacttccttt attctttatt ctttctctat
     1621 cttcttctgg gacaaatagt tacattgtaa ggacagctct aaatcgtgcc ttaccactca
     1681 tcattagctc ataggcctca taggcacgtt cgagtggaaa tatttggttc atagatctta
     1741 caccggaaag ttcactaaag gagagagtat cttgtgaatc tatcgataca aaaataatta
     1801 agataagttt ctaataataa aagctaaata catgatacta ctatctctac attggactcc
     1861 tcttttagaa ccgatatcgg tattatttcc tccttgagac attctatcga attcaatata
     1921 atgggaatat atctaaaaat agttctaata ataaaagcta aatacaagat tgtagtagaa
     1981 ccctactatg tagacggttc cactccatta tctacttaaa atatatttta aaatgaattt
     2041 gtatataaat atggtattta ttacgataat aaattaatta attaagagaa ttaaaaattg
     2101 ccatttatag aatctaataa caaagataaa ttaaaaattt actatgaggt atatggtaag
     2161 gacaaaccag ataccgtagt tttagttcat cctattggag gtaatattga tatttggaat
     2221 gaagaaattt ctttaatttc aaaggaaaaa aacctaagaa taattgcata tgaaataaga
     2281 ggtcacaata gaagtaatat gggaactaca aatagtttta caatatctga tcttgtagaa
     2341 gaccttaaaa tattacttga tagtttagat attaaaaaat gtactttaat aggacactct
     2401 ataggtggtt ctattgcatc tttatatgca aagcaatatc cacaaaaagt agaagcaata
     2461 atatttatca atgcatcttc agttagaata cctgataaag atttagaaaa acactttact
     2521 acaaggaaaa ttgcagtaac tcaaggtatg gatgcgctgg cagaatgggc aagacatgaa
     2581 acattagaat cggaggaagc ctttaaaaat gacgataact ggaataattt taaacaaata
     2641 tttacaaaaa catcggttga aggatttgtt gctgctacca attctttgta ctcaatgcca
     2701 gatgatgtaa ctgattcatt aaaagatgct aaaatatttg gtatagtagg agaaggtgat
     2761 gaggtcttta tgagattaat gcataaaatg aaagaagaaa taccaaggtt tgaacttagg
     2821 attattgaag atagtgatca ttgggtcatt gttgaacatc ccaaagaatt agataaagca
     2881 cttattgaat tcttaaataa aatatatcca taatataatt tttaaaatta agattgctta
     2941 acttacaatt attttgaaat aacaatgatt gataatttgc aaatataaaa ataggttaaa
     3001 ggaatgggta tgaaaaaaat taggataata agaaaagatt ctaacaaaga gattatactt
     3061 ttgttagcag cgataacatt ctaaataata caaaaaataa tgaatctgca ataacaacac
     3121 ataatgaaaa aaagccagcg aataaacctg ataagagaat tgaaaaaaag attcattcaa
     3181 agaaagtaga caataaaggc agaaagattt taagaaaaag tttgcaaggt cattcacccg
     3241 tatatgaata atcacattgc agaatataag ttaccaaaaa gctaaatact atgtagtaga
     3301 atcctactca gtaaccaatt ccaaatgaaa aaataacttc tttcggtaaa aggttgtaat
     3361 gtcttagata tatttcttga acttaaaata caaatacaaa tagtgtatat ataattgcgg
     3421 atattatgtc tgttctatca aatttaatac ccgtcaatca gaaatagaat tcgtacctta
     3481 atctcttctt aaattgacag cggagtattt atttgatatt tattataagt ttattataca
     3541 ataaatttac atacctccat tcataggcat attcattgaa ctagaattca tattcatatt
     3601 acccgtaata gtatttttcg aaggaaaaag agtaaatgtc atttcagttg ttttactttg
     3661 attattttga atattactat tattctctac tattttttga gcaatatcca taacggtagg
     3721 ttttgttaac ataatatgaa ctacatcttt ggaggaattt gttctatcta ccaataagga
     3781 tttatttgtt aaatcaatat tattgacagt acctttatat ccatttgcag ctagaggact
     3841 cggttttgga atagatattt cctcatggac ataaagattc acttttttca atctatcaag
     3901 attccaatca aaaggcattg caaattttaa ttcattagcc gacgaatcat agttaaaatt
     3961 atttaatgca tcataataag aaattgcatc tatagtataa gctgttccat taatttcaag
     4021 atctttatcg attgaataac ctacactaag ccaaccttca tacactggtt ggttatcggt
     4081 tggaataaag gttctatcaa aatcaagtgt ttctatagta acaataaagt gataaagacc
     4141 tccttcggca aatatgggtc ctgttgcaga gacaggatgt tccatatcgc cagtataagc
     4201 tagtaaaata gtatcttgtt caccatttat tgtaatgtga tcagcactag atggtctcat
     4261 ctgtattcct agattgccgg tatgagaatg aaaagtatct aatagtagac gctttccact
     4321 tttttcaatc tctataagat atgttacatg ttttagagtt tcattggtag cagtatcaga
     4381 taatcgaaat tgaattaaag gagtttgacc accttttaag gtgtctgttg taactacagg
     4441 aggggacatc tttatcaaaa gtgaaaggtc tcttccagcc atggatgctg ataattcttc
     4501 ctgaaacaaa ccatctccaa agatgggaat aaatataaaa gtattgacaa atagcagagg
     4561 aaaaatggaa aatataatag aagtaaatct aatagatggc ataataacat taataatgat
     4621 cacttataat tttataaatt tatggttttt tagtattctc taaaagtcct tgtcagtttg
     4681 aaagctgtca aaaaccgtag ggtttgtatt ttacaaagtt catattacat ctaaagtatc
     4741 ttaatgcatt aggtttctct tcaacaaaca gtccacaaaa tggctttttt tccatttttt
     4801 aaattttggg catcaatctc tttgtaatgt ataaatgaaa aagtaatact gagaggtaag
     4861 aaaattaaaa taaaatttga atattttgat ttcgagttat ttaattatgg taaaataatc
     4921 aataactttt aatatatcat tacctggggt aggtggtaaa catgattgcg gatctatcac
     4981 ctcttgttcc tttttctgcg tgatttttaa ttgacaattt aagttaatat cataaaattt
     5041 tacatctaat cttttgccat ctatttttat gtctgcaaaa ccaaatcctt tgttataatg
     5101 attagcaaca taatatggta cgctttctat tctatggagt tcgtctcctc ctactccaac
     5161 aaccaaaaag atagttcctt ggggattagt aaatatatta ttattactag tactactact
     5221 atttttgtct acgataggtt gagaaatatc taatttattt agggaaaggg gtagagttct
     5281 gctataaata tgattatgac cttggattac tagatcaact ccatatttat caaaaatagg
     5341 ttgatatttg tctctaataa tatactcttc aaattgttta gataaaggag aatacattgg
     5401 cttatgaaac atgacaaata tccaatcaat acttttattt tgactagttt tctctaaatc
     5461 ttcaagtaca aattgatatt gttcattgtc tacctttagg gctggaactt ttgcattaga
     5521 ttctataatc ttgtcaagtg ctggaatttc cacatcaatg gaattttgtt caagtaaatc
     5581 tgctatatct attaagggaa aacgttcaag tagtggttct ttctttttat cattcgcatc
     5641 tttgttatct ttcttacctt tattatcatc tattgtattt gtagtagtag tagtttcatt
     5701 tattattgct gtagattcta gggtatcaac agaaagctct aattgtgtat ctagtacaag
     5761 tacatggaca ttttgataat caaaagaata atatggttta tttaggttat agtgattcaa
     5821 gagtgatctt tccaaccttt ctccttttgc accttcttct tcataatcat catgattacc
     5881 tattgctatc ttagtttttg aatctagtga tttggtcata ttgaaccaac aatcaggtgt
     5941 ctttttataa ctaagatctc ctgcaacaag gaataattca ggatctaatt tctctatatt
     6001 tttaatattt tcttgagctc ttatagaaca gccaacatct gcagtagcta taatattaaa
     6061 ctcattctca tttttgataa tctgattttc gatcgcaaag gaattattat ttatacaaaa
     6121 gatagtcaaa acaataaaac taatagtaag aaagagcata aattgtgaca cattgagcga
     6181 aggtttgaaa aatgacatac tcatttcaat tcttagaata aaaacaataa gtactttcac
     6241 ttaaaaggaa tgtgatacaa atgtgtaact aaaccaaatg ttctataaaa agaaaaatta
     6301 agtttattac agacttgtac taacaaatga gacaaatatt ttatattttt gtttataaaa
     6361 atactattat tttgatttgt aaattaatat atatcgaaat cacgttctct atttatagaa
     6421 taaatgataa ataaaaatag gaccgttttt atattattat tatcttttcc acttttactt
     6481 tttacttttt gtaatattta tgcacaacaa aatcaacctt tggttgctga aaatataact
     6541 ctttttgatc ccaagttgaa agttgagtta gtgacatctg aacttgattt ccctactaca
     6601 atggcatttt tgggtccaga tgacttttta atcctggaaa aaaatactgg aaatgtaaaa
     6661 agatttgtaa atggaactgt gcttgaaaaa ccacttttac atgttaagcc aagtataaaa
     6721 gatgaacgag gcttgctagg aattgctgtt agcgagaaga aggaaatttt ttatgagaac
     6781 acttttttaa tcgaaaataa aaaactaaca cataacgtct ttctttacta tattgaatgt
     6841 caaaagaaaa atacagattg tgagaatcgg atttataaat atgatttaga taatcaaaaa
     6901 aatgtccttg taaatccaaa attattagta ggtgtacctt catttccaga tccttctcat
     6961 attggtggga tactagatat tggacctgat gaaaatctct attttactgt aggaaatttc
     7021 caaaatacta ttccatcaaa aatttacaaa accaaaactc agaatgtcga ggatggtgaa
     7081 ccagtagatg gtagagctgg aattttaaga ataacccaag gcggcaagcc agtgggtgct
     7141 gaaggaataa taggaaatga gtatcctctt aacttgtatt atgcgtatgg tgtaaaaaat
     7201 agttttggaa ttgattttga tcctgtgaca ggcaagttat gggatactga aaatggacct
     7261 cagtttggcg atgaaattaa tttggtagaa tctgggttca acagtggtgc tgataaactt
     7321 tatggcattt gggaatctaa tgaatttgga gatattttaa aggacaaaaa aggtgaatct
     7381 atagtagtag gaaacaatcc tgcaaatctg gttaattttg atggaaaggg acattatagt
     7441 tctccagaat tcatatgggt taaaacaata gctccaactg cattattatt tcttgattca
     7501 gacaaattag gatctaacta taaaaacgat atgtttgttg gatctgcaga tggtggtcgt
     7561 ttattttatt ttgatctaaa tgataagaga gatggattag ttctaaaagg taatttaaca
     7621 gataaaattg catacgataa gtcagagttt aatgatatac tgtttgctga agggtttagt
     7681 ataattactg atttaaagca aggacctgat ggttatctct atattatctc aggactaaag
     7741 caatcaaaaa cagcaaagtt tggcgcggtt ttccgaatag tcccatctgg aaagagcacc
     7801 accaatcctg acaaattaga tattgtaaaa caacagacac aagaggaaaa aagcaagatt
     7861 atatcgagta taactaatac caatactacc actaatactg atctactatc aaatcaagaa
     7921 gattcacctc ctaaaaaaac aaatacaaat gtacttgttg atatagctcc aaagccatct
     7981 aacacaacta aaacgataga cgtaaaagca tgggataatc ttataagaca gaacttgaac
     8041 aaacctcaca ttcaatccaa ataaaggata ataaatcaga atacgaaagt aatgcagaaa
     8101 gaataataaa tgttattgtg gtataattat agcaaaatac aacaattaaa ctctgaagtt
     8161 attgtagttt atatatagaa attataaatt atttttttga acaaataatt tatcagttat
     8221 ataaaagacc ttacataaat taataattaa taaatgtcta ttacaaactt ttcataatat
     8281 ctctatcata ttattctatt ttttgaaaat agttaagaag gacaaatata ctaacactat
     8341 taaaataaat atagttaatt attattgtta ataatagtat ctttctatga atttttcatt
     8401 gcattgtaat ttataaaata aaaaatgaat atttttctga atattttatt atttccctta
     8461 taaagatatt taaaatactt tttatagtca tttaaattaa taatttcaaa tgaatttagc
     8521 tattgataca aagcgtttgt ttgtcatatt ttcctctatt tctttatctt tattgttagc
     8581 agcagtatta acaacaggta cagtcaatgc atttgaaaat gctatagaat acaaatttgt
     8641 aaaaaaatgg ggaggagcag gatcggcgga tggtcaattt ctacgacctc atgatttaga
     8701 ttttaataaa gaagaaactg tactttacat tgtagataga gatggaagtc gtattcaggc
     8761 atttgataaa gatggtaaat tccttttcaa atttggtaaa gatggaaccg gtgatggaca
     8821 aatgcatgtt ccatatggaa ttgatgttga taaaaaaggg aatatttggt tagcagaccg
     8881 tgctaatgac cgagcacaag agtttgatag taaaggtaaa tttgttttga aatttggtaa
     8941 tgtagatgga gttccaagtg ctgaagaagg aaaatttgat aatccaagac atgttgcagt
     9001 aagtaaagat cttagatatg tttacgtagc agattctaaa aataatcgta tttcgcaatt
     9061 tgatacaaag ggtaactata tcaagtctat tggacaatta ggaacaaagc caggagactt
     9121 taatcttccc actacaatag aaatagattc taaaggcaat ttctttgtaa atgaaagagg
     9181 aaacgagcgt atccagaaat ttgacagtaa ttggaaacca attttaatgt ggggttctaa
     9241 gggaagtggt gataaccaat tttgtcatat tgaacacatt gctctagata aatacgacaa
     9301 tgtttacgta actgatccac aatccgatcc aggctgtagt caacaacctc gagtattaaa
     9361 atttgacagt gaaggcaaat ttattacaaa atggggatct tttggtaaag gagacggtca
     9421 atttcaggat cctgaacact tagcagtaga ttcagaaggc aatgtgtata tatctgatag
     9481 aaaaagagac gatattcaga aatttacccc aatcagtgaa acaagtaacc aatcagtgaa
     9541 tatgtctaat actaaactct caacaaaggt agaaactcct aaaaaaacaa atacaaatgt
     9601 acttgttgac atagctccaa agccatctaa cacaactaaa acgatagacg taaaagcatg
     9661 ggataatctc atgcaacaga aattaaagta ggcagtaatt cagtagattt ggggattggg
     9721 caatttataa atattcacct ttactatgcc gaatttataa atatagtatt atagtattcg
     9781 ttctataaag ttaaataaaa tgaataaaaa gagaattact atatttgctt tatcttttcc
     9841 acttttactt tttacttttt gtaatattta tgcacaacaa aatcaaccta atggtgatga
     9901 aagtatagtt ctttctgatc ctaagttgaa aattgagtta gtagcatccg gacttgattt
     9961 ccctacgaca atggcatttt tgggtccaga tgacttttta attctggaaa aagcaggaac
    10021 agtaaaacga gttaccaatg gcgtagtact agataaacca attttacagg ttgacgtaag
    10081 tgtaaaagat gaacgaggct tgctaggaat tgctataagt gaaaagaaaa atttcggtaa
    10141 tgataacggt acatctatta aaaataataa tatatctcat aacgtctttc tgtattatgt
    10201 agtatgtaag gagaaaaaca caggttgtga gaatacaata tacagatatg atttggacaa
    10261 taaaaataat gtcttgataa atccaaaatt attgttaagt attccatctt ttcctgatcc
    10321 tgcacatatt ggaggaatta tagatattgg accagataat aatatttatg ttaccgtagg
    10381 aaatttccaa cacactattc catcagaggt atacagaacg aagactcaaa attatgagga
    10441 tggtgaggct gtggacggaa gggctggtgt tctaagaata acccaagatg gcaaacccgc
    10501 tggtgaagga atacttggaa ctgaatatcc ccttaacttg tattatgcct atggagtaaa
    10561 aaatagtttt ggaattgatt tcgatcctgt tacagaaaaa ttatggttaa cagaaaacgg
    10621 accaaaattt ggtgatgaaa ttaatttggc agaaccagga tttaatagtg gttctgataa
    10681 gatattcggt atttggagag ttaatgaaga aggtaaaaaa ttgcaaacag atggcaagga
    10741 aggtgatggt gaatatgtaa ctgtaggtaa taatcctaaa gacttagtat attttgaagg
    10801 aaagggacat tattcccctc ctgagttcat atgggataaa tcaacacctc caactgcgct
    10861 attatttctt gattccaaaa actttggcac tgaatatgaa aatgacatgt ttgtgggatc
    10921 tgtagatgga caaatatttc attttgattt aaatgacaat cgaactggac tgcttttaaa
    10981 aggtgtttta aaagataaaa tagctgcaga tgatacagag tttgctgaca tactattcgc
    11041 aaaaggtttt agtattatta ctgacttgaa gcaaggtcca gacgggaacc tatatgtagt
    11101 atcaggacta aagcaatcaa aatcagaaaa attgggtgct gtttatagaa taaaataatt
    11161 attccttaca ggataattca taaaaataaa cgatattttt aaatcaaaat aaacttcaaa
    11221 tctaacatta caagatataa taataatttt tactcatacg tctctggtct aaaaaaagta
    11281 aagatttacc tttatctttg ctaatgtaaa ttcactttat agtaaatgtt tttaatccga
    11341 caaatataga agtcccttaa gggatatatt tatagaatta agttggaaac aaatatgaaa
    11401 cctaataaag ggaacattac ttatttatgt ttagtaattg ttattatctt tgtaaccact
    11461 actttgcttg tatcttctaa tgacttatct tttgcaataa aagagaatga aaacaaagat
    11521 gatagtcaag ataataagaa agatgttcct atctcctcaa caaacacaac aaaggaaatt
    11581 cttaatctta aattaaaatc ggatgttaga aacgatatag aagatgcaca ttatccaaat
    11641 caattttata cgtgtgggta tccacaacaa ttaataacgg attacaattc ttttgcaaaa
    11701 tttgattgtc aatagagaga gttactaaac atcgatcatt atcgcattat tttgattgaa
    11761 ttgttaaata tatctctatc agttgggttt tcttctgaat cgatctggaa agattcagta
    11821 attttgtaga gtatttttgt aattattcat ttctttcttt gatctttgat ctttttataa
    11881 acttgtaaat aatgatcttg acatgaatac cttattttga tttgtttggt ttcgtctagt
    11941 attccagcgg ttgctttttg tctacaaatt tcacaaatgt ttttcgatat ttcaaaattc
    12001 attttatttt attttattac tttattttta ttctctttta ttttattctt tcaatattaa
    12061 atcattgtca tgcatatatt tgaattatta ttaaaaatgt aactacaata agaaaatata
    12121 cataaaagtt accgactgtt tatagtagta gacattttct tgacaagatc tacaaatttc
    12181 tttctattag gactggaatt agtaaataca atttctgtaa acccaatatc tatatatttt
    12241 tgtaattttt tgattccttc ttcttcatta gttattataa ataacttttt ttctaacgaa
    12301 tcatctccga taacttgacc attttcttca atcttccttg gatcatgtac atcaacgtca
    12361 aagaatgctt taatcataga acctctccaa aatcttattg aatctattgc tttttgttta
    12421 tcttcatcat aagaagctgg aataaataat attttttcta attggtcata attctttcct
    12481 gctttagaag ttccctttct aactgccggt aaaagcttat cttttatttt atttatatct
    12541 agctcattag ttacaaaacc atttccttcc tcaccagcta attgtgcaga ttgttctcct
    12601 aaaccagcta tatatagagg aattaagtaa tcaggtttag tatataaatt agaatcttta
    12661 actgaataaa attctccctt aaaattaacc caatcttcag tccataattt ctttatcaat
    12721 tttatagctt ctcttaaacg tttgaatttc tctttatttg atggccaaat atttccggtt
    12781 ggtacttcat ttagggcttc tcctcgtcct actcccaaaa atactctatt tggaaacata
    12841 taagataatg ttgcaaagac ttgtgccact attgcaggat tatatcttag tatgggtgct
    12901 gttactcctg taccaataac caattttgta gtctttgcta atgcagcacc catccatggc
    12961 catgctgctc caccagaagc tccagtgttc caccagggca tataatggtc actactccag
    13021 caacgttcta tctccttttt atcgagatat attgcatcat ctaacaattc attaggatta
    13081 aactgttcat gtgctgcttg aatgcttaat cttattttta cccttgttga gtttgacatc
    13141 taatattcta ttatatatta agtaaataag atttcaaagt tttaaattct tttacagata
    13201 aaatgacaat caaaatttta ttattatcag ataattaatg ctatttatag aagaatcaag
    13261 ggttgaaagt ttgggtccta tttttaggtc aaatttgttt catatcccac tggtcccatt
    13321 ctataagtgg gttcatatac tgtgagtttg atattagatt ttgtaaaatc aaggagcaac
    13381 gaaggataaa atgccactaa aactatctac aacaatagga aaaatccaaa atattcctaa
    13441 tataaagaat aaagagatag taaatgaatt tggggaatat at
//
LOCUS       DASVEV010000249         7660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1094665, whole genome shotgun sequence.
ACCESSION   DASVEV010000249 DASVEV010000000
VERSION     DASVEV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094665"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF222_10810"
     CDS             complement(<1..249)
                     /locus_tag="VF222_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7142956.1"
                     /translation="MSYKIKEESSPRHKRTRSLENVLIMQGGGSLGSFACGVFKALVK
                     QKIRIDIVAGTSIGAVNAAIIAGSKSDQPEKDLEDFWLE"
     gene            455..640
                     /locus_tag="VF222_10815"
     CDS             455..640
                     /locus_tag="VF222_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142957.1"
                     /translation="MKIIEQTHEESNAYSLFVYAIRSQVTRDYYLRRLRILFSEPTKN
                     IEIFIGFNSQSITVAIY"
     gene            complement(1036..1197)
                     /locus_tag="VF222_10820"
     CDS             complement(1036..1197)
                     /locus_tag="VF222_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142958.1"
                     /translation="MESETYITTENTKNSTYTEVPCDICGLLFDSPDIKEEHKKLVHV
                     EHKRPSGVG"
     gene            complement(1354..1566)
                     /locus_tag="VF222_10825"
     CDS             complement(1354..1566)
                     /locus_tag="VF222_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142959.1"
                     /translation="MDIFDSIGNGFISALAREVFSRLFNYTIQNKEKIIFVFKKIFKK
                     TRDYDLSNNHPNTPFTYNGKEFVTHT"
     gene            complement(1808..1972)
                     /locus_tag="VF222_10830"
     CDS             complement(1808..1972)
                     /locus_tag="VF222_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142960.1"
                     /translation="MQHDDIIEIAAATTRRIQLSIRRIYEYEENIEVLIATKTNSKPE
                     RQENFTISIE"
     gene            complement(2099..2443)
                     /locus_tag="VF222_10835"
     CDS             complement(2099..2443)
                     /locus_tag="VF222_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142961.1"
                     /translation="MAVYDSAGFSSQQALQGVKIYHNLKIDIHDSTFDYSRISAIVNE
                     AKSTHIGKTRWKQTFTPDEKFLNICKSIGYSPNTQYQLLQLIIPVEEKIMEEVKRKLS
                     NFLFASASRANY"
     gene            2583..2864
                     /locus_tag="VF222_10840"
     CDS             2583..2864
                     /locus_tag="VF222_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142962.1"
                     /translation="MKTIVIVFLLLIGILFVIPLQQVFAQIGASEQIMSQMRFTHSQL
                     TNNIDQVKQLVKSNNTSEALTLLEGMDVKIGHMNTMFNDLIWELSNKGH"
     gene            complement(2941..3420)
                     /locus_tag="VF222_10845"
     CDS             complement(2941..3420)
                     /locus_tag="VF222_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142963.1"
                     /translation="MASTNNQNINPRPCVNNCGIQIYWNVATNEYWEVFTKKKHICPN
                     RSNKPQVTNTTATTTAAKPNYYNGRKSFTNVKPKMSNSFELLTGPIDIIQKKYEMLSD
                     IVIVEYGGKVHGSQRDRDPKTGLIDLLVYYEVPLGQRDEVKKKFENFTKSHNIVLQK"
     gene            complement(3436..3615)
                     /locus_tag="VF222_10850"
     CDS             complement(3436..3615)
                     /locus_tag="VF222_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142964.1"
                     /translation="MSSNNTKECKHDVLSETDLSCTVCGMSRYDQLQNKKRLTKKEEK
                     ELEDYVNRKHWGLID"
     gene            complement(3635..4081)
                     /locus_tag="VF222_10855"
     CDS             complement(3635..4081)
                     /locus_tag="VF222_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142965.1"
                     /translation="MTDRDYYLDMAINAAIEYVKKKEGIELEGKDRLRYKSIIIDDEE
                     FHVFAFFKYGLIIDYDNNTDELVVNELDGVKSGKYTKGDLAFLKSSIDRFGSLEEFQK
                     SYKEFKQEHSADTIKDIQEINKEFRKEFGIPDRMAIPGDLDNLIDQ"
     gene            complement(4251..5540)
                     /locus_tag="VF222_10860"
     CDS             complement(4251..5540)
                     /locus_tag="VF222_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142966.1"
                     /translation="MTTFLIIILTISTIVGNSFAHTFTNNESAAFLALIDNMKIQASL
                     IQQSISSGNIDIAKQHIDKLKELYSNHTNEEIAEKNERIANEISTTINYFTPSSNQNN
                     FSQSQIDNNVQNLNAILDESISVRIPQDVLDNSTVHALHFVELVNAVDMNYNNTLVEQ
                     DIQNMTLVNNNKSRSQIEDIVSYNTTRDLLNVAIDLYEAKLKSKIIVNNNNSGTFDKL
                     QEGIKQLKTSIELKKLSSEITNIINGAVYPALQELYNFKLGEEEDVHTEEKEHSEAHK
                     TVRDSVTVLLQDLSIPATDFIHLYDSTPYNIMNGHVALKVPCGDDSTSPITVLIGSAP
                     NMTAGTLENLPTLSTPGEQCLYHVDLIPDGNVTTITDIAISNTGEEDIQFPPTATVVI
                     GINKVTKGEHGGEHSESTEEHASTTNTAAAAAEAEHT"
     gene            complement(6010..6882)
                     /locus_tag="VF222_10865"
     CDS             complement(6010..6882)
                     /locus_tag="VF222_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX7142967.1"
                     /translation="MDSSKNNDENNKLIDHLNELLSIENAVTERLERRILETPKQESK
                     NLLQQHLKAEKEQQRRLMDLISSYGKKPTNSKADIISLHSLINEARDKMKKDNVTKAK
                     DTNIITEILSKTINDNKTNVNNTIMTSEESEILNTKEDALIKNNEISSYKKILKIAEG
                     VMDKDAISIIKQNLQEKESIYDKLKSFESKMQNEIGENNNDNHNESFKVGSAVADMLT
                     SYWNSQENPSKVYLLNRRVHHGAIGALLGLSSLYKKNPLVTGILSGLGAGLKKDDSKD
                     SKEWFLFRKKEDEK"
     gene            7473..>7660
                     /locus_tag="VF222_10870"
     CDS             7473..>7660
                     /locus_tag="VF222_10870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7142968.1"
                     /translation="MKYDWDAITYDRISDAQESWGQEIIEYRKWKGNEIVLDAGCGSG
                     RTTRILSIKVPQGKVIAV"
BASE COUNT         2415 a         1168 c         1009 g         3068 t
ORIGIN      
        1 ttcaagccaa aaatcctcga gatctttttc tggttgatcg ctcttactac ctgcaatgat
       61 ggcagcattt acagcaccta tagaagtacc agcaactata tctattctta ttttttgttt
      121 tactagtgcc ttaaaaacac cgcaggcaaa agatcctaag gatccaccac cttgcataat
      181 aagaacattc tctaaagatc ttgttctttt atgtcttggt gagctttctt cttttatttt
      241 ataactcata gatattataa tatctttttt caaaggctaa aaattttctc atttgtattg
      301 ttaattatat tgtttttact accatcgtaa tatagaaatg tttggggaca aaaatcaggt
      361 caaattagtt tcacatccca ctggccccat tgtgatatta gttctctaat tggattcact
      421 cgaaaataaa aaaataaggt gaatcaaaaa ataattgaaa ataatagaac aaactcacga
      481 ggaatctaat gcatattctt tatttgtata tgctattaga tcacaagtta caagagatta
      541 ctatcttaga agattgagaa ttttattttc ggaaccaaca aagaatatag aaatttttat
      601 aggttttaat agtcaatcta ttactgtagc aatatattag atatttactt ggaatgtcct
      661 tttatctttg ctaaatatat cttcttcagc ctttgtttat acactaaagc acgattatag
      721 aatatcgtta gataattaat atgtgattcc ataaatttat cataattttc taattcttta
      781 cattctaatg catgtgaacc aatattgtta tcataatcat catcattcca ccactcgtag
      841 agaatgacta aaatgttatt tcacataata agattgttac agagtacaat acttaaaatg
      901 tactttggtc atacaacttg tatatctcta gtattttatg ttgattaatt aggactatat
      961 actagaaaaa ggtttcaaga aaataagaag aaataaaatt taattctgct attattataa
     1021 aatattttta atagattaac ctacaccact gggcctttta tgttcgacat gtactaattt
     1081 tttgtgttct tccttgatat ctggagaatc aaacaaaagc ccacatatat cacatggaac
     1141 ttcagtatat gtgctatttt tcgtattttc agtggtaata tatgtttctg attccactat
     1201 tacaataaca gatttgttat taataataat ttagttaaaa ataacactaa ttaattatcc
     1261 taaataaaat aagaataatg ttctagaaga aaagattgat tgttgccatt tcagataagg
     1321 tccactctct agattgttaa ctgtactgat tgtttatgtg tgagtaacaa attcctttcc
     1381 attgtaagta aatggagtgt tgggatgatt attactcaag tcataatctc ttgttttctt
     1441 aaatattttc ttaaatacaa aaattatttt ttctttattt tgaatagtat aattaaatag
     1501 ccgtgaaaag acttctcttg ctaaagcact tataaatcca ttaccaatgg aatcaaagat
     1561 atccatataa aaaggctaaa aaagaccttt aaaagattta tgcgatcttt aaatacaata
     1621 tgaacattat tgtattattt caacttcttt ccttgataac ttttaattct gtatttctaa
     1681 caaaattatt aaattttcgc ttaacttcat ctctctgccc taaaggtact tcataatata
     1741 caatcaagtc aataagaccg gttttagggt ctctatccct ctgtcttctc taactcatag
     1801 accagttcta ctctatggaa attgtgaaat tctcttgtct ttctggtttt gaatttgttt
     1861 ttgttgcaat caaaacttca atgttttcct catattcata gattcttcta atagaaagtt
     1921 gaattcttct tgttgttgct gctgcaatct ctattatatc atcatgctgc attttattat
     1981 tctcttcttt tataataaaa tttttgtaat ttaaatcagt tagacaaaaa agtttgttta
     2041 tttgttaatt tgaatcctac tttctttcta tatctgtatt atattgtata ttattatttt
     2101 agtagtttgc tctactagca ctcgcaaaaa gaaagtttga taactttctt tttacttctt
     2161 ccattatttt ttcttcgact ggtattatca attgcaataa ttgatattga gtattgggac
     2221 tatagcctat tgatttacag atattgagga atttttcatc tggtgtaaat gtttgtttcc
     2281 atctagtttt acctatatgt gtagattttg cttcatttac tatagccgat attcggctat
     2341 agtcgaatgt tgagtcatgg atgtcaattt tgagattatg atatatttta actccttgaa
     2401 gtgcttgttg tgatgaaaaa ccagcactat cataaactgc caaaatacca tatgcattct
     2461 ctaaatctct gagttctcgt ctatctaact tttctattag tgttattctt ttgacatcat
     2521 tatcactata ataataatga aatattctaa atctttaaaa gattaaattg tataataata
     2581 ctatgaagac aattgtgatt gtatttctgt tactgattgg aattcttttt gttattccac
     2641 tacagcaggt atttgcccaa ataggtgctt cagaacaaat aatgagtcaa atgagattta
     2701 ctcattccca actaacaaat aatatcgatc aggttaaaca attagtcaaa tccaataata
     2761 cttcagaagc ccttactctc cttgaaggta tggatgttaa aattggccat atgaatacaa
     2821 tgtttaatga tctcatatgg gaattgtcca ataaaggcca ttaactaatc cttcttcctt
     2881 cattacatag aggaataact cacacaaaca aattttttct tttttaatat aaaatgatta
     2941 ttatttttgt agaactatat tatgactttt agtaaagttt tcaaattttt tctttacttc
     3001 atccctctgt cctaggggaa cttcataata taccagcaag tcaataagac ctgttttggg
     3061 atctctatcc ctttgagagc catggacctt accaccatac tcaacaatta caatatcaga
     3121 taacatctca tatttctttt gaattatatc aattggacca gttaacaatt caaatgaatt
     3181 agacatcttt ggttttacgt tagtaaatga tttcctaccg ttgtaataat tgggctttgc
     3241 tgctgttgta gtagcagtag tattagttac ttgtggtttg ttggatctat ttggacaaat
     3301 atgttttttc tttgtaaaaa cttcccaata ctcatttgtt gcaacattcc agtaaatttg
     3361 aataccacag ttattgacgc aaggccttgg attaatattt tggttattag tagaagccat
     3421 tttaattaat aataattagt ctatcaatcc ccaatgtttt cgatttacat aatcttccaa
     3481 ttctttttct tctttttttg ttaatctttt tttattttgc aattgatcat atcttgacat
     3541 tccacaaaca gtgcaagaca aatctgtttc agagaggaca tcatgtttac attctttggt
     3601 attattagaa ctcattcttg ttaatattta tgagttattg atcaattaaa ttatccaagt
     3661 cgccgggaat agccattcta tcaggaatac caaattcttt tctaaattct ttgtttattt
     3721 cttgaatatc ttttatagta tctgcgctat gctcttgttt gaattctttg tatgattttt
     3781 ggaattcttc cagactgccg aatctatcta tgctagattt aaggaaagca agatctcctt
     3841 tagtatattt accgctctta acaccgtcca actcattaac tactagttcg tcagtattat
     3901 tgtcataatc aataataagt ccatatttga aaaatgcaaa aacatgaaac tcttcatcat
     3961 caattataat agacttgtat ctgagtctat cttttccttc caattcgatt ccctctttct
     4021 tcttaacata ttctatggca gcatttattg ccatatctaa ataataatct ctgtcagtca
     4081 tttttctctt taagtacccc aggaaagtat catcttataa ataaattgat tttattttaa
     4141 tataggaact catgttaaat atttttagat tgcctcttca atagttattt tcagaggttt
     4201 acctttgaag tcttttaata tgtctttatg gtattcttga gggacgtaga ttatgtgtgt
     4261 tcagcttctg ctgctgctgc tgctgtatta gtagtagaag catgttcttc tgtactttca
     4321 gaatgttctc ctccatgctc tcctttcgtt actttattta tacctattac tactgttgca
     4381 gttggtggga attgtatatc ttcttctcca gtattagata tagcaatatc tgtaattgtt
     4441 gttacatttc catctggaat taaatcaaca tgatatagac attgctctcc tggagttgat
     4501 aaagtgggaa gattttcaag ggttcctgct gtcatgttgg gagctgaacc aattaaaact
     4561 gtaatgggag aagtgctatc atctccacat ggaactttta aagcaacatg tccattcatt
     4621 atattatatg gtgtggaatc ataaagatgt ataaaatctg ttgctggaat actcaagtct
     4681 tgtaataata ctgtaactga atctcttact gttttatgtg cttcactatg ttctttttct
     4741 tctgtatgga catcctcttc ttctcctagt ttaaagttat ataactcctg caatgctgga
     4801 tatacagctc cattgataat gttagttatt tcactagaga gttttttcaa ttctatagat
     4861 gttttaagtt gctttattcc ttcctgtaat ttgtcaaaag ttccagaatt gttattattg
     4921 actattattt tactctttaa ttttgcttca tacaaatcta ttgcaacatt caatagatct
     4981 cttgtagtat tataagagac gatgtcttcg atttgagatc tagatttatt attgttaact
     5041 aaggtcatat tttgaatatc ttgttctact agagtattat tataattcat atctactgca
     5101 ttgactaatt caacaaaatg cagagcgtgt actgtggagt tatctaaaac atcttgagga
     5161 atccttacag atatagattc atctaaaatt gcattaagat tctgtacatt attatcaatt
     5221 tgagattgag aaaaattatt ttgatttgat gatggcgtaa aataatttat tgttgtagat
     5281 atttcattgg caattctctc atttttctct gctatttctt catttgtatg attactataa
     5341 agttctttta gtttatcaat atgctgtttt gctatgtcta tatttcctga cgagatagat
     5401 tgttgaatta aacttgcttg aattttcata ttatcaatta aagccaaaaa ggcagcactt
     5461 tcattgtttg taaaagtatg tgcaaatgaa ttacctacta tagttgagat tgtgagtata
     5521 attattaaaa atgtagtcat taataattta agtaaataca tcttcttatg aggtacaaac
     5581 ctttatttca gaattatggt acatctctgg aattaatagc aattttattt acacaatatt
     5641 actactttat ttttatttat ttatataaaa agaggattta tttgttaaat tataacgccc
     5701 gaaaaatctt gtgattatga atttactctc taggtatatc gaagacaatg atagaaaaac
     5761 aagccaatac aatataagaa gaatttctaa aatctcaaat aatttctctt attattataa
     5821 agtatttgat cctatcgata tatgattcaa acttttaaaa attgggacca aaatttatgt
     5881 caaatttgtt tcacatccca ctatgaaaga tacaaaaatg gagctaatcc attgtatatt
     5941 ggtaggtcat gtttaactgt tttaatttgt taatttaatc aacatattta atatttttat
     6001 tttcaatctc tacttctcat cttctttttt tctaaataag aaccattctt tggaatcctt
     6061 ggaatcatct ttttttagac ctgctcccaa acctgacaga atcccagtaa caagtggatt
     6121 ttttttatat agagaagaca aaccaagtaa agcaccgatt gctccatgat gaactctcct
     6181 attgagcaaa tatactttag atggattttc ttgagaattc caataagatg tcaacatatc
     6241 agcaactgca gatcctacct taaatgattc attatgatta tcattattat tttcccctat
     6301 ctcattttgc attttagatt caaaactttt tagtttatca tatattgatt ctttttcttg
     6361 taaattttgc tttattatgc ttattgcatc tttatccatt actccttccg ctatttttaa
     6421 tattttttta taagaggata tttcgttatt ttttatcagt gcatcttctt ttgtatttag
     6481 tatctcagat tcttcagatg tcattatagt gttattgaca ttagtcttat tgtcattgat
     6541 tgttttggat aatatttctg ttattatgtt tgtatcttta gccttagtaa cattatcttt
     6601 tttcatttta tctcttgctt cgttaattaa tgaatgcaaa gaaataatat ctgcttttga
     6661 attagttggc tttttaccat aagaagagat tagatccatc aatcttcttt gttgttcttt
     6721 ttccgccttg aggtgttgtt gtaacagatt tttcgactct tgcttaggag tttccagtat
     6781 tcgtctttct aatcgttctg ttacagcatt ttcaattgat aaaagttcat ttaaatgatc
     6841 tataagttta ttattttcgt cattattctt agacgaatcc atataacatt atggaaagca
     6901 ttaaacttaa agcttttttg atacaagtaa acaaatatct aaaacatcat ctcttaatat
     6961 ctaatattat acatacaaaa aatgattttt ttaatccatt aaaaatgatc ttcaaatctc
     7021 ttcttaggtc aatttgtttc acatcacact gagatagata taaagttgta gctaattctt
     7081 gtatctttta ttttcttatt taactagtat tatagaatat tttatttgaa aatattgaac
     7141 tcttttcctc gagtataaaa tcctctaatt atcttttcat agcttattct ctgttctgat
     7201 ccattatttt ctatattctc attggctaaa tttggattaa tttgtatttg aatcaaatat
     7261 attgttgttt atattttgtc catatacaat aacaatatta taaaggataa agtctgacaa
     7321 acattgagac aattaccaac atacatataa tttttgaata ttcggatttc ttttatatat
     7381 tttttaataa tcattagatt atattgaaaa attattcatt aacaacatct tacaaaataa
     7441 ctttatataa ataataagag cgatatctat taatgaaata tgactgggat gctataacgt
     7501 atgatcgtat ctcagatgca caagaatcat ggggacagga gataatagaa tatagaaaat
     7561 ggaaaggaaa tgaaatagtt cttgatgctg gttgtggttc tggaagaacg accaggatat
     7621 tatcaattaa agtcccacaa ggaaaagtta ttgcagtaga
//
LOCUS       DASVEV010000250        18649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1095393, whole genome shotgun sequence.
ACCESSION   DASVEV010000250 DASVEV010000000
VERSION     DASVEV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 18649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18649
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095393"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1206)
                     /locus_tag="VF222_10875"
     CDS             complement(31..1206)
                     /locus_tag="VF222_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7142969.1"
                     /translation="MNNHNNNNNSISRRKPIENVLVLQGGGSLGAFACGVYKILHKKN
                     IKFDIVGGTSIGGINGAIIAGSKNDNPVKDLEDFWMELAESSYTVIPDIVTLDYDDKS
                     KGIGLKTLPSASLNAAIYGVPKMFVPRWFQTNSITKESLFGEQQLPWNWTYLYDNSPL
                     GKTLEKYIDFKKLSPEITSSSCSTKTMRLIVTAVNVLTAEPLTFDSFKIQIQLKHLLA
                     SIGYPNYGFSWIEVEDGVYGWDGALLSNTPMREVLLQSPSYDKNIFIVENYSRKINHL
                     PSDMTEVQSRIKDILYCDKSESLSQISKLITRQLGLIENMYSELNFSKIDSKKLEYIK
                     KEYQQLVNNYGAKILNITRISRGTTESPYPLQNADFSIHTVKALIKEGESKAMEILK"
     gene            complement(2425..2691)
                     /locus_tag="VF222_10880"
     CDS             complement(2425..2691)
                     /locus_tag="VF222_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7142970.1"
                     /translation="MGNRSRTEIVGSILDAANGGASKTKIMYTAFLSYNQLNEYLTSL
                     IENNLIEYLDGTHKFKTTEKGLFFLKIHNEIGELLQQTTVENNN"
     gene            3522..4100
                     /locus_tag="VF222_10885"
     CDS             3522..4100
                     /locus_tag="VF222_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7142971.1"
                     /translation="MQVSKIPNPNCLILSLFCVIGLLFLLVLETDFAGNKYNIMKSVW
                     ADEINGTENADSITGTLSQDTIKGLQGNDTIGGKEAGDDISGGDGDDALYGNEGRDWL
                     RGGSGNDHLEAGEGNDMLFGDRGDDTLYGGPGNDKLTGGPGKNMFICGPGPDMVIDFN
                     VTQQDSAPENDCENLKNNTTNVNSTSSLNNNT"
     gene            4769..6163
                     /locus_tag="VF222_10890"
     CDS             4769..6163
                     /locus_tag="VF222_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7142972.1"
                     /translation="MLLEFKWYDLIKFSFIFSIVFFLICLQTLTFQVVFSTNNNKSFE
                     FTNESNLTTNTFKKNFNNISDGDDNNTSPLNQNQTLNEQKIKIVAAGDFGCRPVAQNN
                     IKQIELEKPDIFLVLGDLSYEPTMDCWYDMTKGLDSRIKIAIGNHEDEEEKAKGGSKE
                     LKDSLLKHYNLQNSYYSFDYRNIHFLVLDTQLEFSFDVFKMLEEEEEKARIEETSNNK
                     DDESNKPEAKYYATTLKDLLTKYNIKGEIPPYHLINDEVIVEDVPLNTEQYKFAVKDL
                     EKANNNSSIDWIFVMFHKPFYSSLTSHMQEYIMREKYQPVFDKYGVDLVLQGHNHLYD
                     RTLPLRFNPQNISKPIVDESNNTNKFVNPEGSIFSVIGLGGRSSHIFLNQPDYVVKQS
                     NGFGFLSIEINGKELDAKYYDIGYKCNEQKLKEKDLEEEGDFIIYDMSSCKNDNKSKD
                     SLKIIDHYSISKVS"
     gene            complement(6766..7215)
                     /locus_tag="VF222_10895"
     CDS             complement(6766..7215)
                     /locus_tag="VF222_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142973.1"
                     /translation="MIIRLEDSFIQLANKLNKPFLEGDKDKLAKAIELNYNKIFSIIS
                     NLEKSKESKPTVIELGNINGRYFALNLYYYQDEMDDDIPLPIKQLLPTISPVRSDLMA
                     TVSSEIPDDEGQNYIPVGDIYDDGDIHTHYVDEVIQEDSGLRSIDEI"
     gene            7432..7683
                     /locus_tag="VF222_10900"
     CDS             7432..7683
                     /locus_tag="VF222_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7142974.1"
                     /translation="MKRFFKTFTSKEICYTTKDTEVIVALSIESREKVDDMINKVIEA
                     GGKEDREPQDQGWMYSRSFEDIDGHLWEIIYMDESTVTK"
     gene            8121..8348
                     /locus_tag="VF222_10905"
     CDS             8121..8348
                     /locus_tag="VF222_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142975.1"
                     /translation="MVSTKPSNRKIINISYYYILMMPRDLKHVIEGLEGIKNKATEVV
                     LRRKEEDNMVDVGTGKVTIPNIKHFYLLNDL"
     gene            8338..9318
                     /locus_tag="VF222_10910"
     CDS             8338..9318
                     /locus_tag="VF222_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX7142976.1"
                     /translation="MIYKKLIISEDVINDIVEESNRHQNVETGGLLYGKLIGEYIIIL
                     KLIHFSSNARRTQVYFEMDEDFAINITKQMEKSNLAYLGNWHKHLGYGGPSNGDDKQA
                     ELFLIQNSHKRNYLSLIIDFYNNDYNLIATNYYFDKAQFLKKDIQIQRIINKQELNKM
                     FNAKTVNIRNFIDNLSKNLEEHTGKTFIHSQSNKSQNYKLFYEEIYNPQDNGSISTNK
                     IVYSVFIKFPYLNISMTDKISIGVTLDHKFTRIFEEITFSDIFFNIVELLPKIKNLFD
                     KSQYYIDISSYSYNKKENGDLKSRTKTFFRALKSLSIFTFHLKEKVKNVN"
     gene            9377..10558
                     /locus_tag="VF222_10915"
     CDS             9377..10558
                     /locus_tag="VF222_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX7142977.1"
                     /translation="MIFPEVKQTLLDNKKFKSNEKFLFSVNGSIMDDSTYQIQSIDHT
                     VNSKKRYQLPNIGNIILSKSSLIFNISNLPQFELSLKEFKEESSLKNFVKDSSNNQYE
                     ILVIIQDYSNLYRRIATPEYPLGILIKKNVAIIGLGSGGSLIALYLAKSGIGNLTLID
                     GDVIMDHNIVRHICSLKDVGRFKTLAVKDHILDRVPTIKITTVEKPFSRENKNIEAFF
                     DLLLSKVDLIVSATGDNIMNETINEFGYKRKIPIIFAGAFEKITGGIMIRIDPRKKSI
                     CYKCIYKKYGDDTIFQKPQIIDKNYIKNYGRDVKDMLTQPGLGIDIDLITLPTVKFIL
                     STLLEEKDIDQSFKSDIYYWFNKNLPDRKVETFVLYQEDRILEKIPDCHICSKIISKV
                     D"
     gene            10560..11030
                     /locus_tag="VF222_10920"
     CDS             10560..11030
                     /locus_tag="VF222_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142978.1"
                     /translation="MHESFNVVGNGDRSIQYEANEIAGAFELNSRWKFHIMDNGNTGL
                     VLELPVTQLQIEGSFEKIYFTFLIEYVHDYPLSPPKVHIIEPDLDPTQTPHMFIDNTM
                     CYLKPDEDWSSDYSSYDVGLMIKSWIFAYIKWKQKGVWGWGEHQEPQIEPSRSA"
     gene            11536..12726
                     /locus_tag="VF222_10925"
     CDS             11536..12726
                     /locus_tag="VF222_10925"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX7142979.1"
                     /translation="MDNNNQDKKNINEERHATWLELFYDLIFVVTISQLSHYLFHDVS
                     LSNFFGFLFLFIPVWWSWIGAAFFATRFYTDDIGHRLLLLLQMGGAGAMAVNISGAFS
                     NTFSGFALSYAFMRLILVLEYVRVFRTIKSSESTNPLVKRYIIGFSIAAIIWLISAFV
                     SVTEVRFALWVIGLIIDFATPNTVGKLHSKFAPHISHLPERMGLFTLIVLGESIVGIV
                     TGLTEQVWDVYSVIEASLGLCISFSLWWLYFDSTGRLPIQILREKGRISSYLIWLYTH
                     FPLVVAITAVGVGIRRLVSSDQHSALANSDLWLICGSVAISLISQSILQLISIDRDTH
                     DFTRRKKFTISRIISAIFIISIPILLNNYSIPIVIASLITGICGFQIVFDLKYHPHHR
                     IFKL"
     gene            complement(13013..14152)
                     /locus_tag="VF222_10930"
     CDS             complement(13013..14152)
                     /locus_tag="VF222_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HEX7142980.1"
                     /translation="MKAVVYKEPFKVAVEDVPRAKIQNPNDAIVKITTTAICGSDLHM
                     YEGRTAAKPGIVFGHENLGIVEEIGSGVTNIKVGDRVVMPFNIGCGHCRNCEEQYTGF
                     CTNVNPGFAGGAYGYVGMGPYMGGQAEYLRVPYADFNALKLPNNDNSNEQDFVLLADI
                     FPTGWGGVAQSGFKPGETIAIFGGGPVGLMAAYSAKLQGASKIFSVDGNKERLDKAKS
                     IGAIPINRHECDPVEEIINQTDGEGVDRGVDAVGYQASSTGGKNVPNVVLVSLVNVVR
                     PTGGIGSPGLYVTQDPGAPDESAKQGMMQFPIGKFFEKGLKMSTGQCNVKRYNRYLRD
                     LIISGVAKPSFVVSHEIKIDEAPIAYEKFDKRVEGYIKVMIHPQW"
     gene            complement(14305..14700)
                     /locus_tag="VF222_10935"
     CDS             complement(14305..14700)
                     /locus_tag="VF222_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7142981.1"
                     /translation="MITLNDAKKIIAAAENKAVEIGQPMNIAVVDNGGNLIAHERMDG
                     AWIGSIDISIKKAFTSRAFDISTKDLATQCQPGGQFYGIHVSNNGKVMIFAGGIPLKR
                     DGQVVGAIGVSGGSGEQDQSVAEAGAAKF"
     gene            15351..15488
                     /locus_tag="VF222_10940"
     CDS             15351..15488
                     /locus_tag="VF222_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142982.1"
                     /translation="MKKSIDIEEIIWEKADIEAKKRSISIDDLVKQALEVFLGMDTKK
                     I"
     gene            16060..16923
                     /locus_tag="VF222_10945"
     CDS             16060..16923
                     /locus_tag="VF222_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7142983.1"
                     /translation="MNYKFLKGKVTLISGPSGAGKSTLINIISGIISPSEGNIFLLEY
                     DLNTNFKKIKPYISCVWNETSLFSNMSVINNLRFFLFLRGQTQKETDIKINDILRSFE
                     IIHDKNKFISNLSLGTKRKIDLCRALINKFEILLLDEPSSFLDNKSKKILVKILCKYK
                     EEGKSIIIVSHHASLYSNLIDDIIIMKNGKIIYNQPLENLIQNTNEFRNAFILKTSDR
                     LLANDILQTLPEIENTYVSQKSVHIYLKEGEGDLSSIINSLGNRGLKIKKIISKEPNL
                     DKLIGEIINAH"
     gene            16913..17692
                     /locus_tag="VF222_10950"
     CDS             16913..17692
                     /locus_tag="VF222_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7142984.1"
                     /translation="MLIDAFILLTIKKIRNNLSFTLSTFTTILQPILWLSIFLYLREE
                     QITLNLNNNQIRYIDFLVLGIFVWRTVSFILGKFPSSIKEDASAIRSIISSPGGEKIL
                     IWSSLVATFSIAMIIGISILLTAVLLYDFSVFLNQIMIASLVFLLIIIIHFEIASIIV
                     ISHIHIKDMKSIGFITTTIFGLSAGVFFPVEFFPSPFDNFAYVLPLTQGLTIMREMLL
                     FPNLLPNINLLSMLLSETIILFIAAIVLYKKNFKSHWLEKL"
BASE COUNT         6814 a         2691 c         2648 g         6496 t
ORIGIN      
        1 aaagttgcaa atcttattgc tcttattttt ctatttaaga atttccatag ctttagattc
       61 tccttctttt attaatgctt taacagtatg aattgaaaaa tctgcatttt gaagaggata
      121 aggggattca gtagtccctc tactaattct tgtaatattt aaaatttttg caccgtagtt
      181 gttaacaagt tgttgatatt ctttttttat atattctaat ttctttgaat ctatttttga
      241 aaagttaagt tccgagtaca tattttcgat taaccctagt tgtcttgtaa ttagttttga
      301 tatttgactt aaactttcag acttatcaca atataatatg tctttaattc tagattgtac
      361 ttcagtcata tcggatggta gatgattaat ttttcttgaa tagttttcta ctataaagat
      421 atttttgtca taagatggag attgcaatag tacttctctc ataggggtat tacttaaaag
      481 agctccgtcc catccataaa ctccatcttc tacctctatc caagaaaaac cataattagg
      541 ataacctatt gacgctaata gatgttttaa ttgaatttgt attttaaaac tatcaaatgt
      601 tagaggttcc gcagtaagta catttacagc agttacaatc aagcgcattg ttttagtaga
      661 acaggacgat gaagtaattt ctggagatag ctttttaaag tctatatatt tttccaaagt
      721 cttgcctaaa ggagaattat catatagata ggtccaattc caaggaagtt gttgttcccc
      781 gaagagagat tcttttgtta tactattagt ttgaaaccat cttgggacaa acatttttgg
      841 aacaccatat attgctgcat ttaatgatgc cgagggtagt gtttttagtc ctattccttt
      901 acttttatcg tcataatcta aagtaacaat atctggaata acagtataac tactttcagc
      961 aagttccatc cagaaatctt ctaaatcttt taccggattg tcatttttac taccagctat
     1021 gatagcacca ttgatacctc caattgatgt tcctcctaca atatcaaatt taatattttt
     1081 tttatgtaaa attttgtaaa ctccacaagc aaaagcacct agtgaaccac ctccttgtaa
     1141 tactaaaaca ttctctatag gtttccttct ggatatggaa ttattgttat tattatgatt
     1201 attcatcttc aattgttaaa tattgttaat acttttatca tacctttatt tttgtcttgt
     1261 cgaagtttat tccactttta ttattattac tacagttgcc atcttttagg tcattgtcta
     1321 ttattttaga aatatcataa atattctaat cataaattca tcgtaaataa atgctaaagg
     1381 aaaagagttt acaatcaagt tatcgataat ctcatcagtt tttgaactca aaatatcatt
     1441 tattcaaacg atcaaatgat ccgatattta ataatttata gttttactat ctaaaataaa
     1501 ttctttatat cttattttac aatctttaca tttaagaagt atcatttgtt gacctgtatt
     1561 acgttctaat ttattgacat aaagcaaagc atttttgcaa aatgaacatt tcatccttaa
     1621 tttcaatgat attcaatcta aatgttatta gaaaatttat ttggataagt ttgatttaat
     1681 ttcctaaata gttaatatct ttagagttat tattgcttac tgaaacacta tctgtattgt
     1741 aaattcttac agaaaaaata tgccttactc aagggatact ctttttatca taactaaatt
     1801 tcaacctata gaatattgtc ctataatttt cttaaccttt cctttggcaa attaaaataa
     1861 taaatcaatt attcaatgta cttaccggat aaactccagc agaaaaatgc ggcattcaga
     1921 tttaaggtaa tgacaaatgg ctaattatta ttcaaaatgc aaaccgtcgg ataaccaata
     1981 taaaaactta aagtgacaga cttgtatcaa cagtttttta ttttccattc tcttattaca
     2041 catgttcaag cattgtcttg agcttcaatt acatgggtgt taggggataa ctttaacacc
     2101 cattttaatc acctttaaaa tcaacagaac ctattttttt ttgatatttt attttttata
     2161 aaaactttat agttaatcct tcataaggaa caatacaatg agataactag taaattttaa
     2221 tttgtattat acaaatattt gccgtattat tagatacaaa caacgctact tggaagaaca
     2281 gccctcaaat aattgaagca aacaaatttt atgtcatact ataagtgtaa aaacatacaa
     2341 tatataattg ttcaagcgat cttaatatct ttaaaccatg aatgtcggaa acattattat
     2401 tggttcttat tttatataaa ttaattagtt attattttcc acggtagttt gttgtaataa
     2461 ttctccaatt tcattatgta ttttaagaaa gaacaatcct ttttctgtag tcttgaattt
     2521 gtgagtaccg tcaagatatt ctattaggtt gttttctatc aagctagtta gatattcgtt
     2581 taattggtta taacttagaa aagctgtgta cataatcttc gtcttggacg ctcctccatt
     2641 cgcagcatcc agtatacttc ccactatttc tgtcctactt ctatttccca tattattaga
     2701 taagtgattt acttatttta gtattaatat cacaagaaaa gttaagataa ttatagtaaa
     2761 acattccaca tattatgtac aaataaaaaa tataagaatc atgtcttatc tcaaaattga
     2821 gttttcctta tgttagagat tggataaatc aacgtcattt caagaatacc actgatgctt
     2881 atccaatatg taatttgatg atagaagctc ctgtaaaacc gtaagctatg tggattatga
     2941 tgaatcaatt aagtattaga atcctccaaa tactagaata agatccttta aagatgtcaa
     3001 agagaaggag aagcttgaat attcgataaa aacgacgaaa tggaatcctt cttattgttt
     3061 acgacacaac tctataagtt ccgatagcga ttttctacca gaattatgcc ttagaaaaaa
     3121 gtaagatgga atgtgaattt aaagcaacag gtaatggaaa attgctacca gcatagataa
     3181 agcaaccatc tataataatc caaatatttt aacaaactct caaaatctaa ataatttaaa
     3241 tcagttattg gtgactaaca aagggaatga tatattggta cagtgatcta attaacattt
     3301 gcttatcaaa aacaggataa aaaaagtatt atgtatatat aaatttattt cattaatatt
     3361 ttacccaaat aattgattga agctacaaat aaatataatt gataaaattt actgtataat
     3421 attaagtaag aatctttcaa tatctcttta tttacaagtt ctttttttac tatacaagaa
     3481 aaatgttaag aatcaataaa aaaattctaa attttaataa tatgcaagtc tccaaaatac
     3541 ctaatccaaa ctgtctaatt ctttccctat tttgtgtaat aggattatta tttttactag
     3601 ttcttgaaac tgattttgct ggtaataaat ataatatcat gaaatcggta tgggctgatg
     3661 aaattaatgg gactgaaaat gctgatagta ttacgggtac attaagtcaa gatactatca
     3721 aaggtttaca gggaaatgat actataggtg gcaaagaggc aggtgatgac ataagtggag
     3781 gtgatggaga tgatgcactt tatggaaatg aaggaagaga ttggttacga ggtggaagtg
     3841 ggaatgacca tttagaagct ggagaaggaa atgatatgct atttggtgat agaggagacg
     3901 atacactata tggaggtcct ggaaacgata agcttactgg gggaccagga aaaaatatgt
     3961 tcatctgcgg gccaggtcca gatatggtaa tagattttaa tgttacacaa caagattcag
     4021 ctcctgagaa cgactgtgaa aatttaaaaa ataacactac aaatgtcaat agtacctcat
     4081 ccctcaacaa caacacctaa acgaaaatgc tggaaatata aatagcaatg aacttgttga
     4141 tgatgctact aatagaacaa ttgataaaaa caacaatcca gataaaggat tattttttgg
     4201 ctggtttaaa taaatagatc taaataatag atttttttaa tgaggggagt tagaaattat
     4261 caataacaga ttgaatcatt tgcgataatc tcggatagga tcggtgataa ttaatgtatt
     4321 gtctgtttta aaatctttca caatatacta ctgaaattct tcaacaattt agtaatttcc
     4381 atctatgtaa tatttgcatt tgtttcaaat tattatgatt tattcacaaa aaacaattaa
     4441 ttaattaatt aaatacagaa ttacaagatt ataataatta tgtttttgat tgttataatt
     4501 acgtttgaaa attagttgca tcgcttatcc acactggaaa ttgtttataa ttagacttta
     4561 aaggattaga gaaagaagtg agtataggag caacacttcg aaattttata aaagtaatta
     4621 agttattatg aataaaactc tactaatgaa gattaataga atctatgtag tatattattc
     4681 ttttacaagt atttataata atgtttacac attttatcag tagagaaacc aaacaaagtt
     4741 ttttaactac attttaaaat ataacacgat gcttttagaa tttaaatggt atgacttaat
     4801 aaaattttct tttattttca gtattgtctt ctttcttatt tgtttacaaa ctttaacttt
     4861 tcaagttgtt ttttctacaa ataataataa atctttcgaa tttacaaatg aaagtaatct
     4921 tactactaat actttcaaaa aaaattttaa taatataagt gatggtgatg ataataatac
     4981 ttctcctctt aatcaaaatc aaactttaaa tgaacaaaaa ataaaaattg ttgctgcagg
     5041 tgattttggt tgtcgacctg ttgcgcaaaa caatattaaa caaattgaat tggaaaagcc
     5101 agatattttc ttggtattag gagatttatc atatgaacca actatggatt gctggtatga
     5161 tatgactaaa gggttagatt caagaattaa aattgcgatt ggaaatcatg aggatgaaga
     5221 agaaaaagca aaaggaggtt ctaaagaatt aaaagattca ttgttaaaac attataattt
     5281 acaaaattca tattattcat tcgattatag aaatatacac tttttagtat tagatacgca
     5341 attagaattt tcatttgatg tttttaaaat gttggaggaa gaagaagaaa aggctcgtat
     5401 tgaagagacg agtaacaata aagatgatga aagtaataaa ccagaggcaa aatattatgc
     5461 tacaacactc aaagatctat tgaccaaata taatataaag ggcgaaatac ccccatatca
     5521 tttaataaat gatgaagtaa ttgttgaaga tgttccactg aatacagaac aatataaatt
     5581 tgctgtaaaa gatctagaga aggctaataa taactcctct attgattgga tatttgttat
     5641 gtttcacaag ccattttatt cttcacttac aagccatatg caagaatata taatgagaga
     5701 aaaatatcaa cctgtatttg acaaatatgg tgttgatcta gtattacaag gccataatca
     5761 tctatacgat agaactttac ctcttaggtt taatcctcag aatattagca aaccaattgt
     5821 agatgaatca aataatacaa ataaatttgt caatccagaa ggctcaattt tctcagttat
     5881 aggtctaggt ggcagaagca gtcatatatt tttaaatcaa ccagattatg ttgtaaaaca
     5941 gtctaatggg tttggattcc ttagtatcga gattaatgga aaagagttag atgcaaaata
     6001 ttatgatata ggatacaaat gcaatgaaca aaaattaaaa gaaaaagatt tagaagaaga
     6061 aggagatttt ataatatatg acatgtcatc gtgtaagaat gataataaaa gtaaagactc
     6121 attaaaaata attgaccatt acagtatatc caaagtaagt taattaattt tatcaaaact
     6181 tttttcttat attattgttt ctcattattt attcaaaaaa ttttcaagaa tatatagaaa
     6241 cacaatattt taaaaattaa ttgtttaaga atcatttcta gtcagtagac tattaaatta
     6301 taccaatttt attttataat tgaatttatt gtggtatata gcatttctta aaacacataa
     6361 tgaaattgaa tctaataaca atagtgtata ttgttataca aaagttcgat cttaagatat
     6421 agttataata taaagtaatg gttaattact tgtttctaat ttaatattat gagtccattt
     6481 ttagaatcaa tttcacaata taaaacattg gtcccattct ataagttggt tcatataatt
     6541 gattttgata atatattttg tgaaagcggt atggagaaaa gaaggataaa tgccattaaa
     6601 actatctaca acaatagaaa aaatttaaaa tcttcctata tcaagaatat agaggtcata
     6661 aatgaacttc tcgaatctat gagtcgaata tatgagaagt aatagttctt cagaaaatca
     6721 tcaaaataat aatttaaaag ttgtaatagc atttagtaat tttttttata tctcgtctat
     6781 cgaacgtagt ccactatctt cttgtataac ctcatctaca taatgtgtat gaatatcccc
     6841 atcatcataa atgtcaccga ctgggatata attttgtcct tcatcatcgg gtatttctga
     6901 tgatacagtc gccattagat ctgagcgaac cggagagata gtaggtaata attgcttaat
     6961 aggcaaaggt atatcatcat ccatttcatc ttgatagtaa taaagattca aagcaaagta
     7021 tctaccattt atattcccta attctattac ggtaggttta gattctttgg atttttctaa
     7081 attagatata atagaaaata ttttgttata atttagctca atagcttttg ctaacttatc
     7141 tttatctcct tccaaaaacg gtttgtttaa tttattagct aattgtatga atgagtcttc
     7201 caatcttatt atcatacatt tctacatcat tttaaactaa tgatctatat gtatgctctg
     7261 ttttttcctg tgtctttttt taaatctatt tgttatagat tatataaata ccaatcaatg
     7321 atgctgtcac agaaattatt tcagaagaaa gacacaaaga attgactctc ttaaactaat
     7381 gttcatatat gcaattaaaa tcgagctcac aacaaaatat tagtagaaag attgaaaaga
     7441 ttttttaaaa cttttactag taaagaaatt tgttacacta ctaaagatac agaagtaata
     7501 gtggcattat ctatagaaag tagagaaaaa gtagatgata tgataaacaa ggtaatagaa
     7561 gcaggaggta aggaagatag agagccacaa gatcagggat ggatgtacag tcgtagtttt
     7621 gaggatattg atggacatct ttgggaaata atatacatgg atgaaagtac agtaacaaaa
     7681 taataaaata ttacttcaca ctaaatgaat aacaagtatt aaaaaaagaa aaaaatttag
     7741 gttatagata tataatgatg atcaactttt gaattgttct tactatattt ttttgtctct
     7801 gtaatatatt tacaactgtt tctttatttt taacagtttc tggactgcca ttacatcaga
     7861 aggattagca aaaaagacta actaatgagc tctatattcg agaatgactg gcatatgagc
     7921 cgcttgagga ttttaaatga tatatttata cacaaaatag tcagtatgct aaagtcttaa
     7981 gactgtatcc aatctcctac cactatattc atagcaccat cgttattcaa taaacctctt
     8041 gtagaattta taaagccaat attacacagt gattatctcc caatatctta ctaaattcgt
     8101 atctgttggt tattatggtg atggtttcta caaagccaag taatagaaaa ataataaata
     8161 tatcttatta ctatatacta atgatgccta gagatttgaa acatgtaatt gaaggtttag
     8221 aagggataaa aaataaagct acagaggtag ttcttagaag aaaagaagaa gataatatgg
     8281 ttgatgtagg gactggaaaa gtaactatcc ctaacataaa gcatttttat ctattaaatg
     8341 atttataaaa agttaattat atcagaagat gtaattaatg atatagttga agaaagtaat
     8401 agacaccaaa atgttgaaac aggtggatta ctttatggaa agctcatcgg agaatatatc
     8461 ataatcttaa aattaataca cttttcatct aatgcaagaa gaacacaagt ctattttgaa
     8521 atggatgaag attttgcaat aaatattaca aaacaaatgg aaaaaagtaa tcttgcttat
     8581 ctaggaaatt ggcataaaca tttaggatat ggaggaccaa gtaatggaga tgataaacaa
     8641 gctgagttat ttcttataca gaatagtcat aaacgaaatt atttatccct aattatagat
     8701 ttttataata atgattataa cctgatagca actaattact attttgataa agctcaattt
     8761 cttaaaaagg atattcagat tcagagaata attaacaaac aagaattaaa caaaatgttt
     8821 aatgcgaaaa cagtaaacat taggaatttc attgataatc tatcaaaaaa tttagaagaa
     8881 catactggta aaacatttat ccattcacaa tcgaataaat cacaaaatta taaattattt
     8941 tatgaggaaa tttataatcc gcaagataat ggatccattt caactaataa aattgtctat
     9001 tctgtattta ttaagtttcc atatctcaat atatctatga ctgataagat tagtattgga
     9061 gttactttag atcataaatt tactagaata tttgaagaaa ttacattttc agatattttt
     9121 tttaatatag tagaattatt acccaaaata aaaaatttgt ttgataaatc acaatattat
     9181 attgatatat cttcatattc atataataag aaagaaaatg gtgacttgaa aagtagaaca
     9241 aaaacctttt ttagagcttt aaaatcttta tctatattta cttttcattt aaaggagaaa
     9301 gttaaaaacg tcaattaggg aaaaacataa cttaacatta aataagaata gaacaaacac
     9361 aaaaactgct tttcttgtga tatttcctga agttaaacaa acattattag ataataagaa
     9421 atttaaaagt aatgaaaaat ttctattttc cgttaatgga tctatcatgg atgactcaac
     9481 atatcaaata caaagtattg atcataccgt caacagcaaa aaacgatatc agttacctaa
     9541 tataggtaat ataatattat ctaagtcgag tttaattttt aatatatcaa atctcccaca
     9601 atttgaatta tctttaaaag aatttaaaga agaaagctct ttaaaaaatt ttgtcaaaga
     9661 tagcagtaat aatcaatacg aaatattagt cattatacaa gattattcga atttatatcg
     9721 tagaattgct actcctgaat atccattagg aatacttatt aaaaaaaatg ttgctataat
     9781 tggtctaggt tcaggtggtt cattaatagc attatatcta gctaagagtg ggattggaaa
     9841 tttaacttta atagacggtg atgttataat ggatcacaac atagttaggc atatttgttc
     9901 cttgaaggat gttggacgat tcaaaacttt agcagttaaa gatcatattc tagacagagt
     9961 tcctacaatt aaaattacta cagttgaaaa acctttttct agagaaaata aaaatataga
    10021 agcctttttt gatttgttat tatctaaggt tgatcttata gtttccgcta caggagataa
    10081 tatcatgaat gaaaccatta atgaatttgg ttacaaaaga aagataccga ttatatttgc
    10141 tggtgcgttt gaaaaaatta ctggaggaat aatgataaga attgacccac gtaaaaaatc
    10201 tatatgttat aagtgcatat ataagaaata cggcgatgac acaatatttc aaaagccaca
    10261 aattatagat aaaaattaca ttaaaaatta cggaagagat gtgaaggata tgttgacaca
    10321 accaggactt ggaatagata ttgatctcat tacattacca acagtaaagt ttattttgag
    10381 tactttatta gaagaaaaag acatagacca atcattcaaa tctgatattt attattggtt
    10441 taataagaat ctaccagatc gtaaagtaga aacgtttgtc ctatatcagg aagatcgtat
    10501 attagaaaag atacccgact gccatatatg ttcaaaaatc atttcaaagg tggattaact
    10561 tgcacgaatc ttttaatgta gtaggaaacg gcgataggag tatacaatac gaggcaaatg
    10621 agatagcagg ggcattcgaa ttgaatagca gatggaagtt tcacataatg gataatggta
    10681 atactggatt agtacttgaa ttacctgtga cacaacttca aattgaagga agctttgaaa
    10741 aaatatactt tacttttcta atagaatatg tacatgatta tcctttgtct cctcctaaag
    10801 tacatataat tgaacctgat cttgatccaa cacaaacacc tcatatgttc atagataata
    10861 ctatgtgtta tctaaaacct gacgaagatt ggtccagtga ctatagctca tatgatgtag
    10921 gattaatgat aaagtcttgg atatttgctt atataaaatg gaaacagaag ggagtatggg
    10981 gctggggaga acatcaagaa cctcaaattg aaccatctag atcagcctaa agcccaaata
    11041 tgatttaatt ttaaagtatc tttattttat cttgataaat attcattctc tccgaaaaaa
    11101 ccttaaatat ttcctctatt cttcgcgtct cttaaatcat agatgtaaac tgaagtaact
    11161 ccattttttt attttaataa cagagagaat tattatttaa ttacatttca tacccaagac
    11221 ataaattaaa aaaaagattg tctaacagtc taaaataatt tttgttttaa atagttggcc
    11281 tcaaatatgt tagtctctca caaccatcct taatgacgat tgcacttaag agtttgtggt
    11341 aaatatatac aagaatgcaa aataaaataa cgcccacaat taggtcaaat ttggtttaca
    11401 tcccactggc cccatatagt ataaaagggg acagtaaatg tttatttaat tatatatcac
    11461 agtaactgta aactttgatg acctactaca tatcatctat atttattaat tttgaattat
    11521 gctatttaag ttaacatgga taataataat caagataaaa aaaatataaa tgaagaacgt
    11581 catgcaacat ggttagaact tttctatgac cttatttttg tagtaacaat ttcacaacta
    11641 tctcattatt tattccatga tgtgtcttta tcaaattttt ttgggttctt gtttctcttt
    11701 ataccagttt ggtggtcatg gataggtgca gcattctttg ctactaggtt ttacactgac
    11761 gatataggac ataggctttt attgctatta caaatggggg gagctggtgc catggctgta
    11821 aacatttcag gtgcatttag taatacattt tctggttttg cgttatctta cgcatttatg
    11881 cgtttaatac ttgtattaga atatgtccgt gtattcagaa ctattaaatc ttcagaatct
    11941 actaatcctt tggtaaaaag atacataatt ggtttctcta ttgcagcaat catttggtta
    12001 atttctgctt ttgtgtctgt tacagaagta cgttttgcat tatgggtcat tggattaata
    12061 attgattttg ctactcctaa tacagttggg aaattacatt caaaatttgc acctcatatt
    12121 tctcatttac cagaacgcat gggcttgttt acgcttattg tactgggaga atcaattgtt
    12181 ggtatagtta ctgggttgac tgaacaagtg tgggatgtat attcagtgat tgaggcatct
    12241 ttgggattat gtatatcttt tagcctttgg tggttatatt ttgacagcac cggaagatta
    12301 cctatacaaa ttctcagaga aaagggaagg atttcctcat acttaatctg gttatataca
    12361 cactttcctt tagttgttgc aataacggct gtaggagttg gcattagacg tctagtatca
    12421 agtgatcaac attctgcatt agctaactct gatttatggc taatttgtgg ttcagtagcg
    12481 atatctttaa tttctcagag tatattacaa ttgatttcta tagatcgaga tactcatgat
    12541 tttactcgta gaaaaaagtt tacaatctcc aggataattt cagctatctt catcattagt
    12601 attcccattc tattaaataa ttatagcata ccaattgtaa tagcctcttt aatcactgga
    12661 atatgtggat ttcaaatcgt ctttgattta aaatatcatc ctcatcatag aatttttaaa
    12721 ttgtagatat gtttcttttg acatgaataa attataaaaa tatatttgta agttctgtca
    12781 atagttataa aacaaatagg attggtaaca agattgtgac ctttagccca agaggtttct
    12841 gtaacttgct ttttattttc attattgtta taaattgtct cttatatatg tggatattta
    12901 ttttttttta agtataattt attagacaca aaatattatt tcttgctaca acaaatatca
    12961 aacaatcatc aacaaagaaa attttgaaaa gttaattaga tttagatcta tttcaccatt
    13021 gtggatgaat cataaccttt atgtagcctt caactctttt atcgaatttt tcataagcaa
    13081 tgggtgcctc atctatcttg atctcatgtg atacaacaaa gcttggcttt gctactcctg
    13141 atattatgag atcacgtaga tacctgttgt aacgtttgac attacattgt cctgtactca
    13201 ttttgagtcc cttctcaaaa aatttgccta ttggaaattg catcatacct tgttttgcgc
    13261 tctcatcagg tgcacctggg tcttgtgtaa catacaatcc aggagatcct attccaccag
    13321 ttggtctcac aacattgaca agagacacaa ggacaacatt tggtacattc tttccacctg
    13381 ttgaagaggc ttgataacct acagcatcta cacctctgtc cacaccctca ccatctgtct
    13441 gatttattat ttcctctaca gggtcacact cgtgtcgatt tataggtatc gctcctatag
    13501 atttggcttt atcgagtctt tccttatttc cgtctactga gaaaattttg gaggccccct
    13561 gcaatttagc actgtaagca gccattaatc ctactggtcc accaccaaat attgctattg
    13621 tttctccggg cttgaacccg gactgtgcaa caccacccca tccagtagga aatatatcgg
    13681 caagtaaaac aaagtcttgt tcatttgaat tatcattatt gggtagtttt agtgcattaa
    13741 aatcagcata aggaacacgt aaatattctg cttgtcctcc catgtagggt cccattccaa
    13801 catagccata tgcaccacct gcaaatccag ggttcacatt tgtacagaag cccgtatact
    13861 gttcttcaca gtttcggcaa tgaccacaac caatattgaa aggcattact actctatcac
    13921 ctacttttat attcgtaaca ccagaaccaa tctcttccac aatacccaga ttctcatggc
    13981 caaaaacaat tccgggtttg gctgctgttc taccttcata catgtgcagg tcagagccac
    14041 atattgcagt agtggtaatt tttacaattg catcattagg attttgtatc tttgccctag
    14101 gaacatcttc tactgcaacc ttaaatggtt ctttgtatac tactgccttc atttcaatta
    14161 ctatcagata atataatcac caacaaaact tttatctctt ctagtaaaat gtattaaaac
    14221 ataaaaaaaa ttttatgata tgttttaaat attaattatt acaccaattt gagctgaaat
    14281 ctttcaatgc atatacccat atacttaaaa ttttgccgca cctgcttcag ccaccgattg
    14341 atcctgttca ccagaacctc cgctcacacc aattgcgcca acaacttggc catctctttt
    14401 gagtggaata cctccagcaa aaatcataac tttgccattg ttggaaacat gtattccata
    14461 aaactggccg ccaggctggc attgagttgc taaatccttt gtagaaatat caaaagctct
    14521 tgaagtaaat gctttcttaa tagaaatatc aatactacca atccaggcac cgtccatacg
    14581 ctcatgagct attagattac caccattgtc tactactgca atattcatag gttggccaat
    14641 ttcaactgcc ttattctctg ctgcagcaat gattttttta gcatcgttga gcgtgatcat
    14701 gacaataatc tccctctata aaaaatctta aagtatacct ttcccataat gtaccatagt
    14761 aaacaaaata attaacgatt tgagaactta tattatatgt tacctattta ttcattgatt
    14821 gaaaatacaa aacgaattag tttacttaag ataaattatt ctactatgga aaaattctca
    14881 ttgtcataat tgatttcgaa ttttataata ttgtattaaa ctttttacaa actagtgtag
    14941 taaatcaact acctctatac aatcttaata tgagcataat aggggtattt gaaagacatt
    15001 tgaaaactat ttgaacgtta ttaaataatc ataaaataat tacgtgaaat gcatttgaaa
    15061 ggtatttgaa atgatgaaaa tataatttta atggtggctc aaacctcgaa tgttattact
    15121 tgtttattat tgatcatcat tcttaatcta tcatctgtta aattatacca tctccaagaa
    15181 ttaacaaatt ttttcctaga tgaattaaaa acatctgact gatttaagaa attaagaatt
    15241 tgttataaag tgagatcagt gattgactaa gactaagtta ttccattatt aacataatca
    15301 caactctaat atagtaatat tatagtaaaa taaaaaagga tacttttaaa atgaaaaaat
    15361 ccatagatat tgaagaaata atttgggaaa aggctgatat tgaagcaaaa aaacgtagta
    15421 tatctataga tgatctagta aaacaagcat tggaggtatt cctgggcatg gatacaaaaa
    15481 agatatgatt gaagaataaa ctaatactaa aacaataaat ctattttaag aaattcactt
    15541 ttgaagtctt atagcttggg aaacaaagag aatttagtaa aaccaacact tttagacgtt
    15601 aatgtacttt gtggtttttt taattattct acttcagtaa tatatacgat tatcaaaagt
    15661 taatttgaat aggttaaatg taagaaaatt gaatctgcat tctttttttt tcattttctt
    15721 ttttagatga ttttctttat ggtaaaataa ttttataaat gttcatatat acctctcctc
    15781 ttatacaata tccatcatat gtaaacatat gatatcgcga tataataaat caggttactg
    15841 tgatttaggt aacaccgccc acaaagcggc cttcgctaca aaaccgccca caaagcggct
    15901 tcccaaaagg aactacctta accacagaac ctgatcaatt atatcaacca aaaactgaat
    15961 gaatttatag acaaatttta cataatctca gtcatatatc cagtcattga atgtaacaat
    16021 ataggaaaaa aatatcataa tcattgggcg ataaagaata taaattataa atttttgaaa
    16081 gggaaagtaa cactaatatc tggaccatca ggtgcaggta aatcaacttt aataaatatc
    16141 atttcaggta ttatttctcc ttctgaggga aatattttct tattagaata cgatttaaat
    16201 acaaatttca aaaaaattaa accttatata agctgtgttt ggaatgaaac ttcgctcttt
    16261 tccaatatgt cagttattaa taatttacgc ttttttttat ttctaagagg acagactcag
    16321 aaggagacag atataaaaat aaacgatata ttacgcagtt ttgaaattat tcatgataaa
    16381 aataaattta ttagtaattt gtctctagga actaaacgaa agattgatct ttgtagagcg
    16441 ttaataaata agtttgaaat tcttttgttg gacgagccat catctttctt ggataataaa
    16501 tcaaaaaaaa tattagtaaa aatactttgt aaatataaag aagaaggaaa atcaatcata
    16561 attgtttctc atcatgcatc tctatattct aatttaattg acgatataat tattatgaaa
    16621 aatggaaaaa ttatatataa ccagccacta gaaaatttga ttcagaatac aaacgaattc
    16681 agaaatgcat ttattctaaa aacatccgat agattgctgg caaatgacat tctacaaact
    16741 ttgcctgaaa ttgaaaatac ctatgtaagt cagaaatcag ttcatattta tttgaaagaa
    16801 ggtgaggggg atttatcttc cattatcaac tcattaggca atagaggatt aaagataaag
    16861 aaaatcatta gtaaagaacc aaatcttgat aaattgattg gagaaataat taatgctcat
    16921 tgatgcattc atattactta ccatcaaaaa gataagaaat aatctttcgt ttacactaag
    16981 cacttttact acaatactgc aacctatctt atggttaagt atttttttat acttgagaga
    17041 ggaacaaata acattaaatc taaacaataa ccaaattagg tatatcgatt ttctagttct
    17101 gggaattttt gtttggcgga cagtaagttt tattctaggc aaattcccaa gttcgataaa
    17161 agaagacgcg tctgccatcc gaagcataat atcctcacca ggaggagaaa agatattaat
    17221 atggtcaagt cttgttgcca cattttctat tgcaatgatt attggtatca gcatattact
    17281 aactgcagtt ttactctatg atttttcagt tttccttaat caaatcatga ttgcttctct
    17341 agtatttttg ttgataataa ttattcattt tgaaatagca agtattattg ttatttctca
    17401 tatacatata aaggatatga aaagcatagg atttataacg actaccatat ttggattatc
    17461 agctggtgta ttttttcctg tagaattttt tccatcccct tttgataatt ttgcttatgt
    17521 tcttccatta actcaaggat taacaatcat gagagaaatg ttgctttttc ctaacctttt
    17581 acccaacatt aatttattga gtatgctctt atctgagacc atcattcttt tcatagcagc
    17641 aattgtttta tataagaaaa attttaaatc tcattggctt gagaaattat gatggtaaat
    17701 aaaatagaga atttaaatca tagtattacc taacaggcat ttaatatcaa ttatagatca
    17761 attgccatta ccttaggaat ccttcttata gcagtacatc caaaggttaa attaattgat
    17821 aataatatga tatacattct gtttcctaaa gaaataaact tcttatcctt gatataaaac
    17881 ggaataatct tgattattat gtaactactc ctaattactt aaaatttaaa ctttgagagt
    17941 tttaacaata ttttttagat aattaatttt attcatagaa aagattaaat attttccgaa
    18001 aaaaagagca ccacaattag gtaaaatttg tttcacatcc aactgatata gatacaatgt
    18061 tttaaatcct attaattaat aacaaaaata gttcttaaca gagacaatat tactttacct
    18121 aactactatt tttttcttat cttcataaac ttaaaataaa ttcatataat ataaatataa
    18181 tttcgaatct tcccatttca caatttagag atagaatcca aatctataga gttttttcct
    18241 gttgaaagcc agtatctact gtgattacat tttatacgga catgtgaggg gttgtatata
    18301 atgctggaca aaaaagaatt aaatgcaaag tgaataaata cagcttgtgt agaatcagca
    18361 aaaaaaaact ttactagtca cattgtataa aattaagatg atttctatat acagaacatg
    18421 attatgcaca gatatcttta cattttttta tataccttgt aacgtatttg ttactatgtc
    18481 aacaaaagaa gataaaatta atgaaacaaa cataaataga actaaattaa acacagacaa
    18541 cactaatacc accaatttca atgaaagaac aaatagagac tacaataaca ccttaaatca
    18601 acaacaagat gcaataaaca agacactaga caacacctta cagaatgta
//
LOCUS       DASVEV010000251         6719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_708456, whole genome shotgun sequence.
ACCESSION   DASVEV010000251 DASVEV010000000
VERSION     DASVEV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708456"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            494..748
                     /locus_tag="VF222_10955"
     CDS             494..748
                     /locus_tag="VF222_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142985.1"
                     /translation="MQKEKINRPFFKSITYELRIQKIREWQNKVIGRLLFLFRQNRRN
                     EFLSRKTIAYVFSGFSIIFIGYVLMNILTNYPPIEIGISP"
     gene            complement(1624..1770)
                     /locus_tag="VF222_10960"
     CDS             complement(1624..1770)
                     /locus_tag="VF222_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142986.1"
                     /translation="MAISTKDNNTLKKKGSSLGNMLKGTGIFIGIFIVVYIVIIFATG
                     GFSK"
     gene            2070..2264
                     /locus_tag="VF222_10965"
     CDS             2070..2264
                     /locus_tag="VF222_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142987.1"
                     /translation="MRWANKLAGYFFGALLVLFGFLWIASTMTIPVIFIGSIGLIIVF
                     GGFAIIYLGHRSGRQKKLLQ"
     gene            complement(3026..3631)
                     /locus_tag="VF222_10970"
     CDS             complement(3026..3631)
                     /locus_tag="VF222_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7142988.1"
                     /translation="MQYIIIQIAYSQIDNSSSPNNITNNTLSPPSIVKPKVDLKIEGT
                     IIDDKFKGGDGNDELNGKEGDDQLNGGKGDDELDGDEGNDLIKGQQGNDIIEGDEGND
                     KLIGERDIDVITGAEGDDNIDGGKGDDHLDGGKGSDEINGGEGSDIMIGGLGLDTFIC
                     DEFDKIMDFGSTEGDKKIGSCLVINYIKSNTTQLLGNTTMQ"
     gene            complement(4130..4996)
                     /locus_tag="VF222_10975"
     CDS             complement(4130..4996)
                     /locus_tag="VF222_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7142989.1"
                     /translation="MSEGPEVKIVADKISSSLSEKKIEDIIFKNLNIDIKNKIIGSKL
                     KKIKTFGKNIVIQFSSGIYLRNHMMMWGKWRIYDRHKYDNGLAIPPERSKWKKNPVLV
                     KNVKDVREDSRVRLTIITKETVLVEFNGPILQFSIDNPAEKEPIKSLGPDCLGDIFDI
                     DEAKKRLQTKQQLLISEALLDQKIISGIGNKYKSEILFICKIWPFKQISQLETTEENI
                     LFEGIPQLLRYGYKNSGRTRHLLKNENSSWNTRHWVFRRSGKECWICKSMIKSEKTLT
                     SRATFWCPNCQQ"
     gene            complement(5184..6089)
                     /locus_tag="VF222_10980"
     CDS             complement(5184..6089)
                     /locus_tag="VF222_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142990.1"
                     /translation="MHNTQKRVNWKNDVIPIIKERLALFNRYGIIPTFKTIFYSLVFL
                     NIIPNSYNYYKYLSKYVNHAKIKGDLPIGCFPEQDLPRITNSYPEFVNAKEYIQKIVN
                     YLEETSANPSIQDYKFNNKSNYIYTDIEIWVEKNAPSFTLHSLLKNSGVKIISNKRTN
                     NLEFINKNIKRLKCSFKDDKKIYILYFGNFDLFNNNIDKIINNTLMDFNLNIEFNRIA
                     VTENQMRKFHLPENPNPEIIRKLDSNLGKDYFIRKYGRLFQIELESFQAYAPEKFKNI
                     VIESVNNILNKYRNCENIKNIHLMA"
     gene            6158..6514
                     /locus_tag="VF222_10985"
     CDS             6158..6514
                     /locus_tag="VF222_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142991.1"
                     /translation="MPQPGQRTITFSGKVLKKLEEIYILEKNKNPSLSFASFVSESAL
                     IELERRRILKEAPLISLISLNDDIIILKDLRKNNRLIEVQIRNKNLKCITDDKFDCIH
                     VGFTLALPEVRKALSK"
BASE COUNT         2375 a          930 c          846 g         2568 t
ORIGIN      
        1 aagataattt atttaattgt catagcctac ttcacaagga atagaacagt tactatttta
       61 ttgtattgta atagcaggct atgatggaca ttgaattgtt aaggaattat taatatgagg
      121 cattattaat atgaaatatt ctatttaact ttgagacgat ttagttttct tttggtattg
      181 ctttgcttct ttggttattt gaaataaatt atagcgctct caattgtggt tgaggatggc
      241 ccagactgtg acaatcaagt tttcatttga tcagaactga atgagagtaa cgaatgatag
      301 cagtattatc tcttcgcaat tctcattata aattatgaag aggtttctaa aagatgtact
      361 cttttacagt tacattagca attataatac gtgatctccc tctgtaatat tcaaaaccat
      421 caatttacca ttttataatt aatatatata aagtttaaaa taacgcagtt gcatataaag
      481 atcaataaat attatgcaaa aggaaaaaat aaatagacct ttttttaaat ccattacata
      541 tgagttaaga attcagaaaa tacgagaatg gcagaataaa gttattggaa gacttctttt
      601 cttattcaga caaaatagac gcaatgaatt tttatcaaga aaaactattg cttatgtttt
      661 ttcaggattt tcaattatat ttataggata tgtattaatg aatattttaa ctaactatcc
      721 tcccatagaa atagggatta gtccttaaat taaggcatta atattcaaat aaaaattatt
      781 ctaatttcta aaattagtta ctctttattg ttatttgtta ttattgttat cattattatt
      841 attcacttct attcattctc aaaaagttta tttgtatatt atttaaaatt ttttttaaca
      901 attaaggcta tagcctgaga aacaaaataa aaattaatta ctttgctaaa ccttgtaatc
      961 ctttgccaat agtttcagcc ggaccctttg cacctttagg aatgtttata tcatttcagc
     1021 taataatatg tatttaatat tattattatt attaataaca tcatcactca gtttattata
     1081 ctatctatga agaagtctta ttcctgtata aatgaataga attatcgtca tcatctatct
     1141 taatctataa tattgaccgg ataattgtgt cttgaatgtt ggcttagggt cacaatcttg
     1201 ttaataatac taacctaatt aaaatatatt aaattacaaa aactaaagcc atagtcctcg
     1261 atgagccaaa gatatataga agacaatgtt aggcagaagt tctttggaat gactggtttt
     1321 acgaagtaaa caccattgtt aaatatgcta tttaatagtt gaagataatt taaatgccat
     1381 agcctgcaac ttgtatacca ctagaggtga gtgaggagcg aacagaaatg gtatcctaaa
     1441 agtagaagta gtaggctatg gcatatctat gtcttttcac ttgttgtagt gtaatatcat
     1501 agagatttta gaatattgat tatttgatta acaatgttaa ttaaatttta ggagatgtat
     1561 atctaaataa taataataat aataaattaa cactctatga tcataaaaaa aggaaaatat
     1621 attttattta ctgaatcctc cagtcgcaaa tattataact atataaacta caataaagat
     1681 accgataaat atacctgtac ctttaagcat atttcctaat gaagaccctt ttttctttaa
     1741 agtattattg tctttggttg atatagccat tatattatat tataaactca tagtaataaa
     1801 gatataatat gctaaaatta ataattacta aaaaaattga agttctgagg ctattaaata
     1861 tactcttaat cgttccaatc tctctatttg atactgataa tagaataagt tgatcaatct
     1921 tttgaattag agtattcgtt tccaatcgaa ttatcacatc cacaaaatat acaaaattat
     1981 agagaaaaat caatacatag aatatcctat taaaattaaa tttctaaatt aacctaaaac
     2041 cttaaaatac aaatgttaga catttataca tgagatgggc aaataaactc gctgggtatt
     2101 tttttggagc actacttgtt ttatttggtt ttctttggat tgcatctaca atgacaatac
     2161 ctgtaatatt tataggatct atcggattga taatagtatt tggaggattt gccattattt
     2221 atttaggtca tagatccgga agacagaaaa aactactcca ataagagtaa ttcaaaataa
     2281 caattttctc ccaaacaaat attttgaatt ttacaatttt caacaacaaa tgcacacaat
     2341 caaattatca tattctataa atcatcttta aatcaaatca gtgcttatgc catttttaac
     2401 ctaaaaagaa ttttatttcc atttttaatt ggttattgat gaaatcaata agatcatctg
     2461 agatgagaat atattatata cttttccaga atcaacacct tatgtgatat agcaatatga
     2521 gcatagaata ttctatattc ttcattaccc tgatactagt tcgtctttgg gctgtataac
     2581 aatacctctt tccataaaac tatctgtaga atcgcggaac aactatatga tgtatgaatc
     2641 tataatgtaa atgatttttt tgaaataagg ttactttgat taatagctag tacaaagtct
     2701 ttgaatacct tgttaagatc tgtataattt agagttagtt ataccaatca atgtgcctta
     2761 aagaaataat acaattaatg aaataataga aataatacta ttatactaat tatcatttca
     2821 attaaaaaat gtaaaggacg aagaaatttt gataaatttt ttggtaatat aatgatgaca
     2881 tttagtcaag aaagtagttc aaatacatcc agttaccaaa actcattttt acaattaact
     2941 attctactat atactattaa acgtgataat gtgacttaaa tattcattca attttttagc
     3001 tattatttga ataggtctgt attaattatt gcattgttgt atttcctaaa agttgtgttg
     3061 tgttagattt gatgtaatta ataactagac aagatccgat ctttttatca ccctcggtag
     3121 aaccaaaatc cataatttta tcaaattcgt cacaaatgaa agtatccagt cccaaaccgc
     3181 cgatcatgat atctgatccc tctcctccat taatctcatc acttcctttg cctccatcaa
     3241 ggtgatcatc tccttttcca ccatctatat tatcatcacc ttcagcacct gttattacgt
     3301 caatatctct ttctccaatc aatttatcat tgccctcgtc gccttcaatt atgtcatttc
     3361 cttgttgtcc ttttattaag tcattacctt catctccatc tagttcatca tcgccttttc
     3421 cgccatttag ttggtcatca ccttcttttc catttaactc atcatttcca tcaccaccct
     3481 taaatttatc atcaataata gttccttcta tcttgagatc tactttgggt ttgacaatgg
     3541 aaggaggcga aagtgtatta ttggtgatat tattaggaga tgaagaatta tcgatttggc
     3601 tataggcaat ttgtatgatt atatattgca caggtaacat cagtaatatt attaacatca
     3661 acatacataa actaagacta ttattaccca attcctaatc atttctataa ctaatggaat
     3721 ttaaaatatg gtaggttaat tttattattg ttattaatga tttaatcttc ataaacaatt
     3781 atcttattga agttagacct actacaaatt tttgtaatag ttataccctt ttcttaatat
     3841 ctttagagtt atgagatatt taaagtgtta ataaatggaa tattagaata attttaattg
     3901 ataaagtaga atagaaatta caaaatattt tatctctcta tcatacctta aattattgta
     3961 ctaccgggac gaatttttcc tattgaattt aaaatgatca tctaatacaa atattatatg
     4021 taattttcaa agccgctgat taattacaat atgtggatat taagaaatag gaatttttat
     4081 taaattatac tcattattaa tgtgaaaaat tttcatttat agttagatat tattgttgac
     4141 aatttggaca ccaaaatgtt gcacgactag ttaaagtctt ttctgattta atcatagatt
     4201 tgcatatcca acattctttt cctgatcttc ggaaaaccca atgcctagta ttccaagaac
     4261 tattctcatt ttttaacaaa tgtctagttc taccagagtt cttatatcca tacctaagta
     4321 gttgtggaat tccttcaaaa agaatattct cttctgttgt ctcaagttgt gatatttgtt
     4381 taaaaggcca aattttacaa ataaataaaa tttcagattt atatttatta cctatacccg
     4441 agattatttt ttgatctaat aaagcctcag aaataagtag ttgttgtttt gtttgtaatc
     4501 tttttttagc ttcatctatg tcgaaaatat cgcctaagca atcgggacca agagatttta
     4561 ttggttcttt ttctgcagga ttatctattg aaaattgtaa aattggacca ttaaattcaa
     4621 ccaatactgt ttccttagta ataattgtta atctgacacg ggaatcctct cttacatctt
     4681 taacattttt tactaacact ggattttttt tccattttga ccgctcaggt ggtattgcta
     4741 atccattatc atacttgtgc cgatcataaa tcctccattt tccccacatc atcatatggt
     4801 tacgaagata aatacctgaa gaaaattgga ttacaatatt ctttccaaat gtcttaattt
     4861 tttttaattt tgatcctata attttatttt tgatatcgat attcaaattt ttaaatatga
     4921 tatcttctat ttttttttcg gatagtgaag atgatatttt atctgctaca atctttacct
     4981 caggaccttc tgacatatta actctaagtc ttaatttaaa atttatagat ttgtataatc
     5041 attaacagct atgatattac tttggtgaga tcgaaataat ttatgacaag aacaatctaa
     5101 taataacctt atttttagaa tgatatccgt gctatatact atatttgtat gatttattta
     5161 acaaaaagga atggtctgaa atattaagcc attaaatgaa tatttttaat attttcacaa
     5221 tttctatatt tattcaaaat attatttact gattcgataa cgatattctt aaatttttct
     5281 ggagcataag cctgaaaaga ttccaattct atctgaaata gtcttccata ttttctaatg
     5341 aaataatctt ttcctaaatt gctgtctaat tttcttatta tttcaggatt gggattttct
     5401 ggtaaatgaa attttctcat ttgattttct gtgactgcaa ttctattaaa ttcaatattt
     5461 aaattgaaat ccattagagt attattgatg attttatcaa tattgttgtt aaatagatca
     5521 aaattaccaa aatacagtat gtaaatcttt ttatcatctt tgaaagaaca ttttaaacgt
     5581 ttaatatttt tattgataaa ttccaaatta ttagttcgtt tatttgaaat tattttaaca
     5641 cctgaatttt tcaacaaact atggagggta aaactaggtg catttttttc gacccatatc
     5701 tctatatctg tgtatatgta attactttta ttattgaatt tataatcttg tattgaagga
     5761 tttgctgagg tttcttcaag gtaattgact attttttgaa tgtattcctt tgcattaaca
     5821 aattcaggat acgaatttgt tattctaggt aaatcttgtt caggaaagca accaataggt
     5881 aaatctccct tgatttttgc atgattaaca tatttactaa gatatttata atagttatat
     5941 gaatttggaa taatatttaa aaatactaga gaataaaaaa tagttttgaa cgttggaatt
     6001 ataccatatc tattaaacaa tgctaacctc tcttttatta ttggtattac atcattcttc
     6061 cagttaactc ttttttgtgt attgtgcata gttattaacg gttaataaac gtatataaac
     6121 gttaaattta ttaaggaatt tcacatgatt atattaaatg ccccaaccag gtcaaagaac
     6181 aataactttt agtggaaagg tgttaaaaaa attagaagag atatatatac tggagaaaaa
     6241 caaaaatcca tctctatcct ttgcctcttt tgtgtctgaa tccgctttga ttgaacttga
     6301 gaggaggcga atactaaagg aagcaccatt aatctcttta attagtttaa atgatgatat
     6361 aattattttg aaggatttaa gaaaaaataa tagattaatt gaagtacaaa ttagaaataa
     6421 aaacttgaaa tgtataactg atgataaatt tgattgtatt catgttggat ttactttggc
     6481 attgccagaa gttagaaaag ctttaagtaa ataaatagat aaaaaaattg ttatatttca
     6541 tactattata attataaaag aggtcatcaa atgggggtcc tattacaagg aactaaatct
     6601 aaatacaatg aaatattata tttttgataa aaatttaaac tctggatgaa ttctgaaaaa
     6661 cataaaaaaa aaacataata tgcaaaccat atagtatggt aacttgttct tatattata
//
LOCUS       DASVEV010000252         7253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_324397, whole genome shotgun sequence.
ACCESSION   DASVEV010000252 DASVEV010000000
VERSION     DASVEV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324397"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VF222_10990"
     CDS             complement(<1..509)
                     /locus_tag="VF222_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142992.1"
                     /translation="MKFIYSLLVYALPIVITLYIIPMSISFSRTSDTIEIKTLLTKGD
                     FMITSLNFQKTNLTFGEGNKICPDNNCSYEFEKTSLDDGFGAQNSKFLSGTLKIGDKG
                     DYEDNFPAYKFYKLSGLFNLVNRKENITTGENILIYQGDLLFDTGNEMFTPSKLEYQS
                     QIKLYDSQK"
     gene            complement(1768..2892)
                     /locus_tag="VF222_10995"
     CDS             complement(1768..2892)
                     /locus_tag="VF222_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7142993.1"
                     /translation="MWHHSNKGNKSVKFTDDSIKCCICFIEFESSINNIDIADDPSHV
                     REYYSTVINSLSQIINRNNGKIVKSLGDRLLCYFLNFSDLNNEKAVEEVIQCGFEILE
                     KREDINKELLNNNLPSVESYKISLDYGVVDLALAGDNYQIDIFGSTVNLCSKINSSSL
                     SIPNEIITGNNFYRIIKSFSNIQNNYNFINNGELKVTETIGYLTHNIRRKNTLIHNDI
                     LTINSSIEKQLHSLKEKPIENKNKKSFFSNKKTNSKKIILVDDEYDILFTYESFLKGY
                     DYDIISFTDPFVALNHIKDLPNFNDLLIIIDIRMKTLNGFQLHQQIKSIDPTIKILFV
                     TALDIVDELISIIPGISHNQIMKKPVEKKLFTQTVKKLLH"
     gene            3802..4023
                     /locus_tag="VF222_11000"
     CDS             3802..4023
                     /locus_tag="VF222_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142994.1"
                     /translation="MTKNEEAINAKVKIVIVILFTIITITVLHILINEGMAQEKGITP
                     IDPKNVTNQTLQDIPTEDTDMSTGIENKN"
     gene            4797..4982
                     /locus_tag="VF222_11005"
     CDS             4797..4982
                     /locus_tag="VF222_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142995.1"
                     /translation="MIELYSYELKNRNEIECYDRVMKPYPNYSTIWYNLGLGYKQKAK
                     EYFHRSKSLNKIRREMK"
     gene            6740..6958
                     /locus_tag="VF222_11010"
     CDS             6740..6958
                     /locus_tag="VF222_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7142996.1"
                     /translation="MIICNPEGRYEIANIIEKLDNVVNVDIINGPYDLVVEIIFDTMD
                     DLKEKIQHDISNIDRLMSTLTLIRSEDV"
BASE COUNT         2681 a          946 c          981 g         2645 t
ORIGIN      
        1 ttcttttgtg aatcatatag tttaatttga gattgatatt ctaattttga tggagtaaac
       61 atttcattac ctgtatcgaa tagtaaatca ccttgataaa ttaaaatatt ttctccagtt
      121 gtaatatttt ctttcctatt aaccagatta aacaagccag ataatttata gaatttatat
      181 gcaggaaaat tgtcttcata atctccttta tccccaattt ttaatgttcc tgatagaaat
      241 tttgaatttt gtgctccaaa tccatcatcc aatgaggttt tctcaaattc ataggagcaa
      301 ttattatctg gacaaatctt attcccctcc ccaaaagtaa gattcgtttt ctgaaaatta
      361 agacttgtga tcataaaatc accttttgtt aatagagtct tgatttcaat tgtatcagat
      421 gttcgagaaa aagaaatact cattggaatt atgtacaaag ttataacaat tggaagtgcg
      481 taaactaata atgaatatat aaatttcatc tgaagtttgt ctataattct gaccttttaa
      541 agatattgaa tagtccttcc cttctcatac tgtccattct ctttttcaat aatcactaga
      601 tatctctata acaattgtgc aagtgtaacc atcttggacc actgcatgat aagaaagatt
      661 ccctcatcat tggtttttga ttgattaact ttgttcacat atttttatat attttcaact
      721 cctataaaat tgctacacta gtcaacacag gcccttagaa tagccattgt ccaaaaccta
      781 acatatgctc gatccattct aggctatggc tattaagtaa actatctcca tttattgaat
      841 atcatattat caatttgaat actctcataa gcaagaaggc ttgtaattct ccatagcatt
      901 ctattatcga tttgctcaaa tgattgctat ggccttttaa aataagggaa taattagtat
      961 agtaaagttt ttaaccatta tatattcaga atggttatat tgataacata acattgttct
     1021 aatattttta cagtacctca tagaacgaga taatgagtta ctattaacgg taacaaaaaa
     1081 tagtgtataa ctaaatagga taaggacaac tctgcatctt caagcacctt tttttaaatc
     1141 tctatctatc ttttctcatt ccgataacta tattgttatt gtattctaag tgattatata
     1201 gtaatatata atttttccaa atctcaaata tgttacaaat ttcctaaata tattaataag
     1261 ttactaaatc ttggttgatc tttcattcta ttgataatgt cctcaaaata ttaaacatta
     1321 atttgtttcc aaatgcaata gctatctgcc tcctaccgtt aaagtaattc tcaattttaa
     1381 tattgatttc ttgaagttcc ctctctgata ttactaatga tactaatgaa atgttctttg
     1441 aagaaaatat cattgaaaat ataaagactt ttgaatatcg tattagacct aacaatagtt
     1501 aagcaataaa taatatactt caattacaaa aggaatatta caaggaattt aatgaaaaac
     1561 aatagaagaa taagaataac ggtttatcaa ttctcaaaaa tattatagta tctaaaacaa
     1621 atggagagga tatgagcaaa cccatatctg atatagaagg tacaaccata ttatgcatca
     1681 attagaaata ttagaatttg tattattgca atatatattg agataataat aaacgatgat
     1741 ctagatttaa tactgaagta ggttttgtta gtgtaataat tttttcacgg tttgtgtaaa
     1801 taattttttt tcgacaggtt ttttcataat ttgattgtgg gaaattcctg gaattatact
     1861 tattaattca tctactatat ctaaagctgt tacaaaaaga atcttaattg ttggatctat
     1921 agatttgatt tgttgatgta attgaaatcc attcaaagtt ttcattcgaa tatcaattat
     1981 tattaataaa tcattaaagt ttggaagatc tttaatatga ttaagggcaa caaaaggatc
     2041 tgtaaaagaa attatatcat aatcataacc ttttagaaaa gattcataag taaaaagtat
     2101 atcatattca tcatcaacta aaattatttt tttgctatta gtttttttgt tggaaaagaa
     2161 acttttttta tttttatttt ctataggttt ctctttaagg gaatgtaatt gtttctctat
     2221 actagagtta atagtaagta tatcattatg tatcaaagta ttctttcttc ttatattatg
     2281 tgtaagatat ccaatggttt ctgttacttt taattcacca ttatttataa aattataatt
     2341 attttgaata ttagaaaaag attttattat tctataaaaa ttatttccag taattatttc
     2401 attaggaata gacaaggagg atgaatttat ctttgaacaa agatttactg ttgaaccaaa
     2461 tatatctatt tgataattat ctccagctaa ggctaaatct actacaccat aatccaaact
     2521 aatcttgtag ctctctacag atggtaaatt attattcaat aattctttat ttatatcctc
     2581 ccgcttttct aaaatctcaa atccacattg aattacctct tcaactgctt tttcattatt
     2641 aagatctgaa aagtttaaga aataacacaa taaacgatca ccaagacttt ttacaatctt
     2701 accgttatta cggttaatta tttgcgataa tgagtttata acagtagaat agtattctct
     2761 tacatgtgaa ggatcatctg ctatatcaat attgtttata gaactttcaa attctataaa
     2821 gcatatgcaa cattttattg aatcatcagt aaatttgacg cttttattgc ctttattgct
     2881 atgatgccac atgagattcc tctcccattt gttttttcta tatataaaaa ctgttttgtc
     2941 taatagatag aaacgcatag aaatcaattt attattataa tttttggaaa ccctcggtgt
     3001 aatattctaa taaaatataa ttaatttttt agctattatt aaatataatt acaaatacat
     3061 aagttctata taccataatt aaattgtata ggtatcaata agaattgact aggttaatga
     3121 taagaagaaa tttttcatta gaatatatta tttgttagat taattgtttt attgaattaa
     3181 aaaaataata taagaaatct aataataacg agctagcgat attttctaaa gcgtaatata
     3241 acttttcggc gccgaaaagt tttagtatat attccttaga aagcatttga ccataggata
     3301 actacaaaca ccatcaaagt aaattcacta taattttaat aaatattgag aataatatcc
     3361 atatgtatag attatgacaa taataatatt ccagaaaagc aaagccattc atcactataa
     3421 cttatgtata attcgaaaat aatgaattgt caataaatca atcatgtata ggatatacca
     3481 tatttggtga taaacctgca agtcttttag gtgtattttt tactggtttc tcactgctaa
     3541 accactactc tataaagatt tgagtaaaat ttcaagataa atcaataaat agaaaatcaa
     3601 actaataata ttataagcat taaaaggtta aaaaagaata aataatattt ctattatttt
     3661 ctaataatat tagaacttaa tgtagatatt tggaaaattt aatattagct taatgaatat
     3721 cctttcaaaa attgaactat atgcgtataa attgcttcta agaaggtttg atatagtatg
     3781 ttatttagtt gatgaatgaa gatgacaaag aatgaagagg caattaacgc taaagtcaaa
     3841 atagtaatag taatattatt tacaataatc actataactg tattacatat tttgataaat
     3901 gagggtatgg ctcaagaaaa aggaattaca ccaattgatc caaaaaatgt aactaatcaa
     3961 acattacagg atattccaac tgaagatacg gatatgtcaa ccggtattga aaacaaaaac
     4021 tagattataa aatggtaagg ctttactaaa aagaattatc atttagattg ccacaattat
     4081 tgtttaagac ggaagttgaa atagttagtt aatactatat aataataaga atataataaa
     4141 taaatttaat agctacaact ttgtaaaata acaacacaat ttgaggaatt ggcctcatta
     4201 aggtatggat cattggatta taaagataaa acatctcact ttgttactat gataaaataa
     4261 gataggacta aaatgtaaga agctaaaaaa tacaaaagat tattggacta cgttaatgtt
     4321 aaaacctagt tggattttct tgctgttgct agtattacat ctaattatgt ctattaactg
     4381 attgttatac tgcttttata caattcgtgt ctgtttttgt gaaagccttt agattatcgc
     4441 gtgtttgttc aagtggtgta ttgtatactc tcaaatttga tgctagagca ttgtttatta
     4501 gaggttagta tttagcaggt aacttgtaaa gttatttacc attactgcgt gattttcagc
     4561 aattctatgt ggatggtgaa ttcacatctg gaaagataaa agttgtagca ttggactaac
     4621 cgcttataaa ggattcactc acaattcgtt tagaatagtc aaccattaat attcgtaatt
     4681 aaatactaca aaaatactct aaaaatatag cttgtacaat agccaaattt tgcaaactgt
     4741 gtaattgtaa gaattaatga agttatgctc aagaaaaaat tgaaagtgtt gtttgaatga
     4801 ttgaactata ttcatatgaa ttaaagaata ggaatgaaat agaatgctat gatagagtaa
     4861 tgaaaccata tcctaattat tccaccatat ggtataatct aggtttagga tataaacaaa
     4921 aggctaaaga atattttcat agaagtaaat ccctcaataa aatccgaaga gagatgaaat
     4981 aaaaaagatg ttatttcaga tttatacaaa taaactctat gttatctaat attgagtaca
     5041 attgaaaaat caaataaaca tttaataaaa attacatcac gaatttgtct ttgtctatat
     5101 tgccttttca gttccgatat attatactcc gacttcttga accaccaagt ttggcataac
     5161 ctaaacctct tgcttctttt tttgataatg tcttctggat tactatacta tagaataact
     5221 ggacaagata tttggataat attacatttt agattactaa atctattttt attattgcta
     5281 aagaatatca actcttgcgt tattaattaa tgtaatcaac tagttactat taacagttat
     5341 tacaggccat agcctatttt ggagcaaata acagattagg ttatggctag attgacgact
     5401 ctttatactg gatgatatca atatcttttg aaaattatat ttcataattg aagaaaagtt
     5461 agtaatagcc atagcctact atatggagca agcagtagat accggctatg actaaatctg
     5521 gttgttgttt agagtagtct attacacgct ccagaatatt taataatagg tgaacaaagt
     5581 tcacccaagg atcaatgagt aatgaaccat tccagttttt atttaacaaa tttcagaata
     5641 tactacaata gttcagtgtt ctattgttac ccaatgggtg ttatgcgaca tatctaacac
     5701 ccattttcaa aacaactata tttgagatta aagctgaagt tttagctctg tccttttcct
     5761 ttaaatagat ttagatatta agagaaagat tatagctata aaaagtatga ggttctgggc
     5821 tctagttatt ttccttatct cattattttt gcttctgtaa ttggatatta tattggaata
     5881 actcaagtaa gagattttta gagattggca aattcttatt ccaatttcta cactaattag
     5941 gttggcaata ggtatgtgga gacgtaatga aatggtttaa tgaaccatca ttaaaatttg
     6001 gtaaaaaaac ttctgatcac tgtatttatt ttattgatgt gattaagaat aggggaaaag
     6061 gaaaagctga agaatgtgag aggtaggatt agtatcggaa atacatcggt ttcaatatca
     6121 aaattaataa tttaaaatct acgtaaaagg atagtttttt gttttgaaaa atgggtgtta
     6181 gatttgtcgc ataataccca ttcttgacta cttcacccaa taaatagagg ttggtacata
     6241 taaatgcaca ataaagaata aaaatattat agattttttc aaaagcaaac gccgaaaact
     6301 gttatttaat gaatgattcc atagaaaaaa cagctattta ttaatgaaaa tattctattt
     6361 agatattgtt tgaggaggaa agcgaaagga taggaatgta aattcaattg gaaaaaagag
     6421 ataaaggttt tatactagga ttagccggtc ttgtagttgc aggagtcaga ttagttctat
     6481 cgtatttgga atacactaat cagtataact gttagcggta ctcaactatc ccatcttttt
     6541 ttccaagcat tataataaaa actaagctaa atttgtttct cgaaaagtat ccgacgattt
     6601 ttgacagttt gttgtatctg acaaggactt taccgaacac tatttacaaa cttttttata
     6661 atcctatcat gatttaacaa aatgcactgt tccttttata gatctgttat ttgtatatga
     6721 catgttaaga gcatatctct tgatcatttg taatcccgaa ggtaggtatg agattgctaa
     6781 tattattgaa aaattagaca atgttgtaaa tgttgatatt attaatggac catacgacct
     6841 agtagtagaa ataatatttg acacaatgga tgacttaaag gaaaaaattc aacatgacat
     6901 tagtaatata gatagattaa tgtcaacttt gactttaatt cgttctgaag atgtttgaaa
     6961 tattaaaata ttaaatgaat aatacaacaa aattaaatga tgtactatat tgtttattca
     7021 tcgtaaatgg atattaatcc ttaatattga atccaattca acttcctaaa aagaaatatt
     7081 caaataagaa tattcaattt ccgtagtaga acatcacata atacaaatct acaactattt
     7141 ttatcataat aatctttgtt tacgatagta gttttaatta caaacatatt actttattat
     7201 caatttagaa tataagtttc tgttaacttt gttaattgat ctaaatttta tgt
//
LOCUS       DASVEV010000253         8790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1489317, whole genome shotgun sequence.
ACCESSION   DASVEV010000253 DASVEV010000000
VERSION     DASVEV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489317"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..915
                     /locus_tag="VF222_11015"
     CDS             436..915
                     /locus_tag="VF222_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142997.1"
                     /translation="MKILVISSLLILLLLSSTLLLPSLTPVKGQESITVNCSFENAKL
                     TKIGHSLEQDREEMRENYKRYGDSALNALNSLPEESAKIIKTNLETEILMEKVKTNPS
                     IEYANCSHINAVEIELITTLKQGEEKWNEYWNNQEADFGKNEIKQMKEYIKLHIHNE"
     gene            complement(1054..1584)
                     /locus_tag="VF222_11020"
     CDS             complement(1054..1584)
                     /locus_tag="VF222_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142998.1"
                     /translation="MHWEIEVIIRVEDVEIDYINEGLLLHHSITDIENPSSIWIKERK
                     ENGKIVEENLLDNQYNYRIEFKAIVFSTEEQESLRRYINRMNGVDSKKMLFKTLTGKE
                     KASKAISVASISAIIVFIIIFSHEYIVSLAHNGHIELSHFSTIIAFSLAGAGATFFID
                     YLIVWKEKFGGLNFNK"
     gene            complement(1628..1888)
                     /locus_tag="VF222_11025"
     CDS             complement(1628..1888)
                     /locus_tag="VF222_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7142999.1"
                     /translation="MSSTSEEKLHGTIMIECRGKSRAMIMKNVTNIPNVVRVSKTEDD
                     TNGGILVTVHGSKDDIKKVKNQIWELDNNKNIKINSINYSYS"
     gene            2548..2925
                     /locus_tag="VF222_11030"
                     /pseudo
     CDS             2548..2925
                     /locus_tag="VF222_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            3154..3939
                     /locus_tag="VF222_11035"
     CDS             3154..3939
                     /locus_tag="VF222_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEX7143000.1"
                     /translation="MLKATVFPNDVARNLADPNAPTVGTRVEVVHINSFQRGVENPQY
                     EDEDDEMWFHWYTFFPSITELPVPNRFHIWTQFHQNLTESNCPVINGQGSVKCGAVPI
                     LFNLRDYGSGPLLQFLVIDKSNGNFWESLWNASLQRGGWYEFLFHVKWDKCGDTNVKV
                     YDRNNYNLDGTCKNNNGGFVELWVKQPSDNALVKVVSKTDPDAQRYTMDDDGLVYAKQ
                     GLYTGTGGTTSQTIYHDGMEIAKCPSTHPNYHPNTGKCFTTEP"
     gene            4276..4668
                     /locus_tag="VF222_11040"
     CDS             4276..4668
                     /locus_tag="VF222_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7143001.1"
                     /translation="MIDSIGGIVIFVSNQAMAIEFYTQKLGFDIKGEYPYKNTKWVEV
                     APKNSTTTISLMEPHSDMMTNEEIAQAKKEIGTMTNFWFYTKNIDDTYKELQEKGVNI
                     TEPKKQDWGGIMSQIKDQDHNILTLISS"
     gene            complement(4903..5805)
                     /locus_tag="VF222_11045"
     CDS             complement(4903..5805)
                     /locus_tag="VF222_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7143002.1"
                     /translation="MEYYLGCSGWSYDGWKGPFYPQDLDNRYWLSYYSKIFDFVEIDS
                     TFYRIPSKFMVNNWNNRTPDNFRFAVKFPKVITHDKRLKDVGKDIEQFYDAMESLYDK
                     ILVFLIQLPPSLQIAEGLDLIKNLQYQLDPSFRYAIEVRHHSWFNELFYNVLKERNYS
                     IVWSQQDILITPPILTTDFVYLRLIGDRSINERDFGKINKDRTKEMEIWSKILADVDR
                     KVNGIKMAIIAANNHYAGFGPMTAKLFADMMNLKDRIRSFPILDYKLPPNEIFRSENK
                     QNYRTYKQQQYSKPRQTDISEFFK"
     gene            complement(5974..6519)
                     /locus_tag="VF222_11050"
     CDS             complement(5974..6519)
                     /locus_tag="VF222_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143003.1"
                     /translation="MGRTIPSFRIATVLEEEKWKLFRKYLKNKKDKKLFNEMFSVARL
                     YNSACSNAVNPIRIFPILMSIIFHHYKKILSKSVIGFDNNSLSSARYYDINDYNNNNS
                     TTILKQELDKWYNFSFVLRKQNRELFDRMIQLSSYKYSLSINAKGKEFSTESLLISLI
                     FDQHKMMLPNSFFNNKIDCYL"
     gene            complement(6664..6840)
                     /locus_tag="VF222_11055"
     CDS             complement(6664..6840)
                     /locus_tag="VF222_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143004.1"
                     /translation="MSTTETIGCPFCSKANEVTSPDPEYKKASNTDKHGAKSAKYVCN
                     SCNQENVVYWTKEK"
     gene            6989..7186
                     /locus_tag="VF222_11060"
     CDS             6989..7186
                     /locus_tag="VF222_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143005.1"
                     /translation="MTSEKKVDLVNSPEVQVMKEKEKDFIDIDFKRLEEQKFYRVEYE
                     GDVYAVEKLADGKIAFYEVVE"
     gene            7189..7395
                     /locus_tag="VF222_11065"
     CDS             7189..7395
                     /locus_tag="VF222_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143006.1"
                     /translation="MANSESKPVDDLSNRSLKKIGEIDPKLFESNNEKEIEVRSWKKQ
                     KSETWHIYLDTNTGHLRKWKSSSS"
     gene            complement(7504..7929)
                     /locus_tag="VF222_11070"
     CDS             complement(7504..7929)
                     /locus_tag="VF222_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143007.1"
                     /translation="MSGNNSDNEKIDTTKFLYAFTQSVLNSDRSIRWVGITDQNGIII
                     NERDREGLKPFLTIEENHEFAINTINRHKTRLKFEPKMGDLTYTFRRYTRMNRCLIPI
                     NNKNKNYYLILTIDFDQYDFDKIITEKIIPLIKQEKEKF"
     gene            complement(8011..8175)
                     /locus_tag="VF222_11075"
     CDS             complement(8011..8175)
                     /locus_tag="VF222_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143008.1"
                     /translation="MAATIYDGTCKNCGSDLSVWIEVRRNVDGIPKPEVWCIDCIRGS
                     NPNNNNNKQN"
     gene            8596..>8790
                     /locus_tag="VF222_11080"
     CDS             8596..>8790
                     /locus_tag="VF222_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143009.1"
                     /translation="MSTDSPIYNPKDVNKKEARGLRDEVDLGEVQEVGTEYVLTKKGT
                     IDSDKFYIPKNLVERFDGRTV"
BASE COUNT         3192 a         1252 c         1253 g         3093 t
ORIGIN      
        1 gaacattcag agttacagga caaaatatgc gaaatgatta caataaagca atttgcaaat
       61 atctctccag gttttgatgt tcaacataga cctttcttca gagattgaat atctatagtc
      121 cttcaatttt agtttgtaac tgtttcttta gtatcaattg tataaacccg tgaatgcaag
      181 taatggggtc gaataccagt tcctctatac aaatcatatt ttaaaatagt aaagtaatat
      241 tgcattaaga ttctttaatt aatattttat aacaattata tcgataatga aatttatttt
      301 aatggtttag ttaatataaa taaataataa aaaatattat tctgtagtaa ttactaataa
      361 aatattgtag aaattttatt cttttaattt tttaaaaaat ttatttaata taatttttat
      421 agattgcttc actatatgaa aattttagtg atatcaagtt tactaatact attattactt
      481 tcatctacat tattgctacc ttctttaact ccagttaaag gacaagaaag cattactgtc
      541 aattgttcat ttgaaaatgc taaattgact aaaataggtc attctcttga acaagataga
      601 gaagaaatga gagagaatta taaacgatat ggtgattctg cattaaatgc tttaaactca
      661 ttacctgaag aatcggcaaa aataattaaa acaaatttag aaacagaaat tcttatggaa
      721 aaagtaaaaa caaatccaag tatagaatat gcaaattgtt ctcatattaa tgctgttgaa
      781 atagaattaa ttactacatt aaagcaaggt gaagaaaaat ggaatgaata ttggaataat
      841 caagaagcag atttcggtaa aaatgaaatc aaacagatga aagaatatat caaattacat
      901 attcataatg aataagatga aaaaatatta attatgatat tacaaaaata atttacagat
      961 tattggtttt agttaatata attagtaaaa tgacgatatt ttgccacttt cattactatt
     1021 atcactacct attttgaagt ttaagatcct tattcattta ttaaaattca aaccaccaaa
     1081 cttttctttc catactatta aatagtctat aaaaaatgtt gctcctgcac ctgccagtga
     1141 aaaggctata atcgtagaaa aatgactcaa ttcaatatga ccattatgag caagtgaaac
     1201 tatgtattca tgactgaata tgattataaa tacaatgatt gcagaaatgg atgctacaga
     1261 tattgcctta ctcgcctttt cttttccagt gagtgtttta aataacatct ttttagaatc
     1321 tactccattc attcgattga tataccgtct gaggctttct tgttcttctg tagaaaaaac
     1381 tatcgcttta aattcaatac ggtagttata ctgattgtcc agcagatttt cctcgacaat
     1441 ctttccattt tcttttcttt ctttaatcca tatgcttgac ggattttcaa tatctgttat
     1501 gctgtgatga agtaataatc cttcatttat gtagtctatt tctacatctt caactcgtat
     1561 aattacctct atctcccaat gcattaatga atattaagaa attttaaaat ataataaagt
     1621 taatactcta actataacta taattaattg aatttatttt tatattttta ttattatcaa
     1681 gttcccaaat ttggttttta acttttttga tatcgtcttt agatccatga acagtaacta
     1741 aaatcccgcc atttgtatca tcttctgtct ttgatacacg aacaacatta ggtatattag
     1801 taacattttt cattatcatg gcacgactct ttcctctaca ttctatcatt attgtaccat
     1861 gtaacttctc ctcacttgta ctgctcatat aattatatgg acattctttg tatatgtata
     1921 tttctcatag attatttaaa attacaagac atttttaatc attcacagtc tagtcataat
     1981 atggacatta taacatattt ttgtctgtat cagtagtaaa ttattatatg gtaaaattac
     2041 aaggtattta cttacaaata taaaacaaaa actggagaaa ccaaaaatca ctataaatat
     2101 attcctaata tcatgataca ttttattata ctgtctaaaa cgtagagagg agtcatattc
     2161 tgaatttcat ctagaataat cctctgctaa ttcgcctatt ggtgcattta tggtatttac
     2221 tgtattatta acgaacagtt taccaataaa ttagcaattg aatattgaaa atattattaa
     2281 tgtaatcatt agttccaaaa atgggattaa ttgactacat gaaggtattt aattaaattc
     2341 tactaataac ctatcatcct attcaatatt aattttatac aaatcgtaga tataattcaa
     2401 tattaatttt atacaaatcg tagatataat atttttattt taaaatttta cgtttactat
     2461 ttaaaggaaa agttgagtta cattgtgatt aatattgaaa actagatctc acagcaatat
     2521 tagtatgctt ttaattgcat tagttttttg acttctgctt caattgtaat aggcaatact
     2581 aataatgtat ctgtttatgg agaacaatat ggttataatg ataactatcc tactaaagaa
     2641 ataaacaaat atgaatgtca gactggtcct ttcgaaggct tctttacaag ttcccctgaa
     2701 ttctgtaaat caaaagttac acccgatcgc gatagagatg gtggttccac tcctaaccct
     2761 gacacagatg gcgatggcat aaatgacaat gatgacaatt gtcctactac accaaattca
     2821 gaccaagaag actctcctga taaggatctt ataggtaatg tatgtgatcc agatgatgat
     2881 aacgatggta ttaatgacaa tgttgacaac tgtcctctta aagcaaatac aaatcaaaca
     2941 gatactgata aggatcttat aggcgatgta tgtgatccaa ctcctaataa tattggagta
     3001 gaacctatat tcttctcatc tctaggctat gaggaaggaa acgatattag gggttgggag
     3061 tggggaaaat ccgaggctgc aggtccggat aggttagata cagtcaataa aaatagcata
     3121 tccaatactc taggagaccc aaatggaact caagtgctaa aggctactgt atttcctaat
     3181 gatgttgcta ggaatttggc ggatcctaat gctcctacag ttggcaccag agtcgaggta
     3241 gtacatatta attcatttca acgaggtgta gaaaatccac agtatgaaga tgaggatgat
     3301 gaaatgtggt ttcattggta tacattcttt cctagcataa cagaattacc agttccaaat
     3361 agatttcata tttggacaca attccatcaa aatctgacgg aaagtaattg tcccgtgata
     3421 aacggacaag gttcagtaaa atgtggtgca gtacccattt tgttcaattt gagagattat
     3481 ggttcaggac cattattaca atttctcgta atagataaga gtaatggtaa tttttgggag
     3541 tctttgtgga atgcttcatt acaacgtgga ggatggtatg aattcttatt tcatgtaaag
     3601 tgggataaat gtggagatac taatgtaaag gtctatgatc gaaataatta caatctagat
     3661 ggaacctgta aaaataataa tggtggattt gttgaattat gggtaaagca accctccgac
     3721 aatgcattag tgaaggttgt tagcaagaca gatccagatg cacaacgtta tactatggat
     3781 gatgatgggt tagtttatgc aaaacaagga ttgtataccg gtacaggagg tacaacaagt
     3841 cagacaattt accatgatgg tatggaaatt gcgaaatgtc cttcaactca tcctaattac
     3901 catcctaata ctggcaaatg ctttacgaca gaaccataac ataaacctca tcttttttta
     3961 caataatcta agtcaaaatg taaactataa ccactaatat agaaacaata aattttaaag
     4021 taagaacccc caagtgaacc ttatgggttc gaatcccact ccccgcgtcc tactcgtgga
     4081 gtcatgtcta ttacaaatat aaattgattt tttcttaaac ttaataataa ttaatcgaaa
     4141 tatttatcta atacatgtag atcgagttgt aaattagttt tagaatattc ttctaaacga
     4201 ttaattttct ttccagtctt ctccacatta catatataga taaagattat aatcatgatt
     4261 gattttcata tttatatgat agatagtatt ggaggaatag taatatttgt ttcaaatcaa
     4321 gcaatggcaa tagaatttta tacacaaaaa cttggatttg atattaaagg tgaatatcca
     4381 tataagaata caaaatgggt tgaagtagca cctaaaaatt ctacaactac aataagtttg
     4441 atggaaccac attctgatat gatgacaaat gaagaaattg cacaagcaaa aaaagagata
     4501 ggaactatga caaacttctg gttttatact aaaaatattg atgatacata taaagaattg
     4561 caagaaaaag gagtaaatat cacagaacct aaaaaacaag attggggcgg aataatgtct
     4621 caaattaaag atcaagatca taatattttg acattaattt cttcataata gaatcatgct
     4681 cataacaaat aataattatt tctctataat ggaccttaat agaattctaa aatagaaata
     4741 ttgaattatc ttcaatttgt ttgaatatat tttaatatta gaaatagcac tcacatttga
     4801 tggacgaagg ttagaatccc actaccagca ctacggatgg gatgtaatta taaaaagaat
     4861 aatcagatag aattattatt gattgaatca ttaacaatta agttatttaa agaattctga
     4921 aatgtcagtc tgtcttggct ttgaatattg ttgttgttta tatgttctat aattttgttt
     4981 attttcgctc ctaaatattt cattaggagg taatttgtaa tctaaaatag gaaatgacct
     5041 tatacgatct ttcaaattca tcatatctgc aaataacttt gcagtcattg gaccaaaacc
     5101 agcgtagtga ttatttgcag cgattattgc catctttata ccatttacct ttctatcaac
     5161 atctgctaaa atcttagacc atatttccat ttctttagtt ctatctttat ttattttacc
     5221 aaagtctctt tcattgatgc tcctatctcc tattaatctc aaatatacaa aatctgtagt
     5281 caaaattgga ggtgtaatca aaatatcttg ctgactccat acaatactat agtttctttc
     5341 tttaagaaca ttgtaaaaaa gttcattaaa ccaactgtga tgtcttacct ctatagcata
     5401 tctaaaagat gggtctaatt gatattgtaa attctttatc aaatctaatc cttctgcaat
     5461 ctgtaaagat ggaggtaatt gtatcaaaaa aactagtatt ttgtcatata aagattccat
     5521 cgcatcataa aattgttcta tatcttttcc tacatctttt agacgtttat catgagttat
     5581 aacttttgga aactttacag caaatctaaa attatctggt gttctattat tccagttgtt
     5641 aaccatgaat ttagaaggta ttctataaaa agtagaatct atttcaacaa aatcaaaaat
     5701 tttagaataa tatgacagcc aatatctatt atctagatct tgaggataga atggtccttt
     5761 ccatccatca taactccatc cagaacaacc tagataatat tccaatacat tattttacca
     5821 ctggaaagca aaaactattt ttttaaaaag tattagtctt ttgttctata tttcctaata
     5881 aagcagacaa agagggagta gaataccgta atttgaggga agatacagaa tatcaaaaga
     5941 aatattcttt atcaaattag tattgatatt cattcacaaa taacaatcta ttttattgtt
     6001 aaaaaaagaa tttggtaaca tcattttgtg ttgatcaaat attaatgata ttagtagtga
     6061 ttctgtggaa aattcttttc ctttagcatt tattgataac gaatatttat atgatgataa
     6121 ttgtatcatc ctatcaaata gttctctatt ttgttttctc aatacaaaag agaaattata
     6181 ccatttgtct aattcctgtt ttagtattgt tgtagaatta ttattattat aatcgttaat
     6241 atcataatat cttgcagaag acaaagagtt attatcaaag ccaatgacag actttgataa
     6301 aatctttttg taatgatgga agattattga catcaagata ggaaatattc gaattgggtt
     6361 aacagcatta gaacaagcag agttatacaa tcttgcaact gagaacattt cattaaataa
     6421 tttcttgtcc ttcttattct tcaaatattt tctaaataat ttccattttt cctcttctaa
     6481 aactgtagca atcctaaatg atggtattgt ccgacccatt attattattg ttgaaagtta
     6541 tatgcagaat ataatgagta gaacactaag acgagagaga gggtctatag aaaacgttct
     6601 agaacaaaag tatcgtatcc aagaatctaa tatttagaag aaccattatt ttaaaagtta
     6661 tgattatttt tcttttgtcc agtaaactac attttcttga ttacaactat tgcaaacata
     6721 tttcgctgat ttagcaccat gtttatctgt gttagatgct tttttatatt caggatcagg
     6781 agaagttact tcattagcct tactacaaaa tgggcatcca atagtttcag ttgttgacat
     6841 aactcatttt tactatatcc tctttattaa gatattatgt ttgttaataa ttttatcaat
     6901 ctataatagt tgctgaaaat atgaatattg gaacagtatg ttagagatga atcaaatagt
     6961 tattaattat atttaacata ctataacatt gacctctgag aaaaaagtag atcttgtaaa
     7021 ttcacctgaa gttcaagtaa tgaaagaaaa agagaaggac ttcattgata ttgattttaa
     7081 acgtcttgaa gaacaaaaat tctatcgagt agaatatgaa ggagatgttt atgctgtaga
     7141 gaaattggca gacggcaaaa tagcctttta tgaggtggta gagtagaaat ggctaattcc
     7201 gagtcaaaac cagtagatga cctttcgaat agatctttaa aaaagatagg agaaatagat
     7261 cctaaattat tcgagtcaaa caatgaaaag gaaatagaag taagaagttg gaaaaaacaa
     7321 aagtcagaaa catggcatat ctatcttgat accaataccg gtcatttgag aaaatggaag
     7381 agttcttcaa gttagactat tttttataga ctaatatctt atattagtag catcagttac
     7441 aatgtagttt tcaatgataa taaatacaag aaacaaaaat aggatataag atttaactct
     7501 cgtttagaat ttctcttttt cttgtttaat taatggaatg attttttcag ttattatttt
     7561 atcaaaatcg tattgatcaa aatcgatagt taaaatcaaa taataatttt tatttttatt
     7621 attgattgga atcaaacatc tattcattct tgtatacctt ctaaacgtat aagttaaatc
     7681 acccattttt ggttcaaatt ttagtcttgt tttatgtcta tttattgtat taattgcaaa
     7741 ttcgtgattt tcttctatag taagaaaggg ttttagtcct tctctatctc gttcatttat
     7801 tataatacca ttttgatcag ttattcctac ccatcttata gacctgtcag aatttaatac
     7861 actttgggta aaagcataaa ggaatttcgt agtatctatc ttctcattat cactattatt
     7921 tccagacata tttttttcct attagtaaag taatttcgat tataataaaa aagttatttt
     7981 aatgtaaaac tgatctcata ataataaaat ttaattctgc ttattattat tattattcgg
     8041 attgctacca cgtatacaat ctatacacca aacttcaggc tttggtattc catcaacatt
     8101 tctccttact tctatccaaa cagatagatc cgaaccacaa tttttacatg ttccatcgta
     8161 gattgttgcc gccattttca ttgatatcta caatctcata taaattgttt aaaagaaaga
     8221 aaaattattt aactttatta agtatagata aacaaaacta gaacaatcat taacatgaga
     8281 ggaacaatgt tctagaagaa aagtgtgcaa cgttgcacac taatgtcaat aaatttgggt
     8341 gtctcaagat tagtacgctc agaaaccgaa tcagaattga aattcttata cctttaaatt
     8401 ataataatgg aaattaaaaa acttcttgaa caagaggata tttatattaa actagccata
     8461 gtttcatgta ctgttatttg ctcatactca tggatattga gtatggcagg gctttttcaa
     8521 ttaataagta tttaacaatt aaatcaaatt aattgaaata ggttatttat atgaaatgta
     8581 tgcaattatt aatagatgtc aactgattct ccaatatata atccaaaaga tgtaaataaa
     8641 aaagaagcga gaggattaag agatgaagtt gatctggggg aagttcaaga agttggaaca
     8701 gaatacgtcc taactaaaaa aggaactata gacagtgata aattctatat tcctaaaaat
     8761 ctagtggaac gctttgatgg tcggacggtc
//
LOCUS       DASVEV010000254        21569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1686456, whole genome shotgun sequence.
ACCESSION   DASVEV010000254 DASVEV010000000
VERSION     DASVEV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 21569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21569
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686456"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VF222_11085"
     CDS             <1..867
                     /locus_tag="VF222_11085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7143010.1"
                     /translation="QIKHSYVDKILSGDCKEIISKIPNGSIQLTITSPPYRNAIDYSA
                     HTSKNGSYYRGKSNINTKEYLEEMIDIFNNKIFNATKEGGYCCIVIANEVVNGTLLPL
                     PHMVLSQLVEPFGKWNLHEEIVWHKVTGGTNRYGSFVINPYPKYYRANIMHELILVLR
                     KGNVSSGRTERTETLPATHEEWTKEIANSVWHIAPIPPGYIEHPCPFPEEIPYRLMKL
                     YSYEGDTILDPFNGSGQTTKVAFHLQRRFIGVDIINEYVKLARSRLIEPIHIRAEALI
                     ANWKKIPSHHNL"
     gene            1015..1716
                     /gene="aroD"
                     /locus_tag="VF222_11090"
     CDS             1015..1716
                     /gene="aroD"
                     /locus_tag="VF222_11090"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX7143011.1"
                     /translation="MAGRICVSIGAKTIKELTSSINKALDLSDFLEIRFDFLDKSEIL
                     GSMKIIESIKSRSVFTLRSREQQGKFNGNEFERLKILKMLYDANPMLLDVEYDTITAD
                     QSLNNYLKDKSNVLISWHNFSNTPSDEYLEKKIYDMKRFSNNLKLVTMATNITDSLRL
                     LNLYEKFQDSNLITFAMGDCGIMSRVLCTFYGRPPFTYASLEKSIAPGQLSVIDMRRI
                     YERINKHFLDNVITN"
     gene            1700..2545
                     /gene="aroE"
                     /locus_tag="VF222_11095"
     CDS             1700..2545
                     /gene="aroE"
                     /locus_tag="VF222_11095"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7143012.1"
                     /translation="MSSPIKTYCIIGDPVQHSLSPLMQNSAFSALGIKSSYISFRVPS
                     GDLKTSIESLKSIGIAGFNVTIPHKISILPYLDELDITASKSNAVNTVKNDGGKLIGY
                     NTDLYGFIQPLYKRNIDFKGIKILIIGAGGAAHAIVTALSYEKEIAEIIVVNRSSHNA
                     SKLVQHGLKLGLNTHKEDFDTLSKLALQSDLIVNATSIGLNNESSPIERDYISPNCIV
                     YDIVYKPIVTDLLGKAKEVHARVVYGYEMLIAQGAQAFKIWTGFDAPIIAMKKALFGI
                     FGEPT"
     gene            2542..3396
                     /locus_tag="VF222_11100"
     CDS             2542..3396
                     /locus_tag="VF222_11100"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR01920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7143013.1"
                     /translation="MTSISTKATVHGAISIVNAIATGKGSALGISLETTAEIHMRKGN
                     GKIIHHNRNSELFRYIIHNSIPKKILQDNDIFLTICSDIPIGFGLKSSSAVSSAVSLA
                     CYGVVKDTINDFKVLDTAVDASRKAKITITGAYDDSTACYFGGFVITDNYSNKLLRRE
                     NGPIDLLSLVLLPNNSVRKSHLKLKILSEFFEQALLSAEKSDFWKAMTLNGLLVSSLM
                     GYDYTPILLSIENGALSASISGNGPSIAVIIEKKNLTTIRSILENYGKTLVCKINNTK
                     ASIEKKID"
     gene            3389..4690
                     /gene="aroA"
                     /locus_tag="VF222_11105"
     CDS             3389..4690
                     /gene="aroA"
                     /locus_tag="VF222_11105"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7143014.1"
                     /translation="MTKLEISKSFLEGNIKCPPSKSYTHRAIAIASLADGKSTISNPL
                     LSRDTIATISGCKMFGIIIGKSNENVNCIQILGKNQFETPKDIIDAKNSGTTIRILTS
                     MAGLVKNGYTILTGDESLKTRPMQPLISALNQLGVHAFSSNEKNTPPLIVKGGGIKGG
                     LVSIEGGISSQFISSLLISCSYALTDVEIRVKGKQVSIPYITSTIETMKKFGISIEHS
                     KEYLNYYISNYKYRPTDFTIPGDFSSASLIIAAGVLCGGKIAIGGMNFHYPQGDMAII
                     DIVRQMGGDIKVNKEKGEVIIYGSNSLDGIECNLVNCPDLLPVVSILSLKARTPMRIF
                     GISHARFKETDRVSIITNELRKMGVDVSEKEDEVLINPVKGLKNVEFDSHNDHRLFMS
                     FTIASMLTNKSIVKGAESIDVSYPAFINEIKRLGANITLPK"
     gene            4780..5883
                     /gene="aroC"
                     /locus_tag="VF222_11110"
     CDS             4780..5883
                     /gene="aroC"
                     /locus_tag="VF222_11110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7143015.1"
                     /translation="MGGNSLGERFIVTSFGESHGRCVGSILDGCPAGLPLKESDIQPD
                     LDLRKPGQSSITTQRKEEDKVEILSGTFNGYTTNAPICMVIWNQDSDSRPYDKIKTIP
                     RPGHSDYPAMVKYGGYADYRGSGRFSGRLTASFVMAGAISKKLLKYVLGIETIAYTAK
                     IGEISIGNISFDKAKEGRYTNEVRCPHPHIAEKMKDSIMNARKSGNSLGGIVECVSTG
                     LPVGLGEPIFSSLESDLSKALFSIPAVKAVEFGSGFEGTKKTGYENNDLFIKEEGKVV
                     TITNNSGGILGGLSNGMPLTMRIGFKPAASIAKTQRTLDLSSNTLVDLQVPGRHDPCV
                     VPRAPPIVEGIVSIVLSDHAIKAGFIPSVLTGY"
     gene            5885..7261
                     /locus_tag="VF222_11115"
     CDS             5885..7261
                     /locus_tag="VF222_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7143016.1"
                     /translation="MVEQDNLDYLREEMRNITNQIIKLVHDRMEIAKKIGNIKQQLKI
                     EIEDEKVENDIKKTVLKLTKDIGMNVEFSGRLLNLLLSESVKLQNVQNNGNKSTTHIA
                     IFMKAKELEKNGKKIIHMEVGEPDYPPPLKVKEELANIYDNKRYHYTEISGISQLRNA
                     ISQRFLNIVPEQVLVTPGGRFAVFCAISSLLKSGDELINIEPSWPAYKECGDFIGAKT
                     RILKTTLEDKWTPNLKMLESLININTKMIILNYPNNPTGKVLDSHLLEKIVSIAREHN
                     LYILSDEVYSDYSFGKYNSILEFQYDKSIMVSSFSKTYGMTGFRVGYGIANNNIITKM
                     RKIQATSITSVAEPMQYSALAAIGNNIQTNIDSIQSRINLICKRLQLMKTEFIVPDGA
                     MYVYPKILSKNYSDDISLVHDLLEKGVAIAPGSGFGNSYKKFIRISACQSSKLLNDGL
                     DILEKSLK"
     gene            7276..8148
                     /locus_tag="VF222_11120"
     CDS             7276..8148
                     /locus_tag="VF222_11120"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX7143017.1"
                     /translation="MKQIAVIGAGGRMGSWLIKYFVNNKNFHIKAYDKNMYSIERHLN
                     VKIEIDFHSCVRDSDIVILCTPLRIIPQIIAECSKIMKRNSLLIDISSIKSKSFKSLS
                     KIQNYISPICIHPMFGPGASTTMRLKILFIPVRNYTKELQFVRQLFQNFEILPIENAI
                     KHDHIMAIVLGLNHFMNIIFADVISSNKYRDLEFYAGNTFRIQCILSESILNDDIDLL
                     NSLFIENPFIKKEIKNFHKKFLNYYQMINDKKQEKLAKQLKNLKSSIEEHHDLNSSYI
                     KMYKFINLLTKNKN"
     gene            complement(8799..9320)
                     /gene="cgi121"
                     /locus_tag="VF222_11125"
     CDS             complement(8799..9320)
                     /gene="cgi121"
                     /locus_tag="VF222_11125"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HEX7143018.1"
                     /translation="MVFLKIGNKYVYFHTVFFNQYNDFTIIDKLRKEFPDTFIQGLNF
                     NIVYNYKHLFEVLRISNLAKRRNIMCSHTIELDFLLRVMGTNQISQAIKEGGVIIDQY
                     ITFVVLGNKQQINKINKKLSLLFGNSHDKIFTDKIVLRKKIEHYKKKYNNGYFDEENT
                     IEFLREIAVLVMK"
     gene            complement(9338..9592)
                     /locus_tag="VF222_11130"
     CDS             complement(9338..9592)
                     /locus_tag="VF222_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7143019.1"
                     /translation="MITLKLLGGVKFAVGKNSIVIDKSQSTVKEIIEELKNESRDAKM
                     LNEKNLMVSVNGVDSSVTGGFKTVIKTGDVITIVTVIHGG"
     gene            complement(9598..9822)
                     /locus_tag="VF222_11135"
     CDS             complement(9598..9822)
                     /locus_tag="VF222_11135"
                     /inference="COORDINATES: protein motif:HMM:NF002242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HEX7143020.1"
                     /translation="MDRVKRLSTELLENYPDRFTTNFEENKKTILSLAIIRSKILRNK
                     IAGYITSKLTREADQEKSQSDYAESQMEES"
     gene            complement(10275..10829)
                     /gene="endA"
                     /locus_tag="VF222_11140"
     CDS             complement(10275..10829)
                     /gene="endA"
                     /locus_tag="VF222_11140"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HEX7143021.1"
                     /translation="MKTRKIQGELVENRIIIHDIENSRYLFGNGYYGKPLGIPKPKGI
                     NFESPLILDLIESYYLIQKGELQITLNDKVLPLKDLKMICKKQYDKFDSKYVVFQNLR
                     NQGYIVSPGIKFGCDFAVYERGPGIDHAPFLVQVLTKEEQITASTIVLAGRLATTVKK
                     QFIIAIPKLRERTVEFIGFEWWRA"
     gene            complement(10837..11229)
                     /locus_tag="VF222_11145"
     CDS             complement(10837..11229)
                     /locus_tag="VF222_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143022.1"
                     /translation="MYRRERELKKKALAVLHDEINKIKNPSIMILTLSLSIIKEEINN
                     VLSYFETLRNTEKIIEIKKKKILREVVDVYSYMLYRQVLRRAYSKISIKFFDRKEYNK
                     GFILLRQDGRMTIIVKDLGWQIKPFFLL"
     gene            complement(11302..12330)
                     /locus_tag="VF222_11150"
     CDS             complement(11302..12330)
                     /locus_tag="VF222_11150"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7143023.1"
                     /translation="MRIILIGYGIVGQSFTKLLLSKLVDLSNLYGMKPRIVACVDNKG
                     SAIDTSGLDIQRLLEIKKNKGTVGEYYRNKKSRLEPLQIIENIDAEIVMELTPTNLKD
                     GEPGLSHITSAMKYGKNVITVNKGPLSVAFPSLIELANFNGIMFKFSGTVGGGTPILE
                     FAKRCLKGDRIVSFRGILNGTTNFILSKMEEGLTFKKALKDAQIKGYAETVPSLDIDG
                     FDAAAKLVIMANWLMGMKVALEDVNRNGITKVKTQEVSKALKKGNAIKLIASCENKQL
                     VVKPIEVSKLDPICVNGTLNSVTFSLEYAGNQTIIGRGAGGIETASAVLRDMIEIREN
                     IINEKNLS"
     gene            12584..13351
                     /locus_tag="VF222_11155"
     CDS             12584..13351
                     /locus_tag="VF222_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7143024.1"
                     /translation="MIELFIILVLIGFGSGIIGSLIGVGGGIVMTPVLAYMGFPPAVI
                     ASSSLIAIFATSISSTLTYIRKKYINYFLGLKLALPAIPGSIIGGFFSNFISLEYFKV
                     YFAILLISVGVYIFFKNKIINKTLDTIPKPLFYLVLISGTFGAGIISSLFGIGGGIIF
                     VPILVLIYKMKMINASPTAQFTLLISTITGLLTHIILEHPEYSYGIALAFGAFFGAQL
                     GSRSIHLINENILSKMLSLSLIIIGINLVIDYIRSLK"
     gene            complement(13356..13544)
                     /locus_tag="VF222_11160"
     CDS             complement(13356..13544)
                     /locus_tag="VF222_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143025.1"
                     /translation="MEEKIHCNICNILIYGNNIDNHIMTPKHKNSKNRFIQQLYSVEV
                     DSKITNKSTYYVWKNNKN"
     gene            13697..15421
                     /locus_tag="VF222_11165"
     CDS             13697..15421
                     /locus_tag="VF222_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7143026.1"
                     /translation="MKYPKVVLSADRTLMSPYRGISLASFFGCAPALDFNRTKDSFWY
                     YILKNQVTPRVLFDFICNPIGHNNGRANFAPYGLRKLEASLLRDGYNPEDVVVAHPDY
                     IEQFIGPETQVVGTYEMDPLGMGPVTMTFTFGRKTMSYDELYNRDLHLRINAAKNRNG
                     SKAKVIVGASGTWQYNYDPAKIEEYGLYGIVEGDLGGIGPELDGVGARFFDAVINGEL
                     ETSNPFKKSEFKVEIKEFVKSDRKYHGRFFHYSDEPSLDEIPNIVNPSMHGMIEVMRG
                     CGRGCKFCDVTLRPLRYYPVEKVQKEIEINMKYGGLKNAWIQSDDIFVYGLNPRTTKN
                     MQPNREAIEELFKGIMDTGIEHTNPTHGTLAGAIADERLIPNVSKIIKSGPDNFIGIQ
                     CGLETGSIRLIGKYADRKLAPFKPAEWHWVVKEAVKTLNEHYWIPAFTLIMGLDNNES
                     LDDSWETIRLIHELETEQPDSKFTVTPLTFVPIGLLEKSSFFDIGNTMDPGKLGVMYK
                     TWQHNFKYGIQKFMSKVGRHNSIKSTFFSGLAKTLGGIPLSSMEKFARNKGPEHEKAI
                     ETIKANYW"
     gene            complement(15461..15628)
                     /locus_tag="VF222_11170"
     CDS             complement(15461..15628)
                     /locus_tag="VF222_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HEX7143027.1"
                     /translation="MPITDATKKQIAQQRRLFYKICFKCGGRNPISSERCRKCHSQKM
                     RLKNRNIGAKK"
     gene            16204..16764
                     /locus_tag="VF222_11175"
     CDS             16204..16764
                     /locus_tag="VF222_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7143028.1"
                     /translation="MICLTGMPGAGKSTVAEAIKDEGFVLITMGDCVRMEANRKNLQL
                     TDKNLGTLMLDLRNMYGQGAIAQLVINQINEKYYDPQYDKFIIDGIRSIHEVEILKTI
                     GTVKILAIHGSIFTRFVHLKNRSRSDAPSTLEDCRIRDKRELSVGVSEAIAFADEMIS
                     NNEITLDQLKKKAKSIAKKWICSLNG"
     gene            16816..17187
                     /locus_tag="VF222_11180"
     CDS             16816..17187
                     /locus_tag="VF222_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX7143029.1"
                     /translation="MEVSCKVNPTESEILIIKAINSLMEGVTLEFKYGDRIIGKTDNL
                     EVLNTIYNQVRDRSISSVLRRLLLSNLDQSSTWFYINKQVSTQGIIVLVDDEFESPLG
                     PIRITLKSNNIDRVIEWLCPE"
     gene            complement(17272..17898)
                     /locus_tag="VF222_11185"
     CDS             complement(17272..17898)
                     /locus_tag="VF222_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7143030.1"
                     /translation="MSTPTDILNKLFKETKPQDRLYPQPETKIQDIDSEEILNTNIYN
                     VMLNRRSQRKFETKEVENWKLDIIFAAADTAPTAGGFQGFDIYHIKNNDTKIKLVVAA
                     NNQPYVNAPVVLVFCMNPTRVKMQFPSEILKKFSIQDATLAAAYAQLAAHSLGLSSIW
                     IGMIDEKNVMETIGTDLIPSSILCIGYPKKVLQPKPKRALSELIHIIE"
     gene            18292..18382
                     /locus_tag="VF222_11190"
     tRNA            join(18292..18306,18323..18382)
                     /locus_tag="VF222_11190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18343..18345,aa:Arg,seq:cct)
     gene            complement(19042..19509)
                     /locus_tag="VF222_11195"
     CDS             complement(19042..19509)
                     /locus_tag="VF222_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143031.1"
                     /translation="MTNSSDKDDNRKNFDSHEYNFYNALKEDTTKIVNEIAKSQPTYI
                     QAISNLQMKYIEATKNIINSTLGFQKQVYESLDNNSTSTFTKQLGRQSNEITNNFIEF
                     NNINNKFISNVLEVAGDGIKNFTKTLDTFVKFNANVFEQWYSLFKNNQNFKRT"
     gene            19681..19809
                     /locus_tag="VF222_11200"
     CDS             19681..19809
                     /locus_tag="VF222_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143032.1"
                     /translation="MTYDYDAGTLRCQICAMEFPSRAEAEKHYKEIHSKEFTSIGE"
     gene            19917..20495
                     /locus_tag="VF222_11205"
     CDS             19917..20495
                     /locus_tag="VF222_11205"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEX7143033.1"
                     /translation="MNDSISFQENSSIPFEEVLKKYQEFWKKFSNVPKFSPFSLTDEA
                     SNVMKNLQDILLVTSKMQYYTLNYVTQINKTYTEALDKIPPNKSEYSIESEQDLKKYR
                     SFLLGTLEESFTNLFQSKEFGVLISDLMGTYSEYNKLFRNISNTYLLPLNLSSKDDVD
                     ILLKDIQELKREVRDLRKSIDIIMSEDNRIAK"
     gene            20485..>21569
                     /gene="phaC"
                     /locus_tag="VF222_11210"
     CDS             20485..>21569
                     /gene="phaC"
                     /locus_tag="VF222_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEX7143034.1"
                     /translation="MLNNSTTNPTNLEKHPSSSLSLDKYNQDLSEKIEKFTQIYQDPK
                     DIDVGITEHKIVKETNIYRLLHYNPIFKRPIKTPILVVYALINRSYILDLQGDKSWIK
                     NLLSQGFDVYLIDWKPPKSNDKYVNFDDYVNYFIDDCVNFIIDQGSIDKITLHGYCMG
                     ATMSVMYTTLHQRKIKNLVTLAPVIDTSKDLTVIGNFGRTMNVDKIIDSEGNMPPHLL
                     RTCFSALKPFKQGMNKYFTLLEHVDDKKFVQNFLRIEKWLYDVPPITGEVFKQWIKDI
                     YQNNLLAKNRLKLGSKLIDLSKISVPLLNIVAEEDHLVSPEGSAALNYLVSSIDNRLM
                     KFPTGHVGLIASSYSQLNVLPRVGKWL"
BASE COUNT         7563 a         3153 c         3382 g         7471 t
ORIGIN      
        1 caaataaagc atagttatgt tgataaaatt ttatcaggcg attgcaaaga aataatctct
       61 aaaattccta acggttcgat tcagttgact ataacttctc ctccctatag aaatgcaatt
      121 gattatagtg cacatacatc aaaaaatgga tcatattatc gaggaaaatc gaacattaat
      181 acaaaagaat atcttgagga gatgattgat atctttaata acaaaatatt caatgcaacg
      241 aaagaaggtg gttactgttg tatagttata gctaatgaag ttgtaaatgg taccctctta
      301 ccacttccac atatggtctt atcacaatta gtcgaaccat ttgggaagtg gaatcttcat
      361 gaagagatag tttggcacaa agtaacggga ggaactaata gatatggttc atttgttatt
      421 aatccttacc caaaatatta tagagcaaat atcatgcatg aactgatttt agttcttagg
      481 aaagggaacg ttagcagtgg aagaactgaa aggacagaaa cactacctgc tacacatgaa
      541 gaatggacaa aggaaatcgc aaattctgta tggcatatcg ctcctattcc tccaggctat
      601 atagaacatc catgcccctt tcccgaagaa ataccatata ggttaatgaa attatattcg
      661 tatgagggag atacaatttt agatcctttt aatggtagtg gacaaacaac taaagttgct
      721 ttccatttac agcgccgatt tatcggagta gatataatta atgaatatgt taagttagca
      781 cgatctcgat taatcgaacc aatacatatt agagcggaag cactaatagc aaattggaaa
      841 aaaattccct cccatcataa tttataaata tttactattt attgtatatt gaatttattt
      901 ttatgcttga ttagttgtaa tttttaatac ttagataata attactcatt gtgcatttat
      961 gcaattaaaa aatttatatt catttaaata aatgtattag tgaatagttt tactttggca
     1021 ggaagaattt gtgtttcaat cggtgcaaaa acaattaaag aattaacttc ttcaataaac
     1081 aaagccttag atttaagtga ttttttagag attaggtttg attttttaga taagtctgaa
     1141 atccttggtt ccatgaagat tattgaatca atcaaatcaa gatctgtatt tacgttaagg
     1201 tcacgtgaac aacaaggtaa atttaatgga aatgaatttg aaagacttaa aattctaaaa
     1261 atgttgtatg atgcaaatcc aatgttgtta gatgtagaat atgatacaat aactgcagac
     1321 caatcattaa acaattatct taaagacaag tctaacgttc taatttcatg gcataatttt
     1381 tccaatactc cttctgatga atatctagaa aaaaagattt acgatatgaa aagatttagt
     1441 aataatttaa aattagtcac aatggcaaca aatattacgg attctctaag attattaaat
     1501 ttatatgaaa aattccaaga tagtaattta attacatttg caatgggcga ttgtggtatt
     1561 atgtcacgtg ttttatgtac attttacgga aggcctcctt ttacttatgc ttcacttgaa
     1621 aaatctattg ctccaggtca gctatcggta atagacatga gaagaattta tgaaagaatt
     1681 aataaacatt ttttagataa tgtcatcacc aattaagaca tattgtataa taggtgatcc
     1741 tgtacagcat tcattatcgc ccttaatgca aaattctgcg ttttctgccc ttggaatcaa
     1801 aagctcttat atttcttttc gtgtaccctc tggagatctt aaaacatcaa tagaatcatt
     1861 aaaatcaatt ggaattgcag gttttaatgt tactattcct cataaaattt caatcctacc
     1921 ataccttgat gaattagata ttacagcttc taaatcaaat gctgttaaca cagtaaaaaa
     1981 tgatggagga aaattgattg gttataatac tgatctgtat ggttttattc agcctctcta
     2041 taaaagaaat atagatttca aaggaataaa gattttgata ataggtgctg gaggagcagc
     2101 tcatgcaata gtgacagctc tttcatatga aaaagaaata gctgagatta ttgtagttaa
     2161 tcgttcatcc cacaatgcgt caaagctagt acaacacgga ttgaaattag gattaaatac
     2221 tcataaagaa gatttcgata ctctttctaa acttgcttta caatcagatt taatcgtaaa
     2281 tgctacttca attggattaa acaatgaaag tagtccaata gaacgagatt atataagtcc
     2341 aaactgtatt gtgtatgata ttgtatacaa acccatagtt actgatttac taggaaaagc
     2401 taaggaagtt catgccagag ttgtatatgg atatgaaatg cttattgcac aaggagcaca
     2461 agcattcaag atttggactg gtttcgatgc tcctatcatt gctatgaaaa aggccttatt
     2521 cggcatattt ggagaaccca catgacatct atttcaacaa aagctacagt tcatggggca
     2581 atttcaatag tcaatgctat tgctactggt aaaggttctg ctttgggaat ttcactcgag
     2641 acaaccgcag aaatccatat gcgtaaagga aacgggaaga tcattcatca taacaggaat
     2701 agcgaattgt tcagatatat tatccataat agcattccta aaaaaatact tcaggataat
     2761 gatatttttt taactatatg ctctgatata cctattggat ttggattgaa aagttctagt
     2821 gctgtttctt cagcagtatc attagcatgt tatggagtag tgaaagatac tattaatgat
     2881 tttaaagttt tggataccgc agtagatgca tcacgcaaag ctaaaataac aataacgggc
     2941 gcttatgatg actctactgc ttgttatttc ggtggttttg taattacaga taattattct
     3001 aacaaattat taagaagaga aaatggaccg attgatttgt tatctcttgt tcttctacct
     3061 aataattccg tacgaaaaag tcatttaaaa ttaaaaattt tatcagaatt tttcgaacaa
     3121 gcacttctct cagcagaaaa atctgatttt tggaaggcta tgactctgaa tgggcttttg
     3181 gtatcttctt tgatgggtta tgattatact ccgatccttc tgtccataga aaatggcgct
     3241 ttatcagcct caatttctgg aaatgggccg tcaatagccg ttattattga gaaaaagaat
     3301 cttactacaa ttagaagtat cttagaaaat tatggaaaaa cactagtttg caaaattaat
     3361 aataccaaag cgagcataga aaaaaaaatt gactaaacta gaaatcagta aatctttttt
     3421 agaaggtaat ataaaatgcc cgccaagcaa aagttatact cacagagcaa ttgctattgc
     3481 ctctcttgca gatgggaaat ccacaattag taatccactt cttagtagag ataccatagc
     3541 aaccatttct ggttgcaaaa tgtttggaat aataatagga aaatctaatg aaaatgttaa
     3601 ttgtatccaa atactaggaa aaaatcaatt tgaaacgcca aaggatataa ttgatgcgaa
     3661 aaactcggga acaaccatta gaattttaac ttcaatggcc gggctggtaa aaaatggtta
     3721 tactattctt actggagatg aaagtttaaa gacaaggccg atgcaacccc taatttcagc
     3781 tctaaatcag ttaggtgtcc atgctttttc ttcaaatgaa aaaaatactc ctccattaat
     3841 cgtcaaagga ggtggaataa agggtggttt ggtttcaatt gaaggaggga tttcaagtca
     3901 atttatttct tcattattga tttcctgtag ttatgctctt actgatgttg aaataagagt
     3961 taaaggaaaa caggtatcta tcccgtacat cacctcaact atagaaacaa tgaaaaaatt
     4021 tggtatatcc attgaacaca gtaaagaata tttgaattat tatatatcta attataaata
     4081 cagaccaacc gattttacta tccctgggga tttttcttct gcatcactta ttatagcagc
     4141 aggtgtttta tgtggaggaa aaatagcaat aggaggcatg aattttcatt atccacaagg
     4201 agacatggca ataattgata ttgttagaca aatgggtggg gatattaaag taaataaaga
     4261 aaaaggagaa gttattatat atggatctaa cagtcttgat ggaatagaat gtaatcttgt
     4321 gaattgtcct gatctcttac cggtagtttc tattctttcc ttaaaagcaa gaacacctat
     4381 gagaattttt ggaatttctc atgctagatt caaggaaact gatagagttt caattattac
     4441 taacgaattg agaaagatgg gagtggatgt tagtgaaaaa gaagatgaag tacttattaa
     4501 tccagtcaaa ggtttgaaaa atgtagaatt tgattctcat aatgatcata gactttttat
     4561 gtcatttaca atcgcatcta tgctgacgaa taaatcaatt gtgaaaggag ccgaatctat
     4621 agatgtatct tatcctgcct tcataaacga aattaaaaga cttggtgcaa atataacttt
     4681 accaaaataa attgatgaca ctgaaattac aaaaaacaat attctaattc tttaaaagtc
     4741 actattatat aggactacta tatctttggg tacgatgtta tgggtggaaa cagtctaggc
     4801 gaacgattca tagttactag ttttggtgaa agtcatggta gatgtgtagg gtctatttta
     4861 gatggttgtc cagcaggtct cccattgaag gaatccgaca tacagccaga tttagatcta
     4921 cgaaaacctg gacaatctag tattacaacg caaagaaaag aagaggacaa ggtagaaatt
     4981 ctttcaggta catttaatgg ttatacaacc aatgcaccta tatgtatggt aatttggaat
     5041 caagattcgg attcgagacc ttatgataag ataaaaacaa tcccaagacc tggacattct
     5101 gattatcctg caatggtaaa gtacggagga tatgcagatt atagaggtag tggtagattt
     5161 tctggtagat tgactgcaag tttcgtaatg gcaggagcaa taagtaagaa attgttgaaa
     5221 tatgttctag gaatagaaac aattgcttat actgcaaaaa taggagaaat atctattggc
     5281 aatatttctt ttgataaggc aaaggaaggc cgttatacta atgaagtaag atgtcctcat
     5341 cctcatatag cagaaaagat gaaagattca attatgaatg caagaaagtc aggaaattct
     5401 ttagggggta tcgttgaatg tgttagtacg ggattaccag taggactagg agaacctatt
     5461 ttttcatcat tggaatccga tttaagcaaa gcattattta gtattcctgc tgttaaagca
     5521 gtagaatttg gttctggttt tgaaggaaca aaaaagacag gttatgaaaa taatgattta
     5581 tttataaaag aagagggaaa agtggtaaca atcactaata attctggtgg tatattaggt
     5641 ggcttatcaa atgggatgcc tcttacaatg agaataggat ttaaacctgc agcctccatt
     5701 gcaaaaactc aaagaacatt ggatttatct tcaaatactt tagtagatct tcaggttcct
     5761 ggcaggcatg atccgtgtgt tgttccacgc gctcctccta ttgttgaagg gattgtttcc
     5821 atagttttgt cggatcacgc aattaaagct gggtttattc cgagcgttct aacgggttat
     5881 tgaaatggtt gaacaagata atctggatta tttaagagaa gaaatgagga atattacaaa
     5941 ccaaattatt aaattagtcc atgatagaat ggaaatagca aaaaaaattg gtaatatcaa
     6001 acaacaatta aaaattgaaa tagaagatga aaaggtagaa aatgacataa aaaaaactgt
     6061 cctgaaactt acaaaagata taggaatgaa tgttgaattt tctggaagac tattgaatct
     6121 tcttctttcg gaatctgtaa aattacaaaa tgttcaaaac aatggtaaca aaagtactac
     6181 tcatattgct atatttatga aagctaagga acttgaaaag aatggaaaga aaataatcca
     6241 tatggaagtc ggtgagcctg attatcctcc tcctttaaaa gttaaggagg agttagcaaa
     6301 tatttatgac aataaaagat atcattatac tgaaatttct ggtatatcac aacttcgtaa
     6361 tgcaatatct caaagattct tgaatattgt accagaacaa gttttagtta ctccaggggg
     6421 taggtttgct gttttttgtg caataagctc gctgttaaaa tcaggggatg agttaattaa
     6481 tattgaaccg tcatggcctg cttataaaga atgtggagat ttcataggtg caaaaacaag
     6541 aatactaaaa actactctag aagataaatg gacaccgaat ttaaaaatgc tcgagagcct
     6601 aataaatatt aataccaaaa tgataatctt aaactatcct aataatccaa caggaaaagt
     6661 acttgattct catttactgg aaaaaattgt ttctatagca agagaacata atctttatat
     6721 attaagcgat gaggtttatt ctgattattc ttttggtaaa tataatagta ttctagaatt
     6781 ccaatatgat aaaagtataa tggtatcatc tttttcaaag acttatggaa tgacaggatt
     6841 cagagtaggt tatggaatag caaataataa tataataaca aaaatgcgca agattcaggc
     6901 tacatctatc acatctgtag cagaaccaat gcaatattca gctttagccg caatagggaa
     6961 taatattcaa acaaatattg attccatcca atcccgaata aatcttattt gcaaaaggtt
     7021 acaattaatg aaaacagaat ttattgttcc tgacggtgca atgtatgtct atccaaagat
     7081 tttatctaaa aattattcgg atgatatttc gttagtgcat gatttattgg aaaaaggagt
     7141 agcgatagcg ccaggaagtg gatttggaaa ttcttataaa aaatttatta gaatttccgc
     7201 ttgtcaatca tcaaaattgt tgaatgacgg tttggacatt ctggaaaaat ctcttaaata
     7261 ggtacctaaa ttgatatgaa acaaattgca gttattggtg caggagggag aatgggttct
     7321 tggctaatca agtattttgt caataataaa aactttcata taaaagcata tgataaaaat
     7381 atgtactcaa tagagcgaca tctaaatgta aaaatagaaa ttgattttca ttcatgtgtt
     7441 agagattctg atatagtaat tctgtgtact ccattgagga ttataccaca aattatagca
     7501 gaatgttcca aaataatgaa acgaaattca ctattaattg atatctcttc tattaaaagc
     7561 aaatctttta aatctctttc taaaattcaa aattatattt cacccatttg catacaccct
     7621 atgtttggtc caggggcatc taccaccatg cgcttaaaaa ttttgttcat tccagttaga
     7681 aactatacaa aagagctaca gtttgtaaga caactatttc aaaatttcga aattttacct
     7741 attgaaaacg caattaaaca tgaccatatc atggctatag tcttgggatt gaatcatttt
     7801 atgaatataa tttttgcaga tgttatttca tcaaataaat acagagattt agaattttat
     7861 gctggaaata catttaggat acaatgtatt ttatcagaaa gtattctaaa tgatgatatc
     7921 gaccttctta attctttatt cattgaaaat cccttcatta aaaaagaaat taaaaacttt
     7981 cataaaaaat tcttgaacta ttatcaaatg attaatgata agaagcaaga aaaattagca
     8041 aaacaattaa aaaatttaaa atcttccatt gaagaacatc atgatcttaa ttcatcctat
     8101 atcaaaatgt ataaattcat aaatttacta actaaaaata aaaattaaaa tattctagac
     8161 ctgatcagag attttacata ttgtgacttc tctttaaccg gctaatattc taccaaaaac
     8221 actcaagttt caacaatagg gattaaaaga ctagttattt cagtttcaaa ttttagagag
     8281 ttagattcat tattcttttt tgactaatgt ttttagtaat attgatttct tcattcattt
     8341 catctatttg attacaatgt tttcataatt agggaaattt tcatcctata tgttccaaac
     8401 tattcgtctc ttcggaaaaa taggataagt taatttttgc gtcgtgtaca tcattattgt
     8461 gataaaaaaa tattatgata aagtttctct tatcatatac tttgagaaaa aaacttgatt
     8521 ttaaatacca tttaattagg acacttatta tgtacaaaaa aaaacgagtc ttatatttgc
     8581 agttataaat ccaaaaaatt tttcttttta taatacaaat ggctattaag gaaaaatcaa
     8641 tgaatttaaa tattttcaaa ttaatatagc caagtaatac tcacatcgcc aattaaaggt
     8701 tatacacata gtattaactt attatcaacg tgatcgatta caagaaaatt gttacaaatc
     8761 aacccctcat attatacaag aataaatcta atagaaatct atttcataac tagaactgct
     8821 atttctctta gaaattcaat ggtattttct tcgtcgaaat agccattgtt atattttttt
     8881 ttatagtgtt caattttttt tcttaaaact atcttgtctg tgaaaatttt atcatgggaa
     8941 ttaccaaata gaaggctaag ttttttgtta atcttgttga tctgttgctt atttcctaat
     9001 acaacaaaag taatatactg atcaattatt actccgccct ctttaattgc ttgagatatt
     9061 tgattcgttc ccataactct caacaaaaaa tctaattcta ttgtatgtga acacattata
     9121 ttccttcttt ttgctaaatt tgatattctt aaaacctcaa aaagatgttt gtagttataa
     9181 actatgttaa aatttaaacc ttggataaaa gtatcaggaa actcctttct caatttatca
     9241 attattgtaa aatcattata ttgattgaaa aatactgtat gaaaatagac atacttattt
     9301 cctattttta aaaaaaccaa atttgttttc taattgttta accgccgtgg ataactgtaa
     9361 ctattgtaat cacgtcccca gttttgataa ctgtcttgaa accaccggta acagaagaat
     9421 cgactccatt aacagatacc attaaatttt tttcatttaa cattttggca tctctggact
     9481 cattttttag ttcttcgata atctctttca cggtggactg tgatttatca atgactatag
     9541 aattctttcc cacggcaaat ttgactccac ctaagagttt taacgtaatc acagtgttta
     9601 actttcttcc atttgactct ctgcgtaatc tgattgagat ttctcctgat ctgcttctcg
     9661 tgtaagctta gatgttatat atcctgcaat cttgtttctt aaaattttag atcttattat
     9721 agctaaactt aaaatagtct tcttattttc ttcaaaattt gtagtaaatc tatcaggata
     9781 attttccaaa agttcagttg ataatctctt tacgcgatcc atataataaa tatagaaaaa
     9841 tttgaatatt ttatctttgt gattgtgatt ataatttctt aattataact taaaaagtat
     9901 tatcattata tgtgtgttct ataaaaaaat tttaaggcat ttttacgtgc attgtaatag
     9961 tcctgtaaaa tataattatc tggtattcat attattgcgg ttagtattat taagatattt
    10021 tactagatcc acatcaatga aggtaggtaa ctattattat ccctaaatgt gtaactataa
    10081 aaaacgtata taagcagatc atttcttagt ttttcttaat ttattatata tgatctcttt
    10141 actattttat aagaccaatc atgtatattt ttaattagta ataaaaaaaa caatatctgt
    10201 taagaccatt aaatttttgt caataatctt ttcatcataa atttttgatc aatattgtcc
    10261 agttctaatg attattaagc tctccaccac tcgaatccta tgaattctac cgttcgttct
    10321 ctcaatttag gtattgcaat aataaattgt ttttttacgg tcgttgctaa tctcccagca
    10381 agaacaattg ttgacgcagt aatttgttct tcctttgtta aaacttgaac aagaaaaggt
    10441 gcatgatcga tcccaggacc tcgttcatat accgcaaaat cacatccaaa ttttatgcca
    10501 ggagagacaa tatatccttg gttccttaaa ttttgaaaca ctacatattt tgagtcaaat
    10561 ttatcatatt gttttttaca aatcatcttt aaatccttta atggtaggac cttatcgttt
    10621 aaagttattt gtaattctcc tttttgaatt aaataataac tttcaattag atctaaaatg
    10681 agaggggatt caaaattaat tccttttggt tttgggattc ctaatggctt gccataatat
    10741 ccattaccaa atagatatcg agaattctca atatcatgta ttattattct attttctaca
    10801 agttctcctt gtatttttct agttttcatt atttaattat aaaagaaaaa aaggtttgat
    10861 ttgccatcca agatctttga caattattgt cattcttcca tcctgtctca aaagaataaa
    10921 tcctttgtta tactcttttc tatcaaaaaa ctttatagaa atcttgctat atgctcttct
    10981 taagacttgt ctatatagca tataactata gacatctact acttctctta atattttttt
    11041 ttttttgatt tcgattattt tttcggtgtt tcttagagtt tcaaagtatg atagaacatt
    11101 attgatttct tctttaatta ttgaaaggga tagagtaaga atcataattg aaggattctt
    11161 aatcttatta atttcatcat gaagtacagc taatgctttt ttctttagtt ctctttccct
    11221 cctatacact aaatcaaagc gtttctatca gggtaataat tctttttaca tttaaaattt
    11281 tgatacaaat ttttatagaa attaagacaa atttttttca ttgatgatat tttctcggat
    11341 ttctatcata tctcttaata cagcactagc agtttctatt ccaccggctc ctcgtcctat
    11401 tattgtctga ttccctgcat actccaatga aaaggtgact gaatttaaag ttccattgac
    11461 acatataggg tccaactttg acacctctat aggcttaacc acaagttgtt tgttttcaca
    11521 gctagcaata agtttgatcg cgtttccttt ttttaatgct ttggaaactt cctgagtctt
    11581 cacttttgta atgccatttc tattaacatc ttctagtgca actttcatac ccattaacca
    11641 gtttgccatt atgaccaatt tcgcagcagc atcgaaccca tctatgtcta aggaaggaac
    11701 agtttcggca taacccttaa tttgagcatc ctttagggct tttttgaatg tcaacccttc
    11761 ttccatttta ctcaaaataa aattagttgt tccattaagt atacctctga aagaaacaat
    11821 tctatcccct tttaaacacc tcttagcaaa ttctagaatt ggagttcctc cacccacagt
    11881 tccactaaac ttaaacatta ttccgttaaa gttagctagt tctataaggg atggaaaagc
    11941 tacagataat ggtcctttat tgactgttat tacattttta ccatacttca tagctgaagt
    12001 tatatgagat aaacctggtt caccatcctt tagattagtt ggagtcagtt ccataactat
    12061 ttctgcatcg atattttcta ttatttgtaa aggctctagt cgtgattttt tatttctata
    12121 atattcacct acggttcctt tatttttttt tatttctaaa agtctctgaa tatctagacc
    12181 agaagtatca attgccgagc ctttattgtc aacgcacgct acaatcctag gcttcatccc
    12241 atatagatta gataaatcta ctaatttgga aagtaatagt ttggtaaaac tctgacctac
    12301 aattccatat ccaattaaaa taattctcaa ttctaaataa caatatgata aataatgata
    12361 tacttatatc ttataagtaa aagtattaga tgacttctaa ttgagttgta aaagaaactt
    12421 gaattattag taatacatcc gtctgtactt gtttgtagaa tgaaataggg gtggattgta
    12481 actttttgtt gatagttgaa caattggctc tgctaactca agaagtaaag agaataaatt
    12541 taacttgtct accttatgaa agttaaggta taggaagagt aatttgattg aactattcat
    12601 tattctcgta ttaattggat ttgggtctgg tataataggc tcgcttatag gagtaggcgg
    12661 cggaatagtc atgacaccgg tattggcata tatgggattt cctcctgctg tcattgcaag
    12721 ttctagtcta attgccatat ttgcaactag tatttcatcg acattaacat atattagaaa
    12781 aaagtatatt aattatttcc ttggacttaa actagcttta ccggctatac caggatcaat
    12841 tattggagga tttttttcta attttatatc cttagagtat tttaaggttt attttgctat
    12901 tttgttgata tcagttggag tatatatttt ttttaaaaat aaaattatca ataaaactct
    12961 agatacaatt ccaaaacccc ttttctattt agttttaatt tctggaactt ttggtgcagg
    13021 tattatatca agtctttttg ggataggagg aggaataatt tttgttccta ttctagttct
    13081 tatttataaa atgaaaatga tcaatgcgag tcctacagct cagtttacac ttttaataag
    13141 tacaataaca ggacttttga ctcatattat tttagaacac cctgaatata gttatggcat
    13201 tgctcttgct tttggtgctt tttttggtgc acaattaggt tcaagatcaa ttcatcttat
    13261 taacgaaaat atattgagta aaatgctctc tttgtcttta ataattatag gaattaattt
    13321 agtaatagat tatataagaa gcctaaaatg acattttaat ttttgttatt tttccatacg
    13381 taataggttg atttatttgt tatcttgcta tcgacctcta cagaatatag ttgttgtata
    13441 aacctgtttt ttgaattttt atgttttggt gtcataatat gattatcaat attattacca
    13501 taaattaaaa tattacaaat gttacaatga attttttctt ccataacgat atctctttta
    13561 atacataaat ataacatttt attttcgaac tggacattta actttagatt acaattccct
    13621 tgaataaatc tatgtgaaag gtgggaaaag ttaaaattca tccctctgtc aaattcaatg
    13681 attaggatta attaaaatga aatatccaaa agttgtatta tctgctgatc gtactttaat
    13741 gtctccctat cggggaatct ctctagcttc gttttttggt tgtgctcctg ctttggattt
    13801 caacagaact aaagattcat tctggtatta tattttaaaa aatcaagtta ctcctagggt
    13861 tctttttgat ttcatttgta atcctattgg acataacaat ggtagagcaa actttgctcc
    13921 ctatggattg agaaaactag aagcatctct attaagagat ggttataatc cagaggacgt
    13981 tgtcgttgct catcctgatt atatagagca atttattggg ccggaaactc aagtagtagg
    14041 aacctatgaa atggatccct tgggaatggg tccagttact atgactttta catttggaag
    14101 gaagactatg tcttatgatg aattatataa tagagactta catttgcgta taaacgctgc
    14161 taaaaataga aatggtagta aagcaaaggt aatagtaggt gcatctggta cttggcaata
    14221 taactacgat cctgctaaaa tagaagagta tggactatat ggcattgtgg aaggagatct
    14281 tggaggaata ggacctgaat tagatggagt aggagcaaga ttttttgatg ctgtcattaa
    14341 tggcgaatta gaaacctcta atccgttcaa aaaaagtgaa ttcaaagtgg aaattaaaga
    14401 atttgtgaaa agcgacagaa agtatcatgg ccgtttcttt cactattctg acgaaccatc
    14461 tttagatgaa atacccaata ttgtcaatcc cagtatgcat ggaatgattg aggttatgcg
    14521 aggatgtggg cgtggctgca agttttgtga tgttacttta cgtccattaa gatattatcc
    14581 agttgaaaaa gtacaaaaag aaatagaaat aaatatgaaa tacggaggtc ttaaaaacgc
    14641 atggattcag agtgacgata tttttgttta tggtcttaat cctcgaacta ctaaaaatat
    14701 gcagccaaat agagaagcaa ttgaagaact ttttaaaggg attatggata caggaataga
    14761 acacacaaat cctactcatg gaacgttagc aggtgctatt gctgatgaaa gacttattcc
    14821 aaatgtttcg aagataatta aatctggccc agataatttc atcggcattc aatgtggttt
    14881 agaaacaggt agcataaggt taataggaaa atatgctgat agaaaattag ctccatttaa
    14941 accagctgag tggcattggg tagttaaaga agcagttaaa accttaaacg agcattattg
    15001 gattccggca tttacgctta ttatgggatt agataacaat gaaagtctcg atgatagctg
    15061 ggaaacaatc aggcttatac atgaactaga aacagagcaa ccagattcga aatttacagt
    15121 aacaccactt acttttgttc caattgggct attggaaaaa tctagctttt ttgatattgg
    15181 aaatacaatg gatcccggaa aattaggagt aatgtacaaa acctggcaac acaattttaa
    15241 atatggtata caaaaattta tgtcaaaagt aggtagacat aattctatca aaagtacttt
    15301 tttctcaggt ttggcaaaaa cattaggtgg aataccatta tcatcaatgg aaaaatttgc
    15361 aagaaataaa ggacctgaac atgaaaaagc tattgaaaca attaaagcga attactggta
    15421 aatcaaaaat atttaaataa aataagcata aacaacaata ttattttttg gctccaatgt
    15481 ttctattctt taatctcatt ttttgactat ggcattttct gcatctttcg gaagaaatag
    15541 ggtttctacc tccacattta aaacatattt tataaaataa tcttcgctgt tgtgcaattt
    15601 gttttttagt agcatcagtt attggcataa actccaatag aataattaag ttatttgtat
    15661 ttttcttttt cactattagt tttactaagc aaaattaatt taatatctaa caattattgg
    15721 ttatttaatt tacattgtgg aattaaaaac tgctattaca tatgtattac atatgtagga
    15781 tctttttatt actgatgtat aaataagtgc gaatttcttt tggaatattg atttgttttc
    15841 tgaattaaaa tcattctact caatatatat cgtcttccat ttctatttat attgtatcaa
    15901 aactttatta aatgaatgag gtcgccgtat atctttggac cttttttaaa ttcttgtctt
    15961 tgttttttgg tgtttactaa aaaataaaat atttcgaatt aggaattgct agttaatcat
    16021 ctatgagtta aaaattgaga tggcatactt tcaataagta aagctagata ctagaaatag
    16081 taggtagcaa ttaattacca tttaaaatat aatgtgaata agcaagacaa agcaagacaa
    16141 agttgataat atccttgcat tgcattacaa taattaggtg aactatatat caaacaaaca
    16201 tttatgattt gtttaactgg aatgcctggt gcaggaaaat caacagtagc agaggcaata
    16261 aaagatgaag gttttgttct gataactatg ggagattgcg ttcgaatgga agctaacaga
    16321 aaaaacctac agttaactga taaaaatctg ggaaccctga tgttagattt acgaaatatg
    16381 tatggtcaag gtgcaattgc tcaactggtc attaatcaaa taaatgaaaa atattatgat
    16441 cctcaatacg acaaatttat aattgatggt ataagaagta tccatgaagt cgaaatactg
    16501 aaaacaatcg gaacagtgaa aattttagca atacatggat caatctttac taggttcgta
    16561 cacttaaaaa atcgttctcg atctgatgct ccatctactc tggaagattg cagaattagg
    16621 gataaacggg aattatcggt tggagtaagt gaagcaattg catttgcaga tgaaatgata
    16681 tctaataatg aaataacatt agaccagtta aagaaaaaag ctaaatccat agcgaaaaaa
    16741 tggatatgtt cattaaatgg ttaagtcatt acagaaaata ttagaaaaac aaaatttcca
    16801 actttaaaca tagaaataga ggtatcctgt aaagttaatc caacagaatc agagatttta
    16861 ataatcaagg caattaattc tcttatggaa ggggtaactt tagaatttaa gtatggagat
    16921 cgcataattg gtaaaactga caacttagaa gttctgaaca ctatttataa ccaagttcgg
    16981 gataggtcaa tttcttctgt gcttcggagg ttattacttt ctaatttaga ccaatcttct
    17041 acctggtttt atattaacaa gcaagtatca actcagggaa ttatagtatt agttgatgat
    17101 gaattcgaat ctcctcttgg accaattcga ataactctca agagtaataa cattgataga
    17161 gttatagaat ggctatgccc agaataataa atcatcaaat taatctactc taaagttttc
    17221 tacttaatac aaacctaatt tattattctc tttatcaaat aatttcatta attattctat
    17281 tatgtgtatg agttcggata atgctcgttt tggtttgggt tgtaaaacct tttttggata
    17341 cccaatacat aaaatggaag atgggataag gtctgtacct atggtttcca tgacgttttt
    17401 ctcatcgatc attccaatcc aaatagaact aagacccaat gaatgagcag ctaattgagc
    17461 atatgcagca gcaagtgttg cgtcttgtat agaaaatttt tttaatattt cggatggaaa
    17521 ttgcatttta actctagttg ggttcataca gaataccaat actacaggtg catttacata
    17581 gggttgattg tttgctgcta caactaattt tatttttgtg tcattgtttt ttatatgata
    17641 tatatcaaat ccttggaatc ctcctgcagt aggagcagtg tctgcagctg caaaaattat
    17701 atctaacttc cagttttcaa cttctttagt ctcaaatttt ctttgagagc gtctatttaa
    17761 catcacgtta tagatattag tatttaaaat ttcttcggaa tcgatgtcct gaatcttagt
    17821 ttctggttgg ggatacagtc tatcttgtgg cttagtttcc ttaaataact tgtttagtat
    17881 atctgttggg gttgacatat ttttacaagc atcccattat tagaaaactt tttcggatat
    17941 ttattatata tatttttatt gtattacaaa ttaactttaa atcacagatt ctatgtaaaa
    18001 taaaataacc gccatcctaa atcttgtatt atttacttat ttcttttaat tatttgtgct
    18061 gtcaaacgct ttatttttaa taagtagttt tttttcacat tctaagtatt ttatatctct
    18121 tataattgtt tataaatgat aacaatataa aattaacggt ttgatagtaa acttacaata
    18181 ggatctaatc gtatttcaat aatctagtta gtatttcaat aaagacatct tctttcttcg
    18241 attttacttc ttaaactctt aaattaatgc ttattgttgt tttattgaca agggctcgta
    18301 gcgcagacta taaaaggcgc aaaatggaca gcgcggatgc ctcctaagcg tcaggtcgag
    18361 ggttcgaatc cctccgagcc cgtttttgcc aattccatta ttattaagcc caactttagg
    18421 atatttaaga tatgggaatg gcagaaagac aacaaaagaa ttcccactct aaccaatagt
    18481 gagcccatat taatcatttt gtatggcctg aaagcctcag aaaccacagg acaagatctt
    18541 aagtttgaga tttgtttgct cccattcact tcgtcttgtc ataacatttt acaagaaata
    18601 accaacattt gtacatagtg aaacgtgaaa taaaaattcc tagcatgtat aaatattgca
    18661 tctggtaaaa atgttgctaa aaataataat taagtattaa ttatcctgac aattatgatt
    18721 attaatcaag tatgtaattt atttttgaga tgttatcttt acttaaggta aacgaatagg
    18781 atgataataa taatgatatt caaaataaac ttatttttag ttataatgat ctttattttt
    18841 aataattgaa aaacatgttt agcaagatat aaagtgcttt atctaacctc aaccaaggaa
    18901 tatcatttag agcctttgta ttcattcatt tattttttat agtgagatat tagtagatat
    18961 cttttgggtt gagattagaa attcatgaga atcggtattt tggtcctttt taaaataatt
    19021 ataggaaatg aagtagataa gttatgttct tttaaaattt tgattatttt tgaataaaga
    19081 ataccattgt tcaaaaacat ttgcattaaa tttcacaaaa gtatctaaag ttttagtaaa
    19141 attttttatg ccgtctcctg ctacttccaa gacattcgag ataaacttat tatttatatt
    19201 gttaaattct ataaaattat ttgtaatttc gtttgattgt ctgccaagct gttttgtaaa
    19261 agtggaagtt gaattgttat ctagtgactc gtatacttgt ttttggaacc caagggtaga
    19321 attaattata ttttttgtag cttcaatgta tttcatttga agattggaaa ttgcctggat
    19381 ataagttggc tgtgattttg ctatctcatt tactatcttt gtggtatcct ctttaagtgc
    19441 attgtaaaaa ttatattcat gagaatcaaa gtttttacga ttatcatcct tatcactaga
    19501 atttgtcata tttactcaat atatttgagg taatcaaact atatataatt atggggacgc
    19561 ccacttcaac tattaagtta tagtattact ttatatatca aaaactaaca acctttagta
    19621 tacagtgaaa aacaaacctt attagtataa ataatatata gttttattgg tgctacttat
    19681 atgacttacg attacgatgc tggaacgtta agatgccaaa tttgtgctat ggaatttcct
    19741 tctagagcag aagcagaaaa gcattataaa gaaatccatt caaaagaatt tacgtccatc
    19801 ggagaataaa aaaagtacat ttatacactt ttattaatta ttaaaaaaac gtaacaagtt
    19861 atattaaatg aaattaaaca aaaagttata taatttttaa tacatactta tggtagatga
    19921 atgatagtat atcttttcaa gaaaattcta gtattccgtt tgaagaagta ttaaagaaat
    19981 atcaagaatt ctggaaaaaa ttctctaatg ttccaaaatt cagtcctttt tctttgacag
    20041 acgaagcgtc aaatgttatg aaaaatttac aagacatttt actagtgact tcaaaaatgc
    20101 aatattatac ccttaattat gtaactcaaa ttaataaaac atacacagag gctttagata
    20161 aaataccacc aaataaatct gaatattcaa ttgagtctga acaagactta aagaaataca
    20221 gaagtttcct tcttgggact ctagaagaat cgtttactaa cctgtttcag tctaaagaat
    20281 ttggagtcct tatttctgat ttaatgggta cttattcgga atataataaa ttattcagga
    20341 atatatctaa tacctaccta ttacccttaa atctatcttc caaagatgat gttgatatac
    20401 ttctcaaaga tatacaagaa ttaaaacgag aggtccgaga tttaagaaaa agtatagata
    20461 ttataatgag tgaagataat agaattgcta aataatagta ctactaatcc tacaaattta
    20521 gaaaaacatc caagtagctc tctttcttta gataaatata atcaagattt atccgaaaag
    20581 atagaaaaat ttacacagat ctatcaagat cctaaggata ttgatgtggg tattactgag
    20641 cataaaatag taaaagaaac aaatatctat agattattac attacaaccc catttttaaa
    20701 cgaccaataa aaactcctat cttagttgtg tatgccttaa ttaatagatc atatatattg
    20761 gatttgcaag gtgataaaag ttggattaaa aatcttttat ctcaagggtt tgatgtttat
    20821 cttattgact ggaaacctcc taaatcaaat gacaaatatg taaattttga tgattatgta
    20881 aattatttca tagatgattg cgttaacttc attattgatc aagggtctat agataagata
    20941 acattgcacg gctattgcat gggcgccaca atgtcggtaa tgtacacaac attgcatcaa
    21001 cgtaaaatta agaatctggt gactttagca ccagtaatag atacgagcaa agatctcaca
    21061 gtaatcggaa attttggtag aactatgaac gttgataaaa taatcgattc tgaaggaaat
    21121 atgcctccac acctacttcg tacttgcttt tcggctctta aaccattcaa acaaggcatg
    21181 aacaaatact tcacactttt agagcatgta gatgacaaaa aattcgttca aaatttctta
    21241 agaatagaaa aatggctata cgatgtacct cctataacag gagaggtatt taaacaatgg
    21301 atcaaagata tttaccaaaa taatctcctg gccaaaaata gattgaaatt aggatctaaa
    21361 ttaattgact tgtcaaagat ctctgtccca ttgttaaaca tagtcgctga agaagaccat
    21421 ctagtttctc cagaaggtag tgcggcacta aactacctcg tctcaagtat cgacaataga
    21481 ttaatgaaat ttccaactgg acatgttgga ttaattgcta gtagctattc acaactaaat
    21541 gtacttccaa gagtaggaaa atggttgtt
//
LOCUS       DASVEV010000255         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_716143, whole genome shotgun sequence.
ACCESSION   DASVEV010000255 DASVEV010000000
VERSION     DASVEV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716143"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VF222_11215"
     CDS             complement(<1..444)
                     /locus_tag="VF222_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143035.1"
                     /translation="MLKISSFLFGIIITSIILFSANSPFISIYAQDTQYYNNNTNENN
                     NSSLLNELENIKNILESKVTKLATALQIAGSLPQILQPPDVNLIDPKVKGIPEDADLE
                     KRKIGKILLEQFNELFSVVFLLNNGDVYFDEPFERQLNRTATNLSF"
     gene            complement(935..3109)
                     /locus_tag="VF222_11220"
     CDS             complement(935..3109)
                     /locus_tag="VF222_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7143036.1"
                     /translation="MRTTLYFFLGVIITIVIGNSVIFFAESDSKIIFSNWILIINSLT
                     AAGLSAMILLKDKDSDEGGDKTNILLTLGLIFWFIANIIWAYYEVVLDIVSPVPSLAD
                     LFLLSAYGFLIFRLISIYRKLGHITNKKILILIISGTGLFLIYILNLTLDLTELSNFR
                     GFMLFIVTIAYPTLNSILTALALMILLRIKKEKQHFVPWVCELVGFLAIVVGDSWFAI
                     IVLTAFVEQLWMSALLLSAHYLLIAAGLIWYLRYYIKWQSKDLIYKIIINTRRNKTFK
                     KILFSSTISISFLIFAGFFYTSVYSGDQSMDFFIKKSSSYPQLSSTYKEDTRKDFVVG
                     AILPYTGSFSSIGKSVKVALEKAEYDVNKHFEGMNSSSQFNLLMANSKSSPEDSLEAI
                     KQLHSFGAKIIVGPAFGATVNATKEYADANNITLISYSSTSPLLSIEGDNLFRLVPDD
                     TNQGKIVAERMIKDGIKVIVPIWRGGIYGNELYKSTKSNFENLGGEVEEGINYKPHTG
                     KFATSLHRINFLMWNQELERLNNIVGEAIKKYGTNSVGVYVISFDEITPILIQSTLYE
                     NLGKVRWYGSDKIAQNHQITKNVDSALFAMKTNLSNPLYSIDSESKKLHDLEEVLEKQ
                     LHEVSSITYPALAYDSYWIAALSLDKNNTMFNNSNENLTKSFKEIILETVDSFEDGIS
                     GKIKLNKAGDRIGGNYDFWIVAKDNHTQNYEWEKEHNYRTVT"
     gene            complement(3430..3672)
                     /locus_tag="VF222_11225"
     CDS             complement(3430..3672)
                     /locus_tag="VF222_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143037.1"
                     /translation="MCQKTIYTHLCFPIFYVLIYSGEVKTCTIENYIFAADFFPPQPD
                     DNAADNTQSSLSQQSNNQAIQPQPNTLRDIISDRTN"
BASE COUNT         1327 a          532 c          537 g         1411 t
ORIGIN      
        1 aaatgaaaga tttgttgcgg ttcgatttaa ttgacgttca aatggttcgt caaaatatac
       61 atctccatta tttaataaaa atacaactga gaaaagttca ttaaattgct ccaacagtat
      121 tttacctatt tttctctttt ctaaatctgc atcttctggt atacccttaa cttttggatc
      181 tatcaaattt acatcaggag gttgtaaaat ttgtggtaga ctacctgcaa tttgtaaagc
      241 cgtagcaagt tttgttacct tactttcaag tatatttttt atattttcca gttcatttaa
      301 aagtgaagaa ttgttatttt cgtttgtgtt attattatag tattgagtat cttgagcata
      361 aatagagata aaaggtgagt ttgcggaaaa taagataata ctagtaataa taataccaaa
      421 taaaaaactt gaaatcttta acattatcta ttcttgagaa ttttaagaat aattcaatat
      481 aattctatcc ataacttgat aatatagcag ttgatatata ctatcctata tagaaagagg
      541 atattactga aaacatttgg tatagtaata aggttcggag ggcttcgatt tccaattata
      601 acagggttcg gtaacataat agataaagtt tttgtttttg gaagtatatc tattatctct
      661 actagttact ttatagataa ttttgaaaac tttcttttgc caatcaatta taggaaagca
      721 ggaggtgtgt gaattgtttt tgacataata tttttggcac atcttataag acaataacaa
      781 gacttaacat gaacttaaca atatttcttc tgggattcta ataatttctt tttctatgtt
      841 attatgaaaa tttttactaa ttaatgttta atttgaaaat tattaggata ttgtatctca
      901 aaataagatc agaaattttt tattatcatt taaatcaagt tactgttcta taattatgct
      961 ctttctccca ttcataattt tgagtatggt tatctttagc aacaatccaa aaatcataat
     1021 ttccaccaat gcgatctcct gctttgttaa gttttatttt tcctgatatt ccatcctcaa
     1081 atgaatcgac agtttctaga ataatctctt taaatgattt ggttaaattt tcattactat
     1141 tgttaaacat agtattattt ttatcaaggc ttagtgcggc tatccaataa gaatcataag
     1201 ctagtgcagg atatgttatt gaacttactt catgcaattg tttttctaat acttcttcca
     1261 gatcatgtaa tttctttgat tcgctatcta ttgaatataa aggattagaa agatttgttt
     1321 tcattgcaaa caatgccgaa tcaacattct ttgttatctg atgattttgt gctattttat
     1381 cactgccata ccatcttact tttcctaggt tttcataaag ggttgattgg attaaaatag
     1441 gtgttatctc gtcaaaagat attacatata cacctacaga attagtacca tattttttta
     1501 ttgcctcgcc tacaatattg tttaatcttt ctaattcttg attccacatt aggaaattta
     1561 ttctatgtaa actagtggca aatttgcctg tatgaggttt ataattaata ccttcttcta
     1621 cttctcctcc tagattttca aaattagatt tagtagattt atagagctca tttccatata
     1681 taccacctct ccagatagga acgataactt ttataccgtc cttgatcatt ctttctgcaa
     1741 ctatttttcc ttggtttgta tcatcaggaa ctaaccgaaa aagattgtct ccttcaatag
     1801 aaagtaatgg cgaagtactg gagtaactaa taagggtaat attattggca tctgcatatt
     1861 ctttggtagc attaactgta gcaccgaatg caggtcctac aataatttta gctccaaacg
     1921 aatgtaattg ttttattgct tcaagagaat cttcggggct tgattttgaa tttgccatta
     1981 atagattaaa ttgtgatgaa gaattcattc cttcaaaatg tttgtttaca tcatattcgg
     2041 ctttttctaa tgctaccttg acagatttac caattgagga aaaggaacca gtatatggta
     2101 aaatagcgcc aacaacaaaa tcctttctag tatcctcctt atacgttgat gagagttgag
     2161 gataagaaga actttttttt ataaaaaaat ccatactttg gtcaccgcta taaacacttg
     2221 tataaaaaaa accagcaaat attagaaaag atattgatat agtggaacta aacagaatct
     2281 ttttgaaagt tttatttctt cttgtattta ttataatctt gtaaatgaga tcctttgact
     2341 gccatttgat ataatatcta agataccata taagaccagc ggcaattaat aaataatgtg
     2401 cagacaataa taatgcagac atccataact gttcaacaaa tgcggttaga acaataattg
     2461 caaaccaact atctccaaca actattgcta aaaatcctac cagttcgcag acccagggaa
     2521 caaagtgttg tttttccttc tttatcctga ggaggatcat caatgcaaga gctgttagaa
     2581 ttgaatttaa tgtaggatat gctatagtga caataaaaag cataaaacct ctgaaatttg
     2641 ataattcggt aagatctaga gtcaagttta atatgtatat caaaaataaa cctgtaccag
     2701 agattattaa aattagaatt tttttatttg taatgtgacc aagttttcta tatatactta
     2761 tcaatctaaa tatgagaaat ccataggcag acaataagaa taaatctgct aatgaaggta
     2821 ctggagatac aatatcaagc actacttcat aatatgccca tattatatta gctataaacc
     2881 agaaaattag accaagtgtt agaagaatat tagttttatc tcctccttca tcactatctt
     2941 tatccttaag gagaatcatt gcagaaagcc ctgcagcagt aagagaattt attatcaata
     3001 tccaatttga aaagattatt ttactgtctg actcagcaaa aaaaataaca gaatttccaa
     3061 tgactattgt tataattact cctaaaaaaa aatatagagt agttctcaat tatgcttctt
     3121 tattataatt attgatatca gttataaata cctataaata tcgaaagaaa ttaattaata
     3181 tattccttat tatatataca catttaaaat ttgatagcta aaaatatggc ataatctact
     3241 acttaatata atataggtta tatcatatta tctatttttc tcatactcct taaattttag
     3301 gcaaataatt gtatatgaat agacaaattg atcattaaac ttgagtaaaa gatattttaa
     3361 ttgtatataa atatagaaat catcattagg aatatttatt gttcaaagaa aaataatatt
     3421 ttgtttttgt tagtttgttc gatcagagat tatatctctt aaagtattgg gttgaggctg
     3481 tatggcttga ttgttggatt gttgcgataa tgatgattgt gtattatctg cagcattatc
     3541 atcaggttgg ggagggaaaa agtctgctgc aaaaatatag ttctctattg tgcatgtctt
     3601 tacttcacca gaatatataa ggacataaaa tataggaaag cagaggtgtg tgtaaattgt
     3661 tttttgacac aattttgtag ttcaaaaatg cattttaaaa ctaagattta tacagaaaaa
     3721 ttattcttaa tatgggatta aatatttatc agtgagagta tttaaaattt aaaaatatta
     3781 taataaaaat aatatttaac tttaagt
//
LOCUS       DASVEV010000256         7890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_717167, whole genome shotgun sequence.
ACCESSION   DASVEV010000256 DASVEV010000000
VERSION     DASVEV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717167"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(871..1188)
                     /locus_tag="VF222_11230"
     CDS             complement(871..1188)
                     /locus_tag="VF222_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143038.1"
                     /translation="MSYGGYEIDAGENHGISNRQLALIFGISIVGIFIVFALFIFPIQ
                     NLFPESITEQVTIISKSDGECVVNSRDHPRGISNCNYDISDKLQITYKYGTAPIETHK
                     KIN"
     gene            complement(1545..2033)
                     /locus_tag="VF222_11235"
     CDS             complement(1545..2033)
                     /locus_tag="VF222_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143039.1"
                     /translation="MTSRLKFCSNCGKTLDLNKEIKKYAKCGIDLQQQLERSTYNKSI
                     VDQLPYKSPGTAALIAFIGGIFALLGLGHIYVGKVERGIGILISGIIIYVLLIIMVIS
                     FPSFLILAPDESSLGIGVILMVFVLGIAYFVLWIWQIFNARKLAKRFNESVRTTGGKE
                     PW"
     gene            complement(2128..2280)
                     /locus_tag="VF222_11240"
     CDS             complement(2128..2280)
                     /locus_tag="VF222_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143040.1"
                     /translation="MASASSRTDTKSTPSKIPCEICGLLFDTVDIKEEHKKLEHVEHK
                     PPSGVT"
     gene            complement(3639..4004)
                     /locus_tag="VF222_11245"
     CDS             complement(3639..4004)
                     /locus_tag="VF222_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143041.1"
                     /translation="MNNNGANFQMSTKSTLIEDNNKESVNKDKEKAKSFTDKIVNKIS
                     HIGDIRGIEIAVDQSSNVKFITFGLFVLIGFIVLIYAILVAVVADRVIQSANVFFVVT
                     IISISTLFGTLVYQIRQSK"
     gene            4420..4581
                     /locus_tag="VF222_11250"
     CDS             4420..4581
                     /locus_tag="VF222_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143042.1"
                     /translation="MRHPITLAKNILLVDDEPDVIYAIKNILEDNGLQINSFNDPITA
                     LKSYRTINL"
     gene            complement(4696..5328)
                     /locus_tag="VF222_11255"
     CDS             complement(4696..5328)
                     /locus_tag="VF222_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7143043.1"
                     /translation="MVSKNKLYYYYYLVIDLESSSSQHIPLDIQIEKLEKLRLCVNKI
                     LNSNLERLHQKKVRYINSTGDGYFIIFDIGEDALKFSIDLHKYLNLHNRKIRNNKIYS
                     SNEIDSKTITVNTGISCGISKPIKQIDGQIAYWGSDVILAHRIVEFAKGGYILCSSDV
                     YKNMKNINFGEFIDLKCSTNFKHNLKTDMYLFYNKEDKEKFENINIHLLR"
     gene            complement(6368..6991)
                     /locus_tag="VF222_11260"
     CDS             complement(6368..6991)
                     /locus_tag="VF222_11260"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7143044.1"
                     /translation="MNKKIPLIVGILIFDDVEILDVAGPFEVFAVTRLNEKQRLQQSS
                     PFKVYLISETKKQIIAIGGLRLTPDVTIGKCPELDLLIIPGGWGTRKESKNKILLNWI
                     GNQFTNDRLIASVCTGSSLLGKAGLLDGRDATTHWRAFDFLQESAPKARILKDVRFTL
                     TEPIFTSAGISAGIDLSLRIVSHFFGIEVGQATARHMEYPYPDSDRR"
     gene            7592..7714
                     /locus_tag="VF222_11265"
     CDS             7592..7714
                     /locus_tag="VF222_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143045.1"
                     /translation="MGNIESCRREERGMDMKEEPLIDFVGKIDEDILTRKFSNH"
BASE COUNT         2881 a         1151 c         1003 g         2855 t
ORIGIN      
        1 cttccaattc aatagtaatc agtaattgct tatcgtctag ttctttaaca ttatctaaat
       61 aatttttagg aatccaaatc acacgattat ctttcattcg gtttaacttg ccagcaaatt
      121 taatcttgat tttgtctccc aatatcggaa ttaggctttc catattattt atactatagt
      181 ctaccttata aaaggcttac tatagtatac attaattggc tatagttaat ctcctcttgc
      241 tacaggatgg aagtacaatt agtacctact acaagtccag gtgtaggaat atatactgtg
      301 tatgcaattt gctttgacaa ttcaccataa ccatttttta tttttttaat actcaaatat
      361 atcatacaaa tgcaaatcgt attttgtcat attgtaaatc aactatctat aagccgaatc
      421 tcattgttgt tcaggctatg acaatttatt aacattagtc gattaactaa gttcaccgat
      481 ttttaaatat actgatatgt aaactataat acattattcg ttaacaacaa gatggtcctt
      541 agaatagcca tagtcaaaaa tcaatgcctg atctgtattg gtccatccag gctatggcta
      601 ttaaatatta attaaactct ctccaactat aaaatactat aatctagatt gattatacat
      661 cttatatgca aatcataaga tttcatcaaa gccatagact tctcttatcg aactactcct
      721 ctcagcaaat agtagtgggt tatggcttta ttattctctt tagtattata cctaaatatg
      781 atagtttaat tgagcactaa aacatattag tggtatctta taagcatact ataagataga
      841 tatcattttc aaaattaaaa atgtctaatt ttaatttatt ttcttatggg tttcgattgg
      901 agcagttcca tacttgtatg taatctgcaa tttatcgctt atatcatagt tacagttcga
      961 aattcctcta ggatgatctc gactgttaac aacacattcc ccatcacttt tactgattat
     1021 tgtgacttgt tcggtaattg attcagggaa tagattttgt atcggaaaaa taaataaagc
     1081 aaatactata aaaattccta ctatactaat tccgaatatc aacgctagtt gtctgtttga
     1141 gatcccatga ttttctcccg catctatctc atatccacca taactcataa tagcatttaa
     1201 tttaattatc ctataaagta tattataaat tatatagata gtgcctaaca aaattaagta
     1261 ctacaattga ataaatttaa aagaagactt gacatttata agaaatcttt ttgaactaca
     1321 aattgtatca aaatcacttg acatacctct gagtgagcgg tcacgaatat gcttatattt
     1381 taatgtaaaa ctaactttat aagaaataaa atttaaccgt taaaaatatt tataattttc
     1441 tcaattattt attaaactta atgtttttct aatgctgctt cctgttaata atttaaccta
     1501 gttattagat ttgcaagaaa tagaaaaaga tctggtttaa attttcacca tggttctttt
     1561 ccaccagttg ttcgtactga ttcattaaat ctcttcgcta gttttcttgc attaaatatt
     1621 tgccatatcc ataatacaaa atatgcaata cctaagacaa acaccataag tattactcca
     1681 atgcctagag atgactcatc gggtgcgaga attaaaaaac ttggaaaaga gattaccata
     1741 ataataagca gaacataaat gatgatacct gaaattaata tcccaatccc tctttcaacc
     1801 tttccaacat atatatggcc tagaccgagt agagcaaata ttcctccgat aaatgctatg
     1861 agtgctgctg tcccaggact tttgtagggt agttggtcta caattgattt attatatgta
     1921 gatctttcta gttgttgttg aaggtcaatt ccacacttag cgtatttttt aatttcttta
     1981 tttaaatcta atgtcttacc acaattgctg cagaatttta gtctactagt cattcatata
     2041 caaatagatt accggctaat aaagaaatct taaaggaaat taggaaaaaa ataataatta
     2101 attctttttt aaattctttt aatcagatta agttactcca ctaggtggtt tgtgttcaac
     2161 atgttctaat tttttgtgct cttcctttat atctacagta tcaaataaaa gtccacatat
     2221 ttcacagggg attttagaag gtgtgctctt ggtatcagtg cgggatgatg cagatgccac
     2281 tattacaata acaacttcaa agttaataat actttagatt aaataataat aataatatta
     2341 atcgatgata ttagacaaaa taagatatta ataaaattta acgctaaatt atattctttc
     2401 attaattata taattacaat actaatcaat gattgataca atgagatata ttctatatct
     2461 cctttaatca atagtggtat tatcatcatc gtcgtcgtat tcagaactta gagaagatat
     2521 taataatata ataaaagaat ggcattttgg aacagaagat gtgtttgagg ataagctaat
     2581 tctgtattaa atgttccata tagtaaaggt aactacaatt cgatatcttg aaattcattt
     2641 gataatgtta ttttattatc atttgtatct tttgcacaaa taaaaaccat attttttata
     2701 tcctccatat gacacgtagc cgttggctat gacaatttat taactaaaca attagatatg
     2761 gatccgtgaa tcacgcgttt aggcccaatt ttctagagaa acgattttgt gacccaaaga
     2821 gtaatagata aaattaacag cttattccaa aagccggatg tactcttgga tagaccgatg
     2881 ctattattta gtaaatataa tgattcacta tgagtattat tgaatagcat taatccataa
     2941 aaaacccatt gattatcgat caatcgtcct aggtcggaag atatccaacc gatattttgt
     3001 ccatcggtag tctcaatcgt tccatgtcct ctcccaagaa gcagttcatc agagaagtaa
     3061 gtatttaaaa tgtctggttg ttagttacat tccctacatt atttaggaac ccgacttcaa
     3121 aatattttcc tctgtgattc gataaggggt caatgatata tttttggaac ctaaaaactc
     3181 acttgtatta gcattatata ccgcatcggt tacgtttaaa gacaatgtat tttgatcttt
     3241 aatattcaac tgaggaaact ctgctagtat atctgtaaca gaatgaagtg gaaaaagacc
     3301 tactattata gtggagatat tctgtttgaa tattataaat cataagagaa atacaaattg
     3361 gtatttttag ttttataatt tcatatatat agcaaaacta tgattaaata tatattataa
     3421 atattaatgt aaaaaataac agttttttaa agaattaggc tttggaaaat gttcaaaaat
     3481 tgcaaactac tggaatacaa ctaattttta tttaagggtt ataaatcaga gaaatgtaat
     3541 aattacataa attagatata ttatgaggca ctaaaatgat acaaaaaagg ctatgagaaa
     3601 taatgcttaa tttaggatat acttaagtct ataatgcact attttgattg acgaatttga
     3661 tatacaagag ttccaaataa ggtagaaatg cttattatag taactacaaa aaatacattt
     3721 gcggattgaa ttactctgtc tgctactact gcaactaata tagcatagat taatactata
     3781 aaaccaatta aaacaaataa accaaaagtt ataaatttta catttgatga ttgatctact
     3841 gcaatttcaa ttcctctgat atcacctata tgtgatattt tatttactat tttatctgta
     3901 aaggatttag ctttttcttt atctttgtta actgattctt tgttattgtc ctctattaac
     3961 gtagatttag ttgacatttg gaaattcgcg ccattgttat tcataattat attttaacat
     4021 tatagcttat tataattata atcatgtagt aaaaatcaaa taaatagcat aataaatatc
     4081 aaaaacatga agacatccaa aaagaattca cctaatacta agaattctct aaaacataga
     4141 caatgaatga gacttcaaat cccattgcct cgattgaatc gttgattaga taaattctaa
     4201 accagaaata aatagaaata actctaaaac tttcaaaaac ttattaatta tgttaccgag
     4261 tccttttata atgcgaattc gatattctta tctaaaacat atagttaata aaagaagatt
     4321 ttgaagaaaa gatagggcaa aaaataattt gaatggtaga gaatatactt tttattttat
     4381 gctttcgata attaatagtg agaggtgtaa ttatttataa tgaggcatcc aataacattg
     4441 gctaaaaata tcttattagt tgatgatgaa ccagatgtca tttatgcaat aaagaatatt
     4501 ttagaagaca atggattaca aatcaactca tttaatgatc ctattacagc tttaaaatct
     4561 tatagaacaa taaatttata attacaaaat tgtgtcaaaa tcaattgatc atctgaagaa
     4621 cccagttgtc aataaaattc ttaattatct ccttttagtg ttcttttgtt aaaagttatc
     4681 tgaaaaaata ctaattcacc ttagaagatg aatattgata ttctcaaact tttctttatc
     4741 ctctttatta taaaataaat acatatctgt ttttaaatta tgtttaaaat ttgttgaaca
     4801 tttaagatcg ataaattcac caaaattaat atttttcata tttttgtaga catcagaact
     4861 acacaatata taacctcctt tagcaaattc aacaattcta tgggctaata taacatccga
     4921 tccccaataa gctatttgtc catctatttg tttaataggt ttactaattc cacatgaaat
     4981 cccagtatta actgtaattg tttttgaatc aatttcgttt gagctataaa ttttattatt
     5041 tctaatcttt ctattgtgta aatttagata tttgtgaaga tcaattgaaa attttaaagc
     5101 atcttcccct atatcaaaaa taataaaata accatctcct gttgagttta tatatcttac
     5161 tttcttttga tgtagtctct ccaagttaga gtttaaaatt ttattaacac aaagtcttaa
     5221 tttttctaat ttctcaattt gaatatctaa tggaatatgt tgagatgaag aactttctaa
     5281 atcaataact agataataat aatagtataa tttatttttg gaaaccatat tgagatacaa
     5341 atacaaagta agagtatatt tttcttatta tcgtattaaa attatatcat tcctactaat
     5401 actttaagtt aactcattta atcaaaattc tattagataa agcaatatat tatattttca
     5461 gtcacagttt tgaagtaaaa cactatttca ctaattatta aatattattt ttataaccat
     5521 tttctaaatt gatgtcttga taaatagctc atttattata gtattctacc cccatataat
     5581 atattcatta ttaggaaaaa actaagaatt ttggtttatt tctaattaat aaagaattgt
     5641 gtttcttatc aaatttttac cattcaaaat gattaaatta tatttaatct ttatttgaaa
     5701 tgaatttcat taataataaa tttaggaact gatgacctcg tattaccttc tgctaattct
     5761 attataataa cagagagaat acctggcgca tggttagtcc aattcaaaga aggtggtcat
     5821 gcactaatgt ttcaataccc tgaaggatta agttctgttg taaatacatt tcttgaaaat
     5881 taatttttaa tatttttatt ttttattatg atgttaaaaa ggacatagat attcattaca
     5941 tattttagat tgattaattc aattacaaaa ttgtgtcaaa aataggagaa tatcctccga
     6001 ttgaacgcta acaaaaaaaa ttttaataac acaaaaccta aattcaaagt aaaatttatt
     6061 ctacataaaa tatactatgc tactaatccg gagaatgggt attaacacct tgcatgaaca
     6121 ttttccgaat agtgtgactt atccaaaata acttttggat tttatttatt atagtttgtt
     6181 gtaaattatt ctctatggtt attctataac aaataaaatg ataataaatc tttctgatcc
     6241 taaaaactag taatactttt tgtttttaac tataacattg tgccaaaatc agatgatctc
     6301 attctgttga gtgatagatt tagttcattc ccaagctcat catagattaa caatatttat
     6361 tagtatttta ccttcggtca ctatcaggat aaggatattc catgtgacga gctgtagcct
     6421 gtcctacttc aattccaaaa aagtgactta caatacgaag agataaatct attcccgcgg
     6481 aaatgcctgc agatgtaaat attggttctg tcaaggtaaa tctgacatct ttgagaattc
     6541 gtgcttttgg tgcagattct tgcaaaaaat caaaagctcg ccaatgagta gttgcatctc
     6601 ttccatctaa caagcctgct tttccaagaa gactagatcc ggtacaaact gaggcaatca
     6661 atctatcatt agtaaattga ttaccaatcc aatttaatag aattttattt tttgattctt
     6721 tacgcgtacc ccatccacca ggtattatca ataaatccaa ttctggacat ttaccaattg
     6781 tcacatcagg agttaaacgt agaccaccaa tagctataat ttgttttttt gtttcagata
     6841 ttaaatatac tttaaatggt gatgattgct gtagtcgttg cttctcattt aaacgagtta
     6901 cagcaaatac ctcaaaaggc ccagcaacat ctaagatctc tacatcatca aaaataagaa
     6961 ttccaacaat taatggaatt tttttattca atttaaagta tataacaaaa tatctctttt
     7021 tttaaatttc ctttagaatt ttaaaaacat ggatattgag attattgttg tatatttttc
     7081 cattatttta gtagcatctt cataggtaaa ggcttttagg gttacagcga atattcttgt
     7141 atgatcagtt tgtttattgt taatatattt tgtagggtta tattatacta atgacatcga
     7201 gtgaaactca gatgtaaaaa tgtggtatca tcttcaatac tgtagaggat ctgtatgaac
     7261 atcaaaaatt agaagaagaa gataaaatgt taagacataa aagattttca gatggtaaat
     7321 gtgtataatc ctttaagtat taataattat taatattaac caatttgtat ttttcagtga
     7381 gacaactttt gaatctgaag cagacagaaa gattgaatat cataatttat aggaagaatc
     7441 tgaacatcta aaaaaacata atgataatta atttacttaa taataataat atttaaatta
     7501 tattaccagg attcagcagc aactagacct aaaacctcga acaagcgtat gttaaacaac
     7561 attacattaa attcacaaaa agtattcagc aatgggaaat atagaatcat gcagaagaga
     7621 agaaagagga atggatatga aagaggaacc attaattgac tttgttggta aaattgatga
     7681 ggatattcta acaagaaagt tttccaacca ttagaaaaca aaaataaaaa attcttcatt
     7741 aatctagata attttactat gaagatatca atctggtgtt ccaccaatac aagcacagta
     7801 accaaattca tctattctag tattacaaaa attgcaatac ccaaatagat tataattcac
     7861 atcactagca tattttccaa aattcttaaa
//
LOCUS       DASVEV010000257         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1104827, whole genome shotgun sequence.
ACCESSION   DASVEV010000257 DASVEV010000000
VERSION     DASVEV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104827"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VF222_11270"
     CDS             complement(<1..728)
                     /locus_tag="VF222_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX7143046.1"
                     /translation="MGVSEIDKTKNKSVFKIKKRDGRVVNFDESKISQAIYKALIATG
                     KPNFQLAEELVDKVVKKMTLNSTSFDKSFIPSVEDVQDIVESILIEDGLSDTAKAYIL
                     YRHERRKIREDKIKILNKKNLDEIDKDFDINSLRVLSSRYLLRNENNEIIEAPKQMFE
                     RVALLVAISDLLHDPLIFHLPGGYDQNTVEATSYYDKISDFDLKLKIGSYYLNKYHFE
                     SLIRLYTQLASKGQMKTSFKDILK"
     gene            complement(940..3075)
                     /gene="nrdD"
                     /locus_tag="VF222_11275"
     CDS             complement(940..3075)
                     /gene="nrdD"
                     /locus_tag="VF222_11275"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX7143047.1"
                     /translation="MERVQSQIVNKTGSKRGSILESASRRVRMIFSVMASPNRIDILR
                     ILYAKGPLTYSELKSLAGFKSKKESGKFAYHLRKLLRQSLVSLNKAERRYTITNLGKL
                     VLSLARQIEERSIIESGKLYVRTTKPSIEEFDSNKIIQSLVREANMPLELAHKITEEV
                     ENKIYKLQTSYLTSSLIREMTNSILIEHGHDDYRNKLARLGLPPSDMVSLIHETSTHN
                     LEIPDLVVKTSQSIFTEYLLNSSLPKDIVDMHLTGEINIGKSGFWNIIPDAIFINLNS
                     IIDIFEDIKGRYLTISRIIKSNTFQTPESIVSIIFSLLSREASREIVIEGFLDFLQKK
                     SEKGSLVKENIANIFFLTSTISSYGSFSPNITLSIKLTNYERSNVNSLLEGYEKYITI
                     TPIPTIALSIVYDDLTDLDPFIDKLIQLSKAGGIISFSKYKVRARYGLSKSEEMPNTS
                     TVVILQSLSINLPRIAYQSNKDETYFRARLAILLKPLLSIIAQRFLSISDLIQKGTIP
                     IVSQTTSNLKLGKIEAMVNLVGIQESVYEILGYTPENNGDNVIKKVIQTAVDIINENT
                     QESQKNAEIRITMLKDESAFRFKDLDIEKYGKSLTINNDKNIIYSQGLIIDGEKIVSN
                     SLDLDPIIEYYSSIEDLLQGGVSIDLDITNVSNIEDVKKVIKSSTNLPFFFLAQNFSI
                     CTICGERSIISGLKICEKCESCKISSISS"
     gene            complement(3168..3785)
                     /locus_tag="VF222_11280"
     CDS             complement(3168..3785)
                     /locus_tag="VF222_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143048.1"
                     /translation="MQKYPQLQSKLSNASNVVNNQLAKLRILESRFNTLDAELHNKVI
                     TNIKNGDNVRAKALANELVNIRKIKHTTQKLLMSLEVVVIRFSTISEFADILDTINPM
                     IETVKEVKNDIIRTVPAATSIISEMSTLTSDILINSNVNVNIDRLSIPVNSDALEILN
                     EVQNIMEEEAKSKLPDIPNNISHKTKYTSKQNELSTQNSNILLEA"
     gene            complement(3898..4284)
                     /locus_tag="VF222_11285"
     CDS             complement(3898..4284)
                     /locus_tag="VF222_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX7143049.1"
                     /translation="MNNSNSTAVREKFIESAQNSKILTRKCADCSTIRLATVLYCENC
                     HGKKFKLIDYPGIGYVNTFTIHTVPPEGFDDVDGYAWVVFTLEDASIRASGFLPGIKK
                     PAELPIGSKVKVNGFDEKHGLVLKKL"
     gene            complement(4291..>4692)
                     /locus_tag="VF222_11290"
     CDS             complement(4291..>4692)
                     /locus_tag="VF222_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX7143050.1"
                     /translation="STIPGTVIAANQAYKMAGITAKDVDFAEVHDCFTIAEIIDVEDL
                     GFFQKGKAAREIREGSTSRKGKIPINPSGGLKSKGHPIGATGIGQIVEVFEQFSGLAG
                     ERTIKGAEIALTHNFGATGASAAVHIFKKVN"
BASE COUNT         1522 a          748 c          704 g         1718 t
ORIGIN      
        1 aattttaaaa tatctttaaa cgacgttttc atttgtccct tagaagctaa ctgagtgtac
       61 aatcgaatta atgattcaaa atgatatttg tttaagtaat aagatcctat ctttagttta
      121 aggtcaaagt cacttatttt atcataatat gaagtggctt ctacagtatt ttgatcgtat
      181 ccaccgggta aatgaaagat aagtggatca tgtaagagat cagaaatagc aacaagaaga
      241 gctacacgtt caaacatctg tttaggagct tcaatgattt cattattctc atttctcaaa
      301 agataacgtg aggataagac gcgtagggaa tttatatcaa aatctttatc aatctcatca
      361 aggtttttct tatttaggat ttttatttta tcttctctga ttttccttct ttcgtgtcta
      421 taaagaatgt aagcttttgc agtatcagat aatccatctt caataagaat tgactcaact
      481 atatcttgaa catcttcaac acttggaatg aaagatttat caaatgaagt gctattgaga
      541 gtcatttttt tgacaacttt atcaacaagt tcttcggcta attgaaaatt aggtttgcct
      601 gttgcaatca aagctttata aattgcttgt gatatctttg attcatcaaa atttactact
      661 ctaccatctc gtttttttat tttaaatact gatttatttt tagttttatc tatttctgat
      721 actcccatac aattcgccgt ccaatttcaa tttatatctt aaatgatata acacttaaat
      781 tttattatta tgcaacttaa ttagcgaaaa attctattat taaatgagtt attcaaaata
      841 tgttacagaa tatacctatt agtataaaag gaaataagtt gtaaaattgt tttgatagtg
      901 ttaatataat gtgactgatt agaaagaata acagaaaagc taggaagaga ttgaagaaat
      961 tttacaagac tcacattttt cacaaatttt taaaccagag ataatagaac gttctccaca
     1021 aatagtacat atcgagaaat tttgagcaag aaagaaaaat ggaagattag tagaagattt
     1081 aataaccttt tttacatcct caatgttact aacattagta atatccagat caatagaaac
     1141 tcctccttgg agaagatcct caatagatga ataatattct attatcggat ccaaatcaag
     1201 agaatttgaa acgatttttt cgccatcaat aattaatcct tgagaataaa ttatattttt
     1261 atcattatta attgttaatg attttccata tttctcaata tccaaatctt tgaatctaaa
     1321 cgcactctcg tcttttagca ttgttatcct aatctctgca tttttttgac tttcttgggt
     1381 attctcattg attatatcaa cagcagtttg aatgaccttt tttatcacat tatcaccgtt
     1441 gttttcggga gtatatccta atatttcgta tacagattct tgaatcccca ctagattaac
     1501 catagcttca atttttccca atttcaaatt ggatgtcgtt tgacttacta taggaattgt
     1561 tcccttttga ataagatctg agatggagag aaaacgctgc gcaattatag aaagcagtgg
     1621 tttaagtaat attgctagtc ttgctctgaa atatgtttca tctttatttg attgatatgc
     1681 tattcttgga agattaattg ataacgattg taatattact actgtactag tatttggcat
     1741 ttcttcagat ttagataaac catatcttgc acggacttta tattttgaaa atgagattat
     1801 ccctccagct ttggaaagtt gtataagttt atcaataaaa ggatcaagat cagtaagatc
     1861 atcataaaca atcgagagag cgattgtagg aatcggtgta atagtgatat atttttcata
     1921 tccctccaag agtgaattaa cattagatct ctcatagtta gtcaatttaa tagataatgt
     1981 aatatttgga gaaaatgatc catacgatga gattgtagag gttaaaaaga aaatgtttgc
     2041 tatgttctct ttcacgaggc tacctttttc gcttttcttt tgaagaaaat ctaaaaatcc
     2101 ttcaataaca atttctcttg aggcttctcg gcttaataag gagaaaatta ttgagacaat
     2161 agattctggt gtttggaaag tattagattt aataatccta gatatagtta gatatcgtcc
     2221 cttaatatct tcaaaaatat caattataga attaagatta ataaatatag catctggaat
     2281 aatattccag aatccacttt tacctatatt gatttcacca gtcaagtgca tatcaacaat
     2341 atccttaggg agagagctgt ttaaaagata ttcagtaaaa attgattgtg aggttttaac
     2401 aacaagatct ggaatctcca gattatgagt actagtttca tgaataagag acaccatatc
     2461 agatggagga agtcctaatc tagcgagttt atttctataa tcatcatgtc catgctcgat
     2521 taaaatggaa ttggtcattt cccttattag ggaagatgta agataagagg tttgaagttt
     2581 ataaatttta ttttcaacct cttctgtaat tttatgtgca agttctaatg gcatattagc
     2641 ttctcggact aaagattgga ttattttatt agaatcgaat tcttcaattg agggttttgt
     2701 agtccttaca tataatttac cactttcaat aatagatctt tcttcaattt gacgtgctag
     2761 acttaatact aatttaccta gattagtaat tgtatatcgt ctttctgctt tattaagaga
     2821 gaccaacgat tgacgtaata attttcgcaa atgataagca aacttgcctg attctttttt
     2881 cgatttaaac cctgcaagag attttaattc agaataagtc aaaggacctt tagcataaag
     2941 aattctaagt atatctatac gatttgggct agccataaca gaaaatatca ttctcaccct
     3001 tcgcgaggcg gattccagaa tacttccacg tttggatcct gtcttattaa ctatttgtga
     3061 ttgtactcgc tccattgcac cggttatgaa taacaggtaa ttaaaactac tttgtaaagt
     3121 tttttataca attgatgtaa tggatactaa tcagactact cattgaacta agcttctaat
     3181 aatatgttgg aattttgagt tgagagttca ttttgcttcg aagtatattt agttttatgg
     3241 ctaatgttat taggtatatc tggaagcttt gactttgctt cttcttccat gatattttgt
     3301 acttcattaa gaatttcgag tgcatcagaa ttaacaggaa tagagagacg atctatattc
     3361 acattcacat tagaatttat caatatatct gatgttaatg ttgacatctc tgaaataata
     3421 ctagtagcag cgggaaccgt tctaattata tcattcttaa cttccttaac tgtttcgatc
     3481 attggattaa tagtatctaa aatgtcagca aattctgata ttgtagaaaa tcttattact
     3541 acgacttcta aactcattaa tagtttttga gttgtatgtt taatttttct aatattcact
     3601 aattcgtttg ccaatgcttt agcacgtacg ttatcaccat tttttatgtt tgtaattact
     3661 ttattgtgta actcagcatc taaggtatta aatctgcttt ccaatatcct cagcttggca
     3721 agttgattat taactacatt acttgcatta gagagctttg attgtaactg aggatacttt
     3781 tgcatatgtt ttttaatata gtaatagaat gttaaggtta gagttaagct aactagacca
     3841 attgatttca taaagtttag ttacttatta taacacaatt agaaataatt taagagatca
     3901 aagttttttc aaaactaacc catgtttctc atcaaaccca ttaactttaa cttttgatcc
     3961 aataggaagc tctgccggtt tttttatacc aggtaaaaat cccgaagccc gaattgatgc
     4021 gtcttctaga gtgaacacaa cccaagcata accatctaca tcatcaaatc cttctggagg
     4081 cactgtatga atagtaaagg tattcacata acctatgcca ggatagtcta ttaatttaaa
     4141 ttttttaccg tgacagtttt cacaatataa cactgttgcc agccttatag tgctacaatc
     4201 agcacacttc cttgttaata tcttggagtt ctgagcggat tcaataaatt tttctcttac
     4261 tgctgtactg ttagaattat tcaatgctga ttaattcacc tttttaaata tgtgaacggc
     4321 ggcgctagca ccagtcgcac caaaattatg agttaaagca atttctgcac ctttaattgt
     4381 tctttctcca gctagtccag agaattgttc aaaaacttca acaatttgac ctatgccggt
     4441 agctcctata ggatgacctt ttgactttaa tcctcctgat ggatttattg gaatctttcc
     4501 ttttctggaa gtagagcctt ctctaatttc tctagctgct ttacctttct ggaaaaaacc
     4561 gagatcttct acatctatta tctctgcaat tgtaaaacaa tcatgaactt cagcaaagtc
     4621 cacatctttg gcagttatcc cagccatttt atatgcttga ttagcagcaa taactgtacc
     4681 aggaatagtt ga
//
LOCUS       DASVEV010000258        16169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1689250, whole genome shotgun sequence.
ACCESSION   DASVEV010000258 DASVEV010000000
VERSION     DASVEV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689250"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF222_11295"
     CDS             <1..382
                     /locus_tag="VF222_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143051.1"
                     /translation="NLIKNKFPQPVKYTATITDLPPCIKKALELLNKNENLPHSARFM
                     LATYMLSAGKSVDEVVLLFQNAPDYNEKITKYQVEHLAGKKGSQTKYSVPACSKLANE
                     NLCYATIDCKGITNPIQFGRKNRE"
     gene            379..1539
                     /locus_tag="VF222_11300"
     CDS             379..1539
                     /locus_tag="VF222_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEX7143052.1"
                     /translation="MKKEINIKNNDFLCSLFKEYYFKNIGTLRPPIEMHNREFGLMTF
                     ENKVIRHLAFSKIGNLKAYIIQNVPSDIFCSNAYYQFPTFPINEKGWKGADMIFDIDL
                     NDLILPCQKEHTFYICSNCGVVSRDFTKLCHECQNSKILKNTIPCLRCINALKKETKK
                     LIEFLKKDFGIQKENMEVSFSGNTGFHVVVFQKNYLLLDSKSRSDMVGYLMGKNLSPE
                     SLGVRNDKHGYRIKIPLSGYSYGWRKRIVQKMKISEVSNDHLNKFIKKIGGYQSFKTE
                     IDKIASELGIKIDPQVTTDIHRIFRMVGSLNSKSGLSKIVCYDLDKFDPMNDSCFFEN
                     DRNVDISLTIPLKFSIKGRKYTLDKSISTVPVPVAVFLISKRLANIACYPSS"
     gene            1572..2132
                     /locus_tag="VF222_11305"
     CDS             1572..2132
                     /locus_tag="VF222_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX7143053.1"
                     /translation="MELLIVNNTSPFVNDIVSMVNDLGIPFKCKLYSEIKSEELSLYG
                     AIILSGRRNYNKVTNFKNNKIIDYCNKLDIPLLGICYGAEMINLFFGGSLIKMNNTIH
                     GLVKVTSNNNNIFLPTDSSIYVYQSHRYSIFRLSSELELLCSSSQSKNEVFKHKTRNI
                     FGVQFHPEKSGMDGKSILYNFLRLAN"
     gene            2983..3921
                     /locus_tag="VF222_11310"
     CDS             2983..3921
                     /locus_tag="VF222_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143054.1"
                     /translation="MQDNLSHDLNLPLIEQNISTLPLVIAVVMKYWGEDIINLENKNQ
                     YKSSNIFHGIEIAEKNNFFACFNKSTIKDLRKWIDQGIPPIVIFPGLYDLLQHALLIS
                     GYDDSEKRILTYIPQPDTTGYIPDSKFSSEWNQEDNIAIVILPKDMKSLLSKEKLEST
                     RSYRLCFEAERDFSDGNLELAIKKVTESVNIDRNNAYGWSLLGSCYSETNNRECISCF
                     TKAIKLNSTYFLALRGLGNYYLKTKDYHNAEKYYSQAIDINANRYGPIYKNRAFSRLQ
                     INKNLEAKQDLILYLEKTPNAKDREQITEALKSISI"
     gene            3946..5085
                     /locus_tag="VF222_11315"
     CDS             3946..5085
                     /locus_tag="VF222_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143055.1"
                     /translation="MSRDTKAVAVLVFPTHYSIIKDVKHSAIKKLEENNINIYRIVLE
                     KYFFSIYLNKSIQAANILSTCLGIKKIEIASTSPNNIKSIVNKITNIGQKIIINENFF
                     IKVYSENSNFLSRDIEFIATGLLVEKLSNRLSRPSRNEESASKVMRVYIGNSTSYISW
                     KSLDGIQGNSYGQIRKKAFIIIYNQLSLFSLKKIIEMGFFPDILILYWDYIDLKNKLK
                     SLHQIISNVPTHKLTLKILELNLLIPKRNNNNTDILLNLLIIDICSYFSALIDVVIPF
                     NLILHPIWLIDYSISLCIKNGKIPWMPNLFEDYFKFSTLSENFNNIQVQPNSLKEIKF
                     KDYITVRNKIRIKSDNFGKNIKVFSIKRGSLNIRPNYIDDILNSI"
     gene            complement(5038..6213)
                     /locus_tag="VF222_11320"
     CDS             complement(5038..6213)
                     /locus_tag="VF222_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143056.1"
                     /translation="MVKKVYFTPYGVGLGHASRLITIAEKIQNKNVEVKFSSFGEAVD
                     YISRCGYICHQVPPVEFSWNSQGSFSVKNSISNVPNWFKNFTTQIVKEIEHMIKFSPN
                     LIVSDSRLSSIISGRLLGIPSLVLLNQIKLLLSPKLHSFKAGRLFETVNGEFIGSLWS
                     FANEILIPDLPPPYTISEHNIWDTNSVLKKIKYIGFIAPKNSISEQQITKVTHLLNFS
                     KNKPIVFFHISGPAKTRLPLVRKILDAQKYFKDKIQYVISEGKPNGRIEPLKLSENGW
                     YYEWCPIRDELFILSNILVLRGGHTTISQAIQFGKPIISIPIENHAEQISNSCKLTKI
                     GIGIMLKTNELTSEKIINSINKILNDPKFISRSNEIMGFCSKLDGVQNVINIVRSYI"
     gene            complement(6301..7305)
                     /locus_tag="VF222_11325"
     CDS             complement(6301..7305)
                     /locus_tag="VF222_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143057.1"
                     /translation="MNKTEEINALSSSSSEGINPRRIDHEQNIVSLESNLKTLLDFKS
                     SLLEDQKNGEQVIQELNEKIEVTKKQIDEEREILEGLRIKLKEVNDVKEEEFPKFMEL
                     KELVLKARSQMKIIDEKTSNKPTRERINIKQLTKTLEQLERDIQIKKLSKEEERKLVA
                     RSKEIAIKLHTMKVMHKKEDQYRTISSQYGNLKSKINEIFDQRSDFGNKIGELKKILD
                     TLLDSREKLYEERRKIIRDVRETAAKLEMVNTQINAIEFRKSKITSFGGKRRSRDSHD
                     KNYSGFQSSKDRARRSKENVQIWNSMKEQALKKMSNGEKLTFDEMRLIYSDINNPESF
                     "
     gene            7440..7823
                     /locus_tag="VF222_11330"
     CDS             7440..7823
                     /locus_tag="VF222_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143058.1"
                     /translation="MDDFEKNFPKFDWKNTPAYKHPLGRDCPCPKHEYIREQISIAKN
                     LSESKSNNKHRAQITLKFCPDHYNVYFKEVIPSMPLKHRLMTKFALKLGAIIIQKIPY
                     MQSDQCFYCKFGSGGFDKRQELPPI"
     gene            complement(7820..8521)
                     /locus_tag="VF222_11335"
     CDS             complement(7820..8521)
                     /locus_tag="VF222_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143059.1"
                     /translation="MYFINWERKITKNQYGSNKVVLKETKSFTNFRQFILIFCFTLIS
                     IFSGNPSRPPKIGQTIIENEGFASRNKLKLIGINTPKLFSISYDKIIEEYIEGGNLYS
                     YFPTEKSNDLAFQAGRITGILHKNGFVFIDNKCQNFLVNKDREVIRIDLGFIQKSESL
                     FAQSMDVGSFLASILDLEPQIYRKVAKEFLLGYLQMTNNKLPYLSIILRNILALGFVY
                     SHNNMIKNMLNQRIA"
     gene            complement(8574..8647)
                     /locus_tag="VF222_11340"
     tRNA            complement(8574..8647)
                     /locus_tag="VF222_11340"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8611..8613),aa:Ala,seq:ggc)
     gene            8728..9153
                     /locus_tag="VF222_11345"
     CDS             8728..9153
                     /locus_tag="VF222_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7143060.1"
                     /translation="MNIEKSVGAVIKYQSPSSKMKDPEFLLLRNRRGYWGFPQGHKEE
                     GETEIQTLFREVYEETGINSLNILSFIGRIRYSFFKGEGIKSRKEVSFYYATTNIRDI
                     TISNEHDDFIWTTFSEALTYLNRKQLKSIMIEGHEKGFY"
     gene            9238..10815
                     /locus_tag="VF222_11350"
     CDS             9238..10815
                     /locus_tag="VF222_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7143061.1"
                     /translation="MTTSGKRIVLTADRSLMTNYRGNFLYGFIACGPYELVPEFVFDK
                     LFCPSVETDKHSGEVKVAQCGLRRIEGALLQEFDRKEIFLAHPEMLNKCIGPETKVVG
                     INVMDPLGMAPVTTTMSPEKLSYVAMKFKKMCASIIQLKKKFNFKVVVGGNGAWELAK
                     PDRMRIHGIDTVVIGEADELALDLFHDLEQGDAPELLHTFVRNIENIPPIQGPTINSL
                     IEAMRGCGRGCDFCDVNKRSKKDIPLSRLQEEAKINLKYGFDSIWLQSDEMLLYGCDN
                     KDFFPNADAIIDVWSGLKNVGANFVGTTHMTLSAVASSPELIKKLSQINDMENSGRWL
                     ATNLGVETVAPRMVKKHLGVKTKPFQPEEWGSVVREGCKILNNNHWFPALTLIIGWPD
                     ETPDETQYTIDLIEDFKHMKMRGLVAPLLYQDFSEKNSMHFGNLNEAQFTLFWKCWQH
                     NLRIINDIIPIIIRNKSYGPPMKIFMASLIKVGTWAIMKYLRGLSKDLFEGRLPEDII
                     EHYSRERSVATPLPSKL"
     gene            complement(10838..11176)
                     /locus_tag="VF222_11355"
     CDS             complement(10838..11176)
                     /locus_tag="VF222_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143062.1"
                     /translation="MKFEYEEFSSIEDLFLYMASIAPYMKQLLPITYYKGYLFSIVPV
                     SQLSTDILMMVYVKTDMSTGLFEFDISTGKYKKIQSIERADKNYFLIINPKSATLADK
                     AIANLKDTDQ"
     gene            complement(11203..12045)
                     /locus_tag="VF222_11360"
     CDS             complement(11203..12045)
                     /locus_tag="VF222_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7143063.1"
                     /translation="MVVENKGIISYIRVLKEDLEIIRIIPNDGTVPDFKAGQFVTLSL
                     NNPDEGKVVRRAYSIASPPEKKKYFELLIRWVKKPLPGRLTTQIFNRKEGDEIGWLKP
                     TGIFTVNEKMHDGTPDNRRLVLIGGGTGLAPFIAYSLHLKSIGSKREIIVLHGASYVD
                     ELSYRELLTDLEEESLDKGQDNWNFKYRASISRPHEPFNRSWGGQKGRVESFLRPEPG
                     SNTSTLDEIVGEKITPENSSFYVCGWQGTVDGVLDFLKPKGFVDERHKRKDGSYEIKF
                     ESYG"
     gene            12159..12461
                     /locus_tag="VF222_11365"
     CDS             12159..12461
                     /locus_tag="VF222_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143064.1"
                     /translation="MIKWSNIKDFNNSKNTDLVNSINEQKLEQKKIVVLFEEKMKIFA
                     TERSIESSLDVLNISIELANIRGNLIRSFEQYARNLEEEIVKLNRVVEKNHKSSDL"
     gene            complement(12456..13340)
                     /locus_tag="VF222_11370"
     CDS             complement(12456..13340)
                     /locus_tag="VF222_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7143065.1"
                     /translation="MKIARVKTREEGETYAIVADDFNKIITKNEIQEQTGIPLPLNIK
                     EFLFNNWLDEVYKNYEKLNFQRTISELKLLFPLANPSKIICLAFNYYDHAKDAGLTPS
                     SEPVIFLKPRTALNNPYNDILCPGNVQRLDYEAEIAVIIGKATKKITAEAAMDSVFGY
                     MIFHDVSARDIQFQDKQFTRGKSIDTFAPCGPWITTKDEIIDPQNLKILTKVNGEVRQ
                     NSTSSKMVIPIKKIISSLSQLMTLEPGDIISTGTPAGVAMSMNNPKYLKDGDLVEISI
                     EKLGTIRNKVMFIDKVNL"
     gene            complement(13349..15076)
                     /locus_tag="VF222_11375"
     CDS             complement(13349..15076)
                     /locus_tag="VF222_11375"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7143066.1"
                     /translation="MDISEDIQKLVEITILRNAVDYNGRARLESVISKILGSQPEIKT
                     NIQYVIPKIKEILEQINSLPIERQRELLIQKCPSYLGTKKIVEKNEFHLPPLINAKPG
                     KVVTRFPPEPNGYPHIGHAKAAVIDEEYAKMYSGKLILRFDDTNPLNEKLEYYDAILE
                     GLSWLGIAPHIIKNTSDDIKLLHDYGRKLINKGNAYICLCDQNSIHDLRSKGLPCQCR
                     IHSDKILENSDRFFNGYYHQNEAIIRFKGKMNSNNTAMRDPTLFRIIENTHPKLGNKI
                     IVWPTYDFAAPIEDSLDGVTHAFRTKEYELRNELYQQILKNLKMRIPEVIEFSRLEFE
                     GLPVSKRKLKPLIEEKIVEGWSDPRLPTLMGLKRRGFTPEAIRKFVLSLGITLSETKP
                     SIEVLEAFNRKIIDSKSKRLLFVNDPFKITIKNANPKEVIFKNHPSVEMGNRKIKVNN
                     VVFISTQDAEKLKKGMEIRLMELYNIKVFQIDIAKKEAIVEYTDDIVKENIKKIQWVS
                     DQDLVEFTIVKPNKLFIGDKLNRNSLEVLEGFAESFVTTLSDSTMIQFIRLGFCRIED
                     NKSAIFTHK"
     gene            complement(15078..16079)
                     /locus_tag="VF222_11380"
     CDS             complement(15078..16079)
                     /locus_tag="VF222_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7143067.1"
                     /translation="MKDTTKLEIEDYVNKVNNHLISFLTGEPLELYRASTHYLKSGGK
                     RLRPLMVIKSCEMFEGNQQDALPAASAVEFIHNFSLVHDDIMDNDDMRHGIPTVHKFF
                     GLPIAILSGDILFSKAFQILSTNNINSIKDSSLLSMIRKLSSACIDICEGQYKDIQFS
                     HGLNFPSEDEYIDMISKKTAALFKVSCSLGALCSRNATEKDVNNMSDFGKNSGIAFQL
                     IDDLIGIAGHSKETGKAVGNDIREGKKTYPILLSLKKASEVERAQILKVFGKDECDNV
                     SLKKAIDIISSLQIEKIVRKSAMDYIEKARKAIMNYEDSDPKKILQELSTYIVERRK"
BASE COUNT         5460 a         2465 c         2406 g         5838 t
ORIGIN      
        1 caatttgatt aaaaataaat tccctcagcc cgttaaatat acagctacta taactgatct
       61 tcccccctgt ataaaaaaag cgttggaatt actaaataaa aatgaaaact tacctcactc
      121 tgcaagattt atgttagcca cttatatgct atcagcaggt aagagtgttg atgaggtagt
      181 attacttttt caaaatgctc ctgattataa cgaaaaaatt actaaatacc aagtggaaca
      241 tttagcgggt aaaaaaggca gtcaaacaaa atactctgtt cctgcctgtt ccaaactggc
      301 aaatgaaaat ctttgctatg caactatcga ttgtaaagga ataactaatc ctatacaatt
      361 tggtagaaag aatagagaat gaaaaaagaa attaatatta aaaataatga ttttttatgt
      421 tcacttttta aagaatacta ttttaaaaat ataggaactc ttagaccccc aattgaaatg
      481 cataatcgtg aatttggtct tatgacattt gagaataagg taattaggca tttagccttt
      541 tcaaaaatcg gaaacctaaa agcatatata attcaaaatg ttccttctga tattttttgc
      601 tcaaatgcat attatcagtt tcctactttt cctattaatg aaaaaggttg gaaaggtgcg
      661 gatatgatat ttgatatcga tcttaacgat ttaatattac cgtgtcaaaa ggagcataca
      721 ttttatattt gttctaattg tggtgttgtt tctcgagatt ttaccaaatt atgtcatgaa
      781 tgtcaaaata gtaagatact aaaaaataca ataccgtgtt tgaggtgtat aaatgcctta
      841 aaaaaggaaa ctaaaaaatt aatagaattt ttaaaaaaag attttggaat acaaaaagaa
      901 aatatggaag tatccttttc aggcaatact ggattccatg ttgtagtctt tcagaagaat
      961 tatctgctat tagattctaa atcccgatct gacatggtag gatatttaat ggggaagaat
     1021 ctatctcccg aaagtttagg agtgagaaat gacaaacatg gctatagaat caaaattcca
     1081 ttaagcggat attcatatgg atggagaaaa agaattgttc aaaagatgaa aatctcagaa
     1141 gtatcgaatg atcatttaaa taaatttatc aaaaaaattg gagggtacca atcattcaag
     1201 acagaaattg acaaaattgc ttctgagtta ggaatcaaaa tcgacccaca ggtaacaaca
     1261 gatattcata gaatttttag aatggtaggt tccttaaata gtaaaagtgg actttcaaag
     1321 attgtatgtt atgacctaga caaatttgat ccgatgaatg attcttgttt ttttgagaat
     1381 gataggaatg ttgatatttc acttactata cctttaaaat ttagcattaa aggaagaaaa
     1441 tatacattgg ataaatctat ttcgacagtt ccagttccag tagcagtttt tttgatatct
     1501 aaaagactag ctaatattgc atgttatcct tcttcgtaac atataaattt tataaagaat
     1561 attctatagt tatggagtta ttaattgtaa ataacacctc accatttgta aacgatattg
     1621 ttagtatggt taatgatttg ggtataccat ttaaatgtaa attatattca gaaattaaat
     1681 ccgaagaatt atcattatat ggagcaatca tattatcagg aaggcgaaat tataataaag
     1741 taacaaattt taaaaataat aaaataattg actattgtaa taaattggac atccctttat
     1801 tagggatttg ttatggtgca gagatgatta atttattttt tggtggatca ttaattaaaa
     1861 tgaacaatac tattcacgga ctagttaaag tcacatcaaa taacaataat atttttttac
     1921 ctactgactc ttcaatctat gtatatcaaa gtcatagata ttccatattt agattatctt
     1981 ctgagctgga actcttatgc agttcatcgc aatctaaaaa tgaagtattt aaacataaga
     2041 cacgaaatat ttttggagta caattccatc ctgaaaaaag tggaatggat ggcaaatcta
     2101 tcctctataa tttcttaaga ctagcaaatt agaagatggt gatgctgaaa aaagcttttg
     2161 aagatttagg tattgttaaa attgctaaca ttttcaaaaa tttcttttag agtaaactgg
     2221 ttttatgctt gatatactta tatataatct tgttactaaa ttcatgaaac aaatcaatca
     2281 attatccgaa aacgaataat aatatcattt aaaattatat caaacacatt tacaaatgtt
     2341 tttatttata ttttctaaac ttaaacatat tgttcgttta agataatttc tctctatata
     2401 taattggtca ggaaactatt ttttgtcgat tcacaactct aattttcatt taatatggct
     2461 gctcccaaga aatcataaat taattttact aaaatgataa gaagaatcag ataaatttaa
     2521 gaaacggagc ggatacgatt aatgataaat ttatatattg aatacaaaga aaacaaaaga
     2581 ggtcattata tttaatatga gaataacata tattttccta taattaggtc atattttaca
     2641 taattatata aattattttt tactttaata atataaaata agtaaattcc tttttttaat
     2701 aaaaccctat ttcattgtag agtaataaac attatcgaat tgataattta ttttatattc
     2761 attttcttta agagttgttt ttatgctatt tattatttag aaaatgttat atatagtcac
     2821 atttaaaata aataatcaaa taaacactat attgattaat aaaatgatta acaatcaacc
     2881 ttttataata tgaaatattc tcctactttt gacaaatgat tagttgcgtt aaaggcaaag
     2941 gataggatgg tttttatttg atataaaatt atcttattca aattgcaaga taatctatct
     3001 catgatttga atttgccatt aatagaacaa aatatttcca cattaccatt agtaattgca
     3061 gttgtgatga aatattgggg agaggatatt ataaatttag aaaataaaaa tcaatataaa
     3121 tcctctaata tttttcatgg aatagaaatt gctgaaaaaa ataatttttt tgcctgtttt
     3181 aataagagca caattaaaga tcttagaaaa tggatagatc aagggatccc acctatcgtt
     3241 atttttcctg gcttatatga tctacttcaa catgccctcc taatttctgg ttatgacgat
     3301 agtgaaaaaa gaattttaac ttatattccc caacctgata ccactggtta tatacctgat
     3361 tctaaatttt cgtcagagtg gaaccaagaa gataatattg ctattgtaat actacctaaa
     3421 gatatgaaaa gtttactatc aaaagagaag ttagagtcaa caaggtcata tcgactatgc
     3481 ttcgaagcag aaagagattt ttcggatggt aatctagaat tagctattaa aaaagttact
     3541 gaatcggtca atattgatag aaacaatgcc tatggatggt cattgctggg aagttgttat
     3601 agtgaaacga ataatagaga atgcataagt tgttttacta aagcaattaa attaaattcc
     3661 acttattttc tggcattaag aggtttaggt aattattatt taaagacaaa ggattatcac
     3721 aatgctgaga aatattattc gcaagcaata gatattaatg caaacagata tggacctatc
     3781 tacaaaaatc gtgcattttc cagattgcaa ataaataaaa atctagaagc aaaacaagat
     3841 ttaatattgt atctggaaaa gaccccaaat gcaaaagata gagaacaaat tactgaagca
     3901 ttgaaatcaa ttagtattta aatacaaaat ttttaaaatt ctcaaatgtc aagagataca
     3961 aaagcagttg cagtattggt ttttccaaca cattattcaa taatcaaaga tgtaaaacat
     4021 tcagcaataa aaaaattaga agaaaataat ataaatatat atagaatagt cttagaaaaa
     4081 tatttttttt caatatactt aaacaagtcc attcaagcgg ctaatattct ttcaacatgt
     4141 ttaggaatta aaaaaattga gatagcatct acctctccaa ataatattaa atccattgta
     4201 aataaaataa caaatatagg tcaaaaaata attattaacg aaaatttttt cataaaagta
     4261 tattcggaaa attctaattt tttatcacgt gatattgaat tcattgctac tggtctacta
     4321 gtagaaaaat tatcgaatag attatcaaga ccgtccagaa atgaagaatc agcaagtaaa
     4381 gtcatgaggg tttatatagg aaattccaca tcatatataa gttggaaatc cctggatggg
     4441 atacaaggaa actcttatgg acaaataaga aagaaagcct tcataattat ctataatcaa
     4501 ctttcacttt tttcactaaa aaaaataata gaaatggggt tttttccaga tatcttaatt
     4561 ttatattggg actatattga tttaaagaat aaattaaaat ctttacatca aattataagc
     4621 aatgtaccta ctcacaaact aacattaaaa atattagaat taaatttatt aattccaaaa
     4681 aggaataata ataatactga tattttgtta aaccttttaa taattgatat ttgttcttat
     4741 tttagtgctc ttattgatgt tgttattccc tttaatttga ttttacatcc tatttggtta
     4801 attgactatt caattagttt atgtattaaa aacgggaaga ttccatggat gcctaatctc
     4861 tttgaagatt acttcaaatt tagcactttg agtgaaaatt ttaataatat ccaagtgcag
     4921 ccaaatagtc ttaaagaaat aaaatttaag gattatataa cagtgagaaa caaaatcaga
     4981 ataaaatcag ataattttgg aaaaaatata aaagtatttt ctataaaaag aggttcatta
     5041 aatatacgac ctaactatat tgatgacatt ctgaactcca tctagcttac tacaaaaccc
     5101 cattatctca ttagatcttg atataaattt aggatcattt aatattttat tgattgagtt
     5161 tattattttt tcggaagtca attcatttgt ttttaacatt attccaattc cgatttttgt
     5221 tagtttgcaa gaattactaa tttgttccgc atgattttcg attgggatgg aaattattgg
     5281 tttaccgaat tgaatcgctt gtgaaattgt tgtatgtcct cctcttaaaa ctaaaatatt
     5341 gctcaagata aaaagttcat ctcttattgg acaccattca taataccaac cattctctga
     5401 taacttaaga ggttcgatcc ttccatttgg ttttccctct gatatcacat actgaatctt
     5461 atctttaaaa tatttttgtg catcaaggat ttttctaaca aggggtaatc tggttttggc
     5521 aggaccactt atgtgaaaaa aaacaattgg cttatttttg gaaaaattta gaaggtgagt
     5581 aactttagta atctgttgtt cagaaatact atttttggga gctataaatc ctatatattt
     5641 tatttttttt aatacggaat tggtatccca aatattatgt tcagaaatcg tatatggggg
     5701 agggagatcg gggataagga tttcatttgc aaatgaccat aacgagccta taaattctcc
     5761 gtttacagtt tcaaagagcc ttcctgcctt gaaggagtgt aatttagggg atagaagtaa
     5821 ttttatctga tttagtagaa ctaaagacgg aatccccaat aatcttcctg aaataataga
     5881 agaaaggcgt gaatctgaaa ctattagatt aggagaaaat ttaatcatgt gttctatttc
     5941 tttcactatc tgagtagtaa aatttttaaa ccaatttgga acattagaga tggaattttt
     6001 taccgaaaaa cttccctgag aattccaaga aaattctacc ggtggtactt gatgacaaat
     6061 atacccacat cttgaaatat aatctaccgc ttcaccaaag gatgagaatt ttacctcgac
     6121 attcttattt tgtatttttt ctgctatagt tatcaatcta ctagcatgtc ctaatcctac
     6181 accgtaagga gtaaaatata ccttttttac cattcttaaa cactattata atacaacttc
     6241 tacagataaa atggcgtaat attagacatt tagaaaaaaa cgttaaataa atatagattt
     6301 ttagaaactt tctggattgt tgatatcact ataaatcagt cgcatttcat caaatgttaa
     6361 tttttctccg tttgacatct ttttcaaagc ctgttctttc atagaattcc aaatttgtac
     6421 attctcctta cttcttctgg ctctatcttt agatgattga aatcctgaat aattcttgtc
     6481 atgactgtct ctcgatcgcc ttttacctcc aaacgaagtt atcttcgact tcctaaattc
     6541 tatcgcatta atttgtgtat ttaccatttc taattttgct gcagtttctc taacatctct
     6601 aattatcttt cgtctttctt cgtacaattt ttctctcgaa tccaataagg tatctaatat
     6661 tttttttaat tctccaattt tatttccaaa gtctgatctt tgatcgaaaa tttcatttat
     6721 ttttgacttt aaattgccat attgagatga aattgttcta tactgatctt cttttttatg
     6781 cataaccttc attgtatgta atttaatagc tatttccttt gagcgtgcaa ctagtttgcg
     6841 ttcttcttct ttcgataatt ttttaatttg tatatctcgt tctagttgtt caagggtttt
     6901 agtcaattgt ttgatattta ttctctctct agttggttta ttactggttt tttcatctat
     6961 aatcttcatt tgactcctag cttttaatac taattctttt agttccataa attttggaaa
     7021 ttcctcttct tttacatcat tgacttcttt taattttatt ctaagtcctt cgagtatttc
     7081 tctttcctcg tctatttgct ttttagtaac ttctattttt tcatttagtt cttgtattac
     7141 ttgttctcca ttcttctgat cctcaagtaa tgagcttttg aaatccaaaa gagttttaag
     7201 attggattct agtgacacaa tattttgttc atgatcaatc cttctaggat tgataccttc
     7261 actgctactg ctactcaaag cattaatttc ttcggtttta ttcacgccgc gttaaatttg
     7321 taataaaatt ataaaatata tatttttatc acttgttaac attattattt atattaatca
     7381 ttaggtaaaa tataacctat aatttatatg atattgactt tatattacaa ctgagaagaa
     7441 tggatgattt cgaaaaaaat tttccaaagt ttgattggaa aaatactcct gcatataaac
     7501 atcctctagg aagagattgt ccttgtccta agcatgaata tattagagag caaattagca
     7561 tcgctaagaa tcttagtgaa tcaaaatcta ataataaaca ccgtgctcaa ataaccttga
     7621 aattttgtcc tgatcattac aatgtatatt ttaaagaggt tattccttct atgccgttaa
     7681 agcatagact tatgacaaaa tttgctttaa agcttggtgc tattattatt cagaaaatac
     7741 catacatgca gtccgatcaa tgtttctatt gcaaatttgg gtcaggaggt tttgataaaa
     7801 ggcaagaact tccacccatc taagcgatcc gttgatttaa catatttttt atcatattat
     7861 tatgactata aacaaagcct aaagcaagta tattccttaa gataattgat aaatacggta
     7921 atttgttatt tgtcatttgt agataaccta acaaaaactc ttttgctact tttctgtata
     7981 tttgaggttc taagtctagg atgcttgcga ggaaactgcc tacatccata ctttgagcaa
     8041 ataatgattc acttttctga ataaatccaa gatcaattct tattacttct ctatctttat
     8101 ttaccaaaaa attttgacat ttgttatcaa taaatacaaa tccatttttg tgtagtattc
     8161 cagtaattct acctgcttga aatgctagat catttgattt ttctgtagga aagtaggaat
     8221 agagatttcc accctcaata tattcttcta ttattttatc atatgatatt gaaaaaagct
     8281 ttggagtatt aataccaatt aattttaatt tatttctgga tgcaaaaccc tcattctcta
     8341 ttattgtttg tccgatcttg ggtggacgcg atggattgcc agagaaaata gagattaagg
     8401 taaaacaaaa aattaaaata aattgtctaa aatttgtaaa ggacttggtc tcttttagta
     8461 ctaccttatt acttccatat tgattttttg taatctttct ttcccaattg ataaaataca
     8521 agagaatttt attatttatt tgtttatatt attttaatga tacctttagg tagtggaccg
     8581 ggtgggattc gaacccacga tctcagcaat gccaatgccg tatcctaccg gactagacga
     8641 ccggccctaa caatgaatta aattaactcg atattaatat ttttaagaaa caatattata
     8701 attctgaatt caaaaaatta gtgtagtgtg aatatagaaa aatctgtagg agcagtcata
     8761 aagtatcaat caccttcaag taaaatgaaa gaccctgaat ttctattact aagaaatcgt
     8821 agaggatatt ggggttttcc ccagggccac aaagaggagg gagaaacgga gatccaaacc
     8881 ctatttagag aggtatatga agaaactgga ataaattctt taaatatatt atcttttatt
     8941 ggaagaatta ggtattcatt ttttaaaggt gaaggtatca aatctagaaa agaagttagt
     9001 ttctattatg ctactactaa cattagagat ataaccattt caaatgaaca tgatgatttt
     9061 atttggacaa ctttttcaga agctttaaca tatctcaata gaaagcaatt aaaatcaata
     9121 atgatagaag gacatgaaaa gggattttat tgaaattttc caacactccc acctagtaac
     9181 tttatgataa taaatttata tctgattctc tgaggatgag tttagaggat tattgtaatg
     9241 actacttctg gtaaaaggat agttctaact gctgaccgaa gtctaatgac taattatcgt
     9301 gggaattttc tttatggttt tatagcctgt ggaccttatg aattggtacc agagtttgtt
     9361 tttgataaac ttttttgtcc aagtgtagaa acagataaac attcaggaga agtgaaagta
     9421 gctcaatgtg gattgagaag aatcgaaggt gctttacttc aagagtttga tagaaaagaa
     9481 atttttctcg cacatcctga aatgttaaac aaatgtatcg gaccagagac taaggtcgta
     9541 ggaattaatg taatggaccc tttaggaatg gctccggtta caacgacaat gtctcctgag
     9601 aaactatcgt acgtagcgat gaaatttaaa aaaatgtgcg cttcaattat acagttaaag
     9661 aaaaaattta atttcaaagt tgtagtagga ggaaacggtg cttgggagct tgcaaaacca
     9721 gatagaatga gaattcatgg aatagatact gttgttatcg gagaagcaga tgaattagct
     9781 ttggatttat tccatgactt ggaacaagga gatgctcctg agcttctaca tacctttgtc
     9841 cgaaatattg agaatattcc tccgatacaa ggtcctacaa ttaattcttt aatcgaagca
     9901 atgcgcggat gcggacgggg ctgtgatttt tgtgacgtaa ataaaagatc aaaaaaagat
     9961 attcctcttt cgagattaca ggaagaagcg aaaattaatc tcaaatacgg gtttgactcg
    10021 atatggcttc aatctgatga aatgctattg tatggatgtg ataataagga tttctttcca
    10081 aatgctgatg ctattataga tgtctggagt gggttaaaga atgtaggagc aaattttgtt
    10141 ggtactactc atatgaccct ttcggctgtc gcgtcgtcac ctgagttgat taaaaaactt
    10201 tcacaaataa atgatatgga aaatagtgga agatggttag ccaccaacct tggggttgaa
    10261 actgttgctc caagaatggt caaaaaacat ttaggggtta aaactaaacc atttcaacca
    10321 gaagaatggg gatcggttgt tagggaaggt tgtaaaattt taaataataa tcattggttt
    10381 cctgctttaa ctcttataat tggatggcct gatgaaacac cagacgaaac ccaatatacc
    10441 atcgatctaa ttgaggattt taaacatatg aaaatgcgag gattggtagc tcctttactc
    10501 tatcaggatt tttcagagaa aaactctatg cattttggta atctaaatga agctcaattt
    10561 acccttttct ggaagtgttg gcaacataat cttcgtatta ttaacgatat cataccaatt
    10621 ataatcagaa ataaaagtta tgggccacct atgaagatat tcatggccag tttgattaaa
    10681 gtaggcactt gggcaataat gaagtatctg agaggtcttt ctaaggatct ttttgaaggt
    10741 cgattacctg aagatattat agaacactat tcaagagaac gttcggtagc tactccattg
    10801 ccatctaagt tataaaaaag tatcttttta tactttttta ttgatcagta tcttttaaat
    10861 ttgcaatcgc tttatctgct aaggttgctg attttggatt tattattaag aaataatttt
    10921 tatctgcacg ttctattgat tggatttttt tatatttgcc agttgaaata tcaaattcaa
    10981 ataaaccagt agacatgtct gttttgacat aaaccatcat taatatatca gtagaaagct
    11041 gcgaaactgg aactattgaa aaaagatacc ctttataata cgttattggt aatagttgtt
    11101 tcatgtacgg tgctatagaa gccatataaa gaaacaaatc ttctatagat gaaaattcct
    11161 catactcaaa tttcataatt agatttcact tgataaataa ttttatccgt atgactcgaa
    11221 tttaatctcg taacttccat cttttctttt atgcctttca tcaacaaatc ctttaggctt
    11281 taagaaatct agcacaccat caactgttcc ttgccatcca cagacataaa atgaactatt
    11341 ttcaggtgtg attttctcac cgacaatctc atctaaagtt gatgtattac ttcctggttc
    11401 aggtcttaaa aaagattcaa ctctaccttt ttgccctccc caggatctat taaaaggttc
    11461 atgtggtcta ctaatacttg ctctatattt aaaattccag ttatcttgtc ctttatctag
    11521 actttcttct tccaaatcag ttaataactc tctataactg agttcatcta cataacttgc
    11581 tccatgcaag acaattattt cccttttact accaatagac ttaagatgta aactatacgc
    11641 tataaaagga gctagtccag ttcctcctcc aattaataca agtctcctat tatcgggagt
    11701 cccatcatgc attttttcat ttactgtaaa tatacctgtt ggttttaacc aaccaatctc
    11761 atctccttcc ttacgattga aaatctgagt agttaatctg ccaggtaatg gtttcttcac
    11821 ccatctgatc agtaattcaa aatatttttt tttttcgggg ggagatgcta ttgaataagc
    11881 tctcctaacc actttacctt catctggatt attaagagac agtgtaacaa attgcccagc
    11941 cttgaaatct ggaactgtcc catcgtttgg tataatacgt ataatctcga gatcttcttt
    12001 taaaactcta atataagaaa taatcccttt attctccaca accattggta atatcggaaa
    12061 taatttctct ttagtcctta tatatgttat tatactatta aatgtaataa ttggagtcga
    12121 aaattttatc ttttgttatt cttgatgaaa tataaaatat gatcaagtgg tcaaatataa
    12181 aagattttaa caattcgaaa aacacagatt tagtgaattc aattaatgaa caaaaattag
    12241 aacaaaaaaa aatagtagtt ctttttgaag agaaaatgaa aatatttgca acagaaagat
    12301 ccattgagtc tagtctggat gtcttgaata tttcaattga attagctaat atacgaggaa
    12361 atttaattcg atcctttgaa caatatgcta gaaacttgga ggaagaaata gtaaaactaa
    12421 atagagtggt agaaaaaaat cacaaaagtt ctgatttata gatttacctt atctataaac
    12481 attactttgt ttcttatggt gccaagtttt tctatactaa tttctactaa atctccatct
    12541 ttcaaatatt ttggattatt catggacatt gccacacccg caggggtacc ggttgaaatg
    12601 atatcgcctg gttccagagt catgagctga ctcaaactgg atattatttt ttttataggt
    12661 atgaccattt tactgcttgt agaattctgt cgtacttccc cattaacctt ggttaatatt
    12721 tttaaatttt gagggtcgat tatttcatct tttgttgtta tccatggccc acacggtgca
    12781 aaagtatcta tactttttcc tcttgtaaat tgtttatcct gaaattgaat atctcttgca
    12841 gatacgtcat gaaatatcat atatccaaag actgaatcca tcgctgcttc tgctgtaatc
    12901 ttctttgttg ctttacctat tataactgct atttctgctt catagtcgag gcgctgaaca
    12961 ttccctggac ataaaatgtc attataagga ttatttaatg ctgttcgggg cttcaaaaat
    13021 attactggtt cagaagaggg ggtgagtcca gcatctttcg cgtgatcata gtaattaaat
    13081 gccagacaga tgattttact tggattagct agcgggaaga gtagcttcaa ctcactaatc
    13141 gttctttgga aattcaattt ttcataattt ttataaactt catctaacca attattaaat
    13201 aaaaattctt ttatattaag aggaagtggt ataccagttt gttcttgtat ctcattcttt
    13261 gtaatgattt tattaaaatc atcagccact atggcatatg tttccccctc ttctcttgtt
    13321 tttactctag ctattttcat tttatctcct acttatgagt gaatattgct gatttgttat
    13381 cttctattct acaaaagcca agtcttataa attgaatcat tgtagaatca gataatgtag
    13441 tcacaaaaga ctctgcaaaa ccttcaagta cttctagact attccgattc aatttatcgc
    13501 ctataaataa tttatttggc tttacgatcg taaactctac caggtcctga tctgacaccc
    13561 attgtatttt tttgatattc tcttttacta tatcatctgt atattctact attgcctctt
    13621 ttttggctat gtcaatttgg aaaactttga tattgtataa ttccatcaat ctgatttcca
    13681 tgcctttttt taatttttca gcatcttgag tggatatgaa tacgacatta tttaccttaa
    13741 tttttctatt tcccatttca acggatgggt gatttttaaa tataacttct ttagggtttg
    13801 cattttttat agttattttg aagggatcat taacaaataa taatctcttt gatttagaat
    13861 caattatctt tctattaaat gcctctaaca cttctatcga tggtttagtt tctgaaagag
    13921 ttataccaag cgataataca aatttgcgta ttgcctctgg tgtaaaacct cttcttttta
    13981 atcccattaa agttggaagc ctgggatcgg accacccttc aactattttt tcttcaataa
    14041 gaggttttaa tttgcgtttg gagacaggca gtccttcaaa ctctaatcgt gaaaactcaa
    14101 ttacttccgg tattctcatt ttgaggtttt ttaatatttg ttgatataat tcatttcgca
    14161 attcatattc tttagttcga aatgcatgag taacaccgtc taaactatcc tcgatgggtg
    14221 cagcaaagtc atatgttggc caaacaatta ttttatttcc taactttgga tgagtatttt
    14281 caataattct gaatagtgtt ggatctctca ttgctgtatt attactattc atttttcctt
    14341 tgaatctgat tatagcttca ttttgatggt aatatccatt aaaaaatcta tcggaatttt
    14401 ctaatatttt atcagaatgt attctgcatt gacagggaag tccttttgac ctcaaatcat
    14461 gaatactatt ttgatcacat aaacaaatgt aggcatttcc cttgttaatt aatttcctac
    14521 cataatcatg taacaattta atatcatctg aagtattttt gattatatga ggtgctatac
    14581 ctaaccatga taatccctcc aaaatagcat cataatattc taatttttcg tttaagggat
    14641 tggtatcatc aaaacgtagt attaattttc ccgaatacat tttagcatat tcttcatcaa
    14701 taacggctgc cttcgcatga cctatatgag gatacccatt tggttcagga ggaaatcggg
    14761 ttacaacttt tccaggcttt gcattaatta gaggtggtaa atgaaattca tttttttcaa
    14821 ctattttttt tgttcctaaa tagctaggac atttttggat taataattct ctttgtctct
    14881 ctataggtaa tgaattgatt tgttcaagta tttccttgat tttgggaatt acgtactgga
    14941 tgttagtttt tatttcgggt tgggaaccta atattttgga gataacggat tctaatctgg
    15001 ctctgccatt ataatcaact gcatttctta aaatggtgat ctctaccaat ttttgtatat
    15061 cctcggaaat atccatacta ttttctcctc tctacaatgt aggtagataa ttcttgaagt
    15121 atttttttag gatctgagtc ttcataattc atgatggctt tcctagcttt ttcaatataa
    15181 tccattgcac tttttcttac aatcttttca atttgaagtg aagaaattat atctattgct
    15241 ttttttaaac tgacattatc acattcatcc ttaccaaaaa ccttcagaat ctgagctctt
    15301 tctacttctg aagctttttt gagtgagagt aagataggat acgttttttt tccttctcta
    15361 atatcgtttc ctactgcttt tccagtttct ttagaatggc ctgcgatccc aattaaatca
    15421 tcaattagct ggaatgcaat acctgaattt ttaccaaaat cactcatatt atttacatct
    15481 ttttctgttg catttctcga gcaaagtgct cccaatgaac atgagacttt gaatagagcg
    15541 gctgtttttt tcgaaatcat atcaatatat tcatcttcag atggaaaatt taggccatga
    15601 gagaattgga tatccttgta ttgaccttca cagatgtcaa tacaagcaga ggaaagcttt
    15661 cttatcatgc taagcaaaga actatccttt atagagttaa tgttattagt ggagagaatt
    15721 tgaaaagctt ttgagaaaag aatatcaccg gataagattg caataggcaa tccaaaaaat
    15781 ttatggacag ttggtattcc atgcctcata tcatcattat ccattatatc atcatggact
    15841 agagagaaat tatgaatgaa ttccacggcg gaagcagctg gtaatgcatc ttgttgattt
    15901 ccttcgaaca tttcacaaga ctttatcacc attagtggtc taagacgttt tccaccactt
    15961 tttaaataat gagtggacgc tcgatatagt tctaaaggct cccctgttag aaaagagata
    16021 aggtgattat taactttatt aacgtaatct tcaatttcta atttggtggt atccttcatt
    16081 gttaataacc atgtctgcta attcaccagt taatataagt cttgatttag ctaaatctgc
    16141 aatatttgaa gatccagtaa gaaacattg
//
LOCUS       DASVEV010000259         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_525786, whole genome shotgun sequence.
ACCESSION   DASVEV010000259 DASVEV010000000
VERSION     DASVEV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525786"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VF222_11385"
     CDS             <1..1201
                     /locus_tag="VF222_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7143068.1"
                     /translation="KDILNLLENITEHPKLTYILDLCKKEGLDISANKNHNLLLNDGQ
                     NSQILIFSHYRDTATHIVEELNKTGIKSFRFVGQSTRNNDIGMNQNEQSVILDSFRKK
                     KFNVLVATSIAEEGLDIPEVKLVIFYEPVASEIRHIQRKGRTGRKSSGNVIILAAKDT
                     VDMRILFASKRRIEKMKTIFKSMKTILNPISRVSAPYNRLTELELVDLDKNLKIEQKK
                     LNEKELKYEDNERKVIEKIKTKKEIIDKVPKFQIGTITKNENNLIEKASRHIYDFLID
                     ERNNKNLFDLSYFHEKFTFDTHIILQALKKLEKQQVIKLKNNRSITLNRLPNKTTGTE
                     YNIYVEKILPGKAYVLVNERWYALLNYYDYDGPRNLLKKGSKFKAIAELYTNGSDFCI
                     RIKEIRL"
     gene            1830..>2029
                     /locus_tag="VF222_11390"
     CDS             1830..>2029
                     /locus_tag="VF222_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7143069.1"
                     /translation="MQFANNMIIDLSQSINSDIKLYPGSPNVYFLKWSKYTIDGYDSE
                     AIFLSTHTGTHMDAPSHFIEGA"
BASE COUNT          821 a          266 c          295 g          647 t
ORIGIN      
        1 gaaggatatt ctcaacctat tggaaaatat aacagagcat cccaaactca cttatattct
       61 tgatctatgt aaaaaagaag gattagatat ttctgcaaat aaaaaccaca atctattatt
      121 aaatgatggc caaaattctc aaattttgat attttcacat tatagagata ctgcaactca
      181 tattgtagag gaattgaaca aaactggaat caaatcattt cgatttgttg gtcaatctac
      241 tagaaataac gacattggaa tgaatcaaaa tgagcaatct gttattttag attcatttag
      301 aaaaaaaaaa tttaatgtac ttgttgcaac atcgatagca gaagaaggtt tagatattcc
      361 tgaagttaaa ttagttatat tttatgaacc agtagctagt gaaattagac acatacaaag
      421 aaaaggtaga actggaagaa aatcaagtgg aaacgtaata attctagcgg caaaagatac
      481 tgtagatatg cgtattttat ttgcaagtaa gagacgaata gaaaaaatga aaacaatttt
      541 taaatcaatg aaaacaattt taaatcccat ttcacgagtc tccgcaccat ataatcgtct
      601 gacggaatta gaattggtag atcttgataa gaacttaaaa atagaacaaa aaaaattaaa
      661 tgaaaaagaa ttaaaatatg aagataatga aaggaaagta attgaaaaaa tcaaaaccaa
      721 aaaagaaata atagataaag ttccaaagtt tcaaattgga acgataacaa aaaatgagaa
      781 taatttaata gagaaggcat ctagacatat ttatgatttt ttaatagatg agagaaacaa
      841 taaaaattta ttcgacttat cctattttca tgaaaaattc acatttgata cacatattat
      901 attacaagcc ctaaagaaat tagagaagca acaagtaatc aaattaaaaa ataatagatc
      961 aataacttta aatcgattac caaataaaac aacaggaaca gaatataata tctatgtgga
     1021 aaaaatttta cctggcaaag catatgttct agtaaatgaa agatggtatg cattactaaa
     1081 ctattatgat tatgatggac ctaggaacct tttaaagaag ggatcaaaat ttaaagctat
     1141 tgcagaatta tatactaatg gaagtgattt ttgcattaga ataaaggaaa taagacttta
     1201 attaaactca tccgaccagt tttaggacaa tttattcgtt taatcttatg aaaagattat
     1261 acctttaaat tcatcagata tatagcctct tctctgggag agatacagaa ggctatagct
     1321 tacattatta aatatatggt ttcgggtcaa tttgctggat gaaaatgtgt ttttacatgt
     1381 tcagagcaat aatgaaataa acatctagga cattgtttta ttggtgagtt acgacaaagc
     1441 attatgccac aaaccaaccc tttcttagaa ttatttctcc attcattatt atcaaaacga
     1501 tcactcatct aagtgagtaa tgtttaatga atagatatta aactttaata atttaacaaa
     1561 attatctcca agtgttaaat actatataca cacattaaaa gttcatacaa aatgttctac
     1621 tctgatgtct tagccttctt ccctgatcta gatatccttg gctcctaata ctagataaag
     1681 agagggttat ggcattttaa aatattcgaa taatatagta tttatagtga ttaatgcaaa
     1741 ttaaatagtt atattttaaa acacttattt gatatcgtct tcttagtcat taaattatct
     1801 tgtattcttc tgaaataatt aaaagggaga tgcaatttgc aaataatatg attatagatt
     1861 taagtcaatc aataaactct gatattaaac tttatccggg atctccgaat gtctattttt
     1921 taaagtggtc taaatatact attgacggct atgattcaga agcaattttt ttaagcacac
     1981 atactggaac tcatatggat gctccttctc attttattga aggagctga
//
LOCUS       DASVEV010000260        11565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1691407, whole genome shotgun sequence.
ACCESSION   DASVEV010000260 DASVEV010000000
VERSION     DASVEV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691407"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..204)
                     /locus_tag="VF222_11395"
     CDS             complement(34..204)
                     /locus_tag="VF222_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HEX7143070.1"
                     /translation="MTKGTTSMGKYTRKKVHIRCRRCGHNSFHKRGKACSKCGYPDPK
                     MRKYNWIKRQVM"
     gene            complement(251..478)
                     /locus_tag="VF222_11400"
     CDS             complement(251..478)
                     /locus_tag="VF222_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HEX7143071.1"
                     /translation="MSIDMAVKVLDESFGKIVLIKLKGGKVIRGTLHGFDQHMNLLLD
                     DAVEIVEGKSSDLGTIVVRGDNVVIISPPPV"
     gene            complement(602..1312)
                     /locus_tag="VF222_11405"
     CDS             complement(602..1312)
                     /locus_tag="VF222_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7143072.1"
                     /translation="MNNIYRLSNTDMEKWKKKSDQIIIPIGSLEQHGAHLPVSTDSII
                     IEHLAKNIAEKIQILYIPAIPFGISFEHEPLFNISLSNNTFSNLIGDICVSLAKYGMK
                     NIILLNGHHGNMGSMYYISQNVSHKIPSNTRLNFINYWNLMKDFDHAGEIETSLLLAI
                     NTKLVKMKMARANTKEIDGSKVAYTSLTSHPGSFPKITGNGVWGDPTKASIIKGRSLL
                     EDLIQKLEQTIIELRKFS"
     gene            complement(1447..2343)
                     /locus_tag="VF222_11410"
     CDS             complement(1447..2343)
                     /locus_tag="VF222_11410"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX7143073.1"
                     /translation="MNQKKIKETKKTLIEITVIGKDRKGVVAEITNFIFKNYGNIEKI
                     NQNVIKGLFGMQLEASFNRIIKEDLDRGLAVLAKDLKMEIKVHYQEPNRLTNIAILIS
                     NEDHCVLKILESYHKKELKSKIQIIIGSSNLLKPITDKYNIPFYLVDHKDQLQAEGQI
                     LKLFEEYNIDMVVLARYMRILTPNFVWRYPNKIINIHPSLLPAFPGAYAYVQAYERGA
                     KIVGCTAHFVTEELDQGPIICQKSFNVMDTDDIDSIKNKGRRLESSTLLEAVKLALEG
                     KLEVYWGKVHIKKTSKKKIKLL"
     gene            complement(2389..3795)
                     /gene="glmM"
                     /locus_tag="VF222_11415"
     CDS             complement(2389..3795)
                     /gene="glmM"
                     /locus_tag="VF222_11415"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7143074.1"
                     /translation="MTIKIKENKSNTQRLFGTNGVRGIYGRELTFPLIIDLTYSLATY
                     FKTGPIVVGYDGRKSSPILSNIVSAVLNAGGLDVIMAGQLPTPCLQFAAKSKKCGGGI
                     MVTASHNPPEYNGIKPCATDGVEISREDELKVEDIFYNRKFSKTDQTNKTFADHNIIN
                     EYMERVLSLIDVEKIKSRSFKVVMDLGNGIQALVAPYLLKKLGCQIFTINSNIDGDFP
                     GRGPEPTVDNLTSLSNMVRDLNADFGVAYDGDGDRSLFCDEKGIIHPGDKTAAVIIKY
                     LINGKHTGSEIICPINTTLNVQLVADEGNSNVVYTKVGSVEVSREMVKRNALIGLEEN
                     GGFMYGKLNQVRDGAMTTGLVLDMLTNFSLANKTTNSFSEIIASLKKIAQFKTKFKCP
                     SKEIANKIVTKCLEHGSPKKIEKLDGAKIWIDDESWIMIRPSGTEPLVRMYGESISQT
                     LLDSKVKEYTRIIESVLQ"
     gene            3850..4016
                     /locus_tag="VF222_11420"
     tRNA            join(3850..3882,3924..3930,3972..4016)
                     /locus_tag="VF222_11420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3927..3929,aa:Leu,seq:tag)
     gene            complement(4237..4437)
                     /locus_tag="VF222_11425"
     CDS             complement(4237..4437)
                     /locus_tag="VF222_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143075.1"
                     /translation="MKAQIKRNISNNSKTPQKSKNKNFDYNKPVGKCMICYTLSGWHC
                     YDCGNDYCQNHFHIHKESNQCS"
     gene            4997..5383
                     /gene="rpl7ae"
                     /locus_tag="VF222_11430"
     CDS             4997..5383
                     /gene="rpl7ae"
                     /locus_tag="VF222_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HEX7143076.1"
                     /translation="MSKPYYVKFEVPKDLVSAIYEALRIAKDSGKIRKGTNETTKAVE
                     RGVSKLAIIAEDVEPPEVVAHLPIICEERNAPYVFVPSKQQLGEAMGISVGSAAASII
                     DAGESQHILDEIVNSTKNKNTQSPNP"
     gene            5407..5622
                     /locus_tag="VF222_11435"
     CDS             5407..5622
                     /locus_tag="VF222_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HEX7143077.1"
                     /translation="MSKTVEEKVVPAEVIQIVGRTGIAGEVIQVRVRVLEGKDKGRIL
                     TRNVKGSVRMNDVLMLRETEREARKIK"
     gene            5669..5884
                     /locus_tag="VF222_11440"
     CDS             5669..5884
                     /locus_tag="VF222_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HEX7143078.1"
                     /translation="MSKTATSLRNCFFCGQRISSASGIMLVKNDGAIQWTCSNKCKKN
                     LRKLRRDPRKQKWTNKYVKGGIKVSKS"
     gene            5905..6306
                     /gene="ndk"
                     /locus_tag="VF222_11445"
     CDS             5905..6306
                     /gene="ndk"
                     /locus_tag="VF222_11445"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7143079.1"
                     /translation="MSEQTLIVIKPDAFERKLTGIILSRFEDKGFLIKELKSYIFTKE
                     KAEEFYDAHKNKPFFSELVSFISSSNVVACILEGENAINIVRLMIGTTQSFEAQPGTI
                     RGDYGLGITKNIIHASDSYESFIKESQVIFK"
     gene            6314..8143
                     /gene="infB"
                     /locus_tag="VF222_11450"
     CDS             6314..8143
                     /gene="infB"
                     /locus_tag="VF222_11450"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7143080.1"
                     /translation="MNLRQPVVVVLGHVDSGKTSLLDKIRGTGVQAREAGGITQHIGA
                     SFFPIETIKTITGPLYERLTKTDEQIPGLLFIDTPGHEVFANLRSRGGSAADIAIVVA
                     DVNKGFEPQTIESIEILKKRKVPFVVALNKIDMVSGWKGKKKGESSRSRKENTLNLGF
                     ISEEIKKQDSNILTLLDEKIYSVVGSLSRMGFNSEAFWRIKEFDKELAIVPISAVSGV
                     GIPELMAVLVGLAQQYMKKRLERRYDYVRGIILEINEELGLGPSANMILLDGTIKQGD
                     MIMVAKRDDIVITKVKALLLPKPLDEMRDPRDKFRSVTEVISAAGLRIVSPDLEGVLA
                     GSPLYVLRNQEEEKEIRHLIELEIKDTFINNTNSLGVILRCDTIGSLEAIIDMLKKEN
                     IPIQSADIGHIIRRDVLAAHAVKDKDRYLGVILGFNVKVLDDAKKESTESDVHIFNEK
                     VIYNLVRNYTDWVKYQKEHADSIMFEEIPPICKIHFLKGFIFRRNDPAVFGAEILVGK
                     LRQKNHVINEHGSKVGTIHQIQDKGKNIDEAKKGMQVAVSIRGPTIGRQINEGDFFYT
                     DLNSRHAKLLNEKFGHRLNEEEKEIFDFILNLKRKSDPAYGYI"
     gene            complement(8390..8740)
                     /gene="trxA"
                     /locus_tag="VF222_11455"
     CDS             complement(8390..8740)
                     /gene="trxA"
                     /locus_tag="VF222_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7143081.1"
                     /translation="MQANTNNNDDLIIELSEENFEESINGNKPILVDFWATWCGPCKF
                     MLPVFDKLAKKYSSKIAFGRLNVDDSQSIAVKYDVYAIPTFIIFSKGQTVERAVGAVG
                     EKGLETSLEKFFKS"
     gene            complement(8810..9379)
                     /locus_tag="VF222_11460"
     CDS             complement(8810..9379)
                     /locus_tag="VF222_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX7143082.1"
                     /translation="MEEKFRYLDHMTDLIVEAYGNTLQELFENSAFGLINAMFDMSNV
                     ELSHSIKIIAQGHDFKSLLYDWIEKIILAIYIDRMIVVKFDSLSFSKIITNDHTNNNN
                     KENNNNNITIIKEMEFLANDLDLYDSDKSTKSQPDNYIYKIEAIGNGDIIDLQKHEYK
                     IEVKSITYHEMEIKRDEDGYYIARFLVDL"
     gene            9486..10736
                     /gene="hisS"
                     /locus_tag="VF222_11465"
     CDS             9486..10736
                     /gene="hisS"
                     /locus_tag="VF222_11465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX7143083.1"
                     /translation="MRDISQEDVFYINYIKEKFYETIASYNFQVIEPSPIELLSTLEA
                     KSGESIINEVYSFKDKGGRDIALRYDLTIGITRFAISKRDIRMPIKLANFGGVFRYDE
                     PQAGRYRYFHQWDIEIYDNFKTDAEAEIIEFVSNFFAKLNLETIIEISDRRILEEYIK
                     IKYGLDDENSIGNLFRAIDKVPKKGVRQVCTEYKDIVDEQILQDLLEISSFKGGILDL
                     TSHGKLKNLKSISYLQELFLSLELRGIQNIRVNLGVVRGLDYYSGIVFEIFDKVSNVG
                     SLVGGGRYDKLTRVFGRPDIGAVGAAGGVERIILALQYHKKLPNNIKKILYIANSNES
                     VRNSALSILSKLRKKGFTVEYDLSNRSLRKQLYDASSKGVSLTIIVAPEEISKNNVII
                     RNMKDGKEIVENIEYLDKKIPQFL"
     gene            complement(10731..10988)
                     /locus_tag="VF222_11470"
     CDS             complement(10731..10988)
                     /locus_tag="VF222_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143084.1"
                     /translation="MVIKYSKRVFLAPNTSDQEIMDKAIKILISNGINNNDIQINYNT
                     NELEEGDLYISYDPPDLVIRNVYQKKSSGIIKMKSISMIKL"
     gene            11263..>11565
                     /locus_tag="VF222_11475"
     CDS             11263..>11565
                     /locus_tag="VF222_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7143085.1"
                     /translation="MTDSIQHDVVIIGSGLAGLRAAIAAAAYGNDNISIGVFSKVQVM
                     RSHSVSAEGGTAAVLFEDEGDNIESHIYDTIKGSDFLADQDVAEKLVKTIPHEVYQL"
BASE COUNT         3868 a         1797 c         1862 g         4038 t
ORIGIN      
        1 ttatttatat tctttcttat tcattatttt ccatcacatt acttgacgtt tgatccagtt
       61 atactttctc atttttggat caggataacc acattttgaa catgcttttc cccgcttatg
      121 gaatgaatta tggccacatc ttctacatct tatatgtacc ttcttgcgtg tgtatttgcc
      181 cattgaagtt gttccttttg tcatgttctc atatcaacct cctactcttc ttcgacattc
      241 ttcccttatc ttacacaggg ggcggggata ttataactac attatcccct cttactacaa
      301 tagttcccaa atctgatgac ttaccttcta ctatctctac agcatcatca agtaataagt
      361 tcatgtgttg atcgaaacca tgaagagttc ctctaatcac tttaccaccc tttaatttta
      421 tcagtacaat ttttccaaaa ctctcatcca aaacctttac ggccatatca attgacaata
      481 ctaattctac ctgtaactat gaaatgaata ttagttatat attaaaattt cattcaaata
      541 cattaatttt tttattagtg aaatattact taataaaaaa atcgagaaat aactatatga
      601 gttaactaaa ctttcgaagt tctattatag tttgctcaag cttttgaatt aaatcttcca
      661 acaacgatcg tccctttatt attgatgctt ttgtgggatc accccagact ccattccctg
      721 tgatttttgg gaaagaacca ggatgtgatg taaggcttgt ataagcaact tttgatccgt
      781 caatttcttt ggtatttgcg cgtgccattt tcattttgac caactttgta ttaatagcta
      841 ataaaagaga tgtttcaatt tcaccagcat gatcaaagtc cttcattaga ttccagtaat
      901 ttataaaatt gagtcttgta tttgaaggaa ttttatgaga aacattttga gatatataat
      961 acatagatcc catgttccca tgatgaccat ttagcaagat tatatttttc attccatatt
     1021 ttgccaatga tacacagata tctccaataa gattggagaa agtattgtta gatagtgata
     1081 tattaaacaa gggttcatgt tcaaaggaaa taccaaaagg tatagcggga atatataaaa
     1141 tttgaatttt ttcagcgatg tttttggcta aatgttctat tatgatggaa tctgtagata
     1201 ccggaagatg agcaccatgt tgttctaacg atcctatagg aattattatt tgatcggatt
     1261 tttttttcca tttttccata tcagtgttag ataacctgta aatgttattc attttgtaaa
     1321 tctatccctc caactcctac tcctctgtgt acttcttcct atcaatagtt cttttatcaa
     1381 tttttataat gtttgtattt atttttatag tttaaaaata acaacatatg tatgtcatga
     1441 agatatctat aataatttta ttttcttctt cgatgttttt ttaatgtgaa cctttcccca
     1501 ataaacttca agttttcctt ccaaagctaa ttttaccgcc tcaagcaatg ttgaactttc
     1561 taatcttctt cctttgtttt taattgagtc tatgtcgtct gtatccataa cattaaaaga
     1621 tttctggcat atgattggcc cttgatccaa ttcttcagtt acaaaatggg ctgtacaacc
     1681 tacgattttt gctcctcttt cgtaagcttg aacataagcg tatgcaccag gaaaagcagg
     1741 taataatgat ggatgaatat tgattatttt attaggatat ctccatacaa aatttggggt
     1801 aagaatcctc atatatctag caagaactac catgtctatg ttgtattcct cgaataattt
     1861 caaaatttgt ccttctgctt gtagctgatc tttgtgatca accaaataga aaggaatgtt
     1921 atatttatcg gttattggtt ttaatagatt cgagcttcca attataatct gaatttttga
     1981 tttaagttcc tttttatggt acgattcaag tatttttaat acacaatgat cttcattact
     2041 tatgagaata gcaatattag ttaatctgtt tggttcttgg taatgcacct tgatctccat
     2101 ctttaaatct ttcgcaagta ctgctaaacc tctatcaaga tcttccttta ttattctatt
     2161 gaatgaagct tcaagctgca taccaaagag tccttttatt acattttgat ttattttttc
     2221 aatgtttcca taattcttaa agataaagtt agtaatttct gcaactacac cttttctgtc
     2281 ctttccgatg acagttatct cgattaaagt tttcttagtt tctttaatct ttttttgatt
     2341 caacattttt ctaccataat tttgttttgt tttttgatat ttttctattt attgtaacac
     2401 actttctatt attcttgtgt attctttcac ttttgaatcc aggagtgttt gtgagatcga
     2461 ttctccgtac attcttacca gtggttcagt tccacttggt ctaatcatta tccacgattc
     2521 atcgtcaatc caaattttgg ctccatccaa cttctcgatt tttttgggtg atccatgttc
     2581 taaacatttg gtaactatct tatttgctat ctccttggaa ggacatttaa atttggtttt
     2641 gaattgcgct atttttttta gtgatgctat gatttcagaa aatgaattag ttgttttatt
     2701 ggccaatgaa aaattggtaa gcatatctaa aaccaatcct gttgtcattg ctccgtctct
     2761 tacctggttt aatttaccat acataaatcc tccattttct tctaaaccaa taagagcatt
     2821 tcttttgacc atctctcggg atacttccac actacctact tttgtatata caacgtttga
     2881 atttccttca tcagcaacta attgaacatt caaagttgtg tttataggac atataatctc
     2941 tgaaccggta tgttttccat ttattaaata cttgataatt acagcagcag ttttatctcc
     3001 tggatgaata attccttttt catcacagaa taaacttcta tccccatctc catcataagc
     3061 tactccaaaa tctgcattca aatctctcac catgttagat aggctagtta aattgtcaac
     3121 agttggttct ggtcctctcc ctggaaaatc tccatcaata ttactgttta ttgtaaagat
     3181 ttgacaaccc aattttttta acaaatatgg tgcaaccaga gcctgaatac cattgcccaa
     3241 gtccattact actttaaaag atctagattt tattttttca acatctatta aagataaaac
     3301 tctttccatg tactcattta taatattgtg atctgcaaaa gtcttatttg tttgatctgt
     3361 tttcgaaaat tttcgattat aaaatatatc ttcaacctta agctcatcct ctcttgatat
     3421 ctctacacca tccgtagcac aaggtttaat tccattgtat tcaggtggat tatgtgaagc
     3481 tgtaaccatt attccaccac cgcatttttt tgatttagca gcaaattgta aacagggagt
     3541 aggtaattga ccagccatga ttacatccaa accacccgca tttaatacag ctgatacaat
     3601 gtttgataaa ataggacttg actttcttcc atcgtaacct acgacaatgg gtccagtttt
     3661 aaaatatgtt gctaatgagt atgtcaaatc tattatcaaa ggaaaagtta attctcttcc
     3721 gtatattcct ctaacaccat tagtcccaaa taaacgctga gtattagatt tattttcttt
     3781 tattttaatc gtcataatat attaatttaa gaatagataa atttggctat tataaattgt
     3841 atttcaagtg cgggggttgc caagccaggc caacggcgca ggtctgtctt tttttctcag
     3901 atgagataga aggacagtgc gaggcttaga aagaaaagta atggtcctcg gctacaaact
     3961 agtttcctac aaccctgttt cttagggatt cgtgggttca aatcccacct cccgcattac
     4021 aaaagttctc atggaaaagt agaacgagaa ataatatttg gaattttttg ctttttttta
     4081 tttttaaaaa cacatttatt ttgaatttta tgcgtgataa attaaaataa taaagaggta
     4141 cattttactt tactcaataa ctaattaaat taccactatt ataacaagct catccattat
     4201 agtttatata atttggaata atattctcct taaattttac gagcattgat tactttcttt
     4261 atggatatga aaatggtttt gacaataatc gttaccacag tcataacaat gccagcctga
     4321 taatgtataa caaatcatac attttccaac tggtttgtta tagtcaaagt ttttattttt
     4381 agatttttgg ggtgtcttag aattgtttga tatattacgt tttatttgtg ccttcattct
     4441 gatctaccta attaataaac ctctctctca ctcattcaat ctctctcttt tacaaaacca
     4501 taccatagat gtaggtattg ttcaaaatta ttttgttagt cttgatgcta tttacgattg
     4561 tctccttcaa actctataat tatatttaac ataattattt aaggttaacc ccatgaatat
     4621 aactaaaaat caaattatac aataatagtt ccacttccta actactgaag aactcataac
     4681 atctggcttc atcaactcaa tcaccaactg tttatttttg ttgttatttc aataactata
     4741 atattttgat gttctattaa aagaactttt gatgtaaata gtgaaggtta ataaagttaa
     4801 gatatacaat ttatttttgt agtagaatat ttttatttaa aatcaccaaa ttcttatttt
     4861 attagattta gacaaatcaa aaattaaaat tttatctata ttgataaaaa atttcgaaat
     4921 tctcctaata gaggtaacat tttaataggc tctattttgt aaagagatgg atgatcccaa
     4981 agggtgatat tgaataatga gtaaaccgta ttatgttaaa tttgaagtac caaaagattt
     5041 ggtaagtgct atttatgaag cactaagaat agcaaaagat agtggaaaaa taagaaaagg
     5101 aactaatgaa acaactaagg cagttgaacg aggtgttagt aaacttgcta ttatcgcaga
     5161 agacgtggaa ccaccagaag tagttgctca tctcccaatt atatgtgaag aaagaaatgc
     5221 tccctatgtc tttgtaccaa gtaaacaaca actaggggaa gcaatgggaa taagtgttgg
     5281 ttcggcagct gcatccatta ttgatgctgg tgagtcccaa catattttag atgaaatagt
     5341 taactctacc aaaaataaaa atacacaatc tcctaatcca taataaaaag tcaggtgtta
     5401 tagttaatga gtaaaacagt agaagagaaa gttgtacctg ctgaagtaat tcagatcgtg
     5461 ggtagaaccg gtattgcagg ggaagtaatt caagtacgag tcagagtttt agaaggaaaa
     5521 gataaaggcc gaattttaac gagaaatgtg aaaggctccg ttagaatgaa tgacgtattg
     5581 atgctccgag aaactgaaag agaagcgaga aaaataaagt aatataaata atataaaaac
     5641 agagaaaaat aatgaggttt aattaaaaat gagtaaaaca gctacttctt tacgcaattg
     5701 tttcttctgt ggtcaacgta tttcttctgc tagcggaatt atgttggtca agaatgatgg
     5761 agctattcaa tggacttgtt caaacaaatg caaaaaaaac cttcgaaagt taagaagaga
     5821 tcctcgaaag cagaaatgga ctaacaaata cgtcaagggt ggaattaagg tctctaaatc
     5881 ctaataaata gaaaagatac gtagatgagc gagcaaacat taatagttat aaaaccagat
     5941 gcttttgaac gcaaactcac tggaataatt ttgtcgcgat ttgaggataa aggattccta
     6001 attaaagaac ttaaatcata tatttttaca aaagaaaaag cagaagagtt ttatgatgca
     6061 cacaaaaata aaccattttt cagtgaactt gtgtccttta tctcatcttc taatgtagtt
     6121 gcctgtattt tagaaggaga gaatgcgata aatattgtaa gactgatgat cggcacgact
     6181 caatctttcg aagcgcagcc aggcacaatt agaggagatt atggtcttgg tattacaaaa
     6241 aatatcatcc atgcttctga ctcatatgaa agttttataa aagaatctca agtcattttt
     6301 aagtagtaac aacgtgaacc tccgacagcc agtagtagtt gtattgggtc atgtcgactc
     6361 aggaaaaact tccttattag ataagattag aggtacagga gtacaggctc gtgaagctgg
     6421 aggtataact caacatatcg gagcaagctt ttttccaata gaaacaatta agacaataac
     6481 tggaccttta tatgaacgac ttactaaaac cgatgagcaa atccctggcc ttttatttat
     6541 tgatacacct gggcatgaag tgtttgctaa tcttagaagt agagggggat cagcagcgga
     6601 tatagcaata gtagttgcag atgtaaacaa aggttttgag ccacagacaa tagaaagtat
     6661 agagatacta aaaaagagaa aggttccttt tgttgtggca ctaaataaaa tagatatggt
     6721 ttcaggttgg aaagggaaaa aaaagggaga atcaagtcgc agcagaaagg aaaatacact
     6781 taatttagga tttatatcgg aagaaataaa gaaacaggat tctaatattc ttactctttt
     6841 agatgaaaaa atttacagtg ttgtcggatc actgtcacga atgggcttta attctgaagc
     6901 cttttggcgc attaaagaat ttgataaaga gttggctata gttccaatta gtgctgtttc
     6961 tggagttggg ataccagaat tgatggcggt acttgttggc ttagcccaac agtatatgaa
     7021 aaaaaggttg gagagacgtt atgactatgt tagaggtatc attctagaaa tcaatgaaga
     7081 acttggccta ggcccttctg ctaatatgat actattagat ggaactatta agcaaggaga
     7141 tatgatcatg gttgcaaagc gagatgatat agtaataact aaagttaaag ctttactcct
     7201 tccaaagcct ttagatgaaa tgcgtgatcc acgtgataaa tttaggtcag tgactgaagt
     7261 aatatcagcc gcgggtttaa gaatagtgtc tcctgactta gaaggtgtat tggccggcag
     7321 tcctttatat gttttaagaa atcaagaaga agaaaaagaa attcgacacc ttattgaatt
     7381 agagatcaaa gatactttta ttaataatac taattcgtta ggtgtgattc ttagatgtga
     7441 cacaattggg tcacttgaag ctataattga tatgttgaaa aaagaaaata tacctattca
     7501 aagtgccgat attggacata taatcagaag agatgttttg gcagcgcatg cagttaaaga
     7561 taaagataga tatcttggag tgatattagg ttttaatgtt aaagttctag atgatgctaa
     7621 aaaagaatct acagaaagtg acgttcacat ttttaatgaa aaagtaatct ataacctggt
     7681 aagaaattat actgactggg taaaatatca gaaggaacat gctgattcta ttatgtttga
     7741 ggaaattccg ccaatttgta aaatacattt tttaaaagga ttcatattta ggaggaatga
     7801 cccagctgtt ttcggagcgg aaatacttgt aggaaaactg agacagaaaa atcacgttat
     7861 aaatgagcac ggatctaagg tgggaacaat tcatcaaatt caagacaaag gaaaaaatat
     7921 tgacgaagca aaaaaaggta tgcaggttgc tgtatctata agaggtccta ccattggaag
     7981 acaaataaat gaaggtgatt tcttttatac cgatctcaat agtaggcatg caaagctatt
     8041 aaacgaaaag tttgggcata gactcaatga agaagaaaaa gaaatatttg attttatatt
     8101 gaatttaaaa agaaaatcag atccagctta tggttatatc tgactatata tttataaaag
     8161 agatatttta gacttgttga gttttgtaag cctaattcac tctattataa atcaggtgca
     8221 ttgatgatta aattttcagt caattcaaat gaaaaacaag ataaataatc atagcagtac
     8281 aaaaactata tcactcaata ggaaaagaat ttcccatttt attgttagat aattctcagc
     8341 agcagtaatg acatcaagtt aactaacttt tattatattg aaaacgtcct taagatttaa
     8401 aaaatttttc cagggatgtt tctaatcctt tttccccaac agctcctact gcccgttcga
     8461 ctgtctgtcc tttagaaaat atgataaacg taggaattgc atagacatca tattttacag
     8521 caatagattg agagtcgtct acattgagcc ttccaaaagc gatcttagag gaatattttt
     8581 ttgccagttt atcgaataca ggtaacataa atttacatgg accgcaccat gtggcccaaa
     8641 agtcaacaag aatgggttta ttaccattta ttgattcttc aaagttttct tcagataatt
     8701 ctattattag atcatcgtta ttatttgtat tagcttgcat gtatccttga tcatacctta
     8761 agtttataaa attatataaa attattggag aaagttagta tatcatatgt cacaaatcta
     8821 ccaaaaacct tgcaatataa taaccatctt cgtccctttt tatttccatt tcgtgatatg
     8881 ttatagattt tacctctatt ttgtattcgt gtttttgtaa atcaattata tctccatttc
     8941 caatagcttc tattttatag atgtaattat ctggttgtga cttagtagac ttgtcagaat
     9001 catacaaatc cagatcattt gctaaaaatt ccatttcttt tatgattgtt atattattat
     9061 tattattttc tttattgttg ttattcgtat ggtcattggt aattattttt gagaatgaaa
     9121 gagaatcgaa cttgaccaca atcattctgt caatgtagat tgctaaaatg attttttcta
     9181 tccaatcata gagtaagctt ttaaaatcgt gtccttgtgc aattatcttg attgaatggc
     9241 ttaattctac attagacata tcaaacatgg catttattaa tccaaaagca gaattttcga
     9301 atagttcttg taaagtatta ccataagctt ctactattag atctgtcata tggtctagat
     9361 acctgaattt ctcttccata cgtgaataca ctgctttaaa ctatattata taaataatga
     9421 aaaatcggaa aatctaaatt ccaatatttt caatttttat tgtgaagctg gacttaccaa
     9481 gagggatgag agatatatcc caggaagatg tcttttatat aaattatatt aaagaaaaat
     9541 tttacgagac catcgcctca tataattttc aagtcattga accctcacct attgaactat
     9601 tgtcaacact tgaagcaaaa agcggggaat caataataaa tgaggtttat agttttaagg
     9661 acaaaggtgg acgagatatt gctttacgtt atgaccttac aatagggata accagattcg
     9721 ctatctcaaa aagagatata cgtatgccta taaaattagc aaattttggt ggagttttca
     9781 gatatgacga acctcaagct ggcagatatc gttattttca tcaatgggat attgaaattt
     9841 atgataattt caagacagat gcagaagccg aaataataga atttgtttcg aattttttcg
     9901 ccaaacttaa ccttgaaaca attattgaga taagtgatag aagaatcctt gaagaatata
     9961 taaaaataaa atacggcctt gacgacgaaa attccatagg aaaccttttt agagctatag
    10021 ataaagttcc aaaaaaagga gtcagacaag tatgtacaga atacaaggac attgttgatg
    10081 aacaaattct tcaagacctt ttggaaattt cttctttcaa aggagggatt ttggatctta
    10141 cttctcatgg gaagttaaag aatttaaaaa gcatcagtta tttacaagag ttatttttat
    10201 cattagaatt gagaggtatt cagaatataa gagtaaatct aggtgtagtg agaggattag
    10261 actattattc tgggattgtt tttgagatat ttgacaaggt ttccaatgtg ggttctttag
    10321 taggaggagg gaggtacgat aaacttactc gtgtatttgg acggcctgat attggtgcag
    10381 taggtgcagc tggaggagta gaacggataa ttttagcctt gcaatatcat aaaaaacttc
    10441 caaataatat taaaaaaatt ctatatattg ctaatagcaa tgaatcagtt agaaattcag
    10501 ctctttcaat tttaagcaaa ttaagaaaga aaggctttac tgtagaatat gacttatcca
    10561 atagatcgct tcgaaagcaa ctttacgatg cttcctctaa gggagtatct ctgactataa
    10621 ttgtcgctcc agaggaaatt tcaaagaaca atgtaattat tagaaatatg aaggatggaa
    10681 aagagatagt tgagaatata gaatatttag ataaaaaaat tcctcagttc ctataatttt
    10741 atcatagaaa tacttttcat ctttatgatt ccactacttt tcttctggta gacatttcgt
    10801 atcaccagat ctggaggatc ataactgata tagagatctc cttcttctaa ctcatttgta
    10861 ttatagttaa tttggatatc attattattt ataccattag agatgagaat ttttattgct
    10921 ttatccatta tttcttggtc tgaagtattt ggagcaagga atactctctt agaatatttt
    10981 ataaccataa taaaataaat acaaattact tactagtaat aaatgtaaat cttgaaaact
    11041 catctgctca ttttttttct aattgactaa gttacttttg ttgatttatc acacaaaatt
    11101 caatttcttt tgttttaatc tttttttctt tatttttgta tttcttttta atagagacat
    11161 tatcaaaact taagtaatta taaattacat catgaaatat tctcatgaat taatcataaa
    11221 gtatatttgt attatcctag tacacttttt tgggataaat tagtgacaga ttcaattcaa
    11281 catgatgttg ttattattgg gtcaggccta gctggcttga gagctgcaat tgctgcagca
    11341 gcatatggaa atgataatat cagtataggt gttttttcca aagttcaagt gatgcgatct
    11401 cattctgtat ccgctgaagg aggtactgca gcagttttgt ttgaagatga aggagataat
    11461 atagaatctc atatatatga tacaattaaa ggaagtgatt ttttagcgga tcaagatgtg
    11521 gctgaaaaac tagtaaaaac aatacctcat gaagtgtatc aatta
//
LOCUS       DASVEV010000261         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1693783, whole genome shotgun sequence.
ACCESSION   DASVEV010000261 DASVEV010000000
VERSION     DASVEV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693783"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..707)
                     /locus_tag="VF222_11480"
     CDS             complement(162..707)
                     /locus_tag="VF222_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX7143086.1"
                     /translation="MSNDKIIRLIKGDITDQNVDVVVNPANSYLKHGGGVAGAIVRKG
                     GKIIQTESDKIGFVPVGSSVITTSGKLPCHAIIHTVGPKMGEGNEDFKLSKSLHSSLS
                     LASDNSYKSISIPAISSGIFGFPKDRCANIILDESIKFLQNNNTSLQTIEFCIIDDET
                     LFHFKTKFKKVKKNFSSPDNL"
     gene            complement(881..1621)
                     /locus_tag="VF222_11485"
     CDS             complement(881..1621)
                     /locus_tag="VF222_11485"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7143087.1"
                     /translation="MDNINKSKVFQNIKDKIIDLDEFVFLTGAGISQESGIPTFRGPE
                     GLWKKYDPMKLASISGFYENPKLVWEFYKDRQELICNCNPNLGHISIADFEKIKNSSV
                     LTQNIDGLHKKAGSKNIVELHGNILRMECTECEFQETIEKKIGQPLPPVCKICKNILR
                     PSIILFGEPLNPDIWEEAERLSNGCDVMFIVGTSLNVGPVNQLPLYAKKNDAILIEVN
                     PEPTIFNKIMNYSVQGSATEILPQLLHL"
     gene            complement(1874..2308)
                     /locus_tag="VF222_11490"
     CDS             complement(1874..2308)
                     /locus_tag="VF222_11490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7143088.1"
                     /translation="MTQQQECIFCKIALKKIPTNIIIENDKAMAFLDAFPLSKGHALV
                     IPKAHYSKIQELDENSSQSLFNILWKITNPIEKAMRVNSSTIAIHNGKEAGQEIPHVH
                     IHVIPRESGDGAGPVHSMFKNKPNVSSSDMASIIAEIKKSLT"
     gene            complement(2654..3790)
                     /locus_tag="VF222_11495"
     CDS             complement(2654..3790)
                     /locus_tag="VF222_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143089.1"
                     /translation="MLKDSKNNHESNDKMSLKFDIQRYLDVSIIPSFDDDDNHFFGIK
                     DAIPITLKVESFIFIKSYCIHNIHHIFRLPVKTEIKWKINGGTDNGAFKIGGGMDAEY
                     FARDDGDSVIFYPQLKNTSKDTTYKNICIYLTINQLGSTLFKQQEYKFIVNVNILIIR
                     KFLKRNYRVKMFVSKLDDDDNYDDENNNKVDDNNNNNSISNSHISSPADDYDLKSIGK
                     VEQDGDLILFKSVNSCKTCNLMVIVKSNKTSFYIKFFKKHLITTEIVKISAESNNTTT
                     KNNYNNNNSSVLLTGSKYKKYFLEGVKIEPRLFWKSNSGKLVYGYLGQSLIYYTPGED
                     DLSQSPLTINLYFQYDHNLEDLQKANLLDSKKIWILRQPPLMGG"
     gene            complement(4179..>4671)
                     /locus_tag="VF222_11500"
     CDS             complement(4179..>4671)
                     /locus_tag="VF222_11500"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143090.1"
                     /translation="NYGWGGISELATRLGKSVSYVDKRIRLLDLPPDVVESLSTGSLS
                     VSIGDELLPLKEKEQQSKLAELIKQRKLSSRQVRELKSEMKISDNPYDVLDFQTRIID
                     IDAKTQKSFDKAIIAIRVAMTKLASVINDVEDNWTVYEILLQHKNMLHNQIDILYKEK
                     KKI"
BASE COUNT         1566 a          662 c          605 g         1838 t
ORIGIN      
        1 aaaataaagg accaggagac aagactccag aagtagatac taatataact tgaagtgaaa
       61 attctattgg agacatatct tctctttcat ctctaaagtt cttactagct tataatatgg
      121 aaagtttata ataagtgtac ataatacaga tatattacta tctaaagatt atcgggagaa
      181 gaaaaatttt tctttacttt tttaaatttc gttttaaaat gaaaaagtgt ttcatcatct
      241 attatacaaa attcaattgt ttgaagagat gtattattat tttgcaaaaa tttgatagat
      301 tcatctaaaa taatattagc acatctatct ttgggaaatc caaatattcc tgaacttatt
      361 gcaggaattg aaatactttt ataactattg tctgaagcta gggaaagaga actatgaagt
      421 gattttgaaa gcttgaaatc ttcgtttcct tcgcccatct tcggaccaac agtgtgaatt
      481 atagcatgac atggtaattt cccagatgtt gtgattacag atgaacctac tggaacaaat
      541 cctatcttat cactttctgt ttgaattatc ttgcctcctt ttctaacaat agcaccagca
      601 acacctcccc catgtttcaa ataagagttg gctggattga ctacaacatc aacattttga
      661 tcagttatat ctccttttat taaacggata attttatcat ttgacattct aatttcatca
      721 attaatttag ttgttatcat aattgatata ggtaataata aaaatatata taaataaaga
      781 atttactaac tttagtaagt tatataataa attctcattt agagtataat tgattgatta
      841 taaaaaaata tttattaatt attttaatat acttattttt ctataaatgc aataactgtg
      901 gtaatatttc tgttgcactg ccttgaactg aataattcat gattttatta aatattgtag
      961 gttcaggatt aacttcaatc aaaatagcat catttttttt agcataaagt ggtaattgat
     1021 taacaggccc tacattcaat gaagttccta ctataaacat tacatcacag ccatttgata
     1081 atctttcagc ttcctcccat atatctggat tgagcggttc tccaaataaa ataatagatg
     1141 gtcttaggat atttttacaa attttacaaa ctggtggtag tggttgacca attttctttt
     1201 caattgtttc ttgaaattca cattctgtgc attccattct taaaatgttt ccgtgtaatt
     1261 ctacaatatt tttactacct gcttttttat gtaatccatc aatattctga gttaatacag
     1321 acgaattttt tattttttca aaatctgcaa tagatatatg acctagatta ggattgcaat
     1381 tacatattaa ttcttgtcta tctttataaa attcccaaac taattttgga ttttcataaa
     1441 aaccagaaat ggaggcaagt ttcataggat catatttttt ccataatcct tcaggtccac
     1501 gaaaggtagg tattccactt tcttgggata ttccagctcc ggtaagaaaa acgaattcat
     1561 ctaaatctat tattttatct ttgatgtttt gaaatacttt tgatttatta atgttatcca
     1621 ataatgaata ttgatttttt acctatataa aaataaaaaa aaatattttt tttgctataa
     1681 gcaaatcatc atttaatttt taaaaatatt acaatttgat actacaatag tatagatgat
     1741 gggtgatgat ataaatataa aataataatg ataaatagca gatgttgctt gctcttttga
     1801 ttttaatact ttgccttgta ttgtctttgt tttggtataa ttgtaaaatt acaccataaa
     1861 cagattgaac aaattatgtt aaagattttt ttatttctgc aatgatagaa gccatatcag
     1921 aactactaac atttggttta ttcttaaaca ttgaatggac tggtcctgca ccatcaccac
     1981 tctctctggg aataacatga atgtgaacat gtggaatttc ttgtcctgct tcttttccat
     2041 tatggatagc tatagttgag ctgttaacac gcatagcttt ctcaatgggg ttagttattt
     2101 tccataatat attgaatagt gattgtgatg aattttcgtc taactcttga attttggagt
     2161 aatgagcttt gggtattaca agggcatgcc ctttggataa tgggaacgca tccagaaaag
     2221 ccattgcttt gtcattttca ataattatat ttgttggtat ttttttaagt gcaattttac
     2281 aaaaaataca ttcttgttgt tgtgtcaatt tctacaataa taaaagtaag agaggaatat
     2341 aaaatcatgt atattattat aataacaaat taccatttta tatttgtcaa aaagatcgat
     2401 attataaatg gtatttcttt agaagatttc tttattatag ttattattat tagtatttat
     2461 taatgttaaa aattcttaat tagcatgttt tcacatttat tatactaatt ctactcataa
     2521 atatttaata gtaattttgt tttttaaaat tatagttatt gattggtcag acagaaatga
     2581 aaaaaaataa taaataaata agtaaataat gattttatta aatcgttata ataattacta
     2641 agaacatata ctctcatcct cccattaaag gtggttgtct taatatccaa atctttttac
     2701 tatctaaaag attcgctttt tgtaaatctt ccaaattatg atcatattga aaatataaat
     2761 ttattgttaa tggagattgt gacaaatcat cttcaccagg agtataataa attagagatt
     2821 gccctaaata tccgtataca agtttccctg aattggattt ccaaaataac cttggttcaa
     2881 tttttacacc ctctaaaaaa tattttttgt actttgatcc agttaataga acagaactat
     2941 tattgttatt ataattattt tttgtagtag tattgttgct ctctgcagaa atcttgacaa
     3001 tttcagttgt tatcaagtgt tttttaaaaa attttatata aaaagaagtt ttattagatt
     3061 tgacaataac cattaaatta caagttttgc aactgttaac ggatttaaac aatattagat
     3121 ctccatcttg ttctacctta ccgatagatt ttagatcata atcatcagca ggacttgaaa
     3181 tatgagaatt tgatatagaa ttgttgttgt tattatcgtc tactttgtta ttattttcat
     3241 catcataatt gtcatcgtcg tcaagcttac ttacaaacat ctttaccctg taatttcttt
     3301 ttaaaaattt tcttattatc aaaatattta cattaacaat aaatttgtac tcttgttgtt
     3361 taaataaagt tgatcccaat tgattaattg tcaagtaaat gcagatattt ttgtaagttg
     3421 tatctttgct ggtatttttt aattgaggat aaaaaattac agaatcacca tcgtctcgag
     3481 caaaatactc agcatccatt ccgcctccta ttttgaaagc accattatca gtccctccat
     3541 ttattttcca tttgatttcc gtctttacag gtagtctaaa aatgtgatga atattatgta
     3601 tacaatatga tttaataaaa ataaaacttt ctacttttaa tgtaataggt attgcatctt
     3661 taataccaaa gaaatgatta tcatcatcat caaaactagg aattatacta acgtcaagat
     3721 acctttgaat atcaaacttt aaagacatct tatcgtttga ttcatgatta ttttttgaat
     3781 cctttaacac tttagattac attatgtgtg aaatatcttt taaattttga ggttttaata
     3841 tgaatgtata tttattgatt ttattgttat tattattatt tttatattct tctaattgca
     3901 tctcagaaca atttgtgcaa agatcatcat tgacaacaca gatgtgatgt tggtataaac
     3961 agtagtaatc tgattaaaat tcatccttct ttattttttt cttttaatgt attaataata
     4021 cgttggacct ttttttagtt aacaatcaag gttacagaat aatatattta ttatttttag
     4081 atattcatga attaatagag atatgcatac agacttcctg aaaaatgcat agtataaaaa
     4141 gagaaatatt tttcggcgcc gaaaaatttc cggcagactt agatcttttt cttttccttg
     4201 taaagaatat ctatctggtt gtgtagcatg tttttgtgct gtaaaaggat ttcatataca
     4261 gtccagttat cctctacatc attgataaca gaagccaatt tggtcatggc aactcttatg
     4321 gcaataatag ctttatcaaa tgatttttgt gtttttgcat ctatatcaat tattctagtt
     4381 tgaaagtcta agacatcata ggggttgtca gaaattttca tctcactctt taactcacgg
     4441 acttgtcttg atgatagttt tcgttgcttt atcaattcag ctaattttga ttgttgttct
     4501 ttttccttta atggcagcaa ttcatctcct atacttacac ttaaactacc tgtagaaaga
     4561 gattcaacaa catctggtgg tagatcaagt aatcttattc gtttatctac ataactaaca
     4621 ctttttccca atctagtagc taactctgat atgcctcccc atccataatt t
//
LOCUS       DASVEV010000262         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_530332, whole genome shotgun sequence.
ACCESSION   DASVEV010000262 DASVEV010000000
VERSION     DASVEV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530332"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF222_11505"
     CDS             <1..663
                     /locus_tag="VF222_11505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7143091.1"
                     /translation="QSIAKRFFSNRNAASYDRIVKYTTFGRDSLWKQNIANYITPEHK
                     NILDVACGTGIFSSFISKIPYKNLIGVDLTYSYISNAKAKREYSLLVNSMAELLPFKP
                     ESFDCIVSCYLAKYAKISIVVDEIWRLLKKGGLVIFFDFVYPLNKIMRLLWNSYFNIL
                     KLAGQIIRSWKYVFYNLDEVIKQTTWTKDLKIALEKKGFRSLDVKYYTGKTSAIMSAF
                     KI"
     gene            660..1397
                     /locus_tag="VF222_11510"
     CDS             660..1397
                     /locus_tag="VF222_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143092.1"
                     /translation="MNDNNFKSIRINEWFVPRFGPLKFRAFIGMLFLPYTGMCISFTI
                     IGSMLSESIAWDRVLSIFIIYFLALGVSAHAADNLGSKKIKPWGTFFSTLELRLMVIG
                     GLSVSYILGIYYIITFAPLLLIIAVIEGFFLFAYNFELFNGFFHNNFWFAVSWGGVPL
                     LAGFVIQTNSISILALLSSIIAFLVAYTEIKISRKYKELKRKSQDIIDCNTKKLEHSL
                     KIISLITISFAVLLIVCRYFIEYYNIF"
     gene            1496..1912
                     /locus_tag="VF222_11515"
     CDS             1496..1912
                     /locus_tag="VF222_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143093.1"
                     /translation="MKLNSFEKILLMNTGTTEQLLQILFNSLTKIVILQQAEKNNIIT
                     RKINIISKDREQILASASSTIYLKNLPNKIIYEIRKGEKGIGMIILENKLETYKRITE
                     IGYNSKESYIYRKYTLLSGKHIISKVIENFSIEISN"
     gene            complement(1994..2344)
                     /locus_tag="VF222_11520"
     CDS             complement(1994..2344)
                     /locus_tag="VF222_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143094.1"
                     /translation="MNRSDDTSTKNHTRYEKIYKIKTFNDSIKFYLPRVEGYNDIIKD
                     ITLKYGALSIFEFDGYFEGKFEPTKYIRVEIHTNDVNTENMIEFANEVRINLKQKSLA
                     FEFNNNLILVESDN"
     gene            2448..2696
                     /locus_tag="VF222_11525"
     CDS             2448..2696
                     /locus_tag="VF222_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143095.1"
                     /translation="MTNKPTKNNISSSHRKNEKNKAKEILKSRELDQLEELSIRIDTL
                     VELLSDKGIINKKGYINNVMMRIHETSKAKSFDECDEI"
     gene            complement(3090..3566)
                     /locus_tag="VF222_11530"
     CDS             complement(3090..3566)
                     /locus_tag="VF222_11530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7143096.1"
                     /translation="MNNLDNRTAALNKYQLPVPKNSIERIDRNTSPAHIGKLRNAIDF
                     IVPENTPVLAATDGIITFVKDDSKIGGASPAYWNFSNFISIMHKNNEISRYDHLKFNS
                     SNVKEGQKVKQGQKIAEVGLTGYTYIPHLHFQIFIFTGPNIWTDFDTLIVEDFVDS"
     gene            complement(3730..3933)
                     /locus_tag="VF222_11535"
     CDS             complement(3730..3933)
                     /locus_tag="VF222_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143097.1"
                     /translation="MSNLIVESYIKSTINTILDDIAKKYSIDTAKEHLTEDMPVKEIK
                     FRYRTYTECVRILYRNAGYLKEI"
BASE COUNT         1501 a          488 c          522 g         1560 t
ORIGIN      
        1 caatctattg caaaaaggtt tttttctaac cgaaatgctg ctagctatga tcgaatagta
       61 aaatatacta catttggaag agattcattg tggaaacaaa atattgctaa ttatataact
      121 ccagaacata aaaatatact agatgttgca tgtgggacag gaattttttc ctcattcatt
      181 tctaaaatac catacaaaaa ccttatcggg gttgatttaa cctatagtta tatatctaat
      241 gctaaagcaa aaagagaata ttcacttttg gtaaattcta tggcagagtt attaccattt
      301 aaaccagaaa gttttgattg tatagtgtct tgttatttgg ctaaatatgc aaaaatttct
      361 atcgtagttg atgaaatatg gcgtctactc aaaaaaggtg gattagttat tttctttgat
      421 tttgtatatc cattgaataa aataatgaga cttctgtgga attcctattt taatattctg
      481 aaattggctg gccaaattat tagatcatgg aaatacgttt tttataattt agatgaagtt
      541 ataaaacaga ctacatggac taaagatcta aaaatagcat tagaaaagaa agggtttcga
      601 tctcttgatg ttaaatatta tacaggcaaa acttctgcaa taatgtctgc atttaaaata
      661 tgaatgacaa caattttaaa tccattcgta taaacgaatg gttcgttcca agatttggcc
      721 cattgaaatt cagggctttc attggaatgc tatttcttcc atatactgga atgtgtatct
      781 cttttacaat aataggttca atgctttcag aatctatagc atgggataga gttctatcga
      841 tctttattat ttatttttta gctcttggtg tttccgctca tgctgcagat aacctaggtt
      901 caaaaaaaat taaaccttgg ggtacttttt tttctacctt ggaattgaga ttaatggtaa
      961 taggagggct ttctgtgtcc tatatattag gcatttatta tattattaca tttgctccat
     1021 tgttattaat tattgcagtt attgaaggct tttttttatt tgcttataac tttgaattat
     1081 ttaatggatt ctttcataat aatttttggt ttgcagtatc atgggggggt gtacctttat
     1141 tagcaggatt tgttattcaa actaattcta tttctatatt agccttactt tcatctatta
     1201 tcgcattttt ggtagcatat acagaaataa aaatttctag aaaatataaa gaattaaaaa
     1261 ggaaatcgca agatattatt gattgtaata caaagaaatt agaacattca ctaaaaatta
     1321 taagtttaat aactatatca tttgcagtgt tactgattgt atgtcgatat tttattgaat
     1381 attataatat attttaggtt taacattaat taattaaatg tatatgttca ttattcatat
     1441 taaatctaat tctactattt taaatgatat tttaaaactc gagaagacaa gtagtatgaa
     1501 gttaaattct tttgaaaaga ttcttttaat gaatactggt acaacagaac aattattaca
     1561 aattttattt aattcattaa ctaagatagt aattctgcaa caagctgaaa aaaataatat
     1621 tataactagg aaaataaata taatttctaa agatagagaa cagattcttg catctgcaag
     1681 ctctactatt tacttgaaaa accttcctaa taaaatcata tatgaaataa gaaaaggtga
     1741 aaagggaata ggtatgatta tattggaaaa taaattagaa acatataaaa gaattacaga
     1801 aattggttat aactcaaaag aatcctatat ttatagaaaa tatacattat tatcgggaaa
     1861 acatattata agtaaagtaa ttgaaaattt ttcaatagaa ataagtaatt aaatggtaat
     1921 acatataaat ttatatacat attataagtc aaaaatcata ttaaataaat ttattaattt
     1981 attaattaat taattaattg tctgattcaa ctagtataag attattgtta aattcaaatg
     2041 caaggctctt ttgtttcaaa ttgatcctta cttcattggc aaattcaatc atattctctg
     2101 tgtttacatc gttagtatga atttctactc gtatatattt agtaggttca aattttcctt
     2161 cgaaataacc atcaaactca aaaattgata atgctccata ttttaaggtt atatctttta
     2221 ttatgtcatt ataaccttcc actctcggta aatagaattt tattgaatca ttgaaggtct
     2281 ttatcttata aattttttca tatctagtat gattttttgt tgatgtatca tcagatctat
     2341 tcattaagat gtttataaat attagttaag tatatttatt taattgtaat ttagagtata
     2401 ataaaaatag tgcttaaata ctcaaaaatg attagataag atatattttg acgaataaac
     2461 ctacaaaaaa taacatatca tcatcccacc ggaaaaatga gaaaaataaa gccaaagaaa
     2521 tactaaagtc aagagaatta gaccaactag aagaattgag cattagaatt gatacattag
     2581 tggaattatt gtctgataaa ggaatcataa ataaaaaagg ttatataaat aatgttatga
     2641 tgcgcataca tgaaacatct aaggccaaat cttttgatga gtgtgatgaa atataaatat
     2701 ataatcagcc ttaattattc tttaactaat tttgagtatt attattaatt actctggatg
     2761 ttagatagag ttacaataga aattataaag tcaaataata atggagcata attcatttga
     2821 attaaatgaa ataaataaat atcaatcata taaaatatat ttaataatat ctttatcttt
     2881 gattaatggt aatcatttgg attaaaaatt gtattattat atagtaattc ctttaataat
     2941 attctaatta atatttaaaa taagcagtaa taatcatata tattatgata gaagaactga
     3001 aactaaatta ttaacttatc attaatattt ctcttaactc taaactaata acaattaatt
     3061 aaaattagat ttttcttttt tttaaatatt tatgaatcta caaaatcttc aactattaat
     3121 gtgtcaaaat ctgtccaaat attaggtcca gtaaatatga agatttgaaa atgtaaatgt
     3181 ggaatatatg tataaccagt cagacctact tccgcaattt tttgtccttg ttttaccttt
     3241 tgtccttctt ttacattaga actattaaat ttgagatgat catatcttga gatttcatta
     3301 ttcttatgca taattgaaat aaaattagaa aagttccaat acgcagggct agcccctcct
     3361 atttttgagt catctttaac aaatgttatt attccatctg ttgctgcgag tacaggggta
     3421 ttttctggaa ctataaagtc tattgcattt cttaactttc ctatatgagc aggtgatgta
     3481 tttctatcaa ttcgttcaat agaattcttt ggtacaggaa gctgatattt atttaaagca
     3541 gcggttctat tatctaggtt attcatcttt cactgagatt attacataaa atattattta
     3601 ataattcata acaaatattt tatattctac atattgaatt aacattgctg aaaactgaaa
     3661 acgcaaaata ctataattat tttatactat ctctttgcat ctaattaaca aggttaatga
     3721 aatctatatt taaatttctt ttaggtaacc cgcattccta tacaatattc taacgcattc
     3781 agtatatgtt ctatacctaa attttatttc cttaacaggc atatcttcag ttaaatgttc
     3841 ttttgctgta tcaatagaat attttttagc aatatcatct aaaattgtat ttatagttga
     3901 ctttatatat gattccacta tgagatttga cacattatat caagcactgt atagtatata
     3961 attactgtat ttcttttagt aatatcgtat gttaattcat agaatcaatt ttataaatta
     4021 acttattttt attattctat gctataataa tgtagtgtaa ttttgacgaa g
//
LOCUS       DASVEV010000263         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1694383, whole genome shotgun sequence.
ACCESSION   DASVEV010000263 DASVEV010000000
VERSION     DASVEV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694383"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF222_11540"
     CDS             complement(<1..550)
                     /locus_tag="VF222_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7143098.1"
                     /translation="MHESSKEPSDDTAYDDTNSPSSQHSTNEIENSLIQNKNSLASSS
                     NLFHEGIIAGKRIAKISVVTLLAIGVVEIVTGYLSGSVVATADGIDSVSDAMISFIVL
                     LGLRIAHKPADKKFHFGYHKVESFAALMAAIGMVIIGIVIFYNSYQSLIHPHEIKEPI
                     LVMIVLAGASALSLHRAFQMRKI"
     gene            1524..2147
                     /locus_tag="VF222_11545"
     CDS             1524..2147
                     /locus_tag="VF222_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7143099.1"
                     /translation="MDQTFAKNVSYCGILIIIALLISSLTMLSLQIFIINSIYGQNYN
                     ENPQIVKLKINLSVTGTSSDDKITTGNGDDTIVGGKGNDRVDGKGGSDNIDGEVGNDI
                     LFGGTGNNKLHGGKGNDTLNGERGNDELNGGKGNDEINGGRGDDILKGGQGDDILYSG
                     EGNDELDGGKGEDILNGGWGADIFICDKLDQLLDFNSTNGDKKIGKC"
     gene            complement(2642..>2822)
                     /locus_tag="VF222_11550"
     CDS             complement(2642..>2822)
                     /locus_tag="VF222_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143100.1"
                     /translation="GNGLYIDNQSNQNNVNFNNVLLNTIDVNNANGLPVNTNGNTYDQ
                     NNCMTSNPSGICIGK"
BASE COUNT         1083 a          369 c          420 g          950 t
ORIGIN      
        1 caatctttcg catttgaaat gctctgtgta atgataatgc actagcacca gccaacacaa
       61 tcattactaa gattggttct tttatctcgt gaggatgtat taatgattga tatgaattat
      121 aaaaaattac tattcctatt attaccattc ctatagcagc catcaaggca gcaaaacttt
      181 ccaccttgtg gtatccaaaa tggaattttt tgtcagctgg tttatgagct attctaagtc
      241 caagtagcac aataaatgag atcatagcat cagatacaga gtcgattccg tcagcagttg
      301 ctactacact accacttaga tatcctgtta ctatctcaac aacgccaatt gctaaaaggg
      361 taactacaga tatttttgct attcttttgc ctgcgataat tccttcgtga aatagattag
      421 atgaggacgc caatgagttt ttgttttgta ttaatgaatt ttcaatttca tttgtagaat
      481 gctgcgaaga tggactatta gtatcatcat aagcagtatc atcagaaggt tctttagatg
      541 attcatgcaa ttttagaatt taacatttat aatcattcgg gatatttaaa gtagatcttc
      601 ccaatctttc tttatcccta tatactatca gtcgaggtta tacttaaaaa taaatattca
      661 ttctctaaca aatttgatat tgactaattc ttaaaattat aattattcta aattctttct
      721 atgataatcc ttctttacaa gtacccatat taaaatagga aaaattatcc caactaccag
      781 tattacttct cttgttaaaa aaatgaaaaa acctaatact ataagccaca caatacatgt
      841 tgtctctatc aaatcatacg tgttaatcaa gatattataa ccattacgtt cagtatatat
      901 ttaagctaaa tatttcattg tattgtataa tatactacag tattaaaaag caatacaaag
      961 acggattaac ctaagaataa aagaatttaa aaagtatatt caagatccgg tttcaatagt
     1021 ggttccgaaa gaatatacgg tatttaggaa gctaatgaat ttgaaagtat gttaaaagaa
     1081 caaaaaaagt gaatatataa ctgtgggaac aatcctgcaa atctagttga tttcgacgga
     1141 actggacatt ataatctcat gaattcatat aaattaaaac aatagctcca attacattat
     1201 tatttcctaa ctcagacaaa ttaggatcta actataaaaa tgatatgttt gtaggagttg
     1261 tagacaggag aagaattttt cattttgacc taaatgataa tagaaatgat tcaacaatca
     1321 ctaacaacaa agtaataata atattattat taaaaccgac taacatttag cacaatacaa
     1381 aataataaaa caataacaca tagattaact ttttaatatt ataaaaaatt tataaaaaat
     1441 attcaattac ataacaatat gtaataaaaa tttcacataa aatacccaat atttttaaat
     1501 cgaaataaac ttattattta atagtggatc aaacatttgc taaaaatgtt tcttattgtg
     1561 gtatattgat aataatagca ttattaatta gttctttaac tatgttatct ttacaaattt
     1621 ttattataaa ttcaatttat ggacagaact ataatgagaa tccacaaata gttaaactaa
     1681 aaataaatct ttcagtaaca ggaacaagta gtgatgataa aatcacaact ggaaatggtg
     1741 atgacacgat agttggagga aaaggaaatg atagagttga tggaaaaggg ggcagtgata
     1801 acattgatgg tgaagtgggt aatgacatat tatttggagg aacaggaaat aacaaactac
     1861 acgggggaaa aggaaacgat acattgaatg gtgaaagagg taatgatgaa ttgaatggag
     1921 ggaaaggtaa tgacgaaata aacggtggga gaggtgatga tatactgaaa ggtggacaag
     1981 gtgatgatat actatattct ggagaaggca acgatgaact tgatggagga aaaggtgaag
     2041 atatactaaa tggaggatgg ggagcagata tattcatctg tgataaatta gatcaactat
     2101 tagattttaa ttctacaaat ggggacaaaa aaataggtaa atgttgaacc ggagaataaa
     2161 ataatagtat tggaacttct tatgtcttct aacgaacgaa atacaaaaac atgttttaat
     2221 ttacaacaat gggttaaaca ccttttaatg aattatctta ataaaaaatc cagtaaacat
     2281 ctaataaatt ttagtatatt ctaaatgttt tattactcct ttagattata ctaaatctgc
     2341 aagaatccca ttattacctc tacattaaaa gaaaataata ataattcact agatatcatc
     2401 atactagaga aaatcctaat agagcttatt gtgactttct tgatatttgt tcaacaaatt
     2461 attgtggtta tattgttctt attacttttt ctcttctatt actgtattag atttcaggtt
     2521 atatcgtaag aaagaataat agatgtataa attagttaat tattgttgat gtaaaatata
     2581 atatagttta tgtcatttta tattataaaa taaaaaaaat agataaaatt ttttttgttt
     2641 attacttacc aatacatata ccgcttggat tagaggtcat gcaattgttt tgatcatatg
     2701 tattgccatt ggtgtttact ggtaacccat tagcattgtt tacatcgatt gtattcaaaa
     2761 gaacattgtt aaagttaaca ttgttttggt tactttgatt gtctatataa agaccatttc
     2821 ca
//
LOCUS       DASVEV010000264         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_725081, whole genome shotgun sequence.
ACCESSION   DASVEV010000264 DASVEV010000000
VERSION     DASVEV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725081"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF222_11555"
     CDS             <1..534
                     /locus_tag="VF222_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX7143101.1"
                     /translation="VHDIENALLGTSLGMNIDDGTLAVRGHRNHMQSINEVFKAGSIK
                     NMVEQGILKKGIMYECIKNDIPFVLSGSIRDDGPLPDVVIDIVEAQRKYKEVLKDAKM
                     VIMLSSMLHSIAVGNMLPSYVKVIAVDINQSVVTKLLDRGTTQAIGIVTDVGVFLPMV
                     LQYLQEINHQEKNSNSE"
     gene            complement(1043..2485)
                     /gene="gatB"
                     /locus_tag="VF222_11560"
     CDS             complement(1043..2485)
                     /gene="gatB"
                     /locus_tag="VF222_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7143102.1"
                     /translation="MTSSSSLPVKIGLEIHCQLTQLNTKLFCSCYCNYREKEINSNIC
                     PICLGLPGSLPILNKKAVEFAIMISKALDCKIPELTVFSRKNYFYPDLPKNFQITQYD
                     SYGTSTSIGKEGSVKYGENNKNARIRRIQLEEDPGRLVYENGRMQALIDYNRAGVALV
                     EIVTEPDFTDPKDVRTFLNKMTSILEHLNVCNTNLEGSLRCDANISIGNGKKVEIKNV
                     GSFSDIEKALTYEITRQKTMTVHDIEIKAETRHWDDKRKITKQARSKEEEEDYRYFPE
                     PDIPKVLLENLSLIKTPELPDERKFRFINDYGLSEHVSQVLIDNKDLADLFESAIKIY
                     HSPKSISNWIVSDILAFMEYDSSGKKTITDEMKINSKHIAEIAKLVDDNTINRSTAKS
                     IISRIIKTGELPSDIIKKEKQMTTIINDEKILLESIDRIFKEEKKATLDAKQNPTAIN
                     FLLGKIMRLTKGRADPKITTILLKKRLDEV"
     gene            complement(2488..3945)
                     /gene="gatA"
                     /locus_tag="VF222_11565"
     CDS             complement(2488..3945)
                     /gene="gatA"
                     /locus_tag="VF222_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7143103.1"
                     /translation="MSNLFALNIEQVANGIKNGEFTCEEYVSQIIERIKKVDKKINSF
                     ITINDDALVQAQQIDRKIRSGSTVGSLAGIVVGVKDNISTAGLKTTCASKMLSDYVPP
                     YDATVIKRLKDEGAIIIGKLNLDEFAMGSTTEWSSYGLTRNPWNLDYVCGGSSGGSAA
                     SVAAQLCTASLGSDTGGSVRCPSSFCSVVGFKPTYGLVSRYGLVSYSNSLEQIGPIGR
                     TVSDVKKIMEVISGKDTNDNTTIDTKNNINNNHYDLEQKNNQKIGLLKELINGADEPV
                     YQCIYSAIDKFNELGFTSEEPSLKSSEYALASYYTIATAEASSNLARFDNIRYGFELN
                     PDDYEWNIYFSKARGNFGDEVKRRIIVGSFVLSSGYYGKYYLKAQRVKDLIKHELKNL
                     FKEYDLLISPTVPVLPFKIGEKIDDPLKMYLIDINTVLANLAGIPAISIPIGFKNGLP
                     IGLQIMANEFQEKKLLNIAGVLEKSININTSYPDL"
     gene            complement(3947..4225)
                     /gene="gatC"
                     /locus_tag="VF222_11570"
     CDS             complement(3947..4225)
                     /gene="gatC"
                     /locus_tag="VF222_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7143104.1"
                     /translation="MITKDQLKHLSRLSKIDLTENEFETYTKQIEKIIKYLDKLDEIS
                     FEENNPSLLLSFKKLSELREDTIIEPTLNIDQIAKNLKDKFVRGPVMS"
     gene            complement(4265..5614)
                     /gene="aspS"
                     /locus_tag="VF222_11575"
     CDS             complement(4265..5614)
                     /gene="aspS"
                     /locus_tag="VF222_11575"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX7143105.1"
                     /translation="MNSNTLREEELGNWRKTHYSSEINQSFEGKTVTLMGWISSIRDH
                     GNIQFIMLRDKDGDTQVIAKKGQCPDLTYEQVLQLKEHTSLGVHGKVISQRKAPNGIE
                     IIPLELKVFSIPIQAPPFMIQNRNPAGIDTRLDYRAIDLRRKYLQDIFIIRNNILKYT
                     RTFLSDERFIEVNTPKIIATASEGGTALFPIFYYEREAFLAQSPQIYKEQLTMAFEKV
                     FEIGPIFRAEPSRTNRHLSEATSIDIESAFVDYNDIMVLLEKMIQFIIKSLSEDDNIK
                     DSPELDIDQKINDNNKSFPRLKYSELTEKLRNIGEKVRWGDDLSPQLIKKLDDDLLNN
                     NFYFITDWPTAAKPFYVKENQDDSKICESFDLMYGSLEISSGSTRISNKEILINRMKK
                     KGLNLNSFDYHLRIFSYGVPPHAGCGVGLERLLMTLTKIDNIRDAILYPRDIDRLAP"
BASE COUNT         1734 a          930 c          801 g         2240 t
ORIGIN      
        1 gttcatgata ttgaaaatgc acttttagga acttctttag gtatgaatat agatgatgga
       61 actttggctg tgcgtggtca taggaatcat atgcaatcta ttaatgaagt ttttaaagct
      121 ggttccatca aaaatatggt agaacaaggt atattaaaaa aaggaattat gtatgaatgt
      181 attaaaaacg atataccctt tgtattatct ggttcaatta gagacgatgg tccacttcca
      241 gacgtggtta tagatattgt agaagcacaa agaaagtata aagaagtttt aaaagatgcc
      301 aaaatggtga taatgctttc ttcaatgtta cattcaattg ctgtaggcaa tatgttgcct
      361 tcttatgtaa aagtgattgc agtagatatt aaccagtcag tagttaccaa attattggat
      421 agaggaacta cccaagcaat aggaattgtg actgatgtag gtgtatttct tccaatggtt
      481 ttgcaatatt tacaagaaat caatcatcaa gaaaaaaata gcaatagcga ataataagta
      541 ttatatgaaa aatattgtgg attgttataa tgcctactgg gatctattag tttaatgtac
      601 atctatccat aaccatcatt ttaccttctt tcaattcaat aataaaatag ttcttcttat
      661 attttgtatc attgtacatg gaaacaaaga gagaatgaat tgtatgcagt tatgtatata
      721 agataggtta cattgctttt ggatacatag atggtagtca tcatcatcaa aatcttccgt
      781 tctatctttg aaatattgtt tgattagttt ctataaaaat atacataaat tttatggtag
      841 tgataaatta tgtaataatc gcacaaaaaa attggaattc gtgtgagcga caaattaaaa
      901 tctaaacaat tttttaggtt ttaaaacaac ttctttttct atatttttat tcgtttttga
      961 gactttcaaa aatatatgga tcaactttac atgaattatt tgtttggtat aattttaata
     1021 ctattatttt tttatttgat tcttagactt catccaacct tttctttaaa agtattgtag
     1081 ttatttttgg atcagccctt ccctttgtca gtctcattat cttgccaagc aagaagttta
     1141 ttgcggtagg attttgttta gcatctaacg ttgccttttt ctcttctttg aatatcctat
     1201 ctattgattc tagtaaaatt ttctcatcat tgattattgt ggtcatctgt ttttcttttt
     1261 ttatgatatc agatggtaat tctcctgttt taataattcg ggagattatg gattttgctg
     1321 tagacctatt tattgtatta tcatcaacta gctttgcaat ctctgcaatg tgttttgaat
     1381 taattttcat ctcatcagtt attgtttttt tacctgatga atcatattcc atgaatgcaa
     1441 gaatatcact tactatccaa tttgatatag attttggaga atgatatatt tttattgctg
     1501 attcaaatag gtcagctaaa tctttattgt caatgagtac ttgtgataca tgttcagaaa
     1561 gtccataatc atttataaat ctgaattttc tttcatctgg aagctcaggt gttttaatta
     1621 aagataaatt ttctagcaga acttttggaa tatctggttc aggaaaatat ctatagtctt
     1681 cttcttcttc ttttgatctt gcttgctttg taatttttct tttatcatcc caatgtcttg
     1741 tttcagcttt aatttctata tcatgaacgg tcattgtttt ttgtcgtgtt atctcatatg
     1801 tcaatgcttt ttctatgtca ctaaaagaac ctacattttt gatttctact ttttttccat
     1861 tacctatcga aatatttgca tcacacctga gagaaccttc taaattggta ttacatacat
     1921 tcaaatgttc tagtatagaa gtcattttat ttaaaaatgt tcttacatct ttgggatcag
     1981 taaaatcagg ttcagttacg atttctacta atgctacacc tgctctatta tagtcaatta
     2041 aggcttgcat tcttccattt tcataaacca gtcttcctgg atcttcttcc agctgaatcc
     2101 ttcttattct ggcatttttg ttattttcgc catactttac agatccttct tttcctatgc
     2161 ttgtactggt accataagag tcatattgtg taatctggaa attttttggc aaatctggat
     2221 aaaaataatt ttttcttgaa aaaactgtta attctggaat tttacaatcc aaagcttttg
     2281 aaatcataat tgcaaattct acagcttttt tattcaaaat tggcaaggat ccaggcaatc
     2341 caagacaaat tggacatatg ttagaattga tttctttttc tctataatta caataacatg
     2401 aacaaaataa tttagtatta agttgagtta gctgacaatg tatttcaaga ccaattttaa
     2461 ccggtaatga tgatgatgat gtcattatta tagatctgga tatgacgtat taatattaat
     2521 tgatttttca agtactcctg ctatatttag taattttttc tcttgaaatt cattagccat
     2581 gatttggagt ccgatcggta gaccattttt gaatcctata ggaattgaaa ttgcaggaat
     2641 gcctgccaaa ttagctaaaa ctgtgttaat atctattaga tacattttta atggatcgtc
     2701 gattttttct ccaattttaa aaggaagaac cggaactgtt ggggatatta gtaaatcata
     2761 ttccttaaaa agatttttta gctcatgttt aattaaatcc tttactcttt gtgctttaag
     2821 ataatatttt ccataatatc ctgaagataa tacaaaagat cctactatta ttcttctttt
     2881 gacttcatct ccaaaatttc ctcttgcttt cgagaaatat atattccatt cgtaatcgtc
     2941 tggatttaat tcaaatccat atcttatatt atcaaatctt gctaaattgc tgcttgcttc
     3001 agctgtagca attgtataat atgatgcaag agcatattct gatgatttta atgatggttc
     3061 ctcacttgta aatcctaact cgttaaattt atctattgca gaatatatgc attgatatac
     3121 aggttcatca gctccattta ttaattcttt taataaacca atcttttggt tatttttttg
     3181 ttcgagatca taatgattat tgttaatatt atttttcgtg tctattgttg tattgtcatt
     3241 agtatctttt ccagagataa cttccataat ttttttgacg tctgatacag tccttcctat
     3301 aggaccaatt tgttctaaac tattagaata tgaaacaagt ccatatctac ttactaatcc
     3361 gtatgttggt ttaaatccta ccacagaaca aaaactagat ggacaacgaa cagagccgcc
     3421 agtatcagac cctaatgatg ctgtacataa ttgtgcagca acgctagctg cactgccacc
     3481 tgaagaccct ccacacacat aatccaagtt ccacgggttt cttgtaagtc cataactgct
     3541 ccattctgta gttgatccca ttgcaaattc atcaaggttt aattttccta ttattattgc
     3601 accttcatct tttaatctct ttatgacggt ggcatcgtat ggtggaacat agtcggatag
     3661 cattttggaa gcacaagttg tttttaatcc cgctgtactg atattatctt ttactcctac
     3721 tacaatgcca gctaaagaac caactgtcga acctgatctt attttcctgt ctatctgttg
     3781 tgcctgaaca agtgcatcgt catttattgt tatgaatgaa tttatctttt tatcaacttt
     3841 ttttattctt tctataattt gggatacata ttcttcacaa gtaaattctc catttttaat
     3901 accatttgca acttgttcaa tatttagagc aaaaagatta gacatattaa ctcataactg
     3961 gacctcttac aaacttgtct tttaggtttt tagctatttg gtcaatatta agagttggct
     4021 ctataatcgt gtcttctctt aattctgaca attttttgaa agataataat aatgacgggt
     4081 tgttctcttc aaatgaaatt tcatctaatt tatccaaata ttttattatc ttttcaattt
     4141 gtttagtata agtttcaaat tcattttcag ttaaatctat ctttgaaagt ctactcaaat
     4201 gttttagttg atcttttgta atcatgtttg atttatcact tctcttcccc ttctttgttt
     4261 tcttctatgg cgcaagtcta tcaatatctc ttggatataa tatagcatca cgaatattgt
     4321 ctatttttgt gagagtcatt aaaagccgtt ccaatcctac tccacaccca gcatgtggag
     4381 gcactccata actaaatatt ctcaaatggt agtcaaatga attcaaattc agtccttttt
     4441 tcttcattct atttataaga atttctttat tactaattct tgtgcttcca gatgatattt
     4501 ctaatgatcc atacattaaa tcaaaagatt cacatatctt tgaatcgtct tgattctctt
     4561 tcacataaaa tggtttagct gctgtgggcc aatctgttat gaaataaaaa ttattgttta
     4621 acagatcgtc atcaagtttt ttaattagct gcggagatag atcatctccc catcttacct
     4681 tttctccaat atttctcaat ttttcagtta gttctgaata ttttaacctt ggaaatgatt
     4741 tattgttatc attaattttt tgatcaatgt ctaattcagg actatcttta atattatcgt
     4801 cttcagacaa tgactttata ataaattgaa tcattttctc taataatacc ataatatcat
     4861 tatagtcaac aaaagcagat tcaatatcta tcgatgtagc ttctgataag tgtctattag
     4921 tacgagaagg ttcagctctg aaaattggtc ctatctcaaa gactttttca aaagccattg
     4981 taagttgttc tttgtatatc tgaggactct gagccaaaaa tgcctctctc tcgtaataga
     5041 atattgggaa aagggcagtt cctccctctg aagctgtcgc tattatctta ggtgtattga
     5101 cttctataaa tctctcatcg gaaagaaaag tgcgagtata ttttaaaatg ttattacgga
     5161 tgataaaaat atcttgcaaa tactttcttc tcaaatctat tgctctatag tctaatcttg
     5221 tatctattcc tgcaggattt ctattctgta tcataaatgg tggagcctga ataggaattg
     5281 aaaaaacctt tagttctaat ggaattatct caattccatt aggtgctttt ctttgagaaa
     5341 taacctttcc atggactcct aaagatgtat gttccttcaa ttgcaaaact tgttcatatg
     5401 tcaaatctgg acattgacct tttttagcaa ttacttgggt atctccatct ttatctctta
     5461 acataataaa ttgaatattt ccatggtccc taatgctgga aatccatccc attagtgtaa
     5521 ccgtttttcc ttcaaaagac tgattgatct ctgaggaata atgagttttt ctccaatttc
     5581 ctaattcttc ttctcttaat gtattactat tcaattttaa aacctaaata acatactaac
     5641 aattatacta gactaattta tccataacgt tttaaatata acacgactac gaacaaaaat
     5701 aaggt
//
LOCUS       DASVEV010000265        16355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1696845, whole genome shotgun sequence.
ACCESSION   DASVEV010000265 DASVEV010000000
VERSION     DASVEV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696845"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..1526)
                     /locus_tag="VF222_11580"
     CDS             complement(552..1526)
                     /locus_tag="VF222_11580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7143106.1"
                     /translation="MTNDYEIIVVGAGPAGLAAGIYIARQRISCLVISKDLGGQLNLI
                     PRLENYPGTIMSSGPLLARTLENQYLTFKGEMIYDTIERIDESENGLKVKTNRSEYSC
                     KSIVLAPGKVPNNLGIANENVFANKGVHYCTKCDAPFYQDRITATVGVGGYLLESGIL
                     LARMASKVYLIYRGGALGGDKEIISSIEKKDNVELVPNTIIKEIHGNNTLQKIVIKDN
                     QGLEKTINIDGLFIEMGSKINLDFVRHLVKVNSKGEIEVTEGGKTAHPAIFAAGDATN
                     ILYKQIVAACGDGAAAGLSAFNYIEKLKGKPGIRSDWKKTIGDTVYHY"
     gene            complement(1527..1757)
                     /locus_tag="VF222_11585"
     CDS             complement(1527..1757)
                     /locus_tag="VF222_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7143107.1"
                     /translation="MNEAYILLNIDYKQQQSIIETAKKISCVKEVKSVYGIYDILVIL
                     ESSDIQQIKDSIDNDLYKMEGVNNLTSLISVG"
     gene            complement(2633..3760)
                     /locus_tag="VF222_11590"
     CDS             complement(2633..3760)
                     /locus_tag="VF222_11590"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7143108.1"
                     /translation="MNDKPVSAQVFEFQEDDYFDVDGENPGSSTNTNEDAYSNTNDDE
                     NANTNDEEEDDSSDKTSSKKQKKGYENNDEPNYGMSEGDVQKYYVQKYDLNGKFLGGF
                     GSRGNGDGQFLHAHNIAVDSNGNIYVTDEQRMDVQKFDSKGNFLLSWGAPADQNLFSH
                     KMEDVNVDKEGNIWVVNWGPGKLMKFDPNGKLLMTIGKKGSGPGEFNRLWGVAFDSDN
                     NIYTSEKYAYRIQKFDPAGNFIKQFGEYGEGNGQFRHLHAVSIDSKDNVYVNDEDNGA
                     VTKFTKDGKFITNWGSVGKNEGQFDEPHGIDFDSQGNVYVVDTQNNRIQVFTPDGQYL
                     KSIGKPGSAPGEFLLIHDIDIDDNDIIHVLDSRDAHPNKIN"
     gene            4521..4697
                     /locus_tag="VF222_11595"
     CDS             4521..4697
                     /locus_tag="VF222_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7143109.1"
                     /translation="MWNNKGTTLLNLERYEDAIYCYDIVISLNSHLANTYYNKGVALA
                     NIERYEDAINPMTM"
     gene            complement(5151..5438)
                     /locus_tag="VF222_11600"
     CDS             complement(5151..5438)
                     /locus_tag="VF222_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7143110.1"
                     /translation="MSSNKNKIKCLHILVKKNSEALAVVDRLKNGESFTNLAKELSID
                     KASGKRGGDLGFFRKGMMVKPFEDVAFKLEKGQISEPVKTEFGYHIIKRIS"
     gene            5559..5957
                     /locus_tag="VF222_11605"
     CDS             5559..5957
                     /locus_tag="VF222_11605"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7143111.1"
                     /translation="MILFSQKESKFIIDNEICRIATAKKNIPHVVPVCYIYKDGDIFF
                     ATDYITQTFHNLKNNIRISLIIDIYDRKNGNKGILLNGIASVIDRGKEFANLFKVFYD
                     KFDWVKNDPWGEEEAPFIKILLTKKVSWGI"
     gene            7721..8278
                     /locus_tag="VF222_11610"
     CDS             7721..8278
                     /locus_tag="VF222_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143112.1"
                     /translation="MTSLGFFLSITLFTLLSLTSDYNILKDSYEQMLGGHGHNLPPSI
                     VGDREIFLNFTNPKIESAAEKGIINFALVDNKTGNNIPHVTYIVGIYNQENKNMFTAN
                     LHGHNGEIKLEFHNKGHEGYNINANYDSLSASYVSDFGSPIKIDGSIFSDPGKYRVVS
                     EITGIDFDNTFLPEPLKYEYNINVR"
     gene            8388..9179
                     /locus_tag="VF222_11615"
     CDS             8388..9179
                     /locus_tag="VF222_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7143113.1"
                     /translation="MKFKDQIIAITGASSGIGKQAAIDFSYNGAKTIILISRNIMRLN
                     QVKKKLNPDCTVEIYPCDISNKKMVYEVGQNIMKKIGAIDILVNNAGIGIYGKVIDQT
                     IEDIEKVTSTNYLGMVYFTKSFLPAMIKNKRGHIVNVASLAASFGIPCMAAYCGSKFA
                     MLGFSESLSYELKNTNVGVTVVSPIAVKTNFFDNESFKGKMPHKLGYILDPKTVSKAI
                     LKAACSKRIEIVVPFFVRSAVWLKHTFPYLINPIISSSFKDQNYN"
     gene            complement(9315..9476)
                     /locus_tag="VF222_11620"
     CDS             complement(9315..9476)
                     /locus_tag="VF222_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143114.1"
                     /translation="MIDRNYQKKKILVEKFIKKNKKTDHSVILNEVNIDYDTLMLIIS
                     DLRREGRIK"
     gene            9564..12146
                     /locus_tag="VF222_11625"
     CDS             9564..12146
                     /locus_tag="VF222_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7143115.1"
                     /translation="MKKYRCPNCTSLNSLRVNRIFDGRLLFTCEKCHICSVLPFYNNL
                     DETYLEFLDRFDNGYVTIFEDLKVILEKEKLVRSKTEIEKMIKKSKINNNLINEILYS
                     DLDYVVDVIKLEESKIALGKELDELPLDDTILNQLKSRNISTLYKFQEEAIFNILQEK
                     NTVIIAPTASGKTEAFSLPILQKIYDLKNQLNIKNVTFSYNQNEFGTKQIKAVFIYPT
                     KALARDQLSKIMEYSKNMNIMVKIFDGDLNANEKQNFYEFPPDIVITNFDSLHYHLLN
                     NTKLIKLLNTLEYLVVDEVHTYTGIFGSNVHYIIKRLERIINNKKLLLIACSATLRNV
                     KEFCDLLFSRKMEIVNGIGKKGHTNFAILYPSLRSNKSLVIELIKKITKNHKTLVFSN
                     SHLSAELIAFTSSKQGIKIGVHRAGLPPKNRKVVENSFKNGIIDVISSTPTLELGIDI
                     GDVDAVISNLVPINRLVQRLGRAARKGQEGYAFLTLGNDPISQYYKNHPADYFVDEEI
                     PYIDPFNPIIMENQILAMCFDRPISKLESKNYLLTLSKLERKGLVIFSNGKYLPNREK
                     QNILKNYSIRGIGNVVDILFNGKIIGNRNLPQALEELHDDAIYFLSGRRYKVKKLILD
                     EESERNFADIDKIPMNYPYYTKSIMEEYPEVIEIFEDKEIFGIKLRYCSLNIFKKVLG
                     YSNIEIGKEINQGKKVFFSEPITYNYTTKGFIFRVPSPIEILKMTSEKEKVEVSGYHA
                     TEHVLIEGSSMLTGGASQDIGGISLGDTGMIVIHDGSIGGNGASKSLFDKFELAVKRA
                     HSILIECTCENDDGCPRCTYSYRCGNNNEYLHKKAAIEVLTRILSMEKTDLLDFNRSQ
                     QTLI"
     gene            complement(12215..12433)
                     /locus_tag="VF222_11630"
     CDS             complement(12215..12433)
                     /locus_tag="VF222_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143116.1"
                     /translation="MGVILWFIAIVLFLAVIGLGWETFFTGVLKGVDKIGITPVIKNI
                     TDSTKDSLQGIISESSKNTLDNNLKFNS"
     gene            12571..12963
                     /locus_tag="VF222_11635"
     CDS             12571..12963
                     /locus_tag="VF222_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143117.1"
                     /translation="MNLLKISAITLKVKSMRNEWQFYSKIPGLSLCYGGPDSSFTSFE
                     MTEEFSNSKTYINLEFTKGLKCDTNFGRIIIYSKNVDNLYDYFKQDQDICRLITIKNK
                     PVDGLWGERYFHILDPEKYELSFAQVIS"
     gene            complement(13040..14725)
                     /locus_tag="VF222_11640"
     CDS             complement(13040..14725)
                     /locus_tag="VF222_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143118.1"
                     /translation="MNISSKNNEYYAFLISSFLTLTIFISSSNLNSNFSPTFLINEFM
                     YSDILTQAFGDQQEFSKQQEFSKQQAIEKFKNQFCGINSKPNSNQYIKEIKLNNECEM
                     PLAITYDDNVEGLWYISTKQGSLILYQPETSIFTKYDIPTWPSRDNPIDSSQVWDLEL
                     DPSNENLWFTDEKQNLIWKFSKQNKEFEHFKIPETNLSFGTVYPVSIDFDNNGHIFFA
                     GIRSQSLWMGNLSLMKSSTSDGIAKIPLPVEDFKDFDPSVISTGSIVVDKKNNNVWVS
                     VLAFGYKGQIYKYDITNKSFKKYDLGDLPSPVGITIDETGNIWVSDHGTSIFVKLNPI
                     NSSLTKFITSTASKKIFAGEEVNGQAYTLPYWMKKGQNNSIIFNEHTGNKIGKFFPDE
                     LKLVEYWIPSQNKLFGQCKPDLPENSCGIGNVLQLTDETYNGSIWFTEWSENKIGYVN
                     TSIPLPFSIKTENNEMTLTPGESKEIRFEINANNNSSLHPISSSTLTPSGDLGASYGI
                     FSENSIALKKDESKEISFVFTLSNDVAMGNYILMLGAEDDIISISKAILIKVI"
     gene            complement(14817..15917)
                     /locus_tag="VF222_11645"
     CDS             complement(14817..15917)
                     /locus_tag="VF222_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7143119.1"
                     /translation="MNVYLHYVSAEWKIPSNTEVTPNFTTYENNFLKIDYPKNWIFTE
                     TTDSVIFAPDRDSIDKIKINVSPVSSDSLSIKSVVDSTLDEFVRTLDDFELIESSQLS
                     NIKDNNHRLIYSYIDGNKNKIQNADVGIIQGANLYIISLTSNYTDYYRNLPIYEKMLT
                     SFNYYNEQGLLNQFSSYLLKPVADFVPILGNSSNITMVEFGDYQCTFCSKFHNETREQ
                     IIKNFVDSGKINFIFKDYIVNDIPTDKGSTMGAEASYCAGEQGKYWDYHTELYDNWKG
                     EGTGWITSDNLKQFAKNIQIPNIKQFSDCIESNKYSLIVQNNDNIARSMGLSGTPSFI
                     LIKDNNIQSIIPGALPYEIFEQTLNRLISSKF"
BASE COUNT         5989 a         2404 c         2231 g         5731 t
ORIGIN      
        1 taagaaaaga agtcttccaa taactttatt ctgccattct cgtattttct gaattcttaa
       61 ctcatctgta atggatttaa aaaaaggtct atttattttt tccttttgca taatatttat
      121 tgatctttat atgcaactgc gttattttaa actttatata tattaattat aaaataacca
      181 aaggagcaaa gcaatacaaa agaaaactaa atcgtctcaa agttaaatag aatatttcat
      241 attaatattc cttaacaatt caatgtccat catagcctgc tattacaata ccataaaata
      301 gtaactgttc tattccttgt gaaataggct atgacaatta aataaattat cttccattaa
      361 taaatattat atttaacaat ggtgtttcct tagacaaacc agtcattcca aagaaccggc
      421 atatcatagc ctcctgttac tatctcttcc gaggaggagt atcattttac tatgacaatt
      481 aaattgttat atagcagagt aagccaatca ttttttaacg acttaaattt attaatttat
      541 ctccgttgta tttagtagtg ataaacagta tccccgattg tctttttcca atctgaccta
      601 attccgggtt ttcctttcag tttctcaata taattaaaag cagaaagacc ggctgccgct
      661 ccatcaccac aagctgccac aatttgctta tataaaatat ttgttgcatc gccagctgca
      721 aagatcgctg gatgtgctgt ttttccccct tcagtcactt ctatttcacc ctttgaatta
      781 actttaacaa gatgtcttac aaaatccaag ttgatttttg atcccatttc aatgaataaa
      841 ccatcgatgt taattgtctt ttccaagccc tggttatctt taataacaat tttttgaaga
      901 gtattgttac catgaatctc tttaataata gtattaggaa ccaattcaac attgtctttt
      961 ttttctatag aagatattat ttctttgtct cctcctaaag cacctcctct gtagattaaa
     1021 taaacctttg atgccatcct agcgaggaga attccagatt caagaagata tcctcccact
     1081 cctacagtag ctgttattct atcttgataa aacggtgcat cacatttagt acaataatga
     1141 actcctttat ttgcaaatac attttcgttt gcaattccta agttatttgg tactttacct
     1201 ggtgccaaaa ctatagattt acagctatat tctgatctat tagttttaac ttttaatcca
     1261 ttttcagatt catctattcg ttcaattgta tcatatatca tttctccctt aaaagtaagg
     1321 tattggtttt ctaaagttct agcaagtaaa gggccactag acataattgt tccaggataa
     1381 ttttcaagac gtggaattag atttaattga ccgccaagat cttttgatat gactaaacat
     1441 gaaatccttt ggcgagctat gtaaatacct gctgccaatc ctgcaggtcc ggcaccaact
     1501 acaataattt catagtcgtt ggtcaattat cccaccgaaa ttaaagatgt aaggttattc
     1561 acaccttcca ttttgtaaag atcattatct atagaatcct tgatttgttg tatatcagat
     1621 gattctagaa ttactaaaat atcataaatc ccatatacag acttaacttc cttcacacat
     1681 gatatttttt ttgctgtttc aataatactt tgttgttgtt tataatcaat atttaataaa
     1741 atatatgctt cattcatgaa aatttcatcc tctatcttag tattatatta attttataaa
     1801 aattttcgta ttttatataa atcaataagg ttactaagat actagcggta tataaacatt
     1861 gtaatatatt taacaacaat agtaattata aagaattgtt ttagaaaata aagttcaaat
     1921 tagtcactaa atatctggta atattttata tattataatg attattggaa gaatatattt
     1981 taacaacaat atttaaacgg caaactttat tatctaagag atacatcata ttataattat
     2041 aatttataat aattctcata ctatcatgaa atcatatcat actaagacta aaaatatttt
     2101 gaatgaataa tattattatt tcataactcc atgatcttta atttgtcctt tttcacattt
     2161 tatatatctt atcaagacat gaattcctat cgaataaatt gtaaatatag taaatagtaa
     2221 aattattcaa ccaaatgaaa cattaccaaa gattaagggt atgatatgat atatcaaatc
     2281 aaactattaa tatatttaca aacaaaatta atcttatcaa atactcaatc ttcctgcaaa
     2341 acgcgtcagt atcgaatcag atgaactcaa tacatcaata ctctatagaa agattataaa
     2401 ttttgtaaat attgtaaggg tcaatagttt tttaaatgac tataattatc ttcaagaatt
     2461 aataatcctt cctaagagat agttatatga attacgattg taaagaacaa aaaagttctg
     2521 aatatataaa gtgatccact caaaatgggt agtaaaaaaa atatcctttt tacaataaaa
     2581 aaagaatttt attattaggt tctttaatca attaatactt agatcgcaga agtcagttta
     2641 ttttatttgg atgtgcgtct ctactatcta gcacatggat tatgtcatta tcatcaatgt
     2701 caatatcatg tatcagtaag aattcacctg gcgcagatcc aggctttcca atgctcttta
     2761 agtattgacc atcaggtgta aaaacttgaa tcctattgtt ttgggtatca actacgtata
     2821 catttccttg ggagtcaaaa tcgattccgt gaggttcatc aaattggcct tcatttttcc
     2881 caacagatcc ccaatttgta atgaatttcc catcttttgt aaattttgtg acagcaccgt
     2941 tatcttcatc atttacatac acattatctt tagaatcaat tgatactgcg tgtagatgtc
     3001 taaattgacc attgccttcc ccatactctc caaactgttt aataaaatta cctgcgggat
     3061 caaatttttg aatacgataa gcgtattttt cactagtata gatgttatta tcagaatcaa
     3121 aagctacacc ccacagtctg ttaaactctc caggaccact tccttttttc ccaatagtca
     3181 ttaataattt gccattaggg tcaaatttca ttagttttcc agggccccag ttgacgaccc
     3241 atatgtttcc ttccttgtca acatttacat cttccatttt gtgtgagaaa agattttggt
     3301 cagctggggc accccaagat aataagaaat tacctttact atcaaacttt tggacatcca
     3361 ttctctgttc atctgtcaca tagatatttc catttgaatc tactgctatg ttgtgagcat
     3421 gaaggaattg gccatcacca ttaccccggg atccgaaacc accaaggaat ttaccattta
     3481 gatcatattt ttgaacataa tatttctgaa cgtctccttc actcatgcca tagtttggtt
     3541 catcgttatt ttcataacct tttttctgtt ttttagaaga tgttttgtct gaagaatcat
     3601 cttcctcttc atcatttgta tttgcatttt catcgtcatt tgtatttgag tacgcatcct
     3661 cattagtatt tgttgaggat cctggatttt ctccatctac atcaaaataa tcatcttctt
     3721 gaaactcgaa cacttgagca gatacaggtt tatcattcat gttaaggtaa ctaaaaacta
     3781 aggtaaaagt aattaaaaat aaaaatgtaa agccaaccct ttttaatacc atcttcctat
     3841 aactagtcta tacataaata ttaagattat aataaaatta tctagaatat cattatagag
     3901 aacttgccac ctattataaa atatttacaa tatttcaaaa atagagcatt aaacgaattg
     3961 tgtctaccta aatttcacta tattattaat attaacattt cttattttat tttcttttga
     4021 taaataatac taattatttt gattcatata atataaatct tagataaact aaaaaatatt
     4081 gaaattttta ttctacgcaa acaaagaacc ttaaatataa aattaataga aaaaagaaat
     4141 gcatgaatgg aaaaaacact aattataaga agagatatta ttctaaaaat tgaaatttat
     4201 ctactttcag gacatattta atgtatatga agaagggttt atctttagaa caaaaaagaa
     4261 aatatacaga agctatttaa tgttttgaag atatcttaaa gaaagatgga gaacgcagat
     4321 gctcaatata agaagggact atctcttgag aattggaaag gtatagctat gcgttaattt
     4381 catacaacca agctataaag atcaaaacag attctcatga agtatattta gcgcaggaat
     4441 aacatgcgct aaaatgggaa aacatcagga accactaaaa tgtttgataa agtcattgag
     4501 ataaaggaag atgacattac gtgtggaata acaaagggac aacattattg aatctagaaa
     4561 gatatgagga tgctatttat tgttatgata ttgttatatc tcttaattct catttggcaa
     4621 atacatatta caacaaggga gtcgctttag ctaatatcga aaggtatgaa gatgcaataa
     4681 atcctatgac aatgtaatta tgattcgacc tgattatatt attcttatta ctgtaacgaa
     4741 aaggatcttg aaaggttaga aaaatattcg gatgccacta tatacatcat gaccacgaag
     4801 ggattacaat aagtggtaac acaaatcctt ttttaaattc taacttttat ctcatgaaaa
     4861 aataattgtt aaatttaaaa ttgacagtat ccaattttca aggtagtaat aattattatt
     4921 acagttttta actagttttt cacatactct ttccttgtga aatagaggct ctgcaaatca
     4981 caaaagaaaa ttttgataga gcattcgtct aaatatgtca aataatagcg tttatttaat
     5041 ttaccatcta gtctttttat taagtttcta ctcgaaatct tataaaaaat tcttacagat
     5101 tttccattat taaagagaca attctaataa agaggaagat aacgaattaa ttaactaatt
     5161 ctttttataa tatgataacc aaattctgtt tttacaggtt cagaaatttg ccctttttct
     5221 aatttaaaag caacatcttc aaatggctta accatcattc cttttctaaa aaatcctaag
     5281 tcgcctcctc gtttcccact tgctttatct attgataatt cttttgctaa atttgtaaaa
     5341 ctttcaccat tttttaatct atcaaccacc gccaatgcct cactattttt ttttacaaga
     5401 atatgcaaac atttaatttt atttttattg gatgacataa gggatctatt gtatcaacaa
     5461 tatttaaaat ataaataaat atttttataa ttataataaa ttatattgtt tgttaaacta
     5521 taaataaaaa taaaaattta tttaagttaa ctgatagtat gatactcttt tcacaaaagg
     5581 aatcgaaatt tattatagat aatgaaatat gcagaatagc aactgctaaa aaaaatattc
     5641 ctcatgtagt tccagtatgt tatatatata aagatggtga tatctttttt gctactgact
     5701 atatcactca aacgttccat aatttgaaga ataatattag aatatcacta ataattgata
     5761 tttatgatag aaaaaatgga aataagggaa ttcttcttaa tggaatagca agtgtaatag
     5821 atagaggcaa agaatttgca aatcttttta aggtatttta cgataaattt gactgggtta
     5881 aaaacgatcc atggggtgaa gaagaagcac cattcataaa aattctgtta actaaaaaag
     5941 taagctgggg aatatgacaa ttatacttta aaattagata tttagaaata acggactata
     6001 accatacaag aaagataata gtataatatt gtaaactatg attaataagt caagcaaatt
     6061 taataaaact aatagctcta ttatctataa tataactttc tactcaaata atcgtttttt
     6121 aactctaaat ggtttaaact tttaaatatc tttgttaaac tcttccaaga acggatttat
     6181 aatactttaa agaattgaat tagatttatt aataattaga atttagattt gtacaataat
     6241 gatgacggca tgaaaggttc ttcataaatc aattacaaat ctgaaaatgc taaattttta
     6301 tctattattt taaagttctt tttctctaat ttttcggcgc cgaaaaatta ctaaatttta
     6361 tctacaagtt atttactaag gaaattctca gatgagaaaa aattttaaat aattttatca
     6421 ctaattgata attgaggaac atatttgtaa cctcacccta tgtgatcaat cccagccaag
     6481 tcatcacaat ggggtaggcc aatgaaatgg cctaccccat ttcaaccttg atatctttaa
     6541 agatactaat tttcgagaat ttgagtcttt tcttgagcaa ttacattaaa caatcctcac
     6601 acttagtttt agcaaatgta gaaacaggtt gtgatgaata caatatacaa cttggaacct
     6661 gacaaccatg acaggttcta gcaatatcca accttgggga tttcaacatc tctatacttt
     6721 gttccaataa ttaatgtatt atttggtgaa ttatatttcc aatattcctt tttcatttat
     6781 aaaatacttc tccaaacatc accttcatcc taaaaacctc tttcatgatc aataatttgc
     6841 tgaactccag tattattatt ttacgatgaa tatgcctttt gtcataatca agtatgcttt
     6901 catatacagc aatcatagtt tttctatcag gcaataaaga ataatctaca tttgatctgt
     6961 cttctccaaa agtaaaagat actgtataag atccaaactc ttttgctttg tcaaagtaaa
     7021 tatgattaat aaattcctca gtaatatatt tttgttattc ccaacttaat aatggtaaac
     7081 gatgttttca ttcatttaag actatgaaat tttttattac tctattataa atgtccataa
     7141 aaagtcttcg taatttccaa catgattttt tcgtaaacaa aatctatact aaaagtaatg
     7201 aattcatata tttttattcc ctttaggtta atagtgtgtg taataataaa caatttaaaa
     7261 gtaaatttat agtcatattt aaattatgta aatatgttac cagttctcta aaatcagatg
     7321 catgtatgag ttcatgtcct tctacaataa accaaatata gtatttaggt gccttatcaa
     7381 aaagacattc ccattatgta gttgaaagat catttttaca agtttaattt gatccatttt
     7441 atcagaatca ccaaaatgac ataatggatg ataaaaattc ggtaaatagg ttaaataaat
     7501 acaggaatta agacttttta tcgataaaat aactaaatgt ccattcctaa aagcttaatt
     7561 gttctattat aatcaattac catataatta cattctatag aaaattataa atttttataa
     7621 tataaaaaga ataatagaag ttcaaacttt aaatattgaa tattgtaaaa tttttttaac
     7681 aagtatgaaa ctgaataaaa agttttctct agtatattac atgactagct taggattctt
     7741 tttatcaata acactattta ctttactatc gttaacatcc gattataata ttcttaaaga
     7801 ttcatatgaa caaatgctag gaggacatgg ccataatctt ccaccttcga ttgtaggaga
     7861 tagagagatc tttctaaatt tcactaaccc aaaaatagaa tcagccgcag aaaagggcat
     7921 aattaatttt gcattggttg ataataaaac aggaaataat attccccatg taacatatat
     7981 tgtaggcatt tataatcaag aaaataaaaa tatgtttacg gcaaaccttc acgggcataa
     8041 tggagaaatt aaattagaat ttcataataa agggcatgaa ggttataata taaatgcaaa
     8101 ttatgattcg ctttcagcta gttatgtatc tgattttggc agccctataa aaattgatgg
     8161 atccattttt agtgaccctg gaaaatatcg agtggtttca gagataacag gaatagactt
     8221 tgacaatacc tttttacctg aacctttaaa gtatgaatat aatatcaatg taaggtaaac
     8281 agaatctatc gtaattttat agtccaattt taaagggtgc aatcaaatat tagcaatcat
     8341 agtataatgc tagaactttt ttaaatgtgt gatatttaat aagatgaatg aaattcaaag
     8401 atcaaataat cgcaataaca ggtgcttctt ctgggatagg aaaacaagct gcaattgatt
     8461 tttcatataa tggagcaaag acaattattt taatttcaag aaatatcatg agattgaatc
     8521 aagtaaaaaa gaaattaaac ccagattgta ctgtagagat ctatccatgc gatatttcca
     8581 acaaaaaaat ggtttatgaa gtcggtcaaa atattatgaa aaaaatcgga gctattgata
     8641 ttttagtcaa taatgcagga ataggaattt atggaaaggt gattgaccaa acaatagaag
     8701 acattgaaaa agttacatca actaattatt tgggaatggt ttactttacc aaatcatttt
     8761 tacctgccat gattaaaaat aagcgaggtc atatagtaaa tgttgcttct ttagcagcta
     8821 gttttggaat tccatgtatg gcggcgtatt gcggatctaa atttgctatg ttaggatttt
     8881 ctgaatcttt gtcatatgaa ttaaaaaata caaatgtagg ggttaccgtg gtaagtccaa
     8941 ttgctgttaa aactaacttc tttgataatg aatcattcaa gggtaagatg cctcataaat
     9001 taggatatat acttgatccc aaaactgttt ctaaagctat acttaaagct gcatgttcta
     9061 aaagaattga aatcgttgtg ccattttttg tgagatctgc tgtatggtta aaacatactt
     9121 ttccatattt gatcaatcca ataataagtt cttcatttaa agatcaaaat tataattaag
     9181 ataatttagc aattaaggac tattaaatat aagatatttt tattataggc ttatacatat
     9241 tttattaact aaaacaatcc aaatacacta tctgagatga agaataaaag ttaaacataa
     9301 acatcccaaa tattttattt tattcgtcct tctcttctaa gatccgatat tattagcata
     9361 agtgtatcat aatcaatatt aacttcattt aaaataacac tatgatcagt ttttttattt
     9421 ttctttataa atttttcaac tagaattttt tttttttgat agtttctatc gatcatcgta
     9481 agaataaatg gatggatata tcttataaat ctaaaaaata agactagata agcaatttat
     9541 tatgttttgt atattttttc aatgtgaaga agtatagatg ccctaattgt acctctttaa
     9601 attccttaag agtaaacaga atttttgatg gcaggttatt atttacctgc gaaaaatgcc
     9661 atatatgttc agttttaccg ttttataata acttagacga aacctactta gagtttttag
     9721 acaggtttga taatggttat gtaactattt ttgaagacct gaaagtaata cttgaaaagg
     9781 aaaaattagt gcgttctaaa acagaaattg aaaaaatgat taaaaaaagt aaaatcaata
     9841 acaaccttat caatgaaata ttatattcag atttagatta tgttgttgac gttataaaac
     9901 tggaggaatc taaaatagcc ctcggaaaag aattagatga attaccgtta gacgatacta
     9961 tattaaacca gttaaagtca agaaatatct caacattata taaatttcaa gaagaagcta
    10021 ttttcaatat tcttcaagag aaaaatacgg taattattgc ccctaccgcc tctggaaaaa
    10081 cagaagcctt cagtcttcca attttacaaa aaatatatga cctaaagaac caactaaata
    10141 taaaaaatgt taccttctct tataatcaga atgagttcgg tactaaacaa ataaaagcag
    10201 tttttatata tcctacaaaa gcattagcaa gagaccaact atcgaaaata atggagtatt
    10261 caaaaaatat gaacattatg gtaaaaatat ttgacggaga cttaaatgca aatgaaaagc
    10321 aaaatttcta tgaatttcct cctgatatag taataacaaa ttttgattca ttacattatc
    10381 atcttttgaa taatacaaag cttataaaat tgttgaatac tttggaatat ttagttgtag
    10441 acgaagttca cacatatact gggatatttg gatctaatgt ccattacatt atcaaaagat
    10501 tagaaagaat cataaataat aaaaaattgc ttttaattgc atgttctgct acattacgaa
    10561 atgttaaaga attttgtgat ttattatttt ctcgtaaaat ggaaatagtg aatggcatag
    10621 gaaagaaagg gcacacaaat tttgctattt tatacccctc tcttagatca aataaatcat
    10681 tagttattga actaataaaa aaaattacca agaatcacaa aactctcgtt tttagtaatt
    10741 ctcatttatc tgcagaatta atagcattta cttcatcaaa acaaggaata aaaattgggg
    10801 tccacagagc tggtctccca cccaagaata gaaaggttgt cgaaaattct tttaagaatg
    10861 gaataataga tgttatttct tctaccccga ctttagagtt aggaatagat attggagatg
    10921 ttgatgcggt aatatctaat cttgttccta ttaatcgact cgtacaaaga ctaggaagag
    10981 cagcaaggaa gggacaagaa ggatatgctt tcttgacact gggaaatgat ccgataagtc
    11041 aatactataa aaatcatcct gctgactatt ttgttgacga agaaatacca tacatagatc
    11101 cttttaatcc aataataatg gaaaaccaaa ttctggctat gtgttttgat agacctattt
    11161 caaaacttga atcaaagaat tacttactaa cccttagcaa attggaaaga aagggattag
    11221 taatatttag taatggaaaa tatctcccta atcgagaaaa acaaaatata ctaaaaaatt
    11281 acagcataag aggaattgga aatgttgtag atattctttt taatgggaaa attattggaa
    11341 atagaaactt gccccaagcg ttggaagaat tacatgacga tgcaatttat tttctttctg
    11401 gaagaagata taaagttaaa aaattaatac ttgacgaaga gagtgaaaga aatttcgctg
    11461 atatagataa aataccaatg aactatccat actatacaaa atccataatg gaagagtatc
    11521 cagaagtaat agaaattttt gaagacaaag aaatttttgg aataaagtta aggtattgtt
    11581 ctttaaatat attcaaaaaa gtattaggat actcaaatat tgaaattgga aaggagatta
    11641 atcaaggaaa aaaagttttt tttagtgaac ctataaccta caattatact acaaaaggat
    11701 ttatatttag agtgccaagc ccgatagaaa ttttaaaaat gacttcagag aaagagaaag
    11761 tagaagtaag tggataccat gcaacagaac atgtgctaat tgaaggaagt agtatgttaa
    11821 caggaggtgc ttcacaggac attggaggaa tttcattagg ggatacagga atgatagtaa
    11881 ttcatgatgg cagtataggt ggtaatggag caagtaaaag tctttttgat aaatttgagt
    11941 tagccgttaa aagggctcat tcgatcctta tagaatgtac atgtgaaaat gatgatggat
    12001 gccctcgttg tacttattct tatcggtgtg gtaataacaa tgaatattta cacaagaaag
    12061 ctgcaattga agtacttact cgtattctta gtatggaaaa gacagattta ttagatttta
    12121 accgttctca acagactttg atatagaata ataaaggaat tctcgcgagt tatacatatc
    12181 atggtatcta ttttaatctt ttaataataa attattaaga attaaatttt agattattat
    12241 ctaacgtatt tttagatgat tcagagatta taccttgaag agagtctttt gtactatcag
    12301 ttatattttt tataacaggt gtaatgccaa ttttatctac gccctttaac acaccagtaa
    12361 aaaaagtttc ccatcctaaa ccaataacag ctagaaaaag tactatggca ataaaccata
    12421 aaattactcc catactatta tttagtacat tttttaagta tattaatagt tagtcagatt
    12481 tgttaagttt aaaattacat tatctatctt atagtaaata atacactatt ttaattagta
    12541 ataaataata taacacccta tataacgaag atgaatctgc taaaaatttc agcgataacc
    12601 ttaaaagtta aaagtatgag aaatgaatgg caattctatt caaaaattcc aggattaagt
    12661 ctatgctatg gaggacctga tagctcattt acatcctttg aaatgaccga ggagttcagc
    12721 aattcaaaaa catacatcaa tctagagttt acaaagggat taaaatgtga cactaatttt
    12781 ggcagaatta ttatttattc aaaaaatgtg gataatcttt atgattactt caaacaagat
    12841 caagatatat gtcgtttaat taccattaaa aataaaccag tagatggact atggggagag
    12901 aggtattttc acatattaga tcctgaaaaa tacgaattat catttgctca agttataagt
    12961 taaaattaaa aaattatatt cattttggtg gattcaaaaa aaaggaattt tactagatat
    13021 ataccaatat tatagtaact tatattacct tgataagaat agcttttgaa attgatatta
    13081 tatcatcttc ggcacctaac atcaaaatat aatttcccat agcaacatca ttagagagag
    13141 taaatacaaa agatatttct tttgattcat cctttttcaa ggctattgag ttttcgctaa
    13201 aaattccata agaagctccc aagtcaccac ttggcgttaa tgtagatgat gagataggat
    13261 gaagagaaga attattattt gcgttaatct cgaatctaat ttccttgctt tctcctggag
    13321 taagtgtcat ttcattgttc tcagttttaa ttgaaaaagg taatgggata ctggtattaa
    13381 cataaccaat cttgttttca ctccactcag tgaaccaaat tgatccattg taagtttcat
    13441 ctgtaagttg caatacattt ccaataccac aactattttc tggtaagtcc ggtttacatt
    13501 gtccaaatag tttgttctgt gacggaatcc aatattcaac tagtttgagt tcatctggaa
    13561 agaattttcc aattttgttt ccagtatgtt cattaaatat aatagagtta ttttgtcctt
    13621 ttttcatcca gtagggtaaa gtatatgctt gaccattaac ttcttctcca gcaaaaattt
    13681 ttttagatgc tgtagaggtt ataaactttg ttaaggaact gttaatagga tttaatttaa
    13741 caaatatgct agttccatgg tcacttaccc aaatattacc tgtctcatcg atagttatac
    13801 caacaggaga aggaaggtct cctagatcat attttttaaa tgatttattt gtaatatcat
    13861 acttataaat ttggccttta tatccaaaag caaggactga cacccatacg ttattatttt
    13921 ttttatctac tacaattgaa ccagtactga taacagaggg atcgaaatct ttaaaatctt
    13981 caacaggaag tggaatttta gcaattccat cggaggtact acttttcatt aaactcaaat
    14041 ttcccatcca taatgattgt gatctaattc ctgcaaagaa aatatgccca ttgttatcaa
    14101 aatcaattga aacaggatag acagtaccaa aagataagtt agtttcgggg attttaaaat
    14161 gttcaaactc cttattttgt ttactaaatt tccaaattaa attttgtttt tcatcagtaa
    14221 accatagatt ttcgttcgac ggatcaagtt caagatccca aacctgagag gagtcaatag
    14281 gattatcgcg cgacggccag gtcggtatat catattttgt aaaaatgctg gtttcaggtt
    14341 gatataaaat cagggacccc tgttttgtag aaatatacca taatccttca acattgtcat
    14401 cgtatgttat tgccaatggc atttcgcatt cattattcaa ttttatttct ttgatatatt
    14461 gattggaatt aggcttagaa ttaatcccac aaaattgatt tttaaatttt tctatagcct
    14521 gttgtttaga aaattcttgc tgtttggaaa actcttgttg atcgccaaag gcctgagtta
    14581 atatatcaga atacataaat tcatttatca aaaaagttgg ggaaaagtta gaatttagat
    14641 ttgatgaaga aataaaaata gttaaggtta gaaatgaaga gattaggaag gcataatatt
    14701 cattattttt actagagata ttcatctaac gatattagat tattagtcct ttatatctca
    14761 ttatatctca ttaagaactc cacacgtaat aatacacata ttaaaataaa aaaatattaa
    14821 aatttactgg aaatcaatct atttagtgtt tgttcaaaaa tttcataagg taatgcacca
    14881 ggaattatgg actggatatt attgtctttg atcagtataa atgaaggagt tccagataat
    14941 cccatcgatc tagcaatatt atcattattt tgaacaatga gagaatactt attggattct
    15001 atgcaatcag aaaattgttt tatattcggt atctggatgt tttttgcaaa ttgttttaga
    15061 ttatcacttg ttatccatcc agttccttct cctttccaat tatcgtacaa ttcagtatga
    15121 taatcccaat acttgccttg ctctccggca caatatgatg cttccgctcc catagtcgac
    15181 cctttatctg ttggaatatc atttacaata tagtctttaa atataaaatt aattttaccc
    15241 gaatctacaa agtttttaat aatttgttcg cgtgtctcat tatggaattt tgaacaaaat
    15301 gtgcattgat aatctccaaa ctcaaccatt gtaatattag aagagtttcc caatatcgga
    15361 acaaagtcag caacaggctt taataagtaa gaagaaaatt gatttaataa cccttgctca
    15421 ttataataat tgaaagatgt aagcattttc tcataaattg gcagattacg ataataatcc
    15481 gtataatttg aggttaatga aattatatac agatttgctc cctggattat tccaacatca
    15541 gcgttttgaa tcttattttt atttccatct atatatgaat aaataagcct gtgattatta
    15601 tctttgatat tcgaaagttg gctcgattca attaattcaa aatcatcaag tgtcctaaca
    15661 aattcatcta gtgtagaatc taccacagat tttattgaca gagaatcact tgaaacagga
    15721 gatacattaa tttttatttt atcaattgaa tcacgatcgg gagcaaatat tacagaatca
    15781 gtagtttcgg taaatatcca gttcttcgga taatctattt ttagaaagtt gttttcataa
    15841 gtagtaaaat ttggagttac ttcagtattt gagggtattt tccattctgc gctaacatag
    15901 tgtagataaa cattcaagag aactgcactt ataaagatgg ttgatacaag tttatatttt
    15961 aacaatatta ttcttattaa aaatatcata ctatatatta aattataaaa taatttttgt
    16021 taagtttttt gtcattaaat ctttattaaa gaatttatac attaaataca tcaatgaaga
    16081 aaatacatat acttaatatt attccaacaa atcttaattg ataaaataga taatagattt
    16141 agaaagaatc aaaccaaaat aaaagaagaa tcacaattaa taatatgtta ttcaaaatgg
    16201 tcaaaatcct tcctatccca ttgtatacat ctcacaacaa tttcgcaaat ttattctata
    16261 aagaaggtta tttaaatata aaactttcct gttttcaaga cgaatatctt gtaagtccgt
    16321 catatacaaa acataagata acaaatgttt ttccc
//
LOCUS       DASVEV010000266         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1501843, whole genome shotgun sequence.
ACCESSION   DASVEV010000266 DASVEV010000000
VERSION     DASVEV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501843"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF222_11650"
     CDS             complement(<1..186)
                     /locus_tag="VF222_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143120.1"
                     /translation="MILLSQYQENKNERKKEMNVLTQQIVLETIQELNSMENEGVTYE
                     QIKRRLREKYPDLPLSNL"
     gene            complement(683..1126)
                     /locus_tag="VF222_11655"
     CDS             complement(683..1126)
                     /locus_tag="VF222_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143121.1"
                     /translation="MHLNLLHNNEKCSESKEREKKLITKSKHKNYTLRYFYNIDNIFF
                     HLFLHTIVYDKRTNEKIEQQGILLRSWNPLTKRGERYVFKKGEEGEGDLNLKIVYLPN
                     TKTIFEFSTIDDKNQGSYNLAEEIINLSPHKDIELKFINTPSILS"
     gene            1678..2517
                     /locus_tag="VF222_11660"
     CDS             1678..2517
                     /locus_tag="VF222_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7143122.1"
                     /translation="MSEFEPITYKLKLISNWNTVANKYHNNWVQNDIGPFGATEKLIH
                     IADIQKSDFILDIGCGTGAVTKKISKKLCKKGKLIGIDISRGALSIAKSEIKYSNVDF
                     IEMDAEKIYFSLKFSKVISQFAAMFFTNPIESLYSIRKLMAQEGMIVLGVHGPAENVP
                     YFSCIMNNIMKFIPNLIPSGSPSVHSFAERNKLKDLLIRTGFCNVKIEKYQFSYNAGT
                     FEQYWHNYMACTANSIKEIIEKDNDLLGLIKYESEKNALLFMENNNITFPWEILIASA
                     FNP"
     gene            complement(2572..3171)
                     /gene="tmk"
                     /locus_tag="VF222_11665"
     CDS             complement(2572..3171)
                     /gene="tmk"
                     /locus_tag="VF222_11665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7143123.1"
                     /translation="MSGGQIIVLEGIDKAGKGTQCKLLHNAITRAGMDCIILDFPDYT
                     TPIGKEIRLILDGRRSYSNEVQHLLLSVNRWEKKEEIEKILQRGTTIIMDRYYQSNLV
                     YGLSHYLDLNWLINLDRGLPKEDLVIILEIDPETSYRRVSHNRDIFEKNLEFLVTVKQ
                     NYRKLSEVYGWKIINGEEKVEKIHDKICGLVKEKLDILI"
     gene            3353..3796
                     /locus_tag="VF222_11670"
     CDS             3353..3796
                     /locus_tag="VF222_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143124.1"
                     /translation="MQFWKKKQEFCFLCKKKLEHKYKPHKEWDIKGMLCSDCHIIKTK
                     EYIVKKQLEKEKLEKELDTCSICGLHIKSESEKVKPRWQWNLERGTVLCNNCYEKKEN
                     KFEKERNFCFICGTHLKFFRYNPKPNWKLQGQLCRNCWDNRNEIQ"
     gene            3847..3921
                     /locus_tag="VF222_11675"
     tRNA            3847..3921
                     /locus_tag="VF222_11675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3882..3884,aa:Arg,seq:gcg)
BASE COUNT         1394 a          556 c          551 g         1449 t
ORIGIN      
        1 taaattagat aaaggtaagt ctggatactt ttctcttagt cttcttttta tttgttcata
       61 tgttactccc tcgttttcca tagaatttaa ttcctgaatt gtttctaata ctatttgttg
      121 tgttaataca ttcatttctt tcttcctctc gtttttattt tcttgatact gtgatagtaa
      181 aatcatcatc gtggatacct gtaactatgt gtaatctcat atcagacttg tttagcattc
      241 aaatatttct tccaaaatat tatctatcac atttctaata acgctttacc cttccttttt
      301 ttaacgtcct gtatgtcata aatctataat atttatgaat tttcttctct tttctctacc
      361 agtttcatct taaacgattg tatacgttgt tattttgtaa gaactagatt tatctttctt
      421 aggtgaaatc tagaacaaaa tattcacgaa ctaaaaacat ggtaagttaa attgttctat
      481 aaataaagac aacaacattc aggtaactaa aatttttgat tacaaattca gtaattgcct
      541 tacgttttta aaaaacggaa gaaactaata aaaatgattt agtaaaattt gttatttttg
      601 aagatttata taaacaaatt agaaattaga aggaaagatt aatgaatatg aaattcactt
      661 ttgataaaca aaagacaacc atttacgata gtattgatgg agtattaata aattttaatt
      721 ctatatcttt gtgggggctt aaattaatta tttcttctgc caaattataa cttccttgat
      781 ttttatcgtc tatagtggaa aattcaaata ttgtttttgt atttggcaaa tatacgattt
      841 ttaaattcag gtctccttct ccttcctctc cttttttaaa gacgtaacgt tctcctcgtt
      901 tagttaaagg attccaactt cttaataata taccttgttg ttctatcttc tcatttgtcc
      961 gtttatcata aacaattgta tgtaagaaaa gatgaaagaa aatattatca atattgtaaa
     1021 aatatcttaa agtataattt ttatgctttg attttgttat taattttttt tctctttctt
     1081 ttgattcaga acatttttca ttattgtgta aaaggtttaa atgcatacta ttgtagtaat
     1141 ttgttagtta tattatttag actcaattta atttgaggtt tttaatttta ttttttgagc
     1201 atcatatatt gttgttcaat gagttttata gttattagca aatagattat cttcttttta
     1261 tctttatata ttcctgagat tctttgatga ctaacgataa atacattaca aagtttttcc
     1321 atgaaatatt gaaatataaa aatcaataag atcttgagtt ttttatatcc tattgctatt
     1381 ctcctgaaat taaagaaaat tatcacaaga ttatttaaat ttgtattcca agaatccaac
     1441 gtgagaatta tatgagaata attctctgtt gttaggttgt aaaatgccgt gtctttcaaa
     1501 tccattattc tggatctaag tccatgtgtc aaaggtcttt gttacaaggt taatgtaaaa
     1561 aattgtttta atatatacaa ataaaaaaca atagaagtct gtaaaatgct tcattagcat
     1621 gcagagaatc actctcttaa atatatataa taaacgagca aatcaaaaaa taacaaaatg
     1681 tcagaatttg aaccaataac ttacaaatta aaactcatat ctaattggaa tacggtagca
     1741 aataaatacc ataacaattg ggttcagaat gacattggac cttttggtgc tacagaaaaa
     1801 ttaattcata ttgcagatat acaaaaatct gattttattt tagatatcgg atgtggaaca
     1861 ggagcagtaa caaaaaaaat atcaaagaaa ttatgcaaaa aaggaaaatt aattggaata
     1921 gatatttcaa gaggagcact ttccattgct aaatctgaaa taaaatactc caatgtggat
     1981 tttatagaga tggatgctga aaaaatttat ttttcactaa aattttccaa agtaatcagt
     2041 caatttgcag ctatgttttt tactaatcct atagaatcat tatattctat aagaaaatta
     2101 atggctcaag aaggtatgat agttttgggt gttcatggac cagcagaaaa cgtcccatac
     2161 tttagctgta taatgaataa tattatgaaa tttattccaa atctaatccc aagtggatct
     2221 ccttcggttc attcatttgc agagagaaac aaattgaaag atcttttaat aagaaccgga
     2281 ttttgtaatg tcaaaataga aaaatatcaa ttttcatata atgctggaac atttgaacag
     2341 tattggcata attacatggc atgtacagcg aattctataa aagaaataat tgagaaagat
     2401 aatgaccttc ttggcctaat taaatatgag tcagagaaaa atgctttact gttcatggaa
     2461 aataataaca ttacatttcc gtgggagata ttgattgcat ctgcatttaa cccataatta
     2521 cattaatgat taacctattg atttttatcc aacacaaaag ttaggttatt attaaattag
     2581 aatatctaat ttttctttta ctaggccaca aattttatca tggatttttt caaccttttc
     2641 ttcgccattg attattttcc atccataaac ttcagacaat tttctatagt tttgtttgac
     2701 agttactaaa aattcaaggt ttttttcaaa aatgtctcta ttatgactta ctcttctgta
     2761 tgacgtttca ggatctatct caagtataat aacaagatct tcttttggta gtcctctgtc
     2821 taagttaatt agccagttca aatctaaata atgagacaat ccatatacaa gattagattg
     2881 ataataccga tccattataa tagtagttcc ccgttggaga atcttttcta tttcttcttt
     2941 tttttcccat cgattaactg atagaagcaa atgttgaact tcgtttgagt aagatcttct
     3001 tccatcaaga atcaatcgta tttcttttcc tataggagtc gtgtaatcag gaaaatccaa
     3061 aattatacaa tccattcctg ctctagtaat cgcattatgc aatagtttac attgagttcc
     3121 ttttcctgct ttgtctattc cttctaatac tattatttga ccgccgctca ttaaaaatat
     3181 aaatcaattt tgaaaatact ctaattatat ttattgaaat ttcgctaaat tatcaattaa
     3241 attacaagac taagatttaa aaaataaaca atcataaatt ggctttatta atattaattt
     3301 ataacactta ttatattata atgagattat cagctaaagg tgagatgaca ttttgcaatt
     3361 ttggaagaaa aaacaagaat tttgtttcct ctgcaagaaa aaacttgaac ataagtacaa
     3421 acctcataaa gaatgggata taaaagggat gttatgcagt gattgtcata tcattaagac
     3481 aaaagaatat atcgtgaaga agcaactaga aaaggaaaaa ctggaaaaag agctggatac
     3541 gtgttccata tgtggcctcc acattaaatc tgagtcagaa aaggtaaaac caagatggca
     3601 atggaattta gagcgaggaa cggtattgtg taataattgc tatgaaaaaa aagagaataa
     3661 gtttgaaaaa gaaagaaatt tctgctttat ctgcggtact cacttgaaat tctttaggta
     3721 taacccaaaa ccaaattgga agttacaggg ccagctttgt agaaactgct gggataacag
     3781 aaatgaaata caataacaaa taatactgta aaatttataa ttcacaatag cgagatagat
     3841 atgtgtgggc tcatagttca gtttggatag aatgactgcc tgcggagcag ttggtcgtgg
     3901 gttcaaatcc ctctgagccc gatttgataa cactgctttt attttggccc
//
LOCUS       DASVEV010000267         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_726874, whole genome shotgun sequence.
ACCESSION   DASVEV010000267 DASVEV010000000
VERSION     DASVEV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726874"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF222_11680"
     CDS             complement(<1..252)
                     /locus_tag="VF222_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143125.1"
                     /translation="MKNHFSKNMVIATMAIVGTLLLVSTIINNSSSNMSVNAQAQQQQ
                     SSKEAPEKGTIFVFNEKSRPANEPEKCAALASVVKGKALP"
     gene            816..1343
                     /locus_tag="VF222_11685"
     CDS             816..1343
                     /locus_tag="VF222_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143126.1"
                     /translation="MKKSKDNVVDNYNDDDETRVYSSYHPSQFDNALLNEAIQKGIKD
                     KEFINSSLSLLKNAKFPIYKFKIIEHVQQLTNDKITIALFQTLSDSLEYKNIEQIRNI
                     FQGNIPAKNSPSRTKSPEGLNVNPLTTKQTQKSPTSNNNDSVSDDTMREYTCNKCGKP
                     FLTREDLHIHQKFEG"
BASE COUNT          804 a          273 c          296 g          746 t
ORIGIN      
        1 aggcagtgct tttcctttaa ctacactggc caaggcagca catttttctg gctcgtttgc
       61 tggtctactc ttttcgttga aaacaaagat agtgcctttt tctggtgctt cttttgatga
      121 ttgttgttgt tgagcttgag cgtttacaga catattacta ctactattgt taattattgt
      181 tgaaactaat agtaatgttc ctacaattgc catcgttgct attaccatat tctttgaaaa
      241 gtgatttttc atttgcgtaa gcattgtaac aaaattatat ataagaattg tagataattt
      301 tacctagata tccaattata atttataatt atgctctaag atacaaacaa aggctaaaaa
      361 agattgtatc aaccattaaa gtgaaataac taagagatat gaagaacaac aacaatagaa
      421 tgatgtttat cattttttag tggaatattt tactgcataa ttaattaatt aattaattgt
      481 catagccgaa tgatgaaatc cgtctaagat aatatgatta tgttccgact atgacaaatc
      541 aagtgatgat tgattgtttg agagatgaga tatttgatag cctattatag tgaatattgt
      601 atttagtatt gtttgtaaac ctatgttgat aaataatcaa caatctattg gtactatata
      661 ttaaaagatt gattagttga cttattcgaa aataaatcag ataaggttgt ctactgttta
      721 aagatgttaa caaatatatc aacataaact tacctaagga caaagctaat cgatatgttg
      781 aaactattat taaaattgtt ttacagttgt acatgttgaa aaaatcaaaa gataacgttg
      841 ttgataatta taatgatgat gatgagacaa gagtatattc cagttaccat ccttctcaat
      901 ttgataacgc attattaaat gaggcaatac aaaaaggcat caaagacaaa gagtttatta
      961 attctagttt aagtttatta aaaaatgcca aatttcccat ctataaattc aaaataatcg
     1021 agcatgttca acagttgaca aatgataaaa ttactattgc attatttcaa acattatccg
     1081 attctttaga atataaaaac attgaacaaa taagaaatat ttttcagggt aatattccag
     1141 caaaaaattc accttcaaga acaaagagcc cagaggggct taatgttaat cctcttacta
     1201 caaaacaaac tcaaaaatca ccaacatcaa ataacaatga ttcagtttca gatgatacta
     1261 tgagagaata cacttgtaat aaatgtggta aaccttttct tacgagggaa gatttacata
     1321 tacatcaaaa gtttgaagga taatatgttt tatgttaaaa tcctaatagt aaatgtcatt
     1381 aatagagttc actttcgaat agtagagttc atctgcatat taaggaagct ggtctggagt
     1441 agagccttcc atggagtagg gttctattac atagtattta gcatttttat tattagtaat
     1501 atattaaaaa atatataaaa atcaaaattt ctttaataat ttgtcattta tatattatat
     1561 ttgggatttt ctgttatgaa tagcatcttt ttggattaat atgaacacca tttgataaaa
     1621 agaaattcct aatttgaatg cctcaaaatc tatatttctt aaaatgtatt ttatggattc
     1681 attatctgat gaagacagta taaattcatc tataattttt ttgaatttat aatcattatg
     1741 gtgttgaaac atgacttgat tttatcaata tagttattaa gagcttttag aaatgttggt
     1801 gactaatgcc taacatttga aataataaca ttctagaaaa accaataata aggtattcaa
     1861 caagctgctc aaacagtaaa acaaaagtta gaatctaaat ctttttagat tcttaatttt
     1921 acgtttttaa aaaataagat ttaaaatgtt tattagcaaa agaatcttat gaaacaagca
     1981 tattttatta tcgcattaat tgcagctctt acaataggcg tactatctat aaattcagga
     2041 atcacatttg ctcaagaaaa aaatgaaact gcagaaatca ataataaccc tttaattaca
     2101 acatcacaaa tagatgaat
//
LOCUS       DASVEV010000268         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_920222, whole genome shotgun sequence.
ACCESSION   DASVEV010000268 DASVEV010000000
VERSION     DASVEV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920222"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1379
                     /gene="pstC"
                     /locus_tag="VF222_11690"
     CDS             396..1379
                     /gene="pstC"
                     /locus_tag="VF222_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7143127.1"
                     /translation="MTKYNDNDSLIPLTKLSKFDPIFKLILVGAAAYTLIMLALILFA
                     VGEGSKEIFQDEGLNFLTNTDWNAVPGRESFGALPYIIGTLVCSGIAMAIGVPISLGI
                     AIFLSEIAGTKLKTSLSFIIELLAAVPSIIYGFWALFVFRFWIMDFVEKPLHNFFGDS
                     LPFFAKTPFGIDVFTAGIVLAIMIIPIISSISRELLLVVPNSQREAAYSLGATRWEAI
                     KIAVLPYAKSGIVGASIIGLGRAIGETMLVTLIIGNATGLNAIPTSLFSQSQTLSSLI
                     ANEFNEAASELHLSALIGLGAVLLLLTMMINIIARLLVYKMVKTSNPGGRI"
     gene            1383..2309
                     /gene="pstA"
                     /locus_tag="VF222_11695"
     CDS             1383..2309
                     /gene="pstA"
                     /locus_tag="VF222_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7143128.1"
                     /translation="MSSKTKENNQYIKRRKNVQEMMSNTSNRKIYVNKLVTILCVCCI
                     IIAIIPLGSILFEVFKNGSSAISFEFLTQKPGSIGSGKGGIGPAIQGTLIVVSSASLI
                     AVPVGVFAGIYLSEYAKSNNKFAFAVRFFADVLTGLPSIIVGIVSYVIIVISIGSFSV
                     IAGAFSLSLIMIPLVTRITEESLKMVPNSIREAAYSLGVPKWKITLFIVLKTATSGVL
                     TGIVLSIARIAGETAPLIMTILGTSLYFSSFTGPVDALPLRIWRLASLPYESAHASGW
                     GAAMILILLILGLNVGLRVLARKQNVATSKDT"
     gene            2429..>2664
                     /locus_tag="VF222_11700"
     CDS             2429..>2664
                     /locus_tag="VF222_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7143129.1"
                     /translation="MTTYMDGSEVRKIQFTGKSTFVLSLPKKWVNELNLKAGDPVTII
                     REPNNTLSIISDITRSSIEAVDEATVEVLRDEGG"
BASE COUNT          868 a          383 c          455 g          958 t
ORIGIN      
        1 gacttaaatc tccacatata ctatttttta attttttatt agatttttaa attaaacgga
       61 tcacagaaag tttagttttt atttgttata attatttcag aaaactttgt atttatttgt
      121 aatttttaca atatttagaa atggcacttt gttttgttat aaccagttta attattataa
      181 aaagaaggat gattttgaaa ccaaacttta ttttaaacct agtccatttc aacactagga
      241 aaaagataga gaatacaaaa ttgtcaatat tctatgacta gaacttgtac taatcaatat
      301 ctcaatagac tatatggatc tatattaaaa ctatatgata cataaaaaaa tatcaaaata
      361 tcatagttat tagaggggtt acattcacca cgaatttgac aaagtataat gacaatgatt
      421 ctcttattcc attaacaaaa ttatcgaaat ttgaccctat cttcaaatta atattagttg
      481 gagcagcagc ctatactctc atcatgttag ccttaatact ttttgctgtt ggagaggggt
      541 caaaggaaat ctttcaagat gagggattaa actttttaac taatactgat tggaatgcag
      601 tacctggtag agaatcattt ggcgctttgc catatataat aggaactttg gtttgctcag
      661 gtattgcaat ggcaattggc gttcctatta gtttaggtat agctatattt ctatcggaaa
      721 tagcaggtac aaaattaaaa acttctttat cattcattat tgaactttta gccgcagttc
      781 caagtataat atatggtttc tgggcattgt ttgtttttag attttggatc atggattttg
      841 tagaaaagcc tttacataat ttttttggtg attctttacc attttttgct aaaacacctt
      901 ttggtataga tgtatttact gctggtatag ttcttgctat aatgataatt ccaataattt
      961 cttctattag tagagaacta ttacttgttg tacctaactc tcaaagagaa gctgcatata
     1021 gtttgggtgc aactagatgg gaggctatta aaattgctgt attaccttat gctaaatctg
     1081 ggatcgtagg tgctagtata atagggcttg gaagagcaat aggtgaaaca atgttagtta
     1141 ctttaataat aggtaatgca actggattga atgctattcc aacttctctt ttctcacaaa
     1201 gtcaaacttt gtctagttta atagccaatg aatttaatga agctgcatca gaattacatc
     1261 tttctgcact tattggtctt ggagctgttc tacttttgct tacaatgatg ataaatataa
     1321 tagcccgact tttagtctac aagatggtta aaacttctaa tcctggagga cgaatataat
     1381 acatgtcatc taaaaccaag gaaaataatc aatatatcaa aagacgaaag aacgttcagg
     1441 aaatgatgtc aaacacatct aatagaaaaa tatatgtaaa taagctagta acaattttat
     1501 gtgtatgttg tattattatt gctataattc ctcttggtag tattcttttc gaggttttta
     1561 aaaatggttc ttctgctatt agttttgaat ttcttacaca aaaaccaggt tcaattgggt
     1621 ctggtaaagg aggtataggt ccagctatcc aaggaacttt aatcgtagta agttcagcat
     1681 ctcttattgc tgtacctgtt ggtgtttttg ctggaattta tctatcggaa tatgcaaaaa
     1741 gcaataataa gtttgccttt gctgtacgct tttttgctga tgttttaaca ggactacctt
     1801 caattatagt tggtattgta agttatgtga taatagttat ttctattggt tctttttctg
     1861 ttatagcagg agcattttca ttatctttaa taatgattcc acttgttact cgaataactg
     1921 aagaaagcct taaaatggtg ccaaattcta ttagagaggc ggcttactca ctgggagttc
     1981 ctaaatggaa aatcacttta tttatagtac taaagacagc aacgagtgga gttctaactg
     2041 gaatagtatt atcgatagca agaattgctg gagaaactgc tccattaatt atgactatcc
     2101 taggaacaag tttatatttt tcatcattta ctggacccgt agatgcgctt ccactgagaa
     2161 tatggaggtt ggcgtctcta ccctatgaat ctgctcatgc tagtggatgg ggtgctgcta
     2221 tgattttgat tcttcttatt ttaggactaa atgttggatt aagagtgctt gctcgtaaac
     2281 agaatgtggc cacttctaaa gatacctaat tgaattaaat ttttgggatc tcaactatct
     2341 ttagttctat atataacata tagaaatcta tatacctata ttctaaataa tcgtaaacta
     2401 tacatgtatt attactgttc ataaactatt gactacatat atggatggta gtgaagtaag
     2461 gaaaatacag tttacaggta aatcaacatt cgtattatcc ttacctaaaa aatgggtgaa
     2521 tgaattaaat ttaaaggctg gcgatcctgt gaccattatt cgcgaaccaa ataatacttt
     2581 atctataata tctgacatta caagatcctc aattgaagca gttgatgaag caacggtaga
     2641 agtattaaga gatgaaggtg gtaa
//
LOCUS       DASVEV010000269        30675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_143574, whole genome shotgun sequence.
ACCESSION   DASVEV010000269 DASVEV010000000
VERSION     DASVEV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 30675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30675
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143574"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="nadX"
                     /locus_tag="VF222_11705"
     CDS             complement(<1..813)
                     /gene="nadX"
                     /locus_tag="VF222_11705"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX7143130.1"
                     /translation="MKKKISIIGCGAIGSELALSVDSIMAKNVTLLSLLDIRPDNAET
                     LKSKLSNNNPVIFNNFSDFINSESFKEVELVVEAASQNALTSYLNQLILFKKDVLVMS
                     VGAFANSDFFSEVVGNVEGNDINIYVPSGAIAGIDALKSVKNSISYVTLTTTKNPNSL
                     KDSPFFKNTNLTVDSIKKRTLIFEGSAIEAVQNFPANVNVAALLGLAGIGVEKTKVNV
                     IADPSIRINKHEIKVIGKFGELIVRVKNIPSSTNPKTSYLAILSVIECLRSIT"
     gene            complement(997..1935)
                     /gene="nadA"
                     /locus_tag="VF222_11710"
     CDS             complement(997..1935)
                     /gene="nadA"
                     /locus_tag="VF222_11710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7143131.1"
                     /translation="MDQYIEEISSLKKERDAIILAHNYQLPEVQDVADFTGDSLALSR
                     KAEKTNAKNIIFCGVHFMAETASIICPDKDVFIPDINAGCSLASSINCTELLNWKKDH
                     PDAIVVSYVNTSAEIKALSDYCCTSSNAVKVVNSISPNNEILFLPDMFLGSYVSEVTK
                     RKNMFIWPGECHVHAGIRAEDINKMMKDYRNAEFLVHPECSCTSSTMYHMSKGDLLNE
                     GHILSTEGMMERAKNSSKTDFIVATETGILYRMQKENPTKNFIPIRKDAVCQYMKKIT
                     IDKVYNSLVNNVYQVRVPKSIADKAKSAIDRMLSIG"
     gene            2092..2274
                     /locus_tag="VF222_11715"
     CDS             2092..2274
                     /locus_tag="VF222_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S30e"
                     /protein_id="HEX7143132.1"
                     /translation="MPTHGSLTKAGKVKGQTPKVQGKTRVSPISKVRNKENFFKRFEK
                     RVPPGQKKPERGGRRR"
     gene            complement(2298..3167)
                     /gene="nadC"
                     /locus_tag="VF222_11720"
     CDS             complement(2298..3167)
                     /gene="nadC"
                     /locus_tag="VF222_11720"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7143133.1"
                     /translation="MKKLSDHFNLRKSLLNFLQEDVGTGDITSENLENSNKSVTAKII
                     FKSDFIGIVCGIEEVELLFDICNCNSTPHTLDGMKISPKENIMEIYGTAKDVLKAERI
                     ALNLLMRMSGIATSTRKFVEIVHKIDPSISIASTRKTVPGLRLFDKKAVVIGGGISHR
                     IRLDDMVMIKDNHIAVDKSIRKIIPSIRKKIGSSLKIECEVKNEREAIEAIEAGADLI
                     MLDNFSILEAKKTMLKIMDEGLRNLVKIEISGGINLANVLNYAKLKPNIISIGYLTHS
                     APALDFSLRVIDK"
     gene            complement(3167..3355)
                     /gene="spt4"
                     /locus_tag="VF222_11725"
     CDS             complement(3167..3355)
                     /gene="spt4"
                     /locus_tag="VF222_11725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HEX7143134.1"
                     /translation="MPRELACKKCKAITVGKVCPICNSTDLSGDWSGIVIVFDSKTSL
                     IAKTLEISKPHKYALKVT"
     gene            complement(3359..3949)
                     /locus_tag="VF222_11730"
     CDS             complement(3359..3949)
                     /locus_tag="VF222_11730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HEX7143135.1"
                     /translation="MFYIVSMTDIVRIPPSRLTDSLRNTAIGILKEKYESMISAELGY
                     VIMIIDANADSVGKLVTGDGATYHKVTFKALTFFPKIQEIVEGEIIEITDFGAFVRIG
                     PTDALLHLSQITDDYLKSDIKQGMIIANQSSRSLRIGSKMRARITAVSLGKGSSMGKI
                     GITCRQPFLGSFDWIKEEIEKAQTPQSPKEKKGVAK"
     gene            4110..5231
                     /locus_tag="VF222_11735"
     CDS             4110..5231
                     /locus_tag="VF222_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX7143136.1"
                     /translation="MVTVDQVLTSLKKVIDPELHKDIVSMGMIKEMAINNDKISFTLE
                     LTTPACPFNSEIEQDVRNAISNLGVKDLDLKVTAKVMEGRAISLDELLPGVKNIIAVA
                     SGKGGVGKTTVSVNLAIALAKTGAKIGLLDADIYGPSVPLMLGLKDTPQVVDGKIQPP
                     ISEGVKIISMGFFYEQSQQAGIYRGPIISGIIKQFLTDVNWGELDYLIIDLPPGTGDA
                     PLTIAQTIPISGILVVTTPQEVAMNVAVKAIGMFNKLNVPIIGVIENMSYLECPHCNS
                     DIHIFGTGAGQKISEQFKIPYIGDIPLHPQIMQGSDRGKSVIISDPESKQTKSFLKIS
                     KLVAGRVSVLAAEMQAQEQYEEKQENTEKNNLKQAENAS"
     gene            5221..5754
                     /locus_tag="VF222_11740"
     CDS             5221..5754
                     /locus_tag="VF222_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HEX7143137.1"
                     /translation="MPLKDEHVNILKKPFGELIKQEEISKEKLMSLVKDSKSLITVGD
                     ATTETIISFGLMPNLSIIDGVEKRIRRTESKILDLKNFFITSTSKFNQYECKNPKGTI
                     SKEAYLTIKKILLKGEQAIIFIDGEEDMLALPVFALAPINSVVFYGQPLEGLVSVKIN
                     DKIKNKSRALIDSIGLE"
     gene            complement(5861..5998)
                     /locus_tag="VF222_11745"
     CDS             complement(5861..5998)
                     /locus_tag="VF222_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143138.1"
                     /translation="MQNPRNVCSFCNSHIAEGQSICKDCIKLYGIKIGDHEKDGCGCD
                     T"
     gene            complement(6271..6534)
                     /locus_tag="VF222_11750"
     CDS             complement(6271..6534)
                     /locus_tag="VF222_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143139.1"
                     /translation="MNTKIEYSGKVYLYSSGMPLELIDLAKSKLVKEHSVLSEDIVLI
                     EVPEGVPEGAVMVTIWPHYLSVSKVKKVRDGSIYAPQLFNIDL"
     gene            complement(6630..6800)
                     /locus_tag="VF222_11755"
     CDS             complement(6630..6800)
                     /locus_tag="VF222_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143140.1"
                     /translation="MGSRKRRTIKPQRKTRSVEGRCPKCTTIVRFNVSGPVGDEIYEC
                     KGCMSKYHIDEL"
     gene            6951..7235
                     /locus_tag="VF222_11760"
     CDS             6951..7235
                     /locus_tag="VF222_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143141.1"
                     /translation="MTLPKKCSFSNQDTQCEIPPSYLLSVTENGEYLIGVSCMEHLEP
                     IKLKIKKLQINKTIPNGKLKVENIKMLSTNCIKGSQEDYEDVYSQRLREN"
     gene            complement(7262..8254)
                     /locus_tag="VF222_11765"
     CDS             complement(7262..8254)
                     /locus_tag="VF222_11765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7143142.1"
                     /translation="MERKKIFITRKINPIGIELLKNYYDVILTDTTESPTKNLMIQKV
                     KNVYGILCTLSERIDSDIMDAAGSNLKVISTFSTGYDHIDIREATKRGIYVTNTGNIL
                     SEATADLTFSLILALSRKIVEGHLYIINGKWKKGWTPDLFLGSNIYGSTLGILGLGNI
                     GAAVAKRAKGFNMKIIYNNRHQLSKSIEEKLNATFMNFDQLLQNSDFLSIHTSFEKDN
                     FHLIDTKELKKMKKKSFLINTSRGSIINQTSLIKALKNKWIAGAGLDVFEIEPLPKHN
                     KLIKLKNVVLLPHLGSATVQTRNQMSEVAAINLINILSNKKPMFLINKELEQKT"
     gene            8357..9541
                     /locus_tag="VF222_11770"
     CDS             8357..9541
                     /locus_tag="VF222_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX7143143.1"
                     /translation="MKRGTVNLPLHGGHAPSYLVKRMIKLSSAICTIIIEENGTKEFF
                     KRISDPLWFQAFGCVLGFDWHSSGLTTVVTGVLKQSLDPENHGISISGGKGRNSTKPK
                     YEIPQLAEKNHNLSTKEINNLLYASKMSAKIDNAAIQDGFTLYHHVIFFDEYGNWTII
                     QQGLNEKYKMARRYHWISDDLINYIIEPHKGIIGNERIPLCLNMTSKESEENRKNCVD
                     LIKEGSSSIKSSIYKIRETKTFENLDRWIHNSRLQHNNKMCLEPRLSNIEKYEMPRTI
                     SWKTIDDLYDITPTNYEDLISQKGVGPSTIRALSLISEIIYGNRASWKDPVKYNYAHG
                     GKDGLPYPVARNTYDKSIKYLSEVIGGSKIEKQEKISSLKRLSTYSNILFNTKEYTYT
                     VK"
     gene            9730..10050
                     /locus_tag="VF222_11775"
     CDS             9730..10050
                     /locus_tag="VF222_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143144.1"
                     /translation="MGSSIENYIYAQEGAITPEGSINDTMSGENATEWANATLAFAQE
                     GFTTEDLINETIDLGNSSTSNATLAFAQEGAITPEGSINDTMSGENATEWANATLAES
                     WDIK"
     gene            10625..11524
                     /locus_tag="VF222_11780"
     CDS             10625..11524
                     /locus_tag="VF222_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX7143145.1"
                     /translation="MEIFDNISPGNQLAKNIYGTCHPFIKWAGGKSRLISQIKNFIPK
                     EFNNYFEPFIGSGALFFYLVSSFKIKGNSSAHLSDINPELINVYKTIKDNLKDLIKEL
                     QYNEIEYYKDSKKFYYELRDENFGFNSIKKAARFITLNKTCYNGLYRVNKKGLFNVPI
                     GSYINPKICDQEKLNIVSKILNLTNTIIENYDYQKAIIKIKENDFIYLDPPYYPLNKT
                     SNFTNYTSYGFDYMEQKRLADFFNELDKKKCKIILSNSDTAFIRDLYSSFKENTISLN
                     TLRSINSNADKRKNHSELIIKNF"
     gene            11585..12094
                     /locus_tag="VF222_11785"
     CDS             11585..12094
                     /locus_tag="VF222_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143146.1"
                     /translation="MKDKKIFNGVKIIYISLIILTFIGFFNNAFAFFHDQGSHTNRVI
                     EESKKIIESLFNRVNIASNNYQNEIAILNLSMNNNVTKVNKTQEYIDNLKEIISSANK
                     TTVQQDYKPVLNNYIDSIQNELESYVHYNKYLITGNLTENQISINLLSKAFNNETQAI
                     NEYKKLELQ"
     gene            complement(12703..14697)
                     /locus_tag="VF222_11790"
     CDS             complement(12703..14697)
                     /locus_tag="VF222_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7143147.1"
                     /translation="MIFKALYFILLVFFSLSISGIVFVITTVYSHENTEFSQKNKTPI
                     DHVIVISQGRRSFDNYFGSYEGTKGFPKNMGIPINPFEFSPSFQNFTISLSFHSNTFN
                     DGPERFLINKGGIGKETPGNNLNFGIWLNEKGHLTGGFETKKGDDFFVVSKNKYEDIN
                     WHKVLLTYDKSLLKLFIDNTLQSEINTKGAIPDDNNLPIIRLGANFLKQNNFYVGSLD
                     NILLWNRSLNANEISSFFTNNTIPSGSVVDVGLSTTNFYNEDKISSLNFNGTNYFDLK
                     VTPNITEGLIEPFHLENTKTPNLKYGPLVYEKSYNYGFMDGFIFAQNDNQTKSKDIQK
                     SDNPTIVMGYYNNSEIRSYWDLASNFVLADNFFGASSQTDLTNNLYLFTAEEGNFKKY
                     VPPRGLSINSTILDLLEKKQITWKVYVENYDSNLNYTNEDDSSKRYLTLNPILGIPRF
                     VDNATLNERIQDLTNYFHDLKNNFPSVSYIILPDSHESSPKDILKGQESVISLIISLM
                     ESKYWKNSVFILTYSGSGGWYDHVPPPINSEYEHGFRVPTLFISPYSHKGTVDSTFYD
                     TLSILKFIEYNFNLNPIDSRDSLSNNILPAFDFNKPPRNSNEIVSKLLENYQSNITNT
                     KPVIKENISFTFNIYLTVLVCIILFFFILAIYYKKSNPSI"
     gene            complement(14763..16295)
                     /locus_tag="VF222_11795"
     CDS             complement(14763..16295)
                     /locus_tag="VF222_11795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7143148.1"
                     /translation="MNFKYFLTSSTNYALLKQEKYVIFSIFVIAFIIRFYGLGDLALN
                     NDEAIYAGQAASLADYSEYQEQFSIFRAHPLLLQFMISIAFYFFGVHEFTARIVPMLL
                     SCGIVVISYFIAKYLYNQQTAIISSIIISLLPYHISISKQALVDVSMSFFFALDLLLI
                     ILYYRKNTVLLLYGIGITTGLCFLSKEIGIISFAISILSLIIINRKVIFKNILVVTLS
                     FIVVASPYWISFITLEEARISILEYIEWQSSRPANHDTFYYFHIITSEILGYVLTSLL
                     LLAYVYVIFSRRNWNFSHVLLPFLWIAIPLIFFQMISVKGYHFLFSITPFLVIFSISF
                     LSRDWIKKISRKEILLIGIIPLVLISNNYIINGYFLFISHNPVLGSESLFHMKDAAMW
                     IKENTPKNSTILTLYTHMSNIIKFYSQRDSIAIQSNNNPSYQKIENIDMPILAKKINY
                     IVYEKIQIDNGKFLENKAIELENYVRKYDGVPVYSNYQNYSYVENNKFLSIPAIIVYE
                     FK"
     gene            complement(16372..16911)
                     /locus_tag="VF222_11800"
     CDS             complement(16372..16911)
                     /locus_tag="VF222_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143149.1"
                     /translation="MRNNEKYLFTILTLATITIVGITIEKAYPQLDDSQLNDSQLNGP
                     AMDSQKTNGETTSSEDKFTPKMYVKITSHKNADKVPAGELTINGTSSDTASRDCIVEV
                     DWNNEKPSQIVKPTGPGGSSDFSTWTFNYTKEYREIEIGPNELTSKLTCINPGPLTKW
                     FSVTLTGEQPPFPRALPIP"
     gene            17017..17190
                     /locus_tag="VF222_11805"
     CDS             17017..17190
                     /locus_tag="VF222_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143150.1"
                     /translation="MNTHSNILLLDVPFHQYKVKKLSTNKDIPIRITTNPLIVQFELL
                     YIKERLGPTKDLL"
     gene            complement(17473..17898)
                     /locus_tag="VF222_11810"
     CDS             complement(17473..17898)
                     /locus_tag="VF222_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7143151.1"
                     /translation="MDIDNHTDEEIKKFYNLKNIAVVGMSKNEEKPSHYVPKYLVQNG
                     YNVIPVNPTTEEILERKSYKLVSEIKEDVDIVDVFRKSDDIIPIVDDLLKKKGIKLIW
                     LQKGIFNQRAEELAKKNGIDFVYNRCMLEEHKRLFSEGS"
     gene            complement(18027..18539)
                     /locus_tag="VF222_11815"
     CDS             complement(18027..18539)
                     /locus_tag="VF222_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7143152.1"
                     /translation="MHSSDDSQSMYIHEIMSKNVVKINSDTSALEISKTMVRRRISSV
                     VIIDNNNKIIGIVTEKDLIKEICAKDLLANTITAGKLMSSPLITISKYSTINDATKLM
                     VEKRIKHLAIHENNDIIGILTTYDLINVLRNKIKSMDLDSNLLDAISMADIPLEEGGF
                     SLPLSEDELK"
     gene            18673..19062
                     /locus_tag="VF222_11820"
     CDS             18673..19062
                     /locus_tag="VF222_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143153.1"
                     /translation="MGRWDNDSGRTPPLRLNQKKIKYIMLGGIVVVAIVVLSVFIPRA
                     GLNIDIVERNDAMHSVKTISVKVNNNSPQEIKDVMVQFGENGKMHSFGNIGPFSAIFI
                     TPEKDELNFDRVTVSANGGNIKTIKFK"
     gene            complement(19074..20015)
                     /locus_tag="VF222_11825"
     CDS             complement(19074..20015)
                     /locus_tag="VF222_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX7143154.1"
                     /translation="MSFFFKDNYFDNKFSYSYHNEPFLLEKLLFRLSKKWIAGYSIDD
                     ALNYAFDANERGLKCIINYLGEDLKNAEMVKKTVIEYKNLINKMKVRGIEGSISIKPT
                     QIGLSLDLNFCLNQLLEITSIAKKNNIFIWIDMESFEYVQPTLTIYQKAIEETKEIGI
                     VLQSNLKRSYSDLMKLLIYRPNIRLVKGAYHEKEEYAYQTKHEIDNNYVEMAKLLFSK
                     NDEIQEKHIFAIATHDSNIIQTSLHLFYLYRLKKEHLQFQFLKGIREKLKIKLLLKGF
                     SVHEYVPYGKNWLPYSIRRLRERKSNIILLMRSLISS"
     gene            20203..21534
                     /gene="thiC"
                     /locus_tag="VF222_11830"
     CDS             20203..21534
                     /gene="thiC"
                     /locus_tag="VF222_11830"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7143155.1"
                     /translation="MTSARRGVPTEEMLRVAKDEDIDINFLIQKVANGSIIIPKNNIR
                     KQEIKVVGIGKGLKTKVNVNIGTSTLYQNLDEEVAKAKVAVKYGGDTIMDLSDGGNLD
                     LIREKLLEAAPITFGTVPIYQAYAYGVEKYKNPLNITEDDFLNAFEKHAKDGVDYTTI
                     HSGITKEIAKRVLEVKRHAGIVSKGGTITAAWMLKYDKENPYFQHFDYMCEIAKKYDV
                     TFSLGDALRPGSILDSHDELQVQEMINVARLSKKAHEYDIQVMVEGPGHVPLNEVAAN
                     VRLAKSLIGEVPYYVLGPLVTDIAAGYDHIASAIGAAVSASEGVDLLCYLTPAEHLSL
                     PNVEDVKAGLIAYRIAAHAGDLVKMRDKAIKWDREITKARRTLNWQKQIELSINPEEA
                     ERIHNRQGQHEGNNVPCTMCGGACVYIMLPQQRKYEDKDEIVSEIKTTTRE"
     gene            21661..21804
                     /locus_tag="VF222_11835"
     CDS             21661..21804
                     /locus_tag="VF222_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143156.1"
                     /translation="MFGKAKCKLCGEKVRFAIRHLKNEHQKEFERLTNLDMPKIMKKY
                     FIE"
     gene            22074..23348
                     /locus_tag="VF222_11840"
     CDS             22074..23348
                     /locus_tag="VF222_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7143157.1"
                     /translation="MKKDNHHFSNLSRMKFDLSEDQKTFLKEIDRICKLLREEEEKSY
                     LKDSLNEKLIPLFNKIGMLGCPISKEYGGLGYDILSYLLSLERIGEEGSSMRTFFSCH
                     TSIGQMILQNWGTDEQKQKFLPNTTKGTDIMAFALTEPNAGSDPSLIETKFEERGDHF
                     ILSGKKHWVGNGTFAKTITTYAKEKGSDKKISAFIIDLNSPAIRIKEIKNKVGLLTVK
                     NAIIEFNNCKIPKENLLGLSGNGLSIAFSALIDGRLSVAAGALGVMKDCLNETIRYSK
                     IRSQHGTVLAKKQLVQQHISKIIVALESTKWLVYKAATIRQQLQIYLESLKSEDKGWI
                     FKINRKNKKYSDLRNECDRFSAIAKYYATNASVDVANRSVQIFGSEGYKKTSRVARHF
                     LDSRATTIYEGANEVLELKIASEILGNEYRAY"
     gene            complement(23695..24555)
                     /locus_tag="VF222_11845"
     CDS             complement(23695..24555)
                     /locus_tag="VF222_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX7143158.1"
                     /translation="MHYSNVANIHESNIVLLGVPDESKSKASRKGTRNGPNQLRKALL
                     ASENFKRSGKIIPICPIKGSLSTKKVLDIGNVQRENLYYQISKLISQSKIPIVIGGDH
                     SITTTTLRAIKNSTKKEINLIYFDAHPDFVTSTNDYYGSVVSDSLDCINIKRSVFIGI
                     RACEPEEVFNIGKYGANIIDPIDIQEYGVKKILQKIIQLCINRSDSNIYISIDLDCLD
                     PAFAPGVSVPTPCGINPLELTFFIQQILKKTKIIGCDIVELSPSFDINNITANFAARL
                     FKEILGSIMV"
     gene            25119..25655
                     /locus_tag="VF222_11850"
     CDS             25119..25655
                     /locus_tag="VF222_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7143159.1"
                     /translation="MFKENIFFSKKKEINKDYFRGEVIMIEVLNKNNSVAHEMYHVFF
                     KKGALTTLHFHETEQILITTNGKGILCLFQENVIENLEASSETIILEDGDVISIPPFI
                     WHFHGSLNNDFAHIALRNTFRIDSSGNQVQAGNVWEKDFINNLSQLNNNESQQLSLKI
                     DKKVKEIVHSEITKSMIS"
     gene            complement(25679..26236)
                     /locus_tag="VF222_11855"
     CDS             complement(25679..26236)
                     /locus_tag="VF222_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143160.1"
                     /translation="MNLKRPVGITIISVLIILNGSFLLFSGIVAFILASNFAAHFDTL
                     DLSPFILSETNNTNFSNNDPNIIKNFNYFIYFIAVTITLFSLIHLVIAYGLLKGKSWA
                     RSTTLILSFMSTIGNILLILIVVSWFPIAGSISSIQSIFVGNILTIIINILIIYYLFK
                     KEVKDYFNFMSSKRSSFTSSFDDLR"
     gene            complement(26411..27985)
                     /locus_tag="VF222_11860"
     CDS             complement(26411..27985)
                     /locus_tag="VF222_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7143161.1"
                     /translation="MQFRNELTYENYIKRNQENIFHNKFDNAVESLMKELNNQDYPVI
                     INNEKIYTRDKYKHVSPVDRRIILGYVSKISDQLGNKAIESSFQSFKEWSKLEYQKRI
                     GILKKAANTIRSNKFPLAALLTFENGKNRYESIADIDEAIDFINYYCIDMINNDGYVT
                     KNKTIARTQKSISVMKPYGVWAIISPFNFPAAIFVGMNIGAIITGNTAVAKPASNTPI
                     IGYKIVEIMIESGLPPGVLNYITGDAAKIGRIIINSNKIAGIVFTGSKDVGYTLYKNS
                     ANVKSRPIIAELGGKNPTIVTETADLDKAAEGVSRAAFSYGGQKCSACSRVYVHKKVK
                     NQFIEKLILNVKKINIEYSDKRNCDLGPLINSNAFQNFKKYVNIASQEGKILYGGKVI
                     EEGNLKYGYYVSPTIVNEVSSNNILLKKELFLPFLCINEFHQFSEALDLCNNSEYGLT
                     AGIYSKNKKEINDFVNNIESGVVYVNRHKSSTTGALVGNQSFGGWKNSGISGKGTGGK
                     YYLTQFMREQSQTIAE"
     gene            28050..29294
                     /locus_tag="VF222_11865"
     CDS             28050..29294
                     /locus_tag="VF222_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7143162.1"
                     /translation="MTHNYKNNHANIMIKKKSIAIVKKIKKLAYNSTFVYPLVIKNGQ
                     GCYLEDIDGKQYLDFTSNIASCPLGYNHPELNEIITRYGGIGAHKIAGQDFYCEEHSQ
                     LAEKLLSISHPNSKVFFINSGAEAVENALKLAYRRSGPLPGISCINAFHGRTLGALAF
                     TFSKEVQKANFPEFPAKRIKFCISDNDPEIDTIQKIVSEYKISFIITELVQGEGGVNV
                     ASKKFVNNLNNTSIKYDIPLIIDEVQTGLGRTGKWWTYQHYGIKPDIVTSAKALQVGA
                     VVFDKKFEPRRPGVLSSTWGGGSRIDMAIGIKTIDIISKGKLLDKVNKSGDFLKKCLS
                     ELKNKHDIIMDVRGLGLMLGVEFHKQEKRDKIINTLFKKKLLVIPSGLKTIRILPPLV
                     ITPDEISKGLNIFEKVLKEFNR"
     gene            complement(29284..29862)
                     /locus_tag="VF222_11870"
     CDS             complement(29284..29862)
                     /locus_tag="VF222_11870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7143163.1"
                     /translation="MLKNAPNGWKVLEDERNVYLNEYIQLYEELLDINGIKKIYVRGK
                     RKNYSTVVPFLSADKILMIKSYRHLVDSLQFEIPSGYIEDGESPKDAAIREFKEETGY
                     SANKILFVGEYTLDYSMFEQKGYIFAAYDLVKTQKQHLGLMESIKIESLTIDQVKEML
                     FKGKILNAASIVALYRSIELHEKNLRKKSFND"
     gene            complement(30426..>30675)
                     /locus_tag="VF222_11875"
     CDS             complement(30426..>30675)
                     /locus_tag="VF222_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7143164.1"
                     /translation="FQDRTMKYDILLRQYLKYFFFNSVGVIIQISIVYGFGKTSIDYG
                     AIIIFAISIASILNYIVNKKFIFKNSKEVVDIRNRFWN"
BASE COUNT        11118 a         4449 c         4529 g        10579 t
ORIGIN      
        1 ggtaattgat cgaaggcatt caataacaga taatatagct agataacttg tttttggatt
       61 agtagaacta ggaatatttt ttacacgtac tattaattcg ccaaattttc ctattacctt
      121 gatttcatgt ttattaatac ggatcgacgg atcagcaatt acattaacct tggttttttc
      181 aacacctata ccagcaagcc ctaataaagc tgctacattt acgttagcag gaaaattctg
      241 aactgcctca atagcagatc cttcaaaaat taatgttctt tttttgatag aatctactgt
      301 caaattagtg tttttaaaaa atggagaatc tttaagtgaa tttggattct tagttgttgt
      361 tagagttaca taacttattg aattttttac actctttaga gcatctattc cagctatggc
      421 tcctgaagga acataaatat taatatcatt cccttcaaca tttcctacaa cttcagagaa
      481 gaaatctgaa ttagcaaaag cacccacact cataacgagt acatctttct tgaataaaat
      541 aagctgattg agatagcttg ttagagcatt ttgagaagca gcttcgacta ctaactcaac
      601 ttctttaaaa gattcagaat tgataaagtc agaaaaattg ttaaagatta cagggttatt
      661 attcgataat tttgatttaa gggtttctgc attatcaggt ctgatatcaa ggagagatag
      721 taacgttaca tttttagcca taatactatc aacagatagt gcaagctcac ttcctatagc
      781 tccacaacct atgattgata tctttttttt cataaaacgt aattaaagat ctttattcca
      841 agagttaaaa tagtttccta aaataatctt taaaataaat ttgtattaaa aaaataagac
      901 ttcaattaca ttgagatcag tttaaaataa aagttatttt gagataacag tcaactgact
      961 agttatagta aatagtagta atcaaatata aaaatgtcat ccaattgata acattctatc
     1021 tattgcagat ttggccttat cagctattga ttttgggact ctaacttgat atacattatt
     1081 tactaaagaa ttgtaaactt tatcaatagt aatttttttc atatactggc agactgcatc
     1141 ttttctaatg ggaatgaaat tttttgttgg gttttctttt tgcatcctat acaatattcc
     1201 tgtttctgta gcgacgataa agtcagtttt agatgaattt ttagcccgtt ccatcatacc
     1261 ttcggtcgat aaaatgtgac cttcatttaa caagtctcct tttgacatat gatacatagt
     1321 tgaagatgta caactgcatt ctggatgaac taagaattct gcatttcgat agtccttcat
     1381 cattttatta atatcttctg cacgtattcc agcatgtaca tgacattcgc ctggccatat
     1441 gaacatgttc tttcttttag taacttcaga tacataagaa cctaagaaca tatctggaag
     1501 aaagagaatt tcattgttgg gagatatact attgaccact tttacagcat tagatgacgt
     1561 acaacaatag tctgataatg ctttaatctc tgcagaagtg ttaacatagc taacaacaat
     1621 agcatctggg tggtcttttt tccaattcaa taattcagta caatttatag aggaagcaag
     1681 agaacatcct gcattgatat ctggtataaa tacatcttta tctggacaaa taatagaagc
     1741 agtttctgcc atgaaatgaa caccacaaaa aataatattt ttagcattag ttttttcagc
     1801 ttttctggag agagctaatg aatctcctgt aaagtcggct acatcttgaa cctcaggtaa
     1861 ttgataatta tgtgcaagaa taattgcatc tcgttctttt tttaaagagg aaatttcttc
     1921 aatgtattga tccatggata aactcatttg cgatttatat acataggagc aaaacaggat
     1981 ttaaattagt actatttagg acacctttgc cttaaatgtg tgtattctag acatagattg
     2041 cctctttaca gattggaatg tatttattag gaacatattt tgtatagaga tatgccaacg
     2101 cacggatcgc ttactaaagc aggaaaagta aagggtcaaa ctcctaaggt tcaaggaaag
     2161 acgagggtta gccctatttc caaagtacgt aacaaagaga atttctttaa aagatttgaa
     2221 aaaagagtcc caccgggtca gaaaaaacct gaacgtggtg gaagaagaag ataaaatcta
     2281 aatccttttt atttttgtca cttgtctatt acccttaaac tgaaatctaa tgcgggagct
     2341 gaatgtgtca aataaccaat tgaaataata ttgggtttta attttgcata attaagtaca
     2401 tttgctaaat ttattccccc tgatatttca attttaacta aatttctcaa accttcatcc
     2461 ataatcttta acattgtttt ttttgcttct agtatagaaa aattatctag cattataaga
     2521 tctgctccag cttcgatagc ttcaatcgct tctcgttcat ttttgacttc acattctatt
     2581 ttgagtgatg atcctatttt ttttctaatt gacggtatta tttttcttat agatttatct
     2641 acagcaatat gattatcttt tatcataacc atatcatcta gcctaattct atgacttatt
     2701 cctcctccta taacaacagc ttttttatca aagagtctca atccagggac agtttttctt
     2761 gtggatgcta ttgaaattga cggatctatt ttatgaacta tctcaacaaa ttttcttgtc
     2821 gaagttgcaa taccgctcat tctcataagt aaatttaatg caattctttc agcttttaat
     2881 acatctttgg ctgttccata gatttccata atattctctt tgggtgagat tttcatacca
     2941 tctaatgtat gaggagttga attgcaattg caaatatcaa atagtagttc tacttcctca
     3001 attccacaga cgattcctat aaaatctgat ttaaaaataa ttttggcagt tactgattta
     3061 ttagaatttt ccaggttttc actggtaata tcccctgtac caacatcttc ctgtaagaaa
     3121 tttaataatg actttcttaa gttaaaatga tcggataatt ttttcattaa gtgaccttaa
     3181 gtgcatattt atgaggtttc gatatttcta gagttttagc aataagtgag gttttggaat
     3241 caaaaacaat tactattcct gaccagtcac cagaaagatc cgtagagtta caaattggac
     3301 aaacctttcc cactgttata gccttacatt ttttacatgc taattctcgg ggcatttgtt
     3361 atttagctac tcctttcttt tcctttggcg attgcggtgt ttgagctttt tctatttctt
     3421 ctttaatcca atcaaatgaa cctaaaaatg gctgtctaca tgtaattcct atctttccca
     3481 tagatgaccc tttacctagg cttacagcag taatccttgc acgcattttg gaaccaattc
     3541 tcaaggaacg agatgattgg ttggcaatta tcataccttg tttaatgtca ctttttagat
     3601 aatcatcggt tatttgagaa agatgtaaaa gtgcatcagt aggtcctatt cttacaaatg
     3661 caccaaaatc agttatttct attatttctc cctctactat ctcttgaatt ttaggaaaaa
     3721 aagtcaatgc tttgaaagtt actttgtgat aagttgcacc gtctcctgta actaatttac
     3781 caactgagtc agcgttggca tcaataatca ttattacata ccccaactct gcactgatca
     3841 tactttcgta tttttctttt agaattccaa tcgcagtatt tcgtaatgaa tcggtaagtc
     3901 tgcttggtgg aattctaact atatcagtca tagaaactat ataaaacatg ttggacaaat
     3961 taggatataa tttatcaatt tatgaacctt ttgtttatat atttgaatag gaactaaaaa
     4021 taactaaaat gaaaatttag ctataattat gcatatcgcc gtcgtaaaaa atcaatattt
     4081 aaatattgcg caagagcttt atttttatta tggttacagt tgatcaggtt cttacatcat
     4141 taaaaaaagt aattgatcct gaattacata aagacattgt ctctatgggt atgataaaag
     4201 aaatggctat taataatgat aaaatatctt tcactttaga gcttactaca cctgcatgtc
     4261 cttttaatag tgaaatagaa caggatgtta gaaatgcgat ttcaaattta ggtgttaaag
     4321 atttagattt aaaggttact gcaaaagtga tggaaggaag ggctatttct ttagatgaac
     4381 tattacctgg ggtaaaaaat atcattgcag ttgctagtgg caaaggaggt gtaggaaaaa
     4441 caacagtttc tgtcaacctt gcaatagctc ttgcaaaaac tggcgcaaaa ataggtctat
     4501 tagacgctga tatttatggt ccaagtgtac cattaatgct tggattaaaa gatactcctc
     4561 aagtagtaga tggtaaaatt cagcctccaa tttcagaagg ggtaaaaatt atttcaatgg
     4621 gtttttttta tgaacaatcc cagcaagcag gaatttatcg tggaccaata atttctggaa
     4681 ttattaaaca atttctcacc gatgtaaatt ggggtgaact tgattatctt attattgatt
     4741 tgcctccagg aacaggtgat gcaccattaa caattgctca gacaattcca atttcgggaa
     4801 ttttagtagt tacaacaccc caagaggttg ctatgaatgt ggcagtcaaa gcaataggga
     4861 tgtttaacaa gttaaatgtt cctataatag gagttataga aaacatgagt tatctcgaat
     4921 gtccacattg caattctgac atacatattt ttggaactgg tgcaggtcag aaaataagtg
     4981 aacaatttaa gattccatat atcggagata tacctttaca tccacagatt atgcagggga
     5041 gtgatagagg caaatctgtt attatatcgg atccagaatc caagcaaaca aaatccttct
     5101 taaaaatttc aaaattagtt gctggaagag taagcgtttt agctgcagaa atgcaagctc
     5161 aagaacaata tgaggaaaaa caagaaaata ccgagaaaaa taatttaaag caagcagaaa
     5221 atgcctctta aagatgagca tgtaaatatt ctcaaaaaac cttttggtga attaataaaa
     5281 caagaagaaa tatctaaaga aaaacttatg tctctggtta aagattctaa atctttaata
     5341 actgtagggg atgctacaac agaaacaatt atttcatttg ggctgatgcc aaatttatcc
     5401 attatagacg gtgtggaaaa acgtattaga agaacagaat caaaaatttt ggatctcaaa
     5461 aactttttta ttactagtac ttctaaattc aaccaatacg aatgtaaaaa tccaaaaggg
     5521 actatatcta aagaagcata tctcactata aaaaaaatac tactaaaagg agaacaagct
     5581 attattttta tagatgggga ggaagatatg cttgccttac ccgtttttgc tcttgctccg
     5641 ataaattcag ttgtctttta tggtcaacct ctagagggat tagtaagcgt aaaaataaat
     5701 gataaaataa aaaataaatc aagggcttta attgatagta ttgggttgga ataattcgct
     5761 gggcatgatg aagtggtggc tgtatgaaga aaacatctaa tgatttgcga taccattttc
     5821 tgacccatat cttgaacaaa taataagctc aggtgttatt tcacgtatca catccacatc
     5881 catccttctc atgatctccg atttttattc catataattt aatacaatct ttacaaatac
     5941 tttgaccctc agcaatatgt gagttacaaa atgaacatac attcctagga ttttgcatta
     6001 caagtatgtt tcctgaattg ccatatagtt tttactggaa aagggacaat acgaaggaag
     6061 aaaatattat ctcattaatt atgacatgaa cattcacatt ttgttagctt tatgtcaaac
     6121 gagcagtcat ggtcaggacc acaaatatca ttaataggaa gatcaactgg acaatcagaa
     6181 tgtcgctctc gcttacagaa aaaagaaatc ttcacacgca tgattagcta aagggacatt
     6241 aatttttaac tatatatata aaaaatagta tcagagatcg atattgaata attgcggagc
     6301 ataaattgat ccatctctta ctttttttac ttttgaaact gaaagataat gcggccaaat
     6361 cgtaaccatt accgctcctt ctggtacacc ttctgggacc tcgattaaaa caatatcttc
     6421 agataaaaca ctgtgttcct tgactaattt tgacttggct aggtcaataa gttccaaagg
     6481 cattccacta ctatacagat aaaccttacc tgaatattct attttcgtat tcataaggtt
     6541 aataattaaa taaatgtata ataaattgtt ataaaaaaac attcttaatg aatgtttcac
     6601 aatacaacat cttgcttatt cactcatctt taaagttcat caatatgata ttttgacata
     6661 catcctttac attcatatat ttcatcacca acaggacctg atacattgaa tctaactatg
     6721 gtagtacatt tcggacatcg accttccaca cttctagttt ttctttgagg tttaatagta
     6781 cgtctttttc tactgcccat ctattttatt gtaattcata tactgattta taaacgtctt
     6841 taaataattt tatgaaacct catgagatat tatacaacta ttacattttt aatcggactt
     6901 tataaattag tttagattaa ataaagatta caactactga atctttatca ttgacactac
     6961 caaaaaaatg ctctttttct aatcaagata cacaatgcga aatcccccca tcttacctct
     7021 tatcagttac agaaaatgga gaatatctga ttggtgtatc atgcatggaa cacttggaac
     7081 cgataaaact taagataaaa aaattacaga taaataaaac cattccaaat ggaaaattaa
     7141 aggtagagaa tataaaaatg ttatctacta attgtataaa aggatcacaa gaagattatg
     7201 aagacgttta ttctcaacgt ttaagagaaa attaataaaa tggtaaagca ccggaattta
     7261 actaagtttt ttgttcaagt tctttattaa taagaaacat tggttttttg ttagagagta
     7321 tattgatcaa attaatagcc gcaacttcag acatttgatt tcgagtttgt acagttgcac
     7381 tgccaagatg aggtaaaagg acgacatttt ttaattttat tagtttatta tgttttggta
     7441 gtggttctat ttcaaataca tccaaacctg ctccagcaat ccatttgttt ttcaatgctt
     7501 ttattaaaga agtttgattt attatacttc ctcttgaagt attaattaga aatgactttt
     7561 ttttcatttt ttttagttct ttagtatcaa ttaaatgaaa attatccttt tcaaagcttg
     7621 tgtgaatgga tagaaaatca ctattttgta gtaattgatc aaaattcata aatgttgcat
     7681 ttagtttttc ttctattgat ttagataatt ggtgtctatt attatatatt atcttcatat
     7741 tgaaaccttt agctcgttta gcaacagcag cccctatatt tcccaatcca agaattccca
     7801 aagttgagcc ataaatatta ctcccaagaa ataaatcagg tgtccagcct tttttccatt
     7861 ttccattaat aatatataag tgaccttcta caattttacg agataatgcc agtatcaacg
     7921 agaaagttag gtcagcggta gcttcagaca atatatttcc ggtattagta acataaattc
     7981 ctcttttagt agcctcacgt atatcgatat gatcataccc cgtactgaag gtactaatta
     8041 cctttaaatt agaaccagcg gcatccatta tatcagagtc aattctttca gataaagtgc
     8101 aaagaatccc gtaaacattc ttaacttttt ggatcattag atttttagta ggagattcgg
     8161 ttgtatcagt caatatgaca tcataataat tttttaataa ttctattcct ataggattga
     8221 tttttcttgt tataaaaatt ttctttcttt ccatatagaa tttataaaac tagtttattt
     8281 attctataac aagatatttc aatgctatga aataaaaaaa acattattac tctcaagaat
     8341 atattaagtt agctcattga aaagagggac agtaaatctt cctttacacg gtggtcatgc
     8401 tccttcttat ttagtaaaga ggatgataaa actatcgtca gcaatttgca ctataattat
     8461 agaagaaaat ggcacaaaag aattttttaa acgaatctct gatcctcttt ggtttcaagc
     8521 atttggatgt gtattaggct ttgattggca ttcttctggt ttaactactg tagttactgg
     8581 cgtattgaaa caatcactag atcctgaaaa ccatggaatt agtatttcag gtggcaaagg
     8641 aagaaattct acaaagccaa aatatgaaat tcctcagctt gctgaaaaaa atcacaattt
     8701 atctacaaag gaaattaata atttattata tgcaagtaag atgtcagcaa aaatagacaa
     8761 tgctgctatt caagatggat tcaccttata ccaccatgta attttctttg atgaatatgg
     8821 taattggaca attatacaac aaggactaaa tgaaaaatat aaaatggcta gaagatatca
     8881 ttggatatct gatgacttaa tcaactatat tattgagcct cacaaaggaa tcataggaaa
     8941 tgaaagaatt ccactatgtt taaatatgac ttcaaaagaa tccgaggaaa atcgcaaaaa
     9001 ttgtgtagat ttgataaaag aaggatcaag ttcaataaaa tcctctatct ataaaattag
     9061 ggaaacaaaa acctttgaga acttagatag atggatccac aatagtagat tacaacataa
     9121 taataaaatg tgcttggaac caagactttc caatattgaa aaatatgaga tgcctcgaac
     9181 cattagttgg aagacaatag acgacttgta tgatattact ccaacaaatt acgaagatct
     9241 aatatcgcag aagggagttg gtccatcaac catacgagct ttatctctca tatctgaaat
     9301 catttatggg aatagagcat cgtggaaaga ccctgtcaaa tataattatg cccatggagg
     9361 caaagatgga ctcccatatc ccgtcgcccg aaatacatat gataaatcga taaaatattt
     9421 gagtgaagta ataggtggat ctaaaataga aaaacaagaa aaaatctcat cactaaaaag
     9481 attatctact tattctaata ttctatttaa tacaaaagag tacacttata ctgtgaagta
     9541 aatagttgat ttttactttc gtttaaaata atttttatga tccaaaaatt cgtactttaa
     9601 aattttaatt attaattatt ataaaaaagt agaggaaact tttataagaa atgttttctc
     9661 taatacatcg aaaagatatt gaaaaacata ttattaattg taacggtcac ggcattattt
     9721 ggaacaattt tgggttcgag tattgaaaat tatatttatg ctcaagaagg tgcaattact
     9781 ccagagggtt caatcaatga cactatgtca ggtgaaaacg caactgaatg ggctaatgcg
     9841 acattagcgt ttgctcaaga aggttttacc actgaagatt taattaacga aaccatagac
     9901 ttgggaaatt cttcaacatc taatgcgaca ttagcgtttg ctcaagaagg tgcaattact
     9961 ccagagggtt caatcaatga cactatgtca ggtgaaaacg caactgaatg ggctaatgcg
    10021 acattggctg aaagttggga tattaaataa ctcatttttt cttttactgt taaattcatt
    10081 ttgttaaatt aacaaaatta tagtgccccc ttcagactag aatactattt gaccttatag
    10141 ttcttattta acgaatattt agtttcataa tttaccgaaa gggattgtga attaatgtgt
    10201 tagattcact tttcacttat aaaaaagata aatttatttt agtagatcct accaaattct
    10261 tattctatct atacttaaaa cgtcctacaa tatgatctag ataactttat agaacatata
    10321 gctccaaatt actaagaaat accaaattaa tatagcaaag gtttagtaat taataaaaat
    10381 aattcacatt ttaggttttt gaaaactatc tgtctttctt aaacagacgt aaacacaata
    10441 aaccataaga aatactgtat tatattcata aaataaatga atttattcaa tagaatccaa
    10501 ataacagatc tttttaaagc ttaaattgtt aattttatat ctaccaaatt agttctatga
    10561 tataatattt attttacttg ataataagaa gaaccatatt tgataaaaat atggatgagt
    10621 cctaatggaa atctttgata atatatctcc cggcaatcaa ttggctaaaa atatttatgg
    10681 aacctgtcat ccatttataa aatgggcagg aggaaaatca cgtttaattt ctcaaataaa
    10741 aaattttatc cccaaagaat ttaataatta ttttgaacct ttcataggaa gtggcgcatt
    10801 atttttttat ctggtaagtt cttttaaaat taaagggaat tcctcagcac atctatctga
    10861 tattaaccct gaattgataa atgtatataa aaccataaaa gataacttaa aagatcttat
    10921 taaagaatta caatacaatg aaatcgaata ttataaggat tcaaaaaaat tttattatga
    10981 attacgagat gaaaattttg ggttcaatag tattaagaaa gcagctaggt ttattacctt
    11041 aaataagaca tgttataatg gtttatacag agtcaataag aagggtttgt ttaatgttcc
    11101 aattggaagt tatataaatc caaaaatttg tgatcaggaa aagctaaata tagtaagtaa
    11161 aattctgaat ttaacaaata caattataga aaattacgat taccagaagg ccattataaa
    11221 aatcaaagaa aatgatttta tatatttaga tcccccctat taccccttaa ataaaacttc
    11281 aaattttaca aattatacta gttatggatt tgattatatg gaacaaaaaa gattagctga
    11341 tttttttaat gaattagata aaaaaaaatg taaaataata ttaagcaatt ccgatactgc
    11401 atttattaga gatttgtatt catcattcaa ggagaataca atatctctca atacccttcg
    11461 atcgattaat agtaacgcag ataaaagaaa aaaccattct gaattgatta taaagaactt
    11521 ttaaataatt aagaaaaaag cgaaccggct aacttttaaa tatatgaaat tttctctaaa
    11581 ttaaatgaaa gataaaaaaa tttttaatgg agttaaaata atatacattt cacttattat
    11641 tttaactttt ataggtttct ttaataatgc ttttgctttt ttccatgacc aaggttcaca
    11701 tacaaataga gttattgagg aatcaaaaaa aattatagaa tcattattta atagagtaaa
    11761 tatcgcctcc aacaattatc aaaatgaaat cgcaattttg aacctatcaa tgaataataa
    11821 tgtaacaaag gtaaataaaa ctcaagaata tattgataat ctaaaagaga taatatcctc
    11881 tgctaacaaa accacagtac aacaagatta taaacctgtt ctcaataact atattgattc
    11941 aattcaaaac gaattggaga gctacgtcca ttataataag tatcttataa ctggcaatct
    12001 aactgaaaat caaatatcaa taaaccttct ttcaaaggca tttaataatg aaacacaagc
    12061 aattaacgaa tataaaaaac tggaattaca gtagatttat tttatatatt ttaaatttca
    12121 acaacagcat agacacaagt atcaatttta aaaaacgatt taacttattg atttttatat
    12181 cttaaattca ttcataagaa tatgaattgt ccttgttgtg gaggaaaaat ggaagcaaac
    12241 caatttgata aattaacagt ttttaaatgt aatagttgtg gtttaagtaa tactgaactt
    12301 aagacagata ttaaatgata aattatagtt ctaattagaa attataatta tttccgaatg
    12361 tgacttgaca aaatttgaat aaaggaatca tcaattttaa gaagcgaaaa ttaagaattt
    12421 ttatttatgt atttatttaa ttattcagtc aaagtaaatt ttaaaatggc aacaaaagat
    12481 ataattaatt ttttctgatg ttctaaatgg atcgaaagga gttaaaataa taagttacta
    12541 aacttgattt aaataccatt taaatgtatc aacatacttt taatagaaaa tacctttttt
    12601 taaattatcc aatttattat aattgttata taatacctgg tgtgggtaat gagttttatc
    12661 aaatagcagc atgtaataac aatacataat ctagaagtga tgttatatgg aaggatttga
    12721 ttttttgtaa taaatcgcaa gaataaaaaa aaataatata atacatacta atactgtcaa
    12781 ataaatatta aatgtaaaag aaatattttc cttaataaca ggtttagtgt tggttatatt
    12841 tgattgataa ttttctagta gtttggatac aatttcattt gaatttcgtg gtggtttatt
    12901 aaaatcaaaa gcgggtaaaa tattattaga tagtgaatct cgactgtcga tggggtttag
    12961 attaaaatta tattctatga attttaaaat tgatagggtg tcatagaaag tagaatctac
    13021 cgtacccttg tgagaatagg gagaaatgaa tagtgttggt accctaaaac catgttcata
    13081 ttctgaattt atcggaggag gaacatggtc ataccaacca ccagaaccac tataagttaa
    13141 gataaaaaca ctatttttcc agtatttact ttccattaaa gaaataatta aagaaataac
    13201 tgattcttgg cccttcaaaa tatcttttgg actgctttca tgactatctg gtaaaataat
    13261 ataggagaca gaggggaaat tattcttaag atcatggaaa tagttagtca aatcttgtat
    13321 cctttcgttg agtgtagcat tgtccacaaa tctagggatt ccaaggatag gattcaaagt
    13381 aagataacgt ttggaagagt catcctcatt ggtataattt agattagaat catagttttc
    13441 gacataaact ttccatgtta tttgtttttt ttctaataaa tctaatattg ttgaattaat
    13501 agataagcct cttggaggca catatttttt aaaattacct tcttctgcag taaataaata
    13561 caaattattt gttaagtcag tttgtgagga agcaccaaaa aaattatctg ctagaacaaa
    13621 gtttgaagca aggtcccaat atgagcgaat ttcactattg ttataatatc ccattacaat
    13681 agtaggattg tcagactttt gaatatcctt tgatttagtc tggttatcat tttgagcaaa
    13741 aataaaccca tccataaacc cataattgta agatttctca taaactagcg gtccatattt
    13801 taaatttggt gttttggtat tttctaaatg aaatggttct atcaatcctt ccgtgatatt
    13861 gggggttact tttaaatcaa aataattagt cccattgaaa tttaaagaag aaattttatc
    13921 ctcattataa aaattagtag tacttaaccc gacgtctaca actgagcctg agggaatagt
    13981 attattcgta aaaaatgagg aaatttcatt tgcatttaat gatctattcc agagtaaaat
    14041 gttatctaga ctaccaacat aaaaattatt ttgttttaaa aaatttgctc ctaatctaat
    14101 aatgggcaag ttattgtcat ctggaattgc tcctttagta tttatctcgg attgcaaagt
    14161 attatctata aataatttta ataatgactt gtcatatgtt aataaaactt tatgccaatt
    14221 aatgtcctca tatttatttt ttgatactac aaaaaaatca tctcctttct ttgtttcgaa
    14281 acctcctgtt aaatggcctt tttcattaag ccatattcca aagtttaaat tatttccggg
    14341 agtttctttt cctattcctc ccttgtttat aagaaatctt tctgggccat cattaaaagt
    14401 attactatga aaactcaatg atatagtaaa attttggaag gaaggtgaga attcaaaagg
    14461 gtttattggt atacccatat ttttaggaaa gcccttagtt ccttcatagc ttccaaaata
    14521 attatcaaag cttctccgtc cctgagaaat aacaatcaca tgatcaatag gtgttttatt
    14581 cttttgagaa aattcagtat tttcatgaga atagactgtt gtaataacaa aaacaatacc
    14641 actaattgat aaggagaaaa aaaccaacag tatgaaataa agagctttaa atatcatttt
    14701 agatcctgga tatcattcat taaataattg tcttttatta aatctaaatg aagatcatat
    14761 catcatttaa attcatatac tattatcgct ggaattgata aaaatttatt attttctaca
    14821 taactgtaat tttggtaatt agaataaact ggaactccat catactttct gacgtaattt
    14881 tcaagctcta ttgctttatt ttctaaaaat ttcccattat caatttgaat tttttcataa
    14941 acaatatagt ttattttctt agccaagatc ggcatatcta tattttcaat tttttgatag
    15001 gatggattat tatttgattg aattgctatt gaatctcttt gcgaataaaa ttttattata
    15061 ttggacatgt gagtatacag agtgagaata gtcgaattct tcggggtatt ttctttaatc
    15121 cacatagccg catctttcat atgaaatagg gattctgaac ccaagacagg attatgggaa
    15181 ataaacaaaa aatatccatt gatgatataa ttatttgaaa ttaataccaa tggaattatc
    15241 ccaattagca aaatctcctt ccttgaaatc ttcttaatcc aatctcttga aagaaaggaa
    15301 atactgaaaa taactagaaa tggtgtaatc gaaaaaagaa aatggtatcc tttaaccgaa
    15361 atcatttgaa aaaaaattag gggaatagca atccatagaa aaggaagcaa gacatgcgaa
    15421 aagttccaat tccttctgct aaatatgaca tatacatacg caagtaaaag taacgaagtt
    15481 aaaacatagc ctaaaatttc gctggttata atatgaaaat aataaaaagt atcatgattt
    15541 gctggtctag agctttgcca ttcaatatat tctaatatag atatacgtgc ttcttctaaa
    15601 gtaataaaag aaatccaata tggagaagct acaacaataa aagataaagt aacaacgagt
    15661 atatttttaa atattacttt cctatttata ataattaagc ttagaataga aatagcaaaa
    15721 gagataatgc caatttcctt ggaaagaaag caaagaccag tggtgattcc tattccatac
    15781 agaagtaaga ctgtattttt ccgataatat aagataatta ataaaagatc aagagcaaag
    15841 aaaaatgaca ttgaaacatc aacgagtgcc tgtttggata ttgatatatg atatggaagc
    15901 aaactaatga taatcgaaga tattatggcc gtttgttggt tatacagata tttagcaata
    15961 aaataagata taacaactat accacaactc aaaagcatag gaacgatccg ggcagtaaat
    16021 tcatgtaccc caaaaaaata aaatgcaata gaaatcatga attgcagtaa taaggggtga
    16081 gctctgaata tagaaaattg ttcctgatat tcactataat cggcaagcga tgctgcctgt
    16141 ccagcatata ttgcttcatc attattaagt gctaaatcac ccaaaccata aaatctgatg
    16201 atgaaagcaa taacaaaaat ggagaatata acgtattttt cttgttttaa aagagcataa
    16261 ttagtgctcg aagttaagaa atatttaaaa ttcaatataa tgttatctag agcagcaaaa
    16321 ttgatttata tttttttcat catgatagat agagaatata ctaaaaaaat tttaaggaat
    16381 aggtaaagct ctcggaaatg gtggttgttc ccctgttaat gtaacactga accattttgt
    16441 taggggtcca gggttgatac aagttaattt agaagttagc tcatttggcc ctatttctat
    16501 ttctcgatat tcttttgtat aattaaatgt ccatgtcgag aaatcactag agccacctgg
    16561 tcctgtaggc ttgactattt gagatggttt ttcattattc caatctactt ctactatgca
    16621 atccctagag gctgtatcac ttgatgttcc attgatagta agttcgcccg caggaacttt
    16681 gtcagcattt ttatgagatg taattttcac atacattttt ggagtaaatt tatcttctga
    16741 tgatgttgtt tctccattgg ttttttgaga gtccattgca ggaccattca attgtgaatc
    16801 gttcaattgt gaatcgtcca attgaggata tgctttttct atagtaattc ccactatagt
    16861 aatagttgct aatgttaaaa tcgtaaagag gtatttttca ttatttctca ataataattc
    16921 aacataatac gggttcatta actttgaaat taagaatgtt tatttaaaaa gtaatgtaaa
    16981 aatggataga gcagctgcta aagatatatt cgctctttga atacacattc aaatatttta
    17041 ttgttggatg taccttttca tcaatataaa gtaaaaaaac ttagtactaa caaagatatt
    17101 cctataagaa ttacaacaaa tccattaata gtccaatttg aattattgta cataaaagag
    17161 cgtttaggtc ctactaaaga tttgctttga aggtaaaata taattccaaa aaaaagaata
    17221 attagaccta aaattatcaa atataatatc attacttaca ctatataaaa aaataggatt
    17281 agaataataa tcctttccat atatatagaa tatttaacta taagacgttc gaattatcag
    17341 tgttttgaat tgagaattaa ttattagtat ttatatataa ttaaatgggt aagattgaaa
    17401 actggcacag gctactatat taacatataa gatgttcttg cagtcatggt gtgatcaaca
    17461 agcgtttaac attcaacttc cttctgaaaa cagccttttg tgttcttcta acatacatct
    17521 attatataca aaatcgattc catttttttt tgccaattct tctgcacgtt gattaaaaat
    17581 tcctttttgt aaccatatta actttattcc ctttttcttt aataaatcat ctactattgg
    17641 gataatatca tctgattttc gaaatacatc tacaatatca acatcttctt taatttcgga
    17701 aacaagtttg taagattttc tttctaggat ttcctcagtt gtaggattta ctggtataac
    17761 attataacca ttttgaacta aatatttagg gacataatga gaaggctttt cttcattttt
    17821 ggacatacct actactgcta tattttttaa attatagaat ttttttattt cttcgtctgt
    17881 gtgattatct atatccatta tttataatat gtattaaagt atttattaat taataaagtc
    17941 tgggtataac gtttttatta attatagaag aggatataaa tttataaaga aaaatgaacc
    18001 attaactttg tttttattta ttctcattat tttaattcat cttcagataa tggaagggaa
    18061 aatccacctt cttctaaagg aatatcagcc attgaaattg catctaatag attagagtct
    18121 aaatccatag atttaatttt atttcttaaa acattgatga gatcatatgt ggtaagtatt
    18181 ccaatgatat cattattctc atgaattgcc aaatgtttta ttcttttttc taccattagt
    18241 tttgttgcat catttattgt agaatatttg ctgattgtta tcaaaggaga agacattaat
    18301 ttacctgccg ttattgtatt tgcaagtaga tcttttgcac atatttcttt tattaggtct
    18361 ttctctgtaa ctattcctat aatcttgtta ttgttatcga taataaccac tgaactaatt
    18421 ctccttctta ccatggtttt tgatatttct aaggctgatg tatcagaatt tatctttacg
    18481 acatttttac tcatgatttc atgaatatac attgattgag agtcatcgga actatgcatc
    18541 aatatgaatt taaaaaggtt attaattaaa tttgtttact gaaaacaaat taataatgac
    18601 ataaaaaaga aaaagattga ataactatga tttaaacatt cactttatta taatgtaata
    18661 atatatcata atgtgggaag atgggacaat gactcaggca gaacgccacc tctaagacta
    18721 aatcagaaaa aaataaaata tattatgctc ggcggtattg tagttgttgc gattgttgtt
    18781 ttatcagttt ttattccaag agcaggccta aacatagata ttgttgaaag aaacgatgcg
    18841 atgcattcag tcaaaacaat aagtgtaaag gtaaacaata acagtcccca ggaaatcaaa
    18901 gatgttatgg tacaatttgg tgaaaacggc aaaatgcatt cttttggaaa tatcggacca
    18961 ttcagtgcaa tatttataac acccgaaaag gatgaattga attttgatag agttactgta
    19021 tctgcaaacg gtggaaatat taagactata aaatttaaat aattaatgtt taatcaagag
    19081 gaaattaatg aacgcattaa tagaattata ttactttttc gttctcgtag tcttctaatg
    19141 gaataaggaa gccaattttt accgtaaggt acgtattcat gaacactaaa tccttttaat
    19201 aataatttaa tttttagttt ttcacgaatt ccctttaaaa attggaattg taaatgttct
    19261 ttttttaaac gatataaata aaatagatgt aaggatgttt gaattatatt agaatcatga
    19321 gttgcaatcg caaaaatatg cttttcttga atctcatcat ttttcgaaaa taagagtttc
    19381 gccatttcga cataattatt atctatttca tgtttcgttt ggtatgcata ttcctctttt
    19441 tcatgataag cgccttttac taatcttata ttaggacggt atattaataa tttcatcaaa
    19501 tcagaataac ttctttttaa attggattgt agtactatcc ctatctcctt tgtttcttca
    19561 attgcctttt gataaattgt caaagtaggt tgaacgtact cgaacgattc catatctatc
    19621 caaataaaaa tattattttt ttttgcaatt gaggttattt ctaataattg attgagacag
    19681 aaatttagat ccaaggagag accaatttgt gtgggtttta ttgaaattga tccttcaata
    19741 cctcttactt tcattttgtt tatgagattt ttatattcta ttacagtttt ttttaccatt
    19801 tctgcatttt ttaaatcctc tccgaggtaa ttaattatac attttaaacc tctttcattt
    19861 gcatcaaatg cataatttaa cgcatcatct atgctatatc cagcaatcca tttcttagat
    19921 aatctaaaaa gaagtttttc taataggaaa ggttcattat gatacgaata agaaaattta
    19981 ttgtcgaaat agttgtcttt gaaaaaaaaa ctcatgatat attttataac ctatttttat
    20041 taaagttatt ttttgtaatg gatgcataat agtatatgta agtttacaat taatggacta
    20101 aattgtgatt gaatggtaat ttcaaaacct tcgctagaca agaaagagac attcttttat
    20161 attcatttgt tctatattta atagaataaa atgtgcaccc aaatgaccag tgcaagaaga
    20221 ggggttccga cagaggaaat gctccgggtt gcaaaagatg aagatataga tataaatttt
    20281 ttaattcaga aagtagcaaa tggttctatt attataccca aaaataatat aagaaaacaa
    20341 gaaataaaag ttgttggcat tggaaaggga ctaaaaacca aagttaatgt gaatattgga
    20401 acatccaccc tctatcaaaa cttagacgaa gaagttgcaa aggccaaagt cgcagtcaaa
    20461 tatggtggag atactataat ggatctctct gatggtggaa atttggattt gatccgggaa
    20521 aaacttttag aagcagcacc aattactttt ggaactgttc ctatttatca agcctatgcc
    20581 tatggtgtcg aaaaatacaa aaatccttta aatataactg aagatgattt tttgaatgca
    20641 tttgaaaaac acgccaaaga tggggtagat tacactacca ttcattcagg tataacaaaa
    20701 gaaatagcaa aaagagtttt agaagtgaaa agacatgcag gtattgtatc taaaggagga
    20761 acaatcacag ctgcctggat gttaaaatat gataaagaaa acccatattt tcaacacttt
    20821 gattatatgt gcgaaatagc taaaaaatat gatgtaactt ttagcttagg agatgcttta
    20881 agaccaggat cgatattaga ttcccatgat gagctccaag tacaagaaat gataaatgta
    20941 gctagattat ccaagaaagc acatgaatat gatatacagg ttatggttga aggacctggt
    21001 catgttccat taaatgaagt ggcggcaaat gttagattgg caaaatcact aattggagaa
    21061 gttccctatt atgtacttgg cccattagtt accgatattg cagcaggata cgatcatatt
    21121 gcaagtgcaa taggcgctgc agtatcagct tcagaaggtg ttgatctttt atgttattta
    21181 accccagcag aacatctctc tctgccaaat gtcgaagatg ttaaagcagg attgatagca
    21241 tatagaattg ctgctcatgc cggtgattta gtaaaaatga gagacaaagc gatcaaatgg
    21301 gatagagaaa taacaaaagc aaggagaacc ttaaattggc aaaaacaaat agaactttct
    21361 ataaatccag aagaagccga gaggattcat aatagacaag gccaacatga aggaaataat
    21421 gttccatgta caatgtgtgg aggagcttgt gtatatatta tgttaccaca acaaagaaaa
    21481 tatgaagata aagatgaaat tgttagtgaa ataaaaacaa ccacaagaga ataataatga
    21541 tttagaatag aaaataccta aactcattta cgaacaataa tgtattttta atctcaatgt
    21601 atcttaattt tagataacat aataacaaaa gttaagtatt gcatatcaag ctgatctatc
    21661 atgtttggaa aggccaaatg caaattatgt ggtgaaaagg ttagatttgc tatacgccat
    21721 ctaaaaaatg aacatcaaaa agaatttgaa aggttaacca atctagacat gccgaaaata
    21781 atgaaaaaat attttataga ataaaagatt acacctacta tcatgtatca tagtatttct
    21841 gatatgaaac ttttgatgtt cagcccgaaa aagttagaat tcgttaatac aatccctggt
    21901 actttcattc cagcagcctt ttaccaaatc ttcaattatg ttgtaactaa gaggtatagt
    21961 ttagatcttt caaattaaga tttataatag atttctttaa taaagtgaca aatttttcta
    22021 ttattattgt ttaaaacatt tatttttatt aattgtaata tacattttgt gaaatgaaaa
    22081 aggacaatca tcatttttct aacctttccc gaatgaaatt tgacttatct gaagatcaga
    22141 aaactttttt aaaagaaatc gaccgtattt gcaaattatt acgagaagag gaggaaaaaa
    22201 gctaccttaa agactcgctt aatgaaaagc ttattcctct tttcaataaa ataggaatgc
    22261 ttggttgccc tatctcaaaa gaatatggtg gcttaggata tgatatttta agttatttac
    22321 tttcattaga aagaattggt gaagaaggaa gttccatgag aacatttttt tcatgtcata
    22381 catctattgg acaaatgata ttacaaaact ggggaactga cgaacaaaaa caaaaattcc
    22441 tgcctaatac tactaaaggc acagatataa tggcttttgc tttgacagaa cctaatgctg
    22501 gaagtgatcc ttctttaata gaaacaaaat ttgaggaaag aggagatcac ttcattttat
    22561 ctggaaaaaa acactgggta ggtaatggaa cattcgcaaa aacaataact acatatgcta
    22621 aagaaaaagg aagcgataag aaaatttctg catttattat agatttaaat tcaccagcaa
    22681 taaggataaa agagattaaa aataaagttg gactcttaac tgtcaagaat gctataatag
    22741 aattcaataa ttgtaaaatt ccaaaagaaa atttgcttgg attatctggt aatggattaa
    22801 gcatagcatt tagcgctttg atagatggta gattgagtgt tgctgcaggt gcactcggag
    22861 tgatgaaaga ttgtttaaat gagactataa gatattctaa aattagaagt caacatggaa
    22921 cggtattagc aaagaaacaa ttggtgcaac aacacatctc taaaataatt gtagctttag
    22981 agagcacaaa atggcttgtt tataaagctg caacaataag acaacaactt cagatttact
    23041 tggaaagttt aaaaagtgaa gataagggat ggattttcaa aattaatcga aaaaacaaaa
    23101 aatattctga tctaaggaat gaatgcgatc gattttcagc aattgccaaa tattatgcta
    23161 caaacgcttc tgttgatgtt gcaaatagat cagtacaaat atttggttcg gaaggttata
    23221 aaaagacgtc aagggttgca aggcatttcc ttgattcgag agcaacaaca atatacgaag
    23281 gagcaaatga agttttagaa ttaaaaatcg ccagtgaaat tcttggaaat gagtatagag
    23341 catattaaat agcattttaa tcaatattca actttatcga ctttaagaaa ttgttaagac
    23401 tgatcatata agatactatg ttaaagtaga gagcaaatta gataaagaga tacatacgca
    23461 accttcatca gttgttcaag ggatcaaatg taactcatat ttttaaatac catcgaattg
    23521 gtatataatt gttttttata tttatctaag gcctacaata ctgtagtata gattaaataa
    23581 atagggtatt cattatagaa catattaatt atttactaaa aaatgtccat cttcaacttt
    23641 ctttatattt gagaaaagaa ttaatgtaag tttgttacta atataaagtt tagattatac
    23701 cataatagaa cctaaaattt ctttaaataa tcgagcagca aagttagcag taatattatt
    23761 aatatcaaaa gaaggagaca attcgacaat atcacaccca attattttag ttttttttaa
    23821 tatttgttgg atgaaaaaag tcaactctag tggattaatt ccacatggag tgggtactga
    23881 aactcctggt gcaaaagcag gatctaaaca gtctaaatct atggaaatat aaatattact
    23941 atcggacctg tttatacata attgaataat cttttggaga atttttttta ctccatattc
    24001 ttgaatgtca attggatcaa taatatttgc tccatatttc ccaatattaa atacctcttc
    24061 tggttcacat gcgcgaatac ctataaacac acttctctta atatttatac aatcaagaga
    24121 atcactgaca actgatccat agtaatcgtt agtagatgtg acaaagtcag gatgagcatc
    24181 aaaataaatc aaattaattt cttttttagt actgtttttt attgcacgta aggtagtggt
    24241 agttatggaa tgatctcctc ctataacaat cggaatttta ctttgagaaa taagtttaga
    24301 aatttgataa tataaatttt ccctttgtac atttcctata tctaaaactt tttttgtaga
    24361 taagctcccc tttattggac atattggtat gattttaccg gagcgcttaa aattctctga
    24421 tgccaatagc gcttttcgta attgattagg tccatttctt gttccttttc tcgacgcctt
    24481 agattttgat tcatcaggta ctcccaataa tacaatattt gattcatgaa tattagcaac
    24541 attagaataa tgcatatcaa ctcaatgatc aatgatcatt atttcaactt tttgattata
    24601 aattataata aactggcgga gtaatcattc tttaccacta gaagtataac ctgtgccgat
    24661 ggattataat tatatatcta tatcattcta tcaattatat ataagttctc atcaaggata
    24721 cgatcattat aatacaaaga aagtgttaac aatttataat ttgtaactta ttcaaacaaa
    24781 actatttcct taatctttaa tcaatccaaa atataaacaa aataagtatt ataaatcact
    24841 tacatatttt ataaaattcg attataataa tagtttcatt tactcataca atattactta
    24901 attatttata tagtttaggt ttaagttaat attattctca ttttcaaaag taatttggtt
    24961 ttgattgatt gcgatagata gaataaacta aagtaattat atatctaata ttcgtaggaa
    25021 aattataagt atcaagatta tttagtcatt attatatttt actttactat tttaatataa
    25081 ggcttagaaa tatataatta aaaatcaagg ttagaacatt gtttaaagaa aatatatttt
    25141 tctcaaaaaa aaaagagatc aataaggact attttagagg agaggtaatc atgattgaag
    25201 ttttaaataa aaataattct gttgcgcatg aaatgtacca tgtttttttt aaaaaaggtg
    25261 ctttaactac tttacatttt catgaaactg aacaaattct gattacgaca aatggaaaag
    25321 gaattttgtg tctttttcaa gaaaatgtta ttgaaaatct tgaggcatct tcggaaacaa
    25381 ttatacttga ggatggagat gttattagta taccaccatt tatttggcat tttcacggtt
    25441 cattaaataa tgattttgct catattgcac taagaaatac gtttagaata gattcaagtg
    25501 gcaaccaagt acaagcagga aatgtatggg aaaaagattt tattaataat ttatcacagt
    25561 taaataataa tgagagtcaa caactctcat taaagattga taagaaagta aaagaaatag
    25621 ttcatagtga gataacgaaa tcaatgatta gctaatcaag aaatcatcac ttatttattt
    25681 accgtaagtc atcaaaagat gaggtaaagg atgatctttt gctagacata aagttgaaat
    25741 aatctttaac ttctttttta aaaagataat atattattaa aatatttatt attatagtca
    25801 aaatatttcc aacgaatata gattgaatac tagaaattga tccagctata gggaaccaac
    25861 ttaccactat cagaatcaat agaatgttac ctattgttga cataaaagat agtattagag
    25921 tagtactacg tgcccacgac ttgcctttta aaagcccgta agctataact aaatgaatta
    25981 aggaaaatag agttatagta acagctataa aataaataaa gtaattaaaa tttttgataa
    26041 tgttaggatc attattacta aaattagtat tatttgtttc cgataatata aaaggactca
    26101 gatctaaggt atcaaaatga gcagcaaaat ttgaggcaag aataaaagcc actattccac
    26161 tgaagagtag gaatgaaccg ttgagaatta tcaagacaga aattatcgtt attcctacag
    26221 gtctttttag attcattaga attttgttga ataccacgat ttataagtat atattttaac
    26281 atgatccaaa gaaaaaagat aataataagc caataacaac aacaacaaca ccaacaataa
    26341 ctctagtaga gataagatat caaaaacgca tcaattatcc ataacaaatc aaaatattaa
    26401 aaattattat ttattctgct attgtctgac tttgttcccg cataaattgt gtaagataat
    26461 atttccctcc tgttcctttt cccgagatac cagaattttt ccagcctcca aaagattggt
    26521 tcccaacaag agccccggtt gttgaacttt tatgtctatt tacatagaca actcctgatt
    26581 ctatattatt tacaaaatca tttatctctt ttttattttt cgaataaatt ccagctgtta
    26641 atccgtattc agaattatta cacagatcga gagcttcaga aaattggtgg aattcattaa
    26701 tacatagaaa cggcaagaac aactccttct ttaaaaggat attattagaa gatacttcat
    26761 ttactatcgt aggagaaacg taatatccat attttaaatt accttcttct ataacttttc
    26821 ctccatagag aattttgcct tcctgtgaag ctatatttac atatttctta aagttttgaa
    26881 aggcattaga gttaatcaat ggtcctagat cgcaattcct tttatcagaa tattctatat
    26941 ttattttttt aacatttaat attaatttct caataaactg attctttacc tttttatgaa
    27001 cataaaccct tgaacatgca ctgcattttt gtccgccata ggaaaaagca gctcgactta
    27061 ctccctctgc agctttgtcc aaatcagcgg tttccgtaac aatagtagga ttttttccgc
    27121 ctaattctgc aattattggt ctagatttta cattagcaga atttttataa agagtatatc
    27181 caacatcttt agaacctgta aacactatac cagcaatctt attgctgttg attattattc
    27241 ttccgatttt agcagcatcg ccagtaatat aatttaggac accaggcggt agacctgatt
    27301 ctatcattat ttcaacgatc ttgtatccta ttataggggt attacttgct ggttttgcaa
    27361 cggccgtatt tcctgtaata attgctccaa tattcattcc tacaaaaata gcagcaggaa
    27421 aattgaaagg cgatattatt gcccaaactc cataaggttt catgacacta atactttttt
    27481 gtgttcttgc tattgtttta tttttagtaa catatccatc attgttgatc atatcaatac
    27541 aataatagtt aataaaatct attgcttcat caatatctgc aattgattca tatctatttt
    27601 taccattttc aaatgttagt agtgcagcta atggaaattt attagatcgg atagtatttg
    27661 ctgctttttt aagaattcca atcctctttt gatattcaag tttactccat tctttaaatg
    27721 attggaatga tgactctata gctttattgc ctagttgatc tgatatcttg gaaacatatc
    27781 ctagtatgat gcgtcggtcg actggagaga catgtttata tttatctcta gtataaattt
    27841 tttcgttatt tataatgaca gggtaatctt gattattcaa ttctttcatt aaagattcaa
    27901 ctgcattatc aaatttatta tggaatatat tctcttgatt tcttttgata taattttcat
    27961 aagttagttc attcctaaat tgcatatgaa tattatagat ttaatattta ttaaacaatt
    28021 tattatcaat taccattatt attattgata tgactcacaa ttataaaaat aatcatgcta
    28081 atattatgat caagaaaaaa tctatagcaa tagtaaaaaa aataaaaaaa cttgcttata
    28141 attcaacatt tgtatatcct ttagtgatta agaatggtca aggatgttat cttgaagata
    28201 tagatggaaa acaatattta gattttactt ctaatattgc ctcctgtcca ttagggtata
    28261 atcatccaga acttaatgag attataacta gatatggagg tataggcgcg cacaaaatag
    28321 caggacagga tttttattgt gaagagcata gtcaactagc tgaaaaatta ctttcaatta
    28381 gccatcccaa ttcaaaagtt ttttttataa atagtggtgc agaagcagta gagaatgcac
    28441 tcaaacttgc ttatagaaga agtgggccat taccaggaat ttcttgcata aatgcatttc
    28501 atggacgaac cttaggtgca cttgctttta catttagcaa ggaagttcaa aaggcaaatt
    28561 ttcctgagtt cccagcaaag aggataaaat tttgtatttc agataatgat cctgaaatag
    28621 acacaattca aaaaatagtt agtgagtata aaatttcctt tattattaca gaactagttc
    28681 aaggagaagg aggagtaaat gtagcaagta aaaaatttgt aaacaatctc aataatacat
    28741 ctataaaata tgacatacct ctcattatag atgaagtaca aactggatta ggaagaactg
    28801 gaaaatggtg gacatatcaa cattatggta tcaaaccaga tatcgtcaca agtgcaaaag
    28861 cactacaagt tggagcggta gtttttgata aaaagtttga accccgcaga ccaggagttt
    28921 tatctagtac atggggaggt ggtagcagaa tagatatggc tattggaata aaaacaattg
    28981 atattatttc taaagggaaa ttgttagaca aagtaaataa atcgggggat tttttaaaaa
    29041 aatgcttaag tgaactaaaa aataagcacg atataataat ggatgttaga ggacttggat
    29101 taatgttggg agtggaattt cataaacaag agaagagaga caaaataata aacacactat
    29161 ttaagaagaa actattagtt attccctcgg gtcttaaaac tattcgaata ttacctcctc
    29221 ttgttattac gcctgatgaa atctctaaag gattgaatat ttttgaaaag gttttaaaag
    29281 agtttaatcg ttaaaacttt tttttctcag atttttctcg tgcaactcaa tagatctata
    29341 caatgcaacg atagatgcag catttagaat ttttccctta aaaagcattt ctttgacctg
    29401 atctattgtt aatgactcaa ttttgatgct ttccattaag ccaagatgtt gtttctgagt
    29461 ttttacaagg tcataagctg caaatatgta ccctttttgt tcgaacatag aataatcaag
    29521 agtatattct cctacaaata ggatcttatt ggcgctataa ccagtttctt ctttgaattc
    29581 tctaattgca gcatccttag gagattcacc atcttcgata tatcctgaag gaatttcaaa
    29641 ttgcaatgaa tcaactaaat gtctgtaact tttaatcatt aatattttat ctgcagaaag
    29701 gaatgggaca actgtagaat agttttttct ttttcctcta acgtatatct ttttaatgcc
    29761 atttatatcc agtaactctt catatagctg aatatattca tttaaataga catttcgttc
    29821 atcttcgaga actttccatc cattgggtgc attttttagc aattttatag atataagttt
    29881 ttatatctta attaatttat cctgtcgaat cgtattttaa ctagaaaata ttaattattg
    29941 gttgacatgg atttatggtt tcataatgaa aatttaaatg acatctcatc cacactatca
    30001 ttgttagtaa tctgaaataa taaattggaa aagaatcaat ttaattggtg atcaaattaa
    30061 atcgatccta gtatctagta agtaatccaa aatccccacc ttttttacta atatataaac
    30121 atttaattcc agtttaacct atttaatttt tcagtctcaa gtttgggaaa aagcaatgtt
    30181 tgaaattacg attgcagtta tcataaaaat taaaatagaa ataagtaaaa agaagatctt
    30241 tacagcagat taaattgaaa attttctatg attttggaaa gtataaattc tacactgatg
    30301 tgattaattt ttcattaaca atccaaagtt aaaccttcat aatagtgaaa tctgcatgtt
    30361 gtgataactc aaagatatta tctatgttta aattaaaacc tgacaataca taacattaca
    30421 tttttttaat tccaaaatct atttcgtata tccacaactt ctttgctatt cttaaaaata
    30481 aattttttat tgacaatata atttagaata gaagcaatag atattgcaaa tataataatc
    30541 gcaccgtaat caatactagt ctttccaaat ccgtacacta tcgatatttg tattattact
    30601 ccaaccgaat tgaaaaaaaa atattttaaa tattgtctta atagtatgtc atatttcatg
    30661 gttctgtctt gaaaa
//
LOCUS       DASVEV010000270         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_144366, whole genome shotgun sequence.
ACCESSION   DASVEV010000270 DASVEV010000000
VERSION     DASVEV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144366"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VF222_11880"
     CDS             <1..1517
                     /locus_tag="VF222_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7143165.1"
                     /translation="FKLNKQYYSFNKGNVFFLVMSTQDSYSENSEQFNFVKQELEKAS
                     KDPNIDWIVVYFHKPMYTSPSKHAGLTSLRDIYHPLFDQYNVDLVLSGHNHNYQRTFP
                     LTFNTDDSDRPILVNKNPNIYNEIGAPIFITSGTGGTGLHSLGAPQAEFNVKQFGKFG
                     HLDIDVRRDASNKLIGTFYDLAGNKLDEFTITKTLSSTSPPQSSSSTSTTPPTSSTQS
                     LTATSTTPTTIDKSDIDVFGIKKIYPTKPGGEEWFMNMDTMKADPRLEISGSANDVKK
                     NSDASWSPISGDKVRLSVYTSESKGKFDDKNMPTYNLKELAATGHWFKENDWKNIEMG
                     GYFKLNTADDLGLGYSFYTRSIDHSSTHGGCGGATPKFNIGFDGEIKAKKEMWHISLL
                     DSPGVNDEDLSPSIVGKWLGIKGIIYNLPGDKGVKQEFWIDKTNEGKWEKVYEFADQG
                     GFGERGNNGPEKCGGSWDQLYTWGSPKSVFTWDQSDVSFKFLSVREILPPAPMT"
     gene            complement(1686..2456)
                     /locus_tag="VF222_11885"
     CDS             complement(1686..2456)
                     /locus_tag="VF222_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7143166.1"
                     /translation="MKYFDAHIHLTDDHFSDYIHHILPSLRSLKIKACSVTVDLTTSK
                     KSLILFNNQKDIITQFIGIHPQFALKENIEEFKEFFTSNIKYIDGIGEIGLDPSYLLE
                     NETQYSSQKKMFQLLLELAEKYNKPISIHSRKSMDDILQVLPSYKIQSSLLHWFNGNK
                     KHLKQAMDMNIFVSYGPVLLYSPDKKVLLKETNIDKILVETDGPVKYSNCFNYLPSSS
                     ISHLLSVIKYVGDTLNLDFEDISNLLKSNSENFLGKII"
     gene            2635..>2914
                     /locus_tag="VF222_11890"
     CDS             2635..>2914
                     /locus_tag="VF222_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7143167.1"
                     /translation="MIIITILYYNIMYVYPVIEESPDISDKSSLQKLNVVASFYPWYE
                     FTKKIGGEKIQLSTAIPFGVEPHEWEPSPQKISELNKADIIIYNSKGFD"
BASE COUNT         1053 a          423 c          489 g          949 t
ORIGIN      
        1 atttcaaatt gaataagcaa tattattcat tcaataaagg aaatgtcttc ttcttagtaa
       61 tgtcaaccca agattcctat tctgaaaact ctgaacaatt taattttgtt aaacaagaac
      121 tagaaaaggc gtccaaggat ccgaatattg actggatagt ggtatatttc cacaaaccaa
      181 tgtatacttc cccctctaaa catgcaggtc ttacaagcct aagagacatt tatcacccat
      241 tattcgatca gtataatgta gatttagtat tatctggaca taatcataat tatcaaagga
      301 cgttcccact aactttcaat acagatgatt cggatagacc aatattagtt aataaaaatc
      361 caaatatcta taatgaaata ggagcaccaa tatttatcac atcaggtaca ggcggtacag
      421 gtctgcattc acttggtgcc ccccaagctg aatttaatgt taaacaattt gggaaattcg
      481 gtcatctcga tattgatgta aggagagatg cttctaacaa gttaatcgga acattttatg
      541 atcttgcagg aaataagtta gatgaattta caataactaa aacattatct tcaacttcac
      601 ctccacaatc atcatcatca acatcaacaa caccaccaac ttcatctaca caatccttaa
      661 cagctacaag taccacgcct acaactatag acaagtctga catagatgta tttgggatta
      721 agaaaattta tccgaccaaa cctggaggag aagaatggtt tatgaacatg gataccatga
      781 aagcagatcc gagattggaa atcagtggaa gtgcaaatga tgttaaaaaa aattctgacg
      841 caagttggtc tcccatctca ggtgataaag taagactctc ggtatatacc agtgagtcta
      901 aaggtaaatt tgatgataaa aatatgccaa catataattt gaaggaatta gctgccacag
      961 gtcattggtt taaagaaaat gattggaaga atatagaaat gggaggttac tttaaactga
     1021 atactgctga tgatctagga ctgggatata gtttctatac aagatctata gatcacagta
     1081 gcacacatgg aggatgcgga ggagcaacac ccaaatttaa cattggattt gatggagaaa
     1141 tcaaagcaaa aaaagagatg tggcatatta gcttattaga ttcgcctggt gtaaacgatg
     1201 aagatctatc tccttcgatt gttggtaaat ggcttggaat aaaaggaatt atctataatt
     1261 tgccaggtga caaaggagta aaacaagaat tttggattga taaaactaat gaaggtaaat
     1321 gggaaaaagt ctatgaattc gcagatcaag gaggatttgg agaaagaggt aacaatggac
     1381 cagaaaaatg tggcggtagt tgggatcaac tttatacatg gggaagtcct aagagtgtgt
     1441 ttacatggga tcaatccgat gtatcattca agtttttatc cgtaagagag attttacctc
     1501 cagcaccgat gacttgattt taaattagtt aaatggactc ctgagcataa agcattataa
     1561 aaatgaatat gacttagcaa aagataattt tgattcaaat tttaatattt aattcatatt
     1621 atatataatt ttagaaagta ggttatgata cgtagtattg tttattgcat gcatttccca
     1681 tttttttaaa taatctttcc taaaaaattt tctgaattgc ttttcaataa atttgatata
     1741 tcttcaaaat ctaaatttaa ggtatcgcct acgtatttaa ttacgcttag aagatgtgat
     1801 attgatgagg aaggcaaata attaaaacag tttgaatatt ttactggtcc atctgtctct
     1861 actaatattt tatcaatatt tgtctctttt aagagaactt ttttatctgg tgaatataat
     1921 agaacaggtc cataagatac aaaaatattc atatccatag cttgttttag atgcttttta
     1981 tttccattga accaatgtaa taaagaactt tgaattttat atgatggtag tacctgtagt
     2041 atatcatcca tggattttct agaatgtatt gaaataggtt tattatattt ttcagcaagt
     2101 tccaatagaa gttggaacat tttcttttgg ctggaatatt gtgtctcatt ttcaagaagg
     2161 tatgagggat ccaatccaat ttctccaatt ccatctatat attttatatt agaggtaaaa
     2221 aattctttga actcttctat attctcttta agtgcaaatt ggggatgaat tccaatgaat
     2281 tgagttatta tatccttttg attattaaat aagattaaac tttttttaga agttgtgaga
     2341 tccacagtta cagaacatgc cttaattttt aaagacctta acgaaggaag gatgtgatgt
     2401 atgtaatctg aaaagtggtc atcggttaaa tgtatatgtg catcgaaata tttcatataa
     2461 aaaataaaat ttaagataac ttaaataagt ttgtaattca tgacttaaat aatgaaatta
     2521 taaagtgtaa cacttgatat ttatacaatg gcattactta tgtagtatac ctaatagtga
     2581 aaaattaaaa tatataactg taaatttatc tttgatacta tgtactattt atttatgatt
     2641 atcattacta tactttatta caatattatg tatgtctatc cagtgattga agaatcgcct
     2701 gatatatcag ataaatcaag tttacagaaa ctgaatgtag tagcatcatt ttatccatgg
     2761 tacgaattta caaaaaagat aggaggggaa aaaattcaac tctctactgc cataccattt
     2821 ggagtagaac ctcacgagtg ggaaccctct cctcaaaaga tttcagaatt aaataaggct
     2881 gacattatca tatataatag taaaggattc gact
//
LOCUS       DASVEV010000271         6216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_921425, whole genome shotgun sequence.
ACCESSION   DASVEV010000271 DASVEV010000000
VERSION     DASVEV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921425"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..1344
                     /locus_tag="VF222_11895"
     CDS             565..1344
                     /locus_tag="VF222_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143168.1"
                     /translation="MTGILSLFAVGMILSSPLTASFAAPQQQPNDYLDLEKTIVKIKQ
                     DPMTETNSIADIIYKIGGFLPEDEMVAPFGYGVVTAVTNETGGTELNVIATTSHAGLL
                     DADAQEGDPNNPILHNHYAVLGSNANCGDRPSIDALSGEELGQVFVNGKTIIVKDLPS
                     SAPDSTEQIEITPGTNIQFVASFLLEVEGPSTSPVVCVSLVEVQNDRAVIFGEQEEQK
                     PDYPRPDYGNDYGYENNYHGEDEYGYENHYSMENSYDMEYN"
     gene            complement(1601..2764)
                     /locus_tag="VF222_11900"
     CDS             complement(1601..2764)
                     /locus_tag="VF222_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HEX7143169.1"
                     /translation="MTEIKNCIIRKTYTTLDSFDSNLDIVNMENGLYNMRTGEFTQHT
                     PDYYSLNQKPFPYNPKARPKRFIKFLKEVLYSEDLLTAIDIIALTFLRYNPHELYIIL
                     IGVGANGKSVYTGLITNLHGLKNVSNIPLKSLLNNRFALARLENKDVNIDTELSSATI
                     NDMSILKRLTGKQPIMIEKKGIDPYDAYLHAKQIFNANHMPINSDNSDARYRRETPLT
                     FPNQFEGIKDDPDLLNKLSTKEELSGIFNIIAHVLKNRIIKTQRVHINEKTINERREK
                     AELIHDPLNSFIKKGIAKDSVDSDSETKDDFHLAYTRLCKFHKLPIQQKETLGSILKK
                     KPYEWKDGKKTISKENRKTVWKGIRLKDKWKNDGVFLQQTIDGKYKDEEDESI"
     gene            complement(3450..3596)
                     /locus_tag="VF222_11905"
     CDS             complement(3450..3596)
                     /locus_tag="VF222_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143170.1"
                     /translation="MAAGLSYEQDWIQHSPEQEQDFVIASYETSYHEKTFKVLGKSKE
                     SWGL"
     gene            complement(4261..4383)
                     /locus_tag="VF222_11910"
     CDS             complement(4261..4383)
                     /locus_tag="VF222_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143171.1"
                     /translation="MAKQIADNHVKNMVICTGRVDTKMIKDILLKRDTIPPIEI"
     gene            complement(4376..4555)
                     /locus_tag="VF222_11915"
     CDS             complement(4376..4555)
                     /locus_tag="VF222_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7143172.1"
                     /translation="MDTNLKSVFLFTKEVLPYMSENKPRVIINVSSGAGMYGFSNLSS
                     YGESKFGIIGLNESG"
     gene            5536..6063
                     /locus_tag="VF222_11920"
     CDS             5536..6063
                     /locus_tag="VF222_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7143173.1"
                     /translation="MRKKLINKRNKKKIIQKKIIVIGISLTVIGLIVYGGINSLMSSN
                     GNFLLSPPRNLFMKATYLPTEGYAYTSQSTGTAKLLNSGVGSRSGLGHNPTITLNQGN
                     TVSIHLINEDSEKHSKHNINIDEFDTHTKDLGYFQSQTVTFTANKTGTFNYYCSIHPE
                     MTGKIIVKSHAEAYK"
BASE COUNT         2175 a          891 c          833 g         2317 t
ORIGIN      
        1 tactgttata attccatatc cccaatcaat tcctggattg tgtttaggaa tatctccttg
       61 tgctttgaaa attatatctg ttattacttt atcatttatg tctattagtg tttgattaaa
      121 ctaccattaa ttgtaacata atttgataat aacataaaga agaagaaaca gtagtagaaa
      181 gaatagaagt agagaattga attgaaaata gaggttagtt aatgatttgc tattatcatc
      241 tccaattgta ggatcgttaa atattatatt gaatttatat aacaattgtc gattatttga
      301 cccaactaaa attagttatt cttatgtcta atcattataa ctgcaattca aaatttctca
      361 aagcaaaatt tttaactcgt ctaatcaata taagatctaa ttgttttaaa gatataaaat
      421 atgtaatact tttactattc attatcttta acattaattt tatttatatt ataaaatgat
      481 ataatataat atgaattgtg taatattcta catcacatat aaaaactaaa taagactgta
      541 gtacacatgg cgaaattaac tataatgact ggaatattat ctctttttgc agttggaatg
      601 attttatctt caccactgac tgcatctttt gctgcaccac aacaacaacc aaacgattat
      661 ctagacctag aaaaaacaat agtaaaaatt aaacaagatc caatgacaga aaccaattct
      721 atagcagata tcatttataa aataggtgga tttcttccag aagatgaaat ggttgcacct
      781 tttggatatg gagttgttac tgcagtaacg aatgaaacag gtggcacaga attaaatgtt
      841 attgcaacta cttcacatgc tggtctttta gatgctgatg cacaagaagg agaccctaac
      901 aatcccattc tacataatca ctatgcggtc ttaggtagta atgctaattg tggagatcga
      961 ccttctatag atgctctaag tggagaagaa ttaggacaag tatttgtcaa tggaaaaaca
     1021 ataatagtaa aagatttacc atcttcagct cctgatagta cagaacaaat tgaaattact
     1081 cctggaacta acatacagtt tgtagcatca tttctattag aagtcgaagg cccatcaact
     1141 agccctgtag tgtgtgttag tctagtagaa gtacagaatg acagagcagt aatctttgga
     1201 gaacaagaag aacagaaacc agactatcca agacctgatt atggaaatga ttacggatat
     1261 gaaaataact atcatggtga agatgaatat ggatatgaaa atcattactc aatggaaaat
     1321 tcctatgata tggaatacaa ttaactaaca acctagatag tgatagacag ataacaagtt
     1381 aagcaagaac agctctaaat agataattcc attctttttt ttgtttataa caataaataa
     1441 tcagttgtaa ttgaattttg aataataaaa gtgtaagagt aataaaatat taaattcaaa
     1501 gaaaagagaa gacataagtg gggtgtaaat aaaaaaaata ttttttttat ttgatttaag
     1561 cctggacacc ctggacagcc atgacacttt ttttatgaag ctaaatactt tcatcttctt
     1621 catctttata ttttccatca atcgtttgtt gtaaaaagac accatcattc ttccatttgt
     1681 ccttgagtcg tattcctttc caaactgttt tcctgttttc cttgcttatt gttttcttcc
     1741 catctttcca ttcatagggt tttttcttta atatactgcc aagggtttct ttctgttgta
     1801 ttggtaattt atgaaatttg caaagtcttg tataagcgag gtgaaaatca tcttttgttt
     1861 cgctgtctga atctaccgaa tcttttgcaa ttcctttttt aataaatgag ttcagaggat
     1921 catggattaa ttctgctttc tctcgtcttt cgtttattgt tttctcgttt atatgtactc
     1981 tttgagtttt gattattcta tttttcaata catgggcgat tatattaaaa attccagata
     2041 attcttcttt agtagataat ttatttagta gatccggatc atcttttata ccttcaaatt
     2101 gattggggaa agtaagagga gtttctctgc gatatcgagc atcggagtta tctgagttga
     2161 ttggcatatg gttagcatta aagatctgtt tggcatgtaa atatgcatca taaggatcta
     2221 tccctttttt ttctatcatt attggctgtt tcccagtcaa tctttttaaa attgacatat
     2281 cgttgattgt agcacttgat aattctgtgt ctatatttac atccttgttt tccaagcgtg
     2341 ctaatgcaaa tctattgttt aatagtgatt ttaatgggat attactgaca ttttttaaac
     2401 cgtgtagatt tgtaattaat ccagtataaa cacttttacc atttgcacct acaccaatca
     2461 aaataatata tagctcatga ggattatatc ttaaaaaagt taaagctata atatcaattg
     2521 cagttaatag atcttcacta taaagtactt ccttaagaaa cttgataaat ctttttggtc
     2581 ttgcttttgg attatatgga aatggttttt gatttaatga ataatagtct ggagtatgtt
     2641 gagtgaattc accagttctc atattgtata gaccattttc catattaaca atatccagat
     2701 tcgaatcaaa actatctagt gttgtatagg ttttacggat gatacagttc ttaatttccg
     2761 ttatagagcc gtgttaagtt cgtagccata ttttttgtcc atatctgtaa aatctgcaaa
     2821 aaccttttct tacaagattt tatgtatcta ccaagaattt acaaccctcc tacaggattc
     2881 tactctagat cttcgattcc ataataatat attttggtaa ttataataat atcattttaa
     2941 ttataaacaa attgttgtgt acagaaatga attcaccatg agatttagtt aaaaaatcag
     3001 aaaaaaaaat taaaaagaag attataaaaa atattataaa gagtatataa aataaacgag
     3061 aaaagaaacg atgcaattca gtaaaattac aatactgcat cgatatttaa tttttttatt
     3121 ccttcccgat taaatatgat ctattgcaaa aaaattttta attagttcct cctaaatcca
     3181 aaagttgtgt ttatataaat actatcaaaa tacaaacaat agaataatca tatgttcaat
     3241 tataaatact agtttaaact ctcattaagt ccaaatgatt ctgaaaccat ttagataaag
     3301 ataaaatagt tttaaatttt gtagttattc aagatacaaa aaactgaaat atagaaagtt
     3361 atttcttccg attgactatt aattcattat gctgtatgga tgaatgcgca atatctaatc
     3421 catgtgcatt ctttaaatgc tattgaattt tataaccccc atgattcttt ggatttacct
     3481 aatactttga atgtcttttc atggtaactg gtttcataac tagcaattac aaaatcttgt
     3541 tcttgttctg gcgaatgttg aatccaatct tgttcatatg ataatccagc tgccataaaa
     3601 actgcatcgt attcattatt attatcaaat tttcctcatt acattttttc attaatcgtt
     3661 ttacccagac aatcatttaa tatttaatta attatttaat tttctttttt ttccaatatc
     3721 caattattat aataattact ccaataataa ccccaaaaat tgaaaaaaca tacgagttag
     3781 ttattataaa acaatttgtt tgtagatcta caattgtatt attttgaggg tcgatctcaa
     3841 gttgtgaatt ataatattct gataattttg ttaaacagtt aaaactttgg aaatttaaat
     3901 atatacttaa tattgcactt attattaata taaacgaacc actagtataa tatctattcc
     3961 ttctagaggt aatcaagtaa aataataatt ttaatgattt cattttggtg agtgaaatta
     4021 tttaaatttc tctcatatat aaaattcatt agaagtagaa atgattctca ctcatggggg
     4081 ccggttatgt attagggttt catatataaa gaatctagca tgcaaagaaa tgtaccacta
     4141 tagaatcatt ttctaaatac tctatcattc atttattata aactagatta tttgtccatt
     4201 atggtaacat aattgattaa atatcacatt atatattttt tgcaacttat tcagggttaa
     4261 tcaaatttct attggaggga ttgtatctct ttttaacaat atatctttta tcatttttgt
     4321 atctactcta ccagtacata ttaccatatt ttttacatga ttatctgcaa tttgtttagc
     4381 cactttcatt caaacctatt ataccaaatt tactttcccc atatgaagat aagtttgaaa
     4441 aaccatacat tccagctcct gaactaacat taataataac tctaggttta ttttcactca
     4501 tatatggaag cacttctttg gtaaatagaa aaacactttt taaattagta tcaatagttt
     4561 atcccattct tcagttgtat tcattatact ataataaatt atacctgcat tattaacaag
     4621 aacattataa ctctcaaagg tattttagat aaagacaaat tctatcttcc aaagaagtaa
     4681 gttgaccgtt tcgacaagaa tgttgtctac tttagagtaa ctgatgaaga atctaaacgt
     4741 acaaagaata tgatacagat ttgatataat tttttattat ttaattgttg ataatcaatt
     4801 tctttgttca gccttcgttt ctggtagagg taaaggaaac aagttgttga acctttgttt
     4861 tacttctttt cctctgggcc tttagatact tcatactaaa aatctaacat tatactgtaa
     4921 tcattattat tgataggaga ttgacttccg tgtgtttttc cattacaatc tcttataagg
     4981 tctaaaagat attcatacct attttatttc agtaggccta gaatatctta aggagtagtc
     5041 ttccatcttt ggttttgaat ttgaatatgt tcaatatcct tttttataat aaataggagt
     5101 atttggcctt gctgttgtag ttgaggttat tttcttatta ttccctgcta tgttagaaca
     5161 tatatgtttc ttttttttga atatctccct atttattaga agctacattt taataaattt
     5221 gagtattaca ataatatgaa catggttttg ggtttgatac tgttagcata cagttagcat
     5281 ccatttcttc tctttatttt tctaatttat taatatctgt taaaatcctt cctaatttaa
     5341 ccaacattat ttacatatta ggccgtcatt tttagttgga tattatagat ataatcccta
     5401 taacaactat tatgatagat acagttagtt tccaaaaaaa tcaaccagct atgaatgtca
     5461 gagagatcct ttaacttata tatatgttaa ctttgttaac tactctttaa atacaaaaaa
     5521 ttttacaaat tttatatgag aaaaaaatta attaataaaa gaaacaagaa aaaaattatt
     5581 caaaaaaaaa ttatagtaat tggaatatca cttactgtta ttggtttaat tgtttatggt
     5641 ggtatcaatt ctttgatgtc ttcaaatgga aactttcttt tatctccacc tagaaatttg
     5701 ttcatgaagg caacatattt gccaactgaa ggatatgcat atactagtca atctacaggt
     5761 actgctaaat tattaaatag tggagtagga agtagaagcg gattaggaca taatccaaca
     5821 attacactta atcaaggcaa tacagtatca attcatctta ttaatgaaga ttcagaaaaa
     5881 cattctaaac ataatataaa tatagatgaa tttgatactc atacaaaaga tttaggatat
     5941 tttcaaagtc aaacagttac atttacagca aacaagacag gaacttttaa ttattattgt
     6001 agtattcatc cggagatgac tggtaaaata atagtcaaaa gtcatgcaga ggcttataaa
     6061 taatgaatat taacaatgat aatacaatta tgaatgatga aagaaacaac atactcacta
     6121 ttatagatag ttttattgaa cagataacca aaattagaag gttctttata ggggtttcaa
     6181 tttcatcaat tattcttgct cccattgcta tttcac
//
LOCUS       DASVEV010000272         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_922587, whole genome shotgun sequence.
ACCESSION   DASVEV010000272 DASVEV010000000
VERSION     DASVEV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922587"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            746..1375
                     /locus_tag="VF222_11925"
     CDS             746..1375
                     /locus_tag="VF222_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143174.1"
                     /translation="MNKRIFVYSIAISAVIALTLGLMQSSSIAYGQTAETTDTATATA
                     STTGGQNTFSAKGSINSIVIKDGAQPQQLNEQTSYVLDGKWKLEVINGNVTDFSSKFT
                     MVHLDGTNRHYMILMNFKQDNATAGTVQVQPDGTTTIKGILDVIEKSDFLNPEFDDVN
                     TQITINKYNTVSINVDDAQTNQHFAGQPIRGVVDEFIFGFKHDKEHDRE"
     gene            1711..1971
                     /locus_tag="VF222_11930"
     CDS             1711..1971
                     /locus_tag="VF222_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7143175.1"
                     /translation="MKYRGRTEIVATILEAAYGGVTKTKIMYVAFLSYGQLKEYLSIL
                     IENNLIEYLDGTQTFKTTEKGLNFLKMHNEIGELLHITAKND"
     gene            2051..>2238
                     /locus_tag="VF222_11935"
     CDS             2051..>2238
                     /locus_tag="VF222_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143176.1"
                     /translation="MVCKGICSRHRAIKPKSGQRYTIGQKRCQVCQIFINWQGFYCPC
                     CGYKLRGKPRNSKFKEMM"
BASE COUNT          908 a          270 c          332 g          728 t
ORIGIN      
        1 gagtaatttt taaaagaata tagttgttat tttcaaaaag atttgtataa cttttaagag
       61 tagaaccagt aaccaagatt atactaagaa gactgattaa acttaataat aagaataaat
      121 ttggtatttt caaaattaat tattgaattt atatcataat tatctaatta aacattttca
      181 aaaatagtga aaaaagatga agaaatattg aaaaaataat ttgataattt tattcaatat
      241 caaaagttta attattattg accacaagaa gtaagatatt acaaataatt caacaatatg
      301 aagttaataa tatcattgat ttgattttct gtaaatttga aatttatgga tatgaaaact
      361 ttagaaaata atttttacaa atttgacttc tttgataatt aaaataagtt tctaacaata
      421 aaagctaaat acaagacaat agtattatct ctacattgga ctcctctttt agaaccgcta
      481 tcggtattac tatagaattg cacttattgt ggtattggtg aaagagcatc atatacttgg
      541 tttgttctga aatatcttca tggttatcct aatgtaaaga cttacgatgg ttcatggtta
      601 gaagggggta atattatggg aaatcttatt gaaaaatgaa ttaattaatg aaaaaattat
      661 ttctttgtat atactgggat tcttgatgaa taatttcgat aaatgtccta caattctttt
      721 ataaaatagt actaggtgaa agttaatgaa taaaagaata tttgtatatt ctatagcgat
      781 atccgcagta attgcactta ctttgggatt aatgcaatca tcatctattg catatggaca
      841 gacagcagaa acaacagata cagcgacagc tacggcatcc acaacaggtg gtcaaaatac
      901 cttttctgca aaaggttcaa taaattctat agtaattaaa gatggtgcac aaccacaaca
      961 actaaatgaa caaacaagtt atgttcttga tggcaaatgg aagttagaag taattaatgg
     1021 aaatgttaca gatttcagct caaagtttac aatggttcat ttagatggaa ctaatcgtca
     1081 ttatatgatt cttatgaatt ttaagcaaga taatgcaaca gcaggtacgg ttcaagttca
     1141 accagatggt acaactacta taaaaggaat attggatgta attgaaaaga gtgatttctt
     1201 aaatccagaa tttgatgatg taaatacaca aataacaata aacaaataca atacagtcag
     1261 cataaacgta gatgatgcac agacaaatca acattttgct ggtcaaccaa taagaggagt
     1321 cgtagacgaa tttatctttg gatttaaaca tgataaagaa cacgacagag aataaataaa
     1381 tttttcaaac ttcacttttt tatttcttaa attataaaat tttttgatga ctatatctac
     1441 tagaagcgta actatataag aaaataatac atcttcttga aactgcgtat tattaaattc
     1501 agttcctttt aactcaacaa gaaagtaaaa attaagtttc taataataaa agctaactat
     1561 gacagcaaaa gaatcaaatt ttgtctaaat gcaaggtatt accttcctaa gttaacattt
     1621 acaaataata aatgtagaat attctacaat agctatctta tcttttcatg tcatattata
     1681 ctaatataat taaattactt aattaaaatt atgaaatata gagggagaac agaaatagta
     1741 gcaactattc tagaagcagc atatggaggg gtgactaaga caaaaattat gtatgtagct
     1801 tttctaagtt atgggcaatt aaaagaatac ctttctatat tgatagaaaa caatctaata
     1861 gaatatcttg atggaactca aacattcaag actacagaaa aaggattaaa ctttctgaaa
     1921 atgcataacg aaattggtga attattacat attactgcca aaaatgatta gttaattgga
     1981 taatataaat aataaatcct aatttttagt gtggatagat tcttaagact ataatcaatt
     2041 tagacaacta ttggtatgca aaggaatatg ttcacgccat agagcaatca aaccaaaaag
     2101 tggacaacgt tatacaatag gacagaaacg ttgtcaggtt tgtcaaattt ttattaattg
     2161 gcagggattt tattgcccat gttgtggata taaattaaga ggaaaaccaa gaaatagcaa
     2221 attcaaagaa atgatgat
//
LOCUS       DASVEV010000273         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1701431, whole genome shotgun sequence.
ACCESSION   DASVEV010000273 DASVEV010000000
VERSION     DASVEV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701431"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VF222_11940"
     CDS             complement(<1..713)
                     /locus_tag="VF222_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7143177.1"
                     /translation="MTKLGYWASQEQYSMKDLFKFVKEAEKSGFDQTLTSDHFHPWSH
                     NSGYGNFTWVWLAAAAERTKNMKFITGVTAAVYRYNPGIIAQAFASLDVLYPGRIGLG
                     IGTGESMNEVSVGFDWPSADTRLERTTESIQIIKKLWNKGSDGGGGKEIRLSNNNNNN
                     DNNNNKYDDDDSDGFVNFNGQYFKLKNAKLFTPPSTNISLYMAASGKEAIKTAGRLTD
                     GLITTSKPNNSDEAFDIFN"
     gene            complement(989..1459)
                     /locus_tag="VF222_11945"
     CDS             complement(989..1459)
                     /locus_tag="VF222_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7143178.1"
                     /translation="MIAIKSKRLLFIVIIILIIISFFIYALYKANYTQENKKHKDIEF
                     EGTVNHVVDGDTLDINDNRIRLSLVNTPERGQEGYMKAKKLVQNLCLNKKGEVDIDDG
                     QRRGDRYGRDIGVVYCDGININKALVENNLAIIYSKYCDISEFSNEEWAKPHCH"
     gene            2595..>2795
                     /locus_tag="VF222_11950"
     CDS             2595..>2795
                     /locus_tag="VF222_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143179.1"
                     /translation="MFNKSIVIVNDDRDLINVYSEALEMSGYDVSSFTDPSLAYQHIK
                     ENPNKYSLVITDDKMPDMNGLLL"
BASE COUNT          953 a          430 c          324 g         1088 t
ORIGIN      
        1 ttattaaata tatcaaatgc ctcatcagaa ttatttggtt ttgaagttgt aatcaatcca
       61 tccgttaatc tgccagcagt tttaatagct tcttttcccg aagctgccat ataaagagaa
      121 atatttgtag aaggaggtgt aaacaacttg gcatttttta gcttaaaata ttgaccatta
      181 aagtttacaa aaccatcact atcatcatca tcatatttat tattattatt atcattatta
      241 ttattattat ttgataatct tatttctttg ccaccaccgc catcacttcc tttattccat
      301 aatttcttta ttatttgaat agattcagtt gttcgttcta gtcttgtgtc tgcagaaggc
      361 cagtcaaaac ctacagaaac ttcattcatg gattctcctg ttcctatccc cagacctatt
      421 cgtcccggat ataaaacatc taaggatgca aatgcttgtg ctattatacc aggattatat
      481 ctgtatactg cagcagtgac tccagttatg aatttcatat tctttgttct ttcagcagca
      541 gccgctagcc aaacccatgt aaaattacca tagccactat tatgtgacca aggatgaaaa
      601 tgatcacttg taagagtttg atcaaatcca gacttttcgg cctcttttac aaatttaaac
      661 aaatctttca ttgaatattg ttcttgtgaa gcccaataac ctagttttgt catatagttt
      721 aatgtctgac attatattta attaatatat aaaagatgaa gttcaaatgt aataatatac
      781 tacaacaatt agaatcataa ttttcatttg aaacgaattg tctttacaac tcgtgaaaac
      841 aagtaataga tttaaatatt atatagtctt ttgaaagaag attgttatat actacgtatt
      901 tataagaagt attaaagaaa tataatatct aagctatatt aaatattgtt ttgaattttg
      961 tttcagattt tgtattatat attgtatttt aatggcaatg aggcttggcc cattcttcat
     1021 ttgaaaattc agatatatca caatatttag aatatattat tgccaaatta ttttctacta
     1081 atgctttatt aatattgatt ccatcacaat aaacaacacc aatatctctt ccatatctgt
     1141 cacctctgcg ttgaccatca tcaatatcaa cttcaccttt tttatttaga cataaatttt
     1201 ggactaattt cttggctttc atataccctt cttgtccacg ttctggagta tttaccaatg
     1261 ataatcttat tctattatca ttgatatcta aagtatcacc atctacaaca tgattaactg
     1321 tcccttcaaa ttctatatcc ttgtgttttt tattctcttg ggtataattt gctttgtata
     1381 atgcataaat aaaaaaggat atgataatta atataataat aacaataaaa agtaatcttt
     1441 tactttttat agcaatcata atcattttaa attaataact tgattatatt actatttgga
     1501 ataactttat ctctttaatc gtaaatattc atcgtatctt aatttatcta tttatttttg
     1561 attttattct cctcaactca actttcagct gatgggaatt ttccctatct caatactgat
     1621 atccaatact agtaatgata taataagaaa ttttaaataa tattataatc agacctagat
     1681 aaatatagat cagtagaaaa tgactatata gattctttgt gtttgttatt gaaagatgtt
     1741 aattcgaaat gacagattga tcgtctaaaa aatgactaat tatatacact atcaaatcaa
     1801 ataacatcta ctttatgtat tgttataata ccgttatcgt caaccatcat ctttaggtga
     1861 ggtaataaag gttctaactt tgatttttca tcaatacttc tattatcatt ggcataggta
     1921 tatgcatagt tatcccttct attgttaaag tagatcttct tttcccaaaa ccatcaatac
     1981 ctgtccatac tgtagcactt gaaactttct tttatatgat aatcaattgt tatatgttaa
     2041 aaataataac ttttgagaat gcctatctat ttataatgtt taatgctttt ttaattttat
     2101 aattgttatc gagaaaagaa attattgtta atactaatgc aagtatggct attaattatt
     2161 ggaaattgga tatttctgta tctttctttt tccatctaga atttaaatat ttaataaaca
     2221 tgagcatgat taattattat taaaatagat tcttctaata aactataact tcattatcac
     2281 gatagaaaat gactttacac taacagatac tataataatt taacaagtta atctatcata
     2341 gtcataatat agaatgagaa attgatggat aatcttacct ttgacaagtc tttcataatc
     2401 ttaagatctt ttctatgctt tcattctgtg tcattctatt caagactatg acagttagtc
     2461 atctcatggc ttctaatagt gaatctatta atcaattaat caataatggt caactaatcc
     2521 atcaatattt catattttta atcattagaa agaattgaaa cttaaatacg attttaatat
     2581 taaattattc attaatgttc aacaaatcta tcgtcatagt taatgatgac cgagatttaa
     2641 ttaatgtata tagtgaggct ttagaaatga gcggttatga tgtttctagt tttactgatc
     2701 cttcattggc ctatcaacac atcaaggaaa acccgaataa atattcatta gtaattactg
     2761 atgacaaaat gcctgacatg aatggcttat tactt
//
LOCUS       DASVEV010000274        13105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1118377, whole genome shotgun sequence.
ACCESSION   DASVEV010000274 DASVEV010000000
VERSION     DASVEV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118377"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VF222_11955"
     CDS             <1..1299
                     /locus_tag="VF222_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7143180.1"
                     /translation="DLGRPISQMKQDVLRNNYIDKISVALIGSCTNSSYEDMSRASGI
                     AEQADRKGAKTKTSLLVTPGSEMIRATIERDGQMKALKDIGGTVLANACGPCIGQWSR
                     PEIKMGEPNTIVTSYNRNFPGRNDGKRDTMNFIASPEIVIALALSGKLSFNPLTDIIE
                     SKDGTKFKLEPPQMAPEVPSNGFANVKDVYVEPSADPDNIDVIIDSNSDRLQKLEPFS
                     KWDGKDFMEIPILAKVKGKCTTDHISPAGPWLMYRGHLDRISDNLLLGAVNAFHDKQI
                     GNGKNLLNGKIESYPHVAREYKEKGLKWLIIGDTNYGEGSSREHAAMTPRYLGCVAVI
                     SKSFARIHETNLKKQGILALTFENSSDYDKIHEDDRISLIGLDKLEPSKPVKCKIIHK
                     EEEAKIREEEINLKHSYNHHQIEWFCYGSALNVLKSQTRQ"
     gene            1423..1920
                     /locus_tag="VF222_11960"
     CDS             1423..1920
                     /locus_tag="VF222_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX7143181.1"
                     /translation="MIKITKKYSFIVDAMLGKIAKKLRILGFDTIYISSSTNDSEILD
                     LLINTKRILLTSDKELFYRSKQYKCHSIIINKNTEIENLITIFSDLDIKFIDTLLTCH
                     RCSICNNKLEIIEYANLPKKEVYETVYKNNNEFYRCERCNKLYWHGSHIKNIILMIER
                     INKML"
     gene            2211..2801
                     /locus_tag="VF222_11965"
     CDS             2211..2801
                     /locus_tag="VF222_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HEX7143182.1"
                     /translation="MYKKSLDSSLFSNPINYVAKAIFFTKGKGVHKDYLTSFELALRD
                     AKISDLNLVTVSSIKPAQCKIISIQEGRKYLLPGQITFAVMARSSTNEPNRLIASSIG
                     LAYPADNDQYGYLSEHHSTGETALKAGDYAEDMAMEMLATIMGLPNDSSLTWNEKEEQ
                     WKLSGKIYKTQNFTQSAEGHKDGMWTTVLSTAVFIL"
     gene            complement(3147..4556)
                     /locus_tag="VF222_11970"
     CDS             complement(3147..4556)
                     /locus_tag="VF222_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7143183.1"
                     /translation="MNDLISDLQLLIRQPSVSAKNNGVVECSRLVKKIMEKAGIKTEL
                     LFPQEKNASYKNNNFNTVAAEIDNDHFLPPVVYGEVKSKRNPQGKTILFYNHYDVQPE
                     DPIELWENNEAFSGKVSGNYIYGRGSSDDKGELITRIKAVEYLLKSYGDVPCNIKFIV
                     EGEEEIGSPHLETFLIQNKEKLKCDGVIWEFGYIDEKERPIIMLGLKGILYVELIAKG
                     GPSRDVHSSLATLIENPSWILIDALKILRDINGKILIKNWYKEVREFSQEELRILDKE
                     PLDEEIFKREYGISKFLNNVNGIEAKKFYEGMPTCNISSLIAGYIGKGSKTIIPNKAI
                     AKLDFRLVPNMNPVIQFKRLCDHMRDHGFNKDLLEIKLLQKEWPARTTPVDHPFIKVV
                     EKSANAIFGSTIKSISSAATGPMYSFINILDIPCVCIGSSYKYGKSHSPNEFARLDLL
                     NKTTKWISRIIENFGKTKP"
     gene            complement(4699..5985)
                     /locus_tag="VF222_11975"
     CDS             complement(4699..5985)
                     /locus_tag="VF222_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143184.1"
                     /translation="MENDAMLIEKQAEELKFKARKDSARYDPLFKVGTEVEACLLDDK
                     GNSVNASPLIEELQNSKFFRKSGCMIDYEYGSGQFEFKTSPIPITNLLDLEVLYEEFI
                     IEYLEKAIEKVYKEKIVIPVFLGANPSPDILQDSIVTNNERYKKLFEWQCKFSDIEFE
                     GKKFKAAHIPAGIQGFHFHLQGRNPTYAVLMFNHILNLISSSIVLGANSKLFAGKVFS
                     FHEPRIYLYDQSEQQNSGFPAITTYLNNMEDYIDYIKSRKPIIARDYFDLEKERHDDV
                     RIRLNSEFYRVETRIISVQPTPKALMAMIEFFVGYIYRAILEESSGEKLLRSLSILRE
                     ERQASVQSGFHATSHFNMIDTIKLQLDYARKGLSDLNVKPEFLNILDSRVKNRISPSE
                     YVATLWDKKFNGSINQTIVEVISHIWERTKDNNPIM"
     gene            complement(6541..7080)
                     /locus_tag="VF222_11980"
     CDS             complement(6541..7080)
                     /locus_tag="VF222_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143185.1"
                     /translation="MSILTGKEILNRLNKDIIIDPFNLKNLNPNSYNLTLHDEIKFYD
                     CDFLDMKKDNPTKLIKIPNDGLWLNAGETYLGRTMEYTVTKNLVPIIKARSSIARLHI
                     HIINSAGFGDIGYSGHWTIPISVIKKTKIYPFVPICQIYYSDIIGEIDKEYNIDDNKY
                     QNSRDIMTSKIYKEFDSNM"
     gene            complement(7489..8628)
                     /locus_tag="VF222_11985"
     CDS             complement(7489..8628)
                     /locus_tag="VF222_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7143186.1"
                     /translation="MKLIHTKLLFDGINEKKNCFIGIEGNKIKFVGHNKPSGKHEIIE
                     NCEAVTPSFIDAHSHIGMVRAGEPSNEEEANEHMDSISPLSNALHSIYMDDPSFQESI
                     EAGVLYSVVLPGSGNIIGGKAVLIRNFSSNITNAFISEIGIKTALGYNPRSTIKWKGT
                     RPSTRMGAIAVLRDNLLKAKKAMSLLEKKKKLIDEIDPHLELFLDILKKKYKLMVHVH
                     KEDDVMIMIELIKEFKIEAIANHLCDVHNENIFQLLRLNKIPIIYGPLDSFPYKVELK
                     HESWRNIEKLLRSNSKFALMTDHPVLLQRNLFYCLRHLLRFGTSKAEAISKITKEPAD
                     IIGASTIGQVKPGFKASIILWNNDPFLLSSYAKTVFAEGEIVYRE"
     gene            complement(8928..11966)
                     /locus_tag="VF222_11990"
     CDS             complement(8928..11966)
                     /locus_tag="VF222_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX7143187.1"
                     /translation="MGELNKIFLSSSLIFFFLLYITSYFPTPVYAHPIITDSNPKQFQ
                     SLENPPDRVTVYFSEPIVLQYSQISVIDSEGNKVDEGNPESYKGDSSTISVPLKRDIG
                     EGTFTINSKVLSAVDGHVVDGSVVFSIGGGKADFSQAKAESKDIVHLLSIENSLSRIP
                     GYVGQIVIVGAAFIFLWIQKPLSKFEWVKSFLQSNLYLIKRNLIKLIIVGNVLVLLSI
                     IATILIQASAIGGSVGDVLTTEFGKIIIVRLGLALVLLAFLLFSYKKFNNSKKASNDV
                     KYIIAIICLGLGILFTNSLISHAAALKSFGPVLIDYFHGIAASFWIGGLIFLTFVFVS
                     KIFLIKDEELKAKIISMVIPRFSIIIIPVLGSISITGPLLLWSIEGNLSTTIASLYGK
                     ILIIKLLLAATMILLGGYHQFITGKRISTLSIRQIGEKGHSKETITNEPKFLKRFIWS
                     LRIEAFIGLGLLFVVSLMTNMVLPSGELPSSNITNNTETISLENQVRNSDIYSTVVYT
                     ENQKINIHLEPLRLGENKIFVDFSDFNNNPIVNIINATIKLSQIERGIGPILIDMEKI
                     SNGRFNAVIPLSALGLWNVEIEGKTLEPNTPNTIASFEINVKPRLSDLQFNITEYKTP
                     QSSLLLDPVYQASTDSIWVGDSLPGSGRLWEFGIENKNYQEHTINGINLITLSVFDPK
                     NNNILWFIDPTSNILGKFNTDTNKTETFELPNKGVISGLTIDNSASIWMSIIQDNSIL
                     RFDINKNNFETFRIPTEDSRPIGLIYDENNNYIWFAESIGKIGRLDVQTKTITEFPNV
                     TESNSVENDILGEPTFLLLDPQTSNIYISDHEKNSVLLFNPLTSEFKEYKLTDPNGLA
                     FGMALDAYDNLWIAQHVSDTLAILDPDTGETTNIDIPTSNSFVQYLVTDSKKDVWFAE
                     QRGNALGKVTIKFIPSNSPKMASTTSSITDKENNNNNQTDDKIANKKIFNALTFNDLF
                     GPFIIIAIAVSTILYVKSREQLRKKLLEIDNIEERKKTKG"
     gene            12149..12736
                     /locus_tag="VF222_11995"
     CDS             12149..12736
                     /locus_tag="VF222_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143188.1"
                     /translation="MNQFHTLICVVFLIGILFFLSLIPTCHNKVYAHIFSEIPNAIFK
                     EKDGYSIAFLPYPAHPKVNDNTTLLNFNVQKKGNDVTNTFISLIIMDKNSKKIVYQVP
                     YKFHEFADMTYSYTFQNKSKYSLSLLTKVAGDPKYENNPLIVNFDIEISNGNNQLLVT
                     VIFFVIFFFIMIFILVPKLAKKVLTFLKRIFKKME"
BASE COUNT         4554 a         1979 c         1829 g         4743 t
ORIGIN      
        1 gatttaggta gaccaatttc ccagatgaaa caagatgtat taagaaataa ctatatagat
       61 aagatttcag tggctctcat tggtagttgt acaaactcgt catatgagga tatgtcaaga
      121 gcatcaggta ttgcagaaca agcggatcgg aaaggtgcta aaaccaaaac ttctcttttg
      181 gttacaccag ggtcagagat gattagggca acaattgaaa gagacggaca aatgaaagcc
      241 ctcaaagata tcggtgggac tgttttagca aatgcttgtg gtccatgtat aggccagtgg
      301 agtagacctg aaatcaaaat gggggaacct aatactattg ttacatcata caatagaaat
      361 tttccaggta gaaatgacgg aaagagagat acaatgaatt tcatagccag tcctgagatt
      421 gtaattgcgt tggctcttag tggaaaactc tcattcaatc cacttacaga tataattgaa
      481 tcaaaagatg gaacaaaatt caaacttgaa cctccccaaa tggctcccga agttccatca
      541 aatgggtttg caaatgtaaa ggatgtgtat gtagaaccat cagccgatcc agataatatt
      601 gatgtgataa ttgatagcaa tagtgacaga ttacaaaagc tagaaccgtt ctcaaaatgg
      661 gatggaaaag attttatgga aatcccaatt cttgcaaaag ttaaaggaaa atgtaccact
      721 gatcatattt ctccagcagg tccatggcta atgtatagag gacaccttga tagaataagt
      781 gataatttgt tattaggtgc ggtaaatgca ttccatgata aacaaatagg aaatggaaag
      841 aatttattga atggaaagat agaatcatat cctcatgttg caagagagta caaagaaaaa
      901 ggtctgaaat ggctcataat aggagataca aactatgggg agggaagcag tagagaacac
      961 gcagcaatga ctcctagata tctaggatgt gtagcagtta tatccaagag ttttgccaga
     1021 atccacgaaa caaatctaaa aaaacagggt attttagcac tcacctttga aaatagttca
     1081 gattatgata agatacatga agatgataga ataagcctaa taggattaga taaattagaa
     1141 ccatctaaac ctgtaaagtg taagataatc cacaaggagg aagaagccaa aataagagaa
     1201 gaagaaataa atttgaaaca ttcctacaac catcatcaaa tagaatggtt ttgttatggc
     1261 tcagcactaa atgttcttaa atcccagact cgacaataat aattaataaa attttatatt
     1321 aaacttgcca gattcaatac tttactatta tttgaattta gtaatactat tatgaagtgt
     1381 ctgtcttctt tcccctaaaa ttagcatttt tattccatat aggtgataaa aattacaaag
     1441 aaatattcat ttatagttga tgcgatgtta ggaaaaatcg caaaaaaatt aaggatactg
     1501 ggatttgata ccatttatat ttcatcgagt acaaatgata gcgaaatatt agatctgctt
     1561 atcaatacaa aaagaatttt attaaccagt gacaaggaat tattttacag atcaaaacaa
     1621 tacaaatgtc attcaataat tattaataaa aatacagaaa ttgaaaattt aattaccata
     1681 tttagtgact tagacataaa atttatagat actctattaa catgtcaccg ttgttctatt
     1741 tgtaacaata agcttgaaat tatagaatat gccaacttac caaaaaaaga agtttatgag
     1801 acagtatata aaaataataa tgaattttat agatgtgaaa gatgtaataa attgtattgg
     1861 catggaagtc acattaagaa tattatttta atgatagaaa gaattaataa aatgctatga
     1921 agtttggtta gatattttag gtttttaaac cgatagtaaa tgaatgaaag ttagagtaat
     1981 taagatttag tatgatatca tccttaatac tatattctat taatttttaa aattaaattt
     2041 ataccacaac aaaattcaag taaaatctac tatataaata acaatagtta ttttaaagta
     2101 aataaaaata gaagagttca aaatctaaga aaagttatat ttttaaaaaa ttaatataat
     2161 tactttaaag aggaaaatct taataatcct tttttctact atctttgcta atgtataaaa
     2221 aatctctgga ttcaagttta ttctccaatc ctatcaatta tgttgcaaag gcaatttttt
     2281 ttacaaaagg taaaggagta cacaaggatt atttaacgag ttttgagctt gcattaagag
     2341 atgctaagat ttctgatcta aatttagtaa ccgtgtctag tatcaagcca gcacaatgta
     2401 aaatcattag tattcaggaa ggtaggaaat accttctacc tggtcaaatt acatttgcag
     2461 taatggctag atcatcaacc aatgaaccaa atagacttat tgcgtcttct ataggcttag
     2521 catatcctgc tgacaatgat caatacggat acctttcgga acaccactcc acaggagaga
     2581 ccgcactaaa ggcaggtgat tatgcagaag atatggctat ggaaatgctt gctacaataa
     2641 tgggtttacc aaatgattct tcgttaacat ggaatgaaaa agaagaacaa tggaaattat
     2701 ctggtaaaat ttacaaaact cagaatttca ctcaatcagc agaaggacat aaagatggaa
     2761 tgtggactac agttttgagt actgcagttt tcatcctcta gttagttttt aaattataaa
     2821 ttggagaaca taaaaataaa ttgaaataat attttgatat tttatatgaa atataatgta
     2881 agttaaaaaa tttagaacat tggaaatcct ggatacaaat ctatttaaag gaggatatcc
     2941 tgattagaga ggaataattt attttaattc tactttagat cataaatttg cctttctcca
     3001 taatacagta gcaatatatg aaagtgattc agatacaatt cgatcaattt gtttgtggga
     3061 ataaccttga catacttctg acatagtatc ataaaaatta aaaaaaagtt ttttcaatct
     3121 gataccaaca ataccttcat taatcattat ggttttgtct ttccaaaatt ttcaattatt
     3181 ctactaatcc acttggttgt tttatttaat aaatctaatc gagcaaattc atttggtgaa
     3241 tgagacttac catatttata tgatgatcct atacagacac atggtatatc taaaatatta
     3301 ataaaggaat acatcggccc agttgcggcg gatgagatac tctttattgt agaaccaaat
     3361 atagcattag cagatttttc aacaactttt atgaaaggat gatcaaccgg agttgttctt
     3421 gctggccatt ctttctgtaa taattttatt tcaagcaaat ctttgttaaa gccgtgatct
     3481 ctcatatggt cgcaaagccg tttaaactga atgactgggt tcatattagg tactaaacgg
     3541 aaatctaact ttgctatagc tttatttgga ataatagttt tagaaccttt tccaatatat
     3601 ccagctatta gactgctaat attacacgtt ggcattcctt cataaaactt tttggcctca
     3661 ataccattta cattatttaa aaatttacta ataccatatt ctctcttgaa tatttcctca
     3721 tctaaaggtt ccttatctaa tattctgagt tcttcctggg aaaattccct tacttcttta
     3781 taccaatttt ttattaaaat ttttccattg atatcacgaa gaatctttag ggcatctata
     3841 aggatccaag aaggattttc tattagtgtt gcaagactag agtgaacatc tcgtgatggt
     3901 cctcctttag ctataagctc tacatataaa atacctttaa ggcctaacat aataattggt
     3961 ctttcttttt catctatgta accaaattcc catattactc catcgcactt tagtttttct
     4021 ttattttgta taagaaatgt ttccaggtga gggctcccaa tttcctcttc accttccact
     4081 ataaatttta tattacatgg aacatcccca taacttttca aaagatattc aactgctttt
     4141 attcttgtaa ttaattctcc tttatcatct gatgaacctc ttccataaat ataattacca
     4201 ctaactttac cactgaaagc ttcgttattt tcccataatt caataggatc ttctggttgt
     4261 acatcataat gattataaaa caaaatggtt ttaccttggg ggtttctttt tgatttgact
     4321 tctccgtata ctacgggagg taaaaaatga tcgttatcaa tttcagcggc gactgtattg
     4381 aaattgttat ttttatagct agcatttttt tcttggggaa aaagtaattc cgtttttata
     4441 ccagcctttt ccattatttt ttttactaga cgagaacatt caaccacccc attattctta
     4501 gcagaaacac ttggttgcct tatcaagagt tgaagatctg atatgagatc attcatatgt
     4561 gaatctatta tagagttaat attattcaaa cttgttattc ttaaaattta taagaatata
     4621 tagttaaatt ttgaaatatc agtaaaatta aaaaagaaat aataaatagt attttattat
     4681 ataaacaaca ataccatact acattatagg attattatcc tttgttcttt cccatatgtg
     4741 agaaattact tcaactatag tttgattaat ggaaccatta aatttcttgt cccataatgt
     4801 ggcaacatat tcgcttggcg aaattctatt ttttactcta ctatccaata tatttaaaaa
     4861 ctcgggttta acatttaggt cagacaatcc ttttcttgca taatcaagtt ggagttttat
     4921 tgtatctatc atattaaaat gagatgtggc atgaaaacct gattgtactg atgcttgtct
     4981 ttcttctctc aagattgata gacttcttag taatttctca ccggaagatt cttctagaat
     5041 agccctgtat atatagccaa caaaaaattc aatcatagcc atcaatgctt ttggtgttgg
     5101 ctgtactgat attatccttg tttccacccg ataaaattcg gaattcaaac gtattcgtac
     5161 atcatcatgc ctttcttttt caaggtcaaa ataatctctg gcaattatag gctttcttga
     5221 tttaatataa tcgatataat cttccatatt atttagatat gttgtaattg caggaaatcc
     5281 tgaattttgc tgttcagatt gatcatatag atatattctt ggttcatgaa aggaaaatac
     5341 tttgcccgca aaaagtttac tgttagcacc gagtactatt gaagatgaaa taagatttaa
     5401 aatatggtta aacattaata ctgcataggt aggattacgt ccctgtaaat gaaaatgaaa
     5461 tccctgaatt cctgccggta tatgtgcagc cttaaacttt ttgccctcaa attctatatc
     5521 agagaattta cattgccatt cgaataattt cttatatctc tcattatttg ttactatact
     5581 atcttgaaga atatctggtg acgggtttgc tccaaggaag acgggaataa ctattttctc
     5641 cttgtaaact ttttcgatgg ctttttctaa atattcaatt ataaattctt catataatac
     5701 ttcaagatct aataaatttg taataggtat tggagaagtt ttaaattcaa attgacccga
     5761 tccatattca taatctatca tacaaccaga ttttcgaaaa aatttagaat tttgtaattc
     5821 ttcgattagt ggagaagcat tcacggagtt tcctttatca tctaacaaac atgcctcaac
     5881 ttcagtgccc accttgaata gtggatcata tctggcactg tcttttcgtg ctttgaactt
     5941 taattcttct gcctgctttt ctataagcat ggcatcattt tccataataa ataaaagtta
     6001 gacttccata tgaaatttaa ggtcaatgta tattaactcg aataatagga attattctca
     6061 ctttgtactt caaataagaa ggtattataa taacaattga ctaattatag caattaaatt
     6121 atataaatta tatgttattc tattaggata aaaatagtgg aatattttag ggtatatatt
     6181 gttttttatt gttaagaatg tataagttcc acataagaaa tatctatttt gtaatctgta
     6241 tgactctacc tattacatca aacagtaaca atatttaatt aatttattta tttgttttca
     6301 atattattta tagaatatta aattggatac gaactcgaaa actactggtc ttcgatttaa
     6361 tcatattaaa aattttctat agagataagt taattttagg taaagtaaca tatgcagata
     6421 ttaatttttg ttaagagaat taatacttat agaaatgtca aaataaaata acaattttca
     6481 aatttttctt tcacaaagat gcatagtttt tagtaaatct ctttggttga tagttatttt
     6541 ttacatattg gagtcaaatt ctttatatat ttttgaggtc attatatctc tactattttg
     6601 atacttatta tcgtcaatat tatattcctt atcaatttct ccaataatat cagaataata
     6661 aatttgacaa ataggaacaa aaggataaat ttttgtcttt tttattacag agattgggat
     6721 tgtccaatga ccagaataac ctatatcacc aaatcctgca ctattaatta tatgtatatg
     6781 aagtcttgct atagaagaac gcgctttgat aatgggtacc aaatttttag ttactgtata
     6841 ctccattgtt ctgcccaggt aggtttctcc agcattaagc cataacccgt catttggaat
     6901 ttttataagt tttgttggat tgtctttttt catatctaaa aaatcacaat cataaaattt
     6961 aatttcatca tgcagagtta aattataact atttggattt aaatttttta gattgaatgg
     7021 gtcgattatg atatctttat ttaaacgatt tagaatttct ttgccagtga ggatactcat
     7081 tgattcatta tctatataaa taacttaaaa ttcttctgaa taataataac tttataatag
     7141 gctaaattaa aatcaaaata gaaaatttag agttataata aaagagagaa agaaatagta
     7201 aaaaaacaat attaaaatac aaaataattc gtaaacacct ttttgacata gctatgataa
     7261 aaagtataat gcatactgat aatctgaagt taataaaaat acattttaca ataatcttat
     7321 ttactctaat gctttcccta gatcatcgga aatagatcaa tggaaatcat ttctactaat
     7381 tggcagatac accttttttt cctattccta aaccaaatat ccaatgataa aataaaaaca
     7441 atagttttat attttgttac tgtactattg aatattagaa aataattact attcgcggta
     7501 tacaatttct ccttctgcaa atactgtttt tgcatagcta gatagcaaaa aaggatcatt
     7561 gttccaaaga ataattgatg ctttaaagcc tggttttact tgtcctatag tggatgctcc
     7621 aattatatcg gcaggttctt ttgttatttt tgaaattgct tctgcttttg atgtaccaaa
     7681 ccttaataaa tgtctcaaac agtaaaagag atttctttgc aatagtactg gatgatcggt
     7741 cattaatgca aatttagaat ttgatctcaa taatttttct atatttctcc aactttcgtg
     7801 tttcaattct actttgtatg gaaatgaatc taaaggccca tagataatag gaattttatt
     7861 taaccgtaat agctggaaaa tgttttcatt atgaacatcg cataaatgat ttgcaatcgc
     7921 ctcgattttg aattctttaa tcaattctat catgatcata acatcatctt ctttgtgaac
     7981 atgtaccatc aatttgtatt tttttttcaa aatatctaag aaaagttcta aatgaggatc
     8041 aatttcgtca atcaattttt tctttttttc taaaagtgac attgcttttt tagctttcaa
     8101 gaggttgtca cgaagaactg ctattgcgcc cattcttgta gacgggcgtg ttcctttcca
     8161 ttttatagta cttctcggat tatatcctaa agccgttttt attcctattt ctgaaataaa
     8221 agcattagtt atatttgacg aaaaatttcg aattaaaact gctttaccac ctattatatt
     8281 tccacttcca ggtaacacta ctgaatacag aacaccggct tcaatagatt cttgaaacga
     8341 tggatcatcc atatatatac tatgcaaagc attagataaa ggagaaattg aatccatgtg
     8401 ttcatttgct tcttcttcat tcgaaggctc tcctgctcta accataccta tgtgactatg
     8461 agcgtcaata aatgaggggg tgaccgcttc acaattttct ataatttcgt gttttccaga
     8521 tggcttgtta tgaccgacaa atttaatttt atttccttca attccaataa aacaattctt
     8581 tttctcatta atgccatcaa ataataattt tgtgtgtatt aatttcataa gtgacttcta
     8641 tatttttctc tcagttttaa tatttataac attgatatta attattaacg atgtttatta
     8701 tagtaatatg tcactaaaat aaattttgtg taaataatta gatatctgat gaagacattt
     8761 attatgaatt tttctagatg ttacgatgta gtattttaaa agtaaatttt tatattgtta
     8821 actattcttt tatacctaca ttattacatt tttttcttcg ttttttatat agttagtcta
     8881 ttcactttat attttttaaa ttaaaataaa taaattattt tatcttgtta acctttagtt
     8941 ttttttctct cttcaatatt atctatctct aacagttttt ttctcaattg ttccctactc
     9001 tttacatata gtattgttga aacagctatt gcaattatga tgaatggtcc aaaaagatcg
     9061 ttaaatgtaa gtgcattaaa tattttttta ttggcaatct tatcatctgt ctgattatta
     9121 ttattattct ctttatcagt tattgaactt gttgttgatg ccatttttgg cgaattagat
     9181 ggaataaact taattgtaac ttttcctaat gcattacctc gctgttctgc aaaccataca
     9241 tcttttttgg aatctgtcac aagatattgt acaaaagaat ttgacgttgg aatatctata
     9301 tttgtagttt ctcctgtatc tgggtccagt atcgctagtg tgtctgaaac atgttgagct
     9361 atccataaat tatcataagc atctaaagcc attccaaatg ctaatccatt aggatcagtt
     9421 aatttatatt ctttaaactc agaagttaat ggattaaata ataatacaga attcttttca
     9481 tggtcgctaa tgtatatgtt agaagtttga ggatccaaca gaaggaaagt tggttcaccc
     9541 aaaatatcat tctctacaga atttgattct gtgacatttg gaaattcagt aattgtctta
     9601 gtttgtacat ctaaccttcc tattttgccg atggattccg caaaccagat gtaattatta
     9661 ttttcatcat aaatgagacc tattggtctt gaatcctctg tgggaattct aaaagtttca
     9721 aaattatttt tatttatatc aaatcttaga attgaattat cttggattat gctcatccat
     9781 atgctagcac tgttatctat tgtcaaaccc gatattactc ctttatttgg taactcgaat
     9841 gtttcagttt tattagtgtc agtattgaat ttacccaata tattagaggt tggatctata
     9901 aaccataaaa tgttattatt ttttggatca aaaacagata acgttattaa atttatacca
     9961 ttaattgtat gctcttggta gttcttattc tcaataccaa attcccacaa tcttccactt
    10021 ccaggtaggg aatcacctac ccaaatggaa tcagtactag cctgatatac aggatccagc
    10081 aaaaggctgc tttggggagt tttgtattcg gtaatattaa attgtaaatc agagagtcta
    10141 ggttttacat ttatttcaaa gctagcaata gtatttggag tattaggctc taatgtcttt
    10201 ccctcaatct ctacattcca taatcccaat gcactaagtg ggattactgc attgaatcgt
    10261 ccattagata ttttttccat atctattaga attggtccaa ttcctcgttc tatttgtgat
    10321 aattttatgg tagcattaat tatgttaact attggattat tattaaaatc agaaaaatct
    10381 acaaatattt tattctctcc caacctcaac ggttctagat gtatattgat tttctgattt
    10441 tcggtatata caacagtgga atatatatca ctatttctta cttgattttc aaggcttata
    10501 gtttcggtat tattggtaat gtttgaagat ggcagttcac ctgaaggtaa taccatgtta
    10561 gtcattaagg aaactacaaa caaaagaccg agacctataa atgcttcaat tcttaaactc
    10621 caaataaatc gttttaaaaa tttgggttca tttgtaattg tttctttgct atgacctttt
    10681 tcacctattt gtcttattga taaagttgag attctttttc cagttatgaa ttgatggtaa
    10741 cctcctaata gtatcatggt tgcagcaagt agtaatttga taatcaatat tttaccatac
    10801 agtgaagcaa ttgtcgtaga gagattacct tctattgacc acaataagag tggccctgta
    10861 attgatatac tacctaaaac tggtattatt ataattgaaa atcgaggaat taccatcgaa
    10921 ataatttttg cttttaattc ttcatctttt attaaaaata tttttgaaac aaaaacaaat
    10981 gtcagaaaaa ttaaaccacc aatccaaaat gaagccgcta ttccatggaa ataatctatt
    11041 aggacaggac caaaactctt taatgctgca gcatgagata tcaaactatt agtaaataat
    11101 atacctagtc ctagacatat tattgcaata atatatttaa catcattact tgcttttttt
    11161 gaattgttaa attttttata agaaaatagt aaaaatgcta ataacactaa agccaaacct
    11221 aacctaacta tgataatctt tccaaactca gtagttaata catctccaac tgatcctcct
    11281 atagcagaag cttgaattag tatagtggca atgatagaaa gaagtactag aacattgcca
    11341 acaattatta attttattag atttctttta attagataca aatttgattg aagaaaactt
    11401 ttgacccatt caaattttga taaaggtttt tgaatccaca aaaatatgaa tgcggctcca
    11461 actattacaa tctggcccac atatccaggt atcctactaa gagaattttc tatggataac
    11521 aaatgaacaa tgtctttgga ttcagccttt gcttgactaa aatctgcctt cccacctcca
    11581 atactaaaaa ctacagagcc atcaactaca tgtccatcaa ctgcagaaag tactttacta
    11641 ttaatagtaa aagttccttc acctatatct ctctttaatg gtacagaaat agtagaagaa
    11701 tcgcccttat agctttctgg atttccctca tctactttat tcccttccga atcaataacc
    11761 gaaatttggc tatactgtag aactataggt tcactaaaat aaactgtaac tcgatcaggg
    11821 ggattctcta aggattgaaa ttgcttagga ttagaatcag ttattatggg atgtgcataa
    11881 actggcgtag gaaaataact tgtaatatat aataagaaaa aaaaaattaa actagaagac
    11941 aagaaaattt tatttaattc tcccattgat atttctatta ttttatatta ccttcaagaa
    12001 ttaatttgtt ttctcgagaa ttttgccctc ttaaatgtta ttttgtaatt aataaaggta
    12061 taaagatcca ccttcagttt agctttttac atgttcattg tttaaaattt atttacaaat
    12121 atatatttga ttaagtaaaa tgatatcttt gaatcaattt cacactttaa tttgtgtagt
    12181 atttctaata ggtatactat tttttctttc tctaattcca acctgccata ataaggtata
    12241 tgctcatatt ttttccgaaa taccaaatgc tatctttaaa gaaaaagatg gttatagtat
    12301 tgcattcttg ccatatcctg ctcatccaaa agtaaatgat aatactactt tattgaattt
    12361 taatgtccaa aaaaaaggaa atgatgttac taatactttt atatcattaa taataatgga
    12421 taaaaactct aaaaaaatag tttaccaagt accatacaaa tttcacgaat ttgctgatat
    12481 gacatactca tatacttttc aaaacaagtc taaatattct ttatcattgt taactaaagt
    12541 tgcaggggat cctaaatacg aaaataatcc tcttatagta aattttgaca ttgaaatatc
    12601 aaatggtaat aatcaacttt tagttacagt catattcttt gtaattttct tttttattat
    12661 gatatttatt cttgttccca aacttgcaaa aaaggtacta acattcctca aaaggatttt
    12721 taaaaagatg gagtaaataa actcaacatt aaagatatta caaaattgga agagaattta
    12781 acttattttt attctttgtt aaataataaa tcatcaccag cggatgtgat gaaaattgtg
    12841 catttgacat ccaacctaac tgcaaaaagt gttttcctta aaacaataat ataataacaa
    12901 aagaatttta aatgagcgat agatattttt ttaattagat atattttaaa tgatttattc
    12961 gaatctttgt aattaaatct gtttacttat cttttagtcc aaagaagatt aatctattgt
    13021 ttaggaaaat tttatttatt atcagatcaa atttgttaat tattatatga ctacacattt
    13081 caatttcaat gaacaaattt actta
//
LOCUS       DASVEV010000275         4115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_924979, whole genome shotgun sequence.
ACCESSION   DASVEV010000275 DASVEV010000000
VERSION     DASVEV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924979"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF222_12000"
     CDS             <1..241
                     /locus_tag="VF222_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143189.1"
                     /translation="NYATKATGIKKVYAIIGGFHLSGEEYEDSIPLTITELLSIDPEY
                     IIPCHCTGWKASNEIIRAMPQKYLQTSVGSTFCFN"
     gene            579..806
                     /locus_tag="VF222_12005"
     CDS             579..806
                     /locus_tag="VF222_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143190.1"
                     /translation="MESTSIEKLQKLIDNNYKIEKIHPLIFGSDAEINIVSLTLVSKD
                     GKKENIRAYGEESHQLREFIRKNELFQDRGL"
     gene            complement(949..1626)
                     /locus_tag="VF222_12010"
     CDS             complement(949..1626)
                     /locus_tag="VF222_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7143191.1"
                     /translation="MINTYLFASSILFSSFLLLTVFLYSINFQQDNPLIIFDNSAKAF
                     FSDNSLNDYFREFMVYVTFYGREYIWGPVLIALFLLGGKVGRKTAIITGLSIIILVLV
                     GGEIKNIIDRDRPELANNIYLLEEEESKKSYPSGHALIVSAGATGVICLYRGNLRRKI
                     ISLLLVIEAVLVIFSRIYLGLHYPLDIVGGILLGVSVAFFFVGFQKQIDLLSKNIFSF
                     IKSRIVK"
     gene            1929..2228
                     /locus_tag="VF222_12015"
     CDS             1929..2228
                     /locus_tag="VF222_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143192.1"
                     /translation="MTTLQKDIHEITSILCKTFEVSGIERNYDDSFGKIGKILYRYGI
                     NYNPKELRIVFTLCMQEFFESIPQEQWKLNDPSFVKQSERYAINKFKNWVVDQVT"
     gene            2513..2773
                     /locus_tag="VF222_12020"
     CDS             2513..2773
                     /locus_tag="VF222_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143193.1"
                     /translation="MSQINGSSKDNSQGDQENGNSQNSNWVDLIGALFDRLTGKEAVI
                     TYEFENLIVDIPRASGPGGKELGSAKWTINGKLIIKAEAVQK"
     gene            2797..3456
                     /locus_tag="VF222_12025"
     CDS             2797..3456
                     /locus_tag="VF222_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143194.1"
                     /translation="MTDWDNIIKSKKNVRTKDNISVGNVVANYDNYILVIDGVINKHT
                     YKIPKSSIESYNGSEILLNINHQDLKTFSEDFHKTNHSNSIESMFMDILVKGLSFMSG
                     TVKIAIQDNESLRIESSDKKIKLHIIDPSLFEIPIETIKDNKMKDFLKYIKEAKEFAH
                     KLTENGLTLFILNKDENAITLGKDAHPSFSKLITRSDDIQIDSIRKSLKLATDIKDED
                     K"
     gene            3825..>4115
                     /locus_tag="VF222_12030"
     CDS             3825..>4115
                     /locus_tag="VF222_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7143195.1"
                     /translation="MKFCLEVWGTNYETIKETCRFAEKSGYDGFFYGESLTDIDLDCW
                     TVLSSLINITSKIKLGPVITYLFPEYRNITLLAKQAITLQNLSKGRLEFRTGA"
BASE COUNT         1671 a          511 c          583 g         1350 t
ORIGIN      
        1 aaattatgct acaaaagcta caggaataaa aaaagtttat gcaattattg gaggatttca
       61 cttatccgga gaagaatatg aagattctat ccctttgaca attactgaac ttttgagcat
      121 tgatccagaa tatataattc cctgccactg cactggttgg aaagcttcta atgaaataat
      181 cagagcaatg ccacaaaaat acttacagac aagtgtagga tctacttttt gttttaatta
      241 atattttaat ataagcaagc tttagtttgg aaaaaagtta aatgcaattt atactgtaga
      301 taggtgataa ttttattcta atatattaaa cttactaagt gattatttgg tatctgttta
      361 atttaaattg tattagctcg ttttgtaaaa tattaatagt taatgattca ggtatgcaca
      421 tatgaaatca gatttgtgac cacataattc aaattattaa gtaacagatt aacccagttt
      481 aaacataaaa tcaagataat tatacccaat aactaagata tcaattgctg tagaatagta
      541 tgctaatcca cttatagaat aaatttctta aaagaataat ggaatcaact tctattgaaa
      601 aactacaaaa gctaattgac aataattaca agattgaaaa gatacatcct ttaatttttg
      661 gatcagatgc tgaaattaat attgtcagtt taaccttagt ttcaaaagat ggaaaaaagg
      721 aaaatattcg agcatatggt gaagaatctc atcaactaag agaatttatt agaaaaaatg
      781 aactctttca agaccggggg ttataataat atcaaaaaaa taaaaccaag tacggctgcc
      841 ttgcaaaaaa tcaaacatta ccactatgag agaaactatt ttcataaaga gaatcttgaa
      901 ataatcaaat aaactgttaa cacatattca gtaactttct tcatcaattt attttactat
      961 tcttgatttt ataaaagaaa atatattttt tgataaaagg tcaatttgtt tttggaatcc
     1021 tacaaaaaag aaagctactg agacacctaa caaaatacca ccaactatat caaggggata
     1081 atgtaatccc agatatattc gtgaaaaaat tactaatact gcttctatga ctaacaataa
     1141 tgatattatt ttgcgcctta agttaccacg atataaacat attacccctg ttgcaccagc
     1201 tgaaactatt aatgcgtgac ctgatggata cgattttttc gattcttctt cttctaatag
     1261 atatatattg ttagctaact ctggcctatc tctatctatt atattcttaa tttctccacc
     1321 taccaatact aaaattatga tggataatcc tgttataata gcagtttttc ttcctacctt
     1381 acctcctaat aggaacagtg caattaaaac aggaccccag atatactctc taccataaaa
     1441 agttacatag accataaatt cacgaaaata atcattcaag gaattatctg aaaaaaatgc
     1501 tttagcagaa ttatcaaata ttattaaagg attatcttgc tgaaaattta ttgagtataa
     1561 gaatacagtt aagagtaaaa aagaactaaa aaggatagaa gatgcaaata agtaagtatt
     1621 aatcatctat aataaaccat gtaagaggtt tttaatattt taatttatca taaattttag
     1681 tgtttatagg attaataata atagagttta aaaagttaaa agtaatttaa attttttaaa
     1741 tatagaattt gatttaattg ttttgctata tctatttttt gctgaaatgc aattgtcatt
     1801 tataaattga aattttaggt ttaccaaggt catttgctgt aatatcttgg tatctaacaa
     1861 gtagtataat tatcaaaatc tctaataaca taaaataaaa taaatttgat attgtttaag
     1921 catatagtat gacaacatta cagaaggata ttcatgaaat aacatcaata ttgtgcaaga
     1981 cttttgaagt atcaggtatt gagagaaatt atgatgattc ctttggtaaa attggaaaaa
     2041 ttctttatcg ttatggaata aattacaacc caaaagaatt acgtattgta tttactttat
     2101 gtatgcagga attttttgag tctattcccc aagaacaatg gaaattaaat gatccttctt
     2161 ttgtaaagca gtctgaaaga tacgcaatta acaaatttaa aaattgggtt gtagaccagg
     2221 taacataaca aagtcactaa aatactaatt tatgccaata ttattattat tattattcta
     2281 caattagaat taagattgac ttagatcaaa aatactgtgg ttcttagttg atgatagtag
     2341 aattgaataa atattggtaa ttatcattat aaattaaata attagtaaca agggtaacaa
     2401 ttttataaat tttaaccata aaaagttaat tgaatatagc atatctgtta tgaataatat
     2461 aaaaaaatat catgaatgta atttttaaat aacatgttaa gtattagaaa atatgagcca
     2521 aataaacggt agtagtaaag ataattctca aggagatcaa gaaaatggta actctcaaaa
     2581 cagcaactgg gtagacttaa tcggtgcact ttttgatcga ttaacaggaa aagaggcagt
     2641 aataacatat gagtttgaaa atcttatagt agacataccc agagcttcag gtccgggagg
     2701 aaaagagtta ggatccgcta aatggactat aaatggaaaa ttaataataa aagctgaagc
     2761 agtacaaaag taaaacatac taaaggatga ttttcattga cggattggga taacataata
     2821 aaatcaaaaa aaaatgtgcg cacaaaggat aacatttcag taggaaatgt agttgctaat
     2881 tatgacaatt atatactagt aatagatgga gtaataaata aacatacata taaaatccca
     2941 aaatcatcta ttgaatctta caatggatct gaaatattat taaatattaa tcatcaagat
     3001 ttaaaaacct tcagcgaaga ttttcataaa actaatcatt caaactcaat tgaaagtatg
     3061 tttatggata ttcttgttaa aggattgagt tttatgagtg gtacagtcaa gatagctatt
     3121 caggataatg aatctttaag gatagaaagt tcagacaaaa aaataaaact acatataatt
     3181 gatccttctt tgtttgaaat tcctatagaa actataaaag ataacaagat gaaggatttt
     3241 ttgaagtata tcaaggaagc aaaagaattc gctcataaac ttacagaaaa tggattgaca
     3301 ttgtttatac ttaataaaga tgagaatgca attacattag gaaaagatgc ccatccttcc
     3361 ttctctaaat taattacaag aagtgacgat atccaaattg atagtataag aaagtcatta
     3421 aaacttgcaa ctgatattaa ggatgaagat aaataaaatt ttttgattat atagattaga
     3481 tagatagata gatagaaaga aagaaagaaa gaaagaaagg tgaataggca gttcaagtag
     3541 agcgtaataa catttaaaga taaaaataaa ttttattggg ctaattgttt atatgatacc
     3601 attattgtga tattattaat catttataaa tatttagtga acatctcctt tatatgacca
     3661 tccttacaaa atcttatatt ttttctaata gtaaataatc caactaaaat acatatacat
     3721 ataataaaat ttaattcctt tattttcatt tgaacaaata atcatagccc cttacaaaat
     3781 agtatttcag actttaataa caataaaata attataataa tataatgaaa ttttgtctag
     3841 aggtctgggg taccaattat gaaacgatta aagaaacttg cagatttgct gaaaaatcag
     3901 gatatgatgg atttttttat ggtgaatcac taacagatat cgatttggat tgttggacgg
     3961 tattatcgtc attgataaat attacaagta aaataaagct tggacctgtt ataacatacc
     4021 tttttccaga atatcgtaat attactctat tagccaagca ggcaataaca ttacaaaatt
     4081 taagcaaagg aagactagaa ttcagaacag gtgcc
//
LOCUS       DASVEV010000276        12390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_344931, whole genome shotgun sequence.
ACCESSION   DASVEV010000276 DASVEV010000000
VERSION     DASVEV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344931"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF222_12035"
     CDS             complement(<1..192)
                     /locus_tag="VF222_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016272 - prefoldin complex [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit"
                     /protein_id="HEX7143196.1"
                     /translation="MSQENELPPWLKEQISRLQQLQQNLQAIMMQKQQIELEIVETER
                     ALEELSKTTTSDSIYKAAGP"
     gene            complement(233..511)
                     /locus_tag="VF222_12040"
     CDS             complement(233..511)
                     /locus_tag="VF222_12040"
                     /inference="COORDINATES: protein motif:HMM:NF020899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HEX7143197.1"
                     /translation="MRNFKAKINISLDLSSPSETKKFLESLYNALEPDTKILVTPQIK
                     LHLSNKDCMSHLIIEISTSDMAELRSAINSYLRLIKTTHLCITNLLFI"
     gene            complement(492..731)
                     /locus_tag="VF222_12045"
     CDS             complement(492..731)
                     /locus_tag="VF222_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37"
                     /protein_id="HEX7143198.1"
                     /translation="MVNRNRKGQTVLKGLGTKFGATVRKRYSKVYRTLKQKRRCPSCG
                     SLKFVRVACGIWNCPKCNFKVAAGAYDISIEKLQS"
     gene            complement(732..1550)
                     /gene="rrp42"
                     /locus_tag="VF222_12050"
     CDS             complement(732..1550)
                     /gene="rrp42"
                     /locus_tag="VF222_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HEX7143199.1"
                     /translation="MSLSKKSPIVIEQLRKRQMFDLITNGKRLDGRNLEDIRSLEIDI
                     DIIKKADGSAKVKLGNSEVIAGVKVETGAPFEGLENKGALIVTAEVLPIASPYIEPGP
                     PDEETIELARVVDRGIRESQMIDLDKLVLIPGKTVYVIFVDCSIINADGNLFDATSYA
                     VVAALLSTKLPIFEIRDEKVVDTGKKQEPPIRTIPVSITSIRIGNNIIIDPTAEEEAC
                     MESRITITTNSNNEYAAIQKGSTGYFTLEQIKFAAQTARVKAETLRTKLKELIK"
     gene            complement(1547..2308)
                     /gene="rrp41"
                     /locus_tag="VF222_12055"
     CDS             complement(1547..2308)
                     /gene="rrp41"
                     /locus_tag="VF222_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HEX7143200.1"
                     /translation="MDQTILIDENGKRTDGRSIDDLRDVKITIGVLKNADGSASIEFG
                     KNKIIVAVYGPREVHPKHMALPDRCVLRCRYHMSPFSTDTRKNPAPSRREVEISKVMR
                     EALEPAIMLEDYPRAAIDVFVEVLQADGGSRCAGIAAASVALADAGVNMRDLVAACAA
                     GKVNDKIVLDINDIEDKEGTADMPVAYLPNLEQITLLQLDGILNESQFDECVEKAIHG
                     CKMVYEIQRKALIEKYFGNDLPEGEEKELEENKEN"
     gene            complement(2327..3151)
                     /gene="rrp4"
                     /locus_tag="VF222_12060"
     CDS             complement(2327..3151)
                     /gene="rrp4"
                     /locus_tag="VF222_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HEX7143201.1"
                     /translation="MLEIKKRYVVPGDVIIEGNYRPLMNVIKKNNFIIATRIGIAESS
                     KDGIKVIPLSGVYIPRINDIVIGKIVDNSSLSWEVDINACFAAHLPAQDVFGRDFSPA
                     KDDMKKRLAPGDLVTARIITFDRTRDPMLTIQEKDLGKISNGEFLKISSTRVPRLIGK
                     RGSMIQTIEQATQTRILIGQNGILVVTGKNGEGIALAFKAIKLVEEEAHTSNLTQKVK
                     DLLNVSEEITQTETNNSSVNNEDYVNNSNTDNNSSNMENDHSSNINTESPKEVQPK"
     gene            complement(3285..3974)
                     /locus_tag="VF222_12065"
     CDS             complement(3285..3974)
                     /locus_tag="VF222_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HEX7143202.1"
                     /translation="MTDNKFSIVRLILNADKFEILVKPDPALDYKLGKKIDISNILVS
                     DEIYSDANKGTRVPNEKLIKNFKTKDQIEIAKQILEHGDVNLTTDQRRRMVEEKKKQI
                     VQYINRNFIDPKTHLPHPPLRIENAMEQIRLIIDPFKRPEEQAKKIIDSLRKILPLKS
                     ENLQLIVTVPPQFSAQSYSIIKGIGYLKDEKWLQDGSLQVIIEINAGIRGTFIERINS
                     ITKGSAQIQEQ"
     gene            complement(4061..4513)
                     /gene="rpsI"
                     /locus_tag="VF222_12070"
     CDS             complement(4061..4513)
                     /gene="rpsI"
                     /locus_tag="VF222_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7143203.1"
                     /translation="MTKTTTIDLYPGQRKTSRAVATISKGNGKVRINNIPAEIIQPDI
                     SKELILTPVNIIGDIRDKVDITVKVEGGGFMGQAFASAVAISRALSGEAKGGRDPKDH
                     PLSKSVRQEIRKKITEFDRHLLAGDPRQAESKKFGGPGARRKKQKSYR"
     gene            complement(4520..4996)
                     /locus_tag="VF222_12075"
     CDS             complement(4520..4996)
                     /locus_tag="VF222_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7143204.1"
                     /translation="MDKSKNSNTVSNINKPIIVDASNCIAGRICSIVSKLLIQGNRIA
                     IINSERAMLSGNRYKTIEEYKKYLEVSSVTNPIHGPYHPRRPDRILTKMVRGMVSRRK
                     PSGILAFKRLRVYIGMPTEFQSSKEIKIFDDAKITKSESYYITIGDVAKEIGWKGE"
     gene            complement(4989..5363)
                     /locus_tag="VF222_12080"
     CDS             complement(4989..5363)
                     /locus_tag="VF222_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HEX7143205.1"
                     /translation="MEHDEVKTMFAHTLNDDIKWTLRIAYKKTKTPLWKTLEKKIENS
                     RSHKSEINIGKLENLTKDGENIIVPGKVLGNGVLTHKLNIASFSISIPAIKKILDAGG
                     KVITINDFVKQHPDGKGVRIIG"
     gene            complement(5385..6065)
                     /locus_tag="VF222_12085"
     CDS             complement(5385..6065)
                     /locus_tag="VF222_12085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HEX7143206.1"
                     /translation="MDKNLELLLDLSLNILEKTDEKIILKFNNIPRQYLNAIRRIAIS
                     EVPTLAIDDVVILENSSVMHDEAIAHRLGLIPLRTELNRFVFSNHCDCNNTLGCSKCR
                     VLLILDAESSEKTRLVLSGDLVSEDEYVKPVSKSIPIITLAPGQKLKFEAYARLGIGK
                     DHAKWQPTSSAVVKDGNDKDETILIIETTGSLTAEETIKTALKIINSKINNFGDVIKS
                     LEIPSKTR"
     gene            complement(6226..6402)
                     /locus_tag="VF222_12090"
     CDS             complement(6226..6402)
                     /locus_tag="VF222_12090"
                     /inference="COORDINATES: protein motif:HMM:NF025826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HEX7143207.1"
                     /translation="MPTDTKCSICNNPISLFRYKAMKQWNILGYLCNDCYSKKLKEHY
                     SSKKENSNYNKKTD"
     gene            6650..7150
                     /locus_tag="VF222_12095"
     CDS             6650..7150
                     /locus_tag="VF222_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7143208.1"
                     /translation="MGLNEKIIFLGILGMGAIIGVISYTIFSDLPPTPGNTKSIYRQD
                     LPPPPTAQASSKTEPKVDPATVKADVTLTILEGASTQGNPDYDPKELAVKKGNIIKVE
                     NVDSLPHTVTNGAGPNDPNSAKIFDTSLIMGGESGILQTEEIDASEYTYYCTVHPYMT
                     GTLKIE"
     gene            7288..8061
                     /gene="sufC"
                     /locus_tag="VF222_12100"
     CDS             7288..8061
                     /gene="sufC"
                     /locus_tag="VF222_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX7143209.1"
                     /translation="MVFLQVKDLNVSIDGKKILNGLNLEIDKNEVHAIMGVNGSGKST
                     FANVLLGHPKYSVTSGDIIVKGKSILELSTDERAKQGIFLGFQYPIEISGVGYSHFLR
                     NAYNTLNKSLMEDQNDREVFLTVKEFHEYVKKNLNNVGLDPSFLGRYLNEGFSGGEKK
                     RSEVMQMLVLRPTIAILDEPDSGLDIDSVKAVAEAINKLIATGAGVVVITHYARILRY
                     LTKLDKVHVMAKGKIIKSGGKQLSEELETKGYVWLGLEE"
     gene            8122..8904
                     /locus_tag="VF222_12105"
     CDS             8122..8904
                     /locus_tag="VF222_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX7143210.1"
                     /translation="MSFNKNINNKLESSNNNDNSIEKQITPVGNEIKTEDIKKEEQLF
                     LNFSFFKVDPKWRWLNEIGKEEAAKEFESLLEVANTKMKVISYSTIGLRSDADFMLWM
                     IADSVEKMQILTSKIYYTVLGKYVEPSQIYLSSLRRSIYSTQVKLGFMANQKPLKYNV
                     VYPFIKSREWYLLPFEKRKKMMEEHIEVGRKFPNVRLNTSYSFGIHDQDFMLAFETDD
                     LNDFQNLIIKLRETKVSKYVVKDTPMIVCLHKNIGQVIKSLG"
     gene            8917..9621
                     /locus_tag="VF222_12110"
     CDS             8917..9621
                     /locus_tag="VF222_12110"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX7143211.1"
                     /translation="MIALKEWAIICKALEDGKQMLLLRKGGIMEYRKGFEVKHKEFLL
                     YPTFEHQSIESIKTEYKEKLKEILEEQNKYYNDNRKKIDKDNKNFNTVSNNIIKLLAK
                     VEDVIEISDKLTLSELRDYHIWSDEYVTMRMNYNPSKPMSVLLLRIYKIRNPLIVDIN
                     DKWAGCKSWIDIETDREFQNSHKLKMQNADYDTYESINTNKVETLKNEKTIIDPVIND
                     DLFYESANNIRKVIGK"
     gene            9618..10640
                     /locus_tag="VF222_12115"
     CDS             9618..10640
                     /locus_tag="VF222_12115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7143212.1"
                     /translation="MKYRKLGRSGIKVSEIGFGAWIIGLDWWGKKIDDDEAIKMLKKA
                     YDLGINFFESADMYGKGKSEKLIGQVFKDMRNEIVYSTKWGYDMYGHEQIGHNEIPQK
                     HDPEFLQFALEKSMERLQTEYIDVYSLHNPKMDAIKNNDLFYALDGLVTEGIIKSHGI
                     ALGPAIGWEEEGIYTIKNRNITCLQTVYNILEQDPGRSFLNTVIENSSHSIDNENVYD
                     VGIMVRVPDASGLLTGKVTEKTILNQNDHRSYRKKEWIMEALKKIEKMKSIAEYKDWT
                     ISQLAIKFILSQKPISVVMPTILSLEELELFSEMSDGLYLKEEELKQINELYENNFYI
                     HQNIAK"
     gene            complement(10820..11299)
                     /locus_tag="VF222_12120"
     CDS             complement(10820..11299)
                     /locus_tag="VF222_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7143213.1"
                     /translation="MEIPIEDIEIISVVMEEFVDRFHHGKEEKAYFPETQDKDGFSED
                     IRKFLIEHELGRRIAMMVRREINNWKNNEIKTIEPIARFLKSYSVFIDEHTMKEDSFF
                     GMITVKNSISEKEDKKLLKHYELCVNNVSGIERLKQAVKLIEFLENRKWMNDNTIHN"
     gene            complement(11943..>12390)
                     /locus_tag="VF222_12125"
     CDS             complement(11943..>12390)
                     /locus_tag="VF222_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX7143214.1"
                     /translation="RDYYMKMRNVESEGMITVTPRQLEGLVRLATARSKLLLKDYVDS
                     EDAERAIYLIQTMLETAGVDVNTGRVDLGVLHGKPQSEISKLKLFMEVFHGLSGQDKN
                     DVEEKNFINELIQTGRFSEEDAKNFLNKAMQNGQIYERRSGFYAKA"
BASE COUNT         4199 a         1887 c         1757 g         4547 t
ORIGIN      
        1 aggtcctgct gctttataaa tagaatcaga tgtagttgtt ttacttaatt cctccaatgc
       61 tctttctgtt tcaacaattt ctagttcaat ttgttgcttc tgcatcataa tagcttgcag
      121 attttgttga agctgttgta atctagatat ttgttcttta agccaaggag gtagttcatt
      181 ttcttgactc aattcattga ataattaatt aattgaatat aaattcataa cgttatataa
      241 ataacagatt agttatacat aaatgagtag ttttaataag gcgcaagtaa gaattgatag
      301 cagatcttaa ttctgccata tcagaagtag aaatttcaat tattaaatgt gacatacaat
      361 ctttatttga taaatgcaat ttaatttgag gagtgactaa aatttttgta tcaggttcta
      421 atgcattata taaagattct aaaaattttt ttgtttctga aggtgaagaa agatcaagag
      481 aaatattaat tttagctttg aagtttctca atacttatat catatgctcc agcagctact
      541 ttaaaattac attttggaca attccaaatt ccacatgcca ctcgcacaaa ttttaatgaa
      601 ccacaggaag gacaacgtct tttttgttta agagtccgat atactttact atatcttttt
      661 ctaacagtgg caccaaattt tgttcctaga ccctttagta cggtttgacc ttttctgttt
      721 ctatttacca cctattttat cagctccttt aatttagttc gtaaagtctc tgctttaact
      781 cttgctgttt gagccgcaaa ttttatttgt tcaagggtaa aataaccagt tgatcccttc
      841 tgtattgcag catattcatt attagaatta gtagttattg ttatcctgga ttccatacat
      901 gcctcctctt cagcagtagg atctataata atattatttc ctattcggat tgaagttatt
      961 gatacaggta ttgttctaat tggaggttcc tgtttttttc ctgtatcaac caccttttca
     1021 tcacgaattt caaaaattgg tagtttagtg cttaataatg cagcaactac agcataggaa
     1081 gttgcatcaa aaagattgcc atcagcattg attatactac aatcaacgaa aataacataa
     1141 actgttttac caggaattaa aactaattta tctaagtcta tcatctgaga ttctctgatg
     1201 cctcgatcaa ctactctagc aagctctatt gtctcttcgt caggaggtcc aggttctatg
     1261 tagggagatg caataggtag aacttctgca gttacaatta atgcaccttt attttctaat
     1321 ccttcaaatg gtgcgccggt ttcaactttt acacctgcaa ttacttctga attacctaat
     1381 tttaccttag cagaaccatc tgcttttttt attatatcta tgtctatttc tagacttctt
     1441 atatcttcta aatttctacc atcaagtctt tttccattag taattaaatc gaacatctgt
     1501 cgtttacgta attgttctat tactattggt gatttttttg ataaactcaa ttctccttat
     1561 tctcctctaa ttctttctct tcgccttcag gcaaatcatt tccaaagtat ttttctatta
     1621 atgcttttct ttgtatttca taaaccattt tacatccatg aatagctttt tctacacatt
     1681 catcaaattg agattcattt aatattccat ctaattgtaa taaggttatc tgttcaagat
     1741 ttggtaaata tgcaacgggc atatcagcgg ttccttcttt atcttcaata tcattgatat
     1801 caagaactat tttatcattt acttttccag ccgcacatgc tgccaccaag tctcgcatat
     1861 taactccagc atcagctaaa gccactgatg ctgcagcaat tcctgcacat ctggagccac
     1921 catctgcctg taatacttca acaaaaacat caatagcagc tctaggataa tcctctaaca
     1981 ttattgctgg ttctaatgct tcacgcatta cctttgaaat ttcaacttct cttcttgagg
     2041 gagctgggtt ttttctagta tctgttgaaa aaggtgacat atgataccta catcttaaga
     2101 cacatcgatc tggaagagcc atatgtttag gatgaacttc ccttggccca tatactgcta
     2161 ctataatttt gtttttacca aattctatag aggcagatcc atctgcattc tttaaaactc
     2221 ctatagtaat cttaacatct cttaaatcat caatagatcg accgtcagta cgctttccat
     2281 tttcatctat tagaattgtt tgatccataa tctttatcct ctgttactac ttaggttgaa
     2341 cctcttttgg agattcagta ttaatatttg aactatgatc attttccata ttgctggaat
     2401 tattatctgt attgctattg ttgacatagt cttcgttatt cacagaacta ttattagttt
     2461 ctgtttgtgt tatctcctca gaaacattta gcaaatcttt cactttttgt gttaaatttg
     2521 aggtatgtgc ttcttcctca actaatttta tagctttaaa agcaagagca ataccttcac
     2581 cgttttttcc agttacaaca agtatcccat tttgcccaat taaaattcta gtttgtgtag
     2641 cttgttcgat tgtttgtatc atagaacctc tttttcctat taaacgagga acacgtgttg
     2701 aggatatttt taaaaattca ccattagaaa tcttacctaa atccttttcc tgaatggtta
     2761 acattggatc tcttgttctg tcaaaagtta ttattctcgc tgttaccaaa tcacccggcg
     2821 caagtctttt tttcatatca tcttttgcag gagaaaaatc tctcccaaac acatcctgag
     2881 ccggaagatg tgctgcaaaa catgcattaa tatctacttc ccaagataat gaagaattat
     2941 ctacaatttt tccaattact atgtcattta ttctcggaat ataaacacct gacaagggaa
     3001 taacttttat gccatctttg cttgattcgg caatccctat cctagtagca attataaaat
     3061 tattcttttt tattacattc attaatggtc tataatttcc ttctataatt acatcacctg
     3121 gaacaacata tctcttcttg atttctaaca tgaattataa taacatctcc ttattagtaa
     3181 cattaacaaa gaaaagtatc ttgtttgtat cactatataa caattttata tcttttctca
     3241 agttccaatt tcctatttta ataaatggta tttattcatt gttattattg ttcttgaatt
     3301 tgagcagatc cttttgtaat agaatttatt ctttcaatga atgttccgcg tattccagca
     3361 tttatttcta taattacttg taaagaccca tcctgaagcc atttctcatc tttgagatat
     3421 cctatgcctt ttataatact ataagattga gcagaaaatt gtggtggaac ggtaacaatt
     3481 aattgaagat tttctgattt taatggtaaa atttttctaa gagaatcgat aatttttttt
     3541 gcttgttcct cgggtctctt gaagggatct attattaaac gaatttgttc catcgcattt
     3601 tctattctta atggtgggtg aggaaggtgt gtttttggat caataaaatt tctattaata
     3661 tattgaacaa tctgcttttt cttttcttca accattcgcc tacgttgatc agttgtaaga
     3721 ttaacatcac catgttcaag tatttgtttt gcaatttcta tttgatcttt agttttaaaa
     3781 tttttaatta atttctcatt aggaacacgt gtacctttat tagcatctga ataaatttca
     3841 tctgaaacca aaatattaga aatatctatt ttttttccta gcttgtaatc cagtgctggg
     3901 tcaggtttaa ctaaaatttc gaatttatct gcattaagta ttaatcggac tattgagaat
     3961 ttattatcag tcatagaata ttaaaaatga gattttgtaa tatatatgca tacagattta
     4021 atttaattga aatattttca atgtaaaata aaaataataa ctaacggtat gatttctgtt
     4081 tttttcttcg agcacctggc ccaccgaatt tctttgattc agcctgtctg ggatctcctg
     4141 cgagcaaatg tctatcaaat tcagttattt ttttgcgaat ttcttgtctt acactttttg
     4201 acaatggatg atcctttgga tcacgtcctc ctttagcttc tcctgataag gctcgtgata
     4261 ttgctactgc acttgcaaaa gcttgtccca taaatccacc accttcaact tttactgtaa
     4321 tatcaacttt atctcttata tctccaatta tattcacagg agtcaatatc aattcttttg
     4381 agatatcagg ttgtatgatt tctgctggaa tattattaat tctaacttta ccatttcctt
     4441 tggaaatggt agcaacagct ctagacgttt ttctctgacc tggatacaga tctatagtag
     4501 tagttttagt catctcttat tattctcctt tccaacctat ttcttttgca acatctccaa
     4561 ttgtaatgta atatgattca gattttgtta ttttagcatc atcaaaaatt tttatttcct
     4621 ttgaactttg aaattctgtt ggcattccta tatatactct caaacgttta aaggctaaga
     4681 taccgcttgg cttcctcctt gaaaccattc ctctaaccat ttttgtcaat attctatctg
     4741 gtcttcttgg atgataaggt ccatggatgg gattagtaac agaacttact tcaagatatt
     4801 ttttatattc ttcaattgtt ttatatctat ttccggataa cattgctctt tccgaattga
     4861 ttattgctat tctattgcct tgaattaaaa gttttgatac aatagaacat attcgaccgg
     4921 caatacaatt ggaagcatca actattattg gtttattgat atttgaaaca gtattactat
     4981 ttttagattt atccaattat tcttacacct tttccatctg gatgttgctt tacaaaatca
     5041 ttgatggtaa taacttttcc gcctgcatca agtatcttct taattgcagg aattgaaatt
     5101 gagaatgaag ctatatttaa tttatgagta agaaccccat tacctaagac ctttcctgga
     5161 actataatat tttctccatc tttagtaaga ttttctaatt taccgatatt aatttcagat
     5221 ttatgagatc gagaattttc gatctttttt tctaatgttt tccataaagg agttttagtt
     5281 tttttgtatg cgatacgcaa agtccattta atatcatcat ttaaagtatg agcaaacatg
     5341 gttttaacct catcatgttc catattaata atccccaaat aaatctacct agttttagat
     5401 ggtatttcta atgattttat tacatcacca aaattattta ttttagaatt tataattttt
     5461 agtgctgttt ttatagtttc ttctgcagtt aatgaaccgg tagtttcaat tataaggatt
     5521 gtttcgtctt tgtcattgcc atctttaaca acagctgaag aagtaggttg ccactttgca
     5581 tgatcttttc ctatgcctaa tctagcatat gcttcgaatt ttaatttttg cccaggtgca
     5641 agagtaatta ttggtatact tttacttact ggtttcacat attcatcttc tgataccaaa
     5701 tcaccagaca atacaagtcg tgttttttct gaggattcag catctaaaat caacaataca
     5761 cggcatttag aacaacccaa cgtattgtta caatcacaat gatttgaaaa gacaaatcta
     5821 tttaattccg ttcttaaagg aattaatccc aatctatgag ctattgcctc atcatgcata
     5881 accgatgaat tctctagaat aactacatca tcaattgcta atgttggaac ttcactgatt
     5941 gcgatccttc tgatagcatt caaatactgt cttggaatat tattaaattt taatattatt
     6001 ttttcatctg tcttttccaa aatatttaaa gataaatcca atagtaattc aagatttttg
     6061 tccaatgcac tcttaaaaat ctaccgacta ctacaatata aatagggtta ggatatcaat
     6121 aggttctaac gtaattaatt taaattgaat aattcatcga tgttaattct gaagacataa
     6181 ttcattcaat atactaatca taaagattag taggtaaatt attaattaat cagttttttt
     6241 attatagtta ctattttctt ttttagaaga atagtgttct tttaactttt tagaataaca
     6301 atcattacat aaatatccta ggatattcca ttgtttcatt gctttatatc taaatagaga
     6361 aattggattg ttacagatag aacattttgt atcagtaggc attttctttc attattttta
     6421 gaattataaa tataataata attgcatcgt tttaaagtct attattataa atgaaatact
     6481 tcttaattaa tagcctataa tttataatat tttttcgtta tcttcatttt caatttaaag
     6541 atataataat tttgatttac aaaagtaatg aataattaaa atttgctcaa acttgattgt
     6601 ttatataaaa aaattaatta agaccttact cgaattcatg attagttaaa tgggacttaa
     6661 tgaaaagatt atttttctag gaatattagg aatgggtgcg attataggag taatttcata
     6721 tactatattt tctgatcttc cacctacacc cggcaataca aaatcaatct atagacaaga
     6781 tcttcctcca ccaccgacag cacaagctag ttcaaagaca gaacctaaag tagatcctgc
     6841 aactgttaaa gcggatgtaa cattaacaat tcttgaaggg gcttcaacac aaggtaatcc
     6901 ggactatgac ccaaaagaac ttgcagttaa aaaaggcaat ataataaaag tcgaaaacgt
     6961 tgactcgctt cctcatacag ttacaaatgg tgcaggaccc aacgatccaa attcagcaaa
     7021 aatattcgat acgagtttaa ttatgggagg agaatcagga attttacaaa ctgaagaaat
     7081 tgatgcaagt gaatatacat attattgtac agttcatcca tatatgacag gtacgttaaa
     7141 gattgagtaa ctgataaata accagacact ttatttttat attattaaac attattagat
     7201 tttcaaggtt atacatgttt ttttagatat tttatctaaa ttttcagata aggatgcttt
     7261 atatgtaatt aataagaata ttagactatg gtttttttac aggtaaaaga tctgaatgta
     7321 agtatcgatg gaaaaaagat attaaatggt ttaaatctag aaattgataa aaatgaagta
     7381 catgctatta tgggtgtaaa tggatcaggt aaaagtacct ttgcaaatgt actcttagga
     7441 catccaaaat attctgttac ttctggagac attattgtta aaggaaaaag catcttagaa
     7501 ttatctactg acgaaagagc caaacaagga atatttttag gatttcaata ccctattgag
     7561 atttcaggtg taggttatag tcatttttta agaaatgcct ataatacttt aaataagtct
     7621 ctaatggaag atcaaaatga tagagaagtt ttccttaccg ttaaagagtt tcatgaatat
     7681 gttaaaaaaa atctaaataa tgtgggctta gatccatcat ttctcggaag atatttaaat
     7741 gaaggttttt ctggaggaga aaagaaaaga tctgaagtta tgcaaatgtt ggtattaagg
     7801 ccaactatag ctatattaga tgagccagac tcaggtttag atattgattc agtaaaagca
     7861 gttgccgagg caataaataa attgattgcc acaggagctg gagttgttgt aattacacac
     7921 tatgctagaa ttttacggta tcttacaaaa cttgataaag ttcatgtaat ggcaaaaggg
     7981 aaaattatta aatctggcgg aaaacaatta tcagaagaac tagaaactaa aggttatgta
     8041 tggttaggtt tagaagaata aagttttggt ataattttaa ttttagatat ataatacggc
     8101 taattctttt tttatatatt aatgtcattt aataagaata taaacaataa attagaatca
     8161 tctaataata atgataattc tatagaaaag caaattacac cagtaggaaa tgaaataaaa
     8221 accgaagata taaaaaaaga agagcagctt ttcctaaatt tttccttttt taaagtagat
     8281 ccaaaatgga gatggttaaa tgaaatagga aaggaagaag cagctaaaga atttgaaagc
     8341 ttattggagg tcgcaaatac aaaaatgaaa gtcatttcat attcaacaat aggtctaaga
     8401 agcgatgcag atttcatgtt atggatgatt gcagattctg ttgagaaaat gcagatttta
     8461 acatcaaaga tttattatac tgtactagga aaatatgttg aaccttctca aatctattta
     8521 tctagtttaa gaagatcaat ttattctact caagtcaagt tgggttttat ggccaatcaa
     8581 aaaccactta aatataacgt agtatatcca tttataaaat caagagaatg gtatcttctc
     8641 ccttttgaaa aaaggaaaaa aatgatggaa gaacacatag aagttggaag aaaatttccg
     8701 aatgttagac taaatacatc gtattccttt ggaattcatg atcaggattt catgctcgct
     8761 tttgaaacag atgatctgaa tgattttcaa aacttgatta taaaattacg tgaaactaaa
     8821 gttagcaagt atgttgttaa agatactcct atgattgtct gtctccataa aaatattgga
     8881 caagttataa aaagtttagg atgaatgaat atattgatga tcgcacttaa agaatgggct
     8941 attatttgta aggctctaga agatggaaaa cagatgttat tgcttcgtaa gggcggaatt
     9001 atggaatata gaaaaggatt tgaggttaaa cataaagaat ttttacttta tcctacattt
     9061 gaacaccaat ctattgaatc aattaaaacc gaatataaag aaaaactaaa ggaaatatta
     9121 gaagaacaaa ataaatatta caacgacaac cgaaaaaaaa tcgataaaga taacaagaat
     9181 tttaacactg taagtaataa tattattaaa ttgcttgcaa aagtagaaga tgttatagag
     9241 atttcagata aattaacact ttcagagtta agagattatc atatatggag tgacgaatat
     9301 gttactatgc gaatgaatta taatccaagt aaaccaatga gtgttttatt attacgaatt
     9361 tataaaataa ggaacccgtt aattgttgat attaatgata agtgggctgg atgcaaatca
     9421 tggatagata ttgaaacaga tagagagttt cagaattctc acaaattaaa gatgcagaat
     9481 gcagattatg atacttatga atcaataaat actaacaaag tagagacatt aaaaaacgaa
     9541 aaaactataa tagaccctgt aataaatgat gatttatttt atgaatctgc taataacatt
     9601 aggaaggtaa ttggaaaatg aaatatagga aattaggtag aagtggaata aaagtcagtg
     9661 agattggatt tggtgcctgg atcataggac tagactggtg gggaaaaaag atagatgacg
     9721 acgaagcaat aaagatgtta aaaaaggcat atgatttagg aattaacttc tttgaaagtg
     9781 ctgatatgta tggaaaaggt aaaagcgaaa aactaattgg ccaagtattt aaagacatga
     9841 gaaatgaaat agtttattct accaaatggg ggtatgatat gtatggccat gagcaaatag
     9901 gacataacga aatccctcaa aagcatgatc ctgaattctt gcaatttgca ttggaaaaaa
     9961 gtatggaaag attacaaaca gagtacatag atgtttatag cttacataat ccaaaaatgg
    10021 atgcaattaa aaataatgat ttattttatg cattggatgg cctcgttact gaagggatta
    10081 ttaaaagcca cggaatagca ctaggtccag ctataggatg ggaagaagaa ggcatataca
    10141 caataaaaaa tagaaatata acatgtctac aaacagttta taatatttta gagcaagacc
    10201 caggacgttc ttttttgaat actgtaattg aaaattcatc tcattccata gataatgaaa
    10261 atgtatatga tgtgggtata atggtcaggg ttcctgatgc ttcaggatta ttaacaggca
    10321 aagtaacaga gaaaactatt ttgaatcaaa acgatcatag aagttataga aaaaaagaat
    10381 ggataatgga agcattaaag aaaatagaga aaatgaaatc aattgcagaa tataaagatt
    10441 ggacgatttc acaattagcg atcaaattta ttttatctca aaaacctatt tctgtagtaa
    10501 tgcctactat attaagttta gaagaattag aattattttc agaaatgtca gatggtttat
    10561 atctaaaaga agaggaactt aaacaaatta atgaattata tgaaaataat ttttatatcc
    10621 atcaaaatat tgcaaaatag tgattacatt tttcctataa tattttttta aacaaatatg
    10681 tatgtaaata attttaatgg aatggcaata acatataaca cattgtcaat agtttaggat
    10741 aactaatgta caaagtagtt aaacacctta gtaaaaatgc atatatataa ttcagagaga
    10801 attactaaac tacacaatac taattatgaa tagtattatc attcatccat tttctatttt
    10861 ctaggaactc tattaattta accgcttgct tcaacctttc tattccacta acattattta
    10921 cacacaattc ataatgtttc aaaagttttt tatcttcctt ctctgaaata ctatttttaa
    10981 ctgtaatcat accaaaaaac gagtcttctt tcatagtatg ctcatctatg aagacagaat
    11041 aagatttaag aaatcgtgca ataggttcta ttgtcttgat ttcattattt ttccaattat
    11101 taatttctct tcttaccatc attgcaattc tccttcctaa ttcatgttcg atcaaaaatt
    11161 ttcttatatc ttctgaaaaa ccatccttat cttgagtttc tggaaaataa gctttttctt
    11221 ctttcccatg atgaaaccta tctacaaatt cttccattac tacagaaata atctcaatgt
    11281 cttcaattgg aatttctata tttgcaaaaa aagtttctca gaacacactt tagccacatt
    11341 tcttaacctt cttattaaaa tgtgatcttc tttgaggttt ttggttgctc tgttattcaa
    11401 tcttaaataa atttagagtg attatataaa aaagttaaag atatatacat aaagattatg
    11461 aacttcggat atcattcaat tataatattt cctagaaaat aaagtaataa ttgtatctta
    11521 ttaatattaa ataagtgtat ttgtaataat atcattagaa tgtctataaa atattataat
    11581 tcttgctgct tttattaaat ttagtcttaa ttatacctat tttctaatta aattgtaata
    11641 gctaatccca ttaaataact ttttattata ataatttaat atagcatata ataatttaat
    11701 atagcatttt tttttaaata atttattcaa ttaaaattac tggtatcaag tttatcaaat
    11761 tatttctgca tgtattttgt tgtattcaat ataacaattt tttgccatgt taataaagtg
    11821 agacattgat ttgatttata actttataaa taatggtata tttgctatta aaatatctaa
    11881 atctcatata ttgtttatat tgatgtgtac aataatatga attaatgatt tttgattttt
    11941 atttatgcct ttgcataaaa gcctgaacgt cgttcatata tttgtccatt ttgcatagct
    12001 ttatttaaaa agtttttagc atcctcttct gaaaatcttc ctgtttgaat aagttcattt
    12061 ataaaatttt tttcttctac atcattttta tcttgccccg ataaaccatg aaaaacttcc
    12121 atgaatagtt tcaattttga tatttcactt tgaggtttac catgtaaaac tccaagatct
    12181 actcttccag tattaacatc tactcctgcc gtttctagca tagtttgaat taaatatatt
    12241 gcacgttcgg catcttccga atctacataa tcttttaata ataattttga acgggcggtt
    12301 gctaatctga ctaatccttc aagttgcctt ggtgtaacag taatcatacc ctctgattcg
    12361 acatttctca ttttcatata ataatctctt
//
LOCUS       DASVEV010000277         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1121513, whole genome shotgun sequence.
ACCESSION   DASVEV010000277 DASVEV010000000
VERSION     DASVEV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121513"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1130)
                     /locus_tag="VF222_12130"
     CDS             complement(156..1130)
                     /locus_tag="VF222_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX7143215.1"
                     /translation="MTKLKFGLTIPQGWWSDLPSNDPIKQYEFSKKISRSADRLNFYG
                     LYAYDHFIPHHEYNINNNFFECFTLLSSISSITKKIRLGQIVTCNSYRNPALLAKVLS
                     TLDVISKGRVELGIGAGWYQNEYSSYGYNFPSNVERIKQLDESISIIKEMWKYERASF
                     HGNYFSINEAICKPHPIQKPHPRIIVGGTGEKYLLKIVAKHADTYNNPFGSPLEIRKK
                     LTVLKEHCNDLRKNYNDIELSVLLPCIIQESEKDVLMEINKWKKKNENISAFLERKSG
                     GYTIGTPEKILLGLQQYIDLGITHFILNFIGLEEGMLHLFDSRVIKKL"
     gene            complement(1158..2756)
                     /locus_tag="VF222_12135"
     CDS             complement(1158..2756)
                     /locus_tag="VF222_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX7143216.1"
                     /translation="MNQNIKIAKEFHESTKHSPITIYFNRHYLDWENKPFPFKIYLNK
                     PKIDLPSTFPYPIEDSIECLKKQMNLNLSEQKQIDPKILSQILFFSAGITRKMKLNDN
                     VYYMRAASATGALYPIEIYLICQKIQNLDAGVYHFCPADFTLTQLRKGDFRSILFTAT
                     GNNNKVIQAPITLIFTSIAWRNSWKYQARSYRHWFWDAGVILANFLALCNNNLLSSEI
                     ILGYQDNLINNLLGLQNQKEAAIALASIESYYNYDDPKQFNSSQLSPINCETKPISKY
                     EETVYPDIWKIHESSYLNTPIEITNWTLKNKEDVNLHNTRKISYDKINTINKYNIGDI
                     ILQRGSTRRFSKKPITLTQLQNILSLSSISIPFDFITNEQSLIDIYFIANYVTNLQSG
                     IYYYNKLENNIEELKKGDFREKSGHLCLDQNLFSNASVVFFLLASMDTILKYLGNRGY
                     RAVQLEGGICTGKIYLASYSQGLGASGSTFYDDEVTETFLPHSNQKSTIIAIGIGIPA
                     YVSKPGTIYTNVLNTEDYSKKFNY"
     gene            complement(2786..>3180)
                     /locus_tag="VF222_12140"
     CDS             complement(2786..>3180)
                     /locus_tag="VF222_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143217.1"
                     /translation="NATEGAENATEGAREQIKESLSSAQSGAANATEGAENATEGAEN
                     ATEGAENATEGAENATEGAREQIKESLSSAQSGAANATEGAENATEGAQSAAENATEG
                     AQRTGEDPIEIGKNFLDQIWGKVTNLFK"
BASE COUNT          978 a          488 c          487 g         1227 t
ORIGIN      
        1 ataaaggtgt atttgtaaaa actattgcta aatatataat tattataaca agatctaact
       61 atttacttaa tattatataa tatttatagt tattatatga gatttaggat gaatatgaat
      121 taatcgttga tgataatcat aagtgattat caagatcata gtttcttgat aaccctagaa
      181 tcaaataaat gcagcatacc ttcttctaaa cctatgaaat ttagaataaa atgtgttatc
      241 cctaaatcaa tatattgttg aagccctaac aaaatttttt cgggtgttcc aattgtataa
      301 cctccgcttt ttctttccaa aaaagcagaa atattttcat tctttttctt ccatttattt
      361 atttccatta gaacatcttt ttctgattct tgaataatac atggaagtaa tacggacaac
      421 tcgatatcgt tataattttt tctcaagtca ttacaatgtt cttttaaaac tgtaagtttt
      481 tttcttatct cgagtggact accaaacgga ttattatatg tatcagcatg ctttgctact
      541 attttcaaga ggtatttttc tccggttcca cctacaatga ttcgaggatg aggtttttgt
      601 atagggtgag gcttgcaaat agcttcatta attgaaaaat aattaccatg aaatgaggct
      661 ctttcatatt tccacatctc ctttattatg gatatggatt catcgagttg tttgattctt
      721 tctacgttag aaggaaagtt atatccatat gaggaatatt cattttgata ccaaccagct
      781 cctattccca attcaacacg tcctttggaa ataacatcca gagttgaaag aacctttgct
      841 aataatgcag gattacggta ggaattgcaa gtaacaattt gtcccaatct aatttttttg
      901 gtgatagaag aaattgaaga taataatgtg aaacattcaa aaaaattatt atttatgtta
      961 tattcatgat gtggaataaa atgatcatag gcatataaac catagaaatt tagtctatct
     1021 gctgatcttg aaattttttt agaaaattca tattgtttta taggatcatt tgacggtaga
     1081 tctgaccacc agccctgtgg gattgtcaat ccaaacttta atttggtcat tattgttatt
     1141 ctttgttatt actgttgtta ataattaaat tttttagaat agtcttctgt gtttaacacg
     1201 ttagtatata tcgttccagg tttagataca taagcaggaa tacctatacc tatagctatt
     1261 attgtacttt tttgatttga atgaggaagg aaagtttctg ttacttcatc atcataaaag
     1321 gttgaaccgg aagcgcccaa tccttgagaa tatgaagcaa gatatatttt tccagtacat
     1381 atgcctcctt ctagttggac agctcgataa cctctatttc ctaaatattt taatatcgta
     1441 tccatacttg ctaaaagaaa aaataccaca cttgcattac taaataagtt ttgatctaga
     1501 cacaaatgcc ctgacttttc cctaaaatca ccttttttga gttcttctat gttattttct
     1561 aatttattgt aataatatat accagattgt agatttgtga cataatttgc tataaagtaa
     1621 atatcaatca ggctttgttc attggtaata aaatcaaaag gtatgctgat actagaaaga
     1681 gataaaatat tttgtaattg agttagggtt atgggctttt ttgaaaatct tcttgttgat
     1741 ccacgttgta aaattatgtc cccaatattg tacttgttaa tagtattaat tttatcataa
     1801 gaaatttttc gtgtgttgtg taaattcaca tcttctttat ttttcaaagt ccagtttgtt
     1861 atttctattg gggtattgag ataagaagat tcatgaattt tccatatatc tgggtaaacc
     1921 gtttcttcat attttgaaat gggtttggtt tcacaattta taggtgaaag ttgactggaa
     1981 ttaaattgtt tagggtcatc ataattgtaa taactttcaa ttgaagctaa tgctatagca
     2041 gcttcttttt gattctgtaa tcctagtaaa ttattaatga ggttatcttg atatcctaga
     2101 ataatttcgg aagataaaag attattgtta cataaagcca aaaagttggc gagaattacc
     2161 cctgcatccc aaaaccagtg tcgatatgaa cgtgcttgat atttccatga atttctccaa
     2221 gctattgaag taaaaattaa cgtaattgga gcttgaatta ctttattatt atttcctgtg
     2281 gctgtaaata gtattgatct aaaatcgcct tttcttaact gtgttaatgt aaaatctgct
     2341 ggacaaaagt gatatacacc tgcatcgaga ttttggatct tttgacaaat taaataaatt
     2401 tcaatggggt ataaagcacc ggtagctgag gctgccctca tatagtatac attatcattt
     2461 aatttcattt ttcgtgtaat tcctgcagaa aaaaagagta tctgggataa gatttttgga
     2521 tctatttgtt tctgctcaga tagattcaaa ttcatttgtt ttttcaaaca ttcaatagaa
     2581 tcttcaatcg gatagggaaa agttgatggt aaatctattt ttggtttatt taaatatatt
     2641 ttaaatggaa atggtttatt ttcccaatcc agataatgac gattaaaata gatagttata
     2701 ggagaatgtt ttgtgctttc atgaaactct tttgcaattt ttatattttg attcaaggat
     2761 ataggctaaa ttaatatctt aaaaattatt taaagaggtt tgttactttt ccccatatct
     2821 gatctaagaa atttttccct atttcaatgg gatcttcacc agttctttgt gcaccttctg
     2881 tagcattttc agctgcactt tgtgcacctt ctgtagcatt ttcagcacct tctgtagcat
     2941 tagctgctcc actttgtgca ctagatagtg attcttttat ttgctctctt gcaccttctg
     3001 tagcattttc agcaccttct gtagcatttt cagcaccttc tgtagcattt tcagcacctt
     3061 ctgtagcatt ttcagcacct tctgtagcat tagctgctcc actttgtgca ctagatagtg
     3121 attcttttat ttgctctctt gcaccttctg tagcattttc agcaccttct gtagcatttt
//
LOCUS       DASVEV010000278        14247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1121538, whole genome shotgun sequence.
ACCESSION   DASVEV010000278 DASVEV010000000
VERSION     DASVEV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121538"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..747
                     /locus_tag="VF222_12145"
     CDS             604..747
                     /locus_tag="VF222_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143218.1"
                     /translation="MCDNCKCRVINAMQERIEESRPFIKHMDRKDPSEEIDDVSFRLL
                     VLP"
     gene            1052..1198
                     /locus_tag="VF222_12150"
     CDS             1052..1198
                     /locus_tag="VF222_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143219.1"
                     /translation="MTSLVFCSKCGKTEQPGEEFTNKDGSTICKECWVDDEENNNNNN
                     KDKK"
     gene            1195..1449
                     /locus_tag="VF222_12155"
     CDS             1195..1449
                     /locus_tag="VF222_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143220.1"
                     /translation="MSSTNNNKKVLYQLWTNGKGSFAVGKIGSQLLEYSDSQYTIDKI
                     LDSKMLYDGSITEELVQDWIQELKQEDILDENGNLIYVTF"
     gene            1714..1944
                     /locus_tag="VF222_12160"
     CDS             1714..1944
                     /locus_tag="VF222_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143221.1"
                     /translation="MSNSFELLTGPIDSIQKKYEILSDIVTEYGGKVHGSQSHIVETN
                     NSIQLIVYYEVPEGKRDEVKQKLITEKNKIIL"
     gene            1986..2378
                     /locus_tag="VF222_12165"
     CDS             1986..2378
                     /locus_tag="VF222_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143222.1"
                     /translation="MTEKYGLPFEIVESKDTIDKDKNKIKAGLKKIEIFASPEQVNEI
                     ISVIESLKLEATLYDSHGYGQSKQKIRSGKAGGQTATISSSRKTIVTIAESDILEELV
                     KKLKHINDKSEKKIGVISIQPVDTLVHL"
     gene            2587..2988
                     /locus_tag="VF222_12170"
     CDS             2587..2988
                     /locus_tag="VF222_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143223.1"
                     /translation="MKKNILIVDDDIKTSLQYKKWLEDEGFNLTLINDPNIAEQNFNP
                     ENYDLVLIGFRMSVVDGFDLYHKLHDLSKKVQQDTSPSNDFRICFMTSSRINYTALAE
                     VHPEFGEECYVSKEVPKDVFIQHVHSLLLPG"
     gene            3005..3196
                     /locus_tag="VF222_12175"
     CDS             3005..3196
                     /locus_tag="VF222_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143224.1"
                     /translation="MSTQNNNNNKECKHTVLSIKDRSCTVCGMSRYDQLQNTEKGLTK
                     AEEKELEGYINRKHWELID"
     gene            3211..3570
                     /locus_tag="VF222_12180"
     CDS             3211..3570
                     /locus_tag="VF222_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143225.1"
                     /translation="MASNNNNQNINPRPCVYGCGIQIYWNASVNEYWEVLTKKKHICP
                     NRVNKSVTNTTNSPTSGVSNKPPYSNKKPLSPTPKPKMSNSFEYLQGSIAEIQRKYEI
                     LSDLVTEHNGKVQGSQG"
     gene            complement(3708..4130)
                     /locus_tag="VF222_12185"
     CDS             complement(3708..4130)
                     /locus_tag="VF222_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143226.1"
                     /translation="MDFKVSVENEDHDTRILAPWFLNCLNRLSESNPAKFILKQQVFA
                     QTPMNGFKADPEKFQKYVDLKGNPDNASVFEPVIHFLERNRFLRISSVNPAKIAITRI
                     GVDKCNEPTDIEWSYNHTFYPIEDNDDIAKNPGYRNYK"
     gene            4487..4915
                     /locus_tag="VF222_12190"
     CDS             4487..4915
                     /locus_tag="VF222_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143227.1"
                     /translation="MPKGFFAPTVPLNPIVPPLNLILFPFFQDAEASSGLDEIKDITK
                     SMKRSVEKMEEQGECAEENLEKTLFYSKKGKIYHGNPDCDPSKQYRYDRLNKMSGIDE
                     YKDAHNFILSNKNYFQAKNEDDETSNRTYKGFNIIAFYYC"
     gene            complement(4944..6332)
                     /locus_tag="VF222_12195"
     CDS             complement(4944..6332)
                     /locus_tag="VF222_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143228.1"
                     /translation="MDVPGRREDDDLLIFQDQEEEDKKDPFSSFMYGLKAPETRRQWP
                     ARLKKFFDFGINPKLDLQKQAVIFYEKASINEKWTVLYIEKFIEFQKERVVKREIEST
                     TIRNYYKAAKLFCDMNDITLNWKRLSKGLPKQKQASDDRAPTLEEIKKLMDYPDKRIK
                     PILLTMLSSGIRKETWDYLKWKHIVPLFNNHDNTIIIAAKIIVYAGDNEEYFSFITPE
                     AYNSLKEWMDFRAFHGERITGNSWVMRNLFTVSERTWKRDPNHSNYLGDINEPVHLSS
                     DGVKSMIERAIHAQGLWSPLKKGSRRREWKGMHGFRKFFKTQAEHVMKSLHVEMMLGH
                     NTGLAKNYYRPSERELLEEYIKAVDDLTINDENRLSKQVKELKEKNQDSEYVIKGKLQ
                     EKEEQIKSLEESIKFLSDRFNAFLISQPENKVIHDNEQTGIIKGIELKPDLNNKAVGE
                     IAIPSSSNNKKK"
     gene            complement(6373..6446)
                     /locus_tag="VF222_12200"
     tRNA            complement(6373..6446)
                     /locus_tag="VF222_12200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6410..6412),aa:Ile,seq:cat)
     gene            complement(6516..7232)
                     /locus_tag="VF222_12205"
     CDS             complement(6516..7232)
                     /locus_tag="VF222_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX7143229.1"
                     /translation="MENNHKDSGNVGLLIKIIDDEISKRSLLNHQFYTLWKEGKLTLN
                     HLRSYSKEYFQLVKAVPQFVDNIHKNLHNSDPGNEYVKPLKNTQSEEYQHIEPWIKFA
                     QSLGVEKKELLEYEGNNEVNLAIENLNELCFSSICEGASAMYSFEKELPQISKTKVEG
                     LVRFYNISSDEDIKYFKIHQIADIEHAKLWRTILLDNKLVGNTKDVNKNILNASVSSM
                     NSQNQILDTVYNNYVKDTMN"
     gene            7339..7436
                     /locus_tag="VF222_12210"
     tRNA            join(7339..7353,7376..7436)
                     /locus_tag="VF222_12210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7397..7399,aa:Glu,seq:ttc)
     gene            7540..8487
                     /locus_tag="VF222_12215"
     CDS             7540..8487
                     /locus_tag="VF222_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7143230.1"
                     /translation="MDGDTIKGKNTNQESSSSLMEQFGDFLLKINRTLERELDLYSWS
                     EFYSSLKYACDGGKRIRPLILLLSSEIIKSKNNDNTDLLNTPQAFSKRDDERFLAASA
                     IELLHTESVIHDDIIDEENVRRGKPSFHIKYGYNNSILTADFVLGIILNITSKLNNPR
                     ISSEIANAAIRMSEGEIMEIRLNKDHKISEDDYIKVLEHKTASLFESSTRIGAILAHA
                     DEQDTRAMGNFGHLMGIAYQIHDDLDDWNNEDRLFNLLMKQNNHSQHLVSKMENLYKS
                     YSLKAKNELNLVISNIKNNYTERLKHQLEELTNLNMLPS"
     gene            complement(8903..9343)
                     /locus_tag="VF222_12220"
     CDS             complement(8903..9343)
                     /locus_tag="VF222_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX7143231.1"
                     /translation="MTKLRIASFIPATEVRTYLSDRSYGGGYSGGRRNSSGGGYGDRN
                     RPRRFRHSPVKVGEQYDVTIEAISNRGDSGVAKIEGFVIFVPGTKVDEKIKVKITKVG
                     NGYATGELAQQTSDQTGSENTEQLQEDTKITEEGEESEELEEKS"
     gene            9786..10142
                     /locus_tag="VF222_12225"
     CDS             9786..10142
                     /locus_tag="VF222_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7143232.1"
                     /translation="MLHKICDVNTIKNNSMKVFHLGLDSKVIDILIGKINGKLFACDN
                     YCPHRGASLSKSGISSDNQRIICYLHDFEYNVNTGKLENIPSKWLTQNPEWKKSNDLV
                     IYKIIEKEKNIYVDIP"
     gene            complement(10195..10512)
                     /locus_tag="VF222_12230"
     CDS             complement(10195..10512)
                     /locus_tag="VF222_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143233.1"
                     /translation="MLAKITSYTKNIVRITQLDIDEYGSIIPLGEIELTFSANNRPLY
                     ELIKNSTYAAIFTKKDVPSEGPLECTLDENSNNIILVIPMTLDELNDEKKKVIESAAR
                     FQK"
     gene            complement(10762..11073)
                     /locus_tag="VF222_12235"
     CDS             complement(10762..11073)
                     /locus_tag="VF222_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143234.1"
                     /translation="MKNIFSAIAFFILHQGKQRRNIIILGGSLLAIGFIGMITSGSIV
                     IQDTVNQNLTPNGYPKTAILGISVYSYEYASLFITILGVFVFTGGMVAMDSKIKQKSH
                     F"
     gene            complement(11335..11916)
                     /locus_tag="VF222_12240"
     CDS             complement(11335..11916)
                     /locus_tag="VF222_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143235.1"
                     /translation="MVSGRYFNRGVKTVDNPDIGYVVRETPDKIIVFGGKGNRYDIPL
                     SEIQQVGANVLIGLPFSEVEKKYKVNRNDPLPTSRPDPWKDEKKQVDLAYYEGKYPKS
                     LFNKGVRAENEDDLGFVAKETNDKIVVFGYSNDRYDIPKSDIIAVGMNVIIGKNFPEL
                     SKYKVDRNAPLPTGEPIETIAEEAFPEKSSEPT"
     gene            complement(12640..14190)
                     /locus_tag="VF222_12245"
     CDS             complement(12640..14190)
                     /locus_tag="VF222_12245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7143236.1"
                     /translation="MIDISSTPIIIIILLGSVGLGIPIIDAYKKEKGKSRNQLFSAIT
                     FGTLIIALLIVISRVLSGEPLPAMSFGNDVIADDMFGSFFAVSLLIVSIMVSASSWNY
                     MKGISNYSSYYSLILLSTIGMILIAYSTDFVMLFIAWELMSIPTYALAAFSKKDPISN
                     EAAIKYFMFGAFSSSILVLAIGFVYAVTGTTNIGDSITILAELSVPNQTPDNNNLGGV
                     IPISYLAIGLFVAGFGFKMGLVPFHMWLPDAYEGAPTTIGALLAAGTKKAGFAAAIRV
                     IVIGMFAIGLDWSIVLAILAVITMTVGNFGALIQKSVPRMMAYSSVAQAGYMMIGLAL
                     APYASSALDGSLFHILNHAVMKSASFLAIAAVAIALASYSLEKYSGLGRKMPITAIAL
                     SISLLALAGVPPLNGFWSKLVLFESAIDSGTVISWGPYLAIAGLLNSALSLGYYLWVI
                     KKMYLDESGDMSRVKEPKAILGVLIFAIIFMVGFGIWHAPLLEFASISVPSHIDQLAP
                     SFGNITNN"
BASE COUNT         4991 a         2210 c         2147 g         4899 t
ORIGIN      
        1 agaacgaata tattgaaaaa tattgtcact atattttaac attgactcta tatagaaata
       61 gaaatatttt tttcttctgt tattgttatg gttatacttt cttgtcactc ttaaaatcct
      121 attcaaaaaa tcattttggg gttctggcag atcttgattc ctttactata tcgctagagt
      181 aataaattaa ttatattgcc atagcctgaa cttcataaat aagtggactg gtggaattaa
      241 tggctcaaag tcgcttagtt agtcatccag accatgacca taatgccggt aggttcatat
      301 tgttttcgaa atggccctaa aggtcacaat attgtttaca atactaaccc aactctatga
      361 ctgttaacaa taaatttaga agttttattt tttgggtata tctatttcta ttgtttgtaa
      421 tttacttgga ggaacacaat tgagacaaaa gattttcaag ttgtcgtatt gaacatgaca
      481 gtgtggacat tttttcatta aatcattaat tattaagaaa tctactataa gaatagtatg
      541 aaaatattta cctgtactgg ttcagtagac cttgagattg aggtcgtaga tacagatcac
      601 gatatgtgcg acaactgtaa atgtagagtc ataaacgcta tgcaagagag aatagaagaa
      661 tcaagacctt tcatcaagca tatggatagg aaggatccat cggaagagat agacgatgtg
      721 tctttcagat tgcttgtttt gccatgacct gatacgaaca gactaatttg ataagttcta
      781 cgggataata tcgctagcgt taaattataa aattatatat atataattta aattataaaa
      841 aaaaatttaa taataataat aatttaataa tataataaca aaaataataa aattgaaatt
      901 ttagttgtgg ctacaagttt caaagcaatg aagtcgaatt gacaaatgta gacatttctg
      961 ataaatattt tagaataaat tggaactgtc cacaatgtaa tacagaagga ttgacaacgt
     1021 ttatcaataa taataaaaaa tatgatatga gatgacttca ttagtatttt gttcaaagtg
     1081 tggtaagact gaacaaccag gagaagaatt cacaaacaaa gacggtagca cgatatgcaa
     1141 agaatgctgg gttgatgatg aagagaataa taataataat aataaagata agaaatgagt
     1201 tctactaata ataataaaaa agtattgtat caactgtgga ccaatggcaa gggtagtttt
     1261 gcagtaggga aaataggatc acaattactt gaatattcag attctcaata taccattgat
     1321 aaaatactag attcaaaaat gctttatgac ggttcaataa ctgaggaatt agtccaggat
     1381 tggattcagg aactcaagca agaggatatt ttagatgaga atggaaacct gatctatgtc
     1441 acattttaac actacttcta atcaaaataa tactatttat cccaaatcct gtggttatgg
     1501 atgcggattg caaatctact ggaatacttc tgaaaacact tactatgagg tattcagtaa
     1561 gaaaaaacac atttgtccaa acttttggta ataataagaa accagtaagt tgctaatact
     1621 tccaataata tagcaggaac tgcagcaaca actaatacta aacccaccta ttataataaa
     1681 aaaccctgga attcaaatca accaaaaccg aaaatgtcta attcatttga attactaact
     1741 ggtccaattg atagcattca aaagaagtat gagatattat ctgatattgt tactgagtat
     1801 ggcggtaagg tacatggttc tcaatctcat attgttgaaa caaataatag tatacaatta
     1861 attgtttact atgaagtgcc agaagggaag agagacgaag taaagcagaa attgattact
     1921 gaaaaaaata aaattattct ataaacaaat actttatctg tatagtacat aatatattgt
     1981 ttctaatgac agaaaaatat ggtttaccat ttgaaatagt cgaatccaaa gacacaatag
     2041 ataaagataa aaataaaatt aaagcaggat taaaaaaaat agaaatattt gctagccccg
     2101 aacaagttaa tgaaataatc tcagtaattg agagtttaaa gttagaagca acattgtatg
     2161 actctcatgg ttatgggcag tctaagcaga agataaggtc gggaaaagcc ggaggacaaa
     2221 ctgccacgat atcatcgagt cgtaaaacta tagtaactat agcagaatct gacattctag
     2281 aagagttagt caaaaaactc aaacacataa atgataagag tgagaaaaaa attggagtaa
     2341 tatctattca accagtcgat accttagtac atctatagtt attactacat catcgtatca
     2401 tcaacatatc atcatgtctc atattttttt tccttccttc cttctttttt cttataatat
     2461 gtataaattt caagttcatg cttctaaaaa ggcaagcggg aagaagtaaa acgaaagttt
     2521 gagaacttgg ttagaaataa taagtaaaat ttatcagcat caataacaat aactaatata
     2581 tcaacaatga agaaaaatat tctaatagtt gatgatgata ttaaaacttc tttacaatac
     2641 aaaaaatggt tagaagatga aggcttcaat ttaactttaa taaatgaccc taatatagca
     2701 gaacaaaact ttaatcctga aaactatgac cttgtactta ttggttttag gatgtctgta
     2761 gttgatggat ttgatcttta tcacaagtta catgatttgt caaaaaaagt acaacaagat
     2821 acatcaccat caaacgattt tcggatatgt tttatgactt catcaagaat aaattataca
     2881 gcgttagcag aggttcatcc agaatttgga gaagaatgtt atgtttctaa agaagtacca
     2941 aaagatgttt ttatacaaca tgtccattcg ctactactac ctggataaat aatgacaata
     3001 agaaatgagt actcaaaata ataataataa caaagaatgt aaacatacag ttctatctat
     3061 aaaagatagg tcttgcactg tttgtggaat gtcaagatat gatcaattac agaatacaga
     3121 aaagggatta accaaagcag aagaaaaaga attggaaggt tatataaatc gaaaacactg
     3181 ggaattgata gactgactat tataatgaaa atggcttcta ataataataa tcaaaatatt
     3241 aatccaagac cttgtgttta tggttgtggt attcagatat attggaatgc atcagtaaat
     3301 gaatactggg aagtattaac aaagaagaaa catatttgtc ctaaccgcgt caacaaatca
     3361 gtaactaata ctactaattc tccaactagt ggagtatcaa ataaaccacc atattccaat
     3421 aaaaaaccct tgtcccctac tccaaaacca aagatgtcca attcatttga atatttgcaa
     3481 ggatcaatag cagaaattca aaggaaatat gagattttat ctgatcttgt aactgaacat
     3541 aacggtaagg ttcagggttc tcagggatag agattcaaag actggcctta ctgatttgtt
     3601 ggtatattat gaagttcctt tgggacaaag ggaagaagta aaaggaagtt taatgatttt
     3661 gttttatcta ttataactcg aactaattag tagtcagttg actatattta tttatagttt
     3721 cgatatcctg ggttcttagc aatatcatca ttatcctcta taggataaaa tgtatgatta
     3781 taactccatt ctatgtctgt aggttcgttg catttatcaa caccaattcg tgtaattgca
     3841 atttttgctg gattaacaga agaaattctt aagaatctat ttctttctaa aaaatgtata
     3901 acaggctcaa aaacgctagc attgtctgga tttcccttta gatctacata tttttgaaat
     3961 ttctctggat ctgctttgaa accattcatt ggtgtttgtg caaaaacttg ttgttttaat
     4021 ataaatttcg caggattact ttcagacagt ctattcaaac aattaagaaa ccagggtgct
     4081 aaaattctgg tatcgtggtc ctcattttct acactaactt tgaaatccat ttaattacta
     4141 agcgagaatg tattattaaa aaagattgtg cctgggctat caaagtgctc ttatcaataa
     4201 ataatttgtt gtatttagaa tattaggata ggtaatttac aaaacagtgt atcaacaaaa
     4261 aaataataat gaaaataaga aatacaattg tgcatgtgga gtagcatttg taacattaaa
     4321 agaatattat aaacatcatc attactacca cccagaatat cattagtagt tgttagaaag
     4381 tcaaactctt ctacttctgc ttctaaatct tctttttctt cattgtatgc tttaacagac
     4441 ttagaataca gtttttttcc tttttctata gtggaaacat ataggaatgc ctaaagggtt
     4501 tttcgctcct actgtacctt taaatcctat agtaccacca cttaacttaa ttctattccc
     4561 attttttcaa gatgcagagg cttcaagtgg tttagacgag ataaaagaca tcacaaagag
     4621 tatgaagaga tcagttgaaa agatggaaga acaaggagaa tgtgcggaag agaatttaga
     4681 aaagacttta ttctatagta aaaaaggtaa aatatatcat ggaaatccgg attgcgatcc
     4741 gtctaaacag tatagatatg atcgattaaa caaaatgagt ggaatagacg aatataaaga
     4801 cgcacataat tttatattga gtaacaaaaa ttatttccaa gcaaaaaatg aggacgacga
     4861 aacatctaat aggacatata aaggatttaa tattattgct ttctattact gttagaatat
     4921 tatcaattat atctttaaga aatttacttt ttcttattgt tgctagaaga aggaattgct
     4981 atttctccta ctgctttatt atttaggtct ggtttcaatt ctattccttt aattatacct
     5041 gtttgttcat tatcatgaat gaccttattt tcaggttgac ttattagaaa tgcattaaat
     5101 ctatcactaa gaaatttaat agattcttcc aaacttttaa tctgttcttc cttctcctgc
     5161 agtttaccct tgataacata ttcagaatct tgattctttt cctttagttc tttaacttgt
     5221 ttagataatc tattttcgtc attgattgtg agatcatcta ctgctttgat atactcttca
     5281 agcagttctc gttcggaagg tctatagtaa ttttttgcta gacctgtatt atgaccgagc
     5341 atcatttcaa catgtaaaga tttcattaca tgttcggctt gggtcttgaa gaattttcga
     5401 aagccatgca tgcccttcca ttcccttcgt ctactccctt tcttcaaagg agaccataac
     5461 ccttgagcat gtattgctct ctctatcata cttttaactc catcacttga taaatgaact
     5521 ggctcattga tatcccccag ataattgcta tggtttggat cacgtttcca tgttctttct
     5581 gagacagtga aaaggttgcg cattacccat gaattgccag ttattcgttc accatgaaag
     5641 gcacgaaagt ccatccattc ctttaacgaa ttgtatgctt caggtgtaat aaaagaaaaa
     5701 tattcttcat tatctccagc atagacaata attttggctg ctattattat tgtattatca
     5761 tgattattaa ataggggaac aatatgtttc cattttaaat agtcccaagt ctcttttcta
     5821 atccctgatg ataacatggt taacaatata ggcttgattc ttttgtcagg ataatccatt
     5881 agtttcttaa tttcctctaa tgttggggct ctgtcatcag aagcctgttt ttgctttgga
     5941 agacccttac tcaatctctt ccaatttaat gtaatatcat tcatatcgca aaaaagtttt
     6001 gctgccttgt agtagttgcg tatagttgta ctttctatct ctcttttgac aaccctctct
     6061 ttttgaaatt caataaattt ctctatatat agaacagtcc acttttcatt aattgaagcc
     6121 ttttcataaa atattactgc ttgtttttgc aaatctagtt taggattgat accaaaatca
     6181 aagaattttt tgaggcgcgc tggccattgc cttcttgttt cgggtgcctt caatccatac
     6241 atgaacgaag aaaacggatc tttcttatcc tcttcttctt gatcttggaa aatcaataga
     6301 tcgtcatctt ctcttctacc tgggacgtcc aacacaatat aaatacaaag tgggcttata
     6361 ttgttatcga tgtgggccta aaggggtttg aaccctcgac ctaccggtta tgagccggtc
     6421 gctctaccag gctaagctat aggcccagct atttcaatta tattcaataa ataggttata
     6481 aaaatttaag tctgtaaaat tatgaatgac taagattaat tcatagtatc ttttacataa
     6541 ttattgtaca ctgtatcaag gatttgattt tgagagttca ttgaactaac tgatgcattt
     6601 aaaatatttt tgtttacatc tttggtattt cctactaatt tgttatcaag caaaattgtt
     6661 ctccataatt tggcgtgctc tatatcggca atttgatgta tcttaaaata ttttatatct
     6721 tcatctgagg aaatgttata aaatctcact aatccttcaa cctttgtttt actaatttga
     6781 ggtagttctt tctcaaatga atacatagct gatgcacctt cgcatatcga tgaaaaacac
     6841 aattcattta aattttcaat agctaagtta acctcattat ttccttcgta ctccaataat
     6901 tctttttttt caacgcctaa tgactgtgca aattttatcc atggttctat atgttgatat
     6961 tcttcactct gggtattttt taaaggtttc acatactcat taccaggatc ggagttgtga
     7021 agattcttat gaatgttatc aacaaattgt ggaacagctt tgaccaactg aaaatattct
     7081 tttgaataac tacgtaaatg gttcaaagtt aattttcctt ctttccatag agtataaaat
     7141 tgatgattta ataaactcct tttactgatt tcatcgtcaa ttattttaat taataatcct
     7201 acattaccag aatccttatg attgttttcc atacttatta aaatacagaa acattagaaa
     7261 aaccttttgt attttaattc ttgtggagtt tttgaattaa gaaaagattt tatagaaatt
     7321 aaaattacaa atcatcgtgc tccggtggtg tagtagctta cttatggctg cacaatccgg
     7381 tcaagcattg tggcctttcg agtcgcagat cccgggttca aatcccggcc ggagcacaga
     7441 aatatattgc ttaataacta ttagtctata attaataaga ataaggccat agtagcatta
     7501 atattctgat tatttttaac taaatggaag attaaatatt tggatggaga tactataaaa
     7561 ggaaaaaaca caaatcaaga atctagtagc tcattgatgg aacaatttgg tgatttttta
     7621 ttaaaaatta atcgtacttt agagagagaa ttggatctct attcctggtc tgaattttat
     7681 tcttcactta aatatgcctg cgatggtgga aagcgaattc gtcctcttat actattatta
     7741 tcatctgaaa taattaaatc taaaaacaat gataatactg acctcttaaa tactcctcaa
     7801 gctttttcaa aaagagatga cgaaaggttc ctagcggctt ctgcaatcga acttttacat
     7861 acagaatcag taatacacga tgacattatt gatgaagaaa atgtgagaag aggaaaacct
     7921 tcttttcata taaaatatgg atataataac agtatattaa cggcagattt tgtgcttgga
     7981 ataattctaa atattacatc gaaattaaac aatccacgaa tatcgtcaga gatagcgaat
     8041 gcagctattc gtatgagcga aggggaaata atggaaatta gactgaacaa agatcataag
     8101 attagtgaag acgattatat aaaggtctta gaacataaaa cagcctctct ctttgaatct
     8161 tctacgagaa ttggtgcaat tttagctcat gccgatgagc aggacacacg agcgatggga
     8221 aatttcggtc atttgatggg aatagcgtac caaattcacg atgaccttga tgattggaac
     8281 aatgaagata gattgtttaa cttattaatg aaacagaata atcattctca acaccttgtt
     8341 agtaaaatgg aaaacttgta caaatcttat tcactgaaag caaaaaatga gcttaattta
     8401 gtaataagta atattaaaaa taattatacg gagcgcttaa aacatcaatt agaagagcta
     8461 actaacctta atatgttacc atcttaatct tcttaagtat tattattatt tagtttagtg
     8521 ataatatttt ctgaaataat ttttatctac ttaccaaagt taaacagaaa attaataatt
     8581 tttctttaaa tacaagaaaa aggcaaaatg tttaacaatt taattattac ctccttatat
     8641 ttaatattgt aaatttatat tctcaattat ctactgtcga agattcaatt cttaatactt
     8701 gcaagtaata tattatctat atttattgct atgagaatga actttttgct attttttatt
     8761 tatagttatg tcttttaact ataaaaagta gttctattta ttctgaaaaa taataaccta
     8821 ttctagatat aaaataagga ttgctttcaa aagtaaaaag caattttggc ctcaagattt
     8881 gcaatgataa aaaaatatct ttttagctct tttcttctaa ttcctctgat tcttctcctt
     8941 cctctgtaat tttggtatct tcttgtagtt gttcagtgtt ttctgaacca gtttgatcgc
     9001 ttgtttgttg tgctaattct ccagtagcat aaccattccc aacttttgta attttaactt
     9061 ttattttttc gtcaaccttt gttcctggaa caaatattac aaacccttct atctttgcaa
     9121 ctccagaatc tcctctgttg ctaatagctt ctatggttac atcgtattgt tcgcctacct
     9181 ttactggtga atgacgaaaa cgtctaggtc gatttctatc tccatatcct ccaccactac
     9241 tatttcttct tcctccacta tatcctccac catatgacct gtcggaaaga tatgttcgaa
     9301 cttcagttgc tggtataaaa ctagctattc tcaattttgt catacattct atatttcctt
     9361 agatatttca actttagtat ttttaacttc aaaaaatgat gttaaatagg aaaggatatt
     9421 aatattagaa tctttatttt aatatcaaag aaattatttg aaagggaaaa cttgatttta
     9481 ttattaataa ttttattttt gataacgtaa aattgtaata ggtggatctg acttattaaa
     9541 aaaagacatg agctctacta tagcccattt aagataaaaa aagatggata aattcattgg
     9601 ttttaaagtg gtagacttcc actaaagacg ctaatataaa atagtggtaa ttgtataatt
     9661 gattttatta tttcttccat agccatacta tcttccgaat taaaaaagtc cgtagtatga
     9721 tatggcatat aataaagatc aagtacgata aaataaataa gatactttcg agaataattg
     9781 gagatatgct tcataaaata tgtgatgtca atacgataaa aaataatagc atgaaagtct
     9841 ttcatttagg actagatagt aaagtaatcg atattttgat tggtaaaatt aacgggaaat
     9901 tgtttgcttg tgataattat tgtccacatc ggggagcatc gttatcaaaa agtggcatat
     9961 catctgacaa ccagcgtatt atatgttatt tgcatgattt tgagtataat gtaaatacag
    10021 gaaagcttga aaatattccg agtaaatggt tgactcaaaa tccagaatgg aaaaaatcaa
    10081 atgacttggt tatttataaa ataattgaaa aagaaaaaaa tatctatgta gatattcctt
    10141 aagatattat attctatatt atataccaca attaaatttt ttacatctaa tatttcattt
    10201 ttgaaatctt gcagctgact caattacttt ctttttttca tcattaagtt catccagtgt
    10261 cataggaatt accaatatga tattatttga gttctcatct aatgtacact ctaaaggtcc
    10321 ttcgctaggg acatcttttt ttgtaaatat tgcagcatat gttgaatttt ttattaattc
    10381 atataaaggg cgattattag ccgaaaaggt taattcaatt tctccaagtg gaattatgct
    10441 tccatattca tcaatgtcga gttgggtaat tctaactata tttttagtat agctagtaat
    10501 ctttgcaagc atattaatct attataaaag taaattaata accttttatt agacagtttg
    10561 atactgatca ttttatctat aatttatctg tatgaatggt agatataatt gataaacaat
    10621 aatttttaaa aaatgtgata tatttttatt ctatgtgatc aaataatatt attcactagt
    10681 tattattgaa agaaatggta gcagaaaaag atgattttct tgtctgaaat aaatgttaat
    10741 cctactaatc acaataacaa ttcagaaatg tgatttttgt tttatcttcg agtccattgc
    10801 aaccattcct cctgtaaata caaatactcc taaaattgtt ataaaaagag acgcgtattc
    10861 ataagaatat acagatatac caagaatcgc tgtttttggg taaccgttag gtgtcaaatt
    10921 ctgattgact gtatcttgaa ttactataga tccactagtt atcattccaa tgaatcctat
    10981 tgctagaaga gatcctccca atattattat atttcttctt tgtttaccct gatggagtat
    11041 gaagaacgct atagcagaaa atatattttt caaaatcatc actataatta cagtcactat
    11101 ttcttatata acttttgata actgagtaaa tcgtccattc aatttatttt aataagttaa
    11161 tcagaatatt ccattttttg agaattatta agtcagaagt aacggaaata agttaagttt
    11221 gataacatta gttaaaaaat tagacctgat ataataatgt cttattgtca ttaaaagaaa
    11281 aacaaacaca agaatttgct cgtctgtcgc taatctgaga tgctcttgtg atggtcaggt
    11341 tggctctgat gatttttcag gaaaagcttc ttcggcaatt gtctcaattg gttcgcctgt
    11401 tggtaatgga gcattcctat ctactttata ctttgagagt tctgggaaat ttttcccaat
    11461 tattacattc attcctactg ctattatatc tgatttggga atatcatatc tatcattaga
    11521 atatccaaat actactattt tgtcatttgt ttcttttgcg acaaaaccta gatcgtcctc
    11581 attttctgcc ctaaccccct tattaaagag agatttaggg tactttcctt cataatatgc
    11641 taaatcaact tgtttttttt cgtctttcca tggatctggc cgactggtcg ggagtggatc
    11701 attgcgattt actttgtatt ttttttctac ctctgaaaaa ggaagaccaa taagtacatt
    11761 tgcgcctact tgttgaatct cagataatgg aatatcatat ctatttcctt ttccaccaaa
    11821 tactattatt ttatctggag tctctcttac aacgtaccct atatctggat tatctacagt
    11881 tttcactcct ctgttaaaat accttcccga taccataatt attatttact agcgttatat
    11941 aaaaaacatt actaaattat ctatgccaat tgtattttaa gagaaaatca ctgtaaagat
    12001 cttggcgatt tttaattaaa taattcttta tcacggaact ttcatacaca gtattaatct
    12061 ttcttataaa aagagttttt atgaatggaa ttgttttggg aaagaaatga gaaatattta
    12121 tgttaaaatc aaagcaatca taaatttact atagttataa tcattcgaaa tactttttgt
    12181 caataaatat tattatcaat attattatta tggtcagtct atattctcca gttatcatta
    12241 aatataattg tctctttttg ttataataga tcttttaatt agtatcatta gattagaatt
    12301 ataattttct atattattct attaaataac aatatttgta cattagttac ataatattaa
    12361 taaaattaaa tgtaacccta tattagttac gaatttaagt tatagtaaaa atgaaaaaaa
    12421 atagggatat aaaatataga tataggttag tcagtcttta agtaaatgat tcatcgagta
    12481 atcttaagta gcgaaccgga tattataaga tttaaattta tactaatatc caattatata
    12541 atggcatata aatattaatt cgttattagg agaagttttt tttttaaaat gataaaaaaa
    12601 attagatatt atatcgccaa aattataaag tataggattt tagttattcg ttatgtttcc
    12661 aaatgatgga gctaattgat caatgtgcga ggggacagat attgaggcga attctaataa
    12721 tggggcatgc catataccaa atccaaccat aaatattatt gcaaatatta atacgcctag
    12781 aatagcctta ggttctttta cccttgacat atcaccactt tcatcaaggt acattttttt
    12841 gataacccat aaatagtaac caagagataa agcactattt aacaatccag ctatcgctaa
    12901 gtacgggccc catgagatta cggttccact atcaattgca gattcaaaaa gtaccaactt
    12961 actccaaaaa ccattcaacg gaggaactcc agctaatgct aataatgata ttgataatgc
    13021 gatggccgtg atgggcattt ttctaccaag tccactgtac ttttctaaac tgtaactggc
    13081 taatgctatt gcaacagcag ctatagctaa aaatgatgct gatttcatca cggcatgatt
    13141 aaggatatga aaaagtgagc catccaaagc agaacttgcg tatggggcca atgccagtcc
    13201 aatcatcata taaccggctt gagctacact ggaataagcc atcatcctag gtacactttt
    13261 ttgtataaga gcaccaaaat ttcctactgt catagtaata actgctaaaa tagcaagaac
    13321 tatactccaa tcgagtccaa tggcgaacat tcctattact ataactctta tcgctgctgc
    13381 aaatcctgcc tttttagttc ctgccgccaa caaggcacct attgttgtag gagcaccttc
    13441 ataggcatcg ggtagccaca tatgaaaagg aaccaatccc attttaaacc caaagccagc
    13501 aacaaacaaa cctattgcaa gataagaaat aggaataaca cctccgagat tattattatc
    13561 tggtgtttgg tttggtactg aaagttcagc aagaattgtt atagaatcac caatatttgt
    13621 tgtacctgta actgcataga caaatcctat cgcaagcacg agaattgatg acgagaaggc
    13681 gccaaacata aaatatttta tagcagcttc atttgaaata ggatccttct tggaaaaagc
    13741 tgctagagcg tatgttggta ttgacataag ttcccatgca ataaatagca tgacaaaatc
    13801 ggtcgaatat gctatcaata tcattccaat cgttgagagt aaaattaagg aataatatga
    13861 agaatagttt gaaattcctt tcatataatt ccatgatgaa gccgagacca ttatggatac
    13921 gattaaaaga gaaaccgcaa agaaggaacc gaacatatca tctgcaatca catcgtttcc
    13981 aaaactcatc gcgggaagag gttctccact taatactcga gagattacta taagtaatgc
    14041 aataattaaa gtaccaaaag ttatcgcact aaaaagttga tttctacttt ttcctttttc
    14101 ttttttataa gcatctatta taggaattcc aagacctact gagccaagaa gtataattat
    14161 tataattggc gtagacgaaa tatcaatcat ttattatctc ctccattcct cctataagta
    14221 taacataatt agaacaacga taattcc
//
LOCUS       DASVEV010000279         7637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1317130, whole genome shotgun sequence.
ACCESSION   DASVEV010000279 DASVEV010000000
VERSION     DASVEV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317130"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..595)
                     /locus_tag="VF222_12250"
     CDS             complement(248..595)
                     /locus_tag="VF222_12250"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX7143237.1"
                     /translation="MKDLDNNKTIKNEDVYDLLRKIPAGKVTTYGDLAKALGNPSASR
                     IIGSIIGKNPNPIQVPCHRVVMSDGKLGGYAYGTAKKKELLEKEGLSFINGIVSDFKK
                     VRVYISSKEQDRG"
     gene            690..1421
                     /locus_tag="VF222_12255"
     CDS             690..1421
                     /locus_tag="VF222_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143238.1"
                     /translation="MTHTLIIRGFNDQVHEQLGEIANERGVSINSIVKDAVDMWLKKN
                     ESEVPRKHHLVIYSDDKSIMRLLKSMDRLAKEGNLFRCFFGPPHNPSTELLSKLKWYD
                     GTAEPYYYSSPTLQKSIEKEKLQIQSKAQVQNQKNLINYCNKVIENVIKNAYNKPVCC
                     MDFLINDVGKDSLKQALSIEKVYDDNRIPGLMYCTYKTDNLLNSQIKDLIELFEMHDQ
                     IFILKEDEVYKLHLTKENVHKLFLS"
     gene            2632..2763
                     /locus_tag="VF222_12260"
     CDS             2632..2763
                     /locus_tag="VF222_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143239.1"
                     /translation="MLPISVAATVDNIINEAIKTSPNIVTDYAYYGNNEQIYLKSEK"
     gene            complement(3025..3219)
                     /locus_tag="VF222_12265"
     CDS             complement(3025..3219)
                     /locus_tag="VF222_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143240.1"
                     /translation="MGYKGGHSLVISPPELEFYFFKVSELLDKGKVSYKTESSIGKQY
                     GQVFLDNTKHWNSQNLKKLN"
     gene            complement(3532..4005)
                     /locus_tag="VF222_12270"
     CDS             complement(3532..4005)
                     /locus_tag="VF222_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX7143241.1"
                     /translation="MAYATFDMKNYPLILLFISLVIGISFLPSVYGQDDVSKTIAKKN
                     ANPIVYHLTSNDPWRASIAISDATNLNNLGYNITLLLSIEGVQVGVKNPHHFLNLNDV
                     VANVTNFIMDGGKVVVCGVCLRVAGFQPNEIINGAIIGNDEITSKIFTNATVVTY"
     gene            complement(4437..6206)
                     /locus_tag="VF222_12275"
     CDS             complement(4437..6206)
                     /locus_tag="VF222_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143242.1"
                     /translation="MSYKIIDSLHQDIGKTIASERFLNTMDGFWFALKPNVIINSFDF
                     VTVDNLFNSKSIGIIKELQAVSAEHDYYNNYLSDNHTISSTKKQLKQKGKEKEDEEEE
                     NEQLSNRQLNNLILVKVAIMANTGIKVQGYKKNISINFPVGVGKTVRFATEDEIIFAL
                     GIPQMENPIPAGIIETTNGLEVPITLDVTYLAGADTAHVNASGISGNRKSSYILFLLQ
                     SSYQTLKRMNQNVGLIIFNTKEQDLLYIDQKEKYTKKRTERLFDVLDLDIEPFENVTY
                     FLPRGKDGKPNSIHIPKNYRTYSYELKDVYDRLELLFSEPYDLHHDFLSIMNYIYESW
                     PLKSNIGKKDISTWTDLSEFKEYPETIVSNKSSLLYFLGRLQRFRKSPMFIDKKKTST
                     YLGNEIKKIKAGEVFVIDVATISSYEEQAFVVGDVMKSIDEMYSSRYYYLEDDNRSNN
                     INIDSNKNNVQNDQKKLHYILVFVDEINRFIPKSQNGKMNSVSEQIMRTVTAGRTRGT
                     ILFSAQQFKSATDYRLHENTDLHITAKLGLSELSTEPYSMLDESTKMNIVRLNKGELV
                     MVHSAFRHPIKIGFPKATYKEPY"
     gene            complement(6515..6700)
                     /locus_tag="VF222_12280"
     CDS             complement(6515..6700)
                     /locus_tag="VF222_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143243.1"
                     /translation="MSTFKRKLGYALGIFFILTSLYGLAIILYSPSSTQVFEMIVLTS
                     SILAIGIIFIYLARRIR"
     gene            complement(6861..7094)
                     /locus_tag="VF222_12285"
     CDS             complement(6861..7094)
                     /locus_tag="VF222_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143244.1"
                     /translation="MAENLFNKFNEYIRDVQNDEIKSIVIGLFALIGFIILIYSIFIG
                     IVGEPLASVTNTVFVAVIIGSFTLVGIIIGKRL"
BASE COUNT         2654 a         1105 c         1053 g         2825 t
ORIGIN      
        1 aatggctttt gattgtgctc ttggaatttg ctgagtcaat acaaattctc ctccagtata
       61 gtcatcatca ggctaagtct tgtgatgaga tcagagctgt catattcact gataagttct
      121 ttagaatatt gacctgacaa gaatcgaggt accagggcat atcctttttc atgcatattt
      181 cttgttaccg tttcttagtc aataaatgaa atctttttta tcataacctt acttgtattc
      241 aatgttttta acctctgtct tgttcttttg aagatatata gactctaacc ttcttaaaat
      301 cgctaacgat tccattgata aaggacaaac cttctttttc gagtaattct ttctttttag
      361 cagtcccata agcgtaccct cctaatttgc catcagacat aactaccctg tgacaaggta
      421 cttgaattgg gtttggattc ttgcctatta tacttcctat gattcttgat gccgaaggat
      481 taccaagtgc ttttgcaaga tctccataag tagtaacttt tcctgctggt atttttcgca
      541 acaggtcata aacatcttca ttttttatcg tcttattatt atccagatct ttcataatat
      601 aagatatcat gatagcgata tttatatatt tattcatatt ttgctatctt gatatcaata
      661 tttatatata ctcatgaaaa atatatttta tgacacatac tctcattatt agaggattta
      721 atgatcaagt tcatgagcaa ttaggagaga tagcgaatga aagaggagtc tctataaatt
      781 caatagtaaa agatgcggta gatatgtggt taaaaaagaa tgaatcagaa gtacctcgca
      841 aacatcatct ggtaatttat tcagacgata aatctattat gcgactgtta aaatccatgg
      901 ataggcttgc caaagaaggt aacttgttta gatgcttctt tgggccacca cataatcctt
      961 ccactgaact tctgtctaaa cttaagtggt atgatggcac ggctgagcca tattactatt
     1021 cttctcctac tctacaaaaa tctatagaga aagagaaact acaaatacaa tccaaagccc
     1081 aagtccaaaa tcagaaaaat ctcattaact attgtaacaa agtaatagaa aacgtaatta
     1141 aaaatgctta taacaaacct gtatgctgta tggattttct aattaacgat gtaggaaaag
     1201 attcactaaa gcaggcactg tctatagaga aagtttatga tgataataga ataccaggat
     1261 tgatgtactg tacttacaag actgacaact tgttaaattc gcaaataaaa gacctgatag
     1321 aactgttcga aatgcatgat caaatcttta tactaaaaga agatgaagta tacaagctcc
     1381 atttaactaa agaaaatgtt cacaagttat ttctgagcta atggtatata agcggtatag
     1441 cattgttagt agtaagaaat acgatgccga taacaaacgc agcacttatt gaagttataa
     1501 tttcttttgt tttcataagc atctggcaaa aaaaaagata tcgaattatc taaatttgat
     1561 ggtcatcaaa aaacaactta atttatagtt ctttgaatag tatagtatta acaagtttga
     1621 tttgaatgtt tatagctata acaatttcgg ctataagaaa ttacttcttt atgttgtaat
     1681 tctaaataaa ttactattta ataaaaaata tcaacaaaat taaatttttc ggcgcctaaa
     1741 aattttcaat aagataaata aatttaataa ttaaaaggat aattggaagt actcagattg
     1801 tatcctattt ccattacaca gctattgtgt aatgatatta attagatggt attggtatga
     1861 tcaaaggtat ttgatattgt tatttataca atttattttg attatactac ttttatctgc
     1921 agatttgcaa tagctgttgt ctttaccatt tgatagatct tttaactcgt aaatagcatt
     1981 tccatatgat ttcttgtcgt cgtgtccgat tcatattctt ctaattcggc ttgtgtacta
     2041 ctgaccataa ataatggaaa gatagagcaa ttattcctgc gactagtaca attgctaaaa
     2101 aatattgctc tattttggtg taatttgttg ttttttaacg ttatctaaag atatttatat
     2161 acatagtatc taaaggaata ctgcaattat tcctgctagt ataaaaatta cacatttttg
     2221 tgtaattagt tgttgttcaa cggtggatag ccaataagtg gttacccaat tgcgataact
     2281 cggtgggatt taatcaccgg ggaggttgca aacctattcc tcaactatta tataaaataa
     2341 agtctattta agtttttttg caaaagttct aaattcaaga ataaaatatt atttaacata
     2401 tataatggat attacctaat gactatttca agatctaaac ttaaatgact aataatgtaa
     2461 aatatattat aattatgacc ttgataacca agcttaaata tgtgcaatta caataatatc
     2521 tataatttta gattggttaa aacaattttc agataactta ttattgtaat aacattttga
     2581 cattttgttt tattgttaat tattgaaaag gaattaaact tgaaattatt catgttaccc
     2641 attagcgttg ctgctactgt tgataacatt atcaatgaag ccataaagac aagtcctaac
     2701 atagttactg attatgcata ttatggtaac aatgaacaaa tatatctgaa gtccgaaaaa
     2761 taatagagta acatacgagt tatatggatg tagaaggatt attgtttctt tcaatccatt
     2821 atctttttat tcaataaaat gccgaaaagt gtttctaatg ttgatttgta cttttattaa
     2881 tctaatatag tatttgttta tcatcttctt atattatttt gaacaatttt aattaagtga
     2941 acattaactc tatcttttct gaccaacaag caacgcataa atatcgatat aattatcaaa
     3001 tatgtaacaa actttacaga tttattagtt taatttcttc agattttgac tattccagtg
     3061 ttttgtattg tctaagaaca cctgaccata ttgttttcca atacttgatt ctgttttata
     3121 tgaaactttg cctttgtcaa gtaattcact tactttaaaa aaataaaact caagttctgg
     3181 aggactgatt acaagagaat gtccaccttt gtaccccaca tcaaatctat gtagagatcc
     3241 tttgggaaca aaaattatat cttttaggtt tacccgttac tgaattccct tctaaaatga
     3301 atttataatc gccttcaact atgtaaaaag tttctgaacc attaggatgt atatgaaatg
     3361 caagacctat atgaggtgga tgaattacat ctaggatagt atagataccg ttcgtttcag
     3421 aagtaagaac cttgtagtaa attttgcacc atgaaaatca aatggtttat tcttcacaaa
     3481 aaatatttta tggtttaaaa tatatctgtt tgatttcaca aataggttaa atcaataggt
     3541 aactactgta gcattggtaa atattttaga tgttatttca tcatttccaa ttattgctcc
     3601 attaattatt tcattgggtt gaaatccagc tactcttaaa caaacaccac acactacaac
     3661 tttacctcca tccataataa aattagtaac atttgctact acgtcattaa ggttcaaaaa
     3721 atgatgagga tttttgacac caacttgtac tccttctatg ctaagaagaa gagtaatatt
     3781 atatcctaaa ttatttaggt tagttgcatc agatatagct atagaggccc tccaagggtc
     3841 attgctggtt aaatgatata caattggatt agcatttttt ttagcaatgg tttttgaaac
     3901 atcatcttgt ccataaacac ttggtagaaa agatatccct ataactagac tgataaataa
     3961 caatatcaga ggatagtttt tcatatcaaa agtagcatac gccatatttt attttaatat
     4021 ttcttaaata tataaaataa attctttgaa aaagtaataa aatataaagt ttgttatacc
     4081 aactcataaa taaatataat ttttaaagtg ttttgaaatt tattgaatag gaattcgtta
     4141 gcaatttaca ggagtgagaa atatagatag gggattctaa tataaaaatc taatgtagag
     4201 atcatagtat cttgtattag cttttattat tagaaactta ttggaaaaag atattgatac
     4261 ttataaatat cgtaaatttc cagtattgaa accacttgtt gagcagttgt cttagtgcta
     4321 acgatactag gaattccttg ttggacgtgt gctttgttag ttaatagaat tcggcatatt
     4381 caacggaaac ctccaaattg tcctgtttta caggcagtct tcttatcttt gttgaattag
     4441 tatggttctt tatacgtagc cttaggaaat ccaatcttta ttgggtgtct gaatgccgag
     4501 tgaaccataa ccaattctcc tttatttagt ctaactatat tcatttttgt actttcatca
     4561 agcatagaat aaggctcagt agataattct gataatccta gttttgctgt aatatgtaaa
     4621 tcagtatttt catgtaatct ataatctgtg gcacttttaa attgctgtgc agaaaataga
     4681 atagttcctc ttgttcttcc tgccgttact gttctcatta tttgttctga aactgaattc
     4741 atttttccat tttgagattt aggtataaat ctattaattt catcaacaaa aactaaaata
     4801 taatgtaact ttttttggtc attttgaaca ttattcttat tgctatctat attaatatta
     4861 ttgcttctat tatcatcttc aaggtaatag tatcttgaag aatacatttc atcaatactt
     4921 ttcattacgt ctccaacaac aaatgcttgc tcttcataag aagatattgt tgccacatct
     4981 attacaaaga cttcacctgc tttaattttt ttaatttcat taccaagata ggtactggtt
     5041 ttctttttat caataaacat aggcgatttt ctaaatcttt gtaatctgcc taaaaagtac
     5101 aaaagagaag atttgtttga tactattgtt tcaggatatt ctttaaattc agaaaggtca
     5161 gtccatgtac tgatatcctt ctttccaata ttacttttta gaggccatga ttcataaatg
     5221 taattcatta ttgataagaa atcatgatgt aaatcataag gttcggaaaa tagcagttca
     5281 agtctatcat atacatcttt tagctcataa gaatatgttc tgtaattctt tggtatatga
     5341 atagaattag gtttgccatc ttttcctctt ggaaggaaat aagttacatt ttcaaatggt
     5401 tctatatcta aatctagtac atcaaataat ctttcagttc tttttttggt atatttttct
     5461 ttttggtcta tatatagcaa atcttgctct ttagtgttaa atattatcaa tccaacattt
     5521 tgattcattc ttttaagtgt ctgatatgat gattgcaata agaataaaat ataggagctt
     5581 tttctgtttc ctgatattcc tgatgcatta acatgtgcgg tatctgctcc cgccaaataa
     5641 gttacatcca atgtaatagg aacttcaaga ccatttgttg tttctatgat accggctggt
     5701 atgggatttt ccatctgagg aattcctaaa gcaaatataa tctcatcttc agtagcaaat
     5761 ctaaccgttt tacctactcc aacaggaaaa tttatagata tattcttttt atatccttgt
     5821 actttgattc ctgtatttgc cattattgcc acttttacca atataagatt atttaattgc
     5881 ctgttagata gttgttcatt ttcttcttct tcatcttctt tttcttttcc tttttgcttc
     5941 aattgttttt ttgtgcttga tattgtatga ttatcagata aataattatt ataataatcg
     6001 tgctcagcac taactgcttg taattctttt ataattccaa tagacttact attaaacaaa
     6061 ttatctactg taacaaagtc aaaagagttt atgattacgt taggttttag agcaaaccaa
     6121 aaaccatcca ttgtattgag aaacctttca gaagcaatag tttttccaat atcctgatgc
     6181 aaagagtcta ttatcttgta tgacattaat tatgaatatg ctataaaaga taagtctctt
     6241 tgataatata gatactttaa actatagagt tcatatagca ttggtcttca gtatgtttga
     6301 acttgccatg tatggtaatc acttactttg tatttagctt ttattattag aaacttaaat
     6361 gaataaaaag aaatgaaatt ataataaaaa atttactatc atattttaac aaatccaaac
     6421 caagtaattc ttaaaataat aattaatgaa gatgaagaaa gttatttctg gattaattac
     6481 aagtgaaaaa ttttataact actatcaaat agaattatcg tattctgcga gctaaataaa
     6541 tgaaaattat tcctattgct aaaatggatg aggtcaatac aatcatttca aaaacttggg
     6601 tggaagatgg agagtataga ataattgcca agccgtataa acttgttaag ataaaaaaaa
     6661 ttccaagtgc ataccctaat tttcttttaa atgttgacaa ttctctatgt tatctatttt
     6721 atatatgaag tttatttcaa ccttattact cttggttcgg tggaatgctg tagaaatttc
     6781 aatacaattt cttttataaa ttgggattat gaaattgcaa tttacctttt acacttccaa
     6841 agatagaaat cttattcgtt ttacaatctc ttaccaataa ttattcctac caaagtaaaa
     6901 gacccaataa ttacagcaac aaaaacagta tttgttactg atgctaatgg ctctcctacg
     6961 attccaataa atatagaata aataagaata ataaatccaa tcagcgcaaa tagtcctata
     7021 actatagatt taatttcatc attttgaaca tctcttatat attcattaaa cttgttaaat
     7081 aaattttcag ccattaccac tataccaaat gtaataatat aaaaacaatt taaggtatta
     7141 ttgtaaaagg tagaattaca aagctcgtac aaataataag ggttgaaaaa ataaacggaa
     7201 cgttaaggaa atttagtcat ctaaactatt tagaaaaata aaacctaatt tctgcaagaa
     7261 atgagttcag aaacagaaaa ctacttacaa tttattcgat aactgaaaaa ggattaatga
     7321 atttactaat tgagaaaaaa attatttgaa ttttaaaata attttaatag atttattttg
     7381 atagagtatg atgaactgct tttgtcacta aaaatataac aaatcctaat gaaactgatg
     7441 taataatata aggtattatt gaggggattt tcacttaata agatagatag caattaaaaa
     7501 tataattgaa gtgcttaggt acacttgcta acatagtatt actaaattag attagttaat
     7561 gtttattatg atttccaatt gctaattttt attttaaaat caattaggaa aaatgattag
     7621 ttgaattttt tgttact
//
LOCUS       DASVEV010000280        11168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1122506, whole genome shotgun sequence.
ACCESSION   DASVEV010000280 DASVEV010000000
VERSION     DASVEV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122506"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VF222_12290"
     CDS             <1..217
                     /locus_tag="VF222_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143245.1"
                     /translation="YFDKLINPSIHGSTMIEKFFNEHRDLRLYQNRLKDKGQEYLNSH
                     KSDFIKLMIEIDTKVRGTHLSNNIIDN"
     gene            291..1091
                     /locus_tag="VF222_12295"
     CDS             291..1091
                     /locus_tag="VF222_12295"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEX7143246.1"
                     /translation="MPKIFADVSEKEITRSIAGMFMETMNEYTDSDVIIIGAGPAGLS
                     AGRELGLLGIRTLIIEQNNYIGGGYWVGGYMMNPVTVRAPAQKIWDELGVPYRVIRDG
                     LYATWGPHACSKLIAATCDAGVRFLQLTKFDDLVIKNKRVGGVVVNWMPVSALPRNIT
                     CVDPIAFESKIVIDASGHDSVAVKRLMERGYVKWKGMDPMWVEGGEDAVVNHTGEVFP
                     GLIATGMSVTETHGLPRMGPTFGSMLLSGKKAAEIAANKLKELIEIPN"
     gene            1106..1909
                     /locus_tag="VF222_12300"
     CDS             1106..1909
                     /locus_tag="VF222_12300"
                     /inference="COORDINATES: protein motif:HMM:NF042804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HEX7143247.1"
                     /translation="MIFCKNINFFNDSSWPEIKIKNIIDHLKKIIPNNSIKNKDFLLL
                     NRYQKEPLFYQQSLIHYLKEPSWIYDLKGTRKEIDKDLDFKKKVPPDLYLYDGFLLQK
                     AFQLYIKNDELLSLSHLNILIIDKLLCTFDETDWKHHARSVICGNPTLISTSGIVEGI
                     AKPREYYYKLYSCDENPDLIREVKKEYEEKFIDYYDIRINDVIEGLVIQSLFYFINSG
                     SPFCENSYCRLFNAHFQDDLIIAHINHKLLCAPHQTILNNYNDLLMNYY"
     gene            1977..2942
                     /gene="trxB"
                     /locus_tag="VF222_12305"
     CDS             1977..2942
                     /gene="trxB"
                     /locus_tag="VF222_12305"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7143248.1"
                     /translation="MRLDEDNIDTTNQGLDIFDVIIIGSGPAGYTAATYTSRAKLKTL
                     IISGSLPGGQLMTTSEVENYPGFPNGIFGPELMMNMRQQSERFGSFIVDDEVLQVNFK
                     VNPFFVKTHSKRYLSKSILVCTGATPRKLGIAGEEAYGGRGVSYCATCDGPFFKGEDI
                     VVVGGGDTALEEATFLTKFGKSVKILHRRDSLKGSKILQEKSFANPKITFLWNSIITN
                     ISGNKKISKILIKDVNTQQERELNVGGLFIAIGHEPNTKIFQGQLELNEHGYILLKEN
                     TRTSVDGVFAAGDVHDFRYRQAVTAAGYGCMAALDLEKWLNENKN"
     gene            complement(3187..4185)
                     /locus_tag="VF222_12310"
     CDS             complement(3187..4185)
                     /locus_tag="VF222_12310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX7143249.1"
                     /translation="MTLTITESKVTEKIGNTPLIELSSFSTENVKLFAKLEWYNPFGS
                     VKDRAAYWMIKDAERKRVIKKDKSIIIEPTSGNTGIALTGISSSMGYKVEIVIPEKVS
                     AETKSILKNLGATIHETSDDLCPRVGAGTDQSIALAKAISTPRPDIYYMPNQYENDAN
                     YLAHYEGTGPELWEQTNGKLTHFLTGCGTGGTITGTGFYLKEKNPNIKVVAVQAQKNH
                     LLQGLRNFEESSMPDLFKRREIVVDEWYTATNKDSFAMVKELTKKEKILVGPSSGSVM
                     SAMLEARDKIESGIIVGIFADDGRKFKSLYQEQNVLNEEEYEKALQNAKFLSKLFY"
     gene            complement(4186..4428)
                     /locus_tag="VF222_12315"
     CDS             complement(4186..4428)
                     /locus_tag="VF222_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7143250.1"
                     /translation="MRDDIDFRKVIDVRGLYCPEPVFRTKIEIEKMTIGEKLKVVADD
                     PQSEEDISMWVNRNGHELISIGKKEADLEFIIKKAK"
     gene            complement(4496..5254)
                     /gene="cofE"
                     /locus_tag="VF222_12320"
     CDS             complement(4496..5254)
                     /gene="cofE"
                     /locus_tag="VF222_12320"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX7143251.1"
                     /translation="MLKKIEIIPIYINININPKLNLLDILLESIKNNNQIIKNGDIIV
                     IAQKIISKNEGRSIYLNNIIPSSRSIELGKKINKDPRIVELILQESRKIVRVFNNIII
                     TETLHGFICANAGIDQSNVSKSKNRVLLLPKDPDKSADSIRKEIYEKTRKNIAVLISD
                     TFGRPFRIGQTNVAIGIAGINPLKNYIGKRDMFGKIMKVTEIAIVDEIAGAAELVMGK
                     SEGIPVAIVRNAIYSKHHSSIKKIVRKESRDIFR"
     gene            complement(5238..5921)
                     /gene="serB"
                     /locus_tag="VF222_12325"
     CDS             complement(5238..5921)
                     /gene="serB"
                     /locus_tag="VF222_12325"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX7143252.1"
                     /translation="MLIIFDVEGVLLNAEYLPILAQVFGPSKEKEIWDITKQGIRGDI
                     DWEEGLRKRVKALKGIKFDDAKQIGENLEIMPGAKDLCSALKDAGWKMIAVSGGFTII
                     TDRLRKELGLDLIFSNNLIFKNGILDDLDLQVTSDKSLVVRPIIKKWGFKKEEIVVVV
                     DGANDIKLFDIAGYTIGFCPVELVKNKADDVVELRNLSLLKGLLEKRFGNWILKTNLN
                     KINSNVKEN"
     gene            6173..7279
                     /locus_tag="VF222_12330"
     CDS             6173..7279
                     /locus_tag="VF222_12330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143253.1"
                     /translation="MINSLRKSPRLEKIKNINKTFPMVSIIVPARNEERYIRKCVDSL
                     VKQDYPDFEIILVNDESSDKTLEIMNEYQNSYSTIKVLNVNRPDDDWTGKNWACYQGY
                     LKSKGDLLLFTDADSYHSENTMSLVVQNTKHYKLDAITVMPRLLCNDFFTKVTLPLLT
                     IFMHTHFSPLKVNDAKSKLGYFFGSYFLIKRDTYEKVGSHASVKHEIIEDSALGKKVK
                     EGNFKMRMVRGESYVETIWARNASELSKAIDRLAIPMFMENRLKLSLLSIVLFFLLIF
                     PFLIVSLLLTLSIIHPYILNFVILLLAGMTLVILFLANLIQITMTLFLNKFYSLCLLI
                     GATIIVSRFIFRLITSNRISIVNWRDRIYKITPN"
     gene            complement(7288..7947)
                     /locus_tag="VF222_12335"
     CDS             complement(7288..7947)
                     /locus_tag="VF222_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7143254.1"
                     /translation="MNPSILNYNFFSMDTNLVAKNLIGKKLVKIIRNQNGKIKLSGII
                     VETEAYGYKSDEASHAYRGITKRNMVMFGPAGRSYVYFTYGNHYCFNVTAKSINQVAG
                     AVLIRGLEPKEGIQYMRNYRNVDDKILTNGPGKITQAFCIGKQHNDQDLTDINNDLYI
                     EKGFLPNLVLATKRIGIKKSIHEYWRYILAYKNENVIFLNNFVSKKKENIHFQICRRL
                     L"
     gene            8040..9263
                     /locus_tag="VF222_12340"
     CDS             8040..9263
                     /locus_tag="VF222_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7143255.1"
                     /translation="MQKNLGINYELKLPLILIHTPFGLRFFDVIANSSVGKIYAAFNI
                     YLMPLITALAVFLILGSLLVLFSNDSAREGIREIGPQGNLLIPGLNPILPWTYGWIGL
                     VITIIIHEAGHGIVARVYNTKVESTGIVLFLGIPIGAFVNIQQDELNKTTFKQKSAIL
                     TAGPLNNMVIAGISFGLLFVLITSLNPISNIQGDETGITVVTIGENSLAKSIGLSKNS
                     IITFINAEPIENVQELRKILRDNLGTTIQMKWISEDKKEQIKSFELPASIPENKGVLG
                     VTLTDAFFDPGLVLDNYKNLFLTNPLAILMPPTLQQNLVPYSDSMSSYYTSTIFGPTF
                     PIIANFLFWIWFINFNVGIFNALPITMLDGGQWYGTLLENKIKTNSKIKPFTILSLVF
                     VIIVILSFALPWVLR"
     gene            9436..>11168
                     /locus_tag="VF222_12345"
     CDS             9436..>11168
                     /locus_tag="VF222_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HEX7143256.1"
                     /translation="MKKNNGNGSDKNNYNKSLNSETKSDSLDKNFKNQRYVKDEEAII
                     KSIFDSIPKESQITIARFEGPNIALYTKNPRFSLTELTYYLSSLSKTLKKRFIVRTDP
                     SIRLQEDETRTIVSKILPKDVIVSAVFCDDATGEVVLEVNKPESVISETIINIAESTG
                     WIAHLRRSPHIPSMSIKNIHTVLKISSKERSNFLRELGKRVFRDPLVKKGDESLESEN
                     PLERPSGNDKQSKTWNNKEVYIFCLGGVKQVGRSCFIVVTSESKIMLDCGINPGENNA
                     IDAYPRIDWFDFKLEDLDAIVISHAHIDHQGFLPTLFKYGYDGPVYCTEPTLPLMNLL
                     QSDSVKIAQNNGVYCPYEIRDINEIIKHCITLPYGKPTDISPDITITLNNAGHIMGSA
                     TVHVNISGAHNILYSGDYKYAKTQLLDSAVSNYPRVETLITESTYGASCDLMPDQSVV
                     YKSFTETINKTLTSGGKVLIPVPAVGRAQEIMLVLDKEMKDGRLIEAPIYIEGMISEA
                     SAIHMSYAHYLGQDVRRSVSQGINPFQSEYFTVVNGWGKRDEVLKDENPAIIMATSGM
                     LEGGPSVEYFK"
BASE COUNT         3718 a         1741 c         1763 g         3946 t
ORIGIN      
        1 ctattttgat aaattgataa atccttctat tcatggaagt acaatgatcg aaaaattctt
       61 taatgagcat agagatttaa gactctatca aaatcgcctt aaagacaaag gacaagaata
      121 tttgaattct cataaatccg attttattaa gttaatgata gaaattgaca ctaaagttag
      181 aggaactcat ttatcaaata atataataga taattaaaat tacttttgct tatatgactt
      241 tgcatatcaa atgcattaat aatatataat gtaattctcg tgataatagt atgccaaaga
      301 tttttgctga tgtcagcgag aaagagatta ctcgttccat tgctggaatg tttatggaaa
      361 caatgaatga atatacagac tccgatgtta ttattattgg tgctggtcct gctggtttat
      421 ctgcaggtag agaattgggt ctattaggaa ttagaacctt aattatagaa caaaataact
      481 atattggtgg aggatattgg gtaggaggtt atatgatgaa tcccgtaact gtaagagcac
      541 cagcccaaaa aatctgggat gagttaggag ttccatatag agttatacga gacgggctat
      601 atgctacatg gggtcctcat gcttgttcaa agttaatagc cgcaacatgc gatgcaggtg
      661 ttcgattttt gcaattaact aaattcgatg atttagtaat aaaaaataag cgcgttggag
      721 gggtggtagt taactggatg ccagtttctg cattaccaag aaatattaca tgtgtcgatc
      781 caattgcatt cgaaagcaag atcgttattg atgcgtcagg tcatgattct gttgcagtaa
      841 agagattgat ggaacgcggt tatgtaaagt ggaaagggat ggatcctatg tgggtagagg
      901 gaggcgaaga tgcggtagtt aatcatactg gggaagtatt tcctggtcta attgcaacag
      961 gaatgtcagt aactgaaact catggacttc caagaatggg tcctacattt ggttctatgc
     1021 ttctatccgg taaaaaagct gcagaaatag ctgcaaataa attaaaagaa ctaatagaaa
     1081 tcccaaatta ggcatttttt tttgcatgat attttgtaaa aacataaatt tttttaatga
     1141 ttcttcttgg cccgagataa agattaaaaa catcattgat catttgaaaa agattatacc
     1201 aaataactca attaaaaata aagatttttt attattgaat cgataccaaa aagaaccatt
     1261 gttttatcaa caaagcctta tacattatct taaagaaccg tcttggattt atgatttaaa
     1321 aggtactcgt aaagagatag acaaagattt agattttaaa aaaaaagtcc cacctgattt
     1381 atacttatat gatggttttc ttttacaaaa ggcatttcaa ctttatatta agaatgatga
     1441 actattatct ttatctcacc ttaatattct gataattgat aaattattgt gcacatttga
     1501 tgagacagat tggaaacatc atgcaaggtc agttatttgt ggtaatccta ctttaatttc
     1561 tacttctgga attgtagaag gaatcgccaa acctagagaa tattattaca aattatactc
     1621 gtgtgatgaa aatcctgatt taattaggga ggtaaaaaaa gaatatgaag aaaaatttat
     1681 agattattat gacattcgaa ttaatgatgt aattgaaggt ctagttattc aatcactatt
     1741 ttatttcatt aattcgggct ctccgttttg tgaaaatagt tattgtaggt tatttaatgc
     1801 tcatttccaa gacgatctta taatagcgca tataaatcat aaactcctat gtgctcctca
     1861 ccaaactata ttaaataatt acaatgatct tttaatgaac tattattaat gaatatatta
     1921 agggaacttt tgtgacaaat aattaattag tccctatttt atctattacg tacataatga
     1981 gacttgatga agataacatt gatactacta atcaaggttt agatattttt gatgttatta
     2041 ttataggttc agggccagct ggctatacgg cggcaaccta tacttctcga gcgaaactaa
     2101 agacattaat aatatctgga agtttgcctg gaggacaatt aatgaccact agtgaagtag
     2161 agaattatcc tggttttcca aatggaatat ttggacccga attgatgatg aatatgaggc
     2221 aacaatctga aagattcggt tcttttattg ttgatgatga agtactacaa gttaatttta
     2281 aggtaaatcc tttttttgta aaaacacatt caaaaagata tctctctaag tcaattcttg
     2341 tctgtactgg agcaactcct agaaaactag gtattgcggg agaagaagca tatggaggaa
     2401 ggggagtatc ttattgtgct acttgtgacg gtcctttttt taaaggggaa gatattgttg
     2461 tggtcggagg gggcgatact gccctcgaag aggccacatt tttaacaaaa tttggaaaat
     2521 cagtcaagat attacataga cgcgactcac tcaaaggcag taaaatattg caagaaaaat
     2581 cattcgctaa tcccaaaatc acctttcttt ggaacagtat cattaccaat ataagcggaa
     2641 ataaaaaaat ttcaaaaatt ttaattaaag atgtaaacac tcagcaagaa agagaattaa
     2701 atgtaggagg attattcatt gcgattggac atgagcctaa tactaaaatt tttcaaggtc
     2761 aactagaact gaatgagcat ggttatattc tccttaaaga gaatacaaga accagtgttg
     2821 atggggtttt tgcagctgga gatgtccatg attttcgtta tagacaggct gttactgctg
     2881 caggatatgg atgtatggct gcacttgatc ttgaaaagtg gttaaatgaa aataaaaatt
     2941 gatatgttat tgtataaata ataatttaag atgcaattgt tttatttaat tcaccgttta
     3001 agtcaaaatt taagttatct atcatttctc aaattgtacc taattttatt cgtcaattag
     3061 atttattccc aggaaattta tcacttagtc gatgacgaaa gttaaagata tcatctcata
     3121 ttcttattaa gttcagtgtg acacattata ttgtctataa taattttttc tcaaaaataa
     3181 tttcctttag taaaaaagct tagataaaaa tttagcattt tgaagagctt tttcatattc
     3241 ttcttcatta agaacattct gttcttggta taaactttta aatttcctac cgtcatcagc
     3301 aaaaatccca actattattc cactttctat cttgtctcga gcttccagca tagctgacat
     3361 aacagaccct gatgatggtc ccaccaatat cttctctttt tttgttaatt cctttaccat
     3421 tgcaaaagag tctttattag ttgcagtata ccattcatca acaacaattt cccgtctttt
     3481 gaaaagatca ggcatagaag attcttcaaa atttctcaag ccctgaagta gatgattttt
     3541 ttgtgcttga actgcaacaa ctttgatgtt aggattcttt tcttttagat agaagcctgt
     3601 acctgttata gtacccccag ttccacaacc tgttaagaaa tgtgtaagct ttccattggt
     3661 ttgttcccac agttctggac cagttccttc ataatgagct aaatagttag catcattttc
     3721 atattgattg ggcatatagt agatatctgg tctgggagtc gatattgcct tggctagtgc
     3781 aatgctctga tccgttcctg cacccactct cggacaaaga tcatcagagg tttcatgtat
     3841 tgtagctcct aaattcttca gtattgattt tgtttcagcg cttacctttt caggtataac
     3901 tatttcgact ttgtatccca tcgatgatga aatccctgtt agcgcaattc cggtatttcc
     3961 tgaagttggt tcaatgataa tactcttatc tttttttatc acacgctttc tttcagcgtc
     4021 ttttatcatc caatacgctg ctctatcttt aacagaacca aacggattat accattccaa
     4081 tttagcaaat aattttacat tctctgtcga aaatgaagat aattctatta agggagtatt
     4141 tcctattttt tctgttactt tactttcagt tattgttaaa gtcatttact ttgccttttt
     4201 tataataaat tctaaatctg cttccttttt accaattgat attaattcat gtccattcct
     4261 gtttacccac atagaaatat cttcttctga ttgggggtca tcagcaacaa cttttaattt
     4321 ttcacctatc gtcatttttt ctatttcgat tttagtccta aagacaggct caggacaata
     4381 aagccctctt acatcaatga cttttctaaa atctatatcg tcacgcatta tgaaatacaa
     4441 tataaaaact gctattgtga tataaaatgt ttatgacctt attttatagt taatcctatc
     4501 taaaaatatc tcgtgattcc tttcttacaa tttttttgat tgaactatga tgtttagaat
     4561 aaatagcatt tctaactatt gccacaggaa tcccttcact ttttcccata acaagttctg
     4621 ctgctccagc tatttcatca acaatagcta tttctgtaac cttcataatt ttaccgaaca
     4681 tatctctttt gcctatataa ttttttaaag gattaattcc tgcaatacct attgcaacat
     4741 tggtctgacc aatccgaaag gggcgaccga atgtatctga tattaaaacg gcaatatttt
     4801 ttcttgtttt ttcatatatt tcttttctta ttgaatcagc cgatttatcg gggtcctttg
     4861 gtagtagaag aactcgattt ttacttttgc ttacattact ttgatctata ccagcatttg
     4921 cacaaataaa accatgaagt gtttcggtaa ttattatatt attaaatact cttacaattt
     4981 ttcgcgattc ttgaagtatc aattcgacaa ttctggggtc cttatttatt ttcttgccta
     5041 attcaattga tctagaggaa ggaataatat tatttagata aattgaccgt ccttcatttt
     5101 ttgaaataat tttttgagct atgactatta tatctccatt tttaataatc tgattgttat
     5161 tttttatcga ttctaataga atatctaata aatttaattt cggattaata ttaatattta
     5221 tgtatattgg aattatctca attttcttta acattcgagt ttattttatt taagttagtt
     5281 tttaaaatcc agttaccaaa tcttttttct aataagcctt ttagtaatga taagttcctt
     5341 aattcaacta catcatctgc tttatttttg actaattcaa cagggcaaaa accgattgtg
     5401 taacctgcta tgtcaaatag ttttatatca ttagccccat caacaactac cacgatttct
     5461 tcttttttaa agccccattt tttaattatc ggtctaacaa caagtgattt atctgaagta
     5521 acctgtaagt caagatcatc taatattcca tttttgaaga ttaaattatt agaaaaaatt
     5581 aaatctaatc ccaattcttt ccttaatctg tctgtaataa ttgtaaaacc gcctgaaaca
     5641 gcaatcattt tccaccccgc atcttttagc gctgaacaaa gatcttttgc tcctggcatg
     5701 atttctaaat tttctcctat ttgtttcgca tcatcaaatt ttataccttt taacgccttc
     5761 actctctttc gaagcccttc ttcccaatct atgtcccctc ttattccttg ttttgtaata
     5821 tcccaaattt ccttttcttt ggaaggtcca aaaacttgag ccaaaattgg taaatattca
     5881 gcattaagta atactccttc cacgtcaaat attattaaca taaatatttg cttaagcgac
     5941 tatcttaccg tatattaagc ttgtctgatt tctaagaatt atatttaaat taccaaatca
     6001 aaatccaact ggaaactaat tataaaaagt ataggtaaaa aataaataat atggaactct
     6061 gcttttaaca atccatacaa tggaaaatgc tcaaataatt tttatttttt attccctaat
     6121 tatttttatt gtggtgggaa ttttttctac ttggatttac ctcttattct ttatgattaa
     6181 ttctcttaga aaatcaccca ggttggaaaa aattaagaat ataaacaaaa cgtttccaat
     6241 ggtgagtata atagttccgg ccagaaacga ggaaagatat attcgtaaat gcgtagatag
     6301 cttagtaaaa caagattacc ctgatttcga aataatttta gtaaatgatg aatcatcgga
     6361 taaaacttta gaaatcatga atgaatacca aaattcatac tctaccatta aagttctcaa
     6421 tgttaatcga ccagatgacg attggactgg gaaaaattgg gcttgttatc aaggttatct
     6481 aaaatcaaaa ggtgatttat tgttgtttac ggatgcagat tcttatcatt ctgaaaacac
     6541 aatgtcttta gtagtacaaa atactaaaca ttataaatta gatgcaataa cagttatgcc
     6601 taggttatta tgtaatgatt tttttaccaa agttacgctc cctctattaa ctatcttcat
     6661 gcacacccac ttttctcctt taaaggttaa tgatgcaaaa tcaaaacttg gttatttttt
     6721 tggaagttat tttttaatta agagagatac ttatgaaaaa gttggttctc atgcatcagt
     6781 taagcatgaa ataattgaag acagtgcatt aggtaaaaag gttaaagagg gcaattttaa
     6841 aatgaggatg gttaggggtg aaagctatgt ggaaacaatt tgggcacgaa atgcctctga
     6901 attatctaaa gctatagata ggctcgctat accaatgttt atggaaaaca ggttaaaatt
     6961 atctctttta agcatagtcc tgttcttttt attaatattt ccttttttga tagtttcttt
     7021 attattgact ttaagtataa ttcatccata tatactgaac tttgtgatct tactccttgc
     7081 aggtatgact ttggttattt tatttctagc aaatttaata cagatcacta tgacattatt
     7141 cttaaacaag ttttactctc tctgtttatt aattggggca acaattatcg tttccagatt
     7201 tatattcaga ttaataacat caaatagaat aagtattgta aattggagag acagaatata
     7261 taaaattaca cctaactaaa cttcccacta taatagccga cgacaaattt gaaagtgaat
     7321 attttctttc ttttttgata caaagttatt taaaaaaatt acattttcat ttttgtatgc
     7381 taaaatatat ctccaatatt catgtattga ttttttaata ccaatccttt tagttgctaa
     7441 cactaaatta ggtaaaaacc ctttttcgat atataagtcg ttatttatat ctgttaaatc
     7501 ttgatcatta tgttgtttac ctatgcaaaa tgcttgtgtg atttttcctg gtccattggt
     7561 taaaatctta tcatccacat tccgataatt tctcatgtac tgaattcctt cttttggttc
     7621 taatccacgt attaatacag cacctgcaac ttgatttatg gattttgcag tgacgttgaa
     7681 acaataatga tttccatacg taaaataaac ataggacctt cctgctggac caaacattac
     7741 catatttctt ttggttatac ctctataagc gtgacttgct tcatcacttt tgtaaccata
     7801 tgcctctgtt tctacaataa taccagagag cttaattttt ccattctgat ttcgtataat
     7861 cttaaccaat ttttttccta taagattttt agctactaaa ttagtatcca ttgaaaaaaa
     7921 attataattc agaattgatg gattcaataa attttaattt gtaaacaaaa ttattaatag
     7981 atttaggagt gagacagtca atgattgatt taaatagtct ctggtaccaa taattttgtt
     8041 tgcaaaaaaa tttagggatt aattatgaac taaaattgcc tttgatatta atacataccc
     8101 catttggact acggtttttt gacgtaatag caaattcgag tgtcggtaag atttacgcgg
     8161 cattcaatat ttatttaatg cctttgataa ctgcattagc tgttttttta attttgggtt
     8221 ctcttctagt tttattttct aatgattccg caagagaggg tattcgcgaa attggacccc
     8281 aaggcaatct gttgattcca ggattgaatc ctattctacc atggacgtac ggctggattg
     8341 gattagttat tacgataata atccatgaag caggtcatgg cattgttgcg agagtttaca
     8401 atacaaaagt tgagtcaaca ggtattgtat tgttcctagg tatacctatt ggtgcatttg
     8461 taaatataca acaagatgaa ttaaataaaa caacttttaa acaaaaaagt gccattctta
     8521 ctgcaggtcc attgaacaat atggtcattg caggtatatc ttttggatta ctttttgttt
     8581 taataacatc tcttaatcct atttctaata ttcaaggaga tgaaactggc attactgttg
     8641 tgactattgg agagaattct ttagccaaat ctattggtct ttcaaaaaat tctattatta
     8701 cttttatcaa tgctgaacct atagagaatg ttcaagaact taggaaaatc ttaagagata
     8761 atttagggac aacaatacaa atgaaatgga tatcagaaga caagaaagaa caaataaagt
     8821 cttttgaact tcctgcatct atcccagaaa ataaaggtgt cttaggggta actcttacag
     8881 atgcattttt tgatccagga ttggtattag acaattacaa aaatcttttt ttgactaatc
     8941 cattagcaat tcttatgcct cctacactgc agcaaaatct cgttccttat tctgatagta
     9001 tgtcttctta ttacacttca actatatttg gtccgacatt cccaattatt gcaaatttcc
     9061 ttttttggat ttggtttatc aattttaatg taggtatatt caatgcactt cctataacta
     9121 tgttggatgg gggtcagtgg tatggtacct tgcttgaaaa caaaattaaa actaatagca
     9181 aaataaagcc ctttacaatt ttaagtctcg tttttgtaat aattgtaata ttgtcatttg
     9241 ctctgccatg ggttttaaga tgaaagataa taactaatta tttacactta actgtaactg
     9301 aaataatatt atatgactaa aactatatta atggaaatat agataataga tatgattcaa
     9361 tttatgttta ttgagacttt attttaaaat agatatataa ttggtattca aacaaaagaa
     9421 aaaaatttga tcaatatgaa aaaaaataat ggaaacggta gtgataaaaa caattataat
     9481 aaatcattga atagcgaaac taaaagtgat tctctggata aaaattttaa gaatcaaaga
     9541 tatgttaaag atgaagaagc aataataaaa tctatttttg attccattcc aaaagagtca
     9601 caaattacta ttgctagatt tgaaggacca aatattgctc tatatactaa aaatccaagg
     9661 ttctccctta cagaattaac gtattatcta tcgtctctat caaaaacctt aaaaaaaaga
     9721 tttatagtaa gaacagatcc ctcaatcaga cttcaagaag acgaaaccag gactattgtt
     9781 tctaaaatat tacccaaaga tgtgattgtt tccgctgtat tttgtgatga tgcaacaggc
     9841 gaggtagttc tagaggttaa taaacctgaa tcagttattt ctgaaactat tataaatata
     9901 gctgaatcta caggttggat agcacattta cgacgttctc ctcatatccc atctatgagt
     9961 ataaaaaata tacacacagt tttaaaaatt tcctcaaaag aacgtagtaa ctttcttcgc
    10021 gaattaggca aaagagtttt tcgagacccg ttagtaaaaa aaggagatga aagtttagaa
    10081 agtgaaaatc cattagaaag gccatcagga aatgataaac aaagtaaaac ttggaacaac
    10141 aaggaagttt atattttctg tttaggtgga gtaaagcaag tgggaagatc ttgttttatt
    10201 gtcgtgactt cggagagtaa aattatgtta gattgtggaa ttaatccagg tgagaataat
    10261 gctattgatg cctatcctcg gatagattgg tttgatttta aattagagga tttagatgct
    10321 atagtaatta gtcatgctca tatagatcat caaggatttt tgccaacatt attcaaatat
    10381 ggctacgatg gtcccgtcta ttgtacagaa ccaacattac ctttaatgaa tttacttcaa
    10441 tcagattccg taaaaatagc acagaataat ggtgtttatt gtccatatga gattcgagat
    10501 atcaatgaaa taataaaaca ttgtatcaca ctaccttacg gaaagcctac cgatatttcg
    10561 ccagatatta caattacact taataacgct ggtcacatta tggggagcgc gacagtacat
    10621 gtgaatattt caggagccca taatattctc tattctggtg attataaata tgctaaaact
    10681 cagttattag atagtgctgt ttcaaattat cctagggtag aaactttgat tactgaaagt
    10741 acatatgggg cctcctgtga tctaatgccg gaccaaagtg tagtttataa gagtttcact
    10801 gagacgatta acaaaacatt gacaagtggt ggcaaagtcc ttattccagt gccagctgta
    10861 ggacgtgctc aagagataat gctagtttta gataaggaaa tgaaagatgg gcgattgatt
    10921 gaagcaccaa tttatatcga gggaatgatt tctgaggcaa gtgctattca tatgtcgtac
    10981 gcacattacc ttggccaaga tgtaagacga tctgtttcac aaggaattaa tccattccaa
    11041 tcagaatatt ttactgtagt taatggatgg ggaaaaagag atgaagtatt aaaggatgag
    11101 aatccagcta ttatcatggc tacatcaggt atgttagagg gcggtccttc tgttgaatat
    11161 tttaaaga
//
LOCUS       DASVEV010000281         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1511432, whole genome shotgun sequence.
ACCESSION   DASVEV010000281 DASVEV010000000
VERSION     DASVEV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511432"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF222_12350"
     CDS             <1..325
                     /locus_tag="VF222_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143257.1"
                     /translation="ILGSGNNEENMYVEPEGTIFAIVGTGGQELHGLDGQAYFTANQL
                     EDHGFLELKTTNNGKSLVGQFYSNDGGIIKDEFIIEKSSVDKTFNENTPNINPSNLNE
                     IAYKK"
     gene            complement(405..1241)
                     /locus_tag="VF222_12355"
     CDS             complement(405..1241)
                     /locus_tag="VF222_12355"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7143258.1"
                     /translation="MLTLLSFLIKLHENLRRFDSKTNSYIKNSNEKNIHDARTSIRRF
                     NAAFLVLPKRYRKGCLLSDYNQLANKFFKINSEIRDMDIIHDKLNGYPQTDQRNIVVD
                     LLKKNRQSNLEGAKTIALSLKNMNSNKILDKMDITEKELQKRYNKLLFRLISKIETNF
                     LIVITNTLKIKELHELRKDCKKLKYILELLVKDNNKNNTAIGLIKTLQNIQDILGSIR
                     DYDITIDYLKKLELSKEIQEIVNNEMEQRRLKYEEFLRFCKRRLHISRDSFFAKIKSL
                     LS"
     gene            complement(1453..2265)
                     /gene="pstB"
                     /locus_tag="VF222_12360"
     CDS             complement(1453..2265)
                     /gene="pstB"
                     /locus_tag="VF222_12360"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7143259.1"
                     /translation="MTSHAEITTNLYNETKSFDVESKLVINNLNAGFNTQVLKNIKLA
                     IEENSVTAFIGPSGCGKTTLLRCINRMHEMTPGAYAKGEIFLDEINIYDKKVNPVMIK
                     RRIGMVFQKPNPFPTMSIYDNVAAGLKLNGVKDKKIITEIVIESLEGAGLWQEVKDEL
                     NKPGMSLSGGQQQRLCIARALAMHPEIILMDEPTSALDPRASSKIEELIHELKKYLTI
                     VIVTHNMQQAARVSDYTAFMYLGELIEFGKTKQIFEKPKKEFTERYISGKFG"
     gene            2707..3114
                     /locus_tag="VF222_12365"
     CDS             2707..3114
                     /locus_tag="VF222_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143260.1"
                     /translation="MAKQKSINKKKSTGHSNNPGIYASLAENRVKYDKQIDIPNPKLQ
                     PDKTFQYTLFDTSSTGNATSPPPSAMNNVFFPELEKLSRKLNKAFEKKGAKYLLKKQK
                     ELNDNTIQISIALLNGTRYLTYTKPTVEEKEKK"
     gene            4364..>4804
                     /gene="sixA"
                     /locus_tag="VF222_12370"
     CDS             4364..>4804
                     /gene="sixA"
                     /locus_tag="VF222_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX7143261.1"
                     /translation="MRHGYAGNRLSDSMKDTKRQLTVSGKKEVVEIAKSLKKLGVKFN
                     VIFSSPLTRAFQTAKIIAEEYKLTEQIEQSEELKPNGSKNFLYNKLSKLNIDSVILIV
                     GHEPYLSSMINDIISNNDSDRNYNKNHNNNNIILKKAGLSRIKIT"
BASE COUNT         1723 a          627 c          599 g         1855 t
ORIGIN      
        1 aatactaggt agtggaaata atgaagaaaa tatgtatgtt gagcctgaag gtaccatttt
       61 tgccatagtt ggtacaggtg gacaggaatt acatggatta gatggtcaag cttatttcac
      121 tgcaaaccaa ttagaagatc atgggttttt ggaattaaaa acaacaaaca atggaaaaag
      181 tcttgttgga caattttatt ctaatgatgg tggtataatt aaagacgaat ttattatcga
      241 aaaatcatct gttgataaaa cttttaatga aaatactccc aatattaatc catctaactt
      301 gaatgaaatt gcatataaaa aataaataga aattaatcga cttttctatc acataaataa
      361 ctgataattt ttacttcaca tatatagaaa aaccatttac ttatctatga aagtaaactt
      421 ttaatctttg caaagaatga atctctggag atatgtaaac gtcttttaca aaatcttaaa
      481 aattcttcgt actttaatct tctctgttcc atttcattat taactatttc ctgaatttct
      541 ttactaagtt ctaatttttt taaataatca atagtgatat cataatcacg aatactccca
      601 agaatatctt ggatattctg aagagttttt atcaatccaa tagcagtatt atttttatta
      661 ttatctttta ccaataattc taaaatgtac ttcagtttct tacaatcttt tcttaattca
      721 tgtaattctt ttatctttaa agtatttgtg atgacaatga gaaaatttgt ctctattttt
      781 gaaattagtc tgaaaagaag tttattatat cttttttgta attctttttc tgttatgtcc
      841 attttatcaa gaattttatt tgaattcata tttttaagag ataaagcaat agtttttgca
      901 ccttctaaat tagattgtct attctttttc aataaatcaa ctacaatgtt tctttgatca
      961 gtttgtggat aaccatttaa tttgtcatga ataatatcca tatctctaat ctcgctattt
     1021 attttaaaga atttattagc tagttgatta tagtctgata ataaacatcc ttttctatat
     1081 cttttaggta atactaaaaa agcagcatta aatctcctga tcgaagttct tgcatcatga
     1141 atattttttt cattagaatt tttaatataa ctgttagttt tactatcgaa tcttcttagg
     1201 ttttcgtgta gttttattaa aaaagataat agtgttaaca agataatgtc tattaaatta
     1261 ttatgcttat aaagttaata tataaaaaat ttttaatcta cctttaatat ctacatatta
     1321 taaactaatt gttgtcttgc tttcatttta tataagtttc agaaacttat ggtttaattc
     1381 cctattttct tatctatttc tgaatttaat aaatttaata ttggacaata taattgatta
     1441 ttgtatgata ggttaaccaa atttaccaga gatatatctt tcagtaaatt cttttttcgg
     1501 tttttcaaaa atttgttttg tttttccaaa ttctatcaat tcaccaaggt acataaatgc
     1561 tgtgtagtct gaaactctag ctgcctgttg catgttatgt gttactatta ctatagtaag
     1621 atacttcttt aactcatgga ttagttcttc gattttagaa gaggcacgag gatccaaggc
     1681 cgatgtaggt tcatccataa gaataatctc aggatgcatt gcaagtgcgc gcgcaataca
     1741 aagtctttgt tgttggccac ctgagagact cattccgggt ttattcaatt cgtcttttac
     1801 ctcttgccat aagccggcac cttcaagact ttctattact atttccgtta tgattttttt
     1861 atctttaaca ccatttaatt ttaaacctgc tgcaacatta tcatagatac tcatggttgg
     1921 aaagggatta ggcttttgaa ataccatacc tatgcgtctt tttatcatta ctggattgac
     1981 ttttttatca taaatattta tttcatctaa aaaaatttct cctttagcat aggcaccagg
     2041 agtcatttca tgcattctat taatacatct taaaagagta gttttaccac atccagatgg
     2101 accgataaat gcggtcacag aattttcttc tatggctaat ttaatatttt taagaacttg
     2161 tgtattaaag ccagcattga gattatttat aactaatttt gattcaacat caaaactttt
     2221 agtttcatta taaagattag tagtaatttc agcatgggaa gtcatagttt tcttattcta
     2281 ttaaatgcta ttttaagtaa ataagagaag tccatagttt gaatataggc ctatatagaa
     2341 ctattatgct tttaataaaa aatctccaat atattctttg aaagaagcac ttaattacat
     2401 gttttctatt ttatctttta tcgtcataca ataatttttt ttaaatacta tagtgaatat
     2461 aatcatctat tgtaaaacat acataaatac taaaagacta catcttagtc ataatcacat
     2521 tcataaattt atctgtatat aacatttatt ggaaagaaat atgatactaa tggtaaagaa
     2581 gcatgtaagt taagacatat atggaaatta taggttttgg cgattattat gtataaacta
     2641 aaaatatttc accttatatt gtatcatata aaatagtttt tatattatat agatttaatc
     2701 taattattgg caaaacaaaa gagtataaac aaaaagaagt ctactggtca ttctaataat
     2761 ccgggtatat atgcttcatt agcagaaaac agagtcaaat atgacaaaca gatagatatt
     2821 ccaaatccaa aacttcaacc tgataaaaca tttcaatata cactttttga tacctcttca
     2881 actggtaacg ctacatcacc accaccttca gcaatgaaca atgtattctt tccagaatta
     2941 gaaaaattat ccagaaaatt aaataaagca tttgaaaaaa agggagcaaa atacctctta
     3001 aaaaaacaaa aggaattgaa tgataataca attcaaatct caatcgcgtt gttgaatgga
     3061 acaagatatc ttacatatac aaaacctact gtagaagaaa aagaaaaaaa ataaatcgac
     3121 gttagtagta attcaaccac ttaatggtat tttttaattt gatagttttt tattaattaa
     3181 ttaattaatt atttgtagaa gtaaataact tgtatattat catccatact tatattcatt
     3241 aattgtatca ttttatcttc tccctctttc cttttgtggc ttccattcta tgaaaaatat
     3301 tctttagcct tttgcttata tcctatggca tcatttgcta aacctagttt ataccaaata
     3361 taaggatatt tggatgtagt tccataattt tatcgtaagt ttctatttct ttctgaattt
     3421 ttaactcata tgaaatattc tcccaccatt caaaatagtt ttttatattc catcattttt
     3481 gataacaata tattcattga atcctataac cttaaaactt ggttatcatg aaacatgtat
     3541 acctatagta tttattttta gtatttagac tatatggttg ttaatgttta tggactttca
     3601 atgagtattg ttcattttta tgcaatttaa aatccatcca tcaatataag ataaggtaaa
     3661 taatgattag tattttatat attataaatt aaaatttgaa aaatttagtg tagagctaaa
     3721 attaaaacgt tagatttagt ccggagaaag taaaagaaaa gagtgggaat gacttctccg
     3781 gatgaaacaa ggtgggtttt aagtgtaagt atatagtcag ttttatttaa ttagtattcc
     3841 ctattattat tatgattgtc tatttagaaa cattatagcc tatataaaaa acattaaagg
     3901 gaaggaagga cttaaaaatt actttctatg gaaatggtct atggtatttg cttggattta
     3961 tttataacta ttaatttatt tgttttttta ataggtatta tatttaccta tattgaacta
     4021 aatttacaaa attgattgat tttaaaaaaa gaaaaaaact caatcagtta ggatactgat
     4081 tgaataactc ttttattgtt ttattaaaag ttttgttatt agttatgtaa ttaactataa
     4141 tagtagattc aggtatttat tttatttctg aatattgatt tatacaaaaa aaaactttat
     4201 catagtataa ggtaaaaatt aatttaatca ataatcaatt attttagttt ctattattct
     4261 tttttgtcct tattatcttt ctatttttgt ttcatattac taaaatggta acgactactt
     4321 tatataaaat aattaatata attttgcatt ggatttgttt attttgagac atggttatgc
     4381 tggaaatcga ttatccgact ctatgaaaga tactaaaaga cagctaactg tatctggaaa
     4441 aaaagaggtt gtagaaatag caaaatctct taaaaaatta ggagtcaaat ttaatgttat
     4501 cttttctagt ccgttgacaa gagcctttca aactgctaaa attattgctg aagaatataa
     4561 attaacagaa caaatcgaac aatctgaaga actaaaacca aatggaagta aaaattttct
     4621 atataataaa ttaagtaaat taaatattga ttctgtaatt ctcatagtag gtcatgaacc
     4681 ctaccttagt agcatgataa atgatattat atcaaataat gattctgata gaaattataa
     4741 taagaatcac aataataata atattatact caagaaagca ggtttatcac ggattaaaat
     4801 aact
//
LOCUS       DASVEV010000282         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1318761, whole genome shotgun sequence.
ACCESSION   DASVEV010000282 DASVEV010000000
VERSION     DASVEV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318761"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF222_12375"
     CDS             <1..348
                     /locus_tag="VF222_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143262.1"
                     /translation="YDSTPYASKGHIALNLPCDPKDPQDRLLDVLIGRAPDMIPMALG
                     YIPELSSPPNMCVYHGQFGFGDPVTDIVLKDVSDDPITFKGPHSVAIVTHESYIPQKT
                     SFMQQQHNMTGSQ"
     gene            complement(716..1318)
                     /locus_tag="VF222_12380"
     CDS             complement(716..1318)
                     /locus_tag="VF222_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143263.1"
                     /translation="MYYVQIPKVQYLLYFFGFIIFLYLNNYYTVYSHIPNNNIVKQEI
                     VDQYDKIKVQFAYEPEMLTINTLTDLKFSVLNSTTEEHIKNFLARIVVTEGSEVFRFD
                     NIKVNDGDFSVKHSFANYGTHQVILRVDTNSSITSASFDVIIPSSQSSSSSPFPMGSV
                     KSNLDNDKNTQNFTTFLIIGVGLAISIGMTIILLSILKKN"
     gene            complement(1628..1810)
                     /locus_tag="VF222_12385"
     CDS             complement(1628..1810)
                     /locus_tag="VF222_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143264.1"
                     /translation="MIYPGMIMQAIGIWPILLSQDIYFGILVGMSILGIGTAFVYPAL
                     LAAISDIAHPQWRATS"
     gene            complement(1996..2139)
                     /locus_tag="VF222_12390"
     CDS             complement(1996..2139)
                     /locus_tag="VF222_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143265.1"
                     /translation="MIESIDDDDEEEEEKHTIDSNSYLLNKSKNLNFKEVFIVHRRRI
                     VLF"
     gene            complement(2136..2432)
                     /locus_tag="VF222_12395"
     CDS             complement(2136..2432)
                     /locus_tag="VF222_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143266.1"
                     /translation="MLPFSIILTSDIEKPYCFAKREGLDEFAGYVAVALVGFLTGYIA
                     AVYGLKPYPFYLGIKFPIVGFLISLLLVKDTKEFTQLEIKIIKKMRKQMREIKR"
     gene            complement(2851..3267)
                     /locus_tag="VF222_12400"
     CDS             complement(2851..3267)
                     /locus_tag="VF222_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143267.1"
                     /translation="MKALAVLLFTILSFSFIVIAPLSIGSSYALKDKDGVYGIVTIDN
                     VKGKSLEVTAYIYDEDKSKQIKDSPGPFDETATQMKHTFKFDNDDLPQDVGPATQWIV
                     CIEFKNGNGEPSCWTNTFNSESQPDRITLDADYIRD"
     gene            3902..4057
                     /locus_tag="VF222_12405"
     CDS             3902..4057
                     /locus_tag="VF222_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143268.1"
                     /translation="MRSEQEIRNKVEELRKIRFQHSKNCQIPLDKCDVAKEYDLLIDV
                     LEWVLES"
     gene            complement(4210..4629)
                     /locus_tag="VF222_12410"
     CDS             complement(4210..4629)
                     /locus_tag="VF222_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143269.1"
                     /translation="MPNLDPWLNQNNNNYEDMTTKTINIFENHLLSIKTERQNMIHPN
                     SYPTYNERHQKIIEMLDWTLEKYKAAVTNTKEMNQQNEQVIINNIIEELDKKRDIAIN
                     KKKKTLLKDEVLEYGEEEDTIDYILFRVRELTGNKLY"
     gene            4762..5013
                     /locus_tag="VF222_12415"
     CDS             4762..5013
                     /locus_tag="VF222_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143270.1"
                     /translation="MKKALTFLMMTIMMVAVTTTISQTHEVYAENKAKDLCSQYDGEW
                     NNGKCKIKNDEDKTDYEDELCDDAKDTKKYPKICKDNSN"
BASE COUNT         1953 a          758 c          717 g         1869 t
ORIGIN      
        1 tatgattcaa caccttatgc aagtaaagga catatagcat taaatttacc atgtgatcct
       61 aaagatcccc aagaccgtct tttagatgtt cttataggaa gagctcctga tatgattcca
      121 atggctctgg gatatattcc agaattgtcg tctcctccca atatgtgtgt ttatcacggt
      181 caatttggat ttggagatcc tgtaactgat attgttctaa aagatgtatc agatgatcca
      241 ataacattca aaggtccaca ttccgttgca atagtcacac atgaatcgta cataccacaa
      301 aaaacatctt ttatgcaaca acaacataac atgactggtt ctcaataact attttttatt
      361 tttattgtat tgtcaacaaa tatagaaaat ataaacttgc aatctataat catatcattg
      421 agagaaactg tctatatatc cttcagagaa gaaataataa actgaaaaaa ataaatgtaa
      481 acaattgtaa tgtatatata gtaatttttt caaaaacaga taaattttta aaacaaaaaa
      541 attaaataaa tccacctgta tttcatagaa gattctccat taactataat aatagattta
      601 atagaagaaa catgttttaa gataatatgt tctattatta gcagaaatgt aacataaaag
      661 atataagaaa tgaatatctt agacaagaaa gaatattcga tataaagtgc aacacttaat
      721 ttttctttaa aattgataat aatattattg tcattccaat acttatcgca agtcctactc
      781 cgataattaa aaaagttgta aaattttgtg tatttttatc attatctaag tttgatttaa
      841 ctgaacccat aggaaatgga gatgatgatg atgattgaga agaaggtata attacgtcaa
      901 atgaagctga agtaatagaa gaatttgtat ctacccttaa aataacttga tgagttccat
      961 aattagcaaa agaatgtttt acagaaaaat caccatcatt cacttttata ttatcaaatc
     1021 tgaatacttc tgacccttct gtaacaacta ttcttgctaa gaaattttta atgtgttctt
     1081 ctgttgttga atttaaaacg ctgaatttaa gatctgttaa tgtatttata gttagcattt
     1141 ctggctcata cgcaaattga actttaattt tgtcatattg gtctactatt tcttgcttga
     1201 cgatattatt gttgggaatg tgagaatata ctgtgtagta attatttaaa tataaaaata
     1261 ttataaagcc aaagaaatat aacaaatact ggactttagg aatctgtaca taatacaata
     1321 tactatcaga gatgtatcaa ttttaattac tttaatttat ttgtaatata tatggctctt
     1381 gttgatgaga taatttatta tctacaaagt aaaaaagaat aatataaatc gtgaatgata
     1441 attttattct gatttgtata ttggtctttt agtttcatgc ataactataa aaacaaaaaa
     1501 ttccagaacc gaaggctatc catccagcta tttgaattgc tatatttata ccaaagaaat
     1561 ctgcgataga acctatacct aatgcaccaa ttacaaatcc taaatctctc cagaatctat
     1621 aaacacctca tgatgttgct ctccattgtg gatgagcaat atcactaata gcagcgagta
     1681 aagcaggata cacaaatgca gttcctatac ctaaaataga cattccaact agtataccaa
     1741 aataaatatc ttgagataat agtattggcc aaattcctat tgcttgcata atcattcctg
     1801 gataaatcat ttttttgtcc tactaaatca ctaaatggac ctgtagcaag ttgcaaaaac
     1861 ccccaaactc caggatgcag tgcttttaaa tatgcagtat catttacact tattccaaaa
     1921 gatacaaaat aagggtaaac aatccccaag atacaccaaa tactaaatta ttaacaagcc
     1981 atgcttggct tacagctaaa agagaacgat tcttcgacga tgtacaatga atacttcctt
     2041 aaagttaaga tttttacttt tgtttagcaa ataggaattg ctatctattg tgtgtttttc
     2101 ttcttcttct tcatcatcat catctattga ttctatcatc tttttatttc cctcatctgt
     2161 tttctcatct ttttgataat ttttatttct aattgggtaa attcttttgt gtctttaact
     2221 aataataaag aaattaaaaa tccaactatt ggaaatttaa ttccaaggta aaatggataa
     2281 ggctttaatc cataaacggc ggcaatgtaa ccagttaaaa aacctactaa agcaactgct
     2341 acatatccag caaactcatc aagtccttct ctttttgcaa aacagtatgg tttttcaata
     2401 tctgatgtga gtataatcga aaatggtaac aaatgttttg taaatttttt cctgctagta
     2461 agtaatatta caataaatat ttttatataa ttaaaataaa aataaatttt tttctttgta
     2521 acttagaaaa atagaaaaat agtaagcgga taaaagattc tttacattta gacaaatttt
     2581 attatgttaa atttaagaat gtctcttgta aaaaagcaat aatacaatta aatgatacaa
     2641 atttaaatta ataataataa aactataaaa gaaatactat attctatttt gtaatattga
     2701 ttgaaagaat caagttaagc aaaataagca agcaaataaa acgaaaaata tcgactaaat
     2761 tgaaaaacat aatataataa aaaagtatgg tcgattaaaa attatacctt ttgattgatt
     2821 taattggttg taaaaaaatt aaaaaagaga ttagtcgcgt atataatctg catccagtgt
     2881 tatcctatct ggttgtgatt cagagttgaa ggtatttgtc caacacgatg gttcaccgtt
     2941 tccatttttg aattctatac aaacaatcca ttgtgttgct ggtcctacat cttgaggcaa
     3001 atcgtcatta tcgaatttaa atgtgtgttt catttgtgta gcagtttcat cgaaaggtcc
     3061 aggactgtct ttgatttgtt tactcttatc ttcatcatat atgtatgctg taacttctag
     3121 acttttccct tttacattat ctattgttac tatgccatat acgccgtctt tatcctttag
     3181 tgcatatgag cttccaattg aaagtggagc tataactata aagctaaagc tcaatatagt
     3241 aaatagtaat actgctaatg ctttcatact atgttataat aaatatatgt ctatatatat
     3301 actacgttaa atatttcaga atattatagg gaaataattg taagagattt aggacaatca
     3361 tgcatatttc cactatgcag aaattgcata taacttgtaa aattagtttt tttaaatctc
     3421 taatagtatg ttatgtatgt acttcatatt ttagtatttc tttgcatctt tacaatcaat
     3481 tatatccttt atcttgacat ataatatatt actaagtttt tgcagctcct ccctttcaga
     3541 aatagattac aagcaactca caacaaacct tttgatccaa aagaaagagt tagtagttat
     3601 cttaaaagac ttccaaaagt cctaccaact ctttagaaat taaaaatatc gatataaggt
     3661 atttgcaata attactaaac aagaattaag gaatagaaaa gtaagattga ggctgaaaat
     3721 actatcaaaa tagatgaatt gtttgagaaa gctgatattt tatcaaatca aaaaaattat
     3781 gatgagtaaa tcaaatatat caaccagaat caactgtaaa aaacatggaa ataaatgaat
     3841 gtgtttttgg aatggctctt gcaaacaatg gtttagatac ttatggatta gatgatgagt
     3901 gttgagaagc gaacaagaaa ttcgaaataa agttgaagaa ctacgaaaaa tcaggttcca
     3961 gcattccaag aactgtcaaa tacctttgga taaatgtgat gtggcaaaag agtatgacct
     4021 acttatagat gtactggaat gggtcttgga aagttaaaat agttttaaaa gtatatcttt
     4081 agtttatgta ctttattacc ataacttagg ctcttatttt tgcaccgcta tcggtattac
     4141 tcctcccgac gggcccgtag ttttctcggg ttcacctatt aatcgagaga gtaaatcctt
     4201 ttattttttt taatacaatt tattacctgt caattcacga actctaaaaa gaatgtaatc
     4261 aatagtatct tcttcttctc cgtactccaa aacttcatct tttagtagag tttttttctt
     4321 tttatttata gctatatctc tttttttatc aagttcttca ataatattat ttattattac
     4381 ttgttcattc tgctggttca tctcttttgt attggtgact gctgctttat atttctccaa
     4441 agtccaatct aacatttcta taattttttg atgacgttca ttataggtag gataagagtt
     4501 aggatgaatc atattttggc gttcagtttt aatagatagc aaatgatttt caaatatgtt
     4561 tattgtcttt gtagtcatat cttcataatt attattattt tgatttaacc aggggtcaag
     4621 atttggcata gtgcatatac aaaaaaggta tttataaatt tggtagttac ctcgagagag
     4681 gagagatgaa aattcaaaac atttaaattt ttcataattg ttctcgcaaa cttctttata
     4741 gtattaatta ttgataataa catgaaaaaa gcattaacat tcttaatgat gacaattatg
     4801 atggttgcag taactactac tatctcccaa acacatgagg tatatgcaga aaataaggct
     4861 aaagacttgt gctcacagta tgatggcgaa tggaacaatg gtaaatgtaa aataaaaaat
     4921 gatgaagata aaacagatta tgaagatgag ttgtgtgatg acgctaaaga tacaaagaaa
     4981 tatccaaaaa tatgtaaaga caattctaac tagctaaaca attaatctaa tatttttaaa
     5041 tcctattttt attaaataat tcgatattcc atttcaaaaa taaattaaaa ataagtttct
     5101 aataataaaa gctatataca aggtaatagt atctctacat tggactcctc ttttagaacc
     5161 gctatcggta ttaattcttt aacagattaa ataaaataga atattttcta tttaaacttt
     5221 aattataaaa tggtctatga ttatatacaa tccaagtcca attaaaacaa atggtaaaat
     5281 tatatgacca atgcgtt
//
LOCUS       DASVEV010000283         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1124234, whole genome shotgun sequence.
ACCESSION   DASVEV010000283 DASVEV010000000
VERSION     DASVEV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124234"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF222_12420"
     CDS             <1..219
                     /locus_tag="VF222_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143271.1"
                     /translation="ESAKNIYIGGNKDKPIYYDEDNPNDPTIKSFNLKKMKIEESVTE
                     DNIMRLTLLIDEEQENLKKKDKNDSYWK"
     gene            216..488
                     /locus_tag="VF222_12425"
     CDS             216..488
                     /locus_tag="VF222_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143272.1"
                     /translation="MTSNQTNNIYPKSCVYNCGIHIYWITATSEYWEVFIKKKHICPN
                     RTNKPATNTTTISTGESSIPTYYNKKPWNSQPKPKISNSLELLTVQ"
     gene            complement(949..1236)
                     /locus_tag="VF222_12430"
     CDS             complement(949..1236)
                     /locus_tag="VF222_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143273.1"
                     /translation="MIDQVNLLTSTGSFAVKFIELNQNSTFAGLPAVKIVNDQNFMLG
                     NNPTLATLMRVVALSEDRSKTYGVTYKIDIPNFEEYLPTVEKFIDSIQIIN"
     gene            complement(1538..1972)
                     /locus_tag="VF222_12435"
     CDS             complement(1538..1972)
                     /locus_tag="VF222_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143274.1"
                     /translation="MKLQLLSKICYLLVIGIFSASQNCVYGQEYTTAGVEDKTVELLS
                     PKVKSGEYSDKLIEQVHNNIDKNVEYVQIIATFYDQNEGMFGSKSTYAQPSDLKQNMK
                     APLEMFLDDDVANELSAYDVTLTWRHPRETEEYSNIYELSQQ"
     gene            2065..2364
                     /locus_tag="VF222_12440"
     CDS             2065..2364
                     /locus_tag="VF222_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143275.1"
                     /translation="MEKLDPDHMDGIIINERDREGLKPFLPIEENHDFAINTINRHKM
                     RLKFEPKMGELTYTFRRYTRMCRCLIPINENYYLILTIDFDQYDFDKIIMEKIIH"
BASE COUNT         1238 a          501 c          486 g         1258 t
ORIGIN      
        1 gaatctgcta aaaatattta cattggaggt aacaaggata aaccaattta ttatgatgaa
       61 gataatccga atgaccctac gatcaaatct tttaatctta aaaaaatgaa aattgaagaa
      121 tcagtaacgg aagacaatat aatgcgcctt actctgctaa tagacgagga gcaggaaaat
      181 ctaaagaaaa aagataagaa tgatagttat tggaaatgac ttctaatcaa actaataata
      241 tttatccaaa atcctgtgtt tataactgtg gtattcatat ctattggatt actgcaacaa
      301 gtgaatattg ggaagttttt atcaagaaga aacatatttg tcctaatagg accaacaaac
      361 cagcaactaa tactacaact atttcaacag gagaatcaag tatacctact tattataata
      421 aaaaaccctg gaattcacaa ccaaaaccaa agatatccaa ctcattggaa ttactgacgg
      481 ttcaataaca gaaatttaaa agaaatatga aattttatca gatattatta ctgagtatgg
      541 tggtaagact catggttgtc aaagagatag agatccaaag actggtagta tcgacttgct
      601 agtctattac gaagttccag aaggaaagag agatgaagta aaacaaaagt ttgataatac
      661 tggaaaccaa actgttaact tgataaagaa atgaatttct ataatgatga cagcagaaaa
      721 atatcaaaac tacaaaaaat tttgattatt attagtttta ttatcattat agtttggggt
      781 atttattttg tcatccatat aattaataca atagaaaata agccagtatc taacagtttg
      841 atttattcat tatcttcatc tctaatctgg aaatgtatga caaatgtgtt cgtatgacca
      901 aaccgcaata taactgctag aaaccagatt gatttacaaa agagattatt aatttattat
      961 ttgaattgaa tctatgaatt tttctacagt tggcaaatac tcttcaaaat taggtatatc
     1021 tattttataa gttacaccgt atgttttact tctatcctcc gataatgcta caactcgcat
     1081 aagtgtagca agagtgggat tatttccaag cataaaattt tgatcattta caattttaac
     1141 tgctggtaaa cctgcgaagg tagaattttg attcaattca ataaatttaa ctgcaaatga
     1201 accggtagaa gttaatagat ttacttgatc tatcataagt ttattcagat tattaacatc
     1261 ttgttttgta ttatggacgt atattgataa ggcaccatct ccaactaaat caaaagaaat
     1321 tggacgatgg tcgtcatcag aagttagatt tctcttcttt cattttttct tcccaatcat
     1381 caggatactg taatttaatt tttaataatt cactctcata ttcatttaat tcttgactat
     1441 aaacagaagt aattaaaata gaagcaaaaa gagagattaa aattataata tattttaaca
     1501 gtacctattc ctctacttgt aattctactg gctgttgtca ttgttgactt agttcataga
     1561 tgttagaata ttcttcggtt tctctaggat gtcgccatgt tagtgttaca tcataagcac
     1621 ttaattcatt agcaacatca tcatctagaa acatttccaa tggtgctttc atattttgtt
     1681 ttagatcact tggttgagca taagttgatt tacttccaaa cattccttca ttttggtcgt
     1741 agaaagtagc aataatttgt acatattcta cgtttttgtc aatattatta tgaacttgtt
     1801 ctatgagttt atcggaatat tctccacttt tcactttggg actgagtagt tcaactgtct
     1861 tatcttccac tcctgctgtt gtatattctt gtccataaac acaattttgc gatgccgaaa
     1921 aaattcctat tactaacaaa taacaaattt ttgatagtaa ttgtagtttc atatacagaa
     1981 tttttaacga attaattaaa gttttttata gattgtatta tctaaaatac tgaaatttgt
     2041 gaaaattttg aagatgcgaa ttcaatggaa aaactcgatc ctgatcatat ggatggtatt
     2101 ataataaatg aacgggatag agaaggacta aaaccctttc ttcctataga agaaaatcat
     2161 gattttgcaa ttaatacaat aaatagacat aaaatgagat taaaatttga accaaaaatg
     2221 ggtgaattaa cttatacctt tagaaggtat acaagaatgt gtaggtgttt gattccaatc
     2281 aatgaaaatt attatttgat tttaactata gattttgatc aatatgattt tgataaaata
     2341 ataatggaaa aaattattca ttaattaaac aagaaaaaga gaattctaat cagaagaatt
     2401 aattttattc cacgaactaa tcgatagatg tttattgtga tttatagttg ctaatttgta
     2461 ttagttaaac caattctgaa agtgagttgt ataattcttt gttactaact ctagtaacag
     2521 ttttacttta aaaacttatt tgtgtattat ctcaactact tactctcgcg atagtaataa
     2581 attaatgaat taaataaaat atgtacatca aaaaccatag tctgtatatt tgtattatgc
     2641 cctagcgtta ggcataaaat acagactatg gcttccttga caggaaatat gcctatggtg
     2701 ataggtatta tccttctgtt gtacaagata tttaaaaatc agtaaacaag gttaacgatc
     2761 aatcatataa ttcacttgtc atagccgaac taatagtgaa atccgggttg ggatcattgt
     2821 tccgactatg acaaatcaag tgattggttg agatatgata tatttggtag tattaacata
     2881 gtgattcttt tatttatgat tagttgtaaa tctagtttga gaatttaatc aataatcaat
     2941 aatgaatcat aaatcaatta atatcatcat tcttattatt tgataaattt gtaacttgta
     3001 ataattgatc tgaaaatttc ttatcattta acaactttac cttaaaacag acatcagata
     3061 tccagacccg ttgatgatga ttaggacata tctatgctgt tgttgtatta ttgccagttt
     3121 agtggctcta ttccaacacc tactatgaaa tttatcgtcg acatagtttt cacatctcat
     3181 tttttttaac ctaacttcct tcttcttatt actactccta ttcctcttct ttttgttagt
     3241 cttataactt gtttatcata tttcttttat tattttgcac aatctcaata acctggattt
     3301 tatctatctt tgcaatctat tattctcaca ttcaaactca tagtaaaatt atggttattt
     3361 actaggattc aacttgttac ttgttccact ttcttttttt tgataaaact tacttggcca
     3421 ttaatttcta aatatgacaa tttaatatcg gaaatgtcat cagttccact tagtctcaaa
     3481 tag
//
LOCUS       DASVEV010000284         6654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1319070, whole genome shotgun sequence.
ACCESSION   DASVEV010000284 DASVEV010000000
VERSION     DASVEV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319070"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..660
                     /locus_tag="VF222_12445"
     CDS             481..660
                     /locus_tag="VF222_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143276.1"
                     /translation="MSDSEENNNQNSSEELESAQENPKEIGSAESDPSTTEPAGDLRE
                     KAAENTGEDDSKAPA"
     gene            830..955
                     /locus_tag="VF222_12450"
     CDS             830..955
                     /locus_tag="VF222_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143277.1"
                     /translation="MVEKELIEQKIGEAIGLEKAAQKAVENLKSKGLIKAEHEKN"
     gene            961..1314
                     /locus_tag="VF222_12455"
     CDS             961..1314
                     /locus_tag="VF222_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143278.1"
                     /translation="MKEEANKQQREMEELIEEIAENEGLDVENINSVSEETAQKASKI
                     METYLGEDPDTQEPLEFLCLAEGAEVTHYEVLTSVAKEVKSRKFGTQVRNILKEEHNH
                     LEVCTKLAKQNVSKE"
     gene            complement(2800..3009)
                     /locus_tag="VF222_12460"
     CDS             complement(2800..3009)
                     /locus_tag="VF222_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143279.1"
                     /translation="MNQPYFIITLIAALTIGVLSINAGITFAQEKNETSEIDDSPLIT
                     IPQIDESMETNNTSPQDEDKIPLIK"
     gene            3277..3441
                     /locus_tag="VF222_12465"
     CDS             3277..3441
                     /locus_tag="VF222_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143280.1"
                     /translation="MDNDKDIEIKKEEDLGLDEKIKAGAKAVGRKIGDPESDIGSEYD
                     TEKTIERVED"
     gene            3789..3965
                     /locus_tag="VF222_12470"
     CDS             3789..3965
                     /locus_tag="VF222_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143281.1"
                     /translation="MHKSAITNSLFLMMGALIILVPFTSITFPNVKAQEYGNYNDDDG
                     YSTYPSDDKKLNVK"
     gene            4030..4221
                     /locus_tag="VF222_12475"
     CDS             4030..4221
                     /locus_tag="VF222_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143282.1"
                     /translation="MALSILSKKPSIKHVLKWIFSGLSVSFIAMLVIMLAHDSVTGTS
                     SQTAEYCAKYGILASPSCW"
     gene            complement(4447..5436)
                     /locus_tag="VF222_12480"
     CDS             complement(4447..5436)
                     /locus_tag="VF222_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143283.1"
                     /translation="MQKLLLIVFFLTVVLITLITFNMNFYSKAIAQENGYDDYSYYNY
                     ENNGYVEGSNNFFYNNNYQNDGYFEEGFNDINKLIVKVKIYLQNLERTGFLRISSLLN
                     GEEIVKDIVLSEIDKSQQTITVDLKVNKKNDIVEASSNDEYHVCAYHVKDLIFEYDSF
                     TNFDCNEGDVLSVDMPNYISLFKPTSQVYLKSVNYHYYALNNIGEFDNNNYYSYDRYS
                     MKYYDDEKVIIKINSPLIDRIDTKKLKVMAMIKGQIQSEVIDDVQAELDKSKDNTISR
                     TFIFDRNTDIGLIHIGDKFLACVASDDLRPPEGQECEKRILKKFGEPNVLYAR"
     gene            complement(5832..5957)
                     /locus_tag="VF222_12485"
     CDS             complement(5832..5957)
                     /locus_tag="VF222_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143284.1"
                     /translation="MKQKYFLITLIAALTLGVLFINAGITFPQEKNETPEIDDSP"
     gene            complement(6343..>6654)
                     /locus_tag="VF222_12490"
     CDS             complement(6343..>6654)
                     /locus_tag="VF222_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143285.1"
                     /translation="YGSPTNTDDKVNPDNEVGQPLGQQSEQEDEDSVQVDQQSGQQVY
                     QQTIGTNQSEQQTIGTNQSEQQTIGTNQSEQQTIGNEVNQTEEQQNTGEQVDQRSSQQ
                     P"
BASE COUNT         2473 a          923 c          927 g         2331 t
ORIGIN      
        1 gcgcaaatac aacaaaggat gattcataat caccatcatt gtttctaatc atggtatatg
       61 taactataga taatgatagc acaagaaata ttgtcattat aactttagtt atattattat
      121 tgaatgatga tgatgatgat ggcattgctc ttgttgtaaa cattatcatc gttattctta
      181 attctattat aaatatttaa aataactagt tatttatatg ctataagaga ttgtattgga
      241 gtttagaaat tgatgtatta taaatagctt tcaatttgaa taacataaat agaatattcc
      301 tagctatagt ttttactgct ctccttaaag agccgaattt aatatcaaat attatcaaat
      361 cacaatcaga ttttgagtat gaacataatg tattgaagat actaatatag tgtatattct
      421 atataatatt aaaaatagta atacaacaac atctttaaac tttgttgtcc atagataatc
      481 atgagtgatt cagaggaaaa taataatcaa aatagtagcg aggaattaga atcagcacaa
      541 gaaaatccta aagagatagg ctccgcagaa tcagaccctt caactacaga gccagcagga
      601 gatttacgag agaaagctgc agaaaacaca ggtgaagatg attctaaagc accagcataa
      661 acatttttac atttattttt tgttatcaaa attatatttt agtaggagtt ttagaggata
      721 attctcctat taaaattcca acactatgaa ataagttctc taatacttta ttatatcatt
      781 tattaatatc gttataaact ttattgcaat attaacaatg aaaatataga tggtagaaaa
      841 agaattaatc gaacaaaaaa ttggggaagc aattggttta gaaaaagcag cacaaaaagc
      901 tgtagagaac ctaaaatcaa aaggattgat aaaagcagaa catgaaaaaa actagtacct
      961 atgaaagagg aagcaaacaa acaacaaaga gaaatggaag aactaattga agaaattgca
     1021 gaaaatgaag gacttgacgt tgagaatatt aattctgttt ctgaagaaac tgcacaaaaa
     1081 gcatcaaaaa ttatggaaac ctatctagga gaagaccctg atacacaaga gcctttagaa
     1141 tttttatgtt tagctgaagg tgctgaagtt actcattatg aagttcttac ttctgttgca
     1201 aaagaagtaa aaagtagaaa attcggaacc caagtaagaa acatattgaa agaagaacat
     1261 aatcatttag aagtctgtac aaaattagca aaacaaaatg ttagtaaaga atagtaaggt
     1321 acgatagtgc cttgggattg ataatgatga tggaaagata aaatgagcga taataaaata
     1381 atcaaatgtc ttaattgtgg ttcatcttta aacaataatt attatagtag taatttttgt
     1441 tctggacatt gcaaagatgc ttacataaga catataacct gataaaataa ctaaaaaaag
     1501 gatttaatat ttccattttt ctacactagc aagaaaacaa gacaggtaaa ttataaatgc
     1561 ccagtatcta tacaattttt taactcttta agagtagact tttgccattc aggccatctt
     1621 atgtaattga tatttttatt ctcaatgtgt taattttaga aatttattaa aggtaaaata
     1681 aaattttcat caattcatag ctaggagttg attgcaatta ttaatcttat aaaggttatg
     1741 attttagtac tctataaatt agaattgtat tgaaatttct atatcattcc accgaaccaa
     1801 ttagtttatt attctacagt tatatatttt catattaaat aagttctaat aataaaagct
     1861 aaatcctgtg tagtagaacc cactacattg aaggctttac tccagaccag cttccttaat
     1921 atgctgaaga attttttggc gccgaaaaat tagtaaatta aagctaaaag ctcatttact
     1981 aatgaaatcc ttaaatttga taaaaaatcc ttaaatttga taaaaatcct taaatttgat
     2041 aaaaatcctt aaatagactt tagtctatat aacagttgag gaatagattt ggaacctctc
     2101 cggtgattaa atcccaccga gttattacaa ttgagtaacc gccgttgaac aacaactaat
     2161 tacacgaaaa tgcaattact cctgctactt gtactagtag taggagtatt cctaccaccg
     2221 ttgaacaaca actaattaca cgaaaatgtg caattttttt actagcagga ataattacaa
     2281 tttactttaa atactatatc tatataaaca tatccttata aaaccttaaa caacaactaa
     2341 ttacacgcaa atattgcact ctttttagca attgtactat tcgcaggaat aattgctcta
     2401 tcttcatcat ttatggtcgg cagtacacaa gccgaattag ataatatgtt ggacacgacg
     2461 acaagaaatc atattgaaat gatacttacg agttaaaaga catatcaaat ggtaaagaca
     2521 accctctgaa ctcaataagc aaatgaaatt ttttatttat ttcaagaaca ttttaataag
     2581 tttctaataa taaaagctaa atacaagatt gtaatagaac cctagtacat ggacgatttc
     2641 actcccgacc agcttgctta aaatgaagta gaacccccct ctttttaaaa tatatgacca
     2701 aattatagta ttatgttcag cgtattatat taaataattt gattatcaat atggaaagat
     2761 aaaatttaaa aaatactttg aaatttatta tggaattatc tatttaatta aaggtatctt
     2821 atcttcatct tgaggtgaag tattgtttgt ttccattgat tcatctattt gtggtattgt
     2881 aattaaagga gaatcatcaa tttctgacgt ttcatttttt tcttgtgcaa aagtgattcc
     2941 tgcatttata gatagtacgc ctattgtaag agctgcaatt aatgttatga taaaataggg
     3001 ttgattcata agattctact aataatcatc ttttttaaat attgtctttt gaacataata
     3061 aaagctaaat acgatgtaat agaaccccac tacatggagg gcactactcc cgacgggccc
     3121 gtagttttct cgggttcact atatttgaac tgttttagct taaatctatc aaaattttag
     3181 ttgaataaca atcaacaaat gaaaaaaaat cattagatta tatttcctct atatatccta
     3241 aattattatt aattataaag tataacaaca cttaccatgg ataacgataa agacatagaa
     3301 attaaaaaag aggaagattt aggattggat gagaaaataa aagcaggagc aaaagcagta
     3361 ggtagaaaaa taggagaccc tgaaagcgat ataggctctg aatatgatac tgaaaaaact
     3421 atagaaagag tagaagattg agaaaaaata aagatgaatt gatagatgaa tatttatcaa
     3481 tttaatagat acataaagaa agaagaaggg agagatagta aatctggaaa gttgctgctg
     3541 cttatttcat aactatatca accaaccaac tgaataaaaa gacataagat tgtagaatga
     3601 gacatatatt agactttgta acaaaaaatt ccacaaatca ttttcacaat agatttaact
     3661 aataaaaatt agtaactgaa aattacaata aacattcact tagcaacttc actaatctaa
     3721 tttatttata ctatattagc aattgtttct aaatacttca attatatttt atcttattta
     3781 aaactacaat gcacaaatca gcaataacta atagtttgtt cttaatgatg ggagcattga
     3841 taatattagt tcctttcact agcataactt ttccaaatgt aaaagcacaa gaatatggta
     3901 attataatga tgatgatggt tatagcacat acccatcgga cgacaaaaaa ttgaatgtaa
     3961 aataatttag gaaatatgtt aaaaaaaatg aattagataa attaaataga attatttttc
     4021 atactgatta tggcattatc gattttatct aaaaaacctt ctattaaaca tgtattaaaa
     4081 tggatattta gcggattgag tgtatctttt atagcaatgc tagttatcat gttggctcat
     4141 gattctgtta caggtacatc ttcacaaaca gcagaatatt gtgctaaata tggcatactt
     4201 gcttcaccgt cttgttggta atctagagtg ttctcttgaa ttaacatcca tcaatttttt
     4261 tctaattcat ctggttttat ttaatatata tcttgacaat attattttta gaatatttat
     4321 tataatttta aaataagttt ctaaataata aaagctaaat acaatgtagt agaaccctac
     4381 tatttgaagc tgaacccgat taatgaaatt aaaatttgta tttataatga aatgatatag
     4441 taagaattat ctagcatata acacattggg ttctccaaat ttctttaata ttcttttctc
     4501 acattcttga ccttctggtg gtctcaaatc atcactagca acacatgcaa ggaatttatc
     4561 tccaatatga attaggccta tgtcagtatt cctatcaaaa ataaatgttc tacttattgt
     4621 attatcctta ctcttgtcaa gttctgcttg aacatcatct ataacttctg attggatctg
     4681 gcctttaatc atagccatta cctttaattt tttagtatct attctatcta tcagaggtga
     4741 atttattttt attataactt tttcgtcatc ataatatttc atactatatc tatcgtaact
     4801 ataataatta ttattatcaa attcaccaat attatttaat gcataataat gataatttac
     4861 actttttaaa taaacttgag atgtaggttt aaagagactt atgtaattgg gcatatctac
     4921 tgacagcaca tcaccttcat tacaatcaaa attagtaaaa gagtcatatt caaatatcaa
     4981 atctttaaca tgataggcgc atacatgata ctcatcgtta gaacttgctt ctacaatatc
     5041 gtttttctta ttcactttga gatcaactgt tatagtctgt tgagatttat ctatttcaga
     5101 taatactata tcctttacaa tctcttcgcc atttaaaaga gaactaattc ttaaaaaacc
     5161 agtcctttca agattttgta aataaatttt aactttaact attagtttgt ttatgtcatt
     5221 aaatccttct tcaaaatagc catcattctg atagttattg ttgtagaaga aattattact
     5281 tccttccaca taaccattat tctcatagtt gtagtaggaa taatcatcat atccattttc
     5341 ttgagcaatc gcctttgaat aaaagttcat attaaaagtt attaaagtaa tcaacacaac
     5401 cgtcaaaaaa aatactataa gtagtaactt ttgcatatgt attctctgat tctataatat
     5461 atttaaatta ggtaattaca tggaggaaca tattatatat taacagatta gagaacgaac
     5521 tatattagat tccatgttat ataattgtat aagaaaacta ttacataaaa gagtattata
     5581 aagtagatta tcctttttga aagaagtatt taacctgatt ttgcagcaaa taagcctaac
     5641 ctcaaataag atcttaatta ttaaaatttt taatagatgt tatttttagg tatacaatat
     5701 gaaattgttt taatctagtc atatcacaaa caatataatg caaatagatt ttatctattc
     5761 aattatagat atattgttag ccatttgaga cgtattattt tctaaacttt catctatttc
     5821 tgtagttgta attaaggaga atcatcaatt tctggtgttt catttttttc ttggggaaaa
     5881 gtgattcctg catttataaa tagtacgcct aatgtaagag ctgcaattaa tgttataaga
     5941 aaatattttt gtttcataag aatcttttaa taataatcta ttttaaatat tgtctttgaa
     6001 aataataaaa gctaaatctc attatagaac cgtactacgc gcaggactct actccagaca
     6061 atcttactgt tgttgtaagt gactcaaagt gtcaaagaaa ataaatcatt taacataatt
     6121 gtagccttta ttgatatcaa attataaaag ttaataggtt ttattgttga taaatcaatt
     6181 gatttcaaaa agaatattta tattgcaaaa ttgaattgca atttgtttaa ttacttcttg
     6241 aacatccgaa gactcttcac tttctgaggc atcagacatt tcggcaatca gagttacggc
     6301 ttgaactatt ttttgttcaa catctattcc aaatgcttgt tgttatggtt gttgagatga
     6361 ccgttggtct acttgttcac ctgtgttttg ttgttcttca gtttggttta cttcattacc
     6421 aatagtttgt tgttcagatt ggttagtacc aatagtttgt tgttcagatt ggttagtacc
     6481 aatagtttgt tgttcagatt ggttagtacc aatagtttgt tggtatactt gttgacctga
     6541 ctgttggtct acttggactg agtcttcatc ttcttgttcg gattgttgac ctaacggctg
     6601 gcctacttca ttatctgggt tcactttatc atctgtgttt gttggtgagc cata
//
LOCUS       DASVEV010000285         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1709308, whole genome shotgun sequence.
ACCESSION   DASVEV010000285 DASVEV010000000
VERSION     DASVEV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709308"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VF222_12495"
     CDS             complement(<1..1128)
                     /locus_tag="VF222_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143286.1"
                     /translation="MSTITISFVIILISITFSKSYSQDLDCIYNETDWDGDGIPNDWE
                     TRGIDINNDSIIDYGLSSYNVSPLRKDLFLEIDYMKHHDIFEGVRERIIQAFKDSGVC
                     NPGEEPDGINLHIQYDQEIPHQPVIPTYSFVGSDYVRTWKGFDDLKNKYFGTKDEKDD
                     NPNVNNTLLAKSKIFHYVIFAHAVDFVGNAMSGLSHGIPGMDFIVSLGNWKSGGANVI
                     GHYNGNANHQAGTLMHEFGHNLGLGHGGGDGINCKPNYLSIMNYLRQMPVILPPAFYK
                     LDYSRIELNPLNESSLDETKGITTKIPLEIPDVLIYGIYGNQFNFVKRAIDWNYNNNF
                     ESNIPQDINLFRGIDDCAKNTPGQFLKGYNDWDNLIFITNQS"
     gene            complement(1408..3060)
                     /locus_tag="VF222_12500"
     CDS             complement(1408..3060)
                     /locus_tag="VF222_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143287.1"
                     /translation="MAIRKQVKYLKMVPLVIFAISFSLFAFPNIQYNAFAQNNDTSSE
                     MLTAEINGTGTVQPSEEKTKYEGFSEPQAPSLEQLKAFEEEITEEEREYPQQFQRESE
                     GAQGLAESGLDESTATDVLVSPPALNSNITEDISNSNASSNTITKNATLATTLTLYKS
                     TLVSPTQYRSTVTEPSVANNGPNVFYTGNWFAARSADYGSTWNYVNPLQGMSDFCCDQ
                     DVIYDKNYKIFIWFRQGIKDVNGENRFILSISKDAQTWTSYSIHPTVVNQNWKNSWWD
                     YPHLALSNNKLYITSNMFDKNDNFVRSVITQWDLKQLSSGSTVNFQYYYETNQFNYTP
                     VQGATDTMYWAVHNTNAQMKLYKWAESTNTINIAYRNTPAFQWGAMSCPGPDGLNWCA
                     RSDSRILTGWINNGEIGFAWNAKQGGAFTYPYVDVVIFQEAGLTYKSNPVLWSSNIAW
                     MYPFFSPDSNGNIGFVSYYGGGSYYPSIAAGIINSASGTPPPYPAYNIVSGKNGADRW
                     GDYDRTRPLSGVGPLFEGSASTLQGCTTGSCLEPRYFIFGQS"
     gene            3432..3818
                     /locus_tag="VF222_12505"
     CDS             3432..3818
                     /locus_tag="VF222_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143288.1"
                     /translation="MSNGNIYEKQNDHKNYSDSGIQGTINFVGSDCPPFSKKQTPPCS
                     GPYPDLPISIYTDDNEKKRIATTHTDSNGNYHIFLKPGKYIIYSSSESLLPVTIDITT
                     ANVYEHIKILRNETITKNYDIDTKIR"
     gene            complement(4105..4455)
                     /locus_tag="VF222_12510"
     CDS             complement(4105..4455)
                     /locus_tag="VF222_12510"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX7143289.1"
                     /translation="MVLRVHNAYPVEVWVAILYPSTFCSQNEGTSWAKKGWWHLNSNE
                     TKIVLGGDLSPQWMYYLHAHSSDGTEWGNYTYAYCPSQAFDWCSNTSSNTSIKRNFDE
                     VYSNVPNKTINLVG"
BASE COUNT         1511 a          744 c          666 g         1610 t
ORIGIN      
        1 tgattgatta gtaataaaaa ttaaattatc ccaatcatta taacctttaa gaaattgtcc
       61 cggagtattt tttgcgcaat catctattcc acgaaataaa tttatatctt gaggtatgtt
      121 agattcaaaa ttgttattat agttccaatc aattgcacgt ttaacaaaat taaattggtt
      181 accataaata ccgtaaatta aaacatcagg tatctctaat gggatttttg tagtaattcc
      241 ttttgtttca tcaagactag attcattcaa aggattaagt tctattctag aataatccaa
      301 tttataaaaa gctggtggta atattactgg catttgtctg agataattca ttatacttag
      361 ataatttggc ttacaattaa tgccatcacc acccccatga ccaagaccta aattgtgtcc
      421 gaattcatgc attaatgtac cagcttgatg gttagcattt ccattataat gcccaattac
      481 atttgctcca ccagatttcc aatttccaag tgatacaata aaatccatac ccggtattcc
      541 atgggatagt ccagacatag cattgcctac aaaatcaact gcatgagcaa agattacata
      601 atgaaaaatc ttgcttttcg caagtaaagt attgttaaca tttggattat cgtctttctc
      661 atctttggta ccaaaatatt tattttttaa atcatcaaat cctttccagg ttcgtacata
      721 atctgatcct acaaatgaat aggttggaat aactggttga tggggaattt cttgatcata
      781 ttgtatatgt aaatttattc catctggttc ttcacctgga ttacatacgc ccgaatcttt
      841 aaatgcttgt atgatgcgct cacgaactcc ttcaaaaata tcatggtgtt tcatataatc
      901 tatttctaaa aataggtctt tacgtagagg actaacatta taagaagaca aaccataatc
      961 aatgatgcta tcattattta tatcgatacc cctagtttcc caatcgttag gtataccatc
     1021 accatcccaa tcagtttcat tataaataca atctaaatct tgagaatatg actttgaaaa
     1081 agtgattgat attaatatta taacaaatga aattgttata gtactcaata tattgtaata
     1141 cataatttta ttggataata atataaaaat atttaaattt tttcctattc atagtaataa
     1201 aaataaatat ttcattattt gactttatta ttactaactg attacattaa gttatacaac
     1261 actatttgaa ttcaaaattt atagttgtta ttattggttg tatttttatt tcaatacttg
     1321 ttatttataa aatatttaag taattttata ggataattat ctaattattt aaaacataaa
     1381 aaaaggaaag agaatggtaa gttactttta tgattgtccg aatatgaagt agcgaggttc
     1441 aagacaagat cctgttgtac atccttgaag agtagaagct gaaccttcga atagcggtcc
     1501 tacaccagag agaggtctag ttctatcata atctccccat ctatccgctc catttttacc
     1561 agaaactata ttgtatgctg gataaggcgg tggagtacca cttgctgaat taataattcc
     1621 tgctgcaata ctaggataat agctgccacc tccatagtag gaaacaaatc caatatttcc
     1681 gttagaatct ggtgagaaga atggatacat ccatgctata ttggaactcc acaatacggg
     1741 attggattta tatgttaagc cagcttcttg gaatatgaca acatcaacat atggataagt
     1801 gaatgctccg ccctgtttag cattccatgc aaaacctatt tctccattat taatccatcc
     1861 tgttagtatt ctactatcag atcttgcaca ccaatttagt ccatcaggac ctgggcatga
     1921 cattgctccc cattggaatg cgggagtatt tctgtatgca atatttattg tattagtaga
     1981 ttctgcccat ttgtataatt tcatctgagc atttgtatta tgtacagccc aatacattgt
     2041 atctgttgcg ccttgtacag gagtataatt gaattggtta gtttcatagt aatactggaa
     2101 atttactgtc gaaccagaac ttaattgttt cagatcccat tgcgtaatta cagatctaac
     2161 aaaattgtcg tttttatcaa acatatttga ggtgatgtat agtttattgt tagagagcgc
     2221 taaatgagga taatcccacc aactattttt ccagttttga tttactaccg taggatgtat
     2281 actataagac gtccaagttt gagcatcttt ggatatactt aatatgaaac gattttctcc
     2341 atttacatct ttaatgcctt gcctaaacca gatgaagatt ttatagtttt tatcatatat
     2401 tacatcttgg tcacaacaaa aatcagacat accttgcaat gggttaacgt aattccaagt
     2461 gcttccatag tcagccgaac gagcggcaaa ccaatttcca gtataaaata cattaggtcc
     2521 attgtttgca acactaggtt ctgttactgt tgaacgatat tgtgttggtg aaactaatgt
     2581 acttttatat aatgttaagg tggtagcaag agtagcattt ttggttattg tattcgaaga
     2641 tgcattagaa ttactaatat cttctgtgat attactattc aatgcgggtg gagaaactaa
     2701 tacatctgtt gcagtagatt catcaagtcc tgactctgct aaaccttgtg ctccttcgga
     2761 ttctctttga aattgctgtg gatattcacg ttcttcctca gtaatttctt cctcaaaagc
     2821 ttttaattgc tctaacgatg gcgcctgtgg ttcactaaaa ccttcatatt ttgttttctc
     2881 ttcactaggt tgaacagttc cagttccgtt tatttcggca gtaagcattt cactagaggt
     2941 atcattattt tgtgcaaatg cattgtattg aatattcgga aaggcaaata aggaaaatga
     3001 tatcgcaaaa ataacaagag gtaccatttt cagatattta acttgttttc taattgccat
     3061 gagtgcattt acacattaat actcatttat aagacttttt ctcataagaa actaacagaa
     3121 aacttttcta gaataattat caataggtct atccaacaat ttatttataa aaaaaattta
     3181 aatttttcgg ctcctcaaat gcttgatgat aattatatct ccatttcaag atttgcatat
     3241 ctaacgatta aaataaaaaa tttgtcatat ttagtctatt taactacaat agaaatattt
     3301 ataataataa aactccctaa ttaatatata ttgtgaaaaa tacaaaagga tttgttttct
     3361 atagtgtact aatattaagt ctcttactta tttgttatac ttttaatata gatacttatg
     3421 aagaagggat agtgtctaat ggtaatatat acgaaaaaca gaatgaccat aaaaattact
     3481 ctgattctgg tatacaagga acaataaatt ttgttggatc tgactgtccg ccatttagta
     3541 aaaaacaaac tcctccttgt tctggtcctt atcctgatct acctatttca atatataccg
     3601 atgataatga aaaaaagagg atcgcaacca cacacactga ttcaaatggt aattaccata
     3661 tatttcttaa gcctggtaaa tatataatat attcttcatc tgaatcttta ttaccagtaa
     3721 caatagatat tacaacagca aatgtttatg aacacataaa aattctaaga aatgaaacaa
     3781 taacgaagaa ctatgatatc gatacaaaaa ttcggtaaaa ataaatttat tacaatatta
     3841 ttttaataaa tggaaatgat actaaattga atacatgtga tcaattatat tatactaaca
     3901 tgactttttt atttgtgact atattgattt aaataaacaa tattactcgt gttccctggt
     3961 tcaatctatg caaaaaatta gatttggaag agataaaaca tttacaataa tcgtaatata
     4021 ttttctatgt tattactttt ttgtctcaaa ctgcatttta ggtccaggca ttagaccttt
     4081 taataatgaa acgtaaacag acattcatcc aactaagttt attgttttat ttgggacatt
     4141 tgaatatact tcatcaaaat ttctttttat tgaggtattt gatgatgtat tagagcacca
     4201 gtcgaaggct tgactagggc aatatgcgta agtatagtta ccccattcag ttccatctga
     4261 tgaatgtgca tgtaaatagt acatccattg tggacttagg tcacctccta aaacaatttt
     4321 tgtttcgttt gaatttaaat gccaccatcc tttttttgcc cacgatgttc cttcattttg
     4381 gctgcaaaat gtgcttggat agagaattgc cacccatact tctacaggat atgcattatg
     4441 gactctgagt accatttata tttacacctt ctcctgagag ggagtatatt tatgtacaaa
     4501 agagggagga ttttctagag cttcattctt g
//
LOCUS       DASVEV010000286         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_155057, whole genome shotgun sequence.
ACCESSION   DASVEV010000286 DASVEV010000000
VERSION     DASVEV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155057"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VF222_12515"
     CDS             complement(<1..1139)
                     /locus_tag="VF222_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HEX7143290.1"
                     /translation="MVSNFVDIWPIINDILRREGIARQHLNSYNEFMESGLQSIIDEV
                     NEVEIETAEFPYKLKLGKIKLQRPRIMELDGSITHVAPVEARLRNLTYASPIMLECSI
                     VEDGRIIESRFIHVGDMPVMVKSNVCILHNLPESKLIELGEDPRDPGGYFVINGSERV
                     IVGLEDLSYNKIIVDSEETAGILNYKAKVYSSIVGYRAKLELIMKPDGSIVAKIPGSP
                     VDIPLVTLIRALGLESDRDIANAISFKEKIQDELEPSFEKVGEIISSRDAIVYISKRI
                     APGMLEEFQIKRAETLLDWGLLPHLGKNPENRGEKALFLGEAASKLVELKLGWVDNDD
                     KDHYGNKVIKFAGQMLADLFRTAFRNLIRDMKYQLERSGQKRGINA"
     gene            complement(1155..1406)
                     /locus_tag="VF222_12520"
     CDS             complement(1155..1406)
                     /locus_tag="VF222_12520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HEX7143291.1"
                     /translation="MSADKKIVKVNNHIYQPKHEILSKDESEAILKKFNVRTNQLPYI
                     MSSDKGLEELDIRPGDIIKITRKSPTAGESVYYRYVVQG"
     gene            complement(1618..1721)
                     /locus_tag="VF222_12525"
     tRNA            complement(join(1618..1652,1683..1721))
                     /locus_tag="VF222_12525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1684..1686),aa:His,seq:gtg)
     gene            complement(1779..2579)
                     /locus_tag="VF222_12530"
     CDS             complement(1779..2579)
                     /locus_tag="VF222_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX7143292.1"
                     /translation="MKIGLDLAGWKKIPYDSAIHIKGSNIKKVFVSIDVNPFDIFLAK
                     NLDCDCVLTHHPIGKSMISFHKVFDRHIEYMVEKGIKKNLAFSLVKDLKYKTSLKSHS
                     LIYNDVISTAQILKMPLLNIHQPLDEYMRKIILSKLSTSKLKSVSDLVESIEEIPEFN
                     KALTKVRVCNGKSNNKVGQWVLVIAAGSNGGYDIARTYYENNISTVIYLHIDYNELLK
                     VKKDKKCKNLVILGHLAGDSIGMNQICKELEKREINVVKRGLIDDIDY"
BASE COUNT          844 a          487 c          344 g          986 t
ORIGIN      
        1 gcgttaattc ctcttttttg tccagaacgt tcaagttgat acttcatatc tcttatcaaa
       61 tttctaaatg cagttctaaa aagatcagca agcatttgtc ctgcaaattt tataacctta
      121 ttgccataat gatctttatc atcattatca acccatccaa gtttaagttc tactaactta
      181 ctagcagctt ccccaagaaa aagagctttt tcaccacgat tttctggatt ttttcctaaa
      241 tgaggtaaca atccccaatc taataatgtt tcagcacgtt ttatttggaa ttcttcgagc
      301 atacctggag caattctttt actgatataa acaatagcat ctctgcttga aatgatttct
      361 ccaacttttt caaatgaagg ctctaattca tcctgtattt tttctttaaa tgaaatagca
      421 ttggctatat ctctatcaga ttccagtccc aaagcacgga tcaatgttac taagggaata
      481 tcaactggag agccgggtat ctttgctaca atcgaaccat caggtttcat aataagttct
      541 aattttgccc gataaccaac tattgaagaa taaacttttg ctttataatt aagtattcca
      601 gcagtctctt ctgaatcaac aataatttta ttatatgata aatcttctag tcccactatg
      661 actctttcgg atccatttat cacaaaatat ccaccaggat ctcgagggtc ttctcctaat
      721 tctattaatt tagattctgg aagattatga aggatacata cattcgattt taccatgaca
      781 ggcatatcac ctacatgaat gaatctagat tctataatac gaccatcttc tacaatactg
      841 cattcaagca taattgggga tgcatatgta agatttcgta acctcgcctc tacaggagcg
      901 acatgagtaa tagatccatc tagctccata attcggggac gttgaagttt tatttttcct
      961 aatttcaact tatagggaaa ttcagctgtt tctatttcga cttcgtttac ttcatcaatt
     1021 atactttgta atccagactc cataaactca ttgtaggaat tcaaatgctg tcgtgcaata
     1081 ccttctcttc ttaaaatatc attaataata ggccatatat caacaaaatt acttaccaaa
     1141 taacttgtat ctccttatcc ttgtactaca tatctgtaat atacactttc acccgcagtt
     1201 ggacttttcc ttgtgatttt aattatatct cccggacgta tatctaattc ttctaatcct
     1261 ttatcgcttg acattatgta tggtaattga ttcgttctta cgttaaattt tttaaggatg
     1321 gcctcagatt catcttttga aagaatctca tgtttaggtt gatatatgtg attatttact
     1381 ttgacaattt ttttatctgc tgacaaatta ataaccgtcc cccaaattta tcattagata
     1441 aataaatcct gataaaacag cttggcgcaa tacgaaatca gtaactttca tagagaagga
     1501 ggtatatgta gtttcttacc gtaatatcat aacatacgat ttgttaccgt atttggaaaa
     1561 ttcatcaaac ttatttcaga attactatta ataaattaaa gttgtgatga tcatttaggc
     1621 cttcggaggg atttgaaccc tcctcaagcg gtttctatct ttcttgtttt ataataaaaa
     1681 gaccacagcc gcctatacta accaggctat actacgaagg ccactatggt tttataaaaa
     1741 tctgtattat ttattcttta gagaaatgaa aaataaaatt aataatcaat atcatcaatt
     1801 aatcctcttt taactacatt tatttctctt ttttctagct ctttacatat ttgattcatt
     1861 cctatggaat ctcctgctaa atgtccaaga ataacaagat tcttacactt cttgtctttt
     1921 tttactttta aaagttcatt ataatcaata tggagatata tgacagtaga aatattattt
     1981 tcataataag tacgtgcaat atcatatccg ccatttgatc cggctgctat aacaagaacc
     2041 cactgaccta ctttattatt tgattttcca ttgcaaactc tgacttttgt taaagctttg
     2101 ttaaattctg gtatctcttc tattgattca actaaatctg aaactgactt taatttagaa
     2161 gtagaaagtt tagataaaat aatttttctc atatattcat caagtggttg gtgaatattc
     2221 aataatggca ttttgagaat ttgtgcagtg ctaatcacgt cattatagat taaactatga
     2281 gattttaaag aagtcttata ttttaaatct ttaactaaag aaaacgctaa attttttttg
     2341 atccccttct cgaccatata ttctatatgt ctatcaaaaa ctttgtgaaa tgatatcatt
     2401 gactttccta tggggtgatg tgttaatacg caatcacaat ctaaattttt tgcaagaaaa
     2461 atatcaaagg ggtttacatc tatggaaaca aaaacctttt tgatattgct accttttatg
     2521 tgaatcgcac tatcgtatgg tatttttttc catccagcta aatctagtcc aattttcatc
     2581 aattcacctg tatccaatcc tgtcaattat tataaaatta taattattgg gaaatattta
     2641 cttttaaaca aatctctaaa t
//
LOCUS       DASVEV010000287        11379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_932364, whole genome shotgun sequence.
ACCESSION   DASVEV010000287 DASVEV010000000
VERSION     DASVEV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932364"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF222_12535"
     CDS             <1..327
                     /locus_tag="VF222_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143293.1"
                     /translation="EQDLIIEELCGRDRGKRTYTVTPSKITSIVNLNKIIHNAELLGY
                     NIKTRGSLGITVTTNNSNPSQQQQQQLSISFMTSGTATIVGAKDEKDALSIYYEFLNS
                     KISNKK"
     gene            complement(336..995)
                     /gene="psmB"
                     /locus_tag="VF222_12540"
     CDS             complement(336..995)
                     /gene="psmB"
                     /locus_tag="VF222_12540"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HEX7143294.1"
                     /translation="MHVQDNRYSEYIANQIKKGTTTCALSCSDGVVLAADTRASAGMF
                     IADRRVMKIQKVDNHLGMTIAGGVADAQNLVDTMRYNANLYRINNKRIIPVQSAARLC
                     SNILFNQRYFPYYVQIIMAGYEGKGAGQIYNIDLFGSLTEEKFISTGSGSPVAYGYLE
                     GEFKENMSVNEAYKVALQAIAAAIRRNAGTGDGINVVIIDKNGYNELSKEVKDAVKVV
                     F"
     gene            1092..2531
                     /gene="leuC"
                     /locus_tag="VF222_12545"
     CDS             1092..2531
                     /gene="leuC"
                     /locus_tag="VF222_12545"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7143295.1"
                     /translation="MSYTLFEKIWDNHIVYPNSKSINQSINHDSYLLYIDRHLIHEVT
                     SPQAFDGLRTNNRKVRRPDLTFATMDHNVPTTNRSLPIVDQVSAIQINTLVENCKEFG
                     IRLFDLDSPNQGIVHVIGPELGITLPGATIVCGDSHTSTHGAFGAFAFGIGTSEVEHV
                     LATQCLWLTKPKTMEVNFFGKPKNSLAVSAKDIILALIRKIGIGGGIGAVVEYRGDMI
                     KKLSMEKRMTICNMSIEAGARAGLIAPDEITFEYIRNREYSPEGEKFEKMVTYWKEDL
                     KTDNNASFDKKVEVNISNLSPQVSWGTNPSMVVDVTGIIPYADEYAMGNEDEKKAATR
                     ALEYMNLKSGTPITDISIDRVFIGSCTNSRLEDLIEASVIVKGRKVSPKVHAMVVPGS
                     QSVKLKAEKLGLDKIFIESGFEWRESGCSMCLGMNPDILSSGERCASTSNRNFEGRQG
                     VGGRTHLVSPVMAAAAAIEGHFVDVREWI"
     gene            2512..3183
                     /gene="leuD"
                     /locus_tag="VF222_12550"
     CDS             2512..3183
                     /gene="leuD"
                     /locus_tag="VF222_12550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7143296.1"
                     /translation="MFENGYKYSTHPLNLIIMEPFQNFESKATPFSYVNVDTDQIIPK
                     QFLKLTQKTGFGKYLFYDWRYNKNGEPKKDFILNNPTYSGYPILLTLDNFGSGSSREH
                     AVWALMDYGFRVIISPSFADIFYSNCIKNGILPIILPKKEIEYLFDNPDCIIKVDLNK
                     QQIELKPISRQLKFQIDPSKKKTLLEGLDEIGMTLQLESHISDYENKVHKDFNINVLS
                     KSKLI"
     gene            complement(3190..4782)
                     /gene="tgtA"
                     /locus_tag="VF222_12555"
     CDS             complement(3190..4782)
                     /gene="tgtA"
                     /locus_tag="VF222_12555"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HEX7143297.1"
                     /translation="MSFEVRHSDLGGRIGKLNTAHGIIDTPAFIPVVHPIKQMVDVKL
                     LKKMGFKAIITNAYIALKHYGDNARKKGIHRIIDFDGIVMTDSGGYQVLEYGNIDIGP
                     VEMAKFEIDIKTDIAVPLDKPTGFGLEYSKAIKYVNQTISNVKETVEVIKNNSCNTNE
                     INTIWAGPVQGAEHLDLVEQSAKLFDEMNFKLMALGSPVELMEAYEFAKLSEIICTLK
                     KTIPSKPIHLFGAGHPLTIPLAVALGCDMFDSASYILYAKDNRYMHSNGTMRLEEITY
                     FPCSCSICNSYTPHELLETTEIDRIIQLSIHNLYVLKKEVNSVKQSILDGRLWEYIMS
                     KAHNHPKLMEAVRLFKNFDYLEEGTPIYKDKAIYFNEPYDQYRPEAKRFRKMVTKYII
                     NNKNKLILYPETKIHPFYASKNFIKIKEIFSNVEVFSYNPFLGIIPAEISDIFPASHN
                     IIAAEDDSQSFAANYPTFIDAMKFFLKVNQGKNIIIIANEFMKQIIYYFYSQENIIET
                     FNSNIKIVDYKENILFEISEIQ"
     gene            complement(4785..5195)
                     /locus_tag="VF222_12560"
     CDS             complement(4785..5195)
                     /locus_tag="VF222_12560"
                     /inference="COORDINATES: protein motif:HMM:NF026244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsm family RNA-binding protein"
                     /protein_id="HEX7143298.1"
                     /translation="MSVAFRKFGEESIQFLGKKVSIETSDKKTYGGTLIGINEKLDVI
                     LVDLETSGIIKIIINGSFIKEIRLMEKPFDLKALAERFSRVFPGLVKVREDVGAIIVM
                     DKIKVTQNGIEEGTGLAADRIKAIYDEFVRETKK"
     gene            complement(5288..5851)
                     /locus_tag="VF222_12565"
     CDS             complement(5288..5851)
                     /locus_tag="VF222_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7143299.1"
                     /translation="MIRIGITGNPGVGKHTITDLLSKKIKNSKIIDINKIIISNQAFN
                     YGTSEADLKKTRLFLLKELIDDNVIVVGHLLPYVIKRNELDFIAILRKSPYSLIDVYE
                     KRKYSNKKSQENILCEILGICFYDSLKVFGKRKISEIDTTFKNPEESVKKIIYNYDHK
                     SKRQIGLVDWLDVIYKNGDNRKFLYVN"
     gene            complement(5969..6262)
                     /locus_tag="VF222_12570"
     CDS             complement(5969..6262)
                     /locus_tag="VF222_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7143300.1"
                     /translation="MTIAILPDVDEQRCIGCALCVEICTALGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACVGVCPTHAIFWMRPMEYTPGQPVPLHKNGVFDKGWEEG"
     gene            complement(6413..6634)
                     /locus_tag="VF222_12575"
     CDS             complement(6413..6634)
                     /locus_tag="VF222_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143301.1"
                     /translation="MGRSFTDWWNTVPADLKEKARRGDENNKPLLNQVNYVLLHLHLA
                     GKHDAKPSHEELKDWLHSGQVDVLRMIKK"
     gene            6789..7640
                     /locus_tag="VF222_12580"
     CDS             6789..7640
                     /locus_tag="VF222_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143302.1"
                     /translation="MSFSFEEILKAYEFYKQYTKLQKPSSCYHERFIFPLFSGTYFCY
                     RKIDVLRVISIAKALSANAEYIDIGCGNGDFLRKIRKYLPHSIGIEQNTLPLYLLKNT
                     KPDYIYSIPIELFTNKYTFDLAFVGWMQPGVDFRKYVAKLANCIITTFDSGGQCGING
                     GCEYEEFGFQKIASWRTPSWIDVNSELMNNYYTGSLTNKANLRQHLSKLRTANNFWYV
                     YAINSMSNKITTALKSQIQKEENDHLFSREKFDFEAVLDECGFGYYEKLSSSLSTEKH
                     LWKVNFH"
     gene            7708..8037
                     /locus_tag="VF222_12585"
     CDS             7708..8037
                     /locus_tag="VF222_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143303.1"
                     /translation="MKLRDEEYSYSSISGKLATIFLFYDMNDIVLNKKKICRYMGNMK
                     RQLAKIKGYQGIDDYVLNLIEHKLESIRDGGQGIDDLGEYVIEYLAKIIGPDDEEEKE
                     VKDEEEV"
     gene            8275..9243
                     /locus_tag="VF222_12590"
     CDS             8275..9243
                     /locus_tag="VF222_12590"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7143304.1"
                     /translation="MKKDKNKDKENHEGDVSSNTTTKRKREKIQQKEGRSIAIESNNQ
                     EEEEDIKEKIIDRMVDRNYNTYNKTEHGTKQNSVLPSTENRTIYDLQTLLSQRQDRLE
                     SVFYERYQYNTSIKRGQDFLLNHVDSKLSLVVMYADLVGSTKMSMALPVERMAKIIKA
                     FSHELSSVVESYDGFVLKYVGDAVIAFFPSGFNKYLSCDTAFQCAKSMIDVIENGINP
                     ILEKDKDNYPKLAVKIGIDEGENLAIQYGYDKSAPIDLIGYPMNVAAKMTSLTGPNKI
                     TVGNNVYKLLHPTLQPEFQEMQMKEGTEWKYIDLETNLLYKVYTMK"
     gene            complement(9507..9830)
                     /locus_tag="VF222_12595"
     CDS             complement(9507..9830)
                     /locus_tag="VF222_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143305.1"
                     /translation="MFNNFNSFNTSYDLIPKQLLYYIDYELSCFEKITNKKFFFKVLK
                     NDKFELNVYESKEEEEKEEDDNKSKYQIIYRANNLYQFIKRLLMIREYLIKYFDNKLT
                     KTIIL"
     gene            complement(10091..10264)
                     /locus_tag="VF222_12600"
     CDS             complement(10091..10264)
                     /locus_tag="VF222_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7143306.1"
                     /translation="MMILNLKSAFLISKHVTRQMISSKYVKIIHVSSKNGLQSEGYDS
                     AYSVSKSGLINFV"
     gene            10495..10953
                     /locus_tag="VF222_12605"
     CDS             10495..10953
                     /locus_tag="VF222_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7143307.1"
                     /translation="MTEMSKVEICRFLMHGTFTGKLATVKKDGSSHVVPIWFVLENWN
                     SRRSRVRNIIFTTGSTSVKANNIQRDSRVSICIDDQIPPFSFVTIFGTAKILSYKQKE
                     VLKWATKIAERYMGKSDAEAYGRRNSEEGLVLVRIKPTRIIAEKDIADWD"
     gene            complement(11150..>11379)
                     /locus_tag="VF222_12610"
     CDS             complement(11150..>11379)
                     /locus_tag="VF222_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX7143308.1"
                     /translation="HNIKGNTKGCEECEKIGSDWVHLRLCLSCGHVGCCDSSVNKHGT
                     KHYQETDHPIIKSYEPGENWKWCYKDNLFME"
BASE COUNT         3974 a         1714 c         1655 g         4036 t
ORIGIN      
        1 gaacaagatc ttattattga agaactatgt ggtagagata gaggcaaaag aacttatact
       61 gtgacaccat caaaaataac ttctatagtt aatttaaata aaataataca caatgcagaa
      121 ttattaggat ataatataaa aactagaggg tcgttgggaa taactgtaac gactaataat
      181 agtaatccat cacaacaaca acaacaacaa ctatctataa gttttatgac tagtggcaca
      241 gcaactatag tgggagcaaa agacgagaaa gatgcgcttt ctatttatta cgaattttta
      301 aattctaaaa ttagtaataa aaaataaatt tatttttaga atactacttt aacagcatct
      361 tttacttctt tcgaaagttc gttatagccg tttttatcta ttataacaac gtttattcca
      421 tctcctgtac cagcatttct tctaattgct gcagctattg cctgcaaagc tactttatat
      481 gcttcattta cactcatatt ttctttaaat tcaccttcta aataaccata agctacagga
      541 gatccactac ctgtagaaat aaatttttct tcagttaatg atccaaacaa atcaatatta
      601 taaatctgac cagcaccctt accttcgtat cctgccatga taatctgaac ataatatgga
      661 aaatatcttt gattaaacaa aatattagaa cataatctgg cagctgattg aacaggaata
      721 attcttttgt tatttattct atatagatta gcattatatc gcattgtatc tacaagattc
      781 tgagcatcag ctacgcctcc tgcaatagtc attcctaaat gattatcaac tttttgaatt
      841 ttcattaccc gtctatcagc gataaacata cctgcactag cacgggtatc agcagctaat
      901 acaacaccat cagaacatga aagagcacaa gtagttgttc cttttttaat ctgatttgct
      961 atatactcag aatatctatt atcttgtacg tgcattttaa tcaaagataa tcagttcaat
     1021 actacataag tattttttca ttctttagga tttatatgat tatattttac tagtatgtaa
     1081 tatataatat aatgtcatat actctcttcg aaaaaatttg ggataatcat atagtatatc
     1141 ctaattctaa aagtattaac caatcaataa atcatgattc atacttactt tatatagatc
     1201 ggcacttgat ccatgaagtt acttcaccac aagcgtttga tggtctaaga acaaataata
     1261 gaaaagttag aagacccgat ttgacttttg ctacgatgga ccataatgtt cccaccacaa
     1321 acagaagtct tccaatagtt gatcaagtct ctgctataca aattaataca ttagtagaaa
     1381 actgtaaaga atttggtata cgtttatttg atttggatag tcctaatcaa ggtattgttc
     1441 atgttatcgg tcctgaacta ggaataactc ttccaggcgc tactatagtt tgtggagata
     1501 gtcatacttc tactcacgga gcttttggag cctttgcttt cggaattggt actagtgaag
     1561 tagaacatgt acttgcaact caatgcctct ggctaactaa accaaaaacc atggaagtaa
     1621 atttttttgg taaaccaaaa aattcactgg cagtatcagc taaagatatc atattagcac
     1681 tcattcgcaa aataggtata ggtggaggta ttggagcagt agttgaatac cgcggtgata
     1741 tgataaagaa actgtcaatg gaaaaaagga tgaccatttg taatatgtca atagaagccg
     1801 gagctagagc cggattaatt gctcctgatg aaataacctt tgaatatata cgtaatcgag
     1861 aatattctcc tgagggagaa aagtttgaaa aaatggttac atattggaaa gaggatttga
     1921 aaactgataa taatgctagt tttgataaaa aagtagaagt aaatatatca aatctttctc
     1981 cacaagtaag ttggggaaca aatccttcaa tggttgttga tgtaactggt ataattccat
     2041 atgctgatga atacgccatg ggtaatgaag atgaaaaaaa ggcggcaact cgagcactgg
     2101 aatatatgaa tttaaaatca ggtactccaa ttactgatat cagtattgac cgagtgttta
     2161 taggttcatg tacaaattct agattagaag atctaattga agcatctgtt attgtaaaag
     2221 gaagaaaagt ttcaccaaaa gttcatgcta tggttgtacc aggatctcaa tcagtcaagt
     2281 taaaagcaga aaaattagga ttagataaaa tctttataga gagtggattt gaatggagag
     2341 aatctggatg cagtatgtgt ttgggaatga atcctgatat tctttcctct ggtgaaaggt
     2401 gtgctagtac atcgaatcga aactttgaag gaaggcaagg agttggaggt agaactcact
     2461 tggtaagtcc agttatggca gcagcagcag caatagaagg acattttgtt gatgttcgag
     2521 aatggatata aatattcaac acatcctcta aatctaatta ttatggaacc atttcaaaat
     2581 tttgaaagta aagcaacacc tttttcatat gttaatgttg atacagatca gataatccca
     2641 aaacaatttc ttaaattgac acaaaaaacc ggatttggaa aatatttatt ttatgattgg
     2701 cgttacaata agaatggtga gcctaaaaag gacttcatat taaacaatcc gacatattca
     2761 ggatatccta ttcttcttac attagataat tttggaagtg gaagctctag agaacatgca
     2821 gtatgggcac taatggatta tggttttaga gttattattt cgccttcatt tgccgatatt
     2881 ttctatagta actgtataaa gaacggtata ttgccaataa tattgccgaa aaaagagata
     2941 gaatatcttt ttgataatcc tgattgtatt attaaagtag atttaaataa acaacagata
     3001 gaattaaaac caatttcaag acaattaaaa tttcaaatag atccgtctaa gaaaaagacg
     3061 ttattagaag gtttagatga aattggaatg acattgcaac tagaatccca tatttcagat
     3121 tatgaaaata aagtacataa agattttaat ataaatgtcc tatcaaagag taaattaatt
     3181 taaaagatct tattgtatct cggatatttc aaataagata ttttctttgt aatctactat
     3241 ttttatattt gagttaaaag tttcaataat attttcttgg ctataaaaat aataaataat
     3301 ttgtttcata aattcatttg ctataattat tatattttta ccctggttaa cttttaaaaa
     3361 aaacttcatt gcatcaatga atgtcggata atttgctgca aaactttgag aatcatcttc
     3421 tgccgctatg atattgtgtg atgcgggaaa tatatctgat atctcagctg gaataattcc
     3481 taaaaatgga ttatatgaaa aaacttccac attagagaat atttctttga ttttgataaa
     3541 atttttcgag gcataaaatg gatgtatttt tgtttcagga tataaaatta gtttattttt
     3601 attgttaata atatattttg ttaccatttt tctaaatctt tttgcttctg gtcgatattg
     3661 atcatatggc tcattaaaat aaatggcttt atctttataa attggtgtac cctcttctag
     3721 ataatcaaag tttttaaata acctaacagc ctccattaat tttggatgat tgtgagcttt
     3781 gctcattata tactcccaga gccttccatc cagtatcgat tgttttaccg aatttacttc
     3841 tttttttaag acatataaat tatgtatgga taattgaata attcgatcaa tttcagtggt
     3901 ttccaataac tcatgtggtg tataagaatt acaaatcgag caagagcagg ggaaatatgt
     3961 aatttcttcc aatctcatag ttccatttga atgcatatat ctattatctt tagcatataa
     4021 aatatatgat gctgaatcaa acatatcaca tcctaacgca actgctaatg gaatagttaa
     4081 tggatgccct gctccaaaaa gatgaatagg cttagacggg attgtttttt ttaaagtaca
     4141 aattatttca gaaagtttag caaattcata tgcttccatc aactcaactg ggcttcctaa
     4201 agccattaat ttaaaattca tctcatcaaa gagttttgca gattgttcta ctaaatctaa
     4261 gtgttcagca ccttgaacag ggcctgccca tatggtattt atttcattgg tattacaact
     4321 gttattttta attacttcaa cagtttcttt aacattacta attgtttgat taacatattt
     4381 aattgcttta ctatattcaa gtccgaaacc cgttggttta tctaaaggaa ccgctatgtc
     4441 tgtttttatg tctatttcaa attttgccat ttcaacaggt cctatatcaa tatttccata
     4501 ttctagcact tgataacctc cagaatctgt catcacaata ccatcaaaat caattattct
     4561 atgaattcct ttcttccgag cgttatctcc atagtgtttt agagcaatat atgcattagt
     4621 aataattgct ttaaatccca tttttttgag caattttaca tctaccattt gttttatggg
     4681 atgaacaact ggaataaatg ctggcgtatc tatgattcca tgtgctgtat ttaattttcc
     4741 tattcgtccg cctaggtcac tatgtctgac ctcaaacgac aatgttattt ctttgtctcc
     4801 cttacaaatt catcataaat tgcttttatt ctatcggctg cgagtcctgt accctcttca
     4861 atgccattct gagtaacttt aattttatcc ataactataa tagctccaac atcttctcta
     4921 acttttacta aacctggaaa aacccttgaa aatcgctctg caagagcttt taaatcaaat
     4981 ggtttttcca ttaatctaat ttccttaata aatgaaccat ttatgattat ttttattatt
     5041 ccactagttt ctaaatctac taatattaca tctaattttt catttattcc aattaatgtt
     5101 cctccatatg tttttttatc tgatgtttct atagatactt tctttcctaa aaattgaatt
     5161 gattcctctc caaattttct aaatgctaca gacaagctaa ttcgattgtg gttataatag
     5221 cagctatata taatttcaat atctaccact tttttccttt atcataattt aacaattttt
     5281 tttatattta gttaacatac aaaaattttc tattatctcc atttttatat attacatcta
     5341 accaatcaac taatcctatt tgtctctttg atttgtgatc ataattataa ataattttct
     5401 tcactgattc ttccggattt ttgaaagttg tatctatttc tgaaattttc cttttaccaa
     5461 acacctttaa actatcgtaa aaacatattc ctaaaatttc acagaggata ttttcttgcg
     5521 actttttatt ggaatatttt cttttctcat acacatctat caaagaatag ggagattttc
     5581 ttagtatagc tataaaatct aattcatttc tttttattac gtatggtaaa agatgtccaa
     5641 ctactattac attatcatca atcaattctt ttaataagaa taatctagtt ttttttaaat
     5701 cagcttctga tgttccataa ttgaaagctt gatttgatat tattattttg tttatatcga
     5761 ttattttact attttttatt tttttagata gcaaatcggt tattgtatgt ttaccaacac
     5821 caggattacc agtaattcct attcttatca tactatctaa taaaatataa tcttaatcca
     5881 aatctaaata cagatagata aaaaaatcta caacaaaaat ataaaaaatg aattgaaatt
     5941 ttctattcga ttgtagtgta tggtgttttt aaccttcttc ccagcccttg tcaaatacac
     6001 cgtttttgtg taatgggact ggttgtcctg gtgtgtattc cattggtctc atccagaata
     6061 ttgcatgtgt tggacaaact cctacgcaag caccatcaga aatgcatctt tctggataaa
     6121 atacaaaggc tttacctctt ttccaacctt ctacaggttt tactctaaga acgtctggac
     6181 caagagcagt acaaatttct acacaaagtg cacatccaat acatctttgt tcatcaacat
     6241 ctggaagtat tgcaattgtc attcaatatt tcactaatgg tccgatgttg gcgaaactat
     6301 ttaaacccta tgtctatatc aaaacagcgt tataatcatt tttttatggt taaataagga
     6361 gaatattata taaaaatttt aaataataaa aaaacgaaag tataatttta atttattttt
     6421 taatcattct caatacgtct acttgtccac tatggagcca atcttttagt tcttcatgtg
     6481 atggttttgc atcatgtttt cctgctaagt gcaaatgaag aagaacataa ttaacttggt
     6541 tcaataatgg tttattattt tcgtctcctc tacgtgcttt ttcttttaaa tctgctggaa
     6601 cagtattcca ccagtcagta aaacttctac ccacattaag agttaataat atagattaat
     6661 aagccttttg ggatttttta acagcgttat aaaaaatttt tctgttctat aagtgatctt
     6721 caactaattt taaacaataa tccaaattaa ttataaatat attctcagta tttattattt
     6781 gttaatctat gtcgttttca tttgaggaga ttcttaaagc atatgaattt tacaaacaat
     6841 atacaaaact acaaaaacca tcttcttgtt atcatgaacg atttatattc ccattatttt
     6901 ctggaaccta tttttgttat agaaaaattg atgttttacg tgtaatatca attgccaaag
     6961 ctctttctgc taatgctgaa tatattgata ttggatgtgg taatggagat ttcctacgaa
     7021 aaataagaaa atatctacca cattctatag gtatagaaca aaatacattg cctctttatt
     7081 tattaaaaaa tactaaacct gattacatat attcaattcc cattgaattg ttcacaaata
     7141 aatacacatt tgatttagcc tttgttggat ggatgcaacc gggtgttgat tttcgtaaat
     7201 atgttgctaa attggcaaat tgtataataa ctacatttga ctctggaggt caatgtggta
     7261 taaatggagg ttgtgagtat gaagaatttg gtttccaaaa aattgcttct tggagaactc
     7321 catcatggat cgacgtaaat agtgaactta tgaacaatta ctataccggt tcattaacaa
     7381 ataaagcaaa tttaaggcag catttatcga aattaaggac agctaataat ttctggtatg
     7441 tctatgctat taatagtatg tccaataaga tcactactgc tttaaaatcg caaattcaaa
     7501 aagaagaaaa cgatcattta ttttccagag aaaaatttga tttcgaagct gttttagatg
     7561 aatgtggttt tggctactat gagaaattgt catcttcact ttctacagaa aaacatttat
     7621 ggaaagtaaa ttttcattaa gtatataata aatggccttt tacaaaagga tgtaagaatc
     7681 atacaaagtg atataatcga atatataatg aaattgcgag atgaagaata ttcttactca
     7741 tcgataagcg gtaagttggc tacaattttc ttattctatg atatgaacga tattgtcctt
     7801 aacaagaaga aaatatgtag atatatgggg aacatgaaaa gacaacttgc caagatcaag
     7861 ggataccaag gcattgatga ttatgtacta aatttgatag aacataaact agaatccata
     7921 agagatggag gtcaaggaat agatgatctt ggtgaatatg ttattgaata cctggcgaaa
     7981 ataataggtc cagacgatga agaagaaaaa gaggtaaagg acgaggagga ggtctagtaa
     8041 tagagatgga tagacgtcat agaaataagg caaatttgtt tagatataat tttgatataa
     8101 aaatcgctat tatgccatga tgttataagc tctttttttg aaaccccttt taaaatattt
     8161 taaatattaa atttatataa attaaatttt tgttttttct ctaattgttg cattctttca
     8221 ctctcatcta aatagttaaa ttaaataatt aactgattta attaaggtct tttaatgaag
     8281 aaagataaaa ataaagataa agaaaatcat gaaggagatg ttagttctaa tactaccacc
     8341 aaacgtaaac gtgaaaagat acaacaaaaa gaaggaagat caatagctat agagtctaat
     8401 aatcaagaag aggaagaaga tattaaagaa aaaataatag acagaatggt tgatagaaat
     8461 tataatacat ataacaaaac agagcatggt acaaagcaaa actccgtctt accatctact
     8521 gaaaatagaa ctatctatga tttacaaaca ttactatcac aaagacaaga caggcttgag
     8581 agtgtcttct atgagcgtta tcaatacaat acatcaataa agagaggaca agattttctt
     8641 ctcaatcacg ttgattctaa actttctctt gttgtaatgt atgccgattt agtaggttca
     8701 acaaagatga gtatggcttt accagtcgaa agaatggcaa agataatcaa agccttttca
     8761 catgaactgt cttccgttgt tgaaagttat gatggattcg tattaaaata cgttggagat
     8821 gctgttattg cattctttcc aagcggtttt aataagtatc tatcttgtga tactgcattc
     8881 caatgtgcta aatcaatgat tgacgtaata gaaaatggta ttaatccaat tttagaaaaa
     8941 gataaagaca attatccaaa attagctgtt aaaataggaa tagatgaggg agaaaattta
     9001 gctatacaat atggatatga taaaagtgca cctattgatt tgataggata tcctatgaat
     9061 gtggccgcaa agatgacttc tctaacagga ccaaacaaaa taactgttgg aaataatgta
     9121 tataaattgt tacatcctac tctacaacca gaatttcagg aaatgcagat gaaagaagga
     9181 actgaatgga aatatattga tctggaaact aatttgctct acaaagttta tacaatgaaa
     9241 taatattcaa aaaatctata gaataaaata aataaaataa tatattatca ttctgttctt
     9301 ataaaaatta tatatatggt gttaactgct ttgctaaatg ataaataata gtttcattag
     9361 atatttttga tctacatcac caagatcatt gcagagaaaa ctttcaatca agtagaaaaa
     9421 atcgtcttta atcatttgtc atagtctgga cggttcccca aacctctggt tcagtctctg
     9481 acaattaatt caaaaattaa tttaaattat aatattattg tttttgttag cttattatca
     9541 aaatatttaa ttaaatattc tctaatcatc aataatcttt tgataaattg atagagattg
     9601 tttgctctat agattatctg atattttgat ttattatcat cttcttcttt ttcttcttcc
     9661 tcttttgatt catatacatt taattcaaat ttgtcatttt ttaatacttt aaagaaaaat
     9721 ttcttgtttg taattttttc aaaacaactt aattcgtagt ctatataata gagtaattgt
     9781 tttggtatta gatcataact agtattaaaa ctattaaaat tgttaaacat cgaccttttt
     9841 tatagtatct ttttattatt taacacttta attcattact tactgttgaa tcatatagtg
     9901 cataaatttc ttttcatttg gttaatatga gtttttatga tatgagaaaa gtatttcttg
     9961 cagtaaaagt aaaatttaat ttagatatta cgaatttgac aattttatat agtaatttaa
    10021 tttaatcaat gagtatatat ttcttatacc aattttttaa gcaagaacgt ttgtatttac
    10081 gtgtggtcat ctaaacaaag tttatcagtc cggatttaga tactgaataa gcagaatcat
    10141 agccttcaga ttgtaatcca ttcttagaag atacatgtat tattttcaca tattttgaag
    10201 agatcatttg tctagtaaca tgtttactta tcaaaaatgc tgattttaga tttaatatca
    10261 tcatcaaatt ctattctttt tcctcaagct cagctacagt cttccctcct agataatctc
    10321 caactacatt aaccaatatg tctatacgac catatttgtc taagatatta gaaactgcgc
    10381 tcctagcttg acaagttctt cagctgcatt ttacgattgt tatctccaaa cattccggaa
    10441 ttaggatatt aaagacacgt attatattca atgaacaagc taagtaataa tataatgacg
    10501 gaaatgtcca aagtagaaat ttgcagattt cttatgcatg gtaccttcac aggaaaactt
    10561 gctacagtta agaaagatgg gagttctcat gttgttccaa tttggtttgt gttagagaat
    10621 tggaatagta gacgtagtag agtaagaaat atcattttta caactggtag cacatcagtc
    10681 aaagcaaaca atattcagcg agatagtaga gtaagtattt gcatagacga tcagatccca
    10741 ccattttcct ttgtgacaat atttggcact gcaaaaatac tctcttataa acaaaaagag
    10801 gttttaaaat gggccactaa aatagcagaa cgctatatgg gaaagagcga tgcagaagcg
    10861 tatggtagaa gaaatagtga agaaggtcta gttcttgtac gaataaaacc tacaagaata
    10921 attgcagaaa aagatatagc cgattgggat tgatgatgat tatttattat tagagccact
    10981 gccacatctt atgcttctta tttttaattg ttggaattat ttccttattg ataaattcct
    11041 cgaaggcagg atcaaaccat ttttaaccat tggcttatta ttagatttta tattattatt
    11101 atccaaacta gaattacttc tttatttaac aatactgtat ttctttattt tattccataa
    11161 agagattatc tttataacac catttccaat tttcaccggg ttcatatgat tttataattg
    11221 gatgatctgt ttcttgataa tgttttgtgc catgtttatt gacagaagaa tcacaacaac
    11281 caacatgtcc acatgaaaga caaagacgta aatgaaccca atctgaacca attttttcac
    11341 attcttcaca accttttgta tttcctttta tattatgat
//
LOCUS       DASVEV010000288         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1710805, whole genome shotgun sequence.
ACCESSION   DASVEV010000288 DASVEV010000000
VERSION     DASVEV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710805"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VF222_12615"
     CDS             complement(<1..1086)
                     /locus_tag="VF222_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7143309.1"
                     /translation="MLEQYNKKRDFGRTPEPQGNKKGLTGNKSSKFVVQKHDASRLHY
                     DFRLEDTKEKVLKSWAVPKGISLNPKIKRLAVLTEDHPLDYLLFEGVIPQGSYGAGTV
                     IVWDTGTYTSEQEISDQFQKGKITFTLFGHKLRGKFTLVRTHLDKGKEGKQWLLIKSA
                     DEFESNEDLTITMPNSVLTRGINKDSEKEQEEDIRYHKKDKTKISFTKAIKLSNNQPT
                     DNTFSNQQQEEVEEFPPTIKPMLSTLVDEAFNSKDWVFEVKWDGVRSILFFNRLNKTL
                     ELQSRNGRFITHRYPELVMTLKSEQITSSSQSAIKCKESAILDGEIVVLDKKTGIPSF
                     QNHQRRMNIDSIREIEIFSRQLPATYYF"
     gene            complement(1096..1287)
                     /locus_tag="VF222_12620"
     CDS             complement(1096..1287)
                     /locus_tag="VF222_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143310.1"
                     /translation="MSDYEWDLKVEVIEPIGKRRMKKHTENNFRLSDEKLKNVVTFGM
                     ILLTEYIINVVSLLLYIVN"
     gene            complement(1367..1666)
                     /locus_tag="VF222_12625"
     CDS             complement(1367..1666)
                     /locus_tag="VF222_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143311.1"
                     /translation="MQLLENRIIELSSSSSSSSSSSSSSSPTLSTTNESTDKEIQQKQ
                     PQLIIDVFGQESEPICTYYRCRHKFSLHGLSTHGCRCKHPMNKTLGVLMRYDEGK"
     gene            complement(1653..1865)
                     /locus_tag="VF222_12630"
     CDS             complement(1653..1865)
                     /locus_tag="VF222_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143312.1"
                     /translation="MLSQVEKTIAAAIESELEIGTTAPNKCLRNSMNFRNQECKSYEN
                     GAESIYIKSDLIYSYQGMANRRNAAA"
     gene            complement(1882..2724)
                     /locus_tag="VF222_12635"
     CDS             complement(1882..2724)
                     /locus_tag="VF222_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7143313.1"
                     /translation="MAARTIWKGSISFGLVNIPVQVFVATQKDEFSSFNQLDEKGHKI
                     KYKKWCPVEEREVPWSEIKKGYEITKNNYVILEKEDLENIKLKTTHTIEINEFIDSKD
                     FDPLFIEKNYYIGPDAGITKKKKNTSPSSSKAYSLFVKILNETNKVAIGKVVLRDKEH
                     LVALRSYQRGLVMHQLKYLDEIKPMDEVEGISSSQQPKVEDKELSLGKTLVDNLTSEQ
                     FDIGKYSDTYSKELEKLIEAKSKGQKVTIEAEEEKAEDTTNLLEALKASINTKNKPRR
                     QKRN"
     gene            3424..3633
                     /locus_tag="VF222_12640"
     CDS             3424..3633
                     /locus_tag="VF222_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143314.1"
                     /translation="MGREYRDKLSPYVRGFESDSPESLGEKITTFLTENPELELKDVK
                     YSSYGFIHNLSRETSYSALVRCNRK"
     gene            4237..5121
                     /locus_tag="VF222_12645"
     CDS             4237..5121
                     /locus_tag="VF222_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143315.1"
                     /translation="MYLYSSISITIRRKEMGIIIRKNDEFTTLLQNKLKERYEQRRRL
                     DNKVHVSDIIPTSCIRKQYYSRKYPELDVISNETVQHFVRGEASELVITSLADLGVSQ
                     SELEMDGLTGRPDIMNRNIIVELKDTMNPQRLNVTDDKFKSYLRQLLYYLVITGIEKG
                     IISIKYNIKELKWVKSDSHGDYFFRPFNSKSPEIESWEIFLPGNDITREILKNEMIRR
                     KNLFLKALENEDVTILPRLSEKIRDRKCPWCKFYEICMDSKNDETEEANEMANEIDIF
                     DIPGVIDLKINKTTTSIT"
     gene            complement(5280..5729)
                     /locus_tag="VF222_12650"
     CDS             complement(5280..5729)
                     /locus_tag="VF222_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143316.1"
                     /translation="MNKNLLIAGIFGFLASAVIAELYALYSPSDVLTSTITVLVGFII
                     YKTTFVVLFHIDNKQKYTNRLTSEINFNILKQIIKKMIFAWSIFDIVNNLSRGIILFE
                     LLIIELKPFEAAIISSITASSLSYLSINLISQHVKIFSSSKKKDTKI"
     gene            6333..6539
                     /locus_tag="VF222_12655"
     CDS             6333..6539
                     /locus_tag="VF222_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143317.1"
                     /translation="MTQFLLSSLKQINCTLSGHDISVEDEKYLFKCQEGYLNTKCVRC
                     NYSLILRMDPTDTEEYTYMILEKN"
BASE COUNT         2466 a         1001 c          988 g         2762 t
ORIGIN      
        1 aaagtaatag gttgctggta gttgtctaga aaagatttcg atttctctaa tggaatcaat
       61 attcattctt ctttgatgat tctgaaaact tggtattccg gtttttttgt ctaatacaac
      121 aatctcacca tccaaaattg cggattcctt acactttata gctgactgag atgacgatgt
      181 tatctgctct gactttaatg tcattactag ctccggatat ctgtgagtga tgaatctgcc
      241 atttctagat tgtaattcta gtgttttgtt taacctgtta aagaatagta tagaacggac
      301 tccatcccat ttgacttcaa agacccagtc tttgctgtta aaggcctcat ctactaatgt
      361 ggatagcatt ggttttattg tgggtggaaa ctcttctact tcttcttgtt gttggttaga
      421 aaaagtgtta tcagttggct gattattact taatttgatt gctttagtaa aagagatttt
      481 tgtcttgtct tttttatggt aacgtatatc ttcttcttgt tctttttctg aatccttatt
      541 tattcctctg gtcaaaacag aatttggcat agtaatagtt agatcttcat tagattcgaa
      601 ttcatcagct gatttgatta atagccattg ctttccttct tttcctttat ctaaatgagt
      661 cctaactaag gtaaactttc cccttagttt atgaccaaat aaagtaaaag taatctttcc
      721 tttttgaaat tgatctgata tctcctgctc tgatgtgtat gtgcctgtat cccaaacaat
      781 tacagtacct gcaccataac ttccctgagg aattacaccc tcaaagagca aataatctaa
      841 tggatggtct tctgttaaaa ctgcaagcct ttttattttt ggattaagtg aaattccttt
      901 tggtacggcc caagatttta atactttttc ttttgtatct tctaacctaa agtcataatg
      961 taatctacta gcatcgtgct tttgtactac aaatttgcta cttttattcc ctgtgagccc
     1021 ttttttgttt ccttgaggct caggtgttct accaaagtct ctttttttat tatattgctc
     1081 taacatttta tgcccctaat ttacaatata tagaagaaga gaaacaacat taatgatata
     1141 ctcagtcaat aatatcatac caaaagtaac aacatttttt aatttctcat cggaaagtct
     1201 aaaattattt tctgtatgtt tcttcattct tcttttaccg atcggctcta ttacctcaac
     1261 ttttaggtcc cattcataat cgctcaacta acatttgtag aactattgtt aaagtttctt
     1321 taacactttc accatgtagt gttggcaaat ccattgttat taattattat tttccttcat
     1381 catacctcat caacacacca agtgtcttgt tcattggatg tttacatcta caaccatggg
     1441 tgcttagacc atgaagtgaa aacttatgac ggcatctata gtaggtacat attggttcag
     1501 actcttgacc gaatacatca attattagtt gtggttgttt ttgctgtatt tctttatctg
     1561 ttgattcatt tgttgtagat agtgttggcg atgatgatga tgatgaagat gatgaagatg
     1621 atgaagatga ggataattca ataatcctat tctcaagcag ctgcatttct acggttagcc
     1681 attccttgat agctataaat taaatcactt tttatgtaga tagattcagc tccattttca
     1741 taagatttac attcttgatt tctaaaattc atggaatttc ttaaacattt atttggagcc
     1801 gttgttccta tttcgagttc tgattctatt gcggctgcta ttgttttttc tacctgggaa
     1861 agcattcaat atctacctcc actaatttct tttttgtcta cggggcttgt ttttcgtatt
     1921 aatactagct ttaagagcct cgagtaggtt tgtagtatct tcagcttttt cttcttctgc
     1981 ctcaatagta accttctgtc ctttagattt tgcttctatt agtttctcta gttctttaga
     2041 gtaggtatcg gaatatttac ctatatcaaa ttgttcactg gttaagttat caactaatgt
     2101 ctttcctaaa gataattcct tatcttcgac cttaggctgc tgactgctag aaataccttc
     2161 aacctcatcc atgggtttta tctcatctag atattttagc tgatgcatta caaggcctcg
     2221 ttgatatgat cttaaagcaa ccaaatgttc tttatctctt aacactacct tacctattgc
     2281 tactttatta gtctcattaa gaatcttgac aaatagagaa tacgctttgc tactggaagg
     2341 ggatgtattt ttcttcttct ttgttattcc agcgtctgga ccaatataat agttcttctc
     2401 gataaaaaga gggtcaaagt cttttgaatc tatgaattca ttaatctcta ttgtgtgtgt
     2461 agtttttaat ttgatattct ctaaatcttc tttttctaaa ataacataat tattttttgt
     2521 aatttcgtat cctttcttta tttccgacca aggaacttcc ctttcttcta ctgggcacca
     2581 ttttttatat ttaattttgt gacctttttc gtctaattgg ttaaaagagg aaaattcatc
     2641 tttttgtgta gctacaaaaa cctgaacggg tatgttgact agtccaaagg atatgcttcc
     2701 tttccatata gtgcgagcag ccattactaa tgtacacgct acaacccttt aaacaactac
     2761 ggaataaaga tatgtatatc tttagctgta taatttttca gtataattat agagaaaatt
     2821 ttatttatat aatttaagca aaaaataaaa tgaagctaca aattcttttt tattattatt
     2881 accgaaataa tatttcgata tatactcgtt atactgattg ctttgtttgg aagagccatc
     2941 ttaactttca acaataaaaa tcacaatagt gacatgtata tagattattc taaatttcta
     3001 tattgtgact aacaagtttt tgttttgtat attatagtcg tcttcttcct aatccttcgt
     3061 cactgtctct acgtcctcta aataatttcc taattagtag ataaataaat tgagttaaaa
     3121 tgatcattaa aattctattt ttccatctca acagctataa tgatctttag aatacatagt
     3181 tataaatata ttcgagaatt aattatttat attctctatt gttatactat gggatctaaa
     3241 gaaatttgct gtggtagttt aaagcaaaac tatacagccc aattgaacca taataaaaat
     3301 aaaatatttt ttaatcaatt tcttttatta tcttctgcta atctagattc atagatgttg
     3361 actacttttg aagtagattt atagttaatt tataagtcaa ttaattataa ttaacattaa
     3421 aaaatgggtc gagaatatag agataagtta agtccatatg ttagaggatt tgaaagtgac
     3481 tcacctgaaa gtcttggaga aaaaataact acttttttaa ctgaaaatcc agaattagaa
     3541 cttaaagatg ttaagtatag ttcatatgga tttatacaca atcttagtag agaaactagt
     3601 tatagtgcat tggtgagatg taatagaaaa taaaaaatta taatcattta ttttttttaa
     3661 attatttaaa aattgataat acagtatggg tttaataatt gaataattag ttttaatcat
     3721 taatacatct atacgcaatt tcgttatcat tttgagaaaa tcaaattcaa gaaattaaat
     3781 tacaatataa tttttaacaa taccagaata aaatattttt ttcattctaa cataatattc
     3841 aaagtagtaa atatatagct atagtaattt ttaatatcca atgttaataa taacgtattc
     3901 taattaaaag tttgatttgt tacatttatc atgaagttga actaaataaa ataacaaata
     3961 caaccgttta aattgcatat aatctcttct caattaggag ttatatacat aaagctaaga
     4021 aactcctctt tgtattatac ctatttaaaa ggacggacat cagataattt gaatttatta
     4081 ttaattttgt ttttaaaatg cataatttgc atcaatttat tcttatttta ttagagcaat
     4141 cgttacaaac ttttaataat gtatataatg tgcattttat tcaaatagat aattactaaa
     4201 agaatatatg aaatatcata ttaatggaca cataaaatgt atttatattc tagtatttct
     4261 ataactataa gaagaaagga aatgggaata ataataagaa aaaatgatga atttactaca
     4321 ttattacaaa ataaacttaa ggaaagatat gagcaaagaa gaagattaga taataaagta
     4381 catgtatccg atattattcc tacttcctgt ataaggaaac aatattactc ccgtaaatat
     4441 cctgaattag acgttatatc gaatgaaact gtccagcatt ttgtaagagg ggaagcaagt
     4501 gaattagtta ttactagtct tgcagatttg ggagtatcac aatcagaatt agaaatggat
     4561 ggattgactg gtcgtcctga cataatgaat cgtaatatta ttgttgaatt aaaggatacc
     4621 atgaatccac aaagattgaa cgttactgat gataaattta aatcatatct cagacaatta
     4681 ctatactatc ttgtaataac aggaatagaa aaaggtatta tttctataaa atataatatt
     4741 aaagaattaa aatgggttaa aagtgactct catggagatt atttctttcg accttttaat
     4801 tcaaaatcgc ctgaaataga aagttgggaa atatttctac ccggtaatga tattaccagg
     4861 gaaattttaa aaaatgaaat gattagaaga aagaatcttt ttctaaaagc tctagaaaac
     4921 gaagatgtta ctatattacc aagactttca gaaaaaatta gagaccgaaa atgtccttgg
     4981 tgtaaatttt atgaaatttg tatggatagt aagaatgatg aaaccgaaga ggctaacgaa
     5041 atggcaaatg aaatagatat ctttgatata ccaggtgtta tagatttaaa gattaataag
     5101 accactactt caattactta attcaaaaat tatgctaaag attcagtgga tgaatatgac
     5161 caaatttgta ttgaatagaa attgactttt ttgttcctat tcaagttctg gtggaccttt
     5221 taagtataac tattggaaga ttatcattta atattttgta tgctggtttg atattatttt
     5281 catatctttg tatctttctt ttttgatgat gaaaagattt tgacatgttg tgagattaaa
     5341 tttatagata aataagatag agatgaagca gttatagaag atattatggc tgcttcaaac
     5401 ggtttgagtt ctattattaa taattcgaat agtattatcc ctcttgataa attattaaca
     5461 atatcaaata tagaccatgc aaatatcatc ttctttatta tttgttttaa aatattgaaa
     5521 ttgatctcgc ttgttagtct attagtatat ttttgtttgt tgtcaatatg gaaaagaact
     5581 acaaaagttg tcttatagat tatgaatcca actaatactg ttattgtaga agttaataca
     5641 tcactaggag aatataaggc atatagttca gctattacag cactagctaa aaaaccaaaa
     5701 atgcctgcga taagtaaatt cttattcatc aaaatggttt ctctatattt cttgtaaaaa
     5761 ctagtaagaa atctattggt aattctgtta ttactgatat catttttttt atccaataac
     5821 aattatgtat atacaaacaa ccctttatat ctttcaaaag aagaaatcgt ttttggaaaa
     5881 agtggagaaa ctcaaattta gttttactta aatttaacaa tataacattc aaaaagctat
     5941 attatttgaa ataatatttt tacatatcat tatactataa aattattaca attgtgtcca
     6001 aggtcatatt tgctgatatc gttgatgatt tttcttgact atttttactc tatttatcaa
     6061 gttcaagcct ttctatcaat tttgatttgg atggaattac gttatcatta tttgaccttt
     6121 atacgatata ttatgacgaa gataaaaata atattacccc tttttttaaa tagataagaa
     6181 tatgattaat cgagataaaa atatgaaaat aataactaat ataattagac aagatagcta
     6241 atattttttt cttctggtac ttataaatag tattttttta ctatataaaa ttataggaca
     6301 aggttaatta tagggataac gtataaaata atatgactca atttttactt tcgtcattaa
     6361 aacagattaa ttgtacactt agtggacatg atatatctgt cgaagatgag aaatatttat
     6421 tcaaatgtca agaaggatat cttaatacaa aatgtgtaag atgtaattat tctttaatat
     6481 tacgtatgga tcctactgat acagaagaat atacatatat gatattggag aaaaactaat
     6541 cagagttcat ttctctaaag tttaacgtga cggatttatt tgtttaaact attaaattaa
     6601 ttggaataat attacttgtt ttgatggaac ctctaaataa agattaaagg acatagaatt
     6661 tttgtgatgt tctcttattg tcaagctatg agttttcatt tgtattacat atttttatta
     6721 ttactatagg tacataatct ttaaattaga aagataaagg taattaaatt ttattaactc
     6781 taatactttt ccttcttttt gataatattt agataaagaa tattgattaa gatatttata
     6841 atctttaaat ccttcagatt tccaatagta cttattgact aaataataac tcacaatcat
     6901 ataagactgt ttacaattat ggaaagaaga caaacctaaa tagactgtca tattattcag
     6961 gtaatagtac ctcccagaaa ttaaaataat tgacgtttaa tattgatttt aataatctgt
     7021 ccaatatctt attactaact attagtggaa gatttgtcta ttagaattat agaattatta
     7081 aagttcatga tattcttatg aatccaatta aaatatgata tcaaaaaaat taacaaaaca
     7141 ataaattatt aaataatcgc taatttaaat actcaactct aatacatcaa caaatgaaaa
     7201 tttctttttt actattt
//
LOCUS       DASVEV010000289         5450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1712906, whole genome shotgun sequence.
ACCESSION   DASVEV010000289 DASVEV010000000
VERSION     DASVEV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712906"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF222_12660"
     CDS             complement(<1..391)
                     /locus_tag="VF222_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143318.1"
                     /translation="MLLVEGFVKEKGVKTEVCELFLTRFCSSLLEIFIKDISYYPIGK
                     TIMALYIVRARPKNDLSSLRKELNSGEISKLRPFGKTLQYGLDNAKIDSKDGYALWIE
                     EDYCTPPLAMERESVLDRYFEDITVQLI"
     gene            complement(431..1192)
                     /locus_tag="VF222_12665"
     CDS             complement(431..1192)
                     /locus_tag="VF222_12665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7143319.1"
                     /translation="MANQNSITENDPYSKEQLSMYFDPFIYKHIKELRLDGVNINKLI
                     IVFWDISKFSALIKELKALMKKRIKKQGLIFHELEYLLRDYYSEATRVIKKNNGILDK
                     FIGDGIFAYFGYQDKKFDIVHSKAMNAALELKTNFIKIKEKHMKILYSHYDYKPTTNI
                     NLKCAMHIGQVVFGYWYSPLRSQITAIGDDVNFCSRLEGFADNDQIIISKELNNALRK
                     ITKNTFRTKKIKIPEDKKLKTYEHVKYVYELIGKK"
     gene            complement(1206..1610)
                     /locus_tag="VF222_12670"
     CDS             complement(1206..1610)
                     /locus_tag="VF222_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7143320.1"
                     /translation="MKHQVIIPQFLSNESIRNYIPPTVKIQKGDIITWINTNNESHHL
                     YFIKIDPDPTNIEVLDERLYLNSGEVGEIMFNYNYERIDYVCKIHRREVNSIVIFTED
                     YKNMSNTQRLRYLRKRYKIKIPNLSNYLDGNR"
     gene            2327..3313
                     /locus_tag="VF222_12675"
     CDS             2327..3313
                     /locus_tag="VF222_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7143321.1"
                     /translation="MLKFLSFLFGIITFSIILFSANSSFISIYAQDNQYYYNTTNENN
                     NSSLFNELESIKNILESKVTKLATALQIVSYLPQILQPPDVNLIDPKVKGIPEDADIE
                     KRKIGKILLDQFEFSSIDFLLNNGNIYFDEPFEDQLNRTVTNLSFRDYYQAVNQTKKT
                     YLSDAIISKTTGRNLAVIATPVINKENNMTGILLGTINFKNYDKFLQSLNLQNNSRLV
                     LIDKNGVKIGDSNENETSVSKESFEKKQFSNLTSFKLALEGKSGSTVEKFDGKERQIT
                     FLPYDLFQNKRILLLIQGCMSNVTNSNLCIDNNNKELHLFNENVLTRLGSFF"
     gene            4262..5383
                     /locus_tag="VF222_12680"
     CDS             4262..5383
                     /locus_tag="VF222_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7143322.1"
                     /translation="MSDTSRTLRQYPLPDKRKKTIILPSSSASSISEKRDEKSESIAV
                     DKFIESQIGLVSKSNIERWINCLTSFHNRHSKSIYNHQVASWLKKELEISGYKDKDKI
                     KDEDKDKYKDVVYFHQFKENGLELKNVICHKQGETNKFVLICGHYDTILGDNFEDTVS
                     RAPGANDNASGVAAILEVARILFSVNLKYSIRFVLFSGEEQGLRGSEHYADAIKRKND
                     DLSLVINLDMIGVPDYHAIKTTIEKEDMVVQHDFSASKKTIQIDVDKEFEKQPSCNEI
                     KENDEQSDRYGTIMEQMALNYTNIKGKKGSVYSSDYCPFEARGYVVIGAYDQSAEPDN
                     PHYHSCSDVTTNLDMEFLASVTKLVLATILHVTRQASSF"
BASE COUNT         1982 a          795 c          753 g         1920 t
ORIGIN      
        1 caataagttg aaccgtaata tcctcaaaat acctatcaag aacactttct ctttccatgg
       61 ctaatggagg agtacaataa tcttcttcta tccataaagc gtagccatct ttggaatcaa
      121 tttttgcatt atctaagcca tattgtagtg tttttccaaa cggtctaagt tttgatattt
      181 caccagaatt caattctttc ctaagactag acagatcatt ttttggtctc gctctgacta
      241 tgtataatgc cattatggtt ttacctatag gataataaga aatatctttt ataaatatct
      301 ctaaaagtga actacaaaat cgtgtcaaaa acagttcaca cacctcagtt ttcacgccct
      361 tttctttaac aaatccttca actaaaagca ttgaatctta atgttcataa atattcaatt
      421 tagaatttgc ttattttttg cctattaact cataaacata tttaacatgt tcataagttt
      481 tcaatttctt atcttctggt atttttattt ttttagttct aaatgtattt ttagttatct
      541 ttcttaatgc attattcaac tcttttgaaa tgataatttg atcattatca gcgaatccct
      601 caagtctact acagaaatta acatcatccc caatagcagt aatctgactt cgaagaggag
      661 aataccaata tccaaacaca acttgaccaa tatgcatagc acatttcaaa tttatattgg
      721 tagtaggctt gtaatcataa tgagaatata aaattttcat atgtttttct ttaattttta
      781 taaaattcgt ctttaactct aatgcagcat tcattgcttt tgaatgaact atatcaaatt
      841 ttttatcttg gtaaccgaaa taagcaaaaa ttccatcacc tataaattta tccaagattc
      901 cattattttt ttttatcact cttgttgctt cactataata atctcttaat aagtattcta
      961 attcatgaaa tataagacct tgttttttta ttcgtttttt cattaatgct tttaattctt
     1021 taattaaggc agaaaattta cttatatccc aaaatacaat aattaattta ttgatgttga
     1081 ctccatctaa tcttaattct ttaatatgtt tatatatgaa tggatcaaaa tacatactta
     1141 gttgttcttt agaataagga tcattctctg taatactatt ctgattagcc atatatttac
     1201 accaattatc tatttccatc aagatagttt gacaggttgg gaatcttgat tttatatctc
     1261 ttacgaagat atcttaacct ttgagtattg ctcatatttt tatagtcttc agtaaaaata
     1321 acaatactat tgacttctct tctatgtatt ttacatacat aatctattcg ttcataatta
     1381 taattaaaca tgatttctcc cacttctcca gagtttaggt aaagcctttc atctaatact
     1441 tcaatatttg ttgggtcagg atcaatttta ataaagtaga gatggtgaga ttcattattc
     1501 gtattaatcc atgtaatgat atctcctttt tgaattttga cggttggcgg gatataattt
     1561 ctaatagatt cattacttag aaattgtgga atgattacct gatgtttcat cactattatg
     1621 taattatatc aaataggata tatagaaaca ccaacagaca acattgaaga aacaaaaaaa
     1681 atttctattc gaatatattt ggctgggaat tggagaaaag aggagaagga gaaaagtagt
     1741 ggttaataaa aaatgccgga ataaaaagag caataacttc aaaaagagaa gataatcaaa
     1801 ctcctacatt ttatattatt gtagaatcaa tagatgactt tataactaaa tctcaaaagc
     1861 aaagtgcaaa gaatataatt aacaaaaagg agacatctga aggttttatg ctatcattac
     1921 aagatcagca acaaaatact tttttggttt atggcaaagc aaaaaatagg taagtaaaag
     1981 cattgtctag attaaatcaa acccataata ttttttgaac caaaataagt tactagattg
     2041 aattataaac tgaagatatt cctggcgaaa tgtctaactt atgattcgta agaaatttta
     2101 ttagtaattt tgcagttaat gcagcaatgt tatcagactt aaaattcata aacttattac
     2161 tttttgaact acaaaatcgt gtcaaaatca gttcacacaa ctccgagtcc ttactacgat
     2221 attaatgttt tcaacaatat catcttatat aatatatcaa caaccaaatt attctgtcat
     2281 agatagaatt atattgaatt gttcttaaaa ttctcaagat taaataatgt taaaattttt
     2341 aagtttttta tttggtatta taacttttag tattatttta ttttccgcaa actcatcttt
     2401 tatctccatt tatgctcaag ataatcaata ctattataat accaccaacg aaaataacaa
     2461 ttcttcactt tttaatgaac ttgaaagtat aaaaaatata cttgagagta aggtaacaaa
     2521 actagctact gctctacaaa ttgtgagtta tttaccgcaa attttacaac ctcctgatgt
     2581 aaatttgata gatccaaaag ttaagggtat accagaagat gcagatatag agaagagaaa
     2641 aataggtaaa atactattgg atcaatttga attttcatca attgattttt tattaaataa
     2701 tggaaatata tattttgatg aaccatttga agatcaatta aatcgaactg taactaatct
     2761 ttcatttaga gattattatc aagcagtaaa tcagactaaa aaaacttact tgagtgatgc
     2821 aattatctct aaaacaacag gaagaaatct ggcagtaatt gctactccag taatcaacaa
     2881 agaaaataat atgacaggaa tactgttggg aacaattaat tttaagaatt atgataaatt
     2941 tctgcagtca cttaatttac aaaataatag cagattggta ttaattgaca aaaatggagt
     3001 aaagattggt gattctaacg aaaatgagac gtccgtctca aaagaatcat ttgagaaaaa
     3061 acagttttca aatcttacct ctttcaagtt agcattggaa ggtaaatcag gaagtactgt
     3121 agaaaaattt gacgggaaag aaagacaaat cacttttctt ccatacgatc tctttcaaaa
     3181 taaaagaatt ttacttctca ttcaaggttg tatgagcaat gtaactaact caaatctctg
     3241 catagataat aataacaaag aacttcacct ctttaatgag aatgttttaa ctagactagg
     3301 tagttttttt taaggtaact ttttattctt atatcttttt tattattcta tattaaactt
     3361 ttcaaaatgg atattagtct tagttaaaat ataatgaaga catctcaaag ttaaaaatta
     3421 tttttattat aatattttta aatattacat acttcactaa taaatcttat atctcataat
     3481 aagattaatt ttctggataa accttgattt tgaaatagat ttttgaacta taaaattgtg
     3541 tcaaaatcag ctcacactcc tccgtttgag cgcctgcgga taagtttaga acattctaga
     3601 gcatgattca atttcttgat taaaaacttt taattgttaa gtttgtgatc aataatattg
     3661 actgattaag agtaacatta gataaatgat cattattcta taaacttttt tgatgaaaat
     3721 atttacttta tcaaaaaaat aaaaaatgat aattgtttat cagtaagaga ttatttttta
     3781 cctgttaatg cacaagtatt gtttggatct atggctaaag ccttgtcata atagacgata
     3841 gctagcttgt aatttcctat tttatcaaga aaattgcctt ttcagctaat gccggaatat
     3901 atcttggatc tgtggctaaa gccttgtcgt agtagaccac tgtttcttat gattccccaa
     3961 gttatcaaga gcataccctt taccaattaa tgcaagaaca tatcagaacc gtattcaatc
     4021 aacggttgat tgtaattttg tattattata ataatttctc tattcactaa ggtatagata
     4081 cttgatttat tattattctg gctataaaga atttgtttat atatcttgat tacaccgaaa
     4141 attcaaatga aactaataga attcaacata tatcaaatta atgaactatc aaaaaagcat
     4201 cagctaaata attttgtctt gtatattaac gcggcagcat tactagagaa agtgaaaagc
     4261 tatgagtgat acgtctagaa ccttaaggca atacccccta ccagataaaa gaaaaaagac
     4321 tataattcta ccttcgtcat cagcatcttc tatttctgaa aaaagggatg agaaaagtga
     4381 atcaatagca gttgacaaat tcatagaatc acagatagga ttagtatcta aaagcaatat
     4441 cgaaagatgg atcaattgtc taacatcttt tcataaccga catagtaaat caatatataa
     4501 tcatcaagtg gccagttggc ttaaaaaaga gttagaaatt tctggttata aagataaaga
     4561 taagattaag gatgaggata aagataagta taaagatgtt gtttattttc accaattcaa
     4621 agaaaatgga ttagaattga aaaatgttat ttgtcataaa cagggtgaga caaataaatt
     4681 tgttcttatt tgtggacatt atgatactat attaggtgac aattttgagg atacggtatc
     4741 tcgagctcca ggagctaatg ataatgctag tggagtagca gcaattctag aggtagcacg
     4801 tattctcttt tcagttaatt tgaaatatag tattcgtttt gtattgttct ctggcgaaga
     4861 acagggttta agaggttcag aacattatgc tgatgctatt aaaagaaaga atgacgatct
     4921 tagtctcgtt attaacttag atatgattgg tgtaccagac tatcatgcaa ttaaaacgac
     4981 catcgaaaaa gaggatatgg ttgttcaaca tgatttttct gccagtaaaa agacaatcca
     5041 aatagatgta gataaagaat ttgaaaagca accatcttgt aatgagataa aagagaatga
     5101 cgaacaatca gatagatatg gtacaataat ggaacaaatg gctctaaatt ataccaatat
     5161 caaaggtaaa aaaggctctg tttattctag tgattattgt ccgtttgaag cccgtggtta
     5221 tgtagtcata ggtgcatacg atcaatcagc cgaacccgac aatcctcatt atcatagttg
     5281 ctctgatgta acaactaatc tggatatgga atttcttgca tcagtaacta aactggtact
     5341 tgctactatt ctacacgtta ctagacaagc ttcatcattc tagtcactca tcttttgcga
     5401 tgattaattt tttaaataat aaactttatc tattatgtta ccgaaccctg
//
LOCUS       DASVEV010000290         6698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_934921, whole genome shotgun sequence.
ACCESSION   DASVEV010000290 DASVEV010000000
VERSION     DASVEV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934921"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF222_12685"
     CDS             <1..1030
                     /locus_tag="VF222_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7143323.1"
                     /translation="NIDLSSESIPSDTLRKIIVTMNDFMDVIKEMEPSAMREVFVEIP
                     DIRWEDIGGLASIKQEMQEAVEWPLKYQGIFTFADAMPPKGILLYGPPGTGKTLMAKA
                     AANESEANFISIKGPELLSKWVGESEKGVREIFRKARQAAPCIIFFDEIDAIAPKRGG
                     NFGDSHVTERLISQLLTELDGLEILTNVVVIGATNRPDIVDAALLRPGRFDRLLYVPP
                     PDRDSRIQIIKIHIKKKPLDDVVDIEKLADQTEGYTGADIASLSSAAVMLALREHVSK
                     YKDPKEAEDHKEELKIHMKHFEDAMKKIRPLSTQELNMYKIISEQFGQPDISSKAKGP
                     ELQKGFIS"
     gene            1535..3046
                     /locus_tag="VF222_12690"
     CDS             1535..3046
                     /locus_tag="VF222_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7143324.1"
                     /translation="MKTTAKCTPKNNSNKQNLLYHRFFPTIYIRYSEYFHYMLSLKFK
                     TINPATEEIINEYENMTYDHLDNILMNAKNAFVLWKEDFKKRSNYLYAFANEFRKNKD
                     NLAKTATLEMGKPIRESKTEVDKCVWAMEYFADNGQIFMTDEIINTDARKSFITFQPL
                     GVIASIMPWNFPFWQALRFAAPSLIAGNTIVLKPASSTMQCGLEIEKIFNKVGLPNGV
                     FQTVIGNSTIAEYMIDSKNVDAVTFTGSVPVGSNVAKRSTSHLKKCVLELGGSDPFIV
                     CDDADIDKASLGAVKGRFINCGQSCIASKRFFVVKKVANEFIEKFVQKTEKLRIGDPL
                     DETTDIGPMVSLQSLEFIDKVVHDSIKEGAELLIGGQRLGKKGYFYTPTILKDVVPGM
                     RVVDEETFGPVAPITIVDDEKEAIRFANDSEFGLGASIWTQDLDKAERLSRLVQSGIV
                     TVNNVVMSDPRVPFGGIKNSGFGRELSKYGILEFVNIKSVRFYDQLIRNHHVE"
     gene            3309..4868
                     /gene="glnA"
                     /locus_tag="VF222_12695"
     CDS             3309..4868
                     /gene="glnA"
                     /locus_tag="VF222_12695"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7143325.1"
                     /translation="MNLHKNSSTHFKNEFNEYNDKEDDDNNNNHIEFIQIRYTDVIGK
                     FLAKYFQTDKNHIYEIFRTGIGLDGSSVKGFSTIDESDMLLLPDRTTERKITMSDNKI
                     TTVIADVYRGFNQGRSSRDPRYVSQTMEEYLAMNQITCQVGAEVECFIFDDIIFSNNL
                     ATTSSVEPNEANPIQKNLQSVHSNPDLTNVSVLSSEQVGKGKYPIRPKSGYDTTPFQD
                     SLVDFRFEIARILKKYYSIEVTNLNHEVASFGQIEINFMHDILTKTADNVQLYKDVVR
                     NVAKRYNKVANFMPKPIFDENDPTRGDNGSGMHVSVSFWKKSTSKTANSNIFYDSDDS
                     YSELSQTGRYFIGGLLDHASSLSSLVTPTVNSYYRIIPGFEAPVYLAWARGNRSAVVR
                     IPVNAKGNDSSKRIEFRAPDPSANSYLAFSAIVAAGLDGIKKKIDPGNPINENIYKMS
                     DHKRTSFGIKMLPSSLNEALTSLKSDSEYLNICFEKPLIEVYISLKLQEIAEIGNDKS
                     KLKQFLYYYDV"
     gene            5504..5818
                     /locus_tag="VF222_12700"
     CDS             5504..5818
                     /locus_tag="VF222_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143326.1"
                     /translation="MTFYLVKAKVKNHILELPQEMESGKIKKLVPYGNSLHYSLENAK
                     RDDKDGVSALWIEEDYCSPPLVMERECVLDRYFDDLIVEIVESEKAGWAKIKDKPSIW
                     AE"
     gene            complement(6138..6413)
                     /locus_tag="VF222_12705"
     CDS             complement(6138..6413)
                     /locus_tag="VF222_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX7143327.1"
                     /translation="MPTLIVTFIGIMATIFAVSSSIPQILKSIKTRKTDDVSIWLVIV
                     LIIGLSLWVVYGIGINDLVIMIANSIAVAINTVLLILKIKYSKDPIS"
BASE COUNT         2494 a          936 c         1153 g         2115 t
ORIGIN      
        1 caatattgat ctatcatctg aaagtatacc tagtgatact ttgcgtaaga taatagtcac
       61 tatgaatgac tttatggacg ttattaaaga aatggagcct tcagcaatga gagaagtatt
      121 tgtagagata cctgatatcc ggtgggagga tattggaggc ttagcatcca taaagcaaga
      181 gatgcaagaa gcggtagaat ggccacttaa atatcaggga atatttacat ttgcggatgc
      241 catgcctccg aaagggatct tgttatatgg tccaccaggt actggaaaaa ccttgatggc
      301 aaaagctgca gcaaatgaaa gtgaagccaa ctttattagc ataaaaggcc ctgaactttt
      361 atcaaaatgg gtaggtgagt ccgaaaaagg agttagagaa atatttagga aagctaggca
      421 agcagcaccg tgcataatat tttttgatga aatagatgct attgctccta agagaggtgg
      481 aaattttgga gattctcacg ttacagagag actaattagt caattactta ctgaacttga
      541 tgggttagaa atcttaacta atgtagtggt tattggtgca actaacagac ctgatattgt
      601 agatgctgca ctattgagac ctggaagatt tgataggctg ctttatgttc cacctccaga
      661 tcgggattca agaatacaaa ttataaaaat tcatataaaa aagaaacctt tggatgacgt
      721 tgtagatata gaaaaactag cagatcaaac agaaggatat acaggcgcgg atatcgcatc
      781 actatcatca gcagcggtta tgctagcttt acgtgagcat gtatcaaagt acaaagatcc
      841 aaaggaagca gaagatcaca aagaggaatt aaaaatacat atgaaacact ttgaggatgc
      901 gatgaagaag attagacctc tctctactca agaattgaat atgtataaga taatatcaga
      961 acaatttgga caaccagata tttcatcaaa agcaaaagga ccagaattac aaaaaggatt
     1021 tatatcttaa ataatttttt tattattaaa gtaataatta tataattatt ctattttgtt
     1081 tttgttgtat tttctgaatt gacatagagt tattttaaaa attaaaacat catcttccct
     1141 tagttttata agcctttact atgtggtgag ctcatattaa atgtaaaaga gcttacaggg
     1201 gtagaggagt tataccacca agactaaaat taatatttct catattaaga cactattaca
     1261 tttatgagtt ttaatagttt tgaaaatagt tgatgaattt aagccaaatt ttgaatttag
     1321 caattggatt agatgatatt tttcaacatc ataattccaa ttcatgtatt aaattattgc
     1381 tatcgcctgc agctatatca attatataaa ggttgttttt aatattaata taattattct
     1441 tcagttaata actcaataaa ataattagag aaaaaagtaa agtacattgg caatctatac
     1501 catacaaatt agaaaactga ttgggtcatt ttagttgaaa actactgcta aatgtacacc
     1561 aaaaaataat agcaataaac agaacctatt atatcatcga ttctttccta cgatttatat
     1621 aagatattct gaatatttcc attatatgct atcattgaag tttaagacaa tcaatcctgc
     1681 tactgaagag attattaatg aatatgaaaa tatgacatat gaccatttag ataatatttt
     1741 aatgaatgcg aaaaatgcat ttgttctgtg gaaagaagat tttaagaaaa gatctaatta
     1801 tttatacgct tttgctaatg aatttaggaa gaataaggac aacctggcta agactgcaac
     1861 tttagagatg ggaaaaccga taagggaatc aaaaactgaa gtagataaat gcgtatgggc
     1921 tatggagtat tttgctgata atggacagat attcatgacc gatgaaatta tcaatactga
     1981 cgcaagaaag agtttcataa catttcaacc acttggtgtt attgcaagta taatgccatg
     2041 gaactttcca ttttggcagg cattacgttt tgcagctccc tcattgatag caggaaatac
     2101 tattgtacta aaaccagcca gctcgactat gcaatgcgga ttagaaattg aaaagatatt
     2161 taacaaagta ggattaccaa atggagtttt ccaaactgta attggtaact caactattgc
     2221 tgaatatatg atagattcaa agaacgtaga tgcagtaaca tttacgggaa gtgtacccgt
     2281 aggttccaat gtcgcaaaga ggtctacatc acatctaaaa aaatgtgtac tagagttagg
     2341 cggaagcgat ccttttatag tgtgtgatga cgcagatatt gacaaggctt cattgggagc
     2401 ggtaaagggt cgatttatta attgtggtca aagctgtata gcaagtaaaa gattctttgt
     2461 tgtgaaaaaa gtagccaatg aatttataga gaaatttgtt caaaaaactg aaaaactacg
     2521 tataggagac cctctcgatg aaaccaccga tataggacca atggtaagcc tccaaagtct
     2581 tgaatttata gataaagtag ttcacgattc cattaaagaa ggtgcagaac tcttaatagg
     2641 aggacaaaga ttgggtaaaa aaggttattt ctatacccct acaatactaa aggatgttgt
     2701 tcctggaatg agggtagttg acgaggagac ttttggacca gtagcgccca taacaattgt
     2761 tgatgacgaa aaagaggcaa ttagatttgc aaatgattct gaatttggtc tcggtgctag
     2821 tatatggaca caagatcttg ataaggctga aagactatct cggctagtac agtctggaat
     2881 tgtgacagtt aataatgttg ttatgtcaga tccaagagtt ccttttggtg gtatcaaaaa
     2941 tagtggattt ggtagggaat tgtcaaaata cggaatactc gagtttgtta atatcaagtc
     3001 ggtaagattt tatgaccaat taataagaaa ccatcatgta gaataacatt agcaataacg
     3061 tagattctaa tgacattaac taataaaata cttaactttt atagtgtctc caagtataaa
     3121 atatatttta aagagaaaaa aatagtaaat tatcctctcc tttctcaaaa atgaaacaat
     3181 cactgttatt aacttttaat ttttaattgt aaaatatggt tttattaaaa tctttatttt
     3241 tttgttataa ttttaataaa tataattaca agttataaat ttttattcta tgattcatat
     3301 aaataactat gaatttacat aaaaattcca gtacacattt taaaaatgaa tttaatgaat
     3361 ataatgacaa ggaagatgac gataataata ataatcatat agaatttatc caaatacgat
     3421 atacagatgt cattggtaaa tttttagcta aatattttca aactgataag aatcatattt
     3481 atgaaatttt tagaacaggt ataggtctgg atgggtcttc ggtaaaaggt ttttcgacaa
     3541 tagatgaatc tgatatgtta ttacttccag atcggaccac tgaaagaaag attaccatgt
     3601 cagataataa aataacaacg gttatagctg acgtttatag agggtttaac caaggaagat
     3661 catcgagaga tcccagatat gtttctcaaa cgatggaaga atatttagct atgaatcaaa
     3721 ttacatgtca agtcggagca gaagtagaat gttttatatt tgatgatata attttttcta
     3781 acaatctagc aacaacatca tctgttgaac ctaatgaagc aaatccaata cagaaaaatc
     3841 tgcaaagtgt acactcaaat cctgatttaa cgaatgtttc tgtcttatca tcggaacaag
     3901 tcggaaaagg aaaatatcct atacgtccaa aatcaggtta tgataccact ccttttcagg
     3961 attcattagt agatttcagg tttgaaatcg caagaatatt aaaaaaatat tactctatag
     4021 aggtaactaa tttaaatcat gaagtagcga gttttggtca aattgaaata aattttatgc
     4081 atgatattct tacaaaaact gctgataatg ttcagttgta caaagatgtt gtgagaaatg
     4141 ttgcaaaaag atataacaag gttgcaaatt ttatgcccaa acctatattc gatgagaatg
     4201 atccgactag aggagataac ggatcaggca tgcatgtaag tgtgagtttt tggaaaaaat
     4261 ctacttcaaa aacagccaat tcaaatatct tctatgattc agatgattct tattcagaat
     4321 tgagtcagac aggcaggtat ttcataggag gcttgcttga ccatgcctcc tctctttctt
     4381 ccctagttac gccaactgtt aattcatact atcggattat cccaggattc gaagctccag
     4441 tttatttagc atgggctcga gggaatcgat ctgcagttgt aagaatacca gtaaatgcta
     4501 aaggtaatga ttcctcaaaa agaattgaat ttagggctcc agatccatcg gcgaattcat
     4561 atcttgcatt ttctgcaata gtggcggctg gattagatgg aattaagaaa aaaatcgatc
     4621 ctggcaatcc gattaatgaa aatatttaca agatgtcaga tcataaacgt acaagttttg
     4681 gaataaagat gcttccatcc agtctgaatg aagcattaac atccctgaaa agcgattctg
     4741 aatatttgaa tatatgtttt gaaaaaccgt taatagaagt atatatcagt ctaaaattac
     4801 aagaaatagc tgaaatagga aatgataaat caaaactaaa acaattcctc tattattatg
     4861 atgtttaaga attacagttg ttagcagaca attattgaaa tgtaaagtta tgatattaga
     4921 ggataataga ttttctgaaa tttaatttaa aataatttgc acaattttaa taattatttg
     4981 ataatttaat tgatgtttga gcaaggtcaa ggttgaagca taccacctat tctagggtat
     5041 aataaaatat cattaattct atttgatctt ttctaaggaa tccttataat aaatgaaatc
     5101 aattacttag tactatgtgc ttcatgaatg aaataccgga ggaaaagcaa gtactgaagc
     5161 ctaaaaaact cgaaaaagaa aaacaacaag aaaataaatc tcagtacgaa gaaaaaccaa
     5221 taatggtaaa cgctacctaa aaaagtcttc actatttttt attttttcac aattacaccg
     5281 tatattaata aactttaatg gagtgaaaaa ataaattatc ggtactgcat taatcatctt
     5341 gtgattattt agcactagta tatcaatata aaagccctga cttaattctt agtctgattt
     5401 atcatttaaa taatctctgc aaaaaaatat tctataatta tcgctactcc taaatgaaag
     5461 taatgggaga aaaataattt aattgatggt gatataaagg actatgacat tctatcttgt
     5521 gaaagctaaa gttaaaaatc acattctcga attgcctcag gaaatggagt ctggtaaaat
     5581 aaaaaaactt gtaccgtatg gtaattcttt acattatagc ttagagaatg caaaaagaga
     5641 tgataaagac ggcgtgtctg cgttatggat agaagaagac tactgttctc cgccattagt
     5701 catggaaaga gaatgtgtac tagatagata cttcgatgac ctcatagtag aaatagttga
     5761 atcggaaaaa gcaggttggg ctaaaattaa ggataaacct agtatttggg cagaataaag
     5821 aaggaaaaaa aaacttaaca aaccttctat tattctataa ttttaaaaaa caacctatca
     5881 ttgctattgg atcatctttg tacggctttg tttttataag ataaattagc aaaatggttt
     5941 atagtatttc taagtggtat attactaaaa aaaagccttt attaatttca tcaagaattt
     6001 caagtctcgt ctaagggata aaggtttgta tactcttcga gcactctttt gcacaagatg
     6061 ttgactgtat atatctatgc gcaggtttaa caaaacttgt ttatcaccgc ttgttataaa
     6121 ctatcagtaa aaaatgatca gctaattgga tcctttgaat atttaatttt caaaattaat
     6181 aacactgtat taatggctac tgcaatagaa tttgctatca ttatcactaa atcatttatc
     6241 cctattccat aaacgaccca taaactgagt ccgattatta atactatcac taaccaaatt
     6301 gatacatcat cagtttttct tgtttttatt gatttcagaa tctgcggaat actactggat
     6361 acagcaaaaa tagtagccat tatacctata aaagtaacaa taagagttgg caaggttcaa
     6421 ctataattat aacatttatt atatcttatt ctcaataaat caagctcgtc attttctttg
     6481 acttgataaa aaaagttatt actcaagatt aatattattt agaaaaataa aaccaaaata
     6541 tattgtagat ccaattaaca ataagtggaa ttctggtaag ggtggattga ttcaataatt
     6601 cgggcttgag aattgaccaa atgaataagg tattagaata tatgatttaa taagtaaagc
     6661 ttgagcttga ttttgtcaat taataatgct taactgat
//
LOCUS       DASVEV010000291         9287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1325634, whole genome shotgun sequence.
ACCESSION   DASVEV010000291 DASVEV010000000
VERSION     DASVEV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325634"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            714..935
                     /locus_tag="VF222_12710"
     CDS             714..935
                     /locus_tag="VF222_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143328.1"
                     /translation="MYEFKYIAVFLLSTIVIGIISLYDGLSINEMQIYGEKEDKKKYN
                     EGGFKVYNDYLIQEPSEYGKKDSSGKINP"
     gene            complement(1649..2203)
                     /locus_tag="VF222_12715"
     CDS             complement(1649..2203)
                     /locus_tag="VF222_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7143329.1"
                     /translation="MLPSLEYIRQTRQKLGITQRRLASLTGISTSMINQIETGRCKPS
                     YDTAKKIFEVLSTLEGKSAKKAGDICSKKIIFAQKDDTLYSVLEKMRLNFVSQVPVFE
                     GQKIVGIVSEDSLAKKMVENDEKNIKNMTIDTVMEPSPPLVDSLTPAKALVPLIRFAK
                     CILVTEKGNVTGIITTTDILKMVE"
     gene            2389..3669
                     /gene="prf1"
                     /locus_tag="VF222_12720"
     CDS             2389..3669
                     /gene="prf1"
                     /locus_tag="VF222_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HEX7143330.1"
                     /translation="MSFSNDKESKWDSVKRYKLNKMINKLGNITGHGTELVTVYIPPR
                     RPIYDIVSQLKNEAGTASNIKSDLTRTHVQDALSRTVEHLKLYKEPPDNGLVIFCGAI
                     PTGKGIGTEKIEIYSVVPPKPVQINLYRCDDHFWIDHLKDMMKDDKVIGILSLDTQEA
                     GLGILTGDRWEVVESLTSGVAGKHRQGGQSARRFERLRDNELNEYYRRVADYAHKIFI
                     DQYDIKGIIIGGPGPTKEGFIREEYLDYRLQNNVIAILDSSYSGSEGVRELIDKVNDQ
                     GIMADYRLMEEKKIVKRFISEVYSGKGLGIYGIYDVITSLKNGYADLVIVNDDITMIR
                     LEIKCNKCLNTQEKFIERNELMSLKQELTANACPNCGSQDLESSEQDIIEYLEELSLM
                     IGSRLEVISGKTEEGAQLASLGKIGAILRFKQNN"
     gene            complement(3730..4611)
                     /locus_tag="VF222_12725"
     CDS             complement(3730..4611)
                     /locus_tag="VF222_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7143331.1"
                     /translation="MELEYAFPLYRPPSEANSLIFQITLGCSFNQCSYCDMYRTKKYS
                     ERTWEEIKMEIDLTSNSYADTKRIFLADGDAINLSTERLIQILEYIYKKFPSLERVSC
                     YAMPKNLLQKTDVELMTLRSAGLKMLYLGIESGSDTILKKVTKGATYKTIVQSCKKAK
                     ENNYVLSCMIILGLGGKKYTLEHIEETAKIVSEVSPDYLGALNLHLDAGLHNEFMKKF
                     NEPFEFLNDMQILTELELLISQINSSSPIIFRANHASNVYSLKGTLPYDKERIIELIS
                     QLKEHPDMLKPKLLRRF"
     gene            complement(4955..5953)
                     /locus_tag="VF222_12730"
     CDS             complement(4955..5953)
                     /locus_tag="VF222_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143332.1"
                     /translation="MFIDQQALAKDEDDNDSKNNDDEDDNDSKNNDDEDEKVDTIDTD
                     KNIPITSSYSKNDKFGIEKIYPTKTGGEEWFMNMNNINDDKQFFPFGESDSKYSNSNL
                     KITKNEDLSWKIKSDNKLAKVRMNVYTSEGYHPEEIKTLDHSELEDTGYLQSEKDWKN
                     IEITGYVKLNHNNIPLNEGRFTWYNRGGHHTEGQPCEGVAYKGDIFFSGDNRFAKEQW
                     HVSYFFTDIKKNPNPIKDKWIGYKFIVYNLEKQSDTSKTVVKMESWIDYNNDGKWIKI
                     NEYTDKGKWGDSGKKCDGKKDQIISWGGPIATFRWDQADDVDFKNFSVREIQSPIN"
     gene            complement(6331..6666)
                     /locus_tag="VF222_12735"
     CDS             complement(6331..6666)
                     /locus_tag="VF222_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HEX7143333.1"
                     /translation="MLRRRDKRRYILLYASDEIITTSKDTNKKDTNNFIRILRKRFYE
                     LFGSIELEKASIHIISINNFHLPNYIILKCNLESIESVLFAISLASPPSTTIKISGTI
                     KKLISSITI"
     gene            complement(6656..7147)
                     /locus_tag="VF222_12740"
     CDS             complement(6656..7147)
                     /locus_tag="VF222_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143334.1"
                     /translation="MERNGQTPIINDTIVLRLKSRPIKLPLFKSLIVLSESETRINSK
                     LPYFDIFYMNQLNNYKIIIKQLKKLNRKNIGIEIKLRDIRSCNSDDVGKWVKSIKEIN
                     KFCKLNDNQLIISSGAVMNYETISGNTFESLLSIFDIEPIKYWRELEKWIDIKSGVYS
                     VAS"
     gene            complement(7240..7731)
                     /locus_tag="VF222_12745"
     CDS             complement(7240..7731)
                     /locus_tag="VF222_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX7143335.1"
                     /translation="MKHNNQSGILQFLSSYIDIVVHATEDKSRITNSVISTLEIPPKE
                     KFDEIEYEGHWGNKILRLTSEIYHKDAQALMEKILRSLSFIDKDNLLTNLERHIDEKG
                     NFHLRLDKQRMCKNKISISETEGIKIKFKINKNRIILNKKSGKVDDEIYFLYRRLVIS
                     SEK"
     gene            complement(7861..8526)
                     /locus_tag="VF222_12750"
     CDS             complement(7861..8526)
                     /locus_tag="VF222_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HEX7143336.1"
                     /translation="MSGRILMAKGSKRGRVRDKWKDKKWIIVNAPMAFGGNPINYLPI
                     TDIENSKGRVIENTLYDIYKEDPNQHQIKVFLQIDKLTTASASTIFVGHEYAKEYLRS
                     LVRRGSSMINFIKNYTTIDGYTFRISIVTFTQRRVNTSAKHEIRLLIDKMLNEKIIQL
                     GLDQFIQEVTLGGLSKELLVESKKIAFIRHLGIKKSKLISTPESRSIFEAKQIEAPPP
                     LTS"
     gene            8826..>9287
                     /locus_tag="VF222_12755"
     CDS             8826..>9287
                     /locus_tag="VF222_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143337.1"
                     /translation="MHKSEIKAVFLAVLLIAGTLTLGSQFNDAQASGDKKDQRDKKDS
                     RDHDQRDKKDSRDYSYDKKDKKKYEENQYKDRKSLDMYQKAPYMKDPYAKDPYDKPKY
                     PQEKPQQYQSDPYDKPKYPQEKPQQYQSDPYDKPKYPQEKPQQYQSDPYDKP"
BASE COUNT         3184 a         1392 c         1341 g         3370 t
ORIGIN      
        1 ttgctgttcc ttctatagta acatctacct taggcttaac tgtaggagaa ctgtcaggaa
       61 tgtcttgtaa agcatcaggt ccattatctt cttgaagata ggaaaatgga acggagaata
      121 ttagagaaat caagacaaga atataggtaa tcagatttat gttaatcatg atctttgatc
      181 taatatggta ttatattctc acattaataa ttttttaaaa agatttccta aaaaaaagtt
      241 atttgaaagt attggaagtt agttctccta aacgagtttt tcgttcgctt tctatagact
      301 tggaatttaa taagctataa ttgtcttttt ttaaagaaaa cttacaataa agtagtaatt
      361 gccgaaaaat ttctacattt tctatgttgt tataaataga taaactataa ccaatttcac
      421 aattatattt ctaatatata tgttcattac atttaactac catattgttc taacttcttt
      481 agctttattt ataatttttg ttaaaactct agttcataca aataccaaat taattgtaat
      541 gttaagagat aatcttatca ttctttatta aaaaggtttt cattttgaga tgaaaggcaa
      601 tatctatgtt ctttttgatt gtacttaact aatgtatagt taaaaatata caagtattat
      661 ttagcattaa atggaatcat agtctaaaaa tattgtaaag caaaaattat gtcgtgtatg
      721 agttcaaata tatcgcagtg tttctcttat cgacaattgt aataggtata atatcactat
      781 acgatggatt atccataaat gaaatgcaaa tatacgggga aaaggaagat aaaaagaaat
      841 acaatgaagg tggttttaaa gtatataatg attatcttat tcaagaacca tcagaatatg
      901 gaaaaaaaga tagctccggc aaaatcaatc cataggaata tcatgtggat ttaaagatat
      961 atgttcataa attcattaat tcgatgacta aagttcgctt tatttatcag cgatgaaagg
     1021 gaactgaata attataattt ataatgcata ttgttttatt tattgtaaat tactttcata
     1081 aacatcaatt cgggcccaac ttcctatagt tcgttgtcgt attacaagag gctttgaaaa
     1141 accaactgta aattacatac aatgaaataa tatatgtgaa caatctcccc cataggcttt
     1201 gttaaaaaac agtatcctta tcattagcaa tatttccaca ataatagaac aaagatattc
     1261 aataaacgat gtctgcgaaa taaatttctt tgactttaac taaattgcac atgcgaaaaa
     1321 ttcgcttcct agcaactaga atgtataatt tctttttaca atactataat ttatctgatt
     1381 gataaaaaca ttattgttct acctatatgt gcaatagcat ttcatatcat gcagttacaa
     1441 ggtactattg ttaaattaaa ttatatgtga agtagaattt aaatatcttg gatccttttt
     1501 tatatttata atggataaga aggaatgtat attatgtgga agcgtggttc aagagtctac
     1561 acacttaaaa ttatgtagga cttgcataga gtgtgcagat tgttgtattg gattggaaaa
     1621 gctatgatta ttgaatttaa tgaaaaactt attctaccat tttcaatata tctgttgtag
     1681 ttattatccc tgtaacgtta cctttttcag taactaagat acattttgca aatctgatta
     1741 aaggcaccag cgcctttgct ggtgtcagcg agtctactaa aggaggagaa ggctccataa
     1801 cagtatcaat tgtcatattt ttgatatttt tttcatcgtt ttctaccatt ttctttgcta
     1861 aactatcttc agaaacaatt cccacaattt tttgaccctc gaaaacaggt acttggctta
     1921 caaaattcaa cctcattttc tccaatacac tataaagtgt atcatctttt tgagcaaata
     1981 ttattttttt actacaaatg tctcctgctt tttttgctga tttaccttcc aatgtgctta
     2041 aaacctcaaa aatttttttt gcagtatcat aactaggttt gcatctacct gtctcaattt
     2101 gattgatcat tgatgtgcta attcctgtta atgatgcaag tctcctttga gtaattccta
     2161 acttttgcct tgtttgtctt atgtattcta aactcggaag cattactaaa ggttatacta
     2221 catgagattt ataatctata aattctaata caataaatat tgaattaata accagtcata
     2281 taacaataaa taatttatat ttatatgatt taatattctt tcttaaatgg tcggtgagga
     2341 tttataataa caataaaata gtctggagga gaaaaaagat ttctattcat gtcattttca
     2401 aacgataaag aaagtaaatg ggattctgtt aaacgttata aattaaataa aatgattaat
     2461 aaattaggca atataacagg tcacggaact gaactcgtta ctgtgtatat tccccctcga
     2521 cgaccaattt atgatattgt atctcaatta aagaatgaag caggaactgc ttctaacatc
     2581 aaatcagatt taacgagaac tcatgtccag gatgcattaa gtagaactgt cgaacatctc
     2641 aaattgtata aagaacctcc agataatggc cttgttatct tttgtggtgc tattcctacc
     2701 ggaaaaggaa taggaacgga aaagattgag atttattcgg ttgttcctcc aaaaccagtt
     2761 caaattaatc tttatcgatg tgatgaccat ttttggattg atcatttgaa agacatgatg
     2821 aaggatgaca aagtaatagg tatattgtca ttagatactc aagaagcggg tttgggcatc
     2881 ctcactggag acagatggga agtagttgaa tcattaactt ctggggttgc cggaaaacat
     2941 agacaaggag gccagtcagc cagaagattt gagaggctta gagataacga attgaatgaa
     3001 tattatcgta gagttgcaga ctatgcacat aaaatcttta ttgatcaata tgatatcaaa
     3061 gggataatta ttggagggcc aggacccaca aaagagggtt ttattagaga ggaatacctt
     3121 gattatagat tgcaaaataa tgttattgct attttagatt cctcatattc tggaagtgaa
     3181 ggagtaagag agctcataga caaggttaat gatcaaggaa taatggcaga ttatagattg
     3241 atggaagaaa aaaaaattgt taaacgtttc ataagtgagg tatattcagg caaaggccta
     3301 gggatatacg gaatttatga tgttataaca tcattaaaaa atggctatgc agatttggtt
     3361 attgtaaatg atgatatcac catgattaga ttagaaataa aatgtaataa gtgtctaaat
     3421 acacaagaaa aatttattga aagaaatgaa ttgatgtcct taaaacaaga attgacggca
     3481 aacgcgtgtc caaattgcgg aagtcaagat ttagaatcat cagaacaaga tattattgaa
     3541 tatttggaag aattatcttt aatgatcggt tctagattag aagtgatatc cggaaaaaca
     3601 gaagaaggtg cgcaattagc aagtttagga aaaatcggtg caatcctacg attcaagcag
     3661 aataactaat tagattatta aaaatattta aaagatatcc atattcgttt atactttgat
     3721 tccaacccat tagaatcttc ttaataattt tggttttaac atgtctggat gttctttcaa
     3781 ttggctaatt aattctataa ttctctcttt atcataaggg agagtacctt taagtgaata
     3841 aacatttgaa gcatggttag ctctgaatat tataggagaa gatgaattaa tttgtgatat
     3901 cagtaattct agttcggtta aaatctgcat atcatttaaa aattcaaaag gctcattgaa
     3961 cttcttcata aattcattat gaaggccagc gtctaagtga aggtttaagg ctccaagata
     4021 atctggagaa acttcgctca ctatctttgc tgtttcttcg atatgctcca aagtatattt
     4081 tttaccccct aatcctaaaa tgatcataca tgaaagaaca taattatttt ctttagcttt
     4141 tttacaactt tgtacaatag ttttatatgt agcccctttt gtcacttttt ttaaaatggt
     4201 atcacttcca ctttcgatcc ctaaatacaa cattttcagt ccagctgatc tcaaagtcat
     4261 taattcaaca tcagttttct gaagtaaatt tttaggcata gcataacaag atactctttc
     4321 taatgaagga aattttttat agatatattc taatatttgg atcaaccttt ctgttgaaag
     4381 atttattgca tcaccatcag ccagaaaaat ccttttagta tcagcataag agttggaagt
     4441 aagatctatt tccattttaa tctcttccca agtcctttct gagtattttt tagttctata
     4501 catgtcacag tatgagcatt gattgaagga acatcccaat gttatctgaa aaatcaaaga
     4561 atttgcttct gatggtggtc gatacagagg aaaggcatat tctaattcca tttatatatt
     4621 atacttatcc taatagtata aaatattttg ttagaatcaa aaaaataggt gtatgtgggt
     4681 cagttcgtcg cgagttcgtc attgttttaa actcagactg gagcagcata atacttcatt
     4741 cccttataag atataaactc cgttcgttaa aaatatatgt atttcatgac ttatgaagag
     4801 aaattaggtt gaataagaat aatatggagg ctgtcctggg gtacagcatc tttaattacc
     4861 actagatact ccttaaccta atcacctttt aaaattttat gtaattcctt agttttaaag
     4921 aattataatc ctaaatttta cacaaatgaa acttttaatt tataggagat tggatttctc
     4981 ttacactaaa atttttgaaa tcaacatcgt ctgcttgatc ccatcgaaat gtagcaatag
     5041 gccctcccca actgatgatt tgatcttttt taccatcgca tttttttcct gagtcccccc
     5101 attttccttt atctgtgtat tcattgatct ttatccattt tccatcatta ttataatcta
     5161 tccaactttc catttttacc acagtctttg atgtgtctga ttgcttctca agattataaa
     5221 ctataaactt ataaccaatc catttgtctt taataggatt tggatttttt ttaatgtcag
     5281 taaaaaaata actcacatgc cattgttcct ttgcaaatct attatcacct gaaaaaaaga
     5341 tatctccctt atatgcaaca ccttcgcatg gttggccttc agtgtgatga ccccctctgt
     5401 tataccaggt aaatctgccc tcatttaagg ggatgttgtt atgatttaat ttaacgtaac
     5461 cagttatctc aatatttttc caatcttttt cggattgcaa gtaaccagta tcttcgagtt
     5521 cagagtgatc gagtgtttta atttcttcag gatggtatcc ctcagaggta taaacgttca
     5581 ttcttacttt tgctaactta ttatctgatt taattttcca cgataaatcc tcattttttg
     5641 taatttttaa attggagttg gagtattttg aatctgactc accaaaagga aagaattgct
     5701 tatcatcgtt aatgttattc atattcataa accattcttc tcctccagtt tttgtaggat
     5761 atattttctc tattccaaac ttatcatttt ttgaatagga gctagtaatt gggatatttt
     5821 tatcagtatc aatagtgtct actttctcat cctcatcatc gttattcttt gagtcattat
     5881 catcctcatc atcgttattc tttgagtcat tatcatcctc atcctttgcc aaagcttgtt
     5941 gatcaataaa catattattt atctgaatat taaaacaaag taaaattaat aacgaaagaa
     6001 gtgaaatttc acagattaag tatttcaatt gaattacttg atataaaaat tttttttatt
     6061 ttaatttttt ttatgaatgt tctaatataa tattatgata atttgttatt aaagaggaaa
     6121 atattagaat agaaaattga attgtggagg agtcaattga taaatgtgat aaaattctat
     6181 ctccatcatg atatattata atagtcattc tctcgttggg ttaatagatg ttggtttttt
     6241 ggaaggagat ttttttctca aggtattttg taaacagttg taacgcactc atataaaatt
     6301 tatgttacta caatatagta tacatgtttt ttaaattgta attgacgata ttaatttttt
     6361 tattgtacct gaaattttga tagtagttga tggaggagaa gctagagata ttgcaaataa
     6421 aacgctctcg atactctcta aattgcattt tagaataata taatttggaa gatgaaaatt
     6481 atttattgaa ataatgtgga tacttgcttt ttctaattca atggatccaa agagctcata
     6541 aaaacgcttt cttaaaattc taatgaaatt atttgtatct tttttattcg tgtcttttga
     6601 agttgttatt atttcatcac ttgcatataa taatatatag cggcgtttat ctcttctacg
     6661 aagcaacaga gtagacacca cttttaatat cgatccattt ctctaactcc ctccaatatt
     6721 ttataggttc aatatcaaat atactaagta acgattcaaa ggtatttcca gaaatagttt
     6781 cataattcat tactgctccg cttgaaatta ttaattgatt gtcatttaat ttacaaaact
     6841 tattaatctc tttaatagat tttacccact ttccaacatc atcagagtta caacttctta
     6901 tatcccttaa ttttatttca attcctatat tttttcggtt tagttttttt aattgtttta
     6961 ttattatttt ataattattt aactgattca tgtaaaatat atcaaaataa gggagttttg
     7021 aattaattct agtttctgat tccgaaagaa cgataagaga tttaaacaaa ggcaatttta
     7081 ttggcctgct tttaagtcgt aaaactatgg tatcattaat aataggtgtt tgtccattcc
     7141 tttccaaata ctcattagaa ttatctgttc ctatgatatc taaatgatga atttttctcc
     7201 attttaaact ctctttctgt ggaagattaa agcataaatc tatttttctg aagatataac
     7261 taacctccta tataaaaaat aaatttcatc gtctactttt ccactttttt tgtttagtat
     7321 aattcgattt ttattaattt tgaatttaat tttaattcct tcagtttctg atatagaaat
     7381 tttattctta cacatccttt gtttatccag tctcaaatga aaattcccct tttcgtcaat
     7441 atgtctttct agattagtaa gtaaattatc tttatcaata aaagacaatg atctcaaaat
     7501 tttttccatt agggcttgag catctttatg gtaaatttca gaagtcaatc ttaatatttt
     7561 atttccccaa tgaccttcgt attctatctc gtcaaatttt tccttaggag gaatttcaag
     7621 tgtcgatata acggagttag ttattctact tttatcctcc gtggcatgaa ctactatatc
     7681 aatataagaa cttaagaatt gtagaattcc actttgatta ttatgtttca atttgattag
     7741 ctatgctatg ttttgttgta gttatttatc tatttaatac ttaagctaag actaaatgac
     7801 caatgaggat gtgaaaataa atttgttcat ttaaacaagg aaatactgat ttgacaaatt
     7861 ttatgaagtt agtggaggag gagcctctat ttgtttcgct tcaaatattg agcgggactc
     7921 aggagttgat atcaatttag attttttaat tcccaaatgc ctaataaagg caattttctt
     7981 ggattcaacc agtaattctt tacttaatcc tcctaacgta acttcctgaa taaattggtc
     8041 gaggcctaat tggattattt tttcatttaa cattttgtct attaataatc gaatttcatg
     8101 ttttgcagat gtatttactc tcctttgcgt aaaggtgact attgaaatac gaaaagtata
     8161 accatctatt gtagtataat tcttaataaa atttatcatg gaactacctc gccttacaag
     8221 actgcgtaaa tattctttag catattcatg acctacgaag atagtgctag cagaagcagt
     8281 agtaagttta tctatttgaa gaaatacctt aatttgatgt tgattcggat cctctttata
     8341 aatatcatat aaagtatttt caataactct tcctttagaa ttttctatat cagttattgg
     8401 aagataatta atagggttac caccaaaagc cattggcgca ttgactataa tccatttttt
     8461 atctttccat ttatcacgaa cacgacctct ttttgatcct tttgccatta gaattcgacc
     8521 tgacaaagta tattaattag ttatataagt ttatggaaca aaaattgcta tttcttattc
     8581 aaaaaatata tatctttcca cctttaactt agttaaaccg attagaagag aagaattatt
     8641 gtggtagtgt tacaactaat aataatagta aaacttaaca ataatagtag aaaatatttt
     8701 ataaaataaa taatatcttt tattagaatt tcgtatacaa tcaaaaatct attagaaaaa
     8761 caaatttcat gaatctgtta taatgcttca caccgtagat atattatagc tttgaaatct
     8821 ggttaatgca caagtctgaa ataaaagcag tttttctagc tgttttatta attgctggaa
     8881 ccctcaccct cgggtcacaa ttcaatgatg cacaggcatc gggcgataag aaggatcaaa
     8941 gagataagaa agattctcga gatcatgatc aaagagataa gaaagattct cgagattact
     9001 catatgataa aaaagataaa aagaaatacg aagaaaatca atataaggac agaaaatcac
     9061 ttgatatgta tcagaaagct ccctacatga aagatccata tgctaaagat ccatatgaca
     9121 aaccaaaata tccacaagag aaaccacaac aataccaatc tgatccatat gacaaaccaa
     9181 aatatccaca agagaaacca caacaatacc aatctgatcc atatgacaaa ccaaaatatc
     9241 cacaagagaa accacaacaa taccaatctg atccatatga caaacca
//
LOCUS       DASVEV010000292         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1131006, whole genome shotgun sequence.
ACCESSION   DASVEV010000292 DASVEV010000000
VERSION     DASVEV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131006"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1352)
                     /locus_tag="VF222_12760"
     CDS             complement(312..1352)
                     /locus_tag="VF222_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7143338.1"
                     /translation="MLFSTTSILIANISLFTSIYAQENQTETTIQVPEAAKGPAIPSK
                     GYLVEEIHDGLFWVTEGAYNTIFLVTDQGVVAVDAPPTIGKNYLKAISEVTDKPITHV
                     IYSHAHIDHIGAAGMFPKNAVIIAQEETAAELQRAKTVATNASMVPPIPTKTFSNNYT
                     LQVGNQTLQLDYYGDNHDPGNIFIYAPKQKVLMLVDIIFPGWVPFPYLAVSKDIAGYI
                     KAHDIVLNNYDFDTFVGGHLTRLGTINDVIVQKEFISDLEKTAGKSNNEILFSDIAKQ
                     VGIFDNPWLIFSKYIDAVNEKCVQDMLPKWENKLGGAQDFMSTHCFAMSQAGRIEPTA
                     QALLQNSTFVYK"
     gene            2286..2723
                     /locus_tag="VF222_12765"
     CDS             2286..2723
                     /locus_tag="VF222_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7143339.1"
                     /translation="MNINEIMSHVEINKTIIQRYFEAYNNKNEAIFDEIISPDYVDHG
                     QTSYMGSPGRGVAGAKNDLKYSLDRLDEFNYVVEAMIASEIYPDLVGTYWKGSLTPKS
                     TSDTQQTNKIINYRGVSIHRIQNGKMVETWHVVDGLPSVRLTR"
BASE COUNT         1070 a          484 c          448 g         1053 t
ORIGIN      
        1 ttcttgctgt tgtcgttttt gctgctttta cttgtgacat taatctttag ttaaatgggt
       61 cctaaaatca tgtcaaattt gtttcacatc ccactggccc cattgaactc tgggtgccaa
      121 tccgattttc agtagaaatg ccatcgtggt tattatccat tttatttaaa tagtataatt
      181 caaattgtta ttctgattat attcttaaga actattattt ctcatatagt caggaattaa
      241 acatcgcaat atattttatg atattgtcaa agaatatgaa gatataaaat aaaaaaaata
      301 agagagacaa gttatttgta cacaaacgtg ctattttgca acaatgcctg tgcagtaggt
      361 tctatccttc cagcctgaga catagcaaag caatgagttg acataaagtc ctgtgctcct
      421 ccaagtttat tttcccattt tggtaacata tcttgtacac atttttcgtt gacagcatca
      481 atatactttg aaaaaattaa ccaaggatta tcaaaaattc ctacttgttt tgcaatatca
      541 ctaaaaagga tttcattgtt agattttcca gctgtctttt ccaagtcaga gataaattct
      601 ttttgtacta ttacatcgtt aattgttcca agtctagtaa gatgtccccc aacaaacgta
      661 tcaaaatcat aattgttaag gactatgtca tgtgctttga tataacccgc tatatcttta
      721 gatactgcaa gatatggaaa tggtacccag cctggaaata ttatatcaac tagcataagc
      781 accttttgtt ttggagcata gatgaatatg ttacccgggt catgattatc gccataatag
      841 tctagttgaa gggtttggtt tccaacctgc agagtataat tatttgagaa ggttttagta
      901 ggtattggcg gaaccataga agcatttgtg gccaccgttt tagctctttg taattccgca
      961 gcagtttcct cctgagctat aataacagca ttttttggaa acattcctgc agcaccaata
     1021 tggtctatat gggcatgact ataaattaca tgggtaatag gtttgtcagt aacctctgat
     1081 atagctttaa gataattttt accaatagta ggaggagcat caactgctac aacaccttga
     1141 tctgttacta gaaatattgt attataagca ccttctgtta cccaaaataa cccatcatgg
     1201 atttcttcaa ctagatatcc tttcgaagga atagcaggac ccttcgcagc ttcaggtact
     1261 tggattgttg tttctgtttg attttcttgt gcataaatag atgtaaataa tgatatattg
     1321 gcaattagaa ttgaggtcgt tgaaaataac aatgataata atagaaaacc taaatttttc
     1381 tctattttca ccttttattt ataaaaatat tagtatttaa acttatttta aataataatt
     1441 ttatcgatag attcatcact aaggatattt atcttaaaat tttttattaa aaattgctag
     1501 gcaaatatta tattttccaa aatttatttt atttttttta ataaaatatt tatttttatt
     1561 aagaattata aaaagtttct aataagctct aaatattata taaatagaac catactacat
     1621 aaaggcttcc actactgact atcttttttg cttttgtaag tgaacccgaa tgtcaaagaa
     1681 ggattgaatt atgttatgag atactctatt tactatcatt attactattt acaaagaatt
     1741 ttgaaagttg atctattaca aaatctggtt gttcttctgg aatccagtgt cccgaatatg
     1801 gaactattat accttgtaca ttagtagcta gagccagaga ttgtatagaa tttaacgtag
     1861 tgctcccaag aattaaccga aagtaaagga atagaattga aatccttttc ctttaacata
     1921 aattacaatg taatgagccg agtaattgtt atgtgtggct ataacatact aactacaaat
     1981 tattgtgagc aatataaaga taataataac aacgatagcg ctatcaaata tcttcaaccc
     2041 aataatgata acatattaga cttgccgaaa agcattaaga aaaccttgtt gaaccagtga
     2101 caaataatgc tataaatgtt tcttcttctt ccatcccata tcagtaaagc gaccagttac
     2161 aactgcaact tctcgttgta aaataggtat agtttgttag acacaattta atcatataaa
     2221 gatcatagct accaatggat aacttccaaa ttataataaa tgaataaaga gtttaatata
     2281 ttagtatgaa tattaatgag ataatgtccc acgttgaaat caacaaaaca attattcaac
     2341 gctactttga agcgtacaat aacaaaaatg aagcaatatt tgatgaaata atatcccccg
     2401 attacgtaga ccatggccaa acctcttata tgggctctcc tggaagagga gttgctggag
     2461 caaagaatga ccttaagtat tctttagata gattagatga attcaattat gtggtagaag
     2521 caatgattgc atcagaaatt tatccagatc ttgtaggcac atattggaaa ggttcattga
     2581 caccaaaatc tacctctgac actcagcaaa ctaataaaat aatcaactac agaggcgtaa
     2641 gtatacaccg cattcaaaat ggaaaaatgg tagaaacttg gcatgtcgta gatggtttac
     2701 catcggtgag attaacaagg taaacccaat atccacaacc catctagttt tattacgata
     2761 attatattct attatatatt taatagtaaa ataatctaat ataacatatt tttacattag
     2821 atattgttaa taaaatttta taactattaa cagaactttc ttagttcttt aacaaattca
     2881 ataattatca aacaaacatt attttgttat atcaaacctg tttatatgaa tatttaatat
     2941 aaagaaatta aatttgcaat ttttcataaa ttgtaattag gacttttaat tcaagatagg
     3001 accaaaaaat aggtcaaatt tgtttcacat cccactggcc ccattatata agcgg
//
LOCUS       DASVEV010000293         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1715806, whole genome shotgun sequence.
ACCESSION   DASVEV010000293 DASVEV010000000
VERSION     DASVEV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715806"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..683
                     /locus_tag="VF222_12770"
     CDS             369..683
                     /locus_tag="VF222_12770"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX7143340.1"
                     /translation="MRYNEKDKAEQIPGSQNAGETASVVSSQGGIPGQRKEVNVENYE
                     KVASLGQILKDFEFPTNKDKIMQFVKTNVTDKELLNKLEQLPDKEYKNVSEVTHEAGL
                     VY"
     gene            complement(1164..2180)
                     /locus_tag="VF222_12775"
     CDS             complement(1164..2180)
                     /locus_tag="VF222_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7143341.1"
                     /translation="MLKNLILISFLFTGIFSLTIPLSNLVNAQDSSLTNSPKTLKNFN
                     IAVASDWGCNEDAQKTADNIQSKNPELVIAGGDLSYKGSGACWTDIIAPFESKTTISM
                     GDHDYDDTDGGKAGAVSDYLKPLGMTNTYYSFNLNNVHIVAIDPYVAYGPNSAQYKFV
                     VNDLKTAYNNHKIDWIFVIEHIPLYSSPSKHPADATIRDVFHPLFDKYNVDLVFSGDN
                     HNYQRTYPLTYNTADSSTPIVTKKDHSNYKDDKSGVIYIISGTAGRSHYDIKDQSPFV
                     AKQDDTQFGFLNIDIKSDNALVGTFYANEKDPNYNPISKSNQIGNILDKFTISKFATV
                     GSLS"
     gene            complement(2767..4074)
                     /locus_tag="VF222_12780"
     CDS             complement(2767..4074)
                     /locus_tag="VF222_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7143342.1"
                     /translation="MSKCFFKISKNIAPSMLLLLTVYFIGLSIFCLNNNNYSFAIESQ
                     ASNNNDEFNIITAADFGCSIRAQENIKNIEKFNPELVLVPGDLSYKKTAACWINMTKP
                     LDSKIKIAIGNHEDYEEEGAKGEKLKKSLLDHYGLDKSYYSFDYNNVHILVLDTQLEL
                     SVDTLQSSAVVNETLLAKSAEEGKAEDKSDHKKENPLLERYPIINIKDFMEKNSINVE
                     DPALEELEEINAKVPDLQIDKQQYQFVLDDLKKTNQNKNIEWIFVMFHKPMYSSISKQ
                     LEEYIIRDKYQEIFDKYNVDLVIQGHNHIYSRTLPLSFNKQQISEPILDQTNIANSTN
                     NNTFTNPNGIIFLVVGTGGDELYEITEKPYYIQNQYDKGFGFVDLKIDGKRLDGTFYD
                     INLDCKMGITEKKKKEVLNLESCKPPLPGNDELKKIDHFTINK"
     gene            complement(4425..>5542)
                     /locus_tag="VF222_12785"
     CDS             complement(4425..>5542)
                     /locus_tag="VF222_12785"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX7143343.1"
                     /translation="SAAKANVTESAAKANVTESAAKANVTESAAKANVTESAAKANVT
                     ESAAKANVIKTPADKELKYCYDSQFGSLGTGDGQFNRPHDIVFDSKGFLYINDRELNN
                     FQKFSPDGKFISQFGEKGEEIGQYKSPYSMAMDSNDMIYVLDRGNDRVVKVATDGTVA
                     GAWYSYDGPLVTANDDIPKFDKKDKKDKNVKTSKYSNPEAMAIDKNGNFYLTDTGNNR
                     IIKLDKNFKYISQFGQEGKGPGQLDHPHGIGIDSKGNIYINGLNDPRIQKFTNDGKFI
                     KEWGTNGTGPGQFTLPLEHLKVDSKDRVFITDGAGNPRVQVFDSDGKFLTQFGKLGVG
                     NGEMKKPEHVAFDEKDEKGTVYVVDRGNHRIQIFLPC"
BASE COUNT         1770 a          858 c          776 g         2138 t
ORIGIN      
        1 tgtatttctc ttaattagta gtatagcaaa gaaaattagt ttttcattac taaccttatc
       61 caatcgtata atattgtaaa tgttacaatg agattaaatt atttatcaag gcaaaaccgt
      121 attgagattt ttaataattt attgtattaa tattattaag aattatagta ttatttcttg
      181 agatatatat tttgtcttgt tagtttataa ctttctttat taatgacaat acagtttttt
      241 tcagaagggt atatagagat gtatgtttat ttggttatta ctcatattaa gaatttatga
      301 attcatgatt tagaccttta tcttgtaaaa taatcaagca ataaatattt tgatgtacat
      361 accatattat gcgttataat gaaaaggata aggcagaaca gattccagga tctcaaaacg
      421 ctggtgaaac agcctccgta gtaagttcac aaggaggtat tccaggtcag agaaaagaag
      481 taaatgtaga aaattatgaa aaggttgcat cactaggtca aatacttaaa gattttgaat
      541 ttccaacaaa taaagataaa ataatgcaat ttgtaaaaac taatgttaca gataaagaac
      601 tattgaataa actagaacaa ctacctgata aggaatacaa aaatgtatca gaagttacac
      661 atgaggccgg actggtctac tagtagtagt tttttaatgg tatttaccta ctagattctt
      721 ttactttgtg tacactttga aatgatcgga ctgaaattta atttgattaa ttttttttaa
      781 tctaacaata tatacaaatt ataattattt ttctctctac aaatgcttgt tttatcagta
      841 atcagtgtaa tattaaagac tcgataacta cattttatta aatgtatatc gtaacagact
      901 aaataaatta ttatggctaa tacttataat gaatttcttg cctggttctc tttctcttaa
      961 caatggtatg atactagctc tccctttctt taataatggc taatgaatat tatatagcaa
     1021 caagtagtac aaagttcctt tatgctctaa ataaatgaaa aaaataatag atttgatttc
     1081 tcctacaatc aataaaagtt agataatttg atttggcaaa taaattaaaa cctctagctt
     1141 gatattatag gtgcgtttat ttcttaactt agtgatccca ctgttgcgaa ctttgagatt
     1201 gtaaatttgt ctaatatgtt accaatttga tttgatttgg atataggatt ataatttggg
     1261 tctttctcat tcgcataaaa agtcccaacc aaggcattgt cacttttgat atcaatattt
     1321 agaaaaccaa actgagtatc atcttgtttt gcaacaaaag gagattgatc tttaatatca
     1381 taatgagatc tacccgctgt accacttatt atataaatca ctccagattt gtcatcttta
     1441 taattactat gatctttctt tgttacgatg ggagttgaac tatcagcagt attatatgta
     1501 aggggatatg ttctctgata gttgtgatta tctccgctaa ataccaaatc aacattgtat
     1561 ttatcgaata aagggtgaaa tacatctctg attgtagcgt cagcaggatg tttagacggg
     1621 gaactataaa gtgggatatg ttctataaca aatatccagt ctatcttatg gttgttataa
     1681 gcagttttta aatcatttac aacgaattta tactgagctg aattaggacc gtacgcaaca
     1741 tacggatcaa tagcaactat atgaacatta tttaggttga aagaatagta tgtatttgtc
     1801 attcctagtg gttttaggta atcacttact gccccagctt tcccaccatc agtatcatca
     1861 taatcatgat ctcccattga tattgttgtt ttggactcga agggtgcaat tatatctgtc
     1921 cagcacgctc cagatccttt ataacttagg tcacctcctg caattaccag ctctgggttc
     1981 tttgactgga tattatccgc tgttttctgt gcatcctcgt tacatcccca atctgaggcc
     2041 actgcaatat tgaaattctt aagtgttttt ggactatttg tgagagatga atcttgagca
     2101 ttcacaagat tagagagagg tattgttaaa ctgaagattc ctgtaaagag gaaagatatc
     2161 aatataagat ttttcagcat ttacttcact atcctatttt tttataaagt ttttttgata
     2221 ttcagtattt tttcgaaata ataggattac taacagtact aattacatta tagaaatttg
     2281 acaggttttg aatttggtta tccaattttt caagataaag actaatttgc atgtataata
     2341 ataggtagtt tatctttaaa aggtatgtga tacaaatagg tagatgatag atatttacta
     2401 tataaagttt aagaagactt tatacatact tgtattaatc aatctataca tcatagtttt
     2461 tgtttatcaa ggtcaaggta aaatgaatat ataaggataa gtcagttatt tatttgtaga
     2521 tattttaaag aaattaattt atatagcaga attttaaagt agagaattat tagtaacaaa
     2581 atacaaattt atctttttgt aaagatgggt ttatttcttt aagacaattt caattttaat
     2641 aatcaaaaat tatattagct aactagatat tggatttacc ttttgaacct atttttatta
     2701 atggttaatt tctcttattt atttcaattt attactctat tatagtatat cttttaacta
     2761 cctcatctat ttatttatag taaaatgatc tatttttttt aattcatcat tacctggcaa
     2821 tggtggctta caggattcaa gatttagaac ttctttcttt ttcttctctg ttattcccat
     2881 tttgcaatct aaattgatat cataaaatgt tccatctaat cttttaccat ctattttaag
     2941 atctacaaaa ccaaatcctt tgtcatattg attttgtata taatacggtt tttcagttat
     3001 ttcataaagt tcatcccctc ctgttccaac aaccaaaaag ataatgccgt tgggattagt
     3061 aaatgtatta ttattggtac tattagcaat attagtctga tctaaaatag gctcagagat
     3121 ctgttgcttg ttgaaagaga gaggtagtgt tctgctataa atatggttat gaccttgaat
     3181 aacgagatct acattatatt tatcaaagat ttcttgatat ttatcccgta taatatattc
     3241 ctccaactgt ttagatatag aggaatacat tggtttatga aacattacaa atatccactc
     3301 tatattttta ttttggttag tttttttcaa atcatccagt acaaattggt attgttgttt
     3361 gtcgatctgt aaatctggaa cttttgcatt tatttcttca agctcttcaa gagctggatc
     3421 ttctacatta atcgaatttt tctccataaa atctttaata tttattattg gatatctctc
     3481 gagtagtgga ttttcctttt tatggtctga cttgtcttca gcttttcctt cctcagcaga
     3541 tttagctaat aatgtttcat ttactactgc tgatgattgt aatgtatcaa cagacaattc
     3601 taattgtgta tcgagtacta ggatatgaac attattataa tcaaatgagt aatatgattt
     3661 atctaagcca taatgatcca gtaaagattt ttttaatttt tcaccttttg ctccttcttc
     3721 ttcataatct tcatgattac ctattgctat tttaattttt gagtctaatg gtttggtcat
     3781 gttaatccaa catgcagctg tctttttata actaaggtca cctggaacaa gtactagttc
     3841 tggattaaat ttttctatat ttttaatatt ttcttgagct ctgattgaac atccaaagtc
     3901 agctgccgtt ataatattaa actcatcgtt attgttgcta gcttgacttt caattgcaaa
     3961 tgaataatta ttattattta agcaaaatat gcttaaacca ataaaataga cagttagtaa
     4021 taataacatt gatggtgcaa tatttttaga tattttaaaa aaacacttac tcattttcat
     4081 tcagcttttg tttgcttaag ataataggta tatttatatt aattcagata aattttttag
     4141 atatttttaa taatttaatt tcattcatta ttattcttga cacattttta tcgcccaggg
     4201 cttgtatttt gtattgaatt acctgttttt tgtatctgac gtgcatcccc aatctccaac
     4261 aattgcaaaa ttataatcag tatctatagc agaaacatta gcaaataaat caaactttaa
     4321 aaaatacaac atagttatga taacaatatt aaaaaaaatt gttaatggtt gttaagcctc
     4381 tttgtttatt tatattcaca actattcttg ttttatctat cttcttaaca aggtaagaat
     4441 atttgtattc tgtgatttcc tctgtctaca acatagacag tacccttctc atctttttca
     4501 tcaaatgcta catgttctgg tttcttcatc tccccattac ctactcccag tttaccaaac
     4561 tgtgtaagga attttccatc actatcaaaa acttgaacac gaggattacc tgctccatct
     4621 gttataaaca ctctatcttt tgaatcaact tttaaatgtt ctaaaggtaa tgtgaattga
     4681 cctggtcctg taccattagt tccccattct ttgataaatt taccatcatt tgtaaatttc
     4741 tgaatccgtg gatcatttaa tccattaatg tatatgtttc ccttagagtc tatacctatt
     4801 ccgtgaggat ggtctaattg acctggtcct ttgccttctt gaccaaattg tgaaatgtat
     4861 ttaaagttct tgtcaagttt aataattcta ttgttacctg tatccgtcaa ataaaagtta
     4921 ccgtttttgt caattgccat ggcttcggga ttagaatatt tacttgtttt aacattttta
     4981 tcttttttat cttttttatc gaattttggt atatcgtcat tagccgtgac taacggtcca
     5041 tcataggaat accatgcccc agccacagtt ccatctgtag ctacttttac aacacgatca
     5101 ttccccctgt ctaaaacata tatcatatca ttagaatcca ttgccatgct gtatggactt
     5161 ttatattgtc ctatttcttc acctttctct ccaaattgag aaataaattt accatcaggt
     5221 gagaactttt ggaaattgtt tagttctcga tcatttatat ataaaaagcc ttttgaatca
     5281 aaaacaatat catgtggtcg attaaattgg ccatctcctg tacctaaaga tccaaattga
     5341 gagtcataac aatattttaa ctccttatca gcgggggttt taataacatt agccttggca
     5401 gcactttcag taacattagc cttggcagca ctttcagtaa cattagcctt ggcagcactt
     5461 tcagtaacat tagccttggc agcactttca gtaacattag ccttggcagc actttcagta
     5521 acattagcct tggcagcact tt
//
LOCUS       DASVEV010000294        13281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1131809, whole genome shotgun sequence.
ACCESSION   DASVEV010000294 DASVEV010000000
VERSION     DASVEV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131809"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            600..947
                     /locus_tag="VF222_12790"
     CDS             600..947
                     /locus_tag="VF222_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143344.1"
                     /translation="MLVVDDEPDICLVLKLVLEKTGFLVDYYYNPILALDEFKPNVYD
                     LIILDIQMPDINGLQLYREIKKRDIKAKVCFLTASESIFDIAYKFSPVYTFIRKPVNN
                     TDLIKIVNDLLNT"
     gene            1415..1849
                     /locus_tag="VF222_12795"
     CDS             1415..1849
                     /locus_tag="VF222_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143345.1"
                     /translation="MDSKKNNNNNKKIMIIDDDKDITNLFAIFLEYNGYIVDAYTNPV
                     EAFNTFRKNSHDLIILDLKMPMMDGMTLYHKIKEIDNNVIICFTTADINNIEDLRKGI
                     IDIDKIVLYKPVLLKDLKNKIDSLLLLSPQDVNGNKPTMMIL"
     gene            complement(2927..3082)
                     /locus_tag="VF222_12800"
     CDS             complement(2927..3082)
                     /locus_tag="VF222_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143346.1"
                     /translation="MRIEKKEMAKKKTGKLIIAFDALLLTFFYIIDVGSLSIIKISHY
                     VFSQISK"
     gene            3982..5577
                     /locus_tag="VF222_12805"
     CDS             3982..5577
                     /locus_tag="VF222_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7143347.1"
                     /translation="MVNVESIKEERIKAKNKGVKFRYITEVTTKNISYCKNMIKYFGA
                     EIRHLDGVKGNFEISDDGEEYVATANLQEAKPLKQLIYSNVKEIGEQQQYIFDILWTK
                     TISFEEKIKEIEEGSIPEVTDIIRNPDKAQALEWNLLSMATKEIQIIYSTVKAYKMQD
                     KVGVIDYLAKLAAKNDGIKVRILTPNDSSIQESLKSLKDNTNVNINYIEPKSGIIINT
                     IIVDKNYTLVMELKDKDYSTEKENFTTIPKKTTAITEETTTEIGKSTYSNSKPTVLSY
                     VSVFETLWRQTELFEELKQVDILKTEFINIAAHELRTPTQTIIGYCEMIDVFPEKKDE
                     YIHTIKRNSERLYKLTQDILEVAKIESNSLKLNIEKIELNEIINKTVIDFIRRPLSIN
                     KDVKINFAPSNHHQSSLFVYADKNRIQQVIFNLLDNASKFTEKGEITISIIVEEKDGN
                     NNNNNNITIRIQDTGKGIDSEILPRLFKKFATKSERGTGLGLYISKNIVEAHGGKIWI
                     EKTSNDKGTVFVFSLPTRNNSQK"
     gene            5898..6341
                     /locus_tag="VF222_12810"
     CDS             5898..6341
                     /locus_tag="VF222_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143348.1"
                     /translation="MAVNTSKNSDDYKKEIVKPISITNNNNNNNAIDQPTTSEKVNPD
                     ERDTKESEMQGKSESIKDKTSGKISEFVEGTKSEDKGKGSSNGIKSIIFGLFALIGFI
                     IIVYSIFVGVVGEPLASVADTVFVAVIIGSFTLVGAIVGRIFLKK"
     gene            6778..6999
                     /locus_tag="VF222_12815"
     CDS             6778..6999
                     /locus_tag="VF222_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143349.1"
                     /translation="MSKKKKEIRIIKNDSNKEDYTFVSSDNILNNTKNNEYINNEIKS
                     GTKPNNRIEKKIHSKKVENKGRKILGKSM"
     gene            complement(7426..8043)
                     /locus_tag="VF222_12820"
     CDS             complement(7426..8043)
                     /locus_tag="VF222_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143350.1"
                     /translation="MVSGKYFNKGVKALDEPNLGHVVRETPDKIVVFGGKNKRYDIPI
                     SEIQQVGANVLIGRNLSEIESKYRVNRNDPLPTSRKDPWQSDNQIDLGTYEGKYPNSL
                     FNKGVRTENEDHVGHVMKETDDKIVVFGYGNERFDIPKSEIIAVGRNVIVKKNFPELF
                     TYKVDMNAPLPTGEPIEKIDEEAYPEYYHGPRDDKEDKQDLIYPK"
     gene            9027..9632
                     /locus_tag="VF222_12825"
     CDS             9027..9632
                     /locus_tag="VF222_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143351.1"
                     /translation="MSARKDEIKANENIAVNESKNTTKYNTDNNNFNERTNRDYNNTL
                     NQQQDAINKTFDNTLNNVKRTTDEATHEIPRYTQRIAEYQEQTIQTVKDIASDFIEAE
                     KQVIGSFQSQVDRNTNGVWDLYNPQRIAENHAKMVNNFTSYLLNTSNLINNALASNMK
                     VYNTSLEQTRDNLKACAKTNTNFIQSVSGNRQNREANSSSP"
     gene            9841..10152
                     /locus_tag="VF222_12830"
     CDS             9841..10152
                     /locus_tag="VF222_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143352.1"
                     /translation="MKVVQNGNKCLEIIHSSTEDNDNNDYDIFIVDTHLHDISGFEVA
                     RKIRNRLPHKRIILTTTYSLDSISNMIDSIGMKSQDVLFKPFSFSELVSILKEPGMSY
                     N"
     gene            complement(10561..10680)
                     /locus_tag="VF222_12835"
     CDS             complement(10561..10680)
                     /locus_tag="VF222_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7143353.1"
                     /translation="MPIITVSIYSGRTQREKDRLAEAITEDIAKILNIKKEGI"
     gene            11107..11361
                     /locus_tag="VF222_12840"
     CDS             11107..11361
                     /locus_tag="VF222_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143354.1"
                     /translation="MSDELRRILTIISDLTHNDTNSSVKDTKIIAEANRQSEEIEKDL
                     NELQSLGLITEDSSKPAGVDYGMYRITREGINEIYNKEFR"
     gene            12111..12767
                     /locus_tag="VF222_12845"
     CDS             12111..12767
                     /locus_tag="VF222_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143355.1"
                     /translation="MIKKTSKQNSKEKKIKNDTKNKKKQAVLKKTKSSNLNSKTISNK
                     PKPKKTSKVNSTKRKKDIILKRSSGREEKFDTNKLTQTVSRSGVSFPVARDVAKSITK
                     KIKKSAQNKSTGTTKKKQQQQQSSVVATKQQKLKINTNKKEAEKVIVTASQIKNLVDD
                     ELKDRNQQDHSPSFSGNTTIGEESSMKITLDDREPVMDKVAANKNKVLFDPSKQKGSS
                     "
     gene            13049..>13281
                     /locus_tag="VF222_12850"
     CDS             13049..>13281
                     /locus_tag="VF222_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7143356.1"
                     /translation="MGNRSRTEIISNILDSANGGASKTKIMYKSYLSYNQLKEYLSIL
                     IENNLIEYLDGTHQFKTTEKGLNLLKMHNEMAE"
BASE COUNT         5348 a         1713 c         1755 g         4465 t
ORIGIN      
        1 gtattgacct tatccatttt agcaatagga ataattttca tttatttagc tcataggata
       61 cgatagttct attggtagca ggaggaatta tctttttttg caaaagcaag atcttatttg
      121 atatttcaat agaactaaga aagaggagaa acaatagaat tattataata gaaaatgtca
      181 agttaagaac tcatcttata attagtaaat tagcataatg aatgatattt ttggtggaat
      241 gtttctaaag aattattttt gtttatcatg tagatagaac tagtacaaag ttgaaactta
      301 tctaaaaata atattatgga atgatttata atataataag atattcatat gatatattaa
      361 tagaatcaat gttacattct atagaattat tttatacttt aatatttata ctatacaatg
      421 gccttttcca tcatttcttg ttaattcttt ttcttcagtt tctactacaa tctattgatt
      481 gtagtatatt taacatatag aaagagttat tcaatatcta tgtgtaatta aattattata
      541 acaaataatt aatagtaatt ctatgcaata atattattaa cactgacaat aaaaaacgaa
      601 tattagtagt cgatgatgag ccagatattt gtttagtatt aaagctagtt ttagaaaaaa
      661 ctggattttt agtagattac tattataatc ctatactagc acttgatgaa ttcaaaccta
      721 acgtttatga tttaataata ctagatatac aaatgccaga tattaatgga ttacaattgt
      781 atagagaaat aaaaaaaagg gatataaagg caaaagtatg ttttctcact gcaagtgaat
      841 ccatatttga tattgcatac aagttttctc ctgtgtatac ttttattaga aaacctgtaa
      901 ataatacaga tttaataaaa atagttaatg acttattaaa tacctaataa agagcaagat
      961 tagtgcatca taaatctaag caatatatcc tagaaatatt ctatttcctt tatattgtaa
     1021 gttactaata ataaaagcta aatactagat atgagaattt ccattcataa tatgttcaga
     1081 ttccagtgac aagaataaat agaagattaa ggcttcatgt taatttaatt tttaaaaata
     1141 gtattggcaa taagggaatt gttctgatct tttaaaggat gttacagaac atgaacacaa
     1201 gttactatat aaagtagcat attaatattt atatttatat atgtatatat agaacgcatt
     1261 agagagaaat tataatacat ataacaaaac agaacatggt acaaagcaaa actccgtctt
     1321 accatctact gaaaatagaa tcttttgaat attcattaat aaaagtttca aattctaata
     1381 tagaatgttg aataataatt agagaattgg aatagtggat agtaaaaaaa ataataataa
     1441 taataaaaaa ataatgataa tagatgatga taaagacatc actaatttat ttgcaatatt
     1501 tttagaatac aatggctata ttgtagatgc atataccaat cctgttgaag cattcaatac
     1561 ctttagaaaa aacagtcacg atttaattat acttgatttg aaaatgccaa tgatggatgg
     1621 aatgacatta tatcacaaaa ttaaagaaat tgataataac gtaataattt gttttaccac
     1681 tgcagatata aacaatattg aagatttgcg caaaggcatt atcgatattg ataaaattgt
     1741 tttatacaaa ccagttttat tgaaagactt gaaaaataag attgactcct tattgttatt
     1801 atctccgcaa gacgtaaatg gtaataaacc aaccatgatg attctttgac atcacttact
     1861 ttttcataaa ttttttattt attaacttaa taataacaat aaaatgaatt gtatttgtaa
     1921 atgatgatat ggtaattatt taatagataa actacctgtg agaatggata tttaaaccca
     1981 tgcataagtt attcttccaa tggttttgta atggtaaaag taatattcat aatctataac
     2041 atttattatg gcttatgata tttatctaat tttattatta tcaaagagaa ataaaaaact
     2101 ataccaatat tataaaaaat aaattaacaa cattttttca gaattaaatt cataataaaa
     2161 ttaggccaag caaaatcaca ataaatatta tctattaaaa tatagactat gtgttagtat
     2221 cttaatcttc gaataccata tatatattat aaatgtctaa cgatatatgc acaagtcaac
     2281 acgaaccatc agccattttc taagaaggtt attgatatgt attgcgattg ataatgatag
     2341 tggtggaaag atagagtttg agcgataata aaataaacaa atgtcttaac tgtgattcgt
     2401 ctttaaaaga taattactac agtactaatt tttgttccgg acaatgcaaa gatgcttata
     2461 taagacgtac ataaaatact tgaaaaatat attaatattg acattattaa ttttttcaat
     2521 ttttataaat agtaagtcga tctaagacag atagattata attgcttagt atctatccaa
     2581 ttttttaact tctacttcta ctactgattc tatcgtttaa acatatgata ccataacgaa
     2641 aaccactgtt tcaccaaata taacttcaaa tgttagagaa caaagaaatt tgtggaatgg
     2701 atactcatta ttgtcttatc taggttaaaa ataaatttgt tatataacct acaggctcga
     2761 gttaaaaaca tgtattcagc tggaaatgta gcaatttatg ataaatgaaa atgaatgaca
     2821 accatattaa atcttgaaat tatcttcgaa ataatttata taaagaataa aataacaaaa
     2881 aagggctaaa tacaattaag taaattctca aaaatctaat taaattctat ttgcttattt
     2941 gggagaagac ataatgacta attttaatta tagataatga accaacatct atgatataaa
     3001 aaaaggttag taatagtgcg tcaaatgcaa taattaattt accagttttt ttcttagcca
     3061 tttctttttt ttctattctc acttgaatct ttgtggatta aaataaatat tatgtgataa
     3121 aaagatatca cgtattttgt tgcttcgtaa taaatatttt ttataataca tgataattgt
     3181 tgatcatcag ctgttaaaga taaaataaag tcatctataa tttttttaaa tttatgattc
     3241 tcataataat agatcattgt tatactttga tgttagcaat atacttttta aaagacatta
     3301 tagaaaatta gtggttatta tagatgatat ttgaaactat tatttattag ataacaataa
     3361 taattttcca acaatataga aattatacta taataaacaa aatcggtatt tcatcgagct
     3421 atgattcaaa ttatctacaa tatgtttatg caaatgaaaa atataggatt tattttctgt
     3481 accctcgatt tataaagtat taattgataa tggctttagg ctaaaaaagt aaaaaacaaa
     3541 aaatacaact tcaagaaatt gcgcatttgt gtaattatac ttctaaacag taggataaat
     3601 aaatctacta aaaatgtttt gccattccag tattaacaaa agttaagtta tttatttatt
     3661 acaggaatta ttttataata atgcttacat ttaagtatta taatgccctc tctataggta
     3721 gagaaaaaca agtgtatagt ataataatag taggaaagtg taagaataat tattcctact
     3781 gaagaaaata acaaccataa gcaagaaaaa aatgttataa ataataataa ccaaactttc
     3841 gacgaaggaa ataataaagg aaaaaaggaa aatactgaaa tctttcatgg tattgaagat
     3901 tcaaataatg caatattaaa tctcgtctat aaagcaaagt atagaattga tggctgtctg
     3961 gattcaaatg gaccatcagt aatggttaat gttgagtcaa ttaaagaaga aagaattaaa
     4021 gcaaaaaata aaggagtaaa atttagatac attaccgagg tcactacaaa aaatatttct
     4081 tattgtaaaa atatgataaa gtattttggt gcagaaatac gacatttaga tggcgttaaa
     4141 ggtaattttg agattagcga tgatggagaa gaatatgtag ccactgctaa tttacaagaa
     4201 gcaaagccct taaagcaact tatatatagc aatgtaaaag aaattggaga acaacaacaa
     4261 tatatttttg atattctttg gactaaaaca atctcttttg aagaaaagat aaaagaaatt
     4321 gaagaaggaa gtatacctga agttacagat atcattcgta atccggataa agcccaagcg
     4381 ttagagtgga atctattaag catggcaact aaagaaatac agataatata ttctactgtt
     4441 aaggcatata aaatgcaaga caaggtaggg gtaatagatt atttagctaa actggcagca
     4501 aaaaatgatg gtataaaggt gagaattctc actccgaatg attcgtcaat acaagaatca
     4561 ttaaagagtt tgaaagataa tactaatgtc aatattaatt atatagaacc taaatctgga
     4621 attataatta atactataat tgtagacaaa aattatactt tagtaatgga attaaaagat
     4681 aaggactatt cgacagaaaa agaaaacttt actactatcc ctaaaaaaac aacagcaata
     4741 acagaagaaa caacaacaga aataggaaaa tcaacctatt ccaatagtaa acctactgtc
     4801 ttatcatatg tatctgtatt tgaaacactg tggaggcaaa ctgaactatt tgaagaactt
     4861 aagcaagtag acattttgaa gacagaattt atcaatatag ctgctcatga acttagaacc
     4921 cctacacaaa ctattatagg atattgtgaa atgatagacg tatttcctga aaaaaaagat
     4981 gaatacatac acacaataaa aagaaattct gaaagattat acaaacttac acaggatata
     5041 ctagaggtag cgaaaataga aagtaattca ttaaagctaa atatagaaaa aattgaatta
     5101 aatgaaataa taaataaaac agtaatagat tttattcgta gacctttatc tattaacaaa
     5161 gatgtaaaaa taaatttcgc gccttctaat catcatcaat catcattatt tgtttatgca
     5221 gataaaaata gaatccagca agtaattttt aatctcttgg ataatgcttc taaatttaca
     5281 gagaaaggag aaataactat atcaataata gtagaagaaa aagatggaaa taataataat
     5341 aataataata taacaattag aatacaagat acgggtaaag gtatagactc tgaaatattg
     5401 ccacgactat tcaaaaaatt tgcaacaaaa tctgaaagag gaacaggtct tggtttgtac
     5461 atttccaaaa atattgtgga agctcatggt ggtaagatat ggattgaaaa gacttcaaat
     5521 gataaaggaa cagtatttgt atttagttta ccaacaagaa ataatagtca aaagtaattt
     5581 taaaataagt ttctagtaat aaaaactaaa tacaaggtag taacaggaca actgtactac
     5641 ataaaatttc ttttataaat tacatattaa aggcgttatt cactgtgtat ggtaattctt
     5701 tgattactat atttaagaaa agtaaatatg ataacaaaat caattaatca taggtattgt
     5761 atatttcaaa aatataggat agaaaaagga gaaattgctg aagaaactct ccacacttat
     5821 agaaacagat attaataaag tagattgtaa taattttaaa aattgttttt atacgattaa
     5881 atagaataat atttgaaatg gctgttaata cctctaaaaa ttctgatgat tataaaaaag
     5941 aaatagttaa accaatatct attactaata ataataataa taataatgca atagatcaac
     6001 ctactacttc tgaaaaggtt aatcctgatg aaagagatac taaagaatca gaaatgcaag
     6061 gaaaaagtga atctattaag gataaaacat ctggaaaaat atcagaattt gtagaaggaa
     6121 ccaaaagtga agataaagga aaaggatcat caaatggtat taaatcaata atttttggac
     6181 tatttgcttt gattggattt attatcattg tatattcgat atttgtagga gttgtaggag
     6241 aaccattagc atcagtagca gatactgtat ttgttgctgt aattattggg tcttttactt
     6301 tggtaggagc tattgttggt cgtatatttt taaagaaata ataccactat tttattttat
     6361 ttttcaagac atgaatggtc aatcttaggt tcatgctata ctatttcatc catttcaaat
     6421 atattaagtt actaaaaaac taaatacaag ataatagtat ctctacaata gacttttatt
     6481 ttagaaccgc tatcggtgtt actccctcct tcacaatttt ctaacaaatt cgcatatctc
     6541 gaaactttca aaaataattt taaaaattag atttactttg ttagttagct taaatttata
     6601 ttaatatctt gatacaaaaa gtattgtata ttatcaatcc ctgatataac atgcttttct
     6661 attgggtatt ataatttaat gttaattcaa aattatcaac actcactaat tcattcctca
     6721 tttattttgc aataacatta attgataact tgcatatata taaaataggt taaagaaatg
     6781 agtaagaaaa agaaggaaat taggataata aaaaatgatt ctaataagga agattatact
     6841 tttgttagca gcgataacat tctaaataat acaaaaaata atgaatatat aaataatgaa
     6901 attaagtctg ggaccaaacc aaataataga attgaaaaaa agattcactc aaagaaagta
     6961 gaaaacaaag gaagaaagat tctaggaaaa agtatgtaag gtcattcgcc agtatgaata
     7021 atcaccattg cagaatataa gttaccaaaa agctaaatac tctgtagtag aaccctacta
     7081 catgcaaaac tctactccat attattttat acagatttag aattatattt aagatggatt
     7141 ttataattaa tacgtttttg tcacgcatcg acttcatact agtaaagtct atttgacttt
     7201 catctaaatc caagaaagat tttgcaattt ttctaataga atctattgcc tgattttaaa
     7261 gtctttatca taacaggtta atagatagat agatatgtag cagatggatt atgataaaaa
     7321 ccttctattt tcaatcatga agaaataatc aaaaaaaaga gataggaata aataatactt
     7381 tttaggaaat ttaaaaagtt aatcctgatt ataatataac agattttatt ttggatatat
     7441 taaatcttgt ttatcctctt tatcatctct aggaccatgg taatattccg gatatgcttc
     7501 ttcatctatt ttttctatgg gttctccagt tggtaatggt gcattcatat ccaccttata
     7561 agtaaataat tctggaaagt tctttttcac aataacattc cttcctactg ctattatctc
     7621 agatttaggt atatcaaatc tctcatttcc atatccaaag actactattt tatcatctgt
     7681 ttccttcatg acatgcccaa catgatcttc gttttctgtt cttactcctt tattaaataa
     7741 tgagtttgga tacttgcctt cataggttcc caaatcaatt tgattatccg attgccaagg
     7801 atctttccgg ctggttggaa gtggatcatt acgatttact ctatatttac tttcaatctc
     7861 tgacaagtta cgtccaataa gaacattggc gccaacctgt tgaatttctg aaataggtat
     7921 atcatacctc ttattttttc caccaaatac tacaattttg tctggtgttt ccctaactac
     7981 gtgtcctaag ttaggttcat ctaatgcctt tactcctttg ttaaagtatt ttccagatac
     8041 caatagaaat atattgtaat aattctaaat aaaggatttg atattataag taatttttgt
     8101 tgaattttga tcctattgtg ttagtttgta attaaaaaat aatcaataaa ggatttatac
     8161 tatgaaattt taatactaaa tatagaatat attagaggca aaaatacctg gcaacccgat
     8221 agacctgtta tctattacca tcaaaataaa atagcgtcta ttaacaaaat tataggtttt
     8281 gtcttttaat agctaattct atttgtaata ttgaacattg aaactgtgtt tattcaactc
     8341 cagacgggtc cgtagcaata tgcctctatt tatagttcct tataaataga taatatcaag
     8401 actaagataa tgctcaaggc agcaacaaaa tctagcgata taatttagca ggaaaaattt
     8461 cctccttaaa gaatgataaa tatcatttta ttattgttga tatctcttag ttatttcact
     8521 ttaatggttg atgtaatctt ctttagcctt tgtttgtatc ttagagcatg attataaatt
     8581 ataattggat atttaggatg gagttcattc aatttatcat agcattctat ttctccttta
     8641 cattttaatt catatggaat gctattccac aaactatcgt tgtcatgcat aaatttaatt
     8701 agttttgatt gccttaaatg tactagtcat aaaccatgta tatagctagt attttttgtt
     8761 gagcatttag aactatatac tgaattaata ctgtagaata gtatgttgaa ctatatttac
     8821 cgcacctata tttgaaggtt catccacttc attttattat tatctatctt ctatgcctcc
     8881 tctagttgtt gttcttggaa ttatgaatat aatacctcaa attgaacata taaataaatt
     8941 aattgattga ttgtgaataa acaaatatga ctatacaata gacattaata catacgctta
     9001 tataccttag taacgtatat atatacatgt cagcaagaaa agacgaaata aaagcaaatg
     9061 aaaatatagc tgtaaatgaa tcaaaaaata caactaaata taacacagac aacaataatt
     9121 tcaatgaaag aactaataga gactacaata acaccttaaa tcaacaacaa gatgcaataa
     9181 acaagacatt tgacaacacc ttaaataatg taaaaagaac tactgatgaa gctacacacg
     9241 agattccacg atacacacaa aggattgctg aatatcaaga acaaactatt caaactgtaa
     9301 aagatattgc ttctgacttt attgaagcag aaaaacaagt aataggctct tttcaatcac
     9361 aagtagatag aaataccaat ggagtatggg atttgtataa tccacaaaga atcgcagaaa
     9421 atcatgcaaa aatggtaaat aactttacaa gttacctgtt aaatactagc aatctaataa
     9481 acaatgctct agcatcaaat atgaaagtat acaatacttc actagaacaa acacgtgata
     9541 atctaaaggc ttgtgcaaaa acaaacacca attttataca atcagtatca ggtaatagac
     9601 aaaatagaga ggcaaatagt agcagtcctt aacaaccacc ttaattacag gatacaaaag
     9661 ctaagctgct gccgtatcct tacgacgcct acaataatga ctgatttaaa ccaatccaca
     9721 gcagaaaacc aaaagaagaa agaaaaaaaa cctaaaagaa tccttatagc cgaaccagat
     9781 tcagatattt aatatcttta ttctcttttt acaaaacagt atggtttttc aatatcagat
     9841 gtgaaggttg tacaaaatgg caacaaatgc ttagaaatta tacattctag tacagaagac
     9901 aacgacaaca acgactatga tatttttata gtagacacgc atcttcatga catttcaggc
     9961 tttgaagttg caagaaagat tcgtaataga ttgccacata aaagaataat attaactaca
    10021 acctattcat tagatagtat aagcaatatg atagattcaa ttggtatgaa aagccaagat
    10081 gtgcttttta aaccttttag tttttctgaa ttagtttcta ttttaaaaga accagggatg
    10141 tcatacaact aagataacca taaaaactga gttatcattt aacatatatt aaataagttc
    10201 caactaaagc acttacaatt atattggtaa aaccaatgtt acatacacac atatcctgtt
    10261 gctaaaactt atcttctctc tatttaaatt gattcttaat tttaagaata ggtatctaga
    10321 aatgtaaaac tagataacta catcattaaa actagcaaaa taaaactaga gagaatcttt
    10381 taataatttg aatcagaatt attaaaaata ataattatta ttctctaata tttactatca
    10441 tggttattat ttttctaatt caaatgaatt tgttactatt atcatctacg tgacaaatat
    10501 agttatatta tagatgaact cttgaggaat accaatttcc gtgggatgct tcttgaaata
    10561 ctatatccct tctttcttta tgtttaatat cttagctatg tcttccgtga ttgcttctgc
    10621 aagtctatct ttttctctct gtgttctacc actatatatt gatactgtta ttattggcat
    10681 tgtataatta gataaatacc agagagatag ataagaatac ggttaactaa acttcactag
    10741 attagcggaa aagtttttcc atattatctt ggtaaaataa ttaatttttg tctgatttgc
    10801 taaatcctat aataacaatt tattataata gtttcttatc ttttatttaa ataagtttct
    10861 aataattaaa gctaaagaca aggaagtagg tgtatctctt caatagactt tattttagaa
    10921 ccgctatcgg tattaatgcc gacaaattaa ttacaaaaat tatacctatt ctaaattcat
    10981 attttgatta ttatatcagt aattataatc tacttttacc attagaatgt ctataaataa
    11041 gtaattaggt gatttttagc cttattttaa tttatattaa gttgttaatc aagatatgtt
    11101 atgtctatga gcgatgaatt aagaagaatt ttgactatta tcagtgattt aactcataat
    11161 gatacaaatt cgagtgtaaa agatactaaa ataattgcag aagctaatcg tcaatctgaa
    11221 gaaatcgaga aagacctcaa tgaattacaa tcacttggct taataacgga agatagctcc
    11281 aagcctgctg gtgtagatta tggaatgtat aggataacta gagaaggcat aaacgaaata
    11341 tacaataaag aatttcgata gcgatagtat cgaattatat aattagaaat atatcccaag
    11401 gaggtgcatt aggttatatt acaaccacct aacaaatact actttcaacc aataacgaca
    11461 gaagttacaa caacatatac aatttattaa cagatgttag attaattata ttattattta
    11521 ctacttacaa aaacaattta aattaagttc taatgataaa agctaaatac aagatagtgg
    11581 tagaacccta ctacatgcag gactttactc ccacaaactt ccataatata tggatgaatc
    11641 catttgttat taaaagataa ttcagattta agcaaagttg tcttaatctc tatttgttgt
    11701 tatacccact ttaagagcat ctttaacctt tttggtaaat tctaaagtag tttctgttgt
    11761 atagctattg tatttatgat taatcaagaa tctgtattta taacaataat gttattattt
    11821 agattacttt ttttttagcc atcataaaga ttttcaatta tctatcttat tctgtctttc
    11881 aatttctatt gaaagatcaa taataacctt tgaccaagag gatgttctgt taaatgattt
    11941 attgcagcct ctatgcatct gttatttctc tattagatga agatcaattt atcctaaatt
    12001 aagagcctaa catttagtaa tatatcgaat gagttaaatt aatataaatt acgaagtgtt
    12061 aaaaatactt aaatttgtat tgcttattat atatatagta ataatataag atgataaaaa
    12121 aaacgtctaa acaaaacagt aaagaaaaaa aaattaaaaa tgataccaaa aataaaaaga
    12181 aacaagcagt actaaagaaa actaaatctt ccaatttaaa ttctaaaact atttcgaata
    12241 aacctaaacc taaaaaaacc agtaaggtga attcaacgaa aagaaaaaaa gatataattt
    12301 taaaaagatc aagtggaaga gaggagaaat ttgatacaaa taagcttact caaactgtaa
    12361 gtagatcagg tgtttccttt ccagttgcaa gagatgtagc aaaatcaata acaaagaaaa
    12421 ttaaaaaatc agctcaaaat aaatcaactg gaacaacaaa gaaaaaacaa caacaacaac
    12481 aatcttcagt agtagcaact aaacaacaaa aattaaaaat taatacaaac aaaaaagaag
    12541 cagaaaaagt gattgttact gcaagtcaaa taaaaaattt ggtagatgat gaattaaagg
    12601 atagaaatca acaagatcat agcccatcat tttctggcaa tactactata ggtgaagaat
    12661 catccatgaa aataacactt gatgatagag aaccagtaat ggataaagtt gcagcgaata
    12721 agaataaagt tctctttgat cccagtaaac aaaaaggaag ttcttaaaaa aaccttcttg
    12781 tctttctata tcttttttta aatttcaaaa ttaatttctt tattatgtta tatacctaat
    12841 aagttgaaat attaactata atatgagact cttatttcgc acagctaaat actaacaata
    12901 attattttat ggtacaattt tgagctaaga cctgattttt cttttattat ttttatttgc
    12961 aaataatatg tggaatgttt tactataatt atcttaactt ttcttgtgat attaatacta
    13021 aaataagtaa ataacttatt taataaatat gggaaataga agccgaacag aaataattag
    13081 taatatactc gactcagcta atggaggagc gtccaagaca aaaattatgt ataaatctta
    13141 tctaagttat aaccaattaa aggaatacct ttctattttg atagaaaaca atctaataga
    13201 atatcttgat gggactcacc aattcaagac tacagaaaaa ggattaaact tacttaaaat
    13261 gcataacgaa atggcagaat t
//
LOCUS       DASVEV010000295         5839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1131837, whole genome shotgun sequence.
ACCESSION   DASVEV010000295 DASVEV010000000
VERSION     DASVEV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131837"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VF222_12855"
     CDS             <1..1650
                     /locus_tag="VF222_12855"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7143357.1"
                     /translation="STAAAISQLKFPFSLKEDQIDAVNAWIDNDFRGTIVYSTGTGKT
                     EIAFECAKRVACAYPSSSTLNILLMVPRIILIEQNTRRLVSYGIPKDKIGKYFGERKQ
                     IREITICTYQSIIRNLDIIRRSNMVIFDEIHLIRDTAKIFSKIFDVVIEDHKKAILGL
                     TATLDEKDLSKYNTILTLLPPVKRYPIKNAVNDKRLAKPIVIPLKVNLTEKEQKDYDT
                     YSTKIKRISNRFKKYDAESMTLLLRKGGFVSGMAKAWFLNVRKRKLLLSCAENKLLSA
                     LNLITKKFPNEKIMVFSETIDSITKLRDMLELEGTKAMIIDSNINSRKRQLILNQWGI
                     EFYPLLSVHTLEIGFDIPQVRIEVILATTSNMNQVIQRIGRVIRKQEGKDVALIYVIY
                     VSDTKDDKIHEIVKKAITTSAKSLQEYKEEEEEEDNRNKKIEIQKGILNNNNGNLKRL
                     KEVERKRLERAYDIIESTLYEPMIVELKEKEEEEKQDSDQDEENQDKREIRKLFRVKS
                     SIKEKIKYYDVDIEKKTCTCPDFKFKLHKCKHIVATELFLF"
     gene            complement(1859..2110)
                     /locus_tag="VF222_12860"
     CDS             complement(1859..2110)
                     /locus_tag="VF222_12860"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX7143358.1"
                     /translation="MPIFLDVHKVPFKQDHLKELVDLPTDEFGVRHVNLMYNKENGVC
                     FCLLDAPDHEAVVKHHDKVNIQCEWIVEVDLAKDQLSKQ"
     gene            2215..3330
                     /locus_tag="VF222_12865"
     CDS             2215..3330
                     /locus_tag="VF222_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143359.1"
                     /translation="MESVGNEFFPELKYEHHSGKALNQMSEIISRTTKNLESVFDHDW
                     IHILVENPEFFDTIVDFKIKQKGVSIRFITNITSENVLYCTKIMKFVELRHADTVTGY
                     LGISDKNQFFSYTEPISKQKNDIEEKNNDNLKLKFIHITNKKFVQMQYYFFEKLWKLS
                     IPASEKIAEIQRVMFDTSLLNTNITDLSTIEEIFPKVIQSAIEEVLLFFPTIDSFWFA
                     DRNGIIKLLGEALTRFIKVKVIIHINGEISNIKKEIERKVNETNKILGTYVNYTTKKI
                     DTNTIILVTDQAISFSISIKYSERGTFKDSIEMASFSNNELTVSALLSFFNSLWIRSD
                     IEKQNIIKQTYFKIFKEPQLKDENYRRKWFFEPTKKE"
     gene            complement(3446..3652)
                     /locus_tag="VF222_12870"
     CDS             complement(3446..3652)
                     /locus_tag="VF222_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143360.1"
                     /translation="MSEEKQIEDLKSQFRRTTDSELRKQMLDTISAYENNGIDAINDL
                     ISSTIDDEVKSYGLNLIKKIKQNP"
     gene            complement(4011..4238)
                     /locus_tag="VF222_12875"
     CDS             complement(4011..4238)
                     /locus_tag="VF222_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143361.1"
                     /translation="MSNNTKENSINIPSPFSFKYVESGGLANNYLVISFDPESNHLKV
                     STDISGTDLTQKSIEDSEKNDLISTITKKFF"
     gene            complement(4903..5037)
                     /locus_tag="VF222_12880"
     CDS             complement(4903..5037)
                     /locus_tag="VF222_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143362.1"
                     /translation="MGTSLILQKYREMMMLAAAAATIVHTVRHTIKKLPKCMMLLLKN
                     "
     gene            5507..>5839
                     /locus_tag="VF222_12885"
     CDS             5507..>5839
                     /locus_tag="VF222_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143363.1"
                     /translation="MSARKEEIKTNENIDVNKPTFYDKNTTKYSTDNTNTTNFNERIN
                     RDYNNTLNQQQDAINKTLDNTLNNVKRTTDEATREIPRYTQRIAEYQEQTIQTVKDIA
                     SDFIEAEKE"
BASE COUNT         2236 a          804 c          838 g         1961 t
ORIGIN      
        1 tcaaccgccg ccgccatttc tcaactaaaa ttcccttttt cactcaagga agatcaaatc
       61 gatgcagtaa acgcatggat tgataatgat tttagaggta caatagtata tagcactgga
      121 actggtaaaa ctgaaatagc ttttgaatgt gcaaagagag ttgcttgtgc ctatccttct
      181 tcttctactt tgaatattct tttaatggta cctaggatta ttttaatcga acagaacacc
      241 agacgacttg tatcatacgg tataccgaag gataaaatag gaaaatattt tggtgaaaga
      301 aagcagatta gagagataac gatatgtaca tatcaaagta ttattagaaa tctagacatt
      361 atccgtagat ccaatatggt tatttttgat gaaatacatc tgataagaga tacagcaaaa
      421 atatttagta aaatatttga tgtagtaata gaagatcata aaaaagctat tcttggcctt
      481 actgcaactc ttgatgaaaa agatctttct aaatacaata caattcttac attattgcca
      541 ccagttaaaa gataccctat caaaaatgct gttaatgata aaagacttgc aaagccgata
      601 gttattcctt taaaggtcaa ccttacagag aaggaacaaa aagactatga tacatactcc
      661 accaaaataa agagaatttc taaccgtttt aaaaaatatg atgctgagtc tatgacatta
      721 ttgctaagaa aaggcggttt tgttagtgga atggcaaaag catggtttct aaacgtaaga
      781 aaaaggaaat tattgttaag ttgcgcagaa aataaattgt tatctgcttt aaatctaata
      841 actaaaaaat ttccaaatga aaaaattatg gtatttagtg aaaccattga ttctatcact
      901 aaacttagag atatgttaga attagaaggc acaaaagcga tgataattga tagtaacata
      961 aattctagaa agagacaatt gatcttgaat caatggggaa tagaatttta tcctctctta
     1021 tcagtacata ccttggaaat aggatttgat ataccacaag ttagaataga agttatttta
     1081 gctacaactt ccaatatgaa tcaggtaata cagagaattg gtagagttat acgaaagcag
     1141 gaaggtaaag atgtggcatt aatctatgta atatatgtat cagacactaa agatgataaa
     1201 atacatgaaa tagtgaaaaa ggcaataaca acatcagcaa aaagtttaca agaatataaa
     1261 gaagaagaag aagaagaaga taatagaaat aagaaaatag aaatacaaaa aggtattctt
     1321 aataataata acggaaattt aaaaaggtta aaagaagttg aaagaaaaag acttgaaagg
     1381 gcttacgaca taatagaatc tactctatat gaacctatga tagttgaatt aaaagaaaaa
     1441 gaagaagaag aaaaacaaga tagtgaccaa gatgaagaaa atcaagataa aagagaaatt
     1501 agaaaattat ttagggttaa aagtagtatc aaggaaaaaa tcaaatacta tgatgttgat
     1561 atagaaaaga agacatgtac atgtccagat tttaaattta agctacacaa atgtaaacat
     1621 attgttgcaa cagaactatt cttgttttaa ccaatgagaa tataggagta gaaattataa
     1681 attagaattt ataattaagt tgagatatga taaagaatat tattttagaa aattgtcttt
     1741 ctttagtatt caaaaaaaag ttattcccca aatattaata ggatcttaga tgacacaaaa
     1801 atatatatta caaagaagca attataagag tttcatttat aaaaatattt ctattttctt
     1861 actgtttaga taattgatct ttagcaagat caacttctac aatccattca cactgtatat
     1921 tgactttgtc atgatgtttt acaactgctt catggtctgg tgcatccaat agacaaaagc
     1981 aaacaccatt ctctttgtta tacataagat ttacatgtct aactccaaat tcatctgttg
     2041 gtaaatctac caattcctta agatgatctt gtttaaatgg tactttatga acatccaaaa
     2101 atattggcat aatttcaata aaagttcata ttatttaaat gatttcaaaa tgcttgaaaa
     2161 ataaaaaatt attaatatct aaatttttat atctaaaaac aataattata gttagtggaa
     2221 tcagttggta atgaattttt cccagagtta aaatatgaac accattctgg aaaagcatta
     2281 aatcaaatgt ctgagattat ctctcggact acaaaaaatt tagaatctgt atttgatcat
     2341 gattggattc atattttagt tgaaaaccct gagttttttg ataccatagt tgatttcaag
     2401 atcaaacaaa aaggtgtgtc tattagattt attacaaata ttacatctga aaacgtactt
     2461 tactgtacta aaataatgaa atttgtggaa ttgagacatg cagataccgt tacaggttat
     2521 ttagggatat ctgacaaaaa tcagttcttt agttatactg agcctatttc caaacaaaag
     2581 aacgacatag aggagaaaaa caatgataat ttaaaattaa aatttattca tataaccaat
     2641 aaaaaatttg tgcaaatgca atactatttt tttgagaaac tttggaaatt atctattcca
     2701 gcaagtgaaa aaattgctga aattcaaaga gtaatgtttg atactagttt attaaatact
     2761 aatataacag atttaagtac aatcgaggaa atctttccca aagttattca gtctgcaata
     2821 gaagaggttc ttttattctt tccaacaatt gattcatttt ggtttgctga tcgtaacgga
     2881 attataaaat tgttaggaga agcacttact agatttatta aagtcaaagt aataattcat
     2941 attaatggtg agatttcaaa cattaagaaa gaaattgaac gaaaggttaa tgagacaaac
     3001 aagatattag gaacatatgt taattacact acaaaaaaga tagataccaa cactataata
     3061 cttgtaactg atcaagcaat ttcattttca atatctatta agtacagtga aagaggaaca
     3121 tttaaagact caatagaaat ggcctcattt tctaacaatg aattaactgt ttctgcatta
     3181 ctctcatttt tcaatagttt atggataaga tcagatattg aaaaacaaaa tatcataaaa
     3241 caaacatact ttaaaatatt taaagaacct caactgaaag atgaaaatta tagacgcaaa
     3301 tggttttttg aacctacaaa aaaagaataa aattgggggg agtcctgcag cgtttatcca
     3361 ggcaagcttt gaattggttg ttgttgatct taaaacgatc ccaggttcat taagttgtct
     3421 cactgatggt tgataaaaaa taaagttaag ggttctgttt tattttctta attaaattta
     3481 gcccgtaaga ttttacctca tcgtcaattg tgctgctgat caaatcgttt attgcatcta
     3541 taccattgtt ttcgtaagca gatattgtat caagcatttg ctttcgaagt tctgaatcag
     3601 tagttctgcg aaattggctt ttgaggtcct cgatctgttt ttcttctgac atattcgtta
     3661 ctcttatttt tgcaataagt cttatatctt ttaatgttat ttcaaatttt aatacagtat
     3721 ttaggcatta ttttaaatat ttgacttaat tctccgcagc agccttcccc tcttttgaaa
     3781 ataaaactag acattgaaat acgacgctat ataatgaatt ttaaatcatt tttttttcat
     3841 gtgctttatt ttttatttca tttgtaattt caaccaattc agcctggact tcttgagaat
     3901 tattggtcca tacagcagtg tgtatatctg tatttattgt aactgtaaga gaagatgaga
     3961 ttgcagtatt atcttaattt tcattttctg taacatacgt agcttttgta ttaaaaaaat
     4021 ttttttgtaa ttgtacttat taaatcattt ttttctgaat cttctataga tttttgggtt
     4081 aaatcagtac cactaatatc cgtggatact ttaaggtgat tagattctgg atcaaaagaa
     4141 ataactaaat aattgtttgc taatcctccc gattctacat atttaaagct aaaaggcgaa
     4201 ggaatattta ttgaattttc tttagtatta ttagacatag attattaaca tcatagcatt
     4261 aacatattat ttaataattc taatcgattg agagataagt ggttgattaa aatagcagat
     4321 gttggatatt gtttgaccta tcagattcaa tgtatagaat ccttctaata tgcttcgtca
     4381 cctctgtctt taaactagca cttatcttta tcaccattct attctccatt ataatattga
     4441 caatatttct tctagtcgtt atatttttac ataacctctt gcattaatgg gttatagaga
     4501 ttttaatgct atcactactg aatcattact aataaatgat gctttcagga tattaatatg
     4561 aaatgagatt atagaatata attgttcaac cagatataat aaatatttta tacataaatt
     4621 ataactttat cccctccgaa attaacccct agagtgatat tgaataagat tctaaattat
     4681 agtaatttaa atagtataga ctatttttta aaatacaaaa ataaccttta agtgaaccca
     4741 ataaatatag tggtagattt gaacctttac tgtttataac tttgttttgc taggtgttcc
     4801 ccaatttatt gtaattctct tacaaatgta tcatagtcat agttttggta taaagaacat
     4861 tgctccaaaa tgatgtttgt ccaataataa ctgcgaggtc attcaattct tcaacagtag
     4921 catcatacat tttggcaact tctttatggt atgtctgaca gtatgaacaa ttgtagcggc
     4981 tgctgcagct aacatcatca tttctctata tttttgtagt attaaagatg tgcccatctg
     5041 atatttctta aacaaaggcc acatttgtgg aagaatatct ttgtgtattt ttcataaatc
     5101 ctgtcaacat actttaattt acattcaatt tcatgctttt attagatgta gatataaaat
     5161 cacaaaccac gtaatacaac ttttagaaga atcgacttaa ttaatgatat gcagttaaat
     5221 attttctaga tctattcttg atattctctg atttttcttt aaaagcttct cctattgtca
     5281 tattttatct catgatcaat tactgccgaa tgttccttaa cacataacat ttacaagacg
     5341 gcttgtatat gtcacatcgt gaaacaacaa tagtcaaatt atcatattcg tttaatacct
     5401 tgaggtaccc tgaattaaac aaataaggag tttataacta tataatatga ctattcatta
     5461 gtcattaata catacgttta tatatcatag taacgtatat atatacatgt cagcaagaaa
     5521 agaagaaata aaaactaatg aaaatataga tgtaaataaa ccaacttttt atgataaaaa
     5581 tacaactaaa tatagcacag acaacaccaa taccactaat ttcaatgaaa gaataaatag
     5641 agactacaat aacaccttaa atcaacaaca agatgcaata aacaagacac tagacaacac
     5701 cttaaataat gtaaaaagaa ctactgatga agctacacga gagattccac gatacacaca
     5761 aaggattgct gaatatcaag aacaaactat tcaaactgta aaagatattg cttctgactt
     5821 tattgaagca gaaaaagaa
//
LOCUS       DASVEV010000296         7198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_161911, whole genome shotgun sequence.
ACCESSION   DASVEV010000296 DASVEV010000000
VERSION     DASVEV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161911"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..840)
                     /locus_tag="VF222_12890"
     CDS             complement(535..840)
                     /locus_tag="VF222_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143364.1"
                     /translation="MKHYKSTISFSIGFISLIIVTNNIGNANSQTYQEDPELSDLYLK
                     LSDLIKNTGDDVVQSMNAIESGDNKSALNILSSVTVNLQEISNGLNVMVNEPITRGN"
     gene            complement(1305..2015)
                     /gene="bioD"
                     /locus_tag="VF222_12895"
     CDS             complement(1305..2015)
                     /gene="bioD"
                     /locus_tag="VF222_12895"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX7143365.1"
                     /translation="MKSKGILIIGIDTEIGKTTIAAGLSHLLFRKGIEIGVMKPFATG
                     IKQYSKDYKSLDTKMLKDASGNKDNDNIINPFFYSIPTAPYLAKKILKLNENIDIEEI
                     LNKYKEIENRHDFTIVEGIGGLMVPLSKDFSVADLASILNIPIILITSNRIGSINHII
                     MTYRLAIFYKLKIKGIIINNKFKYLETYYKLITNSLPRVVEELTQCKVLATIPYIKKP
                     TAKKIANILEKYDFIKNL"
     gene            complement(2221..2904)
                     /locus_tag="VF222_12900"
     CDS             complement(2221..2904)
                     /locus_tag="VF222_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143366.1"
                     /translation="MKNELYSVLNEFGKEKIQIEFQNNINSSIPKIKEILSKTIATIY
                     EKKDNIDSDSNNFEKPLSILCESFLHFLLTTTSLPSQRKIMLNELEIDLVIPDIKTLY
                     KDPKKTLIIIFYKDSSTSEYIKNLDKIQKNKENIWLVSSTPLDINYRNYVLYENKEEY
                     QTLFTNNKTIDENFNNYLIDDKNENNNNNHNNIHNLTLNIIPFFNIIVDINKFLKDTK
                     YRGFKFVTN"
     gene            2959..3378
                     /locus_tag="VF222_12905"
     CDS             2959..3378
                     /locus_tag="VF222_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143367.1"
                     /translation="MAKIAIILLLISLGLLIIYGLDVMVASQSQTDAVTGGGGQGFLP
                     LNESVRGSIFGGGAVALSIIGFALGRKESNKTIPILLFINGGLIILGLFILMAMSNFE
                     IDQNTIRTIGSTMLLGGILIGLGIAKIVIDKKKLNSK"
     gene            complement(3512..3874)
                     /locus_tag="VF222_12910"
     CDS             complement(3512..3874)
                     /locus_tag="VF222_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143368.1"
                     /translation="MSYDRFVDERLLTSRDALNKYQMKIKLQDIDENARDYSQRFGNR
                     VLVRKIILMTKHNSTQEIEEIELDIEELEKRIKRERLFSSSNRWVAQNEIKNGYIVAT
                     RHLDLLSDAIALDVIVFD"
     gene            complement(3902..4276)
                     /locus_tag="VF222_12915"
     CDS             complement(3902..4276)
                     /locus_tag="VF222_12915"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7143369.1"
                     /translation="MHTKENNHQKFIEYIETGRFVLPYCKKCRKTMWPPSDYCRLCLN
                     NLSLKDYRNKEGKILQFFLNKYNNNTNTIMIVVELDEVRLIGSLDQDNRNTGNIEGKK
                     VIIKKCGFIDQKIFYHFEIVNN"
     gene            complement(4325..5467)
                     /locus_tag="VF222_12920"
     CDS             complement(4325..5467)
                     /locus_tag="VF222_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143370.1"
                     /translation="MRKVSVVGGVTSEFRKGTDIDVAHIALESALKLLKDTGFPRNLL
                     DAVIVSSCSSDQYLSSIISELLGVTPKISHRIENLCNSGTNAIISAFSYISSGLCDSA
                     LIIGVEKSNTLGKIFSNDLSRGQYNLPIFWGAIFKKIHMNRYGSTEEQICQIPVNNYL
                     KAKKNKNAINRSKSVNLEQVLNSRVLVQPLKLLECCSICEGSSSILLIADEKFSFFKS
                     KITSNKRNCELIPVLIKGIGQQTNCASFSNAIIDIFAQGSARMAAQQAYKMARISPQD
                     VDIAEIHDAFSILEILGYEDLGFVNKGEGGRFVDQNKIDINLRGGIIGCGHPIGVTGI
                     AQAVEIFEQLVGKNSHNLKLNKSPNVGVVHNLAAAGTSGTVLIMES"
     gene            complement(5468..6463)
                     /locus_tag="VF222_12925"
     CDS             complement(5468..6463)
                     /locus_tag="VF222_12925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7143371.1"
                     /translation="MKDISKIGYNIGTLLSIDAILRFAKKIEEKENTDSLWVPESWGR
                     EAFGSLGALSQVTKRVRLGTAIISIHSRTPATVAMGISTLDILSTNRSVLGLGVSTPA
                     LVENWHGVRFDHPIEKLSEYIKCIKMVMKGSKVIFDGKYYKINNFKLLFTPPRDNPPV
                     YTAAINKKMLDLSCELANGVLLYLRPKHELPKAVEYIKSKLKCKSNKKFEVCCAFITA
                     VSDKEPELARLRAAKTLAFYVAIGKYYNRFLRENGFETEVENITEEYQKNGLANVHNF
                     ISDKMLTSLTISGTREECIKSLREFNESGISLPIIQINPVGNNPENSIRELISTF"
     gene            complement(6460..>7198)
                     /locus_tag="VF222_12930"
     CDS             complement(6460..>7198)
                     /locus_tag="VF222_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7143372.1"
                     /translation="IFHSLIFNNLILDSILMKAVILAGGLGKRLKPLTNDRPKPMIEV
                     LNVPIIEWQLKWFKKHDIKEIIICVGYLKENIIDYLGSGNKLGINIGYIVEDNPLGTG
                     GALKNTSSLLNSSIDEGFFMTNGDILTNINPHKLSGEKISSIAVVPLRSPYGVVNING
                     ESNVIGFDEKPEIPDKWINAGVYYFTKDVFEYLPDNGNIEVTALPAMANDQKLKGMRF
                     NDNFWRSIDSYKDIDEAAKELQSSNLV"
BASE COUNT         2419 a         1086 c          936 g         2757 t
ORIGIN      
        1 atttaatgaa tcgtctagtt cgaaagtaat ttttaccata ttaccatata tacgtattac
       61 cctatttatg gtttcctgtt ttaccattta accggtttcc cattatacca tataaaaaat
      121 tgagattttg gacaaaacaa tataataaat tgaaattggt aaatcatagt tgtaaaatac
      181 tatactagga tagtaagaac aaatactagg caaatcaagc attaaaaaaa aagaaaactt
      241 atcgaaaaat tgagtttgag gaaaatcgag aatatcaaag ttcacgtcca ttaacaataa
      301 gaaagattat acatccataa tctcactgaa aataacaaaa aaacctgatc tatatccctc
      361 caataaaaag ttaattgtta taaagcatac gatttttctc agttaatatt gacgtcagaa
      421 aaaattgttg atattgaagt aattggaata aaattaataa tagaaaatta aaaaatatta
      481 ttaaaactgt tatatattgt aagtaaaata aagttatcgt tagtctataa aacgttaatt
      541 tccacgagtt atgggctcat ttaccatgac atttaatccg tttgatatct cctgaagatt
      601 tacagtgaca ctagatagga tattcaaagc agacttatta tcccccgatt cgatcgcgtt
      661 catggattgg acaacgtcat cgcctgtatt tttgatcaaa tctgatagtt tcagatataa
      721 atcagataat tctggatctt cctggtaagt ctgggagtta gcatttccta tattatttgt
      781 tacaataata agagatatga aacctatgga aaatgatatt gtggacttgt aatgcttcat
      841 gtctttcaat taaatttagt aattatataa atttttcaaa taaaaaacaa cacgctatac
      901 acactatatc taaagataaa aaaattaact gtaactagtt tgaatgttat tttttctttg
      961 atttgttgaa tacctatttt ttaacttaat ttaataattc aacaattctg gtcagtcatt
     1021 tattgtaatt tgaattttaa atatcactag atatgattaa catttcgtga ttaagttgac
     1081 tacttccata gcaaaatatg ctcttaacat tatgacaata ttctttaatt aattacatat
     1141 aatagagaaa atcaaggaca aatattttaa taacaaaatt gaaatgttta atatgataaa
     1201 caaaaaaacg ttaataaaga aattaatgtt ttaccagaaa aaacaattaa ttactcaatt
     1261 aaaatcgtaa gagaatgatc atatttcaat ttgtattata taaattataa attcttgata
     1321 aaatcatatt tttccaaaat attagcaatt ttttttgcag ttggtttttt tatatatggt
     1381 attgttgcca atactttaca ttgtgtcaat tcttctacta cacgtggaag actattagta
     1441 attaatttat aataagtttc taaatattta aatttgttgt ttattattat tcctttgatt
     1501 tttaacttat agaatatagc taagcgatat gtcattatga tatgatttat agatccaatt
     1561 ctatttgacg ttatcagtat tattgggata tttagtattg atgctaaatc tgcaacagaa
     1621 aaatctttgc tcaagggaac cattaatcct cctattcctt ctactattgt aaaatcatgt
     1681 ctattttcaa tttctttgta tttattcaaa atttcttcaa tatctatatt ttcatttaat
     1741 tttaatattt ttttggcaag gtaaggagcg gtaggtattg agtaaaaaaa gggattaatt
     1801 atattatcat tgtctttatt accgcttgca tcttttaaca ttttagtgtc aagagattta
     1861 taatctttag aatattgttt aattcctgtt gcaaaaggtt tcatgactcc aatctcaatt
     1921 ccttttcgaa aaagcaaatg gcttaaacct gctgcaatag tggtttttcc tatttcagta
     1981 tcgattccta ttattaagat tcctttactt ttcatagatt ttatttgtta tatcaatcat
     2041 aattacttac tcataataag aatgcaaaat ttgttagatt tttttggatc atcaattaga
     2101 gtagtatatt aaaaatagtt aagttctttt attgttcttg tttagagtct ttatagaaac
     2161 gtatttaaag aaaaataaaa gaagtaataa acaaattagg ttataattat ttaatatgta
     2221 ttaattagtt acaaatttga aacctctata ttttgtatct tttaagaatt tattaatatc
     2281 gactattata ttaaaaaaag gaataatatt tagtgtaagg ttatgtatat tattatgatt
     2341 attattatta ttttcatttt tatcatcaat aagataattg ttaaaatttt catctatagt
     2401 tttattgttg gtaaataaag tttgatactc ttctttgttt tcatatagaa cataatttct
     2461 ataattaatg tcaagaggag tagatgaaac tagccaaata ttttctttat tcttctgaat
     2521 cttatctaaa ttctttatat attcactggt agatgaatct ttataaaata ttattatcaa
     2581 agttttcttt ggatctttat atagagtttt tatgtcagga attaccaaat caatttctag
     2641 ttcatttagc attattttcc tctgcgaagg aagtgaggtt gttgtcaata aaaaatgtaa
     2701 aaaagattcg cataatatag acaatggttt ttcaaaatta ttggaatcgg aatcaatgtt
     2761 atcttttttt tcataaattg ttgctattgt tttagatagt atttccttta ttttgggaat
     2821 tgatgaattt atattatttt gaaattctat ttgaattttt tcctttccaa actcatttaa
     2881 gacagaatac aactcatttt tcatatccac attattaatt attgataggt ttattaaata
     2941 tcttaatgta aaaaaatcat ggcaaagatt gctattatcc ttttattaat ttcgcttgga
     3001 ttgctaataa tttacggttt agatgtaatg gttgcatcac aatctcaaac agatgcagtt
     3061 acaggaggag gaggacaagg tttccttccc ctaaatgaat ctgtaagagg aagtattttt
     3121 ggaggcggag ccgtcgcatt atctataata ggatttgccc ttggtcgaaa agaatcaaac
     3181 aagacaatac ctatacttct atttattaat ggaggattga tcatcttggg attatttatt
     3241 ctaatggcta tgtctaattt cgaaatagat caaaatacta tacgaactat aggttcaaca
     3301 atgctattgg gaggaatatt aatcgggtta ggaatagcca aaattgtaat agacaagaaa
     3361 aaattaaact cgaagtaaat tttttctttg gtcgtttatt ttactaatta aatattattt
     3421 tcattcataa tataatatgg ttgcaactaa taccaaacaa aaaaacaata aagtgcatct
     3481 gttatttttt aattcttgaa aaattaaatt tctaatcaaa aactattaca tctaacgcta
     3541 ttgcatctga caaaaggtct aggtgtcgtg ttgcaacaat ataaccattt ttaatttcat
     3601 tttgggctac ccatcggttg gatgaggaaa aaaggcgttc tctttttatt cttttttcca
     3661 attcttcaat atcaagttca atttcttcaa tctcttgagt actgttatgt tttgtcatta
     3721 agattatttt tctaactaaa actctatttc caaatctttg agaataatca cgcgcatttt
     3781 cgtctatgtc ctgtaacttg attttcattt gatatttatt taaagcatct cgactagtca
     3841 acagtctttc atcaacaaaa cgatcgtaac tcaataaaaa taaaataagc aaatagatat
     3901 attaattatt cacaatctcg aagtgataaa atattttttg atcaataaat ccacattttt
     3961 ttattattac tttctttcct tcaatattac ctgtatttct attgtcttga tcgagagatc
     4021 caataagtct tacttcatct aattctacta ctatcattat tgtatttgta ttattattat
     4081 atttattaag aaaaaattgt aatatttttc cttctttatt tctatagtcc tttaatgaaa
     4141 gattattcag acataatcta caatagtctg aaggaggcca catagtcttt ctacatttct
     4201 tacaataagg aagcacaaac cttcccgttt ctatatattc gatgaatttt tgatgattat
     4261 tttcttttgt gtgcatggtt gtttttgttt aggtctaagg aaaatgttag aatatgctca
     4321 aaatctaact ctccataatg aggacagtcc cagaagtacc tgcagctgct aagttatgga
     4381 cgacacctac attaggagat ttgtttaatt ttaaattatg actgtttttc cctactagtt
     4441 gttcaaagat ttctacagct tgtgcaatcc cagtgacccc tattggatga ccacatccaa
     4501 ttattccacc ccttaagttt atatcaatct tgttttgatc aacaaatctt cctccctctc
     4561 ctttattgac aaaaccaaga tcttcatacc caagtatttc caagatagag aaagcgtcat
     4621 gaatttctgc tatatccacg tcttgagggg aaatcctagc cattttatat gcttgttgtg
     4681 ctgccattct agctgaacct tgggcaaaaa tatctataat tgcattacta aaacttgcac
     4741 aattggtttg ttgaccaatt cctttgatta aaactggaat cagttcgcaa tttcttttgt
     4801 tagatgttat tttagatttg aaaaatgaaa atttttcatc tgcaataagc aaaattgaag
     4861 aactaccttc acaaatagaa cagcactcca ataatttaag aggttgaaca agtactcttg
     4921 aatttaaaac ctgttcaaga tttacagatt tagacctatt aatggcattt ttatttttct
     4981 ttgctttaag gtaattatta acaggaattt gacagatttg ctcttctgtt gatccatatc
     5041 tattcatatg aattttctta aatattgcac cccaaaatat aggcagattg tattggcccc
     5101 ttgagagatc atttgaaaaa atttttccta atgtattaga tttttcaact ccaataatta
     5161 aagcagaatc acataatcct gatgagatat aagaaaatgc ggaaattatt gcattggtgc
     5221 cagaattaca taggttctca atcctatggg aaatctttgg agttactccg agcaactccg
     5281 aaattatgct agataaatat tgatccgaag aacatgatga tactattact gcgtcaagca
     5341 agtttcttgg aaatcctgta tctttcaata acttcaatgc tgattctaag gcaatatggg
     5401 caacatcgat atctgttcct tttctaaatt cacttgtgac ccctcctact acagaaactt
     5461 ttctcaacta aaatgtagag attaattccc taattgaatt ttctggatta tttccaacag
     5521 gatttatttg gattattggt aaagatattc cactttcgtt aaattccctt aaagacttta
     5581 tacattcctc tcttgtacct gatattgtca aagaagtaag cattttatct gaaataaaat
     5641 tatgaacatt tgcaagtcca ttcttttgat attcttcagt aatattctct acttctgttt
     5701 caaaaccatt ttctcgtaaa aatctattat aatactttcc tattgccaca tagaaggcta
     5761 gagtctttgc tgctcttaat ctagccaact cgggctcctt atcggaaact gcagtgataa
     5821 atgcacaaca aacctcaaat tttttattac ttttacattt taatttgctt tttatatatt
     5881 caacagcttt tggtaattcg tgtttaggtc ttaaataaag caaaacacca ttagctaatt
     5941 cacatgaaag atctaacatt tttttattaa ttgctgcagt atatactgga ggattatctc
     6001 taggtggtgt gaataataat ttaaagttat taatcttata atattttcca tcaaaaatta
     6061 cctttgatcc cttcataacc attttaatac acttgatgta ttcgcttagt ttttcaattg
     6121 gatgatcaaa tcttactcca tgccaatttt ccaccaatgc aggagtactc actcctaatc
     6181 ctaatactga tctgttagtg gataatatat ctaatgttga tattcccatc gccactgtag
     6241 caggagttct ggaatgtata cttattattg ctgtaccgag tctaactctt tttgttacct
     6301 gtgataaagc acctaatgat ccaaaggctt ctctccccca tgattctgga acccataatg
     6361 aatctgtatt ttctttttct tctatttttt ttgcaaatct taaaatggca tcaattgaca
     6421 atagtgtacc gatattatat cctattttgg aaatatcctt catactaaat tagatgattg
     6481 aagttccttt gcagcttcat ctatatcttt ataagaatct attgatctcc aaaagttatc
     6541 gttgaatctc atgcctttta atttttgatc attagccata gcaggtagtg cagttacttc
     6601 tatgtttcca ttatcaggta aatattcaaa tacatctttt gtaaaataat aaacgcctgc
     6661 atttatccat ttatcaggaa tttcaggttt ttcatcaaat cctattacat tagattcacc
     6721 atttatatta actactccat atggactccg aagaggaaca actgcaatac ttgagatttt
     6781 ttctcctgat aatttatgtg gattaatatt tgttaatata tcaccatttg tcataaaaaa
     6841 tccctcatcg atggatgaat tcaacagact ggatgtattt tttagagctc caccagtacc
     6901 aagaggatta tcttctacta tatatcctat attaataccc aatttattac cactaccaag
     6961 ataatcgatt atattttctt taagataacc aacacatata attatctctt taatatcatg
     7021 ttttttaaac cattttagtt gccattctat tatgggtaca tttagtacct ctatcatggg
     7081 tttgggtcga tcatttgtta aaggttttaa tcttttacct aacccaccgg caagtataac
     7141 agctttcatc aaaatagaat caagaatcag attattaaag attaatgaat gaaatata
//
LOCUS       DASVEV010000297         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_162756, whole genome shotgun sequence.
ACCESSION   DASVEV010000297 DASVEV010000000
VERSION     DASVEV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162756"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF222_12935"
     CDS             <1..427
                     /locus_tag="VF222_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143373.1"
                     /translation="DEKYDPQTKTCIPNCSLDEKYDPQTKTCIPIQDIDVKIRITTEN
                     SGSHDYKAGGDIDLVLGKKEKLVQIEFLLPDQCKTITSNILSHDPDLHPFLKKPIHGD
                     LSKDSLDENKFIYSLKPGNIEEALNYELDLEYCLNSNER"
     gene            495..2138
                     /locus_tag="VF222_12940"
     CDS             495..2138
                     /locus_tag="VF222_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7143374.1"
                     /translation="MKIVFGLVLLLNLIISNLILSEGQMKIEGKITIKTNEDNQPSGE
                     PGSPNQSIKPEIFETITVGDLNITDTEKIKTDVGGQPIPFQYIVIFDDKFTANPNSLA
                     TTFQALSLIVESLGAEVIFTYHNTISGFAFKTIDQQKSDQILDILSLDPRVKFAEQDK
                     IVVPFSQVIPSGIQRIGIENVAMGSDIEHSSIDADIAIIDSGIDLDHPDLNVFKNVTS
                     IIPQILSSKDNTIRAKDIDLNKIGLNVENNLTSFYPPFSQDSSSTGDDNCGHGTHVAG
                     IAAAKDNAFGVIGIAPGAKLWAIKVLDYDKNSGKCFGSISSVIAAIDYVTKNADQIDV
                     ANLSLGCKCNSTALDEAISKSVVENVVYVTAAGNINTDASFFSPANHREVLTVSAIAD
                     ADGKCGSLGTPLWVDAGDFSGFSKDDTFASFSNYGSVVDIAAPGVKINSTYINGTYAL
                     MGGTSIASPHVTGMVALYKSINPTASPKEISEKLTNGGLYPNTVCDGYSYGYFDQDLD
                     NTPEPLLHSFDMVNIKDDESNIKSDPDFWNTFSDIAELR"
     gene            2318..3106
                     /locus_tag="VF222_12945"
     CDS             2318..3106
                     /locus_tag="VF222_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143375.1"
                     /translation="MNYKNLHFFCFNYANFDYTTSIIIIILFNLSLLQISFSIEQINN
                     NQIGNVTEKEIKKVVDQDILYSKSVNNKTNIVVILPSTNNDTIYNGIITYSSNQPVYI
                     GIQHNINNNSSKVNSSAIIENKKFFTSMIYPDYIVKEKIYSSSLPFVGSALTFNYEKP
                     FFVIYSIIATIEKYEKNTNSQNSLISDTQSIKNQQIDPFGPPTAYPTNGLSSTKLLKE
                     VLPYLSIEILSQLPLDQLPNKECNEILEKLSIEERNKITCKKEY"
     gene            3316..4434
                     /locus_tag="VF222_12950"
     CDS             3316..4434
                     /locus_tag="VF222_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143376.1"
                     /translation="MPILSIIIYANIFNLDVYAYDNEISPSFDRQVIKDPPLDYIIIN
                     QQNQNIVKSEEKLPLADIVEVSYFSNGKTLKSTFWLLLPIMPSQYKINYGLFIDSDNN
                     DQTGFNGIDYQYEISWINETKSWNKRVLEWASSGEERILDEIVNLTDFSKGQNYISLS
                     LDLKKLLYPKEYRVLFYAEYEKNNLLISDFSKWVYVPPPNIEINTNPQNIVLRQGEQK
                     NIELNLNSTFGLEPLVNVSFINRYKDLEIESEYNSIQLPYYGEISIPVKIKALKNASI
                     SPHTIFLKAVSTFPDIEFIKKNESSGSNESLPINIESQEEEKRLKLVISVGRSFSISE
                     RIGILWEQIGGPFIFFYGVIAGLSPWIIREVKRRINNK"
BASE COUNT         1748 a          621 c          701 g         1553 t
ORIGIN      
        1 agatgagaag tatgatccac agacaaaaac ctgcatacct aattgttcat tagatgagaa
       61 gtatgatcca cagacaaaaa cctgcatccc catacaggat atagatgtaa aaataagaat
      121 aaccactgaa aatagtggat ctcatgatta taaggctggt ggagatatag atcttgtact
      181 aggtaagaag gaaaaattag ttcaaattga atttctacta cctgatcaat gtaaaactat
      241 tactagtaac atattaagtc acgatcccga tcttcaccct tttcttaaaa aacctataca
      301 tggagattta tccaaagatt cattagatga aaataaattt atatatagtc taaaaccagg
      361 taacatagaa gaagcattaa attatgaatt agacttggag tactgtctaa acagtaatga
      421 aagatgatga aaaataataa cattcaattt atgataataa tcaaagtgga gtatatcttg
      481 agttgaaaat cattatgaaa attgtatttg gtttagtatt attactaaac cttataatta
      541 gtaacttaat actttcagaa ggacagatga aaatcgaagg gaagattaca ataaaaacta
      601 atgaagacaa tcaaccatct ggtgaaccag gatctccaaa tcagtctatt aagcctgaga
      661 tttttgagac tatcacagta ggcgatttaa atattactga tactgaaaaa attaaaaccg
      721 atgtaggagg tcaaccaata ccattccaat atatagttat ttttgatgat aaatttacag
      781 caaatcctaa tagtctagct actacattcc aagccttatc acttatcgta gaaagtttag
      841 gtgcagaagt tatctttact taccacaata cgatatccgg ttttgctttc aagacaatag
      901 atcaacaaaa aagcgatcaa atattagata ttcttagttt agatccacgc gtaaaatttg
      961 cagaacaaga taaaattgtg gttcctttct cccaagtaat tcctagtgga attcaaagga
     1021 taggtataga aaatgtagcg atgggttctg atatagagca tagtagtatt gatgcagata
     1081 ttgctattat agatagtggt attgatttgg atcatccaga tctaaatgtg tttaagaatg
     1141 ttacctctat tataccacaa attttatcat ctaaagataa tactataaga gcaaaagaca
     1201 tagacttaaa caaaattggt ctaaatgtag aaaataacct gacttctttt taccctcctt
     1261 tctcacaaga ctcaagttct acaggagatg ataattgtgg tcatggaacg catgttgctg
     1321 gtattgctgc tgctaaagac aatgcttttg gagtgatagg catagctcct ggtgcaaaat
     1381 tatgggctat caaggtttta gattatgata aaaattctgg taaatgtttt ggatcaattt
     1441 cctccgtaat tgctgcaatt gattatgtaa caaaaaatgc agatcaaata gatgttgcaa
     1501 atttaagtct tgggtgcaaa tgtaattcta ctgcattgga tgaagcaata agtaaatcag
     1561 tagttgaaaa tgtagtttac gtgactgctg ctggaaatat caatacagat gcatcatttt
     1621 tttcacctgc caatcataga gaagttttaa cagtatcagc tattgcagat gctgatggga
     1681 aatgtggatc tttgggaacc cctctatggg ttgatgcagg tgatttttca ggtttttcta
     1741 aggatgatac atttgcatct tttagtaatt atggatcagt tgtagatatt gcagcaccag
     1801 gtgtaaaaat taattcaact tatataaatg gaacatatgc attaatggga ggtactagca
     1861 tagcatcacc acatgtaaca ggaatggtcg ctttatacaa gtcaataaat ccaactgcat
     1921 cgcctaaaga aattagtgaa aaattaacga atggtggatt gtatcctaat acggtttgtg
     1981 atgggtatag ttatggttat tttgatcaag atttagataa tactcctgaa cctttgctac
     2041 attcatttga tatggtaaat atcaaagatg atgagtccaa catcaagtcg gatccagatt
     2101 tttggaatac cttttccgat attgctgagt taagataaat attgctcatt tgaaatcaaa
     2161 ttttgatttg tctattgatt aaaatttgat aaaaattttg aagaatattt tcttaacaaa
     2221 ttaactttat gaagttttta aatactattt aataattatc tctaaagtca atatatatta
     2281 aaattactta tataaaagaa catattttat attaattatg aactataaaa atttacattt
     2341 tttttgtttc aattatgcga actttgatta tacaacttct attataataa taatattatt
     2401 taatctatct ttattacaaa tatcattctc aattgaacaa atcaacaata accaaatagg
     2461 aaatgtaact gaaaaggaga ttaaaaaagt agtagatcaa gatatattat attctaaatc
     2521 ggtaaataat aaaactaaca tcgttgttat tcttccttca acaaataatg acacaatcta
     2581 taatggtata ataacatata gttcaaatca acctgtatat attggcattc aacataatat
     2641 taataataat agctcaaaag ttaattcaag tgctatcatt gaaaacaaga aattttttac
     2701 ttccatgatt tatcctgatt atatcgtgaa agagaaaata tattcctctt cactaccatt
     2761 cgttggaagt gctttaacct ttaattatga aaaacctttc tttgtaattt attccataat
     2821 tgctacaatt gagaaatatg aaaagaatac aaattctcaa aattcactaa tttcagatac
     2881 tcaatcaatc aaaaatcaac agatagaccc ttttggtcct ccaactgctt atcctactaa
     2941 tggtttaagc tctactaaat tattaaaaga ggttttacct tatttatcta tagagatact
     3001 aagccaacta cctctggacc aacttccaaa taaagaatgc aatgaaatct tggaaaaact
     3061 ttctatagag gagcgtaata agattacctg taagaaagaa tattaagtta caacccgagg
     3121 taaaataaat agataaaata attaaataaa ttagtataaa actagtactc aatatcctga
     3181 gaatgtcttt taataatgga aattaatttt aaagcctaaa aaatgttaaa ttatctatta
     3241 taagaaaata tttatttatc taaccaatat atttagttac tataataaaa aatgaattta
     3301 ctaagatatt tcgtattgcc tatcttatct atcattattt atgctaatat atttaattta
     3361 gacgtatatg cctatgataa tgaaatttct ccatcctttg atcgtcaagt tatcaaagat
     3421 ccaccattag attatataat tattaatcaa caaaatcaaa atattgttaa atcagaagaa
     3481 aaattaccat tagctgatat cgttgaagta agttatttta gtaatgggaa aacattaaaa
     3541 tctacttttt ggttactttt acctattatg ccatcacaat ataaaatcaa ttatggatta
     3601 tttattgatt ctgataataa tgatcagact gggtttaatg ggattgatta tcaatatgag
     3661 attagttgga taaatgaaac taaatcatgg aataaaagag tattagaatg ggcttccagc
     3721 ggagaagaaa gaattttaga cgaaattgtt aatttaacag attttagtaa aggtcaaaat
     3781 tatatttcat tatcattaga tcttaaaaaa cttctatatc ctaaagaata tcgagtttta
     3841 ttttatgcag aatatgaaaa aaataatttg ttaatttcag attttagtaa atgggtttat
     3901 gttcccccac caaatataga aattaataca aaccctcaaa atattgtatt aagacagggc
     3961 gaacagaaaa atatagaatt aaatctgaat tctacttttg gattagaacc tttggtaaat
     4021 gttagtttta ttaataggta taaagattta gaaattgagt cagaatacaa ttcgattcag
     4081 ttgccatatt atggagagat atcgattcct gtaaaaataa aagcattgaa aaatgcttca
     4141 atatctcctc atacaatatt tcttaaagca gtttctactt ttcctgatat cgaattcatt
     4201 aagaaaaatg aaagtagtgg tagtaatgaa tctctaccta ttaatataga aagtcaagag
     4261 gaagaaaaaa ggctgaaact cgtaattagc gtaggcaggt ccttttctat atcagagaga
     4321 atcggcatat tatgggagca gataggtggt ccttttatct ttttttatgg agtaattgca
     4381 ggattatcac cttggattat tagagaagtc aagaggagaa taaacaataa ataattttat
     4441 tggcagataa aaggggtgta ataagattaa ttgcttattt tccctaatcg aattattgaa
     4501 ctaatacata ctattgtatt agtattgaat atagggtcat gtgttgggta ttgacaaagg
     4561 tttcagttat ccatctacca attagaattt aattaaattg ataacatgat ttctttatta
     4621 aat
//
LOCUS       DASVEV010000298         5273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_164608, whole genome shotgun sequence.
ACCESSION   DASVEV010000298 DASVEV010000000
VERSION     DASVEV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164608"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            826..1482
                     /gene="hsp20"
                     /locus_tag="VF222_12955"
     CDS             826..1482
                     /gene="hsp20"
                     /locus_tag="VF222_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HEX7143377.1"
                     /translation="MSFPNDIDPYNFFRNLFGGSGGRRRREGRGFGGWSFDDMFREFD
                     DMRRETERIFSEQFKNIENKVPKDLIKEYDTPEGGKVREVGPIVYGYSMTIGPDGKPK
                     IQEFGNLKSFSGRGSFEQPSLSAEREPLIDISTTDKEVKIVAEMPGISKEKIKIDAYD
                     KYVEIKSEDPNRKYHKKIEVPEDIDIESARSNYNNGVLEITFQKREQQKPKGRQINIE
                     "
     gene            complement(1562..2236)
                     /locus_tag="VF222_12960"
     CDS             complement(1562..2236)
                     /locus_tag="VF222_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7143378.1"
                     /translation="MDYSNEKNGSIDDGVINTNSSNNKDISSSERDIIKLKQTKAKFV
                     KRIFISGIIFTIAFVTIQYFLKPIISKDYLMLLQSIQVSIVGYFVIEIISNAFHDLTY
                     DYIKDTSKSIKILMRIVGAIVVIAIIISYLSNDPVVAAAIGTISGLVVGFASQNIIGN
                     IIAGLYLALLRPFKLGDEITAFNNTGVIYDIGLISTKLKTTDNKTVLVPNSSMVTTTI
                     VLNKRQ"
     gene            2778..3350
                     /locus_tag="VF222_12965"
     CDS             2778..3350
                     /locus_tag="VF222_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143379.1"
                     /translation="MKYQNIFVYAILAIGILSIPSLLESALAQTNSTNQTATQNQTAT
                     QNQTATQNQTSTQNQTMSKDTKALMTMDLPGLKDTIANAKQSLANGDTEEALTQITDV
                     ENKVLLLKPQPSFASDIQKIKDSISKKDVNKALDDLTKAQTNLLKAETEMLKAQVANP
                     MLAQQNNEQNQGDGDGGGDGDGDGGGDDTN"
     gene            complement(4473..>5273)
                     /locus_tag="VF222_12970"
     CDS             complement(4473..>5273)
                     /locus_tag="VF222_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143380.1"
                     /translation="AYAQKLLKYWVHLLNYKSFNNLNTEKIKKSKRGIVFSQYMKDKI
                     GGGDIIYHGSEPMIPYDSTIKGKARTKFSLSHKKRIILALGFRTNTKGWDILDEMDIP
                     PNWAIVMNSSKNYYNTENYIPKISANHEKEIIDLHKGFLNDRDLSLLFYAADAVILPY
                     KVSSGSGVMFDGLAHGLPFIATDLEFFREFSAKGLGITVKRNPQEFSKALVTLNNNYE
                     YYAKNVILFKEKLKWYNIARNHISLYNQIIEQNSVAPTIKRTNSNNLH"
BASE COUNT         1954 a          740 c          740 g         1839 t
ORIGIN      
        1 aaaatgcaat gtaatactaa gagattttaa aatataaagg aattcaagaa tcagcatatc
       61 aaaaaggatt agagttatat aataaaggct tggattcgag taaggttaac tattctcaaa
      121 agactaaatg gattgttgtc gaaaataaca tttctattat attaattatc caatatatta
      181 gtaaaataat tcaattcaga taaggatata gtgttattaa gattgttata aattttaaag
      241 caatagattt ccgatatttt tacttcgagt ttgtcacata ttattactta aattttgatg
      301 atgagatcca ttgaagcttt gcatcaaaaa gaatagtgtg aatcagattg atctagttac
      361 gattattacc ggtagcatat ttgagtaact atcaatacca tattgatatt atttatgcta
      421 tgtataaaga tttcatgtta tggcaacacc atcacgactg ataattttac ataagcttac
      481 tggttgtact atttattaaa ttaaacagta taagatataa atccataata tgtagatttc
      541 aatactttaa tagctcctct accttaaaat agagtatttg gagttaattg acttccagca
      601 aaattgttaa ttagaaaagc attagagaga tttaacttaa tagaaaaaac atgcaatatt
      661 aaaatgaaca tctattacca ccaagtgtta tagacacgta aactcgtcac tagcacaaag
      721 aaaatctttt ttttatttct tatttttctt tgcttcaaat tcattagtgg gtaatcgaaa
      781 attcattttg tataacttta ttacaaagta atgtattatc attatatgag ttttccaaat
      841 gacatagatc catataattt ctttagaaat ttgtttggtg gtagtggagg acgtagaaga
      901 cgtgaaggaa gaggttttgg gggatggagc tttgacgata tgtttagaga atttgacgac
      961 atgagaagag aaacagaaag aatattttct gagcaattta aaaatattga aaataaagta
     1021 cctaaagatt taattaaaga atacgataca cctgaaggcg gaaaagttag agaggttggt
     1081 cctatagtat atggatactc catgaccata ggtcctgatg gaaaaccgaa aatacaagaa
     1141 tttggtaatc taaagtcttt ttcggggaga ggatcctttg aacaaccatc actttcagcg
     1201 gaaagggaac cgttaataga tatctcaacg acagataaag aagtaaaaat tgtagcagag
     1261 atgcctggaa taagtaaaga aaaaataaaa atagatgcat atgataaata tgtagaaata
     1321 aaaagtgaag atcctaatag aaaatatcac aaaaaaatag aagtaccaga agatattgat
     1381 atagagagtg caagatcaaa ttataacaat ggagttcttg aaataacatt ccaaaagaga
     1441 gaacaacaaa aaccaaaagg tagacaaata aatatagaat aaccaattga attgatacaa
     1501 tactaagctt atttttatga ttttcacatt attgaataaa tgaaaatgta taatcacatc
     1561 tctactgtct tttatttaat acaatagtgg tagtaaccat agaagaatta ggaactagaa
     1621 cagttttatt gtcggtagtt tttagtttgg ttgaaattaa gccaatgtcg tatattactc
     1681 ctgtattatt aaatgcagta atctcatctc ctaatttaaa tggcctaagt aaagccaaat
     1741 ataaccctgc aatgatattt ccgattatat tttgggaagc aaaacctact accaaaccag
     1801 atatagttcc tattgctgca gctacgactg gatcattact taaataggaa attataattg
     1861 ctattactac tattgcacct acaatcctca tcagaatttt tatagatttt gaagtatctt
     1921 ttatataatc ataggtaaga tcatgaaatg cattactaat tatttctatt acaaaatagc
     1981 ctactattga gacttgtatg ctttgtaata gcataagata atcttttgat attataggct
     2041 ttagaaaata ctgtattgta acaaatgcta ttgtaaatat aatacctgaa ataaaaattc
     2101 ttttaacaaa tttcgcctta gtttgtttta atttaataat gtctctttca gaggaactga
     2161 tatctttatt attactacta ttagtattaa tgacaccatc atcaatacta ccattttttt
     2221 cgttagaata gtccatacat ccataactta atgtactgaa ataagtttat tgataatatc
     2281 aagttattat tatcataatt aacaaatgaa agtaatgtta actttttata tttttggcaa
     2341 atatgatgtt ttgtcaatag tagaagatct ttctcaccga atagcaaatg tcttgttttt
     2401 cttaaaaact ctatcttaat acaaacccat aatattaaag tcatttaaat taattgaatc
     2461 tgttttaata aattaagaag atgcatgcta tgtatatctt ttctaaattt ctttaatact
     2521 ttgaaaacct ttctctactc cagtattgag cccaattgac ctaaagatcg actaaaacta
     2581 tagatttcat tattttctac tttttcaata attactacta ctacaatctt taatttatca
     2641 caatccacaa gtatgagatc aagtataata tgctagtaat attgtaataa tttctaaatt
     2701 aattttaaat aaagacattt agtctgattt tcattaaatt aaagcattat aagtcatatt
     2761 gtacaaagtt atttagaatg aaatatcaaa atatatttgt ctatgcaata cttgcaattg
     2821 gaatattatc aattccatct ttgttggaga gcgcactagc tcaaaccaat tcaacaaacc
     2881 aaacagcaac acaaaaccaa acagcaacac aaaaccaaac agcaacacaa aaccaaacat
     2941 caacacaaaa ccaaactatg agtaaagata ctaaagcatt aatgacaatg gatcttcctg
     3001 gattaaagga tacaatagcg aatgcaaaac aatcattagc taacggagac accgaagaag
     3061 cattaacaca aattacagac gttgaaaata aagtgctact attaaaacct caaccttcat
     3121 ttgctagtga tatacaaaaa attaaagatt caatttccaa aaaagatgta aataaggcat
     3181 tagatgatct taccaaagct cagaccaacc tattaaaagc agaaactgaa atgttaaagg
     3241 cccaagtagc aaatccaatg cttgctcaac aaaataatga acagaaccaa ggtgatggcg
     3301 atggcggcgg agacggtgat ggcgatggcg gcggagacga caccaactaa ttttttttaa
     3361 atcgtaatac tttaattaat ttgccgactt tgatttttga aaaatttaat ccgttttaat
     3421 atactcgcta ttaatatttt tggtaataat taaggtatga atatttgcat aatcggcaaa
     3481 tatccattaa acttcattga ttctggagaa tatttgtaat cgcctaccat ttttattcaa
     3541 aatattaatt atttaaaaat catttttgct ctgaatatat agcagaggag aaaaaatctt
     3601 gaaaattaaa gaatgataaa tgtattaata tagttccttg ttaaatgcct tattcattga
     3661 atacataatt gatctttaat tagtcaaatt ataaataata tcccgcagaa gacctgctct
     3721 tgcaactact tatgctctgt gggaaatata tgagcgatcc gaagactact aataatcgat
     3781 aaattagaat tgatgcattg gttcctataa tattatattc taaaattctt tataattttt
     3841 gattcaatca aaaattttcc agccatcatt ttcctctatt actttaccaa ttcctatatc
     3901 tttataaaaa taaatagatt ttttatagta acttcttatt tttccatttg ctttcaactt
     3961 ttgcttaata aaccctcccc cagcaatttt tgatttgtaa aaattagatg tagtagagca
     4021 tgataatata ccgctatttt tcttttttta taccagatct tatatataca taaagattcc
     4081 aaaatagaat tattcttaat tgatattttt catatttagc tgtatttctt tcagtggtct
     4141 aaatgtattt gccagaggtc tttgatttaa tagggactac ttcaatacta aattgtagaa
     4201 agatattatt taagattatt aactatgatt tagattatta agatgaagtg tattaagtat
     4261 ctatgacaat taaagagcaa tattgggatc gatatttaaa tcgagaataa taatttacta
     4321 tctattcagt tatattatca agtaatatga tactaatctt gatgatacat atatgaatta
     4381 tataaaataa agaataattt gagactagca ctttttaagt agtttatcta caaataggtt
     4441 ggtggtggtg gtggtagcat tggaagtttt tatcaatgca aattattgga atttgttcgt
     4501 tttattgttg gtgctacact attttgttca attatttgat tatacaaact aatatgattg
     4561 cgcgcaatat tataccattt tagcttttct ttaaatagaa tcacattctt agcataatat
     4621 tcatagttat tgtttagagt caccagagct ttggaaaatt cttgaggatt tcttttcaca
     4681 gttataccta acccctttgc agaaaattct ctaaagaatt ccagatctgt agctatgaat
     4741 ggtaatccat gtgccaatcc atcaaacata acaccagatc ctgagcttac tttatagggt
     4801 aaaattactg cgtcagctgc ataaaacaaa agcgaaagat cacgatcatt taaaaaacct
     4861 ttatgtaaat caatgatttc cttttcgtga ttagcagata tctttggaat ataattttct
     4921 gtattataat aattctttga agaattcatt acgattgccc aattcggagg aatatccatt
     4981 tcgtctaata tgtcccatcc tttagtatta gttctgaatc ctaaggccaa aattattctt
     5041 tttttatgtg aaagagaaaa cttggttcgg gcttttcctt tgatagtaga atcataaggt
     5101 atcattggtt cagagccatg ataaattata tcacctccac ctattttgtc tttcatatat
     5161 tgtgaaaaaa ctattcctct tttacttttt tttatttttt ctgtatttaa attgttaaaa
     5221 gatttatagt ttaaaagatg tacccaatat ttcagaagtt tctgtgcata ggc
//
LOCUS       DASVEV010000299        17520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_748432, whole genome shotgun sequence.
ACCESSION   DASVEV010000299 DASVEV010000000
VERSION     DASVEV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 17520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17520
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748432"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF222_12975"
     CDS             <1..738
                     /locus_tag="VF222_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143381.1"
                     /translation="SKFGTPGSGTLAGLLTDNGILEEELTYDDVMGMIETKLESVSNT
                     TNTLAPSRTLGEELNSEVMNMIETKLESVSNTTNTLAPESIPIKDFYTKSILGDSLSI
                     DTDGGADDDKVVDSSSSVSETLSSITKEDNLISLFDESFNEYIVSPFNKYIVSSLNSS
                     SFIDKIKSHNYDIDYNETINNLESLKTTFDSSLGGLAEDDLITDPIDQRNVYSEIDSA
                     IDILKANSCLEDNCVVLEKHPNETLVY"
     gene            940..1776
                     /locus_tag="VF222_12980"
     CDS             940..1776
                     /locus_tag="VF222_12980"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX7143382.1"
                     /translation="MVKGLALTIGLNFVDPTHYAGWDGQLSACEYDAEDMTDIAKSKG
                     FEVQKLLTKTATSGNVENGISKAAKKLESGDIFLLSYSGHGGKLPDKNSDEVDQTDET
                     WCLYDRQLVDDELNNCLAQLTKGVRVLVFSDSCHSGTVTRAIAANKNIDQMNSNIDNQ
                     GKKYRFAPKNVIARTYKNNKTQYDKILTNRNLKESEDKVKASVILISGCQDNQYSQDG
                     EFNGLFTSNLLDVWKEGTFKGSYKRLHKSIIGQMPPDQTPNYYLVGTLDKKFEKQIPF
                     TI"
     gene            complement(1903..3432)
                     /locus_tag="VF222_12985"
     CDS             complement(1903..3432)
                     /locus_tag="VF222_12985"
                     /inference="COORDINATES: protein motif:HMM:NF015332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7143383.1"
                     /translation="MQSKYDQITILLNKLKEEKKDIKNYLKLLNEIIKLTIDLCNYFY
                     RNNDLNPILQFYREITLGYEKAMTIIIQDSELSELSSSIRDSYEYWKFNQQIIQKELM
                     KLNIQEGNMLLLEEKYEHASSKFKNALEYEKNNIDILNKLGVSYFHSHKFDDALRCFG
                     NAMQLDENNVLLLYNSALMYEMLWNTSDYLNNFYKTSAIILYERALTIKPQHYESLIS
                     LGLLFYKTEDYENAKLKLEDAMKIQNNDWRLLLAYGCVLSDGFYKYEEAKDIFNKSIA
                     LNPNSIVAKMNLSQVLILLNKYNESERHLKEILKKLEGIEDRSTSLILRILLICFQAL
                     SQEKISKIFVDELLEFCELKNLELVHWNFNNLISHVNNSKIDIKLRKLLSLILTIPKI
                     NESNKEQILQQIRKLANQQQIGSEKIKIASKSIVDKQHEPWYYWELCVKAPPEVMQSI
                     ISVEYVLDPTYKESKKTVFTQENGFLLKGKGWRNFNLLANILLDNKKVFKKYHKIILA
                     K"
     gene            complement(3477..4904)
                     /locus_tag="VF222_12990"
     CDS             complement(3477..4904)
                     /locus_tag="VF222_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7143384.1"
                     /translation="MSSKITVLFMAAAPDDKIYVNVVKEGTEIQQTLDGQRDKFQFFH
                     ILGITKDHFLDDLERYRPNILHFSGHGTESSSLVFQDGDELSSEQLEKTFQSLPFKIS
                     VVFLNACYSKHQARSISKYVDYIIGMKKAVSDEAAIIFSSKFYKSLLKNINYKEAYSD
                     AITYLSYYLESESSIPKLITSHGFDSISKIPKPTTTTVPKLLKQSEEKDDDNEELDDD
                     NEELVIQNLPVSADLLTQIEKSIRIYDEKSITAKFDFQKFWQNERDGNLSGILIILNN
                     NSSEYGVDDEQLRLIRNTLRNIPPIINKSEEYRDLINGYFDDIINQVLRKIFFYYKID
                     AIEKIQPLLPKISISYQNNDTLSKIMDTYYFLYKLKDQIEYSIMNYNNELFLIHSITQ
                     CYSILHIFNIRYNALIKAFKFISEISTNNYLSIQSLVSDLETSLENVKNISDLKKSKD
                     IILQIEKTTNELFTSFIQFIHLFMS"
     gene            5049..6458
                     /locus_tag="VF222_12995"
     CDS             5049..6458
                     /locus_tag="VF222_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7143385.1"
                     /translation="MTKLSMVTKYFVEVIASNKQQLINLQKFELDLFQPTAKSIDGKE
                     FKIDGLISLDDINKLVENGYKVFIKNESMKKTPAVQETTSFQDWSETAKKRKEVATRG
                     IKREEEETAAGEPSESLFTLSFTGYLTSEGIDSALQYIAKSYPALVQLIVLPEKTHEG
                     RTSRAIKISANNTTPKNGLLFLGGVHAREILNPDLLVKFALNLCEAYTANTGMKFEDK
                     LYSAEDIKQIVENLELFLFPLVNPDGRSFVQAPNGDVWWRKNKNPNPGLPQKGVDLNR
                     NYDFLWDSGIGTSTNSVTEIYRGKEAASEPETKNVLHLINKYQTISCVLDIHSYSELI
                     LYPWGDDENQIEDPDMNFRNQSYDGSRGHSGDSIYKEYIHKNDLDWYEATGKRIKNAI
                     VSVRGTVYESQPSVGLYPTSGTCDDYLHCLRYNGANRNIMGFTIETAKEFQPTYKVAT
                     NTMSEVTAGLIEVCKVHLE"
     gene            6822..7817
                     /locus_tag="VF222_13000"
     CDS             6822..7817
                     /locus_tag="VF222_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7143386.1"
                     /translation="MLKDIKNIEPDRLSQEELLKTRSAILKEIENRKNKTRGLDSIEE
                     RTTSSNELVEKLRSIEKVIYGVDDRKEAYEITNSQLKDGINSVVALIRNDNISDNGDG
                     KSTIRTRIFGVARNLCENEKFYNQPVAPFCSGFLVAKDMIATAAHCVENQADLKSIRF
                     VFGFRMKNETEGPMVIDNSDIYEGDNIVKRVFDDTGPDWAIIKLKRNVVGDHKPVILR
                     RSGKISDNESVYVIGHPVGLPTKFADGANVRNNNNESFFVCNLDTYGGNSGSPVFNSS
                     THELEGILVRGETDFIVTENGCRKSNVCPQNDPNMVCRGEDCTRSTEFVDFVPSN"
     gene            complement(7909..8301)
                     /locus_tag="VF222_13005"
     CDS             complement(7909..8301)
                     /locus_tag="VF222_13005"
                     /inference="COORDINATES: protein motif:HMM:NF036640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HEX7143387.1"
                     /translation="MTGHKPIEIFDIILLTNKNNLVEFKNNDEFYIEDVIVEDNILKL
                     NVSYSGGCKKHQFSLVEKKNLQRAKNIYEAHLQLIHNSNKDNCKKIVTEELFFNLLPL
                     KSKLQKENRVEKDISLVLIINNITVNYN"
     gene            complement(8400..9257)
                     /locus_tag="VF222_13010"
     CDS             complement(8400..9257)
                     /locus_tag="VF222_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143388.1"
                     /translation="MSINTDKLMNDIINLNKVTDNKHMLSEELLSEIITPEWYYSDSQ
                     RIFENTSISWDSLLRSEFTNNMSLNENRSTIMRRDIVFEKSFCTWYQPYHLLPRTKWG
                     IHIRSSSWMIVASQLNKECPHLISKLDESTVAAFLYIYLHMAFHYLVENAISTMELST
                     KNPSFYNDYYINNYLKTFNTPACKEESLANAYLYENASECHIDREYLKKELINQGDAY
                     ADFLKYTDNNNFFNGCQELIYKIKNEHKDLIDYEFENLEYFFNLRSLKFILQNQIPIW
                     LHHKPKPIH"
     gene            complement(9553..10200)
                     /locus_tag="VF222_13015"
     CDS             complement(9553..10200)
                     /locus_tag="VF222_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HEX7143389.1"
                     /translation="MRSANSKPRMAVVRDKLWDPGANIRIRFLNGEPSVQQKVQNYAT
                     RWMDHANLKLIFVEDGDADLRIAFEPDGSWSYIGRDALEIPQNEPTMNYGWLTSESPE
                     DEYSRVVLHEFGHALGAIHEHQNPAAGIPWNKEAVYRYYMGPPNNWPRDQIDNNLFRR
                     YSKNITNFTNFDSNSIMLYPIPAEFTTNGVAIGGRNSQLSELDIQFMKQQYPNIA"
     gene            complement(10862..11164)
                     /locus_tag="VF222_13020"
     CDS             complement(10862..11164)
                     /locus_tag="VF222_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143390.1"
                     /translation="MRAKRITAAPLHDGRLQLWGIFDDELFSCWKTTTNPDSAWTDWQ
                     HFPQPSHNITYINAAPLSDGRLQLWGIFDDKLFSCWKTTTNPDSAWTDWQPFPQPH"
     gene            complement(11511..12770)
                     /locus_tag="VF222_13025"
     CDS             complement(11511..12770)
                     /locus_tag="VF222_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143391.1"
                     /translation="MNASYSDNLYQVSYIPFSISTIKLLIQNEEYYDVDVLIKPTKFQ
                     NNDDFCGGFDDEKNGLKIIWGGGQENGLQTIIKGAEKKYLFLFIFPTENFNILTYNLT
                     LQVNKTRVESNTDNVSEEISITLHPIATGQLIHNIVHPKIMNNPENTKITFDGNLVNS
                     YYPRWYPELCNLFVNTDNNYYHSEPGVSNISFTLFKKSQGFDQDRIMLNFKGNEIAVI
                     EGDMDGASYHIDIFRDNNAFIVRLWFLWLSKKQFIDRNNLQPLQNDRKKRGIFDKPEL
                     PDFERFDIVISSHGTILAVCTDFHWQEYWYKLREDNTNMIGIVAKLFHPIDESLARLC
                     NRNFVENKYETIISDLQNITSLSERFHSKVKSIYVEGKEIEDSIQNRQKMPVVRGKNF
                     FRSHVPYVRNGYILSNMISHIVVDKYD"
     gene            complement(13647..14162)
                     /locus_tag="VF222_13030"
     CDS             complement(13647..14162)
                     /locus_tag="VF222_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7143392.1"
                     /translation="MKKLILIIPFEQSNDACGSAIDHSKELAQIVQNIISETGQNQVN
                     IVGFSKGGLDARVYLANDLANDNVANLIMIGTPNAGTQMAFTTNECSPGINDFKPNAL
                     ATKAKENTYTKYYTIAGTCFPWVGDGLVSTSSVNSQPYFKSLGTSNACHLELLDDYEY
                     ELAHDILRNIE"
     gene            14574..14795
                     /locus_tag="VF222_13035"
     CDS             14574..14795
                     /locus_tag="VF222_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143393.1"
                     /translation="MKSRIYRNKEITYSIPEDPHYVGKIDLEIEHKHVHALRLIDGTF
                     ATHFLPYLHYDSIEELTNEVIDKVPFYRS"
     gene            14824..15771
                     /locus_tag="VF222_13040"
     CDS             14824..15771
                     /locus_tag="VF222_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX7143394.1"
                     /translation="MQNIIIRKNVKSLSPTEKQHFIEAVKALKANTTESRIGDNKYDD
                     YVIWHAQTMMIAAGSDADANMRNLAHRGSVFLPWHREFLRRYELDLQKEVSDVTLPYW
                     DWAADASLRSPDPEIPSWTLSPIWTEDFMGGNGDPDNDDIVGNGPFKDWITQEVDDHG
                     NPSLKDRLRRDFGNIIRTLPTQIDVYNAFSFDFFDTAYWDVFSKGFSNALEGWINGPQ
                     LHNRVHVWIGGSMLINTSPNDPVFFVNHCNVDRIWALWQDLRFNFGYPSDGTIRDRNG
                     QRINHYNINDKIYPWQNEPDSKTIGDMLDYRKLGYRYER"
     gene            15987..17111
                     /locus_tag="VF222_13045"
     CDS             15987..17111
                     /locus_tag="VF222_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143395.1"
                     /translation="MYLFKINSIYSYTIFKCIIPFLFFYSLSNIFASPQFPEQEIIDD
                     TSDWIDLNSRNSTSAGDRYTDIVSIDYFSDGKSFNSTIWLLFPFKESPNRQYVDYGMY
                     IDSDFDPTTGFGGIDYKIEIQWNNSTQQWNKVIESWSPYGKTKVLYNATNYKNFYETL
                     ENYVTLSFDLDQLLNPKKYKILFYAETKKEEEQPYLVDFTRWVAIPPPQLIVSTSPNS
                     IDLIPGQKKTIEVRVNSSAGYEPTVNLFTQNRSNEILSSFLEYNRLRIPSYGMATTPL
                     TIATSPNSNPGPYTLFIFANTTFPAEELIKPRVSNMDYKSSIPSENIITQSTMLITIK
                     EPPSFLDQLSNFWAKSGGFLSFFYGIIIGITPFIYKKLKK"
     gene            complement(17195..>17520)
                     /locus_tag="VF222_13050"
     CDS             complement(17195..>17520)
                     /locus_tag="VF222_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143396.1"
                     /translation="SATEEESIPVPSQQPQFTHITKLIAEDLKKIPLEEIRTYPWNEL
                     GDEDVELVIKYLSYEPKFLGKILVNTPEDGISKFQNSLSVKTLNEIIANMPSEQVQLI
                     KDKFS"
BASE COUNT         6188 a         2428 c         2514 g         6390 t
ORIGIN      
        1 tcaaaatttg gaactcctgg gagtggtact ctagcaggac ttttaactga caacggaatt
       61 ttagaagagg aacttaccta tgacgatgta atgggcatga tagaaacaaa attagaaagt
      121 gttagtaata ccaccaatac gttagctcca agcagaactt taggagaaga acttaattct
      181 gaagtaatga acatgataga aacaaaatta gaaagtgtta gtaataccac caatacgttg
      241 gcacctgaat ctattcctat taaagacttc tatacaaaaa gtatactagg ggattcactt
      301 tccattgata ctgatggtgg tgcggatgat gataaagtag tagattcttc ctcatctgta
      361 tccgagactc tatcttcaat taccaaggaa gataacttaa taagtttatt tgatgaatct
      421 tttaatgaat atatagtttc accttttaat aaatatatag tttcttcatt aaattcttct
      481 tcatttattg ataaaataaa atcacataat tatgatatag attataatga aacaataaat
      541 aatttagagt ctcttaaaac aacattcgat tcgtctcttg gtggattagc agaggatgat
      601 ttaataactg atccaattga tcaacgaaat gtatattctg agattgatag tgcgattgat
      661 attttaaaag caaatagttg tttggaggac aattgtgttg tgttagaaaa acacccaaat
      721 gaaacattag tatattagac attctatcag ttcatttttt acaaaaatct ttgtaaatcc
      781 ttttcgagat aattatttaa atacttttaa ttaaaaggaa cgtattagct acgtttttat
      841 gaaagtttta aattgtgttt tattatcaat catcatctat attacaagat tgttaatgat
      901 aacaaaaaag gttaaataaa aataaacttt atttattatg tggtaaaagg tttagcacta
      961 actattggat tgaattttgt agatcctact cattatgcag gatgggatgg acagctaagt
     1021 gcatgtgagt atgatgcaga agatatgacc gatattgcaa agtctaaagg atttgaagta
     1081 caaaagttgc ttacaaaaac agcaacatct ggaaacgttg aaaatggtat tagtaaagct
     1141 gccaaaaaac ttgaatctgg tgatattttt ttattaagtt attctggtca tggaggaaaa
     1201 cttcctgata agaattctga tgaagtagac caaactgatg agacatggtg tctttacgat
     1261 cgtcaacttg ttgatgatga attaaataat tgtcttgcgc aattaacaaa aggagtaaga
     1321 gtattagtat tttccgatag ctgtcatagt ggaacagtta ccagagctat tgcagctaat
     1381 aaaaatattg atcaaatgaa ttcaaatatt gacaatcaag gaaaaaaata tagatttgct
     1441 cctaaaaatg tcattgcaag aacatataaa aataataaaa ctcaatatga caaaatcctt
     1501 actaatcgca atttaaagga atcagaagat aaagtaaaag cttcagttat acttatatct
     1561 ggatgtcagg ataatcaata ttctcaagat ggtgaattca atgggctttt tacatctaat
     1621 ttattggatg tatggaagga aggaactttt aagggatcgt ataaaagatt acataaatct
     1681 attattggac aaatgcctcc agatcaaaca ccaaactact atcttgtagg aacattagac
     1741 aaaaaatttg aaaaacaaat accttttaca atataaattt tttttattct attttttaaa
     1801 aatagagaaa aaatataata acaaacttta ctctgcaata ttttttttat tgttatctca
     1861 tagatttatc tagtcataga tctaggtatt taaatttcta ttttatttag caagaatgat
     1921 tttatggtat ttcttgaata cttttttatt gtcaagaaga atgttagcca ataggttaaa
     1981 gtttctccat ccttttcctt ttaacaaaaa tccattttcc tgggtaaata cagtcttttt
     2041 agactcttta tacgttggat ccaatacata ttctactgat attatagact gcataacctc
     2101 aggaggtgct tttacacata gttcccaata ataccatggt tcatgctgtt tatctactat
     2161 tgatttactt gcaatcttaa ttttttcaga tccaatttgc tgctgatttg ctaattttct
     2221 aatttgttgt aaaatttgtt ccttattact ctcattgatt ttcggtatag tcagtattaa
     2281 agacaaaagc tttcttagtt taatatcaat tttgctatta ttaacatgac ttattaaatt
     2341 gttaaaattc caatgaacaa gctctaaatt ctttagttca caaaattcca ggagttcatc
     2401 tacaaagatt ttggaaatct tctcttgaga taaagcttgg aaacatatca ataaaattct
     2461 taatatgaga gaggtagacc tatcttcaat tccttctagt ttttttaaaa tctcttttaa
     2521 atgtctttca ctttcattat atttattcag taatatcaaa acttgcgata aattcatttt
     2581 agctactatt gaatttggat tcaatgcgat acttttatta aaaatatctt tagcttcttc
     2641 atatttataa aaaccatcac ttaatacaca accgtacgct aataacaacc tccaatcatt
     2701 attttgaatc ttcatagcat cttctaactt taattttgca ttctcataat cttcggtttt
     2761 atagaataat aatcctaagg atattaatga ttcatagtgt tgcggcttga ttgttaaagc
     2821 ccgttcatat aatattatag ctgaggtctt atagaaatta ttaagataat cgctggtgtt
     2881 ccataacatt tcatacatca aagcagaatt atacaataat agaacgttat tttcatctag
     2941 ttgcattgca ttaccaaaac atcttaaagc atcatcaaat ttatgactat gaaaataaga
     3001 cactcctaac ttatttaaaa tatcaatatt atttttttca tactctaaag catttttaaa
     3061 ttttgaggag gcatgttcat atttttcttc taaaagaagc atgtttccct cctgtatatt
     3121 taatttcatt aattcctttt gtataatttg ttgattaaat ttccaatatt catatgagtc
     3181 tctaattgaa gaagaaagtt cagataattc cgaatcttgg ataataatag tcatagcttt
     3241 ctcatatcct aaagtaattt ctctatagaa ttgcaatatt ggattcagat cattgtttcg
     3301 ataaaaatag ttacataagt caattgttaa ttttataatt tcatttaata attttagata
     3361 gttttttata tctttttttt cctcttttag cttgtttagc aatattgtta tttgatcata
     3421 ttttgactgc attagtgaat gttaatgtta taggtaaaag acctttaaaa atattattat
     3481 gacataaata aatgaatgaa ctgtataaag gaagtgaata attcatttgt tgttttttca
     3541 atttgtaaaa taatatcttt actttttttt agatctgata tatttttaac attttccaat
     3601 gatgtctcaa gatcagaaac taaagattga attgataaat aattattagt agaaatttca
     3661 gatataaatt tgaaggcctt aattaaggca ttgtatctaa tattaaaaat atgaagaata
     3721 ctatagcatt gtgtaataga atgaattaaa aaaagttcat tattataatt cattattgaa
     3781 tattcaattt gatcttttaa tttatataga aaataatatg tatccattat ttttgacaat
     3841 gtatcattat tttgataaga gatagatatc tttggaagca aaggttgaat tttttcaatt
     3901 gcgtcaattt tgtaataaaa aaatattttt cttaatactt gattgattat atcatcgaaa
     3961 taaccgttga tcaaatctcg atattcttct gatttattaa ttattggtgg tatatttcgt
     4021 aaggtatttc tgataagacg gagttgttca tcatcaacac catactcact tgaattatta
     4081 ttcaaaataa tcaaaattcc agataagttt ccatcccttt cattttgcca aaatttttga
     4141 aagtcaaatt tagccgttat acttttttcg tcatatattc taatactctt ttcaatttgt
     4201 gtcagtaaat ctgcagatac tggtagattt tgaattacta gttcttcatt atcatcatct
     4261 agttcttcat tatcatcatc tttttcctca gactgtttta gaagtttagg tacagttgtt
     4321 gttgttggtt taggtatttt actaatagaa tcgaatccgt gggaagtaat tagtttggga
     4381 atggatgatt cactttcaag ataatagctc aaataagtaa tagcatcgct ataggcttct
     4441 ttataattga tattttttag aagagattta taaaattttg atgagaatat tattgcagct
     4501 tcatcggaaa cagctttctt cataccaata atataatcta catattttga aatagatctt
     4561 gcttgatgtt ttgagtagca tgcatttaaa aaaacaacag atatcttgaa cggtaaactt
     4621 tgaaaggttt tctctaattg ttccgatgaa agttcatcac catcttgaaa aacaagtgaa
     4681 gaggactcag taccatgacc agaaaagtgg agaatatttg gtctatagcg ctcaagatca
     4741 tctagaaaat gatcctttgt tattcccaga atgtgaaaaa attgaaattt atctctttga
     4801 ccatcaagtg tttgttgtat ttctgtacct tctttaacga catttacata tattttatca
     4861 tcaggagctg ctgccataaa taatacagtt attttagaac tcaaattaca agatctaata
     4921 tactattgta tatcaaaata taaagattta tcgagagtag ttattgtctt tttattacaa
     4981 attattaatt tttcggcgcc gcaaaattcc aataatgaga tatataccaa aactcttaag
     5041 attctgcctt gacaaaatta tctatggtaa caaaatattt tgttgaagtt atagcatcaa
     5101 ataaacaaca attaattaac cttcagaaat ttgaattaga tttatttcaa cctacagcaa
     5161 aatccattga tggtaaagaa tttaaaatag atggattaat ttcattagac gacataaata
     5221 aactagtaga aaacgggtac aaagtattta taaaaaatga gtcaatgaaa aaaacacctg
     5281 cagttcaaga aactacgtcc tttcaagatt ggagtgaaac agctaagaaa cgtaaagagg
     5341 tagcaacaag aggaataaaa agagaggaag aagaaacagc agcaggagaa ccatcagaat
     5401 ctttattcac attatcattt acaggatatt tgacttcaga gggaatagat tctgcattgc
     5461 aatacatagc caaatcatat cccgcactag tacaattaat cgtgcttcct gaaaagacac
     5521 atgaaggtcg aactagtaga gcaataaaaa taagtgcaaa taatactact cctaaaaatg
     5581 gtttgctttt tttaggagga gtgcacgccc gagaaatttt gaatccggat ttattagtaa
     5641 aatttgcctt gaatttatgt gaagcctata cagccaatac aggaatgaaa tttgaagata
     5701 aattgtatag cgcagaggat ataaaacaaa ttgtagagaa tttggaactt ttcctgtttc
     5761 cattggtaaa ccctgatggt agatcatttg tccaagcacc caatggagat gtatggtgga
     5821 gaaagaataa aaacccgaat cctggtcttc cacaaaaagg tgtagatcta aatagaaatt
     5881 atgattttct ttgggatagt ggtataggta caagtacaaa ttcagttaca gaaatttaca
     5941 gagggaagga agcagcttca gaaccagaaa caaaaaacgt tttacattta ataaacaaat
     6001 accaaactat cagttgcgtg ttagacatcc actcttattc tgaactaatt ctttatccat
     6061 ggggggatga tgagaatcag atagaagatc ccgatatgaa ttttagaaat cagagttacg
     6121 atggatcacg tggtcattca ggagatagta tatacaaaga atatattcat aaaaacgatc
     6181 tggattggta tgaagctaca ggtaaaagga ttaaaaatgc gattgtatcc gtaagaggaa
     6241 cagtttatga atcacagcct agtgtaggtc tttatcctac atcaggtacc tgtgatgatt
     6301 atctacactg cttaagatat aacggagcta ataggaacat aatgggattt accatagaaa
     6361 cagcaaagga atttcaaccc acttataaag tggcaacaaa tacaatgtct gaagttacag
     6421 ctggtttgat agaagtttgt aaagtacatc ttgaatagtc ttttccattt ttatttctct
     6481 ttatgtttct aatataaagc cattatagga tttttattga taataatctt ttagtgagaa
     6541 aatttatcaa aatgaagatc aagaatgatt tgtagtaaga atatataatc tatatttttt
     6601 gaaactattt ttgaagctgt ttagtaaagc taatcgatta tctcgttata tttaattaaa
     6661 ataaattaag ggtgtcatta tttgatatca cttttaaaag catttgaatc aggatacaat
     6721 ttttaacttc attctattgt ttgtttgtat tctacattat attatttatt ctaaaaatat
     6781 agtaacagaa attttttata gtgttaagta gattcatgtc tatgttaaaa gatattaaaa
     6841 atatagaacc cgatcgacta tctcaggaag aactattaaa gactagaagt gctattttaa
     6901 aagaaataga aaatagaaaa aataaaacac gaggtctaga tagcattgaa gagcgaacta
     6961 catcttctaa tgaacttgtc gaaaaattac gctctataga aaaagtcatt tatggtgttg
     7021 atgatagaaa agaagcttat gaaatcacta attcacaact aaaagatgga attaatagtg
     7081 ttgttgcact tattcgtaat gacaatataa gtgataatgg tgatggtaag tcaactatta
     7141 gaacgagaat ttttggagtc gcaagaaatt tatgtgaaaa tgaaaagttt tataatcaac
     7201 ctgtagcacc attttgttct ggttttctag tggcaaaaga tatgatagca actgcagccc
     7261 attgtgttga aaatcaagca gaccttaaat ctattagatt tgtttttggg ttcagaatga
     7321 aaaatgaaac ggagggacca atggttattg ataattcaga tatctatgag ggagataaca
     7381 tagtaaaaag agtgttcgat gatactggac cagattgggc aataatcaaa ctaaaaagaa
     7441 atgttgttgg tgatcacaaa ccagttattt taagacgatc tggaaagata tcagacaacg
     7501 aatcagttta tgtcattggt catccagtgg gattgccaac aaagtttgca gatggtgcaa
     7561 atgttagaaa taataacaat gagtcatttt ttgtttgtaa tcttgacaca tatggaggta
     7621 attcaggttc accagtattt aacagctcta ctcatgaatt agaaggaatt ttggtacgag
     7681 gtgaaactga ctttattgtt acagaaaatg gatgcagaaa atctaacgta tgccctcaaa
     7741 atgatccaaa tatggtttgt agaggagaag attgtacaag atctacagaa tttgttgatt
     7801 tcgttccatc caattaattt ttttaaatga ggcaaggata gagccattat ttccaatcca
     7861 attattttat attattatgt actagaattt ctactattgt agacaaaact aattataatt
     7921 tacagttata ttattaataa ttaaaactaa actgatatct ttctcaactc tattttcttt
     7981 ttgaagctta gattttaatg gtaaaagatt aaaaaataac tcttctgtaa ctattttttt
     8041 acagttatct ttattagaat tatgaattaa ttgtaaatgt gcttcataaa tatttttagc
     8101 tctttgcaag tttttctttt ctactaatga gaactggtgc tttttacaac caccactata
     8161 agacacattt agttttaaga tattgtcttc tacgattaca tcttcaatat aaaattcatc
     8221 attatttttg aattctacta aattattttt atttgttaat aatattatat caaaaatttc
     8281 gataggttta tgacctgtca taatgatatg taattagtca tatattaaaa tttaacttag
     8341 actatcttat aatcgtacta atgtaaaaag attaattaaa ataataatgt aatctttggt
     8401 taatgaatag gtttaggctt atggtgtaac cagataggaa tctggttttg taatataaat
     8461 ttaagagatc ttaagttgaa aaaatattcc aaattttcaa attcataatc tattaaatcc
     8521 ttatgctcat tcttaatttt atatattaat tcctgacatc cattaaagaa attattatta
     8581 tcagtatatt ttaaaaagtc agcataagca tccccttgat ttatcaattc ttttttaaga
     8641 tattctctat caatatgaca ttcggaagca ttttcataaa gatatgcatt tgcaagagat
     8701 tcttccttac aagctggtgt attaaatgtc tttaaatagt tatttatata ataatcatta
     8761 taaaatgatg gatttttggt tgatagttcc attgtactaa tagcattttc tactaagtaa
     8821 tgaaacgcca tatgaaggta aatatataaa aaagcagcaa cagtagactc atctaactta
     8881 gatattaaat gtggacattc tttatttaac tgagaagcaa ctatcatcca actagatgat
     8941 cgtatatgaa ttccccactt tgttcttggt agtaaatggt atggttggta ccaagtacaa
     9001 aagctttttt caaagacaat gtcacgccgc ataattgtag atctattttc atttaaggac
     9061 atattattag taaattctga tcgtaataat gaatcccaac taatacttgt attttcaaat
     9121 attctctgac tatcagaata ataccattct ggagttataa tctctgacag taattcttct
     9181 gatagcatat gtttattgtc tgtaacttta tttaaattta taatgtcatt cattaacttg
     9241 tctgtattaa tagacatatg tgaatatact attgcatttg gcagttatat agttagtctt
     9301 gataaataag tttaatattt aaagatgtaa atgcaaagga tattatatta tattaacaag
     9361 agtaatttaa tctaaaaact ttgaatagta aaagaaatat tctgttaatt atgattagga
     9421 attttctaaa aattttaccc aaacaaatat aataatagta atattattat tattattatt
     9481 atttaataca gaacttttaa aaaaaatata ttgaattagc tatttgatat gtagatagat
     9541 atatagatat tgttaagcta tattaggata ttgttgcttc atgaattgta tatctagttc
     9601 ggacaattga gaatttcttc cacctatagc gacgccattt gtagtaaact cagcaggtat
     9661 tggatatagc ataattgaat tgctgtcaaa gttagtaaaa ttagtaatat ttttgctata
     9721 gcgtctaaaa agattattat caatttgatc tcttggccaa ttatttggtg gacccatata
     9781 atatctgtat acagcttctt tattccatgg tataccagct gctggatttt ggtgttcatg
     9841 aattgcccct aaagcatgac caaattcatg tagcacaact ctagagtatt catcttctgg
     9901 actctctgat gttagccaac catagttcat agtaggctcg ttttgtggta tttctagtgc
     9961 atctctaccg atatatgacc atgatccgtc aggttcaaaa gcaattctta aatctgcatc
    10021 tccatcttca acaaagatca attttaaatt ggcatgatcc atccatcgtg ttgcataatt
    10081 ttgtactttt tgttgtacac tgggttctcc attaagaaat ctaattctaa tattagcacc
    10141 tggatcccat aatttatctc tcacgacagc catccttggt ttggaattag ctgatcgcat
    10201 aatattctcc ttgttttcct tatatttaat tggctcattt ccgtttaatg ttttgtcaat
    10261 acaaaccttt atcataaata ataagaaagt tctattacta tttattaatg atcctaattt
    10321 ataaattatt ttttaacaca taatatttta gtatttatga ataaggccaa accaatttta
    10381 gttattgatt atatttaata tcttctttta ggaacaagat ccttcccatt aatactcaaa
    10441 taattaacta atattagaga ttgatttgta ttaatcatta caaatcagaa tttcattttc
    10501 aacagttagg aaaactaatg atcacttata gaattgaaat agctttaaat attataaata
    10561 aatttaatag aaatagcgat acgattaata gtttctatat gaatatcatt ggcacttaag
    10621 ctggttatat ctaattgttt tgtattttga ctgtttaaac ccattatata atttgaagaa
    10681 attatgcaaa ctattccatt gaatattcat cataatttat tgcaacattc aaaatgttca
    10741 atttttagct gattttacca agtatatttt tttagtttga gagactacgt ataaatggaa
    10801 tataatctag ttagctaatt tatcaatcac atagtgaaac catagttatt tttgtgccaa
    10861 attagtgagg ttgaggaaaa ggttgccaat ccgtccatgc tgaatctgga ttagtagtag
    10921 ttttccaaca actaaaaagt ttgtcgtcaa aaatacccca taactgaagt cgtccatcag
    10981 atagtggagc tgcatttatg taggttatat tatgcgaagg ttgaggaaaa tgttgccaat
    11041 ccgtccatgc tgaatcggga ttagtagtag ttttccaaca actaaaaagt tcgtcgtcaa
    11101 aaatacccca taactgaagt cgtccatcat gtagtggagc agcagtaata cgttttgccc
    11161 tcattacctt ttttgccctt cctttgtgag atattcgtac tgcgttattg tatattgaat
    11221 aataatgttc atgtctaaat tagagacatt tgtagtattt aagattattc catatgacta
    11281 taaaatatta acaaaatcac ttaattggtt tgattgaaag caaaataata taatatatat
    11341 taaattttga aatgccaaaa aaaatcttct ttaatttaac ttataacatt ctatggttgg
    11401 attgaataat tctttatgtt gaaagtgtat ctagacatat ttttctttgt atataatata
    11461 tccattagtt taatatatta aatgttacta acaaaataag aattaatcag ttaatcatat
    11521 ttgtctacta ctatatgaga aatcatattt gataatatat aaccatttct tacataaggt
    11581 acatgacttc tgaaaaaatt ttttccacgt acaaccggca ttttctgtct attttgtatt
    11641 gaatcctcta tttcttttcc ttctacatat attgatttaa ctttagagtg aaacctttct
    11701 gataatgatg ttatattctg taaatcacta ataatagttt cgtatttatt ttctacaaag
    11761 ttacggttac ataaacgtgc taacgattca tctatcggat gaaataattt agcaactatc
    11821 cctatcatat ttgtattatc ttctcttagt ttataccaat attcctgcca atgaaaatct
    11881 gtacatactg ccaaaatagt accatgggaa gaaattacaa tgtcaaatct ttcaaaatca
    11941 ggtaattctg gcttatcaaa aattcctctt ttttttctgt cattctgtaa tggttgaagg
    12001 ttatttctat ctataaattg ttttttacta agccataaaa accataatct tactatgaaa
    12061 gcattattat ctctaaatat atctatatga tagcttgctc catccatatc gccttctatg
    12121 accgcaattt catttccttt aaaatttaac attattcgat cttggtcaaa tccttgtgac
    12181 tttttaaaaa gtgtaaaaga aatatttgat accccaggtt ctgaatgata ataattatta
    12241 tcagtattga caaataaatt acataattca ggataccatc ttggataata agaattcact
    12301 aaattaccat caaaagttat tttagtattt tcgggattat tcattatctt tggatgaaca
    12361 atattatgta ttaattgtcc agttgcgatg ggatgtaatg taatacttat ttcttctgat
    12421 acattgtctg tattcgattc tactctggtt ttatttactt gtaatgttaa attgtaggtt
    12481 aatatgttaa agttttctgt aggaaaaata aaaagaaata aatatttttt ctcagctcct
    12541 ttgattattg tttgtaaacc attttcttga ccccctcccc agataatttt aagcccattc
    12601 ttttcgtcat caaaccctcc acaaaaatca tcattatttt gaaattttgt aggttttatg
    12661 agaacatcaa catcataata ttcttcattt tggatcaata atttaatggt agaaatcgaa
    12721 aaaggaatgt aactaacctg ataaagatta tcactatatg aagcattcac aatttttaca
    12781 ctacaattaa tcaacaccat tgaaagtttt ttgtagtata aaaaatttat tatataaaga
    12841 ctgaattgac attagtacaa ttatgtattt tctaaggtta gtatataatt ttctaactat
    12901 agaattttta tttttcaatt aattacctga tatcgtaaaa tgattattca ttaactatat
    12961 aataactaaa cctcttgttg aaaggaaata ttttatcact aaatgataca tcaatttagc
    13021 ttaaaagatt tttttcttct tcaatttcat cctactctat tatttttttt cctcgctggt
    13081 ttagatcata gaaatcggtt atgcagacaa agatggttgt catagatggc atagttgtcc
    13141 ttctgatagt ggtagttatg tttgtggtaa tgttggttat aatgatgaat gttgaatgaa
    13201 ggactacaca gactttcaat ttgaccaaaa ttgataaata tattaaattg atttaaaatg
    13261 agatattgat gtcgtttcca ataagatgaa aaagatcatg gctagtcctt ttcatacttt
    13321 tatcattaaa tcattttttt gtatatcatt ataattatat gattttagaa ttatttttat
    13381 atttggtgat tagatttata gaataaagaa attttaaata ctcacataga agtgacattt
    13441 attaaatgaa atttgttatt atttaattta atttatttta aaagttgata attgtattta
    13501 ttctatattg ataaaattag tttttgttta atatatcaat taaatttgta taattaagtt
    13561 ttttttaata cgttatagtt gacgagagat tctattaaat aatatattta aaagaaaata
    13621 ctatttcttt taaattaaaa ttattcttat tctatatttc tcaatatgtc atgtgctaat
    13681 tcatattcat aatcgtctaa caactctaaa tgacaagcat tagaagttcc aagggatttg
    13741 aaatagggtt gtgaattaac actggacgtg ctcactaatc catcacctac ccaaggaaaa
    13801 catgtaccag caatagtata gtacttcgta tatgtatttt ctttggcctt agtagcaaga
    13861 gcgtttggtt taaaatcgtt tatacctggc gaacattcat tggttgtaaa tgccatttga
    13921 gtacctgcat taggtgtccc gatcattatt aaatttgcaa cattatcatt agcaagatca
    13981 ttggccaagt atactcttgc atctaaccca cctttactaa aaccaactat atttacttga
    14041 ttttgtcctg tttcactaat gatattttga actatttgag caagttcttt agaatggtct
    14101 atagcagaac cacatgcatc atttgattgc tcaaaaggta ttattaaaat taattttttt
    14161 atatatttta atgcttaata ggacttaata gaattaccaa atataataaa taataccaat
    14221 accatataat catatcatta cacaagaaaa atgctgtaat gataatagga tggatgaatc
    14281 tgagctatat ctttccatct tattggataa cgtcatcaaa cattcttatt ttttattttt
    14341 cccacctgaa aattactatc ggctaattcg ctctcattta gtccatctgc ctataaaatc
    14401 attattggtt gatttagatt tgagaaaaca aaatattatt agaagaaaag tcataatgat
    14461 agtacatagt agaattttaa attattttat aattaatatt tttgtctaac taagatatta
    14521 acaagtagtt atcataaaaa attttataaa tcaataggaa caaattgatg ttattgaaat
    14581 caagaattta tagaaataaa gaaataacat attcaattcc tgaagaccca cattatgttg
    14641 gcaaaattga tttagaaatt gagcataaac atgttcatgc attgagactt attgacggca
    14701 catttgcaac tcattttctt ccatatttac attatgattc gatagaagaa cttacaaacg
    14761 aagttattga taaagttcct ttttatcgtt catgaaataa aataaaaaaa agagtcttat
    14821 aaaatgcaga atataataat cagaaaaaat gtaaaatcat tatctcctac tgaaaaacaa
    14881 cattttatag aagcagttaa ggcactaaaa gcaaatacaa cggaaagcag aataggagat
    14941 aacaaatatg acgattatgt aatatggcat gcacaaacaa tgatgattgc tgctggctct
    15001 gatgctgatg ccaacatgcg taatttagct catagaggat cagtattttt accttggcat
    15061 cgtgaattct tacgtagata tgaactagat ttacaaaaag aggtatcaga tgtaacatta
    15121 ccatattggg attgggcggc tgacgcaagc cttcgatctc ctgatcctga aataccatct
    15181 tggacactat ctcctatctg gacagaagac tttatgggcg gaaatggtga tccagataat
    15241 gatgatattg taggtaatgg cccttttaaa gattggataa cccaagaggt tgacgatcat
    15301 ggaaatcctt cattaaaaga tcggcttaga cgcgattttg gtaacataat cagaacctta
    15361 ccaactcaaa ttgatgtata taatgcattt agctttgatt tttttgatac tgcttattgg
    15421 gatgtcttca gtaaaggttt tagtaatgcc ttagaaggct ggataaatgg tccacaactt
    15481 cataatcgag ttcatgtatg gattggtgga tcaatgttaa ttaatacttc tcctaatgat
    15541 ccagtatttt ttgttaatca ttgcaatgtt gatcgtatat gggcactatg gcaagattta
    15601 cgattcaatt ttggctatcc ttctgatggt acaataagag atagaaatgg tcaaagaata
    15661 aatcattata atataaatga caaaatatat ccttggcaaa atgaacctga tagtaaaact
    15721 ataggtgata tgttagatta cagaaaatta ggatacagat atgaaagata ataaattatc
    15781 tagaatacca gattaatcgc caatatacat atatacatac ccacataact tatattctta
    15841 gtttttgagt aagattatta gtatgttata atttatatta gaaaattaac ttagaataat
    15901 tttttatcaa attttgcggc gccgaaaaaa tcaatctgca aagaagcctt aataatataa
    15961 tcttgtcaaa taattcagat aattagttgt acctatttaa aatcaatagt atatattcat
    16021 atacaatttt taaatgtata ataccttttt tattctttta cagtttatca aacatttttg
    16081 cttcacctca atttcctgaa caagaaatta ttgatgatac atcagattgg atagacctta
    16141 attcaagaaa ttctaccagt gcaggtgatc gttataccga cattgtatct attgattatt
    16201 ttagtgacgg aaaatctttt aattctacta tatggttact gtttcctttt aaagaatctc
    16261 ctaatcgtca atatgtagat tatggaatgt atattgattc tgattttgat ccaacgactg
    16321 gatttggtgg aatagactac aaaatagaga tacaatggaa taatagtaca caacaatgga
    16381 ataaagtaat agagtcatgg tcaccttatg gaaaaaccaa ggtattgtat aatgctacta
    16441 actataaaaa tttctatgaa acacttgaaa attatgttac gttgtcattt gatcttgacc
    16501 aactattaaa ccccaaaaaa tataaaattc ttttctatgc cgaaacaaaa aaagaagaag
    16561 aacaacctta tttagttgat tttactagat gggtagcaat tcctccacca cagttaatcg
    16621 tttcaacgtc gcctaattct attgatttaa ttccaggtca aaaaaaaact attgaagtga
    16681 gggtaaattc atctgctggt tatgagccta cagtaaatct ctttactcaa aatcgatcca
    16741 atgaaatatt aagttctttt ttagaatata atagactacg tattccctcc tatggaatgg
    16801 ctactactcc acttactatt gcaacttctc caaatagtaa tcctggtcca tataccttat
    16861 ttatttttgc taatacaaca tttccagcag aagaactaat aaaaccaaga gtatctaata
    16921 tggattataa atcttctatt cctagtgaaa atataataac ccaatccact atgcttataa
    16981 ctattaaaga gcctccatct tttcttgacc aattaagtaa cttttgggct aaaagcggtg
    17041 gtttcctatc ttttttctac ggaataataa ttggaataac accttttata tacaaaaagt
    17101 taaaaaaata atcatttaat tttataatga acaaaacact actaatggta aaagtgttat
    17161 tctaatatcg ttattagtta atgtttttta tcacttacga aaatttgtct tttattaatt
    17221 gtacttgttc agaaggcata tttgcaataa tttcattcaa agtttttact gatagactat
    17281 tttgaaactt gcttattcca tcttctggtg tattaaccaa gattttacct aaaaactttg
    17341 gctcatatga taaatattta ataacaagtt ctacatcttc atcacctaat tcattccaag
    17401 gatatgttct aatttcttct aatggtattt tttttagatc ttctgcgatt aattttgtaa
    17461 tgtgcgtaaa ttgtggttgt tgggatggaa ctggtatact ttcttcctct gttgctgaag
//
LOCUS       DASVEV010000300         5271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1525322, whole genome shotgun sequence.
ACCESSION   DASVEV010000300 DASVEV010000000
VERSION     DASVEV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525322"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF222_13055"
     CDS             <1..420
                     /locus_tag="VF222_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7143397.1"
                     /translation="GVKDKKIITEIVIESLEGAGLWQEVKDELNKPGMSLSGGQQQRL
                     CIARALAMHPEIILMDEPTSALDPRASSKIEELIHELKKYLTIVIVTHNMQQAARVSD
                     YTAFMYLGELIEFGKTKQIFEKPKKEFTERYISGKFG"
     gene            608..1441
                     /locus_tag="VF222_13060"
     CDS             608..1441
                     /locus_tag="VF222_13060"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7143398.1"
                     /translation="MTLLSFLIKLHENLRRFDSKTNSYIKNSNEKNIHDVRTSIRRFN
                     AAFLILPKRYRKGCLLSDYNQLANKFFKVNSEIRDMDIIHEKLNGYPQTDQRNIVIDL
                     LKKNRQSNLEGAKTIALSLKNMNSNKILDKMDITEKEVQKRYNKLLFRLISKIETNFL
                     IVITNSLKIKELHELRKDCKKLKYILELLEKDNNKNNTAIGLIKTLQNIQDILGSIRD
                     YDITIDYLKKLESSKEIQELVNNETEQRRLKYEEFLRFCKRRLRISRDSFFARIKSLL
                     S"
     gene            complement(1513..2619)
                     /locus_tag="VF222_13065"
     CDS             complement(1513..2619)
                     /locus_tag="VF222_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7143399.1"
                     /translation="MTITKKKMYYTIIGFAMMIFMASNFSPELSYSEDKFSKSYIAEN
                     SNILENNKKYYSVDNSIEDFNFVAVGDFSCNEDAKNTIINIINTDPEMFLGLGDYSYE
                     TSAKCWKDIVGGIDPQKMKISFGNHEFEGQSLLKQYMEYTNLDNQYYSFDYNNVHFIS
                     MATEAPYEQGSEQFDFVKHDLMRTKTNSSIDWIIVYYHQPMYTSKTHHEGLESFRDLY
                     HGLFNEYGVDLVLQGHVHNYQRTHPLTYNSESPSNPTISQSAILDNGNNEENMYVDPE
                     GTIFAIVGTGGRELHGLDDQAYFTANQLEEHGFLELKTTNNGKSLVGQFYSNEGSIIK
                     DEFIIEKSSVDKTFNDNTPNNNPFNLNEITYKNK"
     gene            3576..4940
                     /locus_tag="VF222_13070"
     CDS             3576..4940
                     /locus_tag="VF222_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX7143400.1"
                     /translation="MFISTTKNETISIFAQNMTNQTEYLNTTESFLTKTKPYLQVISP
                     SNSTVKSLITFNDILPNGYIFPRLPDGLASIHNRDGLIDIFVNHELALDKVNEYAKVS
                     KLTVDENGRIISGELIEDGSGKYERFCSATIFMENGFKNPLFVTNEEIEEGIVVAYDT
                     VTKNKTEMPWLGKFSHENTILVPNKNNKTIVLTTEDGDPNHSQLYMFISDNPTNFLNG
                     IGKLYVLIGENNITSFQDLQKGIIYNGHFQPVTWNWMTQNSTDLEKEVQNLNALDFMR
                     LEDTDYNKVNNSVIYITDTGSDEFGERYKDGRLYQFEIIEPSLNQANNTNVTNNNNYY
                     KVKISILLDGGDGGDDLRNPDNIATSKKSIMIQEDLNDYNIIDGGVNARVLKYDLTSH
                     NLDPVAIVDQSPDVSHPKAGEWESSGIIEVFDIFGKGYWLLDIQAHTLGEGGQLLLMN
                     SPGS"
BASE COUNT         2009 a          701 c          750 g         1811 t
ORIGIN      
        1 ggtgttaaag ataaaaaaat cataacggaa atagtaatag aaagtcttga aggtgccggc
       61 ttatggcaag aggtaaaaga cgaattgaat aaacccggaa tgagtctgtc aggtggccaa
      121 caacaaagac tttgtattgc gcgcgcactt gcaatgcatc ctgagattat tcttatggat
      181 gaacctacat ctgccttgga tcctcgtgca tcttctaaaa ttgaggaact aattcatgaa
      241 ttaaagaaat accttactat agtaatagta acacataaca tgcaacaagc agctagagtt
      301 tcagactaca ctgcatttat gtaccttggt gagttaatag aatttggaaa aacaaaacaa
      361 atttttgaaa aaccgaaaaa agaatttact gaaagatata tctctggtaa atttggttaa
      421 ctatctataa taatcaatta tattgtccaa tattaaattt attaaattcg gaaatagaaa
      481 gaaaataggg aattaaacca taagtttctg aaacttggat aaaaaaaaat aaataattat
      541 aaaagataga aaaaattttt tacatattaa ctttataagt acaataactt aatagaacat
      601 tatcttgttg acactattat cttttttaat aaaactacat gaaaacctaa gaagatttga
      661 tagtaaaact aatagttata ttaaaaactc caatgaaaaa aatattcatg atgtaagaac
      721 ttcgatcagg agatttaacg ctgctttttt aatattacct aaaagatata gaaaaggatg
      781 tttattatca gactataatc aactagctaa taaattcttt aaagtaaata gcgagattag
      841 agatatggat attattcatg aaaaattaaa tggttatcca caaactgatc aaagaaacat
      901 tgtaattgat ttattgaaaa agaatagaca atctaattta gaaggtgcaa aaactattgc
      961 tttatctctt aaaaacatga attcaaataa aattctggat aaaatggaca taacagaaaa
     1021 agaagtacaa aaaagatata ataaacttct ttttagacta atttcaaaaa tagagacaaa
     1081 ttttctcatt gtcataacaa attctttaaa gataaaagaa ttacatgaat taagaaaaga
     1141 ttgtaagaaa ctgaagtata ttttagaatt attggaaaaa gataataaca aaaataatac
     1201 tgctattgga ttgataaaaa ctcttcagaa tatccaagat attcttggga gcattcgtga
     1261 ttatgatatc actattgatt atttaaaaaa attagaatct agtaaagaaa ttcaggaatt
     1321 agttaataat gaaacagaac agagaagatt aaagtatgaa gaatttttaa gattttgtaa
     1381 aagacgttta cgtatctcca gagattcatt ctttgcaagg attaaaagtt tactttcata
     1441 ggcgagtaaa tggtttttct atatatgtga agtaaaaatt atagtgtatg taattaaaaa
     1501 gtcaattaat ttctatttat ttttatatgt aatttcattc aagttaaatg gattattatt
     1561 tggggtattg tcattaaaag ttttatcaac agatgatttt tcgataataa attcgtcttt
     1621 aattatacta ccttcattag aataaaattg tccaacaaga ctttttccat tgtttgttgt
     1681 ttttaattcc aaaaatccat gttcttctaa ttgatttgca gtgaaataag cttggtcatc
     1741 taatccatgt aattcccgtc caccagtacc aactatggca aaaatggtgc cttcgggatc
     1801 aacatacata ttttcttcat tatttccgtt atctaatatt gcgctttgtg atattgtagg
     1861 attggaaggg ctttcactat tatatgttaa aggatgtgtc ctctgataat tatggacatg
     1921 tccttgtaat actaaatcta caccatattc attgaaaaga ccatgatata gatctctaaa
     1981 ggattctaaa ccttcatgat gagtctttga agtatacatc ggttggtggt agtaaacaat
     2041 tatccaatct attgaagaat ttgtttttgt cctcattaaa tcatgtttaa caaaatcaaa
     2101 ttgttctgat ccttgttcat atggtgcttc tgtagccata gagataaaat gtacattatt
     2161 ataatcaaag gaataatatt ggttatcaag attagtatat tccatatact gctttaataa
     2221 tgactgacct tcaaattcat gattaccaaa cgaaattttc attttttgag gatctattcc
     2281 tcctacaatg tccttccaac atttcgcact tgtttcataa gaataatctc ctaaacctaa
     2341 gaacatttca ggatctgtat ttattatatt tataatagta ttttttgcat cttcattaca
     2401 actaaaatct cctactgcta caaagttgaa atcttcaata gagttatcca ccgaataata
     2461 tttcttatta ttttctaaaa tatttgaatt ttccgcgata taagatttac taaatttatc
     2521 ttctgaatat gataattcag gagaaaaatt acttgccatg aatatcatca tagcaaaacc
     2581 aattattgtg taatacattt tttttttagt tatagtcaaa ttaatcactt gtgatatagt
     2641 atagtcaagt atatcataaa agagacatga tcatagataa cttgtcaata tacacatcac
     2701 taatgactaa ttttagtata tagtaaatat agacactaat attaaataaa aaataaaata
     2761 aacatcaaaa attgttctat aaatataaat aattaaaata attaaaataa ttaaaataat
     2821 acaaagaaaa ttatttatta taataataat aagtaacaat tagattatat tgaagaataa
     2881 gaatattatt tcttgattat tatatttata ttatctttct ttaattgtat aaagaattaa
     2941 gtgttatata aagtaataat attatttttc aattaataat caattggcaa ctattaaacg
     3001 gtttattcat ataatagtct taaaattagc aataactatg tacagaactt gcttatctaa
     3061 tttggtaagt tcactaaaca atgagtttaa cctaacaaca gatcctttag tataaaataa
     3121 gctttttcta taggatatag tatgtctata tacccctttg gtatatctat cgataacgtt
     3181 aattaattcc ttttatgtta ttattatagc ccctaaatat catcttgcct tggctctaaa
     3241 gaatactaca atacatcttt taatttttta gagccaagaa aatcaatatt tccattctgt
     3301 agacactata atcttttccc tattttgtca ttgtaatatt tgaaactcta tacattgcat
     3361 aattacaaaa ttattttcat gacgtaaaca ggatcgtact cctaatttaa tatttattat
     3421 tgtttattat atagataatc aggatctata atgattataa gcttaaacga tttcctatat
     3481 agttcctgta aataatcttt tattaatgtc atcttgataa ttgattaatg tataaagcac
     3541 tattttttat tttattattt ttcatttttc actatttgtt tatttcaact acaaaaaatg
     3601 agacaataag tatttttgca caaaatatga ccaatcaaac agaatatttg aatactacag
     3661 aatcatttct tacaaagaca aaaccctacc tccaggttat atcccctagt aatagtacag
     3721 taaaatcttt aataactttt aatgatatat tacccaatgg ttatatattt cctcgcctac
     3781 cagatggttt agcatcaata cataatagag atggacttat tgatattttt gtaaatcatg
     3841 aattagcttt agataaagta aatgaatatg caaaagtatc caagctaact gttgatgaaa
     3901 atggtaggat aatctctgga gaattaatag aagatggatc aggaaagtat gagcgttttt
     3961 gttccgctac aatttttatg gaaaacggat ttaagaatcc tcttttcgta acaaatgaag
     4021 aaatagaaga aggaatcgtt gtcgcttatg atactgtgac aaaaaataaa acggaaatgc
     4081 catggcttgg gaagttttcc catgaaaata cgatattggt ccctaacaaa aacaataaaa
     4141 ctattgtgtt aactacggaa gatggtgacc ccaatcactc tcaattatat atgtttatat
     4201 cagataatcc aactaatttt ctaaatggca ttgggaaatt atatgtcctc attggagaga
     4261 ataatataac tagcttccaa gacttacaga agggcattat atacaatgga catttccaac
     4321 cagtaacctg gaattggatg actcaaaatt ctacggacct tgaaaaagaa gttcagaatc
     4381 taaatgcttt ggactttatg aggttagagg acacagatta taataaagtt aacaattcag
     4441 taatttacat aactgatacg ggatctgacg aatttggtga aagatataag gatgggagat
     4501 tataccaatt tgaaattata gaaccttcgc ttaatcaagc gaataatact aatgttacta
     4561 ataataataa ttattacaaa gtgaaaattt caatactctt agatggaggt gatggaggcg
     4621 acgatctgag aaatccagat aatattgcaa ctagtaaaaa aagtattatg atacaggaag
     4681 accttaatga ttacaatata atagatggag gagttaatgc aagagtttta aaatatgatc
     4741 ttactagcca taatctggat ccagtagcaa tagttgatca atcaccggat gtttctcatc
     4801 caaaagctgg agaatgggag tccagcggaa taatagaagt ttttgatata tttggaaaag
     4861 gttattggtt gttggatatt caagctcata cactggggga aggcggtcaa ttgttattga
     4921 tgaatagtcc tggatcttga gactataatt aaatatattt aacttttgca aacctagttt
     4981 gtttcgtaga tcgtctaact aatcttttag tattattggt tgttgcagta gtataaacaa
     5041 gactataatt gaggtattat taccattttt acctcctata ttcggaaaaa cattctttaa
     5101 ttcattaatg ttaattgtat aaatgcagac aaacttcata gacagactta tagtccaacc
     5161 aaataccaag attcgtctaa gtgattggaa ttcggcttat ttaggaagaa tagataagga
     5221 aacagccgag agactgctaa accaaagcct aatgaatcag atgtctaatc t
//
LOCUS       DASVEV010000301         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_167606, whole genome shotgun sequence.
ACCESSION   DASVEV010000301 DASVEV010000000
VERSION     DASVEV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167606"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF222_13075"
     CDS             complement(<1..399)
                     /locus_tag="VF222_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143401.1"
                     /translation="MREVTQYTLLVAILAILIYIAVVSTGSIATTNANKNSLSELQDS
                     NLASYELVSGNLDYVHTADMNRATQTVLLNGITLNSSEFIILYDSTPYASKGHIALNL
                     PCDPKDPQDRLLDVLIGRAPDMIPMALGYIP"
     gene            complement(862..1347)
                     /locus_tag="VF222_13080"
     CDS             complement(862..1347)
                     /locus_tag="VF222_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143402.1"
                     /translation="MRIWKNVFITITKLFGAEKFYPIVIAIFHLSNSKNNDKKPFIIL
                     NCLCYMRTLRLLNLHCNKTDDDDNTKVDETFLTVDNGKFQGFGPESMSNGSDWNINAN
                     IQFNVEAIVSFFDEDLIRKLSNTKHFRNHIVEENEVSKGPKKVSFSSNNANYVLTYEI
                     I"
     gene            1548..2279
                     /locus_tag="VF222_13085"
     CDS             1548..2279
                     /locus_tag="VF222_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143403.1"
                     /translation="MRILYSNKIISTTILIALLIITGFIIIQPLQVQGQKEIATNIPD
                     PIKVTKCQVVNPPTDPIDMNTVIFKDIAKTIHVEKEIFKCITKNGAPVVAMVSLFTEL
                     FENLRTQSTLNKTVETVTCVKGYNGTVSYCNSNQIPISREYPFAVSCDPLNLRILNIA
                     SPIEMETVVSSNGISKTVEAEKEAFLCTYKQNKPTQILDVMLFTEIIEKVSSGTVIKK
                     NVESISCLKDIGTAKVLKCYTKKHM"
     gene            complement(3305..3634)
                     /locus_tag="VF222_13090"
     CDS             complement(3305..3634)
                     /locus_tag="VF222_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143404.1"
                     /translation="MTHSSKLGDKNANNIFVGDIGVLTDGYRYYFEVNEDRTGLKFDS
                     NSSSSQTGLNDLVADNEQEMSVIALGTDFGGITDIETGPDGFLYILTLDRESDGEDKI
                     YRITQLL"
     gene            complement(3606..4211)
                     /locus_tag="VF222_13095"
     CDS             complement(3606..4211)
                     /locus_tag="VF222_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143405.1"
                     /translation="MNGHNIAVYIIIPLALFSIISLFDNVQAQTNSTSSANQTASQQQ
                     QQNQNANLTAQTLMKTDIVALKNTLMNAQLAIVDGNIKQALKDVMDLETQLILLEPAP
                     PTKFINSIHKAIAAISTSEIDKSLNTLTNIQVSILKAENLIFKAAVANPQVMQQFDTL
                     NYVVPTPQPEKIEQVDISKNVVPTPQPEKIQQFDTFIKTWR"
BASE COUNT         1642 a          636 c          642 g         1611 t
ORIGIN      
        1 tggaatatat cccagagcca ttggaatcat atcaggagct cttcctataa gaacatctaa
       61 aagacggtct tggggatctt taggatcaca tggtaaattt aatgctatat gtcctttact
      121 tgcataaggt gttgaatcat aaagtataat aaactcacta gaattgagag ttattccatt
      181 taaaagtact gtctgagtgg ctctattcat gtctgctgta tgaacataat ctaaattacc
      241 tgatactaat tcataagaag ctaaattaga atcttgtaat tcagatagcg aatttttatt
      301 tgcgttagtt gttgctattg atccagtact aactacagca atatagataa gaatagctaa
      361 aattgcaacc agcagggtat attgagtaac ctctctcata tttgaaaaat actttataac
      421 catcagtatt aaacaatttg gtgaaaggat agatataata aatttgtagt agtagtagga
      481 gtaattatta tagttctatt atttttttct tctctcacct tctttccatt ctttaaaata
      541 ctattatatt aacaaccaat catctggaaa agatttcaat cattatcaga gatcactatt
      601 tggctcaaat atacaagagc tcatagcccc aatcaacaga aaagaccttg aggcagatca
      661 tgcaaaacaa agagatttgc tagttggata aaataataaa ttaacagaaa acagaaaagg
      721 ctttttacat atattctata aagcagttgt aaataaatat tcaatctcta ttaataaaga
      781 tacaaagttt aatattatta caaaatttta aacaaataaa cgttttcgaa ttcaatataa
      841 tatctataga attctttgtt tttatattat ttcataggtt aaaacgtaat tcgcattatt
      901 cgatgagaaa ctaacctttt ttggtccttt actaacctca ttttcctcaa caatgtgatt
      961 cctaaaatgt ttggtattac ttagtttacg tatcaaatct tcatcaaaga aacttactat
     1021 agcctccaca ttgaattgaa tatttgcatt gatgttccaa tcacttccat tgctcataga
     1081 ctctggtcca aatccttgaa acttaccgtt atcaacagta agaaaagtct catctacttt
     1141 tgtattatca tcatcatcag ttttattaca atgaagattt aataatcgta aggttctcat
     1201 ataacataaa caatttaaaa ttataaaagg ttttttatca ttatttttgc tattagaaag
     1261 atgaaatata gcaataacta tcggataaaa tttttcggcg ccgaaaagtt ttgttatagt
     1321 tatgaaaaca ttcttccaaa ttctcaaaaa ctataatcat atttatcaat atcaattttt
     1381 ttaataatga caatactagt tccattgcat ttttctcaat tgtctattag gtcataacat
     1441 cgaattgact aatgaacaaa tagaataaaa aacagtaaaa atttattaaa aaaattagga
     1501 taaatgatgc atagctttga tattttagtt aaagtaaaca ataattaatg agaatactat
     1561 actcaaacaa aatcatctct acaactattc taattgcctt attaataata acaggtttta
     1621 taattataca accattacag gtacaaggtc aaaaggaaat agccacgaac attcctgacc
     1681 caataaaagt gactaaatgc caagtagtta atcctccaac tgaccctata gatatgaaca
     1741 ctgttatttt caaggacatt gcaaaaacta ttcatgttga aaaagaaatt ttcaaatgta
     1801 taacaaaaaa tggtgctcca gtagtagcaa tggtaagtct ttttacagag ctatttgaaa
     1861 acttgagaac tcaatctact ttgaataaaa ctgttgaaac tgtcacatgt gtcaaaggat
     1921 ataatggtac agtgtcctat tgtaactcaa atcaaattcc tatttccaga gaataccctt
     1981 ttgcagtaag ttgtgaccct ctgaatttaa gaatattaaa tatcgcatct ccaatagaaa
     2041 tggagaccgt agtaagttct aatggcattt caaagacggt ggaagcagaa aaagaagcat
     2101 ttttgtgtac ttataaacaa aataaaccta ctcaaatatt agatgtgatg ttatttacag
     2161 aaataattga aaaagtttca agtgggacag taattaaaaa gaatgttgaa tcaatttctt
     2221 gcttgaaaga tataggtact gcaaaggttt taaaatgcta tactaaaaaa catatgtaaa
     2281 ttcaaatcac ctaataatat tttttcttga taaatattag gtaataattt ttattaattc
     2341 actctttaga tttagagcta tagaagaaac tatttgcatt tagtagtaaa atgtattgtt
     2401 attttgaatt ttcagcataa atgctatttt taagcgccaa aaaaaaataa atttaaaaaa
     2461 gatagatttt caatatctta ataggaagta aaacagctta tgttacatct atatagatat
     2521 ttcaaaactt tttgttttga aatgctgttt gtttgagtgt acatagattg ttgggtttgg
     2581 cgtcttcttc taacaatgac tacaaactaa ccccataaaa ggaagagcac cgattatggt
     2641 tattcctaca atcattccta ctatgcagat actaaatttt gttaacttga acatatatta
     2701 ttattctttt tagaatagaa aaagattttc cacatcataa atagcgttac ctgtcaaaag
     2761 gaaacataat gttgaactta caaaaagaag actaatcata ttgtaaggta gaccctaaga
     2821 acgatagaga ttcagtaata agactctaat ataagaaaac acagataaat atttaaattt
     2881 ttttatctat cataaaaata atttgtaaat taatattaag attcaagacc tagaaacaat
     2941 taaacccctt ttttctggag tagagccttc catgtagtgg ggttctatta cattgtatat
     3001 agcttttatt cttagaaact tattttaaaa ttgtttttgt attatcagaa ggtatagaaa
     3061 cttgaaatat gccaaggagg gaataatatc gatagccgtg caaaaataag agtctattag
     3121 agatatattg ctaacttcta ttttgctttt attattagaa cttatttttt aaaatatatt
     3181 tggaatatta agatgtaaat tctttagaaa tttgaaggag ggagtaatac cgatagcggt
     3241 tctacaatag aagtcaaatg tagtaactat tattaatttg tatttacttg ttggtaacgt
     3301 attctcataa tagctgtgta attctataga tcttatcttc accatctgat tctctgtcta
     3361 atgttaatat atacaaaaat ccatctgggc cagtttcaat atctgttatt ccaccaaaat
     3421 cggttccaag agctattaca gacatttctt gttcattgtc agcaactaag tcattcagtc
     3481 cagtttgact actactacta ttactatcaa attttaagcc tgttctatct tcattaactt
     3541 caaaataata tcgatatcca tcagtcagga cgccaatatc tccaacaaaa atgttgttgg
     3601 cattcttatc tccaagtttt gatgaatgtg tcaaattgtt gtatcttttc aggttgagga
     3661 gttggtacta catttttcga aatgtcaact tgttctatct tttcaggttg aggagttggt
     3721 actacatagt tcaaagtgtc aaattgttgc atcacttgag gatttgccac tgcagctttg
     3781 aatattagat tttctgcttt taatatacta acttgaatgt tagtaagagt attcaatgat
     3841 ttatcaattt ctgatgtaga tatagcagct atagctttat gtatactatt tataaattta
     3901 gtaggaggag ctggttctag taatatcaat tgtgtttcta aatccataac atcttttaat
     3961 gcttgtttga tattaccatc aactattgcc aactgtgcgt tcatcaaagt attctttaat
     4021 gcaactatat ctgttttcat taaagtctgc gcagtgagat ttgcgttttg attttgctgt
     4081 tgttgttgag atgccgtttg attggcagat gatgttgagt tagtttgagc ttgtacgtta
     4141 tcaaacaatg atataataga gaagagagca agtggaatta ttatatagac ggcgatatta
     4201 tgaccattca tgtaaatagc atactagaga ttatttatat gacttgtgaa taaaatctca
     4261 tatagaataa cacataaaat tctctaaaag tttctaataa tactgtttta aattatttta
     4321 tatattaaca atgtaaatta taatagataa aaaaaccaat atcataattt atactatcag
     4381 tataacaaac gattgattct gtggatattc ttatatcaaa tgtaagaatg tatttagctt
     4441 ttattgttag aaacttattt taaaataaag ataattgtaa aatattagtg tataatttca
     4501 tacaagttct gtggaatggg attgaaacaa t
//
LOCUS       DASVEV010000302         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1138386, whole genome shotgun sequence.
ACCESSION   DASVEV010000302 DASVEV010000000
VERSION     DASVEV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138386"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..204
                     /locus_tag="VF222_13100"
     CDS             64..204
                     /locus_tag="VF222_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143406.1"
                     /translation="MQICGYVQGALYQTSNNYVEDDGDLTRDGETALVVQEMELYLEG
                     DL"
     gene            complement(411..1076)
                     /locus_tag="VF222_13105"
     CDS             complement(411..1076)
                     /locus_tag="VF222_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7143407.1"
                     /translation="MSFGFGDNKEDKDTEHTKKPLLDGDDLDEVNKIKDMLNPDEKVL
                     LVAKQSKIKPGGSYFTPNIIYATDRRIIIRDPYMLGLKANVVDIPYDIITSLKLEEGL
                     LSSTIRFKAPGLMSSTRLGMMDKIIEGEDDETGIIEAIPKDKAEHLIEIIRSGMQGSR
                     RAAASKKQKSIELDEPKEDTNIPNQSVSIADELEKLAKLKEEGLISKEEFNQMKQDLI
                     KNK"
     gene            complement(1229..1600)
                     /locus_tag="VF222_13110"
     CDS             complement(1229..1600)
                     /locus_tag="VF222_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143408.1"
                     /translation="MVAKLSTTINKIQSLPNFSNSKIINEFLRYMKNNGSSERHQNNN
                     LNVLIEFSNYFDSYNTFYDINKKEQILSFLDTKIKDSEKDPEKRWITSWNHYLTRIKL
                     FFRFIIATVNTENNKLKGRIP"
     gene            1796..1939
                     /locus_tag="VF222_13115"
     CDS             1796..1939
                     /locus_tag="VF222_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143409.1"
                     /translation="MSKQYTKTLSVTSSGGLINVTNHPPPAGEVIGLSSSINWAGSIA
                     PLH"
BASE COUNT          808 a          382 c          372 g          984 t
ORIGIN      
        1 atattagggg cttttagtat tactcagggt tatggtacat cctctaattg aaataccgat
       61 tggatgcaaa tatgtggtta cgttcaaggg gctctttatc aaacatctaa taattatgtt
      121 gaggacgatg gagacttaac aagagatgga gaaactgctt tggttgtaca agaaatggag
      181 ctttatttgg agggggatct ttaacattgg gacttccacc tggaataatc actcgaggat
      241 tagaaatgtt agctggtcct acaggctgcg acgggattgt taattttcaa attgaattaa
      301 tattggtggt ttaggtacaa tattaaacct cattggctaa aagacttttg aatgaagtat
      361 tgcctatcat ttttagccaa taaacttcag aataagaaat tctactatct tcacttattc
      421 ttaataagat cttgtttcat ctgattaaac tcttctttac ttattaatcc ttcttctttt
      481 agttttgcaa gtttctctaa ttcatctgca attgatactg attgattggg gatatttgtg
      541 tcttctttag gttcatctaa ttcgatagat ttttgctttt ttgatgctgc tgctcttcga
      601 ctaccttgca tacctgaacg aatgatttca attaaatgtt ctgctttatc tttggggata
      661 gcttctatta tacctgtttc atcatcttca ccttcgatta ttttatccat catccctaat
      721 ctggttgagc tcattaatcc aggagcttta aatcttattg tagatgatag caatccctct
      781 tcaagtttca aacttgtaat tatatcgtag gggatatcaa ctacatttgc ttttaatcca
      841 agcatatatg gatccctaat tatgattctt ctgtctgttg catagatgat atttggtgta
      901 aaataagaac ctccaggttt tatttttgat tgttttgcaa caagtaatac tttttcgtct
      961 ggatttaaca tatcctttat tttattaact tcatctaagt catctccatc aagaagaggt
     1021 tttttagtat gttcagtatc tttatcttct ttattatcac cgaatccaaa agacatatta
     1081 tgcgatctaa cttagaacgt ctcatataaa atacattatg tttctgtata cctttaataa
     1141 ccttgtctag atttttttta attatatgcc ccacgatata aatattttaa atctgttatt
     1201 gataaaaatg tgtagaatct gaaaaaaact agggtattcg gcctttcagt ttattatttt
     1261 ctgtatttac tgttgctata ataaatctaa aaaataattt tattcttgtt aaataatggt
     1321 tccaactagt tatccatctt ttttctgggt ctttttctga gtcttttatt ttagtatcta
     1381 aaaaagatag tatttgttct ttcttattga tatcataaaa agtgttatag gaatcaaagt
     1441 agttggaaaa ttcaataagt acattaagat tattattctg atgcctttcc gaagaaccgt
     1501 tattcttcat atatcttaga aattcgttaa ttattttgct attagaaaaa tttggtaaac
     1561 tctgaatttt attaatagtt gtagataatt tggctaccat cttggactaa tacctcttct
     1621 tataaagttg attttcattc tcaattatct gaacccatgt atataacggg ctcagtggga
     1681 gttgaaccct tctataaagt gggaccagat actttctaaa tttatctatt tcttaaaaat
     1741 tttgcttttc aattagtatt aaataaaaaa taaaaaagtg gattgttatg gcgaattgtc
     1801 aaagcaatat acaaaaacgt tgagtgtaac gtcgtcagga ggacttatta atgttaccaa
     1861 ccatcctcct cctgctggag aagttatagg cttatcatcg agtattaact gggctggatc
     1921 aatagcgcca ttacattagc ttaatgaatg gttattatga tttatagttg ctaattttta
     1981 ttagttaaat caattgttgt cctatatatc atttgaccat tgtatgaaaa tagatttatt
     2041 attacaaagc aaataattta atttagatag aaaatcaatt tattatttat ttatttttta
     2101 aaagcagtaa tattgatgat aggtaatcct agattaatca aaacaaaaat aaaataaaaa
     2161 aattaagcag tcctaaagtc gaataaaact ttaatcggac tttctactgg tttactgtta
     2221 attgatatta tttcgacact tatcggatat gtagaaccct tttttaaatc tgactggaca
     2281 tggtaagctc cagtactact aaaatctggt tgattctgga actattcaac caaaaggatt
     2341 catagaatat gtagcaacta acttatctcc aggaaattat actttctaca caactacgga
     2401 cccaacagta actgcacaaa tctcaatact acaaaaataa caaatttatt ttagttattt
     2461 taattttttt taaaatatat aataccaagt aaatcttaaa tattatcttc tttattacca
     2521 tatagtagac tcttttttta gaagcg
//
LOCUS       DASVEV010000303        18533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1139122, whole genome shotgun sequence.
ACCESSION   DASVEV010000303 DASVEV010000000
VERSION     DASVEV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 18533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18533
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139122"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1255)
                     /locus_tag="VF222_13120"
     CDS             complement(323..1255)
                     /locus_tag="VF222_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7143410.1"
                     /translation="MFKPNDTIVSKTASGILLIFGISILIIGVGATLYTLNNNFVIFL
                     SAIGVILSIFSFIYIIRQYERSIILRLGKYHKRIGPGINYRIPFVDNVLVIDIRERVR
                     EFKAERMLTKDNVPVTIDAILRYKIIEDRAKDAILNVENFNEMIQQVSQTTLRNNIGS
                     SLFQDILSKREEINQHIRSIISNEASNWGIEVVGVEIRQVIIPQELEAAMSKQAQAER
                     EKNARIIYSESEVLVANKFNEASKVYTNNPVAYALRQSNMLFESIKVQGNTIVMVPSE
                     TLNSMGFGNLAMTVAYLENTKKAAATMEQKENKN"
     gene            complement(1750..2253)
                     /locus_tag="VF222_13125"
     CDS             complement(1750..2253)
                     /locus_tag="VF222_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143411.1"
                     /translation="MSEITSLFISIVILFFPDLLNKKYVFSYFWDVLSKSYKTDAEPV
                     KDFFIDIDSNKFDKVSKKETITKYFGYGFSVIGLFITIIITSFSIIAIILSNVGNFSL
                     ITVNLIIFIILIILVFEIQHLISHNKADKEKLHVYNSKLMLKLKIIIVVINLCLTLFS
                     LSLTNNS"
     gene            complement(3332..5527)
                     /locus_tag="VF222_13130"
     CDS             complement(3332..5527)
                     /locus_tag="VF222_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7143412.1"
                     /translation="MSIAEVRHLPKVIGILLVTEKEFFSDIDPLPFSSSSKLLYSYLE
                     EVVKQHLDVFESLWNKAVSGKKKIAEIEKGIVSKITEERIRQIEEEKDQIETRIIETP
                     TQFHNFMVSSLKDTIERDWYFQDEILRSIYENYFELFKENMKKTLLLKRMNQNRKSKS
                     SLNKKDNIIGLRCILKITRQNMELVGKFLEVEGVYIRHTSNPLPINFEVSDTKFNLSM
                     RTSNKIDTYVLSIPDSSLIKHFKSTFEDLWNEGLDAKIKINDIRTNLNVEEGFTELIE
                     NPYKTQQLLIHHLQKATKEILIILPSINAFFRYRKIGIFDILEKKLLHNNHNSYRENS
                     NKLLVKILTPTNNKLEKNITSIYNKIKDTKKQKENIEFRRIESLSEINTACIIIDKKD
                     LLVIELKDDSKDNFLEAIGSSIYSNTPTTVFSYISIFDTLWTQTELYESIRTANDELQ
                     STTEELRESLQILANVNQELTLTNEILNKHGRMQKEFINVAAHELRTPTQSIVGYCEM
                     IDAFPENTQKYLEPVKRNAEKLYRLTEDILDVSRIESGTLKLEKSKFDLREIIVNLIE
                     DLTAKMKKEEDIIQSNKANNDKNTNIQLIYPQLSAEPLFVYADKNRIQQVLSNLLSNA
                     LKFTEDGVITINTEKFMDTVNINSNENNKEKEFVSIKIKDTGKGIDPEVLPRLFEKFA
                     TKSDKGTGLGLYISKNIIDAHGGKIWGEDNNKYGKNGAEFGFTLPLYNI"
     gene            complement(6038..6838)
                     /locus_tag="VF222_13135"
     CDS             complement(6038..6838)
                     /locus_tag="VF222_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143413.1"
                     /translation="MILYIFLSHDVDWRKQGPEKEHILARKDRFENSVFERVNQENLY
                     YNFLEIMDLEEKFGVRSTFFFRTHYENGNYLDYEEEITSLMDGNWEIGLHTDGSSIKN
                     LDWLQKEKTNLEILTRKPIFGNRVHYLNFDNKLLSKLYQIGFTYDSTMKKYKNSISID
                     DMNYRKIDGILEFPITIMDTYLFSYLGVTEENILNVFEETISTATCKNESDNIKIITL
                     LWHDNVLKMKGGRMYKNILEYLTSKNNIKIMRGIDLVNIINKFDKGLT"
     gene            complement(6856..7521)
                     /locus_tag="VF222_13140"
     CDS             complement(6856..7521)
                     /locus_tag="VF222_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX7143414.1"
                     /translation="MRKVIIFGTGNFAQIIYIYLKQSPEFEVVAFTVNEWALKDNKIF
                     CSLPVVPFETVQDVYPCTKFDMFIAVGYTNMNKKRAKIFDEAKTKGYKLISYVHPTTS
                     ISEEFHMGENCFIFEKNVIQPFVTLGNNIIIWTGNVISHHTTIKDHCFIVSHSAIAGN
                     VTINSFCFIGMNATIRNGIKIAKETVIGAGAIILNDTEEKGVYANKSTIKLDITSDKL
                     KSL"
     gene            complement(7971..8330)
                     /locus_tag="VF222_13145"
     CDS             complement(7971..8330)
                     /locus_tag="VF222_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143415.1"
                     /translation="MISTILLIITFATTISIIGASSPIINTFAQNDDASNPMTSDTSN
                     NMSMTNENMDASSDNLTGGNEQQGSEGTLDKIKDKLGGILGNGGNDQSTTGENSVQNS
                     EGTLDKIKDKLGGILGN"
     gene            8581..9207
                     /locus_tag="VF222_13150"
     CDS             8581..9207
                     /locus_tag="VF222_13150"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX7143416.1"
                     /translation="MSSGSFTPFIKVEHVISFSDAVFAFAITLMALSIDIPDLPSDLT
                     QAELVDKLNGLFPQFESYIISFAVIAIFWVSYHQVFNHIKGSHIIMVYLNLLFLLLIT
                     LLSLSTSLVINYGGYHIPYIIYSFMVILTSSLLAMIWWHATKDKLLIDKNLHSLYIKG
                     VMINLISIPLVFTISIIISFVNLDIAEYFWLVIAPLNIAIKQKYKHSK"
     gene            10013..10561
                     /locus_tag="VF222_13155"
     CDS             10013..10561
                     /locus_tag="VF222_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7143417.1"
                     /translation="MKTILTTTTFLIALLAIGLIGINNNNNSIGNVFAQVDPEDSPMM
                     EDMEMDEGMMTMSNQSEGMKMMNWTGTIDVKTTIGEAFKSKVTVNILDAINAAQTNVG
                     ANSFVKKAELTPAHGYLVYKIMVVDENMKKYKVIVDPGNGQVLMKKEVTWYEDDEHKM
                     KYGEEKGDKYGHDYKMMKDKEY"
     gene            11705..11902
                     /locus_tag="VF222_13160"
     CDS             11705..11902
                     /locus_tag="VF222_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143418.1"
                     /translation="MPMLMLKCKTCGEVFPGIYIVEESTNDDEFKSSITKNNMIHSCS
                     RGHQNEYSIEDYMDWSKIEII"
     gene            11889..13019
                     /gene="hflX"
                     /locus_tag="VF222_13165"
     CDS             11889..13019
                     /gene="hflX"
                     /locus_tag="VF222_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7143419.1"
                     /translation="MKSSKVILVTYPDDFIINEGKELILSIPEYEIVKVFTQKYLNHS
                     KYGMGSGKAEEIEKFVKETNEIEEIIVDEHLSSKQIYNLEKLIGIPVKDRERLILDIF
                     YTRATTVEAKLQIQLAEIEYEMPRVRENAKLLSKSNERAGKGGMGEYIVDVQFRDLKR
                     QMSFIKEKINDAKIKRNIYHHQRQKTGIPVVSLVGYTSSGKTTLFNLLTNEYKETSSN
                     LFTTLSTTTRSFKVPHNNKNKEGEGGVLIVDTVGFISRLPTYMIDAFKSTLEESLGAD
                     LILLLLDSSEKIEDMRIKYSNCLDILEELNVDKSKLFTVLTKVDKIDTRMINEIVEKL
                     EISSDVIAISSNTGYGIHKLKTLLMRRQFLENKYNENTNVKR"
     gene            13237..13857
                     /locus_tag="VF222_13170"
     CDS             13237..13857
                     /locus_tag="VF222_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7143420.1"
                     /translation="MEICNIDAIQYLCKVDSNLEKIIKIVGKYSIKIRNDPFQSLVES
                     IIYQQLAGRAANAIYNRFINYYGNKQVTPIGILNSPNDNLKKVGLSNRKIDYLKDLAS
                     HIYDGRINLEELSKMNDEEIINKLINVKGIGRWTSEMFLIFSLGRQDVLPITDLGVRK
                     AMHKVYSLSELPKPNTMMEIAEPWRPYRSIATWYLWKSLSNFSTIG"
     gene            14726..16585
                     /locus_tag="VF222_13175"
     CDS             14726..16585
                     /locus_tag="VF222_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7143421.1"
                     /translation="MNLNIFTIIISFFALLSVSLVYGEENINNNNSLQQNSNNTATNN
                     KILDYKSEPFLLLDGEDFEDIAHNNTLSLENFAIYTWIKTNQTNLVDPAHIVNKGGFN
                     TDEKGENMNYGIWFSTDGTISGGFETESGENFEVKSTAKYNNGKWHYVLLSYDGSLLR
                     LDIDGEKQLSTTKNTNGAIPDTTGEQPIRIGANSLDESKFFTGNIDEVRVWNRGLTDK
                     EISEIYMNNAFDSNGLVVHLNFGGYSTPTTNNNSSSLLSSSTINHTTLKENLTSTTTN
                     KESIPDTINQTTSSSSLPGSIAKINATDSHRLQDNKIMTTTTSSSFNIAVSADWGCTE
                     DTKKTAENIQKKNPEIVIAAGDLSYEESANCWFEIIQPFKSKMKIAMGDHEYSDTSGG
                     TIGVIYQYLKPLNLTKTYYSFDMNKVHFVIIDPYIDYNAGSSQYHFIEQDLKIASTNP
                     KIDWTFVVESIPIYTSPAQHPANSTIRDIYHPLFDKYGVDLVFTSDNHNYQRTFPLKY
                     NSKGGDSSNNPIIVDSKQNNYYNNDKGGVIYLITGTAGRSLYEIKQQAPFVAKQSDEH
                     FGFLNIDIYDKMLKGTFYANERELPHHYYVNFQNNIIIDHFTISKTNSPNNNL"
     gene            complement(17191..17325)
                     /locus_tag="VF222_13180"
     CDS             complement(17191..17325)
                     /locus_tag="VF222_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143422.1"
                     /translation="MGCELFTIGIVAGLTLTAAALVFQFFSDRRKEFQNLTNELEVNN
                     "
     gene            17841..18017
                     /locus_tag="VF222_13185"
     CDS             17841..18017
                     /locus_tag="VF222_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143423.1"
                     /translation="MRDNKKITNKNRIIDDHSNWRTSSRVADYLNRVDKIPHRAEGEL
                     LLCDFIPKKNKKNS"
     gene            17980..18516
                     /locus_tag="VF222_13190"
     CDS             17980..18516
                     /locus_tag="VF222_13190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7143424.1"
                     /translation="MISYQKRTRRILDLGTGNGRLIKLLKEKIPTVKSVTIDFSPPML
                     NEFKREFLNDDTIKIIEHDLTYPLPTNLGKFDAIVSNFAIHHLMHNRKKDLYSEIFVI
                     LKPGGIFCNLDHISSESKKINQYFRKVMGRRPINKEHGKRLTKIHIQLEWLLEIGFVD
                     VDCYWRWLEFALLIGFKP"
BASE COUNT         6619 a         2522 c         2445 g         6947 t
ORIGIN      
        1 tataagtaat tcaaattgta tttttgattc aggaatttta tttacctcct ttttttctat
       61 tataacacca atatcagata accattgaat tatattgtta aaagcaactt cttttacatc
      121 tagttcgtta ttcatcttta actataaaat tgatgaaacc cattgttttt attgttgatc
      181 acttactaaa tgaaaaaaaa tagaatggtt taatgaacaa caatattttt aatagcaaat
      241 tatttcaggt gttatgtgga tttttattct ttgaacattt gttacatcta aaatgcttat
      301 ttgaaaattt ttttatcatc tatcaatttt tattttcttt ttgttccata gtagctgctg
      361 cttttttagt attttcaaga tatgctacgg tcatggccaa attgccaaaa cccatggaat
      421 tcaaagtttc agaaggaacc attactattg tattaccttg aacttttata gattcaaaca
      481 acatgtttga ttgtcttaat gcatatgcaa caggattatt tgtatatact tttgatgctt
      541 cattaaattt atttgctaca agaacttccg attcactgta tataattcta gcattttttt
      601 ctcgttctgc ttgggcctgt tttgacatag ccgcttctaa ctcttgtggt ataattacct
      661 gacgaatttc aacacctaca acttctattc cccaattact cgcttcatta gatataattg
      721 atctaatgtg ttggtttatt tcttctcttt tagataaaat atcctgaaat aaagatgacc
      781 caatattatt tcttaatgtt gtctgtgaaa cttgttgaat catttcatta aagttttcta
      841 catttaaaat agcatctttt gctctatcct caattatctt atagcgcaaa atcgcatcaa
      901 ttgtaactgg aacgttatct tttgttaaca ttctttcggc tttaaattct ctcacccttt
      961 ctctgatatc aattaccaag acattatcga caaacgggat cctatagtta ataccaggac
     1021 ctattcgttt atgatatttt ccaagtcgca atataataga cctttcatat tgtcttatta
     1081 tgtaaatgaa tgaaaaaata gatagtatta ctccaatcgc tgataaaaaa attacaaaat
     1141 tattattcaa tgtataaagt gtagcaccaa ctccaataat caatattgaa attccaaaaa
     1201 ttagtaaaat tccagaggcc gttttgctta caattgtatc atttggttta aacatagatt
     1261 ttgcatcgga cataaatatt atatgtattg caatgattta ggacttactg ctattttgtt
     1321 ttttaattgt tattgggtat ccaatttgca aatacgaaga aaaattacac ttatttaatt
     1381 aataaatttt caatgagaag taaagaatta caagtgttaa tttgatagta actataacag
     1441 tggctttttt atttctttca aatctatatt cataaaataa atagatattc ctagctaaag
     1501 tttttactac catctaagaa gaattatttt attaacgaat tgaatgaaag aagtaaatat
     1561 atcttaaatt aatagaacgg attggcacat ttgaaaactt ctttctcgtt gtacaatcaa
     1621 aaattcatgt taagaacaaa attgaaagga atacaaataa aaaaatatat aattggattg
     1681 acatcatcat acaaaacaaa aatctaagct ttaaattttg agttttaaat cttttatttt
     1741 atatttaatt tatgaattat tcgttaatga aagactaaag agagtaagac acaaattaat
     1801 tactacaatt attattttta gtttaagcat taattttgaa ttatacacat gtaatttttc
     1861 tttatctgct ttattgtgag aaataagatg ttgaatctca aataccaata ttattaaaat
     1921 aataaatatt attaaattaa cagtaataag agaaaaatta ccaacatttg ataatattat
     1981 ggctataatt gaaaatgaag taattattat tgtaataaac aaaccaataa cagaaaaccc
     2041 gtatccaaaa tatttagtta ttgtctcttt tttagatact ttatcaaatt tatttgaatc
     2101 tatatctata aaaaaatcct taacaggttc agcatcagtt ttataagatt ttgataatac
     2161 atcccagaag taactaaata catatttttt gttgagaaga tcagggaaaa acagtattac
     2221 aattgaaata aaaagtgaag ttatttctga catatcaatt taattctata attacaattt
     2281 tcgttatgag gcagttgtac ctctttagta gtattacctt taaaaacata ataactggaa
     2341 atattttctg gaatattata taacatatat ttttttctct gttaaacaaa ctagaatgat
     2401 acgttttatt ttcatctttt aaaattgttt ttttatctac tactaaataa ttattcttat
     2461 tctgaaatat gcaattttgg tttgatacac tactacaagt aatagtttct agcaggagaa
     2521 tgcttgatgc attaatagtt tcaattttat gtttagtctt atctgtaatg tcgaacatat
     2581 cagttgagaa attgaggtcc acaaaaatat tctgtctact tttacattta ttcttattca
     2641 tttcattctt aaaattaaac gagtttaata tagatataaa ttaataagta gtttaatctc
     2701 aatgatctaa ttttttaata gttttttttc ttttatccta aacctgaaac taatataagt
     2761 attgtaaaag ttactactag tgaaggaata gaagaagaaa gtgattatcg ctctccattt
     2821 aaaggaatag aattacctgg aagtggaaga taaataatgg tataatttat tactcatcat
     2881 cgtagatttg atagtctttg ttatcatttc tttattcgta atcctaaaat attcaactaa
     2941 gatgatcttg ttttttactg gagtcgtgat tgagaaccta agcacagcat tatcatagaa
     3001 tacattatct tatttaatta aaatcttaag ataagattaa gttttgaaaa ttagtataat
     3061 atagcttcaa taaattagaa gtaacaataa aattttcctt gttatattat agcacaatta
     3121 aaatgttaaa agactaatta gaagttataa aaagagattt tacttcaatg aaatacatca
     3181 acaagaacta ttgatatttc cttcgagcgg atcatgtaaa caagaaattt tttacctaaa
     3241 gaaatcttaa attcacggaa ataaatattt agcattaata aacgatacat aaattataag
     3301 ttaatcaaat tttatatata agaatttaat tttaaatatt atataaaggt aaagtaaaac
     3361 cgaattctgc accattcttt ccatatttat tgttatcctc tccccatatt tttcctccat
     3421 gagcatctat aatatttttt gaaatgtata aacccagacc ggttccttta tcagattttg
     3481 ttgcaaattt ctcaaatagt cgtggcaata cctcgggatc gattcctttt cctgtatctt
     3541 ttattttgat agacacaaat tctttttctt tattattttc attactatta atattaacag
     3601 tatccatgaa tttttctgta ttaattgtaa ttactccatc ttcagtaaac tttaaagcat
     3661 tacttaacaa attagaaagt acttgttgta ttctattttt atccgcataa acaaataacg
     3721 gttctgcaga taattgtgga tatattagct ggatattggt atttttatcg ttgtttgctt
     3781 tgtttgattg tattatatcc tcttcttttt tcatctttgc tgttaaatct tcaattaaat
     3841 ttactattat ttctcttaaa tcaaatttgc ttttttcaag ttttaacgta ccactttcta
     3901 ttcttgatac atccaagatg tcttctgtta atctatacaa tttttcagca tttcttttta
     3961 caggttctaa atatttttga gtgttttcag gaaaagcatc tatcatttca caatatccaa
     4021 caatagattg agtaggtgtt cttaattcat gggctgcgac atttatgaat tctttttgca
     4081 ttctaccatg tttatttaat atttcattgg ttaatgtaag ttcttgattt acatttgcta
     4141 atatttgtag cgattctctc aattcttctg tcgtactctg cagttcatca tttgctgttc
     4201 taatactttc atataattct gtttgggtcc aaagagtatc aaaaattgag atataggaaa
     4261 aaacagtagt tggagtatta gaatatattg atgatccaat tgcttccaaa aaattatctt
     4321 tggaatcatc ttttaattca ataaccaata aatctttttt atctatgatt atacaggcag
     4381 tattgatctc tgataatgat tctattcttc taaattcaat attttctttt tgtttctttg
     4441 tgtcttttat tttattgtaa atacttgtta tatttttctc taatttattg ttagtaggtg
     4501 ttaatatttt gactaataat ttgttactgt tttctcggta actattatga ttattatgta
     4561 ataatttttt ttctaaaata tcaaatatcc ctatttttct atatctaaag aaagcattta
     4621 ttgatggaag gatgattaag atttctttag ttgctttttg taaatggtgg atcaaaagtt
     4681 gttgagtttt ataaggattt tcaattaatt ctgtaaatcc ttcttctaca tttagattag
     4741 ttcttatatc atttattttt attttagcat ccaaaccctc attccatagg tcttcaaatg
     4801 tagatttaaa atgttttata agtgatgaat ctgggatgga aagtacataa gtatctattt
     4861 tattagatgt acgcatagaa agattgaatt ttgtatctga tacttcaaaa ttgatgggta
     4921 gaggattact ggtatgtctt atgtaaacac cttctacctc aagaaatttt cctactaatt
     4981 ccatattttg tcgtgttatt ttcaagatac atcttaaacc aataatgttg tcttttttgt
     5041 ttaaagatga ttttgacttc ctattttgat tcatcctttt taataacaat gtttttttca
     5101 tattctcttt aaataattca aaataatttt cataaatgct tcttaatatt tcatcttgaa
     5161 aataccaatc ccgttcaatg gtatctttca aactacttac cataaaatta tgaaattgtg
     5221 taggagtttc aataattctt gtttctattt gatctttttc ttcttctatt tgccgaattc
     5281 tttcttctgt aatttttgaa acaatacctt tttctatttc tgctattttc ttttttccgg
     5341 aaacagcctt attccataaa ctttcaaata cgtcaagatg ttgttttact acttcttcca
     5401 gataactata caggagttta gaagaactag aaaacggtaa tgggtctata tcactaaaaa
     5461 actccttctc agttactaat agaataccaa tgacttttgg taaatgtcta acttctgcga
     5521 tcgacatcag ctgcctacac tgttctatat tatcttcagt gacttctgta atacatctaa
     5581 gttttacacc tcgactttga gcatgtctgt agctattctg tatttcgaaa ttagatgcat
     5641 aactttgaag aaaagaggga attaaacaag catttacaac gctttccgca ttattaagta
     5701 ttttatttac tttttctaaa atatctcctg aaccttgaat tatttctatt ttattgtgga
     5761 aaaaatactt ctcattcagg attagatcac tttctttaat tgcttgttta attatatata
     5821 tatattgaga tatttgtatt taacaataat tatatttcat gtataacctt ctttaaacac
     5881 attataaata ctatcatcat caatttttat gttatataca aattatattg catttttata
     5941 ttctggaata ctttggtaat atgttgtttt agataaacta ttatattcta aatatgatcc
     6001 tcttaatttg aatttattta cttactgttg attttgttta tgttaaacct ttatcaaatt
     6061 tattgataat attaacaaga tcaatacctc gcataatttt gatattgttt tttgatgtca
     6121 aatattcaag tatattttta tacattctcc ctcctttcat ttttaaaaca ttgtcatgcc
     6181 aaagtagagt tataattttt atattatcag actcattttt gcacgttgcg gtagatatag
     6241 tttcttcaaa aacatttaat atattttcct cagttactcc taaatatgaa aataaatatg
     6301 tatccattat agttattggg aattctagta taccatcaat ttttctataa ttcatatcat
     6361 caatactaat actattctta tattttttca tagttgaatc ataagtaaat ccaatttgat
     6421 agagttttga tagtaattta ttatcaaagt taagataatg aactctattt ccaaagattg
     6481 gctttctagt aagaatttct aaattggttt tctctttttg aagccagtct aaattcttga
     6541 tagaactacc atccgtatgt aatcctatct cccaatttcc atccataaga gatgtaattt
     6601 cttcttcata atctaaataa ttcccatttt cataatgtgt tctgaaaaaa aaggtggacc
     6661 taacaccaaa tttttcttcc aaatccatta tttctaaaaa attataatat aaattttctt
     6721 gattaactct ctcaaatact gaattttcaa atctatcttt cctagccaaa atatgttctt
     6781 tttctggtcc ttgttttctc caatcaacat catgagataa aaaaatatat aatatcatat
     6841 cgatacttaa agaaactata atgattttaa cttatcactg gttatatcta atttaatggt
     6901 agacttattg gcatagactc ccttttcctc ggtatcattt agtataatgg ctccggcccc
     6961 aattacagtc tctttagcga tttttatacc gtttcttata gtagcattca tgcctatgaa
     7021 acaaaatgaa ttgatagtca catttcctgc tattgcggaa tgtgaaacaa taaagcaatg
     7081 atcttttatc gttgtatgat gacttattac gttacctgtc caaattatta tattatttcc
     7141 taaggtaaca aaaggttgaa taacattttt ttcaaagata aagcaattct cacccatatg
     7201 aaattcttca ctaatcgaag tagtagggtg tacataactt attagtttat acccttttgt
     7261 ttttgcttca tcaaatatct ttgctctttt cttattcata ttagtatatc ctactgctat
     7321 aaacatatca aattttgtac atggataaac atcttgaact gtttcaaatg gaactacagg
     7381 taagctacaa aatattttat tgtcttttaa agcccattca ttaactgtaa aagctacaac
     7441 ttcaaattcc ggagactgtt ttaaatagat atagataatt tgtgcaaagt tacctgttcc
     7501 aaaaattata acctttctca attaaatttt ttaaatataa caagtatatg gatttttgtt
     7561 tttattatat ataaagcatc taatagaatg attattcttt ataaagagta ttataaataa
     7621 aaatacgtag ctacttatca tagtttagag attaatttaa aagttatctc tataaacaat
     7681 taacaggata ttacatacgt tggttgatat ttattatatc gtaaacattt ctgaatatat
     7741 tttatcttga tttatatcga ttttatgatc ttcatataaa attcatgtgc atattccatt
     7801 gaatatgtaa aactttatac aatttactga atattaaatt caaaatactt ggttcgtaat
     7861 aactaaattt aattacctat ttttactatg gaagttatag ctttgaataa ttactttaaa
     7921 aaaaagaata tgttactcct ttattagaat tatctatgat ctattatcta ttagttacct
     7981 agtattccac ctaatttgtc ttttatttta tctaaagttc cttctgagtt ttgtacagag
     8041 ttctctccag ttgttgactg atcatttcct ccattaccta gtattccacc taatttgtct
     8101 tttattttat ctaaagttcc ttctgagcct tgctgttcat ttccaccagt taagttatca
     8161 ctactggcat ccatattttc atttgtcatt gacatattat tacttgtatc tgatgtcatt
     8221 ggattagaag catcatcatt ctgagcaaat gtattaatta taggactact tgctcctata
     8281 atacttattg ttgtcgcaaa tgtgattatt aacaatattg ttgaaatcat taaagtggta
     8341 taaacaataa attatataat aatatcagat aaaattttca tataatattt aatatatttt
     8401 tttttattaa atataaatcc aatagaaata tacatataaa gaacaaagtc tagctcatta
     8461 tagatcaatg aagttcataa atagatttaa cattacctca tactatggta atttaaaaag
     8521 attaaagcac tcaatataaa aattatttat atctattatt ctgtatatta gattttaaat
     8581 atgtcgtcag gatcattcac tccctttata aaagtagaac atgtcatatc ttttagtgat
     8641 gcggtttttg cattcgcaat aactcttatg gctttatcca ttgacatacc agatttaccc
     8701 tctgacttaa cacaagctga attagtagat aaactaaatg gtctctttcc gcaatttgaa
     8761 agttatatca taagttttgc tgtaatagca atattctggg tatcgtatca tcaagtcttt
     8821 aatcatatta aaggatcgca tattatcatg gtatacctaa atttactatt tcttttatta
     8881 ataactctct tatcactttc tacttctctt gtaataaact atggaggtta tcatattcct
     8941 tatataatct actcttttat ggtaatcttg acaagctctt tgcttgccat gatatggtgg
     9001 catgctacaa aagataaact tttaatagat aaaaatttgc attcgttata tataaaaggt
     9061 gttatgataa accttatatc tattcctcta gtttttacaa tatcaataat tatttctttt
     9121 gttaatttgg atattgctga atacttttgg ttagtaattg ctccattaaa tatagccatt
     9181 aaacagaagt ataaacattc aaaataataa tagttatttt gattctagta atcaacatat
     9241 taataaaatt agtaaccctt acagtgaata cagtttttgg atttccttca attacataaa
     9301 ttttgatcct tcaatgatac atcttacaga catgacgagc agtaggtaat agttttttta
     9361 tttctcattg atctattgtt gtattgtatt tctcattatt tttaaaagtt cttagtaata
     9421 ttttcttcta taaagatata aatgctatcg ttataattat aagttggaat tccactacgt
     9481 tttgatggca taggttggag gttttaccat gtatcacttt ccggttatac ttcattaaca
     9541 tttacattcg aaaatggtaa agtaaattag gaactagaca atttttatta ttatagagaa
     9601 aagccatagc cctcgatgag ccaggtatat atagaaggct aggtatgctt aaattctttg
     9661 gaattgctgg ttaattattg aagcgagtaa ttatgtaaat tttactattt aaaaatttga
     9721 gataatttta tctttgttaa ttaattgtca taacttgaac tagtagacga ccggactgga
     9781 tgacttttgg tccagactat gactaccttt gacttatgat ttctgccaaa taggttttag
     9841 attagatttt aaagacgatt acaaagtaat ttagtgacaa ttgtatttat taatccttga
     9901 aattctcatt ctatgttttg tagttgatat ttactattaa ataataattg caaatgattc
     9961 ttcatagaaa ttatccccaa ttgttataaa ttatgataac ctacagtttt tgatgaagac
    10021 gatcttaaca actacaacct tcttaatcgc tcttctagct ataggtctta taggtatcaa
    10081 caataataat aacagtattg gtaatgtatt tgctcaagtg gatccagaag actctccaat
    10141 gatggaggat atggaaatgg atgaaggtat gatgacaatg tcaaatcaat cagaggggat
    10201 gaaaatgatg aattggactg gaacaattga tgtcaaaact actattggcg aggctttcaa
    10261 atctaaagtt actgttaaca tactagatgc aataaatgct gcacaaacaa atgttggagc
    10321 aaattctttc gtcaagaaag cagaattaac gcctgcgcat ggatatttag tatacaaaat
    10381 aatggtagta gatgaaaata tgaaaaaata taaagtcatt gtagatccag gaaatggaca
    10441 agtactgatg aagaaggaag ttacctggta tgaggatgat gaacataaaa tgaaatatgg
    10501 tgaagaaaaa ggtgacaaat acggtcacga ctataaaatg atgaaggata aagaatatta
    10561 aatctaacta tagacagcaa aataaatctt aatcacttat tatatttttt ttatttagtg
    10621 attttaaatt cctctaatac actactatcc gtagtatagt attttcctac aaaacatttc
    10681 cttggttaat aacatcgaca tttaaaaatc cataactaga tcttacataa gattcttggt
    10741 tagaaaaatg atcataatct ataccacctg taacaactat tatctgaaac attcctttgg
    10801 tggtttatag tccgttttac ctttctctat tcctaaatca tcaatggttt acacttccgg
    10861 agaaatcggt tcattgttta attgtaggtg atttaatttt ccagaaaagt aaaatgaatg
    10921 gccatttctc ttttactttt cgggacttga agtagttggt tggtaaatta ctataataaa
    10981 tccttcatta taagcagttc taatagatgt tctattgatc tataggaaat ttataatggt
    11041 tattttatgt agagaagttt attatttatt tatttatttt aattcgccat tgctgttact
    11101 actaacattt ttatcatctt cttgatcttc tttatggata aatatttttt aaatgtttag
    11161 gatcttctca tacattacaa tattctatct tttgtctgtt ttatatccaa aagccagctc
    11221 actgaaaaaa tgaaatggtc aattaataat tattaataat taaatgatta tacacattaa
    11281 ccatctgacc atactttatg tcttaaaatt ttatcttctg atttttgata ttcagacaga
    11341 tctctacagt atagaaaatt atactatatt ctttacatgt gagagtttca ctcatttcat
    11401 tagtataata taacctaaca tactatatta ataataaaca aattatacaa aaagtataag
    11461 ttttacactt tttattattt tagtattaaa aatagataaa taaattacaa tttccaactt
    11521 ttatctttta tttttaatat gaataagaat gttctatatc tattgagatt aaatattaat
    11581 aatacttata cgatagatgc tttcaacaat tataagaaat aattattatt tttaatatta
    11641 ataatgaaca aataagctaa tataataaca acttcgtctt atatagaata gggataataa
    11701 gataatgcca atgctgatgt taaaatgtaa aacttgcggt gaagttttcc caggaatcta
    11761 tattgttgaa gaaagcacta atgatgatga atttaaatca tcgattacta aaaataacat
    11821 gatacatagt tgttctagag gacaccaaaa tgaatatagt atagaagatt atatggattg
    11881 gtcgaagatt gaaatcatct aaggtaatat tagtaacata ccctgatgat tttatcataa
    11941 atgagggtaa ggagcttata ctgtcaattc cagaatatga aattgtaaaa gtttttacac
    12001 aaaaatatct taatcattca aagtatggaa tgggttcagg caaagcagaa gaaatagaaa
    12061 aatttgtaaa agaaactaat gaaatagaag aaataattgt tgatgaacat ttgagctcaa
    12121 aacagatcta taaccttgag aaattgatag gaatccctgt taaagaccgt gaaagattaa
    12181 ttttagatat cttttataca agagctacta cagttgaagc aaaattacag atccaattag
    12241 ctgaaattga atatgagatg ccgcgcgtac gtgaaaacgc aaaattgtta agtaaaagca
    12301 atgaaagagc aggaaaaggc ggtatgggag aatatatagt agatgtacag tttcgtgatc
    12361 tcaaacgaca aatgtcgttt atcaaagaaa agataaatga tgctaaaata aaacgcaata
    12421 tttatcatca ccaaagacag aaaacaggaa tacctgttgt ttctttagta ggctatacta
    12481 gttcaggaaa aacaacccta tttaatcttc ttacaaatga atataaagag acatcatcaa
    12541 atttattcac tactctttca actactacaa ggtcatttaa agttcctcat aataacaaaa
    12601 acaaggaagg agaaggagga gtattaattg tagacacagt aggttttatc agtagacttc
    12661 caacttacat gattgatgca tttaaatcga ctttagaaga atctcttggt gctgatctga
    12721 tactcctttt acttgattca tcagagaaaa tcgaagatat gagaattaaa tattcaaatt
    12781 gtctggacat tttagaagaa ttaaatgttg ataaatccaa attatttaca gtattaacta
    12841 aagtagataa aatcgatact agaatgatta atgaaattgt agagaaacta gaaataagta
    12901 gcgatgtaat agctatatca tctaatactg gatatggaat tcataagctt aaaacgttat
    12961 tgatgagaag gcaatttcta gaaaataaat ataatgagaa tacaaatgtt aagagataat
    13021 atttaaaatg tcaccataaa ttagaataat tctactgata taaaaatatc ttttatattt
    13081 ttaaaatgag aaaagtcact ctatccattt aatgtattta ttaattatta tcttaaattg
    13141 gatatattac tttcaaaatg tcttctatcc aaataaattg ctttactacg acgaatcttt
    13201 cattaatggg aatagtatta aacctattgt aatgcgatgg aaatatgtaa catcgatgct
    13261 atacaatacc tttgtaaagt agatagtaat ctagaaaaaa taatcaaaat agttggcaaa
    13321 tattctataa aaattcgaaa cgatcctttt caatctcttg tagaatcaat tatttatcaa
    13381 caactagcag gtagagctgc aaacgcaatt tataataggt ttatcaatta ctacggtaat
    13441 aaacaagtga ctcccattgg aattttaaat tcgcctaatg ataatttaaa aaaagttggt
    13501 ctatctaata gaaaaataga ttatttaaag gatctggcat cacatatcta tgatggtcga
    13561 ataaatttag aggaattgtc aaagatgaat gacgaagaaa taatcaataa attgataaat
    13621 gtaaaaggaa ttgggcgatg gacctcagaa atgtttttga tattttctct tggaagacaa
    13681 gacgtattac ctattacaga tttaggagta agaaaagcta tgcataaggt gtattcttta
    13741 tcagaacttc caaaaccaaa tacaatgatg gaaatagctg aaccatggag accttataga
    13801 agtattgcta catggtattt gtggaaatct ttatctaatt ttagtacaat tggttaatga
    13861 atgaatttag atttgtatta ataaaagcta caaatttcaa agaatttaaa acttattttg
    13921 gaaggaaaag atcgaatagt tactcctttg gatatcgtta tggaaacatt aacatagaat
    13981 ctagtcaggg tatcttttct tctactatgg aattaacata ttacatagtt aacgcttgat
    14041 taaacgagca tatacctatc tactataacc aaataagcaa gcatacaagt aaaagccata
    14101 acccactacc attgatgatg gatatatcca ataatattgg tggtgatata gatcttatca
    14161 tttgtctata agattgtata tcaattgaca ttataatatt taattattgg agaccttttt
    14221 tttctttatt attaataaat taattgccat aacccactac aaatagacag agggttggag
    14281 cgaacagaaa tggcatccta taagtagtaa ggctatggca agagaacgag tcttttgtct
    14341 atacactttt ttatagtgta atatcatata tagtttagaa tatttaaaaa tcggtaaacc
    14401 aagttattcg actaataatt atagacgaac catagcccta ctactcctac tacaaatcga
    14461 gcaaagagat acatagtata atagcaagac tatggtctgc tatggatata tatgttgaca
    14521 gattgtagta taatatatgt ccaatataat atttgtccaa tatattaata atattcttaa
    14581 ttagtacaag ttgaagtatt ttaattatat aaaaatgtcc aattatttat ttactaaaaa
    14641 ttaagtatat ttatattatt taatattttt tttgaagaaa atttctatac tatttaataa
    14701 tcataagaat aaaagtggaa tgctcatgaa tcttaatatt tttacaataa ttatttcatt
    14761 ttttgcttta ttatctgtaa gtctagtata tggagaagag aatatcaata ataataacag
    14821 ccttcaacaa aattcaaaca atacagcaac aaataataaa atactagatt acaaatctga
    14881 accatttcta ttactagatg gagaagattt tgaagatatt gctcataata atactctttc
    14941 gcttgaaaat tttgctatat atacatggat taaaactaat caaacaaatc tagtagatcc
    15001 tgctcatatt gtgaataaag gcggatttaa tactgatgag aaaggtgaaa atatgaatta
    15061 tggtatttgg ttctcaacag atggaacaat ttcgggagga tttgaaactg aatctggaga
    15121 aaattttgaa gtaaagtcta ctgcaaaata taacaatgga aaatggcatt atgttttgtt
    15181 atcttatgat ggttctctat tacgattaga tatagatgga gaaaaacaat tatcaacaac
    15241 taaaaataca aatggtgcaa tacctgatac tactggagaa caaccaataa gaattggagc
    15301 aaactcacta gatgaaagca aatttttcac tggaaacata gatgaagtta gagtatggaa
    15361 taggggatta acagacaaag aaatttcaga gatttatatg aataatgctt ttgattcaaa
    15421 cggactggta gtacatctaa attttggtgg atatagtact cctactacaa ataataattc
    15481 atcatcatta ttatcatcat ctactatcaa tcatacaaca ttaaaagaaa atctaacttc
    15541 tacaacaaca aataaagaat ctattcccga tacgattaat caaacaactt catcctcttc
    15601 tcttcctggt tctattgcca aaatcaatgc tactgattct catcgtcttc aagataacaa
    15661 aattatgaca acaacaacgt cttcttcctt caatattgca gtttcagcag attggggatg
    15721 tacagaagat acaaaaaaaa cagcagaaaa tattcaaaag aagaatcctg aaattgttat
    15781 tgcagcagga gacctaagtt atgaggaatc tgcaaattgt tggtttgaaa taatacagcc
    15841 atttaaatca aagatgaaga tagccatggg agatcatgaa tacagtgata ctagtggtgg
    15901 gacaataggt gtaatatatc aatatctgaa acctttgaat ttaacaaaaa catattattc
    15961 ttttgatatg aataaggttc attttgttat tatagatcct tatattgatt ataatgctgg
    16021 ttcatctcaa tatcacttta ttgaacaaga tttaaaaatt gcttctacta atccaaaaat
    16081 agactggaca tttgttgtag aatctatacc catttatact tcacctgcac aacatcctgc
    16141 taattctact attagagata tttatcatcc tctttttgat aaatatggtg tagatttagt
    16201 atttactagt gacaatcaca attatcaaag gacttttcca ttaaaatata atagcaaagg
    16261 tggagatagt tctaataatc ccattatagt agatagcaaa caaaataatt attataataa
    16321 tgacaaggga ggagtcattt acttgattac tggtactgca ggaagatctc tttatgaaat
    16381 taaacaacaa gcgccttttg ttgcaaagca gagtgatgaa cattttggat ttctaaatat
    16441 cgatatttat gataaaatgc tgaagggaac tttctatgca aacgaaaggg aattacctca
    16501 ccattattat gtaaattttc aaaataacat aataatagat cattttacaa tttcaaagac
    16561 gaattcacct aataataatc tttgataaac ttagacttga ctcaatgtga ctattagtat
    16621 tcgcacctgt attagttagt atgctatata taaaagggga tgatgatgct tttgtgtctg
    16681 catctatatt tagtaatgat tgttgttttt gttcttgtct aatactgtta agcaacaact
    16741 agataaatct gatagttgta tattgtatat aatgttagta tatatcgtag caatattatt
    16801 gggactgtgt taaaattcat aaaatataat atttaatagt tagagataat ttaattgtca
    16861 tagcctgaac aagcagatga accatatttg gatgatatgt ggtccagact acaacaacaa
    16921 gacttgtaat agttttgcct atggttatga aaaaatgata gaaaggatat ttataatttt
    16981 ctcaaaacct cattatatat tttgaaatca gttaaaaata atttatgtta ccaggataaa
    17041 aaataaaaaa agtagggatt gttgaagcat tgtgctactg tcacaataat aacactatca
    17101 gcaggatccg atggtccgaa tactgttgtt ccccgaccgt cttgcgtagt tagagcaatg
    17161 ttttgaatgg aaccgagatt ttcaggaact ttaattattc acttccaatt catttgttaa
    17221 gttttggaat tccttccttc tatcactgaa gaattgaaat actaaggctg ctgctgttaa
    17281 agttaaacct gctacaattc ctatagtaaa taattcacaa cccacgttct atcaaatata
    17341 tttacaacat tctattattt taatttattt ggaaaacgat ttaactgatt ataatataat
    17401 gtatgattaa tatcaaagcg attgataata actggtttcg taataaaata aaaaaagtat
    17461 tatcaatttt tagattattg gttaagaatg tagtagaaag tagaattact attattatta
    17521 aaattatttc ataaatttaa taatgctttg ttttagagga tatactcttt agttgacttc
    17581 tgtttaaatt tcataacttt ccttaataga aagtattgaa ataatatata gcaagtacaa
    17641 ttaaatatat ttattttctt ttagtttaaa aagttactta tatcttttat catttactgg
    17701 ataatatgtt atttatatta gtataatatt taaaaaaaat tttgattcat ttaacaattc
    17761 ataaacgaat aataatttta attttaatac aatcattacg aaaattacta tacagttttt
    17821 gtctaataat aggttattac ttgcgagaca ataaaaaaat aacaaataag aacagaataa
    17881 tcgatgatca tagcaactgg cgaacatctt cgcgagtagc agattattta aatcgtgtag
    17941 ataaaatccc tcatagagca gaaggagaac ttttattatg tgatttcata ccaaaaaaga
    18001 acaagaagaa ttcttgattt aggaacagga aatggaagat taataaaatt gttaaaagaa
    18061 aaaataccaa ctgtaaaatc tgtaacaata gatttttctc ctcctatgct taatgaattc
    18121 aaaagagaat ttctgaatga tgacacaatt aagattatag aacatgactt aacgtatcct
    18181 cttcctacga atttaggaaa atttgatgct attgtttcta attttgcaat acatcatctg
    18241 atgcataatc gtaaaaaaga cctatactct gaaatatttg ttattcttaa accaggaggc
    18301 attttttgca atcttgatca tatttcctca gagtctaaaa aaataaatca atatttcaga
    18361 aaagtaatgg gacgaagacc tataaacaaa gaacatggta aaagactaac taaaattcat
    18421 attcaattgg aatggttatt ggagatagga tttgtagatg tagattgcta ttggagatgg
    18481 ttagaatttg cgttgttgat aggattcaaa ccctagactt gatttagtaa taa
//
LOCUS       DASVEV010000304        14060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_168874, whole genome shotgun sequence.
ACCESSION   DASVEV010000304 DASVEV010000000
VERSION     DASVEV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168874"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF222_13195"
     CDS             complement(<1..245)
                     /locus_tag="VF222_13195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX7143425.1"
                     /translation="MSSFILFTLLFSYTNNTGYALGQISHNLLEQNNSSELKIQDLFS
                     QTSKSVVQITVIDPLSNAQQGLGSGFLYDKDGHIITN"
     gene            1034..1255
                     /locus_tag="VF222_13200"
     CDS             1034..1255
                     /locus_tag="VF222_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143426.1"
                     /translation="MGITAEVSNNHTSLNETDIKMINKNNHTSLNETDIKMINKTESK
                     NSSSETCVMPKCPSEKVCIQVCPESLPPV"
     gene            complement(1275..2585)
                     /locus_tag="VF222_13205"
     CDS             complement(1275..2585)
                     /locus_tag="VF222_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7143427.1"
                     /translation="MITVCGIFLVIPMFENTYAEDNFEKQFLFNYFYHNQNIDNRYLE
                     SNNVIPNQFIIYLRDNQENNLIDPIEFYNSELKDTGTELLYVYYHVVKGLAIKIPNEK
                     VLEQLKNNPLVEYLGNDRKISAFIDTPTGNQIIPDSVNRVDGESLNNININSNFVDAD
                     IAILDTGIDLDHADLNIFHEKSFIPGTINADDDHGHGTHLAGVAAAKDNSFGSVGIAP
                     GARLWAIKVLEGTGTGEISTLIKGLDYINQNRNQVDVAVLSLGCECKSGALDIAINNS
                     IKAGTTIVVAAGNVGKDAITFTPANNPEVITVSAIVDTDGKCGSKGPSTPYGADDTLA
                     SFSNYGDVVDISAPGVEIYSTFKSNSYTKLTGTSMAAPHVAGAAALYISSHPGASPND
                     VKSYLVTSGTNLSDLCDGNGHGYFAGDKDSFHEPLLYIGANKVT"
     gene            3382..3666
                     /locus_tag="VF222_13210"
     CDS             3382..3666
                     /locus_tag="VF222_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143428.1"
                     /translation="MVNRVLNVQDFFFFPGKTARYEGHFLTSSFDPILIEITKKINES
                     ISEIPKYQLLLNVQNSKSYEISDCRIYRFENRNYEHTMCYFLSNDIEEIN"
     gene            3884..4543
                     /locus_tag="VF222_13215"
     CDS             3884..4543
                     /locus_tag="VF222_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143429.1"
                     /translation="MINNGKLYFPINVVAISDILTLEKDLGNQIPNKSWMLYLLELFN
                     VQNFPVELNLICQFDNQNYFEAIFLGINRYRFKNVLPVIYHSYQRLIHLDRKNDQINF
                     ILTDQTINKSEIFNLDIKDTNFDYKTLNQFTGIEWWNKTGPFIYPIRYQIEISQLMFG
                     LVDKNDSESIIFFPHNVLIPNKDGYKNEYPITFRNVRLNDNCICYNISYGLCDNGLSY
                     S"
     gene            complement(4916..5458)
                     /locus_tag="VF222_13220"
     CDS             complement(4916..5458)
                     /locus_tag="VF222_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143430.1"
                     /translation="MDENNARDKRKTSTFTLRIDEDITKLLQNESQLHDISLNTLINK
                     ILKRYVEWDSYEPKVGMIPMAKPVITTLFNMMSEEEILDLVSNFGKNVVQDIAYFMQM
                     KSDPDSFLTWFEIRMRRSFVEFNHLQENDRHTYILKHDLGYNWSLYHKKILERIFNEI
                     FNKPVKIAISDFMLTIQFER"
     gene            complement(5537..6067)
                     /locus_tag="VF222_13225"
     CDS             complement(5537..6067)
                     /locus_tag="VF222_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143431.1"
                     /translation="MNVDSKRTKPLTFRIETKILEKIREEGGNNQISVSNFINKILKR
                     YVEWDSYEPKVGMIPIPKLLVEKLFEKRSTQEIIDLATNVGKNELEDILLFMNKKINW
                     ILFLNWFEMRLQNSSIEIVHTVEENKHTFIMKHGMGENWSLYHKTIFELISNENFKNP
                     VQIKYNSRIISIEFYE"
     gene            6774..7355
                     /locus_tag="VF222_13230"
     CDS             6774..7355
                     /locus_tag="VF222_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143432.1"
                     /translation="MSSFGVDTLGGLTEKLKQLITDTQNAYETLIDSTQRYKQGKLND
                     KDYFSSIGKYLIASSAMNFLAIKVILEIKSAMDKTSSIKNPTGGIADPNSNASSSVGF
                     GVGGFVGGGGSVGGIEKSRIQGSGSSQQQYSSSSIEPTLKPVDIEIEKPSQDITTSDV
                     DSLKDNKTFKNCIVCGVLIPTKAKFCSKCGNAQ"
     gene            7383..7808
                     /locus_tag="VF222_13235"
     CDS             7383..7808
                     /locus_tag="VF222_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7143433.1"
                     /translation="MMKVFDGRSAAEEYMSTHTLTFSTPEMTLKKFVVWLSEIVENNG
                     EEVSRIILYLQTRDKENEESQSDEISINNPINFSDLLPDIDEAFKPTGAITDMFNKTE
                     SSLSKNIEYSTNPESKFCIECGNKLRADSKFCTKCGARF"
     gene            complement(8033..8302)
                     /locus_tag="VF222_13240"
     CDS             complement(8033..8302)
                     /locus_tag="VF222_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143434.1"
                     /translation="MADANFRVQSHKFTQDNIQATLVCSKCDEIFSKEFLLDLEGQTI
                     EFRCPVCGVSGEADLPYKTAEEREEFDDDLDEVEEDAFDEFEEDY"
     gene            complement(8426..9022)
                     /gene="folE"
                     /locus_tag="VF222_13245"
     CDS             complement(8426..9022)
                     /gene="folE"
                     /locus_tag="VF222_13245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX7143435.1"
                     /translation="MTTNSRVQKTGTINKNKIEKLIRQLLVELGENPNREGLIGTPKR
                     IAEMYEEIFSGYKMNSELDVSFSEETNAIIAKDIQFYSMCEHHMLPFYGKIHIAYVPN
                     GKVFGLSKLVRLAEKYSRRMQIQERLTTEIADEISKRGVSGVIVIAEGEHLCMKMRGV
                     RNNNSIITIAYRGIFEEKGIRENILNLIYTRQNNIINL"
     gene            complement(9030..9776)
                     /locus_tag="VF222_13250"
     CDS             complement(9030..9776)
                     /locus_tag="VF222_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX7143436.1"
                     /translation="MTKQNMPINEKKIQLSEIFTSIEGEGILCGTKTMFVRMAGCHLK
                     CYWCDTSYALAMNSGNTYPINEVKKMIKDHLQNNTYKINFTGGEPLIQYEAVKDLAKF
                     IKEELGLRTYIESSCYDIDRFNKIIPYIDICKIEFKTKDSQAVDIKHYRYLLNNEIEC
                     LKTAIDNKKITFIKIVVSNSTSIKEFRELIEVIFNTIKCDDLAGFIIQPTSTINEPTV
                     SRLLQFYDLIYPIYNEVRVIPQLHKIIGAR"
     gene            complement(9748..10485)
                     /locus_tag="VF222_13255"
     CDS             complement(9748..10485)
                     /locus_tag="VF222_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX7143437.1"
                     /translation="MKFKTKKNKISSFSTAVCIVSGGIDSICTAAYLKKQNFDLYIIT
                     FSYGQRANLEIEYAKKFAKYLDSKQHKIVDISFMKDIYGETNILTDDKQKIPKNFEYS
                     IIAPIRNAIFITIATAWAMNIGAKIIAYGAHTGDNNYPDCRPEFIKSISKTLNLGDID
                     VIRSGLRKKISIWSPAIDGLDKSKIIRIGYKILGNKIFETWSCYSNGIDTKDGKKLHC
                     GICESCINRRRAFRKVSIDDKTEYANQ"
     gene            complement(10540..11571)
                     /locus_tag="VF222_13260"
     CDS             complement(10540..11571)
                     /locus_tag="VF222_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HEX7143438.1"
                     /translation="MSSNTIDTKLSSLMWVEKYRPKRIEQIVNQKDILTSLENLIKKP
                     NEIPHLLFAGPAGVGKTTTALCLSMELLGEEWKRDTLELNASDERGIKMVRERVKEFA
                     AVIKLPSGTNEKGEYFNKDEKPYRIIILDEADEMTSEAQTALRRIIEDSSKTTRFIII
                     CNYLSQIIEPIQSRCAIFHFTRLEEVDVLEYLRMICNQEKVDFQQEALSQIYRYTEGD
                     LRHSINILQTASGYGQVSIKNVTSTIGLSGKAKVGEIIRLALLGKFNDARIRLLELLY
                     AYGLSDTDFFKYAYQEIKDLDIKNIDEVMSIMAEYDYRLSVGAHSEIQLTAFLAQLCK
                     IGSNIKLKK"
     gene            11831..12298
                     /locus_tag="VF222_13265"
     CDS             11831..12298
                     /locus_tag="VF222_13265"
                     /inference="COORDINATES: protein motif:HMM:NF017704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143439.1"
                     /translation="MEETRITFKKNFSINIDGLNLEAKEGEIGSIPRWLAQILEENNS
                     LEIQDTDVLIYISRSLNRERISKPHDLSGIDIDFYIRANDFIKRLKDKEKESIVVSLN
                     SFVTSRIEKIVKLAAASALSVDLEKKLSAEEKELYNFIHKFSSEFKQRAVNRY"
     gene            12295..>14060
                     /locus_tag="VF222_13270"
     CDS             12295..>14060
                     /locus_tag="VF222_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HEX7143440.1"
                     /translation="MSELQKTESALVNDLEEFLRAFKDRIGNYKYFDRINNLMAANST
                     SLVVDYIDFDTFKPDLAKLITNKPDEMFDAFNRAIFNVLYEIHPDYADEIQNNIKVRI
                     GNYTVQKSLREINAEVINKLVGVSGMVVRSSEVKPLAKKIGYRCLSCGEITEAQLRGL
                     VLKKPLRCGKCSEKELEMDPDTSIFIDFQMVRLQELPEDLPAGHLPHYLEVTIIGDLV
                     DQCRPGDRILLTGIVRIEQEQLSQLIKTSLFRLRMEGNNIEYLGGRTADKDSRTIERL
                     EISNEDEKHIFALARKNDAYDILISSYAPHVYGHEVIKEVILLLIVGSVTKRLEDGSA
                     RRGDINVLLVGDPGTAKSEMLKFAAKIAPRGLYTSGRGSTAAGLTAAVIRDKSGIMML
                     EAGAVVLGDQGLVCIDEFDKIKPEDRSALHEVMEQQTCSVAKGGIVATLNARTSILAA
                     ANPLYGKYDPFKNITENVNLPIPLLTRFDIIYVIRDIPEKEKDHRIASHILELHRESE
                     STGQYLIDIDLFSKYLAYSKTMEPKLTVEAIDKIRDYYMKMRNVESEGMITVTPRQLE
                     GLVRLATARSKLLLKDYVDSEDA"
BASE COUNT         4951 a         2049 c         1863 g         5197 t
ORIGIN      
        1 ttatttgtta ttatatgacc atctttatca taaagaaaac ccgaacctaa accttgctgt
       61 gcgttagaaa gaggatcaat aacagttatt tgaactacag atttagacgt ttgtgaaaac
      121 aaatcttgaa tttttagttc actagaatta ttttgttcta gtaaattatg tgatatttga
      181 cctaatgcat aacctgtatt gttggtataa gaaaacagta atgtaaataa tataaaagat
      241 gacaaatata aaatcacaga aattttaatt aacatttact atttaattaa taaagtgtaa
      301 tattaattta atcaatactc acaatattct attagaaaat ctatgttatc ataattctac
      361 ttgatgtttt actgctcttg ataacaaagg ttaaggttat ttgttttaga aagtccatac
      421 caattgataa ttagtgtatt tcttcgtagt tttgcttatt gatatggtat tttaatacat
      481 atgttatctt gaattctgtt gaatctttca gtgtatatat gtgtcttgag tatatagaaa
      541 gtataggata gaatttattt tcttccaatg taatagtatt taacctgtct gtaaacattc
      601 tgtaactctc ctcctgttgc caattgtaat aaatagactg tgttgcaatc tgtaagtatt
      661 tgattgactt ttctatgatg ttataatgaa atctaacaac attaaggatg cgatacaatg
      721 actgtgctaa ttttacatat tttgattgtt gtttgttcaa cattgaaagt gaaagtgggt
      781 ccgatatgat gtttctatat aaccgagata atatatgtaa tgatttatat aaataacaat
      841 tttgaattcc tatcttgatt taacttttaa tatgtactta aaaattgaac aaattttaaa
      901 tgaaacgaaa atttttaata ttaacaactt aaataaattt ttaaatataa catgtttact
      961 ctattgtatg catgtgcatt tgggttatat tattataatt ctatttatta ttagtataac
     1021 ggtaatcact tccttgggta ttacagctga agtatctaat aatcatacat ctttgaatga
     1081 aactgatata aaaatgatca ataaaaataa tcatacatct ttgaatgaaa ctgatataaa
     1141 aatgatcaat aaaacagaat ccaaaaactc ttcttcagaa acatgtgtaa tgcctaagtg
     1201 tccaagtgaa aaggtatgta tacaggtatg cccggaatct cttcctccag tttaatattg
     1261 ctctacagat taaatcaggt cactttattc gcgcctatat ataatagagg ttcatgaaaa
     1321 ctatctttat ctccagcaaa ataaccgtga ccattaccat cacacaaatc cgaaagattt
     1381 gtgcctgaag ttactagata acttttgaca tcatttggag atgctcctgg gtgtgaactt
     1441 atgtacaatg ctgctgctcc ggctacatga ggagcggcca tacttgtacc agttaatttt
     1501 gtatatgaat ttgatttaaa agtagaatat atttctacac caggtgctga tatatctact
     1561 acatcaccat aattactaaa acttgccagc gtgtcatcag caccataagg tgttgatggt
     1621 cccttacttc cacattttcc atcggtatcg actattgcag atactgttat tacctccgga
     1681 ttatttgctg gtgtaaaagt tatagcatct tttcctacgt tacctgcagc aactacaata
     1741 gtagtacccg cttttattga attatttatt gcaatatcta atgcgcctga tttacactca
     1801 caacctaaac ttaaaactgc aacgtcaact tggtttcgat tttgattaat gtaatccaat
     1861 ccttttatta gagtagaaat ttctccagtt cctgtgccct caagtacttt aatagcccat
     1921 aatctagctc caggggcaat acctacactg ccaaaagaat tatctttagc agctgcaact
     1981 cctgcaagat gcgtaccatg accatggtca tcatcagcat taattgttcc tggaataaat
     2041 gatttttcat gaaatatatt taaatcagca tgatctaaat ctattcccgt gtctaaaatc
     2101 gctatatctg catcaacaaa atttgagtta atattaatat tgtttaaact ttcaccatct
     2161 actctattta cactatcggg tataatctga tttcctgtag gtgtatctat aaaagctgat
     2221 atttttctat catttcccag atattctacc aatggattat ttttcaattg ctctaaaact
     2281 ttttcgttag gaatctttat tgctaatcct tttactacat gatagtagac atataataat
     2341 tcagttccag tatctttcaa ttcagaatta taaaattcaa ttggatctat taaattattt
     2401 tcttgattat ctcgaagata tataatgaat tggttaggaa ttacattatt actttctaaa
     2461 tagcgattat caatattttg attatgataa aaataattaa aaagaaattg cttctcaaaa
     2521 ttatcttctg catatgtatt ttcgaacata ggaataacta aaaagattcc acatacagta
     2581 atcactacta aaaagttcct tctaaaatta tactgaagat tcattttgca attaatagac
     2641 aaatttaagt ttaatttatt aatactttta aaaatattat tatatataaa ttgaatagta
     2701 aaataaatat ctgattcttc cctactttct ctctacttta aaacaataat aaagttataa
     2761 aagaaaataa tggaaaaaaa tatataaaat aaaattataa tggtttatgt aattagtatt
     2821 ataataacaa tgaaatatca gtaaaaattt ggatgctaaa gttattaaaa tggtaaaatt
     2881 ttgtttcatt aatcattttg atggatgaga attaaccata gccgtcaata gttcgaatag
     2941 atataaaaga taataatcta attgagtaaa taaagtgaac gggtagaatt aaagaaaaat
     3001 tacagacaat gaccatcgta ttgcattatt tatttgatag aatcgtttag tggaaaaagt
     3061 atagagaaga ctactgaaac cgcagaaatc atatagatgg ttatccaagc gatatttagg
     3121 aagagagaag tatctatata gaattccaaa tgagattaga ataataatta aagtaaaatc
     3181 aaaaagaatt tatagtttaa caagttaatt atttactatc gttaaagagt atatttccat
     3241 ttttcatggt ttaagataat ttgttatata aagataataa ttatgtaata ttttgatatc
     3301 attattgaaa ttatacttgc aaagtaaatg actagatatt tcttgttatg atttaaataa
     3361 gtaagattac ttaatttttt tatggtaaat agggttttga atgttcaaga ttttttcttt
     3421 tttcctggta aaactgcccg atacgaagga cactttctta cctcatcatt tgatccgatt
     3481 cttatagaaa taacaaaaaa aataaatgaa tctatctctg aaattcctaa atatcaatta
     3541 ttgttgaatg ttcaaaactc aaaatcttat gaaatttcag attgccggat atatagattt
     3601 gaaaatcgaa attatgaaca tacaatgtgt tattttttaa gtaatgatat tgaagagatt
     3661 aattaagtat tattattatt attattgatt tcacatatta atattttcat catttttctc
     3721 tatcatgtta ataatcacct atgtttagat tgattatgaa tgaatttatt attgtatttc
     3781 aaatattaat tatttacata attttactag tctgtaaaga tatattaatt aattatagtt
     3841 taagtataat ttagtgcatt attggttagg atcattttgt agagtgatta ataatggtaa
     3901 attatatttt ccaataaatg ttgtagctat atctgatata ttaacactag aaaaagatct
     3961 aggaaatcaa atacctaata aatcttggat gctatattta ctcgagctat ttaatgttca
     4021 aaattttcca gttgaattaa atctcatatg tcagtttgat aatcaaaact actttgaggc
     4081 aatttttttg ggtataaata gatacagatt taagaatgtc ttacctgtaa tttatcattc
     4141 ttatcaacgt ctaattcatt tagatcgaaa aaatgatcaa ataaatttta tattaacaga
     4201 tcaaacgata aataaatcag aaatatttaa tctcgatatt aaagatacaa attttgatta
     4261 taaaacatta aatcaattta caggaattga atggtggaat aaaacaggcc catttattta
     4321 tcctatacga tatcaaatag agatttctca acttatgttt ggtttagtag ataaaaacga
     4381 ttcagagtca attatatttt tccctcataa tgtactaatt cccaataaag atggttataa
     4441 gaatgaatat cctattacat ttcgaaatgt tagattaaat gataattgta tatgttataa
     4501 tattagttat ggcttatgtg ataatggatt aagttattct taaatcttca ttatttatat
     4561 aacttaatat tataaaattc aagaaaataa tattatagat tcatcatttt gttatgcaca
     4621 tacttttaac cattctattt atcagtcaaa gttggagatc tatattagag aattaatcaa
     4681 gacaatgtca ttatactatt aaaaacttac atgtagacct tttcgtatct tttaaaatcc
     4741 tactagaatc atgattttat tttatactct aataatagag acttatttgt attgtacctc
     4801 aatggattaa aggaaataaa tatactttaa aagcaacggt tattaaattt tagaaaactt
     4861 gaggaagaat aacttttcaa gtaaataatc ttagaataca tttattaaaa tttatctatc
     4921 tttcaaattg aattgtaagc ataaaatcag atattgctat cttaacaggt ttattaaaaa
     4981 tttcattaaa gatcctttct aatatttttt tgtgatataa agaccaatta tatcccaaat
     5041 catgttttaa gatatatgta tgacgatcat tttcttgaag gtgattgaat tctacaaaag
     5101 accttctcat cctaatctca aaccatgtta aaaatgaatc tggatctgac ttcatttgca
     5161 taaaataagc tatatcctga actacatttt tgccaaaatt tgaaactaaa tctaatattt
     5221 cttcttcgct catcatatta aacaatgttg ttataactgg tttagccatg ggtatcatgc
     5281 ctactttggg ctcatatgaa tcccattcta catatctttt taaaatttta tttatcaaag
     5341 tatttagact tatatcgtga agttgtgatt cattttgtaa taattttgta atatcttcat
     5401 ctattcttaa tgtaaaagta cttgtctttc ttttgtctcg agcattattt tcatccatgt
     5461 ttgtaatatt acgattttat taaagataaa tttttgtctt tattgatgtg ataaacaatt
     5521 tctataaaaa ttctaattat tcataaaatt ctattgagat gattctacta ttatatttta
     5581 tttgtacagg atttttaaaa ttctcatttg atattaattc aaaaatagtt ttgtgatata
     5641 aagaccaatt ttctcccatt ccgtgtttca ttataaatgt atgtttattt tcttctactg
     5701 tgtgcacaat ttcaatagat gaattctgta gtctcatctc gaaccaattc aaaaacaaaa
     5761 tccaatttat tttcttgttc ataaacaata atatatcttc caattcattt tttcctacat
     5821 tagtagctaa atcaataatt tcttgtgtgc tccttttttc aaaaagcttc tccactaata
     5881 actttggaat gggtatcatg cctactttgg gctcatatga atcccattct acatatcttt
     5941 ttaaaatttt attaataaaa ttactgacac ttatttgatt atttcctcct tcttccctaa
     6001 ttttctctaa tatcttagtc tctattcgga aagtaagggg ttttgttctc ttagagtcaa
     6061 cattcataat ataactcgaa tatactatac cttacttcca tttaaaaatt agttctctct
     6121 ctttcattaa tcccatttac tcaacaattt cataacttgt atattttccg gtttttatca
     6181 tcttatatgg tttaataaaa atttaataaa tttcagagaa attagaaaag aaattaatgg
     6241 aatgaataaa gtcataataa tatctataca atttattctt tcaaagtcat ttctttttgt
     6301 ataattgctt aaatattatt catgacataa gaacaggaat acatgggtgg actaagaaaa
     6361 tctacaacta taatgccata gtaaatcaaa tagtcctgct cataattgta tacctgatta
     6421 tataattacc catttgtaac catataaaaa tgcaaaatca tcttgataat aaaaacaaca
     6481 aatagattga tgtatataat caaatgtgct ggctgaaaaa gctatccacg tgatgtagag
     6541 aatgatatat aatatcccta aatgtcccag ttattccaga aaaaagtggt atcgtcaaat
     6601 taaaaaaaga aaatcctcta ctactctata tttatctttt aacattaacg aatctttaaa
     6661 tattgacaat actgaaattt attttgaatc aatcttgctt aaaaatatat aatattgttt
     6721 tttatatatc tttttcaaac tttattaaac ccaaactaac agtatttttt ataatgtcct
     6781 cttttggggt tgatacgctc ggtggattga ctgaaaaatt aaaacaatta attactgata
     6841 ctcaaaatgc gtatgaaacg cttattgatt ctacacaacg ttataaacaa ggtaaactga
     6901 atgataaaga ctatttctca agcattggaa aatatctaat tgcaagttct gcaatgaatt
     6961 ttctcgctat caaagtcata ttagaaataa aatcagcaat ggataaaaca agttcaatta
     7021 agaatcctac tggtggtatt gcagacccta actcgaatgc gagctcttct gtaggttttg
     7081 gggttggtgg attcgttggt ggaggaggtt cagttggcgg tatagaaaaa agtcgtattc
     7141 aaggtagtgg tagtagtcaa caacaatatt cttcatcatc aatagaacca actcttaaac
     7201 ctgttgatat agaaatagaa aaaccatctc aagatataac tacatcagac gtagattcct
     7261 taaaagataa taaaacattt aaaaattgta tagtttgtgg agtcttaatt ccaacaaaag
     7321 caaaattttg tagtaaatgt ggtaatgcgc aataattaaa aacaagttat aaccttttat
     7381 tcatgatgaa agtatttgat ggtaggtcag cagcagaaga atacatgtct actcatactt
     7441 tgactttttc tacacctgaa atgacactta aaaaatttgt agtatggtta tcggaaattg
     7501 ttgaaaataa tggcgaggaa gtatctcgaa ttatacttta tctacaaaca agagataaag
     7561 aaaatgaaga aagccaatca gatgagatat cgattaataa ccctatcaat ttttcagatc
     7621 ttttacctga tattgatgaa gctttcaaac ccacaggagc aataactgat atgtttaaca
     7681 aaacagaatc atcattatct aaaaatattg aatattctac aaatccagaa tcaaaatttt
     7741 gtattgaatg tggtaataaa cttagagctg attctaaatt ctgcacaaaa tgtggtgcga
     7801 gattctaaaa tttaaataca taataacagt aaaattaagc ctagttttcc tattcgctat
     7861 tcctattttc cataaattct ttttctttta ttgataaata cactcattac aaattgattg
     7921 agagaaaaaa ttaacaaata gaatctgacg ttttacaatt tgacagattt gcacatctta
     7981 aatttaaatc gaaaaataac atatgtaata aatatttcaa ttattagatc ctttaataat
     8041 cttcctcaaa ttcatcaaat gcatcttctt ctacttcatc taaatcatca tcaaattctt
     8101 ctctttcttc tgctgtttta taaggtaaat cagcttcacc tgaaactcca caaacagggc
     8161 atctaaattc tatagtttgt ccttccaggt ccaataaaaa ttccttggaa aatatctcat
     8221 cacattttga acaaactaat gtagcttgaa tattatcttg agtaaattta tgagactgga
     8281 ccctaaagtt cgcgtcagcc attcttctta cctatagaaa atttacttca ttataagcct
     8341 ttcattacaa aaataatatg taaatataaa taaaaaacaa actttatatt ttaatgtgat
     8401 taataattta agttatttat tatttttata aattaattat attattttgc ctggtataaa
     8461 ttaaattaag aatattttct cttatacctt tctcttcaaa tattcctcta tatgctatag
     8521 taattattga attgttattt ctaactcctc tcatcttcat acaaagatgt tccccttcag
     8581 ctataacaat cactccacta actcctcttt tagatatttc atctgctatt tcagttgtta
     8641 atcgttcctg aatttgcatt cttcttgagt atttctcagc taatctaact aattttgaca
     8701 ggccaaatac tttgccattt ggaacgtaag caatatgaat ttttccatag aaaggaagca
     8761 tatggtgttc acacatgcta taaaattgta tatctttagc tattatcgca ttagtttctt
     8821 cactaaaact aacatctaat tctgaattca ttttatatcc agaaaaaatt tcttcgtaca
     8881 tttcagcaat acgttttggc gttcctatta atccttctct attaggattc tctcctaatt
     8941 caaccaaaag ctgtctaatt aatttctcaa ttttattttt atttattgtt ccagtttttt
     9001 gtactctact atttgttgtc atcttttatt tatcttgctc ctattatctt gtgcaactga
     9061 ggaattacac gaacttcgtt atatattgga taaataaggt cataaaactg caataatcta
     9121 ctcacagttg gttcatttat cgtagaagtt ggctgtatta taaatcctgc taagtcatca
     9181 cattttatag tattaaaaat aacttctatc aattctctaa attctttaat agatgtggaa
     9241 ttagatacga caatttttat aaaagtgatt tttttattgt ctattgcagt ttttaaacat
     9301 tctatttcat tatttaatag atatctataa tgtttaatat caacagcctg tgaatccttt
     9361 gtcttaaatt ctatcttgca aatatcaata taaggaataa ttttattgaa tctatcaata
     9421 tcataacaag aagattcaat atatgtcctt aaacctaatt cttccttaat gaattttgcc
     9481 aaatccttta ctgcctcata ttgaattaac ggctctccac ccgtaaaatt aattttatat
     9541 gtattatttt gcaaatgatc ttttatcatt ttttttactt cattgattgg ataggtattt
     9601 ccactattca ttgccaaagc ataactagta tcacaccaat aacactttaa atgacatcct
     9661 gccattctaa caaacattgt cttagttcca catagtattc cttctccttc tatacttgta
     9721 aatatttcac ttaattgtat tttcttttca ttgattggca tattctgttt tgtcatctat
     9781 gctaaccttt ctaaaagctc ttcttctatt tatacacgat tcacaaattc cacaatgtaa
     9841 ttttttacca tcttttgtat ctataccgtt tgaatagcag ctccatgttt caaaaatttt
     9901 atttcccaag attttataac ctattcttat tatttttgat ttatctaacc catctatagc
     9961 cggtgaccat atactaatct tttttctcaa acctgatctt attacatcaa tatctccaag
    10021 attcaaagtt ttacttattg attttataaa ttctggtcta caatccggat aattattatc
    10081 tcctgtgtgt gctccgtagg caattatttt tgctcctata ttcattgccc aagctgtagc
    10141 tatagtaatg aaaattgcat ttcttatagg agctattata ctgtactcaa aatttttagg
    10201 aattttttgt ttgtcatcgg taagaatatt ggtttctcca taaatgtctt tcataaatga
    10261 tatatctact attttgtgtt gttttgaatc taaatattta gcaaatttct ttgcatattc
    10321 aatctctaaa tttgctcttt gaccataact aaaagtaatg atgtatagat caaaattttg
    10381 ctttttcaaa taagctgcag tacagataga atcaattccc ccactaacaa tgcatactgc
    10441 agtagaaaat gaacttattt tgtttttttt cgttttaaat ttcattatta ataacaatcc
    10501 tttttttaca gaaaaagata gaatttcttt ctagttgggt tattttttta gtttaatatt
    10561 agatccaatt ttacataatt gtgcaagaaa tgcagttaat tgaatctcgg aatgcgcccc
    10621 aacagaaagc ctataatcat attcagccat gattgacata acttcatcaa tattctttat
    10681 atccaaatct ttaatttctt gataagcata cttgaaaaag tcagtatcag ataatccata
    10741 agcatataat aattctaaaa gtcttatacg cgcatcatta aatttaccta atagtgctaa
    10801 tcgtattatt tctcctactt ttgctttacc agaaagtcca attgtagatg ttacattttt
    10861 aattgaaact tgtccatatc ctgatgctgt ttgaagaata tttattgaat gtctcaaatc
    10921 tccttcggta tatctgtaaa tctgtgataa tgcttcttgc tgaaaatcta ccttttcctg
    10981 attacaaatc attcttaaat attctaatac atccacctct tcaagtcttg tgaagtgaaa
    11041 aattgcacat ctgctttgga tcggctcaat aatctgagaa agataattac atataatgat
    11101 aaagcgagta gtcttagaac tatcttcaat tattcttctt aaagcagttt gtgcctctga
    11161 tgtcatttca tctgcttcat caagtattat tattctataa ggtttctcat ctttattgaa
    11221 atattctcct ttttcattgg ttccacttgg aagtttaatt acagctgcaa attcttttac
    11281 tctttcgcgt accatcttta ttcctctttc atcagaagca ttgagttcaa gagtatctct
    11341 tttccattct tctcctaata attccatgga taaacataat gctgtagtag tcttacccac
    11401 accggcaggc ccagcaaata atagatgtgg tatttcatta ggttttttta ttaaattttc
    11461 aagtgaagtt aaaatatcct tctggttaac tatttgttca attcttttag gtcgatattt
    11521 ctctacccac attaagcttg aaagttttgt atctatagtg tttgaggaca tttgatttga
    11581 gtacctaatt ctatagttaa tatcttcttt tcccttaatc tatactataa taatatgata
    11641 ataagataat ttattatact aattactcaa gattattttg ttaataagaa ttattagtct
    11701 tgaatgaaat aatatcataa agagattgga ccaagttata atagctaata ataatgataa
    11761 tgttgaccgt tacgatcatt ttaaaaaaca aatggatttt attcaaaaat tttattatat
    11821 taaatattta atggaagaaa cacgaatcac cttcaaaaaa aatttctcta tcaatattga
    11881 tggacttaac cttgaagcaa aagaaggaga aataggttca attcctagat ggttagcaca
    11941 aattttggag gaaaataatt ccttagaaat tcaagacacg gatgttctaa tttatatttc
    12001 aagatcttta aatcgagaaa gaatatcaaa acctcatgat ctttcaggga ttgatataga
    12061 tttttacatt cgtgctaatg attttatcaa aagattaaaa gataaagaaa aagaatctat
    12121 tgtggtttct ctaaattcat ttgtaacttc ccgaatagaa aaaattgtta aattagcagc
    12181 tgcgtctgct ttatctgttg acttggaaaa gaaactttca gcggaagaga aagaacttta
    12241 caattttatc cataagtttt cttcagaatt taaacaaagg gctgtaaata ggtattgagt
    12301 gaacttcaaa aaactgaatc tgcattggtt aatgatttag aggaattctt acgtgctttc
    12361 aaagatcgaa ttggaaatta caaatatttt gatagaatta ataacttgat ggcggcgaat
    12421 tccacctcac ttgttgttga ttatatcgat tttgatactt ttaagccaga cttggcaaaa
    12481 ctaattacta acaaacccga tgaaatgttt gatgcattca atagagcaat atttaatgtt
    12541 ctctatgaaa tccatccaga ttatgcggat gagattcaaa ataacataaa ggtcagaata
    12601 ggaaattata cagtacaaaa aagtttacgt gaaataaatg cagaagtgat caataaactt
    12661 gttggagttt cgggaatggt tgtaagatca tctgaagtaa aaccattagc aaaaaaaatt
    12721 ggttatagat gtcttagttg tggtgaaata acagaagctc aactgcgagg attagtatta
    12781 aaaaaaccac taagatgtgg taaatgttct gaaaaagaac tagaaatgga tccagatacc
    12841 agcattttca ttgattttca aatggtaagg ttacaagaac ttccagaaga tcttcctgct
    12901 ggtcacttgc cacattattt agaagtaact attataggag atcttgtaga tcaatgtaga
    12961 ccaggagaca ggatcttatt aactggaatt gtaagaatag aacaagaaca attatcacaa
    13021 ctaatcaaaa caagtctttt tagattacga atggaaggaa ataatattga atatcttggt
    13081 ggaaggactg cagataaaga tagtaggaca atagaacgtt tggaaataag taatgaagat
    13141 gagaaacata tttttgcatt agcacgtaaa aacgatgcat atgatattct aatttcatca
    13201 tatgctcctc atgtatatgg acatgaagta attaaagaag taattttatt attaatagta
    13261 ggatcagtaa caaaaagatt agaagatgga tctgctagaa ggggagatat taatgttctt
    13321 ttagtaggtg acccgggcac agcaaaatca gaaatgttaa aatttgcggc caaaattgct
    13381 ccaagaggat tatatacttc aggaagaggt tctactgctg caggtttgac tgcagcagta
    13441 ataagagata aatcgggtat tatgatgttg gaagcaggtg ctgtagtatt aggggaccaa
    13501 ggtctagttt gtatagatga gtttgacaaa ataaaaccag aagatcgttc agctttacat
    13561 gaggttatgg agcagcaaac ctgttctgtg gcaaaaggag gaatagttgc tacattaaat
    13621 gcaagaactt ctatattggc agctgcaaat ccattatatg gcaaatatga tccttttaaa
    13681 aatattacag aaaatgttaa cttaccaata ccattattaa caagatttga tattatttat
    13741 gtaattagag atataccaga aaaggaaaaa gatcatcgca ttgctagtca catattagaa
    13801 ttacatagag aatcggaaag tactggacaa tatcttattg atattgatct ctttagtaaa
    13861 tatcttgctt attcaaaaac tatggaacct aaattaactg ttgaagctat agataagata
    13921 agagattatt atatgaaaat gagaaatgtc gaatcagagg gtatgattac tgttacacca
    13981 aggcaacttg aaggattagt cagattagca accgcccgtt caaaattatt attaaaagat
    14041 tatgtagatt cggaagatgc
//
LOCUS       DASVEV010000305         7943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_364933, whole genome shotgun sequence.
ACCESSION   DASVEV010000305 DASVEV010000000
VERSION     DASVEV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364933"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VF222_13275"
     CDS             <1..790
                     /locus_tag="VF222_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7143441.1"
                     /translation="SAMIVSSDVAFPPGETVPYLSLGQTVQVSYTFVDPITGNRESET
                     KSFVIRAIIKQSGNPNIDKAVIINEQIGNTLLHKSGKFDALIVVALSADNIDSIETEI
                     RKLYGNDIGITTVKALIQTIKEFTSGLSAFLLSIAIVSLIVGAVGIITTLYTSVIERT
                     REIGTLKAIGARNFDILTLFLVEALLIGVLGATSGLGTGVILGYILNTGFSSNSDGNN
                     SAPVFLITDMARVWLITVGLSFLAGLFPAFKASRLLPIEALKRE"
     gene            complement(1043..1801)
                     /locus_tag="VF222_13280"
     CDS             complement(1043..1801)
                     /locus_tag="VF222_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX7143442.1"
                     /translation="MNFRWLMNNSGGKSFVPSLCTTLLFIGFVILLSFSFLSFNNQFF
                     LNAQARSDDDCSSRDSNIIIGTRPITLGTKCNDVLIACPVIVSGSGLGAGCEIGDTVR
                     GFEKNDVIQGSVGNDELYGDEGNDKILGSLGSDKIYGGPDSDILQAGEGSDLIVGGNG
                     DDELYGGVGDDVSIGGKGVNFFDCGDGNDIIIDFDPNKGDTQTGNCEVILNNHGDMNI
                     FTQPNKIQSQLEAFGIGSSKDEIDINDLGKAIEN"
     gene            2247..2504
                     /locus_tag="VF222_13285"
     CDS             2247..2504
                     /locus_tag="VF222_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143443.1"
                     /translation="MKQYHKRFYFYGKSVPELLDKINEFTKEYSVGNIFDVSIMEVLD
                     KQIETDEKKYDNDGRVDDSDRIKISTFEYSYYAIVLILIEE"
     gene            2981..3256
                     /locus_tag="VF222_13290"
     CDS             2981..3256
                     /locus_tag="VF222_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143444.1"
                     /translation="MGSVFGPKDFGSGYKKIIDNSMKDLHPDTKDDVYKLLNTWEEIS
                     SEEKLRRIIGPERMEKLFNEVRKNIGNLNEDEKEDIKNIFKDSLTFD"
     gene            4874..5740
                     /gene="tfb"
                     /locus_tag="VF222_13295"
     CDS             4874..5740
                     /gene="tfb"
                     /locus_tag="VF222_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX7143445.1"
                     /translation="MITDVESGEIICNQCGIVVVKDLEDTTKIWHKVEDDKIDTMNGN
                     PSSLTLYDQGLTTRIGNTNRDASGNVINSMMVTRLNKMRYQDKRSQINISARYLIRAF
                     RQLNSLKDKMNLPHAVIEKTAYIYRKVQEAGLVRGRKVNTVLGASLYIACREFEIPRT
                     LREISSINNEKYRETSRVYRQIVLHLGKQVPQIDLFRYLEKVGKKAKLDEKNIRDALK
                     LMKKVKDAGLSAGKEPMGIVGAVIYLSLPKSNEHIRKRTITQAVIADAAGVSEVTIRN
                     VYKEIEKKLSLG"
     gene            complement(6300..6770)
                     /locus_tag="VF222_13300"
     CDS             complement(6300..6770)
                     /locus_tag="VF222_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7143446.1"
                     /translation="MSNGTNNIPADAQSASSSSKNEIIRGLNGDLAREYKAIIQYVVF
                     SSTLKGAEYGEIAQQLKEHASQELAHALEVAKQIDYLGGDPTMKGEEAEYSQDSKTML
                     EIDLRAEQETIKNYRERIRQAELAGEFALSETLREIIQQEQDHEIDLKDALGLR"
     gene            7064..7471
                     /locus_tag="VF222_13305"
     CDS             7064..7471
                     /locus_tag="VF222_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143447.1"
                     /translation="MSESDHVSDQTKFLYAFTKSILNSDRSIRWVGITDQNGIILNER
                     YRERLKLMLTIEETHEFAINTIMRHKTRLKFEPKIGELTYTFRRYKKMSRCLIPINES
                     YYLLLTMDFDQYDFDKIIMEKIIPIIKEKKENF"
BASE COUNT         2922 a         1083 c         1153 g         2785 t
ORIGIN      
        1 ttctgcaatg atcgtttcca gcgatgtagc attcccacct ggagaaacag taccttattt
       61 atcattggga caaactgtac aagtatctta tacttttgtg gatccaataa caggaaatag
      121 agagtctgaa acaaaaagtt ttgttattag agcaataata aagcaatcag gaaatcctaa
      181 cattgacaaa gctgtaatta ttaatgaaca aataggtaac acactccttc ataagtctgg
      241 gaaatttgat gcgttaatag tagtagccct ctctgctgat aatatagata gtattgagac
      301 tgaaataagg aaactttatg gtaatgatat aggcattact acagtaaaag ctcttattca
      361 gacaattaaa gagtttacca gtggattaag tgcttttctt ttaagtattg caatagtatc
      421 attaatagta ggagcggttg gaattataac aactctatat acttcagtaa tagaaagaac
      481 tcgtgaaata ggaactttga aagctatcgg agcgcgaaat tttgatattt taactctttt
      541 tttagtagaa gcgttactga ttggtgtttt gggtgccacc tctggtttgg gaactggtgt
      601 aattctagga tatattctaa atacaggatt cagttctaat agtgatggaa ataactctgc
      661 gcctgttttt ttaataactg atatggcgag agtttggctt attactgttg gattgagttt
      721 tttagctggg ctgtttcccg cgtttaaagc atcacgttta ctgcccatag aagcgttaaa
      781 gagggaataa gatcaaaaaa acattttttc caaaacaata ttatttaata tgctgatacc
      841 ttcgatgagg aaaagtaggt agttttaata ttcaatgaat acccgatatt atggataaaa
      901 taatttgaat tgttattgta caaatagaac gacataaaga tattttgatt tatatagcat
      961 tattttgccc aaattataaa ctagtataca cgaaactaca tgataaataa ttgagttttt
     1021 actattgaaa ttgtattagt cattaatttt caatagcttt tcccaaatcg ttgatatcaa
     1081 tttcatcctt tgaggatcca atgccaaatg cctcaagttg agattgaatt ttatttggtt
     1141 gggtaaagat attcatgtca ccatgattgt ttaagattac ttcacaatta cctgtttgag
     1201 tatcaccctt attgggatca aaatctatta taatatcatt accgtcacca caatcaaaaa
     1261 agtttacgcc ctttcctcct attgaaacgt catcacctac acctccataa agttcgtcat
     1321 ctccattacc tcctactatt aagtcagatc cctcaccagc ttgaagtatg tcgctatctg
     1381 gcccaccata tattttgtcg cttcccagtg aacctagaat tttatcattt ccttcgtcac
     1441 cgtaaagttc gtcattacct acagaacctt gaataacatc atttttttca aatcctctaa
     1501 cggtgtctcc aatttcacat cctgcaccca aacctgatcc actcacgatt actggacatg
     1561 caatgagtac atcgttacat ttggttccca atgtaatagg acgggttcct attataatat
     1621 ttgaatctct tgaactacaa tcgtcatcac ttcttgcttg agcatttaag aaaaattgat
     1681 tattaaatga taaaaacgaa aatgacaaca atattacaaa tccaatgaac aataaagtag
     1741 tacagagtga tggaacaaaa cttttacctc cagaattatt cattaaccat ctgaagttca
     1801 tgataataat ataatctcct tactaataaa taaaattttt ttaaatatat aattaaattc
     1861 tataaaaaaa tttgaaattt ttttctacta ttcggtatca ctgatttgta tcatcttgaa
     1921 aaaaatgaga gaatatgaaa ctacatattt tgttaataat taaagtaaag aatataataa
     1981 aaatcattaa ttaatcaaac aaaataatga ataataatta tttggatatt aatattagta
     2041 aaaaggtgtc taagaattgt aaatataatt gtctataatt gatattaact tgaatgcaat
     2101 gaattgtatg aaatagcaaa tattgaaaat tagatattaa ctataatgtt ttctacaatt
     2161 ttagttatat ttaaatattc aatctattat aatttattga tttcctgttg caaatagtaa
     2221 tatatacaaa catctattaa aattaagtga aacaatatca caaaagattt tatttctatg
     2281 ggaagtctgt tccagaatta ttagataaaa taaatgaatt taccaaagaa tatagtgttg
     2341 gaaatatctt tgatgtatca ataatggaag tattagacaa gcaaatagaa acagatgaaa
     2401 aaaaatatga taatgatggt agagtcgatg attctgatag aataaagatt agtacttttg
     2461 aatattcata ttatgcaatt gttcttattt tgatagagga ataaatgaat aactctaaat
     2521 tgataccaaa gttattaaca aaaacaattt tttcagaatc tcaatctaat tttataaata
     2581 aagtttgagt tcttaatcgt ctttgttaat tctgtaagct caatctgctt cattctcctt
     2641 ttgcattcca tttaaataaa aatattttct gccaaatcag taaaaaaatt accttttttc
     2701 atataatata cacatcatag ccgttcattt agaagaccat gaactgatta taaaataaat
     2761 ccagtaaacc aatttaaata aaatatgtaa taaaccatca aactattaat cttttttaaa
     2821 atgccttttt tcttttcggt tattttaaac caaatgtcat agtcatcata acaatcgaag
     2881 agcttttgag gattgttgtg taatagttat tgatttatgg ataatgaaat tatctctcta
     2941 gatccaatac acttattatt taaaattaat ataaagtatt atgggatcag tatttggacc
     3001 caaagatttt ggaagcggtt acaaaaagat tattgataat tcaatgaagg atctacatcc
     3061 tgatactaaa gatgatgtct ataaattatt gaatacatgg gaagagataa gttcagaaga
     3121 aaaacttagg agaataattg gccctgaaag aatggagaag ttatttaatg aggttagaaa
     3181 aaatataggt aatttaaacg aggatgagaa agaggatata aaaaatattt tcaaggattc
     3241 gttaaccttt gattaaggaa cttcttgatc tttatactta accgcagata aaagaccaga
     3301 cattcatctt atggagttgt gttttaaata tttcctactt catgaattct ttatttttct
     3361 atatgttctt gaatattaat ttaattgaga ttacttaagg actactatta acagttattg
     3421 ttagactaca atatctttaa ttactcctat tagtaacaag atctacaaaa taaggagtga
     3481 attgtgatta aatattgaaa caaactgata taatgattaa gtttatattc ttttagacta
     3541 gtcataatat taatatataa aaaatatgta atagacagta aaatttaatc ctaaatagat
     3601 actttgcttt tgtcccttag ttctgtcatg atactttgta tattctagtc ttcttgataa
     3661 tttaaataat tccaccagta aacataatta tgcaaaaaga cttataaaaa aaaacaaaga
     3721 aaaattatag taaataatgt ctgtcttatg gggatggttt tttgaatgaa ttgttcctga
     3781 tagatatgag ataattggct cattaattgc agtttttgga gcgattataa tgttttattc
     3841 tccgagataa agtagaaaat tattttactt tctcctatga cgttattatt aagttctatg
     3901 tccttgaaat tgatgattat tactttgaaa tgtgttgatt gttattacga tgttggtgag
     3961 tgtaaggtaa gtctatctcc ttttgaatac cagtgttgta ttcttggtaa atgttgttgt
     4021 tgggcaagtt ttcacgttta aaaatttgtt ataatcgaga tcttacctaa ccgttgattt
     4081 ttattctctt ctgagatgct aataactcca ttagtaatta ttaaaaaatg agaaagagta
     4141 ttttatatat acgatttttg tgttatatga attaatatta ataattagac ttaaacaatt
     4201 ctaaacggta ttttagttta cttgaataca tatatcaata aattctatat gtaactcgta
     4261 atactattcc gttgaatcaa cagagagggt aaaccaataa tgcatttcaa gaaaaagaga
     4321 ttttcatccg aatttataag cctctctcaa ttttagagtt tctattaaat tctcaaaata
     4381 tacatctatc aaaatataat attataataa tctacctctt agaaagaaag agaccagata
     4441 gaataaacct tttaatttaa ctgctagaaa gtaatggata taaaaaaact acatcttcaa
     4501 ctttgaacgt tttaccaaat aactaatata ggaaaagaag ccgattcaag ttggataaca
     4561 cattatcgag aatatgcaaa gatgctccta acacatttga tctattacac aaacacaaat
     4621 cgaggaagta gaacaacgat tataaaaaac aggagatatt ttatttctac ttaatttaat
     4681 aaccctttac taaattaata caatgatagc taaatctatt tggttaaatt ataatcgatg
     4741 ttagtcttta tgtaaatttt ataatgataa gtataagatt tatatatttg tttagctata
     4801 attataataa atacaaataa aaaatatgca aaccacaatt caatgtaaaa tatgtgatcc
     4861 tagtaaatcg aaaatgatta ctgatgttga atctggagaa attatttgca atcaatgtgg
     4921 aatagttgta gtaaaagatt tagaagatac aacaaagata tggcataaag ttgaagatga
     4981 taaaattgat acaatgaatg gtaatccttc ttctttaaca ctctacgatc aaggtcttac
     5041 tacaagaata ggcaatacaa acagggacgc tagtgggaat gtcatcaatt ctatgatggt
     5101 aacacgtcta aataaaatga gatatcaaga taaaagatct caaattaaca tttctgctcg
     5161 atatttaatt agagcattta gacaattaaa ttcattaaaa gataaaatga acttaccaca
     5221 tgctgttata gaaaagacag catatatcta tagaaaagta caggaagctg gattagtcag
     5281 aggtagaaaa gtaaatacag tattaggcgc gtccttatat attgcatgtc gagaatttga
     5341 aataccaaga acattaagag aaatttcttc aattaacaat gaaaaatatc gagagacctc
     5401 ccgagtgtac cgtcaaatag ttttgcatct cggcaaacaa gtaccacaaa tagacttgtt
     5461 tcgctaccta gaaaaagtag gaaaaaaagc aaaacttgat gaaaaaaata tacgtgatgc
     5521 attaaaactg atgaaaaaag ttaaggatgc cggattgagt gcaggaaaag aacctatggg
     5581 catagttgga gccgttatat acctatcact ccctaaaagt aacgagcaca tcagaaagag
     5641 aactattaca caagcagtaa ttgcagatgc agcaggagta tcagaggtta cgattagaaa
     5701 tgtatacaaa gaaattgaaa aaaaactttc gctaggataa tttaccaaat tttttatttt
     5761 aaataaatat tgctaaacct ttttttatta tttataaact ataactaaca caagattcaa
     5821 atattaaaaa atctttggcg aaaaaattta gttaattatg tatctattag ttatcttacc
     5881 tacttcagca ccaacattat tacattgtat aagttataaa tcaatattgg taagatattt
     5941 atactaattg aaggataact tagtataatc attcaatgta gaacctactc cacctagaat
     6001 gtaaaatttt actgcgaaaa atatctgtaa aagtaatggg gatatttgta tattgtaagg
     6061 ttacaaaaaa cgacatattt gtataatagg aaaataacca aatgcctaat caataatttt
     6121 tgtattatac atattcaaaa gtgattataa atcgttagta tatctatttt tatcaattcc
     6181 tatttttgtt tctttacctg ttttaacatt cctataaata gatttattaa tatcattgaa
     6241 aaatagttat gagtttatag attactgaga ttatctcagt atgatataga gatttgctat
     6301 catcgcagtc ctagggcgtc ttttagatct atttcgtgat cttgttcttg ttggataatt
     6361 tctcttaatg tttcagagag agcaaattca ccagcgagtt cagcctgacg gattctttct
     6421 ctatagtttt taattgtctc ttgttctgct cttaggtcta tctcaagcat tgtctttgaa
     6481 tcctgggaat actctgcttc ttcccctttc attgtcgggt ctccacccag atagtcgatc
     6541 tgcttagcca cctctagagc atgtgctagt tcctgtgatg catgttcttt caattgttgg
     6601 gcaatctcgc catactcagc gcctttcaat gtagaactga atacaacata ctggataatt
     6661 gctttgtact ctcttgccaa gtctccattt agtcccctga ttatctcgtt cttgctagaa
     6721 gatgatgcgg attgagcatc tgcaggtata ttattagtac catttgacat atatttacat
     6781 taaatccatt tctcttaaat gttattgagg taatgaataa tctttacttt ttgttcttta
     6841 tgataaagac aaaactaaaa aaaatctagt ggttggaata agatttttct atcttattta
     6901 caaagttaag gtagttttga gtgctgcttc attataatag ttttgtgcaa tttcaattat
     6961 atgagaaata atttagaaga gaattaaatt ttatttctta tgcagtaagt tttagattaa
     7021 ataacttttt tattacaatc taaaattact tactaatata catatgtcgg aaagcgatca
     7081 tgtaagtgat caaactaaat tcctttatgc ttttactaaa agtatattga attctgatag
     7141 gtccataaga tgggtcggaa taactgatca aaacggtatt attctaaatg aacgatatag
     7201 agaacgacta aaactcatgc ttactataga ggaaactcat gaatttgcaa ttaatacaat
     7261 aatgagacat aaaacgagat taaaattcga accaaaaata ggtgaattaa cttatacgtt
     7321 tagaaggtat aaaaaaatga gtaggtgttt gattccaatc aatgaaagtt attatttact
     7381 tttaactatg gattttgatc aatatgattt tgataaaata ataatggaaa aaattattcc
     7441 tataattaaa gagaaaaaag agaatttcta aacaggcatt taattttatt tcttattttt
     7501 aaaatattta ttctaaacat gaacagaaac cttgtgcttc gactgataaa taatttgatt
     7561 catatcttat caataaggtc acaaaacaat aatgaaaatg tatacaattg tcaatgcaac
     7621 aataaaccag attgttatta ttatttgaat ctaagactcg tataacatgt cctctagcct
     7681 ggtttttcag tccaaagaac tccaaacaga acctattata ataatagaca acattgttaa
     7741 ttaagttaaa gttaattttt tgttatgtag ttattttttt tgcttttttt ataattaaaa
     7801 caaaaactaa aaaaaatcta gtggttgtaa taagattttt ctatcttatt tagaaagtta
     7861 aagtaatttt gaactgcttc attataatat ttttgtgcaa tttcaagtga tttgttaaag
     7921 gtttctgcag atgctaatgc aat
//
LOCUS       DASVEV010000306         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1139772, whole genome shotgun sequence.
ACCESSION   DASVEV010000306 DASVEV010000000
VERSION     DASVEV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139772"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..296
                     /locus_tag="VF222_13310"
     CDS             135..296
                     /locus_tag="VF222_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143448.1"
                     /translation="MDNIVINIIGQSALAWYIEIASDEHKNIMNGFITIRALKFIKEN
                     NIFINILNL"
     gene            691..2193
                     /locus_tag="VF222_13315"
     CDS             691..2193
                     /locus_tag="VF222_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143449.1"
                     /translation="MKIVIIAIFFFLSFMVCLTFLYSDVYGFSPQLKTEFLSKEIQNY
                     IYYGPDNSSNKTLKYLQQISDLDRVYVTSNGTHLEIKFLFNDFLNKLKNTLLNSSTVP
                     QNLFINIFVDSDDDETTGFLGYNYRYLLTHNNANFYKNNTLNSTTNFSQNFKQNQSNH
                     NDILEGDELQEIITRTINSKELIEKLDWSIRGYEVLDYDFQPLFFSSNLTKKYLSVIP
                     DGFKVTLDLAQIGYPTNYAILVEVGRKSEDYKFSHVFGKIHVPRPDLILEDKSININN
                     GKNSIVLEFNNTGLYNLNVKTELLKKNLSDDDLSINFSQGNQFKLLSGKGVLPMDIIA
                     NSNYNQKSLIIPLNLSYSVIGENDFIESGFNNSLTNENIYNKILYLNLNLINERNRLI
                     NFDEIPSQYIAVFLGAVFSFFIPSITRSTKEYFQKRNANKDLKNILKEQNSDNPDTSI
                     KNLINKAKILKHEFIRGKITKEQYEILKENIADVVKDLVSKKTDTTTDKK"
     gene            complement(2338..3699)
                     /locus_tag="VF222_13320"
     CDS             complement(2338..3699)
                     /locus_tag="VF222_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7143450.1"
                     /translation="MTNNNPLPPGPQITIQDFMTNVKENPFDLLMDLIQKYGDIIYLK
                     LQKDVYILSNPDYIEQIFTRDYKLFSKARAAELRPFLGNGLLLNEGESHRQHRKIIQP
                     LFLPKSIKSYSNIMVDNIKHISDNWQDNTTIDVLQEMTMLAMGVMTESLFGINFRDRD
                     TFSKVSNSFTNILESFTQVHEPVKNILVEESTNENKKQNKFQDSLDYLNQKIYTLMDH
                     IKKTNPEKPNLISHLINAKDPDSGEIGLPEKQIRDEILIFLFAAHDTTSTALTWSLAY
                     LATNQEIQDKLQKELDSVLEGGRVPTGDDLPKLEYAEKIFKETLRIRPSVWALSRLTN
                     EEYKIGEYVIPNSSVIFMSQYAMHNSPKYYIDPDIFNPERWTKEFLFKLPRFAYFPFG
                     GGIRSCIGETFAVQEGILALATIFQKWRIVPTTEGISFEPKALGGFTKPKYPINVIVK
                     SRQ"
     gene            complement(3794..>4095)
                     /locus_tag="VF222_13325"
     CDS             complement(3794..>4095)
                     /locus_tag="VF222_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143451.1"
                     /translation="INEVKSQLNFTSVDSHLSSASQNFINGKISEGLSELKSANEEWK
                     NSSMTIIKIGSDFISMAKNNSTSTNDSTREILDKFGNILVNIGKKIENLRIQLSD"
BASE COUNT         1484 a          584 c          550 g         1477 t
ORIGIN      
        1 ttattacaaa attatttgtt ggtaatgact gacaataaag atattacttt ctattatgat
       61 agattcgaga ttattatata atttttcata ccaaaaaatg gaacaaaatt gatatagata
      121 aatttaaaga cgaattggat aatatagtta taaatattat tggccaatct gcattagcat
      181 ggtatatcga gatagcatcc gatgaacata agaatataat gaatgggttt attacaataa
      241 gagcgttaaa atttattaag gaaaataata tttttataaa cattctgaat ctctgaatag
      301 tttaaaaccc atagaaccta tattgctagc atgtaggtcc aggcattcca tcaaatataa
      361 aggatatact ctttactaaa tttgtaacag gctcaaaatc aggaactggt ttaggtctct
      421 acatttgcaa aaatattata gaaaaacatg gtggaaaaat atgggcggag aataataaga
      481 acaaaggtgc aaattttaat tttattatgc ctattacatc taagcagtaa aatttatttc
      541 aaatgttaaa taataagcat ctttattgcc tacctgtcag actcattaga atttgcatta
      601 aaccattact agagtcataa tgcacatttt agataaatag attagaaagg atgaaaaaat
      661 taaaattgga aagtacttga aagttcacta ttgaaaattg taattattgc tatttttttc
      721 tttctttctt ttatggtttg tcttactttt ttatattctg atgtatatgg attttctcca
      781 caattaaaaa ccgaattcct aagtaaggaa atacaaaatt acatatatta tggcccagat
      841 aacagttcca ataaaactct aaaatattta caacaaatta gtgatttaga cagagtatat
      901 gtaacaagta atggcactca tcttgaaata aaatttttat ttaatgattt tctaaataaa
      961 cttaaaaaca cgttattaaa tagtagcact gttccacaga atctttttat taatatattt
     1021 gtagactctg atgatgacga aacaactgga ttcttaggct ataattatag gtatttgtta
     1081 acccataaca atgcaaattt ttacaaaaat aacacattaa attctactac aaacttttct
     1141 caaaatttta aacaaaatca atcaaatcat aatgacattc tggaaggtga tgaactacag
     1201 gaaataataa caagaacaat aaattctaaa gagttaattg aaaaacttga ctggagtata
     1261 cgaggatatg aagtcttaga ctatgacttt caaccgctat tcttttcctc caatctcact
     1321 aaaaaatatt tatcagttat tccagatggg tttaaggtta ctttggacct tgctcaaata
     1381 ggttatccta caaattacgc aatattagtt gaagttggaa ggaaatcaga agattacaag
     1441 ttttcccatg tttttggaaa aattcatgtt ccacgtccag atttgatttt agaagataaa
     1501 tcaataaaca tcaataatgg caaaaattcg atagttctag aatttaataa tactggttta
     1561 tacaatttaa atgtgaaaac tgaactacta aaaaaaaatc tttctgatga tgatttatca
     1621 attaatttta gtcaaggaaa tcaatttaaa ctattaagcg gaaaaggtgt actccctatg
     1681 gacattattg ctaattctaa ttataaccaa aaaagtctta ttattccact caatctttct
     1741 tatagtgtaa taggtgaaaa tgattttatc gaatctggtt ttaataattc actaaccaac
     1801 gaaaatatat acaataaaat actttacttg aaccttaacc ttattaatga aaggaatcgt
     1861 ttaattaact ttgatgaaat tccctcccaa tatattgcag tatttcttgg agctgtattc
     1921 agctttttca tcccatctat tactaggtca accaaggaat attttcaaaa acgaaatgcc
     1981 aataaagatc taaaaaatat tcttaaggaa cagaattcag ataatcctga tacttcaatt
     2041 aaaaatttaa taaataaagc caaaattcta aaacatgaat ttattagagg aaagataact
     2101 aaagaacaat atgaaatatt aaaagaaaac atagcggatg ttgtaaaaga tttagtatct
     2161 aaaaaaactg atactactac cgataaaaag taacaaaaaa agacatgatt atggtaatac
     2221 aataccctaa taaattttaa tatctcaaaa cagagttttt agataatact tttaatcatt
     2281 ctggatattc aaatggtgaa atctttactg ttgcatatat taaaatacaa ttataaatta
     2341 ctgtctgctc ttcactatta cgtttatagg atatttcggt ttggtgaatc cacctaatgc
     2401 ttttggttca aaacttatcc cttccgtggt aggcacaatt ctccattttt gaaagatagt
     2461 tgctaaggca agaattcctt cttgaactgc aaatgtttct ccaatacatg acctaattcc
     2521 tccaccgaat gggaaatagg caaatcttgg caacttgaat aaaaattctt ttgtccatct
     2581 ttcagggtta aaaatatctg gatctatata gtattttgga ctattatgca ttgcatattg
     2641 actcataaaa ataacagaac tgtttggtat aacatattct ccaattttgt attcttcatt
     2701 tgtcaatctt gataatgccc atactgatgg tcttattcta agggtctctt tgaaaatttt
     2761 ttcagcatac tctaactttg gtaaatcatc accagttggt actctccctc cttcaagtac
     2821 tgagtctaat tctttctgta gtttatcttg aatctcttga tttgttgcaa ggtaagccaa
     2881 tgaccatgtt aatgcagttg atgtggtatc atgagcagca aatagaaata tcagtatttc
     2941 atctctaatc tgtttttcag gtaatcctat ttcaccactg tcaggatctt ttgcgttaat
     3001 gagatgtgag ataagatttg gtttttcagg attagttttt ttaatatgat ccattagagt
     3061 ataaattttt tgatttaaat aatcaagaga atcttggaat ttattttgtt ttttattctc
     3121 atttgttgat tcctcgacaa gaatgttctt gacaggttca tgtacttgag taaatgattc
     3181 taaaatattt gtaaatgaat tgctaacttt tgaaaatgta tccctatctc tgaaatttat
     3241 tccaaataaa gattcagtca ttacacccat agccaacata gtcatctctt gtaatacatc
     3301 aattgtagta ttatcttgcc aattatcaga aatgtgtttt atattatcaa ccatgatatt
     3361 gctatatgac ttgattgatt taggtaaaaa taatggttga ataattttcc tatgctgtct
     3421 atgtgactct ccttcattta acaatagacc attccctaaa aaaggtctta gctctgccgc
     3481 tcttgctttg gaaaataatt tataatcacg agtaaatatt tgttcaatat aatccggatt
     3541 acttaaaatg taaacatctt tttgaagttt caagtatata atatccccat acttttgtat
     3601 taggtccatt aacaaatcga aaggattttc tttaacatta gtcataaaat cttgaatagt
     3661 tatttgtggt ccaggtggaa gaggattatt atttgtcaat aagactgaaa tttcatatta
     3721 ctttataaga attacttttt taaaatctaa tattttttaa aatgtattgt gattgtactt
     3781 atgtaaatac atgtcaatca gaaagttgaa ttcttaaatt ttcaattttc ttaccaatgt
     3841 ttactaaaat attaccgaat ttatctaaaa tctctctagt actatcattc gtagatgtcg
     3901 aattattttt agccatagaa ataaaatcgc ttccaatttt aataattgtc atactagaat
     3961 tcttccattc ttcatttgca gattttaatt cagataatcc ttcagatatt ttcccattta
     4021 tgaaattttg agaggctgat gaaagatgtg aatcaacaga agtaaaattt agttgtgatt
     4081 taacttcatt aatta
//
LOCUS       DASVEV010000307         8704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1140334, whole genome shotgun sequence.
ACCESSION   DASVEV010000307 DASVEV010000000
VERSION     DASVEV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140334"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF222_13330"
     CDS             complement(<1..208)
                     /locus_tag="VF222_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143452.1"
                     /translation="MSNNPNIKENSINSPSSFSFKCVESGGLANNYLVISFDSDSNNL
                     KVSADISGANLTQKSLEDSEKNDLI"
     gene            complement(442..711)
                     /locus_tag="VF222_13335"
     CDS             complement(442..711)
                     /locus_tag="VF222_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7143453.1"
                     /translation="MRNRSKTEIVAMILDAANDGGETKTRIMYFAFLSYNQVKEYLSV
                     LIENNLIEYLDGANKYKTTEKGLNYLKMHNEIGELLQQTTTIKNN"
     gene            1183..2187
                     /locus_tag="VF222_13340"
     CDS             1183..2187
                     /locus_tag="VF222_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143454.1"
                     /translation="MEGDINNITYNEKNNNNNNITLCSICKNDFQIVSDPESGEIICE
                     ICGIVLSEEKKLPLHTRVYEEMNANSHFNTTTNTNSQNHDNNSNNNNILLNIESSTII
                     GRSNVDAIGKKISNDMQAKVDRLRVWEQRTRYTNPRDRNLKHAFNKLNNIKDKMVLSS
                     AIVEKSFYLFKKASSQGIIRGRSTEGVLSASVYLACKELETPKTLKEISLITNVKIKN
                     ISKYSKVLIMKLGLKPIPVIDPIKCIVKIANNLDLDEKSKHKAIKIMKEIMKEEIHVG
                     KNPLSMAASTIYAVCKTVEGEDITQSEIANSVGISALVIRDRYNDIIDKINLDQIIYT
                     "
     gene            2657..3859
                     /locus_tag="VF222_13345"
     CDS             2657..3859
                     /locus_tag="VF222_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7143455.1"
                     /translation="MKKVCVLGSGYAGLFSAANLLADNDFKNEFEINIFDKNPYHQLL
                     QQIHLVTAGIKKTREISFSIYDLMKDDVTFYNEVVLGVNFNENKIITSNNKEYDFDYV
                     IIALGASNAFFGIKGAKEYAQSFRSLNDALVLQNKIRNINEDIKIIICGGGATGVSLA
                     GALCETFKEKIKITIVEAQSDILPEWNPKLVRSIKNFLKQNNVEIITNNPITEVRQSS
                     VLLNDNTILENTLSIWTAGIKGQEIHTIPEIEKTKSNRIKVNNFSQVEGFNNAFALGD
                     ISAFPIDNLHLSPQLAQFAVRQAMNVAKNIIRREKGKEMVRFHFEQHGSILSLGRKCI
                     GIMNGVIIKGSLCGYVEDFLIDNYIATIKNRGRGISSLAYEQHKLSQISSSLSFIIST
                     ASKILSSD"
     gene            complement(4499..5221)
                     /locus_tag="VF222_13350"
     CDS             complement(4499..5221)
                     /locus_tag="VF222_13350"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7143456.1"
                     /translation="MTYILDNHKVVSESEWIEARKELLKKEKEFTILRDQISQQRRDL
                     PWVQVNKEYVFDGPNGKQTLSELFDGRSQLIVYHLMYDPTWDAACPSCSFWADNFNDI
                     FVHLNQRDVTMIAISKAPYSKIDAYKKRMGWDFKWVSSFDTDFNRDYYVSFTSEELEK
                     KEGFYNFTIQNPYVSELPGVSVFYKDPSGKIFHTYSAYARGIDILNNTYNYLDLVPKG
                     RDEDGYDFPMAWVRRHDEYNKK"
     gene            5400..5720
                     /locus_tag="VF222_13355"
     CDS             5400..5720
                     /locus_tag="VF222_13355"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7143457.1"
                     /translation="MTGSVWKALAEDNRRQILLLLREHDMFPSELAKHFDTTFAAVST
                     NLRILKDADLIKETREGQKKRYSINPKKTLEIKEFFDKMWGHNLEGLKEFVESKEEKK
                     KNER"
     gene            5710..6246
                     /locus_tag="VF222_13360"
     CDS             5710..6246
                     /locus_tag="VF222_13360"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7143458.1"
                     /translation="MSDKYDVLKKEIVINAPVEKVYSALTTPEQLTQWFPNIVTIEPK
                     IGGKISFRFLKESTQEDKDHEIVGEIVSFIPNKELSYTWNFTTKREYNKNTIVTWKLE
                     QLDKDKTKLTLIHSGFTNADRLQYDDHNKGWSWHIKRLENLMDKNTNANEIVMQYLQA
                     VENRDFESARKYVSDNIS"
     gene            complement(6560..7090)
                     /locus_tag="VF222_13365"
     CDS             complement(6560..7090)
                     /locus_tag="VF222_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143459.1"
                     /translation="MPHISQEIHISKETWNGAKRLIKNGIIKKLSAAKQNIDIDIEIA
                     AGIYIYALEEFGKLLLLKESQTVDGKYIVKYRDGFRSHDFKFNKAFDYLQNDGYGRCI
                     ILNDDGAFTPDAFSWRSFTIGLVAQTEARLGIFYVDFTKSENDNYEIMEIPTVDQNKL
                     NEAINKLEEVIDTFEL"
     gene            7568..7816
                     /locus_tag="VF222_13370"
     CDS             7568..7816
                     /locus_tag="VF222_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143460.1"
                     /translation="MTIRAIEVWDAADRSESLYQRINDVLRDNNITREELIDIKYSTV
                     YIPEDKKITSQALIIFDKKEEIVMTELDVVDDKDVLRD"
     gene            8101..8385
                     /locus_tag="VF222_13375"
     CDS             8101..8385
                     /locus_tag="VF222_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143461.1"
                     /translation="MKDCSGAGDLSNIASTYIGIIIGALLGGLISWLIYNRQEKISKT
                     QDTMLSGIKKLQDKQEHILEKIQTFEQNHDIILNDIFMLEKKIDSLLQRK"
BASE COUNT         3282 a         1201 c         1294 g         2927 t
ORIGIN      
        1 ctattaaatc atttttttct gaatcttcta aagatttttg ggttaaatta gcaccactaa
       61 tatctgctga tactttaagg ttattagaat cggaatcaaa agaaataact aaataattgt
      121 ttgccaatcc tccagattct acacatttaa agctaaaaga cgaaggacta tttattgaat
      181 tttctttaat attaggatta ttagacatgg attctcaata gcataacatt aacatcttat
      241 ttaaataatt caattttata ttggatttca tactaacgag tataaagact tttaataacg
      301 tatttgaaac atttaataga aaacttatat tttttcttac taccttgtgt aaaaataatt
      361 tggtatattg taaaaagaaa aaagatacat tttctaattc acaggtagat gttttattat
      421 atcaaaaaaa tattaaatta attagttatt tttaattgta gtagtttgtt gtaataattc
      481 tccaatttcg ttatgcattt taagatagtt tagtcctttt tctgtagttt tatatttatt
      541 agccccatcc agatattcta ttagattgtt ttctatcaaa acagaaaggt attcttttac
      601 ttggttataa cttagaaaag caaagtacat aattcttgtc tttgtttctc ctccatcgtt
      661 tgcagcatcc agtatcattg ctactatttc tgtcttactt ctatttctca tattattaga
      721 aatgtgattt actattttaa tattaatacc acaagaaaag ttaacctaac tatagtaaaa
      781 cattcaacat attatttaca aattaaaatt agaacggttc tatcattaca ttgtattagc
      841 ttttattatt agaacttatt ttttaaaatg ttttttggaa tattactata ggaattcatt
      901 agaaaagtta gaggaagtaa taccgatagc gatgcgaaaa taagagtcta ctgtgataga
      961 ataaggataa atatttaaaa tatctatcgt aaaaataatt cacaaaatat attccaatcc
     1021 cacatccaac aaagatgctt tgtctggagt tctgtttgta gtatggttct accacatttt
     1081 atttagcttt ttggaaatat atttaaaatt tttatttaat atagattaaa catatctttt
     1141 ttagtaataa atgtatatta tattttaagg gggttattat atttggaggg agatataaat
     1201 aatataacat ataatgaaaa gaataataat aataataata ttacattatg ctcaatttgt
     1261 aaaaatgatt ttcaaatagt atcggatcct gaatcaggtg aaataatttg tgaaatttgc
     1321 ggtatagtct tatctgaaga aaagaaatta cctttacata caagagtata tgaagagatg
     1381 aatgctaata gtcatttcaa tacaacaaca aatacaaatt cacagaatca tgataacaat
     1441 agtaataaca acaatatctt gttgaatatt gaatcttcta cgattattgg aagatccaat
     1501 gtagacgcta taggaaaaaa aattagtaat gatatgcagg ctaaagtaga tagattaaga
     1561 gtatgggagc aaagaactag gtacaccaat ccaagggata ggaacttaaa acatgcattt
     1621 aacaaattaa ataatatcaa agataaaatg gtactatcta gtgctatagt ggaaaaatct
     1681 ttttatctat ttaagaaagc atcatcacaa ggaataatac gaggaagatc aacggaagga
     1741 gtattatctg cctcagtata cttagcatgt aaagaacttg aaactccaaa gacattgaaa
     1801 gagatttcgc ttataactaa tgtaaaaatt aaaaatattt caaaatattc aaaggttctt
     1861 attatgaaac ttggtttaaa accaattccc gtcatagacc caataaaatg tattgtaaaa
     1921 attgcaaata atcttgattt agatgagaaa tcaaaacata aagccataaa aataatgaag
     1981 gaaatcatga aagaggaaat tcatgtggga aaaaatccat tatcaatggc tgcatcaaca
     2041 atatatgcgg tatgtaaaac tgtagaaggg gaagatataa ctcaatcaga aatagcaaat
     2101 tctgtaggaa tatctgcttt agttataaga gatagatata atgatattat tgataaaatt
     2161 aatttagatc aaataatata cacttaatca agtataatca aatcgtgact atttttccta
     2221 aaattgttaa atacgtcaac aggattagta tatgcatcta caatatagcc attgtactgt
     2281 aattgctatt atactaagta tcattggcat cgtcattatt gctaatatag aagtcttaat
     2341 catattactc gacaagaagt aaagtgatgg acctaatccc aaagcagccc aagctttaca
     2401 ggaaggtctt atattaataa gaataagaat attggtaaaa caattaattc tatattaaag
     2461 aatatcaatt gatttcataa tcagtactta aatttttcgg cttctaaaaa tttttcatca
     2521 aatttccttc gaataactta attattacag tgatatagaa aatgaatttt aagtaagaac
     2581 aatgtctata taaaaccaat ctataactag aaattgataa agttatttta ttaggtggat
     2641 attaaacaaa aagtgaatga aaaaagtgtg tgttttaggt tctggttatg ctggactatt
     2701 ttcagcagcg aatttattgg cagataatga ttttaagaat gaatttgaga taaatatttt
     2761 cgataaaaac ccttatcacc aacttttaca acaaatccat ttggttactg caggaataaa
     2821 aaaaacacgt gagatttctt tttctattta tgatttaatg aaagacgacg ttacatttta
     2881 taatgaggtt gtcctaggag taaatttcaa cgaaaataaa attataacat caaataacaa
     2941 agagtatgat tttgattatg ttataattgc attaggtgca tccaatgctt tctttggaat
     3001 taaaggagca aaggaatacg ctcaatcttt taggtcgtta aatgatgcat tagttttaca
     3061 aaataaaatt cgaaatataa atgaagatat taagattata atatgtgggg gtggagcaac
     3121 aggagttagt ttagcaggcg ctctctgtga aacatttaaa gaaaaaataa aaataactat
     3181 agttgaagct caatctgata ttctcccgga atggaatcca aaactagtta gatctattaa
     3241 aaatttttta aaacaaaata atgtagaaat aattacaaat aatccaatta cagaagttcg
     3301 tcagtcatct gttttattaa atgataacac aattttagag aataccttgt ccatttggac
     3361 tgctggaata aaaggtcaag aaattcatac aatacccgaa attgaaaaaa caaaatctaa
     3421 taggataaaa gtaaacaatt tttcacaggt cgaaggattt aacaatgcat tcgctctagg
     3481 cgatatttct gcatttccca ttgataattt acatttatct ccacaattag cacaatttgc
     3541 agtaagacaa gcaatgaacg ttgcaaagaa tataatacgg agagaaaagg ggaaggaaat
     3601 ggttagattt cattttgaac aacatggtag tattctttct ttaggaagaa aatgtatagg
     3661 aattatgaat ggtgtaatta taaagggatc tttatgtgga tatgtagaag actttcttat
     3721 agacaattat atagctacaa tcaaaaatag aggaagaggt atatcttcat tagcttatga
     3781 acaacataaa ttatcacaaa tatcctcatc acttagtttt attatcagta cagcttcaaa
     3841 aatattatcg tctgattaat gttttattta ctatcaaatt atagaaggta aattgaatta
     3901 aaattttatt tccactctaa caaactaaat tcttagttgt atatttcctt tttgcaatat
     3961 tcaatttgat atttattagt actacgataa caaaaatgag ccacacttac taggacaagg
     4021 ttagcagtta tggctctgat acttgttgcc aagtaaggta gtgatattag gacatcaacc
     4081 tatttctgat acaacagaat taataaaata actatccaat gaaaatataa aaaatgtata
     4141 tgtaggtgga ggagtaacta ttcaaaattt tctttcttgt aaactagctg atgaaattac
     4201 aataagaata tttccactat ttccaataat gcttggtgaa gacgaccttt atttggacat
     4261 ttggcaaatg aaattttatt atctcctatt gacgtgaatc gatttgttca acttaaatat
     4321 caagtcataa aatctgtgca ccaaaacttg ttataacctt ttgtaatttc tcactgggtt
     4381 ttccataaac ttctaattta tccattttag caacattgaa aatctttgga agtattgtct
     4441 gcgatacaat accgcttgtg gttgaatttt gatcctgtta tatattttct tttaggcatt
     4501 attttttgtt atattcatca tgacgacgaa cccacgccat aggaaagtca taaccatcct
     4561 cgtcccgtcc ctttggaact agatcaagat agttgtatgt attattcaat atatctattc
     4621 cacgtgcata agcagaatat gtgtgaaata ttttacctga tggatcttta taaaagacac
     4681 tcaccccagg cagttctgaa acatatggat tttgtatggt aaaattgtag aaaccttctt
     4741 tcttttctaa ttcttctgat gtaaatgata cataataatc acggttaaaa tctgtatcaa
     4801 aagaagaaac ccacttaaaa tcccaaccca tccgcttctt gtatgcatct atcttactat
     4861 aaggcgcttt agaaattgct atcatggtca catctctttg attcaaatgt acaaatatgt
     4921 cattaaaatt gtctgcccaa aaagaacatg acggacatgc tgcatcccac gtaggatcat
     4981 acatcaaatg atatactatt agctggcttc gtccatcaaa aagttctgac aatgtttgtt
     5041 ttccatttgg tccatcaaag acatattctt tattgacctg aacccaaggt agatctcgtc
     5101 gttgttgact aatttggtca cgtaatatag taaattcttt ttctttttta agtagttctt
     5161 tccgagcttc tatccattca ctttcagaaa caactttatg attatctaaa atgtacgtca
     5221 ttgtttttgc cacgcctcct tttctttatt ttcaagtttt ctacaatagt tagtcaactc
     5281 ttttaaaaat aaagtccaat ccctattgtg ttgttttata gatgttgaat acatacacat
     5341 tatatacaat atataatatt taaacattta aataattctt taattatcat attatgataa
     5401 tgactggttc tgtttggaaa gcactagctg aggataacag aagacagatt ttattattac
     5461 ttagggaaca tgacatgttt ccttcagagt tagcaaaaca ttttgatacc acttttgctg
     5521 cagtatccac aaatttaaga attttaaaag acgcagatct catcaaagaa actagagaag
     5581 gacagaaaaa aagatactct ataaatccta aaaaaactct agaaataaaa gaattttttg
     5641 ataaaatgtg gggacataac cttgaaggcc ttaaagagtt tgtagaaagt aaagaggaaa
     5701 agaaaaaaaa tgagcgataa atatgatgta cttaaaaaag agatagtaat aaatgcacca
     5761 gtagagaaag tatactctgc tctaactact ccagaacaat taactcaatg gtttcctaat
     5821 atagtaacta tagaaccaaa gataggtgga aaaatttctt ttagattctt aaaagaaagt
     5881 acacaagaag ataaagacca tgaaattgtt ggtgagattg ttagttttat accaaataaa
     5941 gagctatcct atacttggaa ttttacaaca aagcgtgaat ataataaaaa tacaattgtt
     6001 acgtggaagc ttgagcaact agataaggat aaaacaaaat taactttaat tcatagtgga
     6061 ttcacaaatg cagatagatt acaatatgat gatcataaca aaggatggag ttggcatatt
     6121 aaaagacttg aaaatcttat ggataaaaac acaaatgcaa acgaaattgt aatgcagtat
     6181 ttgcaagctg tggaaaacag ggattttgaa tctgctagaa aatatgtaag cgacaacata
     6241 tcctagtaga tgataatgat gtatgtctta tctctgacat gaatttcgac aacagtctgt
     6301 aacagcattg atattttcat ggtaccacgt taatgatgga aaaataagtt cgataagagt
     6361 agctttgatc cacggccttt cactcagaaa aattagacaa ataataaaaa agataaaaaa
     6421 tgaaataacc atagaatcag ctaatgtcat catgtcattc ttgctttcat taattttttc
     6481 tctgatgcac ttgtatatgg gtaaagtatg ataaaagttg taggttaaaa atattagtta
     6541 aagaatattc tataaatgat tacaattcaa acgtatcaat aacctcttct aatttgttaa
     6601 tagcctcatt cagcttattc tgatctacag ttggaatttc cattatctca tagttatcat
     6661 tttccgactt tgtaaaatca acataaaata ttcctaacct tgcttcagtt tgtgcaacta
     6721 atcctatggt aaaacttctc cacgaaaaag catcgggagt gaaagcacca tcatcattca
     6781 atataataca tctaccataa ccatcattct gtaaataatc aaatgcttta ttgaatttaa
     6841 agtcatgaga gcgaaatcca tctctatatt ttactatgta ttttccatca acagtttggg
     6901 attcttttaa taacaacagt tttccaaatt cttccaaagc ataaatgtaa attccagcag
     6961 caatctctat atcaatatct atattctgtt ttgcagcaga aagtttctta attataccgt
     7021 tttttattaa ccgcttagct ccattccatg tttcttttga tatgtggatt tcttgtgata
     7081 tgtgtggcaa atatattatt acatagaaaa atacaacttt aatcttttca ttggtttacc
     7141 tacacgtaaa ataacccact atttatgcac ttttaaacct gatacactag aagtatattg
     7201 cttttcctct tttgagttag tggtcccgag aaaactacgg gcccgtcggg agttgaatga
     7261 acacagttta cttgttcaat tgttaactaa cagataaact aattacatta acataaagat
     7321 tagacgcaat agaatgatca aatcgagatt acaaatggat atattaagaa aatttataaa
     7381 ataatattta tggctgcatc taatgagcta aaacttggaa tactcgtaag gattactgat
     7441 aacataaaaa aatacaaagg ttcttgacgc tgctgaaagg cagttctaga atatcatcct
     7501 cttaaaaata aacaataaat aaaatccatt aaaagtaaga gtagaggagg tgtcagaata
     7561 acaagaaatg acaataagag caattgaagt ttgggatgct gcagataggt ctgaaagtct
     7621 atatcaaaga atcaatgacg tattaagaga taataatatt actagagaag aacttattga
     7681 tataaaatat tctactgttt atatcccaga ggataaaaaa ataaccagtc aagcattaat
     7741 aatatttgat aagaaagaag aaattgtcat gacagaatta gatgtagttg atgataaaga
     7801 tgtacttaga gattaggaaa tgagttcttc ttcttcttct attactacta ctactaataa
     7861 taataataat aaaggtaaac cctgtgccaa tttggccgag ggtggagata ccctatgaaa
     7921 aattttcaca ggagaagact agagaaaact ctgctaacac tcccggtaaa ttaccggaag
     7981 tgatgaaact aggatatagt tgctaatttt gtttatatag gaatcaataa aataaaaagt
     8041 aacgaatcca gttcgtgtaa tatgttatta gaaatattta taatgtgggg atgtgtcgac
     8101 ttgaaagatt gtagtggtgc aggagattta tccaatattg cttctactta tataggtatt
     8161 ataataggag cattactcgg gggcttaatc agttggttga tatacaatag acaagagaaa
     8221 atttctaaga cacaagatac aatgctaagt ggtatcaaaa aattacaaga caaacaagaa
     8281 catattttag aaaagataca aacctttgag cagaatcatg acataatatt aaacgatatt
     8341 tttatgttgg agaagaaaat agattcatta ttacaaagaa aataatttaa tttagataga
     8401 aaatcaattt tattatttat attgaaaaat acttctttcg ataagaggga tcctcctaca
     8461 actttctacg ttttattttt tatcagaatc acttgcaaaa gtagcatcac ctaaatgatg
     8521 atgcatgtaa aaccttctcc taagaggttt tttatgctat tataaattta ggaaaagttt
     8581 tatttttttg gtatatctat ttctagtggt tgtaatttac tgggaggtac acaattaagg
     8641 caaaaatttt tcaagttgtc atattgaaca tgacattgtg gacattcttt tataatcatt
     8701 gtat
//
LOCUS       DASVEV010000308        16547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1140424, whole genome shotgun sequence.
ACCESSION   DASVEV010000308 DASVEV010000000
VERSION     DASVEV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16547
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140424"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF222_13380"
     CDS             <1..362
                     /locus_tag="VF222_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HEX7143462.1"
                     /translation="EKIRCQRRIRMVIMNMSKSNEDTTHVYTINLGKAWLTPQYRRTD
                     RVINMIKEFAKRHMKNENIKIDQELNKEIWKQGKTNPPRKVRVRMSLDDNVVLVSSYQ
                     ENLKENEVKKNENNDST"
     gene            complement(464..772)
                     /locus_tag="VF222_13385"
     CDS             complement(464..772)
                     /locus_tag="VF222_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143463.1"
                     /translation="MTHNEIDIDQFILLTIYPSIALFGITFFAKKAKLGSSIQYCLQA
                     FTSIVFSVTYLIFIPFGGAQGLALVLLLFSGILLLMARKHRIHPEENDKSSSREIPFI
                     "
     gene            complement(823..1404)
                     /locus_tag="VF222_13390"
     CDS             complement(823..1404)
                     /locus_tag="VF222_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143464.1"
                     /translation="MSDNMEDYFTLEMAYNILLKEYQISSLQEIPYDTYQRLSITIGI
                     LKTKEYGIIEKKIVQKIIENLSIISNLLLQTRIEKILIGNINSNLEMGYSKMTDEEKF
                     IIDGEIQSKRKRKEIMTLITNGQSKILEKITNTIKQKKLLIMFRKPMEQFVGVDMYKY
                     GPFSVDDVANLPFENALSLVNNKIATEIAPIFD"
     gene            1505..2971
                     /gene="glnA"
                     /locus_tag="VF222_13395"
     CDS             1505..2971
                     /gene="glnA"
                     /locus_tag="VF222_13395"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7143465.1"
                     /translation="MPYRIIEGRIEQQNFGTDEIMDKLEKDDVKFIDLQFTGLFGKFH
                     HITISTNMFKKTDFENGLPKVDGSSIHGFTEIHESDLIVRPDPSTYAIIPWIDKYKTC
                     RLICDIIRGGSNRSQFLRDPRGIAKKAEKHVNENGYALSYWGPEVEFFVFDKLLVDTL
                     TPYRGQSYEIISKEAPWASDGFGYTLNLKEGYLPSAPADSLMDFRNECVDILSNNFGI
                     ACDAHHHEVATAGQCEIDIRYDTLVNAADSVQSYKYIVKNVAKKHNMIATMMPKPIAL
                     DNGSGLHVNISLWNNNSNLFFDQNDEYAEISELARYFCGGILEHSFALAAIVAPTTNS
                     YRRLVPGYEAPVYVAWSTGNRSAIIRIPGHFRGEKFANMKRIEFRAPDPSCNPYLAFS
                     CIVAAGLDGIKKKTSIGDPIDEDIFKMTSKRRRQLGIKQLPTSLLDASEALDSDREFL
                     KPLFDGSVIDRLIEHSTNEYKEVTLRPHPHEFTLYADV"
     gene            complement(3022..3285)
                     /locus_tag="VF222_13400"
     CDS             complement(3022..3285)
                     /locus_tag="VF222_13400"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7143466.1"
                     /translation="MANEVEVKLKELRNWKIENGKLCKSFEFKNFTQAFGFVTKIALE
                     SEKLDHHPELFNVFNKVNISLSTHKINGLSEYDFILAKKIDDL"
     gene            3456..4763
                     /gene="tuf"
                     /locus_tag="VF222_13405"
     CDS             3456..4763
                     /gene="tuf"
                     /locus_tag="VF222_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HEX7143467.1"
                     /translation="MSSSKKPHMNLVVTGHVDNGKSTIVGHLMVDLGVIDQRTIEQFA
                     KESEATGKGDTFKYAWVLDSIKDERERGITIDLAFQKFETNKFFFTLIDAPGHRDFIK
                     NMITGASEADVAILVVSTKPGETEAATEPGGQAREHAFLCRTLGVGQVVVALNKMDDS
                     NYSEARYNEVKGIAEKMLKMVGYNTTKVNFVPVSGWKGDNLAKKSENMGWYKGPTLLE
                     ALDSFDPPEKPIGKPLRIPIQDVYSITGVGTVPVGRVETGVVKANDKVIVMPAGVTGE
                     VKSIETHHTQMNSAEAGDNIGFNLRGIDKKSIHRGDVIGHPDNPPSVAKEFEAQIIVI
                     HHPTAMAPGYTPVLHAHTAQVAATLSDFVAKIDPKTGGVLEEKPKFLKTGDAAIVRIR
                     PVRPLAIETFKEFPEIGRFALRDMGTTIAAGVIKSITEKHEKK"
     gene            4779..5087
                     /gene="rpsJ"
                     /locus_tag="VF222_13410"
     CDS             4779..5087
                     /gene="rpsJ"
                     /locus_tag="VF222_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7143468.1"
                     /translation="MPQAARIKLTSTNLSTLEGVCSEIKGIGEKSGINLKGPYPLPTK
                     KMKVVTRKSPCGQGTNTWDKYELRIHRRVIDLGADDKAIRQLMRLRIPDDVYIEVSLT
                     "
     gene            5178..5741
                     /locus_tag="VF222_13415"
     CDS             5178..5741
                     /locus_tag="VF222_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143469.1"
                     /translation="MNLFWSVVLLQSLLLLLFNFNQIFAESPTTDFKPYQNKKHSVEL
                     MYPSDWTYVEFRDQFSDNDLSIITSFISPLDSSVDTFQEYFTIKSKILDPGDTFSNHY
                     KSYLEKLKETITNINISNIENTSNINKYIQYSFSPQPGLVINKDEYIFLINNNNNVFQ
                     IEFTSNGDDYKAFKLLINKIISYFRII"
     gene            complement(5786..6409)
                     /locus_tag="VF222_13420"
     CDS             complement(5786..6409)
                     /locus_tag="VF222_13420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7143470.1"
                     /translation="MSKYELPPVPYNYDALEPYIDARTMEIHHTKHHQTYTDKLNTAL
                     EKCPTEIQEKDIVEILSNLDQVPADQKSAINFNGGGFDNHRIFWHNMKPNGGGEPTGS
                     IADAIKSSFDSFSGFKEKFSSTTALIQGSGWGWLVYNPSTNKVEYKSMPNQTSPRTEG
                     LIPLLGCDVWEHAYYLKYQNKRPDYISAWWNVINWEDVSQRFDKINK"
     gene            6546..6968
                     /locus_tag="VF222_13425"
     CDS             6546..6968
                     /locus_tag="VF222_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HEX7143471.1"
                     /translation="MVINKELLEKRKKVSEHRPKFVRQESWRYDRLAENWRRPKGKDN
                     KMRLQVSGVPQLVKVGYRGPKAARGLHPSGYTDNLVYNINDLLILDSKKDAVRIGHIV
                     GKLKRKQILTKADELGFKVLNPGKDSKLRNKSQIKENE"
     gene            6972..7418
                     /locus_tag="VF222_13430"
     CDS             6972..7418
                     /locus_tag="VF222_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HEX7143472.1"
                     /translation="MVVNLKKKRELVARMLNVGSNRVKFEPDKLDDIADSITREDIRS
                     LIKNGVIWTSKPKGTSRGKAKAKLETKKKHGTGAGSKKGRKTARTGKKELYVKKIRAM
                     RYHLKVLKDRNDVNRETYWSLYKKINGGQIRSLTHLRSTVKDWKSQ"
     gene            complement(7442..7639)
                     /locus_tag="VF222_13435"
     CDS             complement(7442..7639)
                     /locus_tag="VF222_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143473.1"
                     /translation="MSNWEENNQDDRDDLHILTLPNIKYLVDGLNVLLQTNLDGEKRS
                     QVIRLREKLINISSSIINDFN"
     gene            7741..8547
                     /locus_tag="VF222_13440"
     CDS             7741..8547
                     /locus_tag="VF222_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX7143474.1"
                     /translation="MFFTLITSEMDPASKTMIEYLQENKKFKEIKKFYFKSSLYDNIS
                     MFISKNPLIYADSIDALCNKTDALIFLSKHQSKSKIPSLTCHFPGNYYTNSYGGNKFE
                     LGIAYPSLQKNYLVKIFQIREKVPLCDITIETTHHGPTSLKKPVMFVEIGSSEDQWKN
                     IENASFVCDSLLDTLTKKITHAKDIGIGLGGNHYGSKFNKLILNTEYGIGHIANKYNL
                     VNINKDMLNQMISKCYEKVTHIIIEQKSLGNEKQRIMNLIKDIELEVIKI"
     gene            9015..9425
                     /locus_tag="VF222_13445"
     CDS             9015..9425
                     /locus_tag="VF222_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143475.1"
                     /translation="MKKFSEKPPMIVISGSCLNFDKERKKILEFIEYLQFSVRSEINC
                     EVSSNGAALGWDFFNIYFDLSLMENLIVYFPEISKEEGINHEQQFVLWLTKQFKRRNL
                     DYHLKLSDIPYENTKGFRLDPNNYRDDSLLEDLR"
     gene            9584..11386
                     /locus_tag="VF222_13450"
     CDS             9584..11386
                     /locus_tag="VF222_13450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7143476.1"
                     /translation="MNLLQKPQLGHWDLSELVDEKENIIKNYLKILHVDVKKFENNRN
                     LLNDNISSQKFSEMLLQLEKISEKLNMVIGFAHLQYSSNTSSNEYSALVTEMELLGTE
                     ISNNLIFFDIWFKNGLKEKDANRIIQTVDPVYKHFLTHKRILSKYTLSEKEEKIVNIL
                     EVSGPTALVKIYDRMTNSFEFVLEIKRNNKITRKKFINKEKLMSLVRSNKSEERKAAY
                     KSLFNVYKNNSGVLGEIYLNLIVQWHDENIKIRGFDSPISVRNVYNDISDSVVSTLLD
                     TCKKNSFIFHKYFNLKAKMLKLKKLERYHLYAPFVKKGMVQKRYTYEQAVNIVLNVFE
                     SFDPKFREYAEIVFNKNHIDAEIRKGKMSGAFCSTITPKLTPYILLNYDGMYRDISTM
                     AHEFGHAIHSICSSNLPIAVSHAPLPLAETASVFAEMLLNEKLAEEMTEQERSILLAE
                     QIDDMYATIMRQSYFTLFEIDAHSKVIENNANIDNVSNLYKKNLQEQFGNSVIVSDDF
                     MWEWTYIPHFYHTPFYCYAYAFGNLLVLSLFNMYKKEGKSFIPKYLNLLSAGGSKKPE
                     TLLTEIGFDISNKKFWQQGFDLLSEKIGDLENIV"
     gene            complement(11800..11952)
                     /locus_tag="VF222_13455"
     CDS             complement(11800..11952)
                     /locus_tag="VF222_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143477.1"
                     /translation="MHPEDLNKIWFCEDCQSSFFFNSDAEDHKEKTGHVKMRKFSSVS
                     GGIIPK"
     gene            complement(12142..12291)
                     /locus_tag="VF222_13460"
     CDS             complement(12142..12291)
                     /locus_tag="VF222_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143478.1"
                     /translation="MESAIVALENCNLETGINQEEFECLRSYLGFDNEEELKSYLKHY
                     FVSKV"
     gene            complement(12383..13768)
                     /locus_tag="VF222_13465"
     CDS             complement(12383..13768)
                     /locus_tag="VF222_13465"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX7143479.1"
                     /translation="MSFRIDKDSLGEFKVPDTAYYGPFTARAIEQYKVTGNKSHINLI
                     KAYVMIKKSAALTNFELQVLDERIAKVIIQACDEILSGQFLDQFVVDAINSGAGTAFN
                     MNANEIIANRALEVLGKSKGDYMTVSPNDHVNMSQSSNDTFPTAMNIAILININLIIK
                     SLDVMIASLKMKSIDFKNIIKIGRTHLMDAIPVTLGSEFEVYAYALERAKEEIINSQR
                     KLEFIGIGGTAVGTGVNTPKGYREKIVKHLFNISGLNLKISENLHFFLQSKFDISNFS
                     SGLRNLALELIKIANDLRLMSSGPIAGLAEILIPAVHAGSSIMPGKVNPSLAECLNMI
                     CFDIIGKDVSVAMAAQAGQFELNVMMPGMLKNVLDATDMLINFLPIFATNFINGIQAN
                     SERLKQQVENSPILITLLNPYLGYMKAAEIYKESLRTGRGIRELILRNNLMSKKELED
                     ALSYEKIIGLV"
     gene            13905..14468
                     /locus_tag="VF222_13470"
     CDS             13905..14468
                     /locus_tag="VF222_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX7143480.1"
                     /translation="MPQTKPIVSIENVVASATINQTVNLNQITQIFPDVEYHPDQFPG
                     LVFRLKSPKTATLIFSSGKMVCTGAKSEDQAIKAVKSVVQKLKKGSIQIANEPIIEIQ
                     NIVASASLGGKIHLELAARVLPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGA
                     KKEAEVYRAVHNLHNLLEEKNLMIYEG"
     gene            complement(14469..15065)
                     /gene="pyrE"
                     /locus_tag="VF222_13475"
     CDS             complement(14469..15065)
                     /gene="pyrE"
                     /locus_tag="VF222_13475"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7143481.1"
                     /translation="MNDSYTTNLAEFLIQSNSLKFGNFKLASGKNSSFYIDLRLIQSF
                     PGIFHQTIIALRDIIQSKNLRFDMLATIPTSGLIFTSALAYEMFRPLIYLRKEKKGYG
                     INNLVEGRLENTQKILLIDDVVTTGQSLNIAIENLRNNGGIVENVLCILLRGDNTTIK
                     NFNAIGINLHFLLTINELANILYKNQWINDEKKLSLQK"
     gene            15115..16221
                     /locus_tag="VF222_13480"
     CDS             15115..16221
                     /locus_tag="VF222_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143482.1"
                     /translation="MPEIWLGYGSTDVVLDIKQENLLSCYPKLDLLSNENIKEILTNI
                     KFSKKTLIFPLSSSKSILKIISFLIEINKTDITKEIEIGTFSDSFLQIKNHYDDNIRI
                     FQMDRNDFYEKIKKFDNVIFISKMGYEPLFGFSGTPSILIRSFLNGLMNEIFHSNEII
                     RPNSGQIREPLKIALEFYDKLGYYSIEVLSNQYGISRLYYGFNSSFIKSTISDFQSNT
                     NFNLNEQKSLILSAGPEPENHITLTNSLYSLWNSIGVLKNQGTAVLLAENSHGIGEGA
                     LTMYLEGRLNLSEIKKINYVNGLEHLVYLENLKNEYHLGIVSSIPFHYTKTKLGFNTF
                     NNVKEVLQNMVLRYGKSSKVLICPDPNITVFIKQ"
     gene            16328..>16547
                     /locus_tag="VF222_13485"
     CDS             16328..>16547
                     /locus_tag="VF222_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine acetyltransferase"
                     /protein_id="HEX7143483.1"
                     /translation="MQSILRSVGGYVIRNCEEKDLPFVININMITLPEHYSDYFFTSI
                     LAELPEAFIVAEIKNQIVGYIMCKIEFGF"
BASE COUNT         5994 a         2324 c         2506 g         5723 t
ORIGIN      
        1 tggagaagat ccgatgtcaa cgtaggataa gaatggtgat tatgaatatg tcaaaatcta
       61 atgaggatac aactcatgta tataccataa atctaggaaa ggcttggtta actcctcaat
      121 atcgacgaac tgatagagta attaatatga ttaaggaatt tgccaaacgt catatgaaaa
      181 atgaaaatat aaaaatagat caagagttaa ataaagaaat ctggaaacaa ggcaaaacaa
      241 atccaccacg aaaagtacgt gtacgaatgt cccttgatga taacgttgtt ttagtatcat
      301 catatcaaga aaatttaaag gaaaacgaag taaagaaaaa cgaaaataac gacagtacgt
      361 aaacagatac taataaattc tactcttaat tctgattttt aaatattatt cctctattct
      421 gatttaaatt tttatcatga taagtttctt gtatactata catttaaata aatgggattt
      481 ctctagatga tgatttatca ttttcctcag ggtgaattct atgttttcta gccattaata
      541 ataatatacc actaaacaat agtaacacta gtgctagccc ttgagctcct ccaaatggaa
      601 taaaaatcag ataggtaact gaaaatacaa tacttgtaaa agcctgtaaa cagtattgga
      661 tagatgatcc taacttagcc ttttttgcaa aaaatgtaat tccaaatagt gcaatacttg
      721 gataaattgt tagcaaaata aactggtcaa tatctatttc attatgtgtc aataacttag
      781 tgaatattat tcataatatt tttctttctt ttcaattaaa tattaatcaa atataggggc
      841 aatctcagtt gcaattttat tattaacaag agataatgca ttttcaaaag gtaagtttgc
      901 gacatcatca acgctaaaag ggccatactt atacatatca actccgacaa attgttccat
      961 tggttttcta aacataatta gcaatttttt ttgtttaatt gtatttgtta ttttttctaa
     1021 aatctttgac tgaccattgg ttattaaggt cattatttct tttctcttcc tttttgattg
     1081 gatttctcca tcgattataa atttttcttc atctgtcatt ttagaatatc ccatctctaa
     1141 attagagttt atatttccta taaggatttt ttctattcta gtttgcagta aaagattaga
     1201 tattatcgat aaattttcaa taattttctg tacaattttt ttttcaataa taccatactc
     1261 ttttgtcttt aaaattccaa ttgtaataga taatctctga tatgtatcat aaggtatttc
     1321 ttgtaatgaa gaaatttgat attcttttag aagaatatta tatgccattt ctagtgtgaa
     1381 gtaatcttcc atattatcag acattaattt aatattgtat gtgaatcaag ttataaataa
     1441 ttccgttttt ctatttaatt tctgaaagcc ttaattatga gatccttaat tattaccaac
     1501 tgatttgcca tataggatta ttgaaggaag aattgaacaa caaaattttg gcactgatga
     1561 gattatggat aaattagaaa aagatgatgt caagtttata gatttacaat ttactggact
     1621 ttttggaaaa ttccatcata ttactatttc tacaaatatg tttaaaaaaa cggattttga
     1681 aaatggatta ccaaaagttg atggttcttc aatccatgga tttactgaaa tccacgaatc
     1741 tgatttaatt gttagaccag atccttctac ttatgctata attccttgga tagataaata
     1801 caaaacgtgc agattgattt gcgatattat aagaggagga tctaatagaa gtcaatttct
     1861 gcgggatcct cgaggtatag caaagaaagc tgaaaaacat gttaatgaga atggttatgc
     1921 attatcatat tgggggccag aagttgaatt ttttgttttt gataaattgt tagtagatac
     1981 tcttaccccc tatcgtggtc aaagttatga aataatttca aaagaagctc cttgggcttc
     2041 tgatggcttt ggatatacat tgaatctaaa ggaaggatat cttcctagtg ctccagcgga
     2101 tagtttaatg gatttccgca atgaatgtgt agacattcta agcaataatt ttggtattgc
     2161 ttgtgatgca catcatcatg aagttgcaac agcgggacag tgtgaaatag atatacgata
     2221 tgatacatta gtaaatgctg cagattcggt ccaaagttat aaatatattg taaaaaatgt
     2281 tgccaaaaaa cacaacatga ttgctactat gatgccaaaa ccaatagcac ttgacaatgg
     2341 atctggttta catgttaata tcagtctttg gaataataac tctaacttat tttttgacca
     2401 aaatgatgaa tatgctgaaa tttctgaatt agctagatat ttttgtggag gtatattaga
     2461 gcattctttt gcacttgccg caattgtagc cccaacaact aactcctata ggcgattggt
     2521 tccaggatac gaagctcctg tgtatgttgc ttggagtact ggtaatagat cggctataat
     2581 acgtattcca ggacatttta gaggagaaaa atttgcaaat atgaaaagaa ttgaatttcg
     2641 tgctcctgac ccctcgtgta atccatatct agctttttct tgtattgttg cagctggctt
     2701 agatggaatt aagaagaaaa catctatagg tgatcctata gatgaagata ttttcaaaat
     2761 gacctccaaa cgaagacgtc aacttggaat aaaacaatta ccaacgagtt tattagacgc
     2821 atctgaggct ttagattccg atagagagtt tcttaaaccc ctatttgatg gttcagtaat
     2881 cgatagattg atagagcatt caacaaatga atataaagaa gttaccttac gacctcatcc
     2941 acatgaattt actttatatg cagatgtata aagcacttga gttattgtta attaatattt
     3001 aatttataga agtttacagt cttaaaggtc atcaattttc tttgccaata taaagtcata
     3061 ttcacttaac ccattaattt tgtgtgtaga taaagaaata ttcactttgt taaatacatt
     3121 aaataattct ggatggtgat ctaatttctc tgattctaat gctatttttg taacaaaacc
     3181 aaaggcttgg gtaaaatttt taaattcaaa agatttacat aatttaccat tctcaatttt
     3241 ccaattcctt aattctttta acttaacttc aacttcatta gccaaaagtt ttttgtaatt
     3301 attcaactaa aaaatatata atcgattact attaatgtat tttactgtag ttctttatta
     3361 gagtaaaaga catttttttt atggtcacct tttcatatta tattcaattt ttgataatcg
     3421 aaatataaat aaagcagata aatactcttc cgtgtatgag ctcaagtaag aaaccacata
     3481 tgaatctcgt ggttacagga catgtggata atggtaaatc tacaattgtt ggccatttaa
     3541 tggttgattt aggagttatt gatcaaagga ctatagaaca atttgcaaaa gaatcagaag
     3601 ctactggtaa gggtgatact ttcaaatacg catgggtttt agatagcatt aaagatgaaa
     3661 gagaacgagg aattaccata gatttagctt ttcagaaatt tgaaactaac aaattcttct
     3721 ttactttaat tgatgcccct ggtcataggg actttataaa aaatatgata actggtgcct
     3781 cagaagctga tgtggctatt ttggttgttt ccacgaaacc aggagaaaca gaagcggcaa
     3841 cggaacctgg aggtcaagca agagaacatg catttctatg cagaacattg ggagtcggac
     3901 aagtagtagt tgcattaaat aaaatggatg attccaacta ttcagaagca agatataatg
     3961 aagtcaaagg tattgcggaa aaaatgctga aaatggtggg ttataatacc actaaagtaa
     4021 attttgtccc agtatccgga tggaaaggtg ataatttagc aaaaaaatct gaaaatatgg
     4081 gctggtataa aggtccaact ttgttagaag cactagattc tttcgatccg ccagaaaaac
     4141 caattggtaa acccttgaga attccaattc aagatgtata ttcaattacg ggtgtaggta
     4201 ctgttccggt aggcagagta gaaacaggtg ttgttaaggc taatgataag gtaatagtca
     4261 tgcctgcagg agtaactgga gaagtcaaat ctatagaaac gcatcatacc cagatgaatt
     4321 cagctgaagc tggtgataat ataggattta atttaagagg aattgacaaa aaatctattc
     4381 atagaggaga tgttataggg catccagata accctccaag tgttgctaaa gaatttgaag
     4441 ctcaaataat agtaatccac catccaactg ctatggcacc tggttacacc ccagtattac
     4501 atgctcatac agcacaagtc gcggcgactt tatcggattt tgttgccaaa atcgatccta
     4561 aaactggcgg agtcttggaa gaaaaaccaa aatttcttaa aacaggagat gcagcaattg
     4621 tgagaattag accagttaga cctcttgcta tagaaacttt caaagaattc cctgaaatcg
     4681 gtagatttgc attaagggat atggggacta caattgctgc tggagttatt aaatccatta
     4741 cagaaaaaca cgaaaagaag taaaagtgaa atcttacaat gccccaagca gctagaataa
     4801 aactaacaag caccaatctt tctacattag aaggtgtttg ttctgaaata aaaggaatag
     4861 gtgaaaaaag tggtataaat ttaaaaggtc cttatccact gcctacaaaa aaaatgaaag
     4921 tagtaacaag gaaatctccc tgtggacaag ggacaaatac atgggataaa tacgaattaa
     4981 ggattcatag acgagtaatt gatttaggtg cggatgataa agctattaga caacttatga
     5041 gattgagaat acctgatgat gtatatatag aagtatctct tacctaaatt tttctctttt
     5101 atgtctaaca aataatcttt gtaccaattg ttaccatatc taagaaataa aatttactaa
     5161 ttagtaaaga atagaaaatg aatttatttt ggtcagtcgt gttattacaa tcacttttat
     5221 tattactctt taattttaat caaatttttg cggaatcacc tacaactgat tttaaaccat
     5281 atcaaaataa aaaacattct gtagaattaa tgtatccctc agattggaca tatgttgaat
     5341 ttagagatca attttctgat aatgatctat ctataataac tagtttcatt tctccattag
     5401 attcttctgt agatacattt caagaatatt ttactattaa atcaaaaatt ctagatccag
     5461 gagatacgtt ttctaaccac tataaatcat atttagaaaa actcaaggaa actataacaa
     5521 atataaatat atcaaatata gaaaatacct cgaatataaa taagtatata caatattcat
     5581 tttctccaca accagggcta gttattaata aagatgaata tatctttcta ataaataata
     5641 ataataatgt atttcaaatt gaatttactt ctaatggtga tgattacaaa gcatttaagt
     5701 tattgataaa taaaataata tcatattttc gtataattta acatgacatt tgatgtagta
     5761 taaaaaatta ttgaggagaa aacctctatt tgttgatctt atcaaaacgt tgtgaaacgt
     5821 cttcccagtt tataacattc caccaagcag aaatgtagtc agggcgtttg ttctgatatt
     5881 ttagatagta tgcatgttcc catacgtcac atcctaaaag tggtatcaaa ccttctgttc
     5941 ttggacttgt ctgatttggc attgatttgt attctacttt attagtggat ggattgtata
     6001 caagccatcc ccaaccacta ccttgaatca atgcagtagt agaagaaaat ttttctttaa
     6061 agcctgaaaa gctatcaaat gacgacttta ttgcatcagc aatagacccg gttggttcac
     6121 cccctccgtt tggtttcatg ttgtgccaga atattcgatg gttatcaaat cctcccccat
     6181 taaagttgat tgcacttttt tgatcagctg gaacttgatc aagattggat agtatttcca
     6241 cgatatcttt ttcttgaatt tcagtaggac atttctcaag tgctgtattg agtttatctg
     6301 tataagtttg atggtgtttt gtatggtgta tttccatagt tcttgcgtca atatatggtt
     6361 ctagtgcatc ataattatat ggtacaggtg gtaattcata tttgctcata catgtatttt
     6421 tttttgtaat tattaacctt tgtgttatct agtatttttt tgatgtctcc ttaattttaa
     6481 tctattactt tgttaaaggg aaacatattt attgaaattt aaaattctat tttaactgct
     6541 tactcatggt tattaataag gaactgctag aaaaacggaa aaaagtatcg gaacaccgac
     6601 ctaaatttgt aagacaagaa agctggagat acgacaggct tgcagaaaac tggaggaggc
     6661 ctaaagggaa agataataaa atgagactac aagtttctgg agtccctcaa ttagtaaaag
     6721 ttggatatag aggcccaaag gccgctagag gacttcatcc atcaggatat actgataatt
     6781 tagtatacaa tatcaatgat ctgttaatat tagattcaaa aaaagatgct gtaagaatag
     6841 gtcatattgt tggaaaatta aagagaaaac aaattttgac taaagcagat gaattgggat
     6901 ttaaagtttt gaacccagga aaagattcta aattacgaaa taaatctcaa ataaaggaga
     6961 atgaatagat attggttgta aatcttaaaa agaaacggga attagtcgct agaatgctta
     7021 atgtgggttc taatagagtt aaatttgaac ctgataagct tgatgatata gcggattcta
     7081 taacaagaga agatataaga tctttaatta aaaatggtgt tatatggaca tcaaagccca
     7141 aaggaacttc tcgaggtaaa gctaaagcaa aattggagac aaaaaagaag catggaaccg
     7201 gcgctggatc taagaaaggt cgcaaaacag caagaacagg aaaaaaagag ttatacgtga
     7261 aaaaaataag agcaatgaga taccatttga aagtattaaa agatagaaat gatgttaacc
     7321 gtgaaacata ttggtctttg tataaaaaaa ttaatggtgg tcaaattaga tccttgactc
     7381 acttaagaag cactgtaaaa gattggaaat cacaataagg gttcaaaaga taatatttgg
     7441 ttcaattaaa atcgtttatt attgaactgg atatattgat taatttttct cttaacctaa
     7501 taacctgaga cctcttttct ccatccaggt tagtttgaag caatacattc aatccatcca
     7561 ctagatattt aatattgggt aaagtaagaa tatgtagatc atcacgatcg tcttgattat
     7621 tttcttccca attactcaca ctctagaata ttttgcgtta atgttaaatg ttttggattt
     7681 caatttaata aattaattaa ggtaattatt aaaatattat acatttttac actagatcat
     7741 atgttcttta cattaattac ttctgaaatg gatccagcta gtaaaacaat gatagaatat
     7801 ttacaagaaa acaaaaaatt caaagaaata aaaaagttct attttaaatc ttctctgtac
     7861 gataatattt ccatgtttat atctaaaaat ccattaatat atgctgatag tatagatgcc
     7921 ctatgtaaca aaaccgatgc tttgattttt ctttctaaac atcaatcaaa aagcaagatt
     7981 ccatctttga catgtcattt tcccggaaat tattacacga attcttatgg tggaaataaa
     8041 tttgagttag gaattgcata tccatcattg caaaaaaatt atcttgtaaa aatattccaa
     8101 ataagagaaa aggttccttt gtgtgatata actatagaaa caactcatca cggtcccact
     8161 agtttaaaaa aacctgttat gtttgttgaa ataggttcct ctgaagacca atggaaaaat
     8221 attgagaatg catcattcgt atgtgattct ttattagata cattaacaaa aaagattact
     8281 cacgcaaaag atattggaat aggattagga ggaaatcatt atggttccaa attcaacaaa
     8341 ttaatactaa atactgaata tggtataggt catatagcaa ataaatacaa tttagttaat
     8401 attaataaag atatgctaaa tcaaatgatt tcaaaatgtt atgaaaaagt tacacatata
     8461 ataattgaac aaaagagttt gggcaatgaa aaacaaagaa taatgaatct aattaaggat
     8521 attgaattag aagtaattaa gatataaata attaaatata agttccctat aattacaaat
     8581 taaaatactt tttacgtatt gttttaaatt ttataaggaa aacaatgtta aaagaaggaa
     8641 agaatataag tcaaaataag aaaaaagaga tatactaagt gttctccagg aaaggagcat
     8701 ccataactgc tttatcgtga atttatgaga agacagaaat cattagaact attcttatag
     8761 actataattt atggattttt atcttatttc atttcaatag ttcttttgaa tatttattct
     8821 cgtttttctt ttttgacatt tacgaaactt taaaacttaa ggaaatttta cctctactta
     8881 aatttgccag tcgtaattat taaaccggag aatgccaaaa cttaaggata tcttattaag
     8941 ttcattttaa caaccaatca aataataaaa gttttttagc tttgttttta tattcttatt
     9001 ctatatatac agtattgaaa aaattctcag aaaaaccacc aatgattgta atatctgggt
     9061 cttgtttgaa ttttgataaa gaaagaaaaa aaattcttga gtttatagaa tatttgcaat
     9121 tctctgttag atcagaaatt aattgtgaag tctcatctaa tggggctgct ttaggatggg
     9181 atttcttcaa catatatttt gacctttctc ttatggaaaa cttgatagta tattttcctg
     9241 aaatttccaa ggaagaagga ataaatcatg aacagcaatt tgttttatgg cttacaaagc
     9301 aatttaaaag acgaaatttg gactatcatc ttaagttaag tgacatccct tatgaaaata
     9361 caaaaggatt cagattagat ccaaataatt atcgggacga tagtttgttg gaagacttaa
     9421 gataaattgt cctatcaaac aatatttgat tcatatatta ataatttcta tattactggt
     9481 ataatatgat tgtaattaga tttagaatca atattcaaca gacgttataa gaattaacac
     9541 ttaattgata actattaata caagactata aattttttat attatgaatc tattacaaaa
     9601 acctcagtta ggacattggg atttatccga attggtagat gagaaagaaa atatcataaa
     9661 aaattattta aaaattttgc atgttgatgt caaaaaattc gaaaataacc gtaacttatt
     9721 gaatgataat atttcttcac aaaaattttc tgaaatgtta ttacaattgg agaagatttc
     9781 agaaaaatta aatatggtaa taggtttcgc tcatttgcaa tattcatcca atacttcttc
     9841 taatgaatat tctgccttag ttacagaaat ggagctatta ggtactgaaa tttctaataa
     9901 tttaatcttt tttgatatat ggttcaaaaa tggtctaaaa gaaaaagatg caaatagaat
     9961 aattcaaaca gtcgatccag tttataaaca ctttcttact cataaaagga ttttaagtaa
    10021 atatacctta tctgaaaaag aagaaaaaat tgttaatata ctcgaagtaa gtggaccaac
    10081 tgcattagtc aaaatatatg atcgaatgac caatagtttt gaatttgttc ttgaaataaa
    10141 gagaaataac aagataacaa gaaagaaatt tataaataag gaaaaactaa tgtcactagt
    10201 tagaagtaac aaatcagagg aaaggaaagc tgcatataaa tcattgttta atgtatataa
    10261 aaacaatagt ggagtgttag gtgagatata tttaaattta atagtacaat ggcatgatga
    10321 aaacattaaa atccgcggtt ttgattcacc catttctgta agaaacgttt ataatgatat
    10381 aagcgatagt gtagtatcta cattattgga cacatgtaag aaaaattctt ttatttttca
    10441 taaatatttc aatttaaaag caaaaatgtt aaaattaaaa aaactagaac gttatcactt
    10501 gtacgcacct tttgttaaaa aaggaatggt acaaaagaga tatacttatg agcaagcagt
    10561 aaacatagtt ttaaatgtat ttgagtcatt tgatcctaaa tttcgagagt acgcagaaat
    10621 agtttttaat aaaaatcata ttgatgcaga aataagaaag ggaaagatga gtggagcatt
    10681 ttgttctact attactccta aactaactcc atacattcta ttgaattatg atggtatgta
    10741 tcgtgatatc tctacaatgg ctcatgaatt tggacatgct atacatagta tttgttcttc
    10801 aaacctccca atcgctgtct ctcatgctcc tcttccttta gctgaaacag cttctgtgtt
    10861 tgctgagatg ttattgaatg aaaaattagc ggaagaaatg acagaacaag aaagatctat
    10921 cctgttagca gaacaaatag atgatatgta tgcaactatt atgagacaat catattttac
    10981 attatttgaa attgatgcac atagtaaagt catcgaaaac aatgctaata ttgataatgt
    11041 ttcgaattta tataaaaaga atcttcagga gcagtttgga aattcagtaa ttgtttccga
    11101 tgattttatg tgggaatgga cttacatacc ccatttttat catactcctt tctattgtta
    11161 tgcttatgct tttggcaatc ttctagtttt atcgttattt aatatgtaca aaaaagaagg
    11221 aaaatctttt atcccgaaat atttgaattt actttctgca ggaggttcaa aaaagccaga
    11281 aactttatta actgaaattg gttttgatat ctctaacaaa aaattttggc aacaaggttt
    11341 tgatttattg tcagaaaaaa taggtgattt agaaaatatc gtttagtttt atattaatat
    11401 ttacttagat atagttagta tactacattt taattgtaag attaatgaat tatttatttt
    11461 gttaatctag ttatattaaa ttacttcatt tgtttctata ctattgataa aagttccttg
    11521 ttatatggta atgataactc taattcataa tcctatcaat actaataaaa tattgcaata
    11581 ttctgataat gtatctaaaa gttttaacta aatattgtta tgaagttatt tttgaaaagt
    11641 ttctaattcc tgtaccccta tataatttca tgttaacaac aaatacaaat tttaaatctg
    11701 caactcgaac aattgataat tctcagaagc aaattcttca atatacaaat taaacgaatt
    11761 taacgatata taagcacagc aaataagcaa aaccgaattt tattttggta taattccacc
    11821 tgatacagaa ctaaattttc tcatttttac atgtccagtt ttttctttgt gatcttcagc
    11881 atctgaattg aaaaaaaaac tactttgaca atcttcacaa aaccaaattt tattcaagtc
    11941 ctctggatgc atagttaata aacaaatttc tgcaatataa gaaattagat aatatctaat
    12001 atataattta tagaaattaa gtgctacttc aaacttatat tttttttata aagtataatc
    12061 tttaggaaaa atcgtatatg atacttttat aatactgaat aaattgaatg aaagttctat
    12121 aaagttaaat atatttaatg gttaaacctt ggagacaaaa taatgtttta aataggattt
    12181 tagctcttct tcattgtcaa agccgagata gctccttaaa cattcaaact cctcctggtt
    12241 tattcctgtt tctaaattgc aattttcaag tgctactatt gcactttcca agctattttt
    12301 cctaatatta ttagaaaata agaatactta tacattctac acgtatgcaa aaagatttaa
    12361 taatattcta tccaaatcaa aattaaacta atcctatgat tttctcataa gatagcgcat
    12421 cttctaattc ctttttactc atcaaattat ttcgtaaaat cagttctctt atccctctac
    12481 ccgttcttaa tgattcttta tatatttcgg ctgctttcat gtatcccaaa tatggattta
    12541 ataaggttat gagaattgga ctgttttcca cttgttgttt taatctttca gaatttgctt
    12601 gaattccatt aataaaattg gttgcgaaaa ttggcaggaa atttattaac atgtctgtgg
    12661 catctaatac attctttagc attcctggca tcattacatt taattcaaac tgtccagctt
    12721 gtgcagccat ggctacagaa acatcctttc ctataatatc aaagcaaatc atgtttaaac
    12781 attcagccag agaaggattg acctttcctg gcataataga agaacctgcg tgaactgctg
    12841 gaataagtat ttcagctaag cctgctatgg ggccagaaga cataagtctt agatcgttag
    12901 caatttttat tagttcaagt gctaagtttc ttaatcctga agaaaaatta gatatatcga
    12961 atttgctttg gaggaaaaag tgaaggtttt cagatatttt taaatttaaa ccagaaatgt
    13021 taaaaagatg tttaacaatt ttttctcgat agcctttcgg tgtattaaca ccagttccaa
    13081 ctgctgttcc acctattcca atgaactcta attttctttg gctattgata atttcttctt
    13141 tagctctctc tagtgcgtat gcatagactt caaactcaga gcccaatgtt acaggaatag
    13201 catccatgag atgagtcctt ccaattttta taatattttt aaaatcaata gatttcattt
    13261 ttaaagatgc aatcattaca tcaagagatt ttataattaa attaatgtta attaatattg
    13321 caatattcat tgctgttgga aaagtatcat tacttgattg agacatatta acatgatcat
    13381 tagggctaac agtcatatag tcgcctttac tttttcccag aacttctaag gctcgatttg
    13441 cgattatttc atttgcattc atattaaaag cagtacccgc tcctgaatta attgcgtcta
    13501 caacaaattg atctaagaat tgtcctgata aaatttcatc gcaagcttga ataatcactt
    13561 tcgcaattct ctcatctaag acttgtaatt caaaattagt taatgctgct gattttttaa
    13621 tcataacata agcttttata aggtttatat gagatttatt tcccgttact ttgtactgtt
    13681 ctattgcgcg agctgtaaat ggaccataat atgcagtatc cgggacttta aattctccta
    13741 gtgaatcttt gtctattcta aatgacatat aatgactaca aaattcttat ttatataata
    13801 tgagttaact ataaaacaat cgatttaaaa taatgctgat ttctaaaatc attttttaaa
    13861 agtttatata atatatttat atagttcaca aaccgcatac caatatgccg caaactaaac
    13921 ccattgtaag tattgagaat gttgtcgctt ctgcaactat aaatcaaacg gttaatttaa
    13981 atcaaatcac acaaatattt cctgatgttg aatatcaccc agatcaattt ccgggtcttg
    14041 tttttagact aaaatctccc aaaactgcta ctttaatctt tagctcggga aaaatggtat
    14101 gtacaggagc caaatcagaa gatcaggcaa ttaaagcagt gaaaagtgtc gttcaaaagt
    14161 tgaaaaaagg aagcatacaa atcgcaaatg aacccataat tgaaatacaa aatatagttg
    14221 catccgcaag tttaggaggt aaaattcact tagaattagc tgctagggtt cttcctcgca
    14281 gtatgtatga acctgaacaa tttcctgggt taatccatag aatgttagat ccaaaaacag
    14341 ttatccttct gtttgcaagt gggaagttag tttgtacagg ggctaaaaaa gaagcagaag
    14401 tatatcgtgc tgtacataat cttcataatt tattagaaga aaaaaacctt atgatctatg
    14461 aagggtaatt atttttgtaa tgatagtttt ttttcatcat ttatccactg atttttatag
    14521 agaatatttg ccaactcgtt gatagttaat aaaaagtgta aatttattcc aattgcgtta
    14581 aaatttttta ttgtcgtatt gtcccctctt aacaatatac ataaaacatt ttcgactatt
    14641 cctccattat ttctaaggtt ttcaatagca atgtttaagg attgtcctgt agtaactaca
    14701 tcatcaatta ataatatttt ttgagtattt tccaaccttc cttcgactaa attattaatt
    14761 ccgtaaccct tcttttcttt tcttagataa attagtggtc gaaacatttc atatgctagt
    14821 gcagaagtaa aaattaaccc agaagtagga attgtagcaa gcatatcaaa tcggagattt
    14881 tttgattgaa taatatctct taatgcaatg attgtctgat gaaatattcc aggaaaactt
    14941 tgtattaacc ttaaatcaat gtaaaaacta ctatttttac cactcgctag tttaaaattc
    15001 ccaaacttta aagaattaga ttgtattagg aattctgcaa ggttagttgt ataactatcg
    15061 ttcatttatc tatactatga aaaagattta ttttaattct ttacaattat atctatgcca
    15121 gagatatggc ttggttatgg ttcaacagat gtagttttag atattaaaca agaaaatttg
    15181 ttgagctgtt atccaaagtt agatttgtta tcgaatgaaa atataaaaga aattctaact
    15241 aatataaaat tttcaaagaa aactctcatt tttccattat cctcttcaaa gtccatatta
    15301 aaaataatat cttttttgat agaaattaat aaaactgata tcacaaagga aattgaaatt
    15361 ggaacatttt ctgactcttt ccttcaaatt aaaaatcatt atgatgataa catcagaatt
    15421 ttccaaatgg atagaaacga tttttatgaa aaaatcaaaa aatttgataa tgttattttt
    15481 atttcaaaga tgggatatga acctcttttt ggattcagtg gaactccttc tatattgata
    15541 agaagttttc taaatggttt gatgaatgaa atttttcatt ctaacgaaat tatacgacct
    15601 aattctggtc aaataaggga accactcaaa atagcgttag agttttatga taaattaggt
    15661 tactattcta tcgaggtttt atctaatcaa tatggaattt ctcgtcttta ttatggattt
    15721 aattcatcat ttattaaatc cacaatttca gattttcaat caaatactaa ttttaatctc
    15781 aatgagcaaa agtcattaat acttagtgca ggtccagaac ctgagaatca tatcactctt
    15841 actaactcat tatattcatt atggaattct ataggagttt tgaaaaatca aggaacggct
    15901 gttttactag cagaaaatag ccatggtata ggtgagggtg ctttaactat gtatttagaa
    15961 ggtagattga atctttctga aatcaaaaaa ataaattatg taaatggatt agagcattta
    16021 gtttatcttg agaatttaaa aaatgaatat caccttggca tagtttcttc gataccgttt
    16081 cattatacta aaacaaaatt aggatttaac acttttaata atgttaaaga agttttacaa
    16141 aatatggtat tacgttatgg aaaatcaagt aaggttctga tttgtcctga tcctaatatt
    16201 acagttttta taaaacaata agaattatta taatatctga gataactttt atctatttat
    16261 tttaaaatac tttacgaatt tgttcttgta tataaagtta aataccatgt caaattaaat
    16321 atagaaattg caatctattc ttagaagtgt tggtggttat gttattcgta attgcgaaga
    16381 aaaagatctt ccatttgtta ttaatataaa catgataaca cttccagaac attattctga
    16441 ttattttttc acttctattt tagcagaatt acctgaagca tttattgtag cagagataaa
    16501 aaatcagata gttggatata ttatgtgtaa gatagaattt ggattct
//
LOCUS       DASVEV010000309         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_754099, whole genome shotgun sequence.
ACCESSION   DASVEV010000309 DASVEV010000000
VERSION     DASVEV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754099"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..748)
                     /locus_tag="VF222_13490"
     CDS             complement(227..748)
                     /locus_tag="VF222_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX7143484.1"
                     /translation="MQEKNMLNWNKVRPTWDEYFMLQAEIAKLRSNCLTRQIGAVIVR
                     DNRQIATGYNGTPPGILNCIDGGCKRCQERINGKLNSGVNLERCLCTHAEANAIMQCA
                     LFGNGSSTKDTTLYSTFAPCLECSKMCITVGIKRIVVLHDYPEDGNILLQEANIKLDK
                     LKRKEILPWCKFI"
     gene            864..1424
                     /locus_tag="VF222_13495"
     CDS             864..1424
                     /locus_tag="VF222_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143485.1"
                     /translation="MRSSLQKNDEKRARTVFLIVFYFCAITMSLITFYSLYVSIDEYL
                     RTGKVVIGEVLVNTEFPFPGLAKLVTYLMIVTVVGWYCVTKLGADKVKKIPESIISIF
                     QLIVLAIMVISLYEFIYNFVIWNSFITVDAINGIIRLDDINVPYPNPNTPWNPVFATK
                     MSLSALLISAHGFYIMSRHKSQDKVV"
     gene            complement(1457..>2808)
                     /locus_tag="VF222_13500"
     CDS             complement(1457..>2808)
                     /locus_tag="VF222_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7143486.1"
                     /translation="EISTMISDLKTLINIPSVSAKDQNLVECAEAVSALMNRVGIKSE
                     IIYLDPKDKNIVTSYLPPPIVYGEIKSQKNPNSKTILFYNHYDVQPEDPLDQWDVPPF
                     SGEVSGNYIYGRGSSDDKGELITRIKAVEYFLRETGDVPCNIKFIVEGEEEIGSIHIE
                     KYLTKYKEKFACDGIIWEFGYINEKNIPIISLGMKGLLYVELIACGPNKDTHSSLAVI
                     VENPAWNLIKLLNSMRDENGRILISDWYNDVRNFNQEEEEILNLELFEANEFKKEYGI
                     SRFLGDISGIDVKKALCGSPTCNIAGFVSGYVEQGAKTIIPSFAKVKLDFRLVPDMDP
                     QKQFAKLQQHIKDLGFKNININFIHGEAAARTSPNDPFVKIIKESAKRVFGDVILNVS
                     SAGTGPMHSFQKILKAPSVSIGSTYVYSRIHSPNEFARIDLLTNITKCIGEIMENYN"
BASE COUNT          919 a          436 c          384 g         1069 t
ORIGIN      
        1 atagtattgc taagagaatc caatatagtt tgtagttctt gttcatttat agaaagatca
       61 tatttatctt tagtcatatt tttctttatt cagtataaag tagtataaaa ttctttcatt
      121 atagaaaaaa ttactattat gagaggtagt aaaaatatat atatatttat attaacaata
      181 caccatttag caagatatag aaaaaaatat tattatagac aatttattag atgaatttac
      241 accaaggaag aatttctttt ctctttaatt tatcaagctt tatatttgct tcttgtaata
      301 atatgttacc atcttcagga taatcatgga gaacaacaat tcttttgatg cctacagtta
      361 tgcacatttt actacattct aagcaaggtg caaatgtact atacaacgtt gtatcttttg
      421 tgcttgaacc gtttccaaat aatgcacatt gcatgattgc attagcttca gcgtgggtgc
      481 ataaacatct ttctaaatta acccccgaat tcaattttcc attaattctt tcttgacatc
      541 ttttgcagcc tccatctatg caattaagta ttccaggagg agttccatta taacctgttg
      601 ctatctgcct attatctcta actataactg ctcctatttg tctcgttagg caattagatc
      661 ttaactttgc aatttcagct tgaagcataa aatattcatc ccaagttggc cgtactttgt
      721 tccaatttaa catatttttt tcttgcataa actaatcttg gatcttaact ataataatat
      781 aagcatgaat ataatattat aaattctaaa aatttttatg tgaattttta taactaagat
      841 caataaagta ggagtataat ataatgcgta gttctttaca aaaaaatgat gagaaaaggg
      901 caagaacagt ctttctaatt gtattttact tttgtgctat aacaatgtct ctaattactt
      961 tttattcttt atacgtaagc atagatgaat atttaaggac aggaaaagta gttataggtg
     1021 aagttcttgt caatacagaa tttccttttc caggattagc aaaattggta acttatttaa
     1081 tgatagttac tgttgttggt tggtattgtg ttacaaaatt aggggcagat aaagtaaaga
     1141 aaattcctga atcgataata tctatttttc aattaattgt ccttgctata atggtaatat
     1201 cgttatacga gtttatttat aattttgtta tatggaattc atttattaca gtagatgcaa
     1261 taaatggaat aataagatta gatgatatta atgtacccta tcctaatcct aatacaccat
     1321 ggaatccagt atttgctaca aaaatgtctt tatcagcttt attaatttct gctcatgggt
     1381 tttacattat gtctagacat aaaagccaag ataaagtagt ataaatttgg gaaaagaaat
     1441 tttattagtt tttctattaa ttatagtttt ccataatttc tcctatgcat tttgtaatat
     1501 tagtcaaaag atcaattctt gcaaattcat ttggcgaatg tattctagag tatacatatg
     1561 tactgcctat tgaaacagaa ggcgctttga gaatcttttg aaatgagtgc atcggtccag
     1621 tcccagctga tgatacattt aaaattacat ctccaaaaac tcttttagct gactctttta
     1681 ttattttaac aaatggatca tttggtgaag ttctagcggc tgcttctcca tgaataaagt
     1741 tgatatttat gtttttaaaa cccaagtctt ttatatgttg ttggagttta gcaaattgtt
     1801 tttgtggatc catatcagga acaagacgaa agtcaagttt aacttttgca aatgatggga
     1861 ttatagtttt agcaccctgt tctacatatc ctgaaacaaa accagcaata ttacatgtag
     1921 gagagccaca taatgctttt ttaacatcaa ttcccgaaat atcacctagg aatcttgata
     1981 tgccgtattc ctttttaaat tcatttgctt caaacaattc taaattaaga atttcttctt
     2041 cttcttgatt aaaatttctt acatcattat accaatctga tattaatatt cgtccatttt
     2101 catctctcat actattgagt aatttaatta agttccatgc tggattttcc acaattactg
     2161 ctaaactgga atgagtatct ttatttggac cacaagcaat caattcaaca tacagtaaac
     2221 ctttcatccc taaacttatg atgggtatat ttttttcatt tatatatcca aattcccata
     2281 taattccatc acaagcaaat ttttctttgt atttggttag atatttttct atatgtatgc
     2341 ttccgatttc ttcttctcct tctacgataa atttaatatt gcatggaaca tcacctgttt
     2401 ctcttaggaa atactctact gcttttattc gagtaattaa ttcgccttta tcatctgatg
     2461 aacctcttcc ataaatataa tttccagaaa cctcaccact aaaaggagga acatcccatt
     2521 gatctaaagg atcttcaggt tgaacatcat agtgattata aaataagata gttttgctgt
     2581 ttggattttt ttgtgatttt atttccccgt atactatagg aggaggaaga taagaagtta
     2641 caatattttt atctttagga tcaagataaa taatttctga tttaatccct actctattca
     2701 taagagcaga aactgcttct gcacattcaa caagattttg atcttttgca gaaacactag
     2761 gaatgttaat caatgtctta agatcagaga tcattgtact aatctctg
//
LOCUS       DASVEV010000310         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1339134, whole genome shotgun sequence.
ACCESSION   DASVEV010000310 DASVEV010000000
VERSION     DASVEV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339134"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VF222_13505"
     CDS             complement(<1..818)
                     /locus_tag="VF222_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143487.1"
                     /translation="MSKGITITSTIFGMFLLTLMATGIQIPTFSVFAQESEIESVNAS
                     NSGSGNATSAQETDIETVNATSSPSSNATSSESSNATSSESSNATSVAKAAQAKPDAA
                     TISAGESFAQQGTVTSTQDPLPGHEAHQLALILPPRDDGAIYQGTLTYTASKPVEVVI
                     LQNFANETAVDPTYGGIATAPLGEGTVAISLLTPVYTGPINAASIPFAGNALALHTLN
                     GEPFAATYTVTGDVAQAQTFNAVSPPPLAEATQAETGDGKDGDGKDGDGKDGDG"
     gene            complement(968..1144)
                     /locus_tag="VF222_13510"
     CDS             complement(968..1144)
                     /locus_tag="VF222_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143488.1"
                     /translation="MPILLQLICDQCKKVILEKMGEKNLTLERFPITSQEAEKLNKEH
                     RGHECHIEAVEKIS"
     gene            1222..1371
                     /locus_tag="VF222_13515"
     CDS             1222..1371
                     /locus_tag="VF222_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143489.1"
                     /translation="MDFKICGYCGKKFFELGWPHIEGDSNRGVLKIEHFCSEEHKESF
                     LQSKE"
     gene            complement(2034..2675)
                     /locus_tag="VF222_13520"
     CDS             complement(2034..2675)
                     /locus_tag="VF222_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX7143490.1"
                     /translation="MLLRFERNRTSTIVIMYSLYLYILGLSLRNTSKALDIFDDEKRS
                     HIAIWNGIQRFGSSQIYKRKRISAFIVDETIIQIGNHHFWLWIAIAPVNRTILGIYIS
                     EERNMFVAENFIRSLVSKYGKHTVYTDGNTWYPQACNFLHLKHRLHSPLKKNLIERVM
                     QYFKDRTECFDDYYPCTKKINCDLSNVYNWLNLFTYAYNTKIKNTVPFLNGGK"
     gene            complement(2741..3034)
                     /locus_tag="VF222_13525"
     CDS             complement(2741..3034)
                     /locus_tag="VF222_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143491.1"
                     /translation="MFNADSGKSSSNKKKFHKINLGIRQTVLERAHYRCQGCSIKFNT
                     QETPFFSHINGSLKDNSPANLKALCKKCYVKTGENKIKDNMVGKIRSLIRRVI"
     gene            complement(3104..3490)
                     /locus_tag="VF222_13530"
     CDS             complement(3104..3490)
                     /locus_tag="VF222_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7143492.1"
                     /translation="MLNNLIAIVVISFIIVGSLWGIGNLLNPVEDHSMNSEGILLITE
                     NNFFNNTNPTLHINRNIPEKITIVNKDFVRHDFVIDELNVNTGYLFSGQDFTTAIASS
                     ESGAFEYYCSLHPSTMRGKLLISEKK"
BASE COUNT         1199 a          594 c          591 g         1326 t
ORIGIN      
        1 ttaccatctc catctttacc atctccatct ttaccatctc catctttacc atcacctgtt
       61 tctgcctgag ttgcttctgc tagtggtggt ggacttactg cattaaatgt ttgagcttga
      121 gcgacatcac cggtaacggt ataagtagca gcaaatggct caccatttaa agtgtgtaac
      181 gcgagagcat ttcctgcaaa tggaatggaa gctgcattga ttggtccagt atatacgggg
      241 gtcaagagac taattgcaac tgttccttct cccaatggag cagtagcgat accaccataa
      301 gttggatcta cggcagtttc atttgcaaag ttctgtaaaa ttacaacttc aactggttta
      361 cttgctgtat atgtaagtgt accttggtat attgcaccgt catctcttgg aggtaaaatt
      421 aatgctaatt gatgagcttc atgccctggc aaaggatctt gtgtagaagt cactgttcct
      481 tgttgagcaa aagattcacc tgctgaaata gtagctgcat cgggtttagc ttgtgctgct
      541 ttagccacgc tggtagcatt agaggattca ctgctggtag cattagagga ttcactgctg
      601 gtagcattgg atgatggact gctggttgca ttcacagtct caatatcggt ttcttgagcg
      661 ctagtagcgt taccagaccc agagttggaa gcattcacag attcaatctc actttcttga
      721 gcgaatacgg agaaggtcgg aatttggata cctgttgcca taagagttaa taaaaacatg
      781 ccaaatattg tagaggttat tgttatacct ttagacatga aactatgcgg aatacataga
      841 atttaaagtt tcctatataa ctttcttttg aagaaaaatc aatcagacta ttctattact
      901 ttacacattt gaggatcgaa aaatggaact caaatatatt gctcataaaa atatcatgat
      961 aaaaatctca actgattttt tctacagcct ctatatgaca ttcatggcct cgatgttctt
     1021 tatttaattt ttctgcttct tgactagtaa ttgggaaccg ttcaagggta aggttctttt
     1081 cacccatttt ttctaaaatg actttcttgc attgatcaca aattaattga aggagtatag
     1141 gcataatgaa ataatatata ttataaaata tattggttat taaaaaaaag gattttattt
     1201 catttttaag aaatattata aatggacttt aagatttgcg gttattgtgg aaaaaaattc
     1261 ttcgagttag gatggcccca tattgaggga gattctaata gaggtgttct caagatagaa
     1321 catttctgtt ctgaagaaca taaagaatca ttccttcaat ctaaagagta agcgtaaatg
     1381 atattaattt taagaatcgc atttggctgt gttaatttaa cgaaacagta ttagctgtga
     1441 atgttccatt atagcatatg ttgttacgat ttgttagaaa tagaacatcc acaacaatta
     1501 tagtcatgta tatatttttt ctatttgtat ttatttggtc tcagcctccc aaatacatct
     1561 aaagcactta taatttttag tggtgagaat taaaggagtc atgatgctgt attgggttga
     1621 ttcaaagatt taattgattc cctacataca aaaaccaaaa gaatagcatt ggctcaaatt
     1681 tattttactc catatcatac tatagcaata attgtgactg tctataataa aataataaat
     1741 tatgaacatt tttagggtat ttcatcaacc tttatattaa atccaaaagt aaaacatatt
     1801 aaagaaaaaa cacattcaac actattttcg agtaaatgta ctaacgttaa tgtgataatg
     1861 tgatagtgat gttatggtag tgattttcgg attatgttac ttttagtttc aaacctattt
     1921 tactcaatat cttactatag caataattgt gactgtcata caaaatcatt tttagcgatt
     1981 ttcatcaaca tttttatgaa ctataggtaa atcaggtcct gctaatttaa cattcattta
     2041 cctccattga ggaatggaac tgtatttttt attttggtat tgtatgcata ggtaaacagg
     2101 ttaagccaat tgtatacatt tgacagatca caatttattt ttttagtaca aggatagtaa
     2161 tcatcaaaac attctgttct atccttgaaa tactgcatta ccctttcaat tagattcttt
     2221 ttcagtggtg aatgtaatct atgttttaga tgcaaaaagt tgcatgcttg aggataccat
     2281 gtattaccat cggtatatac tgtatgttta ccatacttgg agacaagcga tcgaataaag
     2341 ttttctgcta caaacatgtt tctttcttca gaaatgtaga ttccaaggat agttctatta
     2401 actggtgcaa ttgctatcca taaccaaaag tgatgattcc caatctgaat gatagtttca
     2461 tctactatga atgcagaaat tcttttcctt ttgtagattt gagaagaacc aaacctttga
     2521 atcccgttcc atatagcaat atgacttcgt ttctcgtcat caaatatatc taacgctttg
     2581 gaggtattcc gtagacttaa accaagaatg tatagataaa gagaatacat aattacaata
     2641 gtggatgttc tattcctttc aaatcttaat aacattcaag tttgaatagg acatccacaa
     2701 ctaatactgt ttcgttaaat taacaggtcc tcgcattttg tcaaattact cgtctaatta
     2761 atgacctaat tttacctacc atattgtctt tgattttatt ttctccggtt tttacataac
     2821 actttttgca aagagcttta agattagcag gactattatc ttttaatgat ccgttaatat
     2881 gtgaaaaaaa tggagtctct tgtgtattaa atttaatgga acaaccttga caacgataat
     2941 gcgctctttc aagtactgtt tgtcggattc ccaaattaat tttatggaat ttttttttat
     3001 tactgctact ttttcctgaa tcggcgttaa acatggaaga tcatattatc cattccttag
     3061 aattaacttt ttctatgaac aatacaaatt ttaagacaaa tgattatttc ttctcagaaa
     3121 taagaagttt tcctcgcatt gtagatggat gcagggaaca ataatattca aatgctcctg
     3181 attcgcttga tgcgatagct gtggtaaaat cttgaccgga gaaaagatat cctgtattaa
     3241 catttaattc atctataaca aaatcatgtc gaacaaaatc tttatttaca atagtaattt
     3301 tctcaggtat gtttctatta atatgaagcg tagggtttgt attattaaaa aaattatttt
     3361 ctgttattaa taaaattcct tctgaattca tactatggtc ttctactgga tttagtagat
     3421 taccaattcc ccataatgaa cctacaataa taaaagaaat tacaactatt gcaattaaat
     3481 tatttaacat gacacttata tttgatatcc aaaagtttat gttattaaaa tcataaaaaa
     3541 tcaacaaaca ttatatcata tctgaacaaa ttttatgttc catattttat tccttttgtg
     3601 gtaattgatc gaaggcattc aataacagat aatatagcta gataacttgt ttttggatta
     3661 gtagaactag gaatattttt tacacgtact attaattcgc caaattttcc
//
LOCUS       DASVEV010000311         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_950168, whole genome shotgun sequence.
ACCESSION   DASVEV010000311 DASVEV010000000
VERSION     DASVEV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950168"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            968..1303
                     /locus_tag="VF222_13535"
     CDS             968..1303
                     /locus_tag="VF222_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7143493.1"
                     /translation="MTSIMLSIGLLANPTITPVFSQEGSVSIAPGAANPNNEQSFDPS
                     QISVPTGSTVFWTNDDSIQHTVTSDEGLFDAGPISPGDTFENTFDSAGEFDYHCAIHP
                     FMTGVITVG"
     gene            complement(1393..1797)
                     /locus_tag="VF222_13540"
     CDS             complement(1393..1797)
                     /locus_tag="VF222_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143494.1"
                     /translation="MSERNDNTTKFLHAFTQSVLNSDRSIRWVAITDQDGNIINEQNR
                     EEGFKSLLTKEENQESAINTIIRQKTRTKFEPKIGKLSYALGRYQKLSRCLIPINENY
                     YLILTMDFDQYDFDKIIMEKIIPLIKQEKEKF"
     gene            1841..1981
                     /locus_tag="VF222_13545"
     CDS             1841..1981
                     /locus_tag="VF222_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143495.1"
                     /translation="MSDSNVIPPKSSSSEDEYYKQWIKNRILEEAYNRDKWANRDNSS
                     NK"
     gene            2018..2200
                     /locus_tag="VF222_13550"
     CDS             2018..2200
                     /locus_tag="VF222_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143496.1"
                     /translation="MYPALGDLELIRREARGMDRKEDPFIKLVGTSNEDTITITDRGR
                     TYYEENKNTEIAQLDF"
     gene            complement(2230..2472)
                     /locus_tag="VF222_13555"
     CDS             complement(2230..2472)
                     /locus_tag="VF222_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143497.1"
                     /translation="MSNSFELLTGPIDAIQKKYEILSDIVTEYNGKVHGSQRDRDPKT
                     GLIDLLVYYEVPLGQREEVKRKYDNFVRNIQMVLKQ"
     gene            complement(2512..2718)
                     /locus_tag="VF222_13560"
     CDS             complement(2512..2718)
                     /locus_tag="VF222_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143498.1"
                     /translation="MSSNYNINPKPCVYNCNTQIYWNTSVNEYWEVFTKKKHICPNRV
                     NKPVTTTSNNVAGTVARTNTKPTY"
     gene            complement(2715..3119)
                     /locus_tag="VF222_13565"
     CDS             complement(2715..3119)
                     /locus_tag="VF222_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143499.1"
                     /translation="MTNRNWDEDEDFIGESIYETQGSRLRAIARIIKKYLQIKGVESN
                     PKTVHDAMAPIINMVLNDLEFRKFTLVSYKIFIFYDEKNTEFYIKRITDEMLELIKEE
                     NIDNLDSKELENKINELESSLNKKDKNNKNTK"
BASE COUNT         1111 a          491 c          438 g         1280 t
ORIGIN      
        1 aatacaacat aaacaaaagg ggttttttca tcttgagaag cctcaatttt tgatactaca
       61 aattcatttt tcatagacaa tattattatt aactatactt ataataatcc ttagtttgta
      121 aattgtctta atcatttata acaaagaata tgagttataa ataaaggtca atcgtcatat
      181 tatttgtctt tattttaatc atctttatta tctgtatatt gtaactatta gcagcattga
      241 atagaaatat gaattgtgat atagacctaa agataaatta atacatgaag ataaacatta
      301 aattttaatt agaaagtata caatacacta catttgatgg ataaaggttc gagtcccaac
      361 tcaagaatta atttatctca ggatgttata cattatagtt tttctaggat tgaaatttaa
      421 atcaaaaaat attttgttaa tccataatgg ttttattatt aattatatta atcataaaaa
      481 aataaaataa gcattgttca gtgtaatgaa gttaataaaa aataagaaat attattttat
      541 aatatagacg ttgacagaaa aagtgtcaac tcatacttac aatgttctat tttgaattta
      601 atatcaaaaa attttatatt catttacaat acataaaaat ataggtatat ctaagaacta
      661 ttttacgttg caaaaaaatt atgattatac tcctacttat tctttagcag ttttaaatat
      721 tttttctaat tcactataga actctttgtt tacagcagac tttgacagag atgcttgcca
      781 ttgttttgct attccatcat agaattgaag gaaaatctta ttcctattgt agtgatgtta
      841 aaagtggtgg ttgttgaaat catgccaatt attttaaatt aaaagttaaa ttaattaaat
      901 ataattctta atatattaaa cttcaccatc gcatacaatg aaaactacta tttcgagatt
      961 tatactaatg acaagcataa tgctatccat tggcctttta gcaaatccaa caataacacc
     1021 tgtcttttct caggaaggtt cagtatctat agcacccgga gcagccaatc caaacaatga
     1081 acagtcattt gatccttctc aaatcagcgt gccaacagga agcacagttt tttggacaaa
     1141 tgatgattcc atacaacaca ctgtaacttc tgatgaagga ctatttgacg caggtccaat
     1201 atctccagga gacacatttg aaaatacatt tgattcagca ggagaatttg attatcattg
     1261 cgcaatacat ccattcatga caggtgtgat tacggttggc taataattct tattaataat
     1321 aaatagacaa tcctattttt ttcacaataa acatctatca attagttcgt ggaataaaat
     1381 taattcttct gattagaatt tctctttttc ttgtttaatt aatggaataa ttttttccat
     1441 tattatttta tcaaaatcat attgatcaaa atccatagtt aaaatcaaat aataattctc
     1501 attgattgga atcaaacacc tactcaattt ttgatatcgt ccaagtgcat aacttaattt
     1561 tcctattttt ggttcaaatt tagtccttgt tttttgtcta attattgtat ttatcgcaga
     1621 ttcctggttc tcttcttttg taagaagtga tttaaagcct tcttctctat tttgttcatt
     1681 tataatattg ccatcttgat cagttattgc tacccatctt atagacctgt cagaatttaa
     1741 tacactttga gtaaaagcat gaaggaattt cgtagtatta tcatttcttt cagacatatt
     1801 tgtatatttt aatataatct tatttccttg tattaaatac atgtctgata gtaatgtaat
     1861 tccaccaaag tcaagttctt cagaagatga atattataaa caatggataa aaaatagaat
     1921 tttggaagaa gcatataatc gtgataagtg ggctaataga gataatagtt caaacaaata
     1981 gtcttcaaat aacattacaa taaatgaaat tcaagaaatg tatccagcat tgggagattt
     2041 agaattaatt agaagagaag caagaggaat ggataggaaa gaggatccat tcatcaaact
     2101 tgtaggcacc tctaatgagg atactattac aataactgat aggggtagaa cctattacga
     2161 ggagaataaa aatacagaaa ttgctcaatt agatttttaa cgattatttt agtttataaa
     2221 ttaatttctt tattgtttta ataccatttg aatatttctt acaaaattat catacttcct
     2281 tttcacttct tccctctgtc ccaaaggaac ttcataatat accagcaagt caatgaggcc
     2341 agtcttagga tccctatctc tttgtgagcc atggactttt ccattatact ctgtcacaat
     2401 atcagataat atttcatact tcttttgtat ggcgtcaatt ggaccagtta gtaattcgaa
     2461 tgaattggac atctttgatt ttggttggtt tgaattccat ggttttttgc tttaatatgt
     2521 gggtttagta ttagttcttg ctacggttcc tgctacatta ttggaagtag tagttactgg
     2581 tttgttgact cgattaggac aaatatgttt tttcttggta aatacttccc aatattcatt
     2641 tactgaagta ttccaataga tttgggtatt acaattataa acacatggtt ttggattaat
     2701 gttgtaatta gaactcattt cgtgttcttg ttattcttat cttttttgtt tagacttgat
     2761 tctaattcat tgattttgtt ctcaagttct ttactatcca aattgtcaat attttcttcc
     2821 ttgattaatt ctagcatttc atctgtaatt cttttgatat aaaattccgt gtttttctca
     2881 tcataaaaaa taaagatctt gtaactaact aatgtaaact ttctaaattc caaatcgttc
     2941 aataccatgt ttataattgg agccatagca tcgtgcacag tttttggatt agattcaact
     3001 cctttaattt gaagatattt tttaattata cgtgcgattg ccctaagacg gcttccctgg
     3061 gtttcgtaaa tgctttcccc tatgaaatct tcatcttcat cccaatttct attagtcatt
     3121 tttttattat tgtacccagg aaagttttag tttattagta attcgatttt attttatttc
     3181 ttccaattca atagtaatca gtaattgctt atcgtctagt tctttaacat tatctaaata
     3241 atttttagga atccaaatca cacgattatc tttcattcgg tttaacttgc cagcaaattt
     3301 aatcttgatt ttgtctccca
//
LOCUS       DASVEV010000312        17008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_370024, whole genome shotgun sequence.
ACCESSION   DASVEV010000312 DASVEV010000000
VERSION     DASVEV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370024"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF222_13570"
     CDS             <1..234
                     /locus_tag="VF222_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143500.1"
                     /translation="AGWYYKRRERSYLNTSMNRIDFTYDALKKDKLEGVKQLEEIRKE
                     ITNLFKKGKLTDSHYNILDKRISELINTLLRKE"
     gene            477..1085
                     /locus_tag="VF222_13575"
     CDS             477..1085
                     /locus_tag="VF222_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7143501.1"
                     /translation="MLSVIVINKSNDIYIDNLINFDKSLFIIINEFHVKLPAELNYFM
                     ILITLYGRELFLPFVLGLIFIFGGKNGKKAVVIAAISILILIPIVSITKDYIERPRPF
                     VPGLDTLMSVDTSNYSFPSGHSTLIVAGFVTTLVLFKGNKYTKILPILLVIDAGLVCF
                     SRIYVGVHYPFDVLGGFFLGSGISFLIISLVNYIQKAKTIRR"
     gene            complement(1117..1248)
                     /locus_tag="VF222_13580"
     CDS             complement(1117..1248)
                     /locus_tag="VF222_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143502.1"
                     /translation="MFGSNITINLIIQEEEEEEDDMILMMITYSFLVNFNFKFNLNL"
     gene            complement(1254..1910)
                     /locus_tag="VF222_13585"
     CDS             complement(1254..1910)
                     /locus_tag="VF222_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX7143503.1"
                     /translation="MDNIFFSIPLLLHVLDLFGVMVFAVTGALKAIQYKMDMVGVIIL
                     ASSTGLAGGLIRDLLLDRLPPKIILDPYYLTIAVATGIIVFFLHPFFKEREILFLKFD
                     AIGLGVFTIIGSSIAYEMLGLNFLAMSLAGIFTAIGGGIVRDVFVKEIPLVFIKELYA
                     SASFLGIIIFFILLYFDFNYYISMVVCIVIITVFRLLAIKYNWNLPTKIIFKKKRDRE
                     "
     gene            complement(1996..2391)
                     /locus_tag="VF222_13590"
     CDS             complement(1996..2391)
                     /locus_tag="VF222_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143504.1"
                     /translation="MPKPGFKSITVSENIYKKFFNVYEKRKNDLELKGITSFSGYLTS
                     MMEEMMLKHEAFAKQAPFMEKISIDSDRVLIKDNKRNRIAEVILRNGELRCLLDDKID
                     CVHIGFVYSIPEIYIVLNNKGMKAPKSLH"
     gene            complement(2729..3562)
                     /locus_tag="VF222_13595"
     CDS             complement(2729..3562)
                     /locus_tag="VF222_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7143505.1"
                     /translation="MKLSFSSNAFKKFSLDNAIREIAKIGYKGVEILCDIPHAYPPEF
                     SEEQTKKIKNLISDLDLGISNLNAFTLYAIGDVYHPSWIEADNKLREIRVKHTIDCIY
                     LAKKLGAKNLSTEPGGPISIQKTEVGSHVLLKIFAEGLKKVKNIAEENNVKILVEPEP
                     FLLIENSEQFLQLMKTICSDYIKLNFDIGHFYCVKEDPSETILKLIDYIEHFHLSDIA
                     YNRVHYHLIPGIGSINFEQVFDTIKKIGYKGFVTVELYPYQDNPIYVAKQAYNYLKVF
                     I"
     gene            complement(3603..4424)
                     /locus_tag="VF222_13600"
     CDS             complement(3603..4424)
                     /locus_tag="VF222_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7143506.1"
                     /translation="MSGFFDPHIHMYSRTTDDYDAMSKAGIEVIVQPSFWLGGPRTFV
                     GTFIDYWEHLISFETTRAKQFGIEHFVCISVNPKESIQRPLALDALQAMLKFIDRERV
                     VAIGEIGYNLINELEEEIFIKQLDIASTRKIPIMIHLPHQNKTKGIERIEDILNQNGN
                     KYDRKKILIDHNTEETIKKTLEMGLWAGLSVYPLTKLSPKRAIDIIRKNGPDSIMINS
                     AADWGISDPLSVPLVAREMRKEGFTKNEIEKVTMFNAYDFYKQSNKFTWTPEIQC"
     gene            4804..5763
                     /locus_tag="VF222_13605"
     CDS             4804..5763
                     /locus_tag="VF222_13605"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX7143507.1"
                     /translation="MKNNSSSSTTNIKSFYKSSNMLSIDLAGIHLRNPLLLASGFLGI
                     SQEIFNRLYNDGLGAIVSKSISVSPLDGYKGPTVVSLGEKGYLNAVGLANPGSDVFAH
                     EIINNQVPLIVSVVGSSQSDFPKLISKFDKLNILGYEINLSCPHVAKMGMEVGDDHDL
                     VTKIIKTIKSKTKKPVIIKVGIGNTDVLKLATIIQESGADAITAINTIRAMAINVETG
                     MPILSNKVGGLSGIPLKSIGVRCVFEISKKVNIPVIGCGGIFTWEDAVEYILAGATAI
                     ELGSVIGFEGLKAFNQIKLGITRYLEKKDCKNIKEIIGLAHKY"
     gene            5747..6577
                     /locus_tag="VF222_13610"
     CDS             5747..6577
                     /locus_tag="VF222_13610"
                     /inference="COORDINATES: protein motif:HMM:NF000796.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX7143508.1"
                     /translation="MHTNIDKIRTVVIEEVIEETPSVKTFVFNDRLSSNGKPGQFLMV
                     WIPRAEELPMSIMISNKKDHAAITIRKHGFGSTSLFEKKRNDLIGIRGPYGNYFSVSK
                     SLKSPLLIGGGTGLVPLMRLATIFSKMQGRCTILMGAQTQKEILFKGLAEEILSKIRT
                     NIIVTTEDGSYGIKGVVTDALKDILKKEQFDMIYTCGPELMMMSIYNLSVTHSIPIQA
                     SLERYMKCGIGICASCCINDKLVCKDGTVFNENQLSELSEFGKVFRDKSGRPSYYQFN
                     "
     gene            complement(6594..8081)
                     /locus_tag="VF222_13615"
     CDS             complement(6594..8081)
                     /locus_tag="VF222_13615"
                     /inference="COORDINATES: protein motif:HMM:NF038049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelD-related putative sulfur metabolism protein"
                     /protein_id="HEX7143509.1"
                     /translation="MNTDFWENIYKYKEYGFEPLNWLSTFSNEIESDILEFSLNKIKG
                     TNWKITPDWFDSFYKSERKNPEVTRRIYNLSDSDLQQQSETGIKRSLAIIQLNKEIAD
                     NQEKRLNLLSNLINSIKSKCITKKISAINTSSNKQFALLDFIETRRGNKVGYIIANNY
                     ITQVTDVLCSPESDIHCKITVAGVIENLNLLGCIDEFKIFPIYDAPKEEMLDATRQNL
                     DSITLSNNIFMEDYSSLKIGNLFFGASATATTYREIPTRYTEIEEEMNVIVTDKFGSI
                     IPLSLFLLTKINEENISIFEKHGIFINELEKEKNNIINNLSHPRFELGKLINKYCPDF
                     GGKFDKGSHITAVFPILTQGILALKKLVDYIDMEIIIDKIPIKNKEMSSYAVKEYFVQ
                     HSTSSFNGCHLIIASRECTDSIIQDLKKIHLEPEVIGKVGKKGKPLLSIKDMEIDKYI
                     LRRSKIYKKIRSFRAKGFENIDKDGRRSSSPLSSSSSLATTSEKN"
     gene            8464..9270
                     /locus_tag="VF222_13620"
     CDS             8464..9270
                     /locus_tag="VF222_13620"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX7143510.1"
                     /translation="MIESNCEHSIDDDNFTFLSNASQRSKQNFVIDNLAKRFEKKSAT
                     DILKWSIEKFGNRIALASSFGAEDVVIIDMMAKIDKSKTKILTLDTGRLNQETYDVMD
                     EIRQRYGISIESYFPNSQQVEEMVRTKGFNLMYESIENRKLCCEIRKVNPLNRALASL
                     DGWITGLRREQALTRLNIKKIEIDPTHNNILKINPIADWSEDMVWEYIKENQVPYNKL
                     HDMGFPSIGCEPCTRAISKGENRRSGRWWWEESSQKECGLHIDHSITKKK"
     gene            9297..10541
                     /gene="sat"
                     /locus_tag="VF222_13625"
     CDS             9297..10541
                     /gene="sat"
                     /locus_tag="VF222_13625"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX7143511.1"
                     /translation="MINARDNRNISRVNNTINGLKDKKLKNTFIQNNMIKKIPEPHGG
                     RLVNRIFESKNNNTKDMFSIEIDDNLRNDVENIGDGIFSPLEGFIGKDDFESVLKSGR
                     LKNGLPWTIPIILDVEEPTAKKIQDYREISLKNKGEHFATLYFDEFYSYNKLEMVRAL
                     YLTDDEQHPGVSKTFKMKEYLVSGRIDVCKKINNFKLRNYRLEPIKTREEIERRGWKT
                     IVGFQTRNIPHIAHEMLQKAALNIYDGLFLNPLIGKKKIGDFKDELILESYLKLIENY
                     YPQERVMFVTLHTEMRYAGPKEAIHHAIMRKNFGCSHFIVGRDHAGVGDYYSPFAAHE
                     IFKEYPDIGIEPIFFPSFYYCKKCLGYANERNCPHGSKYKEELSGTRMRKMVTSGEMP
                     GKHLMRPEIADVIIKFPDPFVS"
     gene            10729..11451
                     /locus_tag="VF222_13630"
     CDS             10729..11451
                     /locus_tag="VF222_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143512.1"
                     /translation="MKFSSFVCAFIFLILFSYNYLYAQNESEAESIEENLDVTKSKSH
                     ERFVVPFSASNTDRKNPVIHTYEIPNDVSWILGIENNMTYNNSSEAKTIIKLMENQPS
                     EKFIELAMFEQNEKFWVAVNTNESGYIRVHEQDTDGWSRDEPIMVAHANNQGLTITNG
                     KRIVIDKLSLDGFSLGSIAIYGKDDTDSPINTLDGYLSFDLLYGSPRDSPLYFLPIVM
                     LVLVGGIVFYLLKVKKRDRDDE"
     gene            complement(11458..12174)
                     /gene="nth"
                     /locus_tag="VF222_13635"
     CDS             complement(11458..12174)
                     /gene="nth"
                     /locus_tag="VF222_13635"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7143513.1"
                     /translation="MKKKSSIQLQNNNNSRELIKVEYILNKMETTLKESENLSRLTAL
                     KELQMEEDNDPFKILIGTILSARSRDENTSKIVRKLFQKYKNALELANADLNDIKSII
                     HSIGFYNIKAERIKQVSKIILEKFHGVVPNEIEDLLGLPGVGRKTANCVLVYAFNKPA
                     IPVDIHVHRISNRIGIVKTQNAKKTEEELIKIVNKEYWLILNNIFVIYGQNICLPIRP
                     KCQMCSLKGICNFYTNQKKI"
     gene            complement(13023..13187)
                     /locus_tag="VF222_13640"
     CDS             complement(13023..13187)
                     /locus_tag="VF222_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7143514.1"
                     /translation="MLKIRKEIECYDKVMEIRPNYPKIWYNLGLGYKQKAKEYVHRSE
                     AINKIRRKTK"
     gene            complement(13439..14101)
                     /locus_tag="VF222_13645"
     CDS             complement(13439..14101)
                     /locus_tag="VF222_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143515.1"
                     /translation="MDNHFSNNWIKEKKRNSTDILKDSLRSQQPLKPKMELAKNKIQI
                     QNKKLDSMLEKLKAREKMYFEQVVSSLQKHDSQQGKMISTELAQVKKTAKTMSQIKLA
                     LEQVHMRLESTIDIGDSMAALGPAMGALSHIKGKINAVLPEADGELNEINTTFNDILT
                     NASGIGSNTFDFNNFGEDVGRILDEASFIAEQRTGMDIPDIPEEEGTNLRKSRSVREH
                     SH"
     gene            14374..15852
                     /locus_tag="VF222_13650"
     CDS             14374..15852
                     /locus_tag="VF222_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143516.1"
                     /translation="MNILQWCDIDECLLIANTDEVYKKYAGLKHSNESILSLANLRLL
                     NSELFLKNYREPFDLFLSVVENFVDSSTKDLELKFHDIRTKKIVSKYKFNNKNINWNT
                     WRLFNNNEKNSQKRKKVFDEFIEKTKYISPVIYSRFEKIRSLYSTIQQSSDSNISIGS
                     NNLDPLSGYLMNEKITYTKLLNFVKYLGNNSSKPFKKSLQKVSAEILKRDTEYYDDFY
                     FFRNVIYKDFNKTFKNINPLKEVKKILKVLNFDLNRISFDTEYRQNKYPSPICFFVRI
                     PYDIRVLYKSESPYFDLQGCFHESGHAMHASSINSEKEYWKKYHISMGVVEIFSILLE
                     RLTKKRIFLKSSLGITDDNLLNELESRNNFMELFFVTFYSANALMKASFWKDRLSIED
                     SNSLYSKLVKDFTGIEIPGAYWMLHHILPQAIMYVPSYLFAAVRAKELEVHLQNTFGE
                     KWWKDKESGKYLEQIMSPGAEIDLSVFSKLDSNLYLKEIISV"
     gene            16553..16912
                     /locus_tag="VF222_13655"
     CDS             16553..16912
                     /locus_tag="VF222_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143517.1"
                     /translation="MKREFIASRIEATQDGSPYVYVTFSDPHDYKPGSDKRMNPFGPN
                     VMAFTSPEDLMKNLPKAMGDISKMISGGMGGGAGGVTGDNPTFKITMKDYEDMNIKVG
                     DKVTIEIKKSDSGSIYT"
BASE COUNT         5963 a         2345 c         2537 g         6163 t
ORIGIN      
        1 gcaggttggt attataaaag aagagaaaga agttatctaa atacaagcat gaatagaata
       61 gacttcactt atgacgcctt aaaaaaagac aagttagaag gagttaaaca attggaagag
      121 atacgaaaag aaataacaaa tctatttaaa aaaggaaagc tcacagattc acattataat
      181 attcttgata aaagaatctc cgagttaatt aatactcttt taagaaagga gtgatatatt
      241 ttatgtaaga actatttaat acaaaatcta aacaatttat tttttattat agtttaaaaa
      301 tataacgtca taattctttg ttcgatgcta caatctggtg catatttata aaatagtcct
      361 tgataataat aataataatc atcatcatca gaatgttatt attaacctaa agaaatttat
      421 attaaagcat gaataaaata ttttttttaa caattttttg ctttgggtca tttatattgt
      481 tatcagttat agtaattaat aaatcaaatg acatatatat tgataatctt ataaattttg
      541 ataaatctct ttttattata ataaatgaat ttcatgtaaa attacctgcg gagctgaatt
      601 actttatgat tttgataaca ctttatggga gggaactttt tttaccattt gtacttggac
      661 taattttcat atttggaggt aaaaatggaa agaaggcagt agtcatagca gcaatctcaa
      721 ttttaatact gattcccatt gtttctataa ctaaggatta tattgaaaga ccaagacctt
      781 ttgttcctgg tttggataca ctaatgtctg ttgatactag taactattca tttccctctg
      841 gacattctac tctaatagtt gctggctttg taactacctt ggttttattt aaaggaaata
      901 agtatactaa aattctgcct attcttttag tcattgatgc aggattagta tgcttttcca
      961 gaatttatgt tggagttcat tatccatttg atgttttagg gggttttttt ttaggaagtg
     1021 gaatatcttt tttaataatt agtcttgtaa attacataca aaaagctaaa actataagaa
     1081 ggtaactaaa aagttgaagg aatttgaaat gacattctat aaatttaaat taaatttaaa
     1141 attaaaattt acaagaaaac tatatgttat catcatcagt atcatatcat cttcttcttc
     1201 ttcttcttct tgtattatta aatttatagt tatattagat ccaaacaaat cttttattct
     1261 ctatctcttt tttttttaaa tatgattttg gtcggtagat tccaattgta ttttattgct
     1321 aagagtcgaa atactgtaat gataacaata cagacaacca tggaaatata gtaattaaaa
     1381 tcaaagtata gcagaataaa aaagatgatt attcctaaaa aacttgctga ggcatataac
     1441 tctttgatga atactaaggg gatttcttta acaaaaacat ctcttactat tcctcctccg
     1501 atggcagtaa aaatacctgc taaagacatg gcaagaaaat ttaatcccaa catttcgtaa
     1561 gcaatactgg agcctatgat agtaaagact cctaaaccta ttgcatcaaa ctttaaaaat
     1621 aaaatttcac gttccttaaa aaagggatgt aaaaaaaata caataatgcc tgtagctaca
     1681 gcaatagtta gataataagg atctaatata attttaggtg gtaatctatc caaaagaaga
     1741 tctctgatta atcctcctgc taaacctgta ctgctagcta atattataac tcccaccatg
     1801 tccattttgt attgaattgc tttgagagcc ccggttacag caaataccat tacaccaaat
     1861 aaatctaata cgtgaagtaa taagggaatt gagaaaaaaa tattatccaa ctataaatat
     1921 tagttatgtc ttaatattta tttaaattga ttaattttct cttacatatt aaaacaattt
     1981 aattataaaa atattttaat gcaatgattt tggagctttc attcctttat tgtttaatac
     2041 aatgtatatc tctggaatag aataaacaaa tcctatgtga acgcaatcta ttttatcatc
     2101 tagtaaacac cgtagttccc catttcgtaa tatcacttct gcaattctat ttcttttatt
     2161 atcttttatt agtactcgat cagagtcaat tgaaattttt tccataaaag gagcttgttt
     2221 agcaaatgct tcatgcttta acatcatctc ctccatcatg cttgtaagat atcctgaaaa
     2281 acttgtaatt ccttttagtt ctaaatcatt cttccttttt tcgtaaacat taaagaattt
     2341 tttatagatg ttttcactaa ctgtaattga tttaaaacca ggcttaggca ctcttatacc
     2401 gtattagacc gtaacatata agacttacta tataaattta gataaatatt gacttggatg
     2461 ggctatttat ttttattttt ttattatcat ttcccgtatt actattatta atgaaataaa
     2521 ttcatattta tatttgttat agtttatagt agttatatca tataagaaat atcacttgtt
     2581 atatattaac aaaaatgtgt tcgaactgtt tagttagttt atttattaat tttattttat
     2641 tatgtttaga aaacaaagat ctaagaatac gattgacata tatatatcaa agtttgaatc
     2701 acaaaaccat ggattatttg ataaatattt aaataaatac ttttaaataa ttatatgctt
     2761 gttttgctac atagatggga ttatcctgat atggatataa ttctacagta acaaatcctt
     2821 tatatccaat cttttttatt gtatcaaaaa cttgttcaaa atttatagag cctattccag
     2881 gtataagatg ataatggact ctattataag ctatgtctga taaatgaaaa tgctctatgt
     2941 aatcaattaa tttcaatatt gtctcagaag gatcttcttt gacacaataa aaatgcccaa
     3001 tatcaaaatt taattttata taatcagaac agatagtttt cattaactgt aaaaattgct
     3061 cagagttctc aattagcagg aaaggttctg gttcaactaa tatttttaca ttattttctt
     3121 ctgcaatatt cttgactttt tttaacccct ctgcgaatat ttttagtaaa acatgtgatc
     3181 ctacttcagt cttttgtatg ctaattggac ctcctggttc agttgaaagg ttttttgcac
     3241 ctaatttttt ggctaaataa atgcagtcta ttgtatgttt aaccctaatt tctcgtaact
     3301 tattatcagc ttcaatccat gaaggatgat aaacatctcc tatcgcatat aatgtgaatg
     3361 cattaaggtt agaaattcct aaatctaaat ctgaaattaa attttttatt ttttttgttt
     3421 gttcttctga aaactcagga gggtaggcat gtggtatatc acataaaatt tctacccctt
     3481 tataacctat tttagctatc tctcttatag cattatcaag agaaaatttc ttaaaagcgt
     3541 ttgaactaaa ggagagtttc atattaaagt aatcaagtaa tgattatatt ctatttaata
     3601 ctctaacact gaatttcagg agtccatgta aatttatttg attgtttgta aaaatcatat
     3661 gcattaaaca tggtaacttt ttctatttca ttttttgtaa atccttcttt tctcatctct
     3721 ctagcaacta atggcacgct caatggatca gaaatccccc aatcagctgc actatttatc
     3781 attatactat ctggtccatt ctttcttata atgtctatcg ctctctttgg agaaagtttt
     3841 gttaaaggat aaaccgaaag accagcccac aatcccattt ccaatgtttt ttttattgtt
     3901 tcttccgtat tatgatcgat cagaattttc tttctatcat atttattacc attttgattt
     3961 aatatatctt caattctttc tattccttta gttttatttt gatgaggtaa atggatcata
     4021 atgggaatct ttctggttga tgcaatatca agctgtttga taaaaatttc ctcttctaat
     4081 tcattaataa ggttgtaccc tatttcacca attgctacaa ctctttctct atctataaac
     4141 tttaacattg cttggagtgc atctaacgca agtggtcttt gtatagattc tttaggattc
     4201 acagaaatgc atacaaagtg ttcaattcca aattgtttag ctcttgtagt ttcgaacgat
     4261 atgaggtgtt cccaataatc aataaacgtt ccaacaaaag tccttggacc tccaagccaa
     4321 aaagagggtt gtactatgac ttctattcct gctttagaca tagcatcata atcatctgta
     4381 gttcgagaat acatatgaat atgcggatcg aagaatccag acattacttg taaaattata
     4441 agttatatta aatatatatc ttgtaacatt ctcaacaaaa ttttaggatg ctatatgtgt
     4501 tattatttgt tgttgatcat agcaataact agttctttta ctttattgta tattttattt
     4561 ttagtaaaaa tttataggaa acattatgta tgttaaatat ttaattctga tatatttatt
     4621 gtctaatata aagtaatata taaagacatt tacatattga tactttgata tttaaaaaaa
     4681 atcaaaaagg ggaatgtttg aatataacta gaatgttttt attacaattt aatatatttt
     4741 aatgctgaaa taaatctaaa acgaacaact aataagatgt taaatgatta aaacaactag
     4801 aaattgaaga ataattcaag ttcaagtact actaacatta aaagttttta taaatcatct
     4861 aatatgttat ctattgatct tgcaggaatc caccttagaa atccacttct tcttgcatca
     4921 ggatttttag gtatttctca ggaaattttt aacagattat acaatgatgg tttaggggct
     4981 atagtaagca aatcaataag tgttagtcct ctagatggat acaaaggacc gaccgttgtt
     5041 tccttgggcg aaaaaggtta tctaaatgct gtaggacttg caaatcctgg atctgatgta
     5101 tttgctcatg aaattatcaa taatcaagtt cctcttatag ttagtgttgt cggatccagt
     5161 cagtcagatt ttccaaaact aataagtaaa tttgataaat tgaatatctt gggttatgag
     5221 attaatctct cttgtccaca tgtcgccaag atgggcatgg aagttggtga tgaccatgat
     5281 ttggtaacta aaataattaa gactattaag tcaaaaacca aaaaacctgt aattataaaa
     5341 gttggaatag gaaataccga tgttttaaaa ctagcaacga taatccaaga gtcaggagct
     5401 gatgccataa ctgcaattaa tactattaga gcaatggcca taaatgtaga gacgggcatg
     5461 cccattctga gcaataaagt gggcggatta tctggtattc cattaaaatc cataggagtc
     5521 agatgtgttt ttgaaatatc taaaaaagtc aatattcctg taattggatg tggtggaatt
     5581 ttcacatggg aagatgcagt agaatacatt ttggcaggtg caacagctat agaattaggt
     5641 tctgtcatcg gatttgaagg gttgaaagca ttcaatcaaa taaaattagg cattacaaga
     5701 tatttggaga aaaaagattg caagaatata aaggagatta ttggtcttgc acacaaatat
     5761 tgataaaata cgaactgttg ttattgaaga ggtaatagag gaaactcctt cggtaaagac
     5821 ttttgttttt aatgataggt tatcttcaaa tggaaaacct gggcaatttt taatggtctg
     5881 gatcccaagg gcagaagaac ttccaatgag tataatgatc tccaataaga aagatcatgc
     5941 tgctattaca atacggaaac atggatttgg atccacctct ctttttgaaa aaaaacgaaa
     6001 tgatctaatt ggtatacgcg gaccttatgg taattatttt agtgtaagta agagtttaaa
     6061 aagtcctttg ttaataggag gtggaactgg gttggttcca ttaatgcgtc ttgctactat
     6121 atttagtaaa atgcaaggaa gatgtacaat acttatgggc gcacaaacac agaaagagat
     6181 actgttcaaa ggtctcgcag aagagatctt aagtaaaatc aggactaaca taattgttac
     6241 tactgaggat ggtagttatg gaataaaagg agtggtaaca gatgctttaa aagatatttt
     6301 aaagaaagaa caatttgata tgatctatac ttgcggacct gaacttatga tgatgagtat
     6361 ttataattta tcagttactc attctattcc aattcaagct agcctagaaa gatacatgaa
     6421 atgtggaata gggatttgtg caagttgttg tataaatgat aaacttgtct gcaaagatgg
     6481 tactgtgttt aatgagaatc aattatctga attatcagaa tttggaaaag ttttcagaga
     6541 caaatctggc cgtccttcgt attatcaatt taattaataa ttaatatttt aaactagttt
     6601 ttctcagatg ttgttgctag actagaagag gaagagaggg gtgatgacga ccttctgcca
     6661 tctttgtcta tattttcaaa tccttttgct ctaaacgacc taattttttt atatattttt
     6721 gatcttctaa gaatatattt gtcaatctcc atgtccttaa tactcaaaag tggtttgcct
     6781 ttttttccaa cttttccaat aacttctggt tcaagatgta tttttttcaa gtcttggatt
     6841 atcgagtcgg tacattccct ggaagctatt atcaagtgac agccattaaa tgaagaagta
     6901 gaatgttgta caaaatattc tttaacagca tatgaagaca tttccttatt tttaatagga
     6961 attttatcta ttattatttc catatctata taatcaacta actttttgag agcgagaatt
     7021 ccttgggtta agataggaaa aactgcagta atatgtgaac ctttatcaaa ttttccccca
     7081 aaatcaggac aatatttgtt tattagcttt cccagctcaa atcttggatg gctcaagtta
     7141 ttaatgatat tatttttttc cttttcaagt tcattaataa atataccatg tttttcaaaa
     7201 atagatatgt tttcctcatt aatttttgtt aacaaaaata aacttaaggg aattatagag
     7261 ccaaatttgt cagtaactat aacattcatt tcctcttcta tctccgtgta tcgtgtagga
     7321 atttctctgt atgttgtagc agtagcacta gctccaaaaa aaaggttacc gattttcaag
     7381 gaactataat cctccatgaa gatattatta gataaagtaa ttgaatctaa attctgtctg
     7441 gtagcatcaa gcatttcttc ttttggtgca tcatagatag gaaaaatttt aaattcgtct
     7501 atacatccta gtaaattcag attttctatg actccagcta ctgttatttt gcaatgaata
     7561 tcagattcag gagaacataa gacgtcggtt acctgtgtaa tataattatt agcaatgatg
     7621 tatcctactt tatttcctct tcttgtttca ataaaatcta acaatgcaaa ctgcttgttt
     7681 gaagatgtat ttattgcact aatttttttg gttatacatt ttgatttaat actatttatt
     7741 aaatttgaca gaagattgag acgcttttct tgattgtctg cgatttcctt attcaattga
     7801 ataattgcta gtgatctttt gatacctgtc tcactttgtt gttgtaggtc cgagtcggat
     7861 agattataaa ttcttctagt aacttcagga ttctttcttt cacttttgta aaacgaatca
     7921 aaccagtctg gtgtaatttt ccaattcgtt cctttaattt tatttagaga aaattctaat
     7981 atgtcagatt caatttcatt agaaaaagta gaaagccaat ttagtggttc aaaaccatat
     8041 tctttgtact tgtaaatatt ttcccaaaaa tctgtattca atataatcta tgtaatgatt
     8101 tatagtaatg atattaaaat atagatattt tttattagtt ttatttaatt tatatcggaa
     8161 atgtaagtca taacattatc atcatagggc ataaatttat aaacaaagat atttacaatt
     8221 ttttaatata ctccaaagta gtatttacaa atgtttttgt tttatttctc tataataaga
     8281 ctttccaaag ctaatttatt ttatatctaa ttttcaagac aacacttgac atcctttttg
     8341 caaataatag tgataataat ttttatccat ttactattct caaagattaa gctcgttaaa
     8401 tttgatatct aactttagca tctcaaaata aatatataca aatattaaat aaattaaatt
     8461 attgtgatag aatctaattg tgaacacagt atagatgatg ataattttac atttttaagc
     8521 aatgcaagtc aaagatctaa gcaaaacttt gttatagata atttggccaa gagatttgaa
     8581 aaaaaatctg ctacagatat tcttaaatgg tcaattgaaa agtttggaaa tagaattgca
     8641 ttagcatcaa gttttggtgc agaagatgtt gtaataatag atatgatggc caaaatagat
     8701 aaaagtaaaa cgaaaatttt aacattggac acaggtagat taaaccaaga aacctatgat
     8761 gttatggatg aaatacgtca aagatatggt atttctatag aatcatattt cccaaattca
     8821 cagcaagtcg aagaaatggt acgtacaaaa gggtttaatc ttatgtatga aagtatagaa
     8881 aatagaaaat tatgctgtga aataagaaag gtaaatccac ttaatagagc tctagcatct
     8941 cttgatggat ggataacagg tctaagaagg gaacaggctc ttacccgctt aaatattaaa
     9001 aaaattgaga ttgatccgac tcataataat attttaaaga ttaatcccat tgctgattgg
     9061 agtgaagata tggtttggga atacataaag gaaaaccagg ttccatacaa taaactacat
     9121 gatatgggat tcccaagtat tggatgtgaa ccatgtactc gtgctataag caaaggagaa
     9181 aatcgtagat ctggcaggtg gtggtgggaa gaatcgtctc aaaaggagtg tggacttcac
     9241 attgaccata gtataacaaa aaaaaaatga atttaatata acaataatca agaatcttga
     9301 taaatgcaag agataatagg aatatatcta gagttaataa tacaattaat ggtctaaaag
     9361 ataaaaaatt gaagaatact tttattcaaa acaatatgat aaaaaagatt cctgaaccac
     9421 atggcggtcg acttgtaaat agaatttttg aatcaaaaaa taacaatacc aaggatatgt
     9481 tttccatcga aattgatgat aatcttagaa atgatgttga aaatattgga gatggtattt
     9541 ttagtccgtt agaaggtttt attggaaagg atgattttga aagtgtttta aaatctggaa
     9601 gattgaaaaa tggattgcca tggacaattc ctataatctt agatgtagaa gaaccgactg
     9661 caaaaaaaat acaagattat cgtgaaatat cactaaagaa taaaggtgaa cattttgcaa
     9721 ctctatattt tgatgagttt tattcctata ataaattaga gatggttagg gcattatacc
     9781 taaccgacga cgaacaacat cctggagtat ctaagacctt taaaatgaaa gaatacttag
     9841 tttctggaag aattgatgtt tgtaaaaaga taaataactt caaattacga aattatagat
     9901 tagaaccaat aaagactaga gaagaaattg aaagaagagg atggaaaact atagttggtt
     9961 ttcagactag gaatatcccc cacatcgcac atgaaatgct tcaaaaagct gctctaaata
    10021 tctacgatgg attattcctc aaccctttga ttggaaaaaa gaaaataggt gattttaaag
    10081 acgaattgat acttgaatca tacctaaagt taatagaaaa ttattatcca caagaaagag
    10141 tgatgtttgt tactcttcat accgaaatga gatatgcagg acccaaagaa gcgattcatc
    10201 atgctattat gagaaaaaac tttggctgtt cccactttat agtaggaagg gatcatgctg
    10261 gtgtaggtga ctattacagt ccctttgcag ctcatgaaat cttcaaagaa tatcctgata
    10321 ttggaattga acccatattt tttccatcat tctattactg taagaaatgc ttaggatatg
    10381 ctaatgaaag aaattgtcct catggttcca aatataaaga agaattatct ggaacacgaa
    10441 tgagaaagat ggtcacatct ggagaaatgc ctggaaaaca tttaatgagg ccagaaattg
    10501 cagacgtaat tataaaattt ccagatccat ttgtttcttg attttattaa tatctgacaa
    10561 acaatcgttt tgatattctt ttagttattt tactaaaaaa gttggtgagg attgattgta
    10621 ttaactaact ttattgttct cctgttttta atccatgatt tacgttcata ttactctaaa
    10681 taattatatc aaatgcgatc aatgtatgca agttctatat tatatttttt gaaattttct
    10741 tcatttgttt gtgctttcat atttctgata ttattttcat ataattatct atatgctcaa
    10801 aatgaatctg aagctgaatc tatagaagaa aaccttgatg taacaaaatc aaaaagtcat
    10861 gaaaggtttg tagttccctt tagcgcatcg aacacagacc gtaaaaatcc agtgatccat
    10921 acttatgaaa ttccaaatga tgttagttgg attcttggta ttgaaaataa tatgacctat
    10981 aacaattctt ctgaagcaaa aactattatt aaattgatgg aaaatcaacc tagcgaaaaa
    11041 ttcattgaat tagccatgtt tgaacaaaat gaaaagttct gggttgctgt taataccaat
    11101 gaatctggtt acataagagt ccacgaacaa gataccgatg gatggtctcg tgatgaacct
    11161 attatggtag cccatgctaa taatcaaggt ttaacaatta ctaatggtaa aagaatcgtc
    11221 attgacaagt tatccttaga tggttttagc ttaggttcta ttgctatata tggaaaagat
    11281 gatacggatt ctccgataaa cactttggat ggctatcttt cctttgatct tttatatggt
    11341 agtcccagag attctccatt atattttcta cctatcgtta tgttagtttt agttggaggg
    11401 atagtattct accttctcaa agttaagaaa agagaccgtg atgatgaata aatttcatta
    11461 aatttttttt tgatttgtgt aaaagttgca tatacctttt aaactacaca tttggcattt
    11521 aggtcttata ggaagacaaa tattttgtcc ataaattaca aaaatattat ttaaaatcaa
    11581 ccaatattct ttgtttacta tttttattaa ttcttcttct gttttttttg cattctgtgt
    11641 tttaacaata ccaattctgt tagaaattct atgaacatga atatcaactg gtatggctgg
    11701 tttattaaaa gcatatacca aaacgcagtt tgcagttttt cttcctacac caggtaatcc
    11761 taaaagatct tcgatttcat ttggaacaac tccatgaaat ttttctaaaa tgatcttgga
    11821 tacttgttta atcctttctg ctttaatatt gtaaaaacct atactatgta taattgattt
    11881 tatatcattc aaatctgcgt tggctaattc taatgcattc ttatatttct gaaaaagctt
    11941 acgtacaatt tttgacgtat tctcatctct tgaacgagca gataaaatag tgccaattaa
    12001 tatcttgaat ggatcattat cttcttccat ttgaagttct ttgagggctg taagtcgtga
    12061 taggttttca gactccttca atgttgtctc cattttatta aggatatatt ctacttttat
    12121 cagttctcta gagttattat tattctgaag ttgtatgctt gattttttct tcaatgttta
    12181 ttatttacta gtaggccaag tgtaataaaa aaattatcta gatgtgtgtt tattttgtga
    12241 ttttatgaat tacaacttat ttaatatatt gaaatatatt actgacctta atgaattatg
    12301 acaacacttc gagattttac gaattacaag tttcacagaa tatttcttat tgatctatga
    12361 tgagatttca aattaacctt atgggtatag aattacttta ataaatagtc ataggactag
    12421 taaatttgaa attgctaaat cagatttaga atcataagaa ttgttgaaat cacaaagcag
    12481 taacttttag ctttttatct gattatataa aaacgagtga caaaataatc ataataaatt
    12541 tttttcacaa atagaaaata cttattcata ctgtaccagc tttttaaaag aagttcatct
    12601 atgagttcat aagtaaagaa aagtcctggc taatgttagt aaagaaacgt aaaaggtata
    12661 atgatcaaag atggacatgg gaaataggga gagattaatt tgcggtgaga cctattacta
    12721 atttattaaa aaaagaaagt agaaaaaaac taaatataga atatttcatt taataaaaaa
    12781 acaataccca aattaattta gggcttctcc ttttaccaac acaaaagata ttcgctaaca
    12841 aaactatttt gaaattttta aacctaattc gattcaaata ttattattat taatgggaga
    12901 atcgcaaaaa gaatgtaaaa accggtctcc tcattttttc ttttatttga tttttcaatt
    12961 gcgtctaatc ttttgatagt tcaattagtt tatctggtaa atgtgtatac tattatcttc
    13021 tttcattttg tctttcttct tattttgttg attgcttcac ttctatggac atattcttta
    13081 gccttttgtt tatatcctaa accaagatta taccatatct tgggatagtt agggcgaatt
    13141 tccatcactt tatcatagca ttctatttct ttccggattt ttaacatatt gatacaatct
    13201 cactattcat caaaatgatt catgtttttc tttaacatgt cattcatcca ttcttacaac
    13261 tacaaatttg attattgtaa taaggaaatt tttgatattt aaactctgag tgtgaatatt
    13321 ctactatgat aataaagtca tggaagataa aagaggtaat tgaatattac atcttatttc
    13381 tttaatttaa taaaaaaagg aatgttggat taatattatc atacatgtga tgataatgtc
    13441 agtgagagtg ttctcttact gaacgtgatt tccgtaaatt tgttccttct tcttctggta
    13501 tgtctggtat atccatgcct gttctttgct ccgcaataaa gctagcctca tctaaaattc
    13561 taccaacgtc ttctccaaaa ttattaaaat caaatgtatt actccctatc ccactggcat
    13621 tagttaaaat atcattaaat gtcgtgttta tttcattcaa ttctccatcg gcttctggta
    13681 aaacggcatt tattttacct ttaatgtgag agagagcacc cattgctggg cctaaagctg
    13741 ccattgaatc gcctatgtct attgtagatt ccaatctcat gtggacttgt tctagagcta
    13801 attttatttg cgacattgtt ttagcagtct ttttaacttg ggcaagttca gtagaaatca
    13861 tttttccctg ttgtgaatca tgcttttgta aagatgaaac tacctgttca aaatacattt
    13921 tttcacgtgc ttttagtttt tccaacattg aatcaagttt tttattttgt atttggattt
    13981 tatttttagc taattccatt ttaggtttta gaggttgttg agatcgcaaa ctatctttca
    14041 atatatcagt agaatttcgt tttttttctt taatccaatt attgctaaaa tggttatcca
    14101 tgaatactga tactattcta gttcagacct taaactacaa tgtcatcttt gaataactaa
    14161 ctagtaattt tttataaaca tactccacac agaaattgca ggcataagat gtcatcaatt
    14221 gtaattatta atttatatta tataacttaa gttggtcaaa atgtgtagtc agataatatt
    14281 ataaatgaga ttacaaaaaa actgaattct tataagttaa agcagcttga ttatatttat
    14341 ataaaccgat ttactattta ttttgttttt taaatgaata tcttacaatg gtgtgatatt
    14401 gatgaatgcc ttttaattgc aaacactgat gaggtttata aaaaatatgc cggtttaaaa
    14461 cattcaaatg aatcgatact atctcttgca aatttaagat tgttaaactc agagctattt
    14521 ttaaaaaact atagagaacc atttgacctt tttctttcag tcgtagaaaa ttttgtagat
    14581 tcttcaacta aagatttaga gcttaagttc catgatatta gaacaaagaa aattgtttct
    14641 aagtataaat tcaataataa gaatataaat tggaatacct ggaggctatt caacaataat
    14701 gaaaaaaatt ctcaaaaaag gaagaaggtt tttgatgagt ttatagaaaa aacaaaatat
    14761 atttctcctg ttatttatag tcgctttgaa aagatacggt cactttattc aactattcaa
    14821 caaagtagcg acagtaatat ttctattggg tccaataact tagatccatt atcaggttat
    14881 ttgatgaatg aaaaaataac atatacaaaa cttttaaatt ttgttaaata cctgggtaat
    14941 aattcaagca aacctttcaa aaaatcactt cagaaggtgt cggctgaaat tttaaaaagg
    15001 gatacggagt attatgatga tttttatttc tttcgtaatg taatctataa agattttaat
    15061 aagactttta aaaatattaa tcccttaaaa gaagtaaaaa aaattctaaa agtattaaat
    15121 tttgacctaa atagaattag ttttgataca gaatatagac aaaataagta tccctcacca
    15181 atatgtttct ttgttagaat accttatgat attcgtgttc tgtataaatc tgaaagccct
    15241 tattttgacc ttcaagggtg ctttcatgaa tcaggacatg cgatgcatgc aagctctata
    15301 aattcagaaa aagaatactg gaaaaaatac catatttcca tgggagtggt cgaaattttt
    15361 tctatattgt tagaacgttt aacaaaaaaa aggatttttc ttaaatcctc tcttggaata
    15421 actgatgata acttattgaa tgaattggaa tcaagaaata acttcatgga attatttttc
    15481 gttacctttt actctgcaaa tgctctcatg aaagcaagtt tttggaaaga tcgtctaagt
    15541 attgaagatt ctaattcgtt atattctaaa ttagtcaaag attttacagg aatcgagatt
    15601 cctggagctt attggatgct acatcatata cttcctcaag ctataatgta tgttccaagt
    15661 tatctatttg cagcggttag agctaaagaa ttggaggttc atttacaaaa tacctttggt
    15721 gaaaaatggt ggaaagataa agaatcaggg aagtatttag agcaaataat gtctccaggt
    15781 gcagaaattg atctttcagt attttcaaag cttgatagta atttatacct aaaagaaatc
    15841 atatctgttt aataattact aaatgttatc tttatcaatg tcttggaatc gtcataaact
    15901 actttaagta agaattacat tatatgaaaa aaggtcaatg ctaattattg tatgaacaat
    15961 atgtgttttc tctgtatgta taaaacaaat tatactttgt tatgatctca tctatattcc
    16021 tatcaaaaag ggtatattat ttaaaataat tattaattag ttgaataatt ttttgcatgc
    16081 gattataaag aattaaaatg ctatagtgga taaagaattt gattttgtct tttgtaagat
    16141 taaaaacatt actgctcact aaaatgacta aattatagtt gatataacat tgtctaaaac
    16201 ttacaaattt ttaaagtttc gagatcgtta ttttaggtga taagtaggat tttaaaatat
    16261 taatattata ataatagcta acagaaatat ctatttctat taatattttt tacacttagt
    16321 attcatttac tatttttacg tgatactatg attttctaaa ttagggtata caagagccta
    16381 tactaattct aatacaagaa gtatgtgtgg atttatgcta ttataagagg gtacattcta
    16441 tagatcatgt aatttgtaca acatgattat gatctctttc taaagctagc ttgatagaga
    16501 ctgctaatat atccaaaatt ataaattgaa ataactatca ttttacttta gaatgaaaag
    16561 agaatttatt gcctctagaa ttgaggccac acaagacggt agtccttatg tttatgtaac
    16621 attctcagat cctcacgact acaagccagg aagtgataaa agaatgaatc cttttggtcc
    16681 taatgttatg gcgtttactt ccccggaaga tttaatgaaa aatttaccaa aggcaatggg
    16741 tgatatatca aagatgattt ctggaggtat gggtggaggg gcaggaggag tgacaggaga
    16801 taatcctacc tttaagataa ctatgaaaga ttatgaggat atgaatataa aagtcgggga
    16861 taaagttact atagaaataa agaaatctga ttcaggaagt atctatacat aaccaatcaa
    16921 ctttaataat tttttatctc ttatatgttg taatgaactt acaaataata ttgtaaatga
    16981 tgtacgaaca gtagattata attgcaac
//
LOCUS       DASVEV010000313         7037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_565954, whole genome shotgun sequence.
ACCESSION   DASVEV010000313 DASVEV010000000
VERSION     DASVEV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565954"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF222_13660"
     CDS             <1..429
                     /locus_tag="VF222_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143518.1"
                     /translation="RDNRTGTQGPQGPQGSAGPQGPAGPQGIQGIPGLLGLQGPSGIT
                     QLNATNVYSVDNSTTSTTTGNFAITPAVCDPGDFVLNGGYNLFGSIIEVNDTISTFVD
                     QSISFPTLGEGWVAIVFVNDEASSITLNVNALCFDNPPLR"
     gene            888..1148
                     /locus_tag="VF222_13665"
     CDS             888..1148
                     /locus_tag="VF222_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7143519.1"
                     /translation="MKYRSRTEIVGNILDAANGGATKTKIMYIAFLSYAQLKEYLSIL
                     VENNLIEYLDGNRKFKTTEKGLNYLKMNNEIGELLQTITIKN"
     gene            2019..2708
                     /locus_tag="VF222_13670"
     CDS             2019..2708
                     /locus_tag="VF222_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143520.1"
                     /translation="MYSNVPSVSRAIKDILFSNSIYINSLKLGIANYTALAIKIKPEV
                     DKMIGSEVNINTIVVSIKRLADALQQNQELESITQEEKENLAGARISLTGSMLDVEFD
                     KEMENIEKVFELFDRESDIRFNIFQSKNHMNLFIENISEIKKIFSNHSQTINPSKIKE
                     GVSMITISLPWEESELRKTYQLLSMISTILYDNQVFLHNAFFTPDEIVLIINEIDAAK
                     VYELLRVKFHR"
     gene            3109..3927
                     /locus_tag="VF222_13675"
     CDS             3109..3927
                     /locus_tag="VF222_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143521.1"
                     /translation="MFNIELLASLLQRDKMIRSAVLMLTYRNIVAVVDKIFIIWQILF
                     PLVYIFVAGYAYSSLITENEIVIGGKTIPYNVFLALGMIGFNVMNSSTVAGSIIWNDK
                     RNGMLHQILVMPYSKLQYIASTLLTIIVMGLASAAIILIAGLPIIAGNITIDITSTIY
                     LVFALIMGSIFFGSIAIIISTRLKSNEGFTVIINSVFLFFAFASSTFYPPHGLPEPMQ
                     YAFYVNPLTYIADIIREGMFSAVDTFTNIKILVLGVISGIAFLIATFSMYKMRI"
     gene            complement(3937..4941)
                     /locus_tag="VF222_13680"
     CDS             complement(3937..4941)
                     /locus_tag="VF222_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7143522.1"
                     /translation="MSNTVNNNNNNNQQHCISVKNLVKSYDSSNLKRNVIDDISFDID
                     YGTVFGFLGPNGAGKTTTIKILTGILEPTAASYIKILEKDIKKNNFKEIKRRIGVVTQ
                     NPSFEMNLTVERSMELYGFLWGNSSNKNKDKVRELLDIFELTSIKEIRNEDLSIGQRR
                     RVQIAREFLHDMDLLFLDEPTVGLDPRARRNLLNYIKKQVKCGLTVFFTTHIMEEVEY
                     LCNQICIINKGKIIEFDTPQILKQKYGSTKKIEIILQNPISKYIHDYFQKLSANSIDI
                     ENIENTENTFSITSNNPQNVLLAIVNFLFQNNIQIENISLNDPSIEEVFLKALKNNNT
                     "
     gene            4949..5077
                     /locus_tag="VF222_13685"
     CDS             4949..5077
                     /locus_tag="VF222_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143523.1"
                     /translation="MTTTITYIRTPIIILIDYIPAILFEKDSAIFLESKDDDYIKM"
     gene            5173..6693
                     /locus_tag="VF222_13690"
     CDS             5173..6693
                     /locus_tag="VF222_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143524.1"
                     /translation="MSLDFKKMIESVLQKKPDISAEDVRELIDEKKRKIGAGYLTDQG
                     ALFLVAADLGISFENNQSTQTSIKDLYIGANDVNLSARIINIYPVRKYTRRDTSEEVQ
                     NRTLTIYDKDSSIKLRLWDQHVSFPEDYDVKPGDLIKISHGYTKSGLDNKAIINLGSK
                     GIIEKSSSTTNDDNLEIPSLDAISKTVDEIKEPVDNIIVTGVLSSNPRVSRYVNFRNE
                     QGKSLHIPLSNDDGTKTVRSIIWNIDEDKIPGIFTQGSKVKLIGVKVKQGNPQYSNEE
                     FEIHGDEGTIIQVQQIKRVGEEGEGIDAEGEGEEEFDGSKVVVLKILSTGTEFESSVN
                     ENFLALDKENKNFISLVVDPKLIEEKIYPGIIIEGIPNRVFGNVMYFTDESSYIRIVT
                     ESYESAFPQDSEIETKIKNIQVSEKPYIVEAIVLQAPNTSNITTKSGEDITVSETLIG
                     DDTGEIRLTGWRDQSSKISKLNVGDRVKIIGAVAGMGRENRIEVTVKPFTEIRELE"
     gene            6825..>7037
                     /locus_tag="VF222_13695"
     CDS             6825..>7037
                     /locus_tag="VF222_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143525.1"
                     /translation="MLITNDLNLKVNSLNNNEAKFLIEILQLESANALSSKGVGGLEK
                     VSNEALQPLKAGDNKDLKNTTLQTTGL"
BASE COUNT         2533 a          982 c         1082 g         2440 t
ORIGIN      
        1 agagataata gaacaggaac acaaggtcca caaggtccac aaggttcagc aggtccccaa
       61 ggtccagcag gtccacaagg aatacaaggc attccaggtt tactaggtct acaaggaccg
      121 tcaggtatta ctcaattaaa tgcgacaaat gtctattcag ttgacaattc tactacatcc
      181 acaaccacag gtaattttgc tattacaccc gctgtttgtg atcctggtga cttcgtatta
      241 aatggaggct ataatctatt cggatctatt attgaagtga atgatacgat ttcaaccttc
      301 gttgatcagt ctatatcttt ccccacacta ggagagggat gggtagcaat agtatttgtt
      361 aatgacgagg cttcctcaat aacactgaat gtaaatgcat tatgttttga caatccacca
      421 ttaagataga cctgaactac caattttttt atttttattc tattaattag acaataataa
      481 tattccttaa tacttattga ttagaaaaat tatgtcacag aacaaatttg gtaaaaatat
      541 ttgtatttaa atttctaatg ataagggata tataaaaggt tacagaacca tactcctact
      601 tacagaataa aatcgaaact tacaaatatt tatgagaatt ctaactatat ctaaaaatac
      661 cttttaattt taataataat tatattttgg attatatata aattaaatga tatttaaaat
      721 tcaatcatat gtcgtatgaa ttgttaatat gattagtacg ataattattt catgatacaa
      781 gattagaatc aagcaatgaa ggaatttctt tagaatgctt ctcctataat tttcttagcc
      841 cttcttttga tattatatta atataactga tatacgtgta taataatatg aaatatagaa
      901 gtaggactga aatagtgggt aatatactag acgctgctaa tggaggagca accaagacaa
      961 aaattatgta catagctttt ctaagttatg cacaattaaa ggaatatctt tctattttgg
     1021 tggaaaataa tctgatagaa taccttgatg ggaatagaaa attcaagact acagaaaaag
     1081 gactaaacta tcttaaaatg aataatgaaa ttggagaatt gttacaaact attacaatca
     1141 aaaattgatt aattaattca aatttttttt tatactaaac atataataat attaattttt
     1201 ataaaaattc tatattaata tctattttaa cattactgaa aaataaacct ttaaatttat
     1261 atcttattac tataacccta tcgaattcag ttaccttaat attccaaaat caatatcaaa
     1321 ttatgtttct aataataaag ctaaataaac aagtcttcgg atcttagtaa atttaggatc
     1381 tctccaattt tttgtgccta ctaatatgct tattatcata taaaaaactt tatctaaagt
     1441 aaattattgt tattgaagta ttataaatca atatttaaaa taatgttact taatataaaa
     1501 aaattctatt gttgccaatt aaatatcata tatcaaagaa tttaagaatc aataatgtat
     1561 atgtctagtg aagataatct tcttcttttg ttcctcacta atcacttaat aggattgaga
     1621 ttacttactc taaccattaa tcaatttata gtggtttaaa gctatcaaat tgattgatat
     1681 gtcctcttta gtgtatacct attgtggtca tgctattttg tgtaagataa agagatacgc
     1741 taaattctcc cccccccctt tctaatcatt atttcgcaga tgaaaagaac tataattaat
     1801 cacatagata aataaagtat gatcaagtct tggtaaagca attaacatat attcttatta
     1861 tctgtcaaga aaaaaattgc aaaccaatct gccatagctt tttctattct tgtttgctcc
     1921 ttccataatt gctgtggctt ttatctcttc tacaaatatt cttatttttc atttaatata
     1981 taagaatgta atacctattt cattatgtaa taaagaaaat gtattccaat gtacctagtg
     2041 tctcaagagc tataaaggat attctatttt ctaatagcat ttatattaat tccctaaaac
     2101 taggtatagc gaattatact gctttagcta taaaaataaa accagaagta gataagatga
     2161 taggttctga agtaaatatc aacacaatag ttgtatctat aaagagacta gcagacgcat
     2221 tacaacaaaa tcaagagctt gaaagtatca cccaagaaga gaaagagaac ttggctggcg
     2281 ctagaatatc attaactgga agcatgcttg atgttgaatt tgataaagag atggagaata
     2341 ttgaaaaggt ctttgagtta tttgatagag aatccgatat cagatttaat atttttcaat
     2401 caaaaaatca catgaattta ttcatagaaa atattagtga aataaaaaag atctttagta
     2461 atcactccca aactattaac cccagcaaaa taaaggaagg agtttcaatg attacaattt
     2521 ctcttccttg ggaagaatcc gagttgagaa aaacatatca attgttgtcc atgatatcta
     2581 ctatcctcta tgataatcag gtttttttgc ataatgcatt ttttactcca gacgagatag
     2641 ttttaataat aaatgagatt gatgctgcaa aagtatatga attgctaagg gtcaaattcc
     2701 atagataata aaattctaaa agaagaaaaa tacgtcgagc ttattccttt tttaggaaaa
     2761 gataattttt tgataatttg gcctttttat cacatagaga tctttgcgta tactatgttt
     2821 gtccttttta tggcctttaa aattttgctg atcgtgtttt ggcgatattt ttatatttat
     2881 aattatttca taaaattttg tgtttatttg taatttttcc ataatataga tttaggtaat
     2941 ttttttgtta taaccaagaa aattattaac gagattggtg acattgaaac caaaacctta
     3001 tttcatctat cctcattatt attagtagga tattttactt ttgatttgaa ttgatttcat
     3061 ttatctcatc atttctttcc gtgttggttc tataaccaac tgtttctgtt gtttaatatt
     3121 gaactgttag cttcactgct acagagagat aaaatgatcc gttctgctgt tttaatgtta
     3181 acatatagaa atatagttgc tgtagtggac aaaattttta taatttggca aatcttattt
     3241 ccgttagtct acatttttgt cgcaggatat gcatactctt cattaatcac cgaaaatgaa
     3301 atagtaatag gtggaaaaac tattccttac aatgtatttt tagcgttagg aatgattgga
     3361 tttaatgtta tgaatagcag tactgtagct ggaagtataa tttggaatga taaacgaaat
     3421 ggaatgctcc atcagatttt ggtaatgcct tactccaaac ttcagtatat tgctagtaca
     3481 ctgttgacaa taatagttat gggtttggca agtgctgcaa ttattctcat tgctggtctt
     3541 ccaataattg caggaaatat aactattgat ataaccagta caatttatct tgtttttgcc
     3601 ttaataatgg gttcgatatt ctttggttca attgcaatta ttatttcaac aagactaaag
     3661 agcaatgaag gttttactgt aatcataaat agtgtttttt tattttttgc tttcgcaagt
     3721 tctacttttt atcctcctca tggactacct gaaccaatgc aatatgcttt ttatgttaat
     3781 ccattaacct atatcgctga catcataaga gagggaatgt tttcagcagt tgatacattt
     3841 acgaatatta aaattttagt attaggagta atatcaggga ttgcttttct aatagctact
     3901 ttctctatgt ataaaatgag aatttaattc tctcagtcaa gtgttattat tttttaatgc
     3961 ttttaaaaaa acttcttcaa tagatggatc atttaacgat atattttcta tttgaatatt
     4021 attttgaaag agaaagttta caatagctaa taagacgttt tgaggattat tggaagtaat
     4081 agaaaatgta ttttctgtat tttcgatatt ttctatatca attgaatttg cagataactt
     4141 ttgaaagtaa tcatggatat atttggaaat ggggttttgt aaaataatct ctatcttttt
     4201 tgttgaaccg tatttttgtt ttaaaatctg aggtgtatca aattctatta ttttgccttt
     4261 attgattata cagatttgat tacacaagta ttcaacttct tccataatat gtgttgtaaa
     4321 gaaaactgtt agaccacact ttacctgttt ttttatataa tttaatagat tccttctggc
     4381 tctggggtct aatcccacag taggctcatc taaaaataaa agatccatat cgtgtaaaaa
     4441 ttctcttgct atctgtacac gtcttctttg cccaatggat aaatcttcgt tacgtatttc
     4501 tttaatcgaa gttaattcaa atatgtctaa caattctctt actttgtctt tatttttatt
     4561 actagaattt ccccataaaa atccatataa ttccattgaa cgctcaactg tcaagttcat
     4621 ttcaaagctg ggattttggg taacaacgcc tatccgtctt ttaatttctt taaagttatt
     4681 tttttttata tctttctcta gtatttttat ataacttgcc gccgtaggct ctaaaatccc
     4741 tgtcagaatc tttattgtag tggtttttcc tgctccgtta ggacctaaaa acccaaatac
     4801 tgttccataa tcaatgtcaa aggaaatatc atctattaca tttcttttta aattagatga
     4861 atcatacgat tttacgaggt tttttactga aatacaatgc tgttgattat tattattatt
     4921 attattaaca gtattactca taagctgaat gacaacaaca ataacctata ttagaacacc
     4981 aataataatt cttattgatt acataccagc tattctattt gaaaaagatt ctgctatttt
     5041 tcttgaatct aaagatgatg attatattaa aatgtaataa aaaaaatcat tgcatgagta
     5101 aactataaaa aagtatggaa aacatatagg aaggataatt ataatattac tacttagagg
     5161 gtaataactg ctttgtcttt agattttaaa aaaatgatag aaagtgtgct tcaaaagaaa
     5221 ccagatatta gtgcagaaga tgtcagagaa ctaatagacg aaaaaaagag aaagatagga
     5281 gctggatatt tgacagatca gggtgctctt tttctagtgg cagcggacct ggggatatca
     5341 tttgaaaata accaaagtac ccagacctca ataaaagatc tatatattgg cgccaacgat
     5401 gtaaatttat ctgccagaat aatcaatatt tatcctgtta gaaaatatac cagaagggat
     5461 acaagcgaag aggttcaaaa cagaactcta acaatatatg acaaagattc ttcaatcaaa
     5521 ttaaggttat gggatcagca tgttagtttt ccagaagatt acgacgtcaa gccaggtgat
     5581 ttgataaaaa tttctcatgg ttatacaaaa tctggcttgg acaataaagc aattatcaat
     5641 cttggatcaa agggaataat agaaaaaagc agtagcacta ctaatgatga taatttggaa
     5701 attccttcac ttgatgctat ttcaaaaaca gtagatgaga taaaagaacc tgttgataat
     5761 attatagtaa caggggttct cagctcaaac ccgagagtat cccggtatgt caattttcgg
     5821 aatgaacaag gtaagtctct tcatatacct ttatcaaatg atgacggaac aaagactgta
     5881 cgttccatta tctggaacat agatgaagat aaaataccag gtatttttac acaaggatca
     5941 aaagtaaaat tgatcggtgt aaaagttaag cagggaaatc cacaatacag caacgaggaa
     6001 tttgaaatac acggagatga aggtacaatt attcaggttc aacaaataaa acgtgtgggc
     6061 gaagaaggtg aaggtattga tgctgaaggt gaaggagaag aagaatttga tggaagcaag
     6121 gtagtagtct taaaaattct ctcaacagga acagaatttg aaagttcagt aaatgagaat
     6181 tttttggcac ttgataaaga aaataaaaat tttatttctc tagttgttga tcctaaatta
     6241 atagaagaga aaatttatcc tgggataatt attgaaggga tacctaaccg agtattcgga
     6301 aatgtcatgt attttaccga tgagagttct tatataagaa tagttacaga atcttatgaa
     6361 agtgcatttc cacaggattc agaaatagaa acaaaaataa aaaatattca agtttcagag
     6421 aaaccttata ttgttgaagc gattgtttta caagcaccta atacctcaaa tattactact
     6481 aaaagtggtg aggatataac ggtaagtgag acacttattg gagatgatac tggagaaatt
     6541 agacttacag gttggagaga tcagagttcc aaaatttcga aattaaatgt tggagatagg
     6601 gtgaaaataa taggagcagt agccggaatg ggaagagaga acagaataga agtaacggta
     6661 aagcctttca cagaaataag agaattggag tagtagtaat agtaaatatt gagctagaga
     6721 aatgttaaaa taaatgatag ttcaccgaat ctctgaccta attgttgaag atttccaaat
     6781 ttaacatttt aaccttcttt atgataggca ccattttttc cttgttgtta attaccaacg
     6841 atttaaatct aaaagttaat tcattaaata ataatgaagc caaattcctc attgaaatat
     6901 tacagctaga aagtgctaat gctctttctt caaaaggagt tggtggatta gaaaaagtta
     6961 gcaatgaagc attacaacct cttaaagcag gagacaataa agatttaaaa aatactacct
     7021 tacaaactac aggatta
//
LOCUS       DASVEV010000314        15626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1729534, whole genome shotgun sequence.
ACCESSION   DASVEV010000314 DASVEV010000000
VERSION     DASVEV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15626
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729534"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VF222_13700"
     CDS             <1..1270
                     /locus_tag="VF222_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143526.1"
                     /translation="LKNGSRNAINSSTNIITKKSKAKEIFIRNAERDIKINWKRFMLS
                     NIENPLPSILDDLEIRADRLVHQLFAKEEEEENKQIIFRPEFTIRNIKIPLEGRLDLI
                     KIKLSNKERGKGDNCYNGIQYLNSYVTAEDLVNLEKESVEIVQIKTGNYRPRTAVWNL
                     QADAEALLLMQTLNLKEPPKYTWQFADKDGNRKKFDFAKVYKVIDKYIQIWKSEVSPQ
                     ITGFCPKCPLRDGCLNWAFVSNNALTEDERIKRNIEFRLSKRIRKEVSLDDRWKAYVV
                     LQSAEKRQREGSAITNLYIDTSSIDAEKQKITLLGDEYFGQFLDFSVGEHVTISDNTP
                     NLGSNPNAVIREIDIDRKSITLEFYRDDLYYLLYDNRDKSPFTIDRFNFSSGLISMKF
                     LDSFFRLSSYANDIVLKKRNVRLNEQNIIG"
     gene            1311..2630
                     /locus_tag="VF222_13705"
     CDS             1311..2630
                     /locus_tag="VF222_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX7143527.1"
                     /translation="MNIGRKFPSFDGIENLEQKTARYRINSEKTVPVTGPPGTGKSTV
                     ISHCCFDLLNDDYFPILVTAPTNVMIDSILSKIDSLLQREKIQLPTGFVIRYGNVANL
                     HRTYNHLIKYAFDNIVEDYYSSGYSNCTDNNNNNCYNNKITIAKDFFQSARIILSTDY
                     SAKDLGNIIKVGAVIVDEAGLVGLDRMGMLFSSLRDNNGKIIVIGDDKQLPPVSQDYV
                     TESLFGSILEHFQTTLLKREYRFNKDILDLINPYYEYKLEADSSVMNISTADIANKNY
                     DGINDNLYRVLKHDKNVVFVDTDGKCKEEKHFTNKGEVAIIKEIVDGCISMGIKDIIV
                     TTPYKQQERLCNFYLYNGIRIGTIDEFQGQEAEVTIISMVRSNNKTNYQEAIGFVNIP
                     RSCVAFSRSKRKTIVVGDRNTLMKNKFLSHSIDTITQKDGFILWRKY"
     gene            2686..2928
                     /locus_tag="VF222_13710"
     CDS             2686..2928
                     /locus_tag="VF222_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143528.1"
                     /translation="MKIKFGKSISIVHIKRYKNGALKTLNIQVQEIKKEEKDQDKDFI
                     IALIQLLQKNNSLLLQISFTTPVADFIRNNAIEQHK"
     gene            complement(3534..3794)
                     /locus_tag="VF222_13715"
     CDS             complement(3534..3794)
                     /locus_tag="VF222_13715"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX7143529.1"
                     /translation="MQSKQYNMTRDFIDYNPGDIIKKEARGVDEADLGEVQEISGEYI
                     IIQKGKVDKDKFYIPKNLLERFDGDIVYLRVTEEEVKQYKKE"
     gene            complement(4245..4424)
                     /locus_tag="VF222_13720"
     CDS             complement(4245..4424)
                     /locus_tag="VF222_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143530.1"
                     /translation="MGCFDSKDCTGGGDLSNIAATYIGIIIGAVIGAIISWWIYNRQQ
                     KTADEQEKILKQIED"
     gene            4642..4806
                     /locus_tag="VF222_13725"
     CDS             4642..4806
                     /locus_tag="VF222_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143531.1"
                     /translation="MMKSSRSASNGTDLKEDIKNILIEWHIGTEDMFDDEDLECMTDD
                     IMKVIKNQPK"
     gene            complement(5455..5784)
                     /locus_tag="VF222_13730"
     CDS             complement(5455..5784)
                     /locus_tag="VF222_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143532.1"
                     /translation="MNIDPDIRFVTIFDINDNIFLFSEHRQGVTNLLTKEESKHSLQI
                     ALGAWKMRRTLENKIGKGKFVLAEYEKIKRITMPLDKNHLLYITTEAGCDALSLVDKI
                     KKLLIIN"
     gene            6184..6360
                     /locus_tag="VF222_13735"
     CDS             6184..6360
                     /locus_tag="VF222_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143533.1"
                     /translation="MDTKSSIKKVLKWVFSGFSIFFICMLIFIVVHDVVTGQSSTTAE
                     YCTKYGFLASPDCW"
     gene            6818..6949
                     /locus_tag="VF222_13740"
     CDS             6818..6949
                     /locus_tag="VF222_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143534.1"
                     /translation="MTSSSETLICKECFRIFNTVEDLYEYQKSEEDQVERNKGFVKG"
     gene            7084..7524
                     /locus_tag="VF222_13745"
     CDS             7084..7524
                     /locus_tag="VF222_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7143535.1"
                     /translation="MLENWQIGLQLEFLIDIIFALAAGFVIGAERESRGKPAGISTNS
                     LVIGGAMLFTYLSAAVDPNSTSRIAAQIVSGIGFLGAGMILKGEIDKKITNLTTAASV
                     WFSAAIGMAIGFNFYIIALAAVIFAVVVPRIPHITKITSHREDE"
     gene            complement(8603..9208)
                     /locus_tag="VF222_13750"
     CDS             complement(8603..9208)
                     /locus_tag="VF222_13750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7143536.1"
                     /translation="MILNKNINVLGVASSMRKESTSTRALKLTLDIVKTNYGAEIKIL
                     DLRQTILPFLYYQEHDKKTEILIAEKMVKWADAFILSTPDYHGSMAGSMKNFLDYFWS
                     EFAGKLFGYICSSHEKGLTVMDQMRTAVRQCYGWSMPYGISINGEKDFRTNGNIRNET
                     LLQRIQMLARDIVIYGKLHRNQFLNDLSSQEINTFASHYRH"
     gene            complement(9411..9539)
                     /locus_tag="VF222_13755"
     CDS             complement(9411..9539)
                     /locus_tag="VF222_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143537.1"
                     /translation="MSNLQVAKKHWKACTVETEFFKKILDGEIETTHTAAKGDAIC"
     gene            10052..10417
                     /locus_tag="VF222_13760"
     CDS             10052..10417
                     /locus_tag="VF222_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7143538.1"
                     /translation="MKVSNKKFNSRSETKTLEEKCDILDIWDVLGRRWSLLILKNLSI
                     KKVVRFNELKRSLKGISSTVLSDRLSELEQEGLITKKIYPEIPLKVEYTLTTKTKDLE
                     LILQSLNKWCEKWESKISL"
     gene            complement(10690..10914)
                     /locus_tag="VF222_13765"
     CDS             complement(10690..10914)
                     /locus_tag="VF222_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143539.1"
                     /translation="MYDSHGYGQSKQKIRSGKAGGQSAVVSSNRKTIVTVTESDILEN
                     LMKKLKHINDKSEKKIGVISIQSVDALVHL"
     gene            11363..12211
                     /locus_tag="VF222_13770"
     CDS             11363..12211
                     /locus_tag="VF222_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX7143540.1"
                     /translation="MRLFYTRFDNWKNLIIEVDGKVHAKEHRKTLDRIRQRALENMGY
                     TVHRVKNEEIRDKPNDIADEINEIYTILSDTKDKKETSITELKKPLYIEPIPKVIQFN
                     LDSWARSLNEELKDERWSVDFFKKSLSQFSPELLKNQCAIEKLILLLHGLSLRKTQDG
                     NNLVFEYSLNFLKKGLNLLEEIFPENGNMAVIHLKNMFNETSPGFFKNLIFRGGPNIN
                     PGIVSIKSKDSLNCHIDSFNKNLSELGITVEYSDIIQECKATFQKFNEKEKIDYNWLI
                     ELMDTN"
     gene            12418..12795
                     /locus_tag="VF222_13775"
     CDS             12418..12795
                     /locus_tag="VF222_13775"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HEX7143541.1"
                     /translation="MNYNKLNPEEERVIIKKATEPPFTGEYDNFYEDGTFICRRCNMP
                     LFSSKSKFDSGCGWPSFDEYFPNAIKQVSDPDGIRTEIECANCHAHLGHEFLGEHLTD
                     KNTRECVNSLSIRFIPKDKELPK"
     gene            12796..13359
                     /gene="msrA"
                     /locus_tag="VF222_13780"
     CDS             12796..13359
                     /gene="msrA"
                     /locus_tag="VF222_13780"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7143542.1"
                     /translation="MNNNNTTTTEITTLANGCFWCTEAIFKKIKGVKYILPGYSGGTL
                     KNPSYDQVCTGKSGHAESIQIEFDPKVIPFEKILDIFWHTHDPTTLNRQGNDIGTQYR
                     SAIFYHDEKQKEIAEKSKRDLEIEDIYKDPIVTEITPFKNFYSAENYHKDYYEKHKDD
                     PYCNFVIDPKLRKLLQKYRNEVKEEYK"
     gene            complement(13631..14242)
                     /locus_tag="VF222_13785"
     CDS             complement(13631..14242)
                     /locus_tag="VF222_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143543.1"
                     /translation="MPQRVLQSSTSDISKQQMVSIQVPPNRRSEIEQFLKLFGYNSEK
                     TILQVHLIGFFRKKNLNQREIEQKSKSVRRYGGTSFIVVGRTYDPAIIENVIRPKSKD
                     PLESSPGYIGYLWKGKNLQNISPHELIPLDFTPVHLPYPDDWKKMFATKGFLIAMHVP
                     TSFVKDKNKLTRTLYRIAKNGLDIRKEWNFLNDINNLGYNSHI"
     gene            complement(15309..>15626)
                     /locus_tag="VF222_13790"
     CDS             complement(15309..>15626)
                     /locus_tag="VF222_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143544.1"
                     /translation="EALEMSGYDVSSFTDPSLAYQHIKENPNKYSLVITDDKMPDMNG
                     LLLGTKLLEINPKLNVIIMSDFGDLKCNYKFNLLKKRVSIFKLISAVNESISKSISHD
                     DKI"
BASE COUNT         5703 a         2082 c         2227 g         5614 t
ORIGIN      
        1 actaaaaaat ggtagtagga atgccatcaa ttcttctaca aatatcatca caaaaaaatc
       61 taaagctaaa gaaattttta ttagaaatgc ggaaagagat atcaaaatca attggaaacg
      121 ttttatgctt tcaaatatag aaaatccttt gccttctatc ctagatgatt tggaaatcag
      181 agctgataga ttagttcatc agttatttgc aaaagaagaa gaggaagaga acaagcaaat
      241 tatttttagg ccggaattta caattcgtaa tatcaaaatt ccactcgaag ggcgacttga
      301 tttaattaaa attaagcttt caaacaaaga aagaggaaaa ggggataact gctataatgg
      361 aattcagtat ttaaattcat atgtgactgc agaagattta gttaatttag aaaaagaatc
      421 agtagaaata gtacaaataa aaacaggcaa ttatcgacca agaactgctg tttggaatct
      481 tcaagcagat gcggaagctc ttttacttat gcaaacatta aatctaaaag aacctcctaa
      541 atatacatgg caatttgctg ataaagatgg taatagaaaa aaatttgatt ttgcaaaagt
      601 atacaaagta atagataaat atatacaaat ttggaagtca gaggtttctc cacaaattac
      661 tggcttttgt cctaaatgcc cattgcgaga tggatgctta aactgggcat ttgttagtaa
      721 taatgcatta acagaagatg aaagaataaa aagaaacata gaatttcgtc tttcgaaaag
      781 aataagaaaa gaggtttcac ttgatgatcg atggaaagca tatgtagttt tacagtcagc
      841 agagaaaagg caaagagaag gatctgccat tacaaatttg tatattgata cctcttcaat
      901 tgatgctgag aaacaaaaaa ttaccttact aggcgatgaa tattttggcc aattcttaga
      961 ttttagcgta ggtgaacatg tgactatttc tgataatact ccaaatttag gaagtaatcc
     1021 taatgcagtt ataagagaaa ttgatattga tagaaaatca attacacttg aattttacag
     1081 agatgatttg tattatcttt tgtatgataa cagagataaa tcacctttta ctattgacag
     1141 atttaatttt agtagtggtt taatatcaat gaaatttctt gatagtttct ttagactatc
     1201 ttcctatgct aatgatattg tattaaagaa aagaaatgtt aggctaaatg agcagaatat
     1261 tataggatga aaaaaaggta attattagat atgtgtggac cggtataaaa atgaatattg
     1321 gtcgaaagtt tccatccttt gatgggattg aaaatttgga gcaaaaaaca gcaagatata
     1381 gaataaattc agaaaaaaca gttcctgtca caggaccccc tggtactgga aaatcaacag
     1441 ttatctcaca ttgttgtttt gatcttttaa atgacgatta ttttccaatt ttagttactg
     1501 ctccaaccaa tgtgatgatt gatagcattt tatcaaaaat agattctctt ctacaacggg
     1561 aaaaaattca actacccact ggatttgtta tcagatatgg taacgttgca aatctacata
     1621 gaacatataa tcatcttatt aagtatgcct ttgacaatat tgtagaggat tattatagtt
     1681 caggctattc taactgtact gataataata ataataattg ctataataat aagattacaa
     1741 tagccaaaga tttttttcag agcgccagaa taatattaag tacagattat agtgctaaag
     1801 atcttggcaa tataattaaa gttggagcag ttatagtgga tgaggcagga ttagttggtc
     1861 ttgacagaat gggaatgtta ttttcctcat taagagataa taatgggaaa attatagtca
     1921 ttggggatga taaacaacta ccaccagtgt cccaagatta tgtaactgaa agcttatttg
     1981 gctctatcct tgaacatttt caaactacat tacttaaaag agaatataga tttaacaaag
     2041 atattttaga tttaattaat ccttattatg agtacaaact tgaagctgat tcttccgtta
     2101 tgaatatatc taccgcggat atcgccaata aaaattacga tggaatcaat gataacttat
     2161 atcgagtact taaacatgat aaaaatgttg tatttgttga tactgatgga aaatgtaaag
     2221 aagaaaagca ttttacaaac aaaggagaag ttgcaattat caaagaaatt gttgatggat
     2281 gtattagtat gggaattaag gatattattg ttacaacgcc atataaacaa caagaaagac
     2341 tatgtaattt ttatctatac aatggaataa gaattggaac aattgatgaa tttcaaggtc
     2401 aagaagcaga agttacaata atatcaatgg ttcgatctaa caataagacg aattatcaag
     2461 aagctatagg atttgttaat attccacgat cgtgtgttgc attttcaaga agtaagagaa
     2521 aaacaatagt agttggagat agaaatactc ttatgaaaaa taagttttta tctcattcta
     2581 tagacacaat aactcaaaaa gatgggttta ttttatggag aaaatattaa ttacaattta
     2641 gtatattatt tttctaatat tactgtaatt ctatatatca atacattgaa gataaaattt
     2701 ggcaaatcta tttctatagt acatatcaaa agatataaaa atggagcatt aaagacacta
     2761 aatatacaag tacaagaaat taaaaaagaa gaaaaagatc aagataaaga ttttattatt
     2821 gcgttaatac aattattgca aaagaataat tcattattat tacaaatatc atttactacc
     2881 ccagtagcgg atttcataag aaataatgcc attgaacagc acaaatagat catagaactc
     2941 aaccaaaaat ataatgactt ggttaaccaa aacgatagta agaaagaaat aaaggaagag
     3001 gaacaaaggg ttttagaaga atataattgc cggaaaataa ctttctatgt taaaataatt
     3061 tttatgatac ttcaaatatc atttgaatca aaattgtggt ggatgtttat atacaagata
     3121 aagaattagc tattcacgat aaaatttgtt tattatttca taataatgag cataatttag
     3181 acgggtttga tattgactaa aataggtttg aaactaggac tcacttagtg gtcttagata
     3241 atagcagaat agtttgatat tgactaaaat aggtttgaaa ccgtctatct ctcattcagt
     3301 caaacattaa caatcattaa atttttatct ttgagagtag aacccacatt atttttttat
     3361 aacataattt taattatatc tttatcttaa aaccttgata tttgctgagg agatttattg
     3421 agtttttcag atcggaagtg aaagattata tttatagaaa taaacaaaaa ttttattaaa
     3481 gagatactat acaaaagatt cttcttaaac taaaaaatta aatttgtaag aagttattct
     3541 tttttgtatt gttttacttc ttcttctgtt actcttaaat agacaatatc gccatcaaaa
     3601 cgctctagca aatttttagg gatatagaat ttgtctttgt ctaccttacc tttttgaata
     3661 attatgtact ctcctgaaat ttcttgaact tcaccaaggt cagcttcatc tacacctctt
     3721 gcttcttttt taataatatc tcctggattg tagtctataa aatcccttgt catattatat
     3781 tgtttagatt gcatttatat agttcttttt gagattgata aagatatctg aaatagttac
     3841 aaagatgtag taatctatga cctttaagtt ctattataaa tgaaataatt acttggtttc
     3901 ctataataat accaatacta attgcaagac cattcatcat atttctaatc ataaatgact
     3961 ttgaacatag gttatattat tttgacttgt tattttcctc ttttgttttt ttggttttca
     4021 ctcagaatag aattccatac caaacatgta ttaacaattt tttccggatt tgtctttcaa
     4081 ttttctacac atttttcaaa ttttttggct ctaatctctc tgtaacacct cgaagactat
     4141 tatatacata aaccaatatc ttttttttag agatacatta ggtaaaatac ttaatattaa
     4201 ttttcattct tgtttattct tattgagtct atttaccatt ctatctaatc ttctatctgc
     4261 tttagtattt tctcttgttc atctgcagtt ttctgttgtc tattgtatat ccaccaacta
     4321 attatggcac cgattaccgc tcctattata atacctatat atgtagccgc aatattggac
     4381 aaatctcctc ctccagtaca atcttttgag tcgaaacatc ccaacgttat gaatatatcc
     4441 atcaacatac taatagaatt tctacatact tcttttttat atttactgat gaggaatagt
     4501 tgtgaggatt aaattatttt ttatttttag ttctttcaat taagtaataa ttaatatacc
     4561 ataacaataa gatgtacgat aattaaatga gaaataatag aaaatgagat attgattaaa
     4621 tttaattaat tattttataa catgatgaaa tcttcaaggt ctgctagtaa cggtacagat
     4681 cttaaagaag acatcaaaaa tatattgatt gaatggcata ttggtacaga agatatgttt
     4741 gatgatgagg atctagaatg tatgactgat gatattatga aagtaattaa aaatcagccc
     4801 aaataaaatt taattctctt ctaaattatt tctcagttta attattattt gttaatcata
     4861 tttctttagt atttctttgt tcgcaaataa cctaaatttc taaaatctta tcacaatctt
     4921 atttatgctc tatatatcaa cttatcccga tctttagcat ctgcatcatg aattagttta
     4981 tctattttat acacgtacac ctatctcttt atccatgaaa tccgtattcg cttgttctcc
     5041 tctactgtta aaccttttta aattctctaa aatatttgca aaaacgttat tcctatcgaa
     5101 tctttaaaac atatattaaa ttcaagaata catagattag aatttgaatt aaaagtagca
     5161 agagaataga ggaaaccaat aaaaatgtat atcaacttga aatattggaa tgttatagaa
     5221 ttgtaataaa tagatatata cttgaaagca gaagaaaata agatagtcta actccatttt
     5281 gctagtattt aactatatct aaacaaaaca ggaattgttc tagaaggata atttctgtgc
     5341 aatataattt aaatttatct agtaataatt ttgctttcaa atagaatatt atctggataa
     5401 tatctatagt gattaatgct gatctaatac ttaatttaac taattattct ctagtcaatt
     5461 tatgatcaat aattttttaa ttttatcaac taggctgaga gcatcacatc ccgcctcagt
     5521 agttatgtaa agtaaatgat tcttatctaa aggcatagta atgcgtttta tcttttcata
     5581 ttcagcaaga acaaactttc cttttccgat cttgttttcg agtgtacgtc gcattttcca
     5641 tgcaccgagt gcaatctgaa gagaatgttt gctttcttct tttgttagga gatttgttac
     5701 cccctgccta tgttcagaaa aaagaaatat gttatcatta atatcaaata ttgttacaaa
     5761 acggatatct ggatcaatat tcataatttg ctttcctata tttttataat ccattattaa
     5821 atagttagtt atgttagttt gtataaaaag tttgtagtaa aaagtttgct tatttacctt
     5881 agaaaagatg ctgactttct atagacatgg ttgatcgata tattgttgtg tagatgaata
     5941 aatctgattt ggcagaactc tagaaaacta ctaaatcatc tccaaagcat atagatttgc
     6001 tagaatatta ttttagtaaa gaaattcact aatacaacta ctaattaata ataatagata
     6061 tccacatttt aagagtttta ttactattga ttgaactaaa attctatgca ttctcatttc
     6121 ggagaatgtt ttataataat tatatagaac tagatacaat ctcagttaat gatgttatcg
     6181 gttatggata caaaaagttc tattaaaaag gtattaaaat gggtatttag cggtttcagt
     6241 atatttttta tttgtatgtt gattttcatt gtagtccatg atgtagtgac cggtcaatct
     6301 tcgacaacag cagaatattg tacaaagtat ggctttttag catcacctga ttgttggtaa
     6361 aataaatggg tataaaagga aagtaaagta accttcaaaa acttatacca attaagttaa
     6421 cagaacccaa ttttttattg cctaatataa ctccaaaaat tcctttttat tgcctaatat
     6481 aactccaaaa attccatctc caggcctagt attttatcaa tttggctcat ctaaatgggc
     6541 tagggcaatt ttaaagatat tgatgaactt ggttcaccta ttcataaata ttctataatg
     6601 taacaaataa tactcaaaac tatttaggca tacaaaggta gtcatagcca gtataactca
     6661 ctcactccat gatggtcttc tgctggctat ggctattaat taactcaaag ataaaattat
     6721 ctcaaatttt aattcatcat atcgactttg acttcaacac ttttaataat aaaataattt
     6781 tagttaaata ttttgttaaa gtatgtaata ttctataatg acatctagta gtgaaaccct
     6841 gatttgtaaa gaatgtttta gaattttcaa tacagttgag gatctgtatg aatatcaaaa
     6901 atcagaagaa gatcaagtag aaagaaataa gggatttgtt aagggttaat tactatatat
     6961 tttagaatcc actcaatcaa tcaattttta aatatattta caaattcata tgttatgttg
     7021 ttaggattta ttttaagtta ataataatag gatttataga taatactgta tatcccaatt
     7081 aaaatgttag agaattggca gataggtcta caacttgagt ttctaataga catcattttt
     7141 gcccttgctg ctggattcgt tataggagca gaaagagagt ccagaggtaa acccgctggt
     7201 attagtacaa attctttggt aatcggtggt gctatgttgt ttacatatct ctctgctgct
     7261 gttgatccta attcaacttc acgcatagca gcgcagatag taagtggaat agggttttta
     7321 ggtgctggaa tgattcttaa gggagagata gataaaaaaa ttacaaactt aactactgca
     7381 gcaagtgttt ggttttctgc agctatagga atggctatag gatttaattt ttatatcata
     7441 gcgttagcag cagtcatatt tgctgtagta gtgcctcgta tacctcatat tacaaaaata
     7501 acaagtcata gagaagatga ataagaataa acaaacaaaa aaagatgatg ttctataaag
     7561 atgaaggtat aactgaaatg taaatgatat gtgatagtta taagtaactt aatctatgat
     7621 tatttctgat tactttcttt ttcgatagtt ggatacattt cccgatagtc ttctaaacca
     7681 aagtgtaggt cctttcttca caaatgataa tcggttcgta aataaattct atatgtgata
     7741 caggaagatt ctgttataaa tggaggatat ataattgaaa ttggttatga gcaaagaaaa
     7801 cttcctcata cactggaaaa gatgttgaga taaatgaata atttttgtat ctaaacatct
     7861 atttttaaca ataatatatc aataactata attaggataa tattttcatc aatattaaat
     7921 aatatattta aaatataatt ttacaaaaac aattctttca aggaaacagt cttaatcaca
     7981 ccatagccta tagagtaaat cctatttggc tatggtaatt cactaaacta ttatgcccaa
     8041 ttattacttt attttttata aagataaata aagtttagtt tgttaagata aataagattt
     8101 ctctaattac atggtatcaa tctttattat ttcatttatt agtaaatgca aaatattaac
     8161 tatttaaatt taaatgtgta gtttttgtcc agttatttgt aggaactaag gagaaagata
     8221 gtcaacggtt tttggcagaa ttttgaaata caaaataaaa aaaaatgcga attagtaaat
     8281 tataaatgat acttattatc aagaaaatag ttataaaatt cctacgattt cagtaataag
     8341 attttaccaa tttacctatt ccaaagattg ctagctaaat aaaagttaat acaagtctaa
     8401 tgtttttcat ttgaggagcc taaatatatc tacctatgtc attgtttatt agaatataat
     8461 aaggaattta tgtaaacccc tagctggaac aattaaaata gttatggatg gtgatccaat
     8521 aattaatagt tctataggaa atacagcaat aatcacaaaa aaatctatgt aaaactgaat
     8581 ctaattacaa atcagttata aatcaatgtc tatagtgaga ggcaaatgta tttatttctt
     8641 gggaacttaa atcattaaga aattgatttc gatgtaactt accgtatatt actatatctc
     8701 gagctaacat ttgtatcctt tgtaataatg tttcgtttct aatatttcca tttgttctaa
     8761 agtctttttc tccatttatt gagatgccat aaggcatact ccatccataa cattgtctca
     8821 ctgctgtgcg catctgatcc attactgtca atcctttttc atgagaagaa caaatatatc
     8881 caaaaagttt tcccgcgaat tcagaccaaa agtaatctag aaaatttttc atagaaccag
     8941 ccatagaacc atgataatcg ggcgtggaaa gaataaaagc atcagcccac tttaccattt
     9001 tttcggctat taatatttcg gtttttttat catgttcttg ataatacaaa aatggtagta
     9061 ttgtttgtct taaatccaaa attttaattt ctgcaccata atttgtcttt actatatcaa
     9121 gtgttaattt taatgctcta gtactagtgg attcttttcg catactagaa gcaactccta
     9181 gaacattaat atttttattt agtatcacat taatttatta tcttctatat tttataagtc
     9241 taaaggttta caaattataa aaatataata taataaaaat ttattcttca aaaaatattt
     9301 tactaatagt ggttactatg tttacatttg tatgttcatg ctattacaat ttaataataa
     9361 gttatttttt atatttacaa aaatccttgt ttatttggtt ttattataaa tcaacaaata
     9421 gcatctcctt tcgcggcagt atgggtagtt tctatttctc catcaagaat ttttttaaaa
     9481 aattctgtct ctactgtaca tgctttccaa tgtttttttg ctacctgcaa attggacaat
     9541 taaattcaag taattcatat gttccatttc tttaattttt tattcatttt ttttgattct
     9601 gtcatatacc cctcttcatc acggatcttt gccaattctt ttatcttatc gtcaaattct
     9661 aaactattaa gacttggtta tattgcaaaa agagctgggc ttgtctttca catagtaaat
     9721 ccatgacacc ttcttttccc agtttctttc aatatgatcg aaagcagaag atgctatagt
     9781 actatacgat cttctactaa ccactttagt tcctcaaata catactttaa cagaaatgtt
     9841 atcaattcct tttcacctaa gactttttat cggtattaca ataacagttc tgttaaggac
     9901 ttgtttaata atgcctcttt tgactaaatt attaagacct tggttatgca aaaaattgaa
     9961 atatataatt attgctttta gatcggataa gtgtaattag tcctattgtt ttaatattta
    10021 ttactataat atcatttaat ttagtatgtt ggtgaaagta tctaataaaa aatttaattc
    10081 tagatctgaa actaaaactt tggaagaaaa atgtgatata ttagatattt gggatgttct
    10141 aggtagaaga tggtcattac taatattaaa aaatttaagt attaaaaagg tagttagatt
    10201 taatgaatta aaaagaagcc ttaaaggaat tagcagtaca gttttatctg atagattatc
    10261 agaacttgaa caagaaggtt taattacaaa aaaaatatat ccagaaattc cactaaaagt
    10321 tgaatacaca ttaacaacca aaacaaaaga tcttgaacta attttacaga gtcttaataa
    10381 atggtgtgaa aaatgggaat ctaaaatatc tttataatga aacctcatga tcattattta
    10441 taaaatataa taaattggga tgagatgcaa tttagacttt tgtacctata atagtataaa
    10501 gtaatgctat atgtggaatt taaggaataa atcttttgag gaataatgct tagttgaagg
    10561 acgtgacggg atttgaatct attcttgtat ttaagagttt aaaataatct gaattctata
    10621 tacttttaag tcaaattacg attttttcaa aaatattttc agcgagaaaa aactggagta
    10681 agaatcatgt tatagatgga ctaaagcatc aactgattga atggagatta caccaatctt
    10741 tttttcactc ttgtcattta tatgcttgag ctttttcatt aaattttcta aaatatctga
    10801 ctctgttact gtaactatag ttttacgatt tgaagatact actgcacttt gtccccctgc
    10861 tttcccagat cttatttttt gtttggactg tccataacca tgagagtcat acaattttgc
    10921 ctctaatttt aaatcctcta cttttgatat tacttcatca acttgttctg gactaacaaa
    10981 aatttctact ttttttaagc ctgcagaaat tttattttta tcactttttt agattctacg
    11041 atctcaaagg gtaaaactaa attcttttgt caatataaaa atacaaacaa tcgaaagtaa
    11101 taaagtattt gttaatagaa tttatcagct gaaagttcat aaagttaagt ctagtcaatc
    11161 taaaataaca agttaatttg taaatgcaaa aagataaata aaatctgtag tactcttttt
    11221 aaagagcatt atacatcatg aaataattaa tggacaatta ttacaagccg ccgaagacta
    11281 caacatctga attgaagttg agaaatatat tgaaatctaa aaaaattccc ttttatcaca
    11341 gtaaaattat ttggtataca agttgcgact attttacacc agatttgata attggaaaaa
    11401 tcttattatc gaagtagacg ggaaggttca tgctaaagag catagaaaaa cattagatag
    11461 gatacggcag agagcattag aaaatatggg ttatactgta catagagtaa aaaatgaaga
    11521 aattcgcgat aaaccaaatg atatagcaga tgaaattaac gaaatttaca ctatattatc
    11581 ggatactaaa gataaaaagg agactagtat aacagaatta aaaaaaccac tatatattga
    11641 acctattcca aaagttatac agttcaattt agactcttgg gctaggtcat taaatgaaga
    11701 attaaaagat gaaagatggt cagttgattt ttttaaaaaa tcattatcac aattttctcc
    11761 tgaattactt aaaaaccaat gtgcgattga aaaattaatt ttattgttac atgggcttag
    11821 tcttcgaaaa acacaagatg gtaataattt ggtttttgaa tattcattaa atttcttaaa
    11881 aaaaggttta aatttattgg aagagatatt tccagaaaat ggtaacatgg ctgttataca
    11941 tctaaaaaat atgttcaatg aaacttcgcc aggttttttc aaaaatttaa tctttagagg
    12001 tggaccaaat ataaacccag gaatagtttc aatcaaaagt aaagattctt tgaattgcca
    12061 tattgatagt ttcaataaaa atctgtctga attaggaata actgtagaat attcagacat
    12121 tatacaagaa tgtaaagcaa cttttcaaaa gtttaatgag aaggaaaaaa tagattataa
    12181 ttggcttata gaattgatgg atactaatta aattctacat tctttattta acaatggata
    12241 atttgttgca ttattactat ttcctagcta tgacgggcca tgcaatatat caacgtatat
    12301 gttctgcatt caatttttat aaataagcaa ttagattatt caattgataa ggactatatt
    12361 acaaaatatt atacgaaaat cagaagattc aaaaatctgt tgatgaaagt taatagtatg
    12421 aattacaata aactaaaccc agaagaagaa cgagtcatta taaaaaaagc aacggaacct
    12481 ccttttactg gagagtatga caatttttac gaagatggaa catttatctg tcgaaggtgt
    12541 aatatgcctc tattttcatc aaaaagcaaa tttgattctg gatgtggatg gccaagcttt
    12601 gatgaatatt ttcccaatgc aataaagcag gtttccgacc ctgatggaat aaggaccgaa
    12661 atagaatgtg caaattgcca tgcgcattta ggacatgaat ttcttggaga gcacttgaca
    12721 gacaaaaata cacgagaatg tgtcaattca ttatctatcc gttttatccc aaaggacaag
    12781 gagttaccaa aataaatgaa taataataat actactacta ctgaaataac aacacttgca
    12841 aatggatgtt tctggtgtac agaagcaatc ttcaaaaaaa ttaaaggtgt caaatatatt
    12901 cttcctggat acagtggagg aacattaaaa aatccttctt atgaccaagt atgcactgga
    12961 aaatctggtc atgcagaatc aattcaaata gaatttgatc ccaaagttat accttttgaa
    13021 aaaattcttg atattttctg gcatacccac gatccgacaa cgctcaatag acaaggtaat
    13081 gatattggca ctcaataccg ttctgcaatc ttttatcatg atgaaaaaca aaaggagatt
    13141 gcagagaaat caaaaagaga cctagaaata gaagatattt acaaagatcc aattgtgaca
    13201 gaaattactc cttttaagaa tttttattct gcggagaatt atcataaaga ttactatgaa
    13261 aaacataaag atgatccata ttgcaatttt gttattgatc ctaaactacg gaaacttctt
    13321 cagaaatata ggaatgaagt aaaagaagaa tataagtagc ttttatcaat gacttttatt
    13381 gtatgaatgc ctgtgaaata atactatgga ttaaatttaa ttcaattcaa ttcaataaga
    13441 tggttattcc tgtattaata cgtgacttaa tgagctttga attctcatta tcaggcatag
    13501 tctataatat aatatcaata aaaaatgtaa attaaagaag aatctctatt tatgctatat
    13561 ggactaggag atctttgaga gtatgaaatg taattgagat ctgatgcaga atttaaagat
    13621 taacttttta ttagatgtga ctattataac ctaaattatt gatatcatta aggaaattcc
    13681 attcttttct tatatcaagc ccattttttg caatacgata aagtgttctt gtaagcttat
    13741 ttttatcttt tacaaatgac gtaggaacgt gcattgctat taagaatcct ttggttgcaa
    13801 acatcttttt ccaatcatca ggatatggta aatgtacagg agtaaaatct agcggaatta
    13861 attcatgagg tgatatattt tgtaggtttt tacccttcca gagatatcct atatatccag
    13921 gacttgattc taaaggatct ttactttttg gtctaattac gttttctata attgcaggat
    13981 catatgttct tccaactact ataaaggagg ttcctccata tctccttact gattttgatt
    14041 tttgttctat ttctctttga tttaaatttt tttttctaaa aaagccaatt aaatgtactt
    14101 gaagtatagt tttctcagaa ttatatccaa acaattttaa aaattgttcg atctcgcttc
    14161 ttctattagg tggaacctga atagatacca tttgttgttt tgatatatct gatgttgatg
    14221 attgtaacac tctttgtggc atattccttt ttcgatacct tatttgatta tttttattat
    14281 catgaagatt atgcatacta ttattatttt gttttcttaa ttaataactg atatacaatt
    14341 taaaaataat atttcttttt ttatgaaatg aattccaatg atatttgtac tcgaatactt
    14401 tagattgcat ggattttgga aatgacattg gaaataaaaa ggtcttcaat tcaattaatg
    14461 taatgacatt tagttgttag attaaaaaga tttgttagta attgatcttc acatatatta
    14521 tattcttagt ctatccagtt atcatactat tcttaacaat cttctttaga atttataatt
    14581 gatgattttt acattctctt caaatctatt aatatcatta ttcgcggttc tgtaaagtct
    14641 tagtcggttt gaaagagctg tcaaaaaccg tcggatatta ttcatgacaa atcaaggaga
    14701 taaaacatga cggagaatga agattttaat tattcgatag atatatacat ttactatttt
    14761 ttgtatagca aacctgaaat ctttgtttaa cgtatatatt attattatga tcttaattca
    14821 tggctatgat aactttattt tttatattgc gatgttctta atgataattg taatacttgc
    14881 aatattattg acgtttgtct attctcccta ctattatcac aatattaaaa atattataat
    14941 tttaccaatg atagagaact ttaacttatt atgatactag taaaataata taatgatttt
    15001 tagtatgata gatagatttg attcttcatt acatcagtca tatttataat ttataattta
    15061 taatttataa tttaacaata atcattttca ttaacctttt tttgattaat aaatggttgt
    15121 gcccgttact attaaattga atcaacctat tttatacttt gtagtacatc tattgataca
    15181 gaaggaggga tgtaagtctc gaatactgta gagaggttga gataatggat aaaatcgttg
    15241 tattacttct attttttaat cataatataa taaatataat aaataattat aatgaataaa
    15301 aattctattt agattttatc atcatgtgaa atagattttg atatactttc attcactgca
    15361 ctaattaatt taaatattga tacacgtttt ttaagaagat taaatttata attacatttc
    15421 aagtcaccga aatcactcat tatgattaca tttagttttg gatttatttc taagagttta
    15481 gtaccaagta ataagccatt catgtcaggc attttgtcat cagtaattac taatgaatat
    15541 ttattcgggt tttccttgat gtgttgatag gccaatgaag gatcagtaaa actagaaaca
    15601 tcataaccgc tcatttctaa agcctc
//
LOCUS       DASVEV010000315         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_175641, whole genome shotgun sequence.
ACCESSION   DASVEV010000315 DASVEV010000000
VERSION     DASVEV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175641"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VF222_13795"
     CDS             complement(<1..887)
                     /locus_tag="VF222_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7143545.1"
                     /translation="MPKKIAFGKDAAKEFEYPKKSLIITTTTPEIYNKWIEYMGITDY
                     ELYDKATPDPAIETVEQIKKAFEGRDISAYIGLGGGSSMDLCKYLSKLTGIPKILIPT
                     TFGTGAEMTTYAVISFEHKKKLLQDEAFLADAAIVDPYFLPGTPFNIMRNSACDAAAQ
                     ASEGYDSKLSNPFTKLFCKEAFDILEDAIINDKPDLLPYGAMLSGIGFGNSSTTLGHA
                     LSYVFSNEGVPHGYSLSSCTTVAHKFNESIFYERFKKICQKLKFEPLKLKQPLEEAAD
                     VIMPDRGHLDNNPREVTRD"
     gene            2387..>2595
                     /locus_tag="VF222_13800"
     CDS             2387..>2595
                     /locus_tag="VF222_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX7143546.1"
                     /translation="MRTFIAVDCNNKEKIGNIQQMILNSLPSLDGTIKAIDPENLHFT
                     LFFFGEVDDKKVERIKNKMNEITFQG"
BASE COUNT          972 a          366 c          372 g          885 t
ORIGIN      
        1 tcatctctgg ttacttctct aggattgttg tctaaatgtc ctctgtcagg cattattaca
       61 tctgctgctt cctctaatgg ctgtttcaat ttcagtggtt caaacttcaa tttttgacat
      121 atcttcttaa acctttcata aaaaattgat tcattgaatt tatgtgctac ggttgtacaa
      181 gatgataatg agtagccgtg aggaacacct tcattggaaa atacataaga aagagcatgt
      241 cctaaagtgg tagaggaatt tccaaatcca attcctgaca acattgcacc atagggaagc
      301 aaatcaggtt tatcgtttat gatagcatct tccaaaatat caaaagcttc tttacaaaat
      361 aatttagtaa agggattact aagtttacta tcataccctt ctgatgcttg agcagctgca
      421 tcacaggcag aattcctcat tatgttaaat ggcgtacctg gcaaaaaata tggatcaaca
      481 atagctgcat cagccaaaaa agcttcatcc tgtaacaatt ttttcttatg ctcaaaactg
      541 ataacggcat atgttgtcat ttctgctcct gttccaaagg ttgttgggat aagtatttta
      601 ggaatacccg tcaatttact tagatattta cataaatcca ttgaactacc tccacctaaa
      661 ccgatatatg cagaaatatc tcttccttca aatgctttct taatctgttc tactgtttct
      721 atagccgggt caggagttgc tttatcataa agttcataat ctgtgattcc catatactca
      781 atccatttgt tataaatttc tggcgttgtt gtagtaatta ttaaagattt ttttggatat
      841 tcaaattctt ttgcagcatc ctttccaaaa gctatcttct taggcatcat tactttccac
      901 ataatactca atgagaagtg ataaattatt aaatttaaat ttatagtatc aacaaaaatt
      961 atacagtgtg agatactcaa taaattaagg taatggcaag tttcatgatt aacattcttg
     1021 ttttatatta attatccacg tcttgttagt tttactaata actagggttt attgtacaat
     1081 atgctagatt cttagtctta cactaattct tagaaattcc tattaaaaac aaaaaaaatt
     1141 ttctattttc tctacttatc tgaaagaatt acacaactac ataaggcatt ttcatattta
     1201 aaatacagag agaaaagagt attaaatcca aattatgggt tcgttagaca taggaaggca
     1261 aaagattcaa aaatttttaa gagttaagtg agaagagact tgaatatcgt agaaacataa
     1321 aaaaaagtga atcggataga cttcctttat attatcatta atttattata ttatgacact
     1381 ttagaagttt tagtaattat aataaggtaa gattagtcac atttgatcaa agtggtagta
     1441 aagatgataa aactgaaaag aagaaagaga atggtacatt catgtaaata atggcacaaa
     1501 atattctgaa aaatagacat aggagaattg atattcattc gatagacgaa attgataaat
     1561 tatttttaaa agagttctga cctaaaaaga gaacaaataa taacataatt taaagaataa
     1621 ccaaagactg aagagattag tttctcatta aacgaagttg tttaatagaa tataaatatt
     1681 ggatcaagtt atggaaattt gtaaatttta ataaattact agcaagttaa atatactttt
     1741 gcattgataa attgttaact ctgttaccag ttatagattt agacttagta atgaaatagt
     1801 actacatcag attgtaagac atagaaacaa tagttattga caatacagga aaattactct
     1861 tataatccgt caagaaaaca gagtatattt ttatataaga aaataaatta attattatta
     1921 ccatctaatg ataacaaagc ataatattat tacttaaaat aacattgcat aactaatcag
     1981 aaagaatggg aaatagtcaa attaattaaa ttaaagaatc acaaataatt gactaactta
     2041 tgtgatcccc tatgtttcta ctggaaaaat attctaatat tatattatga tcttttttat
     2101 catgaagaat atcttaatat tctaacattg taccaaatag gctgcacgac tccgcttttt
     2161 atcagttaaa gtactgattt gattcatttt atactataat agaaatattt cctctgcaat
     2221 ataatattct ctattagaca tgccaataga tacaaaattt acggaattat tatagtgggc
     2281 ttttcactca tttataatag caaagaagaa aatggtagcg ataatagcaa attagaataa
     2341 tattttaaag atatgaatat atgtttttga ttgatcagtt tttaatatga gaacgtttat
     2401 tgctgtagac tgtaacaata aagaaaaaat aggaaacata cagcaaatga tactaaattc
     2461 tcttcctagt cttgatggaa ccatcaaggc tattgaccca gaaaatctgc attttaccct
     2521 gtttttcttt ggagaggttg acgataaaaa agtagaaaga attaaaaata aaatgaatga
     2581 gataacattc caagg
//
LOCUS       DASVEV010000316         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_759468, whole genome shotgun sequence.
ACCESSION   DASVEV010000316 DASVEV010000000
VERSION     DASVEV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759468"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF222_13805"
     CDS             <1..323
                     /locus_tag="VF222_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143547.1"
                     /translation="ETGHHLTETETGHHLTEINHLTEISHHLTGTETGHHLTGTEISH
                     HLTGTETGHHLTETETGHHLTEINHLTQKRKRASISNFHQIKKVRKKENNNKNNNISI
                     LNLC"
     gene            491..1357
                     /locus_tag="VF222_13810"
     CDS             491..1357
                     /locus_tag="VF222_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7143548.1"
                     /translation="MKKIVLDNSELEYSIFQSFNKESVININGIRNNIVLIHGGIIAD
                     ANIPLVTFSDTLTKSYNILHYHRCGYGKSINKKNDHTDILQHVEDCRGILDFLDIEKA
                     HIVGHSIGGTIALQLASTYPDYIKSLILLEPAITGYNESTNEQVIQDFQPIIQMYDKG
                     QKNEAIDIFMKTAIGTNYKEIIANVLPSNSFELAVADAKTFFHEEIPAMRSWRFTKTQ
                     TKDLVDVPVLHIRGIQKTRKISKEREELLNYWLPQTVTTSIQNAPHMLQITHTKEVVQ
                     LIELFFQQINTL"
     gene            1851..>2074
                     /locus_tag="VF222_13815"
     CDS             1851..>2074
                     /locus_tag="VF222_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143549.1"
                     /translation="MDIKPLFVLYSVVAITIVLSFSSIDAQINGNGTSGNITSSTKSM
                     GETMANQSTAVVDNATNSLGETMANKSSAVV"
BASE COUNT          832 a          277 c          309 g          656 t
ORIGIN      
        1 cagagacagg ccaccattta acagagacag agacaggcca ccatttaaca gagataaacc
       61 atttaacaga gataagccac catttaacag ggacagagac aggccaccat ttaacaggga
      121 cagagataag ccaccattta acagggacag agacaggcca ccatttaaca gagacagaga
      181 caggccacca tttaacagag ataaaccatt taacacagaa aagaaagagg gcttctataa
      241 gcaattttca tcaaataaaa aaggtaagaa aaaaagagaa taacaacaaa aataataata
      301 tttctattct caatttatgt tagcttacct gcaatttgtt ttgaaataaa gttacaaatt
      361 actgcaatat ttcaaaataa atatcaataa ttaaagtcgt cataaaacaa tggtattata
      421 attttttgta atgttattaa tccaattcaa atatttattt cacactattg ttaatatcta
      481 ctaaacaata atgaaaaaaa tagtgcttga taattcagaa ttagaataca gtatcttcca
      541 atcatttaat aaggaatctg taattaatat caatgggatc agaaataata ttgtccttat
      601 tcacggaggt attatagctg atgcaaatat tccattggta acattttctg atactttaac
      661 taaaagttat aatatattgc attaccatcg atgcggatat ggtaaaagca ttaacaaaaa
      721 aaatgatcat acagatatac tacagcatgt ggaagattgc agaggaatat tggattttct
      781 tgatattgag aaagctcata tagtagggca ttctatagga ggaaccatag ctttacaatt
      841 ggcgtctact tatccagatt atataaaatc tttaattctt cttgaacctg caattacggg
      901 atataatgaa tctacgaacg agcaagtaat acaggatttt caaccaataa ttcaaatgta
      961 tgataaagga caaaaaaatg aagctataga tatttttatg aaaacagcaa taggaactaa
     1021 ttacaaagag ataatagcaa atgttttgcc ttcaaattca tttgaattag cagttgcaga
     1081 tgcgaaaaca ttttttcatg aagaaattcc agctatgaga tcatggagat ttacaaaaac
     1141 acaaactaaa gatttagttg atgtgccagt tttacatata agaggaatac aaaagaccag
     1201 aaaaataagt aaagagagag aagaactact caattactgg ctgcctcaaa ctgttactac
     1261 atcaatacaa aatgcacccc atatgcttca gataactcat accaaagaag tcgttcagtt
     1321 aattgaatta ttttttcaac aaattaatac attgtaaaat aatcgtatta aattatatta
     1381 agaaaatggt taacaaattt taatagtcat tataaaatat agattttaat gttttatcct
     1441 tattaaatta tactttataa atcagtaatt tttgtgagga tttatagaat aaactgtcta
     1501 ttttattaac ctatttagtt tttatactat gtatgggata taataataaa ttaagtaaag
     1561 acagcatagt gatttattat taaaagaagt tacatgtcta tagttagtat acatataaaa
     1621 gtttatattt gagctaccta aaaaaaatga ttttattata tagaataatt gtgatatagt
     1681 taagatatgc ctcgttaaat aagtaataat atccattaaa ttgtaatagt tataaaccat
     1741 gtagataaat atcaaagagt tagttagtta tttagccctt ttatattaga acttaattta
     1801 aatcttttaa aattaaaagg ttaatatatg tagtttatgt taaaattgta atggatataa
     1861 agcctctttt tgttttatac agcgtagtag caattactat agtcttgagt ttttcttcaa
     1921 tagacgcaca gattaacggg aacggcacaa gtggaaatat aacgagttca actaaatcta
     1981 tgggtgagac tatggctaat caatcaacag cagttgtaga taatgcaact aattctttgg
     2041 gtgagactat ggctaataaa tcctcagcag ttgt
//
LOCUS       DASVEV010000317         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_372450, whole genome shotgun sequence.
ACCESSION   DASVEV010000317 DASVEV010000000
VERSION     DASVEV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372450"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VF222_13820"
     CDS             complement(<1..1421)
                     /locus_tag="VF222_13820"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7143550.1"
                     /translation="MELPSRKTLEGPSRAPHRAMYKAMGLKDKDLDKPIIGISSTCNE
                     ATPCNIHLGKLAQFAKKGACDGNCTPREFTAIAVSDGIAMGHEGMKASLVSREIIADS
                     IEIMMRAHQYDGLVGIAGCDKSLPGTLIGMARLNLPSIFVYGGTIMPGNWHNKDVTIQ
                     DVYEAVGSFEAGKISIEELIQLENVACPSAGSCGGMYTANTMASISEALGMSLPGSAS
                     PPAESEKRNEMCYETGKTIATLIENDLSPKDIMTFEAFENAITIANALGGSTNAVLHL
                     LAIAKEIGVKLILKDFERIRKRTPHIVNMRPGGLYVMLDLDKIGGVPIVLKTLLKNGL
                     INGKTLTVTGKTMEQNLESFHLNSNVDEKILSPYENPIEKEGTIKILKGSLAPQGAVV
                     KIAGMKLTKFVGKAKVFETEENAFRAISKNRIVEGDVVVIRYEGPKGGPGMREMLAVT
                     AALVGQGLGEKVSMVTDGRFSGAT"
     gene            complement(1496..1684)
                     /locus_tag="VF222_13825"
     CDS             complement(1496..1684)
                     /locus_tag="VF222_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143551.1"
                     /translation="MAETIEIENFNVLFSFDCPKCEYHNDEISVADLTNGSVECGMCE
                     TIFKIEGIDTLKITATIE"
     gene            1721..2254
                     /locus_tag="VF222_13830"
     CDS             1721..2254
                     /locus_tag="VF222_13830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7143552.1"
                     /translation="MAIIFYTYLNLYSEYQEIKVILVMIEKVIMINNTDNYHCIFCSI
                     INGTSEGITVYDNNHFLVLMDKYPISNGHTLVIPKKHYDNLLSMPHEEVGKLYSLVSI
                     IAKAVVSAVSADGFNIGQNNGKSANQIIPHVHVHIIPRFKNDNSYGKWPTRYIAKEDE
                     LYDFSKKIKNQLNVLSI"
     gene            complement(2366..3637)
                     /gene="serS"
                     /locus_tag="VF222_13835"
     CDS             complement(2366..3637)
                     /gene="serS"
                     /locus_tag="VF222_13835"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7143553.1"
                     /translation="MIDPKNLRDNLDKIIDMLDKRNVKFPLNDLINLDKKRRELIASL
                     QLERHKKNNIANNIAVKKRENEDISTSIKQMEETGKRIKLLEEKISGNNSKFYSLMML
                     LPNYVHDSVPVGKDQTNNVIIRSHGTIKKFDYTIRNHIDIGEGLDLIDIEKAAKISGS
                     RFYFLKNDLVKLNHAIISFALDFIYEKGYTLLQPPYMIRKNAMEGAVILEDFKDVIYK
                     IQDEDLFLIGTSEHAMAAMHMDEILEGSKLPLRYAGFSPCFRKEAGAHGKDMKGIFRV
                     HHFDKVEQFVYSRPENSWQEHERMLNIAEEFYKKLGIPYRVVLLCSADLGKISSKTYD
                     IEAWIPAQKNYREIVSCSNCIDYQARRLRIRFRDKSNEETKLVYTLNSTLVAIQRTLV
                     AILENYQTTNGVMIPEILQSYMGGTQEIKSK"
     gene            complement(3630..3890)
                     /locus_tag="VF222_13840"
     CDS             complement(3630..3890)
                     /locus_tag="VF222_13840"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HEX7143554.1"
                     /translation="MKENHHNCRISVNIEIEFAKVKERDAILNTLLPDNINFPDELSM
                     EMSVNNNSLIINTSSTKIDTMINTINEILHHISLATKVIKHD"
     gene            complement(3871..>4681)
                     /locus_tag="VF222_13845"
     CDS             complement(3871..>4681)
                     /locus_tag="VF222_13845"
                     /inference="COORDINATES: protein motif:HMM:NF044564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143555.1"
                     /translation="YNISPFIEGVTWNKENCYKILKNSGINTHKNGKLRTFAEISEEE
                     KNIILDAITKFIFLSSRNNSNKEIIENISDELVNQLYEFVKEDNNSILHDGREFAFVI
                     NACIRNMKSKLAIGICLGERNNTLIETEQLTNSYKANIVNSLEKIFKEKWRLIDNGLF
                     VFINGEGLLTDDNVKEIVEILSESITFRKKWFFLRTLSNDNNYKFYSIKGNNCDLKFD
                     IDSILKQSSDIISQADRNISNIYTLKVSYSKIEEFTSIIRKGISQYERESS"
BASE COUNT         1458 a          838 c          587 g         1798 t
ORIGIN      
        1 gttgcacctg agaatcttcc atcagttacc atagatactt tttctccaag gccctgtcct
       61 actaatgctg cagtaacagc tagcatttct ctcataccgg ggccaccctt aggaccttca
      121 tatcttatta ctactacatc accttcaaca attctatttt ttgatattgc cctaaatgca
      181 ttctcttcag tttcaaatac ttttgcttta cctacaaact tggttaactt cattccggca
      241 atctttacta cagcaccttg tggcgctaga gatcccttta aaatctttat tgttccttct
      301 ttctcaatgg gattttcata aggactcaaa attttttcat caacatttga atttaagtgg
      361 aatgactcca agttttgttc cattgttttg ccagtaactg ttaatgtctt tccatttatg
      421 agcccatttt ttaatagtgt ttttaataca attggcaccc caccaatttt gtccaaatct
      481 aacataacat ataatcctcc aggtctcata ttcacaatat gaggagtacg ttttcgtatt
      541 cgctcgaaat cctttaaaat taattttaca cctatttctt tagctattgc taatagatgt
      601 aatactgcat tcgtcgatcc tcctaatgca tttgcaatag ttattgcatt ttcaaatgct
      661 tcgaaagtca ttatgtcttt cggactaaga tcattttcta tgagagttgc aatagtcttt
      721 cctgtttcat aacacatttc atttctcttt tcgctctctg ctggaggaga agcactacca
      781 ggcaacgaca tccctaaagc ttcgctaata gatgccatag tatttgcagt atacatccca
      841 ccacaagaac cagcagatgg acaagcaaca ttttccagtt gaattagttc ttctatactg
      901 attttgcccg cctcaaacga accaacagcc tcataaacgt cttggattgt tacatcttta
      961 ttgtgccaat ttccaggcat tattgtccct ccgtaaacaa aaattgaagg taaatttaat
     1021 cgagccatgc caattaatgt acctggcaaa ctcttgtcac aacctgcaat tcccactaac
     1081 ccatcatatt gatgtgctct catcataatt tcgatcgagt ctgctattat ttctctacta
     1141 actaacgatg ctttcatacc ctcgtgtccc atagctatac catcagatac tgcaatagca
     1201 gtaaactctc ttggagtaca attcccatca catgctccct tctttgcaaa ttgtgcaagt
     1261 ttacctaaat gtatgttaca tggtgtagcc tcattgcaag tagaagatat accaataata
     1321 ggtttatcca aatctttgtc ttttaatccc attgctttat acatagctct gtgaggtgcc
     1381 ctagatggtc cttcaagagt ttttctactg ggtaattcca ttaaaataag aatacaaaag
     1441 tattatttta agagtctttc ctccagaatt atataagaca gagcataaat attgtttatt
     1501 ctatagtagc ggtaattttt aatgtatcaa tcccttcaat cttaaagata gtttcacaca
     1561 ttccacactc cacactccca ttagtcaaat cagcaacaga tatttcatca ttatggtatt
     1621 cgcatttagg acaatcaaat gagaaaagta cattaaaatt ctctatctca atagtttctg
     1681 ccattatgta agagatagga ggaaatagct atatttaaaa ttggctataa ttttttatac
     1741 ttatctaaat ctgtattcag aataccaaga aataaaagta atattagtaa tgatagaaaa
     1801 agttattatg attaacaata ctgataatta tcattgtatt ttctgttcaa taatcaatgg
     1861 aactagtgaa ggaataacgg tttatgataa caatcatttt ttagtattga tggataaata
     1921 tccaattagt aatggacata ccttggtgat tccaaaaaaa cattatgata atttactttc
     1981 catgcctcac gaagaggtag ggaaattata ctctcttgtt agtattatag ctaaagcagt
     2041 agtttcagca gtttctgcag atggatttaa tataggacaa aataatggaa aatcagcaaa
     2101 tcagattatt cctcatgtac atgttcatat tattcccaga tttaaaaatg ataattctta
     2161 tgggaaatgg cctacccggt atattgcaaa agaagacgaa ttatatgatt tctctaaaaa
     2221 gataaaaaat caattaaatg ttttgtctat ttagattagt taaatataac tcattattct
     2281 tgatttttat ttatcttcat aagataattt taatatgtct ttattattgt acttatctta
     2341 agataaatac acacatatag atgatttatt ttgatttaat ttcttgagtt cctcccatat
     2401 agctttgcaa aatctctgga atcattactc cattagttgt ctgatagttt tctaaaattg
     2461 ctactagcgt tctttgaatt gctaccagtg tgctatttaa tgtataaact aatttagtct
     2521 cttcatttga tttatctcta aaccttattc ttaatcttcg agcctgataa tcaatgcaat
     2581 ttgaacatga aactatctct ctataatttt tctgtgctgg tatccatgct tcaatatcgt
     2641 atgtcttaga tgatattttg cctaaatcag cagaacataa tagaactact ctgtatggta
     2701 ttccgagttt tttataaaat tcctctgcaa tatttagcat tctttcatgt tcttgccaag
     2761 aattctctgg tctagaatag acaaattgtt ctaccttatc aaaatggtga acccggaaaa
     2821 tacctttcat atcttttcca tgagctcctg cttctttcct aaaacatgga ctaaatcctg
     2881 catatcttag aggtaattta gaaccttcta atatttcatc catgtgcatt gcagccatag
     2941 catgctcaga agttccgatc agaaagagat cttcatcttg aattttatat attacatctt
     3001 taaaatcttc taaaattact gctccttcca ttgcattttt tctgatcata tatggtggtt
     3061 gtaacaatgt atatcctttc tcataaataa agtcaagagc gaaacttatt attgcatgat
     3121 ttagttttac taaatcattt tttaaaaaat aaaatcgtga tcctgatatc tttgcagctt
     3181 tttcaatatc aattaagtcg aggccttctc caatatcaat atggttcctt attgtataat
     3241 caaatttttt gattgttcca tgacttctaa ttatgacatt gtttgtttgg tcttttccaa
     3301 ctggaacaga atcatgtaca tagtttggaa gcaacatcat caaactataa aattttgaat
     3361 tattaccaga gattttttct tccagtaatt taattctctt ccctgtctct tccatttgct
     3421 taatagaagt tgaaatatct tcattttccc tttttttcac cgctatatta ttagcgatat
     3481 tatttttttt gtgtctctct aattgtaagc ttgctattaa ttctcgacgt tttttatcta
     3541 agtttattaa atcatttaaa ggaaatttta catttctctt atctaacata tctataattt
     3601 tgtctaaatt atctcgtaga ttttttggat caatcatgct ttatcacttt tgtagcaaga
     3661 gaaatgtgat gtagaatctc atttatggta tttatcatgg tatcaatttt agtagaagat
     3721 gtattaatta tcaacgaatt attatttact gacatttcca tagaaagttc atcgggaaaa
     3781 ttgatgttat caggcaataa agtattcaaa atggcatctc tttcctttac ttttgcaaac
     3841 tctatttcaa tattaactga tattctacaa ttatgatgat tctctttcat attgtgaaat
     3901 tccttttctt ataatagatg tgaattcttc aatttttgaa taagatactt ttagtgtata
     3961 tatattagaa atatttctat cggcttgaga tataatatct gaggattgtt ttaaaatgga
     4021 atcaatatca aattttaaat cacaattatt accttttata ctataaaatt tataattatt
     4081 atcatttgat aaggttctta aaaaaaacca tttttttcta aaagttattg attcacttaa
     4141 aatttcaaca atttctttta cattgtcatc cgttaaaaga ccctccccat taataaatac
     4201 aaataaacca ttatcaatca aacgccattt ctctttaaat attttttcta atgagttaac
     4261 aatattagct ttataactat tagttaattg ttcagtctct atcaatgtat tgtttctttc
     4321 tcccaagcat attccaattg ctaatttaga cttcatattt cttatacaag cgttgatcac
     4381 aaacgcaaat tcccttccat catgtaatat actattattg tcttctttta caaattcgta
     4441 taattgattt actagctcat cggaaatatt ctcaataatc tctttgttac tattatttct
     4501 acttgataga aaaatgaatt tagtgattgc atctaaaatg atattttttt cttcttcaga
     4561 tatttctgca aaagttctca atttgccatt cttatgagta ttaatccctg aatttttcaa
     4621 aattttatag caattttctt tgttccatgt aacaccttct ataaatggtg aaatattata
     4681 a
//
LOCUS       DASVEV010000318         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1348024, whole genome shotgun sequence.
ACCESSION   DASVEV010000318 DASVEV010000000
VERSION     DASVEV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348024"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..515)
                     /locus_tag="VF222_13850"
     CDS             complement(369..515)
                     /locus_tag="VF222_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143556.1"
                     /translation="MPQKLETVLKHVEDIGNDVNRQLIHEYHNYLVSRDTSISITKYQ
                     NDKR"
     gene            complement(665..1522)
                     /locus_tag="VF222_13855"
     CDS             complement(665..1522)
                     /locus_tag="VF222_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143557.1"
                     /translation="MYCPTLDRLISFLQSSRRVARHFCLYFKIYSIDYGVVDLALAGD
                     NYQLDIFGSAVNICSKINSSSLSILNELIIGNNFYRILKSFSNIIDKYNFVNNGGFKL
                     TENIEYPTYTIKRRSNTLLQNNDTNSTHSMMKKQSNSYAEEDFIENRNNKREGFFFHK
                     KKKNKRIIIVDDDQEILFTYRLFLEGYNYHIISFTDGFDALNCIKDLPNFDDLLVILD
                     IRMETLNGFELHQQIKAIDPTIKILFITVLDILDEILTIVPGITREHIMRKPVDKKIF
                     TNTVKKILN"
     gene            1708..2181
                     /locus_tag="VF222_13860"
     CDS             1708..2181
                     /locus_tag="VF222_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143558.1"
                     /translation="MNNTIVFVSIFIAFLALLTFYSPVTTNAQKVIENHEDNTEKLNN
                     GIIDTIPKSCEDSFLSKNEGGPLSQDVLSALNMYLSHNPVAMGDEPLTNVKNVDGLCV
                     LIKLVDSESTQGRVANEEQVRNILNAALQEKRNLSVIEGIIDNLLSLNLIQPSDD"
     gene            2620..>2881
                     /locus_tag="VF222_13865"
     CDS             2620..>2881
                     /locus_tag="VF222_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143559.1"
                     /translation="MINKLLNMNLIYLKYVFLFIFLSIMIITVTYENYAFAATYNYAP
                     FFPTTGSNKLDVPNASNLRLSSFTVATWFKTTANFPDEGVLVN"
BASE COUNT         1036 a          399 c          367 g         1079 t
ORIGIN      
        1 ttacatggta atgatgaaca ttcagaatca ggaccataaa gaaaagagtc ttgtctaacc
       61 caatcgttag gatattcaaa ctttactcct atttcagaat cttcaaaaat tagagaatca
      121 gcattaggtt gaggagtaga aaaagctata ttattagtat ttaatattat tagcacaata
      181 attatcataa tcataatttt tgttaatatt atattagatg taacaaagtg aaatctcatt
      241 ataactaaag gggtatattt ttataaatat gtttgatatt aatgactttc tttgttcttc
      301 taataaaaaa gtgacgaaag cgaaaatgtt ctaatatcat tgtattttta ttaatgtagt
      361 attgactgtt atcttttgtc attttgatac ttggtaatgc ttatgctggt atctctagag
      421 actaaataat tatgatattc atgaatcagt tgtctattga catcattgcc aatgtcttcg
      481 acatgtttca gaacagtttc aagtttttgc ggcattagac gtctctcatt tctattttac
      541 tattgcaaca aacttgtata tgaaccccta tataatacgg gctcggaggt gtgtgaactg
      601 atttcgacac aattttgtag ttcagaaaaa atatatgaaa atactaaaaa aataataata
      661 tttgttaatt taatattttt tttacagtat ttgtaaatat ttttttatca acaggttttc
      721 tcattatatg ttctcttgtt attcctggaa ctatagttaa tatttcatct aatatatcta
      781 aaacagtaat aaaaagaatt ttaattgtgg gatctatagc ttttatttgt tgatgtaatt
      841 caaatccatt taaggtttcc attctaatat caagtattac tagtaaatca tcaaaatttg
      901 gaagatcttt aatacaatta agtgcatcaa aaccgtctgt aaaagaaatt atatggtaat
      961 tgtaaccttc tagaaaaagc ctatatgtaa aaagtatttc ctgatcatca tcgactataa
     1021 ttattctttt gtttttcttt tttttatgga aaaagaaacc ttctctttta ttattcctat
     1081 tttctataaa atcttcttca gcatatgaat tagattgttt tttcatcatg gagtgtgtac
     1141 tgttagtatc attattctgc aacaaagtat tacttcttct ctttatggta tatgtaggat
     1201 attcaatgtt ttctgttaac ttgaatccac cattatttac aaaattatat ttgtctataa
     1261 tattagaaaa agattttaaa attctataaa aattatttcc aataattagc tcattaagaa
     1321 tagacaaaga agaagaatta atctttgaac atatatttac tgctgaacca aatatatcta
     1381 gttgataatt atctccagct agggctaagt ctactacacc ataatctata ctataaattt
     1441 taaaatagag gcaaaaatgc ctcgcaaccc gacgagatga ttgcaggaat gatattaacc
     1501 tatcaagagt tggacaatac aataaattac gatattatta aaagtagtct ttattttttt
     1561 taattaaatt aaattcaata ttttagattc ataaaaattt tattctaaaa attgaaatat
     1621 tatacaatca tattaaaaat gcatttaaga atctatcaga acctaaatat attaagattt
     1681 aatctaaata gatataagtt gaagaacttg aacaatacta ttgttttcgt ttcaatattt
     1741 atagcatttc ttgctttatt aacattttac tcaccagtca ctacgaatgc acaaaaggta
     1801 attgaaaatc atgaagataa tactgaaaaa ctgaataatg gcattataga taccatacca
     1861 aaaagctgtg aagattcttt tttgtcaaaa aatgaaggag gaccactttc ccaagatgtt
     1921 ttatcagctc tcaatatgta tctttctcac aatccagtag caatgggtga cgagcctcta
     1981 acaaatgtaa aaaatgtaga tggtttatgt gtattaatta agttagttga ttcagaaagt
     2041 acacaaggaa gagtagctaa cgaggaacaa gtaagaaata tacttaatgc agctttgcaa
     2101 gagaagagga atctttctgt aatagaaggt ataatcgaca atctattatc tttaaatttg
     2161 attcaacctt ctgatgacta attttttata agataaaatt ttttattttc cttacatttg
     2221 tttagataat atgtagagta aaataagatc ctttctcatg ttctaatctc ttttatatca
     2281 acttatacaa taatatattt cgtatattaa aaaataatat gatgtttcat aacatattac
     2341 gcttaggtat ttaaaataat ttgagattag tagtattaaa tagatgtatt catgtaccaa
     2401 taatctatca cttcttacat ttgacaggat catttataca aaattacaac aaatcattat
     2461 tatactgaat tctattgatt tccattgtgc acacaagttt aggaaattaa tgaatctgtc
     2521 tttccttgtc aatcttcata atacattgaa aggtgctaag aacataatgt tcaatctgac
     2581 catcttacta acacaaaata aaaaaatagg tgaatacacg tgataaataa actccttaat
     2641 atgaacttaa tttatctaaa atatgttttt ctttttatct ttctttctat tatgattatc
     2701 accgtcacat atgaaaatta tgcttttgcg gccacttata attatgcacc attctttcct
     2761 acaactggtt caaataaact ggatgttcct aatgcttcca accttcgatt atcttcattt
     2821 acagttgcca cttggttcaa gacaacagcc aacttcccag atgaaggtgt tttggtaaac
     2881 a
//
LOCUS       DASVEV010000319         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1152418, whole genome shotgun sequence.
ACCESSION   DASVEV010000319 DASVEV010000000
VERSION     DASVEV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152418"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF222_13870"
     CDS             <1..660
                     /locus_tag="VF222_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX7143560.1"
                     /translation="ANKSQSLLIILQGISAAGKDSTIRNVMSAFNPQSCKAISFKAPN
                     EDEISHDYLWRVHKLMPAKGEITVFNRSHYEDVIEVRVRKLISKSIWSKRYIQINGFE
                     RFLYENNTRIIKFFLYISKDEQKKKLEERIKNPLKQWKITESDLTESTYYEQYMEAYE
                     EALWKCSTDWAPWYIIPANTKWFRNFVVAQIIVNTLESMKLTFPKPNFDISKYLRMYK
                     Q"
     gene            917..1573
                     /locus_tag="VF222_13875"
     CDS             917..1573
                     /locus_tag="VF222_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX7143561.1"
                     /translation="MTRLLDLGLERITEIIMNMAKLSEMCVINAIESYENGIVEKNQI
                     FEWSEKLKVFQLEVSDLATELIFRYQPVATDLRYIRSCMEIAYGFSRFGRYAYDIVEV
                     LEIMGSIHKCDKSVVMETSNLASEMIRLSINALQTKEKNTVSKLYKMDDSVDALYRKY
                     LRDLITLKADSNDHPLKDPRCAISALLIMRYLERIADHACYIGDSVYFIVTGQSSPRK
                     "
     gene            2619..2867
                     /locus_tag="VF222_13880"
     CDS             2619..2867
                     /locus_tag="VF222_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143562.1"
                     /translation="MNILEIEFPLKIEAKTSKYNEKTNVIYSIAELQHNFCIDKKEII
                     IEQIKACENLSRYITNKSDRLALEREISELKLALDILY"
BASE COUNT         1399 a          439 c          486 g         1209 t
ORIGIN      
        1 gcaaataaaa gtcaatcatt gctaatcatt ttacaaggga tatctgcagc tggcaaagat
       61 agtactataa gaaatgtaat gagtgctttc aatccacaaa gttgtaaagc catatcattc
      121 aaggctccaa atgaagatga aatttcacat gattatttgt ggcgtgtaca taaattaatg
      181 cctgcaaaag gtgaaataac agtatttaat cgttctcact atgaggatgt tatagaagta
      241 agagtacgta aattgatatc aaaatctatt tggtctaaaa gatatataca gattaacgga
      301 tttgaacgct ttttatatga aaataacacc agaatcataa aattcttttt gtacataagt
      361 aaagatgaac aaaagaaaaa attggaagag agaataaaaa atcctttaaa acaatggaaa
      421 attacagaaa gcgatttaac tgaatccacc tattatgagc aatacatgga agcatatgaa
      481 gaagctttat ggaaatgcag tacagattgg gctccgtggt atataattcc agccaataca
      541 aaatggttta gaaattttgt agtagcacag attatagtca atactcttga aagcatgaaa
      601 ttaacttttc caaagccaaa ttttgatata tccaagtatc ttcgaatgta taagcaataa
      661 tatggaagta ctgacattag ttatacaatt cttaaatagg ttgactttct ttggtcaata
      721 ctgttgagac agttcgcttt catctcatta taaaacctgg ataaagtttg taaatataag
      781 gagtaactag agaacaaatt ctcattatat atatcatgat tgaaattatt ttcaaataag
      841 atcacaatta gatattggtc taagatatac cgtattctta atttatattt ctagtattat
      901 aataatacat tgtacattga caaggttact cgatctggga ttagaacgta taacagaaat
      961 aataatgaat atggcaaaac tttctgaaat gtgtgttatt aatgctattg aatcatatga
     1021 aaatggtatt gttgaaaaaa accaaatatt tgaatggtct gagaaactta aagtatttca
     1081 attggaagta tctgatctag caaccgaatt aatttttagg tatcaaccgg ttgcaacgga
     1141 tttacgatat ataagatcat gtatggaaat tgcttatgga ttttctagat ttggcagata
     1201 tgcatatgat attgttgaag tactagaaat aatgggctcg attcataaat gtgataaatc
     1261 tgtggtcatg gagacttcaa atttagcaag cgaaatgata agattaagta taaatgcatt
     1321 acaaactaaa gaaaaaaata ctgtttcaaa gctatacaaa atggatgatt ctgtagatgc
     1381 gctgtacagg aaatatctaa gagatttaat tactctaaaa gcagactcta atgaccatcc
     1441 acttaaagat ccacggtgtg cgatatctgc attattgata atgcggtatc tggaaagaat
     1501 agcagatcat gcatgctata ttggagattc agtgtatttc atagtgactg gacaatctag
     1561 tccgagaaaa taaaattttt gcagtcaaca acagcaaagc attggagctg tatttgatac
     1621 tgattaatct tattatttgt agcagataat acttttacta acaatagaaa attccatttt
     1681 acatttttta acattctaag gcaaaaatct tttacagcta cactcactgg aattacattc
     1741 atctaaatta gagtgattat atttttcatg accacaggaa catagatcag gataaacaaa
     1801 gcaactattt tttacattag aataaacatc ataataacta tttataaaaa atcttctttt
     1861 tagtgacata atccctcatt taggttagta ggatcaatta tactcccttt attagtataa
     1921 catttccata catgtgtttt tgcactcatt ctaagccact attctctaat acatatccat
     1981 atagtagaaa aaaataatta aatatattgt cattactcta tatagcaaat aagaaaaaaa
     2041 cgacacttaa attatactat ataaaggaac atagtttatt gttttatata atcctatatt
     2101 gttacaatac tatataagac ttagaaagaa atattaattg tcataatagt tcacataaaa
     2161 aagtatattc aaaatataaa tatatcaagt gactattata aatgaagatt cattctatcc
     2221 tatgcaaagt aggtcaggag caagcgaata gttatcgctc ctttctctta ctcaggaaca
     2281 ttataagatt caatatttta tgatatgaaa aaaatcattc taattttttg actattaaaa
     2341 attaaaaaaa ataatgataa atcttaaagt tcttctaaca tggaataata atggaatgat
     2401 gcgaatgttt atgaatgaaa tataaacaag aatttctaaa aattatttta aaatagtaat
     2461 agaaaaccat aataaagttc tcataatttt tttctcttcg ttaaagtaaa gacaaataat
     2521 gaaatatgaa gttcctagtc tttctcttat gtctaaatag ttcatttact aaaaaataaa
     2581 ataattcaat gtataacttc ttttagtgat ttgaaataat gaacatatta gaaatagaat
     2641 ttccattgaa aatagaggct aaaacatcaa aatataacga aaaaacgaat gttatatatt
     2701 caattgctga attacagcat aatttttgta tagacaaaaa ggaaataata atagaacaaa
     2761 ttaaagcttg tgaaaacttg tctagatata ttactaacaa aagcgacagg ttagcccttg
     2821 aaagagaaat ttctgaatta aaattagctc tggacatttt gtattagcta ttaaaatatc
     2881 taaaatattt ttattaatat acttatcata ctctatatat gtagaattga tttatgttgc
     2941 ttttatttaa tactcatcaa cagatactcc caattcatat acttgtaatt ggtatcgaaa
     3001 aatttttatt ttgatattct atttctagtg gtattctaac agatttatga aataatgaaa
     3061 ccaattaagt tcaaataata tcggcattat atggttatga ttacaaaggt tattgtattt
     3121 tgaaatataa ataattgctg taaaatataa tttcatatta ttaaaattct ataaaattca
     3181 ataatatcaa atatctttct atcaataggt ttacaataat tatatttaat ttttaataat
     3241 gtattgtata aaaaattctt ttgtcaaaat aatattttac ttatttattt tcatatattt
     3301 atgtcaacat ataataccgt aaaaccattt taaagcagat aaagatttaa gttaataagt
     3361 tgttttattt agtaatgaaa actttattac ttcttcgtca tgctaaatca agttggaagg
     3421 atactacact tgttgatcac aaaagaccac taaataaacg tggtaaaaaa gatgcaccaa
     3481 taatgggtga atatttgaaa aataaaaagt taactcctga tttaattatt agt
//
LOCUS       DASVEV010000320         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_959669, whole genome shotgun sequence.
ACCESSION   DASVEV010000320 DASVEV010000000
VERSION     DASVEV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959669"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..947)
                     /locus_tag="VF222_13885"
     CDS             complement(471..947)
                     /locus_tag="VF222_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7143563.1"
                     /translation="MVFKTMLVPYDGSTMSDKALDKAVELANIIKESRIMIIHVVPEI
                     PTPIFSRVIRSPKTGEVITFSEYLVSLYQEMESKMREKLEERKEKYSKYGFPIEIYIT
                     TGIPTDKIIEYATDKKVDLIVIGSIGVTGISKFFKGLGSVSRNVSEKVSCPVLIVR"
     gene            complement(1014..1256)
                     /locus_tag="VF222_13890"
     CDS             complement(1014..1256)
                     /locus_tag="VF222_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143564.1"
                     /translation="MGKKKKEIKIIKNDSNKGDYTFVSDDILNNVKDNESTNNEKKPE
                     SKPGEIIEKKIHSKKVDNKGRKILGKSLQGHSPVYE"
     gene            2120..2704
                     /locus_tag="VF222_13895"
     CDS             2120..2704
                     /locus_tag="VF222_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7143565.1"
                     /translation="MSSEVRINESPRHFMVLDAISRGIQDPNKIAKVLKINKEEVELI
                     VNDLLTQRLIIKKENRGFFGNKKIKISITDTGIRLLKSKKEELERKWHQAQKMYNNGK
                     GNKAQLQTYMETNRSWVPLMLFSGIIDVLFFMTMMSFLGMALNPMEQSMAGESGAEDA
                     GAAESSTGETSGSGADSGDTGDFGGFDAGGFGDF"
     gene            complement(2803..3306)
                     /locus_tag="VF222_13900"
     CDS             complement(2803..3306)
                     /locus_tag="VF222_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143566.1"
                     /translation="MSSRKKIKIELEDDEGSKYNLSLEGNFSKEKILQVIELMDLVRS
                     SDSGAITNNPLYEKNNTLSVDSKIWNIVEESFYFNTFTSSDIVNIYKKKYNETIKLSI
                     ISTYLARYCVKGKLNRTKRLREYVYNLVRNSSTNADSINNVVSSDDDDNKIAYDVRRK
                     TIDDLKN"
     gene            complement(3457..3753)
                     /locus_tag="VF222_13905"
     CDS             complement(3457..3753)
                     /locus_tag="VF222_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX7143567.1"
                     /translation="MEFSFLYSSSLLAFIIGIAATVLTRYSSIPQIIKGFKTKRMDDV
                     SFWLIFYLTVGLFLYVIYGIIIDDAIIIWGNAIGVISNLILIILKIKYSVKSFE"
     gene            complement(4254..>5124)
                     /locus_tag="VF222_13910"
     CDS             complement(4254..>5124)
                     /locus_tag="VF222_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX7143568.1"
                     /translation="MGFGGFAKTIFEQMFGGRGGRAGGFEGSTDPFSGFSFNIGGNRR
                     KGRDILYDMELSLEDVLRGKKDEIELPKFESCNDCGGTGAAHGTKPRTCTTCNGQGQT
                     RRVYNQNRFSTFISLEPCQTCNGKGQMIDKPCTSCLGSGKIKQNKKLKINIPSGVEDG
                     MTLQLTGEGEYSEQGPPGDLLIRLHVLPHNSFERLEDGHVLHDLNLDYPSLVLGTEIK
                     VPTLHGYEKLKIPAGTQANTILKIKGKGLPRYGTYGKGDQLVRILVKIPNNITEKQKL
                     LLKELNKEFSKYE"
BASE COUNT         1772 a          763 c          702 g         1887 t
ORIGIN      
        1 atcaggaatg tggatctgat cttgttcttc tttctgtgat gctactactt ttttcgcatt
       61 tttactttta tctttgttga acatctcaaa atcttctttt actcttccta tggctttcaa
      121 atgaatccat tagatattaa ccttaggtaa taataaagaa aatataaaga ttatgcatat
      181 atttaatata caattttatt cttttatcta taaattcact taattatttt tccaatatta
      241 gctatagaat actaactaga tcttacaaat cgaaaatcta ggaattaaga tattgttatt
      301 atctaaatag ttatttagta atactgtaca aaaatttaaa ttaaaatcta tcccttattt
      361 catatagtac ctaagttttg aaagattatt aagttactct atgtaatatt attttacatt
      421 ggacaaatta taattaatag taaaaagaac aaagatttga tatatgtagt ttatctaaca
      481 atcaaaactg gacatgaaac tttttcagag acgtttcttg aaacacttcc caaaccttta
      541 aaaaattttg atattccagt gacaccaata cttccaatta caataaggtc aactttttta
      601 tctgttgcat attctattat cttatcagtt ggaattccag tggttatgta tatctctatt
      661 ggaaatccat atttgctgta tttttctttt ctttcttcta gtttttctct cattttggat
      721 tccatttctt ggtataaaga taccagatat tctgaaaaag taattacttc cccagtttta
      781 ggagaacgaa ttactcttga gaaaattggt gttggtattt ctggaacaac atgaataatc
      841 attattcttg attcttttat tatattggct aattctactg ctttatctaa agccttatca
      901 ctcatagtag aaccatcata tggaacaagc attgtcttaa ataccaaagt attactgcta
      961 taacatcctc aaattataaa taaaaatgta atgtagatac atctgcatta tgactattca
     1021 tatactggtg aatgaccttg caaacttttt cctaaaatct ttctgccttt gttgtctact
     1081 ttctttgaat gaatcttttt ttcaattatc tcaccaggtt tactctctgg ctttttttca
     1141 ttatttgtag attcattatc ttttacatta tttagaatgt catcgctaac aaaagtataa
     1201 tctcccttat tagaatcatt ttttattatc ttaatctctt tttttttctt acccattcct
     1261 ttaacctttt tttttattta caaattatca atcagtatta tttccaaaat aaatgaccaa
     1321 tgaattaggg aattgcaaat tatttgtgaa ataacattaa atgtgatata ttaagaaaag
     1381 cactcaatat cagggattga taaaatgcaa tactttttgt ttaaagatat taaatataaa
     1441 gtttgttaag ccaactcata aataaatata atttataaag tgttttgaaa tttattgaat
     1501 aggaattcgt tagcaattta caggagggag taataccgat agcggttcta aaataagagt
     1561 ctaatgttat agtaataaag aaaataatat ttaagattta cttttcagtt attacatgga
     1621 aatttctaaa agatttaatt aaaaaagaaa aagttaaggt cgcaatggtg aattgttaaa
     1681 acatatggct gtacttgaga agttaacttg accttgtatt gtagtactat aagcaacaaa
     1741 gatatctatt tcatctcgtt gaataaacca aggtccagaa gcccctgaga cgtcaaaaaa
     1801 tccatctata ataatgtcgc cggacgtgga tacaacaata ttctatacaa tcagtgtaac
     1861 aaagatgaaa ctattgtatc agtcataaaa agactgttta ggtaacatat tacatccggg
     1921 ttgactagaa tgcagaacag aaaatgatct taggtgtata gtatacaatc ttcatagaat
     1981 ggtgaagttg gtgttatgac gatggtttct acacagccca attcaatatt tgttttattt
     2041 acaattagat taggaacatc aatttaatat tgaattctta tcataaaagg atttaactaa
     2101 gtataacata ttataaggag tgtcttctga agtaagaatt aacgaaagtc cacgtcattt
     2161 tatggtactt gatgcaatct ctcgagggat acaagatcca aataaaattg caaaagttct
     2221 caaaattaat aaggaagaag tagaattaat agtaaatgat cttttaactc aaaggctgat
     2281 cataaaaaag gagaataggg gtttttttgg taacaaaaaa attaagattt ctattacaga
     2341 tacaggaatt agactcctga aatccaaaaa agaggaacta gaacgaaagt ggcatcaagc
     2401 tcaaaagatg tataacaatg ggaaaggaaa taaagctcaa ttgcaaacat acatggaaac
     2461 taatagatca tgggtaccgt taatgttatt ttcaggaata atagatgttt tattctttat
     2521 gactatgatg tcttttttag gtatggcatt aaatccgatg gaacaatcga tggcaggtga
     2581 aagtggcgca gaagatgcgg gagcagcaga atcaagtacc ggagagacaa gcggtagcgg
     2641 agcagatagt ggagatacag gagattttgg cggatttgat gcgggaggat ttggagattt
     2701 ttgatttcca aaattatgtt acagaatggc gatagcctaa cttgatagaa aactatcgtt
     2761 attcaatagt tatttttaat aaatatttaa attttttata aattaatttt taagatcatc
     2821 tattgtcttt cttctaacat cataggcaat tttattatca tcgtcatcag atgaaactac
     2881 attattaata gaatccgcat ttgttgaaga gtttctcact aaattataca catattctct
     2941 tagtctttta gttctattta atttaccttt tacacaatat ctagcaagat aagttgatat
     3001 aatgcttaat ttgattgtct cattatattt ttttttataa atattaacaa tatcagaaga
     3061 agtaaatgta ttaaaataaa acgattcctc cactatattc caaatctttg aatcaacaga
     3121 taaagtatta tttttttcat acaatggatt attagttatt gctccagaat ctgatgaacg
     3181 tacaagatcc ataagctcaa taacttgtaa tatcttttcc tttgaaaaat tgccttccaa
     3241 tgagagatta tacttactcc cttcatcatc ttccaattca atctttattt tcttcctcga
     3301 gctcaaagta aaagatcttt attttgtaag ccatttattg cttttaaata taagagtaaa
     3361 ttaaaattaa gtaattgcta aactttgtta atagatgaac gtctattaat tagaaagctg
     3421 cttgatataa ctaaagtatg acatatttaa caatcattat tcaaatgatt tgacagagta
     3481 tttgattttg agaataatta gtattagatt tgaaattaca cctattgcgt ttccccaaat
     3541 aattatagcg tcatcaataa ttattccgta aattacataa agaaataatc ctactgttag
     3601 ataaaatatt agccaaaatg acacatcatc cattcttttg gttttgaatc cttttattat
     3661 ctggggaatg ctactataac gggttaaaac tgtagctgca atacctataa tgaaagctaa
     3721 taagcttgat gaatataaaa atgaaaattc cattatcatt gataatcttt atacggatat
     3781 tttattagta gtattgaaaa tttaattaac cgacaaatca aaaagatttg tttaaatttt
     3841 aaaagttaga tttaaagtag catataataa acaaattaag gtatataaaa catttctata
     3901 gatatctagt catgactatt ttgtatgctt aaataaaaac gatatgttat ttagtgttaa
     3961 cctatgttga tttgtaaacc tttactagtc gaatttttct tgactgattt taacggaata
     4021 gtcacaggca atagaccaaa ttttttatat tctatccatc gtcttttatt taagttcttt
     4081 tatgatttgg aactagttgt taaaaatact aaaaaatctc ctatatcaat tttcttttca
     4141 cacattatac tatcaatatc aagaaagcaa ttttcagatc gttttttcaa aaaaattcat
     4201 cgaatgggta tgtcatgtaa cgtagggtta aaattatttt gtatatgaaa aagttattca
     4261 tattttgaaa attctttatt taattctttc aaaagtaatt tttgtttttc tgtaatatta
     4321 ttaggaattt tgaccagtat ccttactaat tgatctccct tgccatatgt tccataccgt
     4381 ggtagtcctt ttcctttaat ctttaaaatg gtatttgctt gagttcctgc aggaattttt
     4441 aatttctcat aaccatgtaa agtcggaact ttaatttcag tacctaatac taaactagga
     4501 tagtcaagat ttagatcatg taatacatgg ccatcttcta atctctcaaa cgaattatga
     4561 ggcaaaacat gaagtctaat taagagatct ccaggaggcc cttgttccga atattcgcct
     4621 tctccagtta gttgcaaagt cattccatct tcaacccctg atggtatatt tatttttaat
     4681 ttcttatttt gttttatttt acccgagcct agacaactag tacaaggttt atcaatcatt
     4741 tgacctttac cattacaagt ttgacatggt tctaacgaaa taaaggtgga aaaacgattt
     4801 tgattataca ctctacgagt ttgaccttga ccattacaag tagtgcatgt acgtggttta
     4861 gttccatgag cagctcctgt tccaccgcaa tcgttacaac tttcaaattt aggtaattca
     4921 atctcatctt tttttcctct gagaacatct tctaaagaga gttccatgtc atagagaata
     4981 tccctacctt ttcttctatt acctcctata ttgaaactaa atccactaaa gggatcagta
     5041 gatccttcga atcctcctgc tcttcctcct ctaccaccaa acatttgttc aaaaatagtt
     5101 tttgcaaagc caccaaaacc cata
//
LOCUS       DASVEV010000321         9597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_183008, whole genome shotgun sequence.
ACCESSION   DASVEV010000321 DASVEV010000000
VERSION     DASVEV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183008"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF222_13915"
     CDS             <1..233
                     /locus_tag="VF222_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7143569.1"
                     /translation="RDGFNRGYDVILISDATASTNFRHYKTTIEHVKEYYGLVMKAKE
                     MERLMEANKSGGYLEKIKMIREKRNLLEPDHN"
     gene            complement(439..585)
                     /locus_tag="VF222_13920"
     CDS             complement(439..585)
                     /locus_tag="VF222_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143570.1"
                     /translation="MEGIQKLKCSSCGSTFMSETGMAKCPSCLEKESQGAGNTAGCGC
                     GHQH"
     gene            1037..1600
                     /locus_tag="VF222_13925"
     CDS             1037..1600
                     /locus_tag="VF222_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HEX7143571.1"
                     /translation="MNINKKGIRVLAIAESFKKEKSNSILTGVVMRKDLIIDGIIFGK
                     VTIGGNDATQNILAMYNNLQRNDIGFILFDGMIISMFNIVDGETIHNKTGLPVIIITF
                     KNSEGIHNSIKKYFPDTYKTKITLIEKLGKREPVLLKTNKILLIRTWGIKLDDAVRLL
                     NSFLIQGSIPEPIKIAKIISRAYLNTL"
     gene            1745..1963
                     /locus_tag="VF222_13930"
     CDS             1745..1963
                     /locus_tag="VF222_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143572.1"
                     /translation="MNEQTGLDNTTYDILSALGREADFLYDTIDKYIDDAKQSNNDEL
                     VDLWNRIKQDRLKHINDLKEALERVIHK"
     gene            complement(2287..3258)
                     /locus_tag="VF222_13935"
     CDS             complement(2287..3258)
                     /locus_tag="VF222_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX7143573.1"
                     /translation="MTTTPELLHTGISEKIADDCPNCRNPHIIFDSSNGEMICTSCGM
                     IVEDRLDSSGIESEPMKNIENNLSVGMPLSLAHYDKGLSTTISSSNVDAFGSQLNTSQ
                     MAKVRTIRHWNKISSNNRSYHRNLKNAFGVLLRIKDKLSLSDTIIEKSAYYYRKVVEK
                     RITKGRSIKEFVVACVYVACREMNIPRTLDEIAEIANADKVFAGKCYRLLLRNLKIRL
                     QPIDSTTYLSKIANKGGISKKTLHRAIEMMAEIKEHPFSSGKDPTALSIAVLYAACLE
                     QGENISQSQVAIAGEMSVVTLRKRFLDVKIIFPHIPNNTIELRKRKT"
     gene            3387..3905
                     /locus_tag="VF222_13940"
     CDS             3387..3905
                     /locus_tag="VF222_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143574.1"
                     /translation="MPNSTRSFRVEQYISNILDEEAERMGISVNALVNIILKHYSDFS
                     RFLSKIDLVILNREILTKFLENMDDQDIFKIGLELGETIPKDTILFWKKELAQQNVFE
                     YLEKIICRYGLLGTYDEINQDGWKIIVIRHRLGKKGSVFFHGYIKSILNIVGLDNNIE
                     LTESSVKAKIVM"
     gene            complement(4662..5093)
                     /locus_tag="VF222_13945"
     CDS             complement(4662..5093)
                     /locus_tag="VF222_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143575.1"
                     /translation="MRTKRDIKKIDKHKVMIIEDEIDILLLYKDYLNNKGFSVQATST
                     TANEALKDYENFYPNMIIFDYKLPGKLTGLEAAKQILAKYPRVPIIIVTAYETVKSEL
                     SNDNFFTDKNIILLLKPIKLHVLENTIKNLLSKKSNHTSYV"
     gene            complement(5902..6348)
                     /locus_tag="VF222_13950"
     CDS             complement(5902..6348)
                     /locus_tag="VF222_13950"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7143576.1"
                     /translation="MSVNKSFAEDNKYSTPLTGMEEVPPVNTNSTGIALFEIKNNNLG
                     FNVNVTNLSNITAAHIHLGEFGQNGEILVPLLKASQPFDILNGTLIEGNVTNTDLVGS
                     LKGKTINDLVQLFNNTKTYVNVHTEQYPNGEIRGEITTVNATDWWT"
     gene            complement(6595..6963)
                     /locus_tag="VF222_13955"
     CDS             complement(6595..6963)
                     /locus_tag="VF222_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143577.1"
                     /translation="MDFDFLEKLSENWKKSKDLRIEKKIINLICTTNKNHFAFPIAEK
                     CLISSGRVGFTYKDNKTYPNFFEFLSHITSRQIPIEIKNCHFGPGEIVVLRDNKGDAL
                     GDLILSTHQIVNEIKTKFTK"
     gene            8032..8556
                     /locus_tag="VF222_13960"
     CDS             8032..8556
                     /locus_tag="VF222_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX7143578.1"
                     /translation="MTLIGNLFRWVHGKVSNKPTNFSWVIDNKLAGSGTPMTLEQYRW
                     LIKNNIKSIVTLKEFPLPERWLVENGKDTIIKEDYKFVYVKDYGVPTLEVLDSIVDFI
                     DYKITHENKPLVVHCAAGKGRTGTILAAYLLKQGSISSPDAIKKIRMIRPGSIQSKVQ
                     EETLHEYEKFLKVL"
     gene            9089..9466
                     /locus_tag="VF222_13965"
     CDS             9089..9466
                     /locus_tag="VF222_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143579.1"
                     /translation="MEGNQLIKYNKCNIETLLQKALSAALMDLDPLKNSYYNRHLILE
                     IEILQWMLDIIKKFGYNLTFLKELIKHEIDNFKVRYNSVKDIYEIDKVSNSIEILQTC
                     LFLINIEYEFQHLHPLMAKEKII"
BASE COUNT         3492 a         1299 c         1359 g         3447 t
ORIGIN      
        1 tgagggatgg ttttaataga ggttatgatg ttattcttat ttctgatgct acagcctcaa
       61 caaattttag gcactacaaa actaccatag aacacgttaa agagtattac ggattagtaa
      121 tgaaggctaa agaaatggaa agactaatgg aagcaaataa gagtggtggt tatttagaaa
      181 aaattaagat gattagagaa aaaagaaatt tactagagcc cgatcataat tgattttaat
      241 gaaataattt tggataataa aattatattt cccattattc taagaattga tcaataaata
      301 aagcatatga aatgtctatt aaatagagtt ttatttttgt tctatcagat aattataatt
      361 gattataacc tttcgtttag actaagtaca ctaaactaaa aaaagcttga ttggtgattg
      421 tatttctaac caaaaaaatt agtgttgatg gccacagcca caacctgccg tatttcctgc
      481 tccttgactt tctttttcta aacatgatgg acattttgcc ataccagttt ccgacatgaa
      541 tgtgctacca catgaactac attttaattt ttgaattcct tccatgaaat aacttgttat
      601 tatccttatt aaaactttga ccatttaaga gagtattgat atctttgaca aacatcatga
      661 tttattttac taaatatttt tttctattgt aaatcttcac taaaaattaa gaataataca
      721 taaagaaata taaggaaaaa gacactaaat gatactaatt tattacaatt gaattaccat
      781 gagataatca ccacatcgaa ttctcgaaga aaaatatgta attaactaac tatttatata
      841 tggaaaaaaa ataattttta aattttagac tttgtaaaat tggaataaaa tcggttaaga
      901 ttagttaaat aaaaaaatgt ttaatacact tatcctacaa taaatgagat agattgtcta
      961 attattagat ttaagtagtc caaagacatt aaacaataac aaataattta aataaatata
     1021 acagtatatc atatatttga atattaataa aaaaggtatt cgagttttag ctattgcgga
     1081 gagctttaaa aaagaaaaat caaattctat tttaacaggt gttgtaatga gaaaagatct
     1141 aatcattgat ggtataatct ttggaaaagt aacaatagga ggcaacgacg cgactcaaaa
     1201 tattctcgct atgtataata atttacagag gaatgatatt ggctttattt tatttgacgg
     1261 catgattata agtatgttta atatagttga tggggaaaca atacacaata agacgggtct
     1321 tcctgtgatt ataattacct ttaagaattc tgaagggata cataatagta ttaaaaaata
     1381 ttttccagac acttataaaa ctaaaatcac tctcattgaa aaactcggca aaagagaacc
     1441 agtattacta aaaactaata aaattctttt gataagaacg tggggtatta aattggacga
     1501 tgcagttaga cttttaaatt cctttttaat tcagggctct attccagaac caattaaaat
     1561 agcaaaaatt atatcacgtg cttatttaaa tactctttaa aaatttacta aattagaaaa
     1621 ataagtttga agtctaataa ccctatatac cacaaaagcg tactataaac caacaaaata
     1681 aaggtctagg tacaacaaca aagcttacta taacaattat ataatttatt gtactttgta
     1741 aaatatgaat gaacaaactg gtttagacaa tactacatat gatatcctat ccgccctagg
     1801 gagagaagca gattttttat atgataccat tgataaatat attgacgatg ctaaacagtc
     1861 aaataatgat gaattagtag acttatggaa caggatcaag caagacagat taaaacatat
     1921 caatgatctt aaagaggcat tagaacgagt catccataag taaattttat aaagctattt
     1981 tttttgtttt attagtttga taattataaa atgtccacta tctgatatct catctttcta
     2041 cttctttttc atttattgat ggatcattaa aattttattt gaaaggagca ctcatggaga
     2101 tttaaattag atagaaggag tataaattat cttaataacc ttttaggttt atatttttct
     2161 aattacttta ctaagattaa agggaaggaa attatacttt tacagaaata tgaatatata
     2221 attttcttca aatagagtgt tttattcaaa aaagaaaaaa ttagaataga gatgaaaaaa
     2281 attatgttat gtttttcgtt ttcttagttc tattgtattg tttggtatat gaggaaaaat
     2341 tatttttaca tctaaaaatc gttttcttaa tgtcaccaca ctcatttctc ctgcaatagc
     2401 aacttgagat tgacttatgt tttctccctg ttcgagacag gcagcatata gtactgctat
     2461 ggacaatgca gttggatcct tgccagaaga aaagggatgt tcttttattt ctgccatcat
     2521 ttctattgct ctatgtagtg tttttttact tatacctccc ttatttgcaa ttttacttaa
     2581 atatgtagta gaatctatgg gttgaagcct gattttcaaa tttcgtaata gcaatctata
     2641 gcattttcct gcaaaaactt tatctgcatt cgcaatctct gcaatttcgt ctaaggtgcg
     2701 aggaatattc atttcccgac aggctacata tacacaagcc acaacgaact cttttataga
     2761 acgacctttt gtaattcgct tctctaccac tttacgataa taataagctg atttttctat
     2821 tatggtgtca cttaaagaaa gtttatcctt tatccttagt aatacaccaa atgcattttt
     2881 tagatttcta tgataactgc ggttattgct agatatctta ttccaatgac ggattgttct
     2941 tacttttgcc atttggctcg tatttaattg actcccaaac gcgtctacat tagatgaaga
     3001 aatcgttgtt gaaagtcctt tatcataatg agctaatgat aaaggcattc ctacacttaa
     3061 gttattttca atatttttca taggttcaga ttcaatacca gacgaatcca atcggtcttc
     3121 tactatcatc ccgcaagaag tacagatcat ttctccattg gatgaatcaa atataatatg
     3181 cggattgcga caattaggac aatcatcagc aattttttca ctgataccgg tatgtaataa
     3241 ttcaggcgtt gtagtcatat cccttgtgtt aacgtaagtc ttataagcac tatgtttaca
     3301 aattaacagg tttaagaata ttaatacaaa ataacactaa ataacacgtc ataacacaat
     3361 tatagatcag tttcttattc tatacaatgc ctaattcaac tcgttcgttt cgagtagaac
     3421 aatacatatc caacatctta gatgaagaag cggaacggat gggcatttcg gtaaatgcac
     3481 tcgttaatat tatcctcaaa cactattctg attttagtcg ttttttatcg aaaatcgact
     3541 tagtgatact aaatagagaa attttaacaa aatttttaga aaatatggat gaccaggata
     3601 tttttaaaat tgggttagaa ttaggagaaa caattcctaa agacacaatt ttattttgga
     3661 aaaaagagtt agcccaacaa aatgtttttg aatatttaga aaaaattatt tgcagatatg
     3721 gattattggg tacatacgat gaaataaacc aagatggatg gaaaataatc gtaattaggc
     3781 atcgattagg aaaaaaaggg tctgtatttt ttcatggtta tataaaatct attcttaata
     3841 ttgtaggttt agataacaat atagaattaa cggagtcatc ggtaaaagca aaaatagtta
     3901 tgtaaaaatt ataaaagtca tggacaaaaa aatgataatt aaacatccct ttttagtgag
     3961 tgtatttatt tagtagacat aaattttcag aacctgatga atggatatct tgagcgtaaa
     4021 tttttataaa tctatcaaag ttcgtcaata taataaagaa taacgagcgt tttatcctta
     4081 ttcttataat ttaatagtta ttttgaaaaa ttgtatttat aaaattaatt atattataaa
     4141 aaattttctt attcacttat taatttatta taaatgatat tcaaagttaa tttcttattt
     4201 tttaatattg tttgaattta aaggtttaag ttgtcgaatt ttcaagtgaa atgtaaattt
     4261 tatatataag catttgtata ttacctagta atatcagaaa ctagtgaatc ttttccagtt
     4321 accattgtcg attaattcat taaaaaacac gatatttttt acattactat gtaaaacttc
     4381 ttcattctga atagaataaa tgactagatc attgatcctg tgagcaataa gggttaaatc
     4441 gtttttaaaa tctatatata tttctaaatt tacggcaacg tcatggttat catcattttt
     4501 atttctatcc atatatcaaa ttatgaccta ttagtatata aaatcaaatt acatctttaa
     4561 tacagcatac aaagagtaga ttaatttcat acagctatta acaaactcta aagtttctct
     4621 cataattatc ttatcttcaa gtagattttt taatacggca attaaacata ggatgtgtga
     4681 ttgctctttt tgcttagaag gtttttgatt gtattttcca gtacatgtaa cttgataggt
     4741 ttaagtaaca ggataatgtt cttatcggta aagaagttat cattcgacaa ttcactttta
     4801 acagtttcat atgctgttac tattattatc ggaaccctgg gatattttgc gagtatttgc
     4861 ttggctgctt ctaagccggt tagttttcct ggaagtttat agtcaaatat aatcatatta
     4921 ggataaaagt tctcgtaatc ttttaaggct tcattagcag tagtggatgt agcttgcaca
     4981 gaaaatcctt tattattgag atagtctttg tatagaagaa ggatatcaat ttcatcttct
     5041 attatcatta ctttgtgctt gtctattttt tttatatctc tctttgttct catcacattt
     5101 tactatatta atattatttt catcttaaca aataaatctt ataacttttt tacaactagt
     5161 tatattggtc atggtttatt ttttctctta gaaattatat tgtaacttaa tatagattaa
     5221 ttaaatctgg tctgttgtta tcttatattt atcatgacgt gacttttgaa ataattctgt
     5281 ttataaaaat tctgggtttt gaatattgtt ttaggtacga cttagttaaa aattagttga
     5341 ttataatgca aatagtaaaa atccagaaag cctattgaag tgctattaaa tatgttgtga
     5401 aagtgtttat aatgatggat atacacctca taggattaga agttgttgat cctgatccct
     5461 ctcctaaccc agaaccggag cctgaaccac aaccttcacc tgaaccacaa aacaaataaa
     5521 tacttcttca aataggctaa gatgacttac caattctaaa tatttttttc tattttgagt
     5581 gtctaataga cacactttat aattactggt taaacaattc tatttactta aagtgtttta
     5641 ttataataaa caatatatat attgacaatt tcaaagtaat attttctaaa ttataatgat
     5701 gacgtggttt tctataattt atagttatat tttcatgcgt acaccagcat tcacctttat
     5761 aaggagaagg gattaatatc taaaagtttt aaagatttaa ggttacaatt taagatatga
     5821 ttaaaatatc aaatatggaa atttaaactt taattttgtc caatatcaga agaggaaaag
     5881 agtattgaat gaagtttagt attaagtcca ccaatcagtc gcattaacag ttgttatctc
     5941 acccctaatt tctccatttg gatattgctc agtgtgtaca ttaacataag ttttcgtatt
     6001 attaaataac tgaacgaggt cattgatagt ttttcctttg agagaaccta cgaggtctgt
     6061 attggttaca ttcccttcta ttaatgttcc attgagaata tcaaatggtt gtgaagcttt
     6121 caatagggga actaggatct ctccgttttg tccaaattca cctaaatgga tatgagcagc
     6181 tgttatatta ctcaagtttg taacatttac attaaaacct aaattattat tttttatttc
     6241 aaataatgct atgccagtac tatttgtatt aacaggagga acttcttcca tccctgttaa
     6301 aggagtagaa tatttattat cttctgcgaa agatttgttg actgacaaga aagatataat
     6361 tattaataaa agtgatagta ttaaaatagg actacttttc atcatttgtg catgttaaac
     6421 atattatata atcgttacag gtaaaaattt tatagaaaga ttgttatcta taaataattt
     6481 ttaattgttc taatttgtgg tagtataata taatttaaca ataataaaat tatgatgtag
     6541 atttaattca tcgttaatta ctaattgata atgagataaa aaaggtaaaa taacttattt
     6601 agtaaatttg gtttttattt cattaacaat ttggtgagtt gatagaatta aatcacccaa
     6661 ggcatcccct ttgttatctc gaaggactac aatttcccct ggtccaaaat gacaattttt
     6721 tatttcaatt ggtatctggc gactagtgat atgcgaaagg aactcaaaaa aattaggata
     6781 agttttgttg tctttatagg taaaccctac ccttccacta ctaattagac atttttctgc
     6841 tattggaaat gcaaaatgat tcttgttagt agtacaaatt agattaatta tttttttttc
     6901 gattcgcaaa tctttagact ttttccaatt ttctgaaagt ttttctaaga agtcaaaatc
     6961 catatagtaa ttgatatgta ttccattatt aaaattgttt ttgagattta taaaataatt
     7021 atatgcatta atcataccta gtatatatat atttcttatt ttataaaagt attaaaaaat
     7081 tagagaaaaa aaataatagt attgtccttg gaattattct atttttataa atatagtaat
     7141 tattagataa aactcaatta atatattttt aatctactaa tattcttttc gacattttga
     7201 taaaaatatg ataatatcaa atattttcag ttttgacatt agtaatcttt actgatcact
     7261 tttttaaaac caacctggat acattaataa taataaatcc caaagcctct agtggaaaac
     7321 atgaaaatga tttagagtat tttgtaatac attaacaaat catctgggcg ataaattaat
     7381 attaaaatca caaagccgtg atactccaag atttgctaga aaatatcttg aaaaattttt
     7441 agaaaattat agtaattgat ggagatggga ctattaatga agtagtgaat ggtttttttg
     7501 aaccttcacc taaatatatt accaattctt aggaaagcca tgatttaatc ccaatttcgt
     7561 ccacgagcag tattcttcag cgtgccagtc gttacaggaa tattttagca taatcgctca
     7621 acattcccat ccggaggatt tcagcctcca tataatgcaa aaaaaaagat ggttactaga
     7681 tttagttata ctagatttag ttatagttaa ggattctgga tgtttaagat ttttgactgg
     7741 tttaatggat acaaaaatgg aagaccattc tgacaatccc aatataatat atattaacta
     7801 cctcgttgga cggagaagtc acaataacaa tcgatggaga acccatatga attcttcctg
     7861 ctcgtttcga atccataaat aaattttaaa tattatgaca taattactat tgcagactca
     7921 ctcaaaaact attttcaaat tgtaattaaa ataaaatcac aggaacacta tattggttgg
     7981 atgaagtttt atctccttaa aatataaata tttaactttc catgttaagt gatgacacta
     8041 ataggcaatt tatttaggtg ggttcatggt aaagttagta ataaacctac aaatttttct
     8101 tgggtaattg ataataaact agcaggtagt gggactccaa tgaccttaga acaatataga
     8161 tggctaataa agaataacat taagtcgatt gtgaccctaa aagaatttcc tctaccagaa
     8221 agatggctcg ttgagaatgg aaaagatact attatcaaag aagattacaa gtttgtgtat
     8281 gtaaaagatt atggagtgcc tactctagaa gtattggata gcattgttga tttcatagat
     8341 tataaaatca ctcatgagaa taaaccatta gtagttcatt gcgcagcagg caaaggaaga
     8401 actggtacaa ttttagccgc gtacctgcta aaacaaggaa gtatatcttc tcctgatgca
     8461 ataaagaaaa taaggatgat tcgacctggt tcgattcaat ctaaggtaca agaagagact
     8521 ctacatgaat atgaaaaatt cttaaaagtt ctataggatc atgaaagata ttgatttacc
     8581 taaataagcg tgggtctatt tttctcgaca tccctttaac attttgcatt ttttatgtgt
     8641 atttattata ataatagaca aacaaatata taaatattat agctactcat gtaattttta
     8701 tgcaacaagt tgaaacaaat atctaaaaat agcaatacac ttcaatggat tctcctgaaa
     8761 aaatcattta tttcttttgg aatcgagaat atcatcttaa cactaataaa ggtaaagagt
     8821 ataagctctg gtctataggc ttcagattcc aattaggtca tgtcatagga tatataaatt
     8881 agcattaaga tcatagaggg cactaactct taagagcctg ctaggcgaaa tagtattttt
     8941 ttattaaaaa gtttagcatc ggcttaagtc atgttatcca attaagtttg gacaactctc
     9001 ccacacattg gttttgtata aaatttcccc ccttccctct ctggaaggtg ctactaatct
     9061 aaagtagtca ttaaaatagt atttttttgt ggaaggaaat caattaatta aatataataa
     9121 atgtaatata gaaacgcttc tacaaaaagc actctcagct gccctaatgg atttagatcc
     9181 attaaaaaat agttattata atagacattt aatcttagag atcgagattt tacaatggat
     9241 gttagatatc ataaaaaagt ttggttataa tctaactttt cttaaagaat taattaaaca
     9301 tgaaatcgat aactttaaag taagatataa ttctgtgaag gacatatatg aaattgataa
     9361 agtatcaaat tcaattgaaa ttctacaaac atgtttattc ttaatcaata ttgaatatga
     9421 atttcaacat ttacatccat taatggcaaa agaaaaaata atataaacat ccaaatgtaa
     9481 aaatgaatta tctcctactt gttgaatatg ctaaatagtt tttcattccg attgtcatca
     9541 gacttaatca agaagagaac ttggattttt ctattaaata aatataaata taataat
//
LOCUS       DASVEV010000322        12347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1737542, whole genome shotgun sequence.
ACCESSION   DASVEV010000322 DASVEV010000000
VERSION     DASVEV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737542"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="VF222_13970"
     CDS             complement(<1..2076)
                     /locus_tag="VF222_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7143580.1"
                     /translation="MSDIREPDSPIHPHQHKLVRSLGLLDVVMIGIAGMIGGSIFVLT
                     GPAIGLAGSSVILAFILNAIITLFTAMGYAELGSAMPEAGGGYLWVREGLRRPNAFIS
                     GWMAWLAHIIAGSLYSVGFGSFLVSLLQMTNIITIESLFGIIPLDKLTAVAVVAVFTF
                     INIKGASETGRVGTIITLAQLVAIVSIIIAGLWAMSSHPNDWQSNFSDFLPMGLGGLV
                     AAMGLTFIAFEGYEIIVQTGEEVKNPKRNIPRAIFISLALVVIMYCIIAFVSIGAIFP
                     DMSSWQFIGLHGELGIMKAAELFMPYGAFIVLAAGLVSTLAALNATTFSSARVAFAMG
                     RHYNLPHKLSAIHPKFKTPYVSVVLSCIIMAVMAYALPLEDIAHAAGVIFLLLFTQVN
                     LAVISIRRMYGDKLDYGYKTPFFPYVPIIGIILMISLAVYLLITVPLSWAITIFWVLI
                     GFTIYRIFTFKKEVEHYSPTITSEGNLERKDFRILLPYTPENPDRLIRYAIEVAKEKD
                     GEINILRTIRVPPQTPLSAGIAFIDSARKTFDSLEEILNKEDVIYHYYVRISHDATEA
                     VLTTIAEQKIDLMIIDYETMRSNKKLQTLLTCDVLAIIPHSEDYIILERQNNTDESGV
                     TKDDKRNMVVLYDDGDNSDEILKVTTWFANNERLNLNVVAINRRNLNKFHYKTKESIK
                     GKNNDKIAST"
     gene            complement(3501..3647)
                     /locus_tag="VF222_13975"
     CDS             complement(3501..3647)
                     /locus_tag="VF222_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143581.1"
                     /translation="MSSMIYGYETAPATDLVKYIFYIDSIDSIVVKWGQTQTPTFMDL
                     WMIK"
     gene            3898..4554
                     /locus_tag="VF222_13980"
     CDS             3898..4554
                     /locus_tag="VF222_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143582.1"
                     /translation="MWQNNKYTVSIIIIVIATTFVSSLALYQNAYAHQRQLFTIGNDE
                     YLFVVGSLNEPIFVDDKTGVDMVAYWPDPNDPVNSKANGTKPIVGLENMLKVELAAGD
                     KKKILNFEPAYQNPGHYEASFFPTIETTYNYTLFGNINGTEFRATWTCSPAEGEPMSD
                     NLTKQISDGVIRKALIGSFGCPQPRTDLGFPESYLANNEILKKLTELEKMVNPPNKTM
                     "
     gene            4842..6203
                     /locus_tag="VF222_13985"
     CDS             4842..6203
                     /locus_tag="VF222_13985"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7143583.1"
                     /translation="MISSFNTVVQTSYGQLTIESKSETAITSYTDAIIKAPLLIAQAL
                     IVGITFVHSSIFIPIIKKDSIFFNNNDENFIWPFLANNKQFIIIISACGAIIFNMSTI
                     LFVYQASLLSSDLGLDLSSTLSLLLSSSVGNIYIIKIVTSTIIIILSILYYYIAKTKG
                     RWKYKEKQNIKHQKIKTIKVSLTFCILILILDSINIFSNSIQSHSAAVSFFPYIAISV
                     DLLHFMMVSIWVGGLFYISLISRKLLIKVPNPQQYSISFINSTNMHLFLSIILRFSII
                     AIISIGTIFITGLYMGLLHLQQPSSLLNSSYGNTLIVKLLIVFPMALLGGYHQFRLHK
                     LINQKIWIQVNQQLQKLKIFHKSLKIEFILGIGVIFTSSFLTITSPPQHDEFHNMDMK
                     IWLPSEQSDLTKKITNSQNYLFDQSFSFIVLVLSISIAISVIFFTRRSWLNLKIYNQN
                     YHI"
     gene            complement(6323..7090)
                     /locus_tag="VF222_13990"
     CDS             complement(6323..7090)
                     /locus_tag="VF222_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX7143584.1"
                     /translation="MRIILITGIFSFFVVLGILNGSINVFGHANPVLYNPPSNSIFQS
                     DQLLPTNVTILYSERPETKVSYLHVLNSLSERVDNNDFKIIGQDERGALVTLDSTKLS
                     NGTYTVSWLVLSKDDGHITKGSYVFTITDTFETTNTTTKDVEANTFLERTVIDKVNLE
                     YEITPLQVGINTFTITLKDENNKPLSNINNVIMQLTNQNQNIGPIIANLEKTGDEAYR
                     ITGAYLSQEGEWNVKITVQRTGQYDLNHSFDISIASK"
     gene            7333..7485
                     /locus_tag="VF222_13995"
     CDS             7333..7485
                     /locus_tag="VF222_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143585.1"
                     /translation="MKFTIINIKQVQLLTITVVVVALLLFNSSNYKNQIYGHNFSGDE
                     SAIFWH"
     gene            7585..7782
                     /locus_tag="VF222_14000"
     CDS             7585..7782
                     /locus_tag="VF222_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143586.1"
                     /translation="MFNSTIKQNIPSNATENANAIESGLQQLKDMIKSKASYDKVMGI
                     IYGIIQTNTQAIFNLPLKTSK"
     gene            8196..8861
                     /locus_tag="VF222_14005"
     CDS             8196..8861
                     /locus_tag="VF222_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143587.1"
                     /translation="MRKIQLNFLSIFLIYIWTPIIPIHFIPLEDIKWINWIDLQYIFT
                     LQYPSLDYWKIIEKVNQNNSNLSYDLRDIDKNINDDSYDIAILHTDNSVTNVKIAFNA
                     MPLEDISNRNGFPLFKSYSSYPDLLVEHFETREKINSSSTFQVVEKHSDKYLIDENKA
                     SGILSRYSSSLGNSYGILTLYAIDKDNDLVLMYKYISDANVFMTYLPLAEKIMDSIKI
                     LNN"
     gene            complement(8976..10016)
                     /locus_tag="VF222_14010"
     CDS             complement(8976..10016)
                     /locus_tag="VF222_14010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX7143588.1"
                     /translation="MNSILASSISFNNAEYNNFTSICRISVIACVDNISSIQREEGDI
                     KKQTDLILEQVNSTKLKEWINILSSFHTRHTKSSHIESVTYWLKNEFEKICENKVFVL
                     NFTQRDENQTYHLKNIICTRPGSIASFQDNNYNNTIIIGAHYDSRAENINNTEARAPG
                     ADDNASGVSAILELASILSHLNLKLNLEFVLFSGEEQGKWGSNSYVKYLDDNNRTKNI
                     DLYINFDMIGYRPSNGSGQVILEYDVGNKYLQNDNYSKTIALFIKQIASNYTNLQAQL
                     SKLGNSDFIPFEAFGLTVIGIHDGGVTENPNYHKSSDTPNTLNIEYITSITKMTLATI
                     LELDKLSQYLKR"
     gene            complement(10296..11357)
                     /locus_tag="VF222_14015"
     CDS             complement(10296..11357)
                     /locus_tag="VF222_14015"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7143589.1"
                     /translation="MTPPQKENSKSIRNRNDNSKFNDLIFILNPNSQGGNTGKNWDET
                     YSKIKEFLPINHQIIFTKKANEGTLITRKFLKEGYKNIVAVGGDGTINEVANGFFYNR
                     SSRNNSVTRSSGFKPTSHLKSVNPDAVLHIIPSGSRNVLAASLRLQYEGIDSFMHINE
                     MKKSKIDVIGVTTADKDDPTLTRNRIVLNAAEIGVGAEIINRSKKVRAKVKSRLLSTV
                     ASIISTVPTYESNSCEIILDNDKTISSNLTMGIIANGQFLGGKFNAAPKANMSDGLMD
                     VVILRNSGSIKMLDKLVSLKGDDSYTEEDNILYYQVSRVTLLPKERNMTVSLDGEPIG
                     ILPASFKVYHNALSLKVNS"
     gene            complement(12173..>12347)
                     /locus_tag="VF222_14020"
     CDS             complement(12173..>12347)
                     /locus_tag="VF222_14020"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7143590.1"
                     /translation="LSYNQLKEYLSILIENNLIEYLDGTHQFKTTEKGLNLLKMHNEM
                     AELLQQTTIIKDN"
BASE COUNT         4313 a         1794 c         1682 g         4558 t
ORIGIN      
        1 tgtagatgca attttgtcat tattcttacc ttttatcgat tcttttgttt tatagtggaa
       61 cttattcaga tttcttctat ttattgcgac tacgttcaaa ttgagtcttt cattatttgc
      121 aaaccatgtt gtgactttta atatttcatc agaattatcc ccatcatcgt atagaactac
      181 catatttctt ttatcatctt tggtcactcc actttcatcg gtattgtttt gtctttccaa
      241 aattatataa tcttcactat gaggaataat tgccaaaaca tcgcatgtaa gcaatgtctg
      301 taattttttg ttacttctca tagtttcata atctataatc attaaatcaa ttttttgttc
      361 tgctattgtt gttagaactg cttcagtagc atcatgagaa attctcacat aataatgata
      421 tataacatct tctttattta atatttcctc aagagagtca aatgtctttc ttgctgaatc
      481 tataaaggca attcctgcag atagaggtgt ttgaggagga acccttattg ttcttaaaat
      541 attgatttca ccatcttttt cttttgcaac ctctatggca tatcttatta gtctatctgg
      601 attttctgga gtgtaaggga gaagaattct aaagtctttt ctttctaggt taccttcact
      661 tgttattgtc ggagaataat gttctacttc ttttttaaac gtaaatatac gataaatagt
      721 gaagcctatc aagacccaaa atatagtgat agcccaacta agtggtacag taattaaaag
      781 ataaacagcc aagcttatca ttaatattat tcctattatt ggtacatatg gaaagaatgg
      841 tgttttataa ccataatcaa gcttatcgcc atacattctt cgtatactaa taacggcaag
      901 attgacttgt gtgaataaaa gtaaaaatat gactccagca gcatgagcta tatcttcaag
      961 aggaagagca taagccatta ctgccataat aatacatgat aatacaacgg aaacataggg
     1021 tgttttaaat ttaggatgaa tagcacttaa cttgtgaggt agattgtaat gtctacccat
     1081 tgcaaaagca actcttgctg aagaaaaagt tgttgcattt aatgcagcaa gagttgatac
     1141 tagccctgct gctaaaacta tgaatgctcc atatggcata aataattctg ctgctttcat
     1201 tatacctaac tcaccgtgta gaccgatgaa ttgccatgaa gacatgtctg gaaaaatggc
     1261 accaatagaa acaaaagcaa taatacaata catgattaca actaatgcaa gcgagatgaa
     1321 aattgctctt ggaatattcc ttttaggatt tttaacttct tctcctgttt gaactattat
     1381 ctcatatcct tcaaatgcaa taaaagttaa acccattgca gctacaaggc cacccagacc
     1441 cataggcaaa aagtcagaaa aatttgattg ccagtcatta ggatgagaag acatagccca
     1501 caatcctgca attataattg agacaattgc tactagctgg gctagtgtta ttatagtacc
     1561 aactcgtcct gtctctgaag ctcccttgat gttgataaaa gtaaatactg ctactacagc
     1621 gacagcagtt aacttgtcga ggggaattat gccaaaaaga ctttcgatag taataatatt
     1681 tgtcatctgc aatagactaa caagaaatga gccaaaccca actgaataca aacttcctgc
     1741 aataatatga gcaagccatg ccatccatcc actaataaaa gcattaggac gacgtaatcc
     1801 ttctcgtacc cataaatagc ctccaccagc ttctggcatt gctgaaccta actcagcgta
     1861 gcccatagcc gtaaaaagag taataattgc attaagaata aaagcgagaa ttacagagct
     1921 tcctgcaagt ccgattgccg gtcctgtaag gacgaatatt gaaccgccaa tcatacctgc
     1981 tattcctatc attaccacat caagcaaacc caatgaacga acgagcttat gctgatgagg
     2041 atggatagga ctatctggct ctctgatatc agacatctat tgggtttcta gtaatatcca
     2101 ctatcaaata taagtatacg gagacaaaac tgtaatattt ttagaggact cgcttagtgg
     2161 gctaatataa tagtagaata ttttctaaaa ataaatcttg aaaatataag tataatgaag
     2221 atagaaaatt tattatggaa aaaaaagaaa aagaagaaaa taaacaaata tcaataattt
     2281 ttgcaaaaga tataatagaa atttaaaaaa tttcatttaa tgtaaatatc gggttccatc
     2341 tatctatttc aataattcat ctggacttga atgaacacga tttccatgtt caatatcaca
     2401 aatcgaatta actattaaag gatacatcta attttgataa tatatcctac ttcattttgc
     2461 gataatatat aatcgacctg tcggcttgcg aggcattttt gtctctattt gtatctaaaa
     2521 acgacagatt tttccaacaa ccacattctt tcgaataaag taatataaac aagtttcctc
     2581 gattcactat ttacaggttg gataaaatac cgattgtcat catctgtatt ttgtgattca
     2641 cccaagatat acatgcattc actatctaga atcaaagtaa ttgtatttga gctcagatga
     2701 cgatcgaaat atttaatcga gatatatgaa ttaatgtttg atgggatatc ctcttcattg
     2761 aatttagaag aaggtaagag tatgcgtata tttaataatt tatttttgtc acatttctga
     2821 atataaggag ttctatagtt ttttgttata attggtaaaa tccagtttaa ttctgttttc
     2881 tagatagata tataaaaata ttataaggca aatcatataa aaaatagata tctaaaattc
     2941 tggacattaa gttttctaat attatttttt aaataatact tttagaggag taagaataaa
     3001 agtcctaatc gaattaatat gttaagacaa gtaacaatta aaattgtttt aagaatatat
     3061 atctttaggc tacatggtga gtttatttaa aattcttcaa atgtgtatct caataaggtg
     3121 ccaaattata tttgttctta tcacattaat tgattttaaa tataatacat aatactaata
     3181 gtaggattcg tataaggtgt atttagcttt tattattaga aacttaataa caaattattt
     3241 ttttaaatat atagaataaa ctaaattact gattttagtt ggtaagcaag atttaatgac
     3301 aaattttaaa atttctctag taacttaaat ctttttttag aaaaattgtt atcttatata
     3361 gattggtctg tttcttagtt tttcgttggt caaaagataa ggaaacatta ctttcaagga
     3421 gtgatatctt accaataact agtattataa taataaaata aaatatcatg attttaataa
     3481 tttttatttt ttgtcaagct tcattttatc atccataaat ccataaaagt tggtgtttgt
     3541 gtttgtcccc atttaactac tatggaatct attgaatcta tgtagaaaat gtatttgacc
     3601 aggtctgtgg ctggtgctgt ttcatatcca taaatcatag aggacaattt atttcagatg
     3661 tattaaaata tttaacataa gtaatgcttt gaataaatat catttttctc tccttggaat
     3721 attaatttcc ttgtataaaa ttagtaatct tagtctacaa gaatcaccat agttatctgg
     3781 aaatttttta ttttaatcat tatgtatgta gaattctgta agatagaaaa gagttaattt
     3841 agtttctatc gatagttgta ccatattttt tatatacata ttctttctac cataagtatg
     3901 tggcaaaata acaagtatac agtatcaata ataattatag taattgcgac aacttttgta
     3961 tcatcacttg cactttatca aaatgcttat gctcatcaaa gacaattatt tacaataggt
     4021 aatgatgaat atctatttgt tgtaggctct ttgaacgaac ctattttcgt cgatgataaa
     4081 actggagtag atatggttgc gtattggccc gatccaaatg atccagttaa ttctaaagca
     4141 aatggaacaa aacccatagt aggattagaa aatatgttaa aagtagagct agcagcaggt
     4201 gataagaaaa aaatacttaa tttcgaacca gcttatcaaa atcctggtca ctatgaagca
     4261 tctttctttc caactatcga aacaacatat aactacacat tatttggcaa cattaatggt
     4321 acagaattta gagctacttg gacttgtagt cctgcagaag gtgaaccaat gtctgataat
     4381 ttgactaaac aaatttctga tggagttatt agaaaagctc ttataggaag ctttggttgc
     4441 ccacaaccac gaacagattt agggtttcct gaatcatatt tagcaaataa tgaaatctta
     4501 aagaaattga ctgaattaga aaagatggta aatcctccga ataaaactat gtagaataat
     4561 tgctataaaa atatctataa aatcaatttt gtagtaagag attaaataaa tgatgaaatc
     4621 agcattctct aacttgattc atatttcact aaattattat ttctattgac aaaatctgat
     4681 aatatagtta taattaaaaa attaataagg taatattaaa tcaacataat cttgaacaat
     4741 aatagtaatt cagacagata aatcaaatga acaaagttta caaattaaaa agattatctt
     4801 actttttctt tatcttgact atatcaatta tatttctatc tttgatatcg tcttttaata
     4861 cagttgttca aacatcctac ggtcaactta ctattgaatc aaagtcagaa actgcaatta
     4921 cctcttatac agatgcaatt attaaagccc cgctattaat tgctcaagca ttaattgttg
     4981 gaatcacttt tgtacactct tctatattta ttccaatcat aaaaaaagat tctatatttt
     5041 ttaataataa tgatgagaat ttcatttggc ctttcttggc gaacaataaa caatttataa
     5101 ttataattag tgcatgtgga gctattatct ttaatatgtc aacaatttta tttgtatatc
     5161 aggcttcctt attatcatct gatcttgggc ttgatttgag ctcaactctt tcactattac
     5221 tttcatctag tgttggtaac atatatataa taaaaatagt aacatcaaca attattataa
     5281 tattatccat attatattac tatatagcta aaacaaaggg aagatggaaa tataaagaaa
     5341 aacaaaatat aaaacatcaa aaaataaaaa ctattaaggt ttccttaact ttttgtatat
     5401 taatattaat tttagattcc attaatattt tttcaaatag tatacaaagt cacagtgcag
     5461 cagtctcctt ttttccttat atagcaatat cggtcgactt gctacacttt atgatggttt
     5521 caatatgggt aggaggattg ttttatattt ctctcatatc aagaaaatta cttattaaag
     5581 tacctaatcc tcaacaatat tctatatctt ttataaattc tactaatatg catctttttc
     5641 tatcaattat tttacgtttt tctattattg ccataatatc aataggtact atatttataa
     5701 caggactcta tatgggcttg ttacatctgc aacaaccatc ttcactttta aacagctcat
     5761 acgggaatac tttgatagta aaattgctta tagtttttcc aatggcatta cttggaggtt
     5821 atcatcagtt tagattacat aaattaatta atcaaaaaat atggatacaa gtaaatcaac
     5881 aattacaaaa gttgaagata tttcataaat cattaaaaat tgaatttatt ttgggtattg
     5941 gtgtcatttt tacttcttca tttctaacta taacgtctcc tccacaacat gatgaatttc
     6001 ataatatgga tatgaaaata tggcttccgt ctgaacaatc agatttaaca aaaaaaataa
     6061 ctaatagtca aaattattta tttgatcaat ctttttcttt tattgtactt gtattgtcaa
     6121 tatcaattgc aatatcagtt attttcttta caagacggag ttggttaaat ttaaaaatat
     6181 ataatcaaaa ttaccacatt tgacttttta tattatatat ttctattaac aggtacttaa
     6241 tttaaatatg aataagtaga agtttatttt tattttgatt agtataatat tcttaattaa
     6301 ataacattta tttttttcaa gattatttag atgcaattga tatatcaaaa ctatgattca
     6361 aatcatactg tcctgttctt tggactgtta tctttacgtt ccattcgcct tcttgactta
     6421 gataagctcc agtgattcta taagcttcat caccagtctt ctctaaattt gcaattattg
     6481 gtccaatatt ctgattctga ttggttaatt gcattataac attgttgata ttgcttaatg
     6541 gtttgttatt ctcatctttc aatgttatgg taaaggtatt tatgccaacc tgtagtggtg
     6601 ttatttcata ttccaaattt actttgtcaa ttactgttcg ttcgagaaat gtatttgcct
     6661 caacatcttt agttgttgtg tttgtagttt caaacgtatc agttatagta aaaacatagg
     6721 aacctttagt tatatgacca tcatcttttg ataatacaag ccatgaaaca gtgtaagtac
     6781 cattggaaag ttttgtagaa tctaaggtaa ctagtgctcc tctttcatcc tgaccaatta
     6841 ttttaaaatc attattgtca actctttcgc taagggaatt caaaacatga aggtaactca
     6901 cttttgtttc tggtctttca gaatacagaa ttgttacgtt tgttggtaat aattgatctg
     6961 attgaaaaat gctatttgaa ggaggattat acaaaacagg atttgcatga ccaaagacat
     7021 tgatactacc attcaaaatt cccaatacta caaaaaatga aaaaattcct gtaattaata
     7081 taattctcat atgtatatgt actgctcaat tatagtttac aaatataata tataataatt
     7141 tggtcttgtg agcttgaaca tatttacaca gtatgtcaat aaataagttt tgttacaata
     7201 actacacaat tttcaaaaat ttttttcatc atagtatgaa aaatgactaa ataaaaataa
     7261 tttatttcta atcttcattt gagcattgta tcataagttt tatctatgat ttattagagt
     7321 aatttgttaa tcatgaaatt tactataatt aatattaaac aagttcagtt attaacgata
     7381 actgtagtag tagtagcgct tttattattt aacagtagca attataaaaa tcaaatatat
     7441 ggtcacaatt tctctggaga tgaaagtgct attttctggc attaatggac cagatacaaa
     7501 cggagatgga tttaattata ctaatttgat tgcggataag catcatatca aacagctaaa
     7561 gaattaacta acaatacaat agatttgttt aattctacca tcaagcaaaa tataccttcc
     7621 aatgctactg agaatgcaaa cgctatagaa tcaggtcttc aacaactcaa agatatgata
     7681 aaatccaagg cttcttatga taaagtaatg ggaattatct atggaataat acaaactaat
     7741 acgcaagcaa tctttaattt gccactaaag acatctaaat aaaagatgat ttaatctaat
     7801 acataaataa aatatatgcc atatcaaaag aaaataaggt caattaaaaa tttttttatt
     7861 tatattttat agatttttaa cctatattaa ttaaatattg ttcttttaat tggagctaaa
     7921 tttatattca ttaaaaaaat tgataattta ttattctttt ttatacatta gtcgaagaat
     7981 acttcacaca ctttattgat gttgctgacc ttttttattg tgtcattcta atacttgtga
     8041 tataggcatt aaatgttttg aaaagaatta tcttcgagcg cttcgattcg ttggtcaata
     8101 tattcgactc gactattttt atctcattga aaagtaaatt ttccttcata tagatataat
     8161 taacttatta aggaaaataa ataataatat taataatgag aaaaatacag ttaaactttc
     8221 tttctatttt tcttatatat atttggactc ctataattcc gattcatttt ataccattag
     8281 aagacattaa atggattaat tggattgatc ttcagtatat atttacatta caatatccct
     8341 ctttagatta ttggaaaata atagaaaaag taaatcaaaa taatagtaat ttatcatatg
     8401 atcttcgaga cattgataaa aatattaatg atgatagtta tgacattgcc atactacata
     8461 ctgacaatag tgttacaaat gtgaaaatag ctttcaatgc aatgcctctt gaggatatta
     8521 gtaatagaaa tggttttcca ttattcaaat cttattcttc ttatcctgat ttattagtag
     8581 aacattttga gactagagaa aaaatcaatt cttcttccac atttcaagtt gttgaaaaac
     8641 attcagacaa atatctaata gatgaaaata aagcatctgg aatattatcc agatattcgt
     8701 cttctttagg aaatagttat ggtatattaa ctttatatgc tattgacaag gataatgatc
     8761 ttgtattaat gtataagtat atctctgatg ccaatgtgtt tatgacatat ttaccattgg
     8821 cagaaaaaat tatggattca ataaaaatat taaataacta gtctaatttt ttctattttt
     8881 agaactaatt aaaaggtaat aataaaattt ataaattgta ttatacctaa tattgaacca
     8941 acataatttc ttatcttttg atagtaaaat attaattatc tttttaaata ttgacttaat
     9001 ttgtctagtt ctaaaatagt agcaagtgtc atttttgtta tagaagttat atattctata
     9061 ttcaatgtat tgggtgtatc agaagacttg tgataatttg gattctctgt tacacctcca
     9121 tcatgtattc ctataactgt aagcccaaat gcttcaaaag gaataaaatc actatttcca
     9181 agtttagata actgggcttg tagatttgta taattagaag ctatctgttt gatgaaaagg
     9241 gctatagttt ttgaataatt atcattttgt aaatatttat tgccaacatc atattctaat
     9301 atgacttgcc ctgatccatt agaaggtcta tagccaatca tatcaaaatt aatgtagagg
     9361 tctatatttt tggttctatt attatcatcc aagtatttta catagctatt tgatccccat
     9421 ttaccttgtt cttctcctga aaataataca aattcaagat taagttttaa attcaagtga
     9481 gatagaatac ttgcaagttc taaaattgca gaaactccac ttgcattatc atctgctcct
     9541 ggagccctag cttcagtatt attgatattt tctgctctac tatcataatg tgctccaata
     9601 ataatggtat tattataatt attatcttga aatgatgcta tcgaacctgg tctagtacaa
     9661 ataatatttt ttaaatgata tgtctgattt tcatctcttt gagtaaaatt gagaacaaat
     9721 actttatttt cacatatctt ttcgaattca tttttaagcc aataagtaac actctcaata
     9781 tgacttgatt tggtatgtct ggtatggaaa gatgacagaa tattaatcca ttctttgagt
     9841 ttagtcgaat taacttgttc tagtattaaa tctgtttgtt tttttatatc gccctcttct
     9901 ctctgtatag atgatatatt gtcaacacag gctattacag agattctgca tatggaagta
     9961 aaattattat attccgcgtt attgaatgat atagaacttg ctagaatact attcaatgaa
    10021 ataacaacaa ataaagcaat tattattgat attaataagg ataatatatt atatttttca
    10081 taggacaatt atcactttaa ttgcaatctt taatataatt gccttatcat ttgagattaa
    10141 tctcgcattg cagatatctc tatacattag tagagatggt aatcgtattg aggtatttga
    10201 taatgatggt aatttcatca agatttgggg aacgtactca ataacaaaat aaatcaagaa
    10261 taactatttt gattttcatt gttatttacc aaatcttatg aattgacttt taaagataaa
    10321 gcattatggt ataccttgaa tgaagctggg aggataccta ttggttcacc atctagactg
    10381 acagtcatat ttctttcttt tggaagtaaa gtgactcgtg atacttgata atagaggatg
    10441 ttatcttctt cagtataact atcgtcacct tttaaactga ccaatttatc aagcatctta
    10501 atacttcctg aattcctcaa gataacgacg tccattagtc catctgacat attagctttt
    10561 ggagctgcgt tgaattttcc acccaagaat tggccattag caattatgcc catagtaaga
    10621 ttagaagaga ttgtcttatc attatctaaa attatttcgc atgaattgct ctcatatgta
    10681 ggtacagtag aaattatact tgccacagta gatagaagac gactttttac tttagcccta
    10741 actttttttg atctattgat tatttcagct cctacaccta tttctgcagc atttaagact
    10801 atcctattac gagttaaagt aggatcatct ttgtcagctg ttgtaactcc tattacgtct
    10861 attttacttt ttttcatctc gtttatatgc ataaaggagt caatcccttc atattgtaat
    10921 cgtaaagacg ctgcaagtac atttcggctc ccagatggaa taatatgcaa gacagcatca
    10981 ggattaactg acttcaaatg agatgtaggt ttgaatccac tcgacctagt cacagaatta
    11041 tttctactag atcgattata aaaaaatccg ttcgcaactt cattaattgt accatctcct
    11101 cctaccgcta ctatattttt gtatccttct ttcaagaact ttcgtgtgat aagtgttcct
    11161 tcatttgctt tttttgtgaa aataatttga tgattaatag gaagaaattc ttttattttt
    11221 gaataagttt cgtcccagtt ttttccggta tttcctcctt gagagttagg attcaaaata
    11281 aagatcagat cgttgaattt agaattgtca tttctgtttc taatcgattt ggaattttct
    11341 ttttgggggg gagtcattgt tataaaacta cttccgtaga aaataactac aaagcgacta
    11401 aaagcactat tgtaatatta ttccttaaat tttagattga aaattcacaa agtgatttga
    11461 tagaataaat acaactataa ttaataatag aagaaaagat aacttttttt actgcaactg
    11521 tttagaataa attagaacct attcatgctg tttaaattat ctcttgtatt aaagacaagg
    11581 agaaatgatg caaaacttac tactaattag tttaccaatt ttttaacttg cagttttaat
    11641 ttataaacca tgttccttat gcatatggtt attgagagca ttctcatttt tagcaatata
    11701 attacatacc ctacaagttt ttttatcctt acgtccttgt aagtaatcgg caagccgcat
    11761 gagatacatt gttatacccc acatgtttat attaataaag aatcttattt aatgattata
    11821 tcttttcctt cgaaataaca aagacaagtt ctttaggaaa aaagaatata attgctaagg
    11881 gcaaagttaa tataaatgat ttcgaaagtt tcgatttcta aaaatgtaaa aaattaaata
    11941 gtaatagtaa ttagttttct atagcagacg aatataactt tcgacaagtt ctattatgaa
    12001 attattgatt ttttatttgt tatagtgaga gtcacatttt ttagcaatcg ggttcaccga
    12061 acctttaatt agattttata attagaaact tatttttaaa ataaatttaa tggagttact
    12121 ataaaaatta ggatttatgt atattttagt atatcaaaaa atattaaatt aattagttat
    12181 ctttgattat agtagtttgt tgtaataatt ctgccatttc gttatgcatt ttaagtaagt
    12241 ttaatccttt ttctgtagtc ttgaattggt gagtcccatc aagatattct attagattgt
    12301 tttctatcaa aatagaaagg tattccttta attggttata acttaga
//
LOCUS       DASVEV010000323         5614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1738360, whole genome shotgun sequence.
ACCESSION   DASVEV010000323 DASVEV010000000
VERSION     DASVEV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738360"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..965
                     /locus_tag="VF222_14025"
     CDS             240..965
                     /locus_tag="VF222_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7143591.1"
                     /translation="MTYNSVLKNTFEVIEAERGLQELQLFKHHWPIKSWDTYKMIKII
                     SGAKRESFVLDVGCYESPILPMLKRLGFFNLYGCDLVLKSDSSSKFNNMSSFVYHEDY
                     EPIAEMYNDKSYQLSIRNLEDTNYSDQMFDYVTSLSVIEHGINIEKYFREMSRIIKNN
                     GYLLTSTDYWPDKLVNNKNVLSKGTPDNIFSRDEIEYLVKIAEKNGFKLIEPIDFEYK
                     DKVVRWNSIDLDFTFIFFAMRKE"
     gene            1058..1279
                     /locus_tag="VF222_14030"
     CDS             1058..1279
                     /locus_tag="VF222_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143592.1"
                     /translation="MNTEAIKNFTLQIKNYGGNEIGESVSKAYIFTCRKRQKEFRIPI
                     KKSDEIEYTLSLHLMSCEYYSHKLEEAST"
     gene            1293..1544
                     /locus_tag="VF222_14035"
     CDS             1293..1544
                     /locus_tag="VF222_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143593.1"
                     /translation="MTVRAIEVWDAADRSESLYQRINDVLRDNNITREELIDIKYSTV
                     YIPEEKKITSQALIIFDKKEEIVMTELDVVDDKDELLRD"
     gene            1631..1816
                     /locus_tag="VF222_14040"
     CDS             1631..1816
                     /locus_tag="VF222_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143594.1"
                     /translation="MPISKYSIGDICPNLRNKNPISPSTNTATTNRATYYKRSYSSYS
                     SSQQSKPKMYNSFELHE"
     gene            complement(2281..3126)
                     /locus_tag="VF222_14045"
     CDS             complement(2281..3126)
                     /locus_tag="VF222_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7143595.1"
                     /translation="MSNNSQKRVVVVTGSSRGIGKAIAKEFAKNNYSILLNARDEKEL
                     IETVEEIRNEISDPNKVAYLTGDISEEKTCISLIEEAIKRFGRINVLVNNAGISGPSQ
                     RTNNITSRDWDYVIGVNLRGTFLCTREALKYMTTSSSNNSSISNNNINDVNRNNKSYD
                     YDSNFSIINISSVHESIPMPYSAPYAASKGGIEMFTKNIALEVAEKGIRINGIAPGAI
                     NTSMNKDIMEDPQKKKQEEEKIPMRRIGEPEEIAKVAFFLASTDASYITGTTVYVDGG
                     LTLSQ"
     gene            3551..3943
                     /locus_tag="VF222_14050"
     CDS             3551..3943
                     /locus_tag="VF222_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143596.1"
                     /translation="MNRTGLNLILQGMPSSLGAYHVLGSNIIVANRYILEYIKNTKSK
                     EEYNSFVFAVLAHEYLHSLGIIDENKVRKMTYKLCFDIFGKDHTTTKFAYNPLGAFPQ
                     LQMISNNNFETSFTQIKKFDDKNLSYIG"
     gene            4118..5362
                     /locus_tag="VF222_14055"
     CDS             4118..5362
                     /locus_tag="VF222_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7143597.1"
                     /translation="MYSKAIKGNQEIDPEELKQKIDNNENIFLLDVRTPQEYEAWRIS
                     YENHDNPKLIPVDRLFMKDPTLLKEIPKEKEIVTICAHGNRSMIAAKILNQLGYNVKS
                     VKGGMAGWNKVYDVAEIPVLKEAPFKIWQIRRISKGCIGYIISSKEDKTATVIDPSRE
                     IYESFLKTVQDNELQITQLLDTHQHADHVSGIVKLVKTITTETNMEKIRIYLSSLEEY
                     DTGKTELKDIMYVKDGDKIDIDDKVTLRAIHTPGHTNGSMSFIIEYGHNDNNNNNIND
                     KNPTTSYLFTGDTLFVDGVGRPDLRDQAKKFAELLYDTYHKKILQLPDDTVVLPAHFN
                     GTSIALKHTVPILETLGIIKKRVKLLSMNKEEFIDYIADTVQPRPGNYKTIISINKKM
                     LPYDEMEMADLEAGPNSCAISS"
BASE COUNT         2144 a          797 c          793 g         1880 t
ORIGIN      
        1 acgtttgtaa taatacttta gtcaattgta ggaaatccta tgcaaaatct gtgtttggtc
       61 tttggtctat caaattctag taagaattta gataatcaaa ctcgcattta ttagagataa
      121 accagaaact atactttttg aaaatacaac ccgtagacgg tagtcatcag atataatctc
      181 ttttatgcat ctacaacgat atttataaat cttagacgaa ccccacagat aacttagatt
      241 tgacctataa ttcagttttg aagaatacat ttgaggtaat agaggctgaa cgtggtcttc
      301 aggaacttca actgtttaag caccattggc caatcaaatc atgggataca tataaaatga
      361 ttaaaattat cagtggtgca aaaagagaat cttttgtact ggatgtagga tgttatgagt
      421 cacctatttt acctatgctt aaaagattag ggttttttaa cctttatggt tgcgacctgg
      481 tactaaaatc ggacagtagc tcaaaattta ataatatgag ttcattcgtg tatcacgaag
      541 attatgaacc aatagctgaa atgtacaatg acaaatcgta ccaactttca attcgaaatt
      601 tagaggatac aaactacagt gatcaaatgt ttgactatgt cacttcttta tcagttattg
      661 aacatggtat taacattgaa aagtatttta gagaaatgag tagaattata aaaaataatg
      721 gctatcttct gacatctaca gactactggc ctgataaatt agtaaacaac aaaaatgtcc
      781 tttctaaggg tacaccagat aatattttca gcagagatga aatagaatat ctggtaaaga
      841 tcgcagaaaa aaatgggttc aaactaattg aaccaataga ttttgaatat aaagataagg
      901 ttgttcgatg gaattcaata gatttggact ttactttcat cttctttgct atgagaaaag
      961 aatgaaactt ttctccagca aaaattctat ttagatcata caatatttga agcaaattaa
     1021 aattacagta gaacacactg atagagaaga ataagaaatg aatacagaag caatcaagaa
     1081 tttcacatta caaatcaaaa actatggagg aaatgaaatt ggcgaatctg tatcaaaagc
     1141 atacatattt acttgtagaa agcgtcaaaa agagtttcga atacctatta aaaaatctga
     1201 tgagattgaa tatactctct ctttacattt aatgagttgt gaatattatt cacataaatt
     1261 agaagaggcc tcaacctaaa ggagaataag aaatgacagt aagagcaatt gaagtttggg
     1321 atgctgcaga taggtctgaa agtctatatc aaagaatcaa tgacgtatta agagataata
     1381 atattactag agaagaactt attgatataa aatattctac tgtttatatc ccagaggaga
     1441 aaaaaataac cagtcaagca ttaataatat ttgataaaaa agaagaaatt gtcatgacag
     1501 aattagatgt agttgatgat aaagatgaat tacttagaga ttagtaagga aagagatctt
     1561 ctactaatat aatattaatc caaaaccgta cttatggttg taatactcac atatgttgga
     1621 atattgaaga gtgcctattt cgaagtattc aataggagac atttgtccta atctaagaaa
     1681 caagaatcct atatcgccat caacaaatac agcaacaaca aatagagcta cttattataa
     1741 gagatcatat tctagttatt cttcaagtca acaatcaaaa ccaaaaatgt ataattcatt
     1801 tgaattacac gaataaagtc tctattgctt ttacatcatg ttataataat ttggaacaat
     1861 tattatctta tattttaaga agacaagaaa atcccttaag gctcttttat aggttttttg
     1921 tatatattgt gattgtaaag tatataaaat tctgatatgg ttcattattc attatcatca
     1981 ttaattgttt gtattgcttt tccctcgtca ttaattccta ctatactctc cgttattaca
     2041 aatgataaat atttatagat aatatatggt gctgatagga ggaattattg gcattaatat
     2101 attatatttc ttctatggtc ttttcatttc tggtacaact ttaacttatt caatgaacat
     2161 aaaatgcata tactgttgtc tatatattag taaatgaaaa ataatacact ttaatttctt
     2221 atagtgtata gcaattgtta ttaatataaa aacaaaagtt atgattattt tactgtttat
     2281 ttattgtgac agtgtcaagc caccatcaac ataaactgtc gtacctgtaa tataacttgc
     2341 atccgttgaa gctaaaaaga aagctacttt ggcaatttct tcgggttctc ctattcttct
     2401 cataggaatt ttttcttctt cttgtttttt cttttgcgga tcttccataa tatctttatt
     2461 catagaagta tttattgcac ctggtgcaat accgtttatt cgtattcctt tttcagctac
     2521 ttctaatgct atattcttag taaacatttc tattcctcct tttgatgcag catagggagc
     2581 agaatatggc ataggtatcg attcatgtac ggatgaaata ttgattatag aaaaatttga
     2641 atcataatca taacttttat tattcctatt tacatcatta atattattat tactaatact
     2701 actattatta ctgctactag ttgtcatata cttcaatgct tctcttgtac aaagaaatgt
     2761 tcctcttaaa ttaactccta taacataatc ccaatctctg gatgttatat tatttgttct
     2821 ttgtgatggt ccacttatac cagcattatt aaccaaaaca ttaattcttc caaacctctt
     2881 tatagcttct tcaattaatg aaatgcaagt tttttcttct gaaatatcgc cagttaaata
     2941 tgcaacttta ttagggtcac taatttcatt tcttatttct tctacggttt ctatcagttc
     3001 tttttcatct cttgcattca acaaaataga ataattattt ttggcaaatt ccttggcaat
     3061 tgcttttcct attcctcgac tagatcctgt aacgactact actctttttt gagaattatt
     3121 agacatatgt aaatattatg ctacaagata ttttagagtt agataatcat cactaatcag
     3181 attttacttt atatggagta gtagtattaa ttcagtaata actaacagaa agccggaata
     3241 taataaaatt tgtagtatat atacagtata ttatttataa ctgattgata tctgttaaaa
     3301 taacattaag caaatgaatt aaaaaagaga aagtttatta tataatattt tgaatacacc
     3361 tatcttaata tctatttcat actcaataac aaaaatttat taaatcgaat gacaatatta
     3421 tatataggag atataaaaaa taaaccaaaa agttttagaa atagaaaaca atcaaaataa
     3481 gttaaatcaa ctagaagatt ttaaagagat atttgaattt ataaaaactt gtatatctac
     3541 tgtttttaaa atgaatagaa ccggattaaa cttaattcta caaggaatgc caagcagtct
     3601 aggagcatat catgttttag gttcaaatat catagttgct aatcgatata tattggaata
     3661 tataaaaaat acaaaatcga aagaggaata caattctttt gtttttgcag ttttggctca
     3721 tgaatactta cacagtttag gaataataga tgaaaataaa gtgagaaaaa tgacttataa
     3781 attatgtttt gatatatttg gaaaagatca tacaacaacc aaatttgcat ataatccact
     3841 aggcgcattt ccacaattac aaatgatatc taataataat tttgaaacaa gttttactca
     3901 aatcaaaaaa tttgatgaca aaaatctttc atatatagga taggaaatgt agtaatctgt
     3961 aatcctgatt tactatattc cactcatatc tttttatttg aatagtgtat tagttaattg
     4021 atgaatgaaa ttctgtagaa tgtattaaat tgtaataatt aatattttat ttaattaatt
     4081 taaacctaca aatatgatta gttatatatg ttagaatatg tatagcaaag ccataaaagg
     4141 gaatcaagaa atagatcctg aggaattaaa acagaaaata gataataatg aaaatatttt
     4201 tcttttagat gttcgaaccc ctcaagagta tgaggcatgg agaatatcat atgaaaatca
     4261 tgataatcct aaattaattc ctgttgatcg tttattcatg aaagatccta ctttgttaaa
     4321 agaaattcct aaagaaaagg aaattgttac aatatgtgcc cacggaaata gatcaatgat
     4381 tgctgcaaag atactaaatc aattaggata taatgtaaaa agtgtaaaag gaggaatggc
     4441 tggatggaat aaagtctacg atgttgcaga aattcctgtt ctgaaagaag ctccatttaa
     4501 aatatggcaa attagaagga tatcaaaagg atgcattggt tatattatat cgtcaaaaga
     4561 agataaaaca gcaacagtta tagatccaag tcgtgaaatt tatgaatctt ttttaaaaac
     4621 cgtacaagat aatgaactgc aaataacaca attacttgat actcatcaac atgcagacca
     4681 tgtatctgga attgtaaaac tagtcaaaac tataacaaca gagacaaata tggaaaaaat
     4741 acgtatatat ttgagctctc ttgaagagta tgatactggt aaaacagaac ttaaagatat
     4801 aatgtatgtc aaagacggcg ataaaataga tatagatgat aaagttacac tgagagctat
     4861 tcatacacca ggacatacga atggtagtat gtcttttatt attgagtatg gtcataatga
     4921 taataataac aataacataa atgacaaaaa tcctacaact tcttatttat ttactggaga
     4981 tacattattt gttgatggtg taggaagacc agatttaaga gaccaagcca aaaaatttgc
     5041 tgaattgtta tatgatactt accataaaaa aatcctccaa ttaccagatg atacagttgt
     5101 tctacctgca cattttaatg gaacatcaat tgctttgaaa catacagtgc caatattaga
     5161 aacacttggt ataataaaga aaagagttaa acttctttct atgaataagg aagaatttat
     5221 tgactatata gcagatacag tacaacctag acctgggaac tataaaacaa taatatcaat
     5281 taacaaaaaa atgcttccat atgatgaaat ggaaatggca gatttagagg ctggtcctaa
     5341 ttcttgtgcg atatcctcat aataacctat catattcgat aaaacatatg aactggaatt
     5401 actaccattt agagaaaaat aatgacctaa atttaaatta ttactaaatc aagtctaggg
     5461 tttgaatcct atcaacaacg caaattctaa ccatctccaa tagcaatcta catctacaaa
     5521 tcctatctcc aatagcaatc tacatctaca aatcctatct ccaatagcaa tctacatcta
     5581 caaatcctat ctccaatagc aatctacatc taca
//
LOCUS       DASVEV010000324         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_768496, whole genome shotgun sequence.
ACCESSION   DASVEV010000324 DASVEV010000000
VERSION     DASVEV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768496"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VF222_14060"
     CDS             <1..448
                     /locus_tag="VF222_14060"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7143598.1"
                     /translation="QYWTLFLSTIFFNLNKKWIILFIFIVILMLIWHIPDIFDYATFN
                     PNIHILQHFSFIIVGILLFVSIRLSYESNSIFYLLSSIGMMVLSGLLLTTTNDIIYTF
                     YSLDSHHTSGNYMFILTILIGLVVFPFYLIQRTLFHIRQSTKNQEK"
     gene            1105..1809
                     /locus_tag="VF222_14065"
     CDS             1105..1809
                     /locus_tag="VF222_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143599.1"
                     /translation="MIQDDNNVYLAATNQTFTESEPRIPSSISDGEETTIMSTNETSQ
                     EAQDLQTQAPFVENVIKSKVGGNKSSSTTTITNSTVSQLYNLRNSYLSEWEKIPFQSI
                     FDTFIDENSAQGYGIFTERNSSIFNPTQRISLYIEPIGFKHEPIIDTKGNKLYLTNIT
                     TIITMEDDKGNEVPSIEDMQSYLFKSYNKITELFLPIDLDLTSSLPTGNYVITYTLTD
                     NISKENFDIQKNITIS"
BASE COUNT          829 a          274 c          273 g          786 t
ORIGIN      
        1 tcaatattgg acactttttc taagtacaat attttttaac ttaaataaaa aatggataat
       61 tttatttatt tttatagtga ttcttatgtt aatatggcat attccagata tctttgatta
      121 tgcaacattt aatccaaata ttcacatatt acaacatttt tcatttatta ttgtaggaat
      181 tttattattt gtctctatca gactctctta tgagtcaaat tctatttttt atttattatc
      241 atcaatagga atgatggttt tatcgggatt attattgact actaccaatg atattattta
      301 tacattctac tctctagata gtcatcatac atcaggaaat tatatgttta ttttaactat
      361 tctaattgga ttagttgtat ttcctttcta tctaatacag agaactcttt ttcacattag
      421 gcaaagcaca aaaaatcagg aaaaatgaaa ctgtaaggat tacttgaact tcataaataa
      481 aattttagtt aatggatttt atattactat acttgagaat atctaaacta gataataatt
      541 agattaataa aaaataccta atggaagcta gaaagaataa tttcaattag atgaatagcc
      601 tatacaaaga ctctagttaa tccgtgattt ttaggtatta aaagaattga tagtatagat
      661 ggtagatgcc taaaactaaa aagaactaca tttgatataa atgaaaaaat aagtaatgta
      721 gaaattttac actaaagaaa aatgtaaatg ataataatac tataaatcaa aatgtcaaat
      781 ttatctttca atcaacaaaa gaaccaataa gggtatttgc tgataaaaag agagagagag
      841 agagagagag aatttaccaa tgattagtaa tttgataaag actttaatga agagaggatt
      901 gacctttaca tatcataata tttctagaat agttagttct ttagtatttt gatattgtgt
      961 aatagttcta tttgtaaatt aacatttata atacaaattc attctttacg tattatgaat
     1021 acattattgt tattgtttat cagtatactc atacttgcac tgtcttcagt gaatttcatt
     1081 tttctaaatg attcgtttca gataatgatt caagatgata ataatgtata tttagctgct
     1141 acaaatcaaa catttacaga atcagaacct agaattccat cttctatttc ggatggagag
     1201 gagactacta taatgtcaac taatgagaca tcacaggaag ctcaagatct tcagactcaa
     1261 gcaccatttg ttgaaaatgt tattaaatca aaagttggag gaaataaaag ttcttctact
     1321 actactatta ctaattctac tgtatctcaa ttatataact tgcgtaatag ttatttatca
     1381 gaatgggaga aaatcccttt tcagtctatt tttgatacat ttattgatga aaattcagct
     1441 caaggatatg gtatatttac cgaaagaaat tctagtattt ttaacccaac tcaaagaata
     1501 tcactatata ttgaaccaat aggctttaaa catgagccca taatagatac aaaaggaaat
     1561 aagttatact tgacaaatat tacaacaata ataacaatgg aagatgataa gggaaatgaa
     1621 gtaccatcta tagaagatat gcaaagttat ctttttaaat cgtacaacaa aataacagaa
     1681 ctatttttgc ctatagattt ggatttgaca tcttctcttc caacaggaaa ttatgttata
     1741 acttatactc tcactgataa catatcaaaa gagaattttg atattcaaaa aaatattact
     1801 atctcataga atataggaaa tttattttat agaaaacttg gttgagcttt aaaactttat
     1861 cctattttta attattatac ctgcagttct tttggtgcat aatcatgtaa acaataataa
     1921 gtattctcat catcatcctc ttaattgtat cttaaaagaa tatactctag gtctaaaatg
     1981 aattgaatac aaatagcttg agagatatct catatataaa tagcatattt gtttaattta
     2041 aatatagaaa aattacaatt tatctacttt tgtaaaataa atttgattgt ttcctgatat
     2101 atcttcttgc catacaacaa ataactcatt agcagtagca gtcaattgtg gattaattga
     2161 at
//
LOCUS       DASVEV010000325         8127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_187212, whole genome shotgun sequence.
ACCESSION   DASVEV010000325 DASVEV010000000
VERSION     DASVEV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187212"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1066..2040
                     /locus_tag="VF222_14070"
     CDS             1066..2040
                     /locus_tag="VF222_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7143600.1"
                     /translation="MVDLIFGRGITDILREMPFYFYFFKNTISIKIFHSQRTIYGSVD
                     VNNVCNLHCSHCYWWLNRKNDEKDLSIADWKKIIQEKFKKQNIFVVTLVGGEPLLRPD
                     IIKLFCEEMPRRVCVVTNGTFPLKRFEGIYFYWISLDGTEEVHDSIRGKGSYSQTRKN
                     ICDYTENQPKRNGKPAWKDIWITMTINTLNYSTAIALAHEWKGKINKIGFQFHTPFTK
                     DDPLWLPFGKIRNNVVNDIISLKKSYPDYVANSEKQISLMKGNWGGIGTTPVQCPSWA
                     ILSLDHMGRVKMPCCIGSANNKGLKPICEQCGLGCYSILVAQGIKGNN"
     gene            complement(2261..2554)
                     /locus_tag="VF222_14075"
     CDS             complement(2261..2554)
                     /locus_tag="VF222_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143601.1"
                     /translation="MAKLKVYKRNSKFIDLVELVGEISSIGLVESLEKYYNKPYEKEA
                     KSIVAGPSFLQFLNKLFQIQISSGDIINLESASHSKYYMLSLSGTWDEIIKLQ"
     gene            complement(3114..3713)
                     /locus_tag="VF222_14080"
     CDS             complement(3114..3713)
                     /locus_tag="VF222_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7143602.1"
                     /translation="MYSNRKFNESPNHFMVLDAVARGMKKIDSISKVTKLSKDEVELI
                     VNDMVAQKILIKNEKKGFFGNKKIELDIADTGMKILNTKKQELSEKSERLQQLYETGD
                     KSQVQGFMDSNRMWIPMMLFSGVMNMVFFASMMSFMGMAMNPAESAATEGQVDQTTTG
                     AEDTGATADNSDSGASEMDTSSGGDAGFEGFDGGGFGDF"
     gene            complement(4011..5078)
                     /locus_tag="VF222_14085"
     CDS             complement(4011..5078)
                     /locus_tag="VF222_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7143603.1"
                     /translation="MDSQYAIDTSSLITEGSLIASENKLKNERLSISKIAFTDKAHPK
                     NIVNEGLIAGQKIAKISIIILLSLGISESLIGYWGGSLVAIADGIDSFSGAMLSFIVL
                     LGLRITNRPPNKKFPYGYQKVESFAALMAAIGMIIIGAIVFYLSYQAFIHPHEIKQPV
                     ITLVILALAGLISFHRSFQMRNLANKYNLLSLKKDATNSIKDGSASIIGFFSVVISTQ
                     FGILQMDAIGGMVIAVYIFSVSYLSLKKSSFILVDSCEDSNLSDRLKKIIMERFSDEI
                     INVKSILVRPTGMAMHAEFHVELDGRRRFSDVDLLLMDIEKVIRSTFPNFASLTIIPH
                     SFKNSNTQFDSITHEIKILSK"
     gene            5701..7086
                     /locus_tag="VF222_14090"
     CDS             5701..7086
                     /locus_tag="VF222_14090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7143604.1"
                     /translation="MENQKSKFQIHSVNNSCQLKFILFTILISSLLYFNVIFATNENN
                     EKLILNDVPLQILDNNNNNVPVSGYTFSNITNITNNKHDSVYAQIAAIDNNVYVVWQE
                     SITTNSKETNYEIYLKKSEDQGNTFSKPINLSNNSGFSEHPQIAVSKNGIFIIWVDNT
                     YSNNTEIMFTKSLDNGTTFSEVKILSNDSKNSNNPEISVSDENVYVVWQDIDQTYSHS
                     NKENLIDKETSSNIMFIGSMDNGNTFNDQIELTNNTYDSYPKVNSYQNYVYVVWNSED
                     KSSSKDSKDSGLFFIRSSDNGESFQNTIRLVYNNFGESQISVLGNKVVIVWGGLHSKN
                     INDIYFVKSNDNGDTFTDPYIIPQKVTKPKDNNYSDKINNPSNVEIANNDHSYTVWQD
                     LISEKNQDIFIANIKNDRESNYMINLSNNTGISECPSIAISKNYMYIVWEDFTPGNHE
                     ILFTKGINNSS"
     gene            complement(7172..7681)
                     /locus_tag="VF222_14095"
     CDS             complement(7172..7681)
                     /locus_tag="VF222_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7143605.1"
                     /translation="MKTLLLLRHAKTSWDDAKVSDDLRPLSETGKRNVYQMGKFLKNT
                     KLIPDLILSSCAKRAKDTSILLAESSGYNKDIDISESLYETATKEYINVISEISNKIN
                     IVLLVGHNPILENLIELITNELIIMETCSLVQIFLPISTWTEIKMNPKCQLIKQIRIK
                     ELASQAVNS"
BASE COUNT         2888 a         1213 c         1106 g         2920 t
ORIGIN      
        1 gtcgatattt atgttatttt ctagtagatg catttgaaag tttcttgcaa gctcttgttt
       61 ttaaagaaat ttttgctcac attttttgcc acaatcaata gttcttgtac tttatttttt
      121 ttacaggttg aatcattatt aatataaaat aaactaaagt gaaaaatatg gttgccatct
      181 cattctaaac tctttacttg aacaatagta aaatttttgt tatccttccc tctttttctc
      241 aaataataat aacaatcttt tttaatgtca tagttaaact cttaagaatt accatgagtg
      301 acataacaca taataataat ccgaatgaca tatgttttta ttgttcacat atatactctg
      361 ttcatttctt acacgacaca cacgtttttg aaagcaatga gcttagtaac actcatgctg
      421 gctgcagtca tgttaattcg gatggaagtt cttgtatttg tcctggcttt aggtctgcaa
      481 atacgaatta taatcaataa tgttaatata gacaaatagt taaaagaaaa ttataaacag
      541 atcaacactt ctatcatttt tttattataa tcaatcatac ttttagatct atataccaaa
      601 tgaactcaat aacggtcttt ttaaaagtac tggaatatta tagctgatta aattaaatga
      661 tcattgttac taatagagga agataacaca attactgtct ataatgagga aaaataattt
      721 agtgtttcag atccaaattg agaataaaat cttttttttg catgacacgt actaaaaatg
      781 gtaaacattt aaaaaccaag aaagaatgga cagataaaat tccttaacag atattttgta
      841 ccttcatatt ctgttaattt ttataatctc tagatctaat tatattagta ctatataatt
      901 taacaacatt tttgtataat ttactaatac tacagaaaaa taggcaatta acattttgag
      961 ataggaaaat aaataaattt taatcgttat taagtttgaa gaattaataa ctgaacaaaa
     1021 ttatcccatc atcatgttta tttaggtggg atttttaaca atggtatggt ggaccttatc
     1081 ttcggacgag gaattacaga tattttacga gagatgccct tttattttta tttcttcaaa
     1141 aatactattt ctatcaagat ttttcattct caacgcacca tctatggttc tgtagacgtc
     1201 aataatgttt gtaatttgca ttgttcccat tgctattggt ggcttaatag aaagaacgat
     1261 gaaaaagatc taagtattgc agattggaaa aaaataatac aggagaaatt caaaaaacaa
     1321 aatatctttg tagtcacact ggtagggggt gaacctttgc tcagaccaga tataataaag
     1381 ttattttgtg aggaaatgcc aagaagagta tgcgtcgtta ctaatggcac ttttccttta
     1441 aaaagatttg agggtatata tttttattgg atatcactag atggaactga agaagtccac
     1501 gacagtatca gaggtaaagg atcttattca cagacaagaa aaaatatatg tgattatact
     1561 gaaaatcaac caaaacgtaa tgggaaacct gcatggaaag atatatggat tactatgact
     1621 attaatactc taaattattc aacagccatt gctcttgcac acgaatggaa aggcaagatc
     1681 aataaaattg gctttcaatt tcatactcct tttaccaagg atgatccatt gtggctcccc
     1741 tttgggaaaa tacgcaataa tgtagtaaat gatataattt cattaaagaa atcatatcca
     1801 gactatgtgg caaattctga aaaacaaata tctttgatga aaggcaactg gggtggtatt
     1861 ggaactactc cagtacaatg tccttcctgg gctatacttt ccttggatca tatgggaaga
     1921 gtcaaaatgc catgttgtat tggaagtgct aataataaag ggttaaaacc tatttgtgag
     1981 caatgcggtc ttggatgcta ttccatactg gttgcacaag gcataaaggg taacaattaa
     2041 accatcgtta gatattaaaa aaaatagaat cacaaataat tataatattg gaatctgtat
     2101 cagatatata acaattttta tttaattata tttttgaagg aataagtagg tcaatactat
     2161 acaattcaag gtcatttata gaatataaca atcatgttcg agttaaagac ataattcttc
     2221 aatttcctac ttctaaatga atagcaaaca aagctctaca ctattgcaat tttataattt
     2281 catcccatgt tccagataga gaaagcatat aatatttgct gtgacttgca ctttctagat
     2341 tgattatatc accagatgat atttgaattt gaaataattt atttaagaac tgtaaaaaac
     2401 taggtcctgc cacaatactt ttagcttcct tttcataagg tttattatag tatttttcca
     2461 agctttctac taaacctata ctactaattt ctcctactaa ttcaactaag tctataaact
     2521 ttgaatttct cttatatact ttgagttttg ccataataaa aatatcctta aaatatttga
     2581 gataatctat aatataatat tatcactcat atctacacaa tactaattta tgctttaatt
     2641 ggaaatagaa aaataagcca actaaaattt aggttttgat agttgtgatt cttactatca
     2701 accgtgattc tcttttttcg tattttgatt catagagctc aatcataata ttactaataa
     2761 gtatcctaac attattataa tatattacat tagagccata aatcaaatat cattgtgtga
     2821 tgcgattcat atctcatatt gagaatattc actgaattaa ataacataaa ttgttttcct
     2881 aaactaactc taatattcta aattgaatac atgtatgatc ctaaatactc attctaattt
     2941 cattcatgta tcctaattat tatttattaa ttaaactaac ataagattaa tataaaaata
     3001 attgtaaata tattgtaaag gttgaaaccc accccccccc ttttttttta tttgaccaat
     3061 tttaactatt atatttaaaa gttatacaat ttgtgtttgc tttactgaca atattaaaaa
     3121 tcaccgaaac ctcctccatc aaatccttca aagcctgcat cacctccact gctggtatcc
     3181 atttcacttg ctccactatc actattatca gctgttgctc ccgtatcttc tgctcctgtt
     3241 gtcgtctgat ctacttgacc ttcagttgct gcactctctg caggattcat tgccattccc
     3301 ataaatgaca tcatagaagc aaaaaacacc atattcataa ctcctgaaaa taacatcatt
     3361 ggtatccaca ttctattaga atccataaaa ccttgcactt gagatttatc tccagtttca
     3421 tagagttgtt ggagacgctc tgatttttca gataattctt gtttctttgt attaaggatt
     3481 ttcattccgg tatcggctat atcaagttct attttcttat taccaaagaa accttttttt
     3541 tcattcttga ttaatatttt ttgggcaacc atatcattta caattagttc aacctcatcc
     3601 tttgatagct ttgtaacttt ggaaatacta tctatttttt tcatacctct agctactgca
     3661 tctaatacca taaagtgatt ggggctttca ttgaactttc gattggaata catatactat
     3721 aattttggta aaagactatt taaattgaat gttgaattta tcattagaca agattataag
     3781 cgtgaattta aacaaagata aaaataattt tataatcttt tttccaataa tctattttgt
     3841 ttatcccaca gtgatcaaga ttctatatct agaattataa ctattttcat tccaatactt
     3901 tggaatacgc tcattatcat ttgtagttaa tatttagata tatttcctta ccacgcattt
     3961 tatagttaaa gaaaaaatcc tatttttttc ttttctttta tcttaacatt ttatttactt
     4021 aggattttaa tttcatgtgt aattgaatcg aattgagtat tagagttttt aaatgaatga
     4081 ggtattattg tcaaactggc aaagttaggg aacgttgaac gtattacctt ttcaatatcc
     4141 attaacaaga gatccacatc actaaatctt cttcttccat ctagttcaac gtgaaattcc
     4201 gcgtgcattg ccatacctgt gggacggacc aaaattgatt ttacatttat tatttcatcg
     4261 ctaaatcttt ccatgattat tttttttagt cgatctgaga ggtttgagtc ttcacatgaa
     4321 tctaccaata taaaagaaga tttttttaat gacaaatatg atacagaaaa tatatacact
     4381 gcaattacca ttcctccaat agcatccatt tgtagaatcc cgaattgtgt agagattact
     4441 acactaaaaa atccgattat agaagcagat ccatccttta tagaattggt tgcatctttc
     4501 ttaagcgaga ggaggttata cttattggct aggtttctca tttgaaaaga tctatggaat
     4561 gatatcaagc ctgccagcgc caaaattact agggttataa ctggttgttt aatttcatgt
     4621 ggatgtataa atgcttgata tgaaagataa aagactatag ctccaattat tatcatacct
     4681 atagcagcca ttaaagcagc aaaactttcc actttctggt atccatatgg aaatttttta
     4741 tttggtggcc tattagtaat tcgtaatcca agtaaaacta taaatgatag catagcgcct
     4801 gagaaagaat ctataccatc tgcaattgcg actagactac caccccaata accaatcagt
     4861 gattctgaaa ttcctaagga tagcaatatg attatagaaa tcttggctat tttttgacct
     4921 gcaattaaac cctcgttaac aatatttttg ggatgggctt tatcagtaaa ggctatcttg
     4981 cttattgata atcgttcatt ttttaatttg ttttctgatg ctattagact accttcagta
     5041 atcaaactgg atgtatctat agcgtattga ctgtccaact attttattaa tatattatga
     5101 atttaataat atttaaagtc ttatgttttt atttgtatct tagtgatatt tatttataat
     5161 ttattagatt atgttaaatc atgagatagt caacaaatta tttattcgat aacagaatgg
     5221 gcttgaatta agaacgtctt gttattttgc tctgtcagta accaatcata atatttttct
     5281 tattgctaag gttatattat atccttaatt gagtttatat atatagataa ttaactacga
     5341 caaagaatag cattacttga tgaggaactc tacacattct aaaatatcgt cgaaccttac
     5401 cgtcaaggat taaaggacat aactgacgct ctttagaaat gctataactg tatttcaata
     5461 gtagatgctc cttctggcaa aaaagtaatt tttactatcg atttaggtag ttcaatatac
     5521 aaattactta tattcaagac ttctgaattt aaagatgcga ttgtaagtaa gaaaaatatt
     5581 tcgaaacatc attaaattca attgtatcta ttttatcttg actttaatgt gtggtgatat
     5641 aataaattaa tccagatatt tatgaatatt gaaatatagt gaacacctat agtttagcat
     5701 atggaaaatc aaaagtctaa attccaaatc cattctgtaa ataatagttg ccaattgaaa
     5761 tttattcttt ttactattct aattagttct ctattatatt ttaacgtgat tttcgcaaca
     5821 aatgagaaca atgaaaagtt aattctaaat gatgttccct tacaaatatt agataataac
     5881 aataataatg ttcctgtctc aggatacacc ttttcaaata taactaacat tacaaacaat
     5941 aagcatgatt ctgtatatgc tcagatagct gcaattgaca ataatgttta tgtggtatgg
     6001 caagaatcta ttactactaa ttcaaaagag acaaattatg agatttatct aaaaaaaagt
     6061 gaagaccaag gaaatacatt tagtaaacca attaacttga gtaataactc tggattttct
     6121 gagcatcctc agattgcagt atccaaaaat ggaattttta ttatttgggt agacaatacc
     6181 tactctaata atacggaaat tatgtttaca aaaagtttgg ataatggtac aacatttagt
     6241 gaagtaaaaa tcctaagcaa tgactctaaa aattcaaata atccagaaat atcagtctct
     6301 gatgaaaatg tatatgttgt atggcaagat attgatcaaa cttattctca cagcaataaa
     6361 gaaaatctaa tagataaaga aacctcatcc aatattatgt ttataggcag catggataat
     6421 ggaaacacct tcaatgatca aattgaatta acaaataata catatgattc ttatcccaag
     6481 gttaattcgt atcagaatta tgtatatgtt gtttggaata gtgaagataa aagtagctct
     6541 aaagactcta aagatagtgg attatttttt ataagaagtt ctgataatgg ggaaagtttt
     6601 caaaatacca ttagacttgt ttataataat tttggagaat cacaaatatc agttctcgga
     6661 aataaagtag ttatagtttg gggagggctt cattcaaaaa acatcaatga tatatatttt
     6721 gtcaaaagta atgacaatgg tgacactttt acggatccat atataattcc acaaaaagtt
     6781 actaaaccaa aagataataa ttattctgat aaaatcaata atccttccaa tgtagagata
     6841 gccaacaatg accattcata tacagtatgg caagatctaa tttcagaaaa aaatcaagac
     6901 atattcatag ctaatataaa aaatgatagg gagtcaaatt acatgataaa tttgagtaat
     6961 aatacaggta tttcagagtg tccctctata gctatttcta aaaactatat gtatatagtg
     7021 tgggaagatt ttacacctgg taaccatgaa atattattta ctaaaggaat aaataattca
     7081 tcataataat taaataggaa aggtatcaat tttatatcgg atttagaatt ttttgtaact
     7141 ttattctttt gataatattt aactaatttc tttaactatt cactgcttgt gatgccaatt
     7201 ctttaattct aatttgttta attaattgac atttgggatt catttttatt tctgtccatg
     7261 tagatatagg taaaaagatt tgaactaacg aacaagtttc cataataatt aactcatttg
     7321 taattagttc gataaggttt tctaggatag gattatgtcc aactaacaaa actatattaa
     7381 ttttattgga tatttcacta attacattta tgtattcttt ggtagcggtt tcatagagcg
     7441 attctgatat atcaatatcc ttattgtacc cgctagattc tgctaataag atagaagtat
     7501 ctttagccct cttagcacaa gaactaagaa tcaaatcagg tattaatttt gtatttttta
     7561 agaatttacc catttgatag acatttcgtt tgccagtttc acttagtggc cgtagatcat
     7621 cagaaacttt agcatcatcc cagcttgttt tagcatgacg aagaagtaat aaagtcttca
     7681 tggatctcct tattcatcat tggataaatt tgtacctcaa attataatct tttgcaatta
     7741 attatataat aacgattagt tctgaatata agaaaaaaaa ttttgatcat aaaaaaaaaa
     7801 tttgtttcta aatgagtaga tactgtttct aacttgaaat aaataaaaaa atagcatctt
     7861 ttactttgtt aaatagtaat ttattttgtc gtcgtcacaa tgtgaaaaga ggaaattggt
     7921 tatataaaaa atataactat tttagatcac tttatctgtt catatataaa taactaacaa
     7981 ttgtgcctac caaagtaaaa gacccaatta atgcagcaat aaatacggta tctgctactg
     8041 atgctagtgg ttcacccatg actgctacga atatcgaata aacaagaata ataaatccaa
     8101 ttaaagcgaa taatcctaac attattg
//
LOCUS       DASVEV010000326         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1547483, whole genome shotgun sequence.
ACCESSION   DASVEV010000326 DASVEV010000000
VERSION     DASVEV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547483"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..951
                     /locus_tag="VF222_14100"
     CDS             592..951
                     /locus_tag="VF222_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143606.1"
                     /translation="MADNIYKNSDDYKREIDKLTSTNNEIGQSNLKKVNPEKDTKESQ
                     TQDKKGSTFENGGSDIKSIVFGLFGLIGFIIIVYSIFVAVIGEPLASVADTVFVAALI
                     GSFTLVSTLVGLIFLKK"
     gene            1560..1880
                     /locus_tag="VF222_14105"
     CDS             1560..1880
                     /locus_tag="VF222_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143607.1"
                     /translation="MEEIVENFAQRLEISPDMARQGISITSRFFLQNSEPVVATGILS
                     MLPSSLTNMFSNDEKQEFKTSQKYIPYDEIIKKISNDCFNGDIQKAKKVYEEAINVIK
                     RQIW"
     gene            complement(2291..>3391)
                     /locus_tag="VF222_14110"
     CDS             complement(2291..>3391)
                     /locus_tag="VF222_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7143608.1"
                     /translation="QNNYYRKVFSGKLLGGSSVYDFGSGERLNTGYPILLQEKPKYFI
                     FVITPTTQIYSHINDILHTERVKGFSLIFGLSFAVLILIIFFIKWNNTLNIEVRKRTK
                     ELRESNRKLSLSNQEIAQANEELKVHDKMQKEFINIASHEIKTPLQAILSASQLLQMY
                     PERQKKFASAIQRNAIRLQRLSNDILDVAKIESRSLKLNKELLDLNQVISNIVEDYKT
                     LIIKDNHSVKLYFKPFHHALFVEADKERIAGVISKLLSNAIKFTKKGEIFVSIEKENN
                     NTNPYALVTVKDTGEGIDPEILPKIFSKFITKSFEGLGLGMYIAKNIVEAHGGKIWTE
                     NNNNKNGNRGAIFYFSLQLSKIVRTSNNNIDK"
BASE COUNT         1230 a          441 c          441 g         1279 t
ORIGIN      
        1 atgcacagac atactaatat tgattggtct aaagtaatag acaataattg atttttaaag
       61 gcgaattact ttaaggatta aagactattt atgctataca tgattttact actaatataa
      121 ttattaaaac tatttcttcc aatggttatt attaagaggc tattatcata gatcattgga
      181 ccaatgattg gtaaagggat ttatccatca taagtcgcta aatcatcata atcattttgt
      241 ctctaattgt catataatat caatctaaag tgagtgaaca aaatcgtcat tcttctgaat
      301 gaacccaatt gtcatatcct ggattgcatc aattgtaata attaagataa atacaattta
      361 tatgaactat gacgataaaa ttatctgaat ttattaaata ttataatttt caataggttg
      421 atacatccag tgtaaagact ctaatctcgc catagacatc tgtgatttgt tcctaaatag
      481 gctatgacat caagtattaa gacggatatt caaaaagtat attgaattta gatgttctca
      541 ctaagattaa ttcttaattg tttttatact attaaatata attatattga aatggctgat
      601 aatatatata aaaattctga cgattataaa agagaaatag ataaactaac ttctactaat
      661 aatgaaatag gtcagtctaa tcttaaaaaa gttaatcctg aaaaagacac taaagaatca
      721 caaacccaag ataaaaaagg atcgacattt gaaaatggtg gtagtgatat taaatcaata
      781 gtttttggcc tatttggctt gattggattc ataattattg tatattcaat atttgtagcc
      841 gttataggtg aaccactagc atcagtagca gatactgtat ttgttgctgc attaattgga
      901 tcctttactt tagtcagtac actagttggt cttatattct taaagaaata aaggatatct
      961 ttatttacca ttttacattt catacaagat tctttcataa taattgactt atttcgtaag
     1021 tttgattttt aacttgttta tcaaattgtt ccttaattat aataaaataa tacattaaag
     1081 ttatttttat tcatttatct attattaatt ttatgctaat tgtaaaatga tttcaaactt
     1141 acctatatta ttatagttat ttctatcaag taatcaagga gaattaatta agagttttag
     1201 taataattta gtaataatta tcaatcaatt actaatttct cttctttaat tagaaaggaa
     1261 ctattttttc cattattatt ttatcgaaat tactcttcct aaaatccaga gttaaaagta
     1321 aatgataatt tttcaatgat ttttataaca ttaaaatctg tttattgtaa aagtggtata
     1381 tctatagtga ttattttttg gtatttaccc cataggatca ttaatatttt gaccattcca
     1441 gtgaattcta tttattattt gaatataaga aaaaatataa tggcagcgaa aaaaattcag
     1501 cattattatg gataaaaata ttaccaattg attaaatatt taattgtcat ttttttgtta
     1561 tggaagagat tgttgaaaat tttgcccaaa gattggaaat ctcaccggat atggcaagac
     1621 aaggaatttc aatcacatca agattttttc ttcaaaattc agagccagta gtagcaacag
     1681 gtattttatc aatgctacca tctagtttaa caaatatgtt ttcaaatgat gaaaaacaag
     1741 aatttaagac tagccaaaaa tatattcctt acgatgaaat aataaaaaaa atatctaatg
     1801 actgttttaa cggcgatata caaaaagcaa aaaaggtata tgaagaagca ataaatgtga
     1861 ttaagagaca aatctggtaa aaataaaata cgtagtaatt aatatggtgt gacaattaaa
     1921 tatatcaaat tattaaaatc tgtaatcagg atgtcatcaa tgtccctaat caaaacacta
     1981 ttaagcaaaa aatctagatc tatatagaat agtcctaaat tacttataaa ctattaattt
     2041 ttatatttct atatgaagaa aacacaaaat aataataata acaataataa tggtaattta
     2101 ttattaattg aaaataaact tagtaaatca atatagtaaa tcaataataa ggataataga
     2161 tcctaatttg ggattacatt tatctaatat agcaaatgaa aaggatgaaa gattaggact
     2221 tgtatagatt cgaaaggatt gatagaaaat tttcgctcat tatataatat aattttaata
     2281 atttcttttt ttatttatct atattgttat ttgatgttct tactatcttg cttagttgta
     2341 aagaaaaata aaatatggct ccacgattac cattcttatt attgttgttt tcagtccata
     2401 tctttccccc atgagcttcc acaatatttt tagcgatata catccctaaa cctaaccctt
     2461 caaaagattt tgtaataaac tttgaaaata ttttcggtaa tatttctgga tctattccct
     2521 ctccagtatc ctttacagtc actaaggcat aaggattagt attattattt tctttctcaa
     2581 tagaaacaaa tatttcgcct tttttggtaa atttaatagc attacttaga agtttggaga
     2641 ttactccagc aattctttct ttatcggctt ctacgaataa agcatggtgg aatggcttaa
     2701 aatataactt gacactatga ttatccttta taataagagt cttataatcc tctacaatat
     2761 ttgatattac ttgatttaga tcgagtaatt ccttgtttaa ttttaatgac cggctttcaa
     2821 ttttagcaac gtctaatatg tcgttggaaa gtctttggag cctaatcgca tttctttgta
     2881 ttgctgacgc gaatttcttt tgtctctcag ggtacatttg taatagttgg gaagctgaaa
     2941 gaatagcttg aagcggtgtc tttatctcat ggcttgctat attaatgaat tccttttgca
     3001 ttttatcatg aacttttaat tcttcattgg cttgtgcaat ttcttgattt gataatgaaa
     3061 gttttctatt tgattctcgt aattcttttg ttctttttct tacttcaata tttaatgtat
     3121 tattccactt tataaaaaat atgattaaaa tcagaactgc aaatgaaagg ccaaaaatca
     3181 acgaaaaacc ttttactctt tccgtatgaa gaatatcatt gatatgtgaa tatatttgtg
     3241 tagtaggtgt tattacgaat ataaaatatt taggtttttc ttgcaacaaa attgggtagc
     3301 ctgtatttag tctctcacca cttccaaaat catatacgga agatcctcct aataatttac
     3361 cggaaaagac tttacgataa taattatttt g
//
LOCUS       DASVEV010000327         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1355537, whole genome shotgun sequence.
ACCESSION   DASVEV010000327 DASVEV010000000
VERSION     DASVEV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355537"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..362)
                     /locus_tag="VF222_14115"
     CDS             complement(84..362)
                     /locus_tag="VF222_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143609.1"
                     /translation="MFIIINPSINDIVITKNPPAKNPYCVFGVCEYPDLYNRKDPNTP
                     TKSISVIIIVSLIVCNDLDGTMVYPLGTFPPSRTAFINPLDNAKKKTR"
     gene            480..1034
                     /locus_tag="VF222_14120"
     CDS             480..1034
                     /locus_tag="VF222_14120"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HEX7143610.1"
                     /translation="MFILFSYMLIGLLIGRFQPLHLGHIEAIKFALRQLSTLVIVVGS
                     AQISYELRNPFTAGERIKMIRNTLIHIDEIDLRNILIIPVPDTNSHALWTHNLDLLVP
                     SYDVIFTNDPFTIALFQERQKKIVQPLLFERQKFSGTRIRENMVKKENWKDLVHFETA
                     KVIEEINGIERLNLLWNKYNEQHQ"
     gene            1101..2489
                     /gene="gatD"
                     /locus_tag="VF222_14125"
     CDS             1101..2489
                     /gene="gatD"
                     /locus_tag="VF222_14125"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HEX7143611.1"
                     /translation="MSTNDDLQPILNNIKVGDIVNVVFNNNSILSGRFMPRYEYSERN
                     HIVIKLPNGYNVGIMLNNIKSITKVSSFSIETEELPNKNVFNSSSLKSQYQNDNNLSQ
                     ISNLPKIALISTGGTIASKIDYRTGGVTSVLSAKDLYTSIPELSLYASIDTEILFNEY
                     SENIGPIHWHLIANKIIEKVNSGNYDGIIISHGTDTMSYTAAALSFALQDLPIPVIIV
                     GAQKSSDRPSSDASSNLIASVIFATQLDYSGVFVAMHGSISDEEIYCHIGTRVRKNHT
                     SKRDAFKSIDISPIAVVQDDRVEIQKIPSNIILHKNKNRNKINKEFNIISKTKFDDRV
                     FLLKYYPGLNPILIENVQKLGYKVIIIEGTGLGHINRNCFSYLKEAIASGILVFMTSQ
                     CIWGRTNLNVYDTGRDLQKIGVISLSNMLSETAMVKAMWLLANYNDLDYIRNKMQENI
                     SNEITYITPIVE"
     gene            2546..4447
                     /gene="gatE"
                     /locus_tag="VF222_14130"
     CDS             2546..4447
                     /gene="gatE"
                     /locus_tag="VF222_14130"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HEX7143612.1"
                     /translation="MNEIILKVGFEIHQQLSTKNKLFCNCKCLEAENYDATFLRKLRP
                     VQSELGSYDLASIFEFTKMLTIKYHSSYTASCLVEADEEPPHEINRDAMDTALIISLA
                     LNSNIINEINVMRKIVIDGSNTSGFQRTMLVSYGGHLQVDGKKIGVQTICLEEDSGKL
                     LSDNRKFREYGLDRLGIPLIEIALEPVTGTPTEIVKIALTLGRLLRASKRVARGLGSI
                     RQDVNISVNNGKGIVEIKGVQRLDQLIKVIEYETIRQHGLILIAEKMNRQRESTGIII
                     GDKIIDITNIFMDSSSKIVQKIISNKGNIFMAIRVKGFKGLFGFEPYPNIRIGKQLGE
                     LVRFYGFGGLFHSDELPNYGITDTEVKKVNEILDIDISNDGFIILGGPKEKMSIAISA
                     ICDRLKNVSSGVPSETRAATIDGKTIYSRPKPGSARMYPETDIAPFKITDDLLVPLKE
                     KVPKPWNETISYLAKKYQLNENLAEKIFDSNYFEIFEILSGLTKVSPSFIASKLTEDI
                     LNLERQGYDSSVLNNNILLDIFKRLDSGLIAKESINLIMEKLMNRTTKSVDESITVLG
                     IKSISDEELEKIIDKIIEDNILIIQEKGMEATSALMGKCMTVLRGKVNGKKINDLINN
                     KLNIILSKP"
     gene            4867..>5168
                     /locus_tag="VF222_14135"
     CDS             4867..>5168
                     /locus_tag="VF222_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143613.1"
                     /translation="MDKKTKSTSKKTNKKSTAAVSRKKTTTPAKKATTKRKTTTTTPP
                     AKKATTKKATTKRKTTTTTPPAKKATTKRKTTTTTPPAKKATTKRKTTTTTPPAKKA"
BASE COUNT         2054 a          683 c          781 g         1650 t
ORIGIN      
        1 aaaaaaggta tttatattta tttttaatgc tatcttttat tgaactatag taaaccaaaa
       61 ctcattattg taatatctgt aatttatctg gttttcttct ttgcattatc aagtgggtta
      121 ataaatgctg ttcttgaagg agggaaagta cctaaaggat ataccattgt accatcaaga
      181 tcattacaaa caataagcga aacaataatt attacactaa tactttttgt aggtgtattt
      241 ggatcttttc tattatatag atcaggatac tcacatactc caaaaacaca atatggattt
      301 ttagctggag ggttttttgt tatcacaatg tcattaatac taggattaat gataataaac
      361 ataaaactta acctctaaaa aaatcatctg aaatgtctaa ttattattcc ttaatatata
      421 ataaataaat cctagattat tattatatta aaaatagact atgtagatcc tttaaattaa
      481 tgtttattct tttttcttac atgcttatag gtttacttat aggtcgattt caaccattac
      541 atctagggca tattgaagcc ataaaatttg cattgcgaca gctctcaaca ttagttatcg
      601 tcgtggggag tgcccagata agttatgaac taagaaaccc ctttacagca ggtgaaagga
      661 taaaaatgat tagaaatacc ttaattcata ttgatgaaat tgatctccgg aatatcctta
      721 ttattccagt acctgataca aatagccatg cattatggac tcataatctt gacttattag
      781 ttccctccta tgatgttatt tttacaaatg atccattcac aatagcttta tttcaggaaa
      841 gacaaaaaaa aattgttcaa cctttattat ttgagagaca aaaattttca ggtacccgaa
      901 tccgagaaaa tatggttaaa aaagaaaatt ggaaagactt ggttcatttt gaaactgcaa
      961 aagtaataga agaaataaat ggtatagaga gattaaatct tttatggaat aaatataatg
     1021 aacagcatca ataatcaata attgtattaa taatgttatt ttgatagaat taaattataa
     1081 ttatatgata agttatagtt atgtcaacca acgatgatct tcaacccatt cttaataata
     1141 ttaaggtagg tgatatagtc aatgtagttt ttaataataa ttctattctc tcagggagat
     1201 ttatgccaag atatgagtat agtgaaagga atcatatagt aattaaactg cctaatggtt
     1261 ataatgtagg cataatgtta aataatataa aatctatcac caaagtttct tcattttcta
     1321 tcgaaacaga agaattacca aataagaatg tattcaattc tagttcttta aaatcgcaat
     1381 accaaaatga taataatctt tcacaaattt ctaatttacc aaaaatagct ctaattagta
     1441 caggtggaac aattgctagt aaaatagatt atcgaacggg aggtgtcacc tctgttttat
     1501 cagctaaaga tctatatact tctattccag aactttctct ttatgcttca attgatactg
     1561 aaattttatt taatgaatat agtgaaaata ttggtccaat acattggcac ttaatagcta
     1621 ataaaataat agagaaggtt aattctggaa actatgatgg tatcataatt tctcatggta
     1681 ctgatacaat gagttatact gcagctgcat taagtttcgc gttacaagat cttcctatcc
     1741 ctgttataat agtcggtgca caaaaatctt ctgatcgacc ttcttctgat gcgtcttcca
     1801 atcttattgc atcagttatt tttgcaaccc agttggatta ttctggggta tttgtggcta
     1861 tgcatgggag tatatctgac gaagaaatat actgtcatat aggtacaaga gtaaggaaaa
     1921 atcatacaag taaacgcgac gcatttaaat caatagatat ttctccaatt gcagttgtac
     1981 aagatgatcg ggtcgaaata caaaaaattc cttccaatat aattttacac aaaaacaaaa
     2041 atagaaacaa aataaacaaa gaattcaata taatatcaaa aactaaattt gatgataggg
     2101 tttttctatt aaaatattac ccaggactta atccaatatt aatagaaaat gtacaaaaat
     2161 taggatacaa agtaataatt atagaaggaa caggcctagg tcatattaat agaaattgtt
     2221 tttcttattt aaaagaagca atagcgtctg gaatattagt ttttatgaca tctcagtgta
     2281 tttggggtag aacaaatcta aatgtctatg atacagggag agatctacaa aagattggtg
     2341 taatatcact atctaatatg ctttctgaaa ctgctatggt aaaagctatg tggcttttag
     2401 caaattataa tgatttagat tatatcagaa ataaaatgca agaaaatatt tcaaatgaga
     2461 ttacttatat aactccaatt gtagagtaat aatttcataa tagttaaaat tatacaattt
     2521 tatcaatgaa tgatgtacta ttgatatgaa tgaaataatt ttaaaagttg gttttgaaat
     2581 tcatcagcaa ttatctacta aaaacaagtt attttgcaac tgtaaatgtt tggaagcgga
     2641 aaattacgat gctacttttt taagaaaatt gcgtcctgtc caaagtgagc taggctcata
     2701 tgacctagca agtatctttg aattcacaaa aatgcttacg atcaaatatc attcttctta
     2761 tactgcaagc tgtttggttg aagcagacga agaaccacca catgaaataa atagagatgc
     2821 tatggacact gcattgataa tctctcttgc tcttaattct aacattatta atgagataaa
     2881 tgttatgaga aaaatagtga ttgacggatc aaatacctct ggatttcaaa gaacaatgtt
     2941 agtttcttat ggtggtcatt tacaggtcga tgggaaaaaa ataggagtac agactatttg
     3001 tttagaagag gattctggga agttattatc agataataga aagtttagag aatatggact
     3061 cgatagactt ggaattccac taattgaaat cgctcttgaa cctgttactg gaacaccaac
     3121 agaaatagta aaaatagctc ttacattagg aaggcttttg agggcaagta aacgtgtagc
     3181 tagaggacta ggtagtatta gacaggacgt aaacatatcc gttaataatg gaaaaggtat
     3241 tgtagaaata aaaggagttc agagactcga tcaactaata aaagtaatag aatatgaaac
     3301 aatcaggcaa catggtttaa ttttaattgc tgaaaaaatg aatagacaaa gagaatcaac
     3361 tggtataata attggagata aaatcataga tattactaac atttttatgg attcatcatc
     3421 taaaatagta caaaaaataa tttctaacaa agggaatatt ttcatggcta tccgtgttaa
     3481 aggttttaaa ggattatttg gatttgagcc atatcccaat ataagaatag gcaaacaatt
     3541 aggagaacta gtaagatttt atggattcgg aggcctattt cattcggatg agttacctaa
     3601 ttacggtata acagatacgg aagttaaaaa ggttaatgaa atattagata ttgatatctc
     3661 caatgatggt tttattattc ttggtggacc taaagaaaaa atgtcaattg cgataagtgc
     3721 aatatgtgat aggctcaaga atgttagctc aggtgttcca tcagaaacta gagcagctac
     3781 gattgatgga aaaacaatat acagtagacc aaaaccagga tcagcacgaa tgtatccaga
     3841 aacagacata gctccattta aaataacaga tgatcttctt gttcctctga aagaaaaagt
     3901 tccaaaacct tggaatgaaa ctatttcata cttagcaaag aaatatcaat taaatgaaaa
     3961 tttagctgaa aaaatttttg attctaacta ttttgaaata tttgaaatac ttagtggttt
     4021 aactaaagtc tctccatcat ttatagcatc aaaactcact gaagatattt taaacttaga
     4081 aagacaaggt tatgattcta gtgtattaaa taataatatt cttttagata tttttaaaag
     4141 actagattct ggtcttattg ctaaagaatc aattaatttg attatggaaa agttgatgaa
     4201 taggacaaca aaaagtgttg acgaatcaat cactgttctt ggaatcaaat caattagtga
     4261 tgaagaactt gaaaaaataa ttgataaaat tatagaggat aatatattga ttatacaaga
     4321 aaaaggaatg gaggcaacga gtgctttgat gggaaaatgt atgactgttc ttcgtggcaa
     4381 agttaatgga aaaaaaataa atgatctgat aaataacaaa ttaaatatta tattatcaaa
     4441 gccgtaaaaa atgaattatt tttataaagt ttattttaat ttttgaattt gatcatattt
     4501 caaattaaac agaatttttg tatttgaatt aaaaaggaat gaaatttaat ttattatttt
     4561 acaaaataaa tatactatta tatttggcta cttcaatcaa atgagagaac atattttgat
     4621 attctaaata catgtttttc tattgaaatt taaagattga atgtttagta tgataaatgc
     4681 ttaaacagaa aataaaatta aaaataatct cttataaata aattatctta ttaattttag
     4741 tgatactttc tttaattagt tttttttatg tttataataa ttggagacta tattcaatag
     4801 aaaaataatt atcttattct gtttccgatt aatataagaa taagtagttt aattatatac
     4861 ttgtctatgg ataaaaaaac aaaatccact agtaaaaaaa caaataaaaa atctacagct
     4921 gcagttagta gaaagaaaac aacaacacca gcaaagaaag caactacaaa aagaaaaaca
     4981 acaacaacaa caccaccagc aaagaaagca actacaaaga aagcaactac aaaaagaaaa
     5041 acaacaacaa caacaccacc agcaaagaaa gcaactacaa aaagaaaaac aacaacaaca
     5101 acaccaccag caaagaaagc aactacaaaa agaaaaacaa caacaacaac accaccagca
     5161 aagaaagc
//
LOCUS       DASVEV010000328        18034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_386781, whole genome shotgun sequence.
ACCESSION   DASVEV010000328 DASVEV010000000
VERSION     DASVEV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386781"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF222_14140"
     CDS             <1..246
                     /locus_tag="VF222_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143614.1"
                     /translation="KVYQNMTGLADTRVYTNLSSLGDKVFPNMSSLESSKVYQNMTGL
                     ADTRVYTNLSSLGDKVFPNMSSLESSKVFPNMSSLSQ"
     gene            complement(758..1177)
                     /locus_tag="VF222_14145"
     CDS             complement(758..1177)
                     /locus_tag="VF222_14145"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX7143615.1"
                     /translation="MTQRPTGITIISVLMIIGGIILLFTGITPLFLGPLISIESSSDY
                     AISELGMLITIGGLVLVGLGIASFIVSWGLLKGKRWARTITLIISIITIIFAIISLAS
                     SGDLIHIISIIIYGIIIYYMFTDKVKLFFGKVKEPTT"
     gene            1497..3089
                     /locus_tag="VF222_14150"
     CDS             1497..3089
                     /locus_tag="VF222_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7143616.1"
                     /translation="MLKPYDDNFKFIRSKIGFKIGILVFIQIIFIITSFIILTYYQSQ
                     STYLGNSINIAGKNRFLTSNLMFSITEYFMENNSDSSRINSAIDQLESNILVLKQGGE
                     ISDIDLKPLPSEFLNDWDNIYQQWILLKTILTNTIIKENQIIKPAVLVTTTTSSTEEK
                     GIDKFINTIQTGALPLVDSSNVLVTKLGIYSKNSSQHSIFLHELFALLNIAVISAFVI
                     YLSRKILKPIFALTTATSAVTKGNLDVSVKSKGNDELSRLSESFNLMVNSIKNFITKQ
                     NELTKKLEKANEELHHKYLIKEEFINVASHEIRSPIQTILGLSELLHFRRIKNSNNIQ
                     DINIEKHEEIVEVIIRNCRRLMQLTSDILDVAKIERGSLILKKEKFNLKELILEILTE
                     YEHTIKNRNNVKFFFESPDDNHNMLEIKADKGRVSQVLHNLLSNAIKFTKEGKITVIV
                     ERKENKIIVSIKDTGTGIDQEILPKLFTKFATYSLGGSGTGLGLFISKSIIEKHGGKI
                     WATSDANNGKVRGSTFSFSLPI"
     gene            3229..3612
                     /locus_tag="VF222_14155"
     CDS             3229..3612
                     /locus_tag="VF222_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7143617.1"
                     /translation="MQKGRVLIIDDNEDVNMLFKIFLEYEGYKVDVYTDPIEALYYFK
                     KGLYDLILLDLKMPKMDGILMFRGLKKIDEQVIICLTTANAQYIQELKKEIPNIDDTV
                     ITKPIILKDLKNKIDSLLENKNNNK"
     gene            3965..4426
                     /locus_tag="VF222_14160"
     CDS             3965..4426
                     /locus_tag="VF222_14160"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7143618.1"
                     /translation="MTTVKYEIDIQAPVEQVYEYYTNPSNIKETWPQDIVKESQSLSG
                     PKNEEGSEMKVKGEYMGREDEMILRVTDKEQNKRLVTEQKDGPFKYWKSVQEFNGSYN
                     TTHIRHSIDYELPSSGKIANFLSGDGANNKIEKGLQQAAQTVKQKLESKSV"
     gene            complement(4711..6387)
                     /locus_tag="VF222_14165"
     CDS             complement(4711..6387)
                     /locus_tag="VF222_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7143619.1"
                     /translation="MNSVIERPPTSILFSFAIGIAAGLVSIAVSVMIKVFVGGLFLPE
                     LASQTLFSLTPGEFESQAIDTLGPLAKYSAFIGSIIVTIIIFGLIGIFLIKLYNKLRW
                     NSYILKFISSFAFTYSILVIISIVMVTIIQVRSGSQTISIQHIILSFIPTQIAFGLIF
                     SLFSRKTKMKDIVQDPYQPAGRENTTVVNYNRREFIRLIIASVVSIPVIYFGLNRLFS
                     PQEVAQQQIQSDVSNLLPPQSGSIPTEFKDPTLAPLLASEITPTYLFYRIDINPIVPE
                     VDVNSWSLLIKGMVGNPLKLSYEEIKSMSAIEEFVTLECISNKIGGDLTGTALWKGVR
                     LKDILEKSKILPDVKYIVFRCSDGYDVGIPLDKGLMNETILAYEMNLAPLTSKHGFPI
                     RAIVPGLYGMMNPKWITEIELVDKVYEGYWQRNGWSNIAEYNTGSSIVIPGQAPIRNR
                     FRDLDENSNTNSRSSGKRVPIAGIAFGGDRGISKVEVSTDGGTTWKTATIKEPLSRYT
                     WVLWTAGFIPEGVENYKIVVRSTDKNGKVQTSELNQPFPDGATGYHTISV"
     gene            complement(6531..6911)
                     /locus_tag="VF222_14170"
     CDS             complement(6531..6911)
                     /locus_tag="VF222_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143620.1"
                     /translation="MMTTTKISYIKIRYIMIFVVLVFVSIDSLSIIKVFSSTNDLAAI
                     HQNNSGSINKSDSMMLPGKNMTFGVSLDNAKMHLMEARMDLKENDIDGALMQLNMTTN
                     NINMHEKEMADMMKMMDKMEEVYK"
     gene            7212..8507
                     /locus_tag="VF222_14175"
     CDS             7212..8507
                     /locus_tag="VF222_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX7143621.1"
                     /translation="MHKEANSYSSIRSAFFELRKNITVQPMYETVNTNNELLGRILYN
                     AINSTVTIPLYDSSHMDGFAVFYNDIRGASNLKPVTLKVMNEVKLGNTKSESLQPGQA
                     ARIPTGGYLPTKSDTVVPIEYTHFNVTDRSVKIFSEFTKGSFISPAGKDITKNKLVFK
                     KGHLLRIQDIALLNIMRIKKLNIYKKPKVAIIPTGSELTNDIDEFAYGKVLNTNGQII
                     FTLIEASGGIPVDLGITSDNIKMIKSKINNALSQYDIVLTIGGSSVGHKDLIVETIHS
                     MGKPGILAQGIRLDRGRVTKVAALKSKPIIILPGPIQGAVNAFIVFALPLIRTLIGLS
                     HNNNAFINAQITDNWIARKKFQNFIKILYVRLSSSKTNNSIRAEPITGETSNMTVLTR
                     ANGYVLVSEKITNIEKGNNVQVNLLPGFSFASSNPMDFL"
     gene            complement(8512..9288)
                     /locus_tag="VF222_14180"
     CDS             complement(8512..9288)
                     /locus_tag="VF222_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7143622.1"
                     /translation="MNDILLIILAPIFFIISFFYSSVGFAGGSSYIAILVLVGVNLYT
                     VPPISLALNIIVSSAAFFNYLKAGHLSLRLSFPLLISVPFAFIGGSIVLPEKMLTLIF
                     VISLFSASAALLLSRVTRRITFQHEKIRKMNLNFFKTALITSPLGALLGSISGLVGIG
                     GGIWLSPILIISGLANPKQAAASASLFILTNSISGFIAHTITKDIDFDLIIPLSIVVL
                     IGGLMGSRLGAFKFDHHKIIIIIGILVALAALNLATRLFK"
     gene            complement(9284..10231)
                     /gene="moaCB"
                     /locus_tag="VF222_14185"
     CDS             complement(9284..10231)
                     /gene="moaCB"
                     /locus_tag="VF222_14185"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEX7143623.1"
                     /translation="MNEINPYNGMFDVSRKRDTLRIATAQAIIKVSPASIDLIKNKRT
                     PKGDIIEAAKISAALGAKKTWELIPYCHSIPIDHISTQILLYESSIEIKVGVKSVWKT
                     GVEMEALTGCCTAALTIYDMLKPIDDHLIIESIKLLDKTGGKRDFIESPDKKITIAIF
                     VISDSTFNGTRVDKSGKMIVNRLENVDITKNIEIVGYEILPDSVDKIKAYLIKYSDEK
                     KVDLIITSGGTGFSKRDVTPEATKMVIDKEANGISEAIRSYGQRRTPFSMLSRGMSGT
                     RGETLIVNLPGSTKAVSESLDLLLPSIFHVFNMLGGKGH"
     gene            complement(10224..10658)
                     /locus_tag="VF222_14190"
     CDS             complement(10224..10658)
                     /locus_tag="VF222_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX7143624.1"
                     /translation="MVGNYMISITKDTIDQIKILDQIKDDSCGAIVSFIGFVRDHNSK
                     GKVEGIFYEAYDEMLEEILKKIEREVFKNWNIKKFIAVHRTGYLQVGEISVVVGVSSE
                     HRKEAFEACSYGIENIKKRCPIWKKEKTQFGNEWIKGVKISE"
     gene            complement(10658..10933)
                     /locus_tag="VF222_14195"
     CDS             complement(10658..10933)
                     /locus_tag="VF222_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7143625.1"
                     /translation="MNISEYTTLKIRLFGILREKMDSDNLIISIHDTSISLKELRKYL
                     EELYPSLGLNEINSVFAVNKVIRNEDNQDINVKPSDEIAIIPPISGG"
     gene            complement(10939..11964)
                     /gene="moaA"
                     /locus_tag="VF222_14200"
     CDS             complement(10939..11964)
                     /gene="moaA"
                     /locus_tag="VF222_14200"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7143626.1"
                     /translation="MIQNNSPQILKDNFNRVARKLRISVTDKCNMKCIYCMPNGNIKW
                     FDNKEILNFQEIVRIAAIFSQLGITKIRLTGGEPLLRPSLENLIRDLTKIENIKTISM
                     TTNGILLEDKISQLKSAGLDTLNISLDTFREERFRTLNGISNLNKVLRGIQKASELGL
                     KIKINTVIIRNWNDDEVINFVKFAQDTRLSVRFIEFMPLDGSGIWNHNLVVTKQEIIK
                     KIKSNFHNVISFDENNNSSFQSDPATIYNFNDGKGKIGFISSITEPFCSNCDRVRLTS
                     DGRFLTCLFEKEGYDLKKMIRNAKTDQEIMDYIVSCHKKKPEGIVQLIRINGLRPSLN
                     LMHTIGG"
     gene            complement(12012..12428)
                     /locus_tag="VF222_14205"
     CDS             complement(12012..12428)
                     /locus_tag="VF222_14205"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7143627.1"
                     /translation="MKKKFPDILFNWGMFLKKLTIVGLFKSQVNIEDMLQIESRQEVA
                     TQPRTPCYKLGVKFGRINILQKILEGDKSGIYFKVVKEGEIGTDDSIKLIKKDNNNND
                     VTIENIVELITEYKDNKFTYGKSSLRAPKMLETIFS"
     gene            12658..13068
                     /locus_tag="VF222_14210"
     CDS             12658..13068
                     /locus_tag="VF222_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143628.1"
                     /translation="MNTIAIVKEDFEMFDKDKNKNSKKAVASLKEKNHIHILDLDDGE
                     FAFMEWLNSENKNSAANHIILKRYEKGKECILNDNTLHSYIKVKMNDNAPFCTYCQDD
                     SCAHVGFTICVMQLFERKEILLGEDENMLDLLKD"
     gene            13412..13948
                     /locus_tag="VF222_14215"
     CDS             13412..13948
                     /locus_tag="VF222_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143629.1"
                     /translation="MSSNQILNNKKSRKPKKTEKEFPKKGDNKTREEQKSHRKLPEPI
                     IYQGENRRNILLAEDDDEFFKQHREQTMREARAITNYFLELHKDMINSYNSEYSNLLQ
                     NIFNSSWDDFTIPGRFTEYPSDIKNTYSILSSNRDNSLKLIDNIMTQNLDTFIQSIEL
                     TQRFYKDVIESYLNCIKK"
     gene            14608..14910
                     /locus_tag="VF222_14220"
     CDS             14608..14910
                     /locus_tag="VF222_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143630.1"
                     /translation="MKSTINLIIVAMLFVGPIVGSFSIYGQETTTNNVKDQIQTTAEQ
                     IKNMADEVANNSTGTINQTEAKSILMQLGEAARKTALAGADILSNLSGEIKEGLRN"
     gene            15387..15638
                     /locus_tag="VF222_14225"
     CDS             15387..15638
                     /locus_tag="VF222_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143631.1"
                     /translation="MKSKICSIVISSILAFALIAGILSTFVNTVAQEDGRDTELGGDP
                     ELGGDTGMTESDLGGNDTGMSISDLENDTEITNGQMDNE"
     gene            complement(16273..16569)
                     /locus_tag="VF222_14230"
     CDS             complement(16273..16569)
                     /locus_tag="VF222_14230"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX7143632.1"
                     /translation="MSFYIILWNYTDQGIKNIKESPKRVNTFKSKLENTGGRLIDTYY
                     TFGKYDGVSVVEAPNDETLMSCLLSIGSQGNARTVTLKAFSYTEATKIMENIRQ"
     gene            17403..>18034
                     /locus_tag="VF222_14235"
     CDS             17403..>18034
                     /locus_tag="VF222_14235"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX7143633.1"
                     /translation="MNEHKSSLLGSKISRTGDILRKTALDQIDEYQLKFSKVTDFYNY
                     LFKDLIVDKYDFTKASYTAKNFFDKDTLHFVAIDGTEYSKPLFDMVIFFAGAYSCNGS
                     IEFSEQEKDKVKIKYQDRFIDQSLDISSCIPVYIDKIPEIDHTFHDKDGKVNLMKPMT
                     EETIMNNTNIANFLMTFSEFYLSYKYASTKKYDIIFMDRSLSNMYSSLLY"
BASE COUNT         6580 a         2646 c         2512 g         6296 t
ORIGIN      
        1 aaagtatatc agaatatgac tggtcttgca gatactagag tatatactaa tctgtccagt
       61 ttaggtgaca aagtatttcc taatatgtct agtcttgaat cctccaaagt atatcagaat
      121 atgactggtc ttgcagatac tagagtatat actaatctgt ccagtttagg tgacaaagta
      181 tttcctaata tgtctagtct tgaatcctcc aaagtatttc ctaatatgtc tagtcttagt
      241 caataaataa tatagttctt caatctaaac tatcagaaaa taactcaatc taaagatata
      301 tttcagatat taataacaaa gttgttattt tgtcttaatt tatattcaat ggtttattta
      361 ttaatatcat tgcattttgt caatccttga ggactccttt attccttaac taataaggat
      421 taatccataa ctatttagta ttgactatat tagtgggatg taaaccaaat ttgacctaaa
      481 tatggatacc tctaaaataa tatttttaaa ttgtatatgc taaagaatga atatgattgt
      541 tactctagca gtcattcatt cattcatagt attagctaca tctaatgacc tagggctgta
      601 aaatttcgag aacatatgaa gaacgcttat ggattggata tgcgcaatca ttataaagca
      661 ttacgaatta atagtcaatt ggaagaaata atctcctata tctttttctt tttattttct
      721 ttaattatat gatttaacaa aaattttaga aaataaatta ggtagttggc tctttaacct
      781 taccaaaaaa taattttact ttgtctgtaa acatatagta aattattata ccatatatta
      841 ttattgaaat aatatgtatt aaatctccac tacttgcaag agaaataatt gcaaatatta
      901 ttgtaattat gctgattatg agtgtaattg ttcttgccca tctttttcct tttaaaagac
      961 cccatgaaac aatgaaactt gctattccta atcccactaa tactaagcca cctattgtaa
     1021 taagcattcc taattcacta attgcataat cagaagaaga ctctatgcta attagaggac
     1081 ctaaaaataa aggagtaatt ccagtaaata aaagtattat tcctcctatt atcataagaa
     1141 ctgatattat agtaattcct gttggacgtt gagtcacaag acaactaaat tccggttgaa
     1201 tttaaatatt acttgatttt acaaagtaga ggttcattag gtgcttatgg gtgtggagtt
     1261 tgtaaaacat ttgcgaagaa aaacatagca tttgatataa tagctggaac atcaaggtag
     1321 caattaacag agttaactaa tagagtaaca aattgttgct ctaacttagg aaataccaat
     1381 aacaaagaac taattgataa tacaagtaca caaattatcg gatatattta aaagagttaa
     1441 aaataactat agatcagaag gaataagccc aaatatatcc atcatgttat ttagtcatgc
     1501 taaagcctta tgatgacaat tttaaattta tacgttccaa aataggtttt aaaataggaa
     1561 tattagtttt tattcagatt atctttatta ttactagttt tattattctc acttattacc
     1621 aatcacaatc aacctatttg ggaaattcaa taaatattgc aggaaagaat aggtttctca
     1681 catctaacct tatgtttagt ataacagaat attttatgga aaataatagc gattcttcta
     1741 gaattaattc tgcaatagac cagttggaat caaacatctt ggtattaaaa caaggtggag
     1801 agatatcgga catagatctt aaaccattgc cgtcagaatt tttgaatgat tgggacaata
     1861 tatatcaaca atggatatta ctaaaaacaa tcctaacaaa cactataatc aaagaaaatc
     1921 aaataatcaa gccagcagta ttagttacaa caacaacttc ctctacagaa gagaaaggaa
     1981 tagacaaatt tattaacaca atacaaacag gtgccttacc tttagtcgat tcatcaaacg
     2041 tattggttac aaaattaggg atctattcaa aaaatagttc tcaacattcg atatttttgc
     2101 atgaactctt tgctctatta aatattgcag ttattagtgc tttcgttatt tacttgagta
     2161 gaaaaatatt aaaaccaata tttgctctca ctactgcaac ttctgcggta actaaaggaa
     2221 atcttgatgt atctgttaaa agcaaaggca atgatgaact ttcacgtcta agtgagtcat
     2281 tcaatttaat ggtgaactcg ataaaaaact ttatcacaaa acaaaatgaa ttaacaaaga
     2341 aacttgaaaa agcaaacgaa gaactacacc ataaatatct aataaaggaa gaattcataa
     2401 atgtagcctc acatgaaatc cgaagtccta tacaaacaat actaggtcta tctgaattat
     2461 tgcatttcag gaggataaaa aatagtaata acattcaaga cataaatatt gaaaaacatg
     2521 aagaaattgt agaagttatt attagaaatt gtagaagact tatgcaacta actagtgata
     2581 tattggatgt tgcaaagatt gaaaggggat ctctaatttt aaagaaagaa aaattcaatc
     2641 taaaagaatt gatactagag atcttgacag agtatgaaca tacaataaaa aatagaaata
     2701 atgtaaaatt tttctttgaa tctcctgatg acaaccacaa tatgctagag ataaaggcgg
     2761 ataaagggag agtatctcaa gttcttcata acttgttaag taatgccatc aagtttacga
     2821 aagaggggaa gattactgtt attgttgaaa gaaaggaaaa taagattatt gttagtataa
     2881 aggacacggg aacaggtata gatcaagaga tacttccaaa actttttaca aaattcgcta
     2941 cgtattccct gggagggagt ggtactgggt tggggttatt tatttctaag agtataatcg
     3001 aaaaacatgg tggaaaaata tgggctacta gtgatgcaaa taatggcaaa gttagagggt
     3061 caacgttctc attcagtctc cctatttagt cattatttat ttaaaaataa tatctttttc
     3121 ggcgccgaaa aaattggtta agtaaaaatt ttttatatag ctgtaacaac tttctagatg
     3181 aatattttga tatagaataa ttgttcatgg gtttgccagg caaggtaaat gcagaaggga
     3241 agagtattga taatagatga taatgaagac gttaacatgc tatttaaaat atttttagaa
     3301 tatgagggat acaaagttga tgtttatacg gatccaatag aagcattata ctattttaaa
     3361 aaaggtctgt atgatttgat tctcctagat ttgaaaatgc caaagatgga tggaatcttg
     3421 atgtttagag gattaaagaa aatagatgag caagtaataa tttgtttaac tactgctaat
     3481 gcacaatata ttcaagaatt aaaaaaggag attcctaata ttgatgatac agtaattaca
     3541 aaaccaatca tattgaaaga tctaaaaaat aaaatcgatt cacttttaga gaataaaaat
     3601 aacaataaat agattacttt tttgtagata tgtttttctc tctctaagag tttagttaaa
     3661 agagtcacat ttttcaaaca aaaaagttaa agttaaattt gtttgaacag atcctaacta
     3721 gatgtatact ctaagtgtta agcttaaatt tttagttata gaaagaagtc atattggtta
     3781 actgacaaag caacttttac aactaaatat aatttgagct tgatatatgt tataaacaag
     3841 cagaattgat atttttaatt gtcaaaatac ccacatcaga tttgaaacat gacgagagaa
     3901 caatagtgcc caactggtat atcatttcac ccacctttat aagttatatt tcacagatat
     3961 caacatgaca acagtcaaat atgagataga tatacaggct ccagtagaac aagtatatga
     4021 atattatacc aatccaagta acataaagga aacatggcca caggatattg ttaaagaatc
     4081 acaatcacta tcaggtccta aaaatgaaga aggttctgaa atgaaagtga aaggtgaata
     4141 tatgggaaga gaagatgaga tgattttaag agtaactgat aaagaacaaa ataaaagact
     4201 agtaacagaa caaaaggatg gcccatttaa gtactggaaa agcgtacaag agtttaacgg
     4261 aagttacaat actacacaca taagacattc tatcgattac gaattaccaa gttcaggaaa
     4321 gattgcgaac tttctttcag gagatggagc taacaataag atagaaaagg gattacaaca
     4381 agcagctcaa acagtaaagc aaaaactaga atcaaaatct gtctagtcgt agcacaaata
     4441 cgaatctaag ctgataacct cctatatttt taccattaaa tatttccaaa ccattactta
     4501 taatagaaac gttagttatc gatgcttatt attctgaaaa gaattaatat tattttctat
     4561 gtaataacaa aatatctact tgaaaagttt aacaattatt ctactatgag ttattttaca
     4621 aattaagtct gaatacgatg tgatttttta tgtttatgta ttaagttctc caacttattc
     4681 cactagttta aatttttagt aaattgatga ttatacactt attgtatgat accctgttgc
     4741 tccatctgga aatggctgat ttaattcgga agtttggact tttccatttt tgtctgtaga
     4801 tctaaccacg attttataat tttctactcc ttctggaatg aatcctgctg tccataatac
     4861 ccatgtatac ctagaaagag gttccttgat tgtagcagtt ttccaagttg taccaccgtc
     4921 tgtactaact tctacttttg aaattcctct atcccctcca aatgctattc ctgctattgg
     4981 cactctctta ccagatgaac gagaattagt attagaattt tcatctaaat ctctgaacct
     5041 attccttatt ggtgcttgtc cgggaataac tatggaggat ccagtattat attcagcaat
     5101 atttgaccaa ccatttctct gccaatagcc ctcgtatact ttatcaacta attctatttc
     5161 tgtgatccat tttggattca tcattccata taagcccggt actatagctc taataggaaa
     5221 tccgtgctta gaggttaatg gagctaaatt catttcatat gctaatattg tttcattcat
     5281 aagcccttta tccaatggga tacctacatc ataaccatca gaacatctaa atactatgta
     5341 tttgacatca ggaagaatct ttgatttttc aagaatatct ttgagccgta ctcctttcca
     5401 taatgcagta ccagtgagat ctccacctat cttattactt atacattcta gggtaacaaa
     5461 ttcttcaatt gcagacatag attttatttc ttcataactc aattttaatg gattaccaac
     5521 cattccttta atcagcagac tccaagaatt tacatctacc tcaggaacaa taggattaat
     5581 atctattcta taaaatagat aggtaggtgt tatttctgaa gcaagtaatg gagcaagtgt
     5641 tggatcttta aattcagtag gtattgatcc tgattgtggc ggcaataaat ttgagacatc
     5701 agattgaatt tgttgttgtg ctacttcttg tggtgaaaat aatcgattta atccaaaata
     5761 tataacagga atagaaacca cagatgcaat aattaatcga ataaattctc ttctattgta
     5821 atttactact gtagtattct ccctccctgc tggttggtaa gggtcttgta ctatatcttt
     5881 catttttgtt tttctcgaaa ataatgaaaa aattaaccca aaagctattt gtgtaggaat
     5941 aaaagaaaga ataatatgtt ggattgaaat tgtttgagat ccacttctaa cttgtattat
     6001 tgttaccatt acaatagaga taataaccaa aatactgtaa gtgaatgcga aagaagatat
     6061 aaattttaat atatagctgt tccatctgag cttgttatac aatttaatta gaaaaattcc
     6121 aattagacca aagattatta ttgtaactat tatagaccct atgaaagcgg aatattttgc
     6181 gagaggaccc aaagtatcta ttgcttgaga ttcaaattct cccggtgtaa gtgaaaataa
     6241 tgtttgtgat gcaagttctg gcagaaataa tcctccaaca aatactttaa tcatgactga
     6301 tactgcaata gatacaagtc ccgcagcaat tcctatcgcg aatgaaaata agatagaagt
     6361 tggtggtctc tctatcacag aattcattag ataataactg atttaaggtt ttttagttca
     6421 aataaataat tatataaaaa ctagtattat tattatagaa tctgataaac gtctgtttaa
     6481 aagttaatat taattaattg ttagtttaat gaaatattat atttaatttt ttatttatat
     6541 acttcttcca tcttatccat catcttcatc atatctgcca tctctttttc gtgcatattt
     6601 atattattag tggtcatatt caattgcatc aatgctccat caatatcatt ctctttgaga
     6661 tccattcttg cttccattaa atgcattttt gcattatcaa gagatacacc gaaggtcata
     6721 ttttttccgg gcaacatcat actatcactt ttatttattg atccactatt attttgatga
     6781 atagctgcaa gatcattagt tgaagaaaat accttaataa tagataaaga atctattgaa
     6841 acaaatacta aaacaacaaa gatcattata tacctaattt ttatgtatga aatctttgtt
     6901 gttgtcatca ttaaatactt tcacaaatat tatttaaatg tactatggtt attattcttt
     6961 aatccattta taaatattaa atcatcgtct tttctatctt tatcaaattg gtgaatcata
     7021 gaaaaagata ttaatatatc aagtcagaaa ttatcattct tatttattaa ataactattt
     7081 tatagtatca gtgtatttga ttataattat aattttgagt tggataaata acattttata
     7141 aaaaataata ataataatga tttttataaa ttaaagaatc atattatgag tttttatttt
     7201 aagaagtact catgcataaa gaagcaaatt catattcatc catccgaagt gcctttttcg
     7261 aattaaggaa aaatattact gtacaaccaa tgtatgaaac agtcaataca aataacgagt
     7321 tattgggtcg gattttatat aatgctatca attcaactgt cactattcca ttgtatgatt
     7381 cgtctcatat ggatggtttt gcagtttttt ataatgacat tcgtggtgct tccaatttga
     7441 aacctgttac cctcaaagta atgaacgagg ttaaacttgg aaatacaaaa agtgaatctt
     7501 tacaacccgg acaagctgcc agaattccaa caggtggata tttacctaca aagtcggaca
     7561 cagttgttcc tatcgaatat acacacttta atgtaacgga tagatcagtt aagatttttt
     7621 ctgaatttac caaaggatct tttatttcac ctgcaggaaa ggatattact aaaaataaat
     7681 tagtttttaa aaaaggacac ttattacgaa ttcaagatat agctttgtta aatattatga
     7741 gaattaaaaa actaaatata tataaaaaac caaaagttgc aataattcca actggaagtg
     7801 agttaaccaa tgatattgat gagtttgctt atggaaaggt tctcaatact aatggtcaaa
     7861 taattttcac cttaatagag gcttctggtg gaatacctgt ggatctagga ataacatctg
     7921 acaatattaa aatgattaag agtaagataa acaatgccct atctcaatat gatattgttc
     7981 ttacaatagg aggctcgtca gttgggcata aggatctcat tgtagaaact atccattcta
     8041 tgggaaagcc aggaattcta gctcagggga ttagacttga tcgaggaaga gtaactaaag
     8101 ttgcagcact gaaatctaaa cctattatta tattacctgg tcctattcaa ggagctgtta
     8161 atgctttcat agtatttgct ttaccactaa ttagaacttt gattgggtta tcacataata
     8221 ataatgcgtt tataaatgct cagattaccg ataactggat agctagaaag aaattccaaa
     8281 actttataaa aatattatat gtccgtttat caagttcaaa gactaacaat agcataaggg
     8341 cggaaccaat aactggagaa acttcaaata tgacagttct gaccagagca aacggttatg
     8401 tactagtgtc tgaaaaaata acaaatattg aaaagggaaa taatgtacaa gtaaatttac
     8461 ttcctggatt ttcttttgca tctagcaatc ccatggactt tttgtaattt attacttgaa
     8521 taatcttgta gcaagattta aggctgctaa tgcaactaaa attcctatta tgattataat
     8581 tttatgatga tcaaacttga atgcgccaag tctagatccc attaaacctc ctatcagaac
     8641 aacaatggac aatggtatga tcaaatcaaa atctatatcc ttggttatag tatgagcaat
     8701 aaatccactt atagaatttg ttaatataaa taaactggca gaagctgcag cctgtttggg
     8761 attagctaga cctgagatta tcaagattgg tgaaagccat atccctccac ctattccaac
     8821 aagaccggaa atgctaccaa gaagagcacc taatggagag gttattaatg cagttttaaa
     8881 aaaattcaaa ttcatttttc ttatcttttc gtgttgaaat gttattctcc ttgttactct
     8941 agacaatagc aatgcggctg aagcagaaaa aagagatatg acaaaaatta atgttagcat
     9001 tttttctggt aaaactatag aacctccgat aaatgcaaaa ggtactgaaa ttaatagagg
     9061 aaatgataat cttagtgaca aatgacctgc ttttagataa ttaaaaaagg ctgcagaaga
     9121 tacaataatg tttaatgcta aagagattgg tgggactgta tacaaattga ctccaacaag
     9181 aactaagata gctatataac tacttcctcc agcaaatcct acagaagaat aaaaaaaact
     9241 tattataaag aagataggag caagtattat tagtaaaatg tcattaatga cctttccctc
     9301 ctaacatatt aaaaacatgg aatatgctag gcaaaaggag atctaacgat tctgatactg
     9361 ctttagtgct tcctggcaaa tttacaatta aggtttcccc tcttgtgcca gacattcccc
     9421 ttgaaagcat ggagaatggt gttctccttt gaccatatga tcttatggct tcagaaattc
     9481 catttgcctc tttatcaata accattttag ttgcctctgg agttacatct cttttactaa
     9541 aaccagtacc tcctgaagtt attattagat ctactttttt ttcatctgaa tatttgataa
     9601 gatacgcctt aattttgtct acactatcag gcaatatttc ataacctacg atttcgatat
     9661 tcttagtgat atcaacattt tccaatctat taacgatcat tttaccagat ttgtcaactc
     9721 ttgttccatt aaaagtagag tcacttataa caaatatagc aatggtaatt tttttatcgg
     9781 ggctttcaat aaaatctctt ttacctccag ttttatctaa taactttatc gactctatta
     9841 ttagatgatc gtctattggt ttgagcatat catatatggt aagtgccgct gtgcaacatc
     9901 cggttaatgc ttccatttca actcctgttt tccagacact tttaacacca actttaattt
     9961 ctatagacga ttcatataac aatatttgtg tagaaatgtg atctattgga atagaatggc
    10021 aataaggtat taattcccat gttttttttg ctccaagagc agctgaaatt tttgctgctt
    10081 ctataatatc tccctttggt gttcttttat ttttaatcag atctatacta gcaggagata
    10141 ctttgattat tgcttgtgca gtagcaattc ttaatgtgtc tctttttctg gatacatcaa
    10201 acattccgtt ataaggattt atttcattca cttatcttaa caccttttat ccattcattg
    10261 ccaaattgag ttttttcttt tttccaaatt ggacatcttt ttttaatatt ctcaattcca
    10321 taactacacg cttcaaatgc ctcttttcta tgttctgaag aaactccaac tacaacactt
    10381 atttctccaa cctgaagata tccggttcta tgtactgcaa taaatttctt aatattccaa
    10441 tttttgaaaa cctctctttc gatttttttt aatatttctt caagcatttc atcatatgcc
    10501 tcataaaaaa taccttctac tttccccttg gaattatgat ctctaacaaa tcctataaaa
    10561 gaaactattg caccacaaga atcatcttta atttgatcca atatttttat ttgatcaatt
    10621 gtatcttttg ttattgaaat catataattt cctaccatta tcctccactt attggcggta
    10681 taatagcaat ttcatctgat ggttttacat taatatcttg gttatcttcg ttacgtatta
    10741 ctttattaac agcaaataca gaatttatct cattcaaacc cagagaagga tataactctt
    10801 caagatattt tctaagttct ttcaaggaaa ttgatgtatc atgtatcgaa atgatcaaat
    10861 tatcgctatc cattttctct ctcaaaattc caaataatct tatcttcaaa gtagtatact
    10921 ctgatatatt cacaggtctc aaccacctat tgtatgcata agatttaagc ttggtcgaag
    10981 tccattaatt ctaattaatt gtactatccc ctctggtttc tttttatgac aacttacaat
    11041 ataatccata atttcttgat cagtctttgc atttcgaatc attttcttta agtcatagcc
    11101 ttctttttca aaaagacaag tgaggaatct tccatcagaa gtcaatcgta ccctatcaca
    11161 gtttgaacaa aaaggttctg taattgatga aataaatcca atttttcctt ttccatcatt
    11221 aaaattataa atcgttgcag gatcagattg aaaagaagaa ttattatttt catcaaaaga
    11281 aataacatta tgaaaattag attttatttt ttttattatt tcctgcttag ttacaacaag
    11341 attatgattc caaatacctg atccatcaag aggcataaat tcaataaaac ggacacttaa
    11401 tctagtatct tgggcaaact ttacgaaatt aattacttca tcatcattcc aatttctaat
    11461 tattactgta ttaatcttta tttttaatcc taactcactt gctttttgaa tacctctgag
    11521 aactttattt aaattagaaa taccatttaa agttctaaat cgttcttctc taaaggtatc
    11581 aagactaata tttagagtat ccaatcctgc tgattttaat tgactaatct tatcctctaa
    11641 taaaatccca ttagtagtca tactgatggt tttgatattt tctatctttg ttaaatccct
    11701 aatcaggttc tctaatgacg gtctaagtaa tggctctcca ccagtaagtc gaattttcgt
    11761 tattcctaat tgagaaaaaa ttgctgctat tctaactatt tcttgaaaat taaggatttc
    11821 cttattatcg aaccatttaa tgtttccatt tggcatgcaa taaatgcatt tcatattaca
    11881 cttatcggtt acagatattc ttaactttct tgctactcta ttaaaattat ctttcaaaat
    11941 ttgaggagag ttattttgaa tcaattcttt tcattttata caatttttta tttattaact
    12001 gagaaagttt ttcaagaaaa gattgtttcc agcattttgg gagctcttaa actacttttt
    12061 ccataagtaa atttattatc tttatattct gtaatgagtt caactatatt ttctattgta
    12121 acgtcattat tgttgttgtc ttttttaatt agtttgatag aatcatcagt acctatttcg
    12181 ccctccttta caactttaaa gtaaattcca gatttatccc cctctaaaat tttttgtagt
    12241 atgtttattc taccaaactt gactccaagt ttgtaacaag gcgtacgcgg ttgggttgca
    12301 acctcttgtc gagattctat ttgcaacata tcttcaatgt ttacttgaga tttgaaaaga
    12361 ccaactatag ttaatttttt taaaaacatg ccccagttaa atagtatatc tggaaacttc
    12421 tttttcatag gaaatatcca tcatgaaaga atataagcat aaggtatgaa aaatctattt
    12481 ttcctgattg ttttgcatag aaatgtgctt tgtatcctat cctctttagt cgtataaata
    12541 gaatgattcc attcttaact gataatatag attttagact tttcattata gataatactt
    12601 tccaaatgtt tatattttct ttattgttat tttgaatcgc tatataaatg gattcaattg
    12661 aatactatag caatagtaaa agaagatttt gaaatgttcg acaaagataa aaataaaaat
    12721 tcaaaaaaag cagtagcatc tttgaaggaa aaaaaccata tccatattct tgatttggat
    12781 gatggagaat ttgcatttat ggaatggcta aattcagaaa ataaaaattc tgccgccaat
    12841 catattatac ttaaaagata tgaaaaaggt aaagaatgta ttttaaacga taatacctta
    12901 cactcttata taaaagtaaa aatgaatgat aatgctccat tttgtaccta ttgtcaagat
    12961 gatagttgtg cacatgtagg atttacaata tgcgtaatgc aattatttga aagaaaggag
    13021 attttgttag gggaagatga gaatatgtta gatttactaa aagattaaag atatttatta
    13081 ctagcaattt agtttgaaat gcaattgtaa gatcggatct acttcatctt tttgtaacga
    13141 aaagtgatat agtctcccac cttagatggt aatatacaag ttcctaaact tataatgata
    13201 ggcaatgatc aattttcgtg aagttatttg aaaaaaatct gtaaattgta agggtcatag
    13261 taaaaactta gtagccttat atattataag caatatttat tgtaaataaa atggaattta
    13321 ctgattaaat tcaataaaat atttatttta agtaatagtg ttcatatatt ttatactact
    13381 ttttttaagt aagaatgttc atatcttctt gttgtcatct aaccaaatcc taaataataa
    13441 aaaatctcgg aaaccaaaga agactgaaaa agagttccca aaaaaaggag ataacaaaac
    13501 aagagaagag caaaaatcac ataggaaatt accagaacct ataatatacc aaggagagaa
    13561 tcgaagaaat atattattag cagaggatga tgatgagttt tttaaacaac atagagaaca
    13621 gacgatgagg gaggcaagag caattacaaa ctattttcta gaacttcaca aggatatgat
    13681 aaattcctat aactctgaat attctaactt attacaaaat atatttaatt catcttggga
    13741 tgattttact attccgggaa gatttacaga atatccatct gacataaaaa atacgtattc
    13801 cattttaagt agtaatagag ataactcact gaaattgatt gacaatatta tgacccaaaa
    13861 tttggataca tttatccaat caatcgaact tacacaaaga ttctacaaag atgtaattga
    13921 gagttacttg aattgcataa aaaaataaat agtaatggtc tatgctcctg tcttaaaatc
    13981 tattaaacat actaacagaa tgagaagaaa tatgattaca ttagatgttg tattttattc
    14041 agattcattc tagattaatg aaacaaattg acctaataaa taagaagaat tattaaatat
    14101 cttcagatat aaaaagttaa taccactata ttatatcttt ttactcatta ctttaatcga
    14161 tttttaattt ttattgtaaa tggtcatatt aatactatgg ataatatatc aaagtataaa
    14221 gcaaatgtga ttcttcatta tttccttctt tttgtacgcg gacatctgat ttaaatctct
    14281 aatatcctct ttccaattaa tgtttccact tttttataac taaattgtgt caaatagttt
    14341 agttcttctc tttattaata ttcattactt aatattcttt tatatttctt cttttaacaa
    14401 attaatttga actatcatat tcttaaggct gttaaggtga caaaagaata aagagaacaa
    14461 caaggaatga aataacatat ttttaaaata acaatgtttc ataaaataaa atagaagtat
    14521 ctaagtttaa tattccacaa atttgttgtc atgtattatc atcaactaag ttaagtataa
    14581 tatagtaaaa caattataat tatcataatg aaatcaacaa tcaatttaat cattgtagca
    14641 atgttatttg ttggtcctat tgtaggttca ttttcaatct atggacaaga aacaacaaca
    14701 aataatgtca aagatcaaat tcaaactaca gcagaacaaa taaagaatat ggcagatgaa
    14761 gttgcaaata attctacagg tacaatcaat caaacagagg ctaaaagtat tctaatgcaa
    14821 ttaggagaag cagcaaggaa aacagcatta gccggtgcag acatactttc taatcttagt
    14881 ggagaaatta aagaaggact acgaaattaa ttgcaaacaa tacagtatca acgcctctat
    14941 ttcttgattt ttatatgtct tatctgtaga atgattatct gtatatattc tctctaaccc
    15001 atttagaatt tatttttcgt gtgcatacta ttgtcataat tcacagaatg agtattattg
    15061 atgtgaataa acattcacca tataatgtac agaactgaat tcaaaaaatt tggattttat
    15121 ttaatataac tattataact tatttcatac agatatttta ttacaaatag aaataagagt
    15181 acttattata ctttgtatat tctgtcaaaa tagatttaaa gtactgtatt tttcagcatg
    15241 gtattactaa taaaaatagc aaacgaacaa taataaagat tgtttggttt gctaaattat
    15301 taattattta tttatattac aaactatgat agtttgattt tctacggtta tttaattatt
    15361 atatattatg tctatttaaa acctatatga aatcaaaaat ctgttccata gtaatatcat
    15421 ccattcttgc atttgcttta attgcaggaa tactatcaac atttgtaaat acggtagcac
    15481 aagaagatgg tagagataca gaactaggtg gagacccaga actaggtgga gacacaggaa
    15541 tgaccgaaag tgatctggga ggaaatgaca caggaatgtc aataagtgat ctagaaaatg
    15601 atacagaaat taccaatggt caaatggata atgaataact aggttaattt tgttctatta
    15661 gtagtaaatt ctgttaccta ccaaataata gattggctat gatataatga caagatcatc
    15721 gacgaaattc attctaatag aattaattag ataaatattt catataattt gtttcttcag
    15781 aatgttgtat tcttttactt aaaccaagtg atggacagat agatattttg atttttcttt
    15841 atattatttt ttcaatatat tatgagtttt aactttttat ttcttagagt tttaaacact
    15901 taagaatgca cttaattata ttaaaaagtt aatattaatg atctttttaa tatactataa
    15961 aattcgtgtg atgttaaaat atggaatgac agctatatga ctttattaat actatatgat
    16021 ataaagctta gtcaattatt atataatcct acttgatttt aatctttaac attagacctt
    16081 aatgtattac ttattacagt ttttcttatt ttattaagat ataattcctt tcatcaatct
    16141 atgattatta tataggaaag gaatgtataa gttgatttta attatttatt tagttatttg
    16201 ttattactta tggatagata acactcatta atatcaaaat gtttgataaa aataattata
    16261 tggaaattga gattattgtc gtatattttc cattatttta gtagcctcag tataactaaa
    16321 ggcttttaaa gttacagtac gtgcattacc ttgacttcca atagataata gacaagacat
    16381 taatgtttca tcattaggag cctctactac tgatacccca tcatatttac caaatgtata
    16441 atatgtatca attagtctac cacctgtatt ttctaattta gatttaaaag tattaactcg
    16501 ttttggtgat tcttttatat tttttattcc ctgatcggta taattccaga gaattatata
    16561 gaaagacatt aaatgtagat aaaaaacata aaatataacc ttatcgttca gagaataaaa
    16621 caaataacct ctaagtttaa tattcattcc aatagatgat gaaataaaat ttaaaatatt
    16681 gaaaaatatt tgaaattaag attaaaagaa taatcgataa aagaaacaaa ttactgatac
    16741 aaataaccaa ttcccaggta actatattaa attatcaaat aatatatcta atacaaagta
    16801 ataacatata cccaaatact tgaaaatcta agttatatag tattaattat aaattcgaga
    16861 tgtatcattc attctttcaa tcaaactctt ttccagtggt gaatgccgtt tatgttttaa
    16921 atgtaggaaa tgacatggtt tggataccac tgttccatcc tctgaataaa ctgtatgcag
    16981 aatacttttc aaccaactat tccaaaaaat gtgacaaaac aagcattaca catacttgaa
    17041 acatgaatac caagtataga ttattaattg ttatagaaaa agaaatcgac tatagtgaaa
    17101 ttactgctat aataaattaa tcattgacta atcaatctag agatattaaa tttactgaat
    17161 cttattaaaa accaattact agaaagagac tagaattgaa atagaagtgg atattgatag
    17221 agtagtaggt aaaagtatac aaacttcata tccaatgtaa caatatcaat ttcgaaaatt
    17281 tcattactta agatcttgta attataaagt aaataggtta acatcagttt ccagaagtag
    17341 aaagttaaca aaactacaat agaaaacctt ataataatat tttttgacaa acaatcattc
    17401 atttgaatga gcacaaatca tcacttttag gatccaagat atctagaaca ggagacattt
    17461 tgagaaaaac agccttggac caaatagatg agtatcaact aaaattctca aaggttactg
    17521 atttctataa ttatctattt aaggatttaa tagttgataa atacgatttt acaaaggcat
    17581 cttacaccgc taaaaatttt tttgacaaag atactcttca ttttgttgca atagatggaa
    17641 cagagtattc taaacctttg tttgatatgg taattttttt tgcaggcgct tactcttgta
    17701 atggctctat tgagttttca gagcaagaaa aagacaaagt gaaaataaaa tatcaagatc
    17761 gatttattga ccaatctcta gatatttcta gttgtattcc tgtttatatt gacaaaatcc
    17821 ctgaaataga tcatactttt catgataaag atggaaaggt aaatttgatg aaacctatga
    17881 cggaagagac cataatgaac aatacaaata tagccaattt tctcatgacc ttttcagaat
    17941 tttatctatc ctataaatac gcatctacta aaaagtatga cattatattc atggatagaa
    18001 gtttatcaaa tatgtattct agtttgttgt acga
//
LOCUS       DASVEV010000329         8361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1745498, whole genome shotgun sequence.
ACCESSION   DASVEV010000329 DASVEV010000000
VERSION     DASVEV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745498"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VF222_14240"
     CDS             <1..1051
                     /locus_tag="VF222_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7143634.1"
                     /translation="VELLKKDGISAYPITFKQSDDKCGSAAEHAKELSKIIGQIKDET
                     GQNKVNIVGHSKGGLDARVYLANNTKDVANLVMIGTPNAGSPLAQSSEVCTPAVYDLR
                     PGAAATEVKMNPNTKYYTIAGEWDPKLGNCQLSMFAPMEQSGSSTLPKPNDGVVPLSS
                     VESQEDFINLGHSKSCHTNLMSDYEYGLAKDVIVQGSSNQTGSQQSQQPSQQHQQQEK
                     QKGSQQQKIQNSGIALDLTTPTASNLYGGDKKGSFDFSVQNNNMIGTAEMNEQPADGK
                     VYEGWFEDKGDASGYSLSVGKFNEDENTLAVNQTMVNPYTYTVFFVTAEPVDDPDPNP
                     SDVVVGTKLPIPFGQ"
     gene            1214..1348
                     /locus_tag="VF222_14245"
     CDS             1214..1348
                     /locus_tag="VF222_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143635.1"
                     /translation="MSNKVYVTTTSKYQDSACEPTKIIEHVIELFQNNKKNISNKLLE
                     "
     gene            1834..2070
                     /locus_tag="VF222_14250"
     CDS             1834..2070
                     /locus_tag="VF222_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143636.1"
                     /translation="MSRRRLWQAEDFFLDRNSDPVDNKLIIFASYNKSNSNLSINPFD
                     NFTNRLAKPPMNSNSPIKIANNINERIFYFQLAS"
     gene            complement(2325..2549)
                     /locus_tag="VF222_14255"
     CDS             complement(2325..2549)
                     /locus_tag="VF222_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143637.1"
                     /translation="MLIKLVQVYIPMKIFASRSDRENFIFLIGLIVIIVGILALLLAY
                     VFFPIQPSNNNIGQTAVPDFFIWKNYQILN"
     gene            complement(2990..3310)
                     /locus_tag="VF222_14260"
     CDS             complement(2990..3310)
                     /locus_tag="VF222_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143638.1"
                     /translation="MSTSIDWTELVKDRKGVRTKDNNPCGNIIGNDEANIIIEDGAMR
                     QRIYRVPKLAIPSYNGAELKLNISYDELKSYEVKDDDRGMLESITDSVKDKTKSLKEK
                     TMTK"
     gene            complement(3816..4211)
                     /locus_tag="VF222_14265"
     CDS             complement(3816..4211)
                     /locus_tag="VF222_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143639.1"
                     /translation="MPQKKGWSTIQIREDVKNNLLELYNNDKKRPSNQKFTAYLDLIL
                     KNTIAFNKQIIEYGPFLQFNSAIDNHIAVKDNTMNRLVTIYVNSKKKELQCDFCEKTD
                     CLHIGFCFAVPEIYKVLIENGFRPKKGRR"
     gene            4519..5034
                     /locus_tag="VF222_14270"
     CDS             4519..5034
                     /locus_tag="VF222_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143640.1"
                     /translation="MFYKFKKTLVSAHDYLLKSFKFVIEQNHFIDLTIDSPIIVNLYN
                     LIPKLTIKALLPRNYEDMESPTVTIIDGGIRGQSNNLDFYSFVDYVVQYGVNVNQELE
                     IIVTKSFTAHQLADTIICKLPKMIQKYRSNIVIIMDLFATDEQLHLSEKQWLPGHMKS
                     IQKLKITNKKQ"
     gene            5042..5314
                     /locus_tag="VF222_14275"
     CDS             5042..5314
                     /locus_tag="VF222_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143641.1"
                     /translation="MGRIIPSFRIATLMEENRWKLYRKYLNKREKKIFNNMFSFAYLY
                     NSASSYASNPVIIIPIIVSIALHHHIKISLFKKSTIIIFFSIINIE"
     gene            5319..5597
                     /locus_tag="VF222_14280"
     CDS             5319..5597
                     /locus_tag="VF222_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143642.1"
                     /translation="MKDKIYSSNLFPYSDIIIKEIESWKSYADCLRKQDRKLFYEMLN
                     SCYKYSSAITVKGKEYSTTSVIISILLKHHKQLALETKEDEWYHKGDE"
     gene            5657..7909
                     /locus_tag="VF222_14285"
     CDS             5657..7909
                     /locus_tag="VF222_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX7143643.1"
                     /translation="MIFWIRIKDSANDKKKYIRIEDEWQNYFYLASNDISKLKEIIQN
                     DGISSSIISHEFVKRFEKITDTKKKIVLKLFTAGRNLKLTNTIEKMIEYHDFRLYNVD
                     QLPEQQYFFDHDIFPLGFFRINNNTSGLDWKTKGETVWSTDYYMPYFEKIYIKLNFPK
                     GKIVKLTDELNSISIIHYNDDNNSNNKDKSQIIDITESSEKRKLQELIKLVQDIDPDF
                     IFTDDGDSFTFPYLIYRARINGINLCLGRDNNISLTTPKRKGNSFFSYGRTYFKPSTI
                     KLFGRIHIDKSNSFTIRTGSDLEGLFEISRLCRIPLHEASRASIGKCLTSLHLYNATK
                     KEILIPWKPILNEHPKNLLELFLADRGGLILEPEIGLHEKIAEFDFVSLYPNIMLKRN
                     VSAETILCDCCFDNPKFRVPELNYHICNKNGLIPESLEIVLDKRLRYKYLKNKSTNLL
                     LKSIYDKRQSALKWILVTSFGYLGFSNAKFGRIDAHIAVCAFARNILSQTMHIAEDMN
                     FDVLHGIVDSIWVKKDNATKKSYSKLKETIEKETEFDISFEGVYKWIVFLPSEINSHL
                     PVLNKYFGVFEDGTIKTRGIEGRRHDTPKFLIQCQNEILEILAQGNSISDIKQNKLSL
                     IIKKIENYLSLLKNRKVEPEDLVFTKILSKNYNHYTNRNTLENSAIKQLELNGDYLKA
                     GQILQYIVTKYSESLLKRVVPIQLVDQRTTYDVKRYSELLIDTCNRILKPFGIIFDQT
                     QSVYRLDNYY"
BASE COUNT         3197 a         1142 c         1207 g         2815 t
ORIGIN      
        1 ggtagaactt ctcaaaaaag atggcatctc tgcctatcct ataacattca aacaatcaga
       61 tgataaatgt gggtcagctg cagaacatgc aaaagaatta tcaaaaataa ttggacaaat
      121 caaagacgaa acaggacaaa ataaagtgaa tatagttgga cacagcaagg gaggattaga
      181 tgcaagagta tacttggcta acaatactaa ggatgttgca aatcttgtca tgattggaac
      241 accaaatgca ggctcgccct tagcacagtc aagcgaagta tgtactcccg ctgtatatga
      301 tttaagacct ggggcagcag ctactgaggt aaaaatgaat ccaaatacca aatactatac
      361 tattgcaggt gaatgggatc ctaaattagg aaactgtcaa ttatcaatgt tcgcacctat
      421 ggaacaatca ggttcttcta ctcttccaaa gcctaatgat ggcgtggtac ctctatcaag
      481 cgtagaatca caagaggatt ttattaatct tggacatagc aaaagttgcc atactaatct
      541 aatgagcgat tatgaatatg gattagcaaa agatgtaatt gttcaaggta gtagtaatca
      601 aacaggttct caacaatcac aacaaccttc acaacaacat caacaacaag aaaaacagaa
      661 aggttcccag caacaaaaaa ttcagaattc aggtatagca ttagacttga ctacacctac
      721 tgctagtaat ttgtatggtg gagacaaaaa aggtagcttt gacttttcag tgcaaaataa
      781 taatatgatt ggtactgccg aaatgaatga acaaccagcc gatgggaagg tatacgaagg
      841 ctggtttgaa gacaaaggag acgcatccgg gtattcttta agcgtaggta aattcaatga
      901 agatgaaaac actctagctg taaatcaaac aatggttaat ccttacacat atacagtatt
      961 ttttgttact gctgaacctg ttgatgatcc tgaccccaat ccctctgatg tggtagtggg
     1021 aaccaaattg cccattccat ttgggcaata accttttttt aatatttttt atatagcatc
     1081 aacaatttgt catttatttt caataccttt gaaacagaga attagaaaaa ttttagaacc
     1141 gcataggcaa tgtgatatga ttcttccttc aaagatatta aacaaaaaaa ccttttaaaa
     1201 aatctgattt gctatgagta acaaagtata cgtgacaact acgtccaagt atcaagatag
     1261 tgcttgcgaa cctacaaaga taatagagca tgttattgaa ctattccaaa ataataaaaa
     1321 gaatatatcg aataaactac tagagtagat taataaagaa ggagaaaacg gatagtaaac
     1381 gattcataga tagatatgca gttagttaga tagatagatt attatgcttt cgctgatttg
     1441 atttggatag atggtgtgta aggggatatg ttcacgccat aaagcaatta ggccttcaac
     1501 tggtcagcgt tatttgatag gacaaaaacg ctgtcaagtt tgtcaaattt tcatcaattg
     1561 gagaggtaca ttttgccctt gttgtggtta taaactaagg gttacgccaa gaaataaaaa
     1621 attcaaacaa attttaagaa gtattaaaaa ttaggccagt aatcattgga ctattaacga
     1681 tattagcagt actgtttggt atatctaaga taaccggcct atacgttaag gggcttctgt
     1741 ataaatgaat ggaattagga ttattacctt aacaataatt tttttaataa tagttaaaag
     1801 ccatatccct tctctaaaag aaagagacaa agaatgagta gaagaagact atggcaggct
     1861 gaagattttt tcctggacag aaattcagac ccggttgaca ataaacttat tatttttgca
     1921 agctacaata aatctaattc aaacttatca ataaatccct ttgataattt cactaataga
     1981 ctagccaaac ctcctatgaa ttctaatagt cctatcaaga ttgccaataa tattaacgaa
     2041 agaatattct acttccaact agctagttaa atctataggt ggaccttact aatagtattt
     2101 tgtaatttat attataatca aaaatatttt atcattaaca atatatttaa atttgttttc
     2161 aaattttaag gcacttcaaa atctattatt ctctattatg aggaacttaa gatgtatcgt
     2221 tgtagaagaa gctagggaag tagtaaagaa tcattagact tgctagagaa aaacaattcg
     2281 aagagtttgt cagagtaagt tttagaattg ataattttat ggaattaatt taagatttga
     2341 tagtttttcc atataaagaa gtcagggact gctgtttgtc caatattgtt gttagatggt
     2401 tggattggaa agaaaacata agcaagtagc aaagctaata tgccaacaat aattactatt
     2461 aaaccaataa gaaaaataaa gttttctcta tcagacctac tagcaaaaat tttcataggt
     2521 atgtatactt gaaccaattt aattaacatt gtcctcaaaa ctttatatta ataattacac
     2581 aacataacaa atctttgaat tatatttata ctcaaaatat gatttaagct agaataccaa
     2641 gtggagtata gataatatat tgatgagatc gtattggttc attacctgga tattttccaa
     2701 tacaatgaat gaaagtaatt cacaaaaaca gtgttatcat ttaacaaatc agaattaaat
     2761 gccaaataaa agcaccagca attatattgc taagaccaat ttttacatat atatcctgct
     2821 gctaaagctt agccccctct ctttctctaa agtgtaaatg aaccagatta tatttttttt
     2881 aaatttgata atatttaaaa tgaataataa aaaggaattt tttagactaa taataataat
     2941 ttaaactaaa ataattcact attcatcctc atcctcatca tgtaataatc tatttagtca
     3001 tggttttttc ttttagtgat ttagttttat ctttaactga atctgtaatc gattccaaca
     3061 tacctctatc atcatctttt acctcgtaag attttaattc gtcatatgaa atgtttaatt
     3121 ttaattccgc accattatag cttggtatgg ctaattttgg aaccctgtaa attcgttgac
     3181 gcattgcacc atcctcgatt attatatttg cttcatcatt tccaattata tttccacatg
     3241 ggttattgtc tttagttctg actccttttc tatctttaac taattcagtc cagtcaattg
     3301 aggttgacat aacaaatctt atattttatg atatatagca attttataaa tattaagata
     3361 aattatatta tattcaatcg ttaatcatgt agagcacacg tttacgaatg ttattatttt
     3421 taaaatattc tcatcattaa tcttttttta cttagcgtta tataattatt agaatcttat
     3481 ggctaatcaa atagtaatag atatgacttt tcaataaaat agaactagta acaggtggta
     3541 caagaacttt gggaagttct ataacaaaag gctttccttg agtccaacgc aacggtaata
     3601 tctttatatc ttaatggcag agaaactggt catacacaaa cagataataa atctacatcg
     3661 acagtacaac tagtcaaagc aaatgtaaca aatgaagaag aggtatacaa attagtttct
     3721 aatatctatg acaaatatgt attcattact cgaaaataaa aaaatcttta tgaaaatctt
     3781 tttaatttat ctaaggtaat tttaaattat tatagttatc ttctaccttt ctttggccta
     3841 aaaccattct caattaatac cttataaatt tctggaactg caaaacaaaa cccaatgtgt
     3901 aaacaatcag ttttttcaca aaaatcacat tgcagctcct tcttttttga attgacatat
     3961 atcgtaacaa gtctattcat tgtgttatct ttaacggcta tgtgattatc tatggcagaa
     4021 ttaaattgaa gaaatggacc atattctatt atttgtttat tgaatgctat tgtatttttt
     4081 aaaatgagat ctaaataagc tgtaaatttt tgattagacg gtcttttttt atcgttatta
     4141 tataattcta ataaattgtt tttaacatct tccctaattt gaatagtaga ccagcctttt
     4201 ttttgtggca tttttatata agttagatta ggttaaagta tgatataatc tttattttta
     4261 ttaaaatatt gtaaagtaaa tagaatttta ggttaaatta acataaactt taagtactat
     4321 agttaggtta aattaggtta atgactaaag atttaataat cagaattgca tataatttgt
     4381 ttaaaaaaat aaaagaaaat aacaaattta atacaattat ttacagaaat ataataatag
     4441 tatatttgct tctatatatc ataattataa aatacatata caattctatt atcaaacaat
     4501 ataggagttt cttttaaaat gttttataag tttaaaaaga cacttgtatc agctcatgat
     4561 tatttattaa aatcattcaa atttgttata gagcaaaatc attttattga tttaacaatt
     4621 gattcaccta ttattgtaaa tctttataat ttgattccta aactgactat caaagcatta
     4681 ttgccaagga attatgaaga tatggaatct cctactgtaa caattattga tggaggtatt
     4741 agaggtcaat ccaataattt agacttttac agctttgtag actatgtagt tcagtatggg
     4801 gtgaacgtta accaagaatt agaaattata gtaacaaaat cttttactgc tcatcaactt
     4861 gcagatacaa taatttgtaa attgcctaaa atgattcaaa aatatagatc aaatattgta
     4921 ataattatgg atttatttgc taccgatgag caattacatc tttctgaaaa acaatggctt
     4981 ccaggtcaca tgaagtctat acaaaaatta aaaataacaa ataaaaaaca gtgatgaaat
     5041 aatgggaaga ataattccat cattcagaat agctacatta atggaagaaa atagatggaa
     5101 gctttataga aaatatttga ataaaagaga gaaaaaaatt ttcaataata tgttttcatt
     5161 tgcatatctt tataattctg catcatcata tgcttccaat ccagtaataa ttatcccaat
     5221 aatagtttca attgccttac atcatcacat aaagatatca ttgtttaaaa aaagcactat
     5281 aataattttt ttttcaatca tcaatattga gtaaatatat gaaagacaaa atatattctt
     5341 ccaatctttt cccatatagt gatattatta ttaaggaaat tgagtcatgg aaaagttatg
     5401 cagattgtct aagaaaacag gatagaaaat tgttttatga aatgcttaat agctgttaca
     5461 aatattcctc agcaattact gtaaaaggaa aagaatattc tactacatct gttataatat
     5521 caattttact gaagcatcat aaacaacttg ctttagaaac aaaagaagat gaatggtatc
     5581 ataaaggaga tgaataagaa aaattactat atctggttgg ctgtttgatg cttattcatc
     5641 cgccgatgga agtaaaatga tcttctggat caggataaaa gattctgcaa atgataagaa
     5701 gaaatatatc cgaattgaag atgaatggca aaattatttt tatcttgcat cgaatgatat
     5761 atcaaaacta aaagaaataa ttcaaaatga tggtatttca tcttctatta tctcacatga
     5821 atttgttaaa cgattcgaaa aaataacaga tacaaaaaaa aaaattgttc taaaactttt
     5881 tactgctggc agaaacttaa agttaacaaa taccattgaa aaaatgattg aataccacga
     5941 ttttagatta tacaatgttg atcaattacc tgaacaacag tatttttttg accatgacat
     6001 ttttccctta gggtttttta gaataaataa caatacttca ggtttagact ggaaaactaa
     6061 aggtgaaact gtttggtcta ccgattatta tatgccatat tttgagaaga tatatatcaa
     6121 gctcaacttt ccaaaaggta aaattgtaaa attaactgat gaattaaact ctattagcat
     6181 tattcattat aatgatgata ataatagcaa taacaaagat aaaagtcaaa ttatagatat
     6241 tacggaaagc tcggaaaaga ggaaattaca agaattaata aaacttgttc aagacataga
     6301 ccccgatttt atattcactg acgatggtga ttcatttaca tttccatatc ttatttatag
     6361 ggcaagaata aatggaatta atttatgtct gggtagagac aacaacatat cattaacaac
     6421 tcctaaaaga aaaggaaatt cttttttttc ttatggtaga acttatttta aaccatccac
     6481 tatcaaatta tttggaagaa tccatattga taaaagcaat tcatttacaa ttagaacagg
     6541 ttcagattta gaaggtttat ttgagatttc aagattgtgc agaatcccat tgcatgaagc
     6601 atcaagagct tcaattggga aatgtttgac cagtctgcat ctttataatg caacaaaaaa
     6661 ggaaatattg attccttgga aacccatttt aaatgaacac cctaaaaatt tgcttgaact
     6721 tttcttagct gatagaggtg gcctgatatt agaaccagaa ataggcttac atgaaaaaat
     6781 agctgaattt gattttgtat ctctttatcc aaatattatg ttaaaaagaa atgtttcggc
     6841 agagactata ctatgtgact gttgttttga taatcctaaa tttagagtac cagagttaaa
     6901 ctatcatatt tgcaacaaaa atggattaat tccagaatca ttagaaatag tattagataa
     6961 acgattaaga tataagtatt taaaaaataa atctacaaac ctattactaa aaagtattta
     7021 tgacaaaaga caatcggctc taaaatggat acttgtaacc tcttttggat atcttgggtt
     7081 tagtaatgca aagtttggaa ggatagatgc tcatattgct gtttgtgcat ttgcaagaaa
     7141 tattctttct caaacgatgc atattgctga agacatgaat tttgacgtct tacatggaat
     7201 agtcgattca atttgggtaa aaaaagataa tgcaacaaaa aagagttatt ccaaattaaa
     7261 agaaaccata gaaaaagaaa ctgaatttga tatctctttt gaaggagtat acaaatggat
     7321 tgtatttctt ccatctgaga ttaattccca tcttccagta ctaaataaat actttggagt
     7381 ttttgaagat ggaactataa aaacaagagg aatagaagga agaagacatg atacaccaaa
     7441 atttttgata caatgtcaaa atgaaatttt agaaatcttg gctcaaggta actcaatttc
     7501 agacataaaa caaaataaat tatctttaat aatcaaaaaa attgagaatt atctttctct
     7561 attgaaaaat cgtaaagtag aaccggaaga tcttgtattt actaagattc tttcaaaaaa
     7621 ttacaatcat tacactaaca gaaatacatt agagaatagt gctattaaac aacttgaact
     7681 caatggagac tatctaaaag ctggacaaat tttacaatat attgtaacaa aatatagtga
     7741 gtctttattg aaacgggtgg ttcctattca attagtagat cagagaacta cttacgatgt
     7801 taaaagatat tctgaattat taatcgatac ctgtaataga atattaaaac cattcgggat
     7861 aatattcgat caaactcaat cagtatatag attagataat tattattaat tctaatacac
     7921 attttcatat ttgaactttt atattctatt gtattacttg tttttgataa ctctttttta
     7981 tatatgttca agataatgtt tctcggttaa ttttcagcta ataagtttta tgaaaataaa
     8041 taaataaaaa gatattatta ctaaataaaa aatttattat tgctatcatt tattaaagct
     8101 atatgaccga tttttggaat agttattagt ccattgtata aggacatatt ctttgtagaa
     8161 ggtgaataaa taccagttca attgtagaaa ataaaacaac aaatgaaata ataaaagata
     8221 tgataaataa aatattgttt ttatgattac tgctattacc atattctttt ataattggca
     8281 tatatattta gtagtgtatg gatttagtat ttaaaaaatg gtaaaccaag ttaattaata
     8341 attttagtta actttttttt t
//
LOCUS       DASVEV010000330         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_1162692, whole genome shotgun sequence.
ACCESSION   DASVEV010000330 DASVEV010000000
VERSION     DASVEV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162692"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF222_14290"
     CDS             complement(<1..287)
                     /locus_tag="VF222_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143644.1"
                     /translation="MSIRKNHKFILKKDRTNTKTIILVIFPQVKGTLIEQKFKNNEKF
                     LFSINGSIIDESTYQIQRIDQIINNKRQYKLPNLGNIILSRSNLTFNISNLP"
     gene            complement(277..1263)
                     /locus_tag="VF222_14295"
     CDS             complement(277..1263)
                     /locus_tag="VF222_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX7143645.1"
                     /translation="MIPKKVIIAEDTINDIVEESNRHENVETGGLLFGKIIGECIIIL
                     KIIHSSTNAKRTQVYFEMDEDFAINITKQMEKNNLAYLGNWHKHLGYGGPSNGDDKQA
                     ELFLIQNSHKRNYLSLIIDFYNNDYNLIATNYYFDEDEFIKKDIQIQPIINIQELNKI
                     FKRKKFNIRNFIDHLSKNLEDHTGKTFIHSQSNKLQSYKLFYEEIYDLQNNESKTKNK
                     IVYSVFIKFPYLNMSMADKISIGVTFDRKFTRIVEEIQFSDIFFNIVELLPKIKNMFD
                     KSQYYIKILLHSDNKKENSDFKNRIKIIKVIFRALKSIYIQHSSKAKAKNVN"
     gene            complement(1253..1411)
                     /locus_tag="VF222_14300"
     CDS             complement(1253..1411)
                     /locus_tag="VF222_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143646.1"
                     /translation="MPRDLKHVIEGLENIKNKAAEVVIRRKEEDNMVDIETRKVTIPN
                     IKHFFNDS"
BASE COUNT          771 a          310 c          262 g          901 t
ORIGIN      
        1 ggaaggtttg aaatattaaa agttaaattt gacctagata gtataatatt acctagatta
       61 ggtaacttat attgtctctt attgttgata atttgatcaa ttcgctgtat ttgatatgtt
      121 gattcatcaa tgatagaccc attaatggaa aatagaaatt tttcattatt tttaaatttc
      181 tgttctatta atgttccttt aacttgagga aatattacaa gaattatagt ttttgtattt
      241 gttctatctt tttttaatat gaatttatgg ttttttctaa ttgacatttt tagctttcgc
      301 cttagatgaa tgttgaatat agatagattt taaagctcta aatataactt ttataatttt
      361 tattctattt ttaaagtcac tgttttcttt cttattatct gaatgtaaaa gtatctttat
      421 ataatactgt gatttatcaa acatattttt tattttaggt aataattcta ctatattaaa
      481 aaaaatatct gaaaattgaa tttcttcaac tattctagta aatttacgat caaaggtaac
      541 tccaatacta atcttatcag ccatagacat attgagatat ggaaacttga taaaaacaga
      601 atagacaatt ttattttttg tcttggactc attattctgt agatcgtaaa tttcctcata
      661 aaataattta taactttgta atttattcga ttgtgaatgg atgaatgttt taccggtatg
      721 atcttctaag tttttcgata gatggtcaat gaaatttcta atgttgaatt tttttctttt
      781 gaaaatttta tttaattctt gtatattaat tatcggctga atttgaatat ctttttttat
      841 aaattcatct tcatcaaaat agtaattagt tgctataaga ttataatcat tattataaaa
      901 atctataatt aaggacagat aatttcgttt atgactattc tgtataagaa ataactcagc
      961 ttgtttatca tcgccattac ttggtcctcc atatcctaaa tgtttatgcc aatttcccag
     1021 ataagcaaga ttattttttt ccatttgttt tgtaatattt attgcaaaat cctcatccat
     1081 ttcgaaatag acttgtgttc tttttgcatt agttgatgaa tgtattattt ttaagattat
     1141 gatgcattct cctatgatct taccaaaaag taagccacct gtttcaacat tttcgtgtct
     1201 attactttct tcaactatat cattaattgt atcttctgct atgattactt ttttaggaat
     1261 cattaaaaaa atgttttata ttagggatag ttacttttct agtttctatg tcaaccatat
     1321 tatcttcttc ctttcttcta ataactacct ctgcggcttt attttttata ttttctaaac
     1381 cttcaatgac atgtttcaaa tctctaggca ttattaatat atagcaataa gatatattta
     1441 ttactttttt actagttaaa ctctaaaaag catcatagct aataaatacc tcatacattc
     1501 taactaaatt ttcatatcat tgaataaaca atactccaaa tacgttagat taacaggatc
     1561 attataccgc attctcaaac attgcaggat tccatttact tgcatagaaa cagctataac
     1621 atctacacag tatagcaaca catagcacta gttacaataa gatgagtatg aaggcaagag
     1681 ttacagaatg ttacagacta tcttgttgag gcatactctt tgcgactatt tttacaatca
     1741 tccaaaatac ttcactatac aacattatag aaattcacag atagaagcaa catcataata
     1801 cttggcaaaa tagtatcatc attctacaac tttagatttg caatacatat gtttaattat
     1861 tcgatttttg tttcatatta tgagtctttt taaagaatca atatcagagc ggaaaccact
     1921 ggcccaatag tgataatagt tctcaaattg aattcactca aaaataaaaa aataaggtga
     1981 atcaaaaaat aaaatgaata aaacagaaga gttatgagat atcaaaattc atcctcttta
     2041 tttgtatatg cagtcagatc acaagttaca aaagattact atcttaatga taaatctaaa
     2101 aaacctataa ggattttttt ctactggtta tttatttatt tgcattcaga taagtgaata
     2161 aataatatca caatgaatgc gctacaggta agtcatttga tttatcaaaa tgtgtaatat
     2221 tcttattctc gcattctgaa tttt
//
LOCUS       DASVEV010000331        16307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U2071
            k141_776587, whole genome shotgun sequence.
ACCESSION   DASVEV010000331 DASVEV010000000
VERSION     DASVEV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961391
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 16307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:33:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,826
            CDSs (with protein)               :: 2,826
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16307
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776587"
                     /isolate="SMAG_U2071"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF222_14305"
     CDS             <1..535
                     /locus_tag="VF222_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143647.1"
                     /translation="GREVEECTPLEITEILNNAVDDPKEDPFSTIKKQQSVLQNHKKY
                     NKNDREKTSFQQTQPQFNQLETYPITKEDNGLQTNNESKSVIRNIYSQINETLEAIIL
                     DGSMNTVMKVPVSEIVKKLSDSYGGKILVLDGIVTQRLIDASDKAGIEQIIGHRLADI
                     KRNSSIKVGTFSEMGLQ"
     gene            559..1254
                     /locus_tag="VF222_14310"
     CDS             559..1254
                     /locus_tag="VF222_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX7143648.1"
                     /translation="MNTITFQHIVILSELLLCGAKNNYVELTTNEIAKKIKRSQQLAS
                     KELLDLEFFGHISRNKKSKKYTIKVTEKGYQQVYDLFLFLKTAIESFKYIIDFEGHII
                     TGMGEGAYYMSLNGYKQQFKEKLGYEPYPGTLNIKLNSMLYVEAKKEMLKYPSIDIEG
                     FSDQSRTFGWVKCYPAYINDSVDINSSILILERTHYDDSIIEIIAPFSIKEQFSLKNG
                     DYIKLKVNTNYPI"
     gene            complement(1265..1897)
                     /locus_tag="VF222_14315"
     CDS             complement(1265..1897)
                     /locus_tag="VF222_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX7143649.1"
                     /translation="MKSLDKLILKAIYISNLDPSKSFLRYLQEKYQLDKSIFESRMTE
                     LIELGLIGKINPEQYKLNSLGRETMKVVLVGGVFDILHPGHIFTLKAAKLLGDVLIVI
                     IATESTATKIKKNRIIFHNENLRREMVSSLNFVDLALIGKEGTLFDTVEHVKPDIIAL
                     GYDQSHTEKFISENCKKRNINVRILRLNTPIPNMKSSELKRDLGDSLYKI"
     gene            2125..2301
                     /locus_tag="VF222_14320"
     CDS             2125..2301
                     /locus_tag="VF222_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143650.1"
                     /translation="MDPSTRNFICKSCGLYASRDKMEELKDKSQIGSSVSKQRTHDDY
                     LDWWQSSKKEKQRN"
     gene            complement(2309..2809)
                     /locus_tag="VF222_14325"
     CDS             complement(2309..2809)
                     /locus_tag="VF222_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7143651.1"
                     /translation="MNIKPNLLDYINCRILDVLSKNSSLPFVELAKQIGISDATIHTR
                     VKKLQTLGVIKKFTIFVDNDLLGYDHLAFINLKIERGKTEETTKNLMEIDEVLEIHEI
                     YDKFDLLIKIRSKNLDNLRDIIVNKILSSNYVKETELMTVLRTRKEEQMISLQKEMSE
                     KTEEFF"
     gene            3124..3918
                     /locus_tag="VF222_14330"
     CDS             3124..3918
                     /locus_tag="VF222_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HEX7143652.1"
                     /translation="MVNYGDKIAVGVSGGKDSLSLLLVLTHIFERKKTNDIIAITIDE
                     GIAGYRNESLRIVKDFCSKLNVKNEIFSYKELFGMDMDESMLLRPSEKMTSCSICGTF
                     RRRAIDVAADYVGASVIATGHNLDDHLQSFMINIIAGDVNRIGWIYPEPIYYGKNEIK
                     KIKPFIEIYERELVFYALQQNIPFQSEQCPYQDESIRSEIREFFNSLEETRSGVKYNT
                     YNSLLKISKILKNHDSSVKNKCRICGHDSTRSVCSVCKNIKILQND"
     gene            3959..4269
                     /gene="ffs"
                     /locus_tag="VF222_14335"
     ncRNA           3959..4269
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF222_14335"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(4334..5338)
                     /gene="dph2"
                     /locus_tag="VF222_14340"
     CDS             complement(4334..5338)
                     /gene="dph2"
                     /locus_tag="VF222_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HEX7143653.1"
                     /translation="MISIDEDKIFEVIRKTKPLSVALNGPEALIPKMQETGMKIMKRY
                     EIPAYIIGDTCWGSCDVNTHAAEMLGADLLFNIGHTISMETIDKKVIMINAYDDVKFD
                     IVVEKFAKDMANKEYKILGILTDSQHLDQLQPSRKILENHGFKIVIGDGKGQLNDGQV
                     FGCEFYPAHDIQKRVDAYIFLGQSMFHSVSIAMSTEKPTFMLDPYFNEFQQVNDKARI
                     MEKKTILSIYKAQEATKIGIIIGLKEGQFSKIKALKFKKSLEVLGKDIQLIALTEITE
                     ERLRNFKGIDAYIQVACPRIATDNHFSKPLLSVPQAEALIKVLKNEPLDDFLKLRHWL
                     "
     gene            5457..6308
                     /gene="folP"
                     /locus_tag="VF222_14345"
     CDS             5457..6308
                     /gene="folP"
                     /locus_tag="VF222_14345"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7143654.1"
                     /translation="MTKIGDVSINRNSVKIMGIINVSPESFYKNSIKTNLNSISSYVK
                     KLENDGADIIDLGAMSTAPYLDTVISSKIEILRLKKAIMIIRHNCKLPISVDTPRARV
                     AEEAIKIGASAINDVCGLKYDPQMAEIISKHNIPVILGAYEKKPSKILTAKSFSTKRI
                     LHNSITIAQKAGINKTNIIIDPSIGFFRKEGNNPFFTKITTPWYCRDIEIVSNLKELT
                     KLSFPICISLSNKSFLGKLMNLRINERLIPSLIMEVIAVINGATVIRTHNVKETVLAL
                     NTLQLLN"
     gene            6432..7865
                     /gene="guaB"
                     /locus_tag="VF222_14350"
     CDS             6432..7865
                     /gene="guaB"
                     /locus_tag="VF222_14350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7143655.1"
                     /translation="MNIREGLTFDDVLLIPKRSPIISRSQTNLKTRLSRNISLNIPII
                     SANMDTVTESPMAIALAREGGIGIIHRFMTIQDQVDEVLKVKRSESVMIEQPYTISID
                     STVGYAKKIMHDFGISGLLIEKDKKLAGIITKRDLLFETNFENNVSSVMSKDVVFAEL
                     GTTIEKAKDILHKNRIEKLPIIDKDKQIIGLITSKDILKMEEFPNASKDKKGRLLVGA
                     AVGVKGDYLERTEALLDAGADVMVVDIAHGHSDNAINCVHLIKKAFKDCELIAGNIAT
                     GHGTEDLIRAGVDAVKVGVGSGSICITRVITGSGVPQLTAILDSVKIAKEYEVPVISD
                     GGIRNSGDLTKALAAGSSSVMVGSLLGGTDESPGKTLVKNGKKYKIYRGMASFYASLG
                     RKYREEGEQLIESEDLNDYVPEGVEAMVSYKGSVVEIIRQLVGGLRSGLSYCGAKNIN
                     EMQQNAEFVQITTAGYIESQPHDVNVM"
     gene            8033..8257
                     /locus_tag="VF222_14355"
     CDS             8033..8257
                     /locus_tag="VF222_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HEX7143656.1"
                     /translation="MSIDVTVQVLKENVGKTIMIKLKGDKVVKGILNKFDQHMNLVLS
                     DATEYKAGDQTKLGNILLKGDNIIIISPQE"
     gene            complement(8424..8831)
                     /locus_tag="VF222_14360"
     CDS             complement(8424..8831)
                     /locus_tag="VF222_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX7143657.1"
                     /translation="MIKINCKKIVGGKAEGLALLSNKPLNMLSMLDTKSGQINDKDHD
                     LFNQTLKNKILIFPNCIGSSVGAYIFYSLKMNGVSPIAIICTNKCDIITASGCAISDI
                     PLVDGTGSCFKSLNNNTKVIVDADKQMVIIHTV"
     gene            complement(8839..9987)
                     /locus_tag="VF222_14365"
     CDS             complement(8839..9987)
                     /locus_tag="VF222_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX7143658.1"
                     /translation="MELTKEEEKALHGEYGDGLKLAYKILVAIGNANNATKLIPIKWA
                     HISGVNFNTIGEAGVRFLSKLDKETKVTVTTTLNPMGFDSKKDNNISGDFKANQLSII
                     NSYLSLGVKPSFTCIPYEIYDLPKKGEIVSFAESNAAIFVNSILGIKTNKESSLSSLA
                     SAITGKTPYSDLLIDDLRKPTISIKPDIDIKTESDFGLLGYFAGKISKDICVGLEIKS
                     ENLDIIKSKAISAGIGTSGMCGMFTNKNNDCEKISFGKTELRQVKDDLNTAEKGDLIV
                     FGSPQLGLDEVNLLGHLTKGKKFKKPCIIFCPRSVTECNNKMIKKLKKAGADITCDSC
                     TCLTPLITKNEYDSIVTNSIKCAYYMKHSNKIDVSLKDMKSITKEYCE"
     gene            10114..10842
                     /locus_tag="VF222_14370"
     CDS             10114..10842
                     /locus_tag="VF222_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX7143659.1"
                     /translation="MHFVTWFPYYQGIREKMGYSTEEDQKAAYLLSNLIKRKFLDPRV
                     LNKKVKGKRILVIGAGPNLDLYTSFIKKNRSLVKIVADGAVKFLIENNIKPDIVVTDL
                     DGDPRFLIKAEKLGAIIVIHAHSDNIALMEKYVPKFRRIIGTTQVMPVPHVFNFGGFT
                     DGDRCVFLAEEFRAKDIVLVGMLFDDSIGPYSKEIINNVKVKREKLQIAKKLLQVLAK
                     NSKSNLFDTSKKPIIGFKPFKIMP"
     gene            11534..12205
                     /locus_tag="VF222_14375"
     CDS             11534..12205
                     /locus_tag="VF222_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143660.1"
                     /translation="MLNYSEEDIKNAAELRDWVLKQLSDKQEEIDRLKRLLLLIDNLL
                     KQESFKPASKLERSLNNTENSTISERQEPVIRNKSIVKTDYKKHNLNNLNENVSTFTE
                     TRELKRLKDNLLLAKVNISSQTIEIIPEKDLLFSIDTPPFKSFFIKRILEGMITQDRD
                     KNRQKQSLNHELLNYIIDEHEGRIKKIIINNYREKERLTDIINTSAWVFTRMLEKDNT
                     FVNRT"
     gene            12207..13736
                     /gene="guaA"
                     /locus_tag="VF222_14380"
     CDS             12207..13736
                     /gene="guaA"
                     /locus_tag="VF222_14380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7143661.1"
                     /translation="MDKIIILDFGSQYSHLICRRIRDANVFCEIYPYNLKSDQIKELS
                     PNGIIFSGGPASVYSENSPKPDKKIFELGIPILGICYGHQIIVDNFKGKVKRSYAREY
                     GKAILTIDDKSDLFINLDGSINCWMSHGDVAEKTPNGFKILAHTASSFAAAIGNQEKK
                     FYGIQFHPEVVHTERGFEILKNFSINISKAKPEWDMTNFVESTIENIKKKVGNDKILC
                     AVSGGIDSTTVAVLLNHAVHDNLQCIFVNHGLLRYGEESMVMNLLTKIGLKITYIDAS
                     EKFLSKLKGITDPEEKRKIIGHEFINVFQEEVKEKEEFRWLAQGTLYPDVIESGISLG
                     PASIIKTHHNVGGLPTLLNLKVLEPLRHLYKDEVKKVAKILEIPDELLNRHPFPGPGL
                     AVRIIGEITSEKIRISKHASKIVEDELTTENLYNFVWQAYAAVGDDKAVGVIGDERLY
                     GHIVTIRIVNSVDAMTADWTKLPYPLLERISNRITNEVDGVSWVTYAISSKPPATIEP
                     Q"
     gene            14288..14416
                     /locus_tag="VF222_14385"
     CDS             14288..14416
                     /locus_tag="VF222_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143662.1"
                     /translation="MSEESIRCPYCESLFNNNEELSKHIDRIHLGSGLLEGNSRKF"
     gene            14669..15646
                     /locus_tag="VF222_14390"
     CDS             14669..15646
                     /locus_tag="VF222_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143663.1"
                     /translation="MISINGSLISAGKFLIIPDPYQKFASLEIHDNELVDSNKTIGVI
                     SLRGISNNNYTITQKAVNNNYLPNTISKVVEIKDDSPISSVLFVNNFNFKSEKPLSST
                     SNLFTYNAKFVCGSIRGNEGPLRPGHYDTDISIYNKQNYPITLFWNVVFNNYNSSNSI
                     IKTIDPKQSTGLVCKDIRKLAHLNAQDTDLIEGFVIIQIEEMNIASGNKASFANEPVS
                     VQVFYTANALDSLPQETLVEKFVFQIINDSTGKIPESFLNKNLEVSTRTGLNSIIPQD
                     FKIKKTLSSIYNLTDMEIKNIEVVVKETDLGVGNMIDDHAISLLRLTPD"
     gene            complement(16104..>16307)
                     /locus_tag="VF222_14395"
     CDS             complement(16104..>16307)
                     /locus_tag="VF222_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143664.1"
                     /translation="IPLYTSHNNFANLFYKEGYLNIKLSCFQDEYLVSPSYTKHKITN
                     VFPFHFINSISPIYLINLLCPPN"
BASE COUNT         5876 a         2260 c         2520 g         5651 t
ORIGIN      
        1 tggaagggag gttgaagaat gtacaccttt agaaataact gaaatattaa ataacgcagt
       61 ggacgatcca aaagaagacc cattctcaac tataaaaaaa caacaatctg tattacaaaa
      121 tcataaaaaa tataacaaaa acgacagaga aaaaacttct tttcagcaaa cacaacccca
      181 gtttaatcaa ttggaaacat atccaattac taaagaagat aatgggttac aaactaataa
      241 tgaatccaag tcagttatta gaaacatata ttctcagatc aatgaaacct tagaagcaat
      301 tattctggat ggctcaatga atactgttat gaaagttcca gtttctgaga tagttaaaaa
      361 actttcagat tcatatggag ggaaaatatt ggttttggat ggaatagtaa cccaaagatt
      421 gattgatgct tctgataagg ctggtataga acagatcata ggtcatagat tggcagatat
      481 aaaacggaat tcctctatta aagtaggtac attcagcgaa atgggattac aatagataga
      541 ttaaaataat taatttttat gaatactatt acctttcaac atatagtaat tctctcagaa
      601 ttgttgttgt gcggggcaaa gaataactat gttgagttaa ccactaatga gatagcaaaa
      661 aaaataaaac gctcacaaca attagcttct aaagaattac tcgatcttga attttttggc
      721 catataagta gaaacaaaaa aagtaaaaaa tatactataa aggttacaga aaaagggtat
      781 caacaagtat atgatctctt tttatttcta aagactgcta tagagtcttt taaatatatt
      841 atcgattttg aaggccatat tataactgga atgggagaag gtgcgtatta tatgtcatta
      901 aatggatata aacaacagtt taaagaaaaa ttaggttatg aaccttatcc cggaactcta
      961 aatataaaat tgaattctat gttgtatgta gaggctaaaa aggagatgtt aaaatatcca
     1021 tcaatagata ttgaaggatt tagtgatcaa tctagaactt ttggatgggt aaaatgttat
     1081 cctgcttata ttaacgatag cgtggatata aactcctcaa ttttgatatt ggaacgaact
     1141 cattatgatg attctataat tgagataata gctccatttt ccattaaaga acaattttct
     1201 ttgaaaaatg gcgattatat caaattgaaa gttaacacta attatcctat ctaacgaatt
     1261 aaatttatat cttgtataat gaatctccta aatctcgttt taattcagaa ctcttcatat
     1321 ttggaattgg ggtatttaac cttaatattc taacgttgat atttcttttc ttgcagttct
     1381 ctgaaatgaa tttctctgta tgtgattgat cataacctaa tgctatgatg tcaggtttta
     1441 catgttcaac tgtatcaaat aatgtacctt cttttccaat taatgccaaa tctacaaaat
     1501 ttaatgacga aaccatttct cttcttaagt tttcattatg aaatattatt ctattttttt
     1561 ttattttagt tgctgtagat tctgttgcaa ttattactat cagaacatca ccaagtaatt
     1621 ttgctgcctt gagagtaaat atgtgaccag gatggagtat atcaaaaaca cctcctacaa
     1681 ggactacttt catcgtctct cttcctaatg aatttaattt atattgttca ggattaattt
     1741 ttcctattaa tcccaattct attagttctg tcattcttga ttcaaaaata gatttatcta
     1801 attgatattt ctcttgcaaa tatcgtaaaa atgattttga tggatctaaa ttactaatgt
     1861 atattgcttt caaaataagt ttatctagtg atttcaattt tatcccgaga tttttaaata
     1921 tgatatttca taaataatat atattcgttt gaggacaaaa tagatgtacc ttattcattt
     1981 atgaagcttt ttttctagtg ttttctaacg ttgaacaaat caaagctttg tctaatatct
     2041 tctcacaata cttttataag gtttatatat attttatatt ttaacttggt taaattttgt
     2101 cctgaatgtg gaggagattt aaaaatggat ccatctacaa gaaattttat ttgtaaaagt
     2161 tgtggcctgt atgcttccag agacaaaatg gaggaattaa aagacaaatc acaaattggt
     2221 tcttcagttt ctaaacaaag aactcatgac gattatttgg attggtggca atcctcaaaa
     2281 aaagaaaaac aaagaaatta gtcaattatt aaaaaaattc ttcggttttt tcactcattt
     2341 ctttttgtaa cgagatcatc tgttcttctt ttctcgttct taaaactgtc attaactctg
     2401 tttcctttac gtaattactt gataatattt tattcacaat tatatctctc aaattgtcca
     2461 aattttttga tcgaatttta attaacaaat caaatttgtc atagatttca tgtatctcta
     2521 aaacttcatc gatttccata aggttttttg tagtttcctc tgtttttccc ctttcaattt
     2581 ttaaattaat aaatgctaga tggtcgtacc caagcaaatc attatctaca aaaatagtaa
     2641 atttttttat aacacccaaa gtctgtagtt tcttgactcg agtatgaata gttgcatctg
     2701 aaataccaat ttgtttagct aattctacaa atggtaatga ggaattttta cttaaaacat
     2761 ccaagatcct acagttaata taatctaaca aatttggttt gatattcata attcttcaga
     2821 tatagtacta aaatatatta atattgattt aacccatgtt taaagatatt aataatctat
     2881 atacatcacc ggtaaataaa atacaaaaac attgataaac agtagttttt tgatagtcta
     2941 gtttaaatta aatacctatc caatacaaaa aaaactgtca tataaatgaa acattcccta
     3001 tgttcaaaat gtcaaaagat agaaggtata tattttcgac aatattcagg tgaatatctt
     3061 tgtagatcat gttttataat ttcaattgaa gaaaaaacct ctaggactat ttctaaattt
     3121 tcaatggtta actatggaga taaaattgca gttggtgttt caggcggcaa agacagtctt
     3181 tcgttactat tagttttgac ccatatcttt gaaagaaaga aaactaatga tattattgca
     3241 attacaatag atgaaggaat tgctggatat agaaatgaat ctttaagaat agttaaagac
     3301 ttttgttcta aactaaatgt aaaaaatgaa atctttagtt ataaagaact ttttgggatg
     3361 gatatggatg aatccatgtt gctacgtcca tcagaaaaaa tgacttcctg ctctatatgt
     3421 ggaaccttta ggagaagagc tatagatgta gctgctgatt atgtgggagc aagtgttata
     3481 gcaacagggc ataatcttga tgatcatctt caatctttta tgataaatat tatagcagga
     3541 gatgtaaata gaataggatg gatatatcca gaaccaattt attatggtaa aaatgaaata
     3601 aaaaaaatta aaccattcat agaaatttat gaacgagaac tagtttttta cgcacttcaa
     3661 caaaatattc catttcaatc cgaacagtgt ccataccaag atgaaagtat taggagtgag
     3721 ataagggaat tctttaattc tttagaggaa accagatcgg gtgttaagta taatacctat
     3781 aattctcttt tgaaaatatc caaaatatta aaaaatcatg atagttcagt gaaaaataaa
     3841 tgtagaatat gtggacatga ttcgactaga tccgtttgtt cagtttgtaa aaatatcaaa
     3901 attttgcaaa atgactaata aacaaatata acgtaatatg gttgaataaa gattgcggag
     3961 gcagggagga tcagggtgct agccgtaccc ttctctggaa atcggcttta tgctaggatc
     4021 agtaactatt agataaatcc ttggatagaa atctcctgcg tatcaagtta aaatgaaacc
     4081 acgccgaagt ggacggatat gagcagcaaa ccatgcgaag gtttggtatt gactgttaat
     4141 tctcgaaatg ggtgaggtcc gggagggagc aaccctaagg tgacataacc gtgctggttc
     4201 gtctacgtgg tggatcatat gagatgcgag attggggttt ttatcaaagg tggtactaat
     4261 aggctgcctt cgcattcatt taataattta gaaataatat tcctggatta tcttaaggca
     4321 atttgctata ttttcataac caatgacgta gctttaaaaa atcatccaga ggttcgtttt
     4381 ttaatacctt aattaatgct tcagcttggg ggacagaaag taatggttta ctaaaatgat
     4441 tgtctgtagc tattctggga caagcaactt gaatataagc atctattcct ttaaaattcc
     4501 ttaacctttc ttcagtaatt tctgttaatg caattaattg tatatccttt cccaggactt
     4561 ccaaagactt cttaaatttt aatgctttta tctttgagaa ttggccttct tttaatccta
     4621 ttattattcc aattttggtt gcttcttgcg ctttataaat tgataaaatc gttttctttt
     4681 ccataattct ggccttgtca tttacttgtt gaaattcatt aaagtatgga tccaacatga
     4741 aagtaggctt ttctgttgac attgcaatac ttacagagtg aaacatactt tggcctaaga
     4801 aaatataggc atctactctt ttttggatat catgtgctgg ataaaattca cagccaaaaa
     4861 cttgaccatc atttaactgt cctttaccat caccaattac tattttaaat ccatgatttt
     4921 ctaatatttt tctagaaggt tgtaattgat ctaagtgttg gctatctgta agtataccta
     4981 atattttata ttctttattt gccatatctt tagcaaattt ttctactact atatcgaatt
     5041 tcacatcatc atatgcattg atcataatta cttttttatc aatagtttcc atagaaattg
     5101 tatgtccaat gttaaacaac agatctgcac ctaacatttc tgccgcatgc gtattgacat
     5161 cgcaagatcc ccaacaagta tcacctatga tataggctgg aatctcatat cgtttcataa
     5221 tcttcattcc tgtttcttgc atcttgggga ttaatgcctc cggaccattt agcgctaccg
     5281 ataagggttt tgtttttctg attacttcaa aaattttatc ttcatcaatt gaaatcaatc
     5341 tacaatctca tctaacttaa taatctctcc aatattattt ttcatatcat tatcaataat
     5401 attagtctaa attatttttt agaaggaaat aatacagaaa attatagatc tgagtcttga
     5461 caaaaattgg agatgtttct atcaatagga attctgtaaa aatcatggga ataataaatg
     5521 ttagtcctga atctttttat aaaaattcaa taaaaacaaa tctcaatagt atatcttctt
     5581 atgtaaaaaa attagaaaat gatggagcag atataataga tttaggagca atgtcaactg
     5641 ctccatatct agataccgtg atttcttcga aaattgaaat cttgagatta aaaaaagcta
     5701 ttatgataat acgtcacaat tgtaaacttc ctatatctgt cgatactccc agagctagag
     5761 tagctgagga ggcaataaag ataggagcaa gtgcgataaa tgatgtttgt ggactaaaat
     5821 atgatcctca aatggctgaa attatatcaa aacataatat tccagtaata ctgggggcat
     5881 atgaaaaaaa acccagtaaa attctaacgg ctaaatcttt ttcaaccaag aggattcttc
     5941 ataatagtat tacaattgca caaaaagctg gtattaataa aactaatatc ataatagatc
     6001 catcaatagg tttttttaga aaagaaggca ataatccatt ttttacaaaa ataaccacac
     6061 catggtattg tcgagatata gaaattgtct ctaatctgaa agaactcaca aagctctctt
     6121 ttcccatttg tatttctctt tcaaataaat cctttttagg aaaacttatg aatctcagga
     6181 taaacgaaag acttatccca tctttaatta tggaggttat tgctgttatt aatggtgcaa
     6241 ctgttattcg cactcataac gttaaagaaa cagtattagc attaaatact ttacaattgc
     6301 taaattagca tattttaaat tgctttttca ttatgaaatt aataacttgt tcaatttaaa
     6361 aggttcttga ttattaaatt agattgatgc aaaacttata attcttcctt aacttaataa
     6421 gaaaatgaat attgaatatt agagaaggtc taacttttga cgatgtatta ttaatcccaa
     6481 aacgttctcc aattatttct cggtctcaga ctaatttaaa aaccagatta tcaagaaaca
     6541 ttagtttgaa tattcctatt atcagtgcca atatggatac agtcacagaa tcacctatgg
     6601 ccatagcatt agcaagagaa ggaggtattg gaattataca tagatttatg acaatacaag
     6661 accaagtaga tgaagtatta aaggttaaaa ggtctgagtc agttatgata gaacaaccat
     6721 atactattag tatagattcc actgtaggct atgcaaaaaa gattatgcat gattttggga
     6781 tatctggtct ccttatagaa aaagataaga aactagcagg aatcatcact aaaagagatc
     6841 ttttatttga aactaatttt gaaaataatg tttcttctgt aatgagtaag gatgtggttt
     6901 tcgctgaatt gggaactaca attgaaaaag ctaaagatat tttacacaaa aaccgaattg
     6961 aaaaattacc tataattgat aaagataagc agatcatagg gttaataact agcaaagata
     7021 ttttaaaaat ggaagagttt cctaatgctt ctaaagataa gaaagggcgt ttgcttgttg
     7081 gagctgctgt gggggttaaa ggagattatc tcgagcgaac agaagcatta ttagatgcag
     7141 gtgcagatgt tatggttgtt gatatagctc atggtcatag tgataatgcc atcaattgtg
     7201 ttcatttaat aaaaaaagca ttcaaagatt gtgagttgat agctggtaac attgctactg
     7261 gccatgggac ggaggattta attagagctg gtgtcgatgc tgtaaaggtt ggtgtagggt
     7321 caggttcaat ttgtattact cgggttatta ctggatccgg ggttccacaa ttaaccgcaa
     7381 ttttggatag tgtaaaaatt gctaaagaat atgaagttcc ggtaatttct gatggaggta
     7441 ttagaaattc aggagactta accaaagcac tagcagcggg ttcttcatct gtaatggtcg
     7501 gaagtctatt aggaggaacc gatgaaagcc ctggaaaaac actggttaaa aatggtaaaa
     7561 agtataagat ctatagaggg atggcctctt tttatgcttc cttgggaaga aaatatcgtg
     7621 aagagggaga acaattaata gagtcagagg atcttaacga ttatgtacct gaaggcgtag
     7681 aagcaatggt atcttataaa gggagtgtag ttgagattat ccgtcaatta gttggaggtc
     7741 tgcgttcagg gttgagttat tgtggtgcaa aaaatatcaa tgaaatgcaa cagaatgctg
     7801 aatttgtaca aataacaaca gcaggttata ttgaaagtca accacatgat gttaacgtta
     7861 tgtaaataat ttattttttt ataataataa taggaaattt ttgattctga cttaattatc
     7921 ttctgatatc tttataatta tgcctcttgt aaatggaact tttaatattt atattatagt
     7981 tgataaagac tagatctatt aaattatata atgttattta ttaggttata tattgtctat
     8041 tgatgtaacg gtccaggtat taaaggaaaa tgtgggaaag accataatga ttaaactaaa
     8101 aggagataaa gtggttaaag gtatcctcaa taaatttgat caacatatga atcttgtatt
     8161 aagcgatgct acagaatata aagctggaga tcagactaaa ttggggaata ttctccttaa
     8221 aggagataat ataataataa tatctccaca agaataatac aaaagttatt ttgtttttct
     8281 ttatgatcat tataacctga ttaattttta ctgtaggtta ttctttcggt gcgtggactt
     8341 tggtcttcat ctcatgttat acataatcag ttcgcccatc gattgccacg caccgatagg
     8401 aaaactgaaa gatgacccga aaattaaact gtatggatta taaccatttg tttatcagca
     8461 tcaactataa ctttggtatt attatttaat gatttaaaac atgaacctgt tccatctaca
     8521 agtggaatgt ctgatattgc gcatcctgat gcagttataa tgtcacattt attggtacaa
     8581 ataattgcta ttgggctgac accattcatt tttaatgaat aaaaaatata agctcctaca
     8641 ctacttccaa tacagttagg aaaaattaga attttatttt taagtgtttg gttaaataga
     8701 tcatggtctt tgtcatttat ttgtccggat tttgtatcta acattgagag catatttaag
     8761 ggcttattcg ataaaagcgc taaaccttct gccttcccac caacaatttt tttgcaattt
     8821 atctttatca taaataaatc attcacaata ttcttttgtt atcgatttca tatcttttaa
     8881 agaaacatct attttgtttg aatgtttcat ataatatgca cattttatac tattagttac
     8941 aattgaatca tactcatttt ttgttattaa tggagtcaaa catgtacagg aatcacaagt
     9001 aatatcggct cctgcctttt ttaatttttt aatcatctta ttattacatt ctgttactga
     9061 acgagggcaa aatattatac atggtttttt gaattttttt ccctttgtta agtgaccaag
     9121 taggtttact tcatctaaac ctaattgagg actcccaaat actattaaat cccctttttc
     9181 ggcagtattt aaatcatcct ttacttgtct tagctctgtt ttcccaaaag agattttctc
     9241 acagtcatta tttttattag taaacatccc acacatacct gatgttccaa ttcctgctga
     9301 aatagctttt gacttgataa tatctagatt ttcagattta atttccaaac ctacacaaat
     9361 atcctttgat atttttccag caaagtaacc taacagtcca aagtcagact cggtttttat
     9421 gtctatgtct ggttttatac taatagtcgg ttttctaaga tcatcaatta ataaatcaga
     9481 gtagggagtc ttgccagtta ttgcacttgc taatgagctc aaggaacttt ccttgttagt
     9541 cttgattcct aatatagaat ttacgaatat cgctgcatta ctttctgcaa aactaacgat
     9601 ctctcctttt tttggcagat cataaatttc ataaggtata caagtgaaag aaggtttcac
     9661 acctaaagat aaataagaat ttataattga cagttgattc gctttaaaat ctcctgaaat
     9721 gttattgtct ttctttgagt caaatcccat aggatttaac gttgttgtga ctgtaacttt
     9781 agtttcttta tctaactttg aaagaaatct tactccagcc tctcctattg tgttaaaatt
     9841 aactcccgaa atatgggccc acttaatagg aattaatttt gttgcattgt tagcatttcc
     9901 tattgcaaca agtattttat aggctaactt caatccatca ccatattctc cgtgtaatgc
     9961 cttctcctct tcttttgtaa gctccattct gtaaattcag aaaataaaca tatcttgtca
    10021 tttaaaagtt cttatgatct tttatatagt aaatataata ctttaaactg atctaacaaa
    10081 tagtctatta ataaggtgtt aattatatta gatgtgcact ttgtaacctg gttcccctat
    10141 tatcaaggca ttagagaaaa aatgggttat agtactgaag aagatcaaaa ggctgcatat
    10201 ttattatcaa atttaataaa acgaaagttt ctagacccta gagttttgaa taaaaaggta
    10261 aaaggtaaac gaatattggt tatcggtgca ggaccgaatt tggatcttta tacttctttt
    10321 atcaagaaaa atagatcatt agttaagata gtcgcagatg gtgcagtaaa atttttaata
    10381 gaaaataata tcaagcctga tatagtggtg acagatcttg atggtgatcc tcgttttttg
    10441 ataaaagcag aaaagcttgg ggcaattata gtaattcatg ctcatagtga taatatagct
    10501 ttaatggaaa aatatgtacc taaatttaga agaattattg gaacaaccca agtaatgcct
    10561 gttccacacg tctttaattt tggaggattt actgatggag atagatgtgt attccttgca
    10621 gaggaattta gagcaaaaga tattgtatta gttggaatgc tatttgatga cagtataggt
    10681 ccctattcaa aagaaataat aaataacgtt aaagttaaaa gagaaaaact acaaattgca
    10741 aaaaaacttt tacaggttct tgctaagaat tcaaaatcaa atctatttga tacttctaaa
    10801 aagcctataa ttggttttaa accttttaaa atcatgcctt aatttttcta aaatggtaaa
    10861 tgatttttta ttgtaattaa gatttaagta aaaaaataat tcgttatttg ttcaatcatc
    10921 aatatgattt tataataatt aaatttattc caaatctcat tgtcatatta tcttattaac
    10981 ttttagagat ttaattagtc aaaaactact catcatacta actagcataa ttaatgataa
    11041 agtagccttg gtttaacacc aaataattat tatttatata tatttatata ttttttttca
    11101 ccaatatctg gtattagctt ttagttatgg tagtatttct tctattaatt attctatata
    11161 tcatctataa tgggttttgg ggaaaagtta attcaattga tagaaggggt tattattaaa
    11221 atgctttaat tacatctatg aaaaattaag taaataaact atgacaaaca tgtagattat
    11281 aattaaaaat accatgaaaa tagtgttagc taatgcaaat agtattgtat cttgtttttc
    11341 ttgacactaa ttctatatat cttaaatttg caaacaatta tcaaatagaa tattaattta
    11401 tttatcaaca atttaattaa tcaatatata aatatgttag aattttaata ttctgttttt
    11461 attataaaac aatatataat attaatcgga agggaaaaac aactttatat tctttaatta
    11521 tatgttacat tgaatgttaa attattcaga agaggatatt aaaaatgctg cagaattgag
    11581 agactgggta ttaaagcagc tctcagataa gcaagaagaa atagatagac ttaagcgtct
    11641 tcttctactt atcgataact tattgaaaca ggaaagtttt aaacctgctt ctaaacttga
    11701 acgttcacta aataacacgg aaaattctac tatcagcgaa agacaagaac cggttattcg
    11761 taacaaatct attgtaaaaa ctgattacaa aaaacataat ctcaataatt taaatgaaaa
    11821 tgtatccact tttactgaaa caagggagct taagagactt aaagataatt tattattagc
    11881 aaaagtaaat atttcttcac aaacaattga aataattcca gagaaggatc tattattcag
    11941 tattgatact cctccattca aatcattttt tataaaaagg attctagaag gaatgatcac
    12001 tcaagatcga gataaaaatc gccaaaaaca gtctttgaat catgaacttt tgaactatat
    12061 tattgatgaa catgaaggaa gaattaaaaa gataataata aacaattata gagaaaaaga
    12121 aagactaact gatattatta atacttctgc atgggtcttt acacgaatgt tagagaaaga
    12181 taatactttc gtaaatagaa cgtaaattgg ataaaataat tattctagat tttggatctc
    12241 aatacagtca tcttatatgt agaagaataa gagatgcaaa tgttttttgt gaaatttacc
    12301 cttataatct caagtctgat caaattaaag agctatctcc aaatggtata atcttttctg
    12361 ggggcccagc tagtgtatac tctgaaaatt cccccaaacc tgataaaaag atctttgaac
    12421 ttggaatacc aattcttgga atatgttatg ggcatcaaat aattgttgac aatttcaaag
    12481 ggaaagttaa aagatcttat gcccgagaat atggaaaggc aattttgaca atagatgata
    12541 aatcagacct ttttattaat ttagacggaa gtataaattg ttggatgagt catggtgatg
    12601 tagcagaaaa gactccaaac ggatttaaaa tattagctca cacagcaagt tctttcgcag
    12661 cagcgattgg gaatcaagaa aagaaattct acggaattca gtttcatcct gaagttgttc
    12721 atactgagcg tggttttgaa attttgaaga atttttctat caatattagt aaagcaaaac
    12781 ctgaatggga tatgacaaat tttgtcgaat ctactattga gaatataaag aaaaaagtgg
    12841 gaaatgataa aatattatgt gcagttagtg gaggtataga ttctactact gtagcagttc
    12901 tattaaatca tgccgttcat gataatttgc aatgtatctt tgttaatcat ggtttgctta
    12961 gatatggtga agaatctatg gtaatgaatc ttttaacaaa aataggtcta aaaataacct
    13021 atattgatgc ttcagagaaa tttttaagca aattaaaagg cattacagat ccggaagaaa
    13081 agaggaagat aataggtcac gagtttatta acgtttttca ggaggaagtg aaagaaaaag
    13141 aagaatttcg atggttagca caaggaactt tgtatcctga tgtcatagaa agtgggattt
    13201 ctctaggacc tgcatcaata ataaaaactc atcataatgt tgggggcctt cctactctac
    13261 ttaatctcaa ggtattagaa cccttgagac atctttataa agatgaagtt aaaaaagtcg
    13321 ctaagatcct agagattcca gatgagcttt taaatagaca tccatttcca ggaccaggac
    13381 ttgctgttag aataatagga gaaattacat cggaaaaaat aagaatttca aaacatgcaa
    13441 gtaaaattgt tgaggatgag cttactactg aaaatttata taattttgtt tggcaagcct
    13501 atgccgcggt aggagatgac aaagccgttg gggtaatagg agatgaacgt ctctatggtc
    13561 atattgtaac tataagaata gttaactccg tagatgcaat gacagcagac tggacaaaac
    13621 ttccatatcc actccttgaa agaatcagta atagaattac aaatgaagtg gatggagtat
    13681 catgggtaac atatgcaata tcaagtaaac caccagcaac tatagaacca cagtaattta
    13741 catattatga tttagtaaat ctatgagtta ttttttatca agtaatttat agatttagtg
    13801 aatattccaa aaattgctat gggtgttata aattggaatt ctaaatgaag aaatagttaa
    13861 cgttaacaag taacgaaaac cttcaacgat atagttatat agtttcaata tgtgtaatgt
    13921 gaaggaacta attttattgc ctatatcttt ccttgaggtt aatcaataga aatttatttt
    13981 aataattaag cattgtatta tcttaagtct atcttaataa ttagtgccaa ccattagaat
    14041 tgttatgata ttactttggg ggagatttat gtaataatta tgatttaaaa aacgctatat
    14101 ttaatcccat ttggccatta aataaattac tattgatata ataaattgtc aaaagaaaaa
    14161 ttaaacgagg atcaaaaagg tctatattct atataatttg ctatttattg tatattaatc
    14221 gtaatagtat attagaatta ccaaaatatc aactaaatta ataaagaggt tatatactat
    14281 atatcttatg tctgaagaat ctattcggtg tccatattgt gaatcattat ttaataataa
    14341 tgaggaactc tctaaacata ttgatagaat ccatcttggt tctggccttt tagaaggtaa
    14401 ttcaaggaaa ttctaacata tacttattat ccttatctaa aattcaaacc ttctatctct
    14461 cacttcaata tttaatactc ttgtatttta tttaattttt tgaccttgga aagtataaat
    14521 tatattatat tttctaatta tagtattaat gaaaatttca attttatttt ttttatttat
    14581 tacattctta atttttatat atgtagaaca tataaaattt gaaatagttt atacatcaac
    14641 gaatagttca aagtccttaa atatcgaatt gatttctatt aacggttctc ttatttcggc
    14701 tggcaaattt ctcatcattc ctgatcccta tcagaaattt gcaagcctag aaatacatga
    14761 taatgaattg gtggattcaa ataaaaccat aggagttatt tctcttagag gtatttccaa
    14821 taataattat acaattacac aaaaagccgt taataataat tatttaccca atacaatttc
    14881 aaaggtagtt gaaattaaag atgatagtcc aatttcttcg gttttatttg taaataattt
    14941 taattttaaa tctgaaaaac ctttgagttc tacgtctaat cttttcacat ataatgctaa
    15001 attcgtatgt ggttctatca gaggtaatga aggaccatta cgaccaggac actatgatac
    15061 cgatatcagt atttataata aacagaatta tccaattacg ttattttgga atgtagtatt
    15121 caataattac aattcgagca attccataat aaagaccatt gatccaaaac aatcgactgg
    15181 attggtgtgc aaagacataa gaaagttagc acatttaaat gctcaagata ctgatttaat
    15241 tgaaggattt gtaataatcc aaattgaaga aatgaatata gcttctggaa ataaggcaag
    15301 ttttgcaaat gaaccagtaa gtgtacaggt gttttatacc gccaatgcac ttgattccct
    15361 tccacaagaa actttagtag aaaaatttgt attccaaata ataaatgatt caacaggaaa
    15421 aattcctgaa tcattcctaa ataagaattt agaagtatcg accaggacgg gcctgaactc
    15481 gattattcca caggatttta agatcaaaaa aaccttatct tcgatttata atttgactga
    15541 tatggaaata aaaaatattg aagtagtcgt taaagaaaca gaccttggcg taggaaacat
    15601 gatcgatgat catgctattt cattattgag actgacacca gattaaaaaa taatattcta
    15661 ataaaaacaa cgttaattta ttaattgttt tttttattga aggtttagaa aatggaaatt
    15721 aaataaattg tattatcaaa taaattacta aaatctttat taacgatatt aaggtgttct
    15781 aatttgattc ttttttaata aaatggataa atattcattt actaatctaa ttttatgtat
    15841 ttcataatat attgtaaaag aagaatcctt aaaagtattt aatttgatta ctagaagggg
    15901 gttagaaact aaagataaaa ttgattaaaa tttactatat tctaacttta gacataataa
    15961 tcacaatgat attacttcta caatatgcaa atactcaaat gaaaatagat tctactttaa
    16021 ttatatatat aaaacaattc aaattcaaaa aattatgtat ttttagatcc tacaaataca
    16081 atactcgatc aaaagtaggt gtgtcaattg gggggacaca aaagattaat aagataaatt
    16141 ggtgagatgg agttgataaa atgaaaggga aaaacatttg ttatcttatg ttttgtatat
    16201 gacggactta caagatattc gtcttgaaaa caggaaagtt ttatatttaa ataaccttct
    16261 ttatagaata aatttgcgaa attgttgtga gatgtataca atgggat
//
